LOCUS       DAOOWY010000001        54687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_640_length_54687_cov_11.094066, whole genome shotgun sequence.
ACCESSION   DAOOWY010000001 DAOOWY010000000
VERSION     DAOOWY010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 54687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54687
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_640_length_54687_cov_11.094066"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PK105_00005"
     CDS             complement(<1..171)
                     /locus_tag="PK105_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60114.1"
                     /translation="MNKNQSQPQFSPSRKIPVTILGATGVVGQRFLRRIANHPWFYPQ
                     FLAASDRSAGKKY"
     regulatory      complement(296..479)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            615..2144
                     /locus_tag="PK105_00010"
     CDS             615..2144
                     /locus_tag="PK105_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOU60115.1"
                     /translation="MIQSCYSSFSELEVAASDALLELSRMLLRIGEPEEVLGYFLNSI
                     LPLFHGRAYAACYRADSGDVLRYFQVGPNEDSWFDENVRIGEEVCLGSVPCGLALESD
                     KFVQKKLNRSLILEGPIKQRDNTIFAISILLSGDCASEIVSLASETIKRAAGIIEEFL
                     GMRSRLAEKIDRDNGRLIGVIDSDLSESPVSWYGIVGNSDVMREMFSMIDQVADTEAT
                     VLLLGESGTGKELIAQAIHQRSSRASKPFVAVNCAALPESVIESELFGHEKGAYTGAF
                     EQRKGRFEQANKGTLFLDEIGELSTALQIKLLRFLQDHRFERVGGNENIEADVRVIAA
                     TNRNLQQAVDEGSFRADLYYRLNVFPINVPPLRERGADILLLADHFVSQFNQENSKKI
                     QRISTPALDLLMIYHWPGNVRELENCILRAAILSTDGVIHAYHLPPSLQSAASTGTEP
                     KTGLDAEIFRVEKELIIEALKLENGNVSAAARRLVITERRLRYAIQRHRIDPRRFKTK
                     L"
     gene            2495..4648
                     /locus_tag="PK105_00015"
     CDS             2495..4648
                     /locus_tag="PK105_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOU60116.1"
                     /translation="MDEHSSNFSLIDHFGSRCFSNDVMRERLPRSAFEAILEIQAGKR
                     ELTLDIAEIVAAAMKEWASDLGATHYTHWFHPLSGLTAEKHESFVSPQPNGSILMEFS
                     GKDLVKGEPDASSFASGGLRATFEARGYTAWDVTSPAFLKRNAERYILCIPTAFVSYT
                     GLALDKKVPLLRSMEALNRAGLRLLRLIGDSKTERIIAYAGPEQEYFLVNAELYAKRP
                     DLKLCGRTVLGTMAAKGQELEDHYYGAIDEKAAAFMNELNSELWAMGISAKTQHNEVA
                     PNQFEIACIYSQENIAADANQLVMETIRKTAEHHGMAALLHEKPFAGINGSGKHINWS
                     IGTDEGLNLLDPAIVAGISSEEEAGFKFIRFALFSLAVIEAVDRRAGLLRSSTATAGN
                     DRRLGGHEAPPAILSIYLGKPLYDLFNSLQNQNNAQRITNAFLELGIRSLPKLPRDFS
                     DRNRTSPFAFTGNKFEFRTVGSSQSAATSLTMINAAVAEALNRISEEIEQELTKGAHL
                     LDAARLVAQRSWQEHRRVVFNGNGYSIDWMHEAEKRGLPVFRSAVDAIQELSTPDNVK
                     MLEDLCILLPEEIEARQHVYLERYSKQIKIEAAMMIDLVRRYIIPAASEAASNYGSIC
                     SSIAAIGAPAIVQEALAKRIAQLVGKSAEGLDRLERALSDASCTEDELERAKSYRDMV
                     VPSMDELREFCDELERLVPKYLWPLPTYSDLLFTL"
     gene            4778..6268
                     /locus_tag="PK105_00020"
     CDS             4778..6268
                     /locus_tag="PK105_00020"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU60117.1"
                     /translation="MKFLRRIIPWILVATVILAALYIPDLGPENYRDFDSLFRNERVR
                     QGIAGYEVVIIKGGEIVFNKAYGFDGQRNPLDISTPLYMGPASEIFTGTLLAQLVNEK
                     KITLDVPITHFIPELGSLKARTASDDSLDLPLTLRLLAAHRVAFPEKDLPEFDPETMG
                     LEAGLPDADLFLSSHFPTEKYTRSRLSYRIVGTILEKASGMKYSELLESKIAVPLDMR
                     STTAEPSSMLSIAVGAGSFFGLTFPYVEKVPYDAAPSDGIVTTSEDLAKFLRFVVSPK
                     RGQRIPGLSSAQTPMLYQPLYKDGDTGFGWRIIENKEGRSIFQGGSIRGYASRIVIYP
                     ERNAAIAILCPQDGVLISNFLLPKLVSCAEQILFEGESGRPFPTQRAQLVLGFIFIIY
                     LLNIILQTLVSYSWARNLLKYRETGISQLYARFVILRTIIGLVLRILIVLLAPLAIGA
                     LVGWQVTYQEIIAFEPGVSTMLMTALLFGALRNIARLVMVLRLRHF"
     gene            complement(6306..7016)
                     /locus_tag="PK105_00025"
     CDS             complement(6306..7016)
                     /locus_tag="PK105_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60118.1"
                     /translation="MLEIKNLSVFYGGIHALQGIDLDVPDRKIVTLIGANGAGKSTTL
                     RAIAGLIKPSSGEVILQGSVISGRPTLEIVQKGLALVPEGRRIFSNLSVEENLLLGAY
                     SRTDKKAVLADLEHVYELFPRLKERRGQKGGTLSGGEQQMLAVGRGLMSRPKLLMMDE
                     PSLGLAPLISKMIFEIIKQINSEGTTVLLVEQNAKAALEVADYGYVLETGRISLFGTG
                     HELLQDDRVRKAYLGEAQ"
     gene            complement(7016..7792)
                     /locus_tag="PK105_00030"
     CDS             complement(7016..7792)
                     /locus_tag="PK105_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60119.1"
                     /translation="MPELPELMLSTSHLTMRFGGLVAVSDFNLEIRKNEIVGLIGPNG
                     AGKTTAFNVITGMLVPSEGKVLFKGKDITGLKPHSITALGIARTFQNIRLFKEMSVLE
                     NVLVGCDLSINTGILAATLHLPGYRTRESEARRFSVELLEQVGLAHLANEKGTSLPYG
                     QQRRLEIARALATKPKILLLDEPAAGMNPQESFELMDFIRKIRDEFDLTIFLIEHHMQ
                     VVMGVCERMYVLDYGITIAQGNPSSIQKNPKVIEAYLGVD"
     gene            complement(7776..8894)
                     /locus_tag="PK105_00035"
     CDS             complement(7776..8894)
                     /locus_tag="PK105_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU60120.1"
                     /translation="MSKKSSTRHILKDRHIENGAISKNMLYTIIFLGACALFIFLADR
                     LFDAFSLRVFNLCAIYIVLALSLNLLNGFTGLFSLGHAGFMAVGAYVSALLTMPAPLK
                     EMNYFLLPIVPWLQHVTIPFLPALIIAGLVSACVGFLIGAPVLRLRDDYLAIATLGFG
                     EIIRVIITNAQSITNGAQGLKGLPRFSTTFAVWAAAGLVVLFMVLLMRSSYGRAMKAV
                     RDDELAAEAMGINVFRVKVTSFTVSSFLAGIGGALLGHMITTIDPKMFTFTLTYNILL
                     IVVLGGVGSISGSVISAIIVTVLMEALRFLDEPINLIFFEIPAMPGLRMVVFSILLMV
                     MIIYRQRGLMGNKEFSWDMFQSAFVKKSKKGGSHARTP"
     gene            complement(8897..9790)
                     /locus_tag="PK105_00040"
     CDS             complement(8897..9790)
                     /locus_tag="PK105_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU60121.1"
                     /translation="MTLSLLFQHIVNALSLGSLYALLAIGYTMVYGILRLINFAHGDI
                     FMVGAYVAFYGITLLSLPWWAALLLSIIFTAFFGIGLERVAYKPLRDSPRISIMISAI
                     GASFLLESLAVLVFGGRPKGMPVPDVLSKVIYFGSVSMITISLIIPLFTFALLILLLW
                     IVNKTKIGMAMRAVSTDIEAARLMAVDVNKVVSVTFGIGSLLAAFGGIMWSFKYPQLN
                     PLMGVMPGLKCFIAAVIGGIGNITGAVLGGFLLGIIEIMMVALLPTLSGYRDAFAFIL
                     LIIVLLLKPTGLLGKRQAEKV"
     gene            complement(9939..11090)
                     /locus_tag="PK105_00045"
     CDS             complement(9939..11090)
                     /locus_tag="PK105_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU60122.1"
                     /translation="MKRIVRIGLLSLGIAALIVACGPGAPKTVKFGVFEPLTGANAGG
                     GALEVEGIKLANELYPTVMVGDKEYKIELVIADNKSDKVEAANAAQRLVDKDKVNVVL
                     GSWGSSLSMAAGPIVKNAKVSAIGLSCTNPLVTKGNEYYFRVCFIDPFQGTVMANYAY
                     KTLGAKKAVIIREVSNDYSVGLAKFFADAFIQLTGDEDAILAELNYNTGDTDFSAQLT
                     EIKKHKPDVIFAPGNYTESALIIKQGKELGIKTPFLGGDTWETPEFIDVGKEAVEDVV
                     FSTFFATETPITETSKIFLDEYRKKYNKEPAAVTALGFDGYLVARDALQRAGSLDKEK
                     VRAAIAATKDFPGATGMITLNEDGDAVKSAVIKTIKDGKFTYMATVEPY"
     gene            11336..12622
                     /locus_tag="PK105_00050"
     CDS             11336..12622
                     /locus_tag="PK105_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU60123.1"
                     /translation="MDSYDVVIVGTGPAGLGAAFKLLESRPSFNILLIDKQQVSSGGL
                     RNDCKMNFTWPIGFPLECWDEATGKHYLELTEGFLKPHIMEKRNIEIYRKRAEKIGVQ
                     LLDVRQSHLGTDGGFKLIKDLTARLRGLGATFSFKEEMLGVEPGSRVIITDKRELKYG
                     SLVVAPGRGGFAFLQRLMEELGIKFRDNVVDIGVRIETTEEHYPIVRDYYDPKFFFPK
                     KTRTFCTNSRAAYVVQERYGDEKQGYWYSINGHAWSSERPANGLVNFAILKTITLTQP
                     LASGQDYAQMLGHLAVLLGGGRPIMQRIGDFRLGKRSFANSFTGDLYNFNPSLPSCTP
                     GDISLCIPAKTMRAIWNAMKLLDTIVPGVLHPSTIMYYPEIKLYANRPAFIDENFQVL
                     PGIYFIGDGAGTSRGITSAWASGMRAANGIISTSNL"
     gene            complement(12710..13966)
                     /locus_tag="PK105_00055"
     CDS             complement(12710..13966)
                     /locus_tag="PK105_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60124.1"
                     /translation="MKSFASLDAAGTSIGSILERFQASLADKGLSFNEFLSDAYVQKW
                     KGEQLAHALKVRTALVLGLQEFLVSRGILNIDRVSLSPVTDPLCHDVEHVPVILYQDT
                     PYRTTHSMIYSKMLACLNPYIEGIYIDSSNIRLELPSSTSRHKYLIDFSQMDIEFKRA
                     KRLSEDDYFNRPKETTQVLTKELDRALDFFEDLIIYGVKKILVLAAESLGALGVKLTV
                     PSKPFPRFYSDEIDMQGDCSLEGSLEERLGHKAKTQFFWVLGLLRENYDLVYPYLSRD
                     GRRPEKKDISPRQVFNYDLCAAPLYRDESLGAAYEILSGGLREWIYESIVSRLLDNAV
                     LRESPRFDRNGNIENMAALDGYGPFLSIAHMRDAQGQGIFPQVCGGGLGIERTLFALL
                     RGPFIKDIDEVTCFGKNPDKTLPFLF"
     gene            14377..14937
                     /locus_tag="PK105_00060"
     CDS             14377..14937
                     /locus_tag="PK105_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOU60125.1"
                     /translation="MEGNNAKLSQAIMACLFAALISVGAYVAFPIPGTPVPIVLQNMF
                     IMLAGFISGPWWGLVSVVFYLILGSVGLPVFSGGTGGFAKLAGPTGGYLVGYIPAVIV
                     FGLISKLGKGKWIFNILAGISGMIIVYAAGIVWLKSVLDIGWERAIATGVIPFLPGDI
                     IKIILASVLTPPILKAMNHIDQQTNG"
     gene            14930..15673
                     /locus_tag="PK105_00065"
     CDS             14930..15673
                     /locus_tag="PK105_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60126.1"
                     /translation="MAELFRAVSLSHIFPDKGIGLDSISLSIEEGEFLLVAGRNGAGK
                     SLLMRHFVGLSKPSAGEILYRGIPIALQIPLIRREVGYVFQDTEAQVFGLTVGEDLAF
                     GPANLGIKGSKLDAVIKKALAQAHLEGMENRSPGSLSGGEKRRLAIAGVLAMQPRCII
                     LDEPFANLDYPSVQEILEVFGTLHSDGKTLIVLTHEIEKVFSLASRLLILDRGKIVFD
                     GLPGEATIGLFLRSGLVCPYQEGYPWLIE"
     gene            15658..16473
                     /locus_tag="PK105_00070"
     CDS             15658..16473
                     /locus_tag="PK105_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOU60127.1"
                     /translation="MADRIAAGSTANPFAFEPGESKLVMFHPLAKLVFLIAATSFAMH
                     APVGFLLALFVIGILPLLKLPRAAAGPFYSIVVLMLFATIVRGIFPGDGRIFDLATLP
                     GSAVYGLRLFSIYIYSRLFYATTRVSEIGDWMTAGLRSLRKIACRREAKDNPTSRLNQ
                     ESTYYRPVSMDISLDPGMFVSLILLFLPRIFNMYRRVAEAGEMRGISISKKNFRRAFL
                     MLGQLISMGLLQSWRISMAMELRGYSPYRSIRLSRFKKQDGILLGLSAIMFLL"
     gene            complement(16478..18586)
                     /locus_tag="PK105_00075"
     CDS             complement(16478..18586)
                     /locus_tag="PK105_00075"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOU60128.1"
                     /translation="MKYMTEAGKPDHSTLRSNHALNKVKRFLYALVAGVFGILLFQWT
                     GISNKAGMALYDREMRLLVAAEGKKEDNTSSQTLRKNSGSSDSASLSPSRKKAPIALV
                     MIDQSSLDWVQNELGLGWPWPRELYGLIAYSLRNADVQAYDILFTEPSTYGPEDDSRC
                     AEAMRSAGNVVLATLTDRKPVLAVSNALYGHVVARVDSDGICRQYQAALDTDAGKIPS
                     LGLAAISAFNRKNSIADEKSISLSEAFEPLEPLAPFEAFEQTVFLKFEDSSRFERYSA
                     AQVLAGSPGTGGSDFAGLNQELNFEGKIVLIGITAPGLMDRQATPINPAQPGVEIHAT
                     FISNALSSSFIELSPIGVEVCITIIALVLAALLPTMRTRIWTIVGFVAAIIIPIGSSF
                     AFFRNLVFYNAAPAIAAGLFAFIAAIVLGYQAEGRQRAYLRRAFAQYLSPEVISELIE
                     QPKVLRLGGESKVITTLFSDLAGFTAISEKLDPERLTQFMNEYLGIISEEILAQGGTL
                     DKYVGDAVVAFWNAPLDIEDHALRACMAACHIQEKLREIGPELEKRFGISPRTRIGIA
                     TGSAVVGNLGSTHRFAYTAVGDSVNIASRLENLNKVLGTAILTIRETVAAACAGSSRA
                     SSPSSLVPAAQDLDSELAPEIRLPQLEGKTLLFRRLGPAIVEGKLQAIELWELRATEK
                     DNCAQGSLIEPWQEARHFSK"
     gene            complement(18608..19693)
                     /locus_tag="PK105_00080"
     CDS             complement(18608..19693)
                     /locus_tag="PK105_00080"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOU60129.1"
                     /translation="MRKIVLQSRVLSVILALILILPFFASCTITPESLQFFLDQALLQ
                     GMITPEQSRLILEAVRSFQIESEPFTYEEEYVIGRVVAAAVLSQYQVYDQPELTAYIN
                     KIGQGLAFYSARPCLPEGYHILVLDSEEAHAFAAPGGFVLITRGLLKLTGNEDMLAAL
                     LAHEISHTALGHGLLALTSYRMGDLAKYYVMLSTESAQSYNKEAMELFLAAVHDFVSL
                     ISVQGYSQVMEFEADAEAMRMLYEAEYRPSALGDFVVTLENSKDERFQQYSMQHPLAE
                     DRLSRIGSLAEEYSQRAELEHRKECVQRLDSLLQNEDESWHNSNLACEQGFLDSGYEL
                     AKENLIISPVSLELLRQERFEAIRPLF"
     gene            complement(19695..20186)
                     /locus_tag="PK105_00085"
     CDS             complement(19695..20186)
                     /locus_tag="PK105_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60130.1"
                     /translation="MKYRWFFAIFLALAGLTILSAQEKSLESTMLIRVEKAAVRAEPS
                     FVSPALSFAAYKTPVAIIRIENDWIFGFVRGLNRPGYIHMSALSPVKVSLNPDDVSDP
                     PPLQETEIVLAGKGFSSSLELSLRESSAFDFEAVDAMEKNSYSYAECLAFIHGIDILE
                     GEP"
     gene            complement(20381..21838)
                     /gene="sbcB"
                     /locus_tag="PK105_00090"
     CDS             complement(20381..21838)
                     /gene="sbcB"
                     /locus_tag="PK105_00090"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HOU60131.1"
                     /translation="MAVSILWYDLETFGRNPRHDRIAQCAFIRTNIALEEVAEPVVMY
                     CRLPLDYLPDPEACYIHGITPQEAEEKGLSEYEFSQRIQREMSVPGSTVTGYNSIQFD
                     DEFIRNLFYRNLMDPYSREYKNNNSRWDVINLMRAVHDLKYEGFEWPINGEGNPLFKL
                     VDLAKAQNVAHENAHDALYDVRATIGLARKVLETYPKLFEWYYRHRRRENLARLVDLS
                     RRENMLVHTSQRYTRQGGCSTVITPLGLLEDDRHALVTYDLRFDPKPFLYMSEDELRD
                     LLFSERQEDEKIRPPLNMVRLNSCPYLAPLSVLNRQARARLGIDLVECRENLRILEAN
                     PDLKARFIAAYRFERKSVDELDPEHALYAGGFYSDEDRRELERFHRVLMNSGPQAAKN
                     EFLRIRFQDPRIPTLMGRLLARNFPETLTADQHLSWKKHVYGWLQLPQEKGATALSDY
                     VRLERELQEMSPDDPRRPIAMALIKWKDRLCSQAI"
     gene            complement(21871..22254)
                     /locus_tag="PK105_00095"
     CDS             complement(21871..22254)
                     /locus_tag="PK105_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60132.1"
                     /translation="MKRHDLYVQLISELANYLLRNNPKKMVISLHLENDGFHIVAIDD
                     RYHSDEEIAEIEQGLKMHQRPELASYYGTMLGRDLLGDARLELIGYHVKGAKVEKIPE
                     GGIMINLWVGSDDFNPAMFTIDEKA"
     gene            complement(22307..23584)
                     /locus_tag="PK105_00100"
     CDS             complement(22307..23584)
                     /locus_tag="PK105_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HOU60133.1"
                     /translation="MMEQPKTLNAFMLIVLVFIFLAGIIEGGTQAILGFLQLQITPAR
                     LVSDFIAIAGVGSTLLNVSTVGFLGYGFLAINKLRLTGASLAALFTMMGFAFFGKTPF
                     NCLPIMLGVSFSALLVRKKPRDYALIAIFGTAMGPLITFIAFELGVKSFLALPASFAI
                     GLGVGLILPPIAIAMLRLHQGYNLYNMGLTAGFLGLFAASLSHATGADILPIEIWGTA
                     HSPILVALLPIVLLIALFCIVKEDPKNIVALFRHAYLDFRKILGMSGRLPSDFSDFVS
                     TKGAFLNMIVLGLSFWLFMLIIEAPFNGPVLGGLFTILGFSFFGKHIKNVFPIVLGIV
                     VAIFVFDKDLYEPGPLLAILFGTALAPLSGEFGPLLGFVAGFLHLVIVDRTCFWHGGM
                     ALYNNGFAAGLTATLIVSVIDWYRSSKVANKVE"
     gene            23697..25328
                     /gene="pruA"
                     /locus_tag="PK105_00105"
     CDS             23697..25328
                     /gene="pruA"
                     /locus_tag="PK105_00105"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOU60134.1"
                     /translation="MNNGIFHIPRPINEPVFNYAPGSLERATLKKELDHLRTNQIEIP
                     LIIGGRELRTGKTATIRRPDAHGEVLGVYHLAGETEVKMAMEAALAAQEGWAAMPWEE
                     RAAVFLKVAELISGKYRALMDAATMLCQSKTAYQAEIDAVCEAIDFLRFNPWFMEQIY
                     SQQPDNVKGEWNRSAYRPLEGFVYAVTPFNFTSIAANLPTSPAMMGNTVVWKPASNAV
                     YSNYMLMRLYMEAGLPPGVINFVPGSGSLISNVVLASPELAGFHFTGSTEVFRSLWKQ
                     ISSNLEYYRSYPRIVGETGGKDFIFIDPSAELEPTVASTLRGAYEYQGQKCSAASRLY
                     IPESRASEFIEALVAQISTIPMGEITDFRNFMGAVIDETAFENIHGYIERAKAQGAKV
                     LAGSNCNKSHGWFIEPTLILTEDPRSETMVNEIFGPVLTAYVYDDRDMEAAYRLIDST
                     SPYGLTGAIMATDRERINRALLAFQNSAGNLYINDKPTGAVVGRQPFGGARASGTNDK
                     AGSIINLLRWVSALAIKENFSPPADWRYPFMKGDD"
     gene            complement(25547..26347)
                     /locus_tag="PK105_00110"
     CDS             complement(25547..26347)
                     /locus_tag="PK105_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOU60135.1"
                     /translation="MNIENHVMQTFFATLFTWGMTALGAAMVFFFKEINKKVLDSMLG
                     FAAGVMIAASFWSLLSPAIELSESMGLIPWLPAVIGFLLGGAFLLGVDHLLPHLHIGA
                     DMSEAEGLHTSWQRSVLLVLAITLHNIPEGLAVGVGFGAIAADIPSASLSGAIALAIG
                     IGLQNFPEGAAVSIPLRREGLSRRKSFVYGQASGLVEPIAGILGAVLVLAMRPILPYA
                     LSFAAGAMIYVVAEEVLPEASRDSNGHLATIGTMCGFALMMLLDVALG"
     gene            26641..27708
                     /locus_tag="PK105_00115"
     CDS             26641..27708
                     /locus_tag="PK105_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU60136.1"
                     /translation="MSHYKNLVFKGGGVRGIAYIGALQYLYERGIMPHIRRVAGTSAG
                     AITALGVAMNPTDFMGFRTLADSLDYRKVPSEQDENTEKLTSKRLIAIIEHYRELAIF
                     KNLQCSIRLLQEKGWYSSEYLYTWLRSIIAEYFTVAKEIYTFADFANTQIHRDGRPFL
                     DLYITGTDVSNRTARLFSLETTPDMEVALAVRISMSIPLFFEAIQYQYPGTEEPQFYV
                     DGGVMWNYPINLFDDHKYCRKLNDGANAETLGLFLFSSSEKTHYPPIKSMLDYMRSLF
                     ESVSTVQEQLAIRTEKNYSRTIYIDDCGIEATDFDIQPGDERHRMLIDSGFRATREFF
                     ESRTEWSQFLAFLRERFGWKE"
     gene            27838..28626
                     /locus_tag="PK105_00120"
     CDS             27838..28626
                     /locus_tag="PK105_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOU60137.1"
                     /translation="MDLVPEKKPNPAIKQVIGVISGKGGVGKSMVASLLAQSFAARGL
                     KVGMLDADITGPSIPRLLGIDCFRGESDGENLYPVQNEEGIKVLSINLFNENEDDPVI
                     WRGPLLAKVIDQFWSDTIWGDLDYLIIDFPPGTSDVALTAFQTIPFSGLVVVATPQDY
                     VTMIVRKSVNMASMLKTPVLGVVENMRTMVCPHCGAEIALFDDGSQNGAKRMELPLLA
                     SLPWRKELAQARSLRWSALSQEIKRDADRLANEVEFALTAFSPK"
     gene            28792..29958
                     /locus_tag="PK105_00125"
     CDS             28792..29958
                     /locus_tag="PK105_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOU60138.1"
                     /translation="MKGKRLLMIPGPIEFTLEVLAEMAKPTLSHTDQQFIEEFGQALE
                     KMRKVWLAPNGQPFAIAGSGTLAMEIAAANITEPGDNVLIVHTGYFSERMADIFRLHG
                     ANVDIVQSAVGDIPEIDEVRRRLKSKNYKILSITHVDTSTGVRTDVKALSAAAQETNT
                     LVVVDGVCAVAGEELRMEEWGVDIAFTASQKAIGVPPGLALLVTSKKAMESFRSRKSP
                     VGAYYCDWQYWLPVMEAYEGRKSAYFGTPAVNLVRALNISLGQILDEGMEARFERHAR
                     NAAAFRAGLSTIGLSFVPVRKEIMANTLSAVYYPEGTDASILPLIAKNGAVLAGGLHP
                     AIKTRYFRIGHMGMSDASEILAVLGSIEQGLATAGYSFEPGASLKAAQSALIQP"
     gene            30080..30925
                     /locus_tag="PK105_00130"
     CDS             30080..30925
                     /locus_tag="PK105_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60139.1"
                     /translation="MWNFFVPKESQWYCWKLDGASLWMRKNGEEWRFAASSIPFKQIQ
                     NDSAGPLFVDAPSELSIAFAVAPGKKAALRPHPSTAPYLVSARNDVKIYPGTEAQFDI
                     ALPPIFRIELENGLSLFESLPFVTPYTWFGDKVSGDLCLSLPIELDPQCKNEQGYFER
                     IDSNNPISLDPIMQESLQRAAQYLSCRSLIHCKIVVKNRSKEPLNVKRLAIFTSLMNV
                     YQKQDNLVSDTVIITAAADDSLQTNIDDSAYKNLPKIHAAPKTGLNELLVMKGVSFLR
                     SIAGL"
     gene            30939..31805
                     /locus_tag="PK105_00135"
     CDS             30939..31805
                     /locus_tag="PK105_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOU60140.1"
                     /translation="MDDTIFPDSIDRFIQDALTPEFWSKAFTILITAILILVFFRILR
                     IILGRGMKKTIPEQKAQLILKALRYTGWAIAVVSVLQSVGVNLSALLGAAGIAGIAIG
                     FAAQTSVSNLISGIFLVFEKPFQISDTIQIDEISGIVMSIDLLSVKLQTFDNRFVRIP
                     NESIIKTNVINLTRFPIRRLDIYVNVSYTTDLRKATDVLRDIAYKNIYVLDNPSPLIL
                     IDKFDIPGVGILLGVWFEKSNLVEIKNTILMDIQERFAEEGIEIPYPKRDVYIKGIAE
                     RNLIEIDRKENT"
     gene            complement(31853..33286)
                     /locus_tag="PK105_00140"
     CDS             complement(31853..33286)
                     /locus_tag="PK105_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOU60141.1"
                     /translation="MVIQDKEQLDKLFESQAERRWKLALTGPEERIVKLKKLRSAILS
                     RSDELYAALNSDFGKPAFEAWLTEVFPCIEEIDVAVRHLKVWMKGRRAKGTLILPFSS
                     TRVRYEPKGRVLIMAPWNYPFQLLIAPLVSAVAAGNCVIAKPSNKTPHTAAFIADLIA
                     GVFDSQEVAIVEGSGSTLGDFLLELTFDHIFFTGSPAVGVKVGQAAARMHAGLTLELG
                     GKSPAILLRGANLKQASKRIAWGKFLNAGQTCIAPDYLFCPMELVGEFSVEMRLAIES
                     FYGKSEEERQASPDYPRIVDKAACARLERIVSEAISKGAKLEFGGRFDVENRYVSPTL
                     LTGVQIDMAIMAEEIFGPILPVLAYDSLDEAIEFIRSRPKPLALYILGKDKKSAERLL
                     AETTSGSVGINDLIVQIENLDAPFGGVGMSGTGSYHGFYGFKTFSHERTVLYQGPISA
                     VEKFYPPYNTSTQKRLLGALKKIKNIS"
     gene            34196..37756
                     /gene="nifJ"
                     /locus_tag="PK105_00145"
     CDS             34196..37756
                     /gene="nifJ"
                     /locus_tag="PK105_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOU60142.1"
                     /translation="MPNNKNMVMIDGNAAAAHVAHACSDVIAIYPITPSSPMGELADE
                     FSSMGRNNIWGTVPQVVEMQSEAGAAGAVHGALTTGALSTTFTASQGLLLMIPNMFKI
                     AGEAIPTVFHIAARAIAAHALSIFGDHQDVMATRQTGWALLASNNVQEVMDLALIAHA
                     ATLKARVPFLHFFDGFRTSHEVSKIEELSYDIIRAMIPEEKVREHRARGLSPEHPVIR
                     GTSQNPDVYFQGRETINKLYEATPAIVEETMKQFAEMTGRAYKLFDYSGAPDAERVII
                     LMGSGADTAEETAEYLAKQGEKVGVIKVHLFRPFSVEHFIKAIPATAKSIAVLDRTKE
                     SGAIGEPLYEDVRTAIGEAMGNHTAPFKNWPIVVGGRYGLGSYEFTPAMVKAVFDNLK
                     LEHPKNHFTIGIDDDVSFTSLPYDEKFHLPGDGIIQCLFYGLGSDGTVGANKNSIKII
                     GDETENCAQGYFVYDSRKAGTYTISHLRFGPKQIKKPYLITKADFLACHKFSFIERID
                     ILSNIKEGGTFLLNSPYSADEVWDNLPIEVQKEIIDKKLSFYVIDAMAIADKAGMGTR
                     INTIMQTAFFKISGVLPEEQAVELIKKYTEKTYLRKGADVVQKNLDAIDLALNATHKV
                     KIGQADSIKHMLPPVPQTAPRFVRETLGEIIAQRGEKMPVSKLPLDGTFPTGTTQYEK
                     RNIAEKIPVWEPSLCTQCGNCSLVCPHGVIRMKAYDPAILENAPSTFKSTDAKGKEVA
                     GLKVTVQVAPEDCTGCGACINICPAIDRANPGRKAINFGPQIELRDTEKQNWDFFLSI
                     PETDSKFLNLSIPKGIAMKQPLFEFSGACAGCGETPYIKLVSQLFGDRAIIANATGCS
                     SIYGGNLPTTPYTKRADGRGPAWSNSLFEDAAEFGYGMRLTSDKQNEYARELLASAKG
                     KGIPEELVDRLLSNTQDSDEQIEAQRADVAALRESLAGKSESWAKELDNVAEALVRRS
                     VWIFGGDGWAYDIGFGGLDHVIASGKNINLLVLDTEVYSNTGGQASKATPIGAIARFA
                     TAGKETSKKDLGMIAVSYGYVYVAQIALGANMVQAIKAIREAESYNGPSIVIAYSHCI
                     NHGIDMMKGMDQQKMAVQSGMWPLYRYDPRLKAQGKNPFQLDSREPDTSKLERYMYAE
                     VRFKSLVQANPERAKVLLEKQRELIGRRYNEYRYLADRPF"
     gene            37883..38245
                     /locus_tag="PK105_00150"
     CDS             37883..38245
                     /locus_tag="PK105_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOU60143.1"
                     /translation="MNTIKSYKWTIASAMVVLLLLLMPFRIFQQTPQSLNDFDKFIHA
                     ILFGAMTAVFCAEHRALKKINPKFLLSLVIISAFAFLTEIGQLLTITRHFDLKDFGAD
                     AIGIAAALLVMRIATMLS"
     gene            38319..39128
                     /locus_tag="PK105_00155"
     CDS             38319..39128
                     /locus_tag="PK105_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU60144.1"
                     /translation="MKNLEKQGFPAPKNESTPASEKSAKIESLTLLQLQKSSFLFFLI
                     TSMFVVFCCILMILMPEIAFLTYYSPEMLILIGLFSFGFLIVHLNRKLAWLGPVLIIA
                     LLPLTLTRYLTFMYSICAFIIALIELNRLGFFKHAGLFKACILISYYAWNFALVAIMH
                     KEFFLDIILSIAFLSLFFIYLIPIIQDRSQIGKVDLAYRIYPRDLNLTCREMDFLRDC
                     LNGATFKEIAYKHHVSESTVRNTFAHIYHKFNVVDRIDLMSKLAAFDIIDE"
     gene            39145..40044
                     /gene="mazG"
                     /locus_tag="PK105_00160"
     CDS             39145..40044
                     /gene="mazG"
                     /locus_tag="PK105_00160"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOU60145.1"
                     /translation="MDLDSLALRNIAGENADTLLADADRTAKAFRYFYVVVSRLRASD
                     GCPWDLEQTPSSIRGNIIEEAYELAEAITEDDSLHIKEETGDLYLLATMVGYMCQQEG
                     RFSVADSLCDEARKLIRRHPHIFGESEVLSPDQVVRQWNEIKEQVEGRRKKESILDEV
                     HRSLPPLEKAYKLQKKAAKVGFDWQHERDVWNKLEEELGELMQARAWKSEECPSDIAT
                     DSGSSKDALEEEFGDLLFTVINAARLYGIDPSIALQRTNGKFSRRFRHVEESMKQQHL
                     DMNQENMDMMDAFWNEAKGAEHS"
     gene            40113..42341
                     /locus_tag="PK105_00165"
     CDS             40113..42341
                     /locus_tag="PK105_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU60146.1"
                     /translation="MAILLSAQKLAKARKTFNIYNFLNSFSFVFVSGSIITLFALKLG
                     AKNSIIGLLNALAYVTFFMLPLGKRLVKRSSIIKVFGWGWVIRYIALVPILFAPLFAS
                     KGHMGLAILILILGTAGFHISRGIALIGNNPVLGLLSTGGGDKPRSDQGRYLVNISVV
                     NSVAAILANIVLAFLLGQEASLWTYTIAIALGIITGLISCAILFKTPEPEAFSGKDEQ
                     SLWDVTKEALVQPDFRAFILTFMLLSFLSGMGRAFLPVYAKEVFAQGDDAIMVYSIVA
                     SLGSITMGLITRLVVDRLGPKPLFIIYSAIGFVSFLPIALIPGSATLMGAPSVIALFL
                     AIVHFLSSFGFAGEENAAQVYYFGLVPKEKMLDLAVIYNLAYGIGGSLGSFLGGGILE
                     VFANMELSDAGAFRLYYAAISVGLVIVIYSMRNLPHMGAKSVTQSISILLSPRELRAF
                     DLLTRLDQSASAHEEVRIIQKLSKSESNLTQKELVEYLYSPRFEVRSEALLALESIPE
                     LDHETIVALIREVKANVYTTAYVAARILGKQRSRIAIPALIESLDGPDYMLQSMAMIA
                     LARLQAFEAVGKIEQLMRSMENPRVRISGAYALELFGRIDSIPTLISCLKAEDRPASV
                     SDELVLSISGILGIDKEFYLLYSEFIDDNSYGIAQLIAYSKDLNLGDDRLNVWISAVD
                     ALFAPDSDTAPLARLLLASPRITNSILMLSDGLLDPGLTYPGFNFLCAYIALHIEDLK
                     DY"
     assembly_gap    42353..42452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(42488..43081)
                     /locus_tag="PK105_00170"
     CDS             complement(42488..43081)
                     /locus_tag="PK105_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOU60147.1"
                     /translation="MVFSTLAKIVSSLVSLYMILCIVRIFFSWIPSLTDTKWGRLIAG
                     LTDPYLNLFRGFSLFRTPAIDFSPIVALAVLSVLNNLFMTLSYAVRITLGFILSLLLD
                     AAWSAISFLLGFFLVISLIRIIGFLARIAVLHPLWQILDGIINPLLFRINRIIYRGRT
                     VQYLQGLITGFIVVLVMRTLGGFLVRLLTSLLMSLPI"
     gene            43143..43934
                     /locus_tag="PK105_00175"
     CDS             43143..43934
                     /locus_tag="PK105_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOU60148.1"
                     /translation="MHIGNVHIGTCGFSYEEWKTVFYPADLPSNEMLRYYSLVFSFVE
                     LDNTWYRMPNPAQLKRLALLTPSDFRISLKVHRSLTHEIDESWQTKANEFSLAVSALS
                     STGRLGCVLVQLPYRFSYTIENRKYLANLLAALSDFPLVVEFRNATWYQDRVFETLRK
                     LKVGLVTVDRPDLPGLPPESIIITSDICYYRLHGRNTDLWWSGNAKSRYEYCYSEQEL
                     KDRARLIRIMAKQSKTAFVAFNNHSNGYAPKNAAYLQSILAGQQL"
     gene            complement(43948..44388)
                     /locus_tag="PK105_00180"
     CDS             complement(43948..44388)
                     /locus_tag="PK105_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60149.1"
                     /translation="MRKPFEISTGAWWVVADGRTIAVPSFHESWLASHPGIASGALHT
                     IDFVEKSGWLSVTLYSEGLIEVISRDILDNRQREALRQLLETNRSIIRKMVLFVPSID
                     GCFTAEDLLLDDWERLWKEIETFAAKEIRQNLSEEGMEVDTGQP"
     gene            complement(44372..45190)
                     /locus_tag="PK105_00185"
     CDS             complement(44372..45190)
                     /locus_tag="PK105_00185"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU60150.1"
                     /translation="MIRILLENDEFIVIDKPAGLAAQPGEGLRDDVITALERQFGYRL
                     FPVHRLDKDTAGCMMLAKDARAANKWSNRIASREITKRYQAWVAGLPNQEKGTIEDAL
                     HVKRGIQAACTLWTLRETWLFPLQEHSNRRADVQDGGHVHNNRIGVDRYEANSSIAAA
                     EANFLTLSLLDLELSTGRMHQIRRHLAGIGLPIICDDKYGDFSLNKALRKNGIRRLML
                     WAYELVLPLPSLESPNLPLFAAEPAHFISLRQTLEREGRILDKRNTEQSDEKTI"
     gene            complement(45318..46166)
                     /locus_tag="PK105_00190"
     CDS             complement(45318..46166)
                     /locus_tag="PK105_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOU60151.1"
                     /translation="MNQGALETIDAAIPNLAEAERKIAAFVQEAPQKALHLNISELAR
                     QAGTSPAAVVRFCRHIGVGKYSDFKLWLAKDVYQGWGEKFLPDLELESQTPGARAIRG
                     MTEAVRRTMNSLAQTLDPRAVEEAAERIKGASMTAIFGIGASGLVAEDFHQKLTRIGI
                     PSSYLFDTHAQITASCSLRQSDLAFIISYSGETNEMIEVAAQSKARGCFLISMTMTGQ
                     NRLRDYSDLILEIPTVERIYRSAAELSRISQLAAIDILFNVLISYDLDSAISSLERSM
                     QATHHF"
     gene            complement(46123..47127)
                     /gene="murQ"
                     /locus_tag="PK105_00195"
     CDS             complement(46123..47127)
                     /gene="murQ"
                     /locus_tag="PK105_00195"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOU60152.1"
                     /translation="MDIDSVDEYLERATTESRNVRSEHIDALPVIEVLRIINEEDKGV
                     PFAVEKALPSIAALVEDVVKAFNSGGRLVYIGAGTSGRLGVLDASECPPTFGVPPDMV
                     VGLIAGGEAALRNSIEGAEDHPENGEKALRDINFSEQDVLVGITASGAAPYVLGAMEY
                     ARNLGAVVAAISCNRQSRTFDIARHKILLEVGPEVITGSTRMKAGTAQKLVLNMITTS
                     SMIRIGKVYGNLMVDLMPVNRKLIGRSKRLIRQATGCSKDEAEAAFLASGGKPKIAIL
                     MTILGIGAEQAAELIEHGEGRLSEAIRIYSESKGDSKQENKGERNESRSARNNRRGNS
                     "
     gene            47312..48556
                     /locus_tag="PK105_00200"
     CDS             47312..48556
                     /locus_tag="PK105_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU60153.1"
                     /translation="MAKKLSIVLLLIAILSPMYAQQKTVSIWGWRAQDVDVWKGVETA
                     LKAKGEQISIRYEVFPPTEYDSKLLVSLQGGVGPDIMYTRRLPGARTQALLDNGYLTA
                     INGLVDLSNFDDVSLSFISTDNKVWGVPFANQIIGIFYNRAIFDKYKLKEPQTWDEMV
                     QIAETLKKNGVTPFFVTGKEAWILAMQNAMVGVSYPGDEWIGKLTVGKAKFTDPEYVN
                     MLKDLNALKKYYQKDFMANTSAEQDVAFAMEQAAMVFYGVWGNTNWLKMNPDLKFDYF
                     PIPPKDKKVPAKAYTYMDGAYGLNNTSKNKEAALKVLNYTGTKAYGELFSSTTGEITA
                     INGVTMPASKPILVKCYEKMTTIAAKNRYWVGSPFDAGTPSVYNILEKDMQSMYLDQM
                     TPEALAKRLQDGVSTWYPAFKK"
     gene            48661..49560
                     /locus_tag="PK105_00205"
     CDS             48661..49560
                     /locus_tag="PK105_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU60154.1"
                     /translation="MRKYSFLWMLLPAFILYSLFIAYPFFSSLGLSFYEWPGIGPKKF
                     IGLANYKNILTGFMSSEFYNALWHNVVFFIWSLILSVVPGLFFAFLLSANIKGTGWLK
                     VVFFFPNTLAIVVVGFLWGLLLNPQWGLINQLFRLVGLDFLAKPWLGDTKTALPTIIF
                     VTAWRGLGFYILIYLAAILGVDREMIEAARIDGASELQIAGRIILPHLYPIIATTSML
                     KFIWTFNIFDIVYAMEGTQGGPAGSTDVLGTLFYRIAFGGLGASQVGMGLGATVVTLI
                     FLIVLPVSIFYVFIVEKRVEKGQ"
     gene            49557..50387
                     /locus_tag="PK105_00210"
     CDS             49557..50387
                     /locus_tag="PK105_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU60155.1"
                     /translation="MMFKFNGFQKALAWIFLLLLAFANLYPIGIMMLSSFKSTKEIFL
                     EPFSLPSVWRWQNYVSAWQRADFATYFKNSVLVTLMSIVSIIIVSSMAAYVIARFDFK
                     FKRGIYLYMLAGLALPTRLAIIPIFLIMRNLHLLDKLSGLVVVYTASGIAFSVFLLVN
                     FFKNLPHDIEDSAKMDGAGPFRIYWQIDLPLLKPALVTVAMFNFIDVWNDFFFPLILL
                     SSKAKKTVPLGLQAFFGEYTVEWDVLFAALNISVLPILIVFLILSKQFIAGLTEGALK
                     "
     gene            complement(50456..51640)
                     /locus_tag="PK105_00215"
     CDS             complement(50456..51640)
                     /locus_tag="PK105_00215"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOU60156.1"
                     /translation="MSKSKQQRASVVLGLENFLDDTRRYNKRYGLATNSAAITSRGIP
                     SWKAFIEHGIIPACLFGPEHGFRGAAQDAVGLDDEVFRGIPAYSLYGKRLRPEPWMLE
                     SLDALVFDMQDVGCRYYTYLYTLAYIMEVCEKVGTPLIVLDRPNPIGGSKIEGSPISK
                     EAESFVGGYGLPPRYGMTIGEFALYLKGEYYPNCELEIVKMSGWNSRIAWEGTNLPWP
                     LPSPNLPTLSCAELYPGTCLVEGTWLSEGRGTSRPFEIIGAPFIDGEALREQLAAFEL
                     PGVVFISLFFSPSASKYKGELCEGVLVSLTDRQALRSLDTGITLVHTIHRMYPDKFRW
                     REDWDDKSLSFFDKLAGGATLRYMITSGASLEDCLEFAHRGESQFLRIREKYLIYGAQ
                     KG"
     gene            51664..52848
                     /locus_tag="PK105_00220"
     CDS             51664..52848
                     /locus_tag="PK105_00220"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU60157.1"
                     /translation="MTYNKNKGSSHRHNPTEESLENLIKDALSKSLCTGIAAAVSMDG
                     KLFWSHCSGFTAREEPAAAQSIENHDQASKVVIAPIDAETRFDVASMTKPLSTSLLIL
                     KALQAGALDLKDPLGKYLPELNYKTGQIPILALLTHTSGLPAIPALHSLFPDSERLDR
                     NRAFEKLFSIEPEFAIGRHVVYSCTGYIFLGVLLERISGMLLGELFQKEIGTPLGLGQ
                     ASFAESIGTSGEPKPLGNAVATEYCPWRHRRIQGQVHDESSYCLGGHSGNAGLFLSLN
                     DAMAMGNFIMQEGTFDGNQILKPEIIQLLWREYTPGLEERRSLGFKLHDSTTIDGPLW
                     PPQSFGHTGFVGTSIFFEPQRRIFSILLTNRVYFGREQTTDSIFAFRREFHSRVWRAF
                     AE"
     gene            53169..54473
                     /locus_tag="PK105_00225"
     CDS             53169..54473
                     /locus_tag="PK105_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU60158.1"
                     /translation="MKRAIALFVLAIAIIASVPAQAPVVPGLGNKKITINLWTHEDPN
                     RSIIEKKYIAEFMAANPNITVNYNTYPSNRIFEALTTAFAGKQGPEIFNVSIGNAVSF
                     IDNGRVSPVDYKALGLKGPVDLEAMYLPGMLNAVERDGDYYGLPLEITNMCLYVNRKM
                     LKEVGIDPDKEWPKTWEDLISLSEKIVKREGQIITRRGFDFRYDAYTNLFIPMVEQLG
                     GEYVSKDGKKAIIGDEAWLKALQWMKELGPAGKNLGSPTYTAARTAFDKNDGTVAMSF
                     SGLYQEARMKASNPNFYNSKDWMVVPFPQWKDSKKKVTANYSAHYYMVNAESDKLVQQ
                     ASWMLVGWMLSHGEEYLEKVAIIQPTKKLIESPIYKSMPYSDVFAKDFENAKIVYVGK
                     ASARLSTLLSETIQNVMVGGVDPKVALEKLRKSAQEALDSAD"
BASE COUNT        14739 a        13196 c        12384 g        14268 t
ORIGIN      
        1 atatttctta cctgccgatc gatcgcttgc agccaaaaac tgtggataga accatggatg
       61 attggcaatt ctgcgtagaa aacgctgccc cactacacct gttgcaccaa gtattgtgac
      121 tggaatcttt ctagaaggag aaaattgcgg ttgagattga ttcttattca tggtacctcc
      181 ataccttccg gtggaggccc gcctgtgccc ttcaaagaaa tactattcct gaaagaagct
      241 ccagagatgc cttcaagcta agcagctata cctgagctcc tttcgttaag aaaggcaaac
      301 gtcaggcgct ccgcggaatg atctatttcc gcgacagccg ttgggtatta gcccagacgt
      361 ccaagaacag ccttgagaga aaggctttgt cccttcggcg atcctcccct ttctggtgcg
      421 ttcacaggct ctctctgcct ctcgtccata ctcatgggga tcgctcctcc atcggtgaaa
      481 tgaccctacc atagagtctt aggttgctac aagggggtag attggctctt ttcgaaagaa
      541 gtctaagctt agattcaacc cccatcttgc attaaaggct caaactcgct attcctatct
      601 gcaaacgagc tcttatgata cagtcatgtt attcttcttt ttctgagcta gaagtcgctg
      661 cctcagatgc tcttcttgag ctctctagga tgttactcag gattggagag ccagaggaag
      721 tcttgggcta ttttctcaat tccattctgc ctctattcca tggacgggct tatgctgcct
      781 gctatcgggc ggattccgga gatgttctac gatatttcca agttggtccc aatgaagatt
      841 cctggtttga tgaaaatgtt aggatcggcg aggaagtctg ccttggttct gtgccctgtg
      901 gtctagccct cgaatccgat aaattcgttc agaaaaaatt gaatagaagt ttgattcttg
      961 agggtcccat aaagcaaaga gataatacta tattcgcgat aagtattttg ctttccgggg
     1021 attgcgcttc cgagatcgta tcgcttgcta gcgagaccat aaagagggcg gcaggaataa
     1081 ttgaagaatt tcttggaatg cgatccaggc ttgcggaaaa aatcgataga gataatggcc
     1141 ggctcattgg tgttatcgat tccgatctaa gcgaatctcc ggtgtcttgg tatgggatag
     1201 ttggcaactc tgatgtgatg cgagaaatgt tttcgatgat cgatcaggtc gcggacactg
     1261 aagcaacggt tctccttctc ggagagagcg gcactggtaa ggaattgatc gcacaggcca
     1321 ttcatcagag aagctctcgc gcctcaaagc ctttcgttgc ggtgaactgt gcggctcttc
     1381 ccgaatctgt aatcgagagt gagctttttg ggcacgaaaa aggagcttat acgggcgcgt
     1441 tcgagcagcg gaaaggtcga tttgagcagg caaacaaggg aacgcttttt ttggacgaaa
     1501 ttggcgaact ctcgacggct ctgcagatca agcttctgcg ctttttgcag gaccaccgct
     1561 ttgagcgtgt tggtgggaat gaaaatatcg aagcagatgt gcgggtaatt gcagccacaa
     1621 atcgcaatct tcagcaagcc gtagacgagg gttcttttag agccgatcta tactataggc
     1681 taaatgtatt tccgatcaat gtccctcctt tacgcgagag aggagcggat atactcctct
     1741 tggccgacca ctttgtatcg cagttcaacc aagaaaacag caaaaagatt cagcgcattt
     1801 ctacgcctgc attggatctc ctcatgattt atcattggcc cgggaatgta cgagagcttg
     1861 aaaactgcat tttgcgcgct gcgatccttt cgacagatgg tgtgatccac gcctaccatc
     1921 tacccccaag tctccagtct gcagcttcca cagggacaga gcccaaaaca ggtctcgatg
     1981 cagagatctt tagggtcgaa aaggagctga taatagaagc attaaagttg gagaatggta
     2041 atgtttccgc tgcggcacgg aggcttgtaa tcaccgagcg ccggttacga tatgcaattc
     2101 agcgccacag gatagatccc aggcgtttta agacaaaatt gtaggccttt atccaatttt
     2161 acaaatttgt aactttttgg ctacccgaaa tcgtatatca gaatttttaa gagaagggga
     2221 gaccttttcg cacacagtgc tcttttatgc ctttttatgc atacctgaat acggaatagc
     2281 ataatttgcc tttctattcg atattcataa tagtctatga aaaaagaaac tagaatactt
     2341 ttcatgaaag atcttcattg tatcggcccg gcggcaatat tcaagaaagt gatgagaagg
     2401 aattattgat tactttttgg cagaagtgtc gctgtttggc acaattcttg ctactttatt
     2461 caaaatatat cttgtctttg ctggagaact cattatggat gagcattcct cgaatttttc
     2521 attgatcgat cactttggct ctcgctgttt ctcaaatgat gttatgcgag agcgcctgcc
     2581 tcgctcggca tttgaggcaa tactcgaaat tcaggctggc aagcgtgagc tcacgcttga
     2641 tattgctgaa atcgttgctg ccgcgatgaa agaatgggct tctgaccttg gcgcaactca
     2701 ttacacgcat tggtttcacc cattgtcggg cttgaccgca gaaaagcacg aatcctttgt
     2761 ttcaccacag cctaacggca gcattctcat ggaattctcg gggaaagatc ttgtcaaagg
     2821 cgagccggat gcctcgagtt tcgcgtcagg cggcttaaga gcaacctttg aagctcgcgg
     2881 ctataccgca tgggatgtga caagccctgc ctttctcaag cgcaatgccg aacgatacat
     2941 tctctgcatt cccactgcct ttgtgtccta taccgggctc gcactcgaca agaaagtccc
     3001 ccttttgcgc tccatggaag ctctgaatcg tgctggcttg cgtctattgc gccttatcgg
     3061 agatagcaaa acagagcgta ttatagccta cgcaggtcca gaacaagagt atttcctcgt
     3121 caatgccgag ctgtacgcaa agcgccccga ccttaagctc tgcggtcgaa ccgtacttgg
     3181 tactatggcc gcaaaaggtc aggaactcga agaccactat tatggcgcta tagatgaaaa
     3241 agccgctgcc tttatgaacg aactcaacag cgagctatgg gctatgggta tttccgctaa
     3301 gacccagcat aacgaggtgg ctcccaatca attcgaaatc gcctgtatct actcacagga
     3361 aaacatcgct gccgatgcta atcagcttgt tatggagaca atccgaaaga ccgcggaaca
     3421 ccatgggatg gccgcactcc tccatgaaaa gccattcgcg ggcataaatg gttccggcaa
     3481 acatatcaac tggtctattg gaacagatga gggattgaat cttctagatc cggcaattgt
     3541 cgcaggtatt tcatccgaag aagaggcagg gttcaaattc atacgattcg cgcttttttc
     3601 tcttgcggtg atagaggctg ttgataggcg cgcggggctt ttgcgttcct ctaccgctac
     3661 cgcgggcaat gaccgaagac ttggcggcca cgaggctcca cccgcgattc tttcgatata
     3721 ccttggaaaa ccactctacg acctgttcaa ttccctgcaa aaccaaaata atgcgcagag
     3781 aatcactaat gcctttttgg aacttggtat aagaagtctt ccaaagctcc cccgtgattt
     3841 ttccgacaga aaccgcacaa gcccattcgc ctttaccggt aacaaattcg aatttcgcac
     3901 cgtgggttct tctcaatcag ccgcaacttc gctcactatg ataaatgccg cggttgcaga
     3961 agcattgaat cgcatttccg aagagatcga acaagagctc acaaaaggag cacatttact
     4021 cgatgccgcg cgccttgttg cacaaagatc ctggcaagag caccgccgcg tcgtattcaa
     4081 tggcaatgga tattctatcg actggatgca tgaggcagaa aaaagaggat tgccggtttt
     4141 tcgctccgcg gtcgatgcta tccaggaact aagcacaccg gacaatgtaa aaatgctgga
     4201 agatctatgt attcttctgc cagaagaaat cgaggctcgg cagcatgttt atctcgagcg
     4261 ttactccaag cagataaaaa tcgaggcggc catgatgatc gaccttgtcc gcagatacat
     4321 cattccagcg gcgagcgagg cagcgagcaa ctacggttcc atatgctcaa gcatcgccgc
     4381 cataggagct ccggctatcg tgcaagaagc tcttgctaaa cgcatagccc aacttgttgg
     4441 caaatcggcc gaagggcttg atcgcctgga aagggctctc agtgatgcaa gttgcacaga
     4501 agatgaactc gagcgagcaa agtcttaccg agacatggtc gtgcctagca tggatgaact
     4561 cagggaattt tgcgacgaac tggaacgtct ggtgcccaaa tatctttggc ctctacccac
     4621 ctatagcgat ttactgttta ctctctgaat caagcatccg catcttttgt aaaactactg
     4681 agcatcgcgg ttcgaaagac aagagccttg aacaggatta catagaacaa tatgcctatg
     4741 ttgccctcct tgcgcgctcc tgttacgata gtgactgatg aagtttctgc gccgaattat
     4801 tccatggatt cttgtcgcta cggtcattct tgccgccctc tacatacctg accttgggcc
     4861 tgaaaattac agagatttcg attcgctttt tagaaacgaa cgagtgcgac agggaatagc
     4921 cggctatgag gtcgtaatca tcaagggtgg agaaatcgtc ttcaataaag cctatggctt
     4981 tgatgggcaa cgaaatcctt tggatatctc gactcctcta tatatgggac ctgcctcaga
     5041 gatctttacg gggacattac ttgcccagct tgtaaatgaa aaaaagataa ccctcgatgt
     5101 gccaattact catttcattc cggaactcgg ttctctaaag gccaggacag cgagcgacga
     5161 ttctctcgat cttccgctta ctctgcggct tcttgcagca catcgtgtcg cgtttccgga
     5221 aaaagacctt ccggaattcg atcctgaaac catgggtctc gaggctggtc ttccagacgc
     5281 agatttattt ttgagttctc attttcctac ggagaaatat acgcggagtc ggctttctta
     5341 ccggattgtg ggcacaatac ttgaaaaggc ttcgggcatg aaatactcag aactcctcga
     5401 atcgaagata gctgttccgc tggatatgag atctactact gcagagccct cctcgatgct
     5461 gagtatcgcc gtaggtgccg gttcgttctt tggcctgaca tttccttatg tcgagaaagt
     5521 tccctatgat gcagcgccct ccgatggcat agtgactaca agtgaagatc ttgcaaaatt
     5581 cttgagattc gtcgtatcgc caaagcgtgg ccagagaatt cctgggcttt cttcagcgca
     5641 gacacccatg ctctatcagc ccctctacaa ggatggtgat actggttttg gatggagaat
     5701 catcgagaat aaagaaggcc gatccatttt tcaagggggc tcgattcgag gctatgcatc
     5761 gcgtattgtc atctatccgg agcgcaatgc ggcaatagcg attctctgtc cccaggatgg
     5821 tgtgctcata tccaattttc tgctcccaaa gctcgtctct tgtgcggaac aaatcttgtt
     5881 cgaaggagag agcggtcgac ctttccctac acagcgtgct cagctcgtac tcggcttcat
     5941 cttcattatt tatttgctaa acatcatctt gcaaacgctt gtgtcttatt cctgggccag
     6001 gaacctgctc aaatatcgcg agacaggtat atcccaactt tatgcccgct ttgttatttt
     6061 gagaaccatt attggtttgg tgctgcgcat tctcatagtg ctcctggctc ccctagccat
     6121 cggagccctt gtgggatggc aggtgactta ccaagaaatc atagccttcg agcctggagt
     6181 atcgacaatg cttatgacag ctctattgtt tggtgctttg cgcaatatcg ccaggcttgt
     6241 aatggtctta aggctcaggc atttctgata tttccgaaat ggattagctc ctagaatccg
     6301 ctctattact gtgcttcgcc cagataggcc tttcgaacac gatcgtcttg aagtagctca
     6361 tgccctgtac caaaaagtga tatgcggccg gtctctaaga catatccata gtcagctact
     6421 tcaagggctg cctttgcatt ctgctctaca agcaataccg tcgtgccttc tgaatttatc
     6481 tgctttatta tctcgaaaat catcttggag atgaggggag ccaggccgag agaaggttca
     6541 tccatcatga gcagtttggg tctcgacata agccctcgac ccaccgcaag catctgctgt
     6601 tctccgcccg agagcgttcc acctttctgc cctcttctct ccttaagtct tgggaaaagc
     6661 tcatatacat gctcgagatc ggccaacaca gccttcttgt ccgttctgga ataggcacca
     6721 agaagcagat tctcttccac cgaaagattc gagaatattc tcctgccttc cggtaccagc
     6781 gcgaggcctt tctgaacgat ctctaaggtt ggccttccgg atataaccga tccctggagg
     6841 atcacctcgc ctgaacttgg cttgattagc ccggcaatgg cacgcagtgt ggtacttttt
     6901 ccagcaccgt tggccccgat aagcgtaaca atctttctat cgggaacatc gaggtcgatt
     6961 ccctggagcg catggatacc accgtaaaaa accgacagat tcttgatttc cagcatcagt
     7021 ccacccctag ataggcttcg atgaccttcg gattcttctg aatggaagat gggttgcctt
     7081 gagcaatagt aatgccataa tccagcacat acatgcgttc acacacgccc ataaccacct
     7141 gcatatggtg ctcgatcaaa aatatggtta gatcgaattc gtcccgaatc ttgcggataa
     7201 aatccattag ctcgaacgat tcctggggat tcatacccgc agcaggctca tcgaggagaa
     7261 gaatcttggg ttttgtggca agcgctcgcg caatttccag ccgtctttgc tgcccatacg
     7321 gaagacttgt tcctttttca tttgccaaat gtgccaatcc tacttgttcc agcaactcta
     7381 ctgaaaagcg cctggcttcg gactcgcgag ttcgatatcc aggcaagtgc agggttgcgg
     7441 ctagtattcc ggtatttatg ctcagatcgc aaccgaccag cacattctct agcacggaca
     7501 tttccttaaa tagtcgtata ttctgaaagg tccgcgctat accgagagcc gttatactgt
     7561 ggggcttaag ccccgtaata tctttccctt taaaaaggac ttttccttcg ctcggaacca
     7621 gcatgccggt tattacattg aatgccgttg tttttccggc accgtttggg cctatcaatc
     7681 caacgatctc gttctttctg atttccagat tgaaatcgct tacggcaaca aggccaccaa
     7741 atctcatggt gagatgggaa gtgctcagca taagctcagg gagttctggc atggctgcct
     7801 cccttcttgc tttttttgac aaatgcactc tggaacatat cccagctgaa ttccttattg
     7861 cccatgagcc ccctctggcg atagataatc ataaccataa ggagaataga aaatactacc
     7921 atgcgcagac ccggcatggc tggaatctca aaaaatatga ggtttatcgg ttcatctagg
     7981 aatcgcagtg cctccatcaa taccgttact atgatcgccg atatcaccga acccgatata
     8041 gagccgactc ctccaagcac cactatgagc agaatattgt aagtcagtgt aaaagtgaac
     8101 attttcggat ctatagtagt tatcatgtga ccaagtagag ctccaccaat accagctaaa
     8161 aagcttgata cggtaaatga agtgaccttt acccgaaata cattgattcc catcgcttcc
     8221 gcggcaagct catcgtcccg caccgctttc attgctctgc catagctcga gcgcatcaaa
     8281 agaaccataa aaagcactac aagacctgcc gctgcccata ccgcaaaggt ggtagaaaag
     8341 cggggcagac ccttcagccc ctgagctccg ttagttatcg actgggcatt tgtaatgatg
     8401 actctgatta tttcgccaaa accaagcgtt gcgattgcca gataatcgtc tctaagccgc
     8461 aggacaggag caccaataag aaagccaacg caagccgata caagtccagc tattatgagc
     8521 gcgggcaaga atggaatcgt aacatgttga agccatggca cgataggcaa aaggaaataa
     8581 ttcatctctt taagcggcgc aggcattgtg agaagcgcgc tcacataggc tcccaccgcc
     8641 ataaatccgg catgcccaag agaaaaaagc ccggtaaacc catttaggag attaagagaa
     8701 agagccagca caatgtagat cgcacataga ttgaatacgc gaagcgagaa tgcatcgaag
     8761 agtctgtcgg caaggaatat gaagagagca catgctccaa gaaaaatgat agtatagagc
     8821 atgtttttgg aaattgcccc gttttcgatg tgcctatctt tgagaatatg tctagtggaa
     8881 cttttcttac tcatggttat accttctcag cctggcgctt gcctaagagc cccgtcggtt
     8941 ttagtaatag gacaattatg agcagaataa atgcaaaagc atccctatac ccggaaagag
     9001 ttggcaataa tgcaaccatc attatttcga taataccgag caaaaatcca cctagcacgg
     9061 cacccgttat gttgccaatg ccacctatga ccgcggcaat aaaacacttt aagcctggca
     9121 taactcccat aagcggattt agctgaggat atttgaatga ccacatgatg ccgccaaaag
     9181 cagcaagcaa agagccgatg ccaaaagtga cagagactac cttatttaca tcgactgcca
     9241 tgagacgcgc cgcttcgata tctgtagaga cggcccgcat tgccatgccg atcttggttt
     9301 tatttacaat ccataagagc aagatcaaaa gcgcaaaggt aaagagggga ataattaagc
     9361 tgattgtaat catcgagaca gagccaaaat agatgacttt cgagagtaca tctggaacag
     9421 gcattccctt tggtcttcct ccaaaaacca aaactgcaag actctccaga agaaaagatg
     9481 cccctattgc gcttatcatg atggaaattc gcggcgaatc ccgaagcggc ttataggcca
     9541 ctcgctcgag gccgataccg aaaaatgcgg taaagataat cgacaaaagc agggctgccc
     9601 accatggcag agacaaaagc gtgatcccat agaaagcaac ataagcgcca accatgaaaa
     9661 tatcgccatg tgcgaagtta atgagcctca atatcccata caccattgtg tagccaatgg
     9721 cgaggagtgc atagaggctt ccgagcgaga gcgcattgac gatatgctga aataataatg
     9781 aaagtgtcat gcttcctcgt agttataaga aaaggagcgg atgcagtcaa cctcattcat
     9841 ccatgactct ccgctccttc tattgactta ttatatatcg gaaggagggc gcctttatgc
     9901 gcccaccatc ccacctccgg tgaaaatcct atgcgctatc agtacggttc aaccgtcgcc
     9961 atataggtga acttgccatc tttgatggtt ttgataacag cactctttac ggcgtcgcca
    10021 tcttcgttga gagtgatcat tccggttgca cccggaaaat cctttgtggc agctattgca
    10081 gcacgaacct tttctttgtc gaggctgccg gcgcgctgaa gagcatcgcg tgcaaccagg
    10141 taaccatcga agccaagcgc ggttactgct gcaggctctt tgttatattt cttgcgatac
    10201 tcgtcgagga atattttgga tgtttccgtg atgggcgtct cggttgcaaa gaaagtagag
    10261 aacacaacat cttctacagc ttctttccca acatcgatga attctggagt ctcccaggtg
    10321 tctccaccca agaatggggt ctttattccg agctccttgc cttgcttgat gataagagcg
    10381 ctttcggtat agttacctgg tgcaaagata acatcgggct tgtgcttttt gatctcggta
    10441 agctgggcag aaaagtcggt gtcgcccgtg ttgtagttca attcagcaag aattgcatcc
    10501 tcatcgccag tcagctgtat gaatgcatcg gcaaagaact tcgccagtcc aacagagtaa
    10561 tcgttcgata cttcgcgaat tattacggct ttcttggcgc ccaaggtctt gtacgcatag
    10621 ttggccataa ctgttccttg gaaggggtcg ataaagcaaa cacggaagta atattcattt
    10681 ccctttgtga ccaatggatt ggtgcaggat agacctatag ccgagacttt tgcattcttg
    10741 acgatggggc cggccgccat ggaaagcgaa gaaccccaag atccaagcac gacattgact
    10801 ttgtccttgt cgacgagcct ttgtgcggca ttggccgcct cgactttatc ggatttgtta
    10861 tcggcaatta cgagctcgat cttatattct ttgtcgccaa ccatgacagt tggatagagc
    10921 tcatttgcca gcttgatacc ttctacttca agtgctccac cacccgcatt tgcgccagtc
    10981 agtggttcga acacaccaaa ctttacggtc ttgggagctc ccggtccgca ggctacgatt
    11041 agcgcagcga ttccaaggga tagcagccca attctcacga ttctcttcat gattacctcc
    11101 ttcctgtatg tccatttcca acactaatag gacattatcc atggtagaac gccgatagcg
    11161 ttctgtcaaa tatattctac accatttatg agatttaata aacgatattg aagcaagaat
    11221 aaccgtcttg aaccctgcat atatcacttc aaaatctcga cggatatagt ctgttcttgt
    11281 cttcgcattg cgcagaaatg aatgaaatga tttaataagc cgaggagaag aatgcatgga
    11341 tagctatgat gttgtgattg tcggtacagg cccagccgga cttggtgccg catttaagct
    11401 tctggaatcg agaccatcct tcaatattct cctcatcgac aagcaacagg tatcttctgg
    11461 cgggcttcgc aacgattgca agatgaattt tacttggcct ataggttttc ctttggaatg
    11521 ttgggacgag gccactggca aacactatct cgaacttaca gaaggcttcc tcaagcccca
    11581 tattatggaa aaacgcaaca tagagattta cagaaagcga gcggagaaga taggagttca
    11641 attgctcgat gttaggcagt cacacctggg tacggacggt ggttttaaac tgatcaagga
    11701 tctcaccgca aggctcagag ggctcggcgc aacttttagc tttaaagaag aaatgcttgg
    11761 agtcgagcct ggatccagag tcatcataac agataagcgc gagctgaaat atggctctct
    11821 ggtagtagca cccggtcgcg gtggcttcgc ttttctccag agacttatgg aagaactcgg
    11881 cattaaattt cgcgataatg tggtcgacat tggcgtccga atagaaacaa cagaggaaca
    11941 ttacccaata gtacgcgatt actacgatcc caaatttttc tttcctaaaa agacacgcac
    12001 tttctgtacc aactcgcggg cagcctatgt ggtgcaggag cgctacggcg acgaaaaaca
    12061 aggctattgg tactcgatca atggccatgc ttggtctagt gagcgaccag caaatggtct
    12121 tgtgaacttt gcgatattaa aaaccataac acttactcag cctctcgcct ctggacagga
    12181 ttatgcgcag atgttgggac atctagcagt attgcttgga ggcggaagac cgataatgca
    12241 gcgcataggc gattttaggc tcggtaagcg gtcgtttgcc aactctttta ccggcgatct
    12301 atataacttc aacccctcac ttccctcctg tacgcccggg gatataagcc tctgcattcc
    12361 agctaaaacg atgcgtgcta tttggaacgc gatgaaattg ctagatacaa tagtgcccgg
    12421 cgttctacac ccttcgacca tcatgtacta tccggagatc aaattgtatg caaaccgccc
    12481 agcttttata gatgagaact tccaggtttt gccaggaatc tactttatag gcgatggcgc
    12541 aggcacaagc cgagggatta cctcggcatg ggcttcgggc atgagagcag caaatggcat
    12601 tattagtaca tcgaatctct gattgctagc aaagaaccag gggagcggta tcctattaga
    12661 gccaatttca agaatcctct cctcgggtta cagagtttat aagttctctt tagaacagaa
    12721 aaggcagcgt cttgtcggga ttcttgccga aacaagtcac ctcatcaata tccttaatga
    12781 atggccctcg gagaagcgca aatagggttc gctctatacc gagccctcct ccgcaaacct
    12841 gtggaaagat cccttgtcct tgagcatctc tcatgtgagc aatgctgagg aatgggccat
    12901 agccatcgag cgctgccata ttctcgatat ttccatttcg atcaaaacgc ggtgattctc
    12961 ttaaaacagc attatccaga agtcgtgaaa cgattgattc atagatccat tcccgcaagc
    13021 caccagagag tatctcgtat gctgcaccaa gactttcatc tctatagaga ggcgcagcgc
    13081 ataaatcgta attgaatacc tgccttggag agatatcttt cttttctggt cttctcccgt
    13141 ctcgagacag ataaggatat acaagatcat agttctctct caaaagcccg agcacccaga
    13201 agaattgtgt tttggctttg tgcccaagcc gctcttccaa gctgccttcc aaactgcagt
    13261 caccctgcat atcaatttca tcgctataga atcttggaaa tggcttcgat ggaacagtga
    13321 gcttgactcc aagtgctccc aaactctccg cagccagcac cagaattttc tttacaccat
    13381 agatgatcag atcctcgaaa aagtcgagcg ccctatccag ctcttttgtt aatacctgag
    13441 tcgtctcttt tggccgattg aaatagtcgt cctcgctaag cctttttgcc cgcttgaatt
    13501 cgatatccat ctgagagaaa tcgattaggt atttgtgtct cgaggttgag gagggcaatt
    13561 cgagacggat attggacgag tctatgtata ttccttcaat atatggattt agacaagcga
    13621 gcattttgga gtagatcatc gaatgtgtgg ttctataagg cgtatcctgg taaagaatta
    13681 caggcacatg ctcgacatca tggcatagag gatctgtgac aggcgagagc gataccctgt
    13741 ctatattcag aattcctcta gaaaccagga attcctgaag tcccagtaca agggctgttc
    13801 gaacctttaa tgcatgggca agttgttcac ctttccattt ttggacataa gcatcggata
    13861 aaaactcatt gaatgagagt cctttatcgg caagagaagc ttgaaagcgt tccaatatgc
    13921 tgccaatcga tgtgccggct gcatctaaag aagcaaatga tttcatggtt tttctccttg
    13981 tggaggaatt cagcggtagc gccagaaccg caggaacgac gttcattgcg gaatactggc
    14041 ctaaaatccg gaatcccatt accaccgggg aaagagtaac taaccgccat cccctgcacc
    14101 tccagtttcg gaaaaatgag cgcaatgtgt agcgctaaaa aaaagccggc cccaaaaacg
    14161 ggaccggctt taatcttcgc gcttcgattc gcctatatat tcggctccgg ccccgctcca
    14221 agaatgcgat tattattatt gttgagggag ttgccattat agccaaatac tacttgcgcc
    14281 atgatcattt tttactccca ttgcataaat atgtcaagag gttccttaag gaaatctact
    14341 tgaccaaggc catgaattca ggccatagtc cgccccatgg aaggcaataa cgcaaaactc
    14401 tctcaagcca tcatggcatg tctttttgca gccctgattt ctgttggagc gtatgttgca
    14461 tttccgattc ctggcacgcc tgtaccgatt gtgcttcaga atatgtttat tatgcttgct
    14521 ggatttattt cgggaccatg gtggggcctt gtttcggtgg ttttctattt gatattggga
    14581 tcggtaggct tgccggtttt ctctggagga actggaggct ttgctaaatt ggccggacct
    14641 acgggcggat accttgtcgg ctacataccc gcagtcatcg tctttggtct tatatcaaaa
    14701 cttggcaaag gcaaatggat tttcaatatc ctggctggaa tttccggaat gataattgta
    14761 tacgctgcag gcatagtctg gctgaaatcg gtattggata ttggctggga aagagcaatc
    14821 gcaaccggag ttatcccttt tctccctggc gatattataa agatcatcct cgcctcggtt
    14881 ttgactccac caattctaaa ggccatgaac catatcgatc aacagacaaa tggctgagct
    14941 atttcgtgcg gtctctctga gtcacatctt tccggataag gggatcggtt tagactcaat
    15001 ctcgctttcg attgaggagg gagaattcct tcttgtcgca ggaagaaatg gtgcagggaa
    15061 gtcgctgctt atgagacatt ttgtgggcct ttccaagcct tcggcaggag aaatactata
    15121 ccggggcatt cccatcgcct tgcaaattcc gctgatccga agagaggtcg gctatgtctt
    15181 tcaggatact gaggctcagg tttttggttt gacggtcggg gaggacctgg cttttggtcc
    15241 cgcaaatctg ggaatcaaag gttcaaagct cgatgccgta atcaaaaaag cattagcaca
    15301 agcccatctc gaaggcatgg agaatcgcag cccgggcagt ctctcgggcg gagagaagcg
    15361 tcgacttgcc atagcaggcg ttcttgccat gcaaccgcgc tgcataattc tcgatgagcc
    15421 tttcgccaac ctcgattatc cttccgtaca ggagattcta gaggtcttcg gtacgcttca
    15481 ctcagatggc aagactctga tagtgctcac ccatgaaatc gaaaaagtat ttagccttgc
    15541 ctcgagactc cttattctcg accgcgggaa aattgtcttc gatggattgc caggagaggc
    15601 aacaatagga ttatttctgc gaagtgggtt agtctgcccc tatcaagagg gttatccatg
    15661 gctgatagaa tagcggctgg aagcacggct aatccatttg ctttcgaacc aggagagagc
    15721 aagctcgtca tgtttcatcc gcttgcgaaa ctggttttct tgattgcagc aacaagcttt
    15781 gcaatgcatg cccctgtggg cttcttgctc gctctttttg taatagggat tttgcccctc
    15841 ttaaaactgc cgagagccgc agcaggccct ttttattcaa tagtcgtcct tatgttgttt
    15901 gccaccatcg ttaggggcat cttcccaggc gacggaagga tatttgacct ggccacattg
    15961 cccggatcag ctgtctatgg actcaggttg ttttcgatat acatttattc caggcttttc
    16021 tatgcgacaa cccgagtaag cgaaataggc gactggatga ctgccggttt aagaagcctg
    16081 agaaaaatcg catgtagaag agaggctaaa gataatccaa cttcgaggct caaccaggaa
    16141 tcgacatact atcgccctgt atctatggac atttctttag atccaggaat gttcgtctct
    16201 ttgatactcc ttttcttacc acgtattttc aatatgtatc gcagagtcgc agaagcaggc
    16261 gaaatgcgcg gcatatctat ttccaaaaag aactttcgtc gtgcctttct tatgctcggg
    16321 caattgatct cgatgggcct cttgcagtcg tggaggatat caatggcaat ggagctgcgc
    16381 ggctactcac catataggag cataagactg tcgagattca agaaacaaga tgggatcttg
    16441 ctgggccttt ctgcaattat gttcctactt tgaaattcta ttttgagaaa tgcctggctt
    16501 cctgccatgg ctcgatcaat gagccttgag cacagttgtc tttctcagtt gctctcaatt
    16561 cccacagctc aatggcctgg agttttccct ctacgatagc cggaccaagt cttcggaaaa
    16621 gcagagtctt tccttccagc tgtggaagtc gtatctcggg agctaattcg ctgtccagat
    16681 cttgcgctgc cggaacaaga cttgaaggag agcttgccct ggaacttccc gcgcaagccg
    16741 cggctaccgt ctcccttatt gtcagtattg cggttcccaa tactttgttt aggttttcaa
    16801 gtcgactcgc tatgttgaca gaatcaccta cggccgtgta ggcaaaacgg tgagtcgagc
    16861 caaggttgcc tactactgcg gagcctgtgg caatccctat tctggttctc ggggatatcc
    16921 cgaagcgctt ttccagctca ggacctattt ctctgagctt ttcttgaatg tggcatgctg
    16981 ccatgcaggc gcgcaaggca tggtcttcga tgtctagcgg agcattccag aatgccacta
    17041 ccgcatcgcc tacatatttg tcgagagttc ctccctgtgc cagtatttct tcgctaatga
    17101 tccccagata ctcattcata aactgggtaa gtcgctctgg atctagtttt tcggaaatag
    17161 ctgtaaaacc agccaaatcc gagaatagcg ttgttatcac tttcgattct ccaccgagcc
    17221 taaggacctt cggttgttcg attagctcgg atatcacttc cggagacaga tactgagcaa
    17281 aagccctacg caaataagct ctctgtctgc cctccgcttg gtaaccaaga actatggcgg
    17341 ctataaaggc aaaaagcccg gctgcaatcg caggagctgc attatagaac acgagatttc
    17401 ggaaaaaagc aaagctcgat ccgattggga ttataatcgc tgcgacaaat cccactattg
    17461 tccagatcct tgttcgcatg gtcggaagaa gagcggccag gacaagagca atgatagtta
    17521 tgcagacttc tacgccgatg ggagaaagct ctataaaaga tgaagaaagc gcattgctaa
    17581 taaatgtggc gtgaatctcc acgccgggtt gtgcgggatt tatcggtgtc gcttgtctat
    17641 ccatgagccc tggagcggtt ataccgatca aaacgatttt gccttcaaag ttcagctcct
    17701 gatttaagcc agcaaaatca gagcccccgg tacctggaga gccagcaagg acttgcgctg
    17761 cactatatcg ctcgaagcga gaagaatcct cgaatttaag aaacaccgtc tgctcgaaag
    17821 cctcaaaagg cgcaagtggc tcgagaggtt caaaggcctc agaaagagag atgcttttct
    17881 cgtcggcaat cgaattcttt ctattaaaag cacttattgc ggccaatccg agagagggga
    17941 tctttccagc atctgtatct aatgcagcct ggtattgcct gcagatacca tctgaatcta
    18001 ctcgggcaac cacatggccg tatagtgcat tggagacggc tagcacaggc tttctgtctg
    18061 taagggttgc caagaccaca ttgcctgcgg acctcattgc ctctgcacag cgagagtcat
    18121 cttcgggacc ataagtggaa ggctccgtaa atagaatatc ataggcttgg acatcggcat
    18181 tgcgtaaaga gtatgctatt agcccatata gttcccgagg ccaaggccaa ccaagaccga
    18241 gctcattctg tacccaatca aggctggatt ggtcgatcat gacaagcgca attggcgctt
    18301 ttttcctgga aggtgaaagg gatgcagaat ctgacgaacc ggaattctta cgtaaagttt
    18361 gggaggaagt attatcctcc ttcttgcctt ccgccgctac cagcaaacgc atctcccgat
    18421 catagagggc cataccagcc ttattggaaa tccctgtcca ctggaacaaa agaattccaa
    18481 agacaccggc tactagcgca tatagaaatc tctttacttt attcaaagca tgattagatc
    18541 ttaaagtgct gtggtcgggc ttgcctgcct ctgtcatata tttcattcta aaatactttg
    18601 agattgccta gaatagaggc cgaatggctt cgaagcgctc ttgcctcaag agctcgagag
    18661 acacagggct gatgattaag ttctcctttg cgagctcata tccagaatca agaaagcctt
    18721 gttcgcaagc tagattacta ttatgccacg actcatcttc gttttgcaga agcgaatcca
    18781 gtctttgtac gcattctttg cggtgttcga gctcggctcg ttgagaatat tcctcagcaa
    18841 ggctgcctat tctcgatagt ctatcttcgg ccaaaggatg ctgcatcgaa tactgctgaa
    18901 aacgctcatc cttagaattc tccagcgtta ctacaaaatc tccgagggct gaaggtctat
    18961 actcggcttc gtaaagcatg cgcatcgctt cggcgtctgc ttcgaattcc atgacttgcg
    19021 aataaccttg cacagatatc agggaaacaa aatcatgcac cgccgccaag aacagctcca
    19081 tagcctcctt attgtaagac tgcgcgctct ctgtggaaag cattacatag tattttgcta
    19141 aatcccccat gcgatagctc gtgagtgcca ataatccgtg tcccaacgct gtatgagata
    19201 tctcatgtgc taaaagagcc gcaagcatgt cctcattgcc tgtcagcttc agaagccccc
    19261 ttgttatcaa tacgaatccg cccggcgcag caaaagcatg cgcttcttcg ctgtcgagga
    19321 ccaatatatg atatccctcc ggcaggcatg gcctggctga ataaaatgcc aacccttgcc
    19381 ctatcttatt tatataggct gttagctcag gctgatcata aacttgatac tgagacagca
    19441 cggcggcggc gactactctc cctataacgt attcttcctc ataggtgaag ggttcagatt
    19501 cgatttgaaa agagcgcacc gcttccaaaa tgagccgcga ttgttcgggc gtaatcatgc
    19561 cctgaagcaa ggcttgatcg agaaaaaact gaagagactc cggcgttatg gtgcaagatg
    19621 cgaaaaacgg taggataaga atcagcgcaa gaataaccga tagcacgcga gattgaagca
    19681 caatcttccg cacctcatgg ttctccttcc aaaatatcta tgccatggat aaatgcaagg
    19741 cattccgcat aagaatagct attcttttcc attgcatcca ctgcttcgaa gtcgaaagca
    19801 cttgattctc tgagcgacaa ttcaagacta gatgaaaagc ccttgccagc aaggacaatc
    19861 tcagtttcct gcagtggagg tggatcggag acatcgtcag ggttcaagga gactttgact
    19921 ggagaaagtg cactcatgtg gatatatccc ggcctattta gtccccgaac aaagccaaat
    19981 atccagtcgt tttcgatgcg tattatggca actggagtct tgtaagcggc aaaagagagc
    20041 gccggcgaaa caaaagaagg ctcggctcta actgcggctt tttcgaccct gatgagcatg
    20101 gtgctctcca gggacttttc ctgcgccgag agaattgtca aacccgcaag agccagaaaa
    20161 atcgcaaaaa accatcgata tttcatagca caccttcttg atgacgtctg ggtgcccata
    20221 acggcgcaac cagcgccagc ctcctatcat acttcatttc ttgatatgcg agcagaaaga
    20281 cgaaaaatca tctgcggctt tcattttgag ccccgaacgc atacattgct actcgcaccc
    20341 acctatacat ataagaatat atcacgcagc aaaacaatct tcagatggct tgactgcata
    20401 gcctatcctt ccatttaata agcgccatag ctataggcct tcgcggatca tccggagaca
    20461 tttcctgcaa ttctctctcc aatctcacat aatccgaaag ggcggtggct cctttttctt
    20521 gtggaagctg tagccatccg tatacatgct tcttccaaga taaatgctga tcagccgtca
    20581 aggtttcagg aaaattgcgc gcaagaagcc tgcccattag ggttggaatt cttggatcct
    20641 gaaatcgaat gcgaagaaac tcgttttttg cagcctgtgg accgctattc attagcactc
    20701 tatggaaacg ctccagctct ctgcgatcct cgtctgaata gaatccgccg gcatataagg
    20761 catgttcagg atcaagctca tcaaccgatt ttctctcaaa tcgataggca gcaatgaagc
    20821 gcgctttaag atcaggattt gcctcgagaa tacgcagatt ctctctgcat tccacaagat
    20881 cgatacccag ccgcgctcga gcttgtcgat ttaacacgct gagaggagca aggtagggac
    20941 agctgttgag gcggaccata ttgagaggtg gacgaatctt ttcatcttcc tgcctctccg
    21001 agaataaaag gtcgcgcaat tcatcttcgg acatatataa aaaaggctta ggatcgaagc
    21061 gcaaatcata ggtcacaaga gcatggcgat catcttccag tagccctaag ggagttatta
    21121 ccgtcgaaca accaccttgt ctcgtatagc gctgtgaagt gtgtacgagc atattctccc
    21181 ggcgagagag atctacaagg cgcgctaagt tctcgcgccg acggtggcgg taataccatt
    21241 cgaagagctt tggataagtc tcgagcacct tgcgggcaag gcctatagtc gcccttacat
    21301 cgtagagcgc gtcatgagca ttctcatggg caacattttg tgctttggca agatcaacaa
    21361 gcttgaacaa tgggttgccc tcgccattta ttggccactc gaaaccttca tatttgagat
    21421 cgtggacggc tcgcatcaga tttattacat cccagcgaga attgttgttc ttgtactccc
    21481 gactgtaggg atccataaga tttctataga aaagattgcg aatgaactca tcgtcgaact
    21541 ggatggagtt gtagcctgtc accgtgctgc ctggaacact catttcgcgc tgtatccttt
    21601 gagagaactc atattcggat aagcctttct cttcggcttc ctgaggagta atgccatgta
    21661 tatagcatgc ctcgggatca ggaagataat cgagaggaag cctacagtac ataacgactg
    21721 gctctgctac ctcttcgagc gctatattcg tccttataaa agcgcactgc gcgatgcgat
    21781 cgtgcctagg attgcgacca aaggtctcga gatcatacca tagaattgat actgccatct
    21841 ttatttcgtt ctatcttcta tagacatttt ctatgctttt tcatcaatag taaacatcgc
    21901 gggattaaaa tcatccgatc ctacccagag atttatcata ataccccctt cgggaatttt
    21961 ttccactttt gcacccttga catgatagcc aatgagttcg agccgggcgt cgcctaacag
    22021 atctcggcct agcattgtcc catagtaaga ggcaagttcc ggtctctgat gcatcttcaa
    22081 accttgctct atttccgcga tctcttcatc gctatggtac cgatcatcta tagcgactat
    22141 atgaaaacca tcgttttcga gatgcagaga tatcaccatc tttttcggat tgttgcgcaa
    22201 aagataatta gcaagctcgc ttatgagttg tacatataga tcgtgccttt tcatcctcat
    22261 tctcctcttg cttggcttaa gaagccctga atcggcctgg atttgctcat tcaaccttgt
    22321 ttgcgacctt actcgaccga taccaatcga taaccgaaac gatcagagtt gcggtaagcc
    22381 ccgccgcaaa gccattgttg tagagagcca tacctccgtg ccaaaagcat gttctatcca
    22441 caataacaag atgcaagaat cccgcaacaa agcctaataa aggcccaaat tctccagaaa
    22501 gaggcgctag tgccgtccca aataggatgg ccaaaagcgg ccccggctca tataaatcct
    22561 tatcgaatac gaaaattgcc accacgatac caagcacaat cggaaaaaca ttcttaatat
    22621 gttttccaaa gaatgaaaaa ccaagaatcg taaaaagccc tcccaagacg ggtccattga
    22681 aaggagcttc gataattagc ataaagagcc agaaggacag tcctaatact atcatgttaa
    22741 gaaaggcacc ctttgtcgat acgaagtcgg aaaaatcact cggaagcctg ccgctcatgc
    22801 ctagaatctt gcggaaatcc aaataggcat gccgaaatag agcgacaatg ttcttgggat
    22861 cttccttcac aatgcaaaaa agagcaatca gcaaaacaat aggtaacaaa gctacaagaa
    22921 ttggactatg agccgtcccc catatttcaa taggtagaat gtctgcgccc gtcgcatggg
    22981 aaagcgaagc cgcaaaaagc cccaaaaaac ctgcggtcaa acccatgttg taaagattgt
    23041 atccctgatg aagccgaagc atggcgatgg caatgggcgg aaggatgagt ccgacgccca
    23101 acccgatagc aaaagaagcc ggtaaggcaa gaaagctctt tactccgagc tcgaaggcaa
    23161 taaaagtaat aaggggaccc atagcagttc cgaatatggc tatgagggcg tagtctctag
    23221 gttttttcct taccaaaagc gcagaaaaag atactccaag cattattgga agacaattga
    23281 atggggtctt accgaaaaag gcaaaaccca tcatcgtaaa aagggctgca agagaagcac
    23341 cagttaacct gagtttatta attgccagaa aaccataacc cagaaagcca accgtactga
    23401 cattgaggag tgtcgaccct acccccgcaa tagctataaa gtcgcttaca agccgagcag
    23461 gtgtgatctg cagctgcaga aaacccagga tggcttgtgt tccgccctct attatcccgg
    23521 caagaaaaat aaagaccagc actatcagca taaaagcatt caaagtcttg ggctgctcca
    23581 tcatgcacct ctctttttcc atcctagaat cattgagccg cataaatcaa gtgttataaa
    23641 gatctgactt gacgaatcct ttgagcgcct tcacaatgaa ataaaaggag ttactcatga
    23701 ataacggaat attccacatt ccaaggccaa tcaacgaacc tgtgtttaat tacgccccgg
    23761 gttcgcttga acgagccaca ttgaaaaagg agctcgatca cctccgcaca aatcagatcg
    23821 aaattcccct gattataggc ggaagagagc tgcgtacggg caagacagct actattcgcc
    23881 gcccagatgc ccatggagaa gtcctgggag tctatcatct ggcaggagaa accgaagtca
    23941 agatggcaat ggaagcggct cttgccgcgc aagaaggatg ggctgccatg ccctgggagg
    24001 agcgcgccgc ggttttccta aaagttgcgg aactcatttc gggtaaatat cgagcgctta
    24061 tggatgctgc aaccatgctc tgccagtcca aaaccgccta ccaagccgaa atcgacgctg
    24121 tctgcgaggc aatcgatttc cttcgattca acccctggtt tatggagcaa atctattcgc
    24181 agcagccaga taatgtaaaa ggagaatgga atagatcggc ctacaggccc ttagaaggat
    24241 ttgtatatgc tgtaacccca ttcaacttca catcgatagc tgcaaatctc cccacttctc
    24301 ccgcaatgat gggcaatacc gtggtttgga agcccgcatc taatgcagtg tactccaact
    24361 acatgctcat gaggctgtat atggaagccg gtttgcctcc gggagtcatc aactttgtgc
    24421 cgggaagcgg cagtctgatt tcgaatgtag tgcttgcaag tcctgagctc gctggctttc
    24481 acttcacggg ctcgacagaa gtatttcgct ctctctggaa acagatttca tcgaatctgg
    24541 aatactatcg gagctatccg cgcattgtag gagaaacggg aggcaaagat tttatcttca
    24601 tcgatccgag cgctgaactt gaacctaccg tagcttccac tctcagagga gcttatgaat
    24661 atcaaggtca gaagtgctcg gcggcatctc ggctctatat ccctgaatcg cgtgcttctg
    24721 aattcataga agcccttgtt gcccagatct ctacaattcc catgggagag atcaccgatt
    24781 tccgcaattt catgggtgcg gtgatcgatg aaacagcctt cgaaaatatt catggctata
    24841 tcgaacgcgc aaaggcacaa ggagcgaagg tgcttgcagg aagcaattgc aataaatctc
    24901 acggttggtt tattgagcct actcttattc tcaccgagga tccgcgctcc gagactatgg
    24961 tcaatgagat attcggaccc gtccttacgg cttatgtcta cgatgatcga gacatggagg
    25021 cggcttacag gcttattgat tctacttcgc cctatgggct cacaggtgcc ataatggcta
    25081 cggatagaga gcgcatcaat cgagctcttc ttgcgtttca aaattcggcg gggaaccttt
    25141 atatcaacga taagccaacg ggagcagtgg ttggcaggca gccttttggg ggagcgagag
    25201 cttctggcac caacgataaa gcaggcagca taatcaatct cttgcgatgg gtgagcgcgc
    25261 tcgccatcaa agagaatttc tctcctccag ccgactggcg ctatccattt atgaaaggag
    25321 acgactaaaa cgcagatgtc gggtgccttg tggtaagagc aagaatattc aatgtccagc
    25381 gccataggga aagtgctcag ttgaagctgc catactggcg gtgagaacct gcgagagctg
    25441 gcggtgagag ctaaaggcag catcattttc gttttgcaat ccctacctaa tcatgctttg
    25501 ccttgcttat ctggctaatc cgaattttgt cagcctcctt gcccactcaa cccagcgcaa
    25561 catccaaaag catcataaga gcgaatccgc acatagtacc tatggtagca aggtggccat
    25621 ttgaatcgcg acttgcttca ggaagcacct cctcggctac gacataaatc atcgcgccag
    25681 cagcaaagga aagcgcataa ggcagaatag gccgcattgc gagcacaagg accgcaccca
    25741 gaattcccgc tatgggctcg acaagacccg atgcttgtcc ataaacgaaa ctttttcgac
    25801 gagaaagccc ttctcttcta agtggaatag agaccgctgc cccttccgga aaattctgca
    25861 acccaatgcc gatggccaac gcaattgcac ccgacaaact agcgcttggt atatcagccg
    25921 caatcgcccc aaaacccacg ccgaccgcaa gaccttcagg gatattatgg agagtaatgg
    25981 caagcacaag aaggacgctg cgttgccagc ttgtatgaag gccttctgcc tcgctcatat
    26041 ccgctccaat gtgcaaatgt ggcaaaagat ggtctacccc tagtagaaac gctcctccaa
    26101 gcaagaatcc aataaccgct ggtagccacg gaataagccc catagattcg gataattcaa
    26161 tggctggaga aagcaaggac cagaaactcg ccgcaatcat cactccggcc gcaaagccga
    26221 gcattgagtc gagaaccttc ttgttgatct ccttgaaaaa gaagaccatc gcagcgccaa
    26281 gcgctgtcat accccaagta aagagggttg caaaaaaggt ctgcataaca tgattttcga
    26341 tattcataga tacctcttag gcctccattc tctctattcg ctattatctt ttatcgctct
    26401 tttaattagt cccggtcggc ttcaatcgct attctatccg aaacaaggta ataaaataca
    26461 ggctgcctat tggacaatcg ccttttttga tcgcctaaca ttgactgtat ttcgagttgc
    26521 ctatatcttt tggttttgga aagagccaga tgaaaattga tttttctcat gaatttaaag
    26581 acaatatgag cggaccattg ggaatgccgc aaaacacaaa gaacatagaa gaaaaggcct
    26641 atgtcgcatt ataagaatct ggtcttcaag ggtggcggag tccgaggcat cgcatatatc
    26701 ggagcgcttc aatacctata tgagcgaggt ataatgcctc atatccggag agttgccggt
    26761 acatcggccg gagcaataac cgcgctcggt gttgcaatga atccaacaga ttttatgggc
    26821 ttcagaacgc tagccgatag cctcgattat cgcaaagttc cttcggaaca ggacgagaat
    26881 accgaaaaac tcacaagtaa gcggctcatc gcaataatcg agcactatcg cgaacttgcc
    26941 atttttaaga acttgcagtg ttccataagg cttttgcagg aaaaaggctg gtattcaagc
    27001 gaatacctct atacatggct tagatctatc atcgccgagt atttcactgt cgccaaggag
    27061 atatatactt tcgccgattt tgccaataca caaatccata gagacggcag acccttccta
    27121 gacttatata ttacggggac cgatgtatcc aatcgcactg ctcggctctt ttcgctagaa
    27181 acaaccccag atatggaagt tgctctggca gtacgtatct cgatgtctat tccgcttttt
    27241 ttcgaagcta tccagtacca gtatccgggc accgaagagc ctcaattcta tgtcgatggc
    27301 ggcgttatgt ggaattaccc aataaatcta ttcgacgatc ataagtattg cagaaagctc
    27361 aatgatgggg caaatgccga gactttaggg ctctttctat tttcttcttc cgaaaagacg
    27421 cactatcctc caataaaatc catgctcgac tatatgagat cactttttga aagtgtttcg
    27481 acggtgcaag agcaactcgc cattcgaaca gagaagaatt attcccggac catttacatc
    27541 gacgattgtg gtatcgaagc cacggatttc gacattcagc caggagatga acgacataga
    27601 atgctcatag actcagggtt cagggcaacc agagagttct tcgaatccag gacagaatgg
    27661 tctcagtttc tcgcattcct tagggagcgc tttggatgga aggagtaagc ctgtctacct
    27721 tcatacaacc gctatacatg agctttttta catatgaaaa acattatgct tgaataatat
    27781 catttatcat gccatattcc atttattcag agcttcgttc tttaggagta tacattcatg
    27841 gatcttgttc ccgaaaaaaa gccaaatcct gctatcaagc aggtcatcgg tgtaattagc
    27901 ggcaaaggag gagtcggcaa atctatggtc gcatctctcc tggctcaatc ctttgccgca
    27961 cgcggcttaa aagtaggcat gttggatgca gatattacag ggccgagcat tccccggctg
    28021 ttaggcatag attgttttcg cggtgaatcc gatggtgaaa acctctaccc tgtgcaaaat
    28081 gaggaaggaa taaaggtgct ctccataaac cttttcaatg aaaatgagga tgatccagtt
    28141 atatggcgcg gccccttgct cgccaaggtc atcgatcagt tctggtcaga tacaatatgg
    28201 ggagatctgg attatctaat catcgatttc ccgccaggta cttcggatgt agcactcacc
    28261 gcattccaga ccattccctt ctcgggatta gtggtggttg ctacgcccca agattatgtc
    28321 accatgatag tgcgcaaatc ggtcaatatg gcctccatgc tcaagacgcc tgtgctcggt
    28381 gtggttgaaa acatgcgtac aatggtttgt cctcattgtg gtgcagagat tgctctattc
    28441 gatgatggca gccaaaatgg agctaagcgg atggaattgc ctcttcttgc aagtttgccc
    28501 tggagaaaag aacttgctca agcacgatcc cttcgatggt ccgctctcag ccaagagatc
    28561 aaaagggatg ctgatcgtct cgccaatgag gtggaatttg ctctgacggc attttcgccg
    28621 aaatgagaaa tcacatacga caagttttct tttcttgttc cactttgtct ttgtagaagc
    28681 ccctttgccg gtcatgccct gttcctcatt cctagatcgc tcatgacatt agcgcaacaa
    28741 tcatgctaaa ataaacaata taaagaacaa tctatagtag aggagtttgc tatgaaagga
    28801 aaacgcttat tgatgattcc aggccccata gaatttaccc ttgaggtgct agcggaaatg
    28861 gcaaagccga cactgagcca taccgatcag caattcatcg aagaattcgg tcaagcactc
    28921 gagaaaatgc ggaaagtctg gctcgccccc aacggtcagc cctttgcaat tgctggaagt
    28981 ggtacacttg ccatggagat tgcagcagca aatataacag aaccaggaga taatgtgctt
    29041 attgtgcaca ctggttattt cagcgaacgt atggccgaca ttttcaggct gcatggcgct
    29101 aatgtggata tagttcaaag tgcggtcggc gatattccgg aaatagatga ggtgcgcagg
    29161 agactcaaat ctaagaatta caagatactc agcattaccc atgtggatac aagcacggga
    29221 gttcgcaccg atgtaaaagc actctccgca gccgcacagg agacgaacac gcttgtagta
    29281 gtggatggag tatgcgcggt agccggagaa gagcttcgca tggaagaatg gggagtggat
    29341 attgccttta ccgcttcaca gaaggcaatc ggcgttcctc caggccttgc gctccttgtg
    29401 accagcaaaa aagccatgga gtcttttagg tctagaaaaa gcccggtagg tgcctattat
    29461 tgcgactggc aatactggct gcccgtcatg gaagcatacg aagggcgaaa atcggcctac
    29521 tttggaacgc ccgctgtaaa ccttgtacga gcactaaata tcagcctagg gcagattcta
    29581 gacgaaggca tggaggccag gtttgaaagg catgcgcgaa acgccgcggc tttccgagca
    29641 gggctatcca ctattggcct tagctttgtc cccgtgcgta aagagataat ggctaatact
    29701 ctcagtgccg tatattaccc cgaaggcacc gacgcaagta ttttgccctt aattgcaaag
    29761 aatggcgctg tgctcgccgg gggcttgcat ccagcaatca agacaagata tttccgaatc
    29821 ggccacatgg gcatgagcga tgcttcagag atacttgcag tgcttggctc tatagagcaa
    29881 ggtcttgcga cagcgggcta ttccttcgaa ccgggcgcct cgctcaaggc tgcccagtcg
    29941 gcattgattc agccctgatg acatcacttc agatttgaag cggtgttcgc caatccaagc
    30001 tcgaactggc gcaacaaaat agtgggaatt cttctcaatc cgctttatgg aatattccac
    30061 atacgatatt atggatcgta tgtggaattt tttcgtacct aaagagtcgc aatggtattg
    30121 ctggaagctc gatggagcct ctctctggat gcgcaaaaat ggagaagaat ggcggtttgc
    30181 cgctagctcg atccctttca aacagattca aaatgattcg gctggccctc ttttcgtcga
    30241 tgctccaagc gagctatcga tcgcctttgc ggtcgctcct ggcaaaaaag cagccctgcg
    30301 cccacatcca agtactgcac cttacctcgt atcggcgcga aacgatgtca aaatttaccc
    30361 tgggaccgaa gctcagttcg atatcgcact tccgcccata tttcggatag aactagaaaa
    30421 cggattgtcc ctcttcgaaa gtcttccatt tgtaacccct tatacctggt ttggcgataa
    30481 ggtaagcgga gatctctgcc tttcccttcc catcgagcta gatcctcagt gcaagaatga
    30541 acaaggctat tttgagagaa tagactctaa taatcctatc tctctcgatc ccataatgca
    30601 ggaatcgctt caaagagccg ctcagtactt atcgtgccgc tcgctgattc attgcaaaat
    30661 cgttgtcaaa aatagatcca aagaacctct aaatgtaaaa cgccttgcca tttttacaag
    30721 cctcatgaat gtctaccaga aacaagataa tctcgtgtcc gataccgtca taatcacagc
    30781 tgccgcggat gattcgctgc agacaaatat cgatgatagt gcttataaga atcttccaaa
    30841 gattcatgca gcccctaaga ccggactgaa tgagctgctg gttatgaaag gcgtaagttt
    30901 cttgcgcagt attgccggcc tttaatgagg agaacgaaat ggatgacaca attttccctg
    30961 attccataga tcgctttata caggatgccc ttacgcccga gttctggagc aaggctttta
    31021 cgatcctgat cacggcgatt ctaattcttg tctttttcag aatactacgg ataattctag
    31081 gccgtgggat gaaaaagact atacccgaac aaaaagcaca gctcatctta aaagctctcc
    31141 gctacacagg ctgggcaatc gctgttgtat ctgtcttgca gagtgtaggc gtcaatcttt
    31201 cggctttgct cggagcagcc ggaattgcgg gaatcgcaat tggatttgct gcacagacct
    31261 cagtgtcgaa ccttatcagc ggtatatttc ttgtttttga aaaacccttt caaataagcg
    31321 acaccataca gatcgatgag atatccggaa tcgtaatgtc tatcgacctc ttatcggtca
    31381 agcttcagac cttcgataac cgctttgtcc ggattccaaa cgaaagcata ataaagacaa
    31441 acgtgatcaa tctcacccgc tttccaatcc gcaggctcga tatatatgta aatgtctcct
    31501 ataccaccga tctaagaaaa gccacggatg ttctccgaga tattgcatac aaaaacatct
    31561 atgtgctcga taatccatct cccctcattc ttatagacaa attcgatatt ccgggcgtag
    31621 gtattcttct cggtgtatgg ttcgaaaagt caaatctcgt agaaataaag aatacaattc
    31681 taatggatat tcaagagcga tttgccgaag aaggcatcga aataccgtat ccgaagcgcg
    31741 atgtctatat caaaggcata gcggaaagaa acctgatcga aatagaccgg aaagaaaaca
    31801 cttaagctag gttagcaata tatccctgct agcttgtgat attttcggac ttttagctga
    31861 tattcttgat cttcttcagg gctccgagca gccttttctg tgtcgaagta ttataaggcg
    31921 gatagaattt ttcaaccgcg cttatggggc cctgatataa taccgttctc tcgtgcgaga
    31981 aagtcttaaa gccatagaaa ccatggtagc ttcctgttcc gctcatcccc acgccgccaa
    32041 aaggagcatc aaggttttcg atctgcacaa taaggtcatt tatgccgaca ctgcccgaag
    32101 tcgtttccgc caggagcctc tctgcgcttt ttttatcttt tcctagaatg tagagagcaa
    32161 gtggttttgg cctagacctt ataaactcaa ttgcttcatc gagtgaatcg taagcaagaa
    32221 ctggcagtat tggaccaaag atttcctccg ccataatggc catatcgatt tgcacccctg
    32281 tgagcagggt tggcgaaaca tatcgattct ccacatcgaa gcgaccaccg aattcaagct
    32341 tggctccttt ggaaatcgcc tctgagacaa ttctctcgag acgagcacag gcagccttat
    32401 ccacaatgcg tggataatct ggcgaagcct gcctctcttc ttcggacttt ccatagaatg
    32461 attcaatagc caggcgcatc tccaccgaaa attctccaac caactccata gggcagaaaa
    32521 gataatcggg cgcaatgcat gtctggccgg cattgagaaa tttgccccaa gcgattcgct
    32581 tactggcttg ctttaggttc gcgcctcgca acagaatggc gggcgattta ccaccaagct
    32641 ccaacgtgag ccccgcatgc atgcgcgcag cagcctgacc taccttgact cctactgcag
    32701 ggcttcctgt aaaaaatatg tgatcgaacg tgagttctag cagaaaatcg ccaagcgtgc
    32761 tgcctgaacc ctcaacaatc gctacctctt gggaatcgaa tactccggca ataaggtctg
    32821 cgataaacgc agcagtatgc ggtgtcttat ttgagggttt tgcgatcacg cagttacctg
    32881 cagcaaccgc cgagacaagt ggtgcaatta gaagctgaaa cggatagttc catggcgcca
    32941 tgatgaggac gcgccctttt ggctcatatc gcacacgcgt agaagaaaaa ggcaaaatga
    33001 gagtcccctt tgcgcgcctt cctttcatcc ataccttgag atggcgtact gcaacatcga
    33061 tttcttctat gcaaggaaaa acctcagtta accaggcttc aaatgcaggc ttaccaaaat
    33121 cggagttcaa ggccgcatat aattcgtcgc ttcgtgatag tatcgccgag cggagctttt
    33181 ttaatttgac aatcctttct tctggcccag taagggctag tttccagcgc cgctccgcct
    33241 gtgattcaaa gagtttatca agctgttctt tatcctgaat gaccattttc cttgctctcc
    33301 cttgcatttc ttataccata aaaatgcttg aactgtagat aaatgtaaga cttacctgac
    33361 ctgtcattca tactagcttc aaatactaag caataacatc ttgcaagata tcgccgcgaa
    33421 tttggctttt attctaaaag cccttcgata catttttatt attggttctt ttaatcgact
    33481 attcaaggat tagggaatta aagaagaagc aaaagctatt ctcttggcgc tgagcatggg
    33541 catgctgatt ttcgggtgta cgattgtggt taccccatca tgtgaccaat ggagcctata
    33601 ttataaatga gaaggatccc aagaagcgta tgcagtatcg acaacctcaa gtccctcgac
    33661 accgccttct taaccatcat ataccttagc accacctact attattcccg gttctggctt
    33721 ctaatagata tcaaaacact actgctacga accttgtgta taagaagttg aagtaagaag
    33781 gaggtaatag cccgaataag ggaagagcaa ggagctaact atgcagactt tccctttctg
    33841 ccctaatccc aaatgtctcc atcataaggc agctccaatt caatctattg aagcgccccg
    33901 ggtttactat ctcaaaagag agtttagaaa gtgccttgcg gatcatcgca gagaaacgct
    33961 gtgctttgca aggaatgtgt ccaatatgct gcatggactt gtcttgtaca tataagaata
    34021 aagcggatta tgtacctggc tatacttacg cacaagaagg ctttcatatg ccattctcaa
    34081 tccttataaa caaggttcgt agcagtattg aaatatcgat aaaatggttg taggatagag
    34141 tgggtccggt ttcgagaccg atttctaata tcttatatct tagaggaata ccacaatgcc
    34201 taataacaag aacatggtaa tgatcgacgg aaatgccgcc gcggctcatg tcgcacacgc
    34261 atgttcagat gtcatcgcta tctatccgat tacaccttct tcgcccatgg gcgagcttgc
    34321 ggatgagttc tcttcaatgg gcagaaataa tatctggggt actgttccgc aggtagtcga
    34381 aatgcaatcg gaagccgggg cagccggtgc ggtacatggt gcacttacaa ccggtgcgct
    34441 atccacaacc tttacggctt ctcagggtct tttgctgatg attccgaata tgttcaagat
    34501 cgcaggtgaa gcaataccta ctgtattcca tattgccgcc cgcgctattg cagcgcatgc
    34561 gctctcgatt tttggagatc atcaagatgt tatggctacc cgtcaaaccg gctgggctct
    34621 tcttgcctcc aacaatgtcc aggaggtaat ggatcttgcc ctcatcgctc acgctgctac
    34681 actcaaagca cgcgttccat tccttcattt cttcgatggc ttccgcactt cccatgaggt
    34741 ttccaaaatc gaggaactct cttatgacat aatacgagct atgattcccg aagagaaagt
    34801 gcgagagcat cgcgcccgtg gactcagccc cgagcaccct gtgattcgcg gcacaagcca
    34861 aaatcccgat gtctactttc aaggacgaga gacaataaac aagctatacg aggcaactcc
    34921 tgctattgtc gaagaaacga tgaagcagtt tgccgaaatg accggacgag cctacaagct
    34981 cttcgactac tcaggcgctc cagacgcaga gcgcgtcata atactcatgg ggtcgggcgc
    35041 cgacacagcc gaagagacgg ctgaatacct tgcgaaacaa ggtgaaaagg taggtgtcat
    35101 caaagtccat ctctttaggc cattctctgt cgagcatttc ataaaggcaa taccggcaac
    35161 ggccaagtca atagctgttc tcgatcgcac aaaagaatca ggtgctattg gcgagcctct
    35221 ttacgaagat gtgcgaaccg cgataggcga ggcaatgggc aatcataccg cacctttcaa
    35281 gaattggcct atagtggtgg gcgggcgata tggacttgga tcctatgaat tcacacctgc
    35341 aatggtcaag gcggtattcg acaaccttaa gcttgagcat ccgaagaatc acttcaccat
    35401 cggtatcgac gatgatgtta gctttacgag cctgccctat gacgagaaat tccacttacc
    35461 aggcgatggc attattcagt gtctgttcta tgggcttgga tctgatggaa ccgtaggtgc
    35521 aaacaagaat tccatcaaga taatcggcga tgaaaccgaa aactgcgctc agggctattt
    35581 cgtctacgat tccagaaagg ccggcaccta cacgataagc caccttcgct ttggtccgaa
    35641 gcagatcaaa aagccatatc tgatcacaaa agccgatttt ctggcatgcc ataagttcag
    35701 ctttatagag aggatagaca ttctctccaa tatcaaagaa ggcgggacat tccttctaaa
    35761 tagcccttat tctgccgatg aagtctggga caatttgccc atagaagtac aaaaggaaat
    35821 aatcgacaag aagctaagct tctatgtaat cgacgctatg gccattgcag acaaggctgg
    35881 aatgggcacg cggatcaaca ctattatgca gaccgcattc ttcaagatct ctggcgtact
    35941 gcccgaggaa caggctgtcg aactcatcaa gaagtacaca gagaagactt atctgcgaaa
    36001 aggcgccgat gtcgtccaaa agaatctcga tgcaatagat ctggcactga atgccacgca
    36061 taaggtcaag attggccaag cagacagcat taagcacatg ctcccgccgg tgccacagac
    36121 ggctcccagg tttgtgcgcg agactctggg cgagattatt gcgcagcgcg gtgagaaaat
    36181 gcctgtttcg aagctgccac tcgatggaac ctttcctacc ggcacaacgc agtacgaaaa
    36241 gcgcaatata gcggagaaaa ttcctgtatg ggagccatcg ctttgcactc aatgtggcaa
    36301 ttgcagcctg gtctgccctc atggagtaat ccgcatgaaa gcttatgatc ctgcaatact
    36361 cgagaatgcg ccttccacct ttaagagcac cgacgcaaaa ggcaaggaag tggctggtct
    36421 caaagtcacc gtacaagttg cacccgagga ttgcactggt tgtggagcgt gtatcaatat
    36481 ctgcccggct atcgatagag ccaatccagg cagaaaagcc atcaacttcg gaccgcagat
    36541 cgagcttcgt gacaccgaaa aacaaaactg ggacttcttc ctctctattc ctgagaccga
    36601 cagcaaattc ctgaacctct cgattccaaa aggcatcgca atgaagcagc ctctattcga
    36661 attctccgga gcatgcgcag gttgtggcga aaccccatat atcaagctcg tgagtcagct
    36721 gtttggcgac cgagcaatca tagccaatgc gactggctgt tcctctatct atggcggtaa
    36781 cttacccact acgccctata cgaagagagc ggacggtaga gggccggctt ggtccaacag
    36841 cctattcgag gatgcagccg agtttggata cggcatgcgc ctaacttcgg acaagcagaa
    36901 tgaatatgcg cgagagcttc ttgcttcagc caaaggtaag ggtattcccg aagaactcgt
    36961 agacaggttg ctttccaata ctcaggatag cgatgaacag atcgaagctc agcgcgccga
    37021 tgtggctgca ctcagagaat cccttgccgg caaatccgaa agctgggcta aggaactcga
    37081 caatgtagcc gaagctctgg ttcgccgctc tgtctggatt ttcggcggag atggatgggc
    37141 ttacgatata ggtttcggcg gtctcgacca tgtaattgct tcgggcaaaa acatcaacct
    37201 ccttgtgctg gatacagaag tctactccaa caccggaggc caggcctcta aagctactcc
    37261 aattggagca attgctcgct tcgcaacggc aggcaaggag acttccaaga aagaccttgg
    37321 catgatcgca gtaagctacg gatatgtcta tgttgcgcaa attgccttgg gagccaatat
    37381 ggtgcaggca ataaaggcca ttcgtgaggc tgaaagctat aatgggcctt ctattgtcat
    37441 agcctattcg cattgtatca accacggtat agatatgatg aaaggtatgg atcaacagaa
    37501 aatggctgtc caaagcggta tgtggccgct atatcgctac gacccgaggc tcaaggcaca
    37561 aggaaagaac ccattccagc tcgattccag agagcccgac acttctaagc tcgagcgtta
    37621 catgtacgcg gaagttcgct ttaagagcct tgtccaagcc aacccagagc gcgcaaaggt
    37681 tcttctggag aaacagcgcg agcttatcgg acggcgctat aatgagtatc gctatttagc
    37741 ggacaggcct ttctgatctc tggaatggtc tctatctcat aggctccgct tctaaatctg
    37801 aaagccactc caattagggg tggctttttt actttccaat attgaccttt gcggccattt
    37861 tcttctaaag tgacaatgct cgatgaatac aataaaatcg tataaatgga ccattgcaag
    37921 cgcaatggta gtgctcctcc tgcttcttat gccatttaga atatttcaac aaacccctca
    37981 aagccttaat gactttgata aattcataca tgcaatcctc tttggagcca tgacagcagt
    38041 attttgtgct gaacataggg ctctaaagaa aatcaaccca aaattccttt tgagcctcgt
    38101 aattataagc gcttttgcat ttctgaccga gatcggccag cttcttacaa taaccaggca
    38161 tttcgatttg aaggattttg gagcggatgc aattgggatt gctgcagccc tgttagtcat
    38221 gcgaatcgcg acaatgcttt cgtgagtttt tcggcaatta gatatcatat ttgatatcaa
    38281 atagaaaata ggaaatactc tactcaggga gaatcgcgat gaagaatctt gaaaaacagg
    38341 gtttccccgc tccaaagaat gagtcgactc ctgcctcgga aaaatctgca aaaatagaat
    38401 cacttactct tttgcagcta caaaaatcta gctttttatt tttcttgatt acgagcatgt
    38461 tcgtcgtttt ttgttgtata ttgatgatat tgatgcccga aattgctttt ctaacctact
    38521 attcgccaga aatgctcata cttattgggc tattctcttt tgggtttttg attgtccacc
    38581 ttaatagaaa attagcctgg ctcgggcctg tccttattat tgctcttttg ccattgactc
    38641 tcacaaggta tttgactttt atgtacagta tttgcgcctt tatcatcgcg cttatcgaac
    38701 tcaatcgcct gggctttttt aagcatgcag gcctattcaa agcttgcatc cttatctcct
    38761 actatgcctg gaattttgcg ctggtcgcca tcatgcataa agaattcttc ctcgacataa
    38821 ttttgtcgat tgccttccta tctctgtttt ttatttattt gatcccaata attcaagata
    38881 gatcgcagat tgggaaagtt gatctagctt atagaattta cccaagagat ttgaatctta
    38941 cctgccgaga gatggacttc ttaagagatt gcctcaatgg agccactttt aaagagatag
    39001 cttataagca ccatgtaagc gaatcgacag ttcgtaatac ttttgctcac atctatcata
    39061 aattcaatgt agtagacaga atagacctta tgtcaaagct tgctgctttt gatatcatcg
    39121 acgaataact caaaagaggg atttatggat ttagattcat tggctctgag aaatatcgca
    39181 ggagagaacg cagatactct tcttgcagat gcggatagga ccgcaaaggc ttttaggtat
    39241 ttctatgttg tggtatcacg ccttcgtgca tccgatggct gtccctggga cctcgaacaa
    39301 acgccatcga gtatccgcgg caacattatc gaagaggcat acgagcttgc ggaggccata
    39361 accgaagatg acagccttca tataaaagaa gaaacgggag atctatatct tctcgccact
    39421 atggtaggct acatgtgtca gcaagaagga cgtttctcag tggccgatag tttgtgcgat
    39481 gaagcccgca aactgatccg tcgacatcct cacatatttg gtgagtccga ggtccttagt
    39541 ccggatcaag ttgtgcggca atggaacgaa atcaaagagc aagtcgaagg ccgtagaaaa
    39601 aaagagtcaa tcctcgacga agtccatcgt agtctgccgc cattagaaaa agcctataaa
    39661 cttcagaaaa aagcggcaaa agtcggcttc gattggcagc acgagcgaga tgtttggaac
    39721 aagctggaag aggaattagg agagctaatg caagccaggg cctggaaatc tgaagaatgt
    39781 ccatctgaca tagctaccga cagcggatcg agcaaggatg cactcgaaga ggaatttgga
    39841 gatcttcttt ttaccgtaat aaatgcagcc cggctttacg gcatcgatcc atcgatcgcc
    39901 ctacagcgca ccaatggtaa attctcgcga cgattccgtc atgtcgagga gagcatgaag
    39961 cagcagcatc tcgatatgaa tcaagaaaat atggatatga tggatgcatt ctggaacgaa
    40021 gcaaaagggg cagaacattc ataacaaaac aagcgcctat ccgagatagt atcgagtagc
    40081 acatcctctt gctatagtat atattcgaca atatggccat tctgctttct gctcagaagc
    40141 ttgcaaaggc acgcaagaca ttcaatattt acaacttttt aaattctttc tctttcgtgt
    40201 ttgtatctgg atcgattatc acgctatttg ccctaaaatt gggtgctaag aatagtatca
    40261 tcggcctttt gaatgccctg gcctatgtaa ctttttttat gctgccgctg ggcaagcgtt
    40321 tggtcaaaag gtcttcgata ataaaggtat tcggctgggg ctgggttata aggtatatcg
    40381 ccctagtacc aattctgttt gcccctctat tcgcctcgaa ggggcatatg ggtcttgcga
    40441 ttcttatact cattctgggt acagcaggat tccacatatc cagaggaatt gccctgatcg
    40501 gaaataatcc ggtgttggga ctattatcca ccggaggtgg cgacaaacct cgaagtgatc
    40561 aagggcgata tctagtcaac atatctgtcg tcaactcagt agcggcaatt ctggccaaca
    40621 ttgtgctggc atttttgctt ggccaagaag cctcgctatg gacctacaca attgcaatag
    40681 cccttggaat tattaccggg cttatcagtt gtgcaatact attcaagacg ccggaacctg
    40741 aagccttttc cggcaaagat gagcaatctc tctgggatgt cacaaaagag gctcttgtcc
    40801 agccggattt tcgcgcgttt atattgactt tcatgttgct atccttcctt tccggcatgg
    40861 ggcgcgcatt tctgccggtc tatgcaaaag aagtcttcgc tcagggcgat gatgcgataa
    40921 tggtatattc gatcgtagca agccttggct cgataacgat ggggctcata acccgcctgg
    40981 tcgtagatcg cctcggtccg aagccacttt ttataatcta tagcgcaatc ggatttgttt
    41041 cctttcttcc aatcgccctt attcctggtt ccgcgacctt aatgggcgca ccatcggtta
    41101 tagcattgtt tctggccata gttcattttc tttcctcttt tggatttgct ggtgaagaga
    41161 atgctgcaca ggtctattat tttggcctgg tgccaaaaga aaaaatgctc gatttggcgg
    41221 taatctacaa tctagcctat gggattggag gatctctggg ttcattcctt gggggcggaa
    41281 ttctcgaggt ctttgcgaat atggaattgt ctgatgctgg agccttcaga ctctactatg
    41341 ccgcgatcag tgtagggctt gttattgtga tctatagcat gcgaaatcta ccacacatgg
    41401 gtgcaaagtc ggtaactcag tcgatctcta ttttgctttc tcctcgagaa ctacgagctt
    41461 tcgatctatt gacgagactc gaccagagcg caagtgccca tgaggaagtg cgcatcatcc
    41521 aaaaactcag caagtcggaa tcgaatctta cgcaaaaaga acttgtggag tatctctatt
    41581 cgccacgttt cgaggttaga tccgaagctc tcctcgccct cgaatcaata ccggagctcg
    41641 atcatgagac aattgtcgcc ttgatacgag aagtaaaagc taatgtctat accacagcct
    41701 atgttgcggc tcggatactg ggcaaacagc gatccagaat agcgattcca gcgcttatcg
    41761 agtcgctgga tggaccagac tatatgttgc agagcatggc tatgatagcc cttgccagac
    41821 tccaagcctt tgaagcagtt ggcaaaatcg aacagcttat gcgatctatg gagaatccac
    41881 gagttcgaat atccggagcg tatgcgctcg agcttttcgg aagaattgac tctattccta
    41941 cactgatatc ctgtcttaaa gccgaggata gacctgccag tgtatccgac gaacttgtac
    42001 tgagcatatc aggcattctg ggcatcgata aggagttcta ccttctatat tcggaattca
    42061 tagatgacaa ttcctatggc attgcacaac tcatagcata ttcaaaggat ctgaatctcg
    42121 gagatgatag attgaatgta tggatttctg cagtggatgc tctatttgcc cctgattccg
    42181 atactgctcc tcttgcgcgg ctacttctcg caagccctag aataaccaat tctatcctca
    42241 tgctaagcga tggacttctg gaccccgggc ttacgtatcc tggctttaat tttctatgtg
    42301 cctatattgc tctccatata gaagatctga aagactatta aaagtacacc acnnnnnnnn
    42361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaaaagta caccacgatc tatgctgccc
    42481 cttcttccta gattggcagc gacatcaaaa gggatgtgag aagcctgacg aggaaacctc
    42541 ccagagtgcg cattacaaga accacaataa aacccgtgat gagaccttgt agatattgta
    42601 ccgttcgacc gcgatagata atcctgttta ttctaaaaag aagagggttg atgattccat
    42661 ccagaatctg ccaaagagga tgaagcacgg ctatcctcgc aaggaaacct atgatcctta
    42721 taagcgatat cacaaggaag aaacccaata aaaaagatat tgctgaccac gccgcatcca
    42781 acaaaaggct tagaataaaa ccaagtgtta tacgcacggc ataagaaagt gtcataaaaa
    42841 gattattgag caccgataat actgcaagcg ccactatagg actaaaatcg atagccggcg
    42901 tgcggaaaag agaaaaaccg cgaaaaaggt tgagatacgg atccgttaga ccagcaataa
    42961 gcctgcccca ttttgtatcg gtaagcgaag gtatccaaga gaaaaatatt cgaactatgc
    43021 atagaatcat gtatagcgat acgagactgc tcactatctt agcaagggtt gaaaatacca
    43081 tgaattatcc tccgcacatc ggttctttat ttagaaacca atacctacta tactaaaaat
    43141 cgatgcatat aggcaatgtt catatcggta cctgtggatt ttcctacgag gaatggaaaa
    43201 ccgtatttta cccggcagat ctgccttcga atgaaatgct gcgatactat tcgctggtct
    43261 tttccttcgt agagctggat aacacatggt atcgcatgcc taatcccgct cagctcaaac
    43321 ggctggcgct cctaaccccc agcgatttcc gcatttcgtt aaaagtacat cgcagcctta
    43381 cccatgagat agacgaaagt tggcaaacaa aagccaatga attctccctt gctgtctctg
    43441 cgctatcttc gacggggaga ctgggatgtg tccttgtgca acttccatat cgattctctt
    43501 atacgataga aaataggaaa tatcttgcta acttgctcgc agcgctctcg gactttcctc
    43561 tggtcgtcga attccggaat gccacctggt atcaagacag agtttttgaa acacttcgaa
    43621 agctaaaggt tggactcgta acagtcgata gacccgatct gcctggtctg cctcctgaat
    43681 cgattatcat aacatcggac atttgttatt accgtctgca tggtcgaaat acagatcttt
    43741 ggtggtctgg caatgcgaag agtcgctacg aatactgcta ttctgagcag gaattgaagg
    43801 atagagcccg acttatacgt ataatggcca aacagagcaa gaccgcattt gttgcattta
    43861 acaatcattc caatggttat gcgcccaaga atgcggcgta tctgcaatcc atcctcgctg
    43921 ggcagcaatt gtgagaatat tcctagctca tggttgaccg gtgtctactt ccatgccttc
    43981 ttcggacagg ttttgcctta tctcctttgc ggcaaaggtt tcgatctcct tccagagtcg
    44041 ctcccagtca tctaaaagca aatcttctgc ggtaaagcaa ccatcgatag acggaacaaa
    44101 aagcaccatt tttctaataa tggatctatt ggtttcgagg agctgcctca aagcctcgcg
    44161 ctggcgatta tcgaggatat cacggcttat aacttcgatt agcccctcgg agtagagcgt
    44221 cacggatagc cagccacttt tttccacaaa atcgattgta tgaagagctc cacttgcaat
    44281 tcccggatgt gaggctagcc agctctcatg aaatgaaggc acggcaatgg tccgaccatc
    44341 ggcaaccacc caccatgcac ctgtcgaaat ctcaaatggt tttctcatcg ctttgctctg
    44401 tattcctttt atcgagtatc cttccttctc gctccagagt ctgccttaga gagatgaaat
    44461 gagcaggttc agctgcaaaa agtggtagat ttggtgactc aagagatggc aaaggcagca
    44521 cgagttcgta tgcccataac atgagacgtc ttattccatt ttttcgcaga gctttattga
    44581 gcgaaaaatc gccatactta tcgtcacaga taattgggag accaatacct gccaaatggc
    44641 gcctaatctg gtgcatacgg ccagtgctta actctagatc caaaagcgat agtgttagaa
    44701 aattggcttc cgcggctgca attgaggaat ttgcctcata ccgatcaacc cctattctat
    44761 tattgtggac atgcccccca tcttgcacat cagcccgccg attggagtgc tcttgtaagg
    44821 gaaataacca ggtctcacgc agggtccaaa gagtacatgc ggcctgaatt ccccttttta
    44881 catgcagagc atcctctata gttccttttt cctgattagg cagcccggca acccaggctt
    44941 gataacgttt ggttatctct ctgcttgcga tacgattcga ccatttgtta gcagccctgg
    45001 catcttttgc gagcatcatg cagcctgccg tatccttgtc cagcctatgg acgggaaaca
    45061 gtctgtatcc gaactggcgc tcaagagctg taatgacatc atcgcgcaac ccttctcccg
    45121 gctgagctgc aaggcccgct ggtttgtcta taacgatgaa ctcatcgttc tctaatagta
    45181 tgcgaatcat cggtgcttca tgatatcaga acaatggact ttccgcaaag ccaagtctgg
    45241 aatagagtgc acaacagatt tatcgtttgt cggatcagta attcaaagag gagtcgtgct
    45301 gcaccccaca ggcaggctca aaaatgatgc gttgcttgca tcgagcgctc taaagaggaa
    45361 attgcgctgt ccagatcgta agaaatgaga acattgaaga gaatatcgat cgccgcgagt
    45421 tgactaattc tggacaactc ggctgcactt cgatagatcc tctctaccgt cggtatttca
    45481 aggataagat cggaataatc ccgtagcctg ttttggccgg tcatcgtcat cgaaataaga
    45541 aaacagcctc tggcttttga ttgagccgct acttctatca tttcgttggt ctctccggaa
    45601 tatgatatta tgaaagccag atcagattgc cgaagagaac aggaagccgt aatctgtgca
    45661 tgagtatcga atagatatga actgggtatg ccaattcgcg taagcttttg atggaagtcc
    45721 tccgcaacca atcccgaagc tccaataccg aatatagcgg tcatactagc tcctttgatt
    45781 cgctccgccg cttcttccac cgccctcgga tcgagtgtct gagcaagaga attcatggta
    45841 cgtcggactg cttcggtcat accgcgaata gcgcgagcgc caggcgtctg cgattccagc
    45901 tccagatcgg gtaggaattt ttcgccccaa ccttggtaca catcctttgc aagccatagt
    45961 ttgaaatcgc tgtatttgcc tacgcctata tgacgacaaa agcgcactac agccgcggga
    46021 cttgtcccgg cttgcctagc aagctcgctt atgttgagat gcaaggcttt ttgcggagct
    46081 tcctgaacga aagcggcgat ctttcgctcc gcttccgcaa gattaggaat tgccgcgtct
    46141 attgtttcga gcgctccttg attcatttct ttcccctttg ttttcttgct tgctatctcc
    46201 cttgctctct gaataaatgc gtattgcctc agaaagccgc ccttcgccgt gctcgataag
    46261 ctcggctgcc tgttctgcgc caatgccgag tatggtcatt agtatagcta tctttggctt
    46321 gcctccagaa gccagaaacg cggcttcggc ttcatccttg gaacaaccgg ttgcttgcct
    46381 gataagccgc ttcgatcgcc ctatgagctt acgatttacg ggcatcaagt caaccatgag
    46441 gttgccgtag actttgccaa tgcggatcat actagaagtc gtgatcatat tgagcacaag
    46501 tttctgagca gtccctgcct tcatccgggt cgaaccggta atgacttcag gtcctacctc
    46561 taaaagtatc ttatgccgtg caatatcgaa agtccttgac tgcctattgc aagaaattgc
    46621 cgcaactacc gcacccaaat tcctcgcgta ctccatcgct ccaagtacat aaggtgcggc
    46681 tcctgaagca gtaataccaa caagtacatc ctgttcggaa aaatttatat ctctgagcgc
    46741 tttttcgcca ttttctggat gatcttccgc tccttcgatt gaatttctga gagcagcctc
    46801 tcctccggct ataagaccga caaccatatc tggcggaact ccaaatgtcg gcggacattc
    46861 tgaggcatcc agcacaccta gccttccgga ggtaccagcg cctatataca ctagtcttcc
    46921 accactattg aaggccttta cgacatcttc gacaagcgct gcaatgctgg gcaaggcctt
    46981 ttccacggca aagggcacac ctttatcttc ttcgttgatg atcctcaaga cctctatcac
    47041 cggtagagca tcgatatgtt cagaacgcac attgcgcgat tcggtagtcg cgcgttccag
    47101 gtattcatcg accgaatcta tatccatgga caagcctctt ctgttaaaat tatttttcac
    47161 atttttgaga gaattgcaag ctcatttgtc gaaaaacaga aaaaatattt cattttatga
    47221 ttgacaaccg caataaattc atcaatacta ataaaaaacc atggatccat agagaaaatg
    47281 gacctggaca tattcatcaa ggaggctgtc gatggcgaaa aaactatcaa tcgtattgct
    47341 gcttatagca atactttctc ctatgtatgc tcaacagaaa acagtaagca tctggggttg
    47401 gagagctcag gatgtcgatg tatggaaagg ggtcgagacc gcacttaagg caaaaggtga
    47461 gcaaatatcc atcagatatg aggttttccc acctaccgaa tatgattcta agctattggt
    47521 ttcattgcag ggcggagttg gtccggatat catgtatacc cgcagactcc ccggtgcccg
    47581 gacccaagcc ttgctagaca acggctacct tactgccata aatggactgg tcgatctatc
    47641 caacttcgat gatgtatcgt tgagttttat aagcacggat aataaagtat ggggcgttcc
    47701 ctttgcaaac cagataattg gaatattcta caatagggca atctttgata aatacaaact
    47761 caaggagcca caaacctggg atgagatggt ccagatagcc gagaccctaa agaagaatgg
    47821 tgtaacgcca ttctttgtta ccggcaaaga agcgtggatc cttgccatgc aaaacgccat
    47881 ggttggcgtg agctatccgg gagacgaatg gataggaaaa ctaaccgtag gcaaggcaaa
    47941 atttacagat ccagaatatg taaatatgct caaagacctc aatgccctta aaaaatacta
    48001 ccaaaaggat tttatggcca acaccagcgc cgaacaggat gtagcttttg ccatggaaca
    48061 ggcggcaatg gtcttctatg gtgtctgggg caatacaaat tggctcaaga tgaatccaga
    48121 cttgaagttc gattatttcc ctattccgcc aaaggataag aaagtaccag caaaagcgta
    48181 tacatacatg gatggcgctt atggcctaaa caatacttca aaaaacaaag aagctgcatt
    48241 gaaagttctc aattatactg gtaccaaggc ttatggtgag ctattctcct cgacaaccgg
    48301 cgaaatcacg gctataaacg gcgtcaccat gccagcctcg aagccgatac tggtaaaatg
    48361 ctatgaaaaa atgaccacca tagccgcaaa aaaccgctac tgggtgggct caccctttga
    48421 tgcaggaacg ccgagtgtct acaacattct ggaaaaagac atgcagtcga tgtatcttga
    48481 tcaaatgacg ccagaggctc tggcaaaaag attgcaggat ggagtatcga catggtaccc
    48541 tgctttcaag aaataatctt tctaagtata tattgagtgc agctgtatga aaaactgcgc
    48601 cgcggcttta ttctgagaaa aagccgcggc gcttgcgtat agcgctgaaa cgaggttgct
    48661 gtgcgaaaat attcttttct atggatgctt ctacctgcct ttattctata ttcgcttttt
    48721 atagcctatc ctttcttctc gagcctcggc ctcagtttct atgagtggcc aggcattggc
    48781 ccaaagaaat tcataggcct tgccaactac aaaaacatac tcactgggtt catgtcatcg
    48841 gaattctaca atgcgctatg gcacaatgta gtgtttttta tttggtcact cattttatct
    48901 gtagtacccg gactattctt tgcatttctg ctttccgcga atatcaaagg aacaggctgg
    48961 ctcaaggtgg tctttttctt ccccaataca cttgcaatcg tagtggtcgg attcctttgg
    49021 ggactattgc tcaatcctca atggggccta ataaatcagc ttttcaggtt agttggcctg
    49081 gacttcttgg caaaaccatg gcttggagac acaaagactg cactgccaac gattatcttt
    49141 gttacagcat ggcgaggctt aggattctat atactcatat acctcgccgc gattttaggc
    49201 gtagacagag aaatgataga ggctgcacgc atagatggag cttccgaact gcaaattgcg
    49261 ggccgtatca ttctgcctca tctctatccg ataatcgcaa ctacatccat gcttaagttt
    49321 atctggacat tcaatatctt cgacattgta tatgcgatgg aaggcacaca gggaggtcct
    49381 gcggggtcta cagacgttct cggaactctt ttctaccgaa ttgcttttgg aggacttggc
    49441 gcaagccaag taggaatggg acttggggca accgttgtga cgctgatatt cctcatagtg
    49501 ctaccagtct cgatattcta cgtttttatt gttgaaaaac gcgtagaaaa ggggcaatga
    49561 tgttcaagtt taatggattc cagaaggcgc tagcatggat ttttcttctt ctgctcgcgt
    49621 ttgcaaatct atatcccatc ggtatcatga tgctatccag cttcaaatct acaaaagaga
    49681 tatttcttga gccatttagt cttccaagcg tctggagatg gcagaactat gtctccgcct
    49741 ggcaacgtgc tgattttgca acttatttca agaattccgt tctagtgacg cttatgtcaa
    49801 tcgtctcaat cattatcgta tcttcgatgg ctgcctatgt aatcgcgcgc ttcgatttta
    49861 agtttaagcg aggcatatat ctttacatgt tagctggtct tgcactgcct acacgccttg
    49921 cgattatccc aattttcctc atcatgcgga atcttcatct cttggacaag ctttcgggtc
    49981 tagtggttgt atacactgcc agcggaattg ctttttcggt ttttttgctt gtgaattttt
    50041 ttaaaaacct tccgcacgat attgaagatt cggctaagat ggatggtgcg ggacctttcc
    50101 gcatatactg gcagatcgat ctgccgcttt tgaagccagc tctcgtaact gtggcaatgt
    50161 tcaactttat agatgtatgg aatgacttct ttttccccct aatccttctg agttccaagg
    50221 ccaaaaaaac cgtccctctg gggctacagg cattttttgg cgaatatact gtggaatggg
    50281 atgtgctctt tgcagccctc aatatatcgg ttttgccaat tctgatcgta ttcctgattc
    50341 tttccaagca attcatagca ggtctgacgg aaggcgcctt gaagtaagga aaaggccaac
    50401 tgccagggct ttgaagcgga aaaacaagca gcaaaacccc aagcaaatga gtagcttagc
    50461 ccttctgggc gccatatata agatattttt ccctaatcct tagaaattga ctttcacctc
    50521 tgtgcgcaaa ttccagacag tcttcgagag aagccccact tgttatcata tatcgtagag
    50581 tagccccacc cgccagctta tcgaaaaagc taaggctttt atcatcccaa tcctctcgcc
    50641 atctaaattt gtccggatac attcgatgaa ttgtatggac aagagttatg cctgtatcga
    50701 gcgatcgcag agcttgtcta tcggtaagcg aaaccaagac accttcacaa agctctccct
    50761 tgtatttgga ggccgaaggc gagaaaaaca gagagatgaa caccacacca ggcaattcaa
    50821 aagcagcaag ctgttctctc agagcctctc catcgataaa tggcgctcca atgatctcga
    50881 aaggccgtga ggttccccgt ccttcggaaa gccaggtgcc ttctacaaga caggtaccgg
    50941 gatataattc ggcacagctg agagtcggaa gattaggtga tggcagaggc cagggtaggt
    51001 ttgtcccttc ccatgcgata cgcgaattcc agcccgacat cttgactatt tccagctcac
    51061 agttgggata atactcgccc tttaggtata gagcaaattc tccgattgtc attccatagc
    51121 gcggtggaag cccatagcca cccacaaagc tttccgcttc cttagaaata ggagaacctt
    51181 cgatcttcga tcctccgata ggattgggtc tatcgagcac aataagcggt gtccccactt
    51241 tttcacatac ttccataata taagcaagag tatatagata tgtataatac cgacagccaa
    51301 catcttgcat atcgaacaca agggcatcta aagattccaa catccatggc tctggcctga
    51361 ggcgctttcc atatagagaa tatgcaggaa ttcccctgaa gacctcatca tccagcccca
    51421 cggcatcctg cgcagcgcct ctgaatccat gctccggtcc aaagagacat gctggaataa
    51481 ttccatgttc tatgaaagct ttccatgaag gtatgcctct ggatgttatt gccgcagaat
    51541 tggtagctaa tccatagcgt ttattatatc gacgagtatc atccagaaag ttttcaagac
    51601 ctaacacaac tgaagcccgt tgctgttttg acttactcat gatatatgct aacatattta
    51661 tttatgacct acaacaaaaa taaaggctcc tcacataggc ataatccaac cgaggagtct
    51721 ctcgagaacc tcataaaaga tgcactatcg aaaagcctct gtacaggcat tgcggctgca
    51781 gtttccatgg atggcaagct cttctggtcg cattgttccg gatttacagc aagagaagaa
    51841 cctgctgctg cccaatctat tgaaaaccat gaccaggctt ctaaagtggt aattgctccc
    51901 atcgatgccg agactcgctt tgatgttgct tcgatgacta agcctttgag tacaagtctc
    51961 cttatcctga aagcactgca agcaggcgca ttggatctta aagatcccct tggcaaatac
    52021 ttgccggaac tgaattacaa aacaggacaa atccctatac ttgctcttct tacacacacc
    52081 agcggactac ctgcaattcc tgccctgcat agcttattcc ctgattcaga gaggctcgat
    52141 cgaaataggg ctttcgaaaa gctattttct atcgaacccg aatttgccat tggcaggcat
    52201 gtagtctatt catgtactgg ctatatattc ttgggtgtgc tactggagcg catctccgga
    52261 atgcttcttg gcgagctttt tcaaaaagaa attgggactc cgctagggct tggacaagcc
    52321 tcttttgcag agagcatagg cacatcaggt gagcccaaac ccctcggcaa cgctgttgcg
    52381 acagaatatt gcccatggcg ccatagaaga atacaaggcc aggttcacga tgagagttcc
    52441 tattgcctgg ggggtcattc ggggaatgcc gggcttttcc tgtctctaaa tgatgcaatg
    52501 gctatgggaa atttcatcat gcaggaagga actttcgatg gaaaccagat tttgaagccc
    52561 gagataatcc aactattatg gcgagaatat acccctggtt tagaagagcg tcggagtctt
    52621 ggctttaagc ttcacgattc tacgaccatt gatggcccac tttggccccc tcaatctttt
    52681 ggacatacag gctttgtcgg tacaagcatt ttcttcgagc cacagcgaag gattttctcg
    52741 attctcttaa ccaatagagt ctattttggg agagaacaaa ccacagattc tatcttcgcg
    52801 ttcaggagag agtttcattc gagagtatgg cgcgcttttg ccgaataacc aagatatcca
    52861 gaaattcgca ataggcattt tgtgaaatat ttttttaata ttaaagctcc ttctttattg
    52921 acatctgaaa aacaacctat ataatgaaaa tatattttag ggctttgccc atgcttgcaa
    52981 gaagccccaa aagaattaca ttggcagaac tgctgccttt tcttcaaggc ctaagggtgc
    53041 atgtactctc gttcttgcag agagatgagt tttttctgcg tgctaattca aacccgaatt
    53101 cagtttcgcc acaaaaactt gaagtggcgg aattcaatcc ggagataaaa attctatagg
    53161 aggtatctat gaaaagggca attgcgttat ttgtgctggc tatcgccatt attgcatcag
    53221 tgccggcgca agctccggtc gtgcctggtc ttggcaacaa gaaaataacc atcaatctat
    53281 ggacccacga agatcccaac aggtccatta tagaaaagaa atatattgcg gagtttatgg
    53341 ctgccaatcc gaatataacc gtcaactata atacctatcc atcgaatagg atattcgagg
    53401 ccttaaccac ggcttttgct ggaaaacaag gcccggaaat attcaatgtt tcgataggca
    53461 atgctgtttc atttatcgac aatggaagag tctctcctgt tgactacaag gctctggggc
    53521 ttaaaggccc ggtcgatctt gaagctatgt atcttcctgg tatgctcaat gctgttgaaa
    53581 gggatggtga ttactatgga cttcctctcg aaattacaaa tatgtgtctc tacgtaaacc
    53641 gcaaaatgct caaggaagtc ggcatcgatc cggacaagga atggccaaag acatgggaag
    53701 acctcattag tttatcggaa aaaattgtca aacgcgaagg tcagattata acccgacgtg
    53761 gctttgattt ccgctacgat gcatatacca atctgttcat ccccatggtc gaacagctgg
    53821 gaggagaata tgtctcgaag gatggaaaaa aggccatcat cggcgacgaa gcctggctca
    53881 aagccctcca gtggatgaaa gaactggggc cagccggaaa gaatcttggt tcaccgacct
    53941 atacagcagc tcgaaccgca ttcgataaaa atgatggtac cgttgcaatg tccttctcgg
    54001 gtctctatca agaagctcgc atgaaagcat cgaacccgaa tttttataat tcgaaagatt
    54061 ggatggtggt tcctttccca caatggaaag attcaaaaaa gaaagtaaca gccaactatt
    54121 ccgctcatta ctacatggtg aatgcagagt cggacaaact agtccagcaa gcctcgtgga
    54181 tgttggtagg atggatgctt agccacggag aagagtacct agaaaaagtg gctataattc
    54241 agccaacaaa gaaactaatc gagtcgccaa tctataagag catgccatat tcagatgttt
    54301 ttgcgaaaga cttcgaaaac gcgaaaattg tctatgttgg caaggcaagt gcgcgacttt
    54361 cgaccttgct gagcgagacc attcagaatg tgatggttgg aggtgtcgat ccaaaggtgg
    54421 ccttagaaaa attgcgcaaa agcgctcagg aagctctcga ttctgccgat taaatttgtt
    54481 cttcgtccag agaccaaaat gaaagggcat tgatgatgga agatcgccga aatccactag
    54541 agaccaaacg caacagatgg ggctggattt tcgtagcgcc gggaatatta ttcttttccg
    54601 ccttctcgct atatccaatt ctatatgcaa tctggactag ctttcataac aagaaattgc
    54661 tttcactcaa accaccaaaa ttcatcg
//
LOCUS       DAOOWY010000002        48874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_779_length_48874_cov_9.311395, whole genome shotgun sequence.
ACCESSION   DAOOWY010000002 DAOOWY010000000
VERSION     DAOOWY010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 48874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48874
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_779_length_48874_cov_9.311395"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK105_00230"
     CDS             <1..369
                     /locus_tag="PK105_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60159.1"
                     /translation="LFTIDERIEMLNAMNRFGEQMKVCKWDGLVTDFARKHGCSVLVR
                     GLRNAAELPYETTMAYMNQRLDPSIKTVFFLYDAKHVDISSTLVRDLVTHNRLPDDIV
                     PQEVEKVLRQILQRRGCPLS"
     gene            489..674
                     /gene="rpmF"
                     /locus_tag="PK105_00235"
     CDS             489..674
                     /gene="rpmF"
                     /locus_tag="PK105_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOU60160.1"
                     /translation="MAVPKFRTSKARTRRRRSINMKLSAPTVVHCSNCGNPVMLHRVC
                     PKCGFYRGRQVITPESK"
     gene            686..922
                     /gene="acpP"
                     /locus_tag="PK105_00240"
     CDS             686..922
                     /gene="acpP"
                     /locus_tag="PK105_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOU60161.1"
                     /translation="MDELFEKIKKIIADKLEVDESKITMDASFRQDLGADSLDTYELV
                     YGIEEELGIQIPDEKANEFETVKDAYEFIKTQVK"
     gene            936..1745
                     /gene="rnc"
                     /locus_tag="PK105_00245"
     CDS             936..1745
                     /gene="rnc"
                     /locus_tag="PK105_00245"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOU60162.1"
                     /translation="MRRKDHLPSNNRLEISSSRRSALLRFQRATDISFHDITLLDIAL
                     THSSSVNEKSPYIQDNERLEFLGDSVLNLCISHILYESLPDNDEGELAHMKNVLVSET
                     SLASIAISLGIGEFLILGKGEEMSGGRRKPAILADATEALLGAYYLDSGIQSTKEIVS
                     RIFSSKIDELRKTPSKDFKSIIQEYAQKRGLEIPSYRVARIEGPDHARHFFVSCVIDG
                     ETFGPMEGMSKKEAEQRVAQHIFEVLHKRDAETARLLDIVTKGSTEIDEKI"
     gene            complement(1758..3131)
                     /locus_tag="PK105_00250"
     CDS             complement(1758..3131)
                     /locus_tag="PK105_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU60163.1"
                     /translation="MIIPSIDPEVRRIADRIIQSGKKAFLVGGAVRDAFLRKPVMDYD
                     IATDATPEECMSLFPFAIPTGIKHGTITIVPRSRRYKVEITTLRKEAAYSDGRHPDSV
                     TFVDDITQDLSRRDFTINAMAIDLMCNAFIDPFDGEKDLKDKIIRAVGNPMERFQEDG
                     LRTVRAIRFSAQLEFAIEPNTLVAIAKCSDTLSKISTERIRDEFSKILLTAKPSVGLR
                     LLSDTNLIKEIIPELEICKGISQPDHGEQDVYNHLLSTVDAVIPDNLSEDKVLILRLS
                     ALFHDIGKPACYRAENGKISFYRHEIESEKIARQILLRLKYPNRVIDKVCHLVRYHMF
                     NYEPGWTDAAIRRFVARVGFSSIPDLFTLRLADTMATTGKPISWPALAEFRERIDRIL
                     EEKQALSLKDLAINGEDLAKIGIPRAKQMGEVLSELLDNVLEDPSLNTKEKLLEIAKG
                     RYFEGFK"
     gene            3187..3828
                     /locus_tag="PK105_00255"
     CDS             3187..3828
                     /locus_tag="PK105_00255"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOU60164.1"
                     /translation="MKALIVTGGDNPPSSIIWCLSKKADIVIAADAGLEVCKIANIEP
                     HLAVGDFDSVDPAILSQVSPEKILRFPEEKDRSDTELAIEAAHRYGAKYIILAGAGAG
                     RLDHLLAVRALFERNDPIKEWHTAHESVFLVARGLRLQASMSINSIVSVFPVSGGASG
                     MLSKGLKWPLDGLKWKRGEFGLSNKTISEEIEIYSGENSILVILPLGSKVSVS"
     gene            complement(3823..4311)
                     /locus_tag="PK105_00260"
     CDS             complement(3823..4311)
                     /locus_tag="PK105_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOU60165.1"
                     /translation="MEYLFNFNKIGYLQGRRPKGCILCLVRDDSKEVEKLVVDETGHF
                     IVSINLYPYNPGHLIVFPKRHIVDIREYTDDEERELDRLVKTCLKALDKFGSPSAYNI
                     GYNMGLSAGASIEHLHMHIIPRYPREIGIVELIAGSRVLVQDPKDTLLQLREIFGNLA
                     IL"
     gene            4822..6291
                     /gene="gltX"
                     /locus_tag="PK105_00265"
     CDS             4822..6291
                     /gene="gltX"
                     /locus_tag="PK105_00265"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOU60166.1"
                     /translation="MSVRVRYAPSPTGNQHIGGVRTALINYLFAKSQGGTFILRLEDT
                     DRTRYSQEYVQNLYDTFRWLGFYWDEGPDVGGPKGPYVQSERIDLYRRYAEELVLMGK
                     AYYCFCDSERLERLRLEQISQKKDEIGYDRHCRFITEEERAKRLAEATPYVIRLKIPL
                     HGVTVFHDALLGQIEWKNEDISPDPVLLKSDGFPTYHLANVIDDHLMGITNIMRAQEW
                     IPSAPMHKIMYDAFGWEMPELCHLPMVLGKDGHKLSKRHGATAVKEFRKAGYLPEALI
                     NYIALLGCSYEDGRDLYSLDELVRLFKIDRLNKAPAVFDYQKLEWFNGQYIRMKSDKE
                     LAELIRPYLIDAGLRTEEDPQKDRMELAAIPLIKERLKLLGDSPAIMSYLYKRLEMPP
                     AEAFIPKRASMDEAIMYLEECRSIIQDSNLDDIPAVEERFKEFATVIGKKLGDILMPL
                     RVAITYSRVSPPLFESMRILGKEECIVRIEAAIAYLRGA"
     gene            6360..8087
                     /locus_tag="PK105_00270"
     CDS             6360..8087
                     /locus_tag="PK105_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOU60167.1"
                     /translation="MSESIEKPEYLDFIREAVAEDLRIGRCKEVRTRFPPEPNGWLHI
                     GHAKALFVDFGVAQDFGGKCNLRMDDTNPEKEDMEYVEAIKRDIKWLGFDWEDRLFFA
                     SDYYERLFELACKLINKGLAYVDDLDEEQIKEYRGTNVPDKNNITFTPPGRNSPWRDR
                     SPKENLDLFERMRAGEFPDGSKTLRAKIDMAHPNLLMRDPVMYRIRRIPHYRTGTRWC
                     IYPMYDFAHPLSDAIEGITHSLCSLEYEIHRPLYEWFIQNCEVFPSRQIEFARLNLSH
                     TVLSKRWLLQLVRGKKVKGWDDPRMPTIAGMRRRGYPAAGIRDFLGRIGIAKTDSLVD
                     IQLLEHCIREELNAHANRYMAVMNPVKLVIENWPKGKIEWLEAINNPEDPSAGIRKIP
                     FSGTLYIDREDFKETPPPKYYRLYPGNQVRLRYGYVVTCTGFEKDPISGEIERIRCVY
                     DPNTRGGDAPDNRKVKGTIQWVCAMHAVPIEARLYDHLFDAERPMEVPEGKTFFDNLS
                     DHSLIIESRAVAEPAIAEIGKGETVQFERIGYFCKDLESTSDLAIFNRTVTLKDTWAK
                     IEKKLQRNP"
     gene            8145..9521
                     /locus_tag="PK105_00275"
     CDS             8145..9521
                     /locus_tag="PK105_00275"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOU60168.1"
                     /translation="MSETVVTMEKIVSLAKRRGFVFQSSEIYGGLSGAWDYGPQGIEL
                     KNRIARFWWKEMTQMHDNIVGLDAAILMHPRVWEASGHVENFTDPLVDCKKCKSRFRA
                     DQIPPENLAAKKCPDCGGELTDTRKFNLMFKTTLGPVEDESGLVYLRPETAQGIYVNF
                     KNVQQSNRMKIPFGIAQIGKAFRNEIVTKNFIFRTCEFEQMEMQFFVKPGTDEDWFEY
                     WRSERWSYYQKMGVNMDKLRWHKHGPDELAHYAKDAYDIEYEFPMGFKELEGVHNRTN
                     YDLGRHQQYSGKDLEYIDQDSGNERYIPYIIETSAGLTRQLLMLLCDSYEEQKISDKG
                     NEDDWRTVLHFHPVLAPTTVAVLPLMKKDGLAELAQDIRIELKDDFVTDYDQSGAIGR
                     RYRRQDEIGTPFCVTIDYQTKNDNTVTVRDRDSMLQERISRANLTSYIQHKIKNYHAP
                     NRSEVACS"
     gene            complement(9671..9970)
                     /locus_tag="PK105_00280"
     CDS             complement(9671..9970)
                     /locus_tag="PK105_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU60169.1"
                     /translation="MPSTGKPLTKVEIIDSLAKSTGFTKKDVNLLLDEFAKLAYSEAK
                     KNKKFTIPGIAIMKVVHRKAKVGRNPATGEQITIPAREVIKITVAKPCKDAVLGE"
     gene            10216..11445
                     /gene="tyrS"
                     /locus_tag="PK105_00285"
     CDS             10216..11445
                     /gene="tyrS"
                     /locus_tag="PK105_00285"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOU60170.1"
                     /translation="MNRALEILKERGFVQQCSDLEGLSKLMDERPITFYIGTDPTGPS
                     LHIGHMVPFFAMRHLIGAGHRGIALLGGGTARIGDPSGKAEMRKLISYEEIDANTERF
                     KAQLDNFIHFDGDHALTANNKDWLSNLNYIEFLRDIGRHFSVNRMLSFESYRMRLETG
                     LSFIEFNYQLLQSYDYLQLHKLHDCRLQIGGDDQWGNIVAGIELIRRVEGAECFGLTF
                     PLVTTSDGRKMGKTERGALFLDPDITSPYEFFQYFRNVPDGDVERFLLMFTFLPIEEC
                     RYLGSLKDKALNEAKERLAWEVTSLIHGQEEADKALSAAGAAFKGEGDASQLPTIEVE
                     ISRLDSGIGVLDLFVEAGLAPSKSEARRLVKQSGASVNGRKIENEMQTLTSSDLIDGA
                     LMLKAGKKRVARIVPKS"
     gene            11453..12178
                     /locus_tag="PK105_00290"
     CDS             11453..12178
                     /locus_tag="PK105_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU60171.1"
                     /translation="MQHLFLALADIHGNINALEQILKANSNIEGIFVAGDLTNFGKEK
                     EAQRVLSAFSSIAPKAKLYFLAGNCDTAEARKCFDMQPGYIEQRCCALLPEKEIWIIG
                     CGGGLYHTGLTPFEIREEGLESGIKQALKCAIRGYRDISSMDGSEPTPGLIVLTHTPP
                     KDTFSDMRHSRHIGSAAFQTFLYEQEPLLWICGHIHEGKSIHIENKTLVVNPGPAAHG
                     LYSLILLKKSPKGWKAAAELRSI"
     gene            complement(12194..13177)
                     /gene="hflC"
                     /locus_tag="PK105_00295"
     CDS             complement(12194..13177)
                     /gene="hflC"
                     /locus_tag="PK105_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOU60172.1"
                     /translation="MKKASIIITVVVALAILFIVIFGPLFVLNEGEQAVVVRFGKIVN
                     SHSDAGLKWRSPIIDTVIKYPKRLMSWDGEPQRIPTKENQFIYVDTTARWRIADPIKF
                     YESVNDLNAAYSKLSDVIDSAVRTVISSNYLREAVRSSDEILSENQVESFQTGDVENS
                     ATLQQLAITPTQYEKIEKGRLELTEEMRALVSDIVPSFGIEVVDILPRQIKYSDELSE
                     SVYQRMIKERNQIAQAFRAYGEGKKAEWLGKLENEKRTILSSAYAKGESIKGKADAEA
                     SKIYADSFGRDPSFFEFWKAIESYRKTMPAFNKTLSTGMDYFKYLYSATGR"
     gene            complement(13174..14163)
                     /gene="hflK"
                     /locus_tag="PK105_00300"
     CDS             complement(13174..14163)
                     /gene="hflK"
                     /locus_tag="PK105_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOU60173.1"
                     /translation="MADIINPKKKLNVKLKWPIVIIIIVALAVLVFLLTSFVVVDQTE
                     KAVITTFGKYTKTLGAGLHYKLPFGIQKAHLVKTQVVQTEAFGFRTVKPGIITQYSNE
                     KFPDESTMLTGDLNIVDVEWIIQYRITDPKAWLFNVMDKEKTIRDTSQMVLNMLVGDR
                     TILGVIGPERQSIQETAVTLMNEFFSIYGLGITVTQVQLQNIVPPEGVQAAFEDVNKA
                     IQDMNRLINEGREAYNAEIPKVQGQADQVLEVAQGYASERVNVAKGDVSRFNSVYEEY
                     RKAPEVTRKRLYYEMMEEIFKDQEHVDLIDKSLQNLLPIKNLAQTPSSQEASR"
     gene            complement(14141..15205)
                     /locus_tag="PK105_00305"
     CDS             complement(14141..15205)
                     /locus_tag="PK105_00305"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU60174.1"
                     /translation="MGKLLIITLSRDDDGRRADRIVRKVLKDTALSTIYRLFRQGDIR
                     ISNIPIAPNDRLKEGDVIEIKFSERSLEKGQNQTIIKSTLPKKEYSQVAFSEIPRILW
                     QNAQLIAFDKPRGILVHDGSSSLNAYVQRILYDQLEPSASFSPGPLHRLDRNTSGIIF
                     FSKTRKGAELFSVAMKNRRIRKFYAAIVQGKIDEPISLKDFILRDKKRKLSLLDDRKG
                     KEASLYLSPLMHNKSYSFLLIELITGLTHQIRAQLASHGYPLVGDSKYGGSKTAKISS
                     YFLHSCCLGFPEPIFEDMPLTIFDPLPGDFNLAVSDLFQIDHEEVDALISHRIEQSLE
                     ICDNNLFPEKGAKHGRYYQS"
     gene            15353..17269
                     /locus_tag="PK105_00310"
     CDS             15353..17269
                     /locus_tag="PK105_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1925 domain-containing protein"
                     /protein_id="HOU60175.1"
                     /translation="MQNFKVVIGMYNNLPYNASGALYEQTYQGAWRPFLSGLYKFLSI
                     KSAIYFSGAIFPWLEENHPEYMYLLVEMVRRGQVELLGGGLYNPLSPLISAQDMTGQV
                     ESLSAFIRKTTGKRPSGAWLYEYSWSSSLPSILQNSKIQYTFLPAEYFPNVEGGMEFG
                     LPVTSEDHRKVITIFPTFESKDSENSFMPYENTLTDLQESYPECKTFIIMADGNEISM
                     SWEKSGLESPDVLFERTFAWFQKNCLEYDTLTPIQLHKTTKSSRIVYFSQCYSRRFKE
                     YCKKTINRLPIQNQEYIQISKQSVLDHPLVHVLYQKLNYVSTMIALFRGDKSRKKASI
                     DDIWKAQCGDLFWIGPCGGILIPEARIAAFSSLIEAEKTIRQSRFRPYLSFDDLNFDG
                     LKEAIFQSSVYNCYLQSGSASVSEFDSIKTGTNYACGWNDDQFSTGCFKDYIKTEGNF
                     ERDLIAVERWNMVENPREESSVLFRREFSARFEDRLLMLLCRKTYRFRNDFFSIDYEL
                     SNKNTEACLFRFCTNSEIIAAPIFEDHRFELLHHRESKALDLRSQAFFETVDGIGLSN
                     VRKAERVLIRSDLPFSLLAKPNFLKKPSVSPQVMDVPSDSLMFEGFSIGIGWDLSIPA
                     EGTAFFSLSVHLEH"
     gene            17348..18883
                     /gene="murC"
                     /locus_tag="PK105_00315"
     CDS             17348..18883
                     /gene="murC"
                     /locus_tag="PK105_00315"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOU60176.1"
                     /translation="MEMIQFPDKLEGLHIHLVGAKGTGMTALAEILQKRGAILTGSDV
                     ADVFYTDRILHSLGIELFENFDKKNLPISTDIVIHSAAYSREGNPELKAATHRGIPIF
                     SYPEVLGALSLHSSSAGIAGVHGKTTTAALAGILIKALGLPATVLAGSAISNFDDRCT
                     LVQGESFFIAETCEYRKHFLNFKPTWIVLTSVESDHQDCFPTYESIRDAFVEYVLSLP
                     PNGVLIFCADQKGAVEVADIVLQRRRDIKYVEYGFLAHGKYGIRDFQTKAGASSFILG
                     ESSFPIRIHLPGRHLVQDSVAAIALVEQIFETVKSRPFEESEWISVGEALAAFRGSKR
                     RSEIIGEYKGILIMDDYGHHPTAIKTTIEGIKKFWPHRRLIVDFMSHTYTRTIALQDD
                     FISSLDGADSVLLHKIYASARERPVLGFNGRTLYQKLCEHRPELFPMDLERDTRQKEI
                     AYYKNKASQDQTGFVAYSDEPLKALDALSNLLQPGDVFLTLGAGDNWKLGKALAEKIE
                     KNG"
     gene            18930..19796
                     /locus_tag="PK105_00320"
     CDS             18930..19796
                     /locus_tag="PK105_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOU60177.1"
                     /translation="MKSMTGFAHSPILEKEFQGTLTLKSFNNRFLDISIFLPSYASRI
                     EPLIRDFLGSRILRGKVECTFKLRKIKFSLEDIELQFQAATLLSEQLRKLAEACGIEK
                     DLSLDLITKFPGIVDMNTEIEEDYLWPVLMPILEETWLKFEESRIREGTATEENIRGQ
                     LQRLKSKLIEIELQADSLERMVYDQLVARFKELLSESYDEGRVLQEVAVQLVRLTINE
                     EIGRLRAHIDAFELMAKEPACGKKLDFLCQEMNREINTIGSKSMLVSVSHAVVEMKDA
                     LENIREQLRNVE"
     gene            19786..20349
                     /locus_tag="PK105_00325"
     CDS             19786..20349
                     /locus_tag="PK105_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU60178.1"
                     /translation="MWSRIGMGIIAISGKSGCGNSTVLRLVAEKLGYEPVNYTFRSMA
                     ADMGISFQELHKKAQENLEFDRRLDEHQVLLARKGNTVIGSRLAIWMVPEADLKVYLK
                     ASHAVRVRRIHAREDGDIEAIDQFTKERDAKDRGRYLALYNIDIDDVSNAHLVIDTER
                     WSADQIADIIVDAYHLIPLGDPYGSSL"
     gene            20333..21154
                     /locus_tag="PK105_00330"
     CDS             20333..21154
                     /locus_tag="PK105_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU60179.1"
                     /translation="MEAASENKRVAFVTGATSGLGLAISRSLIDIGFIVYGAGRRKPP
                     LDLPDNYVYVQTDVALDESVNKSRDFVLSQQSHIDLLVCAAGFGISGSIEDTPIDSAI
                     DQFNVNFFGVARVVCAFLPGMREQGRGRIIIIGSIAGKTGMPFQAYYSASKFALEGFV
                     ESLRYEVRRFNIEACIVEPGDFKTGFTGSRQKIIAPESPYYNQFERVISVQEHDEIHG
                     LDPAKLGERIARLAVSKRLPARLTIGPLFERLAVWIRRILPDAWFEAFYRMYYHL"
     gene            21234..22286
                     /locus_tag="PK105_00335"
     CDS             21234..22286
                     /locus_tag="PK105_00335"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOU60180.1"
                     /translation="MHVATSSIEKKHFGTLYSGEEVSLFILKAGSFQASFTDWGATWV
                     SLIMPDKEGQKDDILLGFSTLSGYVGKHPYFGSTIGRFANRIGGARFVLNGIGYKLWT
                     NDNCNHLHGGRKGFDKFLWNYELSAFDGEPAIRFSRTSPDKEEGYPGTLYAEVIASLS
                     SLGELHLHYYAETDAMTPVNLTNHAYFNLAGEGKGTILDHELEMQAGMYLPVDEYLIP
                     NGEICSVEGTALDFRCPKRIGRDIQEVGNGYDFCYIIDKDRLPLKTFAIVTEPSGGRS
                     LSISTTLPAVQFYTGNNLAGTFGKSGSIYDKYAGFCLETEMYPDSPNKQNFPSCILLP
                     GMPWEHETVYKFSLQC"
     gene            22301..23432
                     /gene="prfB"
                     /locus_tag="PK105_00340"
     CDS             join(22301..22369,22371..23432)
                     /gene="prfB"
                     /locus_tag="PK105_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702248.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOU60181.1"
                     /translation="MLEDISKPIEDIKEDILEIWGRLDAPAIEDKIHDLEGLASAPDF
                     WSEQTQAKKILSEIKILRDRLETWQTMHNDILTLEEMYELARQEDQQDMEADLRVSLE
                     EIRTRFDRAVVIEMLSGEADRNDAYLTIHAGSGGTEACDWASMLYRMYVRWAERHDFK
                     VETLDFLEAEGGIKSVTIQISGEFVYGYLKGETGVHRLVRISPFDANARRHTSFASVS
                     VMPVLDESIEIDIRPEDIRIDTYRAGGAGGQKVNKTDSAVRITHLATGIVVTCQNERS
                     QYKNKDVAMNILKSRLYEYYKAERDKETAKFAGEKKEIAWGSQIRSYVFQPYTMVKDH
                     RNKFSVGNVQAVMDGDLDPFLEAYLHWQWKGGTSVEEEDEDI"
     gene            23419..24339
                     /locus_tag="PK105_00345"
     CDS             23419..24339
                     /locus_tag="PK105_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60182.1"
                     /translation="MKTYKDLFLFPLFLFLLIDTSLFAQITPSQNKGLGSIALVFPIQ
                     VSNLPDFDPLGQGNLDSIIILEFIQDRLSDPQYYENKPSPSEANSLSRLMEETLRLRT
                     TSEELIAIDIETLKNWNWNRTIQEKSALFQEGSLKKSDAVLFMMYTIEDSNMQLASFL
                     LKNTGESYAFLSNRFSVFDLLDAARKTVDSFIEKGALLAKKSTPVQSELSIEEIELPS
                     IDPWKNRDNYKKAQSAFETSMGGAAISLSISFVAVGLWNIYREAAYRNSAFDVAATIS
                     AIAAGTSAAVTATFLTISIWNAGKMLGASH"
     gene            24365..25231
                     /gene="ftsY"
                     /locus_tag="PK105_00350"
     CDS             24365..25231
                     /gene="ftsY"
                     /locus_tag="PK105_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOU60183.1"
                     /translation="MNFKDKIKALFHRSQLSEEVFEELEDLLIEGDIGASIAFEVVDA
                     LKDTCRKDNISTPEEARKGLKKILRPFIRVEDFNLDDKRLNIVLILGVNGVGKTTSCA
                     KLAKWAENKGINQPIILAACDTFRAAAIEQLRIHGQRLGARVIAQQHGSDPGAVLWDA
                     IDAAKKNNANLVIADTAGRMHTRADLLRELEKLDRVVSQKAGDVNYRRLLVLDATTGQ
                     NAMRQAEIFNGSVKIDGLIMAKYDSASKGGMIIAVSKQFGLPTVFLGTGEKYNDFEPF
                     DIDKYLDDFVGD"
     gene            25246..26577
                     /locus_tag="PK105_00355"
     CDS             25246..26577
                     /locus_tag="PK105_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60184.1"
                     /translation="MIKTSDEKKQRRMLSGMRFRLPLYIAIRWSITGKKSAHSSGLKF
                     ASLGIAVGVIALIVVLSVMNGLQLQYIESLLETTSFHIRARIEESNLDIFTDTLRKNR
                     LVKSVTPFIETNLLVQSSEDRQNVLRVMWIDPEDLALDSGYCKALNILPSAAAKSLEE
                     GIILGSEAAQILGVSEGSILTLRGAFVDPEEGIQGYSERVKVSSIFRSGYYELDAAFA
                     IMPRLKAKDLAGFPASTILGIKLINAEKSISFVSMLEQEPGIIDIESWQNYNRSFFSA
                     LHTEKILMFLLVGIIFLVVAINIHYSMRRSIARKSRDLAILAAMGMNQHSISSIFMLE
                     GILVGLAGALFGIAIGIPIAQHVDGIINGAIGLIESFISFLYRLGFVKGVPDLRVFSP
                     SIFYIEGIPSRIFISDIAVIAAFALIFPVIAVYLAYRRFRNVSPLEVLRSE"
     gene            26570..27253
                     /locus_tag="PK105_00360"
     CDS             26570..27253
                     /locus_tag="PK105_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60185.1"
                     /translation="MSNPIIECRDLTKTFSSSAEVLCILDRANFAMKRGQICTILGPS
                     GSGKSTFLAILGSLERFDSGDVHVDGYDLSSLSEKSLSMFRRNIVGFVFQFHFLLNDF
                     TALENVALPSYVAGTPKNEAWDRARRLLDMVGLGKRLDHFPSQLSGGERQRVAVARAL
                     INSPSIVLADEPTGNLDASSAKELRSLLQNLPESAGTTLLIVTHDPDLAAIGGSSYLL
                     KGGRFECIK"
     gene            27241..28563
                     /locus_tag="PK105_00365"
     CDS             27241..28563
                     /locus_tag="PK105_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU60186.1"
                     /translation="MHKIRLSGLKPFSYLGFRMLLGRPAAAREKGAHSARHGVFLGAI
                     LGIGISIVPLYIVIFVSNGMIKGITDRYLETKTGHIQMSIPLDLSEENRVQRIETIEE
                     LPGVVSASYEIDGLGLAASTKCSTSAQIRGLDESITSDRGYREYIEIDAGDLFPQKEN
                     EVVLGRYLAKTLGVEIGETVSLMTLRETEGEEILPKLSIFRVAGIVSSGYRELDANWF
                     IIHSRVARRILSSDMAYSYIGIKTKDPYGPDLDDIMNNVKKLYPEALVRPWRDIEQSL
                     FKSFSSTRSMLVFIMAIAGIVAAVNLSSVLTTFVMEHRKEIAVLRSFGVSRKQTMLIF
                     LLGGTVTGIIGCISGSAVGVAISLCINHLLSAIQTLIDFFTKLFSPSSQAITLLNPDY
                     YLERIPISMNLRYIVFILCSGILVSMIVSLIPAFRSSSIAPAELIRHD"
     gene            28744..29889
                     /gene="recA"
                     /locus_tag="PK105_00370"
     CDS             28744..29889
                     /gene="recA"
                     /locus_tag="PK105_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOU60187.1"
                     /translation="MEIQKEREDKLRALEAARLQIEKQFGQGSLMKLGAHTIAQGIEV
                     ISSGSILLDEALGIGGYPRGRIIEIYGPESSGKTTLALHAIAEAQKKGGIAAFIDAEH
                     ALDPIYARNLGVDINELYISQPDNGEQALDIAESLIRSGAVDIIVIDSVAALTPQAEI
                     EGEMGDSHVGLQARLMSQALRKLAGTLSKSKTILVFINQIRMKIGVMFGNPETTTGGN
                     ALKFYSSVRIEVRRIDTIEGGQNEEAIGNRVRVKVVKNKVAPPFRKAELEIIFGKGIS
                     WSASLLDAGVKCNVIEKRGAWYTWSGEKIGQGRENAKAYLEQNAEVAKEIETRVREIL
                     FNTDKSKTTEANTSQTNHSGTIDTFETATILENYEDNDTKIRTEDLF"
     gene            29926..30438
                     /gene="folK"
                     /locus_tag="PK105_00375"
     CDS             29926..30438
                     /gene="folK"
                     /locus_tag="PK105_00375"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOU60188.1"
                     /translation="MEKVWLGLGSNKGDSPSILDSAIQELAGFIIELRRSGLWRSRAR
                     YYTDQQDFLNLAICGFTDLTPRELLIQIHRIEANHGRDRSKEIIKGPRTLDIDILIYD
                     QKIVAEPDLIIPHPGLAERKFALLPLLELDYELIHPGLGVSLQELAACLPPQGIYPVN
                     DNRYDWPYSE"
     gene            30435..31199
                     /locus_tag="PK105_00380"
     CDS             30435..31199
                     /locus_tag="PK105_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOU60189.1"
                     /translation="MTKELDDDSIQEKNSLISNGRTFCLSDFEGPLDLLLYLIKKNEM
                     NINDIRISSITEQFLQCLDSDPSADLDEISEFYQMAATLLYIKSRGLIPHIEDEAEEL
                     DDPRRNLIDQLIEYHRFKKLSELMEQREMEVEWFVERKVAQRTLPFADNPDIDPWIPA
                     DSWDLLRIFASMIRNFSSERIIDLYEEVSINEKIALIYDDLISRNTSAMDLACAFLAV
                     LDSTKNKLISVRQHKLFGDILIIPYSTTEPQNERTA"
     gene            31183..31740
                     /gene="scpB"
                     /locus_tag="PK105_00385"
     CDS             31183..31740
                     /gene="scpB"
                     /locus_tag="PK105_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOU60190.1"
                     /translation="MNEQLDLDAALISAILFLENEPISEEKLAKISGLTLEQTSLALQ
                     RICSEFDQPIHGFAPIRREERWIFAPKLAFWDKLKDHYGKRNEMRLSRAAMETLAIIA
                     YSQPITRAEIEAIRGVNVDAMVRLLLEKNLIAEISKRDVPGKPMQYGTTKEFLRCFGL
                     NSIEDLPKLDEVENSRFSRLETPEG"
     gene            31737..32495
                     /locus_tag="PK105_00390"
     CDS             31737..32495
                     /locus_tag="PK105_00390"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOU60191.1"
                     /translation="MSESIEKPMRLHAYLASLGIASRRSCEEMIREGRVTVDGVKASI
                     GQKIICNQRIEIDGQPIIAAYPKKFRYILLNKPKGYLSSLSDPYGRPCAIDLISGEVN
                     ERIYNIGRLDQWSSGLLLFTNDGDLASKLMHPSGQMEKEYRIQTNVPIPDNFASSFMR
                     GIIIDGVRYRARSVHITGPNSALIVLIEGKNREIRRVLEHFELHTRLLERIRYGPICD
                     PGLAPGNWRELTVDEVQALKTLCNPNQKAKGKGQ"
     gene            32492..34883
                     /gene="rpsA"
                     /locus_tag="PK105_00395"
                     /pseudo
     CDS             32492..34883
                     /gene="rpsA"
                     /locus_tag="PK105_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707864.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
     gene            34916..36430
                     /locus_tag="PK105_00400"
     CDS             34916..36430
                     /locus_tag="PK105_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOU60192.1"
                     /translation="MASEIDSDRFRTLIDINAQLNSSYTNYRSLLKTIVESAARLVGA
                     EAASLALFDASTESLRFEIALGPRGTELQGKSVPAAEGIVGWVFKNRRSIIVNDAAND
                     PRFSPKVSREISYSTNSILAVPLKIKDKTEGVIELINKASHGIFSSEDLKWVELFAVQ
                     AGIAFDNARQYEKTEQELIYLQHKVQQGEGWHPLIFSSGVMRDRLELVKRIAPSDASV
                     LILGESGVGKELIAEQLHLLSKRSDGPFMRVNCAALPESLLESELFGHVKGAFTDAIE
                     NRTGRFEAANGGTIFLDEIAELPLKLQSKLLRVIQQKTFERIGSNETIKVDVRIIAAT
                     NRDIEKLVKNQEFRSDLYYRLNVLPIQIPPLRERLDDVPTLAEYFLKKFTRETNKGIM
                     RFSESAMQSMLSYGWPGNIRELENAVERAVLLAKGQVITADDLMIGSKSSIEDEQFGG
                     KDLREAITIFKKNYIESALERNRWNQTETARNLRIQRTYLSRLIKELNILQNKE"
     gene            36434..37150
                     /locus_tag="PK105_00405"
     CDS             36434..37150
                     /locus_tag="PK105_00405"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU60193.1"
                     /translation="MNQEEMQQAQHNKEEKKSSSERFVDFISKNRRIIIGLGIAIVLI
                     IVAIGIYTAVSGNIASASSRAMELADQKALQWSQETDEEKRAEIESSLIAELDSIAQK
                     WPRTLAAQRALLRKAALLSQKKEYAEAEKTALEAYRRNKKSYVAPIALELAAVAAEEA
                     GNTDAALEHYTLITKDYKKDNPAAAHAFFNLGRIKEAKGDYKGAIEAYNQLIASFGDS
                     EWAMLAKNRVIYLNAQGLAG"
     gene            37242..>37928
                     /locus_tag="PK105_00410"
     CDS             37242..>37928
                     /locus_tag="PK105_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60194.1"
                     /translation="MKGEGKEKGIRFLLQPIAALLIINIYIIMTSCGLPTTDYLYTPK
                     GFSSEGGALVLEHDTKNIDELSSIFLGYEIYYRIFDDEDDAVDSRNSISSMKSSNIIQ
                     TANNYKYFPLKHRKKSESGWYSLEISPLIKVSSNTYTKYYLNMKSQETWTLTDEDLKI
                     VIDSIVRNRSDTASSAEIEYADFCLSSEYKVNDRDYAGSSPPEKAVYIVFFAFAYGFD
                     TSTMRNVYSDP"
     assembly_gap    37929..38028
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(38159..40885)
                     /locus_tag="PK105_00415"
     CDS             complement(38159..40885)
                     /locus_tag="PK105_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOU60195.1"
                     /translation="MNIEFIDPLRDIGEALLKVQKPARYTGGECDAFPPIDPLDSRLR
                     IALSFPDLYEIGMSNNALRIIYAMLNERRDALVCERVFAPARDFEHLLIDKHIPLYTL
                     ESGIPLKETDVLGFSIGYELLATNILAIIDRAWIPLENIERGEGDPIIIAGGPAITNP
                     MPFARFLDAVWIGEAEEGFTELMLKLAELKSRGATRIEKLHLLALHPSIWISPFLQEL
                     GLQPKKKVRRAIYQDFYRTEFVPSFPYPVINPVHSHGSVEIMRGCPNGCRFCHAGYFY
                     RPQRCKSPSIIKKEIEDLIIKKGYTEITLSSLSSGDYPDIVGLFQDLTSSWSKHNVSF
                     QLPSLKVDSFTLPLIEKISEVRKSGLTFAIETPLDSWQCGINKRVPFEKVKSILHEAK
                     LKGFRSAKFYFMIGLPVAERGVEEAEEIISYLNNVASIEHIAIHVNIGTFVPKPHTPF
                     EREGQLSENESLTIIKYMRKKLRSTRNIEISYHSPFLSVLEGLISRGDESIGEIILQA
                     YKQGARLDAWEENLDRNIWNNALLNYNSKKGEGAWEKFLAKRAENEDLPWEEISLKIS
                     SRWLKNEKKKAENNQLTLICNENCTNSCGVCGYENSVVSNMILSKPDAESEDSIIIIG
                     KAKAIEDIDNGSEEIDKSRLIGIIDDLFKDNEDDKSRVRSIVAIKLIGVWRKKDIAVL
                     YPMHDVARAFFRAFKVSGFSAAYTQGYNPQPKFELSPPLPLGVEGENEIIAVWINVPE
                     ENIQKACQFICDYQKQLLELININLPKGLHMNALKPSKFLQQRKKTIGSMLKATKWKY
                     RFGNDSSFSMAVDLLKKYSDFFIPINLDSSSISSSNEIELLEKFPGSAGKSLSLFKIL
                     KENSYEFCLQYQDSDIGSSLADSSMKCFKAKRTGCYGYDENKNSYELLQNMI"
     gene            complement(40882..42201)
                     /gene="rodA"
                     /locus_tag="PK105_00420"
     CDS             complement(40882..42201)
                     /gene="rodA"
                     /locus_tag="PK105_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOU60196.1"
                     /translation="MKFQNFKRLFGDIDYYLLFSCFLLCALGIIAIYSSGIDAEGAII
                     SNEYLKQILWFGIGIFLLIAAALFDFSRYKDIVWVFFAFAILLLIITLLAGRVVNGAR
                     SWLGIGGIGIQPAEFTKVATVLMLARYLESAEFDSSFKKLLITGIIMIIPLALILAQP
                     DFGSALVFVPAALVMLTLADVDFRYILFGVLSIFGVFLFLSLPLYASNHPNPENIIYT
                     AITHENVLIAILIFVLVISIISGIGWLKFKKRYYYWFSYSMAILAISMIGAVAAHSIL
                     KPYQIMRLLVFIDPSIDPKGSGWNILQSITAIGSGGFLGKGFLQGTHSHARYIPQQST
                     DFIFSIIAEEFGFIGCTVLFILYGILFFRCVFLIETCKDRFSQFVIGGLLGIFAFHFM
                     INIGMAMGIMPVTGIPLYFVSYGGSSLWTGLISIGILTGISARRYSA"
     gene            complement(42198..44057)
                     /gene="mrdA"
                     /locus_tag="PK105_00425"
     CDS             complement(42198..44057)
                     /gene="mrdA"
                     /locus_tag="PK105_00425"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOU60197.1"
                     /translation="MAMLPKKITLRMRILKALLISLLSVYSINLFSMQILRKELFVSE
                     AQRISIQSTRIPAPRGEIYDRSGRDLLAGNREAYSVYITPSELPDSKKSDVIKKLSLL
                     LGIPTEDIESKFPDLSLYSYGRVVVAKNASLEAVTQIAARIDEFPGVSWSSKPLRNYA
                     DLRSLSNVIGYVGAITRDEYKLLYNKGYILEDLTGKAGIELEHEELLKGKDGWISKAV
                     DVYGKDISSNAAEIQEPIPGKRLILSIDGNIQRIAEEALGSREGSIIVLKPSTGEILA
                     MVTYPYYDSRVFMSDNIGKAYIDLLNDPKKPMLNRAYQSSYPVASTFKTVLTAGILEE
                     GSISPDKKIYCSGELIYGGRSWSCHIKKPGHGALDLKDALAQSCDIYFWIAGRDYLGI
                     ENIVSYAQDFGYGKATGIDLPAENAGLLPTPTWKEEKYNDTWTHGDTMNLSIGQGYLL
                     ASPLQLANMMAMIVNDGIIYKPHILKEVRDPTTSALIDEIQPEILHQSSISAKTFETL
                     KSYLRDVIVHGTARVPINTKVVQVAGKTGTAEVGLKDRWHSWFVSFGPYDAPAKDQIV
                     VTTMIEASNPWEWWAPYAANVIYQAIYANQNAHDAAKAVGVRLEGSSLIGRRE"
     gene            complement(44086..44595)
                     /gene="mreD"
                     /locus_tag="PK105_00430"
     CDS             complement(44086..44595)
                     /gene="mreD"
                     /locus_tag="PK105_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOU60198.1"
                     /translation="MKSIFISGIVATLMLFIQSTWLSRGIIMGIIPDLALGVLLFCSF
                     INREGQGIIASFLVGIAADILSASPLGYYAFLYTSCGYFATLISYVTEKDIFIVPFLL
                     GAGATIIMALLSKVIYLLFSAGIHYYHIFSMEFGIELMLNGLFTILLFFLLSFIRQLF
                     EHNPKKAMP"
     gene            complement(44592..45470)
                     /gene="mreC"
                     /locus_tag="PK105_00435"
     CDS             complement(44592..45470)
                     /gene="mreC"
                     /locus_tag="PK105_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOU60199.1"
                     /translation="MKTGSQIHGPARAMHKKAGICLVLSIIVLFISTKTIVKVPGFFN
                     GYIIGGIQKGLSAIGSFFTGTIGAVSELRNIERDYEALLEKVQDYELKVRDYTALREE
                     NQRLKELLGLSTLPDAKKIAAHVIARDPTNMYSSLVIDKGYINGIRKYMPVVAYQNGV
                     EGLVGKIIEVKASTSILQPLYDQRFFAAARLAKTRAEGMLNGQGFRDVPLNLLYVPKA
                     EADLLKRGDIVVTSGLDEIFPSEIIIGRVEDWRLNELSSSLVISVQPAIDLSRIEYVF
                     AIDIEPLSSPKKEAIQ"
     gene            complement(45487..46506)
                     /locus_tag="PK105_00440"
     CDS             complement(45487..46506)
                     /locus_tag="PK105_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOU60200.1"
                     /translation="MFSPDIGIDLGTCNTLIYMNGKGIVLDEPSVVAVERGTNRIVAV
                     GTEAKSMLSRTPTDIIAKRPLRDGVIADPDMTEKMIKYFIQRVFPKNLFFKPRIVVGV
                     PTCITKVEENAVIDSTYKAGARMVKVIAESLAAAIGAGIPITEPAGHMICDIGGGTTE
                     ISVISLKGMVVTNAIRVGGDEFDEAIAKHIRNIHNLIIGEQTAERLKIEIGNANPERT
                     IEKIEIKGTDAITGLPRRFEVDSVEIREALMDSVNLIVEEIKKTLGKTPPELASDIVE
                     RGIVLSGGGALLKGLPKLIAKETGVPVILSEHPLECVAIGAGKYFEIMQKNIGSSSVY
                     ESLNL"
     gene            complement(46538..47479)
                     /locus_tag="PK105_00445"
     CDS             complement(46538..47479)
                     /locus_tag="PK105_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU60201.1"
                     /translation="MLDRFFRGKAHHTKKKNPWLFIAIALIAVGVFLGFYFAIRGRSA
                     KNSISRSALAKVWNSGNNIEQTLSEAQKAIESYPFDEYYLSICGIAYYYSALNAQEEE
                     IRQQELEEAVIALRKALAVGLPSRMKGQVYYILAKAYYQKGEPWFDLAERYFLLAKES
                     GSKEKDIPQYLAIVYAGRKDYSRAVSWYELALENNTSDALLLSAAISYMNTDQIEKAR
                     DLLLRLESSASDAKIRLKATLLLAQDNFDSGDIQSAQDRYQGVLKEDPVNVDAWYGLG
                     LIYAKLNDQLGARAAFRKVVQIDPNHADARKRLAEKL"
     gene            complement(47496..47908)
                     /gene="rnpB"
                     /locus_tag="PK105_00450"
     ncRNA           complement(47496..47908)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK105_00450"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(47917..48840)
                     /locus_tag="PK105_00455"
     CDS             complement(47917..48840)
                     /locus_tag="PK105_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOU60202.1"
                     /translation="MPMRMDDLFETLREYQELLSNRVELEKQLEAMPKTIEIQNQMLS
                     REKKSLVDAKEQFTTTEANLKQLRQALAEAEMRRENAERQMDGIQTQREYDAVNKEIR
                     EASEKEQDLRHRIQEEERRFAEIDDRLHRIEISVSALESEIEQKRAEIDQNSKEIIEE
                     IENLRTKENSLTQELEDDLMFKFERIIRSKSGMGIVPIHGVVCTGCHMILPANFVNEV
                     RQADKIVFCPYCSRILYYEETEIEGSIEEILTGSLVDLGDEDSEDADDAESEDESEDT
                     SDSSKKSSSKKRKNQTEPLIDNDFLIDNMEE"
BASE COUNT        14130 a        10080 c        10782 g        13782 t
ORIGIN      
        1 ttgttcacta ttgatgagcg tatcgaaatg ctcaatgcca tgaacagatt cggtgaacaa
       61 atgaaggtgt gcaaatggga tggcctggta accgattttg ctcgtaaaca tggatgctct
      121 gtgctcgtgc gcggactccg caatgctgcc gagttgccct atgagaccac tatggcttac
      181 atgaaccaaa gacttgaccc ttccataaag acagtttttt tcctctatga tgctaagcat
      241 gtggatataa gctcaacact tgtgcgcgat ctcgtaacac ataacaggct tcctgatgat
      301 atcgtaccac aagaagttga aaaggttctt aggcaaattt tgcagagaag agggtgccct
      361 ctttcataac ttgcacaaat gatatatgtt taaccactgc aagcatgtct atcttgacat
      421 tttctcacat tttgtataat ttggcatgca attatcgtgc cgatattgaa tgattatgag
      481 gttctactat ggctgtaccg aaattcagga cgtctaaagc aagaacgaga agacgccgct
      541 ctatcaacat gaaactaagc gcaccaactg tcgtacattg cagtaattgt ggcaacccgg
      601 tgatgttgca tagggtttgc ccaaaatgcg gtttttacag gggtcgtcaa gtcattaccc
      661 ctgaatctaa ataaggagtc tcccaatgga tgagctgttt gagaagatta agaagattat
      721 tgctgacaag ctcgaagtag atgaaagcaa gatcacaatg gatgcaagtt tccgccagga
      781 tcttggtgca gattctctag acacctatga gctcgtatat ggtatcgaag aggaactggg
      841 tattcagatt ccagatgaga aggcgaatga atttgaaacc gtaaaagatg cctatgagtt
      901 cataaagacc caggtaaaat aagacattct aaggcgtgag acgaaaagat cacttaccat
      961 caaacaacag gctggagatt tcgtcttcac gccgcagtgc tctactgcgc ttccagagag
     1021 ccacagatat ttcttttcat gatataacac tccttgatat agcccttacg cacagctcct
     1081 cagtaaacga aaaatctcca tatatacagg acaatgaacg cttagaattc ctgggtgata
     1141 gtgttttaaa tctatgcata tctcatatct tatatgaaag tttgcctgac aatgatgaag
     1201 gcgaattagc ccatatgaag aatgtcttgg tatcggaaac ttctctagca tcaatcgcaa
     1261 tatctctcgg tattggggaa tttcttatcc ttgggaaggg agaagagatg agtggagggc
     1321 gaagaaagcc agctattcta gccgatgcta cagaagcact tcttggtgct tactacctgg
     1381 atagtggaat acaatctacc aaggaaattg tttccaggat ctttagtagc aagattgatg
     1441 agctccgaaa aacaccaagc aaggacttca aatcaataat tcaagaatat gctcaaaagc
     1501 gtggactaga aattccttcc tatagggttg cgagaatcga agggccagac catgctcggc
     1561 atttttttgt atcctgtgtt atagatgggg aaacctttgg ccctatggaa ggaatgtcaa
     1621 aaaaagaggc cgagcagcga gtcgctcagc atatatttga ggtattacat aaacgagatg
     1681 cagagactgc cagactctta gacatcgtga caaaaggcag cacagagatc gatgagaaga
     1741 tatagagagt cgatctctta tttgaatcct tcaaaatatc tgccttttgc tatttctagg
     1801 agcttttctt tggtgttcaa agatgggtct tcgagcacat tatctaagag ctcggataga
     1861 acttctccca tttgtttggc gcgcggaatc ccgatttttg cgagatcttc tccatttatt
     1921 gccagatctt tgagcgaaag agcctgtttt tcttccagaa ttcgatctat tctctctcgg
     1981 aattctgcta gagcaggcca ggatatgggt ttccctgtag ttgccattgt atcggctaat
     2041 ctaagcgtaa acagatcggg aatcgaggag aatcctacgc gagcgacaaa tctacgtatc
     2101 gctgcatctg tccaacctgg ttcataattg aacatatgat aacgcacaag gtgacagacc
     2161 ttatcgataa ctcgatttgg atacttgagt cgtagaagaa tttgccttgc aattttctct
     2221 gattctattt catgtcgata aaagcttatt ttcccatttt cagctcgata acatgcaggt
     2281 ttgccaatat cgtgaaaaag ggctgaaaga cgaaggataa ggaccttgtc ttcgcttaga
     2341 ttatctggaa tgacagcatc cacggtcgaa agcaggtgat tatagacatc ctgttctcca
     2401 tgatcaggtt gagaaattcc tttgcatatt tctaattccg ggattatttc ctttatcagg
     2461 ttcgtatccg aaagaagtcg aaggccaacc gaaggctttg cagtcaataa gatcttggaa
     2521 aactcatcgc gaattctctc tgtggaaatt ttcgagagag tgtccgaaca cttagctatg
     2581 gcaacaaggg tattgggttc aatggcaaac tcgagttgag cagaaaagcg tattgcgcgt
     2641 actgttctaa gaccatcctc ctgaaatcgt tccattggat tgcccactgc gcgaataatt
     2701 ttatctttca ggtccttttc tccgtcaaat ggatcaatga atgcattgca catcagatcg
     2761 atggccatcg cgttaatggt gaaatccctt cttgagaggt cctgggttat gtcatcgaca
     2821 aaggtgacgc tatcggggtg tctgccatca gaataggcag cttcttttcg aagcgttgtt
     2881 atttccacct tatatctacg tgaacgagga acaatagtta tggtgccatg ttttataccg
     2941 gtaggtatgg caaaagggaa taaactcatg cattcttcgg gtgttgcatc ggtcgcgata
     3001 tcgtaatcca taacgggctt tctaagaaat gcatcgcgga cagcaccacc gaccaggaat
     3061 gcctttttac cactttggat tattctgtct gcgattctgc gaacctctgg atcgatacta
     3121 gggattatca tatcttcctt tggagtgtat cttaggcttc agctttatgt ctatgaggaa
     3181 actgatatga aagctcttat tgtaactggt ggcgataatc ctccctcatc cataatttgg
     3241 tgcctttcca agaaagccga tatcgtaata gccgcagatg ccggacttga ggtatgcaaa
     3301 atagccaata tagagcctca tctagctgtg ggagattttg acagtgtgga tccagcaata
     3361 ctatctcagg tttcaccgga gaagattctt cgttttccgg aagaaaagga tcgttctgat
     3421 accgagctag caatcgaagc agcgcatcgt tacggagcaa aatacataat cctcgccgga
     3481 gctggagcag ggcgtcttga tcatctgctc gcagttagag ctcttttcga gcgcaacgac
     3541 cctatcaaag agtggcatac agcacacgag agtgtttttc tcgttgctcg agggcttagg
     3601 ctgcaggcct caatgtcaat taatagtatc gtttctgtct ttcctgtctc cggaggagcc
     3661 tctggcatgc tatctaaagg actcaaatgg cccttagatg gcttgaagtg gaagcgcggc
     3721 gaatttggcc taagcaacaa gacaatctcc gaagaaatag agatttattc tggtgagaat
     3781 tcaattctcg taattctacc tcttggttcc aaagtgtctg tctcatagaa ttgctagatt
     3841 cccgaatatt tctcttaact gcaggagggt atcttttgga tcctggacta ggacgcgaga
     3901 tccagctatg agctccacaa tgcctatttc tcgcggataa cgaggtataa tatgcatgtg
     3961 taaatgttct atgctggctc ctgcagataa gcccatattg tatccaatgt tataggcaga
     4021 ggggctcccg aatttatcga gcgcttttag acatgttttg actaatctat ctagttctct
     4081 ctcttcgtca tcagtgtatt cccttatatc cacaatatgt cttttgggaa agactatcag
     4141 atgccctgga ttataagggt aaagattaat ggatacaatg aaatgcccag tctcatctac
     4201 tacgagtttt tccacttctt tagaatcgtc gcggacaagg cagagtatac agccttttgg
     4261 tcttctacct tgtagataac caattttgtt gaagttaaag agatattcca tatggaagta
     4321 tgatatatta gcattatgga aaacaaaagc ggctcgcaat tacaatccgc ttttataccg
     4381 actcttaagc ggtttctaga ttataccgtg gtttttagta tcatcttttt gataagtagt
     4441 ttcgttgtgc ttttctttgg cggaattagt gaatatgaat ggatttggcg atcaatattt
     4501 gaaatatgca atatgcttct tctaagtgtg agtctcttca atatgacaag catggtaggc
     4561 ttaaggatag ctgggaagga tcgtttctct attgggcgtt tcattcttca gctccttctc
     4621 tttgtttttg cagtagcagc tctaatgctg aatatcttcg taaaaatcac ccgaatctgt
     4681 tgaattttgg ttgccagatg gattttgcga ctattggagc tgattgtctt taattcttgc
     4741 atagaaagag atggaaatgt atagtaattc aaatctatgc aatgttaagg aattttcagc
     4801 agagattgat caggaggaat aatgtccgtt agagtgcgct atgcgccatc ccctacagga
     4861 aatcaacata taggcggcgt aagaacagcg ttgatcaact atctattcgc gaaatcccaa
     4921 ggtggaactt ttattttgcg cttagaggat acggatcgaa cccgttattc acaggaatat
     4981 gttcaaaatc tatacgatac attcaggtgg cttgggtttt actgggacga aggtcctgat
     5041 gtcggtggcc ctaagggacc ctatgtgcaa tctgaacgaa tagatctcta tcgaaggtat
     5101 gcagaagaac ttgtccttat ggggaaggct tattattgtt tctgcgattc agagcgcctc
     5161 gagagacttc gtctggagca gatctcacag aaaaaagacg aaataggcta cgatcgccat
     5221 tgtcgtttta tcacagaaga agaaagagca aaaaggctcg cggaagcaac accttatgtg
     5281 atcaggctaa aaatccccct tcatggggta acggttttcc acgatgctct tcttggtcaa
     5341 atagaatgga aaaatgagga tataagccca gacccggttc tattaaagtc cgacggtttt
     5401 ccgacctatc atctggctaa tgtgatagat gatcatttga tgggaattac aaacattatg
     5461 cgtgctcagg agtggattcc ttcggctcct atgcacaaga tcatgtatga tgcctttggt
     5521 tgggaaatgc ctgaactctg tcatctgcct atggtactgg gtaaagacgg tcacaagcta
     5581 tccaagcgac atggggctac ggcagtgaag gaatttcgga aagctggcta tcttcctgaa
     5641 gctctcatca attatatcgc tctgcttggg tgttcgtatg aagatggtcg ggatttatat
     5701 agtctggacg agcttgttcg tctattcaaa atagacagac tcaataaggc acctgcagta
     5761 ttcgattatc aaaaactgga gtggttcaat ggtcaatata tccgcatgaa aagcgataaa
     5821 gagctagccg agcttattcg tccatatttg atcgatgctg gcttgagaac tgaagaagac
     5881 ccacaaaagg accggatgga gctggccgca ataccattaa tcaaagagag actaaagctg
     5941 cttggagatt cgcctgcgat catgtcctat ctctataaac gactggaaat gcctccggca
     6001 gaggcattta ttcccaagag agccagcatg gacgaagcaa taatgtatct tgaggaatgc
     6061 agatcgatca ttcaggattc taatctcgac gacattcccg ccgttgagga gagatttaag
     6121 gaatttgcta cagtgattgg aaagaaactt ggagacattc tgatgcctct aagggttgcg
     6181 atcacttatt ctcgtgtcag cccgccacta tttgaatcga tgcggattct tggaaaagag
     6241 gaatgtattg taagaatcga ggctgcaata gcatatctgc ggggagcttg acctaactat
     6301 tcaatccaag cgatattttg tttactgtgc ctctgaagcg cagcaaggag acttgtatca
     6361 tgtctgaatc aattgaaaag cctgaatatc tggattttat ccgcgaggca gttgctgaag
     6421 atttgcggat cgggcgatgc aaagaagtcc gtaccaggtt tccaccagaa ccaaatggat
     6481 ggcttcacat aggtcatgcc aaagcacttt ttgtggattt tggtgtggct caggactttg
     6541 gtggtaaatg caatctgcga atggatgaca caaatccaga aaaagaagat atggagtatg
     6601 tagaggcaat caaaagagat ataaaatggt taggcttcga ctgggaagac aggctttttt
     6661 ttgcttctga ttactatgag agactatttg agttggcctg taaattgatc aataaaggcc
     6721 ttgcctatgt cgatgatctg gatgaagaac agattaaaga atatcggggc acgaatgtgc
     6781 cggataagaa taatattacc tttactcctc ctggtcgaaa cagcccatgg agagatcgtt
     6841 cccccaaaga gaatcttgat ctattcgaaa gaatgcgagc cggcgaattc cctgatggtt
     6901 caaagactct acgcgcaaag atcgatatgg ctcatcctaa tctcctcatg cgagaccctg
     6961 ttatgtacag aatcagaaga attcctcact ataggacagg aacaaggtgg tgcatatatc
     7021 caatgtacga ttttgcacac ccactctccg acgccatcga gggaataacc cattctttat
     7081 gttctctaga gtacgagatt catagaccct tgtatgaatg gtttattcaa aactgcgagg
     7141 tattccctag tcgccaaatt gaattcgctc gtcttaacct atctcataca gttctctcta
     7201 agcgctggct gcttcaactt gtccgaggga aaaaggtcaa aggttgggat gatcctcgca
     7261 tgcccactat tgccggcatg cgtaggcgcg gatatcctgc ggcagggatc agggattttt
     7321 tggggagaat tggtattgca aagactgatt ctctagtgga tattcagctg ttagagcatt
     7381 gtatacgcga agaattaaat gcacatgcaa atcgttatat ggctgtcatg aatcccgtaa
     7441 agcttgttat agaaaactgg cctaaaggaa aaatcgaatg gcttgaagcc atcaacaatc
     7501 cggaggatcc ttccgcaggc ataagaaaga ttccttttag tggcactctc tacatagatc
     7561 gagaagattt taaagaaact cctccgccca aatattatag actctatcct ggcaatcagg
     7621 ttcgattgcg ctatggttat gttgttactt gtacgggttt tgaaaaagat ccgatcagtg
     7681 gagaaattga gagaataaga tgtgtctatg atccaaatac aagaggcgga gacgcacccg
     7741 acaatcgaaa agtaaaagga actatccaat gggtctgtgc catgcatgca gttccaattg
     7801 aagccagatt atacgatcat ctcttcgatg cggaacgccc catggaagtg cctgaaggca
     7861 agactttttt tgataatctc tctgaccact ctcttataat cgaatctaga gcggtggccg
     7921 agccagccat agccgaaatt ggaaaaggcg agacagtaca atttgagagg ataggatatt
     7981 tttgcaagga tctcgaatca acaagcgacc ttgccatttt caatcgtacg gtcactttga
     8041 aggatacttg ggcaaaaata gaaaagaaac ttcagaggaa tccttaataa tttcttgatc
     8101 ctcaatgatt aagcaatcat attccattaa aggagtaaaa atctatgtca gagaccgtgg
     8161 taactatgga aaagattgtt tccctcgcaa aacgtcgagg ttttgtattt caatcctcgg
     8221 agatatatgg aggtctttcc ggggcttggg actatggccc tcaaggaatt gagctaaaga
     8281 ataggatcgc tcgattctgg tggaaggaaa tgactcagat gcatgataac atagtaggtc
     8341 ttgatgcggc cattttaatg caccccaggg tatgggaagc ttccggacat gttgaaaact
     8401 tcaccgaccc ccttgtcgat tgcaaaaaat gcaagagtcg ctttagagcg gatcaaatcc
     8461 cgcccgaaaa tcttgctgcc aaaaaatgcc cggattgtgg cggcgagctg accgatactc
     8521 gcaaattcaa ccttatgttc aagaccactc taggcccagt ggaagatgaa tccggccttg
     8581 tatacctgcg acctgaaacg gcacagggaa tttatgtgaa cttcaagaat gttcagcaat
     8641 ccaaccgcat gaagattccg ttcggcatcg cgcagattgg aaaagctttt cgcaacgaaa
     8701 tagtcacgaa aaattttata ttcagaacct gtgagtttga acagatggag atgcaattct
     8761 ttgttaagcc tggtacggac gaagattggt tcgaatattg gcgttcagag cgatggagct
     8821 attaccaaaa aatgggcgtg aatatggata aattgcgctg gcataagcat ggacccgatg
     8881 aattagctca ctatgccaaa gatgcttatg atatcgaata cgagtttccc atgggcttca
     8941 aagaactcga aggagtccat aatcgtacca attatgattt gggccgccat cagcagtatt
     9001 caggcaagga tctggaatat attgatcaag atagtggaaa cgaacgctat attccataca
     9061 taatagagac gagtgctggt ttgaccagac agcttctaat gctgctatgc gattcttacg
     9121 aagaacaaaa aatatctgat aaaggtaacg aagatgattg gcgcacggta cttcattttc
     9181 atccggtttt ggctccaact acggtcgcgg ttctgcctct catgaaaaag gatggcttag
     9241 ctgaactagc ccaggatatt cgcatcgaac taaaagatga ttttgtaact gactatgatc
     9301 agtctggtgc cattggcagg cgttatcggc ggcaggatga aattggcaca cctttttgcg
     9361 tgactatcga ctatcagaca aaaaacgata atactgtgac agttcgggac agggacagca
     9421 tgctccagga aaggatatct cgggctaatc taacctcata catacaacat aaaataaaaa
     9481 attatcatgc tcccaataga tcggaagtag cttgtagcta atagctaaag ccaaagatca
     9541 taaaatttcc ctctaataat gatatttcag ggaggttcta tgtattctaa tgattcacta
     9601 attcaaatct aaatcagcaa atcaccactt cctatttgaa gtggtgattt gccgtaactc
     9661 taaggatagt tcactcgcct agaacggcat ccttgcaggg tttggccaca gtaatcttga
     9721 taacttcccg agctggaata gtaatctgtt ctcctgttgc cggattgcgt ccaaccttgg
     9781 ctttgcgatg gacaactttc ataatcgcta tgcccgggat ggtaaacttc ttgttcttct
     9841 tagcttctga ataagcgagc ttagcaaact catccagcaa aagattgaca tctttcttag
     9901 taaagcctgt ggactttgct aatgaatcaa taatctcgac cttggtcagt ggttttcctg
     9961 tcgatggcat attcttcccc tttttttcga gaatggcgac tcagcataat aaggagccgt
    10021 cgcaaatatg catgaagcat attcctgttt ttattttacc acaattatgt tccttgtcta
    10081 gatttggtat tgaaaacttg tagtatttct tactttttgg aaggaataat cgctttttgg
    10141 cgagtacaac aatagaataa cttgagcaag ttggcaattt catcctatac tgcaaaactt
    10201 aaaggagcat ttttcatgaa cagagcgctc gagatcttaa aagaacgcgg ttttgtgcag
    10261 caatgcagtg atttagaggg gctttctaag ctaatggacg aaaggccgat tactttctat
    10321 ataggcactg atccaacggg tccttctctg cacatagggc atatggttcc attttttgca
    10381 atgcgccatc taataggtgc tggccacaga ggcatagcgc tcttaggagg tggaacggcg
    10441 cgcattggag acccatcggg taaggctgag atgagaaagc tcatttccta tgaagaaata
    10501 gatgctaata ctgagaggtt taaggcgcag ctcgataatt tcatccattt cgacggcgac
    10561 catgcgctga ccgctaataa taaggactgg ttatccaacc tcaattacat cgaatttttg
    10621 cgtgatatcg gtcgccattt ttctgtgaat aggatgcttt cctttgaaag ctatagaatg
    10681 cgactggaga cagggctttc ctttatcgag tttaactatc aacttttgca gagctatgat
    10741 tatcttcagc tccataagct tcatgattgt cgattgcaga tagggggcga cgatcagtgg
    10801 ggcaatattg tggccggaat tgaattgata agaagagtag agggtgccga gtgttttggt
    10861 cttacttttc cactggtaac taccagcgat ggcagaaaaa tgggaaagac cgaaagaggc
    10921 gcactatttc ttgatccaga tatcacaagt ccctatgagt ttttccagta ttttagaaat
    10981 gtgccagatg gcgatgtaga gagattcttg ctaatgttta cttttctgcc tatagaagaa
    11041 tgcagatatc taggttcact caaggacaaa gcgcttaacg aagctaaaga gcgacttgca
    11101 tgggaagtaa catctcttat ccatggccaa gaagaagcgg ataaagcgct aagtgcagcc
    11161 ggggcagctt ttaagggaga aggagacgct tcacaacttc caactatcga ggtcgaaata
    11221 tctcggctgg attcaggcat tggagtgctg gatctatttg tagaagcagg acttgcgcct
    11281 tctaagagcg aagcgcgcag actagtcaag caaagcggcg cctcggttaa tggcagaaaa
    11341 atagaaaacg aaatgcagac cttgacatcg agcgatttaa tagatggcgc actcatgctc
    11401 aaagctggga aaaagcgtgt tgcgcggata gtgccaaagt cataagaaca gcatgcagca
    11461 cctatttctt gcacttgcgg atattcatgg caatatcaat gctctggaac agatactcaa
    11521 ggcaaattcc aacatagaag gaatttttgt agcaggagac cttacgaatt tcggaaaaga
    11581 aaaagaagcc caaagagtgc tctcagcttt ttcgagcatt gcacccaagg ccaaactcta
    11641 ttttttggcc ggaaattgcg ataccgcaga agcaaggaag tgctttgata tgcaaccagg
    11701 ctatattgaa cagcgatgct gcgcccttct acctgagaag gagatatgga tcattggttg
    11761 tggtggaggt ctatatcata ctggcctgac acctttcgaa ataagagaag aagggctgga
    11821 aagcggaata aagcaagccc ttaaatgcgc tattcgaggt tatagagata tttcaagtat
    11881 ggatggttca gagcccactc caggtctcat tgttttgact cacactccgc ccaaagacac
    11941 cttttccgac atgcgtcatt cgcgtcatat aggaagtgca gctttccaga cattcctgta
    12001 tgagcaggag cctcttctat ggatttgtgg ccatatccat gagggtaaaa gcatccatat
    12061 tgaaaataag accttagtgg ttaatccagg gccggctgcc catggtttgt attccctaat
    12121 cctcctaaaa aaatctccta agggctggaa agccgcagca gaactaagat cgatatgata
    12181 ttctcgccat ttatcaccga ccggtagcac tataaagata tttgaagtag tccatccccg
    12241 tagacagcgt cttattaaat gcaggcattg tcttgcgata tgactcgatt gctttccaga
    12301 attcgaagaa tgatggatct ctgccaaagg aatcggcgta gatcttcgaa gcttcggcat
    12361 cggcctttcc ttttatagat tcaccttttg catacgcaga agacaaaatg gtgcgctttt
    12421 cattttccaa tttgccgagc cattcggctt tcttcccctc accataagct ctgaacgctt
    12481 gtgctatttg atttctttcc tttatcattc gctgatatac actttcgcta agttcatcgg
    12541 agtacttgat ttgccgtgga aggatatcca caacttcaat accaaaagaa ggaactatgt
    12601 ctgagacaag cgctctcatt tcttctgtca gttccaggcg ccccttttcg attttttcgt
    12661 attgcgttgg tgttattgcg agctgctgaa gagttgcact gttttccacg tctcctgttt
    12721 gaaaactctc tacctggttt tccgaaagaa tttcgtcgct gctacgaacc gcctctcgaa
    12781 gataattcga agagataaca gttctcaccg ccgaatcgat gacatcgcta agcttgctat
    12841 atgctgcgtt caggtcattc acagattcat agaattttat tggatccgca attctccagc
    12901 gagcggtagt atctacatag ataaattgat tttccttagt gggaattctc tggggttctc
    12961 catcccagga cataagtctc ttaggatatt ttatgacagt atcgataata gggctgcgcc
    13021 attttaatcc ggcatcgcta tgagagttga ctattttacc aaaacgtact actacggctt
    13081 gttcaccctc atttagcaca aaaagtggac caaagatcac gatgaataaa atagctaatg
    13141 ccacaacaac cgttattatt attgatgctt ttttcatctt gaagcctcct gagaagaagg
    13201 agtctgggct agattcttta ttggcaagag attctgaagc gatttatcga ttaggtccac
    13261 atgctcttgg tccttaaata tctcctccat catttcgtaa tatagacgct ttcgtgttac
    13321 ttcgggcgcc ttgcgatatt cttcatagac agaattgaaa cgagagacat caccctttgc
    13381 cacattgaca cgctctgaag catagccttg cgcaacttct agtacttggt ccgcttgacc
    13441 ttgtaccttt gggatttctg cgttatatgc ttctcttcct tcattgatga gcctgttcat
    13501 atcctggatc gccttgttca catcttcaaa ggccgcttga acgccttccg gaggtacaat
    13561 gttttgaagc tgaacttgtg tcaccgtaat tccaagacca tatatcgaaa agaattcgtt
    13621 cataagggta accgcggttt cctgaatact ctggcgctca ggccctatga ctcccagaat
    13681 ggtcctatca ccgacaagca tgtttagcac catctgagaa gtatctctta ttgttttttc
    13741 tttatccatc acattgaaca gccaggcctt tggatctgta atacgatatt gtatgatcca
    13801 ctcgacatct acaatattga ggtctccagt caacatcgtt gattcatctg ggaatttttc
    13861 gttcgagtat tgggtaatga tgcctggttt tacggttcta aagccgaaag cttcggtttg
    13921 aaccacctga gtttttacca gatgggcttt ttgaattcca aagggcagct tgtaatgaag
    13981 tcctgctcca agggtctttg tatactttcc aaaggttgta atgacagctt tttcggtttg
    14041 gtctactacg acaaagcttg tcagcaaaaa gactaagact gctaaagcta caattatgat
    14101 tattacaatc ggccatttta gttttacatt cagttttttc ttaggattga taatatctgc
    14161 catgttttgc ccccttctca ggaaatagat tattgtcaca tatttctaat gattgttcta
    14221 tcctatgaga aatcagagca tcaacttcct catgatctat ttggaacaaa tcggaaacag
    14281 caaggttaaa atcaccagga agagggtcaa aaatagtcaa aggcatgtct tcaaaaatag
    14341 gttctggaaa gcctaaacaa catgaatgca aaaaataact gctgattttg gctgtcttag
    14401 atcctccata ttttgaatcg ccgacaagcg gatagccatg agatgctagt tgtgctctta
    14461 tttgatgcgt aagtcctgtt atgagttcga taagcaaaaa actatatgac ttattatgca
    14521 tcaatggaga tagataaagg ctcgcttctt tgccttttct atcatctaat aggctcaatt
    14581 ttctcttttt atcgcgcaga ataaaatcct ttaacgagat aggttcatct atctttcctt
    14641 gaactattgc agcatagaac ttgcgaattc ttctgttctt cattgcaaca ctgaaaagtt
    14701 ccgcgccttt cctggttttt gagaaaaaaa tgattccaga ggtgttccta tccagtcgat
    14761 gcagtggtcc tggagaaaat gacgcagatg gctccaattg atcatagagg attctctgca
    14821 cataagcatt gagactagag cttccatcgt gtaccaatat tcctcgcggc ttatcaaaag
    14881 caataagttg ggcattctgc cacaagatgc ggggaatttc ggaaaaagcc acctgtgaat
    14941 attctttttt aggaagagta ctttttatta tggtttggtt ttgacctttc tccagacttc
    15001 tttcggagaa ctttatttcg atgacatctc cttcttttag tctatcattc ggcgcaatag
    15061 gaatattcga aatccttata tctccctggc ggaaaaggcg atagattgtc gaaagagccg
    15121 tatctttgag cacctttctg actattctgt cagcgcgccg tccatcgtca tccctgctaa
    15181 gggtgatgat caacaatttg cccacgaatt gaccataatg ttttggagct ggttggtcaa
    15241 cgactgccta tgcttcaatc atgcttgaca gagccctaag catcttacac actctacata
    15301 atacgtaatc aggctatata gttcttaaat gccagaggag tatatttctt cgatgcagaa
    15361 cttcaaagtg gtaataggta tgtataataa tctgccctat aatgcgtcgg gagcattgta
    15421 tgaacagaca tatcaaggcg cctggcgccc ttttctttcc ggactataca aatttctctc
    15481 tattaaatct gcaatctact tttctggtgc tattttccca tggcttgaag agaaccatcc
    15541 cgaatatatg taccttctcg tcgagatggt tagaagaggt caggtcgaac ttttaggagg
    15601 cggattgtac aacccgcttt caccccttat aagcgcgcaa gatatgaccg gacaggtcga
    15661 aagtctaagc gcttttattc gcaagacaac aggcaaaagg ccttctggcg catggctcta
    15721 cgaatattca tggagctctt cactgccatc cattctacaa aactcaaaaa tacaatatac
    15781 ttttttgcct gcggagtact ttcccaacgt agagggaggc atggaatttg gcttgccggt
    15841 tacttcggaa gatcatcgaa aggtaataac catatttccc actttcgaat ccaaggactc
    15901 agaaaacagc tttatgcctt atgagaatac tctgacggat cttcaagagt cgtatcctga
    15961 atgcaagact ttcatcatta tggcggatgg caacgaaata agcatgagtt gggaaaagag
    16021 cggccttgaa tctcctgatg ttcttttcga gcgtaccttt gcatggtttc agaagaattg
    16081 tctagaatat gataccctta ctcccattca actccataaa accacaaaat cctctagaat
    16141 agtatatttc tctcaatgtt attctcggcg gtttaaagaa tattgcaaaa agacgatcaa
    16201 tcgcttgcct attcaaaatc aagaatatat acagatttct aaacaatctg tattagatca
    16261 tccacttgtg catgttcttt atcaaaaatt gaactatgta tcaactatga tagctctttt
    16321 tcgaggtgat aaatcgagaa agaaagcctc tatagatgat atctggaaag ctcaatgcgg
    16381 tgatctgttt tggatcggac cctgcggtgg aatccttatt ccggaagcta gaattgcggc
    16441 tttttcctct cttatagagg ctgaaaagac aataaggcag agtagatttc gcccttatct
    16501 atcattcgac gacctcaatt tcgatggctt aaaggaagca atatttcaat cttctgttta
    16561 caactgctat ctgcaatcgg ggtccgcctc ggtaagcgaa ttcgactcca taaagacagg
    16621 aacaaactat gcttgcggtt ggaatgacga tcaattctcg acaggctgct tcaaagatta
    16681 cataaaaacc gaggggaact tcgaaagaga tcttatagca gtagagcgtt ggaatatggt
    16741 agaaaatcca agagaagagt cgagcgttct ttttcgaaga gaattttcgg ctagatttga
    16801 agaccgatta ttgatgcttc tttgtagaaa gacttatcgc ttcagaaatg actttttcag
    16861 tatcgactac gaattgagca ataagaatac ggaagcttgc ctatttcgtt tttgtaccaa
    16921 ttcagagatt atagctgctc cgatttttga ggatcatcga tttgaattgc tgcatcatcg
    16981 cgagagcaag gccttagatt tgagatctca agcttttttc gaaacggttg atggtatcgg
    17041 gcttagcaat gttagaaaag cagaaagagt gttgattcgt tctgatttgc cattctccct
    17101 attggcaaaa cctaatttcc ttaagaagcc ttcggtctct ccgcaagtta tggatgttcc
    17161 atcagattct ctcatgttcg aaggcttttc gatcggtatc ggatgggacc tgtctattcc
    17221 tgctgaggga acagcctttt tttctctctc tgtccatcta gaacattgag gaattagccg
    17281 atatatgtat cttcgattta gcgaggtctt gaggttttag gatgaatttc tttaaaatag
    17341 gagaataatg gagatgattc aattccccga taagctggaa ggccttcata ttcaccttgt
    17401 gggagcaaaa ggcaccggga tgaccgcact tgcggagata cttcaaaagc gaggggcaat
    17461 ccttacgggt agcgatgtgg cagatgtgtt ctataccgat agaattctac actcgcttgg
    17521 tattgagctt tttgagaact tcgacaagaa gaatctacct atcagcactg atatagtcat
    17581 tcactccgcg gcatattcac gcgaaggaaa cccagagctc aaggcagcca cgcatcgtgg
    17641 tatacccatt ttcagttatc ctgaagtact tggagccctt tctctgcata gtagctctgc
    17701 aggcatagcc ggggttcatg gaaaaaccac gacagctgct ttggctggga tactcataaa
    17761 agcattagga ctgcccgcaa cagtgctggc aggttctgcg atttctaatt tcgacgatcg
    17821 atgtactctg gtccaaggag agagcttttt tatagctgaa acttgcgagt accgcaaaca
    17881 ttttctcaac ttcaagccta cttggatagt actcacatcg gtggaaagcg atcatcaaga
    17941 ttgttttcca acatacgaaa gcatacgcga tgcatttgtg gaatatgtcc tcagtcttcc
    18001 tcccaatggt gttctaatct tttgtgccga tcagaagggc gctgtagagg ttgctgacat
    18061 tgtcctacaa agacgaagag atatcaaata tgtagagtat ggttttttgg ctcatggaaa
    18121 atatggaatt cgcgattttc agaccaaagc tggagccagt tcatttatcc ttggagagag
    18181 ttcttttcca ataaggattc atttgccagg aaggcacctg gtccaggatt ctgttgcagc
    18241 tattgcactt gtcgaacaga tatttgaaac tgtgaaatcg agaccgtttg aggaatcaga
    18301 gtggatctca gtaggtgaag cattggctgc atttaggggt tcaaaacgac gcagcgagat
    18361 aataggggag tacaaaggaa tactcatcat ggatgattat gggcaccatc caacggcaat
    18421 taaaactaca attgaaggaa taaaaaaatt ctggcctcat agaagactta ttgtggattt
    18481 catgtcccat acctacacca ggacaattgc cctgcaggat gacttcattt cctctctcga
    18541 tggtgcagat tctgttttat tgcacaaaat ctatgcttca gcgcgtgaac ggcctgtctt
    18601 ggggttcaat ggtagaactc tctatcagaa gctttgcgag catcgcccag aactgtttcc
    18661 aatggactta gaacgagata ctaggcaaaa ggaaattgcc tattataaga ataaggctag
    18721 ccaagatcag acaggctttg ttgcctattc cgatgagccg cttaaagcgc tagatgctct
    18781 ttctaatctg ttgcagccag gagatgtttt tttgactctg ggcgctggtg ataattggaa
    18841 actaggtaag gctcttgctg aaaaaataga aaagaatgga taagaagcct taacatggaa
    18901 gcaattgagg aatcaggaga gcagaatcaa tgaagagtat gaccggattt gcccatagtc
    18961 caatcttgga aaaagaattt caaggtactc ttacgctaaa atccttcaat aatcgttttt
    19021 tagacatatc gatttttctt ccatcatatg catctcgaat tgagcccctc attcgagatt
    19081 ttttaggatc acgcatactt cgaggcaagg tcgaatgcac tttcaagcta agaaaaataa
    19141 agttctcact tgaagatatt gagcttcaat ttcaggcagc cactcttctt tctgaacaat
    19201 tgcgcaagct tgccgaagca tgcggtatag aaaaggattt atcgctcgac cttattacaa
    19261 aatttccagg aattgtcgat atgaacacag agattgaaga ggattacctt tggcctgtat
    19321 tgatgccaat ccttgaagaa acctggctga aatttgagga gagtcgaatt cgagaaggta
    19381 cagcgacaga agagaatata agaggccagc ttcagagact gaaatcgaag cttattgaaa
    19441 tcgaactcca agcggattcg ctcgagagaa tggtgtatga ccagttagta gccagattca
    19501 aagagctatt atccgaaagc tatgatgaag ggcgggtact tcaggaagtt gcggtgcaac
    19561 ttgttcgcct tactatcaat gaagaaattg gtcggctcag agcccacatt gatgcttttg
    19621 aattaatggc aaaagaacca gcctgtggca agaaacttga ttttctatgc caggagatga
    19681 atagagaaat caatacgata ggttcaaaga gcatgcttgt ctctgtatct catgcggtgg
    19741 tcgaaatgaa agacgctctc gaaaatatta gagagcagct cagaaatgtg gagtagaata
    19801 ggcatgggta tcattgcgat atcggggaaa tcaggatgtg gtaactcaac cgtgttacgt
    19861 ctggttgctg agaaactcgg ttacgaacca gtgaattaca cttttcgcag catggcagcc
    19921 gacatgggaa taagcttcca agaattgcat aagaaagcgc aagaaaatct tgaatttgat
    19981 cgcaggctag acgaacatca ggttctattg gccagaaagg ggaataccgt gatcggctct
    20041 agactggcaa tttggatggt gcctgaggca gatctaaaag tctatcttaa ggccagccat
    20101 gccgtgcgtg ttaggagaat ccatgccaga gaggatggcg atattgaagc catagatcaa
    20161 tttacaaaag agagagatgc caaggataga ggcagatatc ttgcgctcta caatatagat
    20221 atagacgatg tctctaatgc ccatcttgta atcgacactg agcgatggtc tgcggatcag
    20281 attgccgata tcatagtgga cgcataccat ctgattcctt taggtgaccc atatggaagc
    20341 agcctctgaa aataagcgag ttgcctttgt aactggtgca acgagtggcc ttggtttagc
    20401 gatctcaaga tcactgattg atattggatt tattgtctat ggagcaggac gcagaaaacc
    20461 ccctctcgat ctacctgata attatgtcta tgtgcaaacc gatgtggcac tggatgaatc
    20521 ggttaacaag agcagagatt ttgtgctttc tcagcaaagc cacatcgatc tattagtttg
    20581 cgcagcgggt tttggcataa gcggatcgat tgaggatact cctatagatt cagctataga
    20641 tcagttcaat gtgaattttt tcggcgttgc gagagtggta tgtgcttttt tgcctggtat
    20701 gcgcgagcag ggtaggggtc gaatcatcat tatcggctct atagcaggaa agaccggaat
    20761 gccattccaa gcctactatt ccgcaagcaa atttgctctt gaaggttttg tagaatcgct
    20821 gagatacgag gtgcgcagat tcaacatcga agcctgcatt gtggagccag gcgatttcaa
    20881 aactgggttc actggctcaa gacaaaagat cattgcgccc gaaagtcctt actataatca
    20941 atttgagaga gtaataagtg tacaggaaca tgatgagata catggattgg atccggcaaa
    21001 actgggagaa aggattgccc gattggccgt atcgaagcgt cttcctgcac gccttaccat
    21061 agggccttta tttgaacgtt tggccgtatg gattcgaagg atccttccag atgcttggtt
    21121 cgaggccttt tatagaatgt actatcatct ttagcataga gatctcgaga attcttatct
    21181 tatgcgctat gatagagctc tctctatgga cgcattatat ataggggaaa tatatgcatg
    21241 ttgcgacatc atctatagaa aaaaagcatt ttggcacact ctatagcgga gaggaggtct
    21301 ctcttttcat tctcaaggcc ggatcttttc aagcaagctt tactgattgg ggagcaacat
    21361 gggtcagttt aataatgcct gacaaggaag gccaaaagga cgacatattg cttggttttt
    21421 caactctatc tggttatgtg ggaaagcatc catattttgg ttcaacaatt ggtcgttttg
    21481 caaacaggat aggcggcgct cggtttgtac ttaatggaat tggctacaaa ctctggacaa
    21541 acgacaattg caatcatctg cacgggggtc gcaagggctt tgacaaattt ctatggaatt
    21601 atgaattatc agcatttgat ggagagcctg ctattagatt ttcgagaact agccctgata
    21661 aggaggaagg atatcctggc acactctatg ccgaagttat tgcttctcta tcttctttag
    21721 gagaattgca tttgcattat tatgctgaga cggacgcaat gaccccggtc aatctaacaa
    21781 atcatgcata tttcaatcta gcgggcgagg ggaaagggac aattctagac catgagcttg
    21841 aaatgcaggc aggtatgtat ttaccagtag atgaatacct aattccaaac ggagagattt
    21901 gttctgtaga gggtacggcc cttgatttca gatgcccaaa gcgaattggc agagatatac
    21961 aagaagtagg caatggctat gatttttgct atatcatcga taaggatcgc ttgccactta
    22021 aaacctttgc tattgtaact gagccatctg gtggtcgaag ccttagtata tcgaccacat
    22081 tgccagccgt tcagttttat acaggcaaca acctggcagg aacttttggc aaaagtggct
    22141 ctatctatga caaatatgcc ggtttctgtc ttgaaacaga gatgtatcct gattcaccaa
    22201 acaaacaaaa ttttccttct tgtattctgc ttccgggtat gccctgggaa catgaaacag
    22261 tatataaatt cagcttacaa tgttaattaa ggagttgcat atgctagaag atatttcaaa
    22321 accaatagaa gatataaaag aggacattct tgaaatctgg gggcgtcttt gacgcgcctg
    22381 caatagaaga taagattcat gatttagagg gtctagccag cgctccggat ttctggtctg
    22441 agcagacaca agccaagaaa attctcagcg agataaagat attgagggat cgcttggaaa
    22501 cctggcaaac catgcacaat gatattctca cacttgaaga gatgtacgaa ttagcacgtc
    22561 aggaggatca gcaagatatg gaggccgatc ttcgagtcag tcttgaggag ataagaactc
    22621 gcttcgacag ggcagtggtg atcgaaatgc tctctggaga agcagatcga aacgatgctt
    22681 atctcaccat tcatgctggt tcgggtggta ccgaagcatg tgattgggca agtatgctct
    22741 atcgaatgta tgtccgatgg gctgaaaggc atgattttaa ggtggagact ctcgacttct
    22801 tggaagcaga gggcggaata aagtctgtga caatccaaat atcaggtgaa ttcgtatacg
    22861 gttatctaaa gggagagaca ggggttcatc gtctggtaag gatttcccct ttcgacgcga
    22921 atgccaggcg acacacatcc ttcgcctctg tatcggtaat gccagttctc gatgagtcga
    22981 tagaaatcga tataagacca gaagatatac ggatagatac atatcgagca ggaggagcag
    23041 gcgggcaaaa agtcaacaag acagactccg cggtaagaat tacacatctg gctacgggta
    23101 ttgtagtaac atgtcagaat gagcgcagcc agtataagaa taaagatgta gccatgaaca
    23161 ttctcaagtc aagactgtat gagtattaca aagcagagcg cgacaaagaa accgctaaat
    23221 ttgctggcga aaaaaaggag atcgcctggg gttctcaaat tcgatcttat gtcttccagc
    23281 cttataccat ggtaaaggat catcggaata aattctcggt aggaaatgtt caagccgtaa
    23341 tggatggtga cctggatcca ttcttggaag cctatcttca ttggcaatgg aaagggggta
    23401 catctgttga agaggaagat gaagacatat aaggatcttt tcctatttcc gctatttctt
    23461 tttctcctca tagacactag cctatttgcc cagattacgc catcacagaa taagggcctg
    23521 ggctctattg cacttgtttt tcctatacaa gtctcgaatt tgcctgattt cgatccatta
    23581 ggccaaggaa acctggattc gataattata ttagaattta tacaggatag gcttagtgat
    23641 ccccagtatt atgaaaataa gccttccccc tcggaggcaa acagcctatc aaggctgatg
    23701 gaggaaaccc tgaggttgcg cacaacttct gaagaattaa tagcaataga tatcgaaact
    23761 ctcaagaact ggaattggaa caggactatt caggagaaat cggctctctt tcaggaaggt
    23821 tctcttaaga aatcagatgc agttctgttt atgatgtata cgattgaaga cagtaatatg
    23881 caattagcat cctttctgct aaaaaataca ggggaaagct atgctttttt atcaaacagg
    23941 ttttcggtct ttgatttgtt agatgcagcc agaaagactg ttgactcttt tatagaaaaa
    24001 ggtgcacttc ttgccaagaa gagtacaccc gtccaatctg aattatcgat cgaagaaatc
    24061 gaattgcctt ctattgatcc atggaagaat agagataatt acaagaaagc ccaatcggcc
    24121 tttgagactt ccatgggagg ggctgcaatc agtctgtcga tttcctttgt tgccgtgggc
    24181 ttgtggaata tatatcgcga ggctgcttac agaaatagtg cgtttgatgt tgcagctacg
    24241 atctctgcta tagctgctgg gacgagtgcc gcagtcaccg ccacattttt aactatatca
    24301 atatggaatg caggtaaaat gctcggagca tctcactgaa ttacttgcac agctaggaga
    24361 aataatgaat tttaaggaca aaatcaaagc cctatttcac agaagtcaat tgagcgagga
    24421 agtatttgaa gagttggagg atcttttgat tgagggtgac attggtgcct caattgcttt
    24481 tgaagttgtg gatgcgctta aagacacctg caggaaagat aatatttcaa ccccagaaga
    24541 ggccagaaag ggattaaaaa aaatactgcg cccctttatc agagtcgaag atttcaactt
    24601 agatgataag cgtctcaata ttgttttaat acttggtgtc aatggtgtcg gaaagacaac
    24661 aagttgcgct aagctggcaa aatgggcgga aaacaaagga ataaatcaac ccataatact
    24721 tgccgcctgt gatacatttc gtgcagcggc tattgaacag cttaggattc atggacaaag
    24781 gctaggagct cgcgtaatag ctcaacagca tggatcggat ccaggagcgg ttttgtggga
    24841 tgcaatagat gccgccaaga aaaacaatgc gaatctcgtc attgccgata cagcaggacg
    24901 aatgcatacg agagccgatc ttctacgtga acttgaaaaa ctcgatcgtg tagtaagcca
    24961 gaaagcagga gatgtcaact accgcaggct tttggtgctg gatgctacga cagggcaaaa
    25021 tgccatgcga caggcagaga tattcaatgg ctcggtcaag atagatggcc taatcatggc
    25081 aaaatacgat tccgcttcaa agggaggcat gataattgcc gtaagcaaac aattcggact
    25141 acctaccgta ttccttggaa caggcgagaa gtataatgat ttcgagcctt ttgatatcga
    25201 taaatactta gatgattttg tcggagactg aggtatatag cttcgatgat caagacttcg
    25261 gatgaaaaaa aacagcgccg gatgctttca ggaatgcgtt ttaggctgcc actgtacatt
    25321 gccatccgat ggtcgataac ggggaaaaaa tccgcccaca gctcaggatt gaaatttgct
    25381 tctctgggga ttgctgttgg agttattgca ttaatagtgg tattgagcgt tatgaatggt
    25441 ctccaactgc aatacataga atcacttctt gagacaacga gctttcatat tagagcaaga
    25501 attgaagaaa gcaatctcga tatattcaca gacaccctgc gaaaaaatcg actggtaaaa
    25561 tcggtgactc cctttataga gacgaatctc ctcgtacaaa gctcggagga tcgacagaac
    25621 gtgcttcggg tgatgtggat cgatcctgag gatttagctc tagattccgg ttattgcaaa
    25681 gccttgaata ttcttccttc ggctgcggcc aagagcctgg aggagggaat aatactgggt
    25741 tccgaagcag cacagatctt aggagtctct gaaggatcta tactcactct aagaggagcc
    25801 tttgtagatc ccgaagaggg gattcaaggg tattccgaaa gagtcaaagt ctcaagcatt
    25861 ttccgctcgg ggtactacga gctcgatgct gctttcgcta ttatgcccag actaaaagct
    25921 aaggacctgg caggctttcc tgcctccacc attcttggaa tcaaactgat aaatgcggaa
    25981 aaatctattt cctttgtctc tatgctcgag caggaacctg gaataatcga tatcgaatca
    26041 tggcagaact ataatcgcag tttcttcagc gctcttcata cagagaagat attgatgttc
    26101 ttattggttg gcattatttt tttggttgta gctatcaaca ttcattactc gatgcgtcgt
    26161 agcatagcca ggaaatcgag ggatctggcg atattggcag ccatgggaat gaatcagcat
    26221 tcaatatcga gcatttttat gttagagggg attctggtag gtctggcagg tgctctattt
    26281 ggtattgcta tcggtattcc aattgctcag catgtcgatg gtattataaa cggcgcaatt
    26341 gggttgatcg aatcattcat ctcttttctt tatcgcctcg ggtttgttaa aggagttccg
    26401 gatctcaggg ttttctctcc ctcgatattc tatattgaag gtattccatc tcggatattc
    26461 atctccgata tagctgttat tgcagctttt gcactgattt tccctgtcat cgcagtttat
    26521 ctagcttacc gtcgctttag aaatgtatcc ccgctggagg ttctgagaag tgagtaatcc
    26581 aataatagaa tgcagggatc taaccaaaac cttttcttcg agtgctgaag tattgtgtat
    26641 tctcgatcgg gcgaattttg cgatgaagag aggccaaata tgtacaattc ttggtcctag
    26701 tggatcaggc aagagcactt ttctagctat cctaggcagc cttgagcgct tcgattctgg
    26761 agatgtgcat gtcgatggct atgatctatc ctcgctctcg gaaaaatccc tttccatgtt
    26821 taggcgaaac atagtcggat ttgtttttca atttcacttc ctgctcaatg attttactgc
    26881 gctagaaaat gttgctctgc caagctatgt agcgggcacc ccaaaaaacg aggcatggga
    26941 tagggctcgg agacttttgg atatggttgg acttggcaag cgtctggatc attttccatc
    27001 acagctctcc ggtggagagc ggcaaagagt cgccgttgca agagcattga tcaattcacc
    27061 atcgattgtc cttgcggacg aacccactgg aaatctcgat gcttcaagcg ctaaagagtt
    27121 gaggtctctg cttcaaaatc ttccagaatc ggcagggaca actcttttga tagtcaccca
    27181 tgatcccgat ttggcagcca ttggcggatc atcctacctc ctgaaaggag gtcgattcga
    27241 atgcataaaa taagactgag tgggcttaag ccattctctt atcttggatt tcgtatgctg
    27301 ttgggaagac ctgctgcagc cagagaaaaa ggtgcgcata gcgctcgcca tggtgttttt
    27361 cttggcgcta tcttgggcat tggcattagt attgtgcccc tttatatcgt tatttttgtt
    27421 tctaatggaa tgatcaaagg catcaccgac cgctatctcg aaaccaagac aggtcacata
    27481 cagatgagta ttcccctcga tttgagcgaa gaaaataggg tacagagaat agagacaatt
    27541 gaggaacttc ccggtgttgt atcggcatcg tacgaaatag atggccttgg acttgccgct
    27601 tcgaccaaat gctcgacttc agcgcagatt cggggcttag acgagtctat cacatcagat
    27661 aggggataca gagaatacat tgagatagat gccggggatc tatttcctca aaaagagaac
    27721 gaggtagttc taggacgcta ccttgcaaaa actcttggtg tagagatcgg agaaactgtc
    27781 tcgctgatga ccttgcggga aacagaaggg gaagaaatcc ttccaaaatt gagcattttc
    27841 agagtagctg gaatagtgtc cagtggttat cgcgaacttg atgccaattg gttcataatc
    27901 cattcccgag ttgcccggcg cattttgagt tccgatatgg catattcgta tattggaata
    27961 aaaacaaaag atccttacgg acctgatctt gatgatataa tgaacaatgt caagaaacta
    28021 tatccagaag ctcttgtgcg gccctggcga gatatagaac aaagcctatt taaatctttt
    28081 tctagcacta gatctatgct agtattcata atggctattg caggcattgt agcggcagtc
    28141 aatttatctt ctgtactgac tacttttgtc atggaacata gaaaagagat agcggttttg
    28201 cgttcttttg gtgtatctcg gaagcaaacc atgctcattt ttctcttggg agggacggtt
    28261 acaggcatta taggttgcat atcaggaagc gctgtaggtg ttgcaatatc cctttgtatc
    28321 aaccatctac tctcggcaat tcaaaccttg atcgattttt ttacgaaatt atttagtcct
    28381 tcctctcaag cgataacctt acttaacccg gattattatc ttgaacgaat cccgatatca
    28441 atgaatttga gatatatagt ttttatcttg tgctcaggaa tactagtcag tatgattgtt
    28501 tcccttattc ccgcatttag atcgtcctcc atcgcacccg cagaattgat ccgccatgat
    28561 tgaatatagt gatgagtggc tgcagaattt tcaatcgtgt gatacaattt gtgcgtcata
    28621 gaggatgaag gggtatttgc aatggcgaag agacaaaacc gctcagcggc agaattgatg
    28681 acagaacagg aacctaaatt tggacaaaaa acgggtgcgg taggagaaaa aaaggatcca
    28741 gatatggaaa tccagaagga aagggaagac aaactgagag cattggaagc agccagatta
    28801 cagattgaaa agcaatttgg ccaaggttcc ttgatgaaac ttggagccca tacgattgct
    28861 cagggaatag aggtcatctc atcagggtcg atccttctgg atgaggccct cggtataggc
    28921 ggttatcctc gcggccgaat catcgagatc tacggtcccg agtcatctgg caaaacaacg
    28981 ctagctctgc atgcaattgc cgaagctcag aagaaaggcg ggatagcagc attcatcgat
    29041 gctgagcacg ctctggatcc gatatatgca cgaaatctag gtgtcgatat aaatgaactc
    29101 tatatttctc aaccagacaa tggcgaacaa gcgctggaca tagcagaatc tcttatccgt
    29161 tcaggtgccg tggacataat tgtaatagat tctgtggctg ctctcacacc tcaagccgaa
    29221 attgaaggtg aaatgggcga ttcccacgta gggcttcagg ctagacttat gagtcaagct
    29281 ctgcgaaaac ttgccggtac tctctcaaag agcaagacaa ttctcgtttt tatcaatcaa
    29341 atacgcatga aaataggtgt gatgtttggt aatccagaaa ccaccacagg cggcaatgcc
    29401 ctcaagtttt attcttcagt tcgcatcgaa gttcgaagga tcgatacaat cgaaggagga
    29461 cagaacgaag aagcaatcgg taatagggtg cgcgtcaagg tggtcaagaa caaggttgcg
    29521 ccaccattcc gcaaggctga actcgagatc atattcggta aaggcatctc ttggagtgct
    29581 tctcttctag atgcaggagt aaaatgtaac gtaatcgaaa agagaggcgc ttggtatacc
    29641 tggtctggcg agaagatagg gcagggcaga gaaaatgcaa aggcatatct ggagcaaaat
    29701 gcggaagttg caaaagaaat cgaaacacgg gtccgcgaaa tattattcaa cacagataaa
    29761 tctaaaacaa cggaagccaa tacctcacag acaaatcatt ccggaacaat tgatactttt
    29821 gagacggcca ccattttgga aaattacgaa gacaatgaca ctaaaatcag aacagaggat
    29881 ttgttctgaa gccataatct aaaaggagct ttagcgcttg ctatcatgga aaaagtatgg
    29941 ctaggactag gctcaaataa aggagattct ccttctattt tagattcagc catccaagag
    30001 cttgcaggct ttataataga gcttcggcgc tctggacttt ggcgttcccg cgcccgctat
    30061 tacacagatc agcaagattt tcttaacctt gcaatttgtg gttttaccga ccttacgccg
    30121 cgcgaattac ttatccagat ccatcgtatt gaagcaaatc atggtagaga tagaagtaaa
    30181 gagatcataa aaggccctcg aacccttgat atagatattc ttatatacga tcaaaaaata
    30241 gtggcagaac cagatttgat aattcctcat cctggtctcg cagagaggaa attcgctcta
    30301 ttgcctctcc tagagcttga ttacgaactg attcatccag gtctcggtgt ttctctgcag
    30361 gagttagcag cttgcctccc acctcaaggt atttaccccg tcaatgacaa tcgatatgat
    30421 tggccttact cagaatgact aaagaattag acgatgattc gattcaggaa aaaaattctc
    30481 tcatatctaa tggcagaaca ttctgcctga gcgatttcga gggtcctctc gatcttctgt
    30541 tgtatcttat aaaaaagaat gagatgaaca tcaatgacat ccgtatttcg agcatcactg
    30601 aacaattcct acagtgctta gacagcgatc catccgcgga tcttgacgag atatcggaat
    30661 tctatcaaat ggcagctacg cttctttata tcaaatcgcg tggactcatc cctcatattg
    30721 aagatgaagc tgaagagctc gatgatccaa gaagaaatct tatcgatcaa ttgattgaat
    30781 atcatcgctt caagaaactc tctgagctca tggaacagcg cgaaatggag gtggagtggt
    30841 ttgtcgaaag aaaggtcgca cagcgcacac tcccttttgc agacaatcca gatatcgatc
    30901 cttggatacc tgccgattca tgggatttgc taagaatctt cgcttctatg attcgaaact
    30961 ttagttcaga gcgcatcatc gatctctatg aagaggttag tatcaatgaa aaaattgcgc
    31021 ttatatatga tgatttaatt tctcggaata cttctgccat ggatctcgca tgtgcgtttc
    31081 tcgctgtgct ggattcgaca aaaaataaat tgataagtgt tcgtcagcat aagctctttg
    31141 gcgacatact gataattcca tattcgacga cggagcctca aaatgaacga acagcttgat
    31201 ctcgatgcag cattgattag cgcaatttta tttctagaga atgagcctat ttcagaagaa
    31261 aaacttgcaa aaatctctgg cttgacattg gaacagacat cactggctct tcagaggatt
    31321 tgttcggaat tcgatcagcc gatccatggt tttgccccaa tcaggagaga agagagatgg
    31381 atttttgctc ctaagctggc tttttgggat aagctaaaag atcactatgg caaaagaaac
    31441 gaaatgagac tctcgcgtgc cgccatggaa acccttgcaa ttatcgccta ttctcagcca
    31501 attactcgag cggagataga ggcaattcgt ggggttaatg tcgacgctat ggttaggctt
    31561 cttcttgaaa aaaaccttat agcggaaatt agcaagcgtg atgtgcctgg aaagccgatg
    31621 caatatggta ctaccaagga attcctaaga tgttttggct tgaattcgat cgaggatctt
    31681 ccgaagctcg atgaagtaga aaattctaga ttctcaagac tggagacacc agaaggatga
    31741 gtgagtccat agagaaacca atgcgcctac atgcttacct tgcttctctt ggcatagctt
    31801 ctcgacgttc ttgcgaggaa atgattagag aaggaagggt gaccgttgac ggtgtaaagg
    31861 catccattgg gcaaaagatc atctgcaatc aaagaataga aatagatgga cagccaatca
    31921 ttgcagccta tcccaaaaaa ttccgctaca ttcttctaaa taagccaaaa ggctatctct
    31981 cttcgctcag cgatccatac gggcgccctt gtgctatcga tctaatatca ggcgaagtca
    32041 atgagcgaat ttacaacatt ggcagactag accaatggtc aagtggtctc cttctattta
    32101 caaatgatgg agacctggca tctaagctca tgcatccctc aggccaaatg gaaaaagagt
    32161 ataggatcca gactaatgtg ccgattccgg ataattttgc atcgagcttt atgcgaggaa
    32221 ttattattga tggtgtacga tatcgcgccc gttctgtcca tatcacagga cctaatagcg
    32281 ctctgattgt cctaatagag ggaaaaaatc gcgaaattcg ccgcgtgtta gagcattttg
    32341 agcttcatac aaggctgctg gagcgcattc gatatggtcc catctgcgat ccaggcctag
    32401 caccaggaaa ctggagagag ctcacagtag atgaagtaca agcactcaag actctttgta
    32461 atccgaatca aaaagccaaa gggaagggac aatgagagtg gcaatagatg gcccggcagg
    32521 ttcaggcaaa tccacaattg cgaaaatgat cgcagaaaga cttggttttt tatatataaa
    32581 ttcaggcagc ctttatcgcg caattgcatt tcttgccctt cgtgaaggtg ttgcatggca
    32641 agatgtagaa aaaatgataa acctcgctaa aaagcatcgc tttgattgtc tgtctgatgg
    32701 gtcggtcatt gtagatgaag agtgtcttaa ggaagagctt cggacaccag aggtagatga
    32761 tatcgtctct caagtctctg caataccaga aattcgcgct gaagtgaata acatcattag
    32821 aagaatatcc gaaggtaagg atgttgttag cgagggtagg gacattacaa cagtcgtctt
    32881 tcccgatgcc gatgtaaagc tatatctgga tgcctctcct aaaattcggg cagaaagacg
    32941 atatcgcgaa agacttagca agaaagcttc gaccaaggag gaaggagccg aagccgattc
    33001 gatttcaatt gaagaagtac gcaagaatat cgaaatgcga gataagctcg atgctcaaaa
    33061 acccatcggt gctcttaaaa tagcggaaga tgctatatat ttggatacct ctgacttgac
    33121 catagagcaa gtttatctaa gggcaaggat tgcacatggc agataatatg gaagtgggaa
    33181 tggacgtccg agatccctcc aaggacgata tccaaacaca attgcaggag cagtacctca
    33241 aatcatttga gggcctggag gagggagatt tagtcgacgg aaaggtaatc caaattgctg
    33301 gcgacacggt ttttatagat gttgggtata aatcagaagg aaaactaaat atattggagt
    33361 ttggcgatac cccgctcaaa cccggagatg ttgtcaaggt aattttgatt cgcaaagaga
    33421 cacattcagg cgatataata atttctaaga agaaagccga tgaaaaaatt ttctggaaat
    33481 cgatagccaa tgcattcaaa gagcatcagc ctgtggaagg cacgatcacg aaagaaataa
    33541 agggcggatt cgaagtcgat cttggtcatg gattatcagg ttttctgcct ttaagtaagg
    33601 cagatgttca acgtgtagaa aaaagtgaac agcttattgg aatgcgatgc aggttctatc
    33661 ttgaacgcct gtattcagat aagaagataa acatcattct aaacagacga aaactgatgg
    33721 aggaagagat agagcgcaaa cgtgagtcct tcttccagga aacgcagatt ggcgactctg
    33781 tcagaggtgt ggtaaagagt tttacaagct ttggtgcctt catagacctg ggcggattcg
    33841 acggccttct gcatatcaat gacatgagct ggggtcatgt gactcgcccc aaagattttg
    33901 tacgaaaagg ccaggaaatc gaacttaagg tgattcgtct agatccggaa gaaaagcgca
    33961 taaatctttc gctaaaacat tttactccgg atccatggaa cagctttgaa gataaatatc
    34021 atgtaggtga tatcgtaaag ggaaaagtca ctaagctcac tgattatggt gcctttatcg
    34081 agcttgaaga gggtatagag ggtcttgctc atatctccga attttcatgg ttgaaaaaag
    34141 tgcgaagacc tgaggatgtg ctaaaagctg gtgatgttgt cgactgcatg attcttggat
    34201 atgacatccc ggcaggcaaa gtctcgctag gtctcaaaca ggtacaaccg aatccatggg
    34261 acacaatcga tcaaaggtat cccgtaggta cacggctcaa gagaaaagtg gtaaaggtta
    34321 caaatgccgg agcgtttata gaattagaag aagggatcga tggttttctt catatagatg
    34381 attattcctg gacgcaaaaa gtaaagaatc catcttctgt gctgcatgag ggtgatgaaa
    34441 tagaagtcat tgtacttgag agcagtcctg aggaaagaaa tatccgctta ggtgctaaac
    34501 agcttacgga agacccatgg cgttctttcg ccaagacata taagccaggc tcaattgtag
    34561 agggaacggt atcttctgta actgacttcg gtgtatttgt aaaggtgccg ggcaatatcg
    34621 aaggtttgat taagaagcag gatttggtat ccgacagaaa tgaatcttat gatgaagccc
    34681 ttaagaaata tcatcctgaa atgcccatca aggcagttgt aattgatctc aatccggacc
    34741 gccagaaact ggggctttcc ataagggact tggtccaaaa acaggagcgc gaggagattt
    34801 cccgctttat tcaggaagat gaaggagagg caggctatac gctgggtgac ctcttgaagg
    34861 caaaggataa cgagaacggc tgagcacttt tgttcagtct tctgcaatgg tagaaatggc
    34921 ttctgaaata gattcggata gattccgtac gttgatcgat atcaacgcgc aacttaattc
    34981 cagctacaca aattatcgct ctttgctgaa gactatcgtg gaatcggcag cgagacttgt
    35041 cggagccgaa gcggcaagcc ttgctctgtt cgatgcctcc acagaaagcc tgagattcga
    35101 aatcgcgctt ggtccaagag gtaccgagct ccaggggaag tcggttcccg cagcagaggg
    35161 gattgtgggc tgggtattca aaaatcgccg ctctatcatt gtaaatgatg cagctaatga
    35221 ccctcgtttt tcacccaagg tatcaagaga aatatcctat agcactaatt ctattctcgc
    35281 agttcccctc aagataaagg ataaaacaga aggcgtaata gagctgatca acaaggcctc
    35341 tcatggcatt ttctccagcg aagatcttaa gtgggtagag ctttttgcgg tacaagctgg
    35401 aattgccttt gataatgcaa gacagtatga gaaaacagaa caagagctta tctaccttca
    35461 gcataaggtt cagcaaggag agggatggca tcctcttatt ttttcttctg gggtcatgcg
    35521 tgatcgactt gagctggtaa agcgaattgc tccgagcgac gcctcggttt tgattcttgg
    35581 agagagcgga gttggaaaag aacttatcgc cgagcagttg catcttcttt caaaacgaag
    35641 cgatggacct tttatgcgtg tcaattgcgc agcattgccc gaaagtcttt tagagagtga
    35701 gctttttggc catgtaaaag gagcctttac tgacgctata gaaaatagaa ctggaagatt
    35761 tgaagctgct aatggcggaa caattttcct cgatgagatt gcagaactcc ctctgaagct
    35821 tcaatcaaag cttctccgag tcattcaaca aaagacattt gagagaatag gctcaaatga
    35881 gacaataaaa gtggatgtgc ggataattgc tgcaacgaat agagatatag aaaaactagt
    35941 aaagaatcag gaatttcgaa gcgatcttta ttatcgcttg aatgtactgc ctatacaaat
    36001 accacctctg agagagcgtc tggatgatgt gccaactcta gcggagtatt tccttaaaaa
    36061 gttcacaagg gaaacaaaca agggaatcat gcgtttttct gaatcggcaa tgcaaagcat
    36121 gcttagctac ggttggcccg ggaatattcg ggagctggaa aatgcggtag aacgcgctgt
    36181 ccttctcgca aaaggacagg tgatcacggc cgatgacctg atgataggct ctaagtcgag
    36241 cattgaggat gaacaatttg gcggaaaaga tctaagagag gcaattacca tctttaagaa
    36301 gaattatata gaatccgctc tagagaggaa tcgctggaat cagacagaga ctgctcgaaa
    36361 tctaagaatt cagaggacct acttgtcacg attgatcaaa gagctcaata ttctgcaaaa
    36421 caaggagtaa tttgtgaatc aagaagaaat gcaacaagca cagcacaata aggaagagaa
    36481 gaaaagctct tcggagagat tcgtcgactt tataagcaag aatagaagaa ttataatcgg
    36541 cctcggcatt gcaatcgtac taatcatcgt tgctatagga atttatactg cagtctctgg
    36601 aaatatcgct tctgcatcca gcagagcgat ggaattggcc gaccaaaaag cgctacagtg
    36661 gtcgcaagag accgatgaag aaaaaagagc cgaaattgag agttccctga tagccgaact
    36721 ggactccata gcccaaaaat ggccaaggac acttgcggct cagcgagccc tgcttagaaa
    36781 agccgcgctc ctaagtcaaa aaaaagaata tgcagaagcc gaaaagactg ctcttgaggc
    36841 ttataggaga aataaaaaat cctatgtagc gccgattgct ctcgagctcg ccgctgttgc
    36901 agcagaagaa gcgggtaaca ccgatgctgc cctcgaacac tacacattga ttacaaagga
    36961 ttacaagaag gataatcctg cagcggctca cgcattcttc aatttaggcc gcataaagga
    37021 agccaaaggc gattataagg gcgccatcga agcctataat caactcatag catcatttgg
    37081 agattccgaa tgggcaatgt tggccaaaaa ccgcgtgata tacttgaatg cgcagggact
    37141 agctggttga gtcaattctc aattatggcc tgattttggt aaaggcttgg gtttgatttt
    37201 gtgaatggct tgatttttgg taaagaagga agctagaaga gatgaaaggg gaggggaaag
    37261 agaaaggaat aagattcttg cttcagccca tagccgcact tcttattata aatatatata
    37321 ttataatgac atcgtgtggc cttcctacaa cagattattt gtatactccg aaaggttttt
    37381 cttcagaagg tggagcactg gttcttgaac atgatacaaa gaatatcgat gagctatctt
    37441 ccatatttct aggatatgaa atatattatc gcatttttga tgatgaagat gacgcagtag
    37501 atagccgcaa ttcaatcagc agcatgaaga gctccaatat aattcagaca gccaataatt
    37561 ataaatattt cccattgaag catagaaaaa aatctgaatc tggttggtat tctcttgaaa
    37621 ttagtccatt aattaaggtg tctagcaata cttatacaaa atattacctt aatatgaaat
    37681 cgcaagaaac atggacgctt acggatgaag acttaaaaat tgtgattgat tctattgttc
    37741 gaaacagaag tgatactgcg agtagtgctg aaattgaata tgctgatttt tgtttgtctt
    37801 cagaatataa ggtcaatgat cgagattatg ctggttcatc tcctccagag aaagcagtct
    37861 atatagtgtt ctttgccttc gcttatggct ttgacacttc gactatgaga aatgtctata
    37921 gcgatcctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tggtggataa
    38041 aataatagga caaagaacaa ctgaaaactg atatcagttt gttcgcataa tgtatattct
    38101 gtctaccaat ttgcttcaag gattattcga tgatctatgg gagccaggcc acaactaatt
    38161 agatcatatt ctgaagaagc tcatagctat tcttgttttc gtcataacca tagcagccgg
    38221 ttctcttggc cttgaaacat ttcatagaag aatcagccaa agatgatcca atatcggaat
    38281 cttgatattg caaacaaaat tcataggaat tttctttcag aattttaaaa aggctaagag
    38341 atttaccagc acttcctgga aatttctcta ataattcaat ttcgttgcta gatgagatgc
    38401 tggagctatc gagatttatt ggtataaaga agtcggagta tttctttaat aaatcaactg
    38461 ccattgaaaa agatgaatca tttccaaagc gatatttcca tttggtagcc ttaagcatgg
    38521 agcctattgt ttttttcctc tgttgaagga atttgctagg ctttaacgca ttcatatgaa
    38581 gaccttttgg caaattaata ttgattaatt cgagcaattg cttctgataa tcgcaaataa
    38641 actggcaggc cttttggatg ttttcttctg gtacatttat ccagacagca atgatctcgt
    38701 tttcgccttc aacaccaaga ggtaaaggcg gagacagctc aaattttggt tgaggattgt
    38761 agccttgagt gtaagcagcc gaaaaaccag atactttgaa tgctctaaaa aaagccctag
    38821 ctacatcatg catgggatat aatactgcta tatctttttt cctccagaca cctataagct
    38881 tgattgcgac aatcgatctt actctcgact tgtcatcttc gttatctttg aataggtcat
    38941 cgataatgcc aatcagcctg cttttatcaa tttcttctga gccattgtca atatcttcaa
    39001 tagccttcgc ttttccaatt ataataatcg aatcttcact ctccgcatca ggtttagata
    39061 gtatcatatt agatactact gaattctcat agccacagac accgcaacta tttgtacaat
    39121 tttcattaca aattaaagtt aattgattgt tttctgcttt cttcttttcg ttctttagcc
    39181 atcttgagga gatttttaac gaaatctctt cccatggcag atcttcattt tcggcccttt
    39241 ttgctaagaa tttctcccaa gcaccctccc ctttttttga attatagttc aaaagcgcat
    39301 tattccaaat attgcgatct aaattctctt cccatgcgtc cagtctagca ccttgtttat
    39361 atgcttgcag aataatctca cctatagatt cgtctcctct agaaatcaaa ccttcgagaa
    39421 ccgatagaaa aggtgaatga tagcttattt caatgtttct ggtagaccta agcttttttc
    39481 gcatatattt gattattgta agagactcat tttctgataa ttgtccttct ctttcaaaag
    39541 gggtatgagg ttttggaacg aaagttccaa tattaacatg tattgcaata tgctcaatac
    39601 ttgcaacatt attcagataa gaaatgattt cttctgcttc ttccacgcct ctttcagcta
    39661 ctggaagacc aatcatgaaa tagaattttg ccgatctaaa acctttgagc ttggcttcat
    39721 gaagtatgga cttgactttt tcaaaaggga ctcgcttatt aatgccgcat tgccatgaat
    39781 caagaggcgt ttctattgca aaggtaagac ctgattttct aacttcggat attttttcta
    39841 tcagtggcag ggtgaaacta tccaccttta gacttggaag ctgaaaagat acattatgct
    39901 tggaccagga agaggtaagg tcctggaaga gtcctacaat atcaggatag tcaccagaag
    39961 aaagagaaga aagtgtaatc tcagtatatc ctttttttat aattaaatcc tctatttcct
    40021 tttttattat tgaaggagat ttgcatcgct gggggcggta gaaatatccg gcatggcaaa
    40081 aacgacagcc attggggcag cctctcatta tctcgacaga accatgagag tgaactggat
    40141 ttattactgg gtatggaaag ctgggtacga attcggttct atagaagtct tggtatatgg
    40201 ctcgcctcac ctttttcttc ggttgtaatc ccagttcctg aagaaaagga gatatccata
    40261 ttgaaggatg taaagccaat aaatgcaact tttcaattcg agtcgctccc ctgctcttta
    40321 actcagctag cttaagcatg agctctgtaa atccttcttc agcttcacca atccatactg
    40381 catccaaaaa tcgtgcaaaa ggcattggat ttgtaattgc cggtcctcca gcaattataa
    40441 tgggatctcc ctcgcctctc tctatattct ccagaggaat ccaagctcta tcaataattg
    40501 caagaatatt agtggcaagt agctcgtatc caatactaaa tcccaataca tctgtttcct
    40561 taagtgggat tccgctttca agcgtgtaaa gaggaatgtg tttatctata agcaaatgtt
    40621 cgaaatcccg agcaggagca aagactcttt cacaaacgag agcatccctt cgttcattga
    40681 gcatcgcata gataattcta agagcgttgt tcgacattcc aatctcatat agatctggaa
    40741 aagaaagggc aattctcagt cgtgaatcca aagggtctat tggtggaaaa gcatcgcact
    40801 ctcctccagt atagcgagct ggtttttgta ctttaagaag cgcttctccg atatctctca
    40861 gaggatcgat aaactcaata ttcatgcgga atacctgcgc gcagaaattc cggttaggat
    40921 gccaatagaa atcaagcctg tccagagaga cgaaccacca taagacacga aatacaaggg
    40981 tattccagtt actggcatta ttcccattgc cattccaata ttgatcataa aatgaaaggc
    41041 aaatatgcct aacagccctc cgatgacgaa ttgactaaag cgatccttgc atgtttctat
    41101 gagaaaaaca catcgaaaga atagaattcc atatagaata aagagcacgg tacaaccaat
    41161 aaatccgaat tcttcagcta ttatagaaaa aatgaaatct gtgctctgct gtgggatata
    41221 tcttgcatga ctatgagtgc cctgtaaaaa ccctttccct aaaaagcctc cagatccaat
    41281 agcagtgatg ctctgaagaa tgttccatcc tgagcctttt ggatcgatac taggatcgat
    41341 aaatacgagt aacctcatta tctgatatgg ttttaagatg ctatgagctg caacggctcc
    41401 aatcatcgaa atggccaaaa tggccatact atatgagaac cagtaatagt atcgcttctt
    41461 gaacttaagc catccgattc cggaaatgat cgaaatcaca agcacaaaga taagtatagc
    41521 aatcagtaca ttttcatgag tgatcgcagt gtagatgatg ttttcagggt tggggtgatt
    41581 agatgcatac aatggaagac tcagaaatag aaaaactcca aatatcgata aaactccaaa
    41641 tagaatatat cgaaaatcaa catccgccaa tgtgagcatt acaagagcag caggaacaaa
    41701 tactagtgcg gaaccgaaat caggttgcgc cagaattaag gccaatggga taatcataat
    41761 aatgccggtt atcaagagct ttttaaagct tgaatcaaat tccgcggatt ccaggtagcg
    41821 tgcgagcatc aaaactgttg ctacttttgt gaattcggct ggttgaatgc cgatgccgcc
    41881 aattccaagc catgatctgg ctccattgac cactcttcct gccagcagcg taattataag
    41941 cagtaatatt gcaaaagcaa agaaaaccca tacaatatcc ttatatctgg aaaaatcgaa
    42001 aagcgctgct gcaattagta agaagattcc gataccaaac cataagattt gctttaggta
    42061 ttcgttagaa ataatggctc cttctgcatc gatccccgat gaatatatgg ctattattcc
    42121 cagtgcgcag agcaaaaagc atgagaaaag tagataataa tcaatatctc caaacagtct
    42181 tttaaaattc tggaatttca ttctcttctc cctatcaagg aagaaccctc cagacgcacc
    42241 ccgacagctt tcgccgcgtc atgggcattt tgattagcat atattgcttg atagattaca
    42301 ttagcagcat aaggagccca ccattcccaa ggattcgagg cttctatcat ggtggtcacg
    42361 actatctgat ctttagccgg cgcatcgtag ggtccaaaag atacaaacca tgaatgccat
    42421 ctatccttga gacccacttc agctgttcca gtttttccgg caacctgaac cacttttgtg
    42481 tttattggca ctctagccgt gccatggaca atgacatcgc gcagataaga tttcagagtt
    42541 tcaaatgtct tggctgaaat tgaagactga tgaaggattt ctggctgtat ttcatcgata
    42601 agagcgcttg tagtcggatc tctaacttct tttagaatat gaggcttata aatgattcca
    42661 tcattgacga tcatggccat catatttgcg agctgaagag gagacgctaa cagatatcct
    42721 tgtcctatag agaggttcat ggtatcgcca tgtgtccagg tatcgttata cttttcttct
    42781 ttccatgtcg gtgttggtaa aagaccagca ttctccgcag gcaaatcgat gccggttgcc
    42841 tttccatagc caaaatcttg cgcataactg acaatattct ctattcctag ataatcccga
    42901 ccagcaatcc aaaaatagat atcacatgat tgtgcaaggg catctttaag atccagagct
    42961 ccatgcccag gcttcttaat atggcagctc catgaacgac caccatatat cagctcaccg
    43021 ctacaataga tttttttatc tggagaaatt gaaccttctt cgagaatacc ggctgttaag
    43081 actgttttga atgtcgaagc cacaggatag gatgactgat aggctcgatt gagcataggt
    43141 ttttttggat cattcagaag atctatgtat gcctttccga tattgtccga catgaaaact
    43201 ctggaatcat aataaggata ggtgaccatt gctaaaattt cacctgtcga tggcttaagt
    43261 acaattatgg atccctcgcg agagccaaga gcttcctctg caattctttg aatattgccg
    43321 tctatgctca gaatcaatcg cttaccggga atcggttctt gtatttctgc agcattgctc
    43381 gaaatatctt ttccataaac atcgacggct ttagatatcc agccatcctt acctttgaga
    43441 agttcttcat gctctaattc tatgcctgct tttccggtaa gatcttctaa tatatagcct
    43501 ttattatata gcagtttata ttcatctcga gtgattgctc ctacataacc aatgacatta
    43561 gaaagcgatc tcagatcagc ataattccta aggggtttag aactccaaga aacgcctgga
    43621 aattcatcaa ttctggctgc aatctgtgtc acggcttcca ggcttgcatt tttggctacc
    43681 accactcttc catatgaata taagctcaaa tcaggaaact tgctctctat atcctctgta
    43741 ggaatgccca aaagcaggct gagtttctta ataacatcgg attttttcga atcaggtaat
    43801 tcggaaggcg taatatatac cgaataggct tctctattac ctgcgagaag atctcgtcct
    43861 gatctatcat atatttcgcc cctgggggca ggaattcttg ttgattggat ggagatacgc
    43921 tgtgcttcgc ttacaaataa ttcttttctc aaaatctgca tagagaatag atttattgaa
    43981 tagacagata gaagcgatat tagtagagcc ttcaaaatgc gcatgcgaag tgtgattttt
    44041 tttggaagca tagccattat atctgccctc ttgcgatgat aatgctcacg gcatagcttt
    44101 cttgggatta tgctcgaaca actgcctaat aaaagaaagt agaaagaaca gcagtattgt
    44161 aaataatccg ttcaacatca gctcaatacc gaattccata ctgaaaatat gataataatg
    44221 aatgccagcc gaaaacagaa gatagattac tttagataat aacgccatta taatcgtcgc
    44281 tcccgctcca agaaggaaag gaacaataaa aatgtccttc tctgtaacat agcttataag
    44341 cgttgcaaag tagccacagc ttgtatatag aaaggcataa tatccgagcg gagatgcgct
    44401 gagaatatcc gccgcaatcc ccaccaagaa tgaagcaatg ataccctgcc cttctctgtt
    44461 gataaaagag caaaagagaa gcacaccaag agccagatca ggaataatgc ccatgataat
    44521 cccgcgcgac aaccatgttg attgtatgaa aagcataagt gtcgcaacaa tgcctgatat
    44581 aaagatactt ttcattgaat tgcctctttt ttaggcgagg aaagaggttc aatatctatt
    44641 gcgaacacat actctatcct gctgaggtca attgccggtt gcacacttat cactaatgag
    44701 ctggagagtt cattgagtcg ccaatcctcc actcgtccta taatgatctc tgatgggaag
    44761 atctcatcca agccgctggt aacaacgata tctcctcgtt tgaggagatc cgcctcggcc
    44821 ttggggacat aaagcagatt aagtgggaca tcccgaaatc cctggccatt tagcattcct
    44881 tcggcacgcg tttttgcaag tctggcagct gcgaagaatc gctgatcata gagaggttgg
    44941 agtatacttg tcgaggcctt tacctcgatt atcttaccaa ccaaaccctc aacaccattc
    45001 tgataagcca caactggcat atatttcctt ataccattta tatatccctt atctatcacc
    45061 agtgaggaat acatattggt aggatctcta gctatgacat gagcagcgat tttctttgca
    45121 tctggcaatg tgctaagccc taagagctct ttgaggcgtt gattttcttc tctcagtgct
    45181 gtgtaatctc gtacctttag ctcataatcc tggactttct ccagtagtgc ttcatagtct
    45241 ctctcaatgt ttcgaagctc agatactgca cctatggttc cagtaaagaa ggagccaatt
    45301 gcagataagc ccttttgaat tcctccaatt atataaccat tgaaaaaacc aggcactttg
    45361 actattgtct ttgttgatat aaatagaaca ataattgaca gcaccagaca aattccagct
    45421 ttcttgtgca tcgctcgagc aggtccatgg atttggctgc cagtcttcat gctcaacctg
    45481 ccagtatcag agatttagac tctcgtaaac actgctgctt ccgatattct tctgcataat
    45541 ctcaaaatac tttccagcgc caatggcaac gcattcaaga gggtgctctg aaagaattac
    45601 gggaacaccg gtttcctttg ctatcaattt tggcagtcct ttaagaagag ctcctcctcc
    45661 cgaaagcaca ataccgcgtt ccacaatgtc ggatgccagt tcgggtgggg ttttccctag
    45721 cgttttcttt atttcctcaa ctataagatt tactgaatcc attagcgctt cgcgtatttc
    45781 tacggaatcg acttcgaaac ggcgaggaag cccagtgatt gcatctgtgc cctttatttc
    45841 gattttttct attgttcttt ctggatttgc atttccaatt tctattttga gcctctcggc
    45901 ggtctgctca cctattatca ggttatgtat atttcgaatg tgttttgcga tagcttcgtc
    45961 gaattcatcg ccaccaactc gaattgcatt tgtcactacc atgcctttta atgatataac
    46021 cgaaatttcg gtagtgcccc caccgatgtc acatatcatg tgcccagcag gttctgttat
    46081 gggaatgcct gctccaattg ccgcagcaag cgattctgct attaccttca ccattctggc
    46141 accggcctta taagtggagt ctataaccgc attttcttcg acctttgtaa tgcatgttgg
    46201 aacgccaact actatccttg gtttgaaaaa aaggttcttg ggaaataccc tctgaataaa
    46261 atatttgatc attttttcag tcatatctgg atcggcaatt acaccatccc gcagcggacg
    46321 ctttgctata atatcagtcg gggttctcga aagcatgctc ttggcttcgg taccaactgc
    46381 tacaatacgg tttgtccccc tctccaccgc cactacagat ggctcgtcga gcacaatgcc
    46441 tttgccattc atatagatca gtgtattgca tgtcccgaga tcgatgccaa tatcagggct
    46501 gaacatgtca aggataccca cttcctttct ccttttccta taatttttcg gccaatcgct
    46561 ttcttgcatc cgcatgattg gggtcgattt gaacaacctt tctgaaagct gcccgagcac
    46621 caagctggtc gttcaacttt gcatatatga ggccgagacc ataccatgcg tccacattga
    46681 ccggatcttc ttttagtact ccctgatatc tatcctgtgc ggattgaata tcgccggaat
    46741 cgaagttgtc ctgagccagc aagagcgtag ctttgagtct gatttttgca tcggatgccg
    46801 agctctccag gcgcaaaagc aagtctcttg ccttctcaat ctggtctgta ttcatgtaac
    46861 ttatggcagc tgacaataaa agagcgtcag aagtgttatt ctcaagagct agttcatacc
    46921 aggaaacagc tcgactatag tcttttctgc cagcatatac gattgctaag tactgtggaa
    46981 tgtctttctc tttgcttccg ctttcttttg caagcaagaa ataacgttca gcaagatcga
    47041 accatggttc acctttctga taataagctt ttgccagtat ataatagact tgtcccttca
    47101 tccttgaggg aagtccaacg gcgagggctt ttctgagagc aataaccgct tcttccaact
    47161 cttgttgtcg aatttcctct tcctgagcgt tgagagcgga ataataatat gctataccgc
    47221 aaatactaag ataatattcg tcgaaaggat agctttctat agctttctgt gcttcggaca
    47281 gtgtctgttc tatattattt ccggaattcc agactttagc caaagcggat cgggatatgc
    47341 tattcttagc acttctgcct ctgatggcaa aatagaaacc aaggaaaaca cctacagcaa
    47401 tcaatgctat cgctatgaaa agccaagggt tcttcttttt tgtgtgatga gcttttcctc
    47461 taaagaatct atccagcatt aaaaataata tatcaaaaaa aggcgggcct gtaagccggg
    47521 ttctgtatgt ctgagcgatt ttcgcgctct caacatggtt accatctatc tcgatgcatc
    47581 attactgatg cattccagcg gtctacccgc gattttgtcg cttatggcga cttggacggg
    47641 tcatccatcg ctgtttgacc ttgctcgcga tgaggtttgc catgccattc ccgttaccgg
    47701 aaatgcggtg agctcttacc tcaccttttc acccttgctg cgtattagcg cagcggtata
    47761 ttctctgtgg cactttctgt acaggagaga gcttgcgttc cgagcattcg gaactgaaaa
    47821 cccttcctgc cccggatgtt atccggcatc gaacccttcc gagcccggac tttcctcagg
    47881 aattacctgc ggcaacccag cccgcctaaa acaaaatcat tcttccatat tatcgataag
    47941 aaaatcattg tcaataagcg gctcagtctg attctttctt tttttggaag agcttttttt
    48001 agacgaatcc gaggtgtctt cggattcgtc ttccgactca gcatcatctg catcttcgct
    48061 atcttcgtct cccaagtcca caagagaacc agtgagtatt tcttcgatcg atccttcaat
    48121 ctccgtctct tcataatata ggattcgcga acaatatggg caaaaaacta tcttatcagc
    48181 ttgacggacc tcgtttacaa aatttgcggg aagaatcatg tggcaaccag tgcatacaac
    48241 accatggatc ggcacaatgc ccatgcctga tttacttcga atgatgcgct cgaacttgaa
    48301 catcaaatca tcttcaagtt cctgagtcag gctattctcc ttggttcgaa gattctcaat
    48361 ctcttcgata atctctttac tattctggtc tatctctgct cgtttttgct ctatttcgga
    48421 ttccagagcg gaaacagaga tctctatacg atgcaaccta tcatcgatct cggcaaaacg
    48481 gcgttcttct tcttggattc gatgcctaag atcctgttct ttttctgaag cttcacggat
    48541 ttccttgttt acagcatcat attctcgctg agtctggatg ccgtccatct gcctttcagc
    48601 attttcgcgc ctcatttctg cttcggcgag tgcctgacgg agctgcttaa gatttgcttc
    48661 tgtcgtagta aactgttctt ttgcatccac caaggatttt ttttctctgg aaagcatttg
    48721 attctggatc tcaatagtct tgggcatggc ttccagctgt ttttctagtt ctacacgatt
    48781 gctgagcagc tcttggtact ccctgagagt ctcgaataaa tcatccattc tcatgggcat
    48841 gggtcacctc tatcaatgat ctatatagtc acga
//
LOCUS       DAOOWY010000003        47302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_815_length_47302_cov_10.167058, whole genome shotgun sequence.
ACCESSION   DAOOWY010000003 DAOOWY010000000
VERSION     DAOOWY010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 47302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47302
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_815_length_47302_cov_10.167058"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1038)
                     /locus_tag="PK105_00460"
     CDS             complement(100..1038)
                     /locus_tag="PK105_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOU60203.1"
                     /translation="MADDLHALAESDFYKARSKAISASIFGMLRPSMSDLMPFDQAKE
                     LLKPRAETYRGVTAVPIDRIVGSEGRYRDFNRFFFPKKEHLKARWTTIDELQYKDVSL
                     PPVVLYEMGGVFFVRDGNHRVSVARAKKQEFIDAEVISLHSEIQLDPEMTIEDIKKAV
                     IEYEKKQFYRETNYPNIVGADDLNFSEPGRFDSIKEHVLVHKYFLNQHLPEEIPFHIA
                     LYSWHDNVYQPISQSIEAENILSLFPGRTVSDLYLFLVAHWDELKRKSGGYVAIDEAA
                     ESFKNEIKRNRNSSFEHACRIFGSISKKVENFIAKL"
     gene            complement(1028..1696)
                     /locus_tag="PK105_00465"
     CDS             complement(1028..1696)
                     /locus_tag="PK105_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU60204.1"
                     /translation="MIRPVRILCVADEVDLLVYSVQICERFSDVDLILSAGDLPDGYL
                     EYIASMLNKPLISVAGNHDKSKQDIENAGIHYPRNQTANFGQISFRIEKESGISILGL
                     PGSIRYNKGQNQYTDAWMTFRIILMLPRLLLRRLLWGRSVDIILAHSPPRGIHDGGDP
                     AHKGFSAYRWLIRLAKPYYFVHGHVHVYDMQALREIDYYDTSVVNVYGHRVITLPRED
                     KNGR"
     gene            complement(1693..3459)
                     /gene="mutL"
                     /locus_tag="PK105_00470"
     CDS             complement(1693..3459)
                     /gene="mutL"
                     /locus_tag="PK105_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOU60205.1"
                     /translation="MGNIRILPPETSRRIAAGEVIDRPSSALREIIDNSIDADAHNIS
                     VSIAQGGIEEITVADDGWGMSKEDLLLSISEHATSKIYGPDDILKAKTLGFRGEALAS
                     IAAVARLEIVSKLRGSNEGWRLSSSPLKEPVIEPFACKEGTRLTMRGLFESYPARRQF
                     LKRPQSEAFLCRSTFIERAVSHPDICFRWAVSSDMDVMIASSLKDRILRCYTELSRFT
                     LFEAMAETSELQMHCIYADVANYRRDRKFLQVFVNKRRVPEWNLLSLMEYEFGKYLPG
                     GAHAIAFLFIEIDPSLADFNIHPAKKEVRLKKLAELRTVLHELLSHELVHRYGGMTQA
                     FETAIYPEFKEFVFSTKEKQIAPGLADQPQVLHSTKYDREMGREFTNCSSNSYFVRRD
                     STQLPDDFWHRIKDDNLSTLRYIGKGPGPFLIFELSDALFILDQHAAHERILYNKIKS
                     TRGESQLLLVPYLLEPYENEHYLEESKDNLEFVGYGITRDKNTWIVEAVPAIAASEAL
                     EALVEWLSQPCFETSPIDALIASLACKAAIKDGDRMDEISALKLIQESLELPEPRCPH
                     GRPIYLAFPRQQLYSMFGRTIG"
     gene            complement(3551..4912)
                     /locus_tag="PK105_00475"
     CDS             complement(3551..4912)
                     /locus_tag="PK105_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60206.1"
                     /translation="MKKATLLFALVLFSFPIFAQQGMNPSFGIIANLGTDLLPDPGDP
                     SKFESWSKIGMQPEFSIGKFGIGLDLLFRFKLGTGSNTSLEIYEPDWIPQQGQNIFDV
                     YLPKILYIRYGQQWEDPFYVKMGSISDFSLGNGLIVDNYSNMRFLPQRRVFGMQLGID
                     GSLFGFPYVGLEVLTGNVSKFDVIGGRIYVRPLAFMEESLFGRLQLAATGVFDRDPLL
                     YTGTPYLAQQTRLIYVVGADITLPLIQSPAFSLTPFGEGAYEMNNAIGAIAGIKGRAF
                     DILSYLAQFRYLQEGFIPSYFDANYDIYRADRFVYIETNPAKNNFIPGWLASLGLDLF
                     KSALSFGVILDAPFKELPSPATDNPADYAHIKGSLNLARGLIPNVNIAARYEKFFLGR
                     KSNDVLKDLIDFEDSSIGMTISYKAGTAIVSMDYAYIWNPTKNSGAGAFDVVSSMSLG
                     FEL"
     gene            5008..6456
                     /gene="pyrF"
                     /locus_tag="PK105_00480"
     CDS             5008..6456
                     /gene="pyrF"
                     /locus_tag="PK105_00480"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOU60207.1"
                     /translation="MGFFEYIEERADKMDTFLCIGLDPAFSNSEIEKRGSLECAACAL
                     EQNLRIIEATASYALAFKPNIAFYEAMGNAGMSALYKTLAQIPEEIPIIIDAKRGDIA
                     NTAEAYARAIFGELKADAVTLNPYLGLDTLEPFLRWSDRGIFVLCRTSNPGAGFLQDI
                     TVNKSPLYSMVAQKCSDLSVDVGLVVAGNDLEALQKVRKVAPSAWFLSPGIGAQGGRA
                     DLAFAAGARSDGKGILVAAARSISEAQRPDDAALELRDVLRRARDAFLFKAAVKTAKP
                     SKKAEDEVASLKREFVDVLLSTECFRLGEFVLKSGKKSPFYIDLRNLISDPEAMRIAG
                     RAYAHLASSCDFDIIGGIPSAGLPLATVASLELGKPMIWPRMPAKDHGTGKRIEGRYA
                     KGDRVLLLDDLITTGASKIEAKDILKSEGLEVRDLVVLIERGSEGRVDMEREGVRLHA
                     FMHVNELFEALKENGRISEAQYSDLVDFGKTI"
     gene            complement(6569..7150)
                     /locus_tag="PK105_00485"
     CDS             complement(6569..7150)
                     /locus_tag="PK105_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOU60208.1"
                     /translation="MRIFLISGSRCSGKTSFCNSLIKSAEDKGIVCRGCIEISQRSEN
                     GIPHSIDLYNVSNGDTFLAASRPIDRGDVPFEYSPDAFAHIRKSYIQTVESDIISQHP
                     QNIPDKKREICLIDEIGPLELEHRGGHLSLLEFVLGTKSPDALVLTVRTGLKYSLMSF
                     LINHGISADHIYDLELEKLTISSACIYVLSFLE"
     gene            complement(7174..7818)
                     /locus_tag="PK105_00490"
     CDS             complement(7174..7818)
                     /locus_tag="PK105_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOU60209.1"
                     /translation="MSQKTKLNYVPSIRRFPSYLHIIRAFQRTGEPYISGTLIANELN
                     LEPIQVRKDLSITGIVGKPKKGYPVDQLIAAIEHYLAWDVEQKAMIVGAGNLGSALTG
                     YQEFRNHGLHIVAAFDSDPLKVGKNIHQIHVYQMNQLREIADEMKITLAILTVPSPYA
                     QSSCDAIVDAGIKAIWNFTNVKLRVPDDVIVQREDLSSGYAILSVMMRTKARLS"
     gene            complement(8138..9034)
                     /locus_tag="PK105_00495"
     CDS             complement(8138..9034)
                     /locus_tag="PK105_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOU60210.1"
                     /translation="MPCFTKFMQILEWADWSAPPEQMNCSLSFQCIIQTIKELIPAIE
                     SAQLSDEKLDSYRIQELLDKAIRLYLLTPALVNVLLNYKICVEHDLPLHPTVYYELKE
                     ARKYRIRHSLAEIQQANEQYWQSIEVARLCYQCAPQAISAIDELCFGIPSGIASFLYT
                     AVQDHYTWLGSQPSLLLELAEKISREFRPSLIVAAAHGSIMPALILSELLEIPVYFIR
                     FSMFKRHDEEPIISLSDQAWLFDYRNKNVLLYDEDVARGNTLDLFSRRLSPLFGEVRT
                     ACSIRHAGSCVHADFSGRVWWD"
     gene            9046..9738
                     /locus_tag="PK105_00500"
     CDS             9046..9738
                     /locus_tag="PK105_00500"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOU60211.1"
                     /translation="MADTKAEQEVLDMIAEQRLPFSAAILNGGHARRFGGIDKQEVEI
                     EGVPIGFLLAYRLGKIAQELLIVGKPHKLYEDLGASQFEDDIKEGGPVCGLFSALVNA
                     SLEWVCLCAADMPFVSLALIRYLYVQSMSKEVDIVLCKTDDTLQPFFAFYKRNLVSEL
                     YSYIQSSSDLSFRHFIKMEKHLIIQENDIASICDPGIVFQNINDRKSLERARKVANRQ
                     VLGLDEFHIMEE"
     gene            9740..11395
                     /gene="ftcD"
                     /locus_tag="PK105_00505"
     CDS             9740..11395
                     /gene="ftcD"
                     /locus_tag="PK105_00505"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOU60212.1"
                     /translation="MEKPLVECVPNFSEGRDRAKIEAIADAIRSVQGVFLLDVDPGVD
                     TNRTVYTFIGPPEAVKEAALEAARVAFKLIDMRTHRGAHPRIGALDVCPFVPVSGVSM
                     EECIEISRDFGKKLSEELNVPVYLYEKSATRPERRSLADIRAGEYEGLENKLADPAWQ
                     PDFGPTHFTPRWGATVTGAREFLIAYNINLNTKDKRVAHDIALSIRESGRTIRDSNGN
                     TIKVPGLLQAVRAIGWYIESYQCAQVSINILDFRTTPLYVVFDAVKKEAEARGIYVIG
                     SELIGLIPLEAICVCGRYYRQKAGKSPGLSDEELVELAVQTLGLRSVQPFEPEKKIIE
                     WAIRKEGALVSKKVSSFVDTVSSDSPAPGGGSVAALAGALGSALAAMDANLTVGKKGY
                     EGVSETLSTLGIKAQRLKNDLLEIVDEDTAAFNAVMDAMRLPRSTDAERIARDLAIEK
                     ANKLAAGVPLKTARFCLEAMRLCLEAVETGNLNSATDAATGCLMAWAGLSGAVLNVRI
                     NIKTLSDEQFACTASKECDSLEIEATQLRQDLEKSIPSILGLR"
     gene            11406..12614
                     /locus_tag="PK105_00510"
     CDS             11406..12614
                     /locus_tag="PK105_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU60213.1"
                     /translation="MSYIFSRRSNAGEIEPNELSRVRAQRHGFGLPIKDLSNTNPTKV
                     GLDWKDLDLISLATKDNVTYDPEPKGLYRAREAISDHFALYKRNMHPDNYFLCASTSE
                     AYSWLFKLLCDPGDAVLVPKPGYPLFQHLAELDSVQVLAYRLEYAHPSGWYVNLDELS
                     SVLAADREKRIKAIVIINPNNPTGSYIRKTERTTIIGLCEKYGLALIADEVFFDFPID
                     DNIERKTFVGEDRVLTFVLDGLSKRMGLPQLKLAWITLSGPNQEVSRAKKRLDLIADT
                     FLSVSTPIMNALPFLFEHERAFLCKSLSRIRENYASYREILTGGTSPHRVLCCQGGWT
                     ALIESPSFFEEDRIAQRLLEEKGIFAQPGFFFDIDNGVHFAFSLIIPSISAFRWCTEY
                     RHFFEALENS"
     gene            12656..13540
                     /gene="folD"
                     /locus_tag="PK105_00515"
     CDS             12656..13540
                     /gene="folD"
                     /locus_tag="PK105_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOU60214.1"
                     /translation="MSAVLIDGKKIAEEIRNELVQKVNFLKGKGVVPGLAVILVGDNP
                     ASISYVTAKEKACAEIGMKSFETRMAENSSEQALLDRIMECNRDPNIDGILVQLPLPR
                     HIDERRVIEAIDPNKDVDGFTPVNLGRMLLEQPSFIPCTPFGIIELLKRSGVPTNGAR
                     AVVVGRSNIVGKPLANLLLRKSVNATVTICHTGTKNLGSHVREADIVVACAGAPGLVS
                     VDMIKPGACIIDVGVNRVPDSTKKKGYRLCGDVDFEGASRIAGWITPVPGGVGPMTIT
                     MLLSNTIDAAARISNIAI"
     gene            13622..14458
                     /gene="folE2"
                     /locus_tag="PK105_00520"
     CDS             13622..14458
                     /gene="folE2"
                     /locus_tag="PK105_00520"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOU60215.1"
                     /translation="MVDVQSFRDDRNVAIQKVGVKGLRYPITLLDKAEKYQHTTAAVN
                     LFADLPHDFKGTHMSRFIEVFEEYRTDLSMPNVIKMLRKLRAELDAQTTYTDIHFPYF
                     IKKKAPVSEQASIMNYECFYEASCSGKGHRFIAGVIVPVQTVCPCSKAISDGGAHNQR
                     GIVTLQVSLGPFFWFEDLIRIIEESASSELFTLLKREDEKYITEKAYMQPRFVEDVVR
                     EVYLKVDALQRFPWFSVEAENFESIHNHSAYAYVEKSDRSKIEPVSGEHGRLDRLESY
                     YP"
     gene            14528..14884
                     /locus_tag="PK105_00525"
     CDS             14528..14884
                     /locus_tag="PK105_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60216.1"
                     /translation="MESNYPVDPKTLNDKGTKGVMSSVAGLGFLGVEALLGIPVVGTV
                     ISAGLIGLGAIGLLGKTKTDKISGTVLGVAGIAGLTTLFLPGVAHSLLSLGGIGLLGY
                     GLFNLAGFIRGLKKKK"
     gene            14881..16248
                     /gene="miaB"
                     /locus_tag="PK105_00530"
     CDS             14881..16248
                     /gene="miaB"
                     /locus_tag="PK105_00530"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOU60217.1"
                     /translation="MNNYHIETYGCEMNKAESAAMEATLREHGWKQSEEREAQLILLN
                     TCTVRATAENRVWNRIHQLTARKKEHDFVLVVIGCMAEQYKNAIKKKAPGVDYIIGTY
                     QRQSFSLMLDSIANGQKIEVLEENPIYIFSEDHHETGAFRSFIPIIHGCNNFCTYCIV
                     PYVRGREISRSPLDILREIDRLENLGVREIILLGQNVNSYTWEKDGVSLDFPGLIKMI
                     AAHLRKKQESEGTIESYNASPKKSKRGIGWIRFITSHPKDLSKELMAVMAEDPFFCHH
                     LHLPLQSGSNTILSAMNRKYTREYYLSVIDALRNAIPDLTLSTDIMVGFPGETEEDFE
                     QTLDLMRSVRFSYAFMYHFNERNGTFAAGMPNKITEKIKKERLARVIALQKEITSSLM
                     RGRIGHVDEVLIEDRSKRSTREILARTSRDEMVVFPGSATRIGQFAQVRFISVVGNTL
                     RGEEV"
     gene            16260..17294
                     /locus_tag="PK105_00535"
     CDS             16260..17294
                     /locus_tag="PK105_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU60218.1"
                     /translation="MKRNRIGITRDRSSLNFAASFVLFLSLFILIINSSIEAETYKSL
                     SQARQAASKTKSQDALRDVLLSAVSQLSVRDGIALCQEYEAKTDQNLKAELRGILGGL
                     YLLLGQTKEAASWYIKAASIDAKYTNEALRLSIAIGDQKNALALLKNESISPEFKYIF
                     DIWLSLYDGEYASASSKARDALAIVTKPQLRRELLLLQYFADFGEFGAGISSLVKDFP
                     NSIESDLIIGRVFPSSSLMLLLGFSWIKGPAPLVDYGVTSNNSQIAEEHSAASGIQWL
                     QAGYFSSKDNAERLSRNLTNQKFKTRVIELKNQAGEPRWAVHVAAEEDWKKTQSKLKD
                     LGYESYPAGN"
     gene            complement(17291..18721)
                     /locus_tag="PK105_00540"
     CDS             complement(17291..18721)
                     /locus_tag="PK105_00540"
                     /inference="COORDINATES: protein motif:HMM:NF037483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60219.1"
                     /translation="MNNFKSLVSHLYFVAFFAAFLIAVYLIVIQRQETLQASVPIENR
                     TIVLESLSDKSQVSPQNNSLQHAKISPLPGDIFLKTIDIDVDDDEDFEQVILCKKTAG
                     STVLWMIIADFSPNLGAYLRHFEAEIRASKLESITVQPLDVTGDGLSDLLIQGLDSSN
                     NQTLTIFRRLLNRGYSLIFSETGLSINLQDSDESAIPSIVVESLPNKTGLVSYKTYIW
                     NRNSLFLEKVSESPLRESNSISEGPSGSDAQSYLSWLSRFWKKNSDASNICSLYLDTN
                     SNALILEGQQIQQRWIIKSAERKGNRLYLTCRLSELSYLDRIFIIDAKGQNVISVSII
                     DQQVSHFRRDEGWSGKYESYDYMKEAKDNKTILSSPDHSQFLGRYLGDDHSVLVLDQS
                     KNILVLNGKYREGAARIYYYDGYQVLDFLQIQSNGLVGDRLLFIVSIDQSENGKINRL
                     ILRPAEIGADGVRMEYRTPYIFIKTS"
     gene            complement(18742..19941)
                     /gene="rocD"
                     /locus_tag="PK105_00545"
     CDS             complement(18742..19941)
                     /gene="rocD"
                     /locus_tag="PK105_00545"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOU60220.1"
                     /translation="MNSQDYIALDYQYGAHNYHPIPVVISMAKGCKVWDPEGREYYDF
                     LSAYSALNQGHLHPHIVNAVKSQIERVTLTSRAFHNDKMGPFLKKLCEYTGYEMALPM
                     NSGVEAVETAIKAARRWGVEKRGIENGRQSIICANGNFHGRTLSAVSMSSDPDSYINY
                     GPYVPGFIKIPFGDAKAVSDAIDENTVAVLVEPIQGEAGVVVPPDGYLSDLRRICTEA
                     GILFILDEIQTGFCRTGRHFAWNYENARPDIMCLGKALGGGIIPISAIVADHEVMDVF
                     TPGTHGSTFGGNPLACAVGIAALEVLEQENLDERAFRLGERFREVIKKAKSPHFRLVR
                     GKGLLNAVVFEEGFDAYPTCIDLKESGILAKQTHGNIIRFAPPLVITEDELEEALEKI
                     IYVLDRA"
     gene            complement(20140..21258)
                     /locus_tag="PK105_00550"
     CDS             complement(20140..21258)
                     /locus_tag="PK105_00550"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOU60221.1"
                     /translation="MKSIAVLYGGRSGEHEVSLISASSILAYLDKDKYSIVPIGITKR
                     GEWFLQHPPEWAYSRPENGQMRSLPPIERSERVLAVPGEGLWLETTTKGLQKLNIDVV
                     FPVLHGTFGEDGTIQGLLETVDLPYVGADVLGSAVGMDKEVSKRLWLSAGLPVVDYIS
                     VRKEDVSDENLKILARKVETRFGWPCFVKPACSGSSVGTSKVTHSEELKDAILSALRW
                     SEHALIEAFINAREIECAVLGNERPMVFPPGEIVPSHEFYDYEAKYKDPQGATLLIPA
                     PLLEETKNRIMSLALSAYKYASVKGMARVDFFVEKITGEVFLNEINTIPGFTAISMYP
                     RMCIDAGVSYSELLDKLLELAIEQYAIKRELEYDFSKA"
     gene            complement(21262..22950)
                     /locus_tag="PK105_00555"
     CDS             complement(21262..22950)
                     /locus_tag="PK105_00555"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOU60222.1"
                     /translation="MQKRLSDLIQGLDICSIEGDIHHLISGLFYDSRECKPGSLFFAL
                     PGLHTDGKKYIIQAIQNGAVAVIYEGLLEKNLREFMLKDPSARALNPALIRVTDCRWT
                     MSAISASFFENPSDSLCVIGVTGTEGKSTTVSFIYQLLNAAGFKAGFFSTVMSDIGEG
                     EQPNPQHQTTPEATTVQQMLAEIRNHGLQFAVIEASSHGLSPRTARLAHVHFDIGLVT
                     NVTHEHLEFHGSCEQYRSDKANLFRRLGEVEPKKLNKVSHAINEHNSNIIISPTGIIC
                     ADDPNASYFVHESAVPCLTYSSKGALADLSAHNIIEDANGVSFEIEGLLEMPIGHNFR
                     TEQEISARRFENVMPSTLSARINLPGTFNVQNALGAILAASAATGLHWHNFVPLLPGL
                     RPIKGRMQRILEGQPFEVIIDYAHTPSSFKEILPSLRKERKGRIICVFGSAGERDTEK
                     RPQQGRIAADYCDILILADEDPRGEDPMAILEEIAMGCPELSREDRLYLIPDRPEAIR
                     RAFNLAKPGDLVLLLGKGHENSIIYSDRTIPYDEERQARSILADLGFEITNKGK"
     assembly_gap    23020..23119
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23250..23633
                     /gene="queD"
                     /locus_tag="PK105_00560"
     CDS             23250..23633
                     /gene="queD"
                     /locus_tag="PK105_00560"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOU60223.1"
                     /translation="MYHIRVETEFSAAHRLFHYNGKCERFHGHNYRVRVWASGKELGE
                     GGMLIDFGILKSALKKLIDEEFDHQDLNQIAELGEDPSAERIATLIYGRLHRDFPSLP
                     ISAVDVFETSSSMARFVPDAWPDAR"
     gene            complement(23635..26187)
                     /locus_tag="PK105_00565"
     CDS             complement(23635..26187)
                     /locus_tag="PK105_00565"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60224.1"
                     /translation="MSASTISEPKIVTEAVAADIANPRFEDAQYRILIVRLSPFRDVE
                     GSYTHQVLFDEARHALPKAYIDFAFMPSFSHRKALSIAKRPWFFGRASNRSATEFNML
                     LISCAFSLELLNIPWLLSQSGIPFSRHERLERYDLPFLFLGGSSAVCSGSLVKISDNK
                     VINSLVDAIFFGEGEGRISEIVRIASDDSKTGLSKLSILAHIASEVEGFWPCDSSFTC
                     NRALSFQRPAVLTSPIVLNSENADTVKLAISAGCIGHCTFCLEGWDRRPYSEKPSDYL
                     SKSAVVLKRASGASDVELFSYNFNMHKDIIQLIQILGRYFMKVSMMSQRIDILSNKPE
                     ILAAELAAGKRSFALGIEGISERIRKYYQKGISEGQIWNSITRIIDNKAREIKLFFII
                     SGFENGHDLEEFENFCDKLAHYKIETQSVTRVIVSAGYLVRLPFTPLQYAPLQGDRAL
                     LQSIASALRISCNKSNLEFRLASSFEDYWMDQLLSLGGSRMHDWLQACPENGFLYDLH
                     VSDRALNSLCAFFNRESCFTQLLQEKPHTYRPDFDFLESARHWQMLAELYDRSIDNLC
                     SPDSKKFYTKMPAIPLISLEAERTIDIIKAQQKAKAHFPSILVRIRENDALAFSTPAY
                     ERAWLLRTLSFLVPNAERALFYCKLQLPNSDWESAIPTKSASMPCKLRIGISGVKYFA
                     IYGPDKTDMERIINLAISKIKHSNETKKIGLSSISSNYSGSTAFLEEIEIIQELPQAE
                     TCRLSFCIPANRDGFLNEVLEGWLNELNLHFTLQKDEDSIIYQTYSAHKANKSVQYIQ
                     YKTSYTNISLCLSIGKKADIALLADFLYDKKCTLEETIFRIEGWDLSNVCLA"
     gene            complement(26184..27494)
                     /locus_tag="PK105_00570"
     CDS             complement(26184..27494)
                     /locus_tag="PK105_00570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU60225.1"
                     /translation="MSLGINTSLIQALEILGINEPTEVQKQAVPLLLEKKDLSMQSET
                     GTGKTLAYLLPIYNTILSNIDEADRRWPVAVIICPTQELAVQVANHSAQLAMALQSKN
                     NGAMSQSDSCQQQGLSKLRNRAPKIDSNLLNSPQVRSLVLLGGTQFTRQKQALKIHPH
                     IIVGTPGRLADLTRLRYFDLSHLQFLILDEADRLFSREYIEPVEFLLSNAPKNCTHVM
                     ASATIPERTRNRAKPWIPKPIVIELEREGVLSQAIEHWAFYSDHRKKIDLLKRIIVAI
                     HPKRCLIFASEAYRVQRAAERLNSSGLQCASLVSRMEKQSRHSIIEQFKTGGISLLIT
                     TDLAARGLDIPDITHVISLDLPEDGGVYVHRAGRTARAGKKGISILIADKLELERASR
                     TATRFGFVFRTKWLEYGTVMEPPVEAFFERVEDMETQKRSKSHL"
     gene            27699..29372
                     /locus_tag="PK105_00575"
     CDS             27699..29372
                     /locus_tag="PK105_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOU60226.1"
                     /translation="MHVLISNSGSIELDALTRSEFNFSVDHLMEIAAIRLWQTLLSEV
                     IVKKNMSSLISAGSSSSRQPGIAAVCGKGDNAGDALAMLRHARLEGYSRLTAYIPEPN
                     TMKNEVISNLRRAERCGTEIICYETIDLKSIGERLSTEDIILDALFGTGIEGPARGRA
                     SDLIQILRDVRIARNERCKKNPLIVSVDVPSGLSDGWRPEFPIVCADITLCIEPMKES
                     LYMPKARPYAGDIIPVGSIFPLWTKASTQKTWLLEEGDMKGFLPPISPWAYKMQRGRV
                     AVFAGSASGAGAALHCVRGAASAGAGYIALFCDEELFAAYLAAIGESAIVRVFSEDSF
                     SSECWDVVIAGPGWGIDPGRERILEMLLQAHIPLVLDADGARLFARMARANQDSSFFG
                     GPIILTPHPGEFSEFIPMLGSDFSGKTSETVASVSALAKKYEIALALRASTTHVGLPD
                     GTCFIYDGSTPGLGIAGSGDVLSGMIGGFLARWIAFSKERKENRESTEPATSSDNPVA
                     RALLGAILVHGLAGKQLWLKKGWFTPLDLANACARISSGAMETDMDNDR"
     gene            29388..29825
                     /locus_tag="PK105_00580"
     CDS             29388..29825
                     /locus_tag="PK105_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU60227.1"
                     /translation="MVGQDDTAGKIESLRSVAGIAIKSDLLFVARRKKDHTEMSHRWE
                     FPGGKVEEGESDESALKREFLEEFGANIDVIRFLGESIFENKGRTRTLAAWEVSFSPG
                     SIRQLNEHVEVAWLPIESISNMELADSDRSLIPILEAAVKPGS"
     gene            complement(29832..30113)
                     /gene="xseB"
                     /locus_tag="PK105_00585"
     CDS             complement(29832..30113)
                     /gene="xseB"
                     /locus_tag="PK105_00585"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOU60228.1"
                     /translation="MKDFEKRIAHLEELAEKMRDPDLPLEKSFGFFEEGVMLARELRK
                     ELDELQGKVEILLNSLEVRENDEAEVPNITKFDFQSSESQNDSQDKQAP"
     gene            complement(30110..31363)
                     /gene="xseA"
                     /locus_tag="PK105_00590"
     CDS             complement(30110..31363)
                     /gene="xseA"
                     /locus_tag="PK105_00590"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOU60229.1"
                     /translation="MNFDSNLDAESRLFSPLSVSQITSLIRNNLENKFPDVIIEGEVS
                     NCKMAASGHLYFSLKDEEAILQVVMFRRDLLTLDFVPKDGMQVWARGAISVYAQRGQY
                     QLIARNVRQTGFGDILAMLEQRRRKFAEEGLFDESRKKEIPAFPTRLGVVTSPTGAAI
                     RDIIRVIQRRNPKVSILILPTLVQGQEAAASIAERIRQANLWNLADVLIVGRGGGSIE
                     DLLPFSEEVVIHAIVESNIPIISAVGHETDWALSDYAADLRAPTPSAAAEMACADLYS
                     IYKEIAHFENIMGQSMKHSIANAKQRLSSISPHIMEDILLRKNLVASQRFDSAIESIR
                     NSFQKKAEDLNHRLVLASSAIEIANPREIMKRGFSVVKKNGVDIVHSSRMLALGDSIN
                     IMFYEGEADADVKATRDYGDKRSSG"
     gene            31448..33406
                     /locus_tag="PK105_00595"
                     /pseudo
     CDS             31448..33406
                     /locus_tag="PK105_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690073.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA];
                     GO_process: GO:0008380 - RNA splicing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
     gene            complement(33526..34269)
                     /gene="trmB"
                     /locus_tag="PK105_00600"
     CDS             complement(33526..34269)
                     /gene="trmB"
                     /locus_tag="PK105_00600"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOU60230.1"
                     /translation="MSKPEMEEGQDRKPEQTQSMSQEIGLLDGSIRSFVKRSGRITEA
                     QRRAIKLLGPKYIIPFSEELLILEELFPQNRPIILEIGFGSGQATWRIAQERPEFNYL
                     GIEVYASGVGRLIMDLELHAIENVRILQQDAIAVLRHMIAPRSLAGIHVFYPDPWPKK
                     RHHKRRLMQYPIVFLMAQKLMPGGYLYCVTDIEEYASFAKASLDACSLLENQYEDFAP
                     FQPWRPETRFENLAKEAGNQHFELFYRKN"
     gene            complement(34256..35587)
                     /gene="hisS"
                     /locus_tag="PK105_00605"
     CDS             complement(34256..35587)
                     /gene="hisS"
                     /locus_tag="PK105_00605"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOU60231.1"
                     /translation="MTVIKPRVLKGFRDFLPDAEITRSSLVRELEQVFALFGFVPIDT
                     PVLEYADILLGKGGGETDKQVYRFLDHGDRDVAMRFDLTVPFARFMAEHVDELYLPFR
                     RYHIAKVWRGENTQRGRYREFMQCDFDIVGTDSGSADADILLTAAKAMLRLDVGEFSI
                     RINHRALFNRFLEHIGASARSTEILRTVDKLEKIGFENAFEKLSKLLGLQLADDILSF
                     IKKEESFEATLEKMIGFCDKNDFQAQSARDRLLEIMEYVAAAGFENRIMLDPSITRGL
                     DYYTGMVVETSLGALPEIGSVCSGGRYDELASLYTERRMPGVGASIGLDRLIAALETI
                     GKIDKSIISTQILIMNQEKGNVGKVATSELHALATRLRDAGFSCEVFPEEKKTLAQYT
                     YAERKHIPLALFTNSAKGPYNIRILAERRNIECLDFDSLCKFIRNFLNVQT"
     gene            35823..36104
                     /gene="rpsF"
                     /locus_tag="PK105_00610"
     CDS             35823..36104
                     /gene="rpsF"
                     /locus_tag="PK105_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOU60232.1"
                     /translation="MRQYELVTVLSSEEEEFKAGKQAIADMLSQYKAIDVKEEDMGDK
                     PLAYPIKKKTRAHYMLYRMSFDPASIVSLERAIKLNPLVLKHLVVKVEE"
     gene            36138..36593
                     /gene="ssb"
                     /locus_tag="PK105_00615"
     CDS             36138..36593
                     /gene="ssb"
                     /locus_tag="PK105_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU60233.1"
                     /translation="MSDINVVVLVGRLTRDSELKYTPSGLPICRFSIAVNRSKKQDDQ
                     WIDEPHYFDIEFYGKSAEGLSKYLFKGRQVAVQGELRQDRWEKDGQQRSKVVIVAGTI
                     RPLGAPSQNSGEMGGRRYAADNIPSKIDSANNQMPIPPTIDEFTDDIPF"
     gene            36615..36923
                     /gene="rpsR"
                     /locus_tag="PK105_00620"
     CDS             36615..36923
                     /gene="rpsR"
                     /locus_tag="PK105_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOU60234.1"
                     /translation="MRDIDESRASNVEAEQQELHRDQDDRNGVRKGKGFFRKKVCRFC
                     TQNVKIDYKDAELLRRYTTERGKILPRRITGTCSKHQRMLAVNIKRARALGLLPYVIK
                     "
     gene            36964..37938
                     /locus_tag="PK105_00625"
     CDS             36964..37938
                     /locus_tag="PK105_00625"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOU60235.1"
                     /translation="MEVSQSIKSESSSKYSWRVFIPYGLLSGFLYASGFLAFAFLIPI
                     QYVFRKENKREGLLSILVSLSIVAAISTIELSRMGWKELPIFLQTLLLPALLLATIGA
                     MNLLKSDAWVKIIAATIVVSVLFAYLFSISIGTPEARRSIADMIAQLLNSSGIQDLDA
                     SAIEQNYVIPAISIIMRSFGVASWLLLAGSWHIGNSLASARIWKQKGEAKKNAFVFSV
                     PSWLLWPSILGWLALICVLYGNERGVFAIIAWNVSLAAACWYALQGFSLISYFFRLRN
                     MQRLAGLLLILLMILMFYEAKIRIAAAILAPMLGVSEVWLQYRMHKGA"
     gene            37941..38534
                     /gene="rplI"
                     /locus_tag="PK105_00630"
     CDS             37941..38534
                     /gene="rplI"
                     /locus_tag="PK105_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOU60236.1"
                     /translation="MKVILNQDIPNLGEIGDVKEVAPGYARNYLLPKKMVMVYNEKTE
                     ALFLKRQAEIQTIKEQKRLSSRTLKERIEAEPLVISMPAGKNGKLYGAVTSHTIAEEL
                     LKKNMEVDRKRIEVPDRSIKSVGNYKIVIKLYEKDEAILHCNIEAQASASAEEKKPSE
                     EAGKKRRTRRQRTESEPATPEEQAAAFEAAVNRGQLS"
     gene            38585..39928
                     /gene="dnaB"
                     /locus_tag="PK105_00635"
     CDS             38585..39928
                     /gene="dnaB"
                     /locus_tag="PK105_00635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOU60237.1"
                     /translation="MAGPSLKDTLPLYNGDAEQACLGALLLDPESINNVIRYLRPDSF
                     YDSANQEVFEALLAMHEKGQKPDLITLSEELRSSGNLERIGGSAYIASLASFTPSAAN
                     IEYYAKIVQEMATRRRLVHLSAETAAMAHEETKDIDAILDEIQAKIFEISQNRRTAEY
                     HSAKEIVTETMLLIEKLSTDPAAFTGIPSGLSDLDALTNGLQKSEFIVIGARPSVGKT
                     ALALTMAAHASINLKIPVAFFSLEMSESAIMLRLIASEARIPADRIRTGRIKTTDYDS
                     LMDAASRIYEAPLYIVDLPNMKLLELRTMARRLVLERGVRAIFVDYLTLITHENADLP
                     RWEQIAAISRSLKALARELDIPIVALSQLKREAEGKQPTLADLRESGSIEQDADLIIF
                     LHRDRETSKTYDSQTDQIETDIIVAKQRNGPTGKAPVWFKSSYAKFVNMERRDRT"
     gene            complement(39947..41299)
                     /locus_tag="PK105_00640"
     CDS             complement(39947..41299)
                     /locus_tag="PK105_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOU60238.1"
                     /translation="MISIILGLIGLSIVVIFHEFGHFVFARLSGVEVEAFSVGWGPSI
                     ISHKGKKTVWKLCAFPIGGYCKLKGEEGFQAAVDQNLQYIPSEKGSFFSATPLKRIFI
                     ASAGPLFSVLFAAFIFILVASIGTTIVTAPNRIILSSEIGSTLQSGMPNPADIAGLKT
                     GDKIISIDGKTIRDYSDLQEIIASNPGKTMSIEVLRNDKKHSLVITPLLDPNSGIGVI
                     GVYAWIDPIIESVQEKSPAAIADLRPGDIIMEADGRPISNTVDLMQVFNNANGSVSLR
                     VKRGDQTLSMIIVAKSLEEAGIRFEGILRTEKAKSLPEAISMGVEETFSTISLEIKSI
                     GMLFRGVNVFKAISGPARLTYLIGTAASEQIKAEGLAGLVPILDFLAFISIALAIMNL
                     LPIPALDGGRVLIYLIELIRRRPINAKSLYRFQFIGVASILLLFLVATMSDIFFFLGK
                     "
     gene            complement(41296..42423)
                     /gene="dxr"
                     /locus_tag="PK105_00645"
     CDS             complement(41296..42423)
                     /gene="dxr"
                     /locus_tag="PK105_00645"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOU60239.1"
                     /translation="MVLGASGSIGRQALDVIQRSADSCNKLELVGFSIHSNRILAKKL
                     KRQFPQAKGAWTGNPSLAPEEMDWNGVDAISNLLLETEAEIVLNGIAGAAGLSASILA
                     LQNKRDLALANKESIVMGYRLLRNLAQKNQCQIIPVDSEHAGIFQIVQRFGRDLVSEL
                     IITASGGPFYALPLELLDAVTADEACKHPVWQMGRKISIDSATMANKGLELIEASRLF
                     DMPQERIKVLIHPQSYVHAMVRTIEGVLYAQISKPDMRLPIQMALYWPEIVETSFGFA
                     DLADISLDFYEPKQERYPMLWIARQAIDEGDAQCIVFNAANEIAVECFDNGRIRFTDI
                     SNLVAKVLDRDWNLPIESFEDIFDIDIKARRIAAHLSESCG"
     gene            complement(42460..43338)
                     /locus_tag="PK105_00650"
     CDS             complement(42460..43338)
                     /locus_tag="PK105_00650"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOU60240.1"
                     /translation="MNENMRARLLLFFVGFPLLILSLFFLQFANYIVISGILLIVQFL
                     SSKELRNMLTRASLPDTGDLATFIGLIPSVLTFISCILYGSLLETAFVLFISWSFCLM
                     LLLIPLVFQKKNSFPDLIKQAASIALVHCYTALLPGFLIFIVSGFPNSQNTIISFALL
                     TFGNDSLAWFFGKYLARKRNLLDVSPNKSISGFIGGTIGSIVAAFIGLGPIAGAWKPS
                     GIAYILLSLSLGLGMAFFVIVGDLFESALKRSAGIKDSGSIVPGRGGILDSFDSLYFS
                     APFFIAFSYTFHLFNL"
     gene            complement(43335..44039)
                     /gene="uppS"
                     /locus_tag="PK105_00655"
     CDS             complement(43335..44039)
                     /gene="uppS"
                     /locus_tag="PK105_00655"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOU60241.1"
                     /translation="MKENRMPSHVAIIMDGNGRWARQRGLARTEGHKEGLKTAKRIVL
                     ASRKEKIEFLSLFVFSTENWKRTEQEVGFLMSLIRAHITKELNFYRKNEIRIVHSGER
                     NGLPHDVLSEIDKAIENTSQYSSLTVNLAINYGGRDEIVRAMQRILASQENDDENPID
                     ELKIMRYLDHPEIPDPDLIIRTGGEIRLSNFLLWQSAYSELYFSPKYWPDFTPEDLHA
                     ALIDYVHRERRFGDAR"
     gene            complement(44052..44606)
                     /gene="frr"
                     /locus_tag="PK105_00660"
     CDS             complement(44052..44606)
                     /gene="frr"
                     /locus_tag="PK105_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOU60242.1"
                     /translation="MESNKAAHEEKMKKTIAAFQDELNSIRTSRASPVLLDKIRVDYY
                     GQKTPLSQVATISVPEARLIVIQPWDRSLFSEIEKAILKSDLGLNPSNDGKVLRIAIP
                     PLTEQRRKELVKTARSIAEQARVAIRNIRRDALEELKKQQGAGGVAEDVLKKEEAELQ
                     KMTDDYISQIARILEIKEREIMEI"
     gene            complement(44694..45545)
                     /gene="tsf"
                     /locus_tag="PK105_00665"
     CDS             complement(44694..45545)
                     /gene="tsf"
                     /locus_tag="PK105_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOU60243.1"
                     /translation="MEIKANEVKALREKTGAGMMDCKKALIEANGDFSAAEKLLREWG
                     IAGIEKRAGRATNEGRIFIAQNDKELELIELACETDFVARNEDFVAAGTRIAESALSQ
                     KASSTNESLEAIVKEIASLIKENIALRRVVHFYAEENEILHPYLHGEGRIGVVVKVRA
                     NDPEAFKHEKIAAFIHDIALHVAAFNPMFLDESKVSAEWITEQKEIFQKQLELDEKLK
                     SKPEKVLEGILAGKLKKLLADVCLLNQGFVRDEKSSVASILAQISKETGFQLEIVDYY
                     FAKVGQD"
     gene            complement(45567..46448)
                     /gene="rpsB"
                     /locus_tag="PK105_00670"
     CDS             complement(45567..46448)
                     /gene="rpsB"
                     /locus_tag="PK105_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOU60244.1"
                     /translation="MAVVTMKNLLESGVHFGHQVKRWDPRMKKYIFAERNGIHIIDLQ
                     KTIQAIKEAYDAVQKVASSGKSVLFVGTKKQAQAAIQKEAERCEQFYVNNRWLGGMLT
                     NFSTIRKSVQRLKKIERMEVDGTFSSLTKKEIASLLKEKTKLEKNLGGIKEMTSLPGI
                     LFVVDTRKEAIAVAEAQRLGIPIVAIVDTNCNPEGITYPIPGNDDAIRSISLFTQIIA
                     NAVIEAGASEGLKIIETLPSEEEQELIVPQKLEEDEVEIDVENYAETPSQSNKEADEQ
                     QEVEQPIIDEDRLYDED"
     gene            complement(46579..>47302)
                     /locus_tag="PK105_00675"
     CDS             complement(46579..>47302)
                     /locus_tag="PK105_00675"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOU60245.1"
                     /translation="LSKHPSLIRLLFVMYGEPILLASESSRRASILESIRIPFIKVLP
                     SIDETIFDNLDPAERVVALAATKAKHGKALWSEKNRSKKVVISSSAKKPLDKNALDQK
                     SNAEPRYVLGADTLVAFKISGRWITLGKPANELEAFEMLSMEAGKRQTVFSGLCLLDM
                     VSDRCYPALSISEVQFAPMTPEEVSWYLSFGEWRGAAGAYRIQEIGSCFIEEIRGSSS
                     GVMGLPIHELYGILHASGYLDS"
BASE COUNT        13088 a        10216 c        10642 g        13256 t
ORIGIN      
        1 gataaggagg gatatccgac ggctaataca atggaggctg catgaagaag ctacatcaac
       61 ttgctcttca tttattataa tacgaaatca ttccaaaagt tacaattttg caataaaatt
      121 ctctactttt tttgaaatcg atccaaagat tctgcaagca tgctcgaaag agctatttcg
      181 atttcgtttt atctcgttct taaagctctc tgcagcttcg tctatagcaa catagccgcc
      241 cgatttgcgt ttgagttcat cccagtgagc aacaagaaaa aggtataaat cagatacagt
      301 ccttccagga aatagcgata gaatattctc tgcttcaata gattgagata ttggctggta
      361 gacattatca tgccaagaat atagagcaat atgaaaaggg atttcttcag gtagatgttg
      421 attcaagaag tatttgtgta caaggacgtg ttcttttata ctatcgaatc taccaggttc
      481 gctaaagtta agatcatccg ccccgacaat atttggataa ttggtctccc tgtagaattg
      541 ttttttttca tattcgataa cagctttctt tatatcttcg attgtcattt ctggatcgag
      601 ttgaatctcc gaatgaagcg atattacctc ggcatctatg aactcctgtt tcttagctct
      661 agcgaccgat actctatgat tgccatcgcg gacaaagaag actccaccca tttcatacaa
      721 taccacagga ggaaggctca catccttata ttgcaattca tcgatagttg tccaacgagc
      781 ttttagatgc tctttctttg ggaaaaagaa acgattgaag tctctatagc gtccctcgct
      841 tccaacgatt ctatcgatag ggacagcagt aacccctcta tatgtctcag ctctgggctt
      901 gagcaactcc ttagcttgat cgaagggcat taaatcggac atcgaaggcc ttagcattcc
      961 gaatattgat gcgctaatag ccttacttct cgccttatag aaatctgatt cggcaagagc
     1021 gtggagatca tcggccattt ttatcctccc ttggcagagt tatgactcga tgcccgtaca
     1081 cattgacaac agaggtatca taataatcga tttcgcggag cgcttgcatg tcgtatacgt
     1141 gcacatgtcc atgaacgaaa taataaggct tggcaagtcg aataagccat cgataagcag
     1201 agaatccctt atgggcggga tctccaccat cgtgaatacc gcgcggagga gaatgtgcaa
     1261 gaatgatgtc tactgatctt ccccatagca ggcgtctcaa caatagcctt gggagcatca
     1321 atattatacg aaatgtcatc catgcatcgg tatattgatt ctgacctttg ttgtaacgaa
     1381 tcgaacctgg caggccaaga atggatatac cactttcttt ctctattcta aagctgattt
     1441 gaccaaaatt cgctgtctga ttccttgggt aatgaatgcc agcattctca atatcctgtt
     1501 tagatttatc atgatttcct gctacagaga ttagcggctt gttcagcatg cttgctatat
     1561 attcgagata gccatcgggc agatctccag ccgacaatat cagatcgaca tccgaaaagc
     1621 gttcgcagat ctgcacacta tagacaagta gatccacttc gtccgcgaca cataatattc
     1681 ggaccggtct tatcatccga tagttctccc aaacattgaa tagagctgct gtcttggaaa
     1741 tgcaagatat attggacgcc catgaggaca gcgaggctct ggcaattcca gggattcttg
     1801 aataagcttt agggctgaga tttcatccat tctgtctccg tcttttatcg cggccttgca
     1861 tgccaggctt gcaataagag catctatggg ggaggtttca aagcacggct gagaaagcca
     1921 ttcaacaagc gcttcgagag cctccgaagc cgcaatggct ggaacagctt caacaatcca
     1981 ggtattcttg tctcttgtta ttccataacc aacaaattcc agattatctt ttgattcttc
     2041 caagtaatgc tcattctcat aaggttccag cagataagga acaaggagca actgactctc
     2101 tcctctagtt gatttgattt tgttgtatag aattcgctca tgggctgcat gctgatctag
     2161 tataaagagc gcatcggata gctcaaagat tagaaatggt cctggccctt ttcctatgta
     2221 tcgaagggtg gatagattat cgtcttttat tctgtgccag aaatcatctg gaagctgtgt
     2281 ggagtctcga cggacaaagt atgaattaga tgaacaattt gtgaattctc ttcccatttc
     2341 cctgtcatat ttcgttgaat gcaaaacctg cggttgatcc gctaagcccg gcgcgatttg
     2401 tttttcttta gttgaaaaaa caaactcttt gaattcgggg taaattgccg tttcaaaagc
     2461 ctgagtcatg cctccatatc tatgaactaa ttcgtgagat aagagttcat gaagtacggt
     2521 ccttaattcc gccagctttt ttagccggac ctccttctta gctggatgta tgttaaaatc
     2581 cgcaagcgaa ggatctattt cgataaaaag aaaggcaata gcatgcgccc ccccggggag
     2641 atatttgcca aactcatatt ccatcaggct gagcaggttc cattccggga ctcttctttt
     2701 attcacgaac acctgaagga attttcggtc tctcctataa ttggccacat cagcgtagat
     2761 acaatgcatt tgaagctccg atgtctcagc cattgcttca aacagtgtaa aacgagaaag
     2821 ttcggtgtag cagcgaagaa tgcgatcctt caaagaagac gcaatcataa catccatatc
     2881 gctcgacact gcccagcgaa agcaaatgtc aggatgagaa accgcgcgct cgatgaaggt
     2941 agaacgacac agaaaggctt cgctctgagg tctttttaga aactgacgtc ttgctggata
     3001 ggattcgaag agccccctca tggtcaatcg agtcccctcc ttgcaggcaa acggctcgat
     3061 tacaggttcc tttaatggag agctggataa tcgccagcct tcattagatc cccttagctt
     3121 gcttactatt tccaatcgag ctaccgcagc tatggatgcg agcgcttctc ctctgaatcc
     3181 aagagttttc gctttcaaga tatcgtccgg tccgtagatt ttggaagttg cgtgttcgga
     3241 tatgcttagc aagagatctt ctttcgacat tccccagcca tcatcggcaa cggttatctc
     3301 ctctatgcct ccttgagcta tagagaccga aatattatgc gcatctgcat ctattgaatt
     3361 gtcgataatc tctcgtagtg ctgaagatgg ccgatctatc acttctcctg cagctatgcg
     3421 gcgagaggtt tcgggtggta ggattcttat atttcccatt tttcgtatga taatggtttt
     3481 ctgtaaaaaa acaaaggcgg cgtccttcta agaccaccgc ccttacactg gagacattgg
     3541 cctcaaaagc tcacaattca aatcctagag acatgctaga aaccacatca aaagcacccg
     3601 ccccgctatt ctttgttgga ttccagatat aagcataatc catagacacg atagccgtgc
     3661 ctgctttata gcttatggtc ataccaatag aagaatcctc aaaatcgata aggtctttaa
     3721 ggacatcatt gctctttctg ccaaggaaga atttctcata tctagcagcg atgttaacat
     3781 tgggtataag gccacgggcc agatttaggc taccctttat atgtgcataa tcagcagggt
     3841 tgtccgttgc aggcgagggg agttctttga atggagcatc caatataacg ccaaagctca
     3901 atgcggattt aaagagatca agaccaagag atgctagcca gccaggaata aaattattct
     3961 ttgcgggatt agtctcaata tatacaaagc gatcggctct gtagatatca taatttgcgt
     4021 caaaataaga tgggatgaag ccttcctgga gatatctaaa ttgagcgaga taggagagaa
     4081 tgtcaaaggc gcgccccttt atgcccgcga tagctccgat ggcattgttc atctcatatg
     4141 ccccttctcc aaagggagta agtgaaaatg cggggctttg gataagaggc aaggttatat
     4201 cggctccaac tacatagatc agtcttgtct gctgagccag atagggagtt ccagtatata
     4261 gcaatggatc tctatcgaat acgccagttg ctgccagctg caatcttcca aaaaggctct
     4321 cttccataaa agcaagtggc ctaacataga tcctgccccc tattacatcg aacttagaga
     4381 catttccagt gagtacctct aatccgacat aaggaaaacc aaaaaggcta ccatcgattc
     4441 ccagctgcat gccaaatacg cgccgctgag gaagaaatcg catgttggaa taattgtcga
     4501 caataagacc attaccaagg ctaaaatcgg agatcgagcc catttttaca taaaacggat
     4561 cttcccattg ttgaccatag cggatatata gaatcttagg aagatacaca tcaaaaatgt
     4621 tctggccttg ctgaggaatc cagtcaggct catagatttc tagacttgta ttgcttccag
     4681 tgccaagctt gaatctaaac aatagatcga gcccaatgcc aaatttacca atactgaact
     4741 cgggctgcat acctatttta gaccagcttt cgaacttcga agggtcgcca ggatctggca
     4801 aaaggtccgt cccaaggtta gctattatgc caaatgaagg attcattcct tgctgcgcaa
     4861 aaataggaaa agagaaaaga acaagagcga ataataaggt cgcttttttc atcataacct
     4921 ccttctactt tcctttttaa gcaattatac tatatcaccg attcgattgt atattatctt
     4981 tttcactttt ataggggata gatggatatg ggctttttcg aatacatcga agaaagagca
     5041 gataaaatgg atacctttct atgcattggt ctcgatcctg cgttttcaaa ttcagagata
     5101 gagaaaagag gtagtcttga atgcgctgca tgtgcgcttg agcagaattt gagaatcatt
     5161 gaggccaccg catcttatgc attagccttc aagcccaata ttgctttcta tgaagccatg
     5221 ggtaatgctg gcatgtcagc tctatacaag actctagccc agattcccga agaaattcca
     5281 attatcatag atgcaaagag aggagatatt gcaaacacgg cagaagctta tgctcgggca
     5341 attttcggag agctcaaggc cgatgcggtc acattgaatc cttatttggg tcttgatacc
     5401 ctagagccat tcctgcggtg gagcgataga ggaatttttg tgttatgcag aactagcaat
     5461 cccggagccg gattcctcca ggatatcact gttaacaaaa gccctctcta ttcaatggta
     5521 gctcagaaat gctctgattt atcggtcgat gttggactgg ttgtagcagg taatgatctg
     5581 gaagcgcttc agaaagtcag gaaagttgct ccttcagctt ggtttttaag ccctggtatt
     5641 ggtgcacaag gaggaagagc cgatcttgct tttgccgcgg gcgcaagaag cgacggcaaa
     5701 ggtatacttg ttgctgcagc gcgttctatt tcggaagcac aaaggcctga tgatgctgca
     5761 ctagagcttc gagatgtcct aagaagagca agagacgctt ttttatttaa agccgcagtc
     5821 aaaaccgcaa agccgtctaa gaaggctgaa gatgaagtcg catccctaaa acgcgaattt
     5881 gtcgatgtct tgctttctac tgaatgcttc agattgggcg aatttgtgct taagagtggc
     5941 aagaaaagcc ctttctatat cgaccttcgg aatctaatat cggacccaga agctatgagg
     6001 attgcaggaa gagcttatgc acatctcgca tcatcttgtg atttcgatat cataggcgga
     6061 attccctccg ctggcctgcc tctcgccact gtagcatcgc ttgaacttgg caagcctatg
     6121 atctggcctc gaatgcctgc caaggatcat ggtacgggaa agcgcatcga aggcagatat
     6181 gccaaaggag atcgagttct tttgctagat gatcttataa caaccggtgc aagcaagata
     6241 gaagctaaag atatattgaa atctgaaggg ctagaggtgc gcgatttggt tgtacttata
     6301 gagagaggca gtgagggaag ggtggatatg gagcgggaag gggtaagact gcatgcattt
     6361 atgcatgtaa acgaactctt tgaagcgctg aaagagaatg ggaggattag tgaagcccaa
     6421 tactctgatc ttgtcgattt tgggaagaca atatagcaag aacatggcat cgggttgaaa
     6481 atgcagaaat ccaggcttct gcatctcctg ggatatgcct aaaaggcctt gcgttaatcc
     6541 gatatgactt ctggataggg cttgagagct actcaagaaa agaaagaaca tagatgcagg
     6601 cagaggatat tgtgagtttt tccagctcga gatcatagat atgatctgcc gaaattccat
     6661 gattaataag gaaagacata agagaatatt tcaagccagt tcttacagtt agcacaagag
     6721 cgtcagggct ctttgtgcca agcacaaatt ctagaaggct tagatggcca cctctatgtt
     6781 caagctcgag ggggccgatt tcgtctatca aacaaatttc tctttttttg tcgggtatgt
     6841 tttgaggatg ttgcgaaata atatccgatt caacagtctg aatgtaactt tttcttatat
     6901 gtgcgaatgc atcgggagag tattcaaatg gaacatcccc tctgtctatt ggacgcgagg
     6961 ctgctaggaa agtatctcca tttgacacat tgtaaaggtc tatgctatga ggtattccat
     7021 tctctgaacg ttgcgaaatc tcaatgcagc ccctacaaac gatgccttta tcctctgctg
     7081 acttaatgag ggaattgcaa aatgaggttt taccggaaca gcgcgagcct gaaattaaga
     7141 atattctcat aatctcaagg atggaattct ggcttaggaa agtctggctt ttgtgcgcat
     7201 cataaccgag agtattgcgt atccagaaga caaatcttct ctctgaacaa tcacatcatc
     7261 cggtactctg agtttcacat tggtaaaatt ccagatggcc tttataccag cgtcgacaat
     7321 cgcatcacag cttgactgcg cataaggaga ggggacggtg agaatcgcaa gagttatctt
     7381 catttcgtct gcaatctctc taagctgatt catctggtat acatgaatct gatggatatt
     7441 cttgcccacc ttcaagggat cgctgtcgaa ggcagcgact atatgaaggc catgatttct
     7501 gaattcttga tagccggtta gagcagatcc cagattccct gcacccacaa tcattgcctt
     7561 ttgttcgaca tcccaagcta gatagtgttc aatggctgca attagctgat ctactggata
     7621 gcctttcttg ggtttgccta ctataccagt aatggagagg tcttttctga cctgtattgg
     7681 ctcaaggttt aattcattcg ctatcaaagt gccggaaata tagggttcac cggttctctg
     7741 aaacgcccgt attatatgga gatatgaagg aaaacggcgg atcgaaggga catagttgag
     7801 cttcgttttt tgagacatca gggttcctct aatagctttg catgagagac taattattag
     7861 acttatgtta acctatactt atgtatcgat aaaagtcaag cgattgaatc aacattatcc
     7921 ggtatccaaa aagttcaacc atattataaa agaaaggcgc tgaggaaaag gaggtaaacc
     7981 tcagcgccaa cgctcatgaa aggcatatcg catcattttt cttcaagtgt ttctatccat
     8041 aagtatatca taaggctaat ggatatgcaa cttattgcga ttttttttgc aatttatgca
     8101 aattaacaaa aggaatcgct taggatcatt ggcacactca gtcccaccaa accctgcccg
     8161 aaaaatcagc atggacgcat gaaccggcat gccgaatcga acaagcagtt ctcacttctc
     8221 caaacagcgg agataatctc cttgagaata aatccagtgt attccctctg gcaacatctt
     8281 catcatatag aagaacattc ttattgcgat agtcaaaaag ccatgcttga tccgacaggc
     8341 ttattatagg ttcttcatcg tggcgtttaa acatcgaaaa gcgaataaaa tataccggga
     8401 tttcaagaag ttcggagagt ataagagcgg gcatgatgga accatgagct gcggcgacaa
     8461 tgagactggg tcgaaattct ctggaaattt tctccgcaag ttccaatagg agtgaaggct
     8521 gagaacctag ccatgtgtaa tgatcttgca ctgccgtata caaaaaggaa gcaatgcctg
     8581 atggaattcc aaaacaaagc tcatctatgg ctgatatggc ttgaggagca cattgataac
     8641 acaagcgtgc aacctcgata gattgccagt attgttcatt ggcttgttgg atttcggcta
     8701 gtgaatggcg tattcggtac ttgcgcgcct ctttgagttc gtaatatacg gtaggatgaa
     8761 gcggcaggtc atgttccaca cagattttat agtttagcag tacgtttaca agagctggag
     8821 tcagcagata caagcggatt gccttatcaa gcagctcttg aattctataa gaatcgagtt
     8881 tctcgtcgga cagctgagcg gattcaatgg cagggatgag ctccttaata gtctgaatta
     8941 tacattgaaa agagagggag cagttcatct gttctggtgg agccgaccaa tctgcccatt
     9001 ctagtatctg catgaatttg gtaaaacatg gcataatagt atcctatggc agatacaaaa
     9061 gcagaacaag aagtcttgga catgattgcg gagcaaaggt tgccgttctc agcagctatt
     9121 ctcaatggtg gtcatgccag gcgctttggt ggaattgaca agcaagaggt cgaaatagaa
     9181 ggagttccaa tcggtttttt gctcgcctat agattaggca agattgcaca agagctgctg
     9241 attgtgggca agcctcacaa gttatatgaa gaccttggtg cctctcaatt tgaggatgat
     9301 ataaaggaag gcggtccagt atgtggccta ttctctgctc tagtcaatgc ttctttggaa
     9361 tgggtttgcc tttgtgcggc agatatgcca tttgtatctt tggcattaat tcgatatctc
     9421 tatgttcaat ccatgtcgaa ggaagtggat atagttctct gcaagacaga tgataccctt
     9481 cagccttttt ttgcattcta taagagaaat cttgtctctg aactctattc ttatattcaa
     9541 agcagttctg acttatcctt tagacacttt atcaaaatgg aaaaacatct cattattcag
     9601 gagaatgata tagcttctat ctgcgatcct ggaatcgtat ttcaaaatat caatgatcgt
     9661 aaatcattgg aacgggctcg gaaggtggct aatcgccaag tattgggtct ggatgaattt
     9721 catataatgg aggaataaaa tggagaagcc cttagtagaa tgtgttccaa atttttccga
     9781 aggaagagat cgcgccaaaa tcgaggctat agcagatgcc atcagatctg ttcaaggagt
     9841 ctttctgctc gatgtagacc cgggtgtgga cacaaatcgt acagtatata ctttcattgg
     9901 gcctcctgaa gctgtaaaag aggctgccct ggaagcagcg cgggttgcat ttaagctgat
     9961 agacatgagg acgcaccgtg gagctcatcc acgaatagga gctctggatg tgtgcccttt
    10021 tgtacctgta tcaggtgttt ccatggaaga atgcatagag atttcaaggg acttcggtaa
    10081 gaagctctct gaagagctca atgtgccagt ctatctttat gaaaagtcag cgactcgtcc
    10141 cgagcgccgc agtcttgccg atatccgtgc cggtgaatat gaaggattag agaacaaact
    10201 tgcagatccg gcatggcagc ctgactttgg acccacacat ttcacccctc gctggggcgc
    10261 aacggtaact ggcgcaagag aattcttgat tgcttataat ataaatctca acaccaaaga
    10321 taagagagta gctcacgata tagcactcag tattagagaa tcgggccgca caataagaga
    10381 cagcaatggg aataccatca aggttccagg cttattgcag gctgtccgcg ctattggatg
    10441 gtatatcgaa tcctaccaat gcgcccaggt atctatcaat attctcgatt ttcgcacaac
    10501 cccattgtat gtggtattcg atgcggtaaa aaaggaagcg gaagccagag gaatatatgt
    10561 aatcggttct gagcttattg gacttattcc tcttgaggct atatgcgtct gtgggcgata
    10621 ctatcgccaa aaagcaggca aaagcccagg actttcggat gaagaacttg tcgagttggc
    10681 agtgcaaaca cttggcttgc gctctgtgca gccttttgaa ccagaaaaaa agatcattga
    10741 gtgggcaata agaaaagaag gggctcttgt ctcaaagaag gtcagttctt ttgtggatac
    10801 agtctcttcg gactcgccag cacccggagg agggtctgtg gctgccttgg caggagctct
    10861 tggttctgct ctggctgcga tggacgcaaa tcttactgta ggcaaaaaag gttacgaagg
    10921 tgtttcggag acattatcga cactgggtat caaggctcag agactaaaga atgatcttct
    10981 cgaaatcgtt gatgaagata cggcagcttt caatgcggtg atggatgcta tgaggcttcc
    11041 aagatcaacc gatgccgaga ggatagctcg agatttggca attgagaaag ctaacaaact
    11101 ggcggctggc gtgcctctta agactgctcg gttttgtctt gaggctatgc gcttatgcct
    11161 ggaagcagtc gagactggta acttgaattc agctaccgat gcagccacag gatgtctcat
    11221 ggcatgggca gggctttctg gagctgtttt gaatgttcga atcaacataa agacgttgtc
    11281 tgatgaacaa tttgcctgta cggcaagcaa agaatgcgat tctttggaaa ttgaagcaac
    11341 tcagcttcgg caggatcttg agaagagcat tccgagtatt cttggacttc gataggttta
    11401 tttcgatgag ctacattttt tcaagacgat ccaatgctgg agaaatagaa cccaatgagc
    11461 tctcgagggt tcgcgcccag aggcatggtt ttggacttcc gataaaagac ctatctaata
    11521 caaatcccac aaaggtaggg ctcgattgga aagatctcga tcttatttct ttggcaacca
    11581 aagataatgt tacctatgat cctgaaccta aaggtcttta tcgcgcccga gaggcgatct
    11641 cggatcattt tgctttatac aaaaggaata tgcatcccga taactatttt ctatgtgctt
    11701 ccacttcgga ggcatattca tggcttttca agctgctgtg cgacccgggc gatgcggtac
    11761 tcgtacccaa gcctggttac cctctctttc aacacctggc agaactggat tcggtacaag
    11821 tgctggccta taggcttgag tatgctcatc cttctggttg gtatgttaac ctagatgagc
    11881 tttcttctgt tttagcagcg gaccgtgaaa agcggataaa ggccattgtt ataataaatc
    11941 ccaataatcc taccggttca tacattcgaa agactgaaag aaccacaatt ataggactat
    12001 gcgaaaaata tggtctcgca ttgattgcag atgaagtctt tttcgatttt cccatagacg
    12061 ataatatcga acgaaagacc ttcgtagggg aggatagggt gctcaccttt gtactcgatg
    12121 gactctccaa acgaatgggt ctgcctcagc tgaagcttgc gtggatcacc ctatctgggc
    12181 caaaccaaga ggtatccaga gcaaaaaaga ggctcgatct catcgcagat acttttctct
    12241 ctgtgagcac tcctataatg aatgccctgc cttttctatt tgaacatgaa agggcttttt
    12301 tatgtaagtc gctaagcaga attcgcgaga attatgcaag ttatcgagaa attctaacag
    12361 gtggcaccag ccctcataga gtgctttgct gtcaaggcgg atggaccgca ctaatcgaaa
    12421 gccctagctt ttttgaagaa gatagaatag cccagcgcct actggaggag aaagggattt
    12481 ttgcgcaacc aggatttttt ttcgatattg ataatggagt acattttgcc tttagcctga
    12541 tcattccaag catcagcgca tttcgctggt gcacagaata taggcatttc tttgaagcgt
    12601 tagagaattc atgacgaaat cttagaaata tagtatcttg aagatgggag gacttatgtc
    12661 tgcagtactt atcgatggaa agaaaattgc tgaggagatt agaaatgagc ttgtccaaaa
    12721 agtcaacttc cttaagggaa aaggagtcgt gccaggtctt gctgtgattc ttgtggggga
    12781 taatccagca tcgatctctt atgttaccgc taaagaaaaa gcctgcgcag agatcggtat
    12841 gaagagcttt gagacaagaa tggcggagaa ttcatcggag caagctctcc tcgatagaat
    12901 tatggaatgc aatagagatc ccaatataga tggcatcctg gttcagcttc ctcttcctag
    12961 gcatatcgat gaacgccgtg ttatagaggc catcgacccc aataaggatg tagacggctt
    13021 tactccagtc aatcttggcc gcatgctcct ggaacaaccc tctttcatac cttgtacacc
    13081 atttggaata atagaactcc ttaagaggag tggcgtgcca acaaatgggg caagagcagt
    13141 ggttgtcggg cgctcgaata ttgtgggtaa gccacttgca aacctccttc tccggaaatc
    13201 tgtcaacgcg acggttacta tctgtcacac agggacgaaa aatcttggct ctcatgttcg
    13261 cgaagcggat atcgtggtgg cttgcgctgg agcacccggc ttggtgtctg tggatatgat
    13321 caagccaggc gcttgtatta tagatgtcgg agtcaatcgt gtgccagatt ctactaaaaa
    13381 gaagggttat cgactctgtg gtgatgtcga ttttgaagga gcttcgagga ttgcaggctg
    13441 gattactccc gtgccaggag gggtggggcc tatgactatc acgatgttac tctcaaacac
    13501 tatcgacgcc gcggcgagga tttccaatat cgcgatttga aaatcgaagt ttgcatgaga
    13561 tagagagaat atgagtttaa gatatttgta cgtgctctga tagggaatat gggttagaat
    13621 catggtagat gttcagtcgt ttagggatga tcgaaatgtc gcaatccaga aggttggggt
    13681 caaaggcctc cgatatccca taactctgct tgataaggcc gaaaaatacc agcatacaac
    13741 ggctgcggtc aatttatttg cagaccttcc acatgatttc aagggtactc acatgagcag
    13801 attcatagag gtctttgagg aataccgcac cgacctctcg atgcccaatg tgataaaaat
    13861 gctgagaaag ttaagagcag aactggatgc tcagacaacc tatacagata tccattttcc
    13921 ctattttatc aagaagaagg cacctgtctc ggaacaagca tctattatga actatgaatg
    13981 cttttatgaa gcaagttgtt cggggaaagg acatcgtttt attgccggcg taatagtgcc
    14041 cgtgcaaaca gtctgtccat gttccaaagc aatttccgat ggaggagcac acaaccagcg
    14101 aggcattgtg acacttcaag tatctcttgg cccatttttt tggtttgaag acctgatacg
    14161 aataatagag gaatcggcat cgagcgagtt gtttactctg ctgaagcgcg aagatgaaaa
    14221 gtatatcact gagaaggcct atatgcagcc tcgctttgtt gaagatgtcg tgagggaagt
    14281 atatttaaaa gttgatgctc ttcaacgatt tccatggttt tcggttgaag cggaaaattt
    14341 cgagagtatc cacaaccatt ccgcttatgc atatgtcgaa aaaagcgatc gctcgaagat
    14401 tgaacctgtc agtggagaac atggaaggct ggatcgactt gaatcttatt atccttgatg
    14461 tgaggaatgt cgattagagt taataattct ggagtatgat gcattttgga ttaagagagg
    14521 aaaagatatg gaaagcaatt atccggttga tcccaagact ttgaatgata aagggacaaa
    14581 gggtgttatg tcttcggttg caggcctagg ctttttgggt gtcgaagctc tgcttggtat
    14641 ccccgtagtc ggtactgtga tatcagcagg actcataggg cttggcgcta taggtctttt
    14701 aggaaagaca aagaccgaca agatttccgg tacagtgcta ggggtagctg gcattgcagg
    14761 acttaccact ctttttctgc caggcgtagc acattcgctg ctttccctgg gtggcatcgg
    14821 acttcttgga tatggccttt tcaatcttgc tggatttatt agaggcctta aaaagaagaa
    14881 atgaataatt atcatatcga gacctatggc tgcgaaatga acaaggctga atcggcagcc
    14941 atggaagcga cccttagaga acatggatgg aagcaatctg aagagagaga agctcagctt
    15001 atcttattga atacctgtac tgtaagagca accgctgaaa accgagtatg gaatcgtata
    15061 catcaactga ctgcgcgcaa gaaagaacat gatttcgttc tagtggttat tggctgtatg
    15121 gcggagcagt ataaaaacgc cattaaaaaa aaggctcccg gcgttgacta tattattggg
    15181 acatatcaaa ggcaatcatt ttcgctaatg ctcgattcca ttgccaatgg ccaaaaaatc
    15241 gaagtgcttg aggagaaccc catatatatt tttagtgagg accatcacga gactggcgca
    15301 tttcgatcct ttattcccat cattcatggc tgcaacaact tttgcaccta ctgcatagtg
    15361 ccttatgtgc gaggccgcga aatctcgcgc agtcctttgg acatacttcg agaaatagat
    15421 agactagaaa atcttggtgt tcgtgagata attcttcttg gacagaatgt aaattcctat
    15481 acctgggaaa aagatggggt atctttggac tttcctgggc tcataaaaat gattgcagcg
    15541 cacctgcgaa aaaagcaaga gagcgaagga acgatagaat cctacaatgc atcacctaaa
    15601 aaatctaagc gcggaattgg ctggatacgc tttattacaa gtcatcccaa agatctgtct
    15661 aaagagctca tggcagtcat ggcagaggat ccttttttct gtcatcatct ccacttgcct
    15721 ctgcaatcag gctctaatac catactctca gcaatgaatc gcaaatacac tcgagaatac
    15781 tacctttctg tcatcgatgc tctaagaaat gcaattccag accttacatt gtctacagat
    15841 ataatggtcg gctttcccgg cgagaccgaa gaggatttcg aacagacatt agaccttatg
    15901 cgcagcgttc gtttctccta tgcttttatg tatcacttca acgaacgcaa tggcacattt
    15961 gctgcaggaa tgccaaataa gataactgaa aagataaaga aagaaaggct cgcccgggtc
    16021 attgctcttc aaaaagagat aacctcttct cttatgagag gacggattgg ccatgtcgac
    16081 gaagtgctca tagaagatcg gtcaaaacga tcaacaagag agatcttagc gaggacttca
    16141 cgcgatgaaa tggtggtctt tcctggttca gcgacaagaa ttgggcaatt tgcccaggta
    16201 aggttcatat cggttgttgg caatacatta agaggcgaag aagtgtaaag aggaaaagtg
    16261 tgaaaagaaa taggataggg ataacaaggg atcgctcttc gctaaatttc gccgcctcat
    16321 ttgtactttt cctatcatta ttcattctta tcattaactc atccatagaa gcggaaactt
    16381 acaaaagcct gagtcaagca cggcaggctg cgtccaagac taagagccaa gatgctttga
    16441 gggatgtctt gctttcggca gtttcgcagc tatctgtcag agatggaatt gcgctttgtc
    16501 aagagtatga ggcaaaaacc gaccaaaact tgaaagccga gctcaggggc attttaggtg
    16561 ggctatatct gcttcttggg caaacgaaag aagctgcttc gtggtatata aaagccgctt
    16621 ctatcgacgc aaaatacact aatgaggcgc taagactttc aatagcaatc ggcgatcaga
    16681 aaaatgcatt ggcgctcttg aaaaatgagt ccatttcgcc tgaattcaag tatatttttg
    16741 atatttggct ttctctctat gatggagaat atgcgtcggc ttcttcaaaa gcgagagatg
    16801 ccctggctat tgttacaaag cctcaactgc gaagggagct acttcttctg caatattttg
    16861 cggattttgg tgagtttggg gctggtattt ctagcttggt caaggatttc cctaactcaa
    16921 tagaatcgga tttaattata ggaagagttt ttccttcttc atcgcttatg cttcttttgg
    16981 gcttttcgtg gattaagggc ccagcgcctc ttgttgatta tggagtaaca tcgaacaatt
    17041 ctcaaatagc ggaagaacat agcgccgcaa gcggaatcca atggctacaa gcaggctatt
    17101 tttcatcgaa agataacgcc gaacgtctct ccagaaatct gactaatcag aagttcaaga
    17161 caagagtcat agaattgaaa aaccaagccg gcgaaccaag atgggctgtc catgtggcag
    17221 ctgaagaaga ttggaagaag actcaatcca agctcaaaga ccttggatat gaatcttatc
    17281 ctgcaggcaa ttaacttgtt ttgatgaaga tgtagggtgt tcgatattcc attctgacgc
    17341 catccgcccc tatctcagct ggtctgagga tcaggcgatt gattttgcca ttctcggatt
    17401 gatcgatgga gacaatgaag agcaacctat ctccaacaag tccattcgac tgaatctgca
    17461 agaaatcaag cacctggtat ccatcataat agtagattct tgctgcccct tctctatatt
    17521 tgccatttaa tacaaggatg ttcttagact gatcgagaac tagcacagaa tggtcatcac
    17581 caagatagcg gcccaggaat tgcgaatgat cgggacttga gagaatcgtc ttattgtcct
    17641 tagcttcctt catataatca taggattcat atttcccaga ccaaccctca tcgcgacgga
    17701 aatgagatac ttgttgatcg attatgctga ccgatatcac gttttgtcct tttgcgtcta
    17761 ttataaagat tcggtcgaga taactcagtt ctgagagcct acaggtaaga tagagtcgat
    17821 tgcctttgcg ttcggcactt ttgattatcc atcgctgctg aatctgttgg ccttccaaga
    17881 tcagtgcatt gctattcgta tcgagataca gcgagcatat attggaggca tctgagttct
    17941 ttttccaaaa ccgacttagc caggatagat atgactgagc atcgcttcca gaaggacctt
    18001 ctgagatgct attgctctct cttaagggag attccgatac tttctccaag aacaggctgt
    18061 ttctattcca tatataggtc ttataggaaa ctagacctgt tttatttggc aatgattcga
    18121 caacgataga tggtatagca gactcatcag agtcctgaag atttatggat aggcctgtct
    18181 ccgaaaatat gagagaatac cctcgattta atagccgacg gaatatggtc agggtttgat
    18241 tgttagagct atccaagccc tggataagaa gatcagaaag cccatcacct gtaacatcaa
    18301 gtggctgcac ggttatgctc tctagctttg aagcccgtat ctccgcttca aaatgcctaa
    18361 ggtaagcacc aagatttggc gaaaagtcag ctattatcat ccacaagacc gtgcttccag
    18421 ccgttttctt gcatagaata acttgctcaa aatcctcatc atcgtcgaca tctatgtcta
    18481 tggtcttaag aaagatatca cccggaagag gtgatatctt tgcatgctgt agtgaattat
    18541 tttgaggcga aacctggctt ttatccgata ggctttcgag gacaatagtt cgattctcta
    18601 ttggcaccga agcctgtaat gtctcttgcc tttgtatgac aatcaggtat actgcaatca
    18661 aaaaggccgc aaaaaaagcc acaaaataca aatggctgac caaggatttg aaattattca
    18721 tcatcctctg caaattagat tctaggctct gtccagcacg tagattattt tctcaagagc
    18781 ttcttcgagc tcgtcctctg ttatgacgag cggtggggca aagcgtatta tatttccgtg
    18841 cgtctgttta gcaagtatgc cagattcctt taagtctatg caagtaggat aggcatcaaa
    18901 gccctcctcg aagacaactg catttaggag accctttccg cgaaccaagc gaaaatgggg
    18961 tgattttgct ttttttatga cttccctaaa gcgttctccg agcctaaatg ctctttcatc
    19021 gagattttct tgttccagaa cttctagagc agcaatgccc accgcgcaag cgagcggatt
    19081 cccgccaaaa gttgagccat gcgtcccagg tgtgaataca tccataactt catgatccgc
    19141 aacaatagcg gaaatcggaa tgataccgcc tcccagagct ttccctagac acataatatc
    19201 gggacgggca ttttcgtaat tccaggcgaa atggcggccc gttctgcaaa aaccagtttg
    19261 gatttcatcg agtatgaata agatccccgc ttcggtgcag attcttctga gatccgagag
    19321 atagccatcg ggaggaacga caacaccggc ttccccttga atgggttcta ccaacacagc
    19381 aactgtattc tcatctatgg catcggagac cgccttggca tctccaaaag gaatctttat
    19441 aaagccaggc acataaggtc catagtttat ataggaatca ggatcacttg acatcgaaac
    19501 agcgcttagc gttctaccat ggaagttgcc attggcgcat attatggact gtcttccatt
    19561 ttctatccct cttttttcta caccccatcg ccttgcagcc ttgattgccg tctctacagc
    19621 ttctactccg gagttcattg gaagagccat ttcatagccc gtatactcac atagtttctt
    19681 gagaaagggt cccattttat cgttgtggaa ggctcttgaa gttagggtca ctcgttcgat
    19741 ttgacttttg acagcgttga caatgtgtgg atgcagatgg ccttggttta gtgccgaata
    19801 tgcggagaga aaatcatagt attctcgccc ttcgggatcc cagaccttgc atcctttggc
    19861 cattgaaatg acaacgggga taggatgata attatgtgct ccgtattggt aatccagagc
    19921 tatataatcc tgtgaattca tgatttcctc cttttgatat tattggagtg gtctgtcttt
    19981 caatgctaat tataacccga ggcaattgaa tgcggaagtg ataataataa ttagaaatag
    20041 tataaataat tatgcattaa ctaaaactga atttttgggt aaagctgact catggctaat
    20101 tcctgattct gagtctaagg agatgattat tagaaaattt tatgcttttg agaaatcata
    20161 ttcgagctca cgctttatcg catattgttc tatcgcaagt tcaaggagtt tatccagcaa
    20221 ttcagaataa gagactcctg catctatgca cattcgagga tacatagaaa tggcggtaaa
    20281 accaggaata gtgtttatct catttaaaaa tacttctcct gttatcttct ctacaaagaa
    20341 gtcaactcgg gccattcctt ttaccgaggc atatttatat gcagaaagcg caaggctcat
    20401 tattcgattc tttgtctctt ctaacaacgg agcaggtatc aaaagggtag cgccctgtgg
    20461 atccttatac ttcgcttcat agtcgtagaa ttcgtgagat ggaacgattt ctccgggagg
    20521 aaagaccata ggtcgctcgt tgcctagcac cgcgcattct atttctcgag cattgataaa
    20581 tgcctcgatc aatgcatgct cagaccagcg aagggcagac agtattgcat cttttagctc
    20641 ttctgaatga gtgactttag aagttcccac cgaggaaccc gaacatgcgg gctttacaaa
    20701 acaaggccag ccaaatcttg tttctacttt gcgtgcaagt attttaaggt tttcatccga
    20761 aacatcttct tttctaaccg aaatataatc gaccaccggt agaccagccg acagccagag
    20821 gcgtttagaa acttccttgt ccatgccaac cgcgcttccc aaaacatccg ctcccacata
    20881 aggaagatct accgtttcga gcaaaccttg gatcgtccca tcttctccaa aagtcccatg
    20941 cagcacggga aatacaacat caatattgag tttttgcagg ccttttgttg ttgtctcgag
    21001 ccaaagtcct tccccaggca ctgctaagac gcgttcagat ctttcgatgg gaggaagaga
    21061 gcgcatttgc ccattttcgg gcctggaata agcccattct ggaggatgct ggaggaacca
    21121 ttctcctctt ttggttatac ctattggtac aatcgaatac ttatccttat cgaggtaggc
    21181 caaaatgctg ctcgcggata taagcgatac ttcgtgctcg ccggatctac caccatacag
    21241 tacagcgata ctcttcatag attatttccc tttatttgtg atttcaaatc ccaagtcggc
    21301 caggatcgat cgagcttgcc tttcttcatc ataggggata gtcctgtctg aatagattat
    21361 tgaattctca tgacctttgc ctagaagcag gacaagatct ccgggctttg caagattaaa
    21421 tgctcttcga attgcttctg ggcgatctgg tatgagatat agtcggtctt ctcttgagag
    21481 ttcaggacaa cccatggcta tttcttcaag gatggccata gggtcttcac ctcgaggatc
    21541 ttcatcggca agaatcaata tatcgcaata atccgcagca atgcgccctt gctgtggtcg
    21601 cttttcggtg tcgcgttcgc cagctgatcc aaagacacag atgatgcgac cttttctttc
    21661 ttttctcaga gaggggagta tttccttaaa tgacgatggc gtatgggcat agtcaattat
    21721 cacttcgaaa ggctgtcctt ctagaattcg ttgcattctg ccttttatcg gcctaaggcc
    21781 tggaagcaga ggaacgaagt tgtgccaatg caagcccgtt gcggcggagg cagctagaat
    21841 tgctccaagt gcattttgaa cattgaaggt gcctggaaga ttgatccggg ccgataatgt
    21901 cgaaggcata acattttcga atcttcgagc agatatttct tgttctgtgc ggaagttgtg
    21961 tccgataggc atttccagaa gcccctctat ctcaaaggaa actccattgg catcttctat
    22021 tatattgtgt gccgagagat ctgctagggc tcccttgctc gaataggtca ggcatggaac
    22081 cgcagactca tgcacaaagt atgatgcatt tgggtcatcg gcgcagataa ttccagtagg
    22141 agagataatt atattgctat tatgttcatt tatcgcatga ctaactttat tgagtttctt
    22201 tggttcgacc tcgccaaggc gccggaaaag attcgccttg tcgctgcgat actgctcaca
    22261 tgagccatgg aattctaaat gctcatgagt tacattggtc acaaggccaa tgtcgaaatg
    22321 gacatgggct aaccttgcgg ttcgtggaga taagccatgg cttgatgcct ctataactgc
    22381 aaactgtaat ccatggttgc ggatttctgc caacatctgc tgtactgtgg ttgcttctgg
    22441 agttgtctgg tgctgaggat ttggctgttc cccttctcca atatctgaca ttacagtgga
    22501 aaagaaacca gctttgaaac cagcagcatt cagaagttgg tagataaacg atactgttgt
    22561 gctcttgcct tctgtgccag ttacaccgat aacgcataga gaatcagatg gattctcgaa
    22621 aaaacttgcg gatatagccg acatcgtcca tcggcaatct gtgacccgga tcaatgcagg
    22681 atttagagct cttgccgatg gatctttcaa cataaattcc ctcaagttct tctctagaag
    22741 gccttcatag atcacggcaa ctgcaccatt ttgtatggct tggataatgt attttttgcc
    22801 gtccgtatgg aggccgggaa gcgcaaaaaa cagggatcca ggcttgcatt ctctcgaatc
    22861 atagaaaagg cctgatatca aatgatgaat atctccttcg attgagcaga tatcgagtcc
    22921 ttgtatcagg tcagacagcc gtttctgcat attgtgtcga attctatcat aagataggag
    22981 agagctgcaa tgaatcttaa caatcttaag gcaagcattn nnnnnnnnnn nnnnnnnnnn
    23041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23101 nnnnnnnnnn nnnnnnnnna ttatgagcgc accgacaaga tgtttttcga aaaatcatat
    23161 ataatatgga gattggaaag ccccgtttcg gccatcatga atagggtttt taagctcaat
    23221 gatttctagc ccattaaagg aaggctaaaa tgtatcatat tagagtggaa actgagtttt
    23281 ctgcagctca tagacttttt cactacaacg gaaagtgtga acgttttcat ggacataact
    23341 atagagtaag agtctgggct tcgggcaaag agcttggaga gggtggaatg ctaattgatt
    23401 ttggcatatt aaaatcagca ttaaagaaat tgatcgatga ggaatttgat catcaagatt
    23461 tgaatcaaat agccgaatta ggagaggatc ctagcgctga gagaattgcc actctgatct
    23521 atggtcgatt acatagagat ttcccttcct tgcccatctc ggccgtcgat gttttcgaga
    23581 caagcagttc tatggcgcgt tttgtgcccg acgcatggcc tgatgctcga taaatcatgc
    23641 cagacatacg ttcgatagat cccacccctc gattcgaaat attgtctctt cgagagtgca
    23701 ttttttatca tagagaaaat ccgccagcaa tgctatatcc gccttcttac caatagatag
    23761 gcatagagat atattagtat aggatgtctt atattgtata tattgaacag atttgttagc
    23821 tttatgagct gaataagtct gataaataat actatcttca tctttttgca atgtaaaatg
    23881 tagattcaac tcattgagcc atccctcaag gacctcatta agaaatccgt ctctatttgc
    23941 tggaatacaa aaggaaagcc tgcaggtttc cgcttgagga agctcttgaa ttatctctat
    24001 ttcttccagg aaggctgttg atccagaata gttgcttgaa attgaagata gtccgatctt
    24061 tttggtctcg ttggaatgct ttatcttcga tatagcaaga ttgatgattc tctccatgtc
    24121 cgttttatcc ggaccataga tggcgaagta tttcactcct gatatgccaa tcctcaattt
    24181 acagggcata gaggcggatt ttgtcggaat agcgctttcc caatcggagt ttgggagctg
    24241 caatttgcaa taaaaaagag ctcgttccgc atttgggacc aaaaaagata aagttctaag
    24301 aagccaagcg cgttcataag ctggagtcga aaaagccagt gcatcgttct cccttattct
    24361 aacaagaatt gaaggaaaat gagccttcgc tttttgttgg gccttgataa tgtcgatagt
    24421 tctttctgct tctagcgaaa ttagcggaat tgctggcatt tttgtataga actttttaga
    24481 atccggagaa catagattat cgatggatcg atcataaagc tctgcgagca tttgccagtg
    24541 tctagcgctt tctaaaaaat caaaatcagg acgataggta tgaggctttt cctgcaatag
    24601 ttgggtaaag caagactctc tattgaagaa agcacaaagg ctattcaatg cgcgatcaga
    24661 aacatgaaga tcatataaaa acccattctc agggcaagct tggagccaat catgcattct
    24721 tgaaccgccc aacgaaagca gctgatccat ccaataatcc tcgaaagagg atgctagtcg
    24781 gaattcaaga tttgatttat tgcagctaat gcgaagggca gatgcgatgc tctgcagtag
    24841 agccctatct ccttgcaatg gagcatattg cagaggtgta aagggcaacc tgacaagata
    24901 accggcagac acgattactc tagtaaccga ctgagtttct attttatagt gggctagttt
    24961 atcgcagaaa ttttcgaatt cttctaagtc atgtccattt tcgaatcctg atataataaa
    25021 aaagagcttg atttctcgtg ctttattatc gatgattctt gttatggaat tccaaatctg
    25081 cccttctgat ataccttttt ggtagtattt acgaattctc tcagaaattc cttcgatgcc
    25141 aagggcaaaa gaacgctttc ccgcagccag ctccgcggca agaatttcag gtttattgga
    25201 tagtatatcg atgcgctggc tcatcatgga gactttcata aaatagcggc ctaagatctg
    25261 gattagctgt attatgtcct tatgcatgtt gaaattgtag ctgaagagct caacatcgga
    25321 ggcgccgctt gctcttttga gtacgactgc cgatttggac aaatagtcgg agggcttttc
    25381 actataaggt cttctatccc aaccttcgag gcaaaatgtg cagtggccta tgcatccagc
    25441 gctaattgcc agcttgacgg tatcggcatt ttcgctattc agcacaatag gcgatgtaag
    25501 aactgccggg cgctgaaaag aaagtgctcg attgcaggta aagcttgaat cacaaggcca
    25561 gaatccctca acttcagagg ctatgtgggc taggatcgac agttttgata agcccgtctt
    25621 tgaatcatca gatgcgatgc gcacaatctc cgatattctg ccttctccct caccaaaaaa
    25681 tatggcgtcg acaagactat tgataacttt gttgtcggag attttcacaa gcgaacctga
    25741 acagactgcg ctcgatcctc ctaagaagag aaaaggcaga tcatatcttt ctaacctttc
    25801 atgtcttgaa aaggggattc cggattgaga gagtagccaa gggatgttta atagttccag
    25861 agaaaatgca caagatatta gaagcatatt gaattctgtg gcactcctat tggatgcacg
    25921 cccaaaaaac caaggtcttt ttgcaattga aagggctttt cgatgagaaa agctcggcat
    25981 aaatgcaaag tcgatatagg ctttgggaag agcatgcctt gcttcatcaa agagtacctg
    26041 gtgcgtatac gaaccttcga catcgcgaaa gggagagagc ctaactatca gaatcctata
    26101 ctgagcatcc tcgaatcgcg gattggctat atctgcagcc actgcttctg tgacaatttt
    26161 gggttcactg attgtagatg cactcataga tggctcttcg agcgcttttg tgtttccata
    26221 tcttcaactc gctcgaaaaa tgcctcgaca ggtggttcca ttacggttcc gtattcaagc
    26281 cattttgtac gaaatacaaa gccaaatcgt gttgcggtcc ttgaggcgcg ttcgagttcg
    26341 agcttatccg cgatcagaat gcttatgccc tttttccccg ctcttgccgt acggcccgct
    26401 ctatgtacat atactccgcc gtcttctggc aaatccaggc ttatcacatg tgttatatca
    26461 ggtatatcga gacctctagc ggcaagatcc gttgtgatga gcagagaaat accgcctgtc
    26521 ttaaattgtt ctattatgga atgccttgat tgcttttcca ttctagatac aaggctggcg
    26581 cattgtagtc cggaagagtt caagcgttcc gcagcccttt ggacgcgata agcttctgat
    26641 gcaaatatca aacatcgttt cggatgtatc gccactatta ttctcttaag aagatctatc
    26701 ttctttctgt gatcggaata gaatgcccaa tgctcgatgg cttgagatag tactccttcc
    26761 ctttcaagtt cgattacgat gggcttgggt atccatggtt ttgctcggtt acgagttcgc
    26821 tccgggatgg tagcagaggc catcacgtgc gtgcagtttt tgggggcatt ggagagaaga
    26881 aattcaactg gttctatgta ttctctggag aatagcctat ctgcttcatc aagaataagg
    26941 aattggagat gtgaaagatc gaaatagcgc aagcgcgtta ggtctgcaag gcgcccaggt
    27001 gtgcccacta ttatatgagg gtgtattttt agcgcttgtt tttgacgcgt aaattgagtc
    27061 cctcccaaga ggacaagact ccgaacctgt ggagaattca aaagattcga atcgatcttg
    27121 ggagctcgat tgcgtaattt acttaatcct tgctgttggc atgaatcaga ttgcgacata
    27181 gcaccgttat tttttgactg cagagccatg gctagctgag ccgaatgatt ggctacttgt
    27241 accgccagct cctgggtagg acagattatg actgctacgg gccatcttct atcagcttcg
    27301 tcgatatttg acaatatggt attgtagata ggcaaaagat atgcgagagt cttgcctgtt
    27361 cctgtctccg attgcatgct taggtccttc ttttcgagaa ggaggggcac tgcctgcttt
    27421 tggacttccg tcggctcatt aatgccaagt atttctagag cctgtatgag actcgtgttt
    27481 atccctaagc tcataaaatt attcatgcta taacgctata cgaaaagtgc tgactcttgc
    27541 aagacttcac tgcatctctt tggggggtac cttcgggggt cacgacaatt ttatgcaaag
    27601 gtagctaatc agcatatctt cgagcccaat aatcctcatc gggtgttcgc ttgccctacg
    27661 agatttgtga gagtatagtg gattggaggc cttttgacat gcatgtcctg atatcgaata
    27721 gcggttctat tgagctggac gcgcttacca gaagcgaatt caatttttct gtagaccatc
    27781 taatggaaat tgccgcaatt cgcttgtggc agaccctttt atctgaagta attgtcaaga
    27841 agaatatgtc aagcctgatc tccgctggat catcatcttc tcgtcagcct ggcatagctg
    27901 cagtatgtgg aaaaggcgac aatgccggag acgccctggc aatgttgcgg catgctagat
    27961 tggaaggtta ttctcggctt actgcatata tacccgaacc caataccatg aaaaatgaag
    28021 tcatctcaaa cttgcgtcga gcagagcgat gtggaacgga gatcatctgt tatgagacaa
    28081 tcgacttaaa aagcatcggt gaacggcttt ccacagagga tattatactc gacgccttgt
    28141 ttggaacagg aatagaagga cccgccaggg ggagagcttc agatttgatc caaatattgc
    28201 gagatgtaag aattgctcgc aatgaaaggt gcaaaaagaa ccctctcatc gtatcggtag
    28261 atgttccttc cgggcttagc gatggatgga gaccggagtt tccgattgtc tgcgccgata
    28321 taacgctctg catagagcca atgaaagaat cgttatatat gccgaaagca agaccatacg
    28381 ctggagacat tattccagta ggaagtattt tccccttatg gacgaaggca tcaacgcaga
    28441 aaacatggct tttggaagaa ggagatatga agggcttctt gccccccatc tctccatggg
    28501 catataagat gcagcgaggg cgagtagccg tgtttgcagg aagcgccagc ggggcagggg
    28561 cagctctgca ctgtgtacgt ggtgcagcat ctgctggagc aggatatatc gccttattct
    28621 gcgatgaaga actattcgca gcttatctag cggctatagg agagagtgca attgttcgcg
    28681 tattttcgga ggattcattt tcatcggaat gctgggatgt tgtgatagca ggtcctggat
    28741 ggggaatcga tccgggaaga gagagaattc tggagatgtt gttgcaagca catataccgc
    28801 ttgtgctcga tgcagatggc gctcggctgt ttgcaagaat ggcaagagca aaccaggatt
    28861 ctagcttttt tggagggccc attatactga ctccgcatcc cggcgaattt agcgagttca
    28921 tcccaatgct gggttccgat ttttctggta agacttcgga gaccgtggca tcggtaagcg
    28981 ctcttgcaaa aaaatatgaa atagcgcttg ccctaagggc ttctacgact catgtcggtc
    29041 ttccggatgg tacctgtttc atatatgatg gcagtactcc gggcttagga attgctggat
    29101 caggagatgt attgtctgga atgatagggg ggtttcttgc tcgatggata gctttctcaa
    29161 aggaaagaaa agaaaaccga gaatcgacag aacctgccac ctcttctgat aatcctgtgg
    29221 cgagagcatt attgggggcg atattggtgc acggcttagc tggaaaacag ctttggctca
    29281 aaaaaggatg gtttactcct ttagacctag ccaatgcctg tgctcgcata agtagcggag
    29341 ccatggaaac cgatatggat aatgatagat agcagtgaga aatacagatg gttggacaag
    29401 atgatacagc aggtaaaatc gaatctctcc gctcagtagc tggcattgcg attaagagcg
    29461 atctattgtt cgttgcgaga cgcaagaagg atcatacaga aatgagccac cgctgggagt
    29521 ttccaggcgg caaagtggaa gaaggcgaat cggatgaatc tgctctcaag cgcgaattcc
    29581 tcgaggaatt cggcgctaat atcgatgtaa taaggttttt aggcgagagc attttcgaga
    29641 ataagggaag aactcggact ctagccgcct gggaggtctc ttttagccct gggagcatcc
    29701 gacaactcaa tgagcatgtc gaagtagcat ggcttcctat agaatcgatt tcgaatatgg
    29761 aactcgccga ctccgaccgt tccctgattc ccattttgga agcggccgtc aaaccaggta
    29821 gttgaggtct attatggcgc ttgcttgtct tgagagtcat tttgagactc ggaagactga
    29881 aaatcgaatt tggtaatgtt gggcacttca gcctcatcgt tttctctgac ctcaaggcta
    29941 ttgaggagga tttctacctt gccctgtagc tcatctagtt ccttcctaag ttctcgagcg
    30001 agcattacac cttcttcgaa aaagccaaac gatttttcca aaggaagatc cggatctctc
    30061 attttctccg ctaattcctc gagatgtgca atcctttttt cgaaatcttt catcctgaac
    30121 tccttttgtc accataatct ctggtcgctt ttacatcagc atccgcttca ccttcataga
    30181 acatgatgtt tatagaatcg ccaagagcca gcattctgga agagtgtact atatcaacgc
    30241 cgttcttttt gacaactgag aaaccacgtt tcattatttc cctagggttg gctatttcta
    30301 tagcgctcga agcaaggacc aagcggtgat tcaggtcttc tgcttttttc tgaaaactgt
    30361 tccttatgct ttcgatagcc gaatcaaatc tctggcttgc taccagattc tttcgcagga
    30421 ggatgtcttc cataatatga ggagaaatag aagaaagtct ttgcttcgca ttggcgatgg
    30481 agtgcttcat cgattgcccc atgatatttt caaaatgagc tatctcctta tatatcgaat
    30541 ataaatcagc gcatgccatt tcggccgcgg ccgaaggtgt cggagcccgc agatctgccg
    30601 cataatcgga aagagcccag tctgtctcat ggcctactgc acttattatt ggaatgttgg
    30661 attctacaat tgcgtgaatt actacttctt ccgaaaaggg cagcaaatct tcgatagaac
    30721 ctccccctcg tccaacaatg agcacatcgg ccagattcca gaggtttgcc tgtcgaattc
    30781 gctccgcgat tgaagcggct gcttcttgtc cctgcaccaa tgttggaagg atcaagatac
    30841 tcaccttagg attgcgcctt tgaatcacac gaattatatc gcgtatcgcg gcgcctgtag
    30901 gacttgtcac aactcctaat ctggtgggaa aggcagggat ttcttttttt ctggattcat
    30961 cgaacaggcc ttcctcggcg aattttcgtc tgcgctgttc cagcatggcc aagatatcac
    31021 caaatcctgt ctgcctcaca ttccttgcaa tgagctgata ttgtcctctc tgcgcataga
    31081 ctgatatagc tccgcgtgcc catacttgca taccgtcttt ggggacaaaa tcaagggtaa
    31141 gcaaatccct tcgaaacatg acaacttgaa gaatggcttc ctcgtccttg agagaaaaat
    31201 agagatgtcc ggaagctgcc atcttgcagt tggaaacctc tccctcgatg atgacatctg
    31261 ggaatttgtt ctctaagtta tttcttataa gagaagtgat ttgggagaca gaaagcggag
    31321 agaatagacg gctttcggca tcaagattgg aatcgaagtt catactcgga ttttatcaga
    31381 atgtttctct tgggtgaatg tcgcatactg gctgaattct cttcatatca ttaaacttag
    31441 agtcatgatg aatcctcatg atcttccaat atatcaagaa aaatctcgaa tcttagaagc
    31501 tctagcaaag aaccaggtta ttgtcgtaga gagtcccact ggttcgggta agacaacaca
    31561 gctacctatt atactctatg aggctggata tgcagcagat ggcacaatag gcataaccca
    31621 accacggcga attgcgactt tgtcagtaag cgattttatt gcccgtcaac tcaataccac
    31681 tgtgccggga atcgttggat acaagatgcg ttttgaagat gtgacagccc cgaatacagc
    31741 cataaagata atgacagatg gtattctctt gcaggaaatg aaatttgacc cgtatctttc
    31801 ttcttacagc ataataatgg tcgatgaagc ccacgaacga agcctgaata tcgattttat
    31861 actagggctt cttaagaaag tcatagatgc tagagatgat tttagggtaa tcgtatcttc
    31921 agccacaata aatgcggaag ttttttccga atattttgga gaatgcccag tcgtaagaat
    31981 cgatgctcct atgtttccgg tcaggatcat ttatgatgca attcggcctg tatcagaaac
    32041 aggatcaaga tatattccca acgccaaaga aagcaatgag ttatattatc gatcttcgga
    32101 tgaggctctc tatgaaaaga tcctttccat catagataga gtactgagag gggaaacaga
    32161 tgccgaatct ggagatattc ttattttcct tcctggagaa aagacaatca aggagtgcct
    32221 acaggttctg atgacaagcc gctgggcttc taagctcgat tgtattcctc tatatgcacg
    32281 cctaggcaag gatgaacagg aaagagtctt tgagagtcct ccaccaggta aggtcaaggt
    32341 ggtaatagct accaacattg cagaaacgag cgtgaccatt gatggaataa ctgttgttgt
    32401 cgactctgga ttggccaaaa tcaattatta taatcctaaa acctttactg caagcctaat
    32461 tgagactcct atttcaaaag cttcctctaa tcagcgcaaa ggcagagcag ggcgcacacg
    32521 tcccggaatc tgctaccgtc tgtattctct taaagatttt gaatcgcgtc ccctctattc
    32581 cacagaagag atcagaagga cggatctatc ggaggttctg ctgcgaatgg ctgaactggg
    32641 cataactgat tttgagcatt tcgacttcat aacaaagccg ccaaagagcg caattcgagg
    32701 agccatcgaa gcactgaaca tgctcgatgc actaaataca gacagatctc ttacacatat
    32761 tggcgagatg atgtgtgcct ttccacttct gccaaagcta tcgcggatga ttgttgaggc
    32821 tatccttaag tatccaggtg ttttgagcca gacgcttatt gcagcggctt ttctatcgac
    32881 tacaagccca tatattcttc ctccaggtga agaaacagag gcgcgatctg ctcatcatca
    32941 ctttttagat gctatgggcg actttgtctc atatctaaag ctctatgacg ctttctcttc
    33001 tgctcgggac aagacaaaat tctgtgagcg caactattta gatgagcgca cgcttcgaga
    33061 aatccttaga atcaaggagc agcttgacct aattgtatct gatatgggca ttcctattgg
    33121 atcaggtggt tctattaacg attatctatg tgccgttagt cgaggactta ttcaatttgt
    33181 atgtgcacga caaggaagag gcatatttag atctctcacc gcagaaaaaa tccatattca
    33241 tccgggctcg gttatgtttc gacaggatgc agattttata gttgcaggcg agattgtccg
    33301 tactaccaga atgtatgcga tgtctgtttc tccactgtca aggtctcagc tatataaaat
    33361 atcgccggaa ctagcagaac agatcattgc attaaagcag aaggaacctg tctcaatatc
    33421 aaaaggcaga gaaaacagag agccgaaaga aggcatctcc gacagagggc atttgccgaa
    33481 agagaccaaa tcaggccatg ttttcaaaaa gcatgatcta gaccgctagt tcttcctgta
    33541 aaagagctcg aaatgttgat tcccagcctc ttttgcaagg ttttcaaatc tagtttccgg
    33601 acgccatggt tgaaaaggtg cgaagtcctc atattggttt tctagcagtg agcaggcgtc
    33661 aagcgaggct ttagcaaagc tagcatattc ctctatatca gttacacaat agagatagcc
    33721 tccaggcata agtttttgag ccatgagaaa gacgattgga tattgcatga gcctgcgttt
    33781 gtggtgtcgt ttttttggcc agggatccgg ataaaaaaca tggatgccgg caaggcttct
    33841 aggcgctatc atgtgcctca acacagcgat agcatcttgc tgcaatatgc gcacattctc
    33901 tatggcatgg agttcgaggt ccattattag ccttcctacg ccggacgcat aaacctcaat
    33961 tccaagatag ttgaattcag gtctttcttg agcaattcgc catgtcgctt gtccggatcc
    34021 aaaaccaatc tccaagatta ttggtctatt ctgaggaaat aattcttcca gaatcagtaa
    34081 ttcttccgaa aatggaatta tgtatttggg tcctaataac tttatcgctc tgcgttgagc
    34141 ttcggtaatt cttccggatc ttttcacaaa ggaacgaatg cttccgtcca gaagcccaat
    34201 ttcttggctc atcgattggg tttgctcagg ttttctgtct tgtccttctt ccatttcagg
    34261 tttggacatt caggaagttc ctaataaatt tgcagaggct gtcgaaatcc agacactcta
    34321 tgttccttct ctccgctaga atcctgatat tataggggcc ttttgccgaa ttggtgaaga
    34381 gagcaagagg aatgtgtttt cgctcggcat aggtatactg ggcaagggtc tttttctcct
    34441 caggaaatac ttcacaggaa aaaccggcat ctctaagacg agtcgctagc gcatgaagct
    34501 ctgatgtcgc gactttacct acgttccctt tttcctgatt cattatcagt atttgggtcg
    34561 atattattga cttgtctatt ttaccgattg tctctaaagc tgcgataagt ctatccaaac
    34621 cgattgaagc tccaactccc ggcattcttc tctcggtata taggcttgcg agttcgtcat
    34681 atctgcctcc tgagcatacg gagccaatct caggcagagc tcctagcgat gtctctacaa
    34741 ccattcctgt ataataatcc agccctcgag tgatgcttgg atcaagcata atccgattct
    34801 caaagcctgc tgcagccaca tattccatta tttccagcaa gcgatccctt gccgattgcg
    34861 cctgaaaatc atttttatcg caaaaaccaa tcatcttttc aagagttgct tcaaagctct
    34921 cttctttttt gataaaagag agaatatcgt ctgccaattg cagacccagc aacttcgaga
    34981 gcttttcgaa ggcattctca aaacctattt tttctagctt gtctacggtc ctgagaattt
    35041 ccgtagatct ggctgaagcg ccaatatgtt ccaagaatct attaaaaagt gcgcggtgat
    35101 taatgcggat cgagaattct ccgacgtcga gtcttagcat cgctttggct gctgtaagca
    35161 gaatatctgc atccgcagag cctgaatcag tacccacgat atcaaaatcg cactgcatga
    35221 attcccgata tcttccgcgc tgagtattct ctccgcgcca tacttttgct atgtgatatc
    35281 tcctaaaggg caaatataac tcgtcgacat gttctgccat aaatcgtgcg aaaggcactg
    35341 tcaaatcgaa acgcatggct acgtctcgat cgccatgatc aaggaagcga tatacttgct
    35401 tgtctgtttc tccgcctcct tttccaagca agatgtcagc atactcgagg acgggagtgt
    35461 ctatgggtac aaaaccaaat aatgcaaaga cttgctcaag ttctcttacc agagaagagc
    35521 gagttatctc tgcatcaggt aaaaaatctc gaaaaccttt tagcactctc ggcttaataa
    35581 cagtcattca ttagctccat caatatagaa ttaagcagta ttgcaaagat aagttgagta
    35641 attatgagaa taatcagcaa tggatgcaag agggctagtt atacttagaa gagacatatg
    35701 caaatggtct atgcttgtcc ttcgcattca tttgtgatat atagataagg ccctctttat
    35761 ccggttctat cagccggatc atccgcactc gtatgagtgc atatgtccac agggaggatt
    35821 gaatgagaca gtatgaacta gtcactgtgc tgagctcgga agaagaagaa ttcaaggccg
    35881 gcaaacaggc aatcgccgat atgctttcgc agtataaggc aattgatgta aaagaagaag
    35941 atatgggaga caagcctctc gcatatccaa tcaagaaaaa gacacgcgct cactatatgc
    36001 tctatcgaat gagttttgat cctgcttcca ttgtttcgct cgagcgcgca ataaagctaa
    36061 accctttggt attgaaacac ctggtcgtaa aggttgaaga ataaggccaa gtcccacaag
    36121 gactaaagga gtatattgtg agtgatatca atgtagtagt gttggttggg cgtcttaccc
    36181 gcgacagtga attgaaatac acgccgtctg ggcttccaat atgccgtttt tccattgcag
    36241 tgaatcgctc taagaagcag gatgaccaat ggattgatga gccgcattat tttgatatcg
    36301 agttttatgg gaaaagcgca gaagggctaa gcaaatacct tttcaaaggc cgacaggtcg
    36361 ccgttcaggg agaacttcgc caagataggt gggaaaaaga tggccaacag cgctcaaaag
    36421 tagttattgt cgctggaact attcgaccat tgggagctcc gtctcaaaat agtggcgaaa
    36481 tgggtggcag aagatatgca gcagataata ttccatcaaa gattgattct gccaataatc
    36541 agatgcctat tcctccgaca atcgacgagt ttactgatga tattccattt tgataggaga
    36601 ttagtatgga tgagatgaga gatatcgatg aatctagagc ttctaatgta gaagcggaac
    36661 aacaagaact gcacagagat caggatgacc ggaatggagt tcgcaaagga aagggcttct
    36721 ttcgtaaaaa agtctgtcgc ttctgcactc agaatgtaaa gatagattat aaagacgcag
    36781 agcttcttcg gcgttatacg actgaacgag gcaagattct tcctaggcgc atcactggta
    36841 cttgctcaaa gcaccagcga atgctggcag taaatatcaa gagggcaagg gctcttgggc
    36901 ttcttcccta tgttataaaa tagcccagcg tttggaatct ggtccaaaga gcgagcgaca
    36961 ttgatggaag tatctcagag cataaagtca gaatcgtcta gcaaatattc atggcgtgtc
    37021 tttattccat atggacttct gtctggattt ctctatgcta gcggttttct tgcgtttgca
    37081 tttttgatcc ctattcaata tgtgtttcgt aaggagaaca agagggaagg tttgctctcc
    37141 attctagtat cactctcgat agtggcggca ataagcacaa ttgaactttc taggatgggc
    37201 tggaaagagt tacccatatt tcttcagact ctattgcttc cagcattgct tctagcaaca
    37261 ataggagcaa tgaatctatt aaagagcgat gcctgggtaa aaatcatagc tgctacaata
    37321 gttgtctctg tgctatttgc ttatcttttc agtattagta taggtacacc agaagcacgg
    37381 agatcgatag cagatatgat agcccagctt ttgaactcat caggcattca ggatttggac
    37441 gcatcggcta tcgaacagaa ttacgttatt cccgcaatat cgatcattat gagatctttc
    37501 ggagtagcat cgtggcttct gcttgcaggt agctggcata taggcaatag tcttgcatct
    37561 gctagaattt ggaagcaaaa aggagaagca aagaagaatg catttgtatt cagcgtgcct
    37621 tcatggcttc tctggccaag cattttgggt tggcttgctc taatctgcgt tctttatgga
    37681 aatgagagag gagtctttgc cataatcgcc tggaatgtct ctctggccgc tgcttgttgg
    37741 tatgcattac aaggctttag cctgatttcg tatttttttc gattgagaaa tatgcagcgc
    37801 ctagctggcc tattattgat tttgctcatg atactcatgt tttacgaagc caaaatcagg
    37861 attgcagctg caatcctggc cccaatgctc ggtgtatcgg aagtttggct gcaatatcga
    37921 atgcataaag gagcttgaac atgaaagtta tattaaatca agacataccc aatttgggtg
    37981 aaattggaga cgtcaaggaa gttgcgccag gatatgcacg caattatctt ctgccaaaaa
    38041 aaatggtcat ggtctacaat gaaaagaccg aagcattgtt tttgaagcga caagcggaga
    38101 ttcaaaccat taaagagcag aaaaggcttt cttctcgcac tttaaaagag agaatcgagg
    38161 cagaacctct cgttatctcg atgcctgcgg gaaagaatgg gaaactttac ggtgcggtta
    38221 cgagccacac aattgcagaa gaacttttga agaaaaatat ggaagtcgat agaaagagaa
    38281 tcgaagtgcc agatcgttcg ataaagtctg tcggcaacta taagatcgtg attaagcttt
    38341 atgaaaaaga cgaagctata cttcattgta atatagaggc tcaagcatca gcgagtgcag
    38401 aggagaagaa accttccgaa gaagcaggca agaagcgcag aacccgcagg caacgcacag
    38461 aatctgagcc agcaacacct gaagaacaag ctgcagcatt tgaggcagcc gtgaatcgcg
    38521 gtcagttgag ctgatctagt atttaaccct ctaaaagggt agggtacatc tagaggattt
    38581 ttagatggca ggtccatcgc ttaaagacac attaccgctt tataatggtg acgctgagca
    38641 ggcatgctta ggcgctcttc ttctagaccc cgagtcgata aacaatgtta taagatacct
    38701 gcgtcctgac agcttctatg attcagccaa ccaggaagta tttgaagctc ttctcgctat
    38761 gcacgaaaaa gggcagaagc cagacctgat tacactttca gaggagctgc gaagcagcgg
    38821 caatcttgaa aggatcggtg gaagtgcata tattgcaagt ctggcttcat ttactccttc
    38881 tgctgcaaat atcgaatact atgcaaaaat tgtgcaagaa atggccacaa gacggcgact
    38941 agtgcaccta tccgctgaaa ccgcagccat ggcgcatgaa gaaaccaagg atatcgatgc
    39001 gattctcgat gaaatacagg ctaagatatt cgagatatcg cagaatcgaa ggactgctga
    39061 gtatcactct gccaaagaga tcgtaacaga aacgatgctg ctaatagaga aactcagtac
    39121 ggacccagct gcatttaccg gtataccttc cggcctctcc gatctcgatg cattaacgaa
    39181 tggtcttcag aagtctgaat tcatagtcat aggggctagg ccttcggtgg gaaagactgc
    39241 cctggctctt acaatggcag cgcatgcctc aatcaatcta aagattccag ttgcattttt
    39301 ttctttggag atgtcggaat cagcaattat gttgaggctt attgcttctg aagcaaggat
    39361 tcctgccgat cgcataagga caggtcgaat aaagaccacc gattatgata gccttatgga
    39421 tgcagcctca agaatatatg aagctccact ttacatagta gatctgccaa acatgaaact
    39481 ccttgagctc aggaccatgg cgcgcaggct tgtactagaa cgaggggtaa gagcaatatt
    39541 cgtagattat cttacgctaa taacccacga aaacgccgat ttgccccggt gggaacagat
    39601 agcggctatc tcgcgatcgc tcaaggctct cgccagagaa ctagatattc caatcgtggc
    39661 tctttcgcag ctcaagcgcg aagcagaggg caaacagccg actcttgctg atttgcgaga
    39721 atccgggtcg atcgaacaag atgccgatct tataatcttc cttcatagag atagagaaac
    39781 ttccaagaca tatgactcac agaccgatca aatcgaaacc gatatcattg ttgccaagca
    39841 gcgcaatggg ccaacaggta aggctccggt ttggttcaag agtagctatg cgaaatttgt
    39901 gaacatggag cgtagagatc gcacataatc ttcatgagtt gcatccctat ttacctaaaa
    39961 agaagaaaat atcactcata gttgctacaa ggaatagcaa gagaatagaa gcgaccccaa
    40021 tgaattgaaa tctataaagg gacttggcat taattggacg ccttctgata agctctatca
    40081 aataaataag gacacgacca ccatctaaag caggaattgg aagaagattc ataatggcaa
    40141 gcgctatcga tatgaaagct aggaaatcga gaattggtac cagcccggcg aggccttcag
    40201 cttttatctg ttcggatgca gcggtaccaa ttagataggt caacctggct ggcccggaaa
    40261 tggctttgaa gacatttaca ccgcgaaaaa gcatgcctat actctttatt tccaatgaaa
    40321 ttgtagaaaa ggtctcttcc acccccatcg aaatagcctc tgggagcgac ttagcttttt
    40381 ctgttcgcaa gataccttca aatctaatgc ccgcttcttc aagcgatttc gcgacaatta
    40441 tcattgagag agtctgatct ccgcgcttta ctctaagact cacagaacca tttgcattgt
    40501 tgaatacttg cataagatct acggtgttcg aaatgggtct tccatccgct tccataatga
    40561 tgtcacctgg acgaagatcc gcaattgccg cgggactctt ctcttgcact gattctataa
    40621 taggatcaat ccaggcatat acgccaatta cgcctatacc cgaatttgga tccagtaggg
    40681 gagttatgac gagcgaatgt tttttgtcat ttcttaagac ctcaatggac atggtttttc
    40741 caggattgct ggcaatgatc tcctggagat cgctataatc ccttattgtt ttgccatcga
    40801 tcgatataat cttatcgcct gtcttaaggc ctgcaatatc cgcaggattg ggcattccgc
    40861 tttgaagagt ggacccaatt tcgctcgaaa gaatgatcct atttggtgcc gttacaatgg
    40921 tagtccctat agatgctacc aatatgaaga taaatgcagc aaaaagtacg ctgaagagag
    40981 gcccggcaga agcaatgaag atgcgcttta atggagtagc cgaaaaaaaa ctaccttttt
    41041 ccgaagggat atattggaga ttttgatcca ccgctgcttg aaatccctct tctcccttta
    41101 acttgcaata tcctcctatg ggaaaagcac aaagcttcca taccgtcttt ttgcccttgt
    41161 gggagattat tgatggtccc caacctacag agaaagcctc tacttctacg ccagacaagc
    41221 gagcaaagac aaagtggcca aattcatgaa atatcacgac tatggaaagg ccaataaggc
    41281 ctagaataat actgatcatc cacaactctc cgagagatga gcggcaattc tgcgcgcttt
    41341 gatatctata tcgaatatat cctcgaatga ttcgataggc aaattccaat ctcgatcgag
    41401 tacctttgcg accaggttcg atatatcggt aaatctgatt cttccattat cgaagcattc
    41461 taccgctatt tcattggccg cattgaacac aatgcattga gcatctccct cgtcgatagc
    41521 ctggcgtgca atccagagca taggatatcg ttcttgctta ggttcataga aatcgaggct
    41581 tatgtctgct aaatctgcaa acccgaagga agtctctaca atctcaggcc aatacagcgc
    41641 catttgtatc ggcagacgca tatcaggttt tgagatttgg gcgtagagaa ccccctcgat
    41701 agtacgaacc attgcatgga catagctctg cggatgaata agcaccttga ttcgctcttg
    41761 aggcatatca aaaagcctgg aagcctcgat tagttccagc cctttattag ccattgttgc
    41821 agagtcaatg ctgatctttc gtcccatttg ccatacaggg tgcttgcaag cctcgtctgc
    41881 cgtaacagca tcgagcaatt caagaggaag agcatagaat ggtccgcctg atgcggttat
    41941 tatgagttcg gagacaaggt ctctgccaaa tctctggact atttggaaaa tcccagcatg
    42001 ctcagaatct actggtatta tttggcattg atttttttga gcaagattcc ttagaagtct
    42061 atagcccatg acgatggatt ccttatttgc cagggctagg tctcttttgt tctgtaatgc
    42121 tagaatcgac gccgatagac cagcagctcc agcgattccg tttaggacta tctctgcttc
    42181 ggtttccaaa agaaggttcg atattgcgtc gacgccattc caatccatct cttctggagc
    42241 aagagatgga ttgccagtcc aagctccttt ggcttgagga aattgccttt tgagcttttt
    42301 ggcgaggatg cgattgctat ggatggaaaa accaacaagc tcgagcttgt tgcaggaatc
    42361 cgcgctccgt tgaataacat cgagagcctg tcttccaata gagccagaag ctccgagtac
    42421 gataacacgc ttcagctttt acctccgata cccttgcttc tagagattga acaaatgaaa
    42481 agtatacgaa aatgcaataa aaaagggtgc actaaaatat agactatcaa atgaatccag
    42541 aattcctccc ctgccaggta cgatagagcc tgaatcctta attcctgccg atctcttgag
    42601 agcgctttca aacaggtctc ccacaataac aaagaatgcc attcccaatc caagagaaag
    42661 gcttagtaat atatatgcaa ttcccgaagg cttccaagcg ccggcaatag gtcccagtcc
    42721 aataaaggca gcgactatcg atccaatcgt acctcctata aaacccgaaa tgctcttgtt
    42781 tggtgataca tctagtagat tccttttcct agcaaggtat tttccaaaaa accatgcaag
    42841 gctgtcgttt ccaaatgtaa gcaaagcaaa gctgattatc gtattctgcg aattaggaaa
    42901 tccggaaaca atgaatatga gaaatcccgg aagaagcgct gtataacaat ggacaagcgc
    42961 gattgatgct gcttgcttga taagatctgg gaaagaattc tttttttgga atacaagcgg
    43021 gattaacaga agcattaagc aaaatgacca ggaaataaat aagacaaatg cggtctccag
    43081 gagagatcca tatagtatac acgatataaa tgtcaagact gatggaatta atccaatgaa
    43141 agttgctaag tctcctgtgt ccggcaaact ggctctggtc agcatgtttc gcaattcttt
    43201 ggatgaaagg aattgcacaa tgaggagaat tcccgaaata actatataat tcgcaaattg
    43261 aagaaaaaaa agcgaaagga ttaataaagg aaaacctaca aaaaacagga ggagtcttgc
    43321 cctcatattc tcattcaccg tgcatctccg aatcgccttt cgcggtgcac atagtcgata
    43381 agagctgcgt gaagatcctc cggggtaaaa tcaggccaat attttggcga aaagtatagt
    43441 tcgctatagg cagattgcca taatagaaaa ttggataacc tgatctcacc acctgttctg
    43501 atgatgagat cgggatcagg tatttccgga tgatcgaggt atcgcataat tttcaattca
    43561 tctattggat tttcatcatc attttcttga cttgccagaa ttcgctgcat ggctcggaca
    43621 atttcgtctc tgcctccgta gtttattgcc aagttgactg tcaatgagct gtactgagag
    43681 gtattctcaa tcgccttatc gatttctgaa agtacatcat gcggtagtcc atttcgttcg
    43741 cctgaatgga ctatgcgtat ctcgtttttt ctgtagaaat tcaactcctt tgtgatatga
    43801 gctcgaataa gagacataag aaatccgact tcctgttctg tacgcttcca attttctgtg
    43861 gaaaaaacga atagcgagag aaattcaatt ttttctttcc gagaagccag cactatacgc
    43921 tttgctgtct ttaatccttc cttgtgccct tctgttcgag caaggcctct ctgtcttgcc
    43981 catcgaccat taccatccat aatgatcgca acatgagaag gcatgcggtt ctctttcatt
    44041 ttgaagaatc cttagatttc cataatttct ctttctttta tttcgagaat tctggctatt
    44101 tgggaaatgt aatcatctgt catcttttgg agctctgctt cttctttttt aaggacgtct
    44161 tctgcaacac ctcctgcgcc ttgctgcttt ttaagctctt ccagagcatc gcgtcttata
    44221 tttctgattg ccacgcgtgc ttgttcggcg atacttcgag ccgttttaac aagttctttt
    44281 cttctctgct cagttagagg agggatagct atcctaagca cttttccgtc attagaggga
    44341 tttaagccta gatctgactt gaggattgcc ttttcgattt cgctgaataa agagcgatcc
    44401 catggctgaa taacgatgag acgagcttcg ggaacagaaa ttgtcgcaac ctgggataga
    44461 ggcgtttttt gaccatagta gtcaactcta atcttgtcga gaagaacagg ggatgctctg
    44521 cttgtacgta tgctattcag ctcatcttga aatgcagcga tagtcttctt catcttttct
    44581 tcatgcgcag ctttgttcga ttccataaat ccttccttga catgctgttt tgcaggtgca
    44641 gagaggcatt ctgaatagaa agcccgtctt tacccgatat tagcagagca tattcaatct
    44701 tgtccgactt ttgcaaaata ataatctacg atttcgagct gaaaaccagt ctctttagaa
    44761 atctgcgcta gaatagaggc gacagatgac ttctcgtctc gcacaaagcc ctggtttaga
    44821 aggcaaacat ctgcaagaag cttctttagc tttccagcca aaatgccctc aaggactttt
    44881 tctggtttgc tcttcagctt ttcgtcgagc tcgagctgtt tttggaagat ttctttctgt
    44941 tcggtgatcc actcagccga gacttttgat tcatcgagga acattgggtt gaaggctgcc
    45001 acatgtaaag ctatatcatg tataaaggca gcgatcttct catgcttgaa agcctcaggg
    45061 tcgttcgctc taacttttac gacaacccca attctgcctt cgccatgcag ataggggtgc
    45121 agaatttcgt tttcttcggc ataaaaatga acaacgcgtc tcagcgcaat gttttccttt
    45181 ataagcgaag caatttcctt tacaatggcc tctaggctct cgtttgtact ggatgccttc
    45241 tgtgacagag cgctttctgc gattctagtg cctgccgcca caaaatcctc atttctagcc
    45301 acaaaatccg tctcgcaagc cagctctatc agttctaatt ccttatcatt ttgagctatg
    45361 aagatacgcc cttcatttgt tgcacgacct gctcgtttct cgataccggc aattccccat
    45421 tcgcgcaata atttttccgc tgcagagaaa tcgccattag cttcaattag tgcctttttg
    45481 caatccatca tgcctgcgcc ggttttttcg cgcagagcct ttacttcatt cgctttgatt
    45541 tccacgatct attctcctca atgttctcaa tcttcatcat aaagacgatc ttcatcaatt
    45601 atgggttgtt cgacttcctg ctgttcatcc gcttctttgt tactctgact tggggtttcg
    45661 gcataattct ctacatctat ctcgacctca tcttcctcta gtttttgagg aactatcagt
    45721 tcttgctcct cttcagaagg cagagtctcg attatcttca agccttcgga cgcgccggcc
    45781 tcgattacag catttgctat gatctgagtg aaaaggctga tagatcgaat tgcgtcatca
    45841 ttgcctggaa tgggataggt aatgccttcc ggattacaat tggtgtcgac gattgctacg
    45901 atagggatac cgaggcgttg agcctcagca accgcgattg cttccttgcg cgtatccaca
    45961 acaaaaagga tgcccggaag gctcgtcatc tcctttattc ctccgaggtt tttctcaagc
    46021 ttggtctttt cctttagaag acttgcgatt tcttttttgg taagactgct gaaagttccg
    46081 tccacttcca tgcgctctat ctttttgagc ctttgaacag attttcttat ggtggaaaag
    46141 tttgttaaca taccacccag ccaacgattg tttacataga attgctcgca gcgctcggcc
    46201 tctttttgta ttgcggcttg ggcttgtttc tttgttccca cgaagagcac agatttcccg
    46261 gaactggcta ccttctgcac tgcgtcatag gcttctttga tggcttgaat agttttttgc
    46321 agatctatga tatggatacc gtttcgctcc gcgaatatgt attttttcat tcgcggatcc
    46381 caacgcttga cctggtgccc aaagtgcaca ccggattcaa gcagattttt catggtcact
    46441 actgccacgt agacctcccc ttaccggaat ggattccggt tcccttcctt atatctcgtc
    46501 ctgctctctc ggaaagtcag gcggaaaatg cccagcactc aaaaggctgg atttatcggt
    46561 gatttgaatc tcttcttgct aagaatccaa gtatcccgac gcatggagaa tgccatataa
    46621 ttcatgaatt ggcaagccca ttacgcccga ggatgaacct cttatttctt ctatgaagca
    46681 tgatccgatt tcttgtattc ggtatgctcc tgcggctccg cgccattcgc cgaagctaag
    46741 gtaccatgag acttcttcag gtgtcatggg tgcaaactgg acttcagaga tagagagagc
    46801 ggggtagcat cgatcgctta ccatgtcgag caggcaaagt ccactaaata cagtctgtcg
    46861 tttgcctgct tccatcgaaa gcatttcgaa agcctcgagt tcgttagccg gttttccgag
    46921 cgtaatccaa cgcccggata tcttaaaggc cacaagtgta tcggcaccaa gcacatatcg
    46981 aggttctgcg ttgctttttt gatctagagc atttttatct aaaggcttct tggcagacga
    47041 cgatataact acctttttgc ttcgattttt ctcggaccaa agagctttgc catgcttggc
    47101 ttttgttgct gcaagagcta caacacgttc tgcgggatct aggttgtcaa atattgtctc
    47161 atcgatggaa ggtagaactt tgataaaagg tattctgatc gattcaagaa tagaagccct
    47221 tcgcgaggat tcacttgcaa gcagaatagg ttctccatac atgacaaaaa gcaatctaat
    47281 gagagatgga tgctttgaca ag
//
LOCUS       DAOOWY010000004        47080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_821_length_47080_cov_12.557661, whole genome shotgun sequence.
ACCESSION   DAOOWY010000004 DAOOWY010000000
VERSION     DAOOWY010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 47080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47080
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_821_length_47080_cov_12.557661"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PK105_00680"
     CDS             <1..759
                     /locus_tag="PK105_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOU60246.1"
                     /translation="VIMPPGSNPHSFEPSPRQLAELGNAKLWFSINVDFEYSLKPKLR
                     AMFPKLQIIDVTKDVQFRKLRPGEKEHDQDAVDKYAENSDPALVNLDQHTWLGIEQAK
                     TEIMVIQDTLIAIDPENRELYKTNCQDYLKDIDAVYSALKTKLAPLSGARVFVYHPAF
                     GYFLDMFDIEQVAVELGGKEPTQRELYALIELAKKEKACAIFTQVQFSESAARAIASA
                     IGAVVVPIDPLASDWLENLSRIGQALSMSIGKRE"
     gene            761..1651
                     /locus_tag="PK105_00685"
     CDS             761..1651
                     /locus_tag="PK105_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60247.1"
                     /translation="MNQEIKYRIASLRQRTGSLNSIEGKEHGASIEPTLPHPPDNVAI
                     WCHDLGFCYREAEVFRNVSFHVHSGEFVVLTGKNGAGKSTLLRLIVGLLEPQQGQVLV
                     FGVSPRRRIESIGYVPQHTNFDQAFPIRVEDVVGMGRLSGLSYISKDQKKVDIEWAME
                     QAEVTDLRARPFSALSGGQRRRVLVARALVGHPRLLVMDEPTASMDIASENKFFEVLA
                     RIKRSTTVLIATHDSNFVSGLADTVLCVGQDSEHPHSVHRHAMEKAEDLPKDIYGSNM
                     LRVRHDIELPDNACCEGKDQ"
     gene            1648..2505
                     /locus_tag="PK105_00690"
     CDS             1648..2505
                     /locus_tag="PK105_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOU60248.1"
                     /translation="MSSFFQAVINPSIPFVRNALIGSVLAAFLFGILGSLVTVRRIAG
                     LAGAISHTVLGGIGLALFLVSRGAPEWISPMLGALVFAILAAITISIVSIKAKQREDT
                     VINALWAIGMSLGVIFMAKTPGYADPMSYLFGNILLVTQKNLYMLGALNILVLLLVWR
                     YYRQIEAASFDEEFAATKGLPVMVIYLVLLLVIAVAVVMLQTFVGIVMVIAMLTLPAG
                     TAGFFSRNLAKMMINSSILALVFSVLGLILSWVFDLPAGALIVLIAGMVYIGISVVRM
                     LQGRRSSTS"
     gene            2600..2983
                     /locus_tag="PK105_00695"
     CDS             2600..2983
                     /locus_tag="PK105_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOU60249.1"
                     /translation="MTKARNHVLESIIKAAEPLSARQLCDKYKSEHDPATIYRSLHFL
                     EEKGRIDSFILYCNAHGTERYYVLHRGEHRHWFHCERCHRFTDLGECRFAEIVSQISA
                     EKGLLITSHSFYAIGICSECRQQGS"
     gene            3013..4008
                     /locus_tag="PK105_00700"
     CDS             3013..4008
                     /locus_tag="PK105_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU60250.1"
                     /translation="MNEEKELSTYLELLAHLKEKDLAKQGLMALEGRIVIEKALEAGI
                     VPRILVCTEAEKDYWHERTKEENSMVGAHTPRSRELDFPIYTTSHEALCSLVDFKFHR
                     GAIAMADVPELELFDMEHNSQLHQFHRAHCTHAVWSPTIAGVRAPLGRRRPFYLCLWN
                     VTDPSNLGALIRTAAGFGVDGVLIGPGCANPFYRKAIRASMGNVFVLPLWSVDVHGLE
                     KLKQNGAVLVAAALDENSMALEELVKELEKLESSFGASEDEYIKDESSVVGCAQNSPI
                     ILLLGNEGYGLPAEVLGLSSIHVRIPMERGVDSLNVAVAGGILMYEIIRAPQSLC"
     gene            complement(4083..>7287)
                     /locus_tag="PK105_00705"
     CDS             complement(4083..>7287)
                     /locus_tag="PK105_00705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU60251.1"
                     /translation="AQKKLIARDEVELLPDPLPIDLVRPWKTSEKMEMLEFPGIRAYD
                     KTILGLLPPPPPLAPYDLSSALAGFYRDIDQHWDRILELILPEDELEERARTFPQSRI
                     EIHLFRGEKSSQSKKPFITAIRASGKKEKLSALEAVRLCLAIDTSEGSLGYRFLREFS
                     RAMRSIIAAKAFVPDIAMYDAVKKGKRAGGTFMRVVWKPALFSADIRSLFALIEPLVP
                     VPVFIDESAAHGEPVSNASTLKVVKVSVAPREKSRVPSPHATLLLLASDILSDIVTEL
                     RYLPSGVKDISHPVTATLFRNEDANVSSPLHRSIPKAMRSWLSVFDLANSGFQYELVV
                     KNCGEIDTSNWGDEDDSIPDGMPRYELSATVRQSSTATNKVKTAQEERIPLHEAAQRF
                     GISALTFPALLSTFVPELAQLGSKASVILDEIALSRFVIESAALLGNLGVTIALPKEL
                     CNLIKPRAVLTAKKGKKSGNLVSFLTLDSLLSFEWKVSIGDRLISAAEFERMVKAGTA
                     LVRFRQGFLRLEASEAASMLERIKKGPKPDAFDVLQEYLAGTAEFDQALQEKLAAMLP
                     RTIDGAAQAKVGTSDVMPHDVMSDGAMPDSDIPLPASLNASLRPYQARGFRWIATTME
                     RGFGCILADDMGLGKTVQAIACILHLKETKSLTNGALICAPATLITNWERELEKFAPS
                     LSVSTYYGAGRHHKSADILLTSYETFLRDKEKFKDKKWDIAILDEAHLIKNPEAKRSK
                     AVKEIVASDRLALSGTPVENHLGELWSIFDFVIPGYLHTLEHFRSNYRKPIEVDKSKE
                     VADRLRRVTAPFLLRRLKTDRSIIADLPDKIVVNEYATLTAEQAALYESTVRDCMKKI
                     ADSEGIARRGLILALITGLKQICNHPRNYDKDSAPSAERSGKTRLLLSLLESIAEAGE
                     KVLVFSQYVEMLDILRQIIAQDRGVEPFMLHGKMTRTKRDAAIEGFRNASGPAILLIS
                     LKAGGLGLNLTEATRVIHFDLWFNPAVENQATDRAFRIGQRKNVFVHRLITKDSFEEK
                     IDAILTTKRDLAEMAISAGETWITELDNKALADLVKR"
     assembly_gap    7288..7387
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7388..7891)
                     /locus_tag="PK105_00710"
     CDS             complement(<7388..7891)
                     /locus_tag="PK105_00710"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOU60252.1"
                     /translation="MPRKYEFGLTPWGAYFIRAMESLADQARLKRGRSYAANGNVFRL
                     SIENGVVSAMVEGNYKPWYDVRIVFKPLNQSERTALFRLINDDPMLVGRIAIGELPAE
                     LIDRLRRANVRLLPERWNDMRRSCTCPDYGDPCKHMAAVYYVLAQEIDRDPSALFRLR
                     GVDIFSEF"
     gene            8174..8413
                     /locus_tag="PK105_00715"
     CDS             8174..8413
                     /locus_tag="PK105_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60253.1"
                     /translation="MVPLRQNTLGYNRTRLRSVDKSGFDGEFEFGGRSTWYCHTHEDT
                     HQSILVYLDGALRHRIIYKVLETEIIVTILAKRRK"
     gene            8428..9921
                     /gene="glpK"
                     /locus_tag="PK105_00720"
     CDS             8428..9921
                     /gene="glpK"
                     /locus_tag="PK105_00720"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOU60254.1"
                     /translation="MDRNSTYILALDQGTTTSRAILFNHDGIIISVKQIPFKQIFPKP
                     GWVEHDPEEIWRTQLKAARNAIEAAQIEPTQIAAIGITNQRETTIVWERSTGKPVYNA
                     IVWQCRRSSSICDEFKAQGASDLIRRKTGLVLDPYFSGTKLTWLFRNYPEIKARAERG
                     ELMFGTVDSWLIYNLTEVHATDPSNASRTMLFDINERRWDEELLSLLEVPANILPEVR
                     PSSALFGVTKKDIFGAEIPVAGVAGDQQAALFGHACFEPGDVKNTYGTGCFTLMNTGP
                     FPVESKHNLLATVAWDLGKGYTYALEGSVFIAGAVIQWLRDQMGLLTDSAESERFARM
                     VEDSQGVYIVPAFVGLGAPYWDSEVRGTVVGLTRGTSKAHFIRAALESIAYQSMDLFE
                     AMEKDSGKRLSCIKADGGASQNSFLMQFQADITGTRVVLPEVSETTALGAAYLAGLAI
                     GYWNGLDDVRKNWRMRREFSPRMAEESRVGLVVGWRKAVATARNFKQ"
     gene            10002..10463
                     /locus_tag="PK105_00725"
     CDS             10002..10463
                     /locus_tag="PK105_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOU60255.1"
                     /translation="MERADEAVSMHVAGSACSQAVFTVFADELGLEPSVAHRISGGFG
                     GGIGRMGLTCGAVTGGIMALSLALGATDSADQDSKLALYQVVAEYMERLKAFKGSIEC
                     RVLLEGVDLWTEEGRDMVKARQLSEIVCNSMIHKAVEEAERILNEKDRFGV"
     gene            10465..11298
                     /locus_tag="PK105_00730"
     CDS             10465..11298
                     /locus_tag="PK105_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOU60256.1"
                     /translation="MQYERFEGSWFLKSNWHLVKTGQSDQSKGVPVPPQEEPPAPEDC
                     VINLPPPDSILRAFEESREQKRAAASFADTRTSEKPFWGVPERAAVLYHLLNNRRSRR
                     KYTMEPLTIEELSYLLWAIEGVKENRGKFSFRTTPSGGARHPLDIYVFAHKIKGLNVG
                     LYRYLPVEHELVLERQGDDSIALNEALNGQFWNAACVIMWAAVPYRSEWRYGRAAYKL
                     VALDTGHSCQNLYLACESLGLGTCAIGAYDQEKLDAYLGLDGEEMFAIYTAPVGHVAQ
                     P"
     gene            11399..11884
                     /locus_tag="PK105_00735"
     CDS             11399..11884
                     /locus_tag="PK105_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOU60257.1"
                     /translation="MTIDTVKLDFPTNCNVIIGQSHFIKTVEDIAEIMMNSVPGVKFG
                     LAFCEASGPCLVRTEGTDPYLVEVAQKMAMSVRAGHSFYLVIGPGAYPLNVLDRIKTN
                     PETCRIFCATANPVEVVRALTDQGAAILGVVDGFSPKGVEGAEDKIARKAMLREFGYK
                     F"
     gene            complement(11985..12953)
                     /locus_tag="PK105_00740"
     CDS             complement(11985..12953)
                     /locus_tag="PK105_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60258.1"
                     /translation="MNPRHPMRHIPGIPDIIEPEFKKTKGFEGSETTEGGSRQTALTG
                     RDEGKSLRPPPIDYSSKEMPKTIMEANDLSIGWRQGSKLNILAQDIKFKAKQGYLIAL
                     VGPNGAGKSTLLKTIAGLQPPCGGSLLLMGKDISRISIEERARLLACVFPERIETGFL
                     TVWELVAFGRYPYTNARHRLTEEDRKQVTEALSMVGMQSFANRTVSSLSDGERQKVQI
                     ARAVAQSTALLIFDEPTAFLDAPSRIEVFKLAERLVRQAGKTLLLCTHEVDLALKTAD
                     ELWVIDRAHRFSAGAPFVIAHSGAIGKAFDLPSVAFDVFSGTFRAR"
     gene            complement(12950..14089)
                     /locus_tag="PK105_00745"
     CDS             complement(12950..14089)
                     /locus_tag="PK105_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU60259.1"
                     /translation="MSTYKDYNNRINKEMTDNQPNSARQGLLVACLAGSVLLLALLDI
                     ALGSVRIPLLEVLKSLSSKASRPEWTTIVLVFRMPKMLTAAMAGAGLSVAGLILQSIF
                     RNPLAAPDSLGIGAGASIGVAVLMFAGGSLGALGASAQGIGTQGSALLGNLPPLGYTL
                     LIIASSTGSAAVLIIILLISRRFEQVVTVLIMGLLVGYLTSSLVSLLVYFGSPQKVQL
                     YLSWTYGSFGGVRTNELPYLAIAICLGLIITLREIKALNAFLVSERFASTIGIRVRTS
                     RTKLLVAASILAGSITAFCGPIAFLGIAAPHAARLLSRKADHRILLPIAALTGACFAL
                     LADIISSLPTYGSLLPINPILALIGSPIIISMFFRRNKSSMEVSQ"
     gene            complement(14107..15276)
                     /locus_tag="PK105_00750"
     CDS             complement(14107..15276)
                     /locus_tag="PK105_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU60260.1"
                     /translation="MRRIAGIFLSLFLIFFPFFDLQQTRSQHLSAQPTSTQQTTYQKA
                     SILHASFSIEQKNGYKLLKAYNLWPGASSTKTYVLYPRGSKAPRDVAADLAIAVPVKR
                     VVLYSTTYIPAIEAIGELDAVVGVDNADYVYSPALRAQIATGKTIETSKNWMPDIERL
                     IMLKPDIIFNYGIGNEWDTFPKMQEAGLPIALIGDWNEQDPIARAQWTVFIAAFFDKE
                     SIAQERMEEVIKAYESLKTLASGASKRPKVLVNGPFQGIWSVSGGQSYMARLIKDAGG
                     DYLWADNKGTGALNLSVEAVFERALKADIWLNPVSGAKRLSDVRVMDPRFSALPVLQK
                     GQVWNNDLRCSPGGGNDYFESAVTNPHLVLSDLIAIFQPELLPNHKFNYYKRLTE"
     regulatory      complement(15302..15714)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(15801..17045)
                     /gene="proB"
                     /locus_tag="PK105_00755"
     CDS             complement(15801..17045)
                     /gene="proB"
                     /locus_tag="PK105_00755"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOU60261.1"
                     /translation="MQIHETMDEASARAALARANRIVVKIGTATISRPESSPARLTRL
                     YRSDGKAFLADLPDGAVAKDSGYPSKGIIDTAYIYNVAEQFAELVQAGRQLILVTSGA
                     IGMGARELGLSRRVTEVRMRQACAAIGQPILMEEYRRAFGVFGLVAAQLLVTRDEWDD
                     RTSYLNLRKTVETLLEARAIPVFNENDSVSTAEIGNAFGDNDRLSAYVASKIDAELLI
                     ILSDVDSLYNSDPRENPHAKPIPYVRELSEEHLSFAGGRGSEFSTGGMKTKLAAVAIA
                     RDAGCRVVIAHGRESNVIKRVACGEPLGTLFDATHALKNKIRWLKNSQPRGNLTIDQG
                     ALAAIRERNSLLPAGIVAVEGDFGRDSVVLVNEIVKLISNFSSAELRAVMHKRSEEID
                     VILGDNVPHVVARPEDMAFLDE"
     gene            complement(17060..18370)
                     /locus_tag="PK105_00760"
     CDS             complement(17060..18370)
                     /locus_tag="PK105_00760"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOU60262.1"
                     /translation="MQQRLERASLAASRMLSLETEVKNSALACIAQGLRENRDRIFYA
                     NQLDLTATRQNGLAIPLLRRLEFDKKKLDDVLLGIRDILALPDPTGRVLEARLLDDGL
                     ILKRVSCPIGLIAMIFESRPDALVQMASLAVKSGNAIVLKGGSEANESNKVLATIIAR
                     AGEEKGLPSDWLTLLETREEVGELLAFDKYVDLVIPRGSKEFVSKIKAESRIPVLGHS
                     DGICHVYVHEDADPVMAIKIVLDSKIQYPAACNAAEVLLVHAAFAHRSLMALLQALSK
                     AGVMLDVCEKAAQILDKDPGQTRNLHYSVKTDKDWAVEYLDMRMAVKIVYSLDEAIAH
                     INHYGSSHTDAIVCASSEAANRFLKEVDSASVYHNASTRFADGYRYGLGAEVGISTSK
                     IHARGPMGLEGLCTYKWLLEGYGHIVSDYASGERHFVHKELPPN"
     gene            18509..19387
                     /locus_tag="PK105_00765"
     CDS             18509..19387
                     /locus_tag="PK105_00765"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU60263.1"
                     /translation="MAQRTIGFIGLGVMGAPMAGHIRASGERLLVYSRTKSKARLVLD
                     AGAEWRDSPKRIAQECDVIFTMVGYPKDVEEIYFGSDGLIENARSGTILVDFTTSRPD
                     LAVRIYESAKVRGLSAIDAPVSGGDIGAKNATLTIMVGGEEETFQKVKPLLEIVGKTV
                     ILQGGPGAGQHTKMANQIVVAGNLLGTVEALIYAKNAGLDPKHALLSIVNGAAQSWQL
                     SNNAPRMLERNFDPGFYSKHFLKDLRIALDSAHAMHVELPMLTLSERLFAKMVNDGMG
                     ELGTQAIYLLYERGLA"
     gene            19566..22082
                     /locus_tag="PK105_00770"
     CDS             19566..22082
                     /locus_tag="PK105_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOU60264.1"
                     /translation="MSQRESEALQALARDASFWRHIYETQQAGILIQVNGIIQDLNET
                     AVRMLGGRSASEFIGKPSIETLICPEYYDCILDRTRQLRNGRPSVPPIDIRVRQLNGA
                     ELDVLISATSWIEKNDTIIETMLIDIGPLKQRERLLEAISDLASMPLKKEFFSDVNLA
                     ISLILDSMHKLYAEPHNCGFMSFVQHENSFVEEVWGASSESPQLVSSDPIDNVTLRWI
                     IENSRIITKCESCRDRLHKMPIKEDQSWQILFMPIIPEDKLEGCAFWIFYTAIDTDKI
                     EADEERRNDFIQAALIALRTFLIQRDNNRKTSELSILNKIALEVGRKTSLQEVADTTL
                     SILEREKNWQPSVIRFKSRFGETLETISYRSSLVKNVEELQKRMHLFDGLIDRPGLGM
                     TGYVIESGKPIRCMDLPSDPHYVQTDLNIKYGIYAPISIEGKVEGAIGVESKDYAFDD
                     SDLRLLQSMSEIVGMAIRSVRLIEVLQERLHWLEILHRINEQIGIETEPYKLYGFLVD
                     SAFEATKAESVALLIYNPQTDMLEKVAARSWLDRVFEIPLKPDESISGAIFQSGKMRF
                     SPEIREDPLLISRNLSLIPPGCANIGLPVTYEERILGVFHISIKAPLSFTKELVEFAE
                     MFGSYSGIVIGRIQHVRALKEAEKEIKTAYDETLKGWARAIGYRDNETFQHTLRVTEI
                     ALAIGEALSLDEKTLENLRRGAILHDVGKIGIPDNILRKPKAFTKEEEAIMRTHTSFA
                     YELLRPIDFLKDALSVPYCHHERWDGKGYPQGLKGDNIPLLARIFSVADVYDAMTSER
                     PYRHAWTREAALEYIRSQSGKHFDPRIVDVFLGIVDKIKD"
     gene            complement(22170..23060)
                     /gene="thyX"
                     /locus_tag="PK105_00775"
     CDS             complement(22170..23060)
                     /gene="thyX"
                     /locus_tag="PK105_00775"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOU60265.1"
                     /translation="MAHCIVPEAEAILDKEFPVLDKGFVRLVDYLGGDQRIAQAARVS
                     YGEGTKSYREDAALIDYLWRHEHTSPFEQVVLTFHVKLPIFVARQWIRHRTARVNEIS
                     GRYSIMRDEFFLPYEDALAPQSEDNKQGRSDMPFDKQTASRIQDILKEGSLFSYERYR
                     ELIDMGLAREVARIALPLSLYTELYWQMDLHNLFRFLMLRLDSHAQREIREYAKVILE
                     ITRKVAPAATASFERYTLEAVRFSGPEMRELRQRLMKAESLEDVATKCVESSKEDQTS
                     ELSRKELERFEEKLKSGRQL"
     gene            23185..25275
                     /locus_tag="PK105_00780"
     CDS             23185..25275
                     /locus_tag="PK105_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOU60266.1"
                     /translation="MNSKVELILDRTSGHILKIFFKAKESIECDEPLLELGEIEIKSL
                     CDALGNECQKAEEAAGFSLGSATEKLSSARKRFVLVNGWQSWSFAGELKGRERPRRAL
                     LRRDLNLFVDHPAERALRLAALRRPAQRPHTLCQRARRLFHRRDIISHFLLGLRADQL
                     RLVLVSDNVERWNSPRSTDADRAYLRDLSSCSRNESPCPDYLPPVSFILRGDKISVFV
                     YVAGGSFAAGETIARLCVFIAPDYFSLKDKLGSYWGSDRRFDDSSWLYEPKSVSHGMI
                     GGYASWYNHYTAIDEQIIGNDLDAIKANDNFVNSYFLRRGLPAVFQIDDGWEIQVGDW
                     QADPRKFPNGMEKMARRIAEKGLVPGIWLAPFLLMPNSATAKAHPEWLLRDARDKPVK
                     AGWNPNWGGDVWALDISQCDVEDYLLSLIDTIVNVWGYRYLKLDFLYAGLMQGVFSGR
                     KGGAWQHYARIMAHILEFSRASNGEPVAFLSCGAPIESTAPFMPLMRSGADTREHWEW
                     PQLRLIGHQGRPAAKVNLEDSIGRAILDKSLLLCDPDVIFCRTDLTSLKDTEKFLIAM
                     VAAMFGSQLMTSDDPALFTNHAKIDNNPDKLSEAAFTRELIEWYKHIEGKEFGIERNP
                     GRTRGLYDFFSRDGSIFGSINLSDREQLIFAELSEPSELTRLSESSESFESVAPDLSS
                     KFPMPRHSMIIFGL"
     gene            complement(25349..26527)
                     /locus_tag="PK105_00785"
     CDS             complement(25349..26527)
                     /locus_tag="PK105_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOU60267.1"
                     /translation="MSQTVRTIQLGERTIILVGTAHISKESIEEAKATIIAEQPQRVC
                     VEIDMGRYQSISQESQWEKLDIIKVLKQGNGFLLLANLALAGFQKRLGLDLGTKPGEE
                     MLAAIQTAEEMGIPWSAIDREIQITLKRAWGKSNLWNKSKLLASLIESAFSREKLSEE
                     DLEKLKESSELEQMMNELASYMPSVKEVLIDERDRYLATKIFLTTEQKVVAVVGAGHM
                     NGIEAWLEKLSRGEIEADVSDIETLPKKGWFAKSAGWLIPILIIALITIGFFRSGGQA
                     SLAMIERWVLLNGSLAALGSLLCMAHPVTILSSFLLAPLATMNPLLAIGLFAAIIEAF
                     FRKPTVHDAENLADDVTSLKGFYRNRITHILLVFFFSSLGGMIGNFIALPILASRAIG
                     "
     gene            complement(26536..27576)
                     /gene="holA"
                     /locus_tag="PK105_00790"
     CDS             complement(26536..27576)
                     /gene="holA"
                     /locus_tag="PK105_00790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOU60268.1"
                     /translation="MAKYPIPPVWILAGPEVGLKGAFIADLIDKARRLGEGEPEVHRM
                     YAGDSSPSEAIELLQNSSLFSPWVIIEFRNADQISLKPEIDTMVKYCGSPAEQALLLL
                     ETENYSVSKAIEKVVPPSCRKTFFELFENELTGWIRRELGKSQLSIDEEALESFLELV
                     PHDTNSLAAACLVLSASFPPGSMLGTSDVEAAILRSKPEDAFTLFDKIGQGDLSLSLE
                     VLNSVLDSRRGDANQIIAALVWSFRRLARIGEAVADGERFEDVCIREQARSKTVQKKL
                     RNALGNYEPEDCRRIISALSETEAAIRGLLGSAFDRQLLQLLIISIITQKGKGLPLAG
                     WSERGLYPQFIN"
     gene            complement(27581..28183)
                     /gene="lexA"
                     /locus_tag="PK105_00795"
     CDS             complement(27581..28183)
                     /gene="lexA"
                     /locus_tag="PK105_00795"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOU60269.1"
                     /translation="MKDLTDRQKLILSFIREYIQKNNYPPTVRETARAFSMSVKGAYD
                     HIKALEKKGYIKTTEKRSRAIELVSPPEDNTAVVKIPLLGEIAAGKPIFADENFEKTI
                     SIPAEFVGNHIPHFALRVNGDSMVGAGILSGDIAIIEQSETARNGDIIVALLDENVTL
                     KRFYIERNRYKLQAENPGYAPIYTQDLKILGRLRGIYRSY"
     gene            complement(28274..28546)
                     /locus_tag="PK105_00800"
     CDS             complement(28274..28546)
                     /locus_tag="PK105_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOU60270.1"
                     /translation="MVELETTIKNRAGIHARPSALIAQTAIKFASRIYLEKEGNQINA
                     KSIMGIITLAASFGAKIKIIADGADEQEAVKAIAELFESGFNEELG"
     gene            complement(28644..29642)
                     /gene="hprK"
                     /locus_tag="PK105_00805"
     CDS             complement(28644..29642)
                     /gene="hprK"
                     /locus_tag="PK105_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOU60271.1"
                     /translation="MKEKEPVNFTVLDLLSLELKEHNDLKLQCTAGRAGLSREISVPD
                     LNRPGLALAGFYDSFAWERLQIFGRGESAYLHKIEEDGVAAQIYEKLFSYQIPCCIFT
                     NGIRPTDLFVSNAEAVGCPVLITELNSMELSIRIIRILHDIFAPKMIMHGVLVEVFGI
                     GILLTGESGVGKSETALSLIERGHRLVADDVVEIHCLNGSLLMGQGTNKVIGHHMEIR
                     GLGIINVTHLFGVGAIRDKKQIQLVIQIEDWDPKKVYDRIGTDELTMEILGVKIPKLE
                     IPVKPGRNLAIIIETAAMNERLKKMGYHSAREFNQNILRWLESESARTMFFSRDDY"
     gene            complement(29793..30089)
                     /gene="raiA"
                     /locus_tag="PK105_00810"
     CDS             complement(29793..30089)
                     /gene="raiA"
                     /locus_tag="PK105_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOU60272.1"
                     /translation="MNIDVRSVHFDLSEQSREYLNTKISRIDYAKDLIVDLLFVFTKD
                     AGRFRLEVTANFRWGKQAHLVVTSPELNAGIDVLIDKLDHKIVKEKEKIQKKNK"
     gene            complement(30155..31504)
                     /gene="rpoN"
                     /locus_tag="PK105_00815"
     CDS             complement(30155..31504)
                     /gene="rpoN"
                     /locus_tag="PK105_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOU60273.1"
                     /translation="MQSQRPVLVQQQRLRLNPRMVQSIHLLTLPLQELTEQINQELQE
                     NPALELLTGSSEISLDFMESHIDNMAEKENSLNEGEYSDDIFGFNTDDIDHNAIIEQT
                     IATEESLQEHLLSQLGLLPLNEQERKIAERIIQNIDENGFHILPPSELCNDCDPETLN
                     RLLYLLQRLDPPGTCTSDFRESLIVQTEISPSAPPKTIDVIKDHFEDLRLRDRSSIKK
                     ALGLDDRQIDRIVEFIRTLEPFPGRSFSKARPRYIVPDIAMWLEDGEIKVRLNDDFVP
                     KLGINRLYSELASQPAKGEAEKFAREKVENARFFINSIKKRNETLLKTAYAIAETQRD
                     FFVAGPKALKPLTLKEIAEKIGVHEATVSRVANGKYVQTDWGTFEMRRFFTNAINQEK
                     GKNISKEAVKAEISQIIQELEAKGEKISDRIISEILARRGMKIARRTVAKYRGELGK"
     gene            31705..32436
                     /locus_tag="PK105_00820"
     CDS             31705..32436
                     /locus_tag="PK105_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOU60274.1"
                     /translation="MSKHKHEQEQQSEIRKQENLQDQNARNAPSAQESTMEHGADKQQ
                     LNQSTSDLESQLAAANEEIARLKAQLDELNDKYLRILAEQVNFRKRVVKEKEEFQQYA
                     VSMLLNDLIPVLDDFDRSLDAVAQNHNDVEKVIEGIRLIQKRLYDTLSKKYGLKQYKA
                     EGTVFDPHLHEAMFSEKGDVHEPRVTQEFMPGYKLHDRVIRTAKVKVMMPANPAEAPS
                     RATEVSGETQDSSFFESTEENSALG"
     gene            32506..34440
                     /gene="dnaK"
                     /locus_tag="PK105_00825"
     CDS             32506..34440
                     /gene="dnaK"
                     /locus_tag="PK105_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOU60275.1"
                     /translation="MGRIIGIDLGTTNSCVAVMEGGEPIVIANSEGQRTTPSVVAFTA
                     KGERLVGQPAKNQMITNAENTVFSIKRFMGRRYSEVLNEIKLVPFKVIDNGNDVRIEA
                     GGKLYSPQEISAFILQKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIAGL
                     EVKRIINEPTAASLAYGFNKDQKKEKTIAVYDLGGGTFDISILELGEGVFEVKSTNGD
                     THLGGDDFDRRIVNWLVEEFKNDTGIDLSHDRMAVQRLREASEKAKIELSNMQQTEIN
                     LPFITADANGPKHLQKSLTRARFEQMCMDLIERSREPCIKALADAGLSASQIDEVILV
                     GGMTRMPRVQQMVREIFGKEPSKGVNPDEAVAIGAAIQGGILGGDVKDVLLLDVTPLS
                     LGIETLGGVFTRLIPRNTTIPCRKSQIFSTAADGQTAVSIHVLQGEREMAAQNRTLGR
                     FDLVGIPPAPRGVPQIEVTFDIDANGIVHVSAKDLGTGKEQKIRIEASSGLNEAEIEK
                     MVKEAEAHAEEDRRERERIDARNEADSLIYSTEKSLKEVGDKISASEKSAVENAVKEL
                     KDVMEGKDTELIKQKTEALKQASYKLSEELYRSASAQGAASEASGSATGESASDGSAG
                     SSDNAQNADDVDYKVVDDDK"
     gene            34506..35630
                     /gene="dnaJ"
                     /locus_tag="PK105_00830"
     CDS             34506..35630
                     /gene="dnaJ"
                     /locus_tag="PK105_00830"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOU60276.1"
                     /translation="MAKRDYYEVLGVPKTATKDEIKKAYRKLAIQYHPDKNPGNKTAE
                     EKFKEATEAYEVLGDDQKRQAYDQFGFAGVEGMAGAQQDYSSVFRDFEDIFSGFGDFS
                     SFFGSIFGEGSQRRGETRTNHGANLRYDIELPLEKAVFGTTVEISYNRDDVCKTCGGS
                     GSRDNLGRKVCSTCKGTGQVRRSSGFFAISQPCPACHGEGTVIENPCQDCAGSGVVKK
                     KQKIRVTIPPGVEDGKRVTVPGQGNAGPNGGPPGDLHVFIHVRPHETFERQGNDLYCA
                     VSIDFVTAALGGEVVIETLEGRKISVQIPAGTQNAKLLRIKGEGVPVSGGKRGDLYIK
                     VFVKVPSRLTKRGRELLEELKKIEGETLQPELVKLKDIPR"
     gene            complement(35676..35885)
                     /locus_tag="PK105_00835"
     CDS             complement(35676..35885)
                     /locus_tag="PK105_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOU60277.1"
                     /translation="MKKLLKIEGMTCGHCVMHVQSALEAVPGVRSAKVDLLQRQAIVE
                     GEQLDDQALRAAVTEAGYRIVEIIP"
     gene            complement(35981..36166)
                     /locus_tag="PK105_00840"
     CDS             complement(35981..36166)
                     /locus_tag="PK105_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60278.1"
                     /translation="MADRKTFAIKGMTCAACVAANDLAVNKLNAVTNISVDSVQACYM
                     LLAGFCFRQFLRQPPCP"
     gene            complement(36210..38315)
                     /locus_tag="PK105_00845"
     CDS             complement(36210..38315)
                     /locus_tag="PK105_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU60279.1"
                     /translation="MAESNAKKHNILSHLRQLRPIEHFWLVVFFIILLGTLMIGGYCQ
                     WQNILHDKKLYIELIGKSKISQIEIWYTAHITEAKEMPHSLPFLNLVSRAIAEPSTDN
                     LELLDESLRPSLLSYNYADTAVLTSSFDVLYTLTNLSTSNSSEVVREISERKPKTPFF
                     TRMYLTSPHAKAGFHLVIPLIWEGEEEPFAYVVHTFLAEDYFFPLLATWPGTEETGET
                     MLLESSNNMVQVINPLKLVPISAFSLQISIAAENSVEAQAGLGKTGVMMGKDYRGKQV
                     LAYAEKIPNLDWIILSKLDYAEAFASLIPTIVVFIIFIFVAIIALLAGSYVIFSTRAL
                     ATFQSKFELLKRTERNEALLSTILERIDFPVVAIDENLEIRLANHSFRDHFGDTLPKD
                     LRLPHMDTKTAQTEVQRIEIAGPQGQTLRFDSTEIQIALPDQPMLFVYVMRDVTNLET
                     MLEQVNNLNRELAQKVEEQTKCLSDTNDELRAIASAISYNLIAPLYNIESLSKSLEAL
                     AKNKSDSEIMSYITNIRKTTESMTRLTEALSIFLSIDTIQLADEEFDFSIAAQEITSE
                     IIKRNPKRRYQITIMPDLKVHGDGNLLKIALRNVLENAIQNCPETLTAMIEIGKCGES
                     CIFVQDNGIGISPEEVYSLLKPFSNTENGQSAGQINVDFAITKKIIERHGGKLEIISE
                     LDKGTVVRFDFSSKSTTLR"
     gene            complement(38582..40585)
                     /gene="tkt"
                     /locus_tag="PK105_00850"
     CDS             complement(38582..40585)
                     /gene="tkt"
                     /locus_tag="PK105_00850"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU60280.1"
                     /translation="MNIAALQAIALSVRSLTIDAIQKANSGHPGMPMGAAELGAYLYG
                     VELKYNPKDPKWLNRDRFVLSAGHGSMFLYSLIHMAGFDLSLDDIKAFRQVGSKCPGH
                     PEYGRTPGVETTTGPLGQGIATAVGMAIAERKMMAHFNTPGHQILDHFVFALAGDGCM
                     MEGVASEACSLAGHLGLGNLIVYYDSNHISIDGPTDITFSEDVGRRFEAYGWQVLRGS
                     MYNFEDIASLTAEAKAEKDRPSLIILDSIIGKGAPTKQGTSGVHGSPLGDEEARKTKE
                     GLGIPLDKPFWVAPEAYSYFKEHQGELEKEYDAWQLRFEAWQKANPSLAQELTIWLSG
                     KEMHKLDMPSFAVGDKVATRNASGKCLAAISTAWPNFIGGSADLTNPNVSQLPQNTDG
                     TPLVFSKTNPMGRYIYFGVREHAMAAIANGIALYGGLRPFVATFLVFSDYLRPALRLS
                     ALMRLPVIFVLTHDSIYVGEDGPTHQPIEALAALRAIPNLKVLRPADADETSASWKIA
                     MERLDGPTVLVLSRQNLPVLEKHESDWSSKIRLGAYVVKDSDRAPEVTLVGSGSEVSL
                     AISAAEIVKSKRHETAIRVVSVPDRGAFYESCPMVREIILSPGSKVFVAEAGVAQGWE
                     RIAPSDQILSIERFGESGPGDKVAEYLGFTAQTLARMILAKLD"
     gene            complement(40766..42673)
                     /gene="uvrC"
                     /locus_tag="PK105_00855"
     CDS             complement(40766..42673)
                     /gene="uvrC"
                     /locus_tag="PK105_00855"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOU60281.1"
                     /translation="MNARYAKKCDDKKSPQARDRDAIREAIQPLKGPIVETDSERRAR
                     LKALVKQAPECPGVYLMRDENHEVIYVGKAKVLKNRLSSYFAGKKDAKTRHLVSHIHE
                     IEWVLAAHEYEALLLENNFIKEYSPRYNVSLKDGKTYPSIRITNEEFPRVFRTRRIIN
                     DGSEYFGPYPSAETIDTYLDLIKRMFPLRRCANIKKHTSPCMYYHIGRCLAPCADKIS
                     LEEYTKQVDKVRKLLLGETESLVRDLEHDMNRAAERLRFEEAARLREAIKALRSFAGR
                     SNVVDFNSPGARDYIAWEADGDMASYVIFQMRDGKLKNRDSFMEHCVDELDALDSFLA
                     RYYDIGRQPPPQVFVRRADNTATIARYLSDRAGTPVEVKIPDSERHWAVMNMAVQNAK
                     EELAKRRRAHGDIVALVELKERLGLGSVPMRIEGFDIAQLGGKHTVASLVSFIGGVAE
                     KKNYRYFRIRSVEGKVDDFAAMREAVARRYTRLINEEAELPDLILVDGGLGQVSAAKE
                     ILDDLGLDIDIIGLAKRNEEIWLPDKNDPIVLPKDSPSLRILVAVRDETHRFATGLSR
                     KLRSSDATLHMLQEVPGIGDRRAKKIFNALGSLEAVAEAEPGYLSSIARVNEDTARKI
                     IELARRNVGIE"
     gene            42701..43444
                     /locus_tag="PK105_00860"
     CDS             42701..43444
                     /locus_tag="PK105_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU60282.1"
                     /translation="MEKTKQQSEASQTPREPFAIMASSELPIIDIDNNGCSDEYIRSL
                     EEKTAHALERLGGIGAAASAFHFCPSCGSPKLYSVRSRMWKCPTCGFEYFHNVATAAG
                     VIIEAKNKIIMIRRHKDPMRGRFALPGGFTEPGERAEDAALRECYEEIGWSPTHINFL
                     ATFPNVYQYQGIPYATCDVYFYSRGNIVEIDDFHLDPEESDSVQFVAANAIPWANIAF
                     ESTIRALRYFILHESIGTPPRLPEFGAIE"
     gene            complement(43523..44509)
                     /locus_tag="PK105_00865"
     CDS             complement(43523..44509)
                     /locus_tag="PK105_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU60283.1"
                     /translation="MDFVRWGILSVSNHYRLRVHHQLSGSKLTKMFGIASRDLEKAGQ
                     AASELGIARSYGSYEALLEDPEIDAVYIPLPNHLHAEWIKKAAEAGKHVLCEKPFAMN
                     AAQAQDALLYAESKGIKVMEAFMYRFHPQWVHAEEVVRAGEIGKLQYVHVQFTYNNRD
                     PSNIRNILSAGGGAIYDIGCYACSSSRFIIGKEPRRAISLVRRDVEFGTDVLSSAILD
                     FGDAEALFTVATQSFPVQRVDIVGSAGAIAIFIPFNMYDDIPAEMSISTSIGSRIVRL
                     GPAGQYRLMFDAFSKSIIEGRAVPTPPEDAIANMKVIDALFKSERSGFWEDV"
     gene            complement(44534..45718)
                     /locus_tag="PK105_00870"
     CDS             complement(44534..45718)
                     /locus_tag="PK105_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU60284.1"
                     /translation="MNELEKQQNISRRFGLGYFMLFAMYGISSPYLQLMVRRLGYSPA
                     TVGFFLGFFELVGITGPIFIGQIADRFGKLKPFLYASAIMILVGLALLAPFRLPLVTL
                     LSLALLSIGIKTPIPVLDTSLLRAIEYSEEKGEKAPNYGIIRAIGSAGFVITALIAQS
                     IPGFDSSSTGTMAIPLGILALLFLLSIPSLPEIGAGQARKEKIKFSFKWLDATYLAGL
                     GVIALGRLAMAPISSFFSMYLMEELNWHAVGSMWALSAACEIPFIVLSWKLIQGRSPM
                     MAIAISSVAIVVRLLIYALFPTPIGVILGQLLHSICYGLFQPAAILFINLKTPPSDRT
                     TGMALYMGVGMGLPAFLGSALGGTVVEIFGYRWLFASYTLFAVASILLFWRFRKELKA
                     VR"
     gene            45802..>47080
                     /locus_tag="PK105_00875"
     CDS             45802..>47080
                     /locus_tag="PK105_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU60285.1"
                     /translation="MEMPIISLLHAHCPSNQKTKKFLMIAGLLLGDLFAVMASFCSGF
                     FLENLIDIEGINFKSFVTYWPYLPAFIVMLSLLNLYPGITLSTPEKLRRFTVASFLAH
                     AGIILSLFIGAKRFDQYQTAFLFSWVFSIPLFFYCRTVSESLLVRYRLWGIPAVIFGA
                     GKTGKLVVDRLLDNLKIGYIPVLLLDDNPLLAGEYRGVPIVTGTNYGSFIASSGEVQT
                     AIIAMPGVEHKRLTEIIVRQAMGFRRYLIIPDVIGISSLWMAVQDLNGILGLFTDQEL
                     LEPRNIAIKRLLDIVLSVIGMILLSPFFIVIALLIKLDSPGPIFYSHHRLGKNGKPIE
                     ILKFRSMYTNSAQMLETLLRDNPELQTEWQANFKLKNDPRITRVGAFLRRTSLDELPQ
                     LWNILKGDLSLVGPRPIIDNEVEKYGDNYWLLSK"
BASE COUNT        11993 a        11273 c        11625 g        12089 t
ORIGIN      
        1 gtcataatgc ctccaggctc gaatcctcat tcttttgaac cctcgcctcg ccagctcgca
       61 gaattgggaa acgcgaagct atggttttcc atcaatgtag actttgagta ttcgcttaaa
      121 cccaaattgc gcgccatgtt tccaaagctt cagatcatag atgtgacaaa agatgtccaa
      181 ttcagaaagc ttcggccagg cgagaaggaa catgaccaag atgcagttga caaatatgca
      241 gaaaattccg atcccgccct tgtaaatctc gatcagcata cctggcttgg tattgagcaa
      301 gccaagacag agatcatggt aattcaggat accttgatag ccatagatcc tgaaaataga
      361 gagctataca agaccaattg tcaagactat ctgaaagata tcgatgccgt atactcagca
      421 ctgaagacaa agcttgcgcc gctttccggt gctcgagtat ttgtatatca tccagcattt
      481 ggctattttc ttgatatgtt cgatattgaa caggttgcgg tcgagctagg tggcaaggaa
      541 ccgacgcaga gagagcttta cgctctcata gagcttgcaa agaaagagaa agcctgcgca
      601 attttcactc aggtgcaatt ttcagaaagc gctgcaagag ccattgcaag cgcaatagga
      661 gccgtggttg tgccgatcga ccctctagct tccgactggc tagaaaattt gtccagaata
      721 ggccaggcct tgtctatgtc aataggcaaa agagaataga atgaaccagg agattaaata
      781 tcggatagcc tcgctaaggc aaaggacggg tagcctgaat tccatagaag gcaaggaaca
      841 tggtgcttct atcgaaccta cactgcctca tcctccggat aatgttgcca tctggtgcca
      901 tgatctgggc ttttgttatc gcgaagcaga agtgttcaga aatgtcagct tccatgtaca
      961 tagcggcgaa ttcgtagtgc ttaccggcaa gaatggagcg ggtaaaagta ccttgcttag
     1021 actcattgta ggtcttctgg aaccacagca gggacaagtc cttgtgtttg gcgtctctcc
     1081 tcgcagacga attgaatcta taggttatgt gcctcagcat acaaactttg accaagcctt
     1141 tcccattcga gtcgaagatg tcgtaggtat gggaaggctc tccgggcttt catacatttc
     1201 gaaagaccaa aaaaaagtgg atatcgagtg ggctatggag caagcagaag ttacggatct
     1261 gcgagcacgt ccattctctg ctctttctgg gggacagagg cgaagagtcc tggtagctcg
     1321 agcgctagta ggacatccaa ggctccttgt catggatgaa ccaacggcaa gtatggatat
     1381 tgctagcgag aataaattct tcgaagtgct ggctcgcata aagagaagca caacggtttt
     1441 gatcgccact catgattcga acttcgtgtc aggacttgcg gataccgttc tctgtgtagg
     1501 tcaggattcc gagcatcctc actcggtaca ccgacatgcc atggaaaagg cggaagatct
     1561 gccaaaggac atatacggaa gcaacatgct tcgtgttcgg catgatatcg agctgccgga
     1621 caatgcttgt tgcgaaggaa aagaccaatg agcagcttct tccaggcagt aataaatcct
     1681 tctattccct ttgtcagaaa tgctctcata ggcagcgtgc tggcagcttt tttatttggc
     1741 atacttggtt ctctggtcac cgtgcgtagg attgcagggc ttgctggcgc gatttcacat
     1801 acggtattgg gtggcatagg gcttgctctc tttcttgttt cgagaggtgc tcctgagtgg
     1861 ataagtccca tgcttggtgc gcttgtattc gccatccttg ctgctattac gatcagtatc
     1921 gtgtcgataa aggcaaaaca acgcgaggac actgtcatca atgcgctttg ggccatcggt
     1981 atgagccttg gtgtgatctt tatggccaag actccgggct atgccgatcc catgagctat
     2041 ctcttcggta atatactcct tgtgactcag aagaatcttt atatgctggg agcgctgaac
     2101 atattagtat tgctgcttgt atggcgatat tacagacaaa tcgaagcagc ttcttttgat
     2161 gaggaatttg ccgctactaa aggtctgccg gttatggtca tatacctcgt ccttcttctc
     2221 gtgatcgcgg tcgcggtagt catgcttcaa acctttgtgg gcatagtcat ggttattgct
     2281 atgctgactt tgccggcggg aacggctggc tttttttctc gcaatctagc taagatgatg
     2341 ataaattcga gtattctagc ccttgtcttt tctgtgcttg ggctgattct gtcatgggtt
     2401 ttcgatttgc cggcaggagc cttgatagtt ctaattgcgg gtatggtcta tatcgggatt
     2461 tcggttgtca gaatgctaca aggtcgtaga tcgtcgacat catgatcctc atagtcgagg
     2521 tgggcaataa gctatcgcga gctcaggcaa ggaaatcggg cagaaatttc aagtcgggag
     2581 ctcgagccag gagaattcaa tgacaaaagc gcgtaatcat gttttggaga gcatcattaa
     2641 agccgcagag ccactttctg cacgtcaact ctgcgataaa tacaaaagcg agcatgatcc
     2701 tgcaacaatc tacaggtcgc ttcatttcct cgaagaaaaa ggccgcatcg attcctttat
     2761 attatattgc aatgcgcatg gaacagagcg ttattatgtg cttcatcgcg gcgaacaccg
     2821 gcactggttt cattgtgagc gctgtcatcg ctttaccgac ctcggagagt gtcgatttgc
     2881 tgagattgtc tcgcagatta gcgctgaaaa aggacttttg ataacttcgc actcatttta
     2941 tgctattggt atatgcagcg agtgccgcca acagggttca tagtattttt gctccacaaa
     3001 gaagctgcca ctatgaatga agaaaaagaa ctcagcactt acctcgagct cctcgcccac
     3061 ctcaaagaaa aggacctggc caagcaggga ctaatggccc ttgaaggccg aattgtcatc
     3121 gaaaaggcgc tggaagctgg gattgtaccg cgtatccttg tgtgcaccga ggcagaaaaa
     3181 gactattggc atgaaagaac taaagaagag aattcgatgg ttggtgccca cacccccaga
     3241 agtagagagc tagactttcc aatatatacc acaagccatg aagcactttg cagcttggtc
     3301 gatttcaaat tccatcgggg agcaatcgcg atggcggatg tgccagagtt agagctgttc
     3361 gacatggaac ataatagcca actccaccag tttcaccgag ctcattgcac ccatgcggtt
     3421 tggtctccca ccattgcagg agttcgtgcg cctcttggga ggcggcgacc tttttacctc
     3481 tgtctttgga atgttactga tccatctaat ctcggcgctc ttattcggac cgcggcaggg
     3541 ttcggtgtcg atggggtgct cataggacca ggctgcgcca accccttcta tcgaaaagct
     3601 attcgggcat cgatgggaaa tgtctttgta ttaccccttt ggagcgtcga tgtgcacggc
     3661 cttgaaaagc tcaagcaaaa tggcgcagta ctcgttgcgg cagccctcga tgagaactcg
     3721 atggcactgg aagagctcgt aaaggaactc gaaaaacttg aaagtagctt tggtgcgtct
     3781 gaggacgagt atataaagga tgagtcttcg gtagtaggat gcgctcaaaa ctctccaatt
     3841 attctccttc ttggcaatga aggctatggc ctacctgcag aggtgctcgg gctgagctct
     3901 atccatgtcc gcattccaat ggaacgcggc gtcgattccc tcaacgtggc agttgcaggc
     3961 gggattttga tgtatgaaat tattcgcgcg cctcaatcgt tatgctgata ttataagcta
     4021 ggcccttttt tcaagaccgc tctacgaaac ttttgaagtg gatactcccc tgcatgcgac
     4081 tttcatcgct tgacgagatc cgcaagcgcc ttgttatcga gttcggtgat ccaagtttcg
     4141 cccgcggaaa tggccatctc ggccaaatcg cgcttggtgg tcaggatagc atcgattttt
     4201 tcctcgaagc tatctttggt gataaggcga tgcacgaaca cgttcttgcg ctgaccgatg
     4261 cggaaggcgc ggtctgtggc ttggttttcc accgcggggt tgaaccagag atcgaaatgt
     4321 atcacgcgag tagcctcggt aaggttgagc ccaaggcccc ccgccttgag gcttatgagg
     4381 agaatcgctg gccccgaggc attgcggaaa ccctcaatcg cagcatcgcg cttcgtcctc
     4441 gtcattttcc catgcagcat gaaaggttcg acgcccctat cctgggcgat gatctgcctc
     4501 aaaatatcca gcatttccac atactgactg aaaacgagca ccttttcgcc cgcttcggcg
     4561 atgctttcga gcagagagag cagtaggcgc gtcttgcccg accgctcggc cgagggggcg
     4621 ctgtccttat cgtagttcct cgggtgattg catatctgct tcaatcctgt gataagcgcg
     4681 agaatgaggc cccgccgagc aattccctcg ctatcggcga tcttcttcat acaatcgcgg
     4741 acggtactct cgtacaaggc agcttgttcg gccgtaagcg tcgcgtattc gttcactacg
     4801 attttatcgg gaagatcggc gatgatggaa cgatcggttt tcaaacggcg aagcaggaaa
     4861 ggcgcggtga cgcggcgcag tcggtcagcg acctctttcg atttgtcgac ttcgattggc
     4921 ttgcggtagt tcgaccggaa atgctcgagc gtatgcaagt agccgggtat gacgaaatcg
     4981 aaaatgctcc agagttcgcc caggtgattt tccacgggag tgcccgagag ggcgagcctg
     5041 tcggatgcca cgatttcctt caccgcctta ctgcgtttgg cttcgggatt ttttatcaga
     5101 tgcgcttcgt ccaagatggc gatatcccat ttcttgtcct taaatttttc cttgtcgcgg
     5161 agaaaggttt cgtaggaggt gaggaggata tcggccgatt tgtggtgtcg tccagctccg
     5221 taataggtgg aaacgctgag agatggcgcg aacttttcga gttcgcgctc ccagttggtg
     5281 atgagagtgg caggagcgca tatcaaggcg ccatttgtga gcgatttcgt ttccttgagg
     5341 tgaaggatgc aggcaatcgc ctgcacggtt tttcccagtc ccatgtcgtc cgcgaggata
     5401 cagccgaagc cacgttccat cgtcgtggcg atccatcgga agcctctcgc ctgataggga
     5461 cgcagcgatg cgttgaggct cgctggaagg ggaatgtcgc tgtcgggcat agcgccgtcg
     5521 gacatgacat cgtgaggcat gacatccgag gttcccacct tggcctgtgc cgcgccatcg
     5581 atcgtccgag ggagcatcgc ggcgagcttc tcttgcagcg cttggtcgaa ttcggcggtt
     5641 ccagccaaat attcctgtag tacatcgaaa gcgtcgggct tcggtccctt cttgattcgc
     5701 tcaagcatcg aggccgcttc ggaagcctcg agacgtagga agccctgacg gaatcgcaca
     5761 agcgcagtcc ccgcctttac cattcgctca aattccgctg cgctgataag tctatcccct
     5821 atggatactt tccattcaaa ggatagcagg ctgtccaggg ttaggaagct cactaaattg
     5881 ccggactttt tgcctttttt cgcggtgagg acggctcttg gctttatcag attacagagt
     5941 tctttgggaa gggcgatggt gacgcccaga ttccctagta gcgccgcgga ttcaatgaca
     6001 aaacgcgata aagcaatttc gtccagtatg acgctcgctt tcgaacccaa ctgagccagt
     6061 tctggaacga atgtggacag gagggctgga aaggtgaggg cggatatgcc gaagcgttgt
     6121 gccgcttcat gaaggggaat acgttcttcc tgagcggttt tgactttgtt ggtcgcggta
     6181 gagctttggc gcacggttgc cgaaagctca taccgcggca tgccatccgg aatcgagtca
     6241 tcttcgtcgc cccagttaga agtatcgatc tctccgcaat ttttcacgac gagctcatac
     6301 tggaagccgg aattggcgag atcgaagacg gaaagccatg aacgcatcgc cttgggaatg
     6361 gaacgatgaa gcgggctgga gacatttgca tcctcgtttc gaaacagagt cgccgtgaca
     6421 gggtgggata tatccttgac acctgaagga agatagcgaa gttcggtaac gatatccgag
     6481 agtatgtccg aggctaacaa tagcagggtt gcgtggggag aaggaactct gcttttttcc
     6541 ctcggagcca cgctgacctt caccactttc agggttgaag cattcgatac gggttccccg
     6601 tgggcggcgc tttcgtcgat aaagaccgga actggtacga gtggttcgat taaggcgaag
     6661 agagaacgga tatcggcgct gaaaagcgcg ggtttccaga ccacacgcat aaaagtgcca
     6721 cctgcgcgct ttcccttttt tacggcatca tacatcgcga tatcgggcac gaatgccttt
     6781 gcggcaatga tcgagcgcat cgcacgcgaa aattcgcgca gaaatcggta gcctagactt
     6841 ccttcactcg tatcgatcgc gagacagagg cgcacggcct ccaaagctga taatttttcc
     6901 tttttccctg aggctcttat cgccgtgata aatggttttt tcgactgact ggatttttcg
     6961 cctctaaaaa gatgaatctc tatgcgcgat tgcgggaaag ttctggccct ttcctcgagt
     7021 tcgtcttccg gaaggatcaa ttcgagtatt cgatcccaat gctggtctat atccctataa
     7081 aacccggcga gcgcagaact gaggtcataa ggggcgaggg gcgggggcgg gggaagaagc
     7141 ccaaggattg tcttgtcata tgcccgaatt cccggaaatt ccagcatctc cattttttca
     7201 gaagtcttcc aaggtcttac caggtcgata ggcaaaggat cgggcaatag ctcgacttca
     7261 tcccttgcta tgagtttttt ctgggctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnngaa ttccgaaaaa atatcgactc cccgcagacg aaaaagcgcg gaaggatccc
     7441 ggtcgatttc ctgagccagc acataataga cagctgccat gtgcttgcag gggtcgccat
     7501 agtctgggca ggtacaggaa cgtcgcatat cgttccatcg ctcaggcagc aatcgcacat
     7561 tggctcttcg tagccgatcg atcaattcag cgggaagctc gccgatcgct attcgtccca
     7621 ctagcatggg atcatcgttg atgagtcgaa aaagtgcggt cctttccgat tgatttagag
     7681 gtttgaagac gattcttacg tcataccagg gtttatagtt gccttccacc attgccgaga
     7741 ctaccccatt ctcgatggat agtctgaaca cattcccatt ggccgcgtaa cttcgtccac
     7801 gcttaaggcg cgcctgatcg gcaaggctct ccatggctcg tataaaatat gctccccagg
     7861 gggtgagtcc gaattcatat tttcgcggca tagggcgagg gtagcaccac gcggcgaatt
     7921 cagctagcct gcggtgtcgt tttctaaact tgaaaaacgc cagctttctc ctagccttcc
     7981 tcggctagcc acgaggagtc aataagtgat aaatttatcg accgcgccat tctcggcctc
     8041 ttccctgagc gcctgagcta acgagcactg ttgtgaccca gagataagcc tcctctatgc
     8101 gttttacaga ggaagttcgg aaggaatgcc tgcgtacttt ggataatgta ggattgcgct
     8161 atgcggtgcc cttatggtgc ccttacgcca gaataccctt ggctataacc gaacgcggct
     8221 ccggtctgtc gataaatcag gattcgatgg cgaattcgag tttggtggcc gatctacctg
     8281 gtattgccat acccatgaag atacccatca gagcatcctt gtctacctcg atggagcatt
     8341 gcggcataga atcatctaca aagttcttga aacagagatt attgtcacga ttcttgcgaa
     8401 gcgtcgaaaa taggctataa tcttcgcatg gaccgcaatt caacctatat tcttgccctg
     8461 gatcagggca caacgacctc gagggctatt ttgttcaatc atgatggaat tatcatctcc
     8521 gtaaagcaga ttccattcaa acagattttt cccaagccag gatgggttga acacgaccct
     8581 gaggagattt ggcgcacgca gctaaaggcc gcgagaaacg cgattgaggc agcgcagatt
     8641 gaaccaacac agattgccgc gataggtatt accaatcagc gcgaaacgac tattgtgtgg
     8701 gagcgttcca cgggtaagcc tgtctataat gcaattgttt ggcaatgccg tcgatcgagc
     8761 tctatctgtg atgaattcaa agcgcagggt gcaagcgatt tgatcagacg aaaaacaggt
     8821 ctagtgcttg atccgtattt ctctggcaca aagctcacat ggctcttccg aaactatcct
     8881 gaaataaagg cgcgcgcaga gcgaggcgag ctgatgttcg gcactgtcga ttcctggctt
     8941 atctataatc tgacagaagt ccatgcgacc gatccttcca atgccagccg cacaatgcta
     9001 ttcgacataa acgaacgccg ttgggatgag gagcttttaa gccttcttga agtacccgct
     9061 aatattctgc ctgaggtaag gccttcgtcg gctctcttcg gtgttacgaa gaaggatata
     9121 tttggcgccg aaattccggt agctggagtt gccggcgacc agcaagcagc acttttcggg
     9181 catgcgtgct tcgagccggg agatgtgaag aacacctatg gtacgggctg cttcaccttg
     9241 atgaatacag gacctttccc cgttgaatct aagcataatc tgctcgcaac ggttgcgtgg
     9301 gatcttggca aaggttacac ctatgctcta gaaggttcgg ttttcatagc tggagcggtg
     9361 atccagtggt tgagagatca aatgggtcta ttaacggact ctgcggagag tgaacgattt
     9421 gctcgtatgg tagaagatag tcagggagtc tatattgttc cggcctttgt gggcctgggt
     9481 gcaccttact gggattcgga ggtgcgcgga actgtagtag ggcttacgcg aggaacatcg
     9541 aaggcgcatt tcattcgtgc agcgctggaa tctatcgcct accaatccat ggacctattt
     9601 gaagctatgg aaaaggattc ggggaaacgc ctttcttgca taaaggccga tggaggagct
     9661 tcccaaaatt catttctcat gcaatttcaa gccgatatca caggaaccag ggtcgttctt
     9721 ccagaagtga gcgagacaac ggcgcttgga gcggcctatc ttgccggtct cgcgataggc
     9781 tattggaatg ggctcgatga cgtgcgcaag aactggcgca tgcgcaggga attcagcccg
     9841 cgaatggcag aagaatcaag ggtcggtctt gtggtaggtt ggcgcaaggc agtagctaca
     9901 gcgaggaatt ttaagcaata agtacctttg acagtagcca tgacatatgc aatcacatat
     9961 acaatgacag agtaagtgca gattataatc ggaggatgga aatggaaagg gcggatgaag
    10021 cagtatcgat gcacgtggct ggatcggcct gttctcaagc tgtgtttacc gtatttgcgg
    10081 atgagctagg cttagagcct tccgtagctc atcgtatttc tgggggattt ggaggaggaa
    10141 tagggcgcat gggtttgacc tgcggtgctg ttacaggtgg aatcatggct ctttcgcttg
    10201 ctcttggagc aactgattcg gctgatcagg atagtaaatt agcgctttat caggtcgttg
    10261 cggaatacat ggagaggctt aaggcattca agggcagcat cgaatgcagg gtccttttgg
    10321 agggcgtaga tctatggact gaggaaggtc gggatatggt caaagccagg cagctctccg
    10381 aaatagtctg taattcgatg atccataagg ccgttgaaga agcagagaga attttgaacg
    10441 aaaaggacag atttggagtt tgatatgcaa tacgagcgtt tcgaaggaag ttggtttcta
    10501 aaatccaact ggcatttagt aaagacaggg caatcggatc agtcaaaggg agtacctgtt
    10561 cctccacaag aggaaccacc tgcgcccgaa gactgtgtga tcaacctgcc gcctcccgat
    10621 agcattctaa gggcttttga agagagccgg gaacagaagc gagctgcagc ctcattcgca
    10681 gacacgagga cctctgaaaa acctttttgg ggtgtgcctg agcgcgccgc cgtgctctac
    10741 cacctcctca ataatcgcag atcgcgccgc aaatatacaa tggagccgct cacgatcgaa
    10801 gagctctctt atctcctgtg ggcaatcgaa ggcgtaaaag agaatcgcgg caagttttct
    10861 tttcgcacca ctccctccgg cggcgcgcgc caccctctcg acatatatgt ctttgctcac
    10921 aagattaaag gcttgaatgt gggtctatat cgttatcttc cagtcgaaca tgagctcgtc
    10981 ctcgagcgac agggagatga ttcgatagct ctgaatgaag cattgaatgg gcagttctgg
    11041 aatgccgcat gcgtgataat gtgggctgct gttccctatc gcagcgagtg gcgctatggc
    11101 agggctgcat ataaacttgt agctctagat acgggccatt catgccaaaa cctctatctt
    11161 gcctgcgaat cgctcggtct cggaacctgt gcgataggcg cttatgacca agagaaactc
    11221 gatgcttatc tcggactcga tggagaagag atgtttgcaa tctatactgc gcccgttggg
    11281 catgtggctc agccataaaa taatagcttt tgtcttgatt agtgctacga accttgtgta
    11341 cgacaatata aacaaggttc gtatcagtat agaagattag cccaaggagg ttttgatcat
    11401 gaccatcgat acagtcaagc tggattttcc aacaaattgc aatgtgataa tcggccagtc
    11461 gcatttcata aaaacagtcg aagacattgc ggagataatg atgaactccg tgcctggagt
    11521 aaagttcggt cttgcctttt gtgaggcttc cggaccatgc ctcgttcgta cagaaggcac
    11581 ggatccatat ctggtcgagg tggctcaaaa gatggccatg tcagtgcgcg cagggcatag
    11641 tttttatctt gtaatcggtc ccggcgcgta tccgctaaat gtgctggacc gcataaagac
    11701 aaaccccgag acctgcagga ttttctgtgc gacggccaat cctgtcgaag tagtgagggc
    11761 acttacggat caaggagccg caatcctcgg cgtggtcgat ggtttttcgc ctaaaggtgt
    11821 agagggtgca gaagataaga ttgcccgaaa ggccatgctg cgagagtttg gctataagtt
    11881 ctgaatattt gccttaatga aagtgcatag acaggatatt tgtataccat gactgaataa
    11941 agccggatcg aggggggtcg aaaggatgac ctggatgaag tgcgctatcg ggctctgaat
    12001 gtgccactga agacatcgaa agcaaccgat ggcaggtcaa aggcttttcc tatcgctcca
    12061 gaatgcgcaa tcacaaaggg agctccggca gaaaaacgat gcgcgcgatc gatgacccaa
    12121 agctcatccg cggtcttaag ggcaagatcc acttcatgtg tacagagcag aagagtcttt
    12181 ccagcctgcc gcacaagacg ttctgcaagc ttgaagactt caatgcgaga aggagcatct
    12241 agaaaggcgg ttggctcgtc gaagatcaat agcgccgtgc tctgcgctac cgcccgggct
    12301 atctgcactt tttgtcgctc tccgtcggag aggctcgaca ccgttcggtt tgcaaaactc
    12361 tgcattccga ccatcgaaag cgcttctgtg acctgttttc tatcctcttc cgtcaggcga
    12421 tgacgcgcat ttgtgtaagg atatcgacca aaggctacca gttcccacac ggtgagaaaa
    12481 ccggtctcga ttcgttcggg gaaaacacag gcaagaagtc gcgctcgttc ttcgatcgaa
    12541 atcctggaaa tatctttgcc catcaataat agagaacccc cacagggggg ctggagtcct
    12601 gctatggttt ttaatagggt agacttgcct gctccatttg ggccgacaag agcgatcaaa
    12661 tatccctgtt ttgccttgaa tttgatatct tgtgccagaa tattgagttt gctgccttgc
    12721 ctccagccaa tagataagtc attggcttcc ataatcgttt taggcatctc tttggaagaa
    12781 taatcaatgg gaggaggtct tagacttttc ccttcatccc ttccagtcaa cgctgtttgt
    12841 ctcgatcctc cttctgtcgt ttcagagcct tcgaatcctt ttgttttctt aaactcaggc
    12901 tcaatgatat cgggaatacc aggaatgtgg cgcatgggat gtcttggatt cattggctga
    12961 cctccatcga gctcttattc ctgcgaaaaa acatcgaaat gatgattgga gaacctatca
    13021 aggccaagat tggatttata ggcaatagcg agccataagt gggaagcgaa gatattatgt
    13081 cggccaagag agcgaaacaa gctcccgtaa gcgcggcaat aggcagtaga atgcgatgat
    13141 cggccttgcg cgatagtagg cgagcagcat ggggagcggc tatacccaag aatgcgatag
    13201 gtccacagaa agcggtgata cttccagcca ggatcgaagc agccaccaag agctttgttc
    13261 ttgaagttcg aaccctaatg ccaattgtcg aagcaaagcg ctcgctcacc agaaaggcgt
    13321 tgagtgcctt tatctcgcgc agggttatga tcaatccaag gcagatagct atcgctaaat
    13381 aaggcagctc atttgttcgc acacctccaa aggagccata ggtccacgaa agatagagtt
    13441 gaactttttg aggagaacca aaatatacaa gcagtgacac aaggctcgat gtaagataac
    13501 ccactagcag acccattatc agcacggtca caacctgttc aaatcttctc gatattagaa
    13561 gaataatgat aagcacggcc gcggacccag tgctggacgc aattataagc agtgtatatc
    13621 ccaggggcgg cagatttcct aagagcgcac tgccttgagt tcctattccc tgtgcgctag
    13681 ccccaagagc tcccagagat cctcctgcga acattagtac cgccactcca atagatgcgc
    13741 ctgctccaat accaagagaa tccggcgctg ccaaaggatt tcgaaaaatg gattgcagga
    13801 tgagccccgc aaccgagagc cctgctcccg ccatagccgc tgttagcatc ttgggcattc
    13861 gaaatacaag gacgatagta gtccattccg gccgcgatgc tttactgctt aaggacttta
    13921 gcacttccaa aagggggatg cgtaccgaac ccaatgctat gtccagcaga gcaagtagca
    13981 gaacagaacc tgcaaggcat gcaacgagca atccctgccg tgcgctatta ggctgattgt
    14041 ctgtcatttc tttattgatt ctattattat agtccttata ggtactcatg cagcagtact
    14101 caggctctat tcagttaatc ttttgtaata attgaactta tggttcggaa gaagctccgg
    14161 ttgaaaaata gcaatcaagt cagaaagaac aagatgagga ttcgttaccg cggactcgaa
    14221 gtaatcgttg cctcctccag gagaacacct gagatcattg ttccaaacct gtcctttctg
    14281 cagaacaggc agtgcagaaa agcgagggtc cataacccgc acatcggaaa gtcgcttcgc
    14341 tccggagaca gggttgagcc atatatccgc ctttagtgct ctttcgaaaa ctgcctccac
    14401 cgaaagattg agagctcccg tgcctttatt gtctgcccaa agataatcgc cacctgcatc
    14461 ctttatgagc cttgccatat agctctgccc acccgagacg ctccatatgc cttggaaagg
    14521 accgtttacc aggacttttg gtcgcttaga ggctcctgat gcgagcgttt tgaggctctc
    14581 ataggctttt atcacctctt ccatgcgttc ttgggctatg ctctccttgt caaagaaagc
    14641 cgcgatgaac accgtccatt gagctcttgc tattggatcc tgttcgttcc aatctccaat
    14701 cagtgcgata ggcagccctg cctcctgcat ctttgggaaa gtatcccatt cgttgccaat
    14761 gccataattg aatatgatat caggctttag catgatcagc cgttctatat cgggcatcca
    14821 attctttgag gtctcgatag tcttgcctgt cgctatctgt gcacgcaggg cgggactata
    14881 tacgtaatcg gcattgtcga cacccacaac tgcatcgagc tctccgatcg cctcgatagc
    14941 agggatgtag gtggtagaat aaagaacgac gcgcttcaca ggcactgcta tggcgagatc
    15001 cgcagcgaca tctcgaggag ctttagatcc tctggggtag agcacatatg tttttgtaga
    15061 agaagctccc ggccagagat tgtatgcctt taatagtttg taaccatttt tttgctctat
    15121 gctgaagctt gcatgtagta tgctggcttt ttgatatgtg gtttgctgag tgcttgttgg
    15181 ctgggcactt aaatgttgtg accttgtttg ctggaggtcg aagaatggaa aaaaaatcag
    15241 aaatagagaa aggaaaatac cggcgattcg tcgcatatcc gcctcctccc accgaagatt
    15301 aaggatggaa atgttcaagg gcaggtttcc tggcttgtcg gctatgcaat ttctcagttc
    15361 tataatgctc aaatctcagc ataagaaaag ctagaaatgc acacgatgcg gttctccttc
    15421 ccagttctgg tggcttttgt tctgcttcta tcaatatctt tccgatcgga agccaatcga
    15481 tcagaagtcg aacaaaagcg tcggaaccag tggatcagcc aaagcgaaca agatcaagca
    15541 tctattgtga tagcgatctt agctctggct gcagcgacat tgtacaacat cgatatcgct
    15601 ggaccgcatc tataccaact cacagtagcg ggggctgcaa cggttttcca ccgttttccc
    15661 ttttccgccg cgcggcgacg ccgcacaaac ggcacccatg agcttggttt attctactac
    15721 aatgggcaga ataagaaaag gtcgcgtctt tgggctgtgt actattatat atgctctacc
    15781 aaaatcgatg catgaaggaa tcattcatcc aagaaagcca tatcttcggg tctagcgacg
    15841 acatggggca cattatcgcc caggatcaca tctatctctt cgcttctttt atgcatgaca
    15901 gccctaagct cagccgaaga aaaattgctt attagcttta ctatttcatt gaccaatacc
    15961 acagaatcgc ggccaaaatc gccttccacc gcaactatgc cagccggcag cagggaattg
    16021 cgctctcgta tcgccgctag agcaccttga tctattgtta aattgccgcg aggttgagaa
    16081 ttcttgagcc atcgaatttt atttttaagt gcgtgggttg cgtcgaaaag tgttccgaga
    16141 ggttcaccac atgcgacgcg cttaattaca ttggactcgc gaccatgagc aataacaacg
    16201 cggcagcctg catcgcgtgc aatggcaacg gcggcaagct ttgtcttcat tcctcctgta
    16261 gaaaattccg aacctcgacc accggcaaac gaaagatgct cttccgatag ctctcgcaca
    16321 taagggatgg gcttggcatg cggattttct cttggatctg aattgtagag cgaatccaca
    16381 tcggagagga taatgagcaa ttccgcatcg atcttgctcg caacataggc cgagagcctg
    16441 tcattgtcgc cgaaggcatt gccgatctct gccgtagaga ccgaatcatt ctcgttgaag
    16501 acaggtattg cccgggcttc caagagagtc tcaactgtct tgcggaggtt gaggtagctc
    16561 gtgcgatcat cccactcatc tcgagtcaca aggagctggg cggctaccag gccaaaaacg
    16621 ccaaacgccc tcctgtattc ttccataagt atgggctgac ctattgcggc acaagcctga
    16681 cgcatgcgca cttcggtgac tctcctagaa aggcccagct cgcgtgcacc catgccgatt
    16741 gcacccgagg tgacaaggat tagctgccta ccggcttgaa cgagctcggc aaactgttct
    16801 gctacattat agatatatgc ggtatcgata attcctttcg aaggatatcc tgagtctttt
    16861 gcgacagccc catcgggcaa atcggccaaa aaagccttgc catcggatcg atatagccga
    16921 gtcaggcggg ccgggctaga ttcaggtctg gaaatggtcg ccgtaccaat tttgactaca
    16981 atacgattag cgcgcgcaag cgctgcgcgc gcagaggctt catccatagt ttcgtggatt
    17041 tgcacggtat tttcctctct caattcgggg gaagttcttt atgcacaaag tggcgttcgc
    17101 ctgaagcata gtcactgacg atatgcccat agccttcgag cagccatttg taggtacaaa
    17161 gtccttcaag tcccattggg cctctggcgt ggatcttgct ggttgaaata ccgacttctg
    17221 cgcccaggcc atagcgatag ccatccgcaa atcgggtgct ggcattgtga tagaccgaag
    17281 cggaatccac ttctttgagg aagcgatttg ccgcttcgga agaagcacag actatcgcat
    17341 cagtgtggct gctcccataa tggtttatgt gcgcaattgc ctcatcgagg gaatatacaa
    17401 tcttgacagc cattcgcatg tccaggtatt cgacagccca atctttatcg gtctttacag
    17461 aatagtgcag attccgggtt tgcccgggat ccttatcgag tatctgggct gctttttcgc
    17521 agacatcgag catcactccg gctttggaaa gagcttgcaa aagcgccata aggctgcgat
    17581 gcgcaaatgc ggcatgcaca agaagtactt ccgccgcatt gcaggcagct ggatactgta
    17641 tcttcgagtc caaaacgatt ttgatagcca tcaccgggtc tgcatcttcg tgcacataga
    17701 catggcagat accatccgaa tgcccaagca ccggtattcg gctttcagcc tttatctttg
    17761 aaacgaattc cttggaaccg cgaggaatga ccaaatcgac atatttatcg aatgctagca
    17821 gttcgcccac ttcttcgcgt gtttccagga gagtgagcca gtccgaagga aggccttttt
    17881 cttcgccggc cctggcaatt atagtggcca gaaccttgtt gctttcattt gcctcgctgc
    17941 cgcctttcag cacgatggcg tttccactct tcacggccaa tgaagccatc tgcacaaggg
    18001 catcggggcg gctttcgaag atcatcgcta tgagtccaat cgggcagcta acccttttca
    18061 atatcaggcc atcatccaaa aggcgcgctt ccaaaactct tcctgtagga tcgggcaatg
    18121 caagaatatc ccgaatgccc aaaagaacgt catccagttt ttttttgtcg aattcaaggc
    18181 gccttaaaag cggtatagct aatccgtttt gcctggtggc ggtcaggtcc aattgatttg
    18241 catagaaaat cctatcgcga ttttctcgca agccttgtgc aatgcaggca agcgcagaat
    18301 tcttgacttc tgtttctaag gagagcattc tagaagcggc cagacttgcc cgctcgagtc
    18361 tttgctgcat attcattcat catactatac aaaaaatcaa tgaaatcaaa aagaggtgga
    18421 aatgtattat atttgacaag atgaaccagg tggaattgaa taattcctcg cgaagattga
    18481 acatctaaat tgcaaaggag accgagaaat ggcgcaaaga acaattggat tcattggact
    18541 gggtgtgatg ggggctccca tggccggaca tattcgcgct tccggcgaaa ggcttttggt
    18601 ctattcgcgt acgaaaagca aggcacggct cgtattagac gcaggggccg aatggcgaga
    18661 ttctcccaaa cgaattgctc aggaatgcga tgtgatcttt actatggttg gctatccaaa
    18721 agatgtggaa gagatatact tcggctcgga tggccttatt gagaatgcta gatctggcac
    18781 tattctcgtc gatttcacca catcgagacc tgatcttgcg gttcgaatct acgaatcggc
    18841 aaaagttcgc ggactgagcg caatcgatgc gcctgtttct ggtggggata tcggcgcaaa
    18901 gaatgctact ttgaccatca tggtcggcgg cgaggaagag acattccaga aagtaaaacc
    18961 cctccttgaa atagtgggca agaccgttat cctgcaaggc ggtcctggtg ctggtcagca
    19021 tacaaaaatg gccaaccaaa tagtcgtggc aggcaacctt cttggcacag tagaggctct
    19081 tatctatgca aaaaatgcag gcctcgatcc aaagcatgcg ctcttatcga ttgtgaatgg
    19141 cgctgcacaa agctggcagc tctctaacaa tgcaccacga atgcttgaga gaaatttcga
    19201 tccaggcttc tatagtaagc attttctcaa ggatctgcgc atagctttgg attccgctca
    19261 tgcaatgcat gtagaattgc caatgttgac tctttccgaa cgcctttttg ccaagatggt
    19321 gaacgacgga atgggagagc tgggcacgca ggcaatatat ctgctctatg agcgcggatt
    19381 agcataaaaa tgcaatattg cctttatgat ttcttcgatg tcggcgataa tagattgatt
    19441 tttatgaatt ttatgccgat atagcaacta ttgtttaagc tccaggctgt acattagaat
    19501 aaataattgc cccaaaaggg gtgaaacatc gacccaaagg gtcgaaacag gcagtcatat
    19561 aaaaaatgag tcaaagagaa tcagaagccc tacaggctct tgcaagggat gcttcgttct
    19621 ggcgacacat ctacgaaacc cagcaagccg gcatattgat tcaggtcaat ggaataattc
    19681 aggacctgaa tgagactgct gtcagaatgt taggaggacg gtccgcttcc gaattcatcg
    19741 gcaaaccttc tattgaaaca ctgatatgcc ccgagtatta cgattgcatt ctcgatagaa
    19801 cgcgccaact gcgaaatggt cgtccttcgg ttccacccat agatatcaga gtgcgacagc
    19861 taaatggagc ggaacttgat gtactaatat cggcaacttc atggatcgaa aagaacgata
    19921 caataatcga gactatgctg atcgatattg gaccattaaa gcagcgcgaa aggctgctag
    19981 aggcgatctc tgacttggca tctatgccgt taaagaaaga attcttctcg gatgtaaacc
    20041 ttgctatatc gctcatcctg gattctatgc ataagctata tgcggaacct cacaactgcg
    20101 gcttcatgag ctttgtgcag catgaaaata gttttgttga ggaagtttgg ggagcctcaa
    20161 gtgaatcccc tcagcttgta tcctcagatc caatcgataa tgttacgctg aggtggatta
    20221 tcgagaattc aaggattatt acaaaatgcg aatcatgcag agacagattg cataagatgc
    20281 ccatcaaaga agatcaatcc tggcagatcc tttttatgcc gattatcccg gaggacaaac
    20341 tggaaggctg tgcgttttgg attttctaca cggcaataga cactgataaa atcgaagcag
    20401 atgaagagcg acgaaatgac tttattcaag ctgccttgat agctttgaga acatttctca
    20461 tacagcgcga taataaccgg aagacctcgg agctctccat cctcaacaaa atagctcttg
    20521 aggttggcag gaagacgagc cttcaggagg ttgcagacac tacactcagc attctcgaaa
    20581 gagaaaagaa ttggcagcct tcggtaataa gattcaaatc cagattcggc gagactcttg
    20641 agacgatttc gtacaggagt tcgcttgtta agaacgtcga agaattgcaa aagaggatgc
    20701 atctctttga tggattgatc gaccgtcctg gtctgggtat gacaggctat gtcattgaga
    20761 gcggaaagcc tatacggtgt atggatttac cctctgatcc gcattatgtt cagaccgatc
    20821 tgaatatcaa gtatggcata tatgctccca tatcgatcga aggcaaggtg gagggagcaa
    20881 taggagtaga gagcaaagat tacgctttcg atgattctga cctgcgcctc ctgcagtcta
    20941 tgagcgagat tgtcggtatg gctataaggt ctgtaaggct catagaagtc ctccaagaac
    21001 gtctgcattg gcttgagata ctacatagaa taaatgaaca gattggaatc gagacggaac
    21061 catacaagct ctacggattc cttgtcgata gcgcattcga agcaacaaaa gctgaatccg
    21121 ttgccctact aatctataat ccacagacag acatgttgga aaaggttgca gccaggtctt
    21181 ggctcgatag ggtctttgaa ataccgctaa agccagacga gagcataagc ggagccatat
    21241 tccaaagtgg aaagatgcgt ttttcgcctg aaattagaga agatcctctt ctcatatcga
    21301 ggaacctttc gttaatcccg ccaggatgcg ctaatatagg attgcccgta acatatgaag
    21361 aaagaatatt gggagtcttt catatttcga tcaaggcgcc tttgtccttt accaaggagc
    21421 tcgtcgaatt tgctgaaatg ttcggatcat actcaggtat tgtcattggg cgtatacaac
    21481 atgtccgagc tctgaaggaa gcggagaagg aaatcaagac agcttacgat gagaccttga
    21541 agggctgggc tcgcgcgatc ggctataggg ataatgaaac cttccagcat acactgcggg
    21601 taacagaaat agcccttgca ataggcgaag ctctttccct cgatgagaag acactcgaaa
    21661 acctaagacg tggcgccata cttcacgatg tagggaaaat cggcatacca gacaacattc
    21721 tacgaaaacc caaggcattc accaaggaag aagaagcaat catgcgtacc catacatctt
    21781 ttgcgtacga gctcctgcgg cctatagact tcttgaaaga tgcgcttagc gtaccatatt
    21841 gccatcacga gcgctgggat ggcaaaggat atccgcaggg tctaaaaggc gataatattc
    21901 ctttacttgc aaggatattc tcagtagcag acgtctacga tgcaatgaca agcgaaaggc
    21961 cttatcgtca tgcttggact cgcgaggcgg ctttagaata tatccgatcg caatcaggga
    22021 aacatttcga tccccggatc gtcgatgttt ttttgggaat cgtagacaag ataaaggatt
    22081 gagcaagaat atagcggagc tccgtatact gatacgaacc ttgtttatat tgtcgtacac
    22141 aaggtttgta gcactaataa atatgttacc tagagctggc gaccagattt gagcttctct
    22201 tcaaagcgct cgagttcctt cctagatagc tcgctagtct ggtcttcttt gctcgattcc
    22261 acgcattttg tcgcgacatc ttctaaggat tcggccttca ttagtctttg tcgaagctcc
    22321 ctcatctcag gcccagagaa tcgcacagcc tctaaagtat atcgttcaaa actcgccgtt
    22381 gcagcaggcg caacttttct ggttatctcg agaatgacct ttgcatattc gcggatttca
    22441 cgctgtgcat gggagtctag ccttaacatg agaaaacgaa aaagattatg cagatccatc
    22501 tgccaataca attcggtata gagagaaagg ggcagagcta tgcgagccac ttcgcgggca
    22561 aggcccatat cgatcaactc cctatagcgc tcatatgaaa agagagaccc ttccttgaga
    22621 atatcctgga tcctcgatgc tgtttgtttg tcgaaaggca tgtcggaacg gccttgcttg
    22681 ttgtcttcgc tctgtggagc caaggcgtct tcatatggaa gaaaaaactc atctcgcata
    22741 atggaatagc gaccggaaat ctcgttgacg cgcgcggtac ggtggcgaat ccactgtcgt
    22801 gccacaaaaa taggtagctt tacatgaaag gtgagcacaa cctgctcgaa tgggcttgta
    22861 tgctcatgcc gccataggta atctatgagc gccgcatctt cacgataact cttggtgccc
    22921 tcaccatagg agactcgggc ggcttgcgca atgcgctggt ctccgccaag atagtcgaca
    22981 agtcggacaa accctttgtc gagaacgggg aattctttgt ctagaatcgc ttctgcttcc
    23041 ggaactatac agtgtgccat agatctattg tagcacatat ccgatttgtt gtattgagac
    23101 ctggaaagaa attgcccggg ctggcaatgg atatactagc aatggatatg ccaaacattg
    23161 ctggactatg atagcataaa ccccatgaat tcgaaggtag agctaatact tgatagaact
    23221 tccggccaca ttttgaagat cttctttaag gctaaagagt cgatagagtg cgatgagccg
    23281 ctcctggagc tgggggagat tgagatcaag agtctttgcg atgcgctggg caatgagtgc
    23341 caaaaggcgg aagaagccgc agggtttagc ttaggcagtg ccacagaaaa gctttcttcc
    23401 gcgcgaaaga gattcgtgct ggtcaatggc tggcaatcct ggtcatttgc cggggaactg
    23461 aagggaaggg agaggccgcg ccgcgccctg ttgcgccgag atctaaacct gtttgtcgac
    23521 catccagcgg agcgggcgct gcgcctggcg gcgctgcgcc gcccggcgca gcgcccgcac
    23581 accctttgcc agcgggcacg gcgcttattc cataggcgcg acattatctc gcacttcttg
    23641 ctcggcctca gagccgacca gctaagactt gtgctcgtgt cagacaatgt ggagcgctgg
    23701 aacagccctc gctcgacaga tgccgaccgc gcctatctgc gagatctttc aagttgctcc
    23761 agaaatgaaa gcccttgccc agactatttg cctcccgtca gttttatact tcgaggcgat
    23821 aagattagtg tttttgttta tgtggcgggt ggctcctttg ctgccggcga gacaatcgcg
    23881 cggctttgcg tgtttatcgc gccagactac ttttcgctta aagataagct tggctcatat
    23941 tggggctccg atcgtcgttt tgacgattcg agttggctct atgagccaaa atctgtttct
    24001 catggcatga taggtggcta tgcatcatgg tacaatcact acactgccat cgatgagcag
    24061 attatcggga atgacctcga tgccataaag gccaatgaca attttgtaaa cagctatttt
    24121 ctgcggcgag gcctgcccgc cgtattccag atcgacgatg gttgggagat ccaggtaggg
    24181 gactggcaag ccgatcctcg caaatttccg aatggcatgg aaaaaatggc ccggcgaatt
    24241 gccgaaaagg gccttgtgcc aggcatctgg ctcgcgcctt ttcttcttat gcccaattcg
    24301 gccacggcaa aagcccatcc cgaatggctg cttcgcgatg ctagagacaa gcctgtcaag
    24361 gcaggctgga accctaattg gggcggcgat gtatgggctc tagatatttc ccaatgtgat
    24421 gtcgaagatt acctcttatc tttgatcgat acgattgtaa atgtctgggg ttatcgctat
    24481 ttgaagttgg attttctata tgcgggtctc atgcaagggg tcttttccgg tcgaaaagga
    24541 ggagcttggc agcactatgc ccggattatg gcgcatatcc tggaattttc cagagcgtcg
    24601 aatggcgagc ccgtagcttt tctctcctgc ggcgcaccca tagaatccac cgcgcctttt
    24661 atgcctctaa tgcgctcagg ggcggatacc cgagagcatt gggaatggcc acagctaagg
    24721 ctcataggtc atcaaggtcg gcctgcggca aaagtcaacc tggaagatag catcggacgc
    24781 gcaattctgg acaagagtct tctactatgt gatccagatg tcatcttttg cagaacagac
    24841 ctgaccagcc tcaaggatac agaaaaattc cttatcgcaa tggttgctgc gatgttcggt
    24901 tcccagctca tgacctccga tgatcccgcg ctctttacaa atcatgctaa aatagacaat
    24961 aaccctgata agctctcaga ggccgcattc acaagagagt tgatcgaatg gtacaagcat
    25021 atcgagggca aggagttcgg catagagaga aatccaggcc gaacacgcgg actctatgat
    25081 ttcttcagca gagatggatc tatatttggt agcatcaacc tttctgacag agagcaattg
    25141 atcttcgccg aattatcaga gccttccgaa ttgactcgat tatccgaatc gtctgaatca
    25201 tttgaatccg tagcgccaga tctgtcttcg aagttcccaa tgccacgcca ttcgatgatt
    25261 atctttggcc tatagtgttt tccatttcga atgcttaaga cattatagaa gaagcgcaag
    25321 accattcatc ataaggaccg aatatttttc agccgatcgc gcgagaagcg aggatcggga
    25381 gagcgataaa attcccaatc attccgccaa ggctcgaaaa gaaaaagact agcagaatat
    25441 gggttatacg attcctataa aagcctttaa ggctcgtcac atcgtcggct agattctccg
    25501 catcgtgcac cgtgggctta cgaaaaaagg cttctattat ggctgcaaac aatcctattg
    25561 caagtaaggg attcattgtt gccagcggcg ccaataagaa tgaggagaga attgtaaccg
    25621 gatgtgccat gcataggagt gaaccaagcg ctgccaaaga accattcagg agcacccagc
    25681 gttcaatcat tgccaggctc gcctggcctc cggaacgaaa aaaaccgatg gttatcagcg
    25741 caattatgag gatgggaata agccagccag cagacttggc aaaccagccc ttcttcggaa
    25801 gggtttcgat atcggagaca tcagcctcga tttcgcctcg cgatagcttt tctaaccaag
    25861 cttctatgcc gttcatgtgg ccagcgccga ccacagcgac cactttctgc tctgtggtca
    25921 gaaatatctt tgtggcaaga tagcgatctc gttcatctat gagtacttcc ttgaccgacg
    25981 gcatataact ggcgagttca ttcatcatct gctcgagttc gctgctctct ttgagttttt
    26041 caagatcttc ttcgctcagc ttctcacgcg agaaagccga ttctatcagg ctggccagga
    26101 gttttgactt gttccagaga ttggattttc cccacgctcg cttgagagtt atctgaattt
    26161 ctcgatctat ggcggaccag ggaataccca tttcctcggc ggtttggatt gccgcaagca
    26221 tttcctctcc aggcttagtt ccaagatcca acccgaggcg cttctgaaat ccggcgagtg
    26281 ccagatttgc cagcaataga aagccgtttc cctgctttag tacttttatg atgtcgagtt
    26341 tttcccattg gctctcttgg ctgatcgatt gatagcgccc catgtcgatc tctacgcaaa
    26401 cgcgctgcgg ttgttctgct atgatagtcg ctttagcttc ttcgatactc tccttcgata
    26461 tatgagccgt accaacgagt atgatggttc tttcaccaag ttgaatggtc ctgacagtct
    26521 gcgacataat atgtcctaat ttataaattg tggatataag cctcgctcac tccatccggc
    26581 aagcgggagc ccttttcctt tttgtgtgat tatagagatt atgagcagct gcagcaattg
    26641 gcggtcgaaa gccgatccca ataggcctcg aatggcagcc tcggtttcgg aaagggcgga
    26701 tatgattcgc ctgcaatctt ctggctcata attgccaagg gcatttcgga gctttttctg
    26761 aacagttttg gagcgagctt gttctcggat gcatacatct tcgaagcgct cgccatcggc
    26821 aactgcttct cctatgcgcg ccagtctgcg aaaactccaa actagagcgg ctattatctg
    26881 attggcatcg ccgcgccttg agtccagaac gctattgagc acttcgagag aaagagataa
    26941 atctccctgc cctatcttat cgaagagagt aaaggcgtct tccggcttgg agcgcagaat
    27001 cgccgcctcg acatccgaag tgccaagcat gcttcctgga gggaaactcg cgcttagtac
    27061 aaggcaggcc gctgcaagag agttggtgtc atgcggaaca agctcaagga aggattcaag
    27121 ggcctcttcg tcgatagaaa gctgggattt gcctagttcc cgccgtatcc atccggttag
    27181 ttcattttcg aagagctcga agaaggtttt tcgacacgaa ggcggcacga ctttttctat
    27241 ggcttttgag accgaatagt tttctgtttc gagcagcagc agcgcttgct ccgccggact
    27301 gccgcaatat ttgaccattg tatctatctc tggcttcaga ctgatctgat ccgcatttcg
    27361 gaattcgatt atgacccatg gagaaaagag agaactattc tgcagaagct ctatagcttc
    27421 ggagggcgag ctatcgccag catacattct atgaacttca ggttcaccct ccccaagccg
    27481 ccttgcctta tctatcaaat cggctataaa agcccctttt agtcccactt cgggccctgc
    27541 gagtatccaa acaggaggaa tgggatattt tgccatagga tcaatagctc ctgtatatac
    27601 cgcgaagtct gccaagtatc ttcagatcct gagtataaat tggagcgtag cctggatttt
    27661 ctgcctgtag cttgtagcgg ttcctttcga tatagaatcg cttgagggtt acgttttcat
    27721 ctagaagagc tacaatgata tcgccattcc ttgccgtctc gctctgctct attatggcga
    27781 tatcccccga aagaattcca gcgcctacca tgctgtcgcc atttactcga agtgcaaagt
    27841 gaggaatatg attacctacg aattcagccg gaatggagat ggttttttcg aagttttcgt
    27901 ctgcaaagat gggcttaccg gccgcaattt cgcctaagag cggaattttc accacagccg
    27961 tattgtcttc ggggggcgag accaactcta ttgcgcgaga gcgtttctct gtggtcttga
    28021 tgtaaccttt tttctcgaga gcctttatgt ggtcgtatgc gcctttgacc gacattgaga
    28081 aggctcgtgc cgtttctctt accgttggcg gatagttgtt tttttgaata tactcccgga
    28141 tgaagctgag gattagcttc tgtcgatcgg taagatcttt caatgcagac tcctgtatct
    28201 ttgtatagta tacaacacag aactgcaaaa cacgcaagcc ccatatttct atgggctttc
    28261 tatgggctct tctttatcca agttcttcgt tgaagccgga ttcgaaaagt tccgctatgg
    28321 cctttacggc ctcttgttca tcggcgccat cggcgattat cttgattttg gcgccaaagc
    28381 tcgcggcgag ggtaattatg cccattatgg atttagcatt gatctgattt ccttcttttt
    28441 cgaggtatat gcgagatgcg aatttaattg cagtctgggc aataagggca gaaggacggg
    28501 catggatacc ggcacgattc ttaattgttg tctctagctc taccataagg gctccaagaa
    28561 gaagattcaa ggccaatagc agtcataata cttcgaatgc aggtttgcat tcaataagcc
    28621 cgacagtaca tgagtaaaat gatctaataa tcgtctcggg agaaaaacat ggtgcgggcg
    28681 ctctcgctct cgagccaccg caggatattc tggttgaatt ctcttgcgct gtgatatccc
    28741 atttttttga gtcgctcgtt cattgcggcc gtttcgatta tgatggctaa attgcggccg
    28801 ggcttaacgg gaatttctag cttcggaatc tttacgccga gtatttccat cgtcaattca
    28861 tctgtgccga ttcgatcata gactttctta ggatcccaat cttcgatttg tatgacgagc
    28921 tgtatctgtt tcttatctcg aatggcgcct actccgaata gatgcgtcac attgattata
    28981 ccaagacctc gtatctccat gtggtgccct atgaccttgt ttgtcccctg gcccatgagc
    29041 aggctgccat taaggcaatg aatctccaca acatcgtctg cgacgagcct atggcctctt
    29101 tcgatcaatg acagggccgt ctcgctcttt cctactccag attcacctgt cagcaatatt
    29161 cctatgccga acacttcgac aagcacgcca tgcatgatca ttttaggtgc aaagatgtcg
    29221 tgaagaatcc gaataatccg aatagataat tccatcgaat tcagttcggt tatgagcaca
    29281 gggcagccta ctgcttcggc atttgagaca aagagatcgg tcggtctaat gccgtttgta
    29341 aaaatgcagc aagggatttg gtacgaaaag agcttttcgt atatttgagc cgccaccccg
    29401 tcttcctcaa ttttgtggag ataggcactt tcgcctcgac cgaatatttg cagcctctcc
    29461 caggcaaatg aatcgtagaa tcccgccaga gccaggcctg gcctattcag atctggtaca
    29521 ctgatttctc tgctgagccc ggctcgaccc gctgtgcatt ggagcttgag atcgttgtgt
    29581 tcctttagtt cgaggcttag aagatcgaga acggtaaaat tgacaggttc tttttctttc
    29641 acacgattat tctacatcat cctgcaaaag tggtaaatct aataaaaaac ggcggtcttc
    29701 agccggctgc aatggcagtc tcgaaaggcc tcgtttttga gctgtttttt ggcctccgtt
    29761 tgcatgaaga accgccgccg tataagctgc tctcacttgt tctttttctg aattttttct
    29821 ttttccttta ctattttatg atctagcttg tcgataagca catcgatgcc tgcatttagt
    29881 tctggtgaag taacaacgag gtgcgcctgc ttaccccatc gaaagttcgc ggttacctcc
    29941 aatcggaacc gtccggcgtc tttagtaaag acgaaaagaa gatcgacgat caagtccttt
    30001 gcgtaatcga tccggctgat tttagtattc agatactctc tactctgttc gctaagatcg
    30061 aagtgcacag aacggacgtc gatattcata atgacctcct caaagattat ttgtgccttg
    30121 gccaggccaa gcgcatgaga atcatcggct cggtctactt tccgagctcg ccacgatatt
    30181 tggccaccgt ccggcgagca attttcattc cccgccgggc gagaatctca gaaataatac
    30241 gatcggagat tttttcgcct tttgcctcga gttcttgaat tatctgagaa atctcggcct
    30301 ttactgcctc cttggagatg tttttgccct tttcttgatt tatagcattg gtgaaaaaac
    30361 ggcgcatttc gaaagtgccc cagtcagttt gcacatattt gccattggcg acgcgtgaaa
    30421 ccgtcgcctc atgaactcct atcttttctg cgatttcttt taatgtaaga ggctttaacg
    30481 cttttggtcc tgccacaaag aaatctctct gggtttcggc tattgcatag gcggtcttta
    30541 gcaaggtttc attgcgcttt tttatgctat ttatgaaaaa gcgcgcattt tcgacctttt
    30601 ctcgcgcaaa tttctcggct tcaccttttg ctggttgaga agcaagttcg ctatatagtc
    30661 ggttgattcc aagcttagga acgaaatcat cattcagtcg cacttttatc tcgccatcct
    30721 ccagccacat cgcgatatcg ggcacaatat agcggggtct ggctttggag aacgagcgac
    30781 cggggaaggg ttccagggtt cggatgaatt cgactatgcg atctatttga cgatcatcga
    30841 gcccgagtgc ttttttgatc gaacttcgat cgcgaagtct gaggtcttcg aaatgatcct
    30901 taatgacatc gatagttttt ggaggagcag agggagaaat ttctgtttgt actatgagac
    30961 tttcacgaaa gtcggaagtg caggtgcccg gaggatcgag cctttgaagg agatatagca
    31021 agcgattcag agtttccgga tcgcaatcat tgcatagttc cgaaggcggc agaatatgaa
    31081 aaccattttc atcgatattt tggatgatcc tttcagcgat tttgcgctct tgttcattga
    31141 gaggtaagag cccgagctgg ctgagcaaat gttcctggag tgactcttcg gttgctattg
    31201 tttgttctat tatggcgtta tggtcgatgt catcggtatt gaatccgaat atatcgtccg
    31261 agtactcgcc ttcattgagg ctgttctctt tttctgccat attatcgatg tgcgattcca
    31321 tgaaatcgag cgatatctcg ctcgagccgg taagcagttc gagtgccgga ttttcttgaa
    31381 gttcctgatt gatctgttca gtcagctctt gcagagggag ggtgagcaga tgtattgatt
    31441 gtaccatcct ggggttgagc ctcaagcgtt gctgctgtac gagaaccggt ctttgggatt
    31501 gcaatgcata ctctccttat attaaatgta cgacactttc ttcgaatgtc aaggctgagt
    31561 aggtttcacg cgctctgctg aattttgaca tctaatgaaa gaatattgcg aatgattgca
    31621 gcatttgtat tgacgatcat gatttttaat tagtattttc attaagaaag acaataatat
    31681 tattttttat tagcagggtg aagaatgtct aagcacaagc atgaacaaga acaacaatcg
    31741 gaaatcagaa agcaggagaa ccttcaagat caaaacgcgc ggaatgcgcc ttctgctcag
    31801 gaatccacaa tggaacatgg agccgacaag caacagttga atcagtctac gagcgatctg
    31861 gagtctcaac tggcggcagc caatgaggag atagcgcgcc tcaaggctca gctggatgag
    31921 ttgaacgaca agtatctgcg cattcttgcc gagcaagtca attttagaaa acgggttgtc
    31981 aaggaaaagg aagaatttca gcaatatgcg gtctcgatgc ttctaaatga tcttattccc
    32041 gtgctcgatg actttgatcg atctttggat gcagttgcgc agaatcataa cgatgtcgaa
    32101 aaagtcatcg aaggtattcg ccttattcag aaaaggctct atgatactct gtccaagaaa
    32161 tatggtctca agcaatataa ggccgaaggg accgtctttg atccgcattt gcacgaagcg
    32221 atgttctccg agaaaggtga tgtgcacgaa ccaagggtta cccaggagtt tatgcccgga
    32281 tataagctgc acgatcgagt tattcgaaca gctaaggtaa aggtcatgat gccggctaat
    32341 cctgcggagg ccccctccag agcaacagag gtgtcaggag agactcaaga ttcttcattc
    32401 tttgagagta cggaggagaa cagcgctcta ggataagagc tcgatttgct ttgtggtgga
    32461 tagttttgta acttttagat agacaagaaa gaaggagaaa gaaatatggg aagaattatc
    32521 ggcatcgatt tgggcactac caattcctgt gtcgcagtga tggagggagg agagcctatt
    32581 gtaattgcca attctgaagg acaaaggaca acgccctcgg ttgtagcctt caccgcaaaa
    32641 ggtgagaggc tcgtaggcca gccagccaag aaccaaatga tcacaaatgc agagaacact
    32701 gtattctcga taaagagatt catgggtcgt cgctatagcg aagtattgaa tgaaatcaaa
    32761 cttgttccat tcaaggtcat agataatggc aacgatgtgc gcatcgaggc tggcggtaag
    32821 ctctacagcc ctcaggagat tagcgccttt attcttcaga aaatgaaaaa gaccgcagaa
    32881 gactacctag gcgagccggt tacggaagcc gtaatcacgg ttcctgcgta cttcaacgac
    32941 tcacagcgac aagctaccaa agatgctggc cgaattgccg gtctcgaggt aaagcgaatc
    33001 atcaatgaac ctactgcagc aagccttgct tatggtttca acaaggatca gaagaaagaa
    33061 aagaccatag cggtatatga cctcggtggt ggtaccttcg atatctctat actggagctt
    33121 ggagaaggcg tattcgaagt caagtcgacc aatggcgaca ctcacctagg cggtgacgat
    33181 ttcgaccgca gaatcgtgaa ctggctggta gaggaattca agaatgatac gggcatcgat
    33241 ctttcgcatg atcgcatggc tgttcagcgg ctacgcgaag catcggaaaa ggcaaagatc
    33301 gagctctcca atatgcagca gaccgagata aatctgccct ttataacagc cgatgcgaac
    33361 gggccaaagc atttgcagaa aagccttacg agggctagat tcgaacagat gtgcatggac
    33421 ctcatagagc gatcccgcga gccttgtatt aaggcgcttg cggacgcagg gctttcggca
    33481 tctcagatcg atgaggtaat cctggtaggt ggaatgaccc gtatgcctcg cgttcagcag
    33541 atggttcgcg aaatattcgg caaagagcct tccaagggag tcaacccaga tgaagcggtt
    33601 gccattggtg cggctatcca gggaggtata ctcggtggag atgtaaagga tgtgcttctc
    33661 ctcgacgtta cgccgctttc tcttggtatc gagacactgg gcggcgtatt tacccgtctc
    33721 attccgcgca atacgacgat tccatgccgc aagagccaga tattctctac ggcagcggat
    33781 ggacaaacag ccgtatccat tcatgtattg cagggcgagc gcgagatggc ggcacaaaac
    33841 cgaacacttg gaagatttga cctagttgga attcctcccg cgcctcgtgg tgtgccacag
    33901 atcgaggtga ccttcgatat cgacgcaaat ggtatcgtgc atgtgtcagc aaaagatctt
    33961 ggcaccggaa aagagcaaaa gatccgaatc gaagcatcga gcggtctcaa tgaggcggag
    34021 atcgaaaaga tggtcaagga agccgaagcc catgccgagg aagacaggcg tgagcgggaa
    34081 cgcatcgatg cgcgtaatga ggctgattct ctcatctata gcacggaaaa atcgctaaag
    34141 gaagttggcg acaaaataag cgcaagcgaa aagtccgcgg tcgaaaatgc cgtaaaagag
    34201 ctcaaggatg taatggaagg caaggatact gagctaatca agcagaaaac cgaggccttg
    34261 aagcaagcat cctataagct gtccgaagaa ctgtaccgaa gtgcttcggc ccagggcgcc
    34321 gcaagcgaag catccggtag cgccacagga gaaagcgcct ccgatggttc cgctggctca
    34381 agcgataatg cgcagaatgc cgacgatgtc gactacaaag tggtcgacga cgacaagtaa
    34441 cctagagagc atatcgaggt ggctgggccg cggttcagcc acctttttat caaaggattt
    34501 tcgaagtggc caagcgtgat tattacgaag tgctcggtgt gccaaagacc gcaacaaaag
    34561 acgagatcaa gaaggcctat agaaaactcg ctattcagta tcacccggac aagaaccctg
    34621 ggaacaagac cgcggaggaa aaattcaagg aagccacgga ggcatacgag gttctgggag
    34681 acgaccagaa acgccaggcc tatgatcagt tcggctttgc tggtgtcgag ggaatggcgg
    34741 gagctcaaca ggactattct agcgtatttc gcgacttcga ggacattttc agcggatttg
    34801 gcgatttttc atcgtttttt ggttcgattt ttggcgaagg gtcgcagcgt cgtggagaaa
    34861 cacgtacaaa tcacggtgct aatttaagat acgacataga acttcccctt gagaaggcgg
    34921 tttttggaac caccgtagaa atatcgtaca acagagatga tgtctgcaag acctgcgggg
    34981 gcagtggttc gcgcgacaat cttggcagaa aagtttgttc gacctgcaag gggacagggc
    35041 aggttcgcag aagttcgggt ttttttgcca tttctcaacc ctgcccagca tgtcatggag
    35101 agggcacggt aatagagaat ccttgtcagg attgcgccgg aagtggagta gtcaagaaaa
    35161 aacagaaaat tagagtcacc atacctcccg gtgttgaaga tggcaaaagg gttactgttc
    35221 ccggccaggg gaacgctggt cctaatggag gacctccagg agatctccat gtctttatcc
    35281 atgtgcggcc tcacgaaacg ttcgaacgcc aggggaatga cctttattgc gccgtctcga
    35341 tagattttgt aaccgctgca ctcggcggcg aagtggttat cgaaacgctg gaagggcgga
    35401 agatatccgt tcagataccg gccggaacgc agaatgctaa gttgctccgc ataaaaggcg
    35461 agggtgttcc agtttcgggt ggcaaacgcg gcgatctcta catcaaagtc tttgtcaagg
    35521 ttccttcccg gctcaccaaa cgagggcgcg agctcctgga agagcttaag aaaatcgagg
    35581 gtgaaacctt gcagcccgag ctcgtcaagc tgaaagacat accaaggtaa tataagaagg
    35641 ctgctcggaa aatcgggcag ccttcttatt atttattatg gaatgatctc cactattcgg
    35701 tagcctgcct cggtcaccgc agcccgcaga gcctggtcat ccagttgttc accctccaca
    35761 attgcctggc gttggagaag gtctaccttt gcgcttctga ctccgggcac tgcttcgagc
    35821 gcagactgca catgcattac gcaatggccg caggtcatac cttcgatttt aagcaatttc
    35881 ttcattgtct atcctccctt tctctagagc cctagagtcc atcgatctga ttttctatat
    35941 ctcctgagcc gaagtgcatt agttgcgaca gaaacagagc tcaaggacat ggcggctgcc
    36001 gcaaaaattg gcgaaagcaa aagcccgcca aaagcatata acaagcctgg acactatcga
    36061 ccgaaatatt ggtcacggca tttagcttgt tcaccgctag atcatttgcg gcaacgcaag
    36121 cggcgcatgt catacctttt attgcaaaag tcttgcgatc agccatggag gactccatat
    36181 aaatccatca agacaaatat atagtaattt tagcgaagtg ttgtagactt tgacgagaaa
    36241 tcgaatctaa ccacagtgcc cttgtcaagc tcacttatta tttcgagctt tccaccatgt
    36301 cgctctatta ttttctttgt aatggcgaag tccacgttaa tctggcctgc ggattgccca
    36361 ttctcggtgt tagagaatgg ctttagtaga gaatagacct cttccggaga tattcctatg
    36421 ccattatctt gcacaaagat gcaggattct ccgcatttgc ctatttctat catggcagtc
    36481 agagtctcag gacaattctg gatagcattt tcaagaacat tgcgtaaggc aatcttcaag
    36541 agattgccat ctccgtgtac tttgaggtca ggcataatgg taatctgata gcgccttttg
    36601 ggattgcgtt tgattatttc cgaggtaatt tcctgagcag cgatactgaa atcgaattcc
    36661 tcatccgcaa gctgtattgt gtctatcgaa agaaaaatcg aaagggcttc cgttagtctg
    36721 gtcatgcttt cggttgtttt tctgatgttc gttatatagc tcatgatttc gctatcagac
    36781 ttattctttg ctagggcttc cagagatttg ctcaaggatt caatgttgta aagtggagca
    36841 atcaagttat aagagattgc cgatgcaatt gcgcgcaact catcatttgt atctgaaagg
    36901 cactttgtct gttcttccac tttttgtgca agttctcgat tgagattgtt gacctgttcg
    36961 agcatcgttt caagattggt tacatcgcgc attacataga caaagagcat tggctgatcc
    37021 ggcagggcaa tctggatctc tgttgaatca aatctcaggg tctgtccctg tggaccggca
    37081 atctcgatcc tctgaacctc ggtttgagcc gttttggtgt ccatatgggg caagcgcaga
    37141 tccttgggaa gggtatctcc aaagtggtct ctgaaggaat ggtttgccag tcgaatttcg
    37201 agattctcgt cgattgctac caccggaaaa tcgatacgtt cgagtatggt gctcagaagt
    37261 gcctcgttgc gttcggtgcg cttgagcagt tcgaatttcg actgaaaagt ggcaagcgct
    37321 cgtgtggaga aaatcacgta tgagcctgcc aatagcgcta ttatggcaac gaagatgaat
    37381 atgataaaaa caacaatcgt cggtattagc gaagcaaacg cttccgcata gtcgagcttt
    37441 gaaagaatta tccagtctag atttgggatt ttttctgcat aggccagcac ttgctttccg
    37501 cggtaatcct tgcccatcat aacaccagtc tttccaagac cggcttgtgc ctccacagaa
    37561 ttctcggccg caatggaaat ctgaagagaa aaagccgaaa taggaacaag ttttaatggg
    37621 tttatgactt ggaccatatt attgctgctt tctagcagca ttgtttcgcc tgtctcttcg
    37681 gtgcccggcc atgtagcgag aagagggaag aaataatctt cggccaaaaa tgtatgcaca
    37741 acataggcaa aaggctcttc ttctccttcc cagatgagag ggatgacaag atgaaaaccc
    37801 gcttttgcat gaggagaagt aaggtacatt ctggtaaaga aaggtgtttt gggttttctt
    37861 tcggaaatct cccgaacaac ttcggaactg ttagaagtgg aaagattggt aagcgtatat
    37921 agaacgtcga agctcgaagt gagcaccgca gtatcggcat aattgtaaga gagaagagat
    37981 ggcctcaagc tttcatcgag gagctcgaga ttgtctgtcg aaggctctgc tatcgccctt
    38041 gagactagat ttaagaaagg cagcgaatgt ggcatctcct ttgcttccgt gatatgagcc
    38101 gtataccata tctcgatctg actaatcttg gattttccaa ttagttcgat gtagagtttt
    38161 ttatcgtgca ggatattttg ccattggcaa taaccgccta tcataagagt accgagcagt
    38221 atgataaaga aaacaacaag ccagaaatgc tcgataggac gcaattgcct aaggtgagaa
    38281 agaatattgt gcttcttagc attagactca gccactgttt agactccttc tcattcgcta
    38341 ttgccaaacc cttagtaaga gaaaggataa tctatactaa cacagaggac ttttatttcc
    38401 aagaatattg aatttttaca gtgttataat aactattttg tttgacaatt atgattattg
    38461 tgcttttttg atctaaagaa cattctggct ccaaccagcc aagctcgaga tccgagcttg
    38521 gcctaaagga gcactttcaa gtaatgatac aaatcaaagc ataatatgta aagaggcttt
    38581 tctagtccaa tttggccaga atcatccgag caagggtttg cgccgtaaat ccgagatatt
    38641 ccgctacctt atctcccggg cctgattcac caaagcgctc gatagaaaga atctgatctg
    38701 aaggagcaat gcgctcccag ccctgagcta caccagcttc ggccacaaac accttagatc
    38761 cgggcgaaag gatgatctct cgaaccatcg ggcaagactc ataaaaagca cctctgtccg
    38821 ggacagaaac aacgcggata gcggtttcat ggcgtttgct tttgacaatc tcggcggcag
    38881 agattgctag cgagacttcc gacccagatc ccacaagtgt cacttccgga gcgcgatcgc
    38941 tgtcttttac aacataagcc ccgagcctta tcttggaaga ccaatcgctt tcatgttttt
    39001 ctagaacagg cagattctgt cgcgaaagca caagcacggt aggtccatcc aatcgctcca
    39061 ttgcaatctt ccagctagcg cttgtttcgt ccgcatccgc ggggcgcagc acctttagat
    39121 taggaattgc cctcagtgcc gcaagcgctt cgattggctg atgcgttggt ccatcttcgc
    39181 ccacatatat cgaatcgtgg gtaagcacga agattacagg cagtctcatt agtgcggaga
    39241 gcctcaaggc cggacgcaga taatccgaaa aaaccaggaa tgtcgctaca aaggggcgca
    39301 agccgccata tagcgcaatt ccattggcta tagctgccat ggcatgctct cgcacgccaa
    39361 agtagatata tcgccccatt ggattggtct ttgaaaagac aaggggagta ccatccgtat
    39421 tttgcggcag ttggctcacg ttggggttcg tcagatcagc tgagccaccg ataaagttcg
    39481 gccaggcggt agaaatagcg gctagacact tgcccgaagc atttcgtgta gccaccttat
    39541 cgccaaccgc gaaagaaggc atatcgagct tatgcatctc tttaccgctg agccagattg
    39601 tgagttcttg ggccagggaa ggattggcct tttgccaggc ttcgaagcga agctgccagg
    39661 cgtcatattc cttttccagc tctccttgat gttccttgaa gtaagaatag gcctccggag
    39721 ctacccagaa gggcttgtcg agtggaatgc caagaccttc cttggttttg cgagcttctt
    39781 catcgcccag cggagaacca tggacaccgc tcgtaccctg ctttgtcgga gctcccttgc
    39841 caataatact atccaggatt atgagagaag gccggtcttt ttcggccttg gcttcggcgg
    39901 ttagagaagc gatatcctca aaattataca tggaaccccg aagcacttgc caaccatagg
    39961 cttcgaaacg cctgcctaca tcttccgaga aagttatatc agtaggacca tcgatgctga
    40021 tatggttcga atcgtagtag acaatcaggt ttcccaaccc aagatgaccg gccaaagagc
    40081 aggcttcgct ggctactccc tccatcatgc acccatcgcc ggcaagcgca aacacaaagt
    40141 gatcgagaat ctgatgaccc ggcgtattga aatgcgccat catctttcgc tctgcaatag
    40201 ccataccgac cgccgtcgca attccctgcc ccaagggccc cgttgttgtc tcgacacccg
    40261 gcgttcgccc atattccgga tggcccgggc atttcgatcc gacctgacgg aaagctttta
    40321 tatcatcgag agatagatca aagccagcca tgtggataag agaataaaga aacatcgagc
    40381 catgaccggc cgagagaaca aatctatccc tatttagcca ctttggatcc ttagggttat
    40441 acttgagctc gacaccataa aggtaagcac cgagctccgc agcgcccatc ggcatgccgg
    40501 ggtgacccga atttgccttt tggattgcgt cgatagtcag gctccgaaca gaaagagcaa
    40561 tagcctgcag tgcggcaata ttcatcgaaa tcctcctaaa aatactagat cttgacaaga
    40621 ttcattggat atccttgact ggattaacag gacaattcga acagaattga tgggatttta
    40681 tcccaagact ctcttatccc gttcatccgg cctcattgcg ttatcctgtc caagcatcct
    40741 atcatcgagt cgaatctttg taaaactact caatacccac gtttcgtcgg gctagctcaa
    40801 ttatctttcg cgcggtgtct tcgttcacgc gagcaatact gctaaggtat ccgggctctg
    40861 cctccgcaac cgcttcgagc gatccaagtg cattgaaaat ctttttagcg cgtctatcgc
    40921 caatgccagg aacctcttgc aacatatgga gggtagcatc gcttgacctc agtttacgcg
    40981 aaaggccggt ggcaaagcgg tgtgtttcat ctcgtactgc gacaagaatc ctcaaagaag
    41041 gtgagtcttt aggtagcact atcggatcgt tcttatcggg cagccatatt tcctcatttc
    41101 gttttgccag gcctatgata tcgatatcaa ggccgagatc gtccaggatt tccttggccg
    41161 ccgagacctg acccaagcct ccgtcgacga gtatgaggtc cggcaactcg gcttcttcgt
    41221 tgataagcct ggtgtatctg cgcgccacag cctctcgcat ggcggcaaaa tcgtctacct
    41281 tgccttcgac ggaacggatt ctaaaatacc tataattctt tttttctgct actcctccaa
    41341 taaaactcac tagcgaagcc acagtatgct ttccaccaag ctgagctatg tcgaatccct
    41401 cgatacgcat tggaacagat cctagcccaa gcctctcttt tagctcgaca agagcaacta
    41461 tgtctccatg tgcgcggcga cgctttgcca gttcttcttt tgcattttga accgccatat
    41521 tcatgactgc ccagtgtcgc tcgctgtccg gaatctttac ctcgacagga gtacctgcgc
    41581 gatccgaaag atatcgtgct attgttgctg tattgtccgc tcttcgtaca aagacctggg
    41641 gcggcggctg cctgccgatg tcataatatc gcgcaaggaa actgtccaga gcatcgagct
    41701 catcgacgca gtgttccata aaactatctc tattcttgag cttgccatcg cgcatctgaa
    41761 agatgacata gctagccatg tcgccgtctg cttcccaggc gatataatcg cgcgcgcctg
    41821 ggctattgaa atccaccaca ttcgaacgac ccgcaaacga ccgcaatgct tttatggcct
    41881 cgcgcaggcg cgcggcttcc tcgaaacgca accgctccgc agcgcgattc atgtcatgtt
    41941 ctaaatctct gaccagcgat tcagtctctc ctaaaagcaa tttccgtacc ttgtccacct
    42001 gcttcgtata ttcctcaagg ctaatcttat ccgcgcaagg agctaaacag cgcccgatat
    42061 ggtagtacat acaggggctt gtgtgttttt tgatattggc acagcgccgc agagggaaca
    42121 tgcgttttat taagtcgaga taggtgtcta tggtctccgc gcttggatat ggaccgaaat
    42181 actcgcttcc gtcatttatg atccgccttg tacgaaagac tcgcgggaac tcttcattcg
    42241 ttattcggat cgaaggatag gtcttgccat cctttaggct aacattgtaa cgcgggctgt
    42301 attcttttat aaagttgttt tcgagaagaa gcgcctcata ttcatgtgca gccagcaccc
    42361 attctatctc atgaatgtgc gacacaagat ggcgcgtctt tgcatccttt tttccggcaa
    42421 aatacgaaga tagtctattc ttgagtacct tagccttgcc gacatagatg acttcgtggt
    42481 tttcgtctcg cataagatag acaccagggc attccggtgc ctgcttgact agcgccttaa
    42541 gccttgcccg gcgttcggaa tccgtttcca ctattggtcc cttcaagggt tgtatagctt
    42601 ctcgaattgc gtctcgatcc cgggcttgtg gagatttctt atcatcgcat ttcttggcgt
    42661 atcttgcgtt catagtatat aattaaaata aggttcttcc atggaaaaga caaagcagca
    42721 atctgaagca tctcaaaccc caagagagcc ctttgccata atggcgagct ctgaattgcc
    42781 tatcatagac atagataaca atggttgctc agatgagtat atccgctcgc tggaagagaa
    42841 aacagcccat gctctcgagc gccttggagg catcggtgcg gctgcctcgg cttttcactt
    42901 ttgcccatcc tgtggcagcc ccaaactcta tagtgtaaga agtcggatgt ggaaatgccc
    42961 gacttgcggt tttgaatact tccacaatgt tgcgacggca gcgggcgtaa taatcgaggc
    43021 gaagaataaa atcatcatga taaggcgcca taaagatccc atgagaggca gattcgcgct
    43081 tccaggaggt ttcaccgagc ccggcgagcg agcggaggac gcagccctcc gcgaatgcta
    43141 cgaagagata ggctggtcgc cgacccacat caattttcta gccacatttc cgaatgtgta
    43201 tcaataccag gggattccct acgcgacatg cgatgtatac ttctattcgc gaggcaatat
    43261 cgtggaaatc gacgattttc acctggatcc tgaagagagc gacagtgtgc agtttgtggc
    43321 tgccaatgcc attccgtggg caaatatcgc cttcgaatcc acaattcgcg ctttgcgcta
    43381 ttttatcttg catgagagca taggtactcc gccgcgcttg cccgaatttg gcgccataga
    43441 atagagcatt tcaagtcagg accttctttc tatagagggc cagaaaaaga tagtcaatgc
    43501 tcaaccctgt gctcggaatc ttctagacat cctcccagaa tccagacctc tcggatttga
    43561 agagtgcatc gatcactttc atattggcaa tcgcatcctc tggaggagtc ggaaccgctc
    43621 gtccttcgat tatgctcttc gaaaaagcat cgaacatgag cctgtattgc ccggcaggtc
    43681 caagtcttac gatgcgcgaa cctatacttg tcgaaatgct catttcggca gggatatcat
    43741 cgtacatatt aaaaggtata aagatcgcta tggctccggc agatccgacg atatcgaccc
    43801 tttgcacagg gaacgactgc gtggccaccg tgaaaagcgc ttctgcatct ccaaagtcca
    43861 atatagccga ggagagcaca tctgtgccaa attccacatc ccgccgcacg agagaaatag
    43921 cacgcctcgg ctccttgcct attatgaacc tgctcgacga gcaagcatag cagccgatgt
    43981 catagatcgc tccacctccc gccgacagta tatttcggat gttgctcggg tccctattgt
    44041 tgtaagtgaa ttgcacatgc acatattgta gcttaccgat ttcgcctgcg cgcacgactt
    44101 cttcagcatg tacccattga ggatggaagc ggtacatgaa ggcttccatc acctttatac
    44161 ccttagattc cgcatagaga agggcatctt gggcttgggc agcattcatg gcaaaaggct
    44221 tttcgcacag tacatgcttg ccagcttcgg cagccttctt gatccattcg gcatgaagat
    44281 gattgggcag tggaatatag accgcatcta tttccgggtc ctcaagcaaa gcctcgtagg
    44341 atccatacga acgcgcaatt cctagctcgc ttgcagcttg accggctttc tccaggtctc
    44401 gcgatgctat accgaacatc tttgtcagtt ttgagcctga gagttgatgg tgcaccctta
    44461 gcctataatg attggaaacc gaaagaatcc cccatcgaac aaagtccata tcaacctcct
    44521 ataacataca ttgctatctc acggccttaa gttctttcct aaacctccag aagagcaaaa
    44581 tgcttgccac cgcaaacagc gtgtagctcg caaatagcca gcggtagcca aatatttcga
    44641 ccaccgttcc gcccaaggcg cttccgagaa aggccggcag acccattcct acacccatat
    44701 agagagccat gccggtagta cgatccgaag gcggcgtctt cagattgatg aatagaatcg
    44761 ccgctggctg aaaaagccca tagcaaatcg aatggagaag ctgacctaaa atcacgccga
    44821 taggcgttgg aaaaagcgca tatatgagaa gccttaccac aatggcaacg gaagatatcg
    44881 caattgccat catagggctt cttccctgaa tgagtttcca ggacagcacg atgaatggaa
    44941 tttcacatgc cgccgacaga gcccacatgg aacccacggc atgccaattc agttcctcca
    45001 tgagatacat cgaaaaaaaa gagctgatcg gagccatagc tagccgccca agcgctatga
    45061 cacccaaccc cgctagataa gttgcatcga gccacttgaa cgagaatttg atcttttcct
    45121 tccgtgcctg cccagcacca atctcaggca aagaaggtat actgagcaag aacaagagcg
    45181 caagaattcc caagggtatc gccatggtgc ccgtcgagct agaatcgaat cccggaatag
    45241 actgagctat cagcgctgta ataacgaaac cagccgagcc aattgcccta ataatgccat
    45301 aatttggcgc tttttctcct ttttcttcac tatactcaat agccctcagg agagaagtgt
    45361 ccagtacggg tatcggggtc tttattccta tggagagcag ggcaagactc aagagggtaa
    45421 ccaatggaag tcgaaaagga gctagcaatg caagaccaac gagaatcatt atcgccgaag
    45481 catatagaaa aggcttgagc tttccaaacc tgtcagcaat ctggccaatg aatatcggcc
    45541 ccgtaattcc cacgagttcg aaaaaaccaa gaaagaagcc aaccgtagca ggactatatc
    45601 cgagccttcg caccattagc tgcagataag gcgatgagat cccgtacatc gcaaagagca
    45661 taaagtaacc aagtccaaac cgccgcgata tattctgttg tttttcaagc tcgttcataa
    45721 ctacttgaaa gaatagggtt aagggagaga tgcgtcaaca tttattgcaa caataccaag
    45781 gaacatgtag aataatgatt gatggaaatg cctatcataa gtctgttgca tgctcattgc
    45841 ccttctaatc aaaagaccaa gaaatttctt atgatagctg gactcctcct tggtgatctt
    45901 tttgcagtca tggcaagttt ttgttcaggc tttttcctgg aaaacctaat cgatatagaa
    45961 gggatcaatt ttaaatcatt tgtcacttat tggccttatc ttcccgcatt tatagtcatg
    46021 ttatcgcttc tcaatctcta tcccggaatt actctttcca cgccagaaaa gctaaggcgt
    46081 tttactgttg catcgttcct tgctcatgca ggcattatcc tgtccttgtt tattggagcc
    46141 aaaagatttg accaatatca gacggcgttt ttattttctt gggtcttcag tattcccctt
    46201 ttcttttatt gcaggactgt atcggaatcg ttgcttgtaa ggtatagatt gtggggaatt
    46261 cctgccgtca tatttggagc ggggaaaacg ggcaagctcg tggtcgatcg acttctcgat
    46321 aatctcaaga ttggctatat tccagtgctg ttgctcgatg acaaccctct ccttgccggt
    46381 gaatatcgtg gtgttcccat tgttaccggt actaactatg gttcttttat tgcgtcttcc
    46441 ggagaggtgc agactgccat tatagcaatg ccgggggtgg aacataagcg tttgaccgaa
    46501 atcatagtcc gacaagccat gggattccgc cgctatttga ttatcccaga tgtcatcggt
    46561 atctcgagcc tgtggatggc ggtccaggat cttaatggaa tcttgggctt attcacagat
    46621 caggagcttc tcgagcctcg aaatattgct atcaagcgcc ttttggatat cgtactcagt
    46681 gttattggca tgatattgct ttctcctttt tttatcgtta ttgcgctttt gattaagctc
    46741 gattctccgg gtccgatatt ctatagccac cataggctag gcaaaaacgg caagccgatt
    46801 gaaatattga aattccgctc aatgtatacc aattccgctc aaatgcttga aaccttgctg
    46861 cgggacaatc cggaattgca gacagaatgg caagctaatt tcaagctaaa aaacgatcct
    46921 cgtataactc gggttggtgc ttttttgcga aggacaagtc tggatgagtt gccgcaacta
    46981 tggaacatcc taaagggcga tctcagtctg gttggtccca gacccatcat tgataacgaa
    47041 gtggaaaaat atggggacaa ctattggcta ctctccaagg
//
LOCUS       DAOOWY010000005        42826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_943_length_42826_cov_10.639242, whole genome shotgun sequence.
ACCESSION   DAOOWY010000005 DAOOWY010000000
VERSION     DAOOWY010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 42826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42826
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_943_length_42826_cov_10.639242"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1720)
                     /locus_tag="PK105_00880"
     CDS             complement(242..1720)
                     /locus_tag="PK105_00880"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HOU60286.1"
                     /translation="MIEHTLLYNFRISEDAASALDKIPRCIEEANPISFQHRAELRYS
                     IRSLWRLLTMGRAQRASDYLNNPTLFSAYLRYFMPWNLVRLVSLLTDLPVELKPNATI
                     TDMGSGPLTLLLSLYCSKPELRKLPLTIICVDRAPRMMETGKTILEILAAEHSGELPP
                     WKIELRHLRFGEPIREKADLFCAINVFNEFFWKSKETLFENADALYRSIMQYCKNDGS
                     LLILEPGEPRCGGFLSALRAVAIRDNCEILAPCPHTSACPMPGIFKSGQEYLIKENPK
                     NIIKKGIPRFDSIESKANVARQKSEYIIKQLPDESSNHRNRFLTPVRMPAPRVKYPWC
                     HFSMPTSYAPKWLYRLSQEAGLAKDKLSFSYLYVKRCPSSILPSDQNKKQKSRYPGNL
                     PLPTGANEGYDELACRIVSEPIVLSHSRVGKYACSHLGYTLISTGQSEALPESGALLR
                     IATKIPASTSSSKKRKKEMEYDQKTGAILISYESPKKFSGRK"
     gene            complement(1751..2776)
                     /gene="tdh"
                     /locus_tag="PK105_00885"
     CDS             complement(1751..2776)
                     /gene="tdh"
                     /locus_tag="PK105_00885"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOU60287.1"
                     /translation="MKALVKRNAEPGLWMEEVPIPHIGDNDLLIKVKKTAICGTDVHI
                     WKWNTWAQRTIHIGQIVGHEFVGEIVDKGRSVQDYKIGERVSAEGHIWCGTCRACRAG
                     KHHLCPNTIGIGVNRDGCFAEYVAVPAQNAWHVHEAIQDDIAAIFDPFGNATHTALSF
                     DMTGEDVLITGAGPIGCMAAAIARHVGARNVVVTDVNPWRLELAKKLGATRVVNVDKE
                     DLKEVQKSLGMVGGFDIGMEMSGNPKALEQMIENMYNGGRMALLGLLPDGAGIEWSRV
                     IFKGLFIKGVYGREIFETWYKMQTMLLSGLDISPVITHRFRFDDFEKGFEAMLSGESG
                     KVILNLE"
     gene            complement(2990..3889)
                     /locus_tag="PK105_00890"
     CDS             complement(2990..3889)
                     /locus_tag="PK105_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU60288.1"
                     /translation="MERFALLAAFGTAVCWAFSAIFFETASRKVGALAVNFWKVVFAF
                     LFLGISGMVERNVPFPYDAPLRTWILLGLSGIVGFVISDFFLFNAYILIGSRITVIFQ
                     SLTPIFTALFAFIFISERMQVHRLIGMAVTVAGILIVVTTRSRQNKKNNKEKLSAKGL
                     LYGFLSSIFQAGGMVLSKAGLGNYSPISGTQIRAFVAIFGFAINALLIGQGRFVFVEM
                     PRIKEALRSTLKGSIFGPFLGVALSLFSMQHTQVGATSTLMALTPVVIIVPSVLILKQ
                     KVHYLEVLGAAVAVVGAALFFLL"
     gene            4036..4857
                     /locus_tag="PK105_00895"
     CDS             4036..4857
                     /locus_tag="PK105_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOU60289.1"
                     /translation="MAVIAISRELASYGEETAQELSKINGYKIVDKEHIEAALSAMGI
                     DVEKQARYDEKNPGFWASLSQQRDEYLHFLTQTVYDTALENNCIIIGRGAHIILRGIQ
                     NLISIRIGASKTVRIERVSKAQNIDSRHALQIIESSDHDRAGFHKYFFSVDWYNPSEY
                     DMTLTTDRFDPIHAASAIDAFRVSFITEDREKEAQAKLRDLLLGAKIVTEITYNRKIP
                     IHFLEAAVERGVVVLHGVANSRATVESAVAAANLVPGVKQIESAIQLVQEFTVIP"
     gene            4909..5652
                     /gene="rlmB"
                     /locus_tag="PK105_00900"
     CDS             4909..5652
                     /gene="rlmB"
                     /locus_tag="PK105_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOU60290.1"
                     /translation="MEYLTSFHAILETLKAGNSRCFLYITENSQRRGPRIREILDEAL
                     SQNISIQYVPESRLDEMSKDQRGAILALEEVFKKKPSLKALCDRVSEPRPSLILVLDH
                     IEDPQNLGAILRSADAFGVDAVIMPQRRASPISNAAARASAGAIAWVPILRLSNLRSA
                     VDTLKKAGYWVYAADMDGKPLTSAGIAPKSVIILGNEGSGVSQILRKVADDIVSIPMQ
                     GHVESLNVSVSAAILMYEFWRSNSGASSI"
     gene            complement(5765..7345)
                     /gene="pyrB"
                     /locus_tag="PK105_00905"
     CDS             complement(5765..7345)
                     /gene="pyrB"
                     /locus_tag="PK105_00905"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOU60291.1"
                     /translation="MARNGPFKGRSISVVNDLSLDEQLYLYRKARELKETARGGHDAE
                     KFRINDLDYQVYLIFMENSTRTRESFRNAGKFLGARVNVFDAATSSFNKNESITDAIK
                     MLFGYSNESCFILRTPLEGVCTWLDQAFEEYAHITEKLKPSFINAGDGKHEHPTQEFL
                     DEFSFLEQLNWKEEHIHIAMVGDLYHGRTVHSKADGLRIFKEVEVDLVAPSLLSMPSY
                     YVDKMRASGFQVNIFESIDEYLAQDNIAPIWYFTRLQLERMGEAVLERAPYLRQSVTF
                     KKDYLGRLPENCRFYHPLPRDRNYPTIPFFLDDLPLNGWDGQSINGYWTRITEIAMLS
                     GRIGGDFEGEHAKPREFIDDFVHEASIQETSKPEYKVGIKPVEEGIVIDHIARGASIG
                     EIWDTIDAVRKILKLDVRSSHGVYHSNRGEGIYKGIISLPDIISFGEKDLKKLAAIAP
                     GCTLNLIRHAHAAKKYRLSMPPRIYNFDEISCKNENCISSPAQHEGVPTEFIRKGETT
                     FVCKYCEREHKFRDIWDI"
     gene            complement(7335..8507)
                     /locus_tag="PK105_00910"
     CDS             complement(7335..8507)
                     /locus_tag="PK105_00910"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOU60292.1"
                     /translation="MIADTLTLRVEKLSSNGEGIAFSEGKAIFLPYTIPGETVLAEII
                     EEHSGFARARIIDLKESSPYRVEPPCHHFGDCGGCSLQHVGYEHQALLKKQAALDTFL
                     RIGGFDPGELQIIIGEGYHCRNRAQIHACGDGGLGFAKTGSHESVRTSHCPALVPIIE
                     RWLVSENRKAHPYRALSALIGDRPRFVVFGQENRIYLEGRDNFAKAKVAGKELRFPLA
                     GFFQSNIFMLERLIAENVISLYGKRALDLYSGAGMFSLFLADRFEAIDCVESEATSIE
                     AARMNLESAATKIEFSCTTVENWIEAARSSLSFDCILADPPRTGMTPKVRTWLSFVEA
                     PMLLYISCDLASLARDLRDLFQKGWQLENIILYDFYPQTGRLEAAARLIKEAKHGP"
     gene            8565..9986
                     /locus_tag="PK105_00915"
     CDS             8565..9986
                     /locus_tag="PK105_00915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU60293.1"
                     /translation="MSINIRSLGAAEEVTGSKHLLEVDSTRILIDCGAFQGKRAKADA
                     KNRALLGDDIEASSIDALVLTHAHYDHCGLVPYLVKRGFTGNIYATSATRDLANLIMT
                     DSAHIQARDADYLSRQAEKRHEQFDWKPLYDNFDVIRAMDQFVTVSYHRPISIADGVQ
                     IEFFDAGHILGSAFARITAKDKAGNVAILGFSGDLGRKGKPIIKDPECLNGIDFLILD
                     STYGDRLHESTADSLLHLEDVVNRTAKKRGKIIIPAFAVERTQELIFSLHLLHDQGRI
                     PDIPIWVDSPMAFNATSIFVIHPECYDKETYDLFTAHAKNPFGFAALNFSQSVEDSKA
                     LNQAKGPLIIISADGMCEFGRIQHHLMHGLGDSANTVLIVGYMAEGTLGRRLRDGAKE
                     VRIQQDWYQVRANIEEIDAFSAHADWKETIEWLECIDKKRLQRTFLVHGEQEALMAMQ
                     GHLIDADLKDIEIVKMGVFYKIA"
     gene            complement(9994..10425)
                     /gene="rpiB"
                     /locus_tag="PK105_00920"
     CDS             complement(9994..10425)
                     /gene="rpiB"
                     /locus_tag="PK105_00920"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOU60294.1"
                     /translation="MKILIANDHGAVALKFNILRWLETQGHVVVNLGVDTEDRVDYPD
                     QAALAVTEYFKGSYDLGIVCCGTGIGISIAANRFKGIRCALIHDSYTARMAKEHNNAN
                     FLAFGGRVDYKEPVEELLSVFLHAEFRKGRHAERVAKLDTI"
     gene            complement(10494..11354)
                     /gene="mtnP"
                     /locus_tag="PK105_00925"
     CDS             complement(10494..11354)
                     /gene="mtnP"
                     /locus_tag="PK105_00925"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOU60295.1"
                     /translation="MEKAKVGIIGGSGLYEIEGAEVVEEIKVDTPWGSPSDLITIAKI
                     GGVRTAFLPRHGKGHFLLPTEVNSRANIAALKMLGVREIVAFSAVGSLKEDIHPRDFV
                     LPSQIIDRTKDRPGSSFFGEGIVAHLSFADPFCTRLCSIIEPLILARGLRLHTDETLI
                     CMEGPAFSTRAESQLYRSWGAGIINMSVLPEAKLAREAGICYAMICMATDYDCWKMDH
                     ADVTVDMVVQNLNANSAHAKALILDIVPALSKDTECSCHTAMKGGIMTAPSKRNPETV
                     IKLKTIFPEL"
     gene            complement(11404..13134)
                     /locus_tag="PK105_00930"
     CDS             complement(11404..13134)
                     /locus_tag="PK105_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOU60296.1"
                     /translation="MIGYTCKYTPIELLYALGETTELLNHEVPDFEYAEKWTHPNLCC
                     HAKAFLQHSSELDELVLVNCCDSIRRAYDVLVAQQVHRFLFLFDLPHIDKGCAQEKIK
                     KELFRLTRLYTEHHPVSFDRRRFLEACRASSEVAFKASFESASGTHSKLDPATPFIAI
                     IGARVSQELLSTIKEEVPYLVIDATCGGNRYLSPLAPGAERMSFEDLMDWYAKELLHF
                     IPCMRMANIEPRKALWENENLCGIIYNTVKFCDYYSFDYARVKREVRLPILKIESDFT
                     PQSEGQLSTRLEAFQENLHLSLEKPLKIKARQASQPAFFGGIDSGSTTTKMVIIDRNS
                     KILASSIVSTGAKTQVSARKVLQETCRQLGVEPCDLASVFSTGYGRNNIAFATDSKTE
                     ITCHAKGAFFLDPTIRTIVDIGGQDSKIIRIDNEGRVVNFVMNDKCAAGTGRFLEMMA
                     RTLELDIDIMVRTGLAWKNDLTISSMCTVFAESEVVSLIADNCSIADIVHGLNKSIAS
                     RITSMISRTGCQAPYMMTGGVARNTGVVRELEVKLGERLIVSEKPELCGALGAALSAL
                     ELSALGSKEI"
     gene            complement(13131..14402)
                     /locus_tag="PK105_00935"
     CDS             complement(13131..14402)
                     /locus_tag="PK105_00935"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOU60297.1"
                     /translation="MNSIVHRFGRVVSNQVLENPQRARSLLCTVYALSSWKDAYFRGR
                     GLRGIYARMNSVISKNMSRSFRDSSHSAMVNIFFPCELLYAMEIVPVFPEALSVYIAN
                     SGCGEIFAESAEAIGISESLCSYHKIMIGTAWTGVLPKPLLIANTTLACDANQLSFRL
                     LADEYHTPHIIIDVPHTVDKESVHYVSSQLKNITSLLEDLSHCPMAESKLQDTIDRAN
                     RTIKAYRKYLDLRAEISLPISMTGELFSLAALHAFLGMPESEQYAIALEELAHKASLD
                     RRKKRILWLHTLPNWQHSIRVLLENNEHFEILASDMTYDTLIDMDPEKPYESMAKRLV
                     YNINNGGAHRRIDAAISYAEKLHADGVIVFGQWGCKQTMGMSQLAKISLEAHGFPTLV
                     LDGDGCDPRNVADGQMLTRISAFLEQLEARR"
     assembly_gap    14530..14539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14676..15653)
                     /locus_tag="PK105_00940"
     CDS             complement(14676..15653)
                     /locus_tag="PK105_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU60298.1"
                     /translation="MIVGAIEGGGTKFVCAVLQFDHPNIPEKTPRILRIERIPTTDPE
                     PTLDAVVRFFKDSEIECVGLGMFGPIECRIESPLWGYLLKTPKPGWSDIDIAQRLQRK
                     LGVPIKLETDVAAAGLGEWKWGAGLGTHCCVYITIGTGIGAGIIIDGIPMRASFHPEI
                     GHMRVPRARTADSGPDEFKGLCIHHGDCLEGLASGPAIAARWGEAPENMSIEHPAWKL
                     EAEYLAYALTNIALTVAPDRIIVGGGIGLREGLLDLISERMRELLGGYIQALNSTAAI
                     RLFLARAELGSKAGVLGAAELARQTINSVSSVSNRLYTFGKSRSSKNQG"
     gene            complement(15664..16215)
                     /locus_tag="PK105_00945"
     CDS             complement(15664..16215)
                     /locus_tag="PK105_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60299.1"
                     /translation="MFSSLDFKEYEQVAGNSGAFKGVEIDLMLEILKDWQQAPGKNYT
                     LLELRDGKTLVAFAIIHKAAGRNFTFDIRFVVLDSDYRSTNAIQHLFEMIDQELLKKM
                     AFAVIRFETSTRKRDSLGENTLENSGYSQIGHIVDFYGANEDFFFYIKAIFRNPPNFI
                     KISKPFEEDLCSECLPEEIDNQK"
     gene            complement(16483..17739)
                     /gene="pepT"
                     /locus_tag="PK105_00950"
     CDS             complement(16483..17739)
                     /gene="pepT"
                     /locus_tag="PK105_00950"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOU60300.1"
                     /translation="MIAILDYLNRSFTEEMLRRFLSYVRIDTQSNRHAEKTPTTEGQW
                     DLARQLEQELRELGISELELDRHCYLIAHLPASPGKEHVPCIGLMAHMDTASDVSGSG
                     VKPRVIKNYDGKGIWLSEQYLLDPGEFPDLYEHVGDTIVVTDGSTLLGADDKAGIAEI
                     MTAVAYLLQHPELKHGPIDIYFTPDEETGKGMDIFPLGKARAKACYTLDGGAAPEIEA
                     ECFNAYAVKADFFGKVIHIGAARGRLANAVAMAASFIDMLPRSESPEATDGWFGYYCP
                     IEVSGGLEHAWTEVYLRDFSASRMQERIAALKAFAAAVEAQFPQGHIELNVTQQYLNM
                     KEKLDKHPEVLERLKNAIKVLNIEPIMKPIRGGTDGSRLTEMGIPTPNLFTGGYNYHS
                     RFEWASLSEMALSVDTMLNLVQGWAD"
     gene            17898..18851
                     /locus_tag="PK105_00955"
     CDS             17898..18851
                     /locus_tag="PK105_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOU60301.1"
                     /translation="MTIPKYPEFAPLTLEARDILYPALNLLPDGISEFTFSGLYLFRN
                     TYGYEISQISDEKFIISGRYKGRTFFYIPCCFPSMEQFDELMLNFDYMRHLSECQAAK
                     QRIELERRGYLVREDRDNFDYLYNREDLATLNGRVYHKKRNLVNGFVSNYACEQRPLT
                     KDRAADALQVLEEWRAAKGADGDYAAAREAIECFNILGLRGAVYYIHHNPVGWCLGEP
                     LAKGKMFAIHFEKAIDSYKGIYQFINQAFAQSLPKHYLLINREQDLGDLGLRQAKMTY
                     RPVGFVKKYTVERPGLANIVPAPIEPPAECGPGTVHACDED"
     gene            complement(18864..19376)
                     /locus_tag="PK105_00960"
     CDS             complement(18864..19376)
                     /locus_tag="PK105_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU60302.1"
                     /translation="MEILKISLAFFKIGIVAFGGGWSVVGLIKQEIVPSWMSVDAFSS
                     LIAIAQSTPGPIALNAATLVGWQKAGFFGALAATFSVIAFPLLAMSLTLAFSRYIPIK
                     KDLADESLRSGSMAMIIMTLWTLLPQNTIPMPWIFALASFGITAFTQINGVWVILGSG
                     AINMLLGLLP"
     gene            complement(19364..19948)
                     /locus_tag="PK105_00965"
     CDS             complement(19364..19948)
                     /locus_tag="PK105_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU60303.1"
                     /translation="MKIPSRKNGAKKTTKKTEPTPLRIFLTLAQINTLTLGGGYVIVP
                     VIGNSFKKQGWIDEEEFYRIFSRAQVFPGPIALSTSFLCSYKIAGVKGAIAAVLGVVL
                     PPFIALILVGGFISLYGDTELFKRFLKGAGAVVPGLVGSMLWKTAKNRKWTTRALIEV
                     GLLTILLVLFPSAAFFILIGGIVVIYLGRLIWKS"
     gene            complement(19962..23684)
                     /locus_tag="PK105_00970"
     CDS             complement(19962..23684)
                     /locus_tag="PK105_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU60304.1"
                     /translation="MISHHDSFDIEALKLDDFQKKAAYTEDTAIVTAGAGTGKTRALV
                     GRFLYLVLQKNVDPERILALTFTRKAAAEMFQRVQQALSPFANDRPNLARSLMQTHIQ
                     TLDSFCHEIVAQAAVLYGYTPDFQIDESKCARIAEKTAYRFVLDNQKRDGIESLVFAF
                     GFQAVVQDLFASFGRANVEPHWKGKQICRASARIGEQEFKKAAEEIAEKIHDIALNIL
                     EIANQKPGNYAIHGFREGTNQAIRAARTVSCLSYDLKGLKDFSDYMEKPENAINLQSV
                     GRNETESAIKELAKELKNKALRDRISQLVDFEKFLPSYYAIMDRLDEYADMLCEEKCK
                     ANIMNYKDLGALAVDILSNHEDRRIFWASRFQYILIDEFQDNNELQKQLLFLLSKEGK
                     EIRRGKLFFVGDEKQSIYLFRGADVSVFKGLANELMAAPLSLNLNYRSTNQLIEFFNS
                     VFRIVMLPANPENPQPFEALYQDMETISTENANIEESKGFSSRIEYHCITNKIPAEEE
                     ACGIRSAKESMAFRLALWIRDAVESSNPLLIRKEGETQDLAITEKKQHKLSLRKAEYS
                     DIAVLMRTTRRQYELEKYLRMLAIPFVSDTAASLFSEEPINDLYYILRLLIDAKDLFA
                     TAAVLRSPLCRISNDGFVSILTEKKSLADLEADMPIKLNPNDRDSIERMVLFYQELRQ
                     KSDHMPLMELVAYIWKEGRLEASILCDEQRLPYLDHWNAIRTLAANTEAEGGHISAFL
                     ISIRDYMQNMHLFDTNSVPKAEAHGVHLLTIHKSKGLEFPIVIIPWTEASTNKNGGKE
                     LWGILESAQEKGRTRFYTVDIGFHDRIQSGSNILQTQAKAQRTEKEQAETKRLLYVAC
                     TRAIDHLIFFSAAPENKPYDRDSFHALLFQSTAHLIPRDYSEKTQLPAHLSGLQLFFE
                     PLVSDKEIQGARSNVLRARKLSQKKLLSEKEKQESEQKSKEALCKTAPKTEKSFIEPA
                     KMTNLPSDSVAGSHSRRITASSINAIIAQQSLAADYARELSGHFVSKRIFSPAESRAQ
                     SEAQALDETSLEAQNTQESLDFSAYGSLVHELFVHILAEKTMENFRPSASLSRELLGS
                     TSPEFGDTSIAKKTDPRYLSSIIENAAAELAPLFEAPTFSTLVLEKKYSCEFPFLLGL
                     GRWIIEGRMDFVAQSDEEVIILDLKTDRRLLPHKYALQLGLYCIAAQQLFQKKKVHAG
                     LIYLHFGQIAWLNANPDIEILLKICDSICFDTHVNS"
     gene            complement(23684..26554)
                     /locus_tag="PK105_00975"
     CDS             complement(23684..26554)
                     /locus_tag="PK105_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOU60305.1"
                     /translation="MPKSVDSTKIAEGYKSLLGTPLGRDISQHLQDENIVFVFPSQDT
                     ADSWARAVVYSEISRAVALNRFLGFDNFLLYVAKANEQDDLSVLKPIHRWIWAMQILS
                     KEQKGERKPGKRLSTLLPSMEISTKNLTRLVRLAPYLFEIEMLTKDSAVLDASPCRFI
                     KEEFEEMRLLAEDYIDFLDRARLRDSHRRPLVLPPFMKVRAYGLRDDFIRFGLIDSEE
                     LGDGNINFRGILGEETGHEKSLSAKERIAFPDIFTQERGEKAPMLYHQFNSSLVEIEW
                     ILEEISNEIEQGIEPEDIAISVCGLTAKKEAWIRQIAAEYGVPVSIRRGKPLSMSPFG
                     RVLMAIQNAAREGLTLESLDAFADLANITGRDPEAWKALRDTALSLRLPSPSPSAAYI
                     HSLWKGAFGTGQCPESTAQIYQRLWESILSIARADSFSRLYDQIIGFLEHWIDTSGFG
                     ANIQTDRSMRLTLDELQSWIDHENIFTDLSIPPFELFLTVLESKSYIPLVEENAVYVY
                     DFDTTAGIAVLSHYVAGASQEGLASSLKIKSALPPELAGILQQEKTDKAHEVINMHRV
                     SPTQFSFAAKGFEDYETAYPLFPLAIMIDPNAERGLRAILISKEQKSATESYSHPEPI
                     EPTVARSFLNATKSFDSEKKYAIFSPSSLKELSQCGFRWFASKIDLVDVYSRDDSAIA
                     IGNLYHSAYRRAIQALPQNFKNAETDEEFRKTINSALSIEAKRILLQYGSGLQPILSA
                     LFPRAIHRLNELWNFERTMFSAYEREGFEMRLQHEFSEEEAILEGRIDCLFSRQDESL
                     GNIKCYVIVDYKKNRIPSISKMRVGVMPSDYEELASYDENQEKEDGYDSSAFFLEEIQ
                     VPSYTLLVETSWGRVEGAFYWSIEKAEAVGYIRPPTVPKMRSAYTYSKETDFQRSALR
                     DMLRVASSKVKAGNFLDLALDRAVCEDCEFKPLCRFWYFLEL"
     gene            complement(26601..30113)
                     /locus_tag="PK105_00980"
     CDS             complement(26601..30113)
                     /locus_tag="PK105_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU60306.1"
                     /translation="MALQILREFRSQNIPPKHLWMPSGAISLPDAVSARFVAQKYKLS
                     AGELRALSLIGEAFRIIIDLYRKQYSKLLEEIALKAFASTEDNKALWEVLQELITEFP
                     PAPIYDGLAEPKDWLKSLSPVGDDSSKPNLELAIEQLILVRLFNENPAFWPYRSLFDD
                     GVSPAGATLPDSISAKTPYLQVFARLEDALKTLPGLSYGGGKTLDLINFLREPSRHAP
                     ASLKDQLEWIIKNWGTLLGDFKLSLLAGIDMINEETRPHFPPGPGLAVPYQYRSSFHE
                     YEKFSPDKNWMPSLVLIAKNALVWLHQLSRTYSREISRLDQIPEEELIIMAERGINGL
                     WLIGIWQRSPASEKIKKLCGNSEAAASAYSLFDYEISPELGGWEALDRLREQCGQYGI
                     RLAADMVPNHTGIDSLWIRTRPELFMSLPYCPFPSYSFNGPDLSGDPSIGIWLEDHYY
                     NRGDAAVVFKRLDRHTGEVRYIYHGNDGTGMPWNDTAQIDFLNPASREAVKERILSVA
                     AHFNIIRFDAAMVLAKQHIRRLWYPAPGSGGAIPSRSDHAMSEEAFDKAMPNEFWREV
                     VDLCAEKASDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLKDEKNSLYRLTIKNT
                     QEFDRDILKRFVNFMSNPDEETAVAQFGKGDKYFGVATMLATMPGLPMIAHGQIEGFT
                     EKYGMEYKRSYWDETPDRDLIARHEREIFPLLRMRRLFSEVENFYLFDYMQDDGTIDE
                     NVFAYCNGQGERRVLVFYNNHWERTLGRIHTSCAFARKTADGKKQLKTTSLANALKID
                     SSPKNYVIMHEIRSGLWYIFRSEDIASRGFKLALEGYQNKVFIDIMNVHDTEGRYTKL
                     FEIVDSRGIADLDDALLEADQPELYRSLHNAINSLSSIETIQNLSQEEAIQRATIFSE
                     IFFSRLCEIAGSDDTLAASRSDSVRSASSWLKTVLKLLYGTTESDAGIAAATLCNNSS
                     ASNSEYQQYRLILLIYSFMRSLVKAFAADELNEEVSRVVKEYRIAKKLTESAVGLSRR
                     NDSHDTKYHVSICAEIAIAWALRQDKLFFDTRRTIDSTLTPDKRAQEICAWAFSDPLM
                     REALNINQYRGTEYFNKERFEAFASLLPAFAWIDSIQEETKEREWKEDPSWKEVAEIL
                     KENAIVAGYRTGIMLELMASVAQT"
     gene            30243..30875
                     /locus_tag="PK105_00985"
     CDS             30243..30875
                     /locus_tag="PK105_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU60307.1"
                     /translation="MSSQVAHEKRKQEILEKALDVFVEEGYADTTFQKIAERCGITRT
                     ILYLYFDNKKQIFNESIKRFLSSLEAEIALIARDQYLTNGEKITKISEILVEACERES
                     KMLSVVMDYLLRLKASGGNPDEKVRRRTVRMRHILADIVIEGIKCGEFKESSSVKGTV
                     EMFFALVEAAVFRLVVLGRKEAAGLQEALTPFVDNMLNPNFERNIENKED"
     gene            complement(30872..31264)
                     /locus_tag="PK105_00990"
     CDS             complement(30872..31264)
                     /locus_tag="PK105_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU60308.1"
                     /translation="MAHNMRPSLAFAVAYVESRYNPRARSTNRTGTLNSGLFQLNSSV
                     YGKISEKSIYDPYQNARLGIGHLQEYINLNGDEFSALAAYNAGITRISEKGIPGVTFR
                     YISEVSIMEHKIMNFFIASIALSESMKL"
     gene            31808..32428
                     /locus_tag="PK105_00995"
     CDS             31808..32428
                     /locus_tag="PK105_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU60309.1"
                     /translation="MARVKTKEKRTLILETAKRLFATKGESVSMNGIAKEIGISVGSI
                     YTYFPSKQSLIETIIEEGWDEYRVWVEDRMVSIMESGKQDDGIGQSLCALSFLVDEAL
                     PKLFSDVELIMILLAGASSNARLEKKLQYLSSMISKLVEDSSVSLEVPLDAKFYRIGI
                     MVFLLGALETIRITSKTDIDINAADIRAFLRQLMENALGQPLPDRD"
     gene            complement(32349..33392)
                     /locus_tag="PK105_01000"
     CDS             complement(32349..33392)
                     /locus_tag="PK105_01000"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU60310.1"
                     /translation="MRILVTGGFGNVGTSAVGALLAKGHEVLIFEQPSARSKKQKRTR
                     ELLKNAHHRLTIFYGDITDKKSLQDAICSAGVGGIDAIIHLAAIIPPLSNQKPLLAEE
                     INVGGTRNIIEICKSLAVPPRIVFASSVSIYGDRLANPWISATDPLDPNDNYSITKNK
                     SENLLRESGLPWVILRLSYVVSSDWLPFDPMLFDVPPSTHFEVVHTEDAGRAFAMACS
                     LSNIEGRTFNIGGGPACRTTYRAYLDRLMRAFGLGNSSFLCDEIFARGDFHCGWFTDS
                     EEAEMLLHFRSKSLEDYYSEVEWRTRFIRPLGSIAAMAVKPWIRSLSPYRAKADRAHS
                     PLIVSGKPEYRQH"
     gene            33527..35332
                     /locus_tag="PK105_01005"
     CDS             33527..35332
                     /locus_tag="PK105_01005"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOU60311.1"
                     /translation="MITIDELRRKYIEFFKARGHAEISGKSLIPENDPTVLFTTAGMH
                     PLVPYLLGEPHPAGKRLTDYQKCIRTGDIDMVGDSSHLTFFEMLGNWSLGDYFKNEAI
                     HWSYEFLTSPEYLGIDPAKISVTVFAGDESVPRDDRSAEIWKSVGIPEERIFFLPRED
                     NWWGPAGEIGPCGPDTEIFIDTDAPVCGPDCRPGCHCGKYFEIWNNVFMQYNKKADGS
                     YELLAHPCVDTGMGIERTVAMLQGKKSVYEIEAFTPILKVIESITGKTYGASVGDPEI
                     DRGFRIIADHIRAATFILGDPKAVLPSNIGAGYVLRRLIRRAVRHGRKLGIDGLFLSK
                     PAQAVIDIYKGPYPELLENASRIYEELEREEKKFLETLQKGEHEFEKILPNLLKNPDR
                     IMPGRLAFKLYDTYGFPIELTEELAAEHELRVDRLGFEEAFKKHQELSRVGSEQIFKG
                     GLADNSEIVTRYHSATHLLHKALRLVLGNHVAQKGSNITAERLRFDFSHPAPMTPEQI
                     AAVERIVNEQIARDLPVTMEMMPPEEAKSVGAIALFGEKYEPIVKVYTMGDFSKEVCG
                     GPHVSHTGEIGKIKIIKEQSSSAGVRRIYAVLEQT"
     gene            complement(35335..35817)
                     /locus_tag="PK105_01010"
     CDS             complement(35335..35817)
                     /locus_tag="PK105_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60312.1"
                     /translation="MEIELRILHLCRNIDYVHIDEPLIIAAKALSDILCCQSELLLEF
                     EEEGLSTMTEDGPKVFDTLEPSAVYRLAESKYIEQTSLNNLDLGRTSPFSLGIGEYIF
                     CQQDMDCTEGEWRLKSLLEEAIREVWWQRVECEGSWFLRLVPEDDRVAVQALRRMKAK
                     "
     gene            36044..38002
                     /gene="dxs"
                     /locus_tag="PK105_01015"
     CDS             36044..38002
                     /gene="dxs"
                     /locus_tag="PK105_01015"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOU60313.1"
                     /translation="MSLLNQISGPADLQGLSMADLRCLSREIRLSIIETVQRNGGHLA
                     SNLGVVELTIALHRVFQSPYDAIVWDVGHQCYSHKILTGRAARFVTLRRNGGLSGFPK
                     RKESKHDIFDTGHSSTAISSALGLLQARLSMGHAGRVIAVVGDGALTAGLSMEGLSNA
                     GQLSLPLIIVLNDNRMSISNSVGSLSRYLSKLSASVQYQSLRSRVDTMVLKIPRIGGA
                     IFSFINRTKRAIKAIFFKENLFVDYGFEYVGPIDGHNLVSLINAFEHIRNLNRPVVVH
                     VVTKKGKGLEKAEEDPESFHGLVPAYPDIPGPGTTSHGESFTSCFANALIACAEKEPR
                     MVAITAAMSSGTGVSKLAEHYPHRVYDVGIAEQHAVAFAAGLAQGGLRPVVAVYSTFM
                     QRAVDQVFQEVALAKLPVLFALDRAGAVGEDGETHQGIYDIVLFKAMPNLVFFAPADG
                     EELLAFLDLALTLDMPCMMRYPKAFSPCPDLPIAELEIGRGVFMREHEDARVLILAVG
                     PLSYIATDVSDELAIKGIFADVYNVRFISPIDEEYLVKICSRYEGVLTIEDGVLTGGL
                     GESVAGILMRRGIKIKISSLGFGSVPPPQATREELLSGASLDAKGIQKALHELLDFLD
                     ISKQQIAAGIPTDLSGTLSTSSEIQHAH"
     gene            37992..38876
                     /gene="folP"
                     /locus_tag="PK105_01020"
     CDS             37992..38876
                     /gene="folP"
                     /locus_tag="PK105_01020"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOU60314.1"
                     /translation="MRISANRTQLKHDVNRLRLPNGRSLELGKAPEHLACIMGIVNIT
                     QDSFFEGSRRPDSIAAIETALDMVKQGASIIDFGAESTRPGSTEIDSEEELSRILPVL
                     NGFRALSDAVVSVDTRHPEVAEAALAAGADIINDIEALQRPGMIETVSKAKAAVILMH
                     MQGTPQSMQKDPYYKDCLQEVRSFLEDRSHRAIEAGISPDRIILDPGIGFGKLKEHNI
                     SLLKGIEHIASLGYPVLIGLSRKKLIGDLTGRDVASRLAGSLGAALAAWLNGAQILRV
                     HDVAETVDALRAFHEVIQ"
     gene            38873..39676
                     /gene="cdaA"
                     /locus_tag="PK105_01025"
     CDS             38873..39676
                     /gene="cdaA"
                     /locus_tag="PK105_01025"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOU60315.1"
                     /translation="MNAPTLIEFFTIYVRPVLDVGILAFLIYKTYQLLVMTDAVYLIR
                     GLLILLAIYGISFFLKLSTVTWIMNILAPGLVIGLAIILQPELRRIFIQIGRQSIFGK
                     KSMARSSWIEAAVSASVYLAEKRRGVIIVMARKVGLASYIEKGIPLDAIISTSLLISV
                     FEHDNPLHDGAAIISQGRLAAAGCFLPLSDQQEIRKTFGSRHRAALGIAEESDAVVLV
                     VSEETGALSLAYDSKLYYDLSSEEILERLHILFSEQNLRREGNEVANEP"
     gene            39666..40625
                     /locus_tag="PK105_01030"
     CDS             39666..40625
                     /locus_tag="PK105_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOU60316.1"
                     /translation="MSLDSRISKLLENWPAKVISLVMAIFIMFLYNLTRLEQRVIIVP
                     LNISEGHSYVPATEYPKTVRVIIRGDRDQIYRVRESDIVASLDLARYGSEGVFRVPVK
                     LERRGDALNIDPLELRADPSEIPISFERLAAKRVAITPTFKGFLEQGYVLISYEILPS
                     EISIEGPTTLVSSIKDISTDIIELSGKTSDFSLNVPLVKPSELIRLVDTSTVKFSAKI
                     QMQERRLTLKNVPIFINNLDPSLIISDPLPSGNITLVPHPGQDETLANNASLQADFKG
                     IVKPGLYSIPLTVSAPEGFEVEAYEPLALNVRLHGAPVLPAFH"
     gene            40645..41022
                     /locus_tag="PK105_01035"
     CDS             40645..41022
                     /locus_tag="PK105_01035"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOU60317.1"
                     /translation="MISGIGIDIVHVDRIRRWMNEPGILKRFFHPAEIETASLRKKGA
                     ALSLAARFAAKEAFGKALGSGLANFALKEVAVVNDDLGRPIMRLEGNARREFERHGGG
                     KIHISMTHEGDNAVAIVILEKGS"
     gene            41026..41865
                     /gene="truA"
                     /locus_tag="PK105_01040"
     CDS             41026..41865
                     /gene="truA"
                     /locus_tag="PK105_01040"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOU60318.1"
                     /translation="MAEHIHAQENPFCAPKPSLRTIRLVIAYDGSDFSGWQKQKNGRS
                     VQEELEKALLIMHKHEVRVIGSARTDAGVHAMGQVAGFHTDIASIPADRFVPALNRLL
                     PRDIRVLSSEEVKPDFHARFDASLRRYRYFMSCGMAPDPFVLRYTHFSPHYPSLSILN
                     SMAFVVLGEHDFSTFASAQDKSESRFRFISESVFFLERGLFVYQIAGNAFLWRQVRSL
                     VGTFLELEKKAKNPCEAEVMMREVLESKDRSKAGPTAPACGLFLWNIEYGERVHGHPA
                     KAV"
     gene            41840..>42826
                     /locus_tag="PK105_01045"
     CDS             41840..>42826
                     /locus_tag="PK105_01045"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU60319.1"
                     /translation="MAIPRKQCNRKEDQKTELVSPVWEGTANLSVAIRADRFLSEIIK
                     LTTRSQLKARNARLFCNGVEVKLSHKLKNGDHLRLEWNEAISEQLEPEPIPLEIIYRD
                     ADVYVIDKPQGMVTHPAAGNWHGTLANAVLWLENNVRQDNQIKDSSSITSPTRAGIVH
                     RLDKDTSGVIIVARSASVQEFLSSQFREHQARKEYFAIVRGMPPEPKGRIDTWIARSP
                     RDRKKFAVSEPGRGKHALTLYRVCKVWQVNCNIGYRSVGVASKATKPSKYSLLALYPR
                     TGRTHQLRVHCADMGCPILGDPIYGKKDEAFPEATLMLHAHRLKIRLPSRSEP"
BASE COUNT        11253 a         9784 c         9994 g        11785 t
ORIGIN      
        1 ctccggcttg acaggccggc gcgataacca gcttcgctac gcccccttgt catggctgac
       61 ggcatggtac tatataggca cccttgtttt gtgtcaatag gacgcattgc ctcatcgaaa
      121 gtctaaccga aacgatgccg atgcctcata ataagacctg gtccatattg aaccattgtg
      181 ccggaagttg aataaagcgg ctgaatggct ctttcgcgtc cgcaagcaga aagttaatgg
      241 cttattttcg gccagaaaac tttttaggcg attcgtagga tataagaata gcgcctgtct
      301 tttgatcata ttccatttct tttttgcgtt ttttggatga actggtgctt gcaggtatct
      361 ttgttgctat gcgcaagaga gcaccagact ctggcaatgc ctcagattgt ccggtcgaaa
      421 tcagagtata tcctaagtga gagcaagcat atttaccgac tctcgaatga gaaagcacaa
      481 ttggttcgga cacaattcgg caggcgagtt catcgtatcc ctcatttgcc cctgtcggca
      541 agggcaggtt tccagggtaa cgactctttt gcttcttatt ctgatccgag ggcaaaatcg
      601 aggatgggca tcgctttacg taaaggtagc taaaagacaa tttatctttg gccagcccgg
      661 cttcttgtga tagcctataa agccatttag gagcatagct tgtgggcatg gagaaatgac
      721 accaaggata ttttactcgt ggagcgggca ttcggacagg cgtcaagaac ctatttctat
      781 gattgctgga ctcgtcgggg agctgcttga ttatatattc acttttttgt cgtgcaacat
      841 ttgctttgct ctcaatagaa tcaaaacgag ggattccttt tttgattatg tttttgggat
      901 tttctttaat tagatattct tgtccactct tgaaaattcc aggcatggga caagccgagg
      961 tgtgtggaca aggagccaaa atctcgcaat tatcccgaat agcaacggcg cgcagagcac
     1021 tcaaaaaccc tccgcatcta ggctcgccag gttccaggat cagcaatgag ccatcattct
     1081 tgcagtattg cataatagat cgatatagag cgtcagcgtt ttcgaaaagc gtttctttgc
     1141 ttttccagaa gaattcattg aagacattta ttgcacagaa gaggtcggcc ttttccctga
     1201 taggttcccc gaacctcagg tgtctcagtt cgattttcca tggaggcaat tctccagaat
     1261 gttccgcggc gagtatttcc agaatggttt ttccagtctc catcatgcga ggtgctcgat
     1321 ccacacagat gatcgtcaga gggagctttc tcaattcagg cttggagcaa tagagcgata
     1381 agagcaaggt cagcgggccc gaacccatat ctgttatagt agcatttggc ttaagttcaa
     1441 caggaagatc cgtcaaaagg gaaacgagtc gtacaaggtt ccagggcatg aaatagcgca
     1501 aataggccga aaaaagggta ggattgttga gatagtcgct tgcgcgctgg gctcgtccca
     1561 ttgtcagtag tcgccaaaga ctccgaatgc tgtagcgaag ctctgcgcga tgctggaagg
     1621 aaatgggatt tgcttcttcg atacagcgtg gaattttatc caatgctgag gccgcatctt
     1681 cgctaatacg gaaattatag agaagagtat gttcaatcat gggcgaataa ttgagcaatc
     1741 aaggacttct tcattccaga ttcaggatta cttttccgga ctctccggaa agcatagcct
     1801 caaagccttt ctcgaagtcg tcgaagcgaa atctatgggt tattacaggg cttatatcca
     1861 gaccagatag cagcatggtt tgcattttgt accaggtttc aaagatttct cgtccgtata
     1921 cacctttaat gaaaagcccc ttgaatatga ctctcgacca ttcgatacca gctccatccg
     1981 gaagaagtcc cagcagagcc attcgtcctc cattatacat gttttcgatc atttgctcga
     2041 gggctttggg gttgcctgac atttccatgc cgatatcgaa accgccgacc attccaagac
     2101 ttttctgtac ttcttttaag tcttccttat cgacatttac aacgcgcgtt gcgcctagtt
     2161 tttttgccag ctcgagccgc catggattta catcggtcac cactacgttt ctggctccaa
     2221 catggcgcgc tatagcagcg gccatgcagc ctatggggcc tgctccagtg atcaatacat
     2281 cttcaccagt catatcgaaa gagagtgcag tatgagtagc gttgccgaag ggatcaaaaa
     2341 tagccgcaat atcatcttga atggcttcat gcacatgcca tgcgttctgt gccgggacag
     2401 ccacatattc cgcaaagcaa ccatctcgat ttactccgat tccaatcgta ttggggcaga
     2461 gatgatgctt accagcgcga catgcgcggc aggtaccgca ccatatatgg ccctcggcgg
     2521 aaacgcgttc tccgatttta tagtcttgta cggatctacc cttatctaca atctcaccca
     2581 caaattcatg acctacaatc tgcccaatat gtattgtacg ctgagcccaa gtattccatt
     2641 tccatatatg cacatcagtt ccacagattg ctgttttctt gacctttatc agcagatcgt
     2701 tatctccaat atggggtata gggacttctt ccatccagag tccgggttcg gcgtttctct
     2761 tgacaagcgc tttcatattg ctcctttttg actgtgcgca tttcttcccc cattattttg
     2821 gatctatata ttcggttttt tataggaatg caaagcgcat aaatcaaatt ctatcgtaag
     2881 tatactacca aatatcgaaa tgagaaatgc agtaatcaaa atcagtcttc gacagatcca
     2941 ttccatactg atacgaacct tgtgtatgac aatgaagcag aaattagtgc tataataaga
     3001 aaaagagagc ggctcctact accgcaacag ccgcgccgag tacttccaga taatggactt
     3061 tttgcttgag aatcagaacc gatggcacaa tgataaccac gggagtcaga gccataagtg
     3121 tgcttgtagc ccctacctgc gtatgttgca tggagaacag agagagggcc acgcccagaa
     3181 agggaccaaa aatagagcct tttaatgttg agcgaagggc ctcctttatt ctgggcatct
     3241 caacaaagac aaatctaccc tgtccgatga ggagagcgtt tattgcaaat ccgaaaatcg
     3301 caacgaatgc gcgaatctgc gttccggaga ttggactata gttgcccaag cctgctttgc
     3361 tgagcaccat tcctcctgct tgaaaaatag acgaaaggaa gccgtatagg agcccctttg
     3421 cggaaagttt ttctttatta ttctttttgt tctggcgaga acgggtggtg acaacaatta
     3481 gaatgccggc aacggtcact gccatgccga tgagtcgatg tacttgcatc cgctcgctta
     3541 tgaagataaa ggcaaagaga gccgtaaaaa taggcgtgag cgattggaaa ataacagtaa
     3601 tccttgaacc tatcagtata tatgcattga aaagaaaaaa gtcggaaatt acaaagccaa
     3661 cgattcccga caagcctaag agtatccatg ttcttagagg tgcatcataa ggaaaaggca
     3721 cattccgctc gaccatcccg gaaattccaa ggaacaaaaa cgcgaaaacc accttccaga
     3781 aattgacggc caaagctccg actttcctgg aggcagtctc gaaaaaaata gccgaaaaag
     3841 cccaacacac cgcggttcca aatgcggcaa gaagcgcaaa tctctccatt gctcgatatt
     3901 ctatctaaag gactctattt ataaaagggg atataatgct ggcgaccaaa tgttattcat
     3961 aagatagagc acgttccgta ttttgtttga ctcgaggact aacttggatt atgattttta
     4021 agagggaggt gtaatatggc ggttatagca atttctcgcg agctggcatc ttatggcgaa
     4081 gaaacagctc aagagctttc aaagatcaat ggatataaga ttgttgacaa ggagcatatc
     4141 gaagctgctc tttcagcgat gggaatcgat gttgagaaac aggctaggta cgacgagaag
     4201 aatccaggct tttgggcttc tctttctcag cagagggacg aatacctgca ttttctcacc
     4261 caaaccgtgt atgatactgc gctggaaaac aattgcatca ttattggcag aggggctcat
     4321 ataattctac gcggtatcca gaatctaata agcatcagga taggtgcttc gaagacggtg
     4381 agaatcgaga gggtgagcaa agcgcagaat atagattcac gccatgcgct tcagataatc
     4441 gagagcagcg atcacgatcg agcaggcttc cataagtact tcttctccgt ggattggtac
     4501 aatccttcgg aatatgacat gaccctaaca accgatcgct tcgatccaat ccacgctgct
     4561 tctgcgatcg atgcttttag agtgtccttt ataaccgaag atcgagaaaa agaagctcag
     4621 gcaaagctaa gggatctatt gcttggagct aagatagtca cagagatcac ctacaatcgc
     4681 aaaatcccta ttcatttttt ggaggcagcg gtcgagcgag gcgttgtggt gcttcatgga
     4741 gtagccaaca gtcgcgctac agtggaaagt gctgtcgcag cggctaatct tgtgccgggt
     4801 gtcaaacaga tcgagagcgc aattcagctc gtccaggaat ttacagttat accatgatcg
     4861 atacataaga aataggattc ggtattcgat ttctcgtaag gatggtgtgt ggagtatctg
     4921 acatcatttc atgctattct ggaaactctc aaagctggaa attcgcggtg cttcctctat
     4981 ataaccgaaa actctcagcg ccgaggtcca cgaatacgtg agattctcga tgaagcgcta
     5041 tcgcagaaca tttcaatcca atatgtgcct gaatctaggc tcgatgaaat gtcaaaagat
     5101 caacgcggag ctattctcgc gctagaggaa gtctttaaga agaagccttc tctgaaggct
     5161 ctgtgcgatc gagtttctga accaaggcca agccttatct tagttctcga tcatatagaa
     5221 gatcctcaaa atctaggtgc tatcctgaga tcagctgacg ccttcggtgt agatgccgtt
     5281 attatgccac agcggcgtgc ttctccgatc tccaatgcag ctgctagggc atccgctggc
     5341 gctatcgcct gggttcccat attacgattg agcaatctca gatcagcggt cgatactctc
     5401 aaaaaggcag gttattgggt ctacgccgca gacatggatg gcaagccgct tacgagcgct
     5461 ggtatagctc caaaatccgt gattattctt ggcaatgaag ggagcggagt ctcgcaaatc
     5521 cttagaaagg tagctgacga tatcgtttct attcctatgc agggtcatgt cgaatcattg
     5581 aatgtctctg tatctgcggc aattctcatg tatgagtttt ggcgctctaa ctctggtgcc
     5641 tcgagtattt gatatgaaca tttgttgatg ctgatataga tcatttgatt ttggcaaaat
     5701 taacatgcct gctattcata caagtccggt ttccagaggg gatatctata gtctgtaaag
     5761 ctgatcagat atcccagata tccctgaatt tatgctcgcg ttcgcagtac ttacagacga
     5821 atgtagtctc acccttgcgt atgaattcgg tgggaacacc ctcgtgttgt gctggagaag
     5881 agatacagtt ttcgttcttg caggagatct cgtcgaagtt gtatatacga ggcggcatag
     5941 agaggcgata tttcttggct gcgtgagcat ggcgtatgag attgagcgta cagcccggag
     6001 cgatcgctgc gagctttttc aggtcttttt caccaaagct tattatgtcc ggaagagaaa
     6061 taatgccttt atagattcct tcgcctcggt tggaatgata gacaccatgg ctggaacgca
     6121 catctagctt gaggattttt cttacggcat cgatagtatc ccagatctcg cctatggaag
     6181 ctccccttgc tatatgatct atgactattc cttcttcaac aggcttaatt cctaccttat
     6241 actcaggttt agaggtctcc tggatcgatg cttcatgcac aaagtcgtcg atgaattcgc
     6301 gtggctttgc atgttcgcct tcaaaatctc ctccaatacg gccagagagc atggcaatct
     6361 cggttatgcg cgtccaatag ccattgatgg attggccatc ccagccattt agaggcaaat
     6421 catcgaggaa aaatggtatg gttggataat tgcgatctcg cggcagaggg tgatagaatc
     6481 ggcagttttc gggcaatcgt cctagatagt ctttcttgaa agttaccgat tggcgcaagt
     6541 atggagctcg ctcgagaacg gcttcgccca ttcgctccaa ttgaaggcga gtaaagtacc
     6601 aaataggtgc tatattgtcc tgcgcgagat attcatctat cgattcgaaa atattgacct
     6661 ggaaaccact agccctcatt ttatccacat aatatgaagg catggataac aatgagggcg
     6721 ctacgagatc tacttcgact tctttgaata ttcttaagcc atccgccttg gaatgcacgg
     6781 tccttccatg atagaggtcg cccaccatcg ctatgtgtat atgctcttct ttccaattga
     6841 gttgttcgag aaaagagaat tcgtctaaga actcttgggt agggtgctcg tgtttgccat
     6901 cgccggcatt tataaaagaa ggcttgagtt tttcggttat atgggcatat tcctcaaatg
     6961 cctgatctag ccaggtgcag acgccttcga gaggggtgcg aagtatgaaa caggactcat
     7021 tggaatagcc aaagagcatc ttgatggcat cggttataga ttcgttttta ttaaaggagc
     7081 ttgtggcggc gtcaaagacg ttgactcgag cgcccagaaa tttgcctgca tttctgaagg
     7141 attcgcgagt ccgcgtggaa ttttccataa aaataaggta cacttgataa tccagatcat
     7201 tgatccggaa tttttctgca tcgtggccac ctcgcgcagt ctcttttaac tctctagcct
     7261 tgcggtatag atagagttgt tcatctaacg atagatcatt taccaccgaa atcgatctgc
     7321 ccttaaaagg accattacgg gccatgctta gcctccttta taagacgagc ggctgcttcg
     7381 agccttcccg tctgaggata gaagtcatag agaatgatat tctcgagttg ccagcctttt
     7441 tgaaaaagat ctcgcagatc gcgtgcaagg cttgcaagat cgcagcttat ataaagaagc
     7501 attggagcct caacaaaaga aagccatgtg cgcacttttg gcgtcattcc ggttcgcggt
     7561 ggatcggcaa ggatacaatc aaaggagagg ctagaccgag cagcttctat ccaattctcc
     7621 accgtggtgc atgaaaactc tatctttgta gctgctgatt cgaggttcat gcgtgcggct
     7681 tcgatggaag tagcctcact ctcgacgcaa tcgatagctt cgaatctgtc cgccaggaat
     7741 aatgagaaca ttccagcacc actgtaaagg tcgagagctc gcttgccgta caatgagatt
     7801 acattctctg caattaatct ttcgagcatg aagatattgc tctgaaaaaa gcccgcaagc
     7861 gggaacctta gttctttgcc agcgaccttt gcctttgcaa agttgtctct tccttcgaga
     7921 tatattcgat tctcctggcc aaaaaccaca aatcgaggtc tgtcgccaat aagggcggaa
     7981 agggcacgat atggatgggc ttttcggttt tcggagacta gccagcgctc tataatcgga
     8041 accagggcag ggcaatgaga tgtccttacg ctctcgtgtg agccagtctt tgcaaagccc
     8101 aggccaccat ctccgcaggc atggatctgg gcacgattgc gacagtggta gccttctcct
     8161 attatgatct gaagctctcc gggatcaaag cctcctatcc gtaaaaaggt atccagggct
     8221 gcttgttttt taagtaatgc ctgatgttca tagccgacat gctgaagact gcaaccaccg
     8281 cagtcgccaa aatgatgaca aggtggttcc acacgatagg gtgaggattc ttttaggtcg
     8341 atgatcctgg ctcgcgcaaa gcccgaatgt tcctctatta tttctgcaag tactgtctcg
     8401 cctggaattg tataaggcaa gaagatcgct tttccttcgg aaaaagcgat accctcaccg
     8461 tttgaagaga gtttttcaac cctaagggtg agtgtatcgg cgatcatgaa tctgcattaa
     8521 tctatcgaat tgcggatttt ttcgcaacag ggtatagtga gaacatgagc atcaatattc
     8581 gttctcttgg agccgcggag gaagtcactg ggtcgaagca tcttctcgaa gtcgactcta
     8641 ccagaattct catagactgc ggcgcttttc agggaaaacg ggcgaaagcg gatgccaaga
     8701 atagggcttt gctcggagat gatatagagg cttcatccat cgatgccctt gttcttactc
     8761 atgcgcatta cgatcattgt gggcttgtgc cataccttgt aaaaagaggt ttcaccggca
     8821 atatctacgc tacttcggct actagagatc tcgcaaactt gatcatgacc gactctgccc
     8881 atattcaagc gagagatgcg gattatcttt ctaggcaggc agaaaaaaga catgagcaat
     8941 tcgactggaa gcctttatat gataacttcg atgtcatccg ggcaatggat cagtttgtaa
     9001 cggtcagcta tcatcgtcct atctctatag ctgatggagt acaaatagaa tttttcgatg
     9061 ccggtcatat tctaggatcg gcttttgctc gaattaccgc aaaggataag gctgggaatg
     9121 tagctattct tgggttttcc ggagacttag gccgcaaagg caagcccata atcaaggacc
     9181 ccgagtgcct caatgggata gattttctta ttctcgacag tacatatgga gatcgtctcc
     9241 atgaatccac agcagattcc cttctacacc tcgaagatgt tgtaaatagg accgctaaga
     9301 agaggggcaa gattataatt ccagcctttg cagtggagcg cacgcaggag ctgatcttta
     9361 gccttcatct tctgcatgat cagggtagga tacccgatat tccgatatgg gtggattcgc
     9421 ccatggcttt caatgctaca agtatatttg ttatacatcc ggaatgctac gataaggaaa
     9481 cctacgatct ttttacagcc cacgccaaga atccctttgg ttttgcagct ctcaattttt
     9541 cccaatctgt ggaggactct aaggctctca atcaggctaa aggtccactt ataatcattt
     9601 ctgcagatgg tatgtgcgaa tttggacgga tccagcatca tctgatgcat ggactcggtg
     9661 attctgcgaa caccgtgctc attgtggggt acatggccga ggggacatta ggcaggcgtc
     9721 tcagagatgg tgcgaaagaa gtgcgaattc aacaggattg gtaccaagta cgtgcaaata
     9781 tcgaagaaat cgatgctttt tctgctcatg ccgattggaa agagaccata gaatggctag
     9841 agtgtatcga taagaagcgc ttacagagaa cctttcttgt gcatggtgaa caagaagcac
     9901 tgatggcaat gcaaggccat ctgatagatg ctgacctaaa ggacatcgag attgtcaaaa
     9961 tgggtgtttt ctacaagata gcctaaagac agcctaaatg gtatcgagct tagcaactct
    10021 ttcagcgtga cgccctttcc ggaattccgc atgcaggaag acacttaaga gttcttcgac
    10081 aggctcttta taatctactc ggccgccaaa agcgaggaaa ttggcattat tgtgctcctt
    10141 ggccatgcga gcggtatagc tatcgtgtat gagtgcgcaa cgtataccct tgaatctatt
    10201 ggcggctatc gatatgccaa tgcctgtacc gcagcatact atgcccaagt cataactacc
    10261 cttgaaatat tccgttacgg ctaaggctgc ttgatctgga taatcgactc gatcttcggt
    10321 atcgacacca agattgacaa ccacgtgacc ctgggtttcg agccatcgca aaatattgaa
    10381 ctttagcgcc acggcgccat gatcgttggc tatcaagatt ttcatgagga tagagtaatt
    10441 agcaaggcta atagggtcaa gccggaccac ctacatcaga ttgccgataa agcctagagt
    10501 tcagggaata tagtcttgag ttttatcact gtttcaggat tgcgctttga tggggctgtc
    10561 atgatgccac ccttcatggc agtatggcag ctacactcag tatctttgct taatgcagga
    10621 acaatatcca gtattaaagc cttggcatgt gctgagtttg cattgaggtt ctggacgacc
    10681 atatcgaccg tcacatctgc gtggtccatt ttccagcagt cataatctgt cgccatacag
    10741 atcattgcat aacaaatccc tgcttcgcga gctagttttg cttcgggcag cacgctcata
    10801 ttgataatgc cagcgcccca tgaacggtaa agctggcttt ctgctcttgt tgagaaggct
    10861 ggcccctcca tgcatatgag cgtttcatcg gtatgaagac gcaaacctcg cgccagaatt
    10921 agtggctcta tgatagagca aaggcgtgtg caaaaagggt ccgcaaagct caaatgagca
    10981 acaatacctt ctccgaagaa gctggagccc ggcctgtcct tggttctatc tatgatctgg
    11041 gagggcaata cgaaatctcg tggatgaata tcttccttga ggctcccaac cgcagagaat
    11101 gctacgatct cacggactcc gagcatcttt aaggctgcaa tattggctct cgagttaact
    11161 tcagtgggta gaagaaaatg cccttttcca tggcgaggca gaaaagctgt cctgacacct
    11221 ccgatcttag caattgtgat aaggtcggag ggagatcccc aaggagtatc cactttgatt
    11281 tcttccacca cctctgctcc ctcgatttca tacaagcccg atccgccaat tatgcctacc
    11341 tttgcttttt ccatgccttt tccctctctt tattgctata ttcctttatt acaaggagat
    11401 tgcttatatt tctttcgatc ccaaagcaga caattccaaa gcagacaatg cggcgccaag
    11461 agcaccacag agttctggct tttcagagac gatgagtcgt tcgcccagct taacttcgag
    11521 ttctctaacc acaccggtgt ttctggctac gcctcctgtc atcatatatg gtgcctggca
    11581 tccagtacgg ctgatcattg atgtgattct agaagcgatc gatttattga gcccgtgaac
    11641 tatgtctgca attgagcaat tgtccgcgat gagcgagaca acttcggatt ccgcgaaaac
    11701 agtgcacata ctcgagatcg taaggtcgtt tttccaggcc aggccagtgc gcaccataat
    11761 gtcgatatcg agctcaaggg tgcgggccat catctctagg aaacggccag tgcctgcggc
    11821 gcatttgtca ttcatcacaa aattgacgac tctgccttca ttgtctatgc ggattatctt
    11881 gctatcttgt cctcctatat ccactattgt gcgaatagtg gggtcaagaa aaaatgcgcc
    11941 ctttgcatga caagttatct cggttttact atctgttgcg aatgcaatat tgtttcgccc
    12001 atagcctgta gaaaaaaccg atgccaggtc acacggttca acgccgagct gtctacatgt
    12061 ctcctgtagg actttccgtg cgctaacctg tgtcttagcg ccagtagata ctatgctcga
    12121 agcaaggatt ttactgttcc gatcgatgat aaccatcttt gtcgtggttg agccgctatc
    12181 gattccccca aagaaggcag gttgacttgc ctgtcttgcc ttgattttca gtggcttttc
    12241 gaggctaagg tgcaaatttt cctggaaggc ttcgaggcga gtggaaagct ggccttcgga
    12301 ttgaggcgta aaatcggatt cgatctttag tataggcagg cgcacttctc tttttacgcg
    12361 cgcataatcg aaactataat agtcgcaaaa cttgacggta ttatagatta ttccgcaaag
    12421 attctcgttt tcccaaagcg ctttacgcgg ctcgatattt gccatccgca tgcacgggat
    12481 aaaatgcaag agctcttttg cataccaatc cataagatct tcgaagctca ttctctcggc
    12541 accgggagcc agagggctga gataccggtt tccgccgcag gtggcatcga tcactagata
    12601 gggcacttct tctttgatgg tggaaagaag ttcttggctt acgcgcgctc ctattatcgc
    12661 gatgaacgga gtcgcaggat cgagtttaga atgagtccca gaggcagact caaacgaagc
    12721 cttgaaagca acctcagaag aagcccggca tgcttcaaga aatcgccgtc gatcaaaaga
    12781 gacaggatga tgttccgtgt agagtcgagt aaggcgaaaa agttcctttt tgatcttttc
    12841 ttgcgcgcag cccttatcta tgtgcggaag atcaaataaa aacagaaatc gatgaacttg
    12901 ctgtgcaacc aacacatcat atgccctgcg aatggaatcg cagcaattta ctaatacaag
    12961 ctcgtccagc tcagagctat gttgcaggaa ggcctttgca tggcagcaaa ggtttggatg
    13021 agtccatttc tctgcatatt cgaaatccgg cacttcatgg ttgagtagct ccgtggtctc
    13081 tcctaaggca tacaagagct cgataggcgt atatttgcag gtgtaaccaa tcatcgcctt
    13141 gcctctaact gttccagaaa agcacttatc cgagttagca tctgaccatc ggctacattg
    13201 cgtggatcgc agccatctcc gtccagcacc agagtaggga agccatgggc ttcaaggctt
    13261 atttttgcca attgcgacat gcccatagtc tgcttgcatc cccattgacc gaacacaatt
    13321 acaccatcgg catgcagttt ttccgcgtag gaaattgctg catcgatacg cctgtgtgcg
    13381 cccccattgt tgatattata tacaaggcgc ttggccatgc tttcgtatgg tttttccggg
    13441 tccatatcga tcagtgtatc atatgtcata tcgcttgcca ggatttcgaa atgttcattg
    13501 ttttcaagaa gaactctaat agaatgctgc caatttggaa gagtatggag ccataggatg
    13561 cgtttttttc ttcgatcaag agaggccttg tgcgctaatt cttctaaagc aatggcatat
    13621 tgctcagact ccggcattcc caggaaagca tgcaaggctg caagagaaaa caattcgccg
    13681 gtcatggaaa tgggaagcga gatttctgcg cgaaggtcca ggtattttcg gtatgctttt
    13741 atggtgcgat tagccctatc gatggtgtct tgaagctttg attcggccat tgggcagtgc
    13801 gatagatcct caagaagaga agtaatattt ttgagctggg aagatacata gtgcacagac
    13861 tccttatcga ccgtatgagg cacgtcgatt atgatgtgag gagtatgata ttcatcggca
    13921 agtaatctaa aggagagctg attggcatcg caggccaaag tagtatttgc gataagtagt
    13981 ggtttaggaa gtactcctgt ccatgctgtg ccgatcatga tcttatgata agagcacaag
    14041 ctttcggata tgcctatagc ttccgcggat tctgcgaata tctctccaca gcccgaattt
    14101 gcaatgtata ctgacaaagc ttccggaaac acaggtacaa tctccattgc gtatagtaac
    14161 tcgcatggaa aaaagatatt gaccatagcg ctatgcgatg aatccctaaa agagcggctc
    14221 atgtttttgc ttattacgga attcatgcga gcatagatcc ctctaagtcc tctacctctg
    14281 aaatatgcat ccttccaact actgagtgca taaactgtgc ataatagact acgcgcacgt
    14341 tgaggatttt cgaggacctg gttcgaaacg actctgccga atctatgaac aatggaattc
    14401 atgccatagt atcatagccg agatcgggaa tcgaacccga cacctgcgca ttacgagtgc
    14461 ctttttttga tattcagcag tattttgctg tattcattat catatatatt ttagagcgtt
    14521 tttgcttgtn nnnnnnnnna cacctgcgca ttacgagtgc gccgctctgc cagatgagct
    14581 atctcggctt ggtcagaacg agcgtactat agcagaagca ggccataata atcaaggcaa
    14641 ggttctttat tgaattgctt tctcgcgcct aggatctatc cctggttttt ggatgatctg
    14701 cttttgccaa aagtatataa acgatttgag acactcgaga cgctattgat ggtttgtctt
    14761 gccaattcgg ctgctcccag cactccagct ttgctcccga gctctgctct ggccagaaaa
    14821 agccttattg ctgcggtgct gttcaaggcc tggatatatc cgcccagaag ctccctcata
    14881 cgctcgctga ttaaatcgag caggccttcg cgaagaccga ttcctccacc aactatgatt
    14941 cgatccggtg cgacggtgag agcaatatta gtgagcgcat atgcaaggta ttcggcttca
    15001 agcttccaag cagggtgttc tatcgacatg ttttctggcg cttctcccca gcgagcagcg
    15061 attgccggac cactggcgag cccttccagg caatcgccat ggtggataca aagacccttg
    15121 aattcatctg gtccagagtc tgctgtcctg gctcttggaa cgcgcatatg cccaatctca
    15181 ggatggaagg atgcgcgcat aggaatacca tcgattataa tacctgcgcc gattcccgtt
    15241 cctatggtga tatatacaca gcaatgcgtc cctagtccgg ctccccattt ccattcgcca
    15301 aggccagcgg cagcgacatc ggtttcgagc ttgataggca ctcccagttt tctttgcaat
    15361 ctctgcgcaa tatcgatatc gctccatccc ggttttggtg tcttgagaag ataaccccag
    15421 agaggactct cgattctgca ttctattgga ccgaacattc caaggccaac gcattcaatt
    15481 tctgagtcct tgaagaatcg gacaaccgca tccagggttg gctctggatc tgtagtcggg
    15541 atacgctcta ttcttagaat tcgaggagtc ttttctggta tatttggatg atcgaattgc
    15601 agaacggcac agacgaattt tgttccaccg ccttcaattg ctcccacaat catatttttg
    15661 atcctatttc tgattatcga tttcttcagg caagcactct gagcataaat cttcttcgaa
    15721 aggcttcgat atctttataa aatttggagg atttcgaaaa atagccttta tataaaagaa
    15781 gaaatcctca ttagcaccat aaaagtcaac tatgtgtcct atctggctat atcccgagtt
    15841 ttccagagtg ttttctccca ggctatctct ttttctggtt gatgtttcaa atcggatcac
    15901 cgcaaaggcc attttcttca ggagttcttg atcgatcatc tcaaaaagat gctggatagc
    15961 gtttgtggat cgataatcgc tatcaaggac aacaaaacgg atatcgaatg tgaagttacg
    16021 ccctgctgcc ttatggatga ttgcaaaagc gacaagagtc tttccatcgc gcagctccag
    16081 aagtgtataa ttcttgcctg gcgcttgttg ccaatccttg aggatttcca gcatgaggtc
    16141 tatttcaacg cccttaaatg ctcctgagtt gcctgcaacc tgctcatatt ctttaaaatc
    16201 caatgaagaa aacattcatc tgcctttttt ctgttgcatt ttttccaaat gttggtgaat
    16261 aactataaga tatgagcgca attgtgtaaa cccatattta tacagcagta tatgatcagt
    16321 tgagcctcat ttctgtccac aaaatacggt ttttcataaa aaggcgcatt atattcaata
    16381 gagcttaaag cgccgaaagt tacgattatt gggaatattt atatataata tcgaaacaat
    16441 agtgctgatt ctttctattc ttaggtcttc atcctctaga agctaatcag cccagccttg
    16501 cacaagattg agcatggtat ccaccgagag tgccatttct gagagagaag cccattcaaa
    16561 gcgactatga taattgtagc cacctgtaaa gaggtttggg gttgggatcc ccatttcggt
    16621 caagcgagag ccatctgtgc ctcctcggat tggcttcatt atgggttcga tatttagaac
    16681 ctttatggca ttcttgagtc tttcgagcac ttcagggtgc ttatcgagtt tttctttcat
    16741 gttgagatac tgttgagtga cattgagttc tatgtggcct tgaggaaact gggcttcgac
    16801 tgccgcagca aaggctttca gtgcggctat tctctcctgc atgcgagaag ccgaaaaatc
    16861 tcgaagatat acttcagtcc atgcatgctc tagccctcca gacacttcga tggggcagta
    16921 gtagccaaac catccatcgg tagcttcggg gctctctgaa cgcggcaaca tgtctataaa
    16981 gcttgccgcc atggcaactg cattagcgag tcttccgcga gcggcgccaa tatgaatcac
    17041 cttgccaaag aaatctgctt ttacggcata tgcgttaaaa cactcggcct ctatttcggg
    17101 tgcggcacca ccatcgaggg tataacaagc cttggctcgc gctttcccca aaggaaagat
    17161 gtccatcccc ttgcctgtct cttcgtcagg agtaaagtat atatcgatag gcccatgctt
    17221 aagctcagga tgctgaagaa gataagcgac ggccgtcatg atttccgcaa tgccggcttt
    17281 atcatccgct ccaagaagag tagatccatc ggtcaccaca atggtatccc cgacatgctc
    17341 atatagatcg gggaattcgc caggatcaag cagatactgc tcggaaagcc atattccctt
    17401 accatcgtaa ttctttataa cacgaggctt tacaccactt cccgaaacat cgcttgcagt
    17461 gtccatgtga gccataaggc caatacatgg cacgtgttcc ttgcccgggc ttgcaggcag
    17521 atgagcgatc aggtaacaat gcctatcgag ctccagttcc gaaatgccaa gttctctcaa
    17581 ttcttgttcg agttggcgcg caagatccca ttggccttct gttgtcggtg ttttttcagc
    17641 gtggcgattg ctctgagtat cgatcctcac atagctgaga aaacggcgga gcatttcttc
    17701 tgtaaaggaa cggtttaggt aatcgagtat tgctatcata atagttttaa tatgacatct
    17761 gtaatatggt ttgcgcaagg gagtctttac aaatcaagat gaaacaccat gttctttgac
    17821 gcagtgcata ttttgatttg gatagttcta tgggattctt gtctcggttc ttgtggcgga
    17881 gtatattata ataccttatg acaattccaa agtatccaga atttgcacct cttacgctag
    17941 aagcaagaga tattctctat ccagctctca atttgctgcc agatggtata tcggaattca
    18001 ccttttcggg gttatatctt ttccgaaata cctatggcta tgagatttca cagatatcag
    18061 acgaaaaatt tatcatctcc ggtcgctaca agggtagaac ttttttctat attccctgct
    18121 gctttccatc gatggaacaa ttcgatgagc ttatgttgaa tttcgattac atgcgccatc
    18181 tatcggaatg ccaggctgct aaacagagga tcgagctaga aagacgtgga tatttggtgc
    18241 gagaggacag agataatttc gattatctat ataatcggga agacttagct actctcaatg
    18301 gccgcgtata tcacaaaaag cgcaatctag tgaatggctt tgtaagcaat tatgcctgcg
    18361 aacagcgccc tttgactaag gaccgtgccg cagatgcatt gcaggtcctt gaagagtgga
    18421 gagcggcaaa aggggcggat ggagactatg ccgccgctcg tgaggccatt gagtgcttta
    18481 atatcctggg cttgcggggc gcggtttact atatccacca taatcccgta ggctggtgcc
    18541 tcggagaacc tcttgcaaag gggaagatgt ttgccataca tttcgaaaag gcgatcgaca
    18601 gctataaagg catctatcag tttataaatc aagcctttgc ccagtctctg cctaagcact
    18661 atctgctgat caatcgggag caggacctag gagatctggg tcttaggcag gcaaaaatga
    18721 cctataggcc tgtaggtttt gtaaaaaaat acactgtcga gcgtcctggg ctagctaata
    18781 ttgttcccgc accgatcgaa ccccctgcgg agtgcggccc tggtactgtc catgcgtgcg
    18841 atgaagattg atggagctaa gattcatgga agcaaaccca agagcatatt gatcgctcct
    18901 gatccaagaa tgacccagac accattgatt tgagtgaatg cagtaatgcc aaaggacgcc
    18961 agagcaaata tccatggcat aggtatggta ttctgaggca atagggtcca gagcgtcata
    19021 atgatcatcg ccatgctgcc agatcgcaag ctctcatcgg ctaggtcttt ctttataggg
    19081 atatatctgg aaaaggccag cgtaagggac atcgccagaa gtggaaaagc tataacggag
    19141 aaggttgcag ccagtgcgcc gaaaaaacca gccttttgcc aacctaccaa tgtggctgca
    19201 ttgagtgcaa taggtcctgg cgtggactga gctatcgcaa tgagcgagct aaaggcatcc
    19261 acactcatcc agctgggaac tatttcttgc ttgatgaggc ccactaccga ccagcctccc
    19321 ccaaaagcaa ctatacctat cttaaaaaat gctaaagata tttttaggat ttccatataa
    19381 gccttcctag gtatatgacc acaattcctc ctatcaaaat aaaaaaggca gccgatggga
    19441 aaagaacgag taatatagtg agtaacccca cttcgattaa ggctctggtc gtccatttcc
    19501 gattctttgc agtcttccag agcatcgaac ctacaaggcc tggaacgaca gcgccagctc
    19561 ctttcaaaaa gcgcttgaat agctcggtat ctccataaag gctgataaag cctccaacga
    19621 ggataagcgc aataaaaggc ggcagtacaa ccccaagcac cgcggcaatg gctcccttga
    19681 cgcccgctat cttataagaa cagagaaatg aagtactcag agcgatcggg cctggaaaca
    19741 cctgggctcg cgagaaaatt ctataaaatt cttcttcgtc tatccaaccc tgcttcttga
    19801 aggaattgcc tatgacagga acgatcacat acccgcctcc aagcgtaagt gtattgatct
    19861 gtgccaatgt aagaaaaatt cgcaaagggg ttggctccgt cttttttgtg gttttcttag
    19921 caccattttt cctagagggg attttcatcg atcctcctga attatgaatt gacatgtgta
    19981 tcaaaacata tcgaatcgca aattttcaat aggatctcta tatcaggatt agcattcagc
    20041 caggcaatct gtccaaaatg caaatatatg agccctgcgt gaactttttt cttttgaaag
    20101 agttgttggg cagctatgca ataaagccca agttgaagag catacttatg aggcagtaat
    20161 cttcggtcgg ttttcagatc gagtattatg acttcttcat cactttgggc tacgaagtcc
    20221 attcttcctt cgataatcca tctccccagt ccaagcaaaa agggaaattc gcaactgtat
    20281 ttcttttcta ggacaagagt tgagaatgta ggggcttcaa agagcggggc aagctcggct
    20341 gctgcgtttt ctattataga gcttagatag cgaggatcgg tcttcttggc gatagatgta
    20401 tcgccgaact ctggtgatgt gcttccaagc agctcacgag atagggacgc agaaggccta
    20461 aagttttcca ttgtcttttc agccaaaata tgaacaaaga gttcatgtac taaagaccca
    20521 taggcactga agtcgagaga ttcttgggta ttttgggctt cgagtgaagt ttcatcaagg
    20581 gcttgggctt cagattgagc tcgagactct gctgggctga atatacgttt gcttacaaaa
    20641 tgcccggata gttcgcgcgc atagtccgcg gcaagagact gctgcgcaat tatggcattt
    20701 atgctgctgg ccgtgatacg ccgagaatgg ctcccggcaa cggagtcaga aggcagattt
    20761 gtcatcttag ccggttcgat gaacgatttt tctgtttttg gggccgtctt gcagagagct
    20821 tctttggatt tctgttctga ttcttgtttt tctttctcag agaggagttt tttttgagac
    20881 aattttctgg cacgcaacac gtttgatctg gctccttgta tctctttgtc agagaccagc
    20941 ggctcgaaaa ataactgcaa acccgacaga tgagcaggaa gttgagtttt ttcgctatag
    21001 tctcgtggga tgagatgagc ggtactctga aagagaagtg catggaaact atccctatca
    21061 tagggtttat tttcgggagc ggcagagaag aatataaggt ggtctattgc tcgcgtgcat
    21121 gcaacataca ataggcgctt ggtttcagct tgctccttct ctgtgcgctg ggcttttgcc
    21181 tgagtctgaa gaatatttga gccgctctga attcgatcgt gaaatcctat gtccactgta
    21241 tagaatcgtg ttcttccttt ctcctgagcc gattcaagaa ttccccagag ttcttttccg
    21301 ccatttttgt tggtgcttgc ttcggtccat gggattatca ctatggggaa ctcgagccct
    21361 ttagatttat ggatagtgag caggtggaca ccgtgcgctt cggcttttgg cacggaattg
    21421 gtgtcgaaca aatgcatgtt ctgcatataa tctcgaattg atattaggaa cgcggatatg
    21481 tgcccgccct ctgcctctgt gtttgctgcc agggttcgta tagcattcca atgatccagg
    21541 tatggcaagc gttgttcatc gcagagaatc gaagcctcta gccgtccctc cttccatata
    21601 taggccacaa gctccatgag aggcatgtga tccgattttt ggcgcaactc ctgataaaat
    21661 aagaccattc gttctataga atctcggtca ttggggttca gttttattgg catgtctgcc
    21721 tctagatcag ccagactctt tttctcggtg aggatgctca caaatccatc gttcgaaatg
    21781 cgacacaatg gactgcggag taccgcggct gttgcaaaaa gatccttggc atctatgaga
    21841 aggcgcagga tgtagtaaag atcattgatc ggctcttcag agaagagact agcagcagta
    21901 tcgctgacaa agggaatagc cagcatgcgc aagtattttt cgagctcgta ttggcgtctt
    21961 gttgtgcgca tgagaactgc gatatcgcta tattcggcct ttctaagcga taatttatgt
    22021 tgtttttttt ctgttattgc cagatcctgt gtttcccctt cctttctgat gagtaggggg
    22081 ttcgaggatt caacggcgtc tcgtatccac agagccagac gaaaagccat cgactccttt
    22141 gccgaacgga tgccgcaggc ttcctcttcg gccggtattt tgttcgttat gcaatgatac
    22201 tcgatccgcg aggaaaagcc tttggactct tcgatattag cattctctgt gcttatagtt
    22261 tccatatcct gatacagagc ctcgaaaggc tgcggattct ccggattggc tggtagcata
    22321 acaatgcgaa agacgctgtt gaagaattcg atcagttggt ttgtgcttcg atagtttagg
    22381 ttgagcgaaa gtggagccgc cattagctca ttggcaagcc ccttgaatac gcttacatct
    22441 gcgccacgga agaggtagat agactgtttc tcatctccga caaaaaacag ttttcctctt
    22501 cggatctctt tgccctcttt gctcaagaga aaaaggagtt gtttctgaag ctcgttgtta
    22561 tcctggaact catcgatgag gatatactga aaacgagaag cccagaagat gcgcctgtct
    22621 tcatgattgg acagaatgtc tactgccagg gcgcctagat ccttgtaatt cattatattg
    22681 gctttgcatt tttcttcaca tagcatgtct gcatattcat cgaggcgatc catgatggcg
    22741 taataactcg gcaagaactt ctcaaaatcc acgagctgag agattctgtc tcgaagcgcc
    22801 ttgtttttga gctccttggc aagctccttg attgcggatt ctgtttcatt tcggcccaca
    22861 ctttgaagat ttatggcatt ttccggtttc tccatataat ccgaaaagtc tttcaaacct
    22921 ttaagatcat aagaaaggca tgagactgtt ctagctgcgc gaatagcctg attcgtacct
    22981 tctctgaagc catgtattgc gtaattgcct ggtttttggt tagcgatttc cagaatattg
    23041 agtgcaatat cgtgtatttt ctctgcaatt tcttcagcgg ctttcttgaa ttcctgttct
    23101 ccgattcgag ccgaagcgcg acagatttgc ttgcctttcc aatgcggttc gacatttgcc
    23161 cttccaaaag aggcaaaaag gtcttgaacc acggcttgaa aaccaaaggc aaaaactagg
    23221 ctttcgattc catctctttt ctgattatcg aggacaaatc ggtatgcggt cttttctgca
    23281 atgcgtgcgc acttactctc atcgatctga aaatctggag tatagccata tagaacagca
    23341 gcttgagcca cgatttcgtg gcagaaagag tctagtgttt gaatatgcgt ttgcataaga
    23401 cttctggcga gattcggacg gtcattagca aatgggctta atgcctgttg gactctctgg
    23461 aacatttcgg cagctgcctt acgagtaaag gtgagtgcca atatacgctc tggatccaca
    23521 tttttctgga gcacaagata tagaaaacgg cctaccagag ctcttgtctt gcctgtccct
    23581 gctccagccg taacgatggc tgtatcctcg gtataagcgg cctttttttg aaaatcgtcg
    23641 agcttgagcg cttcaatatc gaaggaatca tggtgcgata tcattatagc tccagaaaat
    23701 accagaatct acaaagcggt ttgaactcac agtcctcaca gaccgcgcga tccagcgcaa
    23761 gatcaagaaa attcccagct ttcacctttg aagaagctac tcgcagcata tcgcggagcg
    23821 cggaacgctg gaaatccgtt tccttgctgt aagtatacgc cgatctcatt ttaggcactg
    23881 taggaggcct gatatatccg acagcttccg ctttttcgat ggaccaatag aaagctcctt
    23941 ccacgcgacc ccaagaggtt tccaccagga gagtataaga aggtacctgg atttcctcga
    24001 gaaaaaatgc actgctgtca tatccgtctt ctttctcttg gttttcgtca taggatgcaa
    24061 gctcttcata gtcagaaggc attacaccga ctctcatttt agatatagat ggtatacgat
    24121 tctttttata atccactatg acataacatt ttatatttcc taagctctca tcttgcctgc
    24181 tgaaaaggca gtcgatcctg ccctcaagga ttgcttcttc ttccgaaaat tcatgctgaa
    24241 gcctcatttc gaacccttcg cgctcatagg ccgaaaacat cgttcgttcg aaattccaaa
    24301 gctcatttag tctgtgaatt gcacgcggga aaagcgcaga caaaataggc tgaagtccac
    24361 tgccatactg gaggagaatt cttttggctt ctatacttag ggctgagttt atagtcttcc
    24421 gaaattcctc atccgtctcg gcattcttaa aattttgcgg aagtgcttgg atggcgcgtc
    24481 tgtaggcgct atgatataag ttgccgatgg ctatggcgct atcgtccctt gaatagacat
    24541 ctaccaggtc gatcttgctt gcaaaccagc gaaagccaca ctgcgatagc tctttgagcg
    24601 aagaagggct aaaaatcgca tacttctttt ccgaatcgaa gctcttagtt gcgttcaaaa
    24661 aactgcgtgc tactgttggc tctataggtt ctggatgaga atagctctct gtagcgcttt
    24721 tttgttcttt agaaattaag atagctcgaa gtccacgctc tgcattgggg tcaatcatta
    24781 ttgcaagagg gaatagcgga taggccgtct cataatcttc aaatcccttg gctgcaaaag
    24841 agaactgggt tggagaaacc cggtgcatat ttatgacttc gtgagcctta tcggtctttt
    24901 cttgctggag aatgcccgca agttccggtg gaagggcgct cttgattttg agcgaagaag
    24961 ccaaaccctc ctgcgaggca cccgcgacat aatgggaaag cactgcaatt cccgctgtgg
    25021 tatcaaagtc gtatacatac actgcattct cttccaccaa aggaatatag ctttttgact
    25081 caagtacagt caaaaagagc tcaaatggcg gaatagagag gtctgtgaaa atgttttcat
    25141 ggtctatcca actctgtagt tcatctagag taagacgcat cgagcgatcg gtctgtatat
    25201 tggctccgaa tccacttgta tcgatccaat gctccagaaa accgattatt tgatcataga
    25261 gcctggagaa agagtctgcc ctggctatgg acaagatgct ttcccagagc ctttgataaa
    25321 tctgagccgt agattcgggg cattggcctg tcccaaaagc gcctttccat agactgtgta
    25381 tgtatgcggc actgggagag ggcgaaggaa ggcgcaatga taaggccgta tctcgcagag
    25441 ctttccaggc ttccgggtcc cgtcctgtaa tattggcaag atcggcaaat gcatcgaggc
    25501 tttccagagt caagccttcg cgagccgcat tctgaattgc catcaatacc ctgccaaatg
    25561 gcgacataga tagtggctta ccccggcgaa tagagactgg aacaccatat tccgcggcaa
    25621 tttgacgaat ccacgcttcc ttttttgcag ttagcccaca tacagatata gcgatatctt
    25681 caggctcgat gccttgttcg atttcgttcg atatttcttc cagtatccat tcgatttcca
    25741 caagcgagga gttgaattga tggtagagca tcggagcctt ttctcctctt tcctgtgtga
    25801 agatatccgg gaaagcaatc ctttctttgg cacttaagga tttctcgtgg ccagtctcct
    25861 cgcctaaaat ccccctgaaa ttgatatttc catcgcccaa ctcttctgaa tctatcagtc
    25921 caaatcggat aaaatcatct ctgagtccat aagctcggac tttcataaac ggtggcaaga
    25981 ccaaagggcg cctatgcgaa tccctaagtc gagcgcgatc aagaaaatcg atgtagtctt
    26041 cagccagtaa tctcatttct tcaaattctt cttttatgaa gcgacatgga gacgcatcta
    26101 gcacagcgct gtctttggtt agcatttcta tttcgaatag atagggcgcg aggcgtacga
    26161 gccttgtaag attcttagtc gagatttcca tagaaggaag caaagtagat agtcttttac
    26221 cgggctttct ttctcctttc tgttctttag aaaggatttg catagcccag atccagcggt
    26281 ggataggctt tagcaccgat agatcatctt gctcattagc ctttgccaca tataacagaa
    26341 aattatcaaa acccaaaaaa cgatttagag ccaccgctct ggatatttcg gaatatacaa
    26401 cggcgcgtgc ccaggaatcg gcggtatctt gagaaggaaa aacaaagaca atattctcat
    26461 cttgcaggtg ttgtgagata tcgcgaccaa gcggagttcc taaaagtgat ttatatccct
    26521 ctgctatttt ggtcgaatct acagattttg gcataactta aatgatcttt cctaaaaagg
    26581 tctttgtctt aagcgcatct ctatgtttgc gctacagatg ccatgagttc gagcattata
    26641 cctgtcctat aacctgcgac aatcgcattc tctttcagta tttcagcaac ttccttccat
    26701 gatggatctt ctttccattc tctttccttg gtttcttctt gaatcgaatc gatccatgca
    26761 aaagcaggca agagacttgc aaaagcctca aaacgctctt tattgaaata ttctgttcca
    26821 cgatactgat ttatattgag agcttcgcgc attaaaggat cggaaaaagc ccaggcacaa
    26881 atttcttgtg cacgcttgtc cggagtcaga gtcgaatcga ttgttctcct tgtgtcaaag
    26941 aaaagtttgt cttgtctcag cgcccaggct attgctatct ctgcgcatat tgagacatgg
    27001 tatttagtgt cgtgactatc gtttctgcga ctcagtccga ctgcagattc agtgagtttt
    27061 tttgcaattc gatattcttt tacaacgcga gaaacttcct cattgagttc atctgcggcg
    27121 aaagctttta ccaaagacct cataaatgaa tagattaaga gaatcagcct atattgctga
    27181 tattccgaat tggaagcact gctgttattg caaagagttg ctgcagcaat gcctgcatcg
    27241 gactctgttg tcccatatag cagcttgagc actgtcttaa gccatgagct tgcagatcgg
    27301 actgagtcgc tgcgggatgc ggcaagagta tcatcactcc cggcaatctc acaaagcctc
    27361 gaaaagaaaa tctccgaaaa aatagtcgct ctttgaatag cttcttcctg cgagagattt
    27421 tgaatcgtct ctatgctgct gaggctgttt atcgcattat ggagcgatcg atagagctcg
    27481 ggctggtcgg cctcaagcaa ggcatcatcc agatccgcta ttcctctcga atcaactatt
    27541 tcaaagagct tagtgtatct gccctcggtg tcgtgcacat tcatgatgtc gatgaaaacc
    27601 ttgttctggt agccttccag agctaatttg aaaccgcggg aagcaatatc ttcgctccgg
    27661 aatatatacc atagtccgga acgaatctca tgcatgatga cataattttt tggagaggaa
    27721 tcgattttga gtgcattggc tagactcgtt gtttttagtt gttttttgcc atcagcggtc
    27781 ttccgggcaa aagcgcaaga ggtatgaatt cgccctaaag tacgctccca gtggttgtta
    27841 tagaatacta gaactcgccg ctctccctgg ccattgcagt aggcgaagac attttcgtcg
    27901 attgttccat catcttgcat ataatcaaat aaataaaagt tttcgacttc tgagaaaagc
    27961 cgacgcattc gaagcagcgg gaaaatttcg cgctcatggc gtgcgatgag atctctatcc
    28021 ggcgtttcat cccaataaga gcgcttatat tccatgccat acttttctgt aaaaccttcg
    28081 atttgaccat gagcgatcat cggtaatcct ggcatagtag ctagcatagt ggcaacaccg
    28141 aagtatttat ctcctttgcc gaattgagca accgccgtct cctcgtctgg gttgctcata
    28201 aaattgacaa aacgctttag aatatcacga tcgaactcct gtgtattctt gatggtcagg
    28261 cgatacaatg agtttttttc gtctttgagc atgttcataa aggctgagtt atatacgcgg
    28321 tgcataccca gggttcgtac aaaataacct tccataagcc agaaagcctc ggcaagcaga
    28381 agcgtatccg aagccttttc cgcgcacagg tctactacct ctcgccagaa ttcatttggc
    28441 attgccttgt caaaggcctc ttcgctcata gcatggtctg agcgcgaggg aattgcgcct
    28501 ccagacccgg gcgccgggta ccataagcgc cttatgtgtt gttttgcgag caccatcgcg
    28561 gcatcgaatc ggattatgtt gaaatgagcc gcaacagaca ggattcgctc ttttaccgcc
    28621 tcgcgcgaag ccgggtttag aaagtctatc tgcgcggtat cattccaggg catgcctgtt
    28681 ccatcgttgc catggtaaat gtaccggact tctccagtat gacgatcgag ccttttgaag
    28741 acgaccgcag catcgccgcg attgtaataa tgatcctcga gccaaatccc aatagatgga
    28801 tctccggaaa gatcgggacc attgaagcta tagctaggga atggacagta tggcagactc
    28861 atgaatagct caggccttgt acgtatccag agcgagtcga tccccgtatg attagggacc
    28921 atatcggccg ccagccttat gccatattgt ccacattgct ctcgcaacct gtccagtgcc
    28981 tcccagcctc cgagctcagg gctgatttca tagtcgaata gggagtaagc cgaagcggct
    29041 gcttctgagt ttccacagag tttttttatt ttttcgcttg ctggacttct ctgccagatt
    29101 ccgataagcc ataatccatt gatccctcgc tcggccatga ttatgagctc ttcctcggga
    29161 atctgatcca ggcgggatat ctctcttgag taagttcttg aaagctggtg taaccagaca
    29221 agggcatttt ttgcaataag cacaagagaa ggcatccagt tcttgtcggg gctgaatttt
    29281 tcatattcat ggaagctgct tctatactga tagggaacag caagccccgg ccctggagga
    29341 aaatgcggtc tggtctcttc gttgatcatg tctattccag caaggaggct gagcttaaaa
    29401 tcgcccaaaa gagttcccca attcttgatt atccattcga gctggtcctt tagcgaagca
    29461 ggtgcgtgcc tggatggttc tcgcagaaaa tttatgaggt ctagagtttt cccgccacca
    29521 taagataagc ctggcaaggt tttgagagcg tcttcgagac gagcaaaaac ctgtaggtaa
    29581 ggagtctttg cagagataga atctggcaat gtggcccctg ccggagagac cccgtcatcg
    29641 aagagtgatc gatatggcca gaatgccgga ttctcgttaa aaagacgaac caaaattagc
    29701 tgctctatcg caagttctag attaggctta ctggagtcgt cgccaacagg actcagggat
    29761 tttagccaat ccttgggttc ggcaagacca tcgtagatgg gagcgggggg aaattctgta
    29821 atcaattcct ggagcacctc ccaaagcgcc ttgttgtctt ctgtggaggc gaacgcttta
    29881 agcgcaattt cttctagcaa cttcgaatat tgctttcgat ataggtcgat gataatcctg
    29941 aaggcttcac cgatcaaaga aagagctctc agctctcctg cagacagctt atatttctga
    30001 gccacaaagc gtgcggatac ggcatcgggc agagagatag ctcccgaagg catccaaagg
    30061 tgcttgggag gaatattttg agagcggaat tctctgagga tctgtaaagc catcgataag
    30121 ctcctttatc aactatacta gtaatttatg ccgcaagcaa cgcaaggaac aggaattcat
    30181 atcggaagat atactaagat cagatcttct gttatgataa caatttgaca aaggaggtcg
    30241 atttgtctag ccaggtcgcc cacgaaaaga gaaagcagga aattctggaa aaagctctcg
    30301 atgtgtttgt agaagaaggt tatgcagata ctactttcca gaagatcgcc gagagatgcg
    30361 ggatcactcg aacgatactc tatctctatt tcgacaacaa aaaacagatc ttcaacgaga
    30421 gtattaagcg ttttctctcc tctctcgaag cggagatagc gcttattgcc cgggatcagt
    30481 acctaacaaa tggtgaaaaa ataaccaaga tcagcgaaat cctggttgaa gcctgcgaga
    30541 gagaatcgaa aatgctctcg gtagtcatgg attatctgct gagactaaaa gcttctggag
    30601 gaaatccaga cgaaaaagta aggcgtcgaa ccgtccgcat gcgtcatatt cttgcagata
    30661 tcgttatcga aggcataaag tgtggtgaat tcaaggaatc ttcttccgta aaaggaacag
    30721 tggagatgtt ctttgcactt gtagaggctg ctgtatttcg tctcgtggta cttgggcgaa
    30781 aggaagcggc aggattacag gaagcgctta ctccttttgt cgataatatg ctgaatccaa
    30841 attttgaacg gaatattgag aacaaagagg actaaagctt cattgattcg ctaagagcaa
    30901 tgctagcgat aaaaaagttc ataattttgt gttccataat agatacttcg gaaatatatc
    30961 taaaggtcac accaggaatg cctttttctg agattctggt tattcccgca ttatatgctg
    31021 caagagcaga gaattcatcg ccatttagat tgatatattc ttgaagatgg cctatcccga
    31081 gtcttgcatt ttgatatgga tcgtagatcg atttttcgct tatcttgcca tataccgatg
    31141 aattgagctg gaaaagacca ctattgagtg tacccgttcg attggtagaa cgagctcttg
    31201 gattgtatcg gctctcgaca taggcaaccg caaaagcaag actaggtcgc atattatgag
    31261 ccacagaatt atccaaaatc gctctagcta cacgttccga attagttata gcagaaaaga
    31321 aatcaaggac ttcatttttt gtcttaggat tagtataaag cttcagaatt ggatcattat
    31381 aattattgat attaccgata tttattgaga acataatact tgatgattta ttatttgatt
    31441 tataaatcga aatggtttcg cataaccttg cggctctaga aataacaaat aataatgcaa
    31501 tacagaaaga agcaataaat acaagcttta tcactctttt tcttttaata ctatttgaat
    31561 tactatcaga ctccattcgg aatatcctcc cgaacctcaa tataattagc gcccataaaa
    31621 aaaagttgtc aagataataa tgccgattta taaaaaatgt caatgcgcca aagcgcgaaa
    31681 atatttatct cataatagaa ttaatattta gctaaattac gataacctcc tatgaatata
    31741 tattgcaatg tgttgatttt ccgatagtat tgtgttttga ttgtgcaaat aaaggagatt
    31801 atagataatg gctcgcgtca agacaaaaga gaagcgaacg cttattcttg agactgcaaa
    31861 gaggcttttt gctactaaag gggagagtgt ttcgatgaat ggaatagcca aagagattgg
    31921 catttccgtt ggtagtattt atacatattt cccttcgaaa caatcgctta tagagacgat
    31981 tatagaagaa ggctgggacg agtatcgggt ttgggtagaa gacaggatgg tctcaatcat
    32041 ggagtctgga aagcaggacg atggtatagg acaatcgctt tgcgcattgt ccttcttggt
    32101 ggatgaagcg cttccaaagc tcttcagcga tgtcgaactc attatgattt tgctagctgg
    32161 ggcaagctcc aatgccaggc ttgaaaagaa gctacagtac ctaagttcca tgatctccaa
    32221 gttggtcgaa gactcttctg tttccctcga ggttcctctg gacgccaaat tctatcgtat
    32281 tggcataatg gtatttcttt tgggagcgct cgagacgatc aggattactt caaagacaga
    32341 tatcgatatc aatgctgccg atattcgggc tttcctgaga caattaatgg agaatgcgct
    32401 cggtcagcct ttgcccgata gggactgagt gagcggatcc agggctttac ggccattgcg
    32461 gctatgctgc ccaaagggcg aataaacctt gtgcgccatt caacctctga ataatagtct
    32521 tcgagcgatt tacttctgaa atgcagtagc atttcagcct cttccgaatc tgtaaaccaa
    32581 ccacaatgaa agtctcctct ggcaaatatc tcatcgcaca gaaatgaaga atttccaagg
    32641 ccaaacgccc tcataagcct atcgagatag gctcggtaag ttgttcggca ggctggtcct
    32701 ccgcctatat tgaatgttcg accttcaata ttggaaaggc tgcaagccat ggcaaaagcg
    32761 cggccagcat cctcggtatg cacaacctcg aagtgcgttg agggaggcac gtcgaagagc
    32821 ataggatcaa agggaagcca atcgctggat actacatacg ataacctcaa aattacccaa
    32881 ggaaggccac tttctcgaag caggttctcg ctcttgttct ttgtgatgct atagttgtcg
    32941 ttgggatcaa gaggatcggt cgccgagatc catggattgg caagcctatc tccgtatatt
    33001 gagacactgg aggcaaagac aattcgtgga ggcaccgcaa gactcttgca tatttctatg
    33061 atatttctcg ttcccccaac attgatttct tctgcgagta atggtttttg attggataag
    33121 ggaggtatta tggctgcaag atggattatc gcatctattc ctccgactcc agcagaacaa
    33181 atagcatcct gtaggctttt tttgtcagta atatctccat agaagatcgt cagcctatga
    33241 tgcgcatttt tcaacagttc tcgtgttctt ttttgctttt tcgaacgagc agaaggttgc
    33301 tcaaaaataa gcacctcatg gcctttggcc aacagggcac cgactgcact tgttccaaca
    33361 ttcccaaatc caccagtcac taagattcgc atagttctcc tcacatttga tactatcgct
    33421 cattattata atgaatgaac attcattcag caacatagta gttttcaaaa attgagattc
    33481 ttgctcattg acctgctcat aggcctttcg ttacacttgc cacgctatga ttacaatcga
    33541 tgaactgcgc agaaaataca ttgaattctt taaggctaga ggccatgctg agattagcgg
    33601 gaagtcgctc attcccgaaa atgatccaac ggtacttttt acaaccgcag gcatgcatcc
    33661 tctcgtgcca tatctgcttg gcgaaccaca tcctgctggc aagagactga ccgactatca
    33721 gaagtgcatt cgtaccggag acatagatat ggtcggagac tccagtcatc ttactttttt
    33781 cgagatgctt ggcaactggt ctttgggcga ttatttcaag aatgaggcca tacattggtc
    33841 ctatgaattt ttgacaagcc ctgagtatct gggcatagat cctgctaaga tctcggttac
    33901 cgtatttgcc ggcgacgaat cggtgccgag agacgatcgg tcggctgaaa tctggaaaag
    33961 tgtcggcatc cctgaagaga gaatcttttt tcttccacgc gaggataatt ggtggggccc
    34021 ggcaggagag ataggtccat gcggcccgga cacagagata tttatcgaca cagacgcacc
    34081 tgtctgtggt ccggattgca gaccaggctg tcattgtggc aagtactttg aaatctggaa
    34141 caatgttttt atgcaatata ataagaaggc agatggaagc tatgagcttc ttgcccaccc
    34201 ttgtgtcgat acgggcatgg gaattgagcg tactgttgcc atgctgcagg ggaaaaagtc
    34261 ggtctatgaa atcgaggcct ttaccccaat cttgaaggtc attgaatcta ttaccggcaa
    34321 gacttatgga gcctcagtcg gcgatcccga aatcgatcga ggttttcgga taattgcaga
    34381 tcatattcgt gcagcgacat ttattcttgg agaccctaaa gctgttttgc catccaatat
    34441 tggggctgga tacgtgttgc ggcgtttgat ccgtcgtgcc gttcgtcatg gacgaaagct
    34501 cggcatagat ggactcttcc tttccaagcc tgctcaggct gtcatcgaca tctacaaggg
    34561 cccatatcct gaattgcttg agaatgccag ccgaatttac gaagagctcg agcgagaaga
    34621 aaagaaattc ttagagacct tgcaaaaagg agagcatgaa ttcgagaaaa tcctgccaaa
    34681 tctcctaaag aatccagata gaatcatgcc gggcaggctt gccttcaagt tatatgatac
    34741 ctacggtttt cccattgaac ttaccgaaga gctagcagca gaacatgaac ttagggtaga
    34801 taggctgggt tttgaggaag ccttcaagaa gcaccaagag ctttcaaggg tgggaagcga
    34861 acagattttc aagggaggat tggcagacaa ttcggaaatt gtaacgcgtt accacagcgc
    34921 aacccatctt ttgcataaag cattgcgtct ggtgctcgga aaccatgttg ctcagaaggg
    34981 ttcgaacatc accgccgaac gcttacgatt cgatttctcc catcctgcgc ctatgacacc
    35041 agagcaaatc gctgctgtag aacgaattgt aaatgaacaa attgccagag accttcctgt
    35101 gacaatggag atgatgcctc cagaggaagc aaagtctgtc ggggctattg cgctttttgg
    35161 cgagaaatat gagcctattg taaaggttta caccatgggc gatttttcga aagaagtatg
    35221 cggaggtcct catgtttcgc ataccggtga gataggaaaa atcaagatca ttaaagaaca
    35281 gtcttcgagt gcgggagtgc ggcgtatcta tgccgtgctg gaacagactt aggcttattt
    35341 tgctttcatt cttctaagag cctgaaccgc tacgcggtca tcctcaggaa caaggcgcaa
    35401 aaaccatgag ccttcacatt cgaccctttg ccaccagact tcgcgtatag cctcttcaag
    35461 gagcgacttt aggcgccatt ccccttctgt gcaatccata tcctgctgac aaaatatgta
    35521 ctctcctatg ccaagagaga acggcgaagt ccttccgaga tctaaattat ttaaagacgt
    35581 ttgttctata tatttacttt ctgccaaacg atatactgct gatggctcga gcgtgtcaaa
    35641 gacctttggt ccgtcttcag tcatcgtgga aagtccttct tcttcaaatt ccagcagcag
    35701 ttcagactga caacagagaa tatccgatag agcctttgcc gcgataatca gaggctcatc
    35761 gatatggaca tagtcgatat ttcgacatag atgcagtatt ctgagttcaa tctccatgat
    35821 gccattttca aattatatag ccgtgattgt caagggagat gtaaggttca atagtctcaa
    35881 tggtgcgctt gtggacacaa gcagacttag aaggttttga cgatttcctt atgcgaaggc
    35941 tgctcaatgc gaagactgct caataggcgc atgataattt ccagcaaaga tcgcttaaaa
    36001 tcccgctgga aatcttccga aataggtgat aaactgtttc gaaatgtcct tgcttaatca
    36061 gatttctgga ccagccgacc tgcagggcct atcaatggct gatttgcggt gcctctcacg
    36121 ggaaatcagg cttagcataa ttgagaccgt acagcgcaat ggcggtcatc ttgcatcaaa
    36181 tcttggggtt gttgagctta ctattgcgct tcatcgagta tttcaatcgc catacgatgc
    36241 aatagtctgg gatgttgggc accagtgcta ttctcataag attttaacag gtcgagcggc
    36301 tcgatttgta actcttagga gaaatggagg tctatccggg ttcccaaagc gaaaagagag
    36361 caaacacgac attttcgaca ctgggcattc ttccacagct atctcttcgg cacttgggct
    36421 tcttcaggct cgcttgagta tgggtcatgc cggaagagtg attgcagttg taggcgacgg
    36481 agcccttaca gcgggtcttt cgatggaagg actaagcaat gcgggtcaat tgagtctgcc
    36541 tcttatcatt gtcttaaatg acaaccgtat gtccatctcc aactctgtgg gctcattatc
    36601 gagatatctt tcgaagcttt cagcctctgt acaatatcag tccttgcgtt ctcgtgtgga
    36661 tacgatggtt ctgaagatac ctcgtattgg tggagccatt ttttccttta tcaacaggac
    36721 caagcgagcc ataaaggcca tatttttcaa agagaatctc tttgtcgact atggattcga
    36781 atatgttgga cctatcgatg ggcacaatct tgtctcattg ataaatgctt ttgaacatat
    36841 ccgtaatctg aataggccgg tcgttgttca tgtcgttacg aagaaaggga aagggctcga
    36901 aaaggcggaa gaagacccag agagttttca tggccttgtt ccagcttacc ctgatatacc
    36961 agggcccggg acgaccagcc acggagagag ttttacatcc tgttttgcaa atgctctaat
    37021 agcatgtgcc gagaaagaac cgcgtatggt tgctataacc gccgctatgt cttctggaac
    37081 gggggtttca aagctagcgg agcactatcc gcatagggtc tacgatgtgg gcattgcgga
    37141 acagcatgct gtggcattcg ctgctggtct tgcgcaaggt ggcctgcggc ctgtggtggc
    37201 cgtgtattcc acttttatgc agagagcagt agatcaagtg ttccaggaag ttgcgctggc
    37261 taagctgccg gtattgtttg ctctggatcg cgctggagca gttggcgaag atggcgagac
    37321 ccaccaggga atctacgata tcgttctctt taaagccatg ccgaacctgg tgttctttgc
    37381 tcctgctgat ggtgaggagt tgttggcttt cttagatctg gctcttactc tcgacatgcc
    37441 ttgcatgatg cgatatccca aggctttctc accttgtcct gatcttccaa tagcagagct
    37501 cgagattgga agaggagtct ttatgcgtga gcatgaggat gcaagagtgc ttatattggc
    37561 tgttgggccg ctctcatata ttgcgactga tgtatcggat gaactagcaa taaaagggat
    37621 ttttgcagat gtctataatg tccgattcat atcaccaatc gacgaagaat accttgtaaa
    37681 aatctgctct cgatacgaag gagtcttgac aatcgaagat ggagtgctga ccggcggtct
    37741 tggagagtct gtggcgggaa tactaatgcg cagaggaata aagatcaaaa tatcatccct
    37801 ggggtttggt tcggttccac ctcctcaagc gaccagagag gaacttctat caggcgcaag
    37861 cctcgacgca aagggaatac agaaggctct gcatgagcta ttggatttcc tggatatctc
    37921 gaagcagcaa atagcggcag gaattccgac agacttgtct ggaacactat ctacttccag
    37981 cgagatacag catgcgcatt agcgcaaata gaacgcagct aaagcatgat gtgaacaggc
    38041 tacgacttcc gaacgggcgc tcactcgagc ttggcaaagc gccggaacac ttagcctgta
    38101 tcatgggcat cgtcaatatt acccaagact cctttttcga gggtagcaga agacctgatt
    38161 cgatagctgc aatagaaaca gccctcgaca tggtaaagca aggtgcatca atcatagatt
    38221 ttggtgccga atctacgaga ccaggttcta cggaaatcga ttccgaagag gagctatcaa
    38281 gaattcttcc tgtgcttaat ggcttcagag ccttgagcga cgcggtggtc tcggtcgata
    38341 cgcgccaccc cgaagttgca gaagcagccc ttgctgccgg agcggatata atcaatgata
    38401 tcgaagcatt gcaaagaccg ggtatgatcg agaccgtgtc aaaggccaag gcagcagtca
    38461 tccttatgca tatgcaagga actccgcaga gcatgcagaa agatccttac tataaggatt
    38521 gcctccaaga agtacgctct ttcctcgaag acagaagtca tcgtgcaatc gaagctggca
    38581 tatccccaga caggataatc ctcgatccgg gcattggatt tggcaaactt aaggaacaca
    38641 atatttcact tttgaagggc attgagcata tcgcttcgct tggttaccct gttttgattg
    38701 gtctttctcg caaaaagctc attggagatc tgaccggcag ggatgttgct agcaggctcg
    38761 cgggcagttt aggagccgca ttggctgcct ggctaaatgg cgctcagatt cttcgcgttc
    38821 acgatgtggc agagactgtg gacgcgcttc gagcctttca tgaggtgata caatgaatgc
    38881 accgactttg atcgagttct ttactatata tgtgcggcct gtgctcgatg taggcattct
    38941 cgcttttctg atttacaaga cctatcagct tcttgtaatg accgatgcgg tctatcttat
    39001 tcgcggtttg ctaattcttc tcgctatata tggcatatct tttttcctaa agctctctac
    39061 agtcacatgg atcatgaaca tactggcgcc aggattggtg ataggtcttg ccattatcct
    39121 tcagcctgag ctcaggcgga tattcattca aattggccgc caatcgattt ttggaaaaaa
    39181 atctatggct cgcagctcct ggattgaagc tgcggtatct gcttctgttt atcttgccga
    39241 aaaaaggcgc ggggttatca tagtcatggc ccggaaagtt gggctagcct cttacattga
    39301 gaaaggtatt ccccttgacg caataatctc aaccagcttg ctcatatccg tgttcgaaca
    39361 cgacaaccca ttgcatgatg gcgccgcaat catctcacag ggcaggcttg cggcggccgg
    39421 ttgttttctt cctctttccg atcagcaaga aatccgcaag acttttggct caaggcacag
    39481 ggctgcgctt ggcattgccg aagaatccga tgcggttgtg ctcgttgtct ccgaagagac
    39541 cggagccctc tctctcgctt atgattcaaa gctctattat gatctttcct cagaagaaat
    39601 tctggaacgt cttcatattc tcttctcaga gcagaatctg agaagagagg gaaacgaggt
    39661 tgccaatgag ccttgattcc cggatttcca aattgctgga gaattggccg gctaaggtca
    39721 tttctcttgt tatggcgatt tttataatgt ttctttacaa tctcacccgt ctcgaacagc
    39781 gtgttataat cgtgccactg aacatctctg aagggcacag ctatgtgcct gccacggaat
    39841 atccaaagac tgtccgtgtg ataatccgcg gtgaccgtga tcaaatctat agagtccggg
    39901 aatcggatat agtcgcgagt cttgatctgg cacgttatgg ctccgaaggc gtattcaggg
    39961 ttccggtcaa actcgagagg cgaggggatg ctctgaatat cgatccctta gagttaagag
    40021 cggatccctc agaaataccc atcagttttg agcgcctggc tgcaaagcgc gtggctatta
    40081 ctcctacctt taaaggtttt ctggaacaag gctatgtatt gatctcctat gaaatcttgc
    40141 catcagagat atctatagaa gggccaacga ctttggttag cagtatcaag gatatttcca
    40201 cagatataat agagcttagc ggaaagacca gcgatttttc tcttaatgtg cctcttgtta
    40261 agccttctga actaataagg cttgtcgaca caagcacggt aaagttctct gcgaagattc
    40321 agatgcagga aagacggctt acattaaaga atgtgcctat attcattaac aatctcgatc
    40381 catcgcttat aatctctgat ccgctcccat ctggaaatat aaccctcgta ccacatcctg
    40441 gacaggatga gactttggca aacaatgcta gccttcaagc cgatttcaag ggcatagtta
    40501 agcctggtct atactctata ccgcttactg taagcgctcc ggagggcttc gaagtcgaag
    40561 catacgagcc tctggcgctc aatgttcgct tgcatggcgc gccagttctt cctgcctttc
    40621 attgaaatta caaggagtcg atccatgatt tccggaattg gtatcgatat tgtccatgtc
    40681 gatcgcattc gccgatggat gaatgaacca gggatactaa agcgattctt ccatccagcc
    40741 gagatcgaga ccgcgagcct tcgcaaaaag ggcgctgccc tttccctggc tgctcgcttt
    40801 gccgcaaaag aagcctttgg caaagcgctc ggttctggcc ttgccaactt cgcactgaag
    40861 gaggtcgcgg ttgtaaacga tgatcttggc cggcccataa tgcgcctgga agggaacgcg
    40921 cgccgcgagt tcgagcggca tggtggagga aagatccata tctctatgac tcacgaagga
    40981 gacaatgcag ttgccatagt aatcttagaa aaaggaagct agcagatggc agagcatatt
    41041 cacgcccagg aaaatccttt ttgcgcgcca aaaccatcat tacgaaccat cagactcgta
    41101 attgcttacg atggcagcga tttttccggt tggcagaaac agaaaaacgg acgatccgtt
    41161 caagaggaac tcgaaaaagc gcttttgata atgcataaac acgaagtgcg agtcatagga
    41221 tcagcacgga ctgacgctgg cgttcatgct atgggacaag ttgcaggttt tcatacagat
    41281 atcgcttcga ttccagccga tcgtttcgta ccggccttga atagactctt gccgcgagat
    41341 attcgcgtac tctcttctga agaagtaaag cctgactttc atgccaggtt cgacgcttct
    41401 ttgagacgat atcgctattt tatgagctgt ggaatggctc ctgatccatt tgtgcttcgc
    41461 tacactcatt tttcccctca ctatcctagt ctttctattt taaattctat ggcttttgtg
    41521 gtgctggggg agcacgattt ctcgaccttt gcctctgcac aggataagag cgaatcaagg
    41581 tttcgcttca taagcgagtc cgtgtttttc ctggagagag gcttgttcgt ttaccagata
    41641 gcagggaacg cttttctatg gcgtcaggtt cgctctctgg tgggaacctt tctagaactc
    41701 gaaaagaagg caaaaaaccc ttgcgaagca gaagtcatga tgcgagaagt tcttgaatcc
    41761 aaagatcgaa gcaaggcagg gcccacggcc ccggcctgtg ggctttttct ctggaatatc
    41821 gaatatggag agcgagttca tggccatccc gcgaaagcag tgtaatagaa aagaagacca
    41881 aaagacagaa ttggtatctc cggtttggga aggaacagcc aatttatctg tggccataag
    41941 ggctgataga tttctctcag agataattaa gctcacgacg cgttctcagc tcaaggctcg
    42001 caacgctcgc cttttctgca acggagtaga agtcaagctc tctcataagc tgaaaaacgg
    42061 agaccacttg cgtcttgaat ggaacgaagc catttcagaa caactggagc ctgaacccat
    42121 accacttgag attatttacc gcgatgcaga tgtctatgta atagataagc ctcagggaat
    42181 ggttactcat cctgcggctg gcaattggca tggtaccctt gcaaatgctg tactatggtt
    42241 ggagaacaat gtccgccaag ataatcaaat taaggattct tcatcgatta cttctcctac
    42301 ccgtgctggt attgtgcatc gtctcgataa ggacacttct ggagtaatca ttgtagcccg
    42361 atcagcttct gtacaggaat tcttatcgag tcagtttaga gaacaccagg ctagaaaaga
    42421 atactttgca atagtgaggg gaatgcctcc tgaaccgaaa ggcagaattg atacatggat
    42481 agcgcgaagt cctagagatc gcaaaaagtt cgcagtgagc gagcctggac gcggcaagca
    42541 tgctctcact ctatatagag tctgcaaagt atggcaagtg aattgtaata ttggttatag
    42601 atcggtaggt gttgcttcga aagcgacaaa gccttctaag tattcgctgc ttgctctata
    42661 cccaagaaca gggcgaactc accagctaag ggtacactgc gccgatatgg gttgtcccat
    42721 tctaggagat ccaatctatg gtaagaagga tgaagccttt cccgaagcta cccttatgct
    42781 ccatgctcat cggctcaaaa ttcgtctgcc ttctcgatca gaaccc
//
LOCUS       DAOOWY010000006        38518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1097_length_38518_cov_10.419962, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000006 DAOOWY010000000
VERSION     DAOOWY010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 38518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38518
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1097_length_38518_cov_10.419962"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..1152)
                     /locus_tag="PK105_01050"
     CDS             complement(391..1152)
                     /locus_tag="PK105_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU60320.1"
                     /translation="MLINGIVVLIIVSMLLLLEYILALRCLFADRYERFHLCAKYEQI
                     GAKIILGLFRVFRGFRIRVHNPERLEIPLRCLVISNHQSLLDILVLIAFLGIERKPRF
                     VAKKELQYGIPFVSFTLRKGGHCLIKRKGAPLDAMREVSKMAKICAKEGACPVIFPEG
                     TRSRNGHVGEFHTAGVRKLLESGGRLSVAAIALEGGWRVANAMDFLKLFGKTPYIVEI
                     VKIYEAPRNKYDIENMLIDARRLIEERILELRRLQ"
     gene            1298..1567
                     /gene="rpsT"
                     /locus_tag="PK105_01055"
     CDS             1298..1567
                     /gene="rpsT"
                     /locus_tag="PK105_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOU60321.1"
                     /translation="MSTTNLSAEKRQRQNEKRRLANKSTKTSIKSAAKKVVMASEKKD
                     TEAAKQALSEMIKRIDSAARKGIIKKNTAARKKSRMQRLVNKIDQ"
     gene            1609..1953
                     /locus_tag="PK105_01060"
     CDS             1609..1953
                     /locus_tag="PK105_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU60322.1"
                     /translation="MAEKLTKAELIDALYDSLSSTSRTTRKEIHELIDGLFAEIKGAI
                     LDGKIVELRGFGTFEVKLRKGRSKARNPKTGEIVSVTDHGVATFRPGRELKRAAWEMD
                     IERIPKSFRKRV"
     gene            2014..2400
                     /locus_tag="PK105_01065"
     CDS             2014..2400
                     /locus_tag="PK105_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU60323.1"
                     /translation="MKKSNLEPSEPYATHIGPALKLNGILRSNQPLDLHCAFHGSIEG
                     TFIKISKNAIIEADLLKAHQVVIAGQFKGSMSVSEEIIILPNANVSSDIKVACLHIIE
                     GSQFEGKIEMAGFYPDHNSPIVDRGH"
     gene            2590..4338
                     /gene="rho"
                     /locus_tag="PK105_01070"
     CDS             2590..4338
                     /gene="rho"
                     /locus_tag="PK105_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOU60324.1"
                     /translation="MAIHNRKKIDSSQTTSSNNDENNSAEPREEIAMNQSQHEEQPVL
                     KNESLFEEQTEDLVQDIQRSLNNVHVAAPVKNQQQKTRKPVAKIRLKKKPTQKAGLLE
                     ETQVPESTTTSEVIEEQPNGFVVAAMPLDEHQNQRLSELPKPEPENRTEQRVESRARL
                     LINDLSKMGIIELRELGAKYGINHDLLITLKKQELIFNILKGHTEHGGIIYAYGSLEI
                     LPDGYGFLRSPQNSYLPGTDDIYISPSQIRLFNLKTGDTVYGQIRSPKEGERFFAMLR
                     IEQINFDDPSVAQNRIPFENLTPLYPTEKLDLETSTEEISTRIINLFCPIGKGQRSLI
                     VSPPRTGKTILLQKIANAITANHPEVYLIVLLIDERPEEVTDMERTVKAEVISSTFDE
                     QATRHVQVAEMVLEKAKRLVEHKRDVVILLDSITRLARAYNQTVPTSGKILSGGVDSN
                     ALHKPKRFFGAARNIEQGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEINLDRRLS
                     DRRLYPAINIKKSGTRKEELLISEEELQKIWVLRKVINPMDDAEIIELLIDRMTKTKN
                     NDAFLKSMNTPYSSME"
     gene            4441..4656
                     /gene="rpmE"
                     /locus_tag="PK105_01075"
     CDS             4441..4656
                     /gene="rpmE"
                     /locus_tag="PK105_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOU60325.1"
                     /translation="MKKGIHPKYDITTITCACGNVIETRSTAKNIQVEICSACHPFFT
                     GKQKLVDTAGRIERFNKKYGIKTNENK"
     gene            complement(4737..5342)
                     /gene="rdgB"
                     /locus_tag="PK105_01080"
     CDS             complement(4737..5342)
                     /gene="rdgB"
                     /locus_tag="PK105_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOU60326.1"
                     /translation="MRFVVATNNLHKIVELKPLFPCHELLMPSDLGINEFCPEENGKT
                     FFENAYIKARSLYELLGIPSMADDSGLCVEALGGKPGIGSARYGSNGKELLAASEKNS
                     LLLSELAGVSNRNCAFVCCLVFYYGKQRFMCVQETLEGEIAGSPSGKGGFGYDPIVYL
                     PQYGKTVAELSADEKNRISHRGKAAQRMATMLATLSLGCRD"
     gene            complement(5352..6194)
                     /locus_tag="PK105_01085"
     CDS             complement(5352..6194)
                     /locus_tag="PK105_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60327.1"
                     /translation="MDSQTYLDLVRKMYEHPKRYGFFTKDEISDALNNYRNRIELILE
                     RAENESRTKDAYLLSSMRYVAKSVHRQNYNLSLCETAYIYSNFTEELAIESPVEPWTY
                     YETINDATTEKGILNLPPKVILDKLSPAHKRLLFLTMKCAWEIDEELLSRCSLSLGIP
                     REYLFNLIELTKRRTERGWNKAEQMNIKLHALWIRLRVLEFRLETCLLNEEKEYILCS
                     LKRCRERYARLLEKRCHRRSAVSNESISQILGVPKGSIDSGLFYLRKHVDSGRSLEDY
                     RKLG"
     gene            complement(6628..7614)
                     /locus_tag="PK105_01090"
     CDS             complement(6628..7614)
                     /locus_tag="PK105_01090"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOU60328.1"
                     /translation="MRQVPKILIDFINHHDCFYILGHREPDGDCIGSQLALASMLQSM
                     GKRVHVLSSGPFNRIEILRFEARFKSEVPSERDFERTAAIVLDCSSMERIGSVAERMP
                     NIPASFIDHHSTSSAPGPYDWLDESASAVSAMILLLMEAMGHSPTREEAELLFFGLCT
                     DTGFFRHLDEHGSESLKIAARLVEAGVSPKRVYMAINGGRTLASRRMLGELLLRIQPY
                     YEGRLLVSWVTIEDQQRYGMSSRDSDLLYQLMMGIVDCEVCFVVKQETEEMCTVGLRS
                     KNSINVARIAEQYGGGGHRLAAGLSINGKVDEVVHTLVRVFGDVFRDEEIEK"
     gene            complement(7645..8586)
                     /gene="argF"
                     /locus_tag="PK105_01095"
     CDS             complement(7645..8586)
                     /gene="argF"
                     /locus_tag="PK105_01095"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOU60329.1"
                     /translation="MNPSILKGRSLLTWIDYTPEEIRYFLSLSKKVKEEAKKGMHKQR
                     FRGKTMALLFEKRSTRTRCAFETAFGEEGGHPVFLSNQDIQLGAKESIEDTARVLGRM
                     FDAIEFRGYKQEYVEILAKYSGVPVYNGLTDLFHPTQALADVLTIEENLGNCAGKRLC
                     FIGDGRNNVARSLMVISAKLGMHFTIVSPRELWPDNELKNLCEGFAKTSGSTIIITDE
                     IESGVNGADAIYTDVWVSMGEEALKAERIRMLSPYQVNAELMAKTGNKACIFLHCLPA
                     VKGEEVTFDVIEGPQSKVFEEAENRKHTIKAIILATL"
     gene            8674..9384
                     /gene="nth"
                     /locus_tag="PK105_01100"
     CDS             8674..9384
                     /gene="nth"
                     /locus_tag="PK105_01100"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOU60330.1"
                     /translation="MSNELEISLKEINESIPLPQLLEELYIRLVPIWPDTRPLLHYNN
                     CFELLISVILSAQTTDDQVNIVTKELFHRFPNSESLAAADIHEVENIIYPLGFYHVKA
                     KHIIETAKVLQERFNGQIPSNFEEMIELPGVGRKSANLLIAACYEKPGIIVDTHVLRV
                     LFRLGVSAKKEATLIESIIRANLPPEKHTEFSYAVNRHGKFTCTARKPACTEQKKHCP
                     IDDICPKIGLSALLKQRR"
     gene            9414..11084
                     /locus_tag="PK105_01105"
     CDS             9414..11084
                     /locus_tag="PK105_01105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOU60331.1"
                     /translation="MFKPDLVSESEDPIGILGRDRFHIPHLTPLQRFVIANILDETKG
                     SAMLTRNLSSDPIDDDAVSNLNKENEFQERQETYRQLILFPTGFGKSVCFQLPSLLLD
                     GLTIVVYPLLALMNDQKRRLDEANISCALFRGGLSQEEWNEEEKLVSSGKANMVVANP
                     EILSAPKLRRFLNRFKIAHFVVDEAHCISEWGESFRPSYLALGETVEAISPGILSAFT
                     ATAGPHIVHSIEKRLFMDKDYRLVDSEADRSNILYAVVHTLSVTRTLRQTLLCCKKPA
                     IIFDRSRPGTRLKAELLKASGFSQARFYHAGLDRAERCNIEKWFQDSDDAILVSTNAY
                     GMGMDKKNIRTVVHSSLPESAEAYIQEAGRGGRDGKNALAVLIDDLAVRYYRENLKDK
                     NIQDIQSIRKACFLPYPTLSSCRREFLLHLLGEEKTPECGRCDNCIEGSHTQRTQFNS
                     DASDILPEWKFLSRAEGFIEAISFCDSHHGVFTSQELVDALGPHGKGKGEYAGCLYGW
                     LKIEREELINALFLLNILYAPKKGTRKNRILLSQKGKALSRLARNSLV"
     gene            11167..12774
                     /locus_tag="PK105_01110"
     CDS             11167..12774
                     /locus_tag="PK105_01110"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOU60332.1"
                     /translation="MVVFKNLDTCQAFEALKKIPAVVLHKSLDQKRISDWWIPHAAGF
                     CYCYAFAPVDDSVIDWLEALTREQQLIEKYRILTEGAIMNTGEKRMVLHHLVRGRLGK
                     PVIWNGQDMEVFYREEKHRFYDFAHKIREGEILGTTGKAFTQIVQIGIGGSDLGPRAA
                     SLALSRWAVAAGRARLRPWFISNVDPDDADYVLANIDLSESLFILVSKSGTTQETLAN
                     ETIVRDRLQKAGLNPDKHMVAVTSTSSPLAHNPHYLDSFYIDDFIGGRYSTSSAVGGV
                     IISIAYGPETFEEFLEGANEADRLAFEPSLRKNPVLMDALIGIYERNILGWPATAVLP
                     YSESLSRFPAHLQQLDMESNGKSVNREGHPISYSTGPLVFGEPGTNGQHSFYQLLHQG
                     SDIIPLQFIGFLRNQLSPDMISEGSSSRQKLLANLIAQMLAFAIGKKDENPSKTFSGN
                     RPSTLLLARELGPRQLGALYSHFENKVMFQGFVWNLNSFDQEGVQLGKILAKKALSAI
                     TKGESTDDELLDAFIASALRAVENNGD"
     gene            12819..14048
                     /gene="murG"
                     /locus_tag="PK105_01115"
     CDS             12819..14048
                     /gene="murG"
                     /locus_tag="PK105_01115"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOU60333.1"
                     /translation="MQSIYETELQEKDELENDCIAFTGGGTGGHIYPGLAVIEELRRL
                     GFKGRIAWIGSEKELDRSIVTGQGIEYHAIPSGKFRRELSLRNISDLGRVASAYFRAR
                     ALLKDIGPSILFSKGGYVSVPPCWAASSLGIPVITHESDASPGLATRLNSKKASLILT
                     SWEVTAQYFPESLRTKIIRAGNPVRPDILNGDRQRGLSMLEFVAEKPVLFVLGGSQGA
                     QEVNDIVLSSLNLLVPLVQIVHQTGRANFDEVKARVPDDPAVRRAYRPFPYIGSEIAD
                     IYAASTMIAGRAGAGTIWEAASLGKPMILIPLSGSGTRGDQVENAALAERSGIATVLL
                     KPNISSNLFVDAVKKYLNIKNYAMAEEACRNLVHIVKTPHIPEITYGPSQNPILSAEY
                     IAQLLLTKMAEWAKESA"
     gene            14045..14578
                     /locus_tag="PK105_01120"
     CDS             14045..14578
                     /locus_tag="PK105_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOU60334.1"
                     /translation="MSTIDWVFSGIVIILAARCFVRGFIHEILSVASIALGILAGLLL
                     SNTVILQILPKINADKLPYQAQYIIAFILCFIAGFLLMKIIERMLRQGLEASNLDILD
                     RILGLILGVAEGFIVVGLFIVILEIQPIVDAKAILSNSLYVKLLGPIVEPAIGGTLSP
                     MLKDMDMPILKQKPKGK"
     gene            14575..15879
                     /locus_tag="PK105_01125"
     CDS             14575..15879
                     /locus_tag="PK105_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60335.1"
                     /translation="MIMFENLLFQEHALEQLVSLAKEKKVPPALLFVGPAGSGKLTAA
                     LECARVLSCEKEAEWNCECTQCRCHRNLMHPDLLLFGPREFAQETIITGEYLIGKPGL
                     TSYYAFVRSIRKLLKRFDSVLWQGEEARLSKAIPSIETCEELIQEISELVLKKKNHAL
                     TVAVENAINAAAGLESLVPDGIPVFMIRNMISWAVLAPSAKTKMIIIQNADAANESAR
                     NAMLKILEEPPETVRFILTSSRRAAVIATILSRSRLISFDSRTGDQAREIISRLFHVD
                     EDLQNINSISEFFQNKSPYPPKVATRDAALFIGALLGKTMKENPGLSGKYAIELASMA
                     QCENVSALSILRQVSDKTNAFGAKNSRFANSFLVFMRAVSQHLAHIAADPSSSPSLIM
                     YVDRLFSKLREMNMQYRTLNRSPELMLEAFADILGEQYESRI"
     gene            15863..17056
                     /locus_tag="PK105_01130"
     CDS             15863..17056
                     /locus_tag="PK105_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU60336.1"
                     /translation="MKAAYENTLKRIDRIPPEGLKQIVRNLLAENSIYESALNSTLDG
                     IIVCDLAHKPLFINRSAEKITRIVSWAQEVVLWEQLDDDNLADFIQEALSSEKNILNH
                     ELSISRPGGTRLLSISISALVSEGKIAGNLIHIEDITEKRRREAQLRRAESLAALTTL
                     AAGVAHEIKNPLASIRIQLGIIRRILEKNCAKKTEPIFHNISLVEQEIDRLNSIVVDF
                     LFAVRPMDITLIRNRVEDVIKEVVEIMGNEASVHHINIVCEFEDNLPEVMIDPKHLKQ
                     ALLNIVKNAIAAMPNGGTLTISAVTRHDELQISISDTGIGIPEELLAKIFEPYFTTKE
                     SGTGLGLTITFKIIKEHNGEITVESKPSKGSTFTIHLPIPQPEQKSLPPWEEGLQIEG
                     SEREE"
     gene            17057..18457
                     /locus_tag="PK105_01135"
     CDS             17057..18457
                     /locus_tag="PK105_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU60337.1"
                     /translation="MRATLLVVDDEINIREGLAQLFSLEGYDVKTAKDGLEGKMIVDK
                     EDIDVVITDYKMGRMSGLDLLQYIVSTHPGIPVIILTAHGSVDKAVEAMREGAFDFIA
                     KPPNTDHLINLVRRALETRELYRTNIELKAEVESQRARSYIIGKAPSIKRIFDLIRKV
                     APTKASVLITGESGVGKELVADAIHNLSPRRDKPFIKVHCAALAESLLESELFGHEKG
                     AFTGATGRKRGRFEMADGGTLFLDEIGEINQNVQIKILRVLQERKFERVGGEETIEVD
                     VRLIAATNRDLKREITEGRFREDLYYRLNVVNIHVPPLRERREDIPLLAMAFLREFAD
                     ENGKQIEGFDPRVRQALYAYDWPGNVRELRNCIESAVVMASGKLITLDDLPPGPRSSG
                     EARTISIPAFSSLEEAERIIISETLALVGGNKSKAAEILKIGRKTLYQKIAQYGIGKQ
                     EANSSVSSNPREETEN"
     gene            complement(18489..19292)
                     /gene="whiG"
                     /locus_tag="PK105_01140"
     CDS             complement(18489..19292)
                     /gene="whiG"
                     /locus_tag="PK105_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HOU60338.1"
                     /translation="MEKGKGRIMDYATLEEDRLWELYRRTEDIHIRNEIIMRYAPLVK
                     YVAGKVSVTLPSMVEYRDLVGYGTFGLFDAIEKFDPNKNVKFKTYAVTRIRGAIYDHL
                     RELDWVPRSIRRKTKQIERAIAELESQLGRPATDEEIAKEMGIDCKELLKIMSKVASS
                     SILSLHEIWPINSEAEGYTIEDSLESTRLENPDLVLEREEIRRVIFEALQELPEKEKK
                     VMVLYYYENLTLREIGKILEVTESRVSQLHTKAILRLRAKLSNKKRGII"
     gene            complement(19292..20659)
                     /gene="hslU"
                     /locus_tag="PK105_01145"
     CDS             complement(19292..20659)
                     /gene="hslU"
                     /locus_tag="PK105_01145"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOU60339.1"
                     /translation="MIIEKDALTPHKIVEELDRYIVGQEKAKKAVAIALRNRIRRQCL
                     PPEIREEIAPKNILMIGPTGVGKTEIARRLAKLCNAPFLKIEATKYTEVGYVGRDVES
                     MVRDLMAAAVSMVKEEMDSQVATEAKRRAEERLLDILLPGLAKSDEPIGQSQATLIIP
                     NSGMETKEKFRTLLKEGKLNSRQIEITVPAQGPQIELFAGTQFEEMNIALGGLQGLFG
                     GSKKKKTVSVAEALPILEAEEKEHLVDQEKVVQEARRRTQELGIIFIDEMDKIANRDA
                     RGSSGIDVSREGVQRDLLPIVEGTTVNTKWGPIDTTHILFIGAGAFTVAKPQDLIPEL
                     QGRFPIRVELDALTGDDFVRILTEPKNALVTQYIQLLSTEDVQLEFNGDAIARIAEIA
                     AAANRQSENIGARRLQTVMERLLEDISFDADKHTSTKVVIDRAYVDERFKDYVQQQDL
                     SKYIL"
     gene            complement(20656..21246)
                     /gene="hslV"
                     /locus_tag="PK105_01150"
     CDS             complement(20656..21246)
                     /gene="hslV"
                     /locus_tag="PK105_01150"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOU60340.1"
                     /translation="MELHATTILAVRKDGHVAMAGDGQVTMNNTVLKSNARKIRTIYN
                     GKVLCGFAGATADAFTLFEHFEAKVQEYGGDLTRASVELAKDWRTDRVLRRLEAMLLV
                     ADQSKMFLLSGTGDVVEPSEDAIAIGSGGSYAYAAAIAYLDASRRMKEAARSTPGEDA
                     GMLPDSFSAREIAERSLKIASSICIYTNDQIIVEEI"
     gene            complement(21264..22160)
                     /locus_tag="PK105_01155"
     CDS             complement(21264..22160)
                     /locus_tag="PK105_01155"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOU60341.1"
                     /translation="MDARLLDYLDYLASVRGLSIRTVEAYRRDLAKYEIFLEGKDLDE
                     ADASDIRTFAGTLVMEKRAASSINRALSAVRGFYRYWMKFHKKGKDPAREVENVTVGR
                     TLPSFLFEEEINSLLDGLGGDTFLDVRDRALLEVMYSTGSRVSELCGMRLSRLNTNNC
                     TIRVKGKGSKERLVFLCDAANTALGQYLPYRAAILRKYGIEEHDRIFVNSRGRPLSSR
                     GVEKIVENRRLKAGIRKHLTPHTFRHSFATHLVAAGADMRVVQEMLGHSNISTTQVYA
                     HVDMERLRRVYEQAHPHGSKTK"
     gene            complement(22164..24326)
                     /gene="topA"
                     /locus_tag="PK105_01160"
     CDS             complement(22164..24326)
                     /gene="topA"
                     /locus_tag="PK105_01160"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOU60342.1"
                     /translation="MTAKKSKEKKTPEAAKAAETHKLKTLVIVESPAKARTIEKYLGS
                     RYKVLASNGQVIDLPKSRIAVDVAHNFEPEYITVRGKAEKLKELTEEAKRSSAVILAA
                     DPDREGEAIAYQIGKYLKDRAKDTPISRVTFNEITKQAVQDAMANPRDIDMNLVEAQK
                     ARRIIDRLVGYTLSPLLWKKVKSGLSAGRVQSVALKLICDREKEVESFIPEEYWTIEA
                     HLKAHQHIVRAELALLNGEKPSIKSEVEANNILALFLGKPALVSDIQSIDRTVKPKPP
                     FTTSKLQQAASNRLGFTSTKTMKIAQQLYEGVDMGHQRTGLITYMRTDSTRISEAALH
                     EIYEWLTKYFPNQTPAAPIRYSVSNAAQDAHEAIRPTRVDITPDEAGRYLKGDQLKLY
                     TLIWEQFVASQMKPAIIRTLTADILIGDGIFRTSASSFLEEGFYKVIRLAASKEERAS
                     HHLPFEKGETLQVERIESEQHFTQGPSRYTDASIVRTLEELGIGRPSTYAPTIETLIE
                     RYYVHRDKRQLVPTQLGRIIDDLLSQNFPEVINTSFTAEMETMLDKVEEQKIDWVSEL
                     KKFYFPLVDKVENALNVLEDMHGALDEKTDEKCPICGRPLVKKLGRFGYFISCSGFPE
                     CTFTKSVPLATCPKCGGDIVPRVSNKGRRKKFYGCSNYPECNFKTLYKPSNSLCPKCG
                     WFLVEKYDKKTGHRKLCINPDCDHLHSSQQSGENNGES"
     gene            complement(24390..25418)
                     /gene="dprA"
                     /locus_tag="PK105_01165"
     CDS             complement(24390..25418)
                     /gene="dprA"
                     /locus_tag="PK105_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOU60343.1"
                     /translation="MKAEKEIRCAYPHGGFSQMTLGKQHKGIGKLIQLELDRISAIQP
                     SMEKTPLALRLAFQRLHFLSWQERILLLENIHDMEDFLALGLAEIESFLYRSMHARPL
                     DMGKIWEQAERDINILDRLGARFVCVVDKDYPPLLREIHRAPFGLFVRGETKALNQAS
                     VTMVGTRSPTSRGLYTAQLLAKETADEGIVIVSGIARGIDAASHRGAIQSGAAATIAV
                     LPCGPERVYPSSNAALAAKILDCGGCLVTEYPPGVSLDRYRFPERNRILAGFSKLTLV
                     VEAPEKSGALITAEIALSEGRDVAVAASCLGSSRNSGADALKEDGALAIKAGAELVPM
                     IRCEYNGI"
     gene            complement(25437..26333)
                     /locus_tag="PK105_01170"
     CDS             complement(25437..26333)
                     /locus_tag="PK105_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HOU60344.1"
                     /translation="MSILLRRFQAYLLATRRRSVLTADAYLREIEMLENFLFQYGKTP
                     LDAGEEDLLAYLVSRSQSGLQRTTMARIIASLHSFYRFCLNEKLRADDPSMQIRTPKQ
                     DRNLPDVLDPDSIEHVLECIDITSPNGLRDRALFELIYSCGLRISEAANLKFQQLYLE
                     ERLLRVIGKRKKERLVPFGDEAKYWLSRYLAEARPLLEKRERTDFVFLNQEGKGISRK
                     GIWKRFSKIREKSGVNAKVHTFRHSFATHLLAGGADLRTVQELLGHSDIATTQIYTHI
                     DEESLMMYHKEYFPRKSEKNKA"
     gene            complement(26330..27577)
                     /gene="ftsZ"
                     /locus_tag="PK105_01175"
     CDS             complement(26330..27577)
                     /gene="ftsZ"
                     /locus_tag="PK105_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOU60345.1"
                     /translation="MAIELVDDNGIGSPTVIKVIGTGGGGSNAVNRMIMAGLKNVQFI
                     AVNTDLQDLGRAKAEIRLGIGSKITKGLGAGGKPEIGEKAAIEDRDKIEQALRGADMV
                     FVTAGLGGGTGTGSAPIIAQTARDLGALTVAVVTKPFAFEGKIINRIAEEGFSKLKQA
                     ADTVIVIPNQNLLRVVDKKTPLKEAFRIADEVLRQGVQGISDLITLAGDINIDFADVR
                     TVMEGQGDAIMGIGVGSGDNRAQEASEKAINNPLLDDACIEGARNILVNVTCGEDFSL
                     IEYQEVIDLITSKAAEDAHIIVGVVTDSSIKDEVRVTVIATGFGALHQKLGEKTVTKN
                     TIAMPLGSIRPSEFLSPGEWPLHEEKPAQKRDQASENQDKSSFKDNFGRISFDPESDN
                     DEFDIPTALRNKRLMPEDFGGRR"
     gene            complement(27599..28810)
                     /gene="ftsA"
                     /locus_tag="PK105_01180"
     CDS             complement(27599..28810)
                     /gene="ftsA"
                     /locus_tag="PK105_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOU60346.1"
                     /translation="MDIGTTVTKAIVGGFNEMGQFEIFGIGAAPSSGLRRGVVLNIEG
                     TLNSINAAIDAAELMSGIDIRQVSLALSGANIESFNTNGQIAVTGRGREITAEDIARV
                     HEAARAVSISMDREILHVVPRSYTVDDQKGIRNPLHMTGVRLECSVHIITGSVSTVQN
                     MLKCVTRAGYKVEQSYLGILGCARTVLTEDERELGCLLLDIGGGTTDLMVFSESDPVY
                     TSAIPLGGSQITGDISIMLSIPIDSAERLKKESGAAWTEAIDPDEAVIVPGFGAREPV
                     ELERRKLVSIVQPRVEEIFEMVNERLVKNGSKELIKAGVVLTGGSALLPHIDECARSV
                     FGVPVRIGAPTALPGLDVGFRSPAFSAAVGIVLLEAERIGTPEPSTRRKARKGKEGGF
                     SPIKWIKDRFF"
     gene            complement(28827..29984)
                     /locus_tag="PK105_01185"
     CDS             complement(28827..29984)
                     /locus_tag="PK105_01185"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOU60347.1"
                     /translation="MTDVRLQEQGNETKALRKKGTHSLLSKSDNYSIDIIQATTRCET
                     ALPGSSTAKSSGTRINAGEKIQISQRINTSSEQAHRATEEKAQSLSATKISVNPFIRR
                     APQDSVALSVQAEKLSESVKPAKTSMASKRHRSLHILIAITLLCVLSAGAAISLPRLT
                     KIDAVRVSGLETISENSIIESLGQIADENILSIDIEEIASSIMKNPRIAGAKVYRVLP
                     STLAIDIRERLAVASITINTEQGSKLILVDGEGIAFASLDADDSNNPELPVVSGIRFE
                     QFSLGQQLPEAILPLFANLAVIKQDSPELLRAFSEIHVERISANNIEIVLYPMHVKTA
                     VRLPLRFSAEALCNALVVLDILHARGLSDQPSEIDFHSGTVIYQAKEGVSG"
     gene            complement(29977..31146)
                     /locus_tag="PK105_01190"
     CDS             complement(29977..31146)
                     /locus_tag="PK105_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOU60348.1"
                     /translation="MKEESKSKSLRIDSHRIDWQMVCAIIIVSLIGIMTLWSGSIGFA
                     IRNGGPNSVIIKQAILYAASLICMAVAAVIPMKLLRAVLPVAVVLCVIGLSLPIFSPL
                     GMTINRARRWIRIGSFSLQPSEIWKPVMVLYLASFLEKRKERLVESGLVALPAIVLVI
                     ISAVLIFLQQDFSTSILVLAIAFAMLYLAGTPFLFMSNLALFLILYGALMIFSSQYRV
                     QRLVGFLMPEQHTQTVNYQMYSALRAIRSGGVWGKGVGLGTLKISSIPEVQSDFIFAA
                     FVEESGFAGVLVVMGLWSFILIRAGRILIGSDYFSRLAGFGMITLLSLQVLLNLAVVT
                     GVMPTTGLALPFFSAGGSALLMSAISCGILINLSRSGQTETGMRAALRKEGAAYD"
     gene            complement(31159..32241)
                     /gene="mraY"
                     /locus_tag="PK105_01195"
     CDS             complement(31159..32241)
                     /gene="mraY"
                     /locus_tag="PK105_01195"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU60349.1"
                     /translation="MLREFLLPLVKYFTPFNIFRYLTFRSAYAAVTALLISYVLGPWL
                     IDRLRVLKFGQSVRLDGPQSHLAKTGTPTMGGILIILSVLISVVLWIDISSVYCWIAL
                     SALIGFGLIGAADDILKIKKHSSDGLSVVQKLILQFIVSTIIVYSIYRTTGPSITKLY
                     VPFFKVHVVDLGVYWLPIAIIYVATWSNAVNITDGLDGLAVGLVIFAVLAFSVLTYVT
                     SRADWSQYLGLPYIKQASELTVFNFALLGACVGFLWFNAHPAEIFMGDVGSLSLGGVL
                     AVLSLMVKKEILLLVIGGVFVVEIGSVMLQVVYFKLTKGKRLFLMAPLHHHFELSGLK
                     ETKVVVRFWIMGGIFALIALSTLKIQ"
     gene            complement(32231..33700)
                     /gene="murF"
                     /locus_tag="PK105_01200"
     CDS             complement(32231..33700)
                     /gene="murF"
                     /locus_tag="PK105_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOU60350.1"
                     /translation="MEDNSIASDQQVLFYAGELAGIIGATCKGELNTPICSVVTDSRL
                     VEPNSMFVALKGERTDGHHHILEAIKNGARTILAEIDEKDAAIASIRDSGLVAETCLL
                     LIDSPLSGLQNAAREYRRRQNLLRIGVTGSSGKTTTKECIASMLSACFPEESIAMSPG
                     NLNSDIGLALAIFGIEPRHKIGIFEMGINRKGEMDELAKMYEPEIALITNIGSAHVGM
                     FGSRDEIAREKSGIFSYFDGQQKALLWEDDDYRTYLSSKVKGSVYYFGLRSTEGLGRI
                     ENKGLDGWKIEWKGQTLDFRLPGKHNLLNACAALSIAILMELDPILAVQGLSKVQPLF
                     GRSEICKGKITVFQDYYNANPNSMAAAIDFLSGISSTNRKVLILGSMLELGKESDNAH
                     RDVGFMVAEMKPEAVFLFGEEMKAAEAALKEAAFRGVLMHTTDMGNLIDTVSSYVREG
                     DLVLIKGSRSMALERISHAFQEKGLIDLSEASKGGSHAS"
     gene            complement(33687..33992)
                     /locus_tag="PK105_01205"
     CDS             complement(33687..33992)
                     /locus_tag="PK105_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60351.1"
                     /translation="MNARRFWFSGIIICLLFIALVYQTTRYAALRAEVRELERAELDL
                     LKVGRELDADITKLANLERIERAAIRKGMIVATPEQRIIVTERQEQKASEVSNNGRQ"
     gene            complement(33989..34996)
                     /gene="rsmH"
                     /locus_tag="PK105_01210"
     CDS             complement(33989..34996)
                     /gene="rsmH"
                     /locus_tag="PK105_01210"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOU60352.1"
                     /translation="MIRTQKPYSDKESGMDFFLHTPVMLEQLCNLASTIKPMTLYVDC
                     TIGAGGHAEALLKKYEGLRCIGIDADPDACNRAAERLKPFADRLLIINGYYDEVLSDI
                     RNKRAGYCIPGDFSSCLCNENGESRFSLVLFDLGISMYQLRSSGRGFSFYADEPLDMR
                     LYPYSQCTAGELVNRLSEMELADAIFKFGEERFSRQIAHAIVQKRKKERIRTSSELAS
                     IISGAVPVKYRNGRIHPATRTFQALRILVNDELGRLERACIDALNLLSPGAVCAVITF
                     HSLEDRIVKNIFAEEAKNSHFSLEWKKPQSPTLEELRNNPSSRSAKFRAIRANAREEQ
                     K"
     gene            complement(35022..35465)
                     /gene="mraZ"
                     /locus_tag="PK105_01215"
     CDS             complement(35022..35465)
                     /gene="mraZ"
                     /locus_tag="PK105_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOU60353.1"
                     /translation="MKSLTGEFRNTLDEKGRLSLPARLRANLSSTLVLTQGVDPCLWL
                     FVPEEWEVLSQRILATTSLFQARSRLIQRRIIAPAQEVEVDKLGRIFIPQSLREWAGL
                     SKECVILAISRYIEIWDCQRYQAYMAEKQSEFLMAAEELGGLTLS"
     gene            complement(35635..36888)
                     /locus_tag="PK105_01220"
     CDS             complement(35635..36888)
                     /locus_tag="PK105_01220"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU60354.1"
                     /translation="MSEISLLNALLHGFDVVVRDPVWGDVHMDSSLHALFQSRAFMVL
                     DRIRQLGPVALVYPGATHTRKAHSLGVYHIARRMALALVEKGEIDFVSKEGLRSFLVA
                     SLCHDIGHYPYAHSLKELPLERHEALAGEMIISSPLREHILECGADPEQTASIIDPDR
                     TENKSRETLLFCSFLSGVLDPDKIDYLTRDAFYCGVPYGIQDADYIMRRLIVHDDRLA
                     IDLKGEMSIEALIFAKYQMYRAVYWHSVVRAATAMVRKAVYLGLASNVLIPSQLYGID
                     DTNFNRLMSSPRFPPFRLAREAEEKRVYKLAAEHVFDESNPLHRDLLDIVKRTEAESA
                     LAQAADIAENDVVIDIPERIKLETDLMVHVDNSFEPFDQRSTLFCPNIVEKVAEALRT
                     IRIYIRPIDGIDSSKIASLVDTLLD"
     gene            complement(36985..37467)
                     /locus_tag="PK105_01225"
     CDS             complement(36985..37467)
                     /locus_tag="PK105_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOU60355.1"
                     /translation="MNADLIRTIVLSILPVSELRGAIPYAVYKGFSIPIAAIISIAAN
                     ICIPIIAFLFLESLHRLLYKIPQYKRFFDRVVNRARAKVHTKVEKYGYWGLLLFVAIP
                     LPATGAWTGTLGAWILGLSHKKAFFAIAGGVILAGIIVSILVALWGVGAQTIFLRPVN
                     "
     gene            complement(37593..>38518)
                     /gene="miaA"
                     /locus_tag="PK105_01230"
     CDS             complement(37593..>38518)
                     /gene="miaA"
                     /locus_tag="PK105_01230"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU60356.1"
                     /translation="ASGKTHLLDTIFGEGADLWIPRLRSLWHADLNAAQVISADSMQA
                     YRFLDIGTAKPSKDLLKRLPHHLIDIKNPDEQYTAGEFVERADALCNQLSAQGILPIL
                     SGGTGYYLMNFICGLPTSPPSSRAIRAQVGRDLDEFGPQVLREELAEVDPNAAARIHE
                     RDIYRLTRAIEILRTSGKPPSLFAPKKAIRSGRKFIVIGITRSREELHVRIHERVEAM
                     MKEGLASEIEKLKSLGYSSKDPGLRAIGYREFFELEEDPIERISSAIELHTRQYAKRQ
                     MTFFRALPGIIWIEPYPADFYRVLKEIGSNS"
BASE COUNT        10611 a         9110 c         8277 g        10520 t
ORIGIN      
        1 tgcagcctcc attgtattag ccgtcggata tccctcctta tccggcggca ttttttatca
       61 tgaattgact ttttatactt atttctatat tgttactccg gtttttgaga aaaggagtcg
      121 ttatggcaca agtttctaaa aatgctcgca caggctcttc aactcaacgc ttctattgtt
      181 cttgcggcgg tgaaataaag atgaaaacca tctttgaaaa cagtaaggtc agaactatag
      241 ctgaatgcga aaaatgcaaa agagttgaga gacgcccaag cgacttcaag tgatctttaa
      301 atgatccgaa aagaagataa aaggccccag catattgccg gggcttttta tttatatgca
      361 ttgtcctgca gaatgttatt cgaagatgca ttattgtagc cgccgaagct caaggattcg
      421 ttcttcgatc aaacgtcgcg catcgatgag catgttttcg atatcatatt tattgcgtgg
      481 agcttcgtat atcttgacta tttcgacaat ataaggagtt ttgccaaata acttaagaaa
      541 atccatagca tttgctactc gccagccgcc ttccagagct atagcggcaa cagagagtct
      601 tccaccagac tcaagcaatt ttctgacacc tgcggtgtga aattctccta catgtccatt
      661 tctagacctt gttccctcag gaaaaatcac agggcaagca ccctcctttg cgcatatctt
      721 tgccattttt gacacttctc gcattgcatc aagaggagct ccttttctct ttatcaggca
      781 atgccctcct tttctcaacg taaagctcac aaagggaata ccatattgaa gctccttctt
      841 ggcgacaaag cgaggctttc gttcaattcc taagaaagct atgagcacca agatatccag
      901 tagactctgg tgattcgaaa ttacgagaca tcgaagagga atttcaagcc gctcgggatt
      961 atgcacccta atcctgaatc cacggaagac cctgaataac ccaaggataa tctttgcacc
     1021 aatttgctca tacttagcac aaagatgaaa gcgctcatat ctatctgcaa atagacacct
     1081 cagcgcaaga atatactcca ggagaagcaa catggagact atgatcaaga ctacgatacc
     1141 attgattagc attatcctaa atctttatcc tattttgcct aggaatgcaa ttccttcaaa
     1201 aaagtattga cattgatgct aggtatctgc tagctttagc acagaatttt gatcaaccca
     1261 agatttgtat taagcaatgc tattcggagg atttccattg agtacaacta acttgtctgc
     1321 agaaaagcgc cagaggcaaa atgaaaagcg caggcttgct aataaaagta caaagacttc
     1381 tataaaatcc gcggccaaaa aagtggtaat ggcgtcagaa aagaaagata ctgaagcggc
     1441 aaaacaggct ctatcagaga tgatcaagcg gattgattca gcagccagaa agggaataat
     1501 aaagaagaac actgcagcca ggaaaaaatc ccgtatgcag cgacttgtaa acaagataga
     1561 tcaataaatc agagagtata gtaccttcag gaaggagcag agaacgatat ggccgaaaaa
     1621 ttgacaaaag ctgaattgat agatgcgctg tatgattcat tgtcgtcgac aagccgcact
     1681 acaagaaaag aaatccatga gcttatcgac ggtttatttg ctgagataaa aggcgcaatt
     1741 cttgatggca agatagtcga actgagaggt tttggcactt tcgaggtaaa actcagaaaa
     1801 ggcagatcaa aggctagaaa tccaaagaca ggcgaaatcg tctctgtaac agatcacggc
     1861 gtagccacct ttaggcccgg cagagagctc aaaagagccg catgggaaat ggatatcgag
     1921 cgtatcccta aatcattcag aaagagagtt taattgctgt ccatttttgc taaataggga
     1981 tattgttaat gatggtagcc ttctatgtct atcatgaaaa agtcaaattt ggaaccttcg
     2041 gaaccgtacg caacgcatat cgggcctgcg ttaaaactga atggaattct acgttctaat
     2101 cagccccttg acttgcattg tgcttttcat ggttcaatag aaggaacctt cattaagatt
     2161 tccaagaatg ccataataga agctgatcta ttaaaagccc atcaagttgt tattgccgga
     2221 caatttaaag gatctatgag cgtttctgaa gaaataataa ttcttcctaa tgctaatgtt
     2281 tcgagcgata ttaaagtggc atgcctccat attattgaag gttcgcaatt tgaaggcaaa
     2341 atcgagatgg ctgggtttta tccagatcat aatagcccga tagtagatag aggacattga
     2401 catcgctcca ttgtcacact atattgaaat tgtctatcgc atatttgcgg cgcaaaatgc
     2461 cgcacttgct ccaaaaagca tacctttcga tttgactcaa tgcgccgctc aagaatccat
     2521 ataaagtaga cggggagcca taaagatgct tggcaccgtt ttgatcgttt aaaatcaggg
     2581 agattctcga tggcaattca caatagaaag aagatagatt catcccagac aacatcttcg
     2641 aataacgatg agaataattc cgcggagcca agagaggaaa tagccatgaa tcagagtcag
     2701 cacgaagagc aacccgtctt aaaaaacgag tcacttttcg aagaacagac tgaagatctc
     2761 gttcaagaca tacaaaggtc attgaacaat gtacatgtcg ccgcacctgt aaagaatcag
     2821 caacagaaaa cccgaaagcc agttgctaaa atccgtttaa agaagaagcc cactcaaaaa
     2881 gcaggcctcc tggaggaaac tcaagtacct gaatcgacaa caacttccga agtaatcgaa
     2941 gaacaaccca atggattcgt tgttgcagct atgccgctcg atgaacatca aaatcagaga
     3001 ctgtcggagt tacccaagcc ggagccagaa aaccgtacag aacagagagt agaatccaga
     3061 gctcgcttat tgatcaatga tctttcaaag atgggtataa tcgagttgag agaattaggg
     3121 gctaaatacg gtataaatca tgatttgctc atcacgctaa aaaaacaaga actcattttt
     3181 aatattctga aaggccatac cgagcacggc ggcataatat atgcctatgg ttcgcttgaa
     3241 attctccccg atggctatgg attcttgcgc tctcctcaaa actcttattt gcctggcaca
     3301 gatgacattt acatttctcc ttcgcaaatc aggctcttta acttaaagac tggcgacact
     3361 gtctatggtc agatcaggag ccctaaggaa ggcgagcggt tttttgccat gctccgtatc
     3421 gaacagatca acttcgatga tccatcggtg gctcaaaata gaattccctt tgagaatctt
     3481 acccctctct accccacgga aaaattggat ctggaaacca gcaccgaaga aatctctact
     3541 agaatcatca atcttttttg tccaatagga aagggacagc gaagcctcat cgtatctccg
     3601 cctcgtacag gaaagaccat tcttttgcaa aagattgcca atgctataac ggccaaccat
     3661 cctgaagtct atctcattgt cctccttatc gatgagcggc cagaagaggt cacagatatg
     3721 gaacgaaccg taaaggctga agtaatctct tccaccttcg atgaacaagc tacccgccat
     3781 gttcaagtag ctgagatggt gctggagaag gccaagcgtc ttgttgaaca caaaagagac
     3841 gtagtaattt tgctcgattc gattacaagg ctagcacgag cctataacca aactgtcccc
     3901 acttcaggca agatactgtc tggtggtgtc gattcgaatg ctctacacaa gcccaagaga
     3961 ttcttcggag cagcacgaaa tatagaacaa ggcggttcgc tgactatcat agccaccgcc
     4021 cttatcgata ccggttctag aatggacgaa gtaatctttg aagagttcaa gggaacagga
     4081 aacatggaga tcaacctcga tagacgcttg tctgatcgac gtctctaccc tgctattaac
     4141 attaaaaaat ctggaacaag aaaagaagag ctcctgatct ccgaagaaga gcttcaaaag
     4201 atctgggttc tgcgtaaagt aataaatccg atggatgatg ctgaaataat cgaactgctc
     4261 atcgatcgca tgactaagac aaagaacaac gacgcatttc ttaaatcgat gaacacgcct
     4321 tattcgagca tggaataggc ttacgtcgta ttgactagag ttgattgatt ttatatattg
     4381 aatcgctata tctggctgat cttcttacag tagttttacc agggctctat ggaggaataa
     4441 atgaaaaagg gaatacatcc aaaatatgat atcacaacaa taacttgcgc atgtgggaat
     4501 gtaatcgaga cccgttctac agctaagaac atacaagttg aaatctgttc tgcctgtcat
     4561 ccattcttta ccggcaaaca gaaacttgtc gacactgcgg ggcgcatcga gcgctttaac
     4621 aagaaatatg ggataaagac taacgaaaac aaataatagc ttgagactca gatgcataaa
     4681 ggctgccatc gaaaaaaggc ggcctttttt attttcaaag ctaagagcga gctttctcag
     4741 tctctacagc caaggctgag ggtagctaac atcgttgcca ttctttgcgc cgccttgcct
     4801 ctatgcgaaa ttctattttt ttcatcagcg gatagttctg cgactgtctt cccatactgc
     4861 ggaaggtaga caatcggatc ataaccaaaa ccgcccttcc cgcttggact tccggcaatt
     4921 tcgccttcta gagtctcctg gacacacata aaacgctgct ttccataata gaaaacaaga
     4981 caacaaacaa aagcacagtt cctattggaa acacccgcaa gttcggagag caaaaggcta
     5041 ttcttctctg aagctgcaag aagctctttg ccattagaac cataccgggc ggatcctatg
     5101 ccgggctttc cgcccagggc ttcaacgcag agccctgaat catcggccat agatggtatt
     5161 cctaatagct cataaaggct tcttgcttta atataagcat tttcaaaaaa tgtcttccca
     5221 ttttcttcgg ggcagaattc attgattccc agatctgaag gcattagtag ctcgtggcat
     5281 ggaaacaggg gcttaagttc aacgatctta tgcaagttgt ttgtggcgac gacaaatctc
     5341 atagggtttt tttagcctag ttttctataa tcttcaagac tcctacctga atcgacatgt
     5401 tttctgagat aaaaaagtcc tgagtcaatg ctacccttcg gaaccccgag tatttgcgaa
     5461 attgattcat tggagacagc cgatctgcga tggcagcgct tttccaaaag cctggcatag
     5521 cgctctcgac atcgttttag agagcataga atatattctt tttcttcatt aagaagacag
     5581 gtctcgagtc tgaattccaa cacgcggagt cgaatccata gtgcatgcaa tttgatattc
     5641 atttgctctg ctttattcca gcctctctcg gttcttcttt ttgtcaattc gattagattg
     5701 aaaaggtatt ctctgggtat acccagagaa agactgcatc tgctcaagag ttcctcatct
     5761 atctcccaag cgcatttcat tgttagaaaa agcagtctct tgtgagcagg gctcaattta
     5821 tccagaatta ctttgggagg aagatttaaa attcctttct ctgtggttgc atcatttatt
     5881 gtttcataat atgtccacgg ttcaaccgga gattcaattg cgagttcttc tgtgaaattg
     5941 gaatatatat aagcagtttc acaaagactg agattataat tttgacgatg tacactcttt
     6001 gcgacatatc gcatactgct cagcagatat gcatcttttg ttctgctttc attttcagcc
     6061 cgttcaagta taagctcaat gcggttgcga taattattga gcgcatcgga tatttcatcc
     6121 tttgtgaaaa agccatagcg ctttggatgt tcatacatct tgcggacaag atcgaggtag
     6181 gtttgagaat ccattcttga gcctccgatt ctaatcagca aacgatgtgc caaattttaa
     6241 aactaagcat agatgaaaga gcaattttta ttttactatt taatcaggaa ttatttgata
     6301 ttacaaaact cgattgaaaa caaatcaagc ttgaaatatg ggatggaaag caagttcttg
     6361 attattgctg cgcagtactc atttatagaa ggcaaaaact cggtaatact attgactggc
     6421 gaaaataata gttaaatctg aggatattgc tttctcaggc gaaggacttg ccttttcata
     6481 caaagggcgc ttgcgcaggg tatttccccg tccttctcat tggaaagaat acactttccc
     6541 tgaaaaagga caattagcaa tgagcgctta attatgctta tttcggcaca agcaggttgt
     6601 ctatcctgcc ttcacgcaat tcagtctcta tttttcgatt tcttcgtctc tgaaaacgtc
     6661 cccaaatact ctgactagcg tgtggacgac ctcatccacc ttgccattga tagaaaggcc
     6721 agcggcaagc cgatgcccac cacctccata ctgttcggca attctagcaa cattgatgga
     6781 attcttagac ctaagaccaa cggtacacat ttcctcggtc tcttgtttga ctacaaaaca
     6841 gacctcgcag tcgactatgc ccatcattag ctgatatagc aaatcggagt ctctgctgga
     6901 catgccatat ctctgttgat cttctatggt aacccaagaa actagaagtc tgccttcgta
     6961 gtatggctga atacgaagca gaagctcacc aagcatgcgc cgagaagcca gagtccttcc
     7021 gccattgatt gccatataca cacgcttagg tgaaacgccg gcttctacga gccttgcggc
     7081 tattttaagg ctctcacttc catgttcatc taaatgtcga aaaaagcctg tatctgtgca
     7141 taagccaaaa aaaagtagct ctgcttcttc tctggtcggg ctatgaccca ttgcctccat
     7201 aagaagcaaa atcatagccg atacggccga agccgattcg tccaaccaat catatgggcc
     7261 cggagcactc gatgtcgaat gatgatcaat gaatgaagcg ggaatattgg gcatcctctc
     7321 ggcgacactt cctattcttt ccatcgatga gcaatccagc acaattgcag ccgttctctc
     7381 aaaatccctt tcgcttggga cctcgctttt gaagcgcgcc tcaaaccgga gtatttcgat
     7441 cctattgaat ggcccggaag acagaacatg aacgcgcttt cccatgctct ggagcataga
     7501 agcaagtgcc agttgggaac cgatacagtc gccatcaggt tcgcgatgac ccagtatata
     7561 aaaacaatcg tgatgattta tgaagtctat aagtatcttt ggaacttgtc tcatagcaga
     7621 tattattgat tcacatccca gatgctacag cgtagcaagg attatggcct ttatggtgtg
     7681 ttttctattc tctgcctctt cgaacacctt tgattgaggc ccttcgatga catcgaatgt
     7741 cacttcctcc cctttcactg ccggtagaca atgcaagaat atgcaggcct tatttccggt
     7801 ctttgccata agttcagcat ttacctgata tggggagagc attctgattc tttcggcttt
     7861 gagggcttct tcccccattg aaacccagac atcagtatat atagcatcag ccccattgac
     7921 cccactctct atctcatccg tgattattat ggtagaaccc gatgtcttag caaagccctc
     7981 gcagaggttc tttagctcat tatcaggcca gagctcgcgt ggcgatacaa tggtgaaatg
     8041 catgcccagt tttgcgctga ttaccatcag cgagcgagct acgttgttgc gtccgtcacc
     8101 gataaaacag agacgctttc cggcgcaatt gcctaaattc tcctcgatag tcaagacatc
     8161 ggcaagagct tgtgtcggat gaaataaatc tgtcaggccg ttataaacgg gcacacctga
     8221 gtatttagca agaatctcga catattcttg tttatagcca cgaaactcga tggcatcaaa
     8281 catgcggcca agcacgcgag ccgtatcttc gatagattcc ttggctccaa gctggatgtc
     8341 ctgattagag agaaagacgg gatgcccgcc ttcttctcca aaggctgtct cgaatgcgca
     8401 acgagtgcgt gtagagcgtt tctcaaaaag gagagccata gtctttcccc taaaacgctg
     8461 cttatgcatg ccttttttcg cttcttcctt gacttttttc gataaactca aaaaatacct
     8521 tatttcttcc ggtgtataat cgatccatgt gaggagcgat cttcctttca gaatagatgg
     8581 attcacgagt cctccctctt attgctatta aatagagccg ccatatacta tatggtgaat
     8641 tgagaaaggt caagtttgcg ccaggagaga gtaatgagca atgaactgga aatatcccta
     8701 aaagaaataa atgaatctat tccccttcct cagttgctag aagagttata cattcgtctt
     8761 gttccgatat ggcctgacac tcgccctctt ttgcattata acaattgctt tgagcttctt
     8821 atatccgtga ttctcagtgc tcagaccacg gatgatcagg tcaatattgt aacgaaagag
     8881 ctttttcatc gctttccgaa ttccgagagc cttgctgccg cagatataca tgaggtagaa
     8941 aatatcattt accctctggg cttttatcat gtgaaagcaa agcatataat cgagaccgca
     9001 aaggttctcc aagagcgatt caacgggcaa attccatcta atttcgaaga aatgattgaa
     9061 ctacctggcg taggaagaaa atctgcaaat ctgcttatag cagcctgtta tgaaaagcct
     9121 ggtatcattg tggatacaca tgtgcttaga gtgctcttta ggctaggggt atctgcaaag
     9181 aaagaagcga ctcttatcga atccatcatt cgagcgaacc ttccgccaga aaagcataca
     9241 gaattctcgt atgctgtgaa taggcacggt aagttcactt gtactgcacg caagccagcc
     9301 tgtacagagc aaaaaaagca ctgtcccatt gacgatatct gcccaaagat aggattatcc
     9361 gctttattga agcaaaggcg ttgaatagag cgtctaatag ttgaaagcta aaaatgttca
     9421 aaccggatct cgtttctgaa tctgaagacc ctattggaat tctagggcgc gatagatttc
     9481 atatcccaca tttgacgcct ctccagcgct ttgtgattgc gaatatattg gatgagacta
     9541 aaggctccgc gatgttgacc agaaatctaa gttcggatcc tatagacgat gacgcagtct
     9601 caaacctgaa taaagaaaat gaattccaag aaagacagga aacctatcgt cagcttatcc
     9661 tatttccgac tggatttggc aaatcagtct gctttcaact accctcgctg cttttggatg
     9721 gactgactat agttgtctat cctcttcttg ctcttatgaa tgaccaaaag cgccgactcg
     9781 atgaggccaa tatttcctgc gctctttttc gcggaggcct tagccaagaa gaatggaatg
     9841 aagaggaaaa gctggtctct tcgggaaaag ctaatatggt cgttgcaaat cctgagatct
     9901 taagcgcacc aaagctgcgg cgattcttga atcgattcaa aattgctcac ttcgtagtcg
     9961 atgaagccca ctgtataagc gagtggggag agagttttag gccttcctac cttgctctcg
    10021 gtgagactgt tgaggcaatt tcgcctggga ttttgagtgc ttttactgca acagccggcc
    10081 ctcatatcgt gcactcgatc gagaagagac tcttcatgga taaggattat cgattagtag
    10141 attcagaggc agatcgttcc aacattctct atgcggttgt gcatacactt tctgttacca
    10201 ggactctccg tcagaccctt ttgtgctgta aaaaaccggc tattattttc gatcgttcac
    10261 gtccgggaac aagactaaaa gccgagctgc tcaaagcttc tggattttct caagcgcgct
    10321 tttatcacgc tggcctcgat cgagccgaac gctgcaatat tgaaaaatgg tttcaagact
    10381 cagacgacgc aatcttggtc tccacaaatg cctatggcat gggcatggat aagaaaaaca
    10441 tcaggactgt tgttcacagc tcgctgccag aaagtgctga agcatatatt caggaagccg
    10501 gtcgtggagg ccgtgacgga aagaatgctc tagctgtgct catagatgat cttgcggtgc
    10561 gatattacag agaaaacctg aaggataaga acatccaaga catacagtcg attcgaaaag
    10621 cttgtttttt gccctatccc actttgagtt catgccgacg agagtttctg cttcatctct
    10681 tgggtgaaga aaaaactcca gaatgcggga gatgcgataa ttgtatcgaa ggatcacata
    10741 ctcagagaac gcaattcaat tcagatgcct cagatatcct tccggaatgg aaattcttgt
    10801 caagagcaga agggtttatt gaggctattt ctttctgtga ctcacaccat ggagttttta
    10861 cttcccaaga actagtcgat gcgctggggc ctcatggaaa aggaaaaggg gaatatgccg
    10921 gctgtctata tggatggcta aagatagaaa gagaagaatt gatcaatgca ctattcttac
    10981 tgaatatcct ctatgcaccg aaaaagggaa cccggaagaa caggattcta cttagccaaa
    11041 aaggcaaagc tctgagcaga cttgctcgca acagccttgt atgagcttga agtgtatcgc
    11101 tattagcaat atcatgtatg agaaagaaac acctcaacaa gaaagacttt gaatcgaggg
    11161 gtcattatgg ttgttttcaa aaaccttgat acatgccagg cctttgaagc cctaaagaag
    11221 attccggccg tcgtattgca caaatctctt gaccaaaagc gaatatcgga ctggtggatc
    11281 cctcacgccg caggtttttg ctactgctat gcttttgcgc cagtagacga ttcggtcata
    11341 gactggctcg aagcactgac cagggaacaa cagctcatag aaaagtatcg catactgaca
    11401 gaaggtgcga ttatgaacac aggcgaaaaa aggatggtgc tccatcactt ggttcgagga
    11461 cgcctgggca agcctgttat ctggaatggc caggatatgg aggttttcta tcgagaagaa
    11521 aagcatcgat tctacgattt tgcacataag attcgagaag gcgaaatcct cggcactaca
    11581 ggcaaggctt ttacgcaaat tgtacagatc ggcataggag gatcagatct aggtccaaga
    11641 gccgcgagcc tagccctttc tcgctgggca gttgcagcag gacgggctag gctcagaccg
    11701 tggtttattt cgaatgtaga tccagacgat gctgattatg tgctagcaaa catagatctc
    11761 tcagagagtc tgtttatact cgtctcgaag agtggaacca cacaggaaac gctggcaaac
    11821 gaaacaattg tgcgcgacag gttgcagaag gccggactta atccggataa gcatatggtg
    11881 gctgtgacaa gcacttcgag ccccttagct cataaccccc attatcttga tagcttctat
    11941 atagacgatt tcataggcgg acggtattcg acatcgagcg cggtaggagg ggtgatcatt
    12001 tcgattgcct atggccctga aacctttgaa gagtttttag aaggagcaaa tgaagctgat
    12061 agattagcct ttgaaccttc cttgagaaag aatccagtac tcatggatgc tttgatcggc
    12121 atatatgaaa gaaatatact gggttggcca gccacggctg ttttgcctta ttccgaatcc
    12181 ctctctcgtt tccccgccca ccttcaacag ctcgacatgg agtccaacgg caaatcagta
    12241 aatcgggaag gtcatcctat ttcttactca accgggcctt tagtatttgg agaacctggc
    12301 acaaatggcc aacattcttt ctaccagctt ctgcatcagg gatcggatat tattcctctc
    12361 caattcatag gttttctcag aaatcaactt tcgccagata tgatttctga gggttcgagt
    12421 tcgcgtcaaa aactcttggc caacctcatt gctcaaatgc ttgcatttgc catcggcaaa
    12481 aaggatgaaa atccaagcaa aactttctca gggaaccgtc cctcgacact tctattggct
    12541 agagaacttg gcccaaggca gcttggcgct ctttattccc atttcgagaa taaggttatg
    12601 tttcagggtt ttgtgtggaa tctcaatagt ttcgatcagg agggtgtaca actaggaaaa
    12661 atccttgcaa aaaaggcctt gagcgcaatc accaaaggtg aatccactga tgatgaacta
    12721 ttagatgctt tcattgcttc tgctttgaga gctgtcgaga ataatggtga ttgagtcagt
    12781 tggataatgg gaatcaatga ccaatgggag aaggtaaaat gcagtctatt tatgaaacag
    12841 agcttcagga gaaggatgag cttgagaatg actgcatcgc ttttacgggt ggaggaacag
    12901 gaggtcatat ataccctggc ttggcggtta tagaagaatt gcgccgcctt gggtttaagg
    12961 gaagaattgc atggataggc tccgaaaaag aattagatcg cagcattgtt accggacaag
    13021 gtatagagta tcatgcgatt ccttcgggga aatttcgtag agagctctcg cttcgcaata
    13081 tctctgatct gggcagggtt gcctcggctt atttcagagc gcgggctctt ttaaaagata
    13141 tcggtccatc gatcctattt tccaaaggag gctatgtgag cgttcctcct tgctgggctg
    13201 catcctccct gggtatcccc gtaattaccc atgaatcaga tgcatcacct gggctggcaa
    13261 cccgactcaa cagcaagaaa gcatcgctta tcctgacaag ctgggaggtg acggctcaat
    13321 actttcctga gagtctcaga accaagatca taagagccgg aaatccagta cgtcctgata
    13381 tcttgaatgg agatcgacag cgcggactat caatgctcga atttgtagct gaaaagcccg
    13441 tattatttgt gctaggtgga agccagggag cacaagaggt aaacgatatc gtactttcct
    13501 cgctcaatct gcttgtacct ttggttcaaa tagtacacca aacaggtagg gctaatttcg
    13561 atgaagtcaa ggcgagagtt ccggatgatc cagccgtgcg cagagcatac cgtccttttc
    13621 cgtacatagg ttctgaaatc gcagacatct atgcggcttc gaccatgata gcaggaaggg
    13681 ctggtgcagg cacgatatgg gaggcggcat cgcttggcaa gcctatgatc ctcattcctt
    13741 tatcgggatc tggtactcgg ggcgaccaag tggaaaatgc agctcttgct gaacgttcag
    13801 gcatagcgac tgttctgctg aagcccaata tttcatcgaa tttatttgtc gatgcagtca
    13861 aaaaatatct gaatatcaag aattatgcca tggcagaaga ggcatgccgc aatttagtgc
    13921 acattgtcaa aactccccat attcctgaaa ttacatatgg tcctagccaa aatccaatat
    13981 tatcagcgga atatatagct cagctccttc taacaaagat ggcagaatgg gcaaaggaat
    14041 ctgcatgagt acaatagact gggtttttag cgggattgta ataatactcg cagcacgttg
    14101 ctttgttaga ggctttatcc atgagattct atctgtagct tccatcgcat tgggaatcct
    14161 tgctggctta ttgctttcaa acactgtcat cctgcagata ttgccgaaga tcaacgccga
    14221 caaacttcct tatcaagctc agtatatcat cgcctttatt ctttgcttca tagcagggtt
    14281 tcttctcatg aagatcatag agcgcatgct tcgccaaggg ctcgaggcat ccaaccttga
    14341 catactcgat agaatcctag gccttattct gggtgttgcg gaaggtttta ttgttgtggg
    14401 tctctttatt gttattctgg agatacagcc tattgtagac gcaaaggcaa tactctccaa
    14461 ttcgctctat gtaaaacttt tgggaccaat cgtcgaaccc gccataggtg gaactctgag
    14521 cccaatgcta aaggatatgg atatgccaat ccttaagcag aagcccaaag gcaaatgatc
    14581 atgtttgaga acctgctttt tcaagaacat gctttagaac agcttgtttc gctggcaaag
    14641 gaaaagaagg tgccacccgc ccttctcttt gttggtcctg ccggatccgg aaagctgaca
    14701 gcagcccttg aatgcgctcg tgttctttct tgcgaaaaag aagcggaatg gaattgcgaa
    14761 tgtacacaat gtcgttgtca tagaaacctt atgcatcccg accttttgct ctttgggcct
    14821 agagaattcg cccaggaaac catcattaca ggagaatacc tgatcggcaa gcctggcctg
    14881 acttcatatt atgcctttgt gcgctcgata aggaaactcc taaaacgctt tgattcagtt
    14941 ctttggcagg gagaagaagc aagactatca aaggctatac catccataga aacctgcgag
    15001 gagctgatac aagagatttc tgaacttgtg ttgaagaaaa aaaatcacgc cctgactgta
    15061 gcggtagaaa atgccataaa tgcggcagct ggactagaat ctctggtacc agatggtatt
    15121 ccagttttta tgatcagaaa catgatttca tgggcagtgc ttgcgccatc tgcaaaaacc
    15181 aaaatgatca taattcaaaa tgccgatgcc gctaatgaaa gtgcgcgaaa cgcaatgctc
    15241 aagattctag aagagcctcc agagacagtt cgttttattc tgacttcatc tcgccgcgct
    15301 gctgtcattg caaccattct ttcaagatca aggctcatat cgtttgactc tcgaacgggt
    15361 gatcaagcgc gcgaaatcat ctctcgtcta tttcatgtag atgaagacct gcaaaacatt
    15421 aacagcataa gtgaattctt tcagaataag agcccttatc cacctaaggt cgccacccga
    15481 gatgcagcct tatttatagg tgctttgctt ggaaaaacta tgaaagagaa tcctggattg
    15541 agtggaaaat atgctattga acttgcctcc atggcgcaat gtgagaatgt ctccgctcta
    15601 tcgattcttc gccaagtgtc agacaaaacg aatgcctttg gtgccaagaa ttctagattc
    15661 gctaattcat ttctcgtgtt catgagagcg gtttcacagc atctggcgca tatagctgct
    15721 gatccaagct cgagcccttc tcttatcatg tatgtagatc gcctcttttc caagctgagg
    15781 gagatgaaca tgcaatatcg tacattgaat cggtcgccag aactcatgct tgaagcattt
    15841 gccgatatac ttggagagca gtatgaaagc cgcatatgaa aacactctta agcgaatcga
    15901 tcgtattcct ccggagggtc tcaaacagat agtaagaaac ctattagcag agaactcgat
    15961 ctatgaatct gccctaaatt ctacgctcga tgggatcatc gtatgcgatc tagcccacaa
    16021 accgctcttt ataaatcgct cggcagaaaa aatcacaaga atcgtatcat gggcacaaga
    16081 agttgtactt tgggaacagc tcgatgatga caatctggct gattttatcc aagaagccct
    16141 ttcatccgaa aagaatatat tgaatcacga actctctatc agcaggcctg gagggacgag
    16201 gctgttatcc atcagcatat ctgcgctggt atcggaagga aaaatagcgg gcaacctaat
    16261 tcacatcgag gacattacag aaaagcgccg ccgcgaagct cagctccgta gggcagaaag
    16321 cttggctgct cttacaaccc tagcggctgg agtcgcgcat gagataaaga acccccttgc
    16381 ctcgatccga attcagcttg gcattattcg acgcatactc gaaaagaatt gtgccaaaaa
    16441 aacagagcct atctttcata acatttcttt agttgagcag gaaatagatc gactcaattc
    16501 gattgtagtc gattttctat ttgccgttcg tcccatggat ataacgctca ttcgtaatcg
    16561 tgttgaagat gttatcaagg aagttgttga aataatgggc aatgaggcct ctgtccatca
    16621 tatcaatatc gtgtgcgaat tcgaagataa tcttcctgaa gttatgatag accctaagca
    16681 tctcaagcag gctctgctca atattgtcaa gaacgctatt gcagccatgc ctaatggtgg
    16741 aactcttact atctctgccg tgacaaggca cgacgaactt caaatatcta tttccgatac
    16801 aggcatcgga attccagaag aactccttgc caagatattc gaaccttatt ttactacaaa
    16861 ggaaagcggt acaggcctag ggcttacgat cacattcaag atcataaaag aacacaatgg
    16921 cgaaatcacc gtggaatcca agccatcaaa aggatcaacc tttacaatcc atttgcctat
    16981 acctcaacct gagcagaaat ccctgccacc ttgggaagag ggtctacaaa ttgaaggctc
    17041 ggagagggag gaatagatgc gagcaacact gttggttgta gatgatgaga tcaacatacg
    17101 cgaaggactc gcccagcttt tttccctgga aggctacgat gtaaaaactg caaaggatgg
    17161 cttagaggga aagatgatcg tcgataagga agatatcgat gtagtcataa ccgattacaa
    17221 aatgggaagg atgagcggtc tcgatcttct gcagtacatt gttagcacac atccgggcat
    17281 acctgtgata atactcacgg ctcatggtag tgtcgacaag gccgtggagg caatgagaga
    17341 aggcgctttc gatttcatcg ccaaaccacc aaataccgat catctaatca atcttgtccg
    17401 ccgcgctctg gagacaagag agctctatcg aaccaacatc gagctaaagg cagaagtcga
    17461 aagccagcga gctcggagct acatcattgg taaagccccc tccataaaac gcattttcga
    17521 cctcatacgc aaagtagccc caaccaaagc aagcgtatta ataacaggcg aatcgggcgt
    17581 cggaaaagaa cttgtagccg acgcgattca caacttatca ccacgcagag acaagccttt
    17641 tataaaggtg cactgcgctg ctctcgccga gagtcttctc gaaagcgagc tcttcggaca
    17701 tgaaaaaggc gcttttacgg gtgcgaccgg cagaaaacga ggacgcttcg aaatggctga
    17761 cggaggaacg cttttcctcg atgaaattgg cgaaatcaat cagaatgtgc agatcaagat
    17821 tctgcgtgtt cttcaagagc gaaaattcga aagagttggc ggcgaagaga ctatagaagt
    17881 cgatgttaga ttgatcgctg ccaccaaccg agacctcaaa agagagatca ccgaaggccg
    17941 ctttagagaa gacctatatt atcgcctgaa tgtagtcaac atccatgtgc ctccattacg
    18001 cgaacgcaga gaagatattc cgcttctcgc catggctttt ttgagagagt ttgcggatga
    18061 gaatggcaaa cagatcgagg gtttcgatcc aagagtcaga caggctctct acgcctacga
    18121 ctggccagga aatgtcagag agctgagaaa ttgcatcgaa agcgcagtgg ttatggcctc
    18181 cggtaagctg ataacgctcg atgaccttcc tccggggccg cgctcgagcg gagaagctcg
    18241 cacaatcagc ataccggcct tttcatctct ggaagaagcc gaacggatta tcatttctga
    18301 aacgcttgct cttgtaggag gaaataagtc taaggctgcc gagatactta aaataggccg
    18361 aaagacgctt tatcagaaaa tagcgcaata tgggattgga aaacaagaag ccaactcttc
    18421 ggtgtcttcc aacccgagag aagaaacaga gaattgataa gatccttgat acaatatgag
    18481 catactggct atattatgcc tcgtttttta ttcgatagtt tggcacgaag gcgtaatatg
    18541 gccttggtat ggagttggct caccctagat tctgttacct caagtatctt gcctatttcg
    18601 cgtaaggtca gattctcata gtagtatagg accatgactt ttttctcctt ttcgggcaac
    18661 tcctggagag cctcaaatat gactcttcga atttcctcgc gttcgagcac caagtctgga
    18721 ttttcaagtc gcgtcgattc gagagaatct tctatagtat atccctccgc ttcggaatta
    18781 attggccaga tctcatgcag agaaagaatc gaagaagacg ccactttaga cattatcttc
    18841 aagagttctt tgcaatctat ccccatctcc tttgctattt cttcatcggt ggcgggtcgg
    18901 ccgagctgac tctccaattc agcgatagcg cgctctatct gcttcgtctt tcgacgaatg
    18961 gaacgaggaa cccaatccag ttctctgaga tgatcataga tggctccacg tatccgtgtg
    19021 accgcataag tcttaaattt tacattcttg tttggatcga atttttctat tgcatcaaaa
    19081 agcccgaatg ttccataacc gacaaggtct cgatattcca ccatcgaggg caaagtcaca
    19141 gatacttttc ctgcgacata cttcacaagg ggggcatagc gcatgatgat ctcattgcgg
    19201 atatggatat cttcagtccg tcggtaaagc tcccataaac gatcttcttc taatgttgca
    19261 tagtccatga tccgcccttt ccctttttcc attaaaggat atacttgctc aaatcctgct
    19321 gttggacgta gtccttaaag cgttcgtcca cgtatgctct atcgatgaca acttttgtgc
    19381 ttgtatgctt gtcggcatcg aaagaaatgt cttctaagag cctttccatt accgtctgaa
    19441 ggcgccgcgc acctatgttc tcgctttgcc tattggctgc ggcggcgatc tcggcaattc
    19501 ttgcaatagc atccccattg aactcaagct gtacatcttc cgtgctcagg agttgaatat
    19561 actgggttac aagagcgttc ttaggctcgg tgagtattct cacgaaatca tcgccagtca
    19621 aagcatctaa ctctacccta attgggaagc gcccttgcag ctccggtatg aggtcctgag
    19681 gtttagccac agtgaaggct ccagctccaa tgaataaaat atgagttgta tcgattggtc
    19741 cccattttgt gttgacggtc gtcccttcga cgatgggtaa aagatctcgc tggactcctt
    19801 ctcttgagac atctatgccc gaagagcctc tagcatcacg gttcgcaatc ttatccatct
    19861 catctatgaa aatgatgccc aactcctgag tgcgtcgacg agcctcctgg acaaccttct
    19921 cctgatctac aagatgttct ttttcttcgg cttcaagaat aggtaaggct tctgcgacac
    19981 taaccgtctt tttctttttg ctaccaccaa ataagccttg cagcccgccc aaagcgatat
    20041 tcatctcttc gaactgcgtc ccagcaaaga gctcaatttg tgggccctga gcgggcaccg
    20101 taatttcaat ttgtcgcgaa tttagtttgc cttcttttaa gagagtgcgg aatttttctt
    20161 tggtttccat tccggaatta ggaattatca atgttgcttg agattgccca ataggctcat
    20221 cagattttgc caggcctggg agcaaaatat ccaaaagtct ttcttctgcg cgcctcttcg
    20281 cttcagtcgc gacttgggag tccatttcct ctttgaccat cgatacggct gctgccatca
    20341 gatctcggac catagattcg acatcccgac ctacatagcc aacttccgtg tattttgtgg
    20401 cttcgatctt aagaaaaggc gcattacaga gcttggcgag ccgacgagca atttcggttt
    20461 ttcccacccc ggtggggccg atcataagaa tattctttgg agcaatctct tcccggattt
    20521 ctggaggaag acattgtcgc cgaattcgat tacgcaatgc aatggctacg gccttttttg
    20581 ctttttcctg gccgacgata taccggtcta gttcttcgac gattttatgg ggagtaagcg
    20641 catctttctc tataatcata tctcctccac gataatctga tcattcgtat atatgcatat
    20701 acttgaggcg atctttaggc ttcgttccgc gatctcgcga gcggagaagc tatctgggag
    20761 cattcccgca tcctcgcctg gggtggatct tgctgcttct ttcattctgc gagaagcatc
    20821 gaggtaagca attgcggcag cgtatgcata gctgcctcct gaacctatcg caatggcatc
    20881 ttcgcttggt tccaccacat ctcctgttcc agaaagcaaa aacatcttgg attggtccgc
    20941 aaccaagagc atagcctcca gcctgcgaag tacacgatcc gtgcgccagt cttttgccaa
    21001 ctcaaccgaa gccctggtca gatcgccacc atactcctgc actttggctt cgaaatgttc
    21061 aaatagggta aaggcgtcag ctgtagctcc tgcaaagcca cacagaactt tcccattata
    21121 gattgtgcgt attttgcgag cattgctctt gagcacagtg ttattcatcg taacctggcc
    21181 atcaccggcc attgcaacat gtccatcttt tctgacagcc agtatcgttg ttgcatgcag
    21241 ttccatattt ccctctcttt ctcttatttg gtctttgaac cgtgaggatg cgcctgctca
    21301 taaacccgac gcaatcgctc catgtcgaca tgagcataaa cctgtgtggt cgagatattc
    21361 gaatgaccga gcatctcctg tacaacgcgc atgtcagccc cagcagctac taaatgagtg
    21421 gcaaagctat ggcgaaaggt gtgtggcgtc aggtgttttc tgatgccagc tttgagacgt
    21481 cgattttcga ctatcttttc aacaccacga gaactcaatg ggcgacctct cgaattgaca
    21541 aagatcctat catgttcttc gatgccatat tttcgaagaa ttgccgctct ataaggcaga
    21601 tattggccga gcgctgtatt cgctgcatcg cataggaata caagacgctc cttagaaccc
    21661 ttgcctttta ctcggatcgt gcagttgttc gtgttcaatc tcgaaagtcg cataccacat
    21721 agttccgaaa cgcggctgcc cgtggaatac ataacctcca aaagagccct gtccctcaca
    21781 tccagaaaag tatctccccc taaaccgtca agaagactat tgatctcttc ttcaaaaaga
    21841 aatgatggaa gagttcttcc caccgtaaca ttttctactt ctcgtgcagg atcttttcct
    21901 tttttatgaa acttcatcca atatctataa aagccgcgta ccgcagataa agccctgttg
    21961 atagatgatg cagctctctt ctccatcaca agagtaccgg caaatgttct aatatcactt
    22021 gcatccgcct catccaggtc ctttccttcg agaaatatct cgtacttcgc gagatctcgc
    22081 ctatatgctt cgactgttct aatcgaaagg cctcttacag aagcgagata atcaaggtag
    22141 tccaaaagtc gcgcatccat gattcatgac tctccgttgt tttctccact ctgttgactc
    22201 gagtggagat gatcacagtc tggatttatg caaagtttgc gatgaccggt ttttttgtcg
    22261 tatttctcaa caagaaacca gccacatttt gggcacaaag aattgcttgg cttataaagg
    22321 gttttaaaat tgcattcagg atagttcgag cagccataga atttctttcg cctacctttg
    22381 ttagagacac gcggaacaat atcgcccccg cattttggac aggtagccag cggcactgat
    22441 ttagtaaatg tgcattctgg aaatcctgaa caagatataa aatacccaaa cctgcctagc
    22501 ttttttacta gaggacggcc acagatcgga cacttctcat cggtcttctc gtccaatgca
    22561 ccatgcatat cttccaggac gttcaaggcg ttttcgactt tatccaccaa tgggaaatag
    22621 aactttttga gctcacttac ccaatcgatc ttctgctcct cgactttatc gagcatggtt
    22681 tccatttccg cagtaaagct ggtgttgatt acttccggaa agttctggga aagaaggtca
    22741 tcgattattc tgccaagctg cgttgggaca agctggcgtt tatctctgtg gacataatat
    22801 cgctcgatga gagtctcgat tgttggcgca taggttgaag gccttccaat tcccagctcc
    22861 tctagggttc gcacaattga ggcatcggtg tagcgcgaag gcccttgcgt gaagtgttgt
    22921 tcgctttcaa ttctttcaac ctggagagtc tcacctttct cgaacggaag atgatggctt
    22981 gctctttcct ctttggaagc cgcgaggcga attaccttat aaaagccctc ctctaagaag
    23041 ctagaggctg aagtacgaaa aataccatca ccaattaaaa tatcagcagt caaggttcga
    23101 attattgcag gcttcatttg agaggcaacg aattgttccc aaatgagagt atacaatttc
    23161 aactgatctc cttttaggta acgccctgct tcatcgggag taatgtccac tcgagtcggc
    23221 cggattgctt cgtgagcgtc ctgagcggca ttgctgaccg aatatctaat aggcgcagct
    23281 ggggtttgat tgggaaagta ttttgtgagc cattcgtata tttcatggag agcggcctca
    23341 gaaattcttg ttgaatccgt cctcatgtat gtaatcaacc cagtccgctg atgccccata
    23401 tctacccctt cataaagctg ctgggcgatt ttcatcgtct ttgtactcgt aaatccaagt
    23461 ctgttggatg ccgcttgctg cagcttcgat gtcgtaaagg gaggttttgg cttgactgtg
    23521 cgatcgatag attgaatatc gcttaccagc gctggtttcc ccaaaaacag agcaagaata
    23581 ttgtttgcct ctacttcact cttgatcgag ggcttctcgc cgttgagcaa agctaattcg
    23641 gctcgcacaa tgtgctgatg ggccttaaga tgggcttcaa ttgtccagta ttcttcgggg
    23701 ataaaggatt ctacttcttt ttctcgatca cagatgagtt tgagagcaac cgactgtaca
    23761 cgaccggcag aaagtcctga ttttactttt ttccatagaa gcggtgaaag cgtatagcca
    23821 accagtctgt ctattatgcg cctggctttc tgagcttcca cgagattcat atctatatca
    23881 cgcggattag ccatcgcatc ctgtacagct tgctttgtta tctcattgaa agtaactctg
    23941 cttataggtg tatcttttgc tctatctttt aggtattttc ctatttgata cgcaatcgct
    24001 tctccttcac ggtctgggtc tgctgccaga atcaccgcgc tcgagcgctt tgcttcttcg
    24061 gttagttctt ttaatttctc ggcttttccg cgcacggtaa tgtattccgg ctcgaaatta
    24121 tgtgcaacat cgaccgcgat acgcgatttt ggaagatcga taacctggcc attggaggca
    24181 aggaccttgt accgagaacc aaggtatttc tcgattgtac gagcctttgc aggcgattcc
    24241 actattacaa gagtcttgag cttgtgagtt tcagccgcct tagcggcttc gggagtcttc
    24301 ttttccttgc ttttctttgc tgtcattcaa taacctcttg aaatgcaact ccttcgattg
    24361 cggcttgact tggtataaat catcgccgtc tatattccat tatattcgca ccggatcatg
    24421 ggaacgagtt cagcgccagc ttttattgcc aaagcgccat cttccttcag agcatccgct
    24481 cctgaatttc gagaagatcc aaggcaagag gcagcgaccg ccacatcccg accctctgac
    24541 agtgcaattt ccgcagtaat gagagctcca gatttctctg gagcctctac aactaatgtt
    24601 aatttagaaa atcctgcaag aatgcgattt cgttccggaa accgatatct atctagactt
    24661 acgcccggcg gatattccgt aaccaagcat cctccgcaat cgaggatttt cgctgccaaa
    24721 gctgcatttg aggagggata gactcgctct ggcccacagg gtaaaacagc gatagtagct
    24781 gcagcaccgc tttgaattgc tccacggtga gacgcggcat ctataccacg agcaattcca
    24841 gacactatca caataccttc atcagctgtt tcttttgcca gaagttgtgc agtataaagc
    24901 cctctcgatg tcggacttct tgtccccacc attgtcaccg atgcctgatt tagagccttt
    24961 gtctcgcctc gcacaaaaag accaaatggt gctctatgta tttcccttag aagcggtgga
    25021 taatctttat cgacaacgca gacaaatcta gctcctaatc gatctaatat gttgatatcc
    25081 ctctcggctt gttcccatat cttacccata tccaatggtc gtgcatgcat ggaacggtat
    25141 agaaagcttt cgatttcggc aagacccagg gctagaaaat cctccatgtc atgaatgttc
    25201 tcaagcagca aaatacgctc ctgccaactg agaaaatgaa gtctttggaa agcaagtctt
    25261 agagcaagag gagttttttc cattgaaggc tgaattgctg agatacgatc gagctctaat
    25321 tggatgagct tgcctattcc cttatgctgt ttcccgagtg tcatctgtga gaaccctcca
    25381 tgaggataag cgcatctaat ctccttctcc gctttcattt tcttgctttc ctgtttctat
    25441 gccttattct tctccgattt gcgtggaaaa tactccttgt gatacatcat cagcgattct
    25501 tcatcgatgt gagtatagat ctgcgttgta gctatatccg agtgccctag aagctcctga
    25561 acagtcctga gatctgctcc tcctgctaat aagtgggtgg caaaactatg tctaaatgta
    25621 tgcaccttgg cattgacccc tgatttttca cgtatcttgc tgaaacgctt ccagattccc
    25681 ttgcgagaga tgcctttgcc ctcctgatta agaaatacaa aatccgttct ctctcgtttt
    25741 tcgagaagag gccttgcctc tgccaaatag cgtgagagcc aatacttggc ttcgtctcca
    25801 aatggaacaa gacgttcttt tttccttttt cctatgactc ttagcagtct ttcttcgaga
    25861 tagagttgtt gaaatttaag attagctgcc tcggagatcc ttagtccgca agaatatatg
    25921 agttcgaaaa gcgccctatc cctaagccca ttaggtgaag taatgtcaat gcattccaat
    25981 acatgttcaa tggaatccgg atcgagcaca tcaggcagat tcctatcctg ctttggtgtg
    26041 cgtatttgca tcgatggatc gtctgctctg agtttttcgt tgagacaaaa gcgatagaat
    26101 gagtgcagac tagctataat acgagccatg gttgttcttt gaagccctga ctgtgacctg
    26161 ctgacgagat atgcaagaag atcttcttca ccagcatcga gcggcgtttt tccatattga
    26221 aaaaggaaat tctccagcat ttctatctca cgaagatatg catccgcagt aagtacggag
    26281 cgtctccttg tagcgagtag ataggcttga aaacgccgga gtagaatact caccttctgc
    26341 ctccgaagtc ttcgggcatt aagcgtttat tgcgaagtgc tgttgggatg tcgaattcgt
    26401 cattatcgga ttccggatcg aaacttattc tgccaaaatt atccttaaaa gaggatttat
    26461 cctgattctc gcttgcttga tctcgctttt gggctggttt ttcttcatgc aggggccatt
    26521 cgcctgggct aagaaattcg cttggacgaa ttgatcctaa aggcatcgca atggtgttct
    26581 ttgttacagt cttttctcct agtttttgat gcaaggcacc aaaaccagta gcgatcaccg
    26641 tcactctcac ctcatctttt atagaactat ctgttactac accgacaatg atgtgggcat
    26701 cttcggccgc ctttgatgtt attagatcga tgacttcctg atattctatt agagaaaagt
    26761 cctcgccaca tgtgacattt acaaggatat ttcgcgcgcc ttcgatgcat gcatcatcga
    26821 gaagagggtt atttatcgct ttctccgatg cttcttgtgc gcgattgtct ccgctgccga
    26881 cgccgatacc cattattgca tcgccctgcc cttccattac ggtccggaca tcagcaaaat
    26941 ctatattgat atctccagca agtgttatca ggtcagagat tccctgcacc ccctgtctga
    27001 gtacttcatc agcaattcga aatgcttctt ttaacggggt tttcttgtct acaaccctaa
    27061 gaagattctg attgggtatg acgattaccg tatcggcagc ctgttttagc ttcgaaaaac
    27121 cttcttctgc tattctgttt attattttgc cttcaaaagc gaaaggtttt gtaacgactg
    27181 caaccgtcaa tgctccaaga tctcgggctg tctgtgcaat tatcggagca gaacccgtac
    27241 ccgtgcctcc accaagacca gctgttacaa agaccatgtc ggctccgcgg agcgcttgct
    27301 cgatcttatc tctgtcttct atggcagcct tctctccaat ctctggtttt cctccggcgc
    27361 cgagcccttt tgttattttt gatcctattc caagccttat ttccgctttt gcacgaccta
    27421 gatcctgcaa atcggtgttt accgcaataa attgcacatt cttcaaccct gccataatca
    27481 tgcgatttac tgcgttcgag cctccgccac cagttccaat gacctttatc acagttgggc
    27541 ttcctatacc attgtcgtca acaagttcta ttgccatatt cttcctccta tacaagcatc
    27601 agaaaaacct atcttttatc cattttatgg gagaaaatcc accttctttg ccttttcgtg
    27661 cttttcttct cgtcgaaggc tccggtgttc caattcgttc tgcctcaagc aaaactatgc
    27721 caacagccgc agagaatgcc ggtgaacgaa aacccacatc aaggccgggc aaggctgtag
    27781 gtgctcctat tcttacaggc accccgaata ctgaccgcgc acattcgtct atatgaggca
    27841 gtagagctga gccccctgta agaacgactc cagcctttat cagttctttc gatccatttt
    27901 ttaccagtcg ttcatttacc atttcgaata tttcttctac acgtggctgc acgatcgata
    27961 ccagttttct gcgttctaat tccactggtt cgcgtgctcc gaaccccggc acaatgacag
    28021 cctcatccgg atcgatagcc tcggtccatg cggctccaga ttcttttttt aggcgctcag
    28081 cgctatcgat gggaatactg agcattatcg atatatcgcc cgttatttgg cttcctccca
    28141 agggaatggc cgatgtatag acgggatcgc tttccgaaaa aaccatcaga tctgtagtgc
    28201 ctcctcctat atcgaggaga agacagccta actcacgttc atcctctgtg agcactgtgc
    28261 gcgcacagcc caatattccg agatagctct gttcaacctt gtaacctgcc cttgtaacgc
    28321 atttcaacat attttgtacg gttgataccg agcccgttat gatatggaca ctgcattcaa
    28381 gccgaacacc tgtcatgtgg agcggattac gaattccctt ttgatcgtcc accgtatagg
    28441 atcgagggac gacatgaaga atctcgcgat ccattgaaat tgagaccgca cgagcagcct
    28501 catgaacgcg agcgatatcc tctgccgtta tttcgcggcc tcgccctgtt accgctatct
    28561 gtccattggt attgaaactc tcgatgttcg caccggaaag agccagcgag acttgcctaa
    28621 tatctattcc cgacataagc tccgctgcat caatggcagc gttgatacta ttcaaagttc
    28681 cttcgatatt gagcactact ccgcgtcgca gcccactcga aggtgccgct ccaatgccaa
    28741 agatttcaaa ttgtcccatc tcgttgaatc caccgacaat cgcttttgta acagtggttc
    28801 caatatccag aactacaata cttgtatcag ccagagactc cctcctttgc ctgatagatt
    28861 acggtgccgg aatgaaaatc tatttcgctt ggctggtcac ttaaaccgcg cgcatgcaaa
    28921 atatcaagaa ccaccaatgc attgcataga gcttcggcac tgaatctgag cggtaagcgt
    28981 accgccgtct tgacatgcat gggataaagc acaatttcga tattgtttgc cgagattctt
    29041 tccacatgta tttctgaaaa cgcccttagt agttcgggtg aatcttgctt gataaccgct
    29101 aaatttgcaa aaagcggcag tatggcttcc ggtagctgct ggcccaaact gaactgctca
    29161 aatctaatgc ctgataccac cggtagctcg ggattattgg aatcgtctgc gtcaagagaa
    29221 gcaaatgcta ttccctcgcc atcgaccaga atgagttttg aaccttgttc ggtgttaatc
    29281 gttatcgatg ctaccgccag acgctctcgg atatcgatcg ctagtgtgga aggcaatacc
    29341 ctatagactt tcgcgccagc tattctaggg tttttcataa tcgaacttgc tatctcttct
    29401 atatctatcg aaagaatatt ttcatctgca atctggccta aggactcgat tattgagttt
    29461 tctgatatag tctctaagcc gcttacccta actgcatcga tcttggtaag tcgtggcagc
    29521 gatatagccg ctccagccga tagaacacat aataaggtaa ttgcaattag gatatgtaag
    29581 cttctatgtc tcttcgaagc catcgatgtc ttggcaggct ttacggactc actgagtttt
    29641 tcggcttgta cagatagggc aacagaatct tgtggagcac gccttatgaa tggattaacg
    29701 gaaatctttg tcgcggatag ggattgggct ttctcttctg ttgctcgatg agcttgttct
    29761 gaggaggtat ttattctctg tgatatttgg atcttctcgc ccgcatttat tctggtcccc
    29821 gagcttttcg ccgttgaact gccagggagg gcagtctcac agcgtgtagt ggcctggatt
    29881 atatctatag aataattatc agatttcgag agaagcgaat gcgttccctt ttttctcagg
    29941 gcttttgtct cattgccctg ttcctgcaat ctgacatcag tcatatgccg ccccttcttt
    30001 tcttaaggct gctctcatgc ctgtctcagt ttgaccactt cgagagagat tgatgagaat
    30061 gccacaagaa attgcgctca taagtagtgc cgagccacca gctgaaaaga aaggaagggc
    30121 tagaccggta gttggcatca ctccagtaac aacagcaaga ttcagaagaa cctgcaaaga
    30181 taagagagtt atcattccaa agcctgcaag cctagaaaag taatctgatc caataagtat
    30241 tctgcccgcc cgtatcagta tgaaggacca aagccccata accaccaaga cacccgcaaa
    30301 tccagattct tccacaaatg ccgcaaatat aaagtcagat tgaacttctg gaatggagct
    30361 gatttttagt gtgccaagtc cgacaccctt cccccagacg ccaccggaac gaatcgctct
    30421 gagtgcgcta tacatctgat aattcactgt ttgcgtgtgc tgttctggca tgagaaaacc
    30481 aaccaggcgt tgtactctat actgcgagga aaagatcata agagcaccat agagaatcaa
    30541 aaataatgct aggttgctca tgaatagaaa tggagtacct gcgagataca gcattgcaaa
    30601 tgcaatggcg agtactagaa tagaagtcga aaaatcctgt tgaagaaaga tcaggactgc
    30661 ggatatgatc acaaggacaa tcgcaggtaa agcaaccaga cccgattcga caagtcgctc
    30721 tttccgtttt tctagaaaag atgcgagata caacaccatt accggtttcc agatttccga
    30781 aggctgaaga ctgaaagaac cgatccggat ccaacggcgt gccctattga ttgtcatccc
    30841 cagagggctg aatattggca aagaaagacc aattacgcat aagacgactg caacaggcaa
    30901 gaccgcacgc aggagcttca taggaataac cgctgctacg gccatacaaa tgagcgatgc
    30961 cgcatagaga atagcttgtt tgattattac cgagttaggt cccccattgc gtatggcaaa
    31021 accgattgag ccagaccaca gggtcattat tcctatcaat gagactataa ttattgcgca
    31081 gaccatttgc caatctattc gatgtgagtc tatacgcaag ctcttgcttt tgctctcttc
    31141 tttcatctac caacaacctc attgaatctt cagcgttgac aatgcaatca aggcaaaaat
    31201 acctcccata atccaaaacc tcacgacaac ttttgtctcc ttcaaaccac ttagctcgaa
    31261 gtgatggtga agaggtgcca tgaggaaaag ccttttcccc ttggtcagct tgaagtagac
    31321 cacttgcagc attacggagc caatctcgac cacaaaaaca ccaccaatga ccaaaagaag
    31381 aatttctttt ttaaccatga gcgagagtac cgccaggact ccgccaagag agagagaacc
    31441 tacatctccc atgaatatct ctgctggatg agcattaaac cataggaatc ctacgcaggc
    31501 acccaacaaa gcaaaattga acaccgtgag ttcgctcgcc tgcttgatat atggaagacc
    31561 cagatattgc gaccaatctg ctcggctagt tacgtatgtc aggacagaaa aagccaggac
    31621 tgcaaagatt acgagcccca cggcaagacc atctagccca tcggttatgt tgaccgcatt
    31681 cgaccatgtg gcaacataaa taatggctat tggcaaccaa tatactccga gatctacgac
    31741 atggaccttg aaaaatggaa catataactt tgttatagaa ggaccggttg tgcgataaat
    31801 cgaatagaca attatagtcg agacaatgaa ttgtaggatt agcttttgaa caacagagag
    31861 tccatccgag ctatgtttct taatcttgag gatgtcatcc gcagctccga ttaggccaaa
    31921 gcctataagc gccgaaagtg cgatccaaca atatacgctt gatatatcta tccataacac
    31981 aacagatatc aagaccgaca atattatcaa aataccaccc atagtgggtg ttcctgtttt
    32041 cgccaaatga ctctgaggtc cgtctaatcg caccgattgc ccaaacttga gcacccttaa
    32101 ccgatcgata agccacgggc ccaagacata gctaatcaaa agagcagtga ctgcagcgta
    32161 agcggatcgg aaagtcagat atctgaaaat attgaaaggg gtaaaatatt ttactaaagg
    32221 aagaagaaac tcacgaagca tgacttcctc ctttgctcgc ttcagacaaa tctatgagcc
    32281 ctttttcctg gaaagcgtgc gagatccttt caagggccat acttcgcgaa ccctttatga
    32341 gcacaagatc accttctcta acataactgg aaaccgtatc aatcagattc cccatgtctg
    32401 tcgtatgcat caagacgcct ctaaaagcag cttctttgag tgcggcttcg gctgctttca
    32461 tttcttctcc aaaaagaaaa acagcctctg gcttcatttc tgccaccata aaacccacat
    32521 cgcgatgcgc attatcgctt tcttttccaa gctccaacat ggaaccgagg atcaagactt
    32581 tgcgattcgt cgatgatatt ccagaaagga agtcgatcgc ggcagccatg ctgttggggt
    32641 ttgcattgta ataatcctga aatacagtga tcttgccttt acagatttcc gagcgaccaa
    32701 aaagaggctg cacttttgaa agcccctgaa ccgctaaaat cggatcaagc tccataagta
    32761 tcgctatcga taaggcagca catgcattta ggagattgtg tttgccggga agacggaaat
    32821 ctaaggtctg acctttccat tcgattttcc aaccatccag acctttgttc tcgatgcgcc
    32881 caagtccctc agtactacga agcccaaaat aataaacaga acctttcact tttgaagaaa
    32941 ggtaggttct gtagtcgtcg tcttcccata acaaggcctt ttgttggcca tcgaaatatg
    33001 agaatatccc gcttttttcc cttgcaattt cgtctctaga accaaacatg ccaacatgtg
    33061 ccgagcctat attggttatg agcgcaattt ccggctcgta cattttggct agctcgtcca
    33121 tctcaccctt gcgatttatg cccatctcaa agatgcctat cttgtgtcga ggttcaatgc
    33181 caaaaatggc aagagcaagc ccaatatcag aattcagatt tccaggactc attgcgatgc
    33241 tctcttctgg gaagcatgct gatagcatag aagcaatgca ttccttggta gttgtcttac
    33301 cgctagatcc tgtcactcca attctgagta gattctgcct tcttcgatat tcccgagccg
    33361 cattctgaag gccagaaaga ggagaatcaa taaggagaag acaggtttcc gcaacaagac
    33421 cgctatctcg aattgaagca atagccgcat ctttttcatc tatttccgca agaattgtcc
    33481 ttgctccatt tttgattgcc tcgagaatat gatgatgacc atctgttctc tcgcccttga
    33541 gagcaacaaa catgctgttt ggctctacta gcctcgaatc ggtgacaacg gaacatatcg
    33601 gagtattaag ctcgccctta caagtggctc caatgatacc agcaagttcg cccgcgtaga
    33661 acaaaacctg ctggtcggaa gcgatgctat tgtcttccat tattgctcac ctccgaagcc
    33721 ttctgttcct gtctctcggt tacgatgata cgctgttccg gagtcgcgac tatcattcct
    33781 tttctgattg cggctctctc aatacgctcc aggttggcaa gttttgtgat atctgcatcc
    33841 aattctctgc ccacttttag cagatccagc tcagcccgct ctaattctct tacttcggct
    33901 ctcaaagcag catagcgcgt tgtttgatac accagggcta taaacaaaag gcagattatt
    33961 attccggaaa accaaaagcg ccttgcattc atttctgttc ctctcgagca tttgctcgaa
    34021 tggctcgaaa tttagcggat cgtgatgagg gattatttcg tagctcttct aatgttggag
    34081 actggggctt tttccattcg agcgaaaagt ggctattctt ggcttcttcc gcgaatatgt
    34141 tcttgacaat gcgatcttct agtgaatgaa aagttatcac cgcacagacg gctccggggc
    34201 tgagcaaatt cagagcatcg atacaagctc tctcaagacg accaagctca tcgttgacca
    34261 agatgcgaag cgcttgaaat gttcgggttg ccggatgtat gcgtccgttg cgatatttga
    34321 caggcactgc accagatatg atcgaagcca actcagatga tgtacggata cgttctttct
    34381 tcctcttttg aactatcgca tgcgcaattt gtctggaaaa gcgttcttct ccaaatttga
    34441 aaattgcatc cgccagttcc atttccgaaa gtcggttgac cagttctcct gcggtacact
    34501 gtgaatatgg ataaagacgc atgtccaatg gctcatctgc atagaaggaa aaacctcttc
    34561 ctgagctcct aagctgatac atcgagatac caagatcgaa tagtacgaga gaaaatcttg
    34621 attcgccatt ctcattgcaa agacaggaag aaaagtcacc gggaatgcaa taaccagctc
    34681 tcttatttcg aatgtcagaa agaacctcat catagtaacc attgattatt aaaagcctat
    34741 ctgcaaacgg cttaagtcgc tcggcagcac gattgcaagc atctggatct gcgtcgatgc
    34801 caatgcaacg aagcccttca tattttttta agagcgcttc ggcatgtccg cccgctccaa
    34861 ttgtgcaatc tacatagagg gtcatgggct ttattgtgga ggcaagattg catagctgtt
    34921 ccagcattac tggcgtatgt aagaaaaaat ccatgccgct ctctttatca gagtagggtt
    34981 tttgcgttcg tatcatgcgc ttaccctgca tagacaatcc ctcaagaaag tgtaagccca
    35041 ccaagctctt cggcagccat aaggaactcc gattgttttt ctgccatata agcttgatat
    35101 ctctgacagt cccatatttc gatataacga gaaatggcaa ggataacaca ctctttcgat
    35161 aatccagccc attctctcag gctctgagga atgaatattc gacccagttt gtccacctct
    35221 acttcttggg caggagcaat gattcttcgc tgtattaggc gcgaacgggc ttgaaaaaga
    35281 cttgtggtgg caagaatccg ctgagaaaga acttcccatt cttcaggcac aaagagccat
    35341 aagcaagggt ctactccctg cgtgagtacg agcgtcgacg atagattagc tcgcaagcgg
    35401 gcaggaagag aaagacggcc tttctcatca agagtgtttc tgaattcacc ggtcaaagat
    35461 ttcattccca ctatttccta ccatttacca caatttacca cctatttatc attttaagcg
    35521 ctaatcttga tgtgtcaacg aaactttagg aaaaagaatc gacagaaaac aaaaacctat
    35581 cttttttggc tatacatatg tatagggtca tgagatttgg cagtatggaa attttcagtc
    35641 aagcagagta tcgaccaacg aggcaatctt gctggaatcg atgccatcta tgggccggat
    35701 atatattctg attgttctca acgcttcagc tactttttcc actatattgg gacaaaatag
    35761 agttgaacgc tgatcaaaag gttcgaaaga attgtccaca tgaaccatca aatcggtttc
    35821 taacttgatc cgctcaggaa tatctataac tacatcattt tctgctatat ccgccgcttg
    35881 agcaagcgcg ctctccgctt ccgtgcgctt tacgatatcc aataggtctc tatgcagagg
    35941 gttggattca tcgaaaacat gttcagccgc aagcttgtat actcgtttct cttcagcttc
    36001 ccttgcaagc ctaaaaggag ggaatctagg actactcata agccgattga aattcgtatc
    36061 atcgatacca tacagttgcg aaggtataag gacattagaa gccaatccca ggtacactgc
    36121 ctttctgacc atggctgttg cggctcttac aacggagtgc cagtaaacag cgcgatacat
    36181 ttgatacttt gcaaagatca gggcttcaat gctcatttca cctttcaaat cgatggcaag
    36241 tctgtcatca tgcactatga gtcgtcgcat tatgtagtca gcatcttgta tgccatatgg
    36301 aacgccacaa tagaatgcat cacgcgtcaa gtaatcgatt ttatctggat ctagtacacc
    36361 tgataaaaaa gagcagaaaa gaagcgtctc tctgctctta ttctctgttc ggtcgggatc
    36421 tatgatagat gcggtctgct cagggtccgc gccacattca agaatgtgct ctcttagagg
    36481 acttgaaatt atcatttctc cagcaagagc ttcatggcgc tcaaggggaa gttcttttag
    36541 agaatgcgca tagggataat gcccgatatc atggcaaaga gatgcaacca aaaaagagcg
    36601 aagcccttcc ttgctgacaa agtctatctc tcccttttca actaaagcca aggccatacg
    36661 ccgcgctata tgatatactc cgaggctatg agctttcctg gtatgcgttg cgccgggata
    36721 aaccagtgct actggtccca gctgtcggat tcggtctaaa accatgaagg ctctggattg
    36781 aaatagcgca tggagcgatg aatccatgtg cacatctccc catacaggat ctcttaccac
    36841 aacatcgaag ccatgcaaga gtgcattcaa gagcgaaatt tccgacatgt ccgaatcagc
    36901 ctctttttca aagagttttc aaatggtctt tatgtctatg aatcgacgat tcaattctac
    36961 cagccaccta tcagatcatg caactcagtt gactggtcgt aaaaaaatag tctgagcgcc
    37021 gactccccat aatgctacca aaatgctgac tattatgccc gccagaatga cacctccggc
    37081 aatggcgaaa aaagccttct tatgcgaaag cccaagtatc catgcaccca aagtccctgt
    37141 ccatgcaccc gttgctggca ggggtatagc cacaaagagc aagagacccc aatagccata
    37201 tttttccact tttgtgtgta ccttagctcg cgctctattc acaactctgt cgaagaatcg
    37261 cttatattgc ggaatcttat ataagagtct atgaaggctt tccaggaata aaaaagctat
    37321 aattggtata catatattag cggcaataga tatgatagct gcaataggaa tggaaaatcc
    37381 cttgtaaacg gcgtatggaa ttgctcccct caattccgac actggaagaa tcgaaagaac
    37441 aatcgtccta atgaggtctg cattcatcac gggcactata gcgacctcgc ttcagtctgt
    37501 ctatagaaag agcaaataga tgaaaactag ggctatgtta cgccatcttg gaatgccaaa
    37561 aatcgcagaa cgtcactgtg catagcctcg ctttagctat tcgatccgat ttctttcaaa
    37621 actcgataaa aatctgcagg atatggctct atccagataa ttcctggcaa agcgcggaaa
    37681 aatgtcattt gacgctttgc gtattgcctt gtgtgcaatt ctatggcact ggatattctt
    37741 tcgatcggat cctcttcgag ttcgaaaaat tcgcgatatc caatggctcg aagacccgga
    37801 tccttagaac tatagcccag actcttaagt ttctcaattt cggaagcaag accttccttc
    37861 atcatggctt caactcgttc atggattcgt acatgcaatt cctcacgact tcgcgttatt
    37921 ccaataacaa tgaattttct acctgaacga atggccttct taggggcaaa tagacttggc
    37981 ggttttcctg aggtcctgag aatctcgatg gctcttgtaa gtctatagat atctcgctca
    38041 tgaattcgag cggcggcatt gggatcaacc tccgcaagct cttctcttaa gacttgaggc
    38101 ccgaattcat ccagatctct accaacctgg gcacgaattg ctcgggagct tggcggagaa
    38161 gtgggcaagc cacagatgaa attcatgagg taatagccag ttccaccact caaaataggc
    38221 aaaatccctt gagctgaaag ctgattgcac aatgcatcgg ctcgctcgac aaactcgcct
    38281 gcagtgtact gctcatcggg attttttatg tctataagat gatgaggaag tctcttgagt
    38341 aaatccttcg aaggcttagc cgttccaata tccagaaagc gataggcttg catagaatcc
    38401 gcggagatga cttgagcagc atttagatca gcgtgccata ggcttcgaag tcgtggaatc
    38461 cataaatctg cgccttcacc aaaaatagtg tctaaaagat gggttttccc agatgcag
//
LOCUS       DAOOWY010000007        36865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1165_length_36865_cov_9.964928, whole genome shotgun sequence.
ACCESSION   DAOOWY010000007 DAOOWY010000000
VERSION     DAOOWY010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 36865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36865
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1165_length_36865_cov_9.964928"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..2464
                     /gene="thrA"
                     /locus_tag="PK105_01235"
     CDS             26..2464
                     /gene="thrA"
                     /locus_tag="PK105_01235"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOU60357.1"
                     /translation="MKILKFGGTSTGSPEALKSLVRIVRQSGEKILVVSALSGTTDAL
                     LCSAKLASEGGDWQSVFASIRERHYSMISALLQPPYKEIATGETRELLDELSSLLLGI
                     SLVRDLSPRTLDLVASYGERLSAGIIAETFSQTGLIAEAVDARKFIITDDKFGAAHWL
                     ESETIPRVESFFSDFEHIAVVTGFIASTIEGITTTLGRGGSDFTASILAACLEAEEIQ
                     IWTDVDGIMTADPRAVPDAFVIPEISYLEAMEMSHFGAKVLHPPAILPAMDRGIPVRI
                     LNTFNPDKPGTRIVRQGAPSSWPVRGIASIPSISLLLLQGPGLPGVAGIAARMFAALA
                     SAGINIILITQGSSELSICCAVLPQDSGKGVLALKEEFKYEIGAGTIQNPSIEQDLSI
                     IAVVGEMMKHRVGISGRVFKALGRNGINVVAIAQGSSELNISIAISVQDRNKAMCSIH
                     DAFFLAGVRTVNVFLVGTGLIGSTLLAQVAAQHRRLFRDRSIRLRIAGIANSRQMLID
                     PRGIDLENWKDALQSGKKTDISAFIQEILSLNLPNACFCDCTASEELAQHYEKLLSSS
                     ISVVTPNKRANSGPLREYQRLMDIAREMDVPYRYETTVGAGLPVIGTIQDLIASGDEI
                     LRIEAVLSGTMSFIFNNLGEKHSFSSLVAEAKLRGFTEPDPRDDLSAKDIVRKMIILA
                     REAGFPLEESQIQIESLLPKRIIDAPTVEEFLALLPEMDQMMKKCYDSASRSGAVLRY
                     VAIVTSRDARLSLSSFGPASPFYSLSGTDNMVVITSKRYSQNPLVIRGPGAGAEVTAG
                     GVFADILKTAESYL"
     gene            complement(2573..4567)
                     /locus_tag="PK105_01240"
     CDS             complement(2573..4567)
                     /locus_tag="PK105_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HOU60358.1"
                     /translation="MSDLPIHEQETSTDAQEILKKFDKESNFRIYSGFFAKFISALAI
                     AFSVFQLYTAIFGVLDAMLQRSVHLSFGLALIYLLYPASKKWSFTKLHPLDALLAVAG
                     ALAPMYIIINYQKLVLRAGTATPMDIVFGIIGILLVLEAARRVVGIPMVVIAVVFIIY
                     AFIGPYIPGKLAHRGANFETLIQHLYFTTEGIFGIPLGVSSTFIFLFILFGAFLERTG
                     LGQLFIDLANSVAGWAAGGPAKVAVISSALLGTVSGSSVANVVGTGSFTIPMMKRLGY
                     KPEFAGAVEATASTGGQLMPPIMGAAAFLMAEFTGIPYSRIIGAAVVPAILYYFGVWA
                     GVHFEAKKNGLRGLSREELPKLKQVITERGHLIIPLIAIIYLLVSGYTPMRAALWAIV
                     LSIISSWIKKGTRIPPIEIVRALEAGARAALGVLAATACAGIIIGIVTLTGLGLKLGS
                     VLVDLAGGMLIPTLFFTMLTSLILGMGVPTTANYVITSTITAPAVIMLLSRKAGLDPY
                     AVAPANIILPAHMFAFYFGIIADVTPPVALAAFAGAGIAKANPMKTGINASKLAIAAF
                     LVPYIFVMNPQMLLFDVNLFSFVWMLITSLVGIISVASAVNGWLMTNTVWYERIIGFV
                     GGVLLIDPGIKTDIIGIGLIALLSISQFMRSKKLKAKASS"
     gene            complement(4564..5040)
                     /locus_tag="PK105_01245"
     CDS             complement(4564..5040)
                     /locus_tag="PK105_01245"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HOU60359.1"
                     /translation="MLLGCLILIIALSAIAMNLRVVPVLIIKNGEDKVVARLPLHDGQ
                     FIHHYIHSIHKTAVDEYFHIKGRNLELFQVKYDTYGVGMPSDGGEAFAIEDGRFVVKM
                     HRTFFDIPILVSIVPQHGVIVEDELFLFTQWVLPESRITLSAGRMLIFYNGRSSPK"
     gene            complement(5167..6108)
                     /locus_tag="PK105_01250"
     CDS             complement(5167..6108)
                     /locus_tag="PK105_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOU60360.1"
                     /translation="MKRFLVLGICLLMATSAFAQQQFITIATGGTAGTYYPLGGALAD
                     IWNKNISKMNATAQATGASIANINLLKGGNVDVIFTQNDVASYSYNGTELLKDQAYKD
                     LRGMACLYNETIQLVALESSGIKTIGDLKGKKVSVGAAGSGTEANARQILGAAGLTYN
                     DIKVQYLSFGESANNMKDGNIDAAFNTAGIPTAAIQDLAVSKKMVLVPIDGQIAAKLM
                     AQYPFYALQVVPAGTYQGQDQAVTTVAVKSMLAVSSKLSSDLVYKMLETMYANTDRLI
                     AAHSQGKNVKIESALEGMSIPLHAGAEKFFKEKGALK"
     gene            6504..7586
                     /locus_tag="PK105_01255"
     CDS             6504..7586
                     /locus_tag="PK105_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60361.1"
                     /translation="MASKDSAFFIFSEGISMREEFQRYGEQGSVFLLEFSSIDSKIDA
                     VKIRFLDNSADEWNGLGPRPSHSLKGNINLKQGLHTAAFAIDGRFLLCESSGKRLFSF
                     KDGAIELLLELPAAVYLGYAKNPIEACRLWHIWTMPDRHLARWIEVHKELEPQEDIGS
                     LKPGNAAGLLGSLSFSGARPNDFQELNLSIAAKAEGREDWKIADLMLCQPDIALPLLF
                     RREIPMNLELGNNRKRAQNRYAKIRAGLAQYWQFCEKRWTNEGIPALFHPSILFPDDC
                     NNAESDNMLFSGPDLVFAPNMASKDEFCSLILPKGSWIHLWTSRIYPAGKVTVYAPFG
                     MPALFYRLESEYAWLFDSVRQRASRL"
     gene            7590..8390
                     /locus_tag="PK105_01260"
     CDS             7590..8390
                     /locus_tag="PK105_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60362.1"
                     /translation="MNTITAYFAALFGNFLLSLGFSLQKKHVHWLSAKRRGESIRRGE
                     IIGWASGFVLMNVQPIFNYIALETLAPNIVAALGGSNIIFTIVLSYFLLGERMPPRKI
                     PFFVLLMASLVYAGVVGQEASRQFNVQAFWVAFFIPTAFSMLVLFASKRMSEDRIGLF
                     LGSSAGALGGFMVLALEALRINYGSNILSWFVSAYLYVYFFCGIASFFIKQFAFERGK
                     MTAVAPSFYGLLVLYPSIASYFVSNMPVHASQLIAFGGISVAIVLISI"
     gene            complement(8493..9728)
                     /gene="ispF"
                     /locus_tag="PK105_01265"
     CDS             complement(8493..9728)
                     /gene="ispF"
                     /locus_tag="PK105_01265"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOU60363.1"
                     /translation="MSSSVQKTISVHRLPIEGPSFGLITAAGSSSRYGSSKKELEDIG
                     GFTVLERAVEPFIPFCNAILITCPQGEKSEFEELFSKSKLKPDLASLQAGFLITEGGS
                     SRQESVLFGLRELAAHCNNDGVVLIHDGARPWISKKLIEKTLCCARQRGACLPLMPLT
                     ETPKVVEDDLIHDHPARGIIMCAQTPQAFSLMEILAAHELAHLEGFRATDDSMIWDHY
                     VGPVSWIEGERKNRKITFKEDLMSGLLAHESLSFPMPLRIGLGYDIHPLVDGRPLLLA
                     GVRIESEKGEAGYSDGDVLWHAIIDAMLGAAAAGDIGLLFPPGEPEWKDADSSVLARK
                     AFMLIRSKGWQLSNIDCTIIIEKPRLAPYREAIKASIAANLELSMESISLKAKTKEGF
                     DATGRGEAIEAHAVVMICR"
     gene            complement(9725..10225)
                     /locus_tag="PK105_01270"
     CDS             complement(9725..10225)
                     /locus_tag="PK105_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOU60364.1"
                     /translation="MPNDQTTTFLVDQRVVYPSQGVGRIMEIREKTFNNQKTLYYIIY
                     LEFSDMTVMVPVDKVEVLGIRPLVSRKEAEVALDSISKAASTIPSDWKLRYQMNLDLL
                     KKGSITDIAAVVRSLYQRSKIKELPILERKLYESALNLLQDEIAFALDRPKEEIAAII
                     RENLEK"
     assembly_gap    10361..10386
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10487..12682)
                     /gene="recJ"
                     /locus_tag="PK105_01275"
     CDS             complement(10487..12682)
                     /gene="recJ"
                     /locus_tag="PK105_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOU60365.1"
                     /translation="MKWIKREIDPVQVRGLSQRYEVDTLTASILVRRNITDPEQIQFY
                     LENDLQLLHNPFLFNDMEDAVDRIITAREEEEKVLVFGDSDADGITSTVLMTEALRDF
                     GLEVFQKVPEGEEPYGLSKSTIDFAKEQGVSLIVTVDCGISNHEEVLYASQEGIDVII
                     TDHHRIQTEEPPKAIAILDPKLPDCGYPFRDLSGCGVALKLAHALAIARLGICKEPLA
                     LLRAGQAIHQEICPGAGASKLNTEKEANLEIEAIRLDNLLEASRLKITSDNRGYFPSD
                     TLEKLEKFLRGRIVIVWNKNTTEAFFKKNFAGLVELEVIDLEKLAATVWPNMSGNSLE
                     GLVRLSRLKKYAHGAFGAIDALKHIFNAYLLHTLQSQSLLNDRMFQLTAFGTIADLMP
                     LKNENRLIVRRGIESLNTAPTNGIRELKMALNLGKPLGSNDVAWQITPIINAAGRLGR
                     PKLALDLFQAKTAIEATETALQLIQANNERRRLGTEAWGLIQDQLAESVKTSGGKFAI
                     VGSSRIKSGITGLLASRAANIMKIPVIVAVFKTDGICTGSIRGGDAFPLSQILAYCAD
                     LFLDYGGHDSAAGFTMKTDNWQAFLERLAQFMARTELITEEPEFLIDAELPHSFVTPD
                     LLQLCRYFEPIGEESDPLVFCSRNVSMVDAQIVGKNGKSHLKLTLDFGRYKWPAMLWD
                     GAKRLERDFSFRDNDKVDIIYKVTTNYWNGEERPQLELYDVHRSESPSL"
     gene            complement(12679..13989)
                     /locus_tag="PK105_01280"
     CDS             complement(12679..13989)
                     /locus_tag="PK105_01280"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOU60366.1"
                     /translation="MRAVDIIIKKRHGESLDEKEIRFFIEGYVNGEIPDYQVSALLMA
                     IFFQGMSAQETAALTQAMLDSGERINLCGIPGPFIDKHSTGGVGDKVSLPLAPMVAAC
                     GIKVPMMSGRALGHTGGTLDKLESIPGYMTSLEPAQFRKGLLEEGFAMTGQTAHIVPA
                     DKKLYALRDVTGTIESIPLITASILSKKVAEGAEGIVFDVKCGSGAFMKSYDDAKALA
                     KSLVATANTMGKRVVAVLTDMSQPLGFKVGNFLEIEETLDCLEGKGPKDVMELIYWLG
                     AWMLLLGGKAKDISECQNLCQEAIESKAALKLFYRNVVRQGGDLEEMQKRRGSWRSEF
                     SMEIRAEKDGYIVSIDALRIGLAGVYLGVGRNRTEDCVSPTAGFIFDKKSGDPVTKGE
                     RIATLFGKDSQSLEDAFPIALDAISIGDSAPSASSIILEEITVS"
     gene            complement(14050..14847)
                     /locus_tag="PK105_01285"
     CDS             complement(14050..14847)
                     /locus_tag="PK105_01285"
                     /inference="COORDINATES: protein motif:HMM:NF023393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HOU60367.1"
                     /translation="MAQRNERYRGLFLHTKDTARGDRILTFLCNEGLLSLFMFGGPKN
                     SLRSAALPFVLADIEVYRDSRRDFIKLTGANVLEVFDGIRKSFFHMQSASAASEFLIR
                     TSAFGGEYQQALLMITLLLRELARLDESETRLVLSAFFWDALYPLGLIPDLLRCERCG
                     RLFSEKNSLPGGLLVGESSFLCRSCAEEYGQIRFSTSVMLPFDDKILTCLHSIPRQSY
                     GQAVLLLKSIPSSPDIIKIIESLAEAAAEGPLQSLRILDQTFYRTQI"
     gene            complement(14851..15579)
                     /locus_tag="PK105_01290"
     CDS             complement(14851..15579)
                     /locus_tag="PK105_01290"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU60368.1"
                     /translation="MIPIGDDNTCRRGSAIVNWILIALNILVFVFLQKFGLDDYTTLA
                     LAAIPEEILSGRRLFTLITSQFTHAGFAHLIGNMMFLGIFGDNVECRIGKLRYILLYL
                     ISGTIGMLLQIFLAAFAKGAALQMPLVGASAAISGVLATYLVLFPGNRVVVLLFYFIP
                     TVFSAWFVIGTWFFMQIIGGLSGLTAIQNGGTAYFAHIGGFLSAYIWGKSYKKKESKR
                     IAEWRKGPTAGYSDGFHWWIVDDD"
     gene            15673..16341
                     /locus_tag="PK105_01295"
     CDS             15673..16341
                     /locus_tag="PK105_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOU60369.1"
                     /translation="MIQNKHMICSLLFDLDNTLYSEATGLENGVAQRINLFIADFFGL
                     SISEAIRVRREHAKAYGTTLEWLMRDYGFQEPEEYFRAIHPEDEADCLNPDMVLKLIL
                     NNIPLPKAVLTNAPMDHAIRILDKLGISECFSGIYDIWFNKLVGKPHPGSYLGVLEAS
                     GFTLEHTLFIDDLPRYVKGYIDIGGLAVLKDEMDRFSDLPFRRLKTIYELDQILDQLN
                     SSYI"
     assembly_gap    16351..16450
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16473..17360)
                     /locus_tag="PK105_01300"
     CDS             complement(16473..17360)
                     /locus_tag="PK105_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60370.1"
                     /translation="MNLSLMALIDFLRYTIAAATPLLFASIGGLFSELSGMLNIALEG
                     LMAIGAFFGMAAAGLTNNIFLGALAAILSASLASMLMGLMTSKFRTNIFITGLAINML
                     AQGITAVLSQAWYHTKAVVAFDIPIDSHLFSSSISQLPLIGVLFSYRSMFTICAWLWV
                     LISAFIIKNTRHGLRIRATGSNPQAVSALGFSPDRIHMQALIWSGIGSGLGGYALGLS
                     ISAFVPNIISGRGWIALVAIYLGRKRPFWVAASCLLFAFAESSSNYLQGFSAIPPLLV
                     LALPYLVTLISLILAKREQ"
     gene            complement(17360..18394)
                     /locus_tag="PK105_01305"
     CDS             complement(17360..18394)
                     /locus_tag="PK105_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60371.1"
                     /translation="MRNQGASSTALKVLLAATLSIILATTVLLLFAADFRSALWQFFV
                     EPFSSRYFFGNYIADTVPLIIAGLGVSIAFRSRNFNLGGEGQLYAGALAGAVLALHIS
                     EAFGPSAVWILVAFIGAFIGAMIGTISGLLKAYWQVSEMISSYLLSAIIINISDFFIT
                     GLLQDRTSNFQTTAQIPSEFLLPRIFAPSKLDISIWFALFLILFFQILSKKSKIGFEL
                     MVCGENEEFARYCGISVRAYNIAAMAISGGLYGLSGALLVIGPQGRVMRGFSSGLGWS
                     AISVALLAQGSELAILPSALFIAFLKAGSDHVMIGSGVPSEIISILQAAAMLFITAQV
                     LPGLGQRRRQ"
     gene            complement(18391..19947)
                     /locus_tag="PK105_01310"
     CDS             complement(18391..19947)
                     /locus_tag="PK105_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU60372.1"
                     /translation="MQGIYRRYYPRGVHANDGASLEVEKGSIHAIVGENGAGKTTLMK
                     ILAGLDQPDEGNIEIEGKFVKIESPSKAQALGIGMVHQHFLMFDELSAAENIFFGIEP
                     HRNNKVSKIAGIVDSQELNARTLQLATEYGFAIDPSAPAACLSIAARQQIEILRQLAR
                     HTKILILDEPSSVLTEQETKVLFEKLSEIKRLGHTIILVTHKIHEAMQIADRVTVMRG
                     GKTVGTYDISETTLEELTSLIMGKSFIEATQKPIEKPQIKNTQKKTIVLHIDGLNVRR
                     HQHYGVGLSDISFEVHAGEILGISALSGNGLDELEDVLAGFRSPSSGTCTFDGHNVEL
                     HNSRRYQSLFQKRLIAYLPSDRIRRGIAPSLSVRDNFIALSRQDFFPKGWILENAAKQ
                     AIVNAIHSFEINALPDQPARELSGGNIQKLSIARLFSRDLPKLFILCEPTWSLDIRSA
                     EHTYRRIRNARERGTAILLLSSNIDEILELSDYVMVLSRGRRVLYQENGNWLNRAVIG
                     EYLLGNRIHE"
     gene            complement(19973..20977)
                     /locus_tag="PK105_01315"
     CDS             complement(19973..20977)
                     /locus_tag="PK105_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU60373.1"
                     /translation="MRRFIIASLVAILTSTALWAQAPRIGVFVPGARDGSPIYDNMAK
                     GAERLVSETPRASLRVTEAGFNQAEWKEKLISFVASGKFDIVITSNPSMPELVNEASK
                     IFPKQRFICLDGYLAGNSSVYSALYNQLEQGYVTGYLSGLVSISGMKGTNPEKKIGMI
                     IGQNYPAMEKMIIPGFLKGLMAVDPGFQLDIRVLGNWYDAAKAAELSRSLYAQGADII
                     LPICGSASQGAVRVAQETGKYLVFFDDDEFARAPDNILGCTVLHQEELAYRSLKAAIE
                     EKLQFGKAEIVGMKEGYIEFLNKNPSYIKNVPESIRIKVDAVIQSIKSGRLTFQVPSL
                     "
     gene            21112..21579
                     /locus_tag="PK105_01320"
     CDS             21112..21579
                     /locus_tag="PK105_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU60374.1"
                     /translation="MPDKLLKIGELASLFRVTARTIRYYEELGLIEASNRTEGFHRRY
                     PADAIIRLKRIEELKAMGLTLGQIREFFILYSQDPSGEKCRLLLLQNYEDQKKEEETR
                     ILEAQRRIAQIEANIIAIKEKKSFFSCPGEECKVCNFNTFCIDSEHNPDKEMR"
     gene            21738..23213
                     /locus_tag="PK105_01325"
     CDS             21738..23213
                     /locus_tag="PK105_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOU60375.1"
                     /translation="MILAVDIGTTRIKAALFDAEGRSFKLEIVDLPDESSNYYEEINA
                     RLWLKAFSEIISRLIATGEKNELKAIVICGNGPTILPIDERGEPLAPAITWLDRRAQQ
                     EAKEASIALNYSLDAAFNLPKILWMRQHLPNIYNRAHLFVSCPEFIAGRLSGEWTTCL
                     PNQGYTRIIWDTKTLEMLRLDIEKFPRFVGVGEIVGYIHDHAAREFGLPNGLPVVMGG
                     PDFIASLLGTATVKPGRTCDKAGTSEGINLCSERDIGDEEALLVMPHIIPPYFNISGV
                     ISSSGIALSWFKSRFLPQEDFETIYARIASAEPGAEGLVFLPYLAGERSPHWDPNARA
                     VFLGLGLNHDLSTMARAVLEGTAFAIREVLDVMESRGATVHDMRATGTPSLSPIWNQI
                     KADITGKQLMVSSFPEPELAGCLAIGRFALGEESTLAEAAEKVFMPAEIFEPRKETES
                     MYCELYEVYLDSYRRLADLFPTISVLQRNNGVQRGRFGTGF"
     gene            23389..23970
                     /gene="pduL"
                     /locus_tag="PK105_01330"
     CDS             23389..23970
                     /gene="pduL"
                     /locus_tag="PK105_01330"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: protein motif:HMM:NF011652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOU60376.1"
                     /translation="MPKMEIVINLSNHHIHVNHEDLEVLFGKGHELTKTKDLIQPGQF
                     ACAETVTIRGPKGQLEGVRILGPERKETQCEILASDVFKLGVQGCPVCESGQLEGSFP
                     FEVIGPAGSIKKERGLIIAKRHIHFDLEAAKHFGVSDKQIVKLKAGEERRAILLNVVC
                     RVHPTYALECHIDFDEGNALGITSGAIGEIVYC"
     gene            24026..25096
                     /locus_tag="PK105_01335"
     CDS             24026..25096
                     /locus_tag="PK105_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOU60377.1"
                     /translation="MNRFILKAMDLDGYLSQSDLEYLSRMNELYEKALVSFNILSTTT
                     SEAQRHHEENFLIELYNQMGKLMQQICASEPRIQVYSFVSPQETHGEVSRLIAKLRDV
                     NTGHQEFVYYIQRAFELLFNLAFGGAGKTNKNYLIVRTPVTIPVQNYAVHKIPNVDSA
                     VHNTVMCVMLRGALLPSMILSKEIQEYSSTGYLTPFALFKIKRDESKSETTMEYVLDL
                     DHSYFELSELDGKHLLFADPMNATGGSLVTVVKYLLEQGVKPASIKFFNVISALKGTL
                     RVIRAIDNIMIYTLWMDPSLNDRAYIMPGLGDAGDRINGSDEDLHPRDMLRLVADYGT
                     NITGLYRSQLRIIEETVLRHRR"
     gene            25093..25998
                     /gene="nudC"
                     /locus_tag="PK105_01340"
     CDS             25093..25998
                     /gene="nudC"
                     /locus_tag="PK105_01340"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOU60378.1"
                     /translation="MTIFPESFVVFQGQDCLIPPQDAYNMRFLDEPSLAASIKPEAVC
                     WHFSQIPSDLLSPSSDHYAYSVFEFDDRKYGAIQIQDNDDILIKRPGLDEAIAFPVRE
                     LVVSYPSRFSRFMLIAKAHGQWASVSRFCGACGSLLVDAKENDEVRERLDDNAYAARF
                     CPRCKRIFFPRMSPAVIVLVYRENSILLGHNVRFPRNRHSLIAGFVEIGETLEETAIR
                     EIREEAGIEVKKLHYVLSQPWPFPDSLMIGFIAEWASGEARPDRNEIDHIAWYGADDL
                     PELPLRGSIARYLIDSFIAGGFSNG"
     gene            26059..27474
                     /locus_tag="PK105_01345"
     CDS             26059..27474
                     /locus_tag="PK105_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HOU60379.1"
                     /translation="MDFSWKIVIDAGLISFALVIATFLRSKVRFLQKFLVPNALTAGF
                     LLLPLYNFLMPAIGYGTNRLGDLVYHLLNLSFISMSLRSSPPKIKGSRSNGSVLGMSA
                     VILLGYASQGLLGLVLTLFFLPNIHPAFGLHLPLGFALGPGQAYAIGKGWESMGFEGG
                     ATVGLTFAAIGYLWACFGGMLLIHKGLRLGWMKPEQVSIMSDKALLTGIVPNGTEKPV
                     GERLTTDSEAIDSFSFHFAVVVFIYLLSYLALKGISFLLGFVGEAGTELAANLWGINF
                     IFSAIIAIIVRKIMDLLKVGYVLDDDTLSRISGTSVDYMVAGALGAISLVFVGKYWFP
                     ILLMSTLCGFMVYYTIPWVSSRIFRDFRFERMLMLYGVSTGTLSTGLALLRVMDPEFK
                     TKVTSDYMLSAGLTFVLAIPFILAINLPAKAYTSGSMVSYWIFMAIAAGYMLFVLVVY
                     LILARKRRFASPSANFYRPGK"
     gene            complement(27456..28736)
                     /locus_tag="PK105_01350"
     CDS             complement(27456..28736)
                     /locus_tag="PK105_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU60380.1"
                     /translation="MILNRRYTPTNFRFFTGLLRFTYGNWLKLRYRITAINTQLFKEL
                     KPPFLLVGNHTTILDPFIANAFVPFPVHWVASDGNMRNPIIRFMLIKLVGSIPKSKAI
                     PDIETVNWIVDLISHKHGVVGMYPEGQSTWTGTSFPVYSSSAKLLRLLRVPVVLVKTN
                     GGYLTKPRWSHVLRQGKVEISFSLLFSPEQLGAMRLSTVDRLLNEALSYDDSAWCKRK
                     GIEFKSTKGAESLELAIYLCPRCGERASMHSQGNLFTCKQCGFAVEYQHDGSFLLHSQ
                     GDREDYIPPFVEAPFLDSIVSWDSLQSRLLYQYLSGLKSAYLEAPFFLDEPIKLKRGK
                     RMDHMETLLNGRIELYRDRLEFHNTRNTKLDPIVFPIEQIDGEGVLKWNYFEFYHGMN
                     VYRVVFKDKRASGRKYADAISVLHQISKSHLPGR"
     gene            28928..29395
                     /locus_tag="PK105_01355"
     CDS             28928..29395
                     /locus_tag="PK105_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOU60381.1"
                     /translation="MPCKKPLSSSNSDQKISENSVSQTLERWLEVHEPVDKDKTNEDL
                     EADKRLDSISAYSRFKRILPQDELDLHGLYAEEARQRIQAFIADSASRGLEKVSIIHG
                     KGNHSHEEQVLINIVREELEKNPQTGEFDFADRKHGGRGATWVRIRPPISRDR"
     gene            complement(29376..29594)
                     /gene="infA"
                     /locus_tag="PK105_01360"
     CDS             complement(29376..29594)
                     /gene="infA"
                     /locus_tag="PK105_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOU60382.1"
                     /translation="MAKEEAIEVEGIVKEALPNTMFRVELANGHIILAHLSGKMRKHY
                     IRIVPGDKVKVGLSPYDLNRGRIIYREK"
     gene            29859..30215
                     /locus_tag="PK105_01365"
     CDS             29859..30215
                     /locus_tag="PK105_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOU60383.1"
                     /translation="MGNAFIDRMKNLLLAQRQEILNTIAESNKELRKALSETDPKDSA
                     DIASDDIDRIMIEALSSQDLKRYQAIESALLRINQGRYGLCAKCGKKIPEERLEAIPY
                     AVLCVECQKSDERKNR"
     gene            complement(30217..31101)
                     /locus_tag="PK105_01370"
     CDS             complement(30217..31101)
                     /locus_tag="PK105_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOU60384.1"
                     /translation="MLVSGMAFSFHTHTLFCDGKADATTMARTAFEHHYTHLGFSAHA
                     PISLETDCNLPWDEADSYVYAIRSLAQEYEPKGMKIFLGLETEYVPGIAKPDDPAYEP
                     MQLDYRIGSVHYVTTPGEYPFTVDEPEDQFALHVQDWVPDGDYRKIWKRYWCFISEMI
                     EAGGFDIIGHFDLVKKNNIAGRWFDEENRAYTDAAFQAVDLAAEKDYIAEINTGGIAR
                     SKRGEPYPSLFILKRMQEKGIRITIGDDAHTPSHIGVYQPKAMDIAEMAGYKTLWYMD
                     APGVWKEVTLESVKAQLR"
     gene            31215..32675
                     /locus_tag="PK105_01375"
     CDS             31215..32675
                     /locus_tag="PK105_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOU60385.1"
                     /translation="MRESSARSIGVFLSEISKGEKLARSLRECGASVAILNGYSEDLS
                     RQFDCIVAEIGLLNEREIQAILDSSTALVLIEKAVEELHRTEDAEYSLGQFSNSALLR
                     ILPRNATSSRIKCTIYQLFAKKDSLLSSKNTIAAAQSQLRTLLSSLPDIVYVLDEQGY
                     FLYLNESIGLLGYLPSELVGKHFGTIIHPEDRPNISRDLVVEKIRQDNIMPANPPKLF
                     DERRSGSRMTRELEVRLLHKDGHVIYAMVNAYGEKAFDVPLFSDVVGKKLQTIGVIHD
                     ISAMHLYQESLEESLAAKERLLREIHHRIKSNLQLVASLAHLKQIDAQSSAAIEVLRD
                     LETQIKSIALVHEALYQSERIDQLSARDFFGQFCHFAEEALERVGSTVQIRVSAEDYP
                     LDPNLLVPLSLALLYLLEGAYKVSYDRHEKVEVSLSFMRGQDGMQIIEMNGEGILENA
                     NSIVMQALLRQAHTSLIIPNTEKGRPSCHLIEEAKS"
     gene            32732..33673
                     /gene="trxB"
                     /locus_tag="PK105_01380"
     CDS             32732..33673
                     /gene="trxB"
                     /locus_tag="PK105_01380"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOU60386.1"
                     /translation="MHQIYDFAVIGAGAAGLSAAQYGARANLNTIVIEELAPGGQALF
                     IDKLENYPGILEPINGLELAERMQRQAEQFGSSFFEASALSVRKEKGHFIIETSAGPL
                     ESLTVLIATGARHRRLDIPGEQEFVGKGVSYCATCDGPFFKGKRILVVGGGDSACDEA
                     IYLSKLSDKIVMIHRRDRFRAQKALSSRVLSNPSIRVLFNTVAKEIKGDSKVRSVVLE
                     NTISHELTEEPFDGIFIFVGSTPQTAFLKDIGVQFDESGYILTNPNMQTNIAGLFAAG
                     DVRATPFRQIVTAAADGAIAARSASDYIDEQRGQTYR"
     gene            33691..34353
                     /locus_tag="PK105_01385"
     CDS             33691..34353
                     /locus_tag="PK105_01385"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOU60387.1"
                     /translation="MKKSMNSCTLDSVLRIPIELVCGVDEAGRGPLAGPVAAAAVILP
                     KGFPIAILDDSKRMSARRREEAYCIIVEQALDWAVGWATVEEIEKHNILMASLRAMER
                     AFGGLSHLPALVVVDGLFAPSLSMNGTNIEAATMAKADGLIPAVMAASILAKVARDRL
                     MDCLDCIMPEYGFRKNKGYPTKAHRAAIERFGPSRFHRKGFQLLIQSDAPLFDSLESP
                     AE"
     gene            complement(34305..34709)
                     /locus_tag="PK105_01390"
     CDS             complement(34305..34709)
                     /locus_tag="PK105_01390"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOU60388.1"
                     /translation="MGIRGELFSTRFICEGRTYFFNVKENRNGDIFLSIVESKPTETE
                     TFDRRSIVIFQENMEGFMRALRTAAGYMEKASQRPKPDRAAIQSSRTRQEPRQTHQAL
                     HESSSRAPRRFVIKKKPHSAGDSRESKSGASD"
     gene            complement(34731..36707)
                     /locus_tag="PK105_01395"
     CDS             complement(34731..36707)
                     /locus_tag="PK105_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase"
                     /protein_id="HOU60389.1"
                     /translation="MSLITHPFLGLPIGDPGKIPLSREELPSFEDIRLATQELILSPS
                     GWRKIFAKPELAAGYASWDYEQLPDNSLSPLLAPTDMVIVAGMALVFSSFIKEHCKPH
                     KPSILLGIDTRPTSPAIADIFARVLIGEEYEVRYLFIVPIPEITAYAAHVTTLDVDNE
                     FHSDGFVYVSASHNPPGYNGIKFGLGGGVLTGEQIRPLISDLKLFLSDPESSEIALMA
                     LEKADSRALTKCYENVAHWKRYSQSAYMLFAHRVFTDEETAEAQERILLEIAEACEKR
                     PFGIIAELNGSARTQSIDLDWLSGIGVQTRALNIQPGVFAHRIVPENESLDDCRKALQ
                     LAHNENAAYALGYVPDCDGDRGNLVYYGNRLGEAIPLEAQQVFALACLSELAYMQWKG
                     EKNRIAIVVNDATSMRIEAIARVFGAKVFRTETGEANVVCRAEKLRAEGWTVRILGEG
                     SNGGNITHPSKVRDPLSTIGSIIRLLRLGDAEKKETCFNLWLEAIDSPERYQSGYNLE
                     DVIESLPQWITTSAFEPHAALKIHAVDKIELKKAYQRLFLEEWPKMLPELEQRFDIVS
                     WRAFASLGPDEFEVESDFGSSKNGGLRIVLYDKADEPRAFLWMRASGTEPVFRIEVDI
                     KNGTCSDEAWLRRWHAGLVTEADLLAAPRQNNVG"
BASE COUNT         9829 a         8203 c         8522 g        10185 t
ORIGIN      
        1 atagtagccg ccaataggag ggatcatgaa gattctgaaa tttggtggta catcgacagg
       61 cagccctgaa gcactgaagt cgctcgtgcg aatcgtgcgg caatctggag aaaagatcct
      121 tgttgtgtcg gctctttccg gcaccaccga tgcgcttctt tgttcagcaa agttggctag
      181 cgaaggtgga gactggcaat ctgtatttgc ttccattagg gagcgacatt attcgatgat
      241 ttcggctcta ctgcagcccc cttataaaga gatcgccaca ggtgagacaa gagagctact
      301 ggatgagctt tcttctttgc ttcttggtat ttcattggta cgagacttat cgccaaggac
      361 tctcgatttg gttgcgtcct acggggaaag gctgagtgct ggtataattg ctgagacatt
      421 ttctcagaca ggcctgattg ccgaggctgt ggatgctcga aagttcataa taacagacga
      481 taagtttggc gctgcgcatt ggctggaatc tgaaacgatc ccacgtgtcg aatccttttt
      541 ttctgatttt gagcatattg ctgtagtaac agggtttatc gcctcgacga ttgaaggaat
      601 aacgacaacc ctgggtcgag ggggatcgga ctttactgct agcattttgg cagcctgcct
      661 tgaggctgag gaaatccaga tatggaccga tgtcgatgga attatgacag ccgatcctcg
      721 tgcggttccg gacgcgtttg tcataccgga gatttcctat ctcgaagcca tggagatgtc
      781 tcactttggt gcaaaggtct tgcatccgcc tgctattctg cccgctatgg atcgaggtat
      841 tcccgttagg atactcaata cttttaaccc ggataagcca gggacaagga ttgtaagaca
      901 aggagcacca agtagctggc ctgtgcgtgg tattgcctcc ataccatcca tatctcttct
      961 cttgttacag gggccgggtt tgccaggcgt tgctggaatt gctgcgagaa tgtttgctgc
     1021 ccttgcctct gcaggcataa atatcattct gataacgcag ggatcgagtg agctgtccat
     1081 ctgctgcgct gtgcttcctc aggattcggg gaaaggagtt cttgctctta aagaagaatt
     1141 caaatatgag atcggtgcgg gtaccattca gaatccttcc atcgagcaag atctcagcat
     1201 aattgcggtt gtcggcgaaa tgatgaagca cagagttgga atctcggggc gtgtcttcaa
     1261 ggctctaggc agaaatggca taaacgttgt cgctatagct caaggatctt cagagctgaa
     1321 tatctccata gcaataagtg ttcaggatcg aaataaggcg atgtgttcta tacacgatgc
     1381 ttttttcctt gcgggagttc gtacggtcaa tgtgtttctt gtgggaacag ggctaatagg
     1441 tagcacattg cttgcacagg tggcagcaca gcatagaagg cttttccgag atcggtcgat
     1501 tcgattgcga atagcgggca tagctaattc gcggcaaatg ctgatagatc ccaggggaat
     1561 cgatctagaa aactggaagg atgcattgca gtcgggaaag aagaccgata tttctgcatt
     1621 tatccaagag atactcagcc tgaatttacc gaatgcctgt ttctgtgatt gtacagctag
     1681 cgaggaattg gcacagcact atgaaaaact cctgtcatct tcgatttcgg tcgtgacacc
     1741 gaataaacga gcaaatagcg gtcctttgcg cgaatatcag aggctgatgg atattgcacg
     1801 cgagatggat gtgccttatc gttacgagac aacggtgggg gctggactgc ctgtcatcgg
     1861 cacgattcaa gacctgatag catcgggaga cgaaattctt cgaatagaag cagtgttatc
     1921 tggaaccatg agctttattt tcaataacct gggggagaag cattcttttt caagtcttgt
     1981 tgcggaagct aagctacgag gatttaccga gccagatccg cgcgatgact tgagtgcaaa
     2041 agacatagtc cgaaaaatga taattctggc acgcgaggcc ggttttccat tggaagaatc
     2101 gcagatacag atcgagtctc tccttccaaa acgaataata gatgccccta ctgtggaaga
     2161 attcttggcg ctgctgccag agatggatca aatgatgaaa aaatgctacg attcagcatc
     2221 tcgatccgga gcggtcttgc gctatgttgc catagtaaca tctagagatg cgcgcctttc
     2281 actctcctcg ttcgggccag ccagcccttt ttatagtctg tcaggaaccg ataatatggt
     2341 ggttataact tcaaagcgtt attctcaaaa tccgctcgta atacggggac caggtgcagg
     2401 agcagaggtt acggctggcg gagtgtttgc cgacattctc aagacagccg aatcttatct
     2461 ctgataggtc tcgataagga aatataaaaa gaggctgcca tgtggcagcc tcttttgtaa
     2521 agctcttagg atcctttagc gtcgccgcta tattgatgtt ctatcgaggc aatcaagacg
     2581 atgcttttgc tttcagtttt ttcgagcgca taaactgact aatagacaac aaggctataa
     2641 gtccgattcc tatgatgtca gtcttaatac ccggatctat caataatact ccgcctacaa
     2701 agccaattat tctttcgtac catacagtat tcgtcatgag ccagccattg accgcagacg
     2761 cgaccgaaat tatgcctaca aggcttgtaa ttagcatcca gacgaatgag aataaattga
     2821 catcgaacaa gagcatctga ggattcatca caaatatgta cggcaccagg aaggcggcaa
     2881 ttgcaagctt tgatgcattt attcccgttt tcatgggatt tgcttttgca atacctgctc
     2941 ctgcaaaggc cgccagagca accggaggtg tcacgtctgc aattatacca aaataaaatg
     3001 caaacatgtg ggctggcaga ataatattcg cgggcgctac agcataagga tctaagcctg
     3061 cttttctaga aagaagcata atgactgcag gagcggtaat agtcgaagtt atgacatagt
     3121 ttgcggtagt cggcactccc attccaagga taagcgatgt gagcattgta aagaagagcg
     3181 taggaatgag cattcctcca gcaagatcga ccagaacaga tccaagttta agaccaaggc
     3241 ccgtcagagt gacaatgccg attatgatac cagcgcaagc ggtggccgcg agtactccta
     3301 gagctgcacg ggcaccagcc tccaatgccc gcacaatttc tatcggagga atgcgcgtac
     3361 ctttcttgat ccatgacgat attatggaaa gaactatagc ccatagcgca gctctcatcg
     3421 gtgtatatcc cgatactaac aaatagatta ttgctattaa aggaattatg aggtgtcctc
     3481 tttccgtaat gacctgtttg agttttggca actcttctcg actcagtcct ctaagcccat
     3541 ttttcttggc ttcgaaatgt acacctgccc atacaccaaa atagtagaga atcgcaggta
     3601 ctacagctgc gccaattatt cgagaatagg ggataccggt gaattccgcc atcaagaaag
     3661 ctgcggcacc cattattggt ggcattaact ggccgcctgt agaagccgtg gcttccacag
     3721 cgccggcaaa ctcaggtttg tatccgagac gtttcatcat cggaatagtg aatgagcctg
     3781 ttccgaccac atttgctact gatgagcccg aaacagtacc caatagggcg cttgatatga
     3841 ctgccacttt tgctggacct ccagctgccc agcctgctac ggaatttgca aggtcgatga
     3901 aaagttgacc gaggccagtt ctttcaagga atgctccaaa aagaatgaaa agaaagataa
     3961 atgtactcga gactcctagg ggaatcccaa aaatgccttc ggtcgtaaaa tataaatgtt
     4021 gtataagagt ctcgaaattt gcgcctctat gtgctagttt gccgggtata tatgggccaa
     4081 taaaggcata aataataaag acaaccgcga ttaccaccat tggaataccg acaacacggc
     4141 gcgcggcttc tagcacgagc aatattccta ttatgccaaa gactatgtcc atcggcgtgg
     4201 ctgttcctgc tctcaatacc agcttttggt agttgattat gatgtacata ggagcaagag
     4261 cccctgccac cgcaagcaat gcatcgagcg gatgtagctt tgtgaaggac catttcttgc
     4321 tagcaggata aaggagatag ataagtgcca ggccaaatga aagatggacc gatctctgga
     4381 gcatcgcatc taagactccg aaaatcgctg tatagagctg aaacacggaa aatgcaatcg
     4441 ccaatgccga aatgaattta gcaaagaaac ccgaatatat cctaaaatta gactctttgt
     4501 cgaatttctt caaaatctct tgagcatcag tgctggtttc ctgctcatga attggtaaat
     4561 cactcatttt ggagaactcc tcccattgta aaatatcagc attctccctg cggagagggt
     4621 aattcttgat tctggcagca cccactgcgt aaaaagaaaa agttcgtcct caacgattac
     4681 tccatgttga ggaacgatcg acaccaggat ggggatatcg aagaatgttc ggtgcatttt
     4741 gacaacaaaa cgaccatctt caatagcaaa agcctcccca ccatcagaag gcatgccaac
     4801 gccatatgtg tcatacttta cttgaaaaag ctcgagattc cttcctttta tatggaagta
     4861 ttcatcgacc gcggtcttat ggatcgaatg aatataatga tggataaatt gtccatcatg
     4921 gagaggtaat cgtgcaacaa ccttatcttc gccattcttt atgatcaaga ccggcacgac
     4981 cctaagattc atagcgatag cactcagggc gatgatcaag atcaaacacc ccaaaagcaa
     5041 attcttgcct cttttcctcc tttgtttcat attgttcttg tcttaataac ttaataaacg
     5101 gcgcgaaacg agtaggttcc gcgccgtcca tagcgtttct aatgttcgtc tccgatgatc
     5161 ttaatcttac ttcagcgcac ctttttcctt gaagaatttt tccgctccag catgcagtgg
     5221 tatcgacatt ccctcaaggg cagattcgat tttgacattc tttccctgag aatgtgctgc
     5281 aattagtctg tcggtatttg catacatggt ttcaagcatt ttgtacacca agtccgaact
     5341 cagcttgcta ctaacggcga gcatcgattt tacagctact gtggttacag cttggtcttg
     5401 cccctggtag gtacctgccg ggactacctg caacgcatag aagggatatt gtgccatgag
     5461 ctttgctgcg atctggccat ctataggcac gagcaccatc tttttagaaa ccgcaagatc
     5521 ttgaatggct gccgttggaa ttccggccgt attgaaagcg gcatcgatat tgccatcctt
     5581 catgttgttt gcggattcgc caaaagacag atactggacc ttgatgtcat tgtaggttag
     5641 gcctgcagcc ccaagaattt ggcgagcatt ggcttctgtg ccagaccctg cggcgcccac
     5701 agacactttt ttgcctttta gatcgccaat ggtcttaatg ccgctggatt ccagagcaac
     5761 gagctgaatt gtctcgttat atagacaagc catgccgcgt agatccttat aagcttgatc
     5821 tttaagcaat tccgtaccgt tgtaagaata gcttgcgaca tcgttctgtg tgaaaataac
     5881 atcaacattg ccccctttga gcaggttgat gttggcaatg gaagcgccgg ttgcctgggc
     5941 tgttgcgttc atcttggaaa tattcttatt ccatatgtcg gcaagagcac caccgagcgg
     6001 gtaataagtg cccgcagtgc caccagtcgc aattgtaatg aattgttgct gtgcaaatgc
     6061 gctagttgcc atgagcaaac aaattccgag tactaagaat cttttcatgc ttctttacct
     6121 cctgtacaaa aaatcgggca tgaaataaaa gctaagattt tagaattata cactatgttt
     6181 ttcgcgaatg caaatattaa atggtgttgc tcaacatgtt gctcactgtg ttgactcacc
     6241 ggtgttgctc atcctgcctg gattggatag agtaataaca ttggattatt cgggcattat
     6301 ggccaagatt gagggctaga aggctatcgc agagatatcg gagattacct ttgatttcat
     6361 ttgagaactg gaagcaaggt tttgtaataa ccattgtagg ccagcggctt ctatggcata
     6421 cgaggaaacg gccggccatt tttgttcatc gatcaggcga aatacgttct gcatggagaa
     6481 gtgtagggag ctgcactcca tccatggctt cgaaggatag cgcattcttt atcttttcag
     6541 agggcatctc aatgcgagaa gaatttcagc gatatggaga acaaggctcc gtttttttgc
     6601 tggagttttc ttcgatagac tcaaagatcg atgctgttaa gattcgcttt ctagataatt
     6661 cggccgatga atggaatggg cttggtcctc ggccatctca tagcttgaag ggtaatatta
     6721 atctaaagca gggcttgcac actgctgcat ttgctatcga tggtagattt ctgctatgcg
     6781 aatcgtcggg aaagcgactc ttttctttta aggatggggc aatagaattg ttgctagaat
     6841 tgcccgctgc cgtgtatctg ggctatgcta aaaaccctat tgaagcttgc aggctttggc
     6901 atatctggac aatgccggat agacatttag cccgatggat tgaggtacac aaggaattgg
     6961 agcctcaaga agacataggt agccttaagc ctggaaatgc tgcgggatta ctgggatcgc
     7021 tctctttctc cggagcaagg cccaatgact ttcaagaatt gaatttatcg atagcagcaa
     7081 aagcagaagg gcgcgaggat tggaagatag cggatctaat gctatgtcag cccgatattg
     7141 ctcttcctct tctgtttaga agagaaattc caatgaacct cgaactcggg aacaatagga
     7201 aacgtgctca aaatcgttat gcaaagatac gggctggtct tgctcagtat tggcaattct
     7261 gcgagaaaag atggacaaat gaaggaatac cagccctttt tcatccttcg attctatttc
     7321 cagatgattg caataatgcc gagagcgata atatgctctt ttcagggcct gatcttgtgt
     7381 ttgcgccgaa tatggcgagc aaagatgaat tctgctcgtt gattctgcct aagggaagtt
     7441 ggatacacct gtggacatcc aggatctatc ctgcaggaaa ggttaccgtt tatgctccat
     7501 ttggtatgcc agcacttttt taccggctag agagcgaata tgcgtggctt tttgattcgg
     7561 tcaggcagag agccagcaga ctatagatta tgaacactat caccgcatat tttgccgcac
     7621 tttttggaaa cttcctcttg tctctaggct tttcccttca aaagaagcat gtgcattggt
     7681 tgtctgctaa gcgtcgcggt gaaagcataa ggcgtggtga gataataggt tgggcaagtg
     7741 gttttgtcct gatgaatgtc cagccaatct tcaactatat tgctctcgag actttggcac
     7801 cgaatatagt agcagcattg ggtggttcga acattatctt tacgattgtg ctttcctatt
     7861 ttctgctcgg cgaacgaatg ccccctcgaa aaattccttt ttttgtactt cttatggcat
     7921 cgctagtata cgcaggtgtg gtcgggcaag aagcatcgcg gcagtttaat gtacaggcat
     7981 tttgggtagc attttttatt cctacggctt tttctatgct ggttcttttt gcaagcaaga
     8041 gaatgtctga ggacagaatt ggactttttc ttggaagctc tgcaggagcc ttaggaggat
     8101 tcatggttct tgctctggaa gcgcttagaa taaactatgg ttcaaatata ttgagttggt
     8161 ttgtgtccgc atatctctat gtttattttt tctgcggaat tgcttctttc tttatcaagc
     8221 aatttgcctt cgagcgcggg aagatgactg ctgtggcacc ttctttctat ggcttgcttg
     8281 tgctttatcc ttctattgca tcctattttg tgtcaaatat gccggttcat gcctcacaac
     8341 tcatcgcttt tgggggaata agcgtggcaa ttgtcttaat ttcgatataa gtgatttatt
     8401 gggtgcaaag acaaggattc gacaaagata caaagataca aagatataaa gatataaaga
     8461 tagccatagg ctctcgtggg cttcagcgac aactagcgac agatcattac taccgcatgc
     8521 gcttctattg cctcaccgcg accagtggca tcgaagccct cttttgtttt tgctttcaag
     8581 gagatggatt ccattgaaag ctcaagatta gcggcaatcg aggcctttat agcttctcta
     8641 tatggagcca gtctgggttt ttcgattatt attgtgcagt ctatattgga tagttgccat
     8701 cctttgctgc gaataagcat gaaggcctta cgagcaagaa cagaagaatc cgcatccttc
     8761 cactcaggct ctccaggagg aaaaagcaaa ccgatgtctc ctgcggccgc cgcaccgagc
     8821 atagcatcga taattgcatg ccaaagcaca tcaccatcag aatatccagc ttcgcctttt
     8881 tcactttcga ttcgaacacc tgcaagcaga agaggtcttc catctacaag cggatgaata
     8941 tcatatccta atccgatgcg cagaggcatt ggaaaggaaa gcgattcatg ggccaacaaa
     9001 cctgacatga gatcttcctt aaaagtgatt ttgcggtttt tgcgttctcc ctctatccac
     9061 gagacaggac caacatagtg gtcccagatc attgagtcgt ccgttgccct aaaaccttct
     9121 agatgtgcta gctcgtgcgc agcaagaatc tccatgagag aaaaagcctg tggtgtctga
     9181 gcgcacatta tgattcccct tgcgggatga tcatgtataa gatcatcttc tacaaccttt
     9241 ggcgtttctg tcagcggcat aagtggcagg caagctcctc tttgccttgc gcagcaaagg
     9301 gttttctcta tcagcttctt agaaatccac gggcgagctc catcgtggat cagaacaaca
     9361 ccatcattat tacaatgagc tgcaagttcc ctgagaccaa agagcacaga ttcttgtctg
     9421 cttgaaccac cctcggttat caagaaccca gcttgaaggc ttgctaagtc cggctttaat
     9481 ttggattttg aaaatagctc ttcgaactct gatttttctc cttgtgggca agtgatcaga
     9541 atcgcattac aaaatgggat aaatggctcg acggctcgct ccaaaacggt aaacccacct
     9601 atgtcttcca attctttttt agagcttcca taacgcgagc tcgatccagc agccgtaata
     9661 agcccgaagg acggtccctc aatgggtagc ctgtgaacac tgatggtttt ttgaacagat
     9721 gagctcattt ttccagattc tcgcgaataa tcgcggctat ctcttctttg ggacgatcta
     9781 gcgcaaaggc aatttcatct tgtagtagat tgagtgctga ttcatacaat ttccgctcaa
     9841 gaatagggag ttctttaatc ttggagcgct gatagagtga gcgtacaact gctgcaatgt
     9901 ccgttatcga acccttctta agtaagtcta gattcatctg atatctaagt ttccaatccg
     9961 aggggatagt ggacgcggct ttagatatcg aatccagcgc cacctcagcc tcctttctgg
    10021 agacaagcgg gcgtattccc agtacttcca ccttatcgac aggaaccata acagtcatat
    10081 ctgaaaattc aagatagata atataataaa gagtcttctg gttattaaag gtcttttctc
    10141 gaatctccat tatgcgtccg acaccctggc ttggatagac cacacgttga tccactagaa
    10201 aagttgtagt ttgatcattg ggcatagttt ttctatgata gcagaaaaac ggatgatagt
    10261 caaaacaccc ttattaggcg tcattcgact gtgctcgagg attttctagc ccctagggga
    10321 atcgaacccc tctttaaaga ttgagaatct tttgtcctga nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnccac aagcagatcc agagtctgct gtgctaccat tacacaaccg gggaatgacg
    10441 ccctgtacct tagtgatttt tgtggttttg gtcaagagtt gctctgctat aagcttggcg
    10501 attcagagcg atggacatca tataactcga gttgaggtcg ctcttcgcca ttccaataat
    10561 ttgtagtgac tttgtaaatg atgtcgactt tatcgttgtc tctgaatgaa aaatcccgtt
    10621 cgagtctttt ggctccatcc caaagcatgg caggccattt atatctacca aaatcaagcg
    10681 ttagttttag atggctcttg ccatttttcc cgactatctg agcatcaacc atagagacat
    10741 tcctcgaaca gaataccagc ggatcgctct cttctcctat cggttcgaag taacggcata
    10801 gctgaagcag atcgggagtc acaaatgaat gaggaagttc agcatctatc aggaattccg
    10861 gttcttctgt tataagctcg gtacgagcca tgaattgagc aagtcgctca agaaaggcct
    10921 gccagttatc ggtcttcatc gtaaaaccag cggcagagtc atgacctcca taatcaagga
    10981 atagatctgc acaataagct aagatctggg aaagaggaaa agcatcccca ccgcggatag
    11041 aaccggtaca gatgccgtct gttttaaata ccgcaacaat cacaggtatc ttcatgatat
    11101 tagccgcacg agaggcaagc agtcctgtga ttccactttt tatcctggaa ctgcccacta
    11161 tggcaaattt gccgccgctc gttttgacac tctctgcaag ctgatcttga atcagtcccc
    11221 aggcctcggt tccaagtcgc ctacgctcgt tgtttgcttg aattagctgt agagctgttt
    11281 cggtggcttc gattgcggtt ttagcttgaa atagatcgag agcaagtttg ggtctaccaa
    11341 gtctgccagc ggcattgatg ataggcgtga tctgccaggc cacgtcattt gatccgagtg
    11401 gctttccaag attgagcgcc atctttagct ctcggattcc attagttgga gcagtgttga
    11461 gactttctat tcctctccgc acaatcagtc tgttttcatt ctttaggggc ataagatctg
    11521 cgattgtgcc aaaagcggtg agttggaaca ttctatcgtt caaaaggctc tggctctgaa
    11581 gagtatggag caagtaggcg ttgaaaatat gcttgagtgc atcgatagca ccaaaagcac
    11641 catgagcata ttttttcaat ctggaaagcc gaacgagtcc ttccagggag ttcccagaca
    11701 tattaggcca gacagtcgca gctagtttct cgagatctat aacttcgagt tcgacgagac
    11761 ctgcaaagtt cttcttgaaa aaggcctctg ttgtattctt attccagaca atcacaatac
    11821 ggccacgaag gaatttttct agcttttcga gcgtatccga ggggaaatat cctctattgt
    11881 cagaggtaat tttcaaacgg ctcgcctcga ggaggttatc gagcctgatt gcttcaatct
    11941 caagattggc ttctttctcg gtattgagct tgcttgctcc cgcacctggg cagatttcct
    12001 gatgaatagc ttggccagcg cgtagaagag caagcggttc tttacaaatg cctagacgag
    12061 ctatagcaag cgcatgagct agctttagag ccacgccgca gccagataga tctctaaagg
    12121 gatagccaca atctgggagt ttgggatcta ggatggcgat ggcttttgga ggttcttctg
    12181 tctgtatacg gtgatgatct gttattatga catctattcc ttcttggctc gcgtagagta
    12241 cttcctcgtg attcgaaata ccacagtcta ccgtaacaat gaggctcaca ccttgttctt
    12301 tggcaaagtc tatcgtagac tttgaaagac catagggttc ctcgccttcg ggcacttttt
    12361 gaaatacctc cagtccaaaa tctctaagag cttcggtcat aagcactgtc gaggtaatgc
    12421 catccgcatc ggaatcgcca aatacaagca ccttttcttc ttcttctcgg gctgtgataa
    12481 ttcggtctac cgcatcctcc atgtcattaa ataggaaagg attatggagt agctgaagat
    12541 cgttctcgag atagaactga atttgctctg gatcggtaat gtttctgcgc acaagaattg
    12601 aggctgtcaa agtatctact tcgtagcgct gagaaagacc tcgcacttgt actggatcta
    12661 tttctctttt tatccacttc atgatacggt aatttcttca agaataattg aggatgcaga
    12721 tggagcgctg tctcctatgg aaatggcatc cagagcgata ggaaaagcgt cttccaggct
    12781 ttggctgtct ttcccaaata gcgtcgcaat acgttcaccc tttgtcactg ggtcgccgga
    12841 ctttttatca aagataaatc ccgccgtggg gctgacacag tcttctgttc gattccttcc
    12901 tacaccaagg tatacacctg cgagtccgat tctgagagca tcaatggaaa cgatatatcc
    12961 atccttttca gccctaattt ccatagagaa ttcggagcgc cacgatcctc ttcttttttg
    13021 catctcttcc agatcgccac cttgccttac cacatttcga tagaaaagct tgagtgcggc
    13081 ctttgattct attgcttctt ggcatagatt ctggcattcc gaaatatcct ttgctttgcc
    13141 tccaaggagg agcatccacg ctcccagcca atatataagc tccattacgt ccttaggtcc
    13201 cttcccttcg agacagtcca gtgtctcttc aatctcaaga aaattgccca ctttgaatcc
    13261 caggggttga gacatatcag tcaatactgc gacgactcgt tttcccatag tgttagcggt
    13321 tgcgacgaga cttttagcca gagctttggc atcatcatag cttttcataa atgccccgga
    13381 gccgcatttt acatcgaata cgataccttc tgcgccttct gctactttct ttgataatat
    13441 cgaagcagtt atgagaggga tcgactcgat tgttccggtt acatccctta gcgcatatag
    13501 cttcttgtcg gctggaacga tatgcgcagt ctgacctgtc attgcgaagc cttcttctag
    13561 caggcctttt cgaaattgcg cgggttcaag gctggtcata tagcccggga tggattccag
    13621 cttgtcaagt gtccctcctg tatggccaag ggctctgcct gacatcatcg gcacttttat
    13681 tccgcaagct gcaaccattg gagcgagcgg tagcgatacc ttatcgccaa ctccacccgt
    13741 agaatgcttg tctatgaaag ggcctggaat tccgcagaga tttattctct ctcccgaatc
    13801 tagcatagcc tgggtaagag cagctgtctc ctgtgcagac attccttgaa agaatatcgc
    13861 catcaataga gcggaaacct gataatccgg tatctcgcca ttcacatagc cttcgataaa
    13921 aaagcgaatc tctttttcat cgagcgattc gccatggcgc ttttttatta tgatatctac
    13981 agccctcatc aagattttct cctaaatctg cttctctatt gataaacgaa tgttgaaaac
    14041 cattttactt caaatttgag ttctataaaa ggtctgatca agaattcgaa ggctctgcag
    14101 agggccttct gctgcggctt cggcaagtga ctcgattatt ttgattatgt cagggctcga
    14161 aggaattgat ttcaacagaa gtacggcttg gccataggat tgccttggaa tgctatggag
    14221 acatgtgaga atcttatcgt cgaaaggaag cattactgag gttgaaaatc ttatctgccc
    14281 gtactcttct gcacagctac ggcacagaaa ggagctttcc ccaaccaaaa gtcctcctgg
    14341 aagggaattc ttctctgaaa aaagcctgcc acatctctca catctcaaaa gatcaggaat
    14401 gaggccaagc ggataaagag catcccaaaa gaaagccgac aggaccaatc gagtttctga
    14461 ttcgtctaat ctggcgagct cacgaaggag tagggtaatc atcaatagag cttgttggta
    14521 ttcaccgcca aacgcggagg ttcgaatcaa gaactccgat gccgcgctcg ccgattgcat
    14581 atgaaaaaag ctcttacgaa taccatcgaa aacttcaaga acattcgcgc cagtcagttt
    14641 tataaaatcg cgccgcgaat cacgatatac ttcaatatca gctaatacga aaggcaaagc
    14701 ggccgaacgc agagagtttt tcggtcctcc gaacataaaa agtgataaaa ggccttcatt
    14761 acataggaag gttagaattc tatcgccccg ggcagtgtcc tttgtatgga gaaaaaggcc
    14821 tctatatctt tcgtttctct gagccatgga tcaatcatca tccacaatcc accaatggaa
    14881 gccatcggag tagccggcag taggaccttt acgccattct gcaatgcgtt tcgattcctt
    14941 ttttttatag gatttgcccc atatataggc gcttaagaat cctcctatat gtgcaaaata
    15001 agctgtgccc ccattctgaa tggccgtaag gccactcaac cctccgataa tttgcataaa
    15061 aaaccaggtt cctatgacaa accaagcaga aaataccgtt ggtatgaaat aaaatagaag
    15121 tacgacaact ctattgccag gaaacaaaac gagatatgta gcgagtacgc ctgagatcgc
    15181 ggcagaggca cctacaagag gcatttgaag cgcggcgcct tttgcaaaag cagcaaggaa
    15241 tatctgcaag agcattccta ttgttccgga gatcaaataa agcagaatat atcgaagttt
    15301 gcctattcga cattcgacat tatcaccgaa tattccaaga aacatcatat ttccgatcaa
    15361 atgtgcaaac cccgcatgag tgaactgact tgtaattaga gtgaacaatc gtcttccaga
    15421 caaaatctct tctggaatcg ccgcaagagc cagagttgta tagtcgtcta agccaaattt
    15481 ttgcaggaaa acaaaaacca atatatttag agcaatgaga atccagttta cgatcgcact
    15541 gccgcgacga caagtattat cgtctccaat tggaatcata gaagctcctc ttcaaatgcc
    15601 actctattta gaataacata ttgtcttatt atagactagt tttttccttc gcttgtcgcc
    15661 aatgtgcaat tcatgataca aaataaacac atgatctgct ccttgctatt cgatctcgac
    15721 aatactctgt attccgaagc aactggcctg gaaaatgggg ttgcacagag aataaatctt
    15781 tttattgctg atttttttgg gctctctatt tctgaagcaa tcagggttcg acgtgagcat
    15841 gcaaaagcat atggcaccac cctcgagtgg ctcatgcgag attatggatt ccaggagccc
    15901 gaagaatact ttagggctat tcatccggag gatgaagcgg attgtctcaa tccggatatg
    15961 gtgctaaagc tcatcctgaa caatataccc ctaccaaagg ccgtgctaac aaatgctcct
    16021 atggatcatg cgatcaggat tcttgataag ttgggtatta gcgagtgctt ctccggaatt
    16081 tatgatatct ggttcaataa gcttgttggc aaaccacatc caggttccta tcttggcgtt
    16141 ttggaagcct cgggattcac gctggagcat acacttttta tcgatgatct tccaaggtac
    16201 gtaaaaggct atatcgatat aggagggcta gcggttttaa aagatgaaat ggatcgcttt
    16261 tcagacctgc cctttaggcg cctaaagaca atttatgaac tagatcaaat attagaccaa
    16321 ctcaattctt cttatatata gattaacctt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn cttatatata gattaacctt cgctattgtt ctctcttagc caggataagc
    16501 gagataagag tgaccagata aggtaaggca aggacaagca gaggcggtat tgcagagaaa
    16561 ccttgcagat agttcgatga gctttcggca aatgcaaaga gcaaacagga tgcagccacc
    16621 caaaaaggtc tttttcggcc aagatatata gccaccagtg cgatccatcc gcgccccgag
    16681 ataatgtttg gcacgaaggc agaaatagat aagcccaagg catatccgcc taatccgcta
    16741 cctatgccag accagatgag agcctgcata tgtatccgat caggagaaaa tccaagagcg
    16801 gagacagctt gagggttgga gcctgtagca cgaattctta gaccgtgcct tgtatttttt
    16861 attatgaaag cagaaatcaa aacccatagc cacgcacaaa tcgtgaacat tgaacgataa
    16921 gagaataaga cgccaattaa aggaagttga gatatcgagc ttgagaataa atgactatcg
    16981 attgggatgt cgaatgcgac gaccgctttt gtgtggtacc aggcttgaga caagactgct
    17041 gttataccct gagcaagcat attgattgct aaacccgtga taaagatatt cgttcggaac
    17101 ttactagtca ttaggcccat caacatcgag gcaagcgaag cggagaggat cgcggcaaga
    17161 gctcccaaaa agatgttatt tgtcaaaccc gcggctgcca tgccaaagaa ggctccgata
    17221 gccataagtc cttctagcgc tatatttagc ataccggaaa gctccgaaaa aagacctccg
    17281 attgaagcga aaagcaaggg cgttgccgcg gctattgtat atctcaagaa gtctatcaag
    17341 gccattagcg ataagttcat tattgtcttc gcctctgccc taatcccggc aatacctggg
    17401 ctgtgataaa tagcatcgca gcagcctgga gaatagaaat aatctccgaa ggaaccccag
    17461 atccaatcat tacatggtca gagcctgctt ttaaaaaggc aataaaaaga gccgagggca
    17521 gtattgcaag ctcgctgcct tgtgcaagga gagcaactga aattgcactc cagcccagcc
    17581 cggaagaaaa gccgcgcatg actcgacctt gtggcccgat cacaagaaga gcgcctgata
    17641 gcccatacag gccaccagag atggccatgg ctgcaatatt atatgctcga acggaaatgc
    17701 cacaatatcg tgcaaattcc tcattttctc cgcaaaccat caattcgaaa cctattttgg
    17761 atttcttaga taagatctgg aaaaacaaga taagaaagag agcaaaccat atagagatat
    17821 ctagtttgga aggggcaaag atccttggca aaagaaattc cgatggaatc tgggccgtag
    17881 tctgaaaatt acttgtcctg tcttgaagca aaccggtaat gaagaaatcg cttatattga
    17941 tgataattgc cgagagaaga taggatgaaa tcatctcaga gacctgccaa tatgctttta
    18001 gaagccccga gattgtgcca atcatggctc ctataaatgc cccgataaaa gcgactagaa
    18061 tccacactgc gctcggacca aatgcttctg aaatgtgcaa tgccagtact gctcctgcga
    18121 gagctcctgc ataaagctgg ccttctcccc ccagattgaa atttctagat ctaaaagcaa
    18181 tggatacacc aagacctgca attatcagag gcacagtatc ggcaatgtag tttccaaaaa
    18241 aataccggct ggagaagggt tccacgaaga actgccataa tgcagatcta aaatccgctg
    18301 caaacaacaa gagtactgtt gtcgcaagaa taatggaaag agttgctgct agaagaacct
    18361 taagagcagt agaagatgcc ccttgatttc tcattcatga atcctgttgc caagaaggta
    18421 ttcaccgata acggctctat tgagccaatt gccgttttct tgatacaaaa ctcgtctgcc
    18481 tcgggagagt accatgacat aatctgacaa ttcgagtatt tcatcgatat tcgaggatag
    18541 cagaagaatt gcagtccctc tttctctggc attgcgaatt ctcctgtaag tatgctcggc
    18601 cgagcgaata tccaggctcc aggtcggttc gcatagaata aatagcttgg gtaagtctcg
    18661 agagaaaagg cgtgcaatag acaatttttg aatattgcca ccggagagct ctcttgcagg
    18721 ctgatcgggg agagcattta tctcgaagga atgaatcgca tttacaatcg cttgttttgc
    18781 ggcattttct agaatccagc ctttgggaaa aaaatcctgt ctggagagag caataaaatt
    18841 atctcgtacg gaaagagaag gtgcaattcc tcttctaatg cgatccgatg gtagatacgc
    18901 aataagtctt ttttgaaata agctttgata ccttcgagaa ttatgaagct ctacattatg
    18961 accatcgaag gtacaagttc cagaagatgg agatcgaaat ccagcaagta cgtcctcgag
    19021 ttcgtcgagc ccattgccgc tcagcgcgct gatgcccagg atttcgccgg catgcacttc
    19081 gaaagatata tctgaaagcc ctactccata atgctgatgg cgacggacgt tcaatccatc
    19141 aatatgcaag actatagttt ttttttgagt attttttatt tgtggctttt ctataggctt
    19201 ttgtgtagcc tcgataaagc tctttcccat gataagggat gtgagctctt caagagtggt
    19261 ttcggagatg tcataggttc cgactgtctt tcctccgcgc attacggtta ccctatcggc
    19321 tatctgcatt gcctcatgga tcttgtgagt tacaagaata attgtgtgac ccaggcgctt
    19381 tatttcggat agtttttcaa atagtacctt tgtctcttgt tctgtgagaa cagaactggg
    19441 ttcgtcgaga atcaatatct tggtatggcg cgcaagctgc cgcagtattt cgatctgctg
    19501 acgagcagcg atgctcaagc aagcagctgg tgctgaagga tcgatagcaa agccatattc
    19561 tgtggcaagc tgcagggttc ttgcatttag ctcctgcgag tcaacaatgc ctgctatttt
    19621 cgaaacctta ttgtttcgat gtggttcgat gccaaaaaaa atgttctccg cggcactgag
    19681 ctcatcgaac atcaggaaat gttgatgaac catgccgatt ccaagggctt gagccttgct
    19741 aggcgattcg atctttacga attttccttc tatttcgata tttccttcat cgggttgatc
    19801 aaggccagcc aggattttca ttagggtagt cttgcctgct ccattctctc caacgatggc
    19861 gtgaatacta cctttttcga cttcgagaga tgcgccatca tttgcatgaa ctcctctagg
    19921 gtaatagcgc ctgtatattc cttgcatcct taccgcgagc atagcgagca tattacagtg
    19981 aaggaacctg aaaggtaagc cgacccgatt tgatcgattg gattaccgca tcgactttta
    20041 tgcggataga ttccggtaca tttttgatat aagaaggatt cttgttcaag aattctatgt
    20101 agccttcttt catgcctact atttcggcct ttccaaattg cagtttttct tcgattgctg
    20161 ccttcaagct gcggtaagca agctcctctt gatgcagaac agtgcatcca aggatattgt
    20221 caggagcccg tgcaaattca tcgtcatcga agaagacgag atacttgcca gtctcttgag
    20281 caactctgac cgcgccctgg ctcgccgaac cacagattgg cagtatgata tctgcaccct
    20341 gagcgtatag actgcggctc agttccgctg cctttgctgc atcgtaccag tttccgagca
    20401 cacggatatc cagctgaaaa cctggatcga cagccatcag tcctttcaga aaacccggaa
    20461 tgatcatctt ttccattgcc ggataattct gacctattat catgccgatt tttttttctg
    20521 ggtttgttcc cttcatgcca gatatcgaga caagccctga gagatatccg gtgacatagc
    20581 cttgttcgag ttgattgtat agtgctgaat agacactgga attgcccgca agatagccat
    20641 cgaggcaaat gaacctttgt ttaggaaaga tcttggaagc ctcatttacc agctctggca
    20701 tcgaaggatt ggaggtgatg acgatatcga atttgccaga ggcgacaaat gagatgagtt
    20761 tttctttcca ttcggcttga ttaaaacctg cttctgtgac tcttaggctt gctctaggcg
    20821 tttcagagac aagcctttcc gctccttttg ccatattatc gtagatggga cttccgtcgc
    20881 gagcgccagg aacgaaaacg cctattctag gggcttgtgc ccatagtgcg gtactcgtca
    20941 gaatagcaac cagagatgcg attataaatc ggcgcatggg attcctcctg ctataccctg
    21001 gagggcagga aatatgatag aatcttacat agcttaaaat ctaacgtgag ataaaatctc
    21061 acgttagatt tagtatagcg atcttaataa tatgttcaag gggtgtacac attgccggat
    21121 aagcttctaa agatcggtga acttgcctcg ctatttagag tcacggcgcg aaccattcgc
    21181 tactatgaag aactcggtct catagaagca agcaatcgca ccgaaggatt ccatagacgc
    21241 tatccagccg atgccattat ccgattgaag aggatagaag agcttaaagc gatgggtctc
    21301 actcttggcc aaattcgcga gtttttcatc ctatatagcc aggatccaag tggcgaaaaa
    21361 tgccgacttc ttcttctaca gaactacgaa gaccaaaaaa aggaagaaga aactagaatc
    21421 cttgaggcac agcgccgtat tgctcaaatc gaagcgaaca tcatagcgat aaaagaaaag
    21481 aagtctttct tctcttgccc tggtgaggag tgcaaagtgt gcaatttcaa taccttctgt
    21541 attgattcag agcataatcc tgacaaggaa atgagatgat gaaaaaacca gctattgttg
    21601 ttctatgcat ttctagctat gttgtggtaa actcagccct ccatgccccc tttattgtaa
    21661 aggatctttc atccagcaag ttaaggaaat gcatataaag ggcttgctta ttaatggtat
    21721 tccataggag ttgaattttg attcttgctg tcgatatcgg taccactcga atcaaggctg
    21781 cgcttttcga tgcggaaggt agaagcttca aattagaaat agtagatctt ccagatgaaa
    21841 gttctaatta ctatgaggaa ataaatgcgc gcctttggct taaggcattc tctgagatca
    21901 tctctcgttt gatagcgacg ggagaaaaga atgaactcaa agctattgtc atatgtggca
    21961 atgggcctac tattcttcct attgatgaaa gaggcgagcc gcttgccccg gcgatcacat
    22021 ggctggatag gcgagcccaa caagaggcca aagaggcatc tattgcacta aattattctc
    22081 tcgacgctgc ctttaatctt cccaagatcc tatggatgag gcagcatttg ccgaatatat
    22141 acaatagagc acatcttttt gtatcctgcc cggaattcat cgcggggcgt ctcagtggtg
    22201 aatggacaac ctgtcttccc aatcagggct atacgcggat tatctgggat acaaaaactc
    22261 ttgaaatgct caggctggat atcgagaaat ttcctcgctt cgtaggtgtt ggtgagatag
    22321 taggatatat tcatgatcat gctgcaaggg aatttggtct tcctaatggc ttgccagtcg
    22381 ttatgggagg ccctgatttt atagcctcgc tgctgggtac agcgacagtt aagccgggaa
    22441 ggacctgcga caaagctgga acttccgaag gcatcaatct ttgttcggaa agagatatcg
    22501 gagatgaaga agcgcttctt gttatgcctc atataattcc tccatatttc aatatttcgg
    22561 gagtaatatc gtcaagcgga atagctcttt cgtggtttaa aagcaggttt ttgcctcaag
    22621 aggattttga gacgatctat gcgcggattg cctcagcaga acccggagcc gaagggcttg
    22681 tgtttcttcc ttatcttgca ggcgaacgat cgcctcattg ggaccccaat gcgcgagccg
    22741 tattcctggg gctggggctt aatcacgatc tcagcacgat ggcgagagcg gtgctcgaag
    22801 gcacggcttt tgctatccga gaagtgctag atgtaatgga aagcagagga gccaccgtgc
    22861 atgatatgcg agctacaggc acaccttcgc tttctcccat atggaatcag atcaaggcag
    22921 atattacagg aaaacagctt atggtatcct ccttcccgga accggagtta gcgggctgcc
    22981 ttgctattgg tcgattcgct ttgggtgaag agtctaccct cgctgaagcc gcagaaaagg
    23041 tttttatgcc agccgaaata tttgaacctc gaaaagaaac agaatccatg tactgcgaat
    23101 tatatgaagt ctatctggat agttatcggc gacttgcaga cctctttcct accatttcgg
    23161 tgcttcagag gaataatggt gttcaacgag ggcgttttgg tactggtttt tgagaaaggc
    23221 ttttcaaggc aaatcggcca taaaaaatta tcgtctctgt tcctttgctg caaataatat
    23281 tcctattctt gcgacaaaat ctcttctggg ataggatagt ttactgtgct tttgagaaga
    23341 caatatcatg gccacattca gtatatcgct ttttgccagg aggattaaat gccaaagatg
    23401 gagatagtca tcaatctatc gaatcaccat atccatgtaa accacgaaga tctcgaggtg
    23461 ctttttggaa aaggtcatga gcttacaaag accaaggacc tcattcagcc aggtcagttt
    23521 gcttgtgcgg agactgtcac gataagaggg ccaaagggac agctcgaagg ggttcgaatt
    23581 ctcggccccg agcgtaaaga aactcagtgc gaaatcctgg cgagcgatgt ctttaaacta
    23641 ggcgtgcaag gctgccctgt atgtgaatca ggtcagctcg aaggcagttt cccctttgag
    23701 gtaattggtc cagctggctc gataaaaaag gaaagaggtc taatcattgc aaagcggcat
    23761 atccatttcg atttggaagc ggccaagcat tttggagtaa gtgacaagca gatcgtcaag
    23821 cttaaagcag gagaggaacg ccgagctatt cttctaaatg tcgtatgcag ggtccatcct
    23881 acttatgctc tagaatgcca tatcgatttc gacgaaggta atgctcttgg cattacgagc
    23941 ggagccatag gcgaaatcgt ctattgttga actaatgaac gaatcaagcg cgatgggagc
    24001 atgagcctta agattggagt accgtgtgaa tagatttatt ctaaaagcta tggatctcga
    24061 tggttacctt tcgcagagcg atcttgaata cctttcgcgg atgaatgagc tctatgagaa
    24121 ggcgctggtt tctttcaata tcctatccac cactacctcg gaagcgcaga gacatcatga
    24181 agagaatttc ttgatagagc tctacaatca aatgggcaaa ttgatgcaac agatctgtgc
    24241 atcggagcca cggattcaag tctattcatt cgtgtcgcca caggaaacac atggggaagt
    24301 atcgcgcttg attgccaagc tgcgagatgt gaataccgga catcaggagt tcgtctacta
    24361 tatccagcga gcctttgagc ttctgttcaa cctggccttt ggcggagcag gcaagacgaa
    24421 taaaaactac cttatcgtgc gcacgcccgt tactattcct gtacagaatt atgccgtcca
    24481 taaaataccc aatgttgata gcgccgtaca caatacggtc atgtgcgtca tgttgagggg
    24541 tgctctactt ccatcgatga tactctccaa ggaaattcag gagtattctt caactggtta
    24601 ccttactcct tttgcgcttt tcaagataaa gcgagatgag agcaagagcg aaaccactat
    24661 ggaatatgtg ctggaccttg accattcata ttttgaactt tctgagctgg atggtaagca
    24721 tctgctcttt gcggatccca tgaatgcaac aggcggttct cttgtcacag ttgtgaaata
    24781 tcttctcgaa cagggagtaa aaccggcttc tatcaagttt ttcaatgtta taagcgcgct
    24841 gaaagggact ctccgcgtaa ttcgagccat cgataacatt atgatataca ctctatggat
    24901 ggatccaagc ctcaacgatc gtgcctatat catgccgggg ctgggcgatg ccggggaccg
    24961 tatcaacggc agcgatgaag atcttcatcc gcgagatatg ctgagactcg ttgcagatta
    25021 tggaaccaac atcacaggat tgtaccgttc ccagcttcga ataatcgaag agacggtgct
    25081 gaggcacaga agatgaccat attcccggag agttttgtcg tctttcaagg acaagactgc
    25141 cttatacctc cgcaagatgc ctataatatg cgtttcctgg atgagccttc gctcgctgca
    25201 tcgataaaac ccgaagccgt ctgctggcac ttttctcaga taccatctga tctattatct
    25261 ccttcatcgg atcactatgc ctattcggtg tttgaatttg atgacaggaa atatggggca
    25321 attcagatcc aggataatga tgatattttg atcaagcgac caggtctaga cgaagccatt
    25381 gcatttcctg tgcgtgagct tgttgtttcc tatccttcaa ggttcagtcg ttttatgctc
    25441 attgctaaag cacatggtca atgggcatcg gtctcccgat tctgcggagc ctgcgggagt
    25501 cttcttgtcg atgcaaaaga aaacgacgag gttagagagc ggctcgatga taatgcatat
    25561 gccgcaagat tctgcccgcg ctgcaagagg attttctttc ctcgaatgtc gcctgcggtc
    25621 atagtgcttg tgtacagaga gaattcaatt ctgttggggc ataatgtgcg ctttcctcgc
    25681 aataggcata gcctaattgc ggggttcgtg gagattggtg agacgcttga agaaacagcc
    25741 attcgagaaa tacgcgaaga agcaggaata gaagtgaaaa agctgcatta tgtgctttct
    25801 caaccatggc cattccccga ttctctaatg attggattca ttgcagaatg ggcatctggc
    25861 gaagcacgac ctgacaggaa tgaaatcgat catatcgcct ggtatggagc agatgatctg
    25921 ccagagcttc cattgagagg aagtatcgct cgttatttga ttgacagctt catagccgga
    25981 ggtttttcta atggctgaac tctgttgtat ctaattttgc atgtaattga ttctatactg
    26041 gatagatagg agcaaatcat ggatttcagc tggaaaatcg ttatcgatgc agggcttatt
    26101 tcctttgccc tggtgattgc cacttttttg aggtctaagg ttcgatttct gcagaaattt
    26161 cttgtgccaa atgctctgac agccggtttt ctgcttttgc ccttgtataa cttccttatg
    26221 ccagccatag gttatgggac gaatagactt ggagatcttg tctatcattt gctcaacctc
    26281 tcttttataa gcatgtctct ccgttcttcg ccacccaaga tcaaaggttc tcgttccaat
    26341 ggaagcgtgc tgggtatgtc agcggtcatt cttcttggat atgcttcaca aggacttctt
    26401 ggcctcgttc taactctatt ctttttgcct aatattcacc ctgcatttgg attgcatctt
    26461 ccactgggtt ttgcccttgg acctgggcag gcttatgcca tagggaaagg ttgggagtca
    26521 atgggttttg agggaggagc aaccgttggg cttacctttg cggcaattgg ttacctctgg
    26581 gcatgtttcg gaggaatgct cctgatccac aagggcttgc gcttaggctg gatgaagcca
    26641 gagcaggtaa gcatcatgtc tgataaggct ttgctgactg gtatagttcc aaatggtacc
    26701 gaaaaaccag taggagagcg gcttaccacg gattctgagg ccatcgattc ctttagtttt
    26761 cattttgccg ttgtggtgtt tatctatttg ctcagctatc ttgccttaaa aggaatatct
    26821 ttcctgcttg gattcgtcgg ggaagctggt acggagcttg cagccaatct ttggggtatc
    26881 aattttatct tttctgctat tatagccata attgtcagga aaattatgga cctgctcaaa
    26941 gtgggatatg tgctcgacga cgatacccta tccaggattt cgggtacttc cgtggattat
    27001 atggttgctg gtgcgcttgg ggctatttca ctcgtatttg tcggtaaata ttggtttccg
    27061 attctgctta tgtccacatt atgcggattt atggtgtact acacgattcc atgggtgagt
    27121 tcgcggatat ttcgagattt tagatttgag cgcatgctta tgctctatgg ggtttctact
    27181 ggcacactct ctactggctt ggctctttta cgggttatgg accccgaatt taagacaaag
    27241 gtaacctcag actatatgct ctctgcaggc ttgacctttg ttctggctat cccattcatt
    27301 cttgcgatca atctgccggc aaaggcttat acgagcgggt ctatggtttc atattggatt
    27361 ttcatggcta tagccgcagg ctatatgctt tttgtgttgg tagtgtattt gattctagcg
    27421 agaaaaaggc gatttgcctc gcccagtgct aatttttatc gcccgggcaa atgagatttt
    27481 gaaatttgat gcagtacgct gatggcgtcg gcatattttc tgcctgatgc tcttttgtct
    27541 ttgaagacga ctcgatagac attcatgccg tgatagaact cgaaataatt ccacttgagc
    27601 acgccttctc catcgatttg ctctatagga aaaacaattg gatcgagttt ggtatttctt
    27661 gtgttatgga attcgagtcg gtctctatag agttcgatac ggccattgag caaggtctcc
    27721 atatgatcca tgcgtttgcc gcgctttagt ttgattggtt cgtcaagaaa gaatggagct
    27781 tcaagatatg cacttttcaa gccagagaga tattgatata gaagcctact ctgtaaacta
    27841 tcccaactta cgattgagtc gagaaaagga gcttcaacga acggtggtat ataatcttcg
    27901 cgatcccctt gcgaatgtag caagaatgaa ccatcgtgtt ggtattccac ggcaaagcca
    27961 cattgtttgc atgtgaacag attgccttga ctgtgcattg aagctctctc tccacaacga
    28021 gggcatagat agattgccaa ttcaaggctc tcggcgcctt ttgtgctttt gaattcaatg
    28081 cctttgcgct tacaccaggc tgaatcatca taggaaagag cttcgttgag taatcgatct
    28141 acagtggaga gtctcattgc tcctagctgt tccgggctaa acagaagcga aaacgagatc
    28201 tcgacttttc cttgtcgtag aacatgagac caccggggtt ttgtaagata tccgccattt
    28261 gtctttacca atactactgg gactcgcaat aatcgcagca acttagcact ggaactatat
    28321 acaggaaaag aagttcctgt ccaagtactc tgaccttcgg ggtacattcc cactacacca
    28381 tgcttatgac ttatcaaatc aacgatccaa ttcaccgtct cgatatctgg tatggctttt
    28441 gatttcggaa tacttcctac tagtttgatc agcataaaac gaatgatcgg atttctcata
    28501 ttgccatcgc tagcaaccca gtgtacggga aaaggcacga atgcgttggc aatgaaggga
    28561 tcaagaattg ttgtatgatt gcctacgaga aggaatggcg gcttgagttc cttaaagagc
    28621 tgggtattta ttgctgtgat ccgatatctc aatttgagcc aatttccata ggtaaatctc
    28681 aaaagccctg taaaaaatct aaaatttgtc ggagtgtatc gtcggttgag aatcatgaat
    28741 gctaatccct ttcatgattc taatgttcga atttgaatat agcaagacgg gtcataagaa
    28801 tggcgttgct cgctttttgg attcactaga aatcctcata tcagcccaat gaaataaaca
    28861 taataatgtg ctatgtatac attcgaagag gaacctaaat gaaacaatag attacgagga
    28921 gacgtctatg ccttgtaaaa agcccctgtc atcgtccaat agcgaccaaa agataagcga
    28981 gaatagcgta tctcagacct tagagcgatg gctcgaagtt catgagcccg tcgacaaaga
    29041 taagactaat gaggatttag aagcggataa aagactggat tcgatttccg cctactcaag
    29101 atttaagcgt atacttcctc aagatgaatt agatcttcac ggattatatg cggaagaagc
    29161 tcgccagagg attcaagcct ttatagccga tagcgcctca agaggcctgg aaaaagtgag
    29221 cattatacat ggaaagggga atcattcgca tgaagagcag gttctaataa atatagtccg
    29281 ggaagagctg gagaagaatc cgcagacagg agaatttgat tttgctgaca gaaaacatgg
    29341 tggcaggggt gctacctggg tccgcattcg acctcctatt tctcgcgata gataattcgt
    29401 cctcgattca aatcgtatgg tgagaggcca acttttactt tatctcctgg cacaatcctg
    29461 atatagtgct tgcgcatttt cccggagaga tgcgcaagaa taatgtgtcc attcgccaat
    29521 tctacgcgaa acatcgtgtt tggaagagct tcttttacaa tgccttctac ctcaatagct
    29581 tcttctttag ccacatatac tccttgatgg taaagtctta ggacgtctat ccatcattga
    29641 tggaagctac atccgtcctt tgcagaacca tgttatgcac aaaagccaca aaagaaaaat
    29701 ccttgcggcc acaatgcgaa tgaaatagta tgaatcaatg catagaatgt caacatgagg
    29761 acatctctcc acatgtattg acattttcgc caaagtgatg cattgtgccc atccactgca
    29821 tactggtttt ttaccagcaa ctgatgaggg ggagagagat gggaaatgcc ttcatcgata
    29881 gaatgaagaa cctcttgctg gctcagagac aagaaatttt gaataccatt gctgagtcga
    29941 ataaagagct cagaaaagcc ctttctgaga ccgatcccaa ggattcggcg gatatcgcat
    30001 ctgacgacat cgataggata atgatcgaag ccttgagttc tcaagatctc aagcgctacc
    30061 aagcgataga atctgcactc cttcgaatca atcagggtcg ctatggtctt tgcgcaaagt
    30121 gtggcaagaa gattccggag gagcgtctag aggctattcc ttatgcggtg ctttgcgtcg
    30181 agtgtcaaaa aagcgacgag cgaaagaacc gttgatctat ctaagctggg ctttgactga
    30241 ctctaaggtg acttctttcc agacacctgg agcatccata taccacagcg tcttatagcc
    30301 ggccatctcg gcgatatcca tggcttttgg ctgatagacc ccaatatggg aaggtgtatg
    30361 ggcatcatct ccaatggtaa ttcttatgcc tttctcttgc attcttttta gaatgaagag
    30421 tgacggatat ggctctccgc gcttgctccg cgcaatacct ccggtattta tttctgcgat
    30481 atagtctttt tcggcggcta agtccacagc ctggaatgcg gcatctgtat aggctcgatt
    30541 ctcttcatca aaccagcgtc cagctatatt gtttttcttt actaagtcaa aatgccctat
    30601 tatatcgaat ccgccggctt ctatcatttc ggaaataaag caccaatatc gtttccaaat
    30661 ttttcgataa tctccatctg gcacccaatc ttggacatgt agagcgaact gatcttccgg
    30721 ttcatcaaca gtaaatggat attcgcctgg ggtagtaaca tagtgaactg aacctatgcg
    30781 gtaatctagt tgcatgggct cgtaagccgg gtcatcgggc tttgctattc ccggcacgta
    30841 ttcggtctcc agacccaaga agatcttcat accctttggc tcatattcct gagctaaaga
    30901 acggatagcg tagacatagg aatcggcctc atcccagggc aaattacaat ctgtctcaag
    30961 ggagatcggt gcgtgggcgg aaaatccgag atgggtgtaa tgatgttcga atgcggtgcg
    31021 agccattgtg gttgcatctg cctttccatc gcaaaacagt gtatgagtat gaaaactgaa
    31081 ggccataccg gatactagca tttttctacc ttttcttcta tgcactatgt gggtgtacga
    31141 aaattaaatg taaatgcaat catgctgaag tttcgtctat tgacgttgta aaagcccttt
    31201 cttactatga tagaatgcga gaatcttctg cgagatctat tggagtcttt ctctcggaaa
    31261 ttagcaaagg agaaaaactc gctaggtcgc tcagagaatg tggcgcttct gtcgcgatcc
    31321 tgaatggata ttcggaagat ctttcacgac aattcgattg tatcgtcgcg gagattggac
    31381 tgctcaatga aagagaaatc caagctattc tagacagctc gactgccttg gtgttaatcg
    31441 aaaaagctgt ggaagagttg catagaactg aagatgccga atattcttta gggcaattct
    31501 cgaactctgc acttctaagg attttgccca gaaatgcaac cagctcgaga atcaaatgta
    31561 ctatctatca gctatttgca aagaaggata gtttactctc gtctaaaaac actatagcgg
    31621 ccgcgcaaag ccagttgcgg acactgctat cctctcttcc ggatattgta tatgtactgg
    31681 atgaacaggg ctattttctt tatctaaatg aatcgattgg tctgttgggc tatctgcctt
    31741 cggagttggt tggaaagcat ttcggtacaa ttattcatcc ggaagacaga ccaaatatct
    31801 cgcgagatct tgttgttgag aaaataaggc aagacaatat aatgcccgca aatcctccaa
    31861 aactcttcga tgagaggaga tcgggttcgc gcatgacaag agaactggaa gtacgacttc
    31921 tgcataaaga tggacatgta atctatgcaa tggtcaatgc ttatggagaa aaggccttcg
    31981 atgttccgct tttctcggat gtggttggca aaaagcttca gaccataggg gtaattcacg
    32041 acatcagcgc aatgcatctc tatcaagaaa gcctggagga atctctggct gcaaaagaga
    32101 ggcttcttcg cgaaattcat catagaataa aatcaaatct acagttggtg gctagcttgg
    32161 ctcacttaaa gcagatcgat gctcaatctt cggcggctat cgaggttttg agagatctag
    32221 aaacgcagat aaagagcatt gcgcttgtcc atgaagctct ttatcaatcg gagagaatcg
    32281 atcaattgtc tgcgagggac ttctttggac aattctgtca ttttgcagaa gaagctctgg
    32341 agagggtagg gtccacggta caaatcagag tgtccgccga agattatcct ctggatccga
    32401 atctccttgt tccgctttcg cttgccttgc tttatctcct ggaaggcgct tataaggtta
    32461 gctatgatag acacgaaaag gtggaggttt cgctttcttt tatgcgcgga caagatggca
    32521 tgcagatcat tgagatgaat ggagaaggga ttctagagaa cgcaaattcc attgtcatgc
    32581 aagctctgtt gagacaagcc catacaagct tgataatacc caatacagag aagggtaggc
    32641 cttcttgcca tctaattgag gaagcaaagt cttaaatgtg tatcaggtaa tatataaaaa
    32701 tcattatatt aaaggcatgg aggagaagtt gatgcatcag atttatgatt ttgcagttat
    32761 tggagcaggc gcagcagggc tctccgccgc tcagtatgga gcccgtgcca acctaaatac
    32821 aattgtaatc gaagaactag cccctggagg tcaggctctt tttatagata aactggaaaa
    32881 ctatcctggt attctagagc ccatcaatgg ccttgaactt gccgagcgta tgcaaaggca
    32941 ggctgagcaa ttcggcagct cttttttcga agcgagcgca ttatcagtgc gcaaggagaa
    33001 aggccacttc ataatcgaga cgagtgctgg acctctggag agtctcacag ttctcatagc
    33061 tacaggcgca agacatcgac ggcttgatat tcctggagag caggaattcg taggcaaggg
    33121 tgtttcttat tgtgctactt gcgatgggcc atttttcaag gggaagcgta ttcttgtggt
    33181 gggtgggggc gattctgcct gtgatgaggc tatctatcta tcaaaacttt ccgacaaaat
    33241 tgtcatgata catcgtagag atcgctttag agcacaaaag gctctctcca gccgagttct
    33301 ctccaaccct tcaattagag tcctcttcaa caccgttgcc aaagagatca aaggcgactc
    33361 gaaggtccgt tctgttgtcc tggaaaacac catttcgcat gaattgaccg aagagccctt
    33421 cgatggaata ttcatattcg ttggttccac acctcaaacc gcctttctca aagatatagg
    33481 tgtgcagttt gacgaatcgg gttatatcct gactaatcct aacatgcaga caaacattgc
    33541 aggtttattt gccgcaggcg atgttagagc aactccattc agacaaattg tcactgcggc
    33601 cgcggatggt gcaatcgcag cccgatctgc ctcagactat atcgacgagc aaagagggca
    33661 gacttatcga tgaatcgatg agaggtatgc atgaaaaaat cgatgaattc ttgtacgctt
    33721 gattctgtgc tcagaattcc gattgaactc gtatgtggtg tagatgaagc aggaagaggt
    33781 ccacttgcag gtcctgttgc cgcagctgct gttattttgc ctaaaggctt tcccattgca
    33841 atactcgatg actctaagag gatgagtgct cgccgccgtg aggaggctta ttgcattatc
    33901 gtagaacaag cgctggactg ggcagtggga tgggctaccg tagaagaaat cgagaagcac
    33961 aatattctga tggcgagtct gcgtgccatg gaaagggctt ttggggggct atctcatctt
    34021 ccagcgctcg ttgttgtcga tggtttgttt gctccatctc tgagcatgaa tgggacaaat
    34081 atagaagcgg ctaccatggc taaagcggat ggccttatcc ctgctgtaat ggccgcttcc
    34141 atactcgcaa aagtggctag agatcgcctg atggattgtc tcgattgcat tatgcctgag
    34201 tatgggttca gaaaaaacaa aggatatcca actaaggcgc atcgagctgc catagagcgc
    34261 tttggtccat cgcgatttca tcgaaaaggc tttcaattat tgattcaatc cgatgcgccg
    34321 cttttcgatt ctctggaatc tcccgcagaa tgaggtttct ttttaataac aaatcgacga
    34381 ggagctcgcg atgaactctc atgtaacgcc tgatgagtct gtcggggctc ttgcctggtt
    34441 cgagaagact gtatagccgc tctatccggt ttgggccttt gactggcttt ttccatatag
    34501 ccagccgcgg ttctcaatgc tcgcatgaag ccttccatgt tttcctggaa tattacgatt
    34561 gagcgcctat cgaaggtttc tgtctcagta ggcttgctct ccacaattga cagaaaaatg
    34621 tcaccattcc tattttcttt tacgttaaaa aaataagtcc ttccttcaca gatgaacctt
    34681 gtagagaaca gttcgccgcg aatacccatc ggaactcctt ttgtatgcgc ctatcctaca
    34741 ttattttgtc tgggcgcggc aaggagatcg gcctcggtga ccaatcctgc atgccatctg
    34801 cgtagccaag cttcgtctga acatgtcccg tttttgatat ctacctcaat gcggaataca
    34861 ggttctgtgc ccgaagctcg catccagagg aacgccctgg gttcgtccgc cttatcgtag
    34921 agtacgattc gaagtcctcc attctttgag gagccaaaat ctgattcaac ctcaaattca
    34981 tcaggcccga gagatgcaaa tgctctccat gagacaatgt cgaatctttg ctcaagttcg
    35041 ggtagcattt taggccattc ctccaggaaa agtctttggt aggccttctt gagttcgatt
    35101 ttatctacgg catgtatttt tagcgcagca tggggctcga aggcgctcgt tgtaatccat
    35161 tgtggcaggc tttctatgac atcttcgaga ttatagccag actggtagcg ctcgggacta
    35221 tcaattgcct ctagccagag attgaaacag gtttcttttt tctctgcgtc gccaagcctt
    35281 agtaagcgaa ttatagatcc aatagtggat aaaggatcac gcacttttga aggatgagtt
    35341 atattgcctc cattagagcc ttcgccaaga atgcggactg tccatccttc tgctctgagc
    35401 ttttctgcgc ggcagacaac attggcctcg cctgtctcgg tcctgaagac ctttgctccg
    35461 aatactctgg ctatggcttc aatgcgcata ctcgtcgcat cgttgactac gattgcgatt
    35521 ctgtttttct cgcctttcca ttgcatgtac gctagctcgg ataggcaggc caaggcaaag
    35581 acctgttgcg cttccagagg aatcgcctct ccaagtctat tgccataata cacaagattg
    35641 cctcgatctc catcgcaatc cggaacatag ccaagagcat aggctgcatt ctcattgtga
    35701 gccaactgca aagcctttct gcaatcatcg agcgattcgt tttcgggtac gattctatgg
    35761 gcaaagacgc caggttgaat gttcaaagcc cttgtctgaa ctccgattcc ggagagccaa
    35821 tccaaatcga tgctctgtgt ccgtgctgat ccattgagct ctgcaattat gccaaaaggt
    35881 cgcttttcgc aagcctcggc gatctcaagg agtattcttt cttgagcttc ggctgtctct
    35941 tcgtcagtga agactcgatg ggcaaagagc atgtaagccg attgcgaata acgtttccaa
    36001 tgggcaacat tttcatagca ttttgtcaag gcccgagagt cagccttttc aagtgccata
    36061 agtgcaatct ctgaggattc gggatcagat agaaaaagct taagatctga gataagcggt
    36121 cttatctgtt cgccagtcag cacacctccg ccaaggccaa atttgatacc attatacccc
    36181 ggaggattgt ggctagcgga gacatagaca aatccatccg agtgaaattc attatctaca
    36241 tctaaagtcg taacatgagc cgcgtatgcc gtgatttctg gaattggaac tatgaacaga
    36301 tagcgaacct catactcttc tccaataagt acgcgagcaa agatatccgc aatagcgggg
    36361 ctcgtaggcc ttgtgtcgat accgagcaga atgctgggtt tgtggggttt acaatgttct
    36421 tttatgaacg aagaaaagac caaggccatt cctgccacta ttaccatatc tgttggggct
    36481 agaagaggtg aaagagagtt gtccggcaat tgttcataat cccaggaggc atagcccgca
    36541 gcgagctcgg gcttagcgaa gatcttgcgc catcccgaag gtgagaggat tagttcttgg
    36601 gtggctagtc tgatatcttc aaaggaggga agctcttctc tagatagagg tatcttcccc
    36661 gggtccccaa taggcagacc caaaaaaggg tgagtgatca atgacatgct ttgctcccag
    36721 caatattgta gctacttttc tcgattctga gaatgatgat gctattgatc gaaatcgctg
    36781 cattagggca aaaacaagag caggcttctg tctgtggcaa ctcagcaaaa gtgcttattt
    36841 acctcaattg tttctagcga tatat
//
LOCUS       DAOOWY010000008        36283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1201_length_36283_cov_11.020150, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000008 DAOOWY010000000
VERSION     DAOOWY010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 36283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36283
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1201_length_36283_cov_11.020150"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PK105_01400"
     CDS             complement(<1..504)
                     /locus_tag="PK105_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU60390.1"
                     /translation="MKRTLLIAMLFAVVAGFVFAETQVGIVLPTKDEPRWIQDQTQFL
                     DALKTAGFSAKVLFSQGDSAKEKANVEALISEGIKVLIICPHDGTAAASAADLAHSAG
                     VKVISYDRLIRDTTSVDYYVTFDSFEVGKAWGDYLNSKVPAGTKRNNLYLYAGAASDN
                     NSFIFFEG"
     gene            complement(745..1800)
                     /locus_tag="PK105_01405"
     CDS             complement(745..1800)
                     /locus_tag="PK105_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU60391.1"
                     /translation="MPEAMRKASGPIFFLIAMILATTALGLLSCSKSEKTSETKSPLI
                     GFSLDSLVVERWRRDVDSFTKAAHDLGAEVVLRVANQNAETQIAQVRELLSQGIDVLV
                     IIPNDAERLSEVCREAKRKGVQVLSYDRLVHRANVDLYISFDNEKVGSLQAQAVVEVV
                     PQGNYVIINGATTDNNAFMINKGFHSVLDPFIESGKIQLMEEIWPSDWMSDEVRTQFE
                     ALVKPGQKIDAVLCGNDMLAETVISVLSENRMVGKTKVTGQDAELSACQRIAEGSQFA
                     TVYKPIDSLALKAAGFAVMMAKGEKIGYENKIEDGTYKVPYIALEPVLVTAKTLESTV
                     IEDGFHSRDDVYRNVKH"
     gene            complement(1793..3433)
                     /locus_tag="PK105_01410"
     CDS             complement(1793..3433)
                     /locus_tag="PK105_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU60392.1"
                     /translation="MKRVLIADDEIPIVNGLQLLFKRYFQDGYLVVGTAQSGREVIEK
                     SKTLAPDIVLMDVHMPGINGLDAIRELSQQGGARAFILVTAYERFDIAREALSMGVCD
                     YLLKPVSKERLEIALQAASSYLDRLHILEEKELEFKERQQTLLPFIKTAFFVYLRQQR
                     EAKDKEPRDPKRTKALKEELNLYKEVLQISGETGVMGIASFSPADGLANSLYEHFCST
                     LQYKTLALAGPLEDNRYCAWFLPLKSGIDQGTVAEIEITAFLELLGSSFSSQLATGEL
                     RFACGQPELLENIGRSWDNAVRNFGLGLESINRYRPRLGEDIAENREEPASVFDTQFY
                     DAIMEGQYAMAGQLLEKLILTVEETQDKPRDLYFRIIAALSFWSSKLAGGGVLSDNVY
                     RDFMDFGDIEKLWEERFWHLFASAVRGRFQSLQQHAAEVGTHSPFVVKALQYIENHYQ
                     EPITLESASAAIGISAGHLTRLMSDELKRGFARTLIGYRMQKAKELLKMPNISIREVS
                     QRCGYSDANYFARLFRRLTGLTPREYAARVTKGEDSDA"
     gene            complement(3430..4941)
                     /locus_tag="PK105_01415"
     CDS             complement(3430..4941)
                     /locus_tag="PK105_01415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOU60393.1"
                     /translation="MIDFTWLSRPLRARRSRTGLRDRIIANSAVILLVLVIATSYTAS
                     TSFNLARSVELLFHNSVSMQELRTVLRATQENLMGYLSAKNSESLREFIRNSTLLSNM
                     AHKLNKENKNDDVFLLEKDLAYLVESYLASAERAVQAKRGRDVTEYVSYYEEARRGAS
                     YIEFLSTRMDTIYLGQSLTGFSSYRANISLAIMSNGVLLLAAFLLSIALIARYSYTIT
                     EPLARLSVAAGAVGRGEYNHPLPPYERKDEIRTLHDAFVNMQDSVQKAFIELTRKAEL
                     ERSLMEERMRVLTYQHRLKDAELLALQTQINPHFLYNTLAAGWQIALSEGNEKTAEFL
                     EKLAAFIRYSLKPSTRFVLVSEEIECVRQYIWLLRLRFGERFEFCLDIQDEALGCETP
                     ALVLQPLVENAIVHGLKDIEEGGRVLISAKLEGDTIHFSVSDTGCGMSNECIALALAA
                     ASPEDTRPQNGIGLHNVVRRVALATGGQGRVEIESELGNGTCVTILLPAGKIS"
     gene            complement(4938..5957)
                     /locus_tag="PK105_01420"
     CDS             complement(4938..5957)
                     /locus_tag="PK105_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU60394.1"
                     /translation="MKHGAWRVLPIVVFAIALVGALAFNVALVSRSRNALASTILRNP
                     STIHSARFHIIAILPDTVDPFFMHLKEGLSEEAEEQDAALQIFYFSPTGAADSGGISG
                     EVLRWFEIALRSKTDGIILFKTAGLDVERLMEAAESAQIPFVPLAMDVSQEWINIGVT
                     GDSASQGREASALALGMLGAAARIGIILSSDTSLGYAFYEEPFYRGVEETLKSKQGAA
                     IVAAEREEESILGGEDACARMLAEHPDINAILCIDAKATIGAAQVVIDRGMVGQIVII
                     GADENSEVNRLIEKGVVQASIVRDAVAMGKAGVALAIGQRIGIRAPERISVGYRVIPA
                     LGGGL"
     gene            complement(5954..7786)
                     /gene="glmS"
                     /locus_tag="PK105_01425"
     CDS             complement(5954..7786)
                     /gene="glmS"
                     /locus_tag="PK105_01425"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOU60395.1"
                     /translation="MCGIIGYSGPRKASKVLLEGLRRLEYRGYDSAGIAVGRTDPKPN
                     LQIIKAVGKIGELAKKIPEDLDGQWGIGHTRWATHGGVTEANAHPHTDISGKIAVVHN
                     GIIENHKTLRTVLEKKGYVFKSETDTEVIPHLIASYYEGDLLTAVLAALQHLEGTYGI
                     ACIHADEPGRIVGARNGSPLIVGVGKDEMFLASDFTAMVAYTNRVIYLNDGEVVDITQ
                     ENYSITDRHSNSIAKKVDEITWELGAMEKSGFMHYMEKEIFEQPDSIARAMSGRIDEE
                     NATAKLGGLNLTRRQLAEVHRVRIIAAGTSWHAGIAGSYLLEQVARIPAQAELASELR
                     YRNPVVEQGSLWFVVSQSGETADSLFAMREIQRKGATVLGICNVVGATIPRESDGGVY
                     VHSGPEIAVASTKAFTSQLTVFFLFTLLMARMRDMSREEGKIFIRALTGVPDMVRDVL
                     SKRDRIQAIAKKYCRTKDFLYLGRGILYPIALEGALKLKEISYIHAEGYSAGEMKHGP
                     IALISPEVPSVFLVSDDYLHDKTISNMREIKARGGPVIAICSDEDKEAMSFADDFISV
                     KKADPRFYPFSMIIPLQLFAYFCALELGCNVDQPRNLAKSVTVE"
     gene            7953..8771
                     /locus_tag="PK105_01430"
     CDS             7953..8771
                     /locus_tag="PK105_01430"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOU60396.1"
                     /translation="MRILSYKSIDSTMDEARRILQEGLVKHITIVRADTQTAGRGRIE
                     GRAWIDMPKHCLLMTIILPQSYKDISAIPLRAGLGVVRALENSLKDPALETQPFPELM
                     DNYGQHEHKLAPYFLLKWPNDIVAHIPGDSKCFGKLCGILCEQTADTVLIGIGLNLLK
                     SRYSGLNTDLSPLSLEEALGFIPRAFANLDDAACSITEHVIQALHDPTWHSEYEHRLW
                     GRGALMHFIIGHPRLAQEVEGICVGTSEDGSLILSNNGKKRKFVSGEISLLRFV"
     gene            complement(8754..9692)
                     /locus_tag="PK105_01435"
     CDS             complement(8754..9692)
                     /locus_tag="PK105_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOU60397.1"
                     /translation="MKNIVSVVVVGAGAIGASIAVRLMEAGKQVVISANGERKARYLK
                     QGFVVNGRQYFLPVKDKYEADPADLVIIAVKNYSLEEAIEEMRPYVSVNTIILSLLNG
                     IDAVPRLRREFGEDSVPYAMILGIDALREGNRVQYLAKGRIFCGFEKEKVEKNAPTLA
                     VLEEFFGTSDIAFVVPEDIVKEIWFKFMINVGLNQWSALIRAPYRLFQDSPHGQELLS
                     KTMIEVISLSKRYGGNLEETDIDRAIAVLKTLAPQGKTSMLQDVEARRQTEVDAFAGA
                     MKRLAKAADLDVPINAILYDAIRAIEDSFEDSYKAE"
     gene            complement(9724..11031)
                     /gene="hflX"
                     /locus_tag="PK105_01440"
     CDS             complement(9724..11031)
                     /gene="hflX"
                     /locus_tag="PK105_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOU60398.1"
                     /translation="MTGVKRGEQETSRVFLIGVASSEIPRSEAQELLDELHRLTDTLG
                     NEVAGEMLVLLRESTPSLLIGTGKAQEIVDAADQAGADTIIFDHVLSPVQQRNWEKLS
                     SKNVYDRAELIIKIFASRAFTKEASLQVELAQLEYALPRLAHSYNDLMQQRGGRYGTK
                     GSGEQKLELDRREIERRIHEIKGELETVRKERAVQRKRRERSAVPRAAIVGYTNTGKS
                     SLLNELTLASVHAEDKLFATLDPTTRRIQLVRGQTLLLTDTVGFIRNLPPGLVEAFHS
                     TLEEVSLADVLIHVADASDKSIDKHIETTIKVLFDIGAGEIPHILVLNKIDLVDPVVV
                     QAFLSRYPGSIAVSAKTQEGLGELVKEIQEALTKQMETLMLRIPHADNHVVSLILREA
                     TVLDQYYDEEYTWIRCRIPRKLIRAVQKYAYKGALPLMDSIKP"
     gene            11151..12521
                     /locus_tag="PK105_01445"
     CDS             11151..12521
                     /locus_tag="PK105_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU60399.1"
                     /translation="MPLQKSDLSDAVINTYYAVRGPIVARAQQLEQAGKEIIYCNIGN
                     PQALKQKPLTYVRNTLAACEQPGLLNISDNFFMEDIKEKARHILEQSKHGLGAYSESK
                     GIRFVRNAISDFIKSRDSFDGVEQTADPEHIYLTDGASKGVQAALTLLIAEKNDGILI
                     PIPQYPLYSATITLYNGQQIGYYLDEETGWSLDERMLEDAIQEALAKGIRARAIVVIN
                     PGNPTGGVLTEDNIRMVIRFAKRHNITILADEVYQENIYKPGARFISFAHVMTSMREQ
                     EVSLFSFHSVSKGFFGECGHRGGYMEVRNLPCEVIDQITKLQSISLCANLPGQILTYL
                     MVSPPKPGDPSFQQFANERNAILTELAKRAKMLHEGLNSIPGYRCQPIDGAMYAFPSI
                     TLPPGKSDDDYCMALLEQTGVCVVAGSGFGQKPGTAHFRTTILPPTEQIERVVDALGK
                     FHRTWK"
     gene            complement(12536..13267)
                     /locus_tag="PK105_01450"
     CDS             complement(12536..13267)
                     /locus_tag="PK105_01450"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU60400.1"
                     /translation="MKESCAILYADQDLLAIDKPAGILAIPDRWDPNIPVAQQLLQSQ
                     FGTLLPVHRIDKDTTGVLLYARNEVAHRIISCDFSSRKIEKIYFAIVHGQPESQAWEV
                     ELPLRADGDRLHRTIIDASKGKEAKTRFEVIERFRGFALVLAIPETGRTHQIRVHLAA
                     SGLAIVADPLYGNGDPLLLSMIKPGWKGDAYKERPLIARTALHSAKVSFIHPKTKKEL
                     LIEAPFPRDFKATLAQLRKVRPSWV"
     gene            13531..15168
                     /gene="groL"
                     /locus_tag="PK105_01455"
     CDS             13531..15168
                     /gene="groL"
                     /locus_tag="PK105_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710555.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOU60401.1"
                     /translation="MAKQLLFSEDARRKLLVGVETISEAVKVTLGPKGRNVLLDKKFG
                     APTVTKDGVSVAKEIELEDPYENMGAQLLKEVATKTNDIAGDGTTTATVLAYSIVKEG
                     LKSVAAGIDPMALKRGIDQAVTFSVEEIRKISKEIKEKEEIAHVASVSANNDIDIGNQ
                     IADAMEKVGKDGVITVEESKTMETTIEYVEGMQFDRGYTSPYFVTNRDSMTTVFENPL
                     ILIHDKKISNMKDLLPILEKIAQTGKPLLIIAEEVEGEALATLIVNHLRGTLNVCAVK
                     APGFGDRRKAMLEDIAILTGGEVISEELGLKLENTSISQLGTAKTVKVDKDNTTIING
                     GGKQKDIQDRIAQIKMQIEETTSDYDREKLQERLAKLAGGVAVINVGAATEIEMKEKK
                     HRVEDALSATRAAIEEGIVPGGGIALIQAALALDKADTSKMADDERVGFKIVKRALEE
                     PIRQIAENAGIDGSIIADKAKQEKKGIGFDAAKLEWTDMTKAGIIDPAKVTRYALQNA
                     ASVAALLLTTECAITDLPEKNPPAMPSPNAGMGGMEY"
     gene            complement(15260..17209)
                     /locus_tag="PK105_01460"
     CDS             complement(15260..17209)
                     /locus_tag="PK105_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60402.1"
                     /translation="MNTNDKQENFDNSSDFPDTQMKRRQRTKPVPPPGRRGLMAGEKS
                     RDFKGTMKKLIAYLAPYRWPILFAIIFAIVSTILAILGPKVLGNATTTLFEGVVKKTT
                     RTGDIDFAKIKTILLTTLALYLSSAILQYFQGWVLSGIAVKITYSFRKDILAKINRMP
                     FRAFDSTNHGEILSRITNDVDVVNQTLTQTISQMIHSIMAIIGVFVMMLTISWQMTII
                     ALVTIPLSLIVVRVIVKRSQKYFRIQQEYLGHVNSHIEEIFSSHIVVKAYGGEQKAVQ
                     TFNNLNSTLYRSAWKSHFLSSIMMPLMNFVGNIGYVAVAVSGGWLAAKKMVTVGDIQA
                     FLQYVRQFTQPIVQIANISNVLQQTAAAAERIFDFLASEEEKNVSVESPQNGALVEIP
                     KSIKGKVEFRHVRFGYLPGQPIIKDFNAMVLPGQKVAIVGPTGAGKTTLVKLLMRFYD
                     PDSGEILIDDIPIGNLPRSVVRSWFGMVLQDTWLFSGTVRNNIAYGKRDATDAEIIEA
                     AKAAHVDHFVRALPEGYDFEINEETTNISQGQMQLLTIARAMLAQPPMLILDEATSNV
                     DTHTEVLIQRAMGVLMQGRTSFVIAHRLSTIRDADLILVMDHGDIVEQGTHQELLSKG
                     GFYAELYNSQFDQNGEDQLANASGL"
     gene            complement(17206..19404)
                     /locus_tag="PK105_01465"
     CDS             complement(17206..19404)
                     /locus_tag="PK105_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60403.1"
                     /translation="MRTILHFLKPYWLNIVLIILFLFIQANADLSLPDYLSRIVNVGI
                     QQSGIEADLPAVMRKSTFEALQKLLQMEARPEDFTALQEVYIEVLPDSPKAQSLSKKW
                     PIAKKESIMMLSSEDKAELDAARKVFLSEYPKLAAFLQFSSMAASKASPQMSSSDLSG
                     LSSMTPQELSAQIEALDPIIKSQIIVLGLQGEFTAMGANIGAMQRSYILRVGIQMLLI
                     TLITVGSAVSVGFLGSRLSAGTSRDLRNALFSKVEEFSLAEFDSFSTASLITRSTNDI
                     QQIQMLIMMSTRMLFYAPIIGIGGIIRALGKASGMWWIIAMAVGALLAIIGLVFALVV
                     PKFRIVQKLMDRLNLVVRENLSGMMVIRSFNMQDQEAKRFDKANRDLTSMMLYVTRAM
                     VVLMPLMTIIMNVVSISIIWVGSHEVAVGSIRIGDMMAFMQYSMQIFFAFLMMSAMFI
                     LFPRASVSAERVAEVLDKEVSIRDPEKPVHLSKPVKGEIEFHQVDFKYPGAQEQVLED
                     ISFIAKPGTMTAIIGTTGSGKSTLVSLIPRLYDVTGGQILLDGIDIRNLPLSELRAQI
                     GFVPQKSILFSGDIEENIRYGKENALENEIMEALEVSQVLSLVEESPEGLKRPISQGG
                     MNVSGGQRQRLAIARALVRKAPIYIFDDSFSAIDYKTDRAIRSALKRYAADSTIFLVT
                     QRVAPIRHAEQIIVLHEGQIVGIGMHEQLMKTCTVYRDIALSQLKQEELV"
     gene            complement(19665..21272)
                     /locus_tag="PK105_01470"
     CDS             complement(19665..21272)
                     /locus_tag="PK105_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOU60404.1"
                     /translation="MSDRYSIIPIPVAYESREGNFRFDKETWLIAKPGLEKETEVLAA
                     WLSRVPGIEKVKIVSANSNISESLFMPSGHERNKPEGSMILLSLEPELFFNTPEGYIL
                     DIAPSCIQIKAAESAGIIRGAASLWQLALGNGRKEEQGVSIQAGKIVDEPRFGWRGLM
                     LDCARNFFRVEFIEKLLDLAALHKLNVFHWHLTDDQAWRLEIPSKPELVQYGAFRRDL
                     RYQEEYKKGGYYSQSDVKRIIDYAQARHILVVPEIELPGHSIALLASHPEYSCKGRAD
                     PTVHFEPEDRYGIFEDIVCAGNDDVFVYFDDVLQEVCKMFPGPYVHMGGDEAPKTRWL
                     ECSDCRERMNTLGFVSEDSGYLPEKLQAWFMGKISNLLFKYGRRMIGWDEVIDGDIRR
                     DTIVMSWRGIEPGIHAAKRGYDVIMCPQTRACYLDHKHNDEPEEPGHLGVCTLSDSYL
                     FEPVSDKLSDEESDHILGGQANLWSELIYFGRQAEYMLFPRLCALSEVFWSPRGKRSF
                     EDFSQRLRVHEHRLDALNVCYYKGRFF"
     gene            complement(21283..22623)
                     /locus_tag="PK105_01475"
     CDS             complement(21283..22623)
                     /locus_tag="PK105_01475"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOU60405.1"
                     /translation="MNREDGFPQDFIWGCSTSCYQIEGAAKEDGKGISIWDTFAHTPD
                     KVLSNHTGDIACDSYHRWKEDIALLRELNAGAYRFSVSWTRIQPEGKGAPNKKGLDYY
                     SRLVDGLCEAGVEPWLELFHWDLPQALEEKGGWRNRDTAMRFEEYAGIMYDHIGDRVG
                     HWTSMNEPWCAAFLGHLTGIHAPGLKDRSATNRAVHHLLLAHGLALRAFRRSGIKGEY
                     GLVINPSKPRPATLRPEDAEASERASIERTSLWLDPIFGRSYPRVFMDIFGSDMPIEA
                     GDMDIISEPLDFIGVNYYDEEAVRAAPCSAQNPYGFEFVPTYQKKTDMGWEIEPSGLR
                     RILSYIANVWPCKKIYVTENGAAFPDKQDADGIVRDYDRIDYIKGHANACGDAIKQGV
                     PLKGYFLWSLIDNFEWSFGYNRAFGIVAVDRNTLERRPKLSFYYYRDLIAGFGL"
     gene            complement(22649..23500)
                     /locus_tag="PK105_01480"
     CDS             complement(22649..23500)
                     /locus_tag="PK105_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU60406.1"
                     /translation="MIKESKCKNILSIIGKAAVYLIMIIFTFMTLYPIFWLIMNSFKT
                     TREFQISQLALPKSPTLQNYKGAWEIGDFSLLFPNSILYTLGATLGIIFLSLLAGFAF
                     AKLKSKATKPIYNSFIMGILLTTQTLMIPLFLEVNLLGIYNTRFAVLLVYIGAGLPIG
                     IYLSTEYIRAIPSAVVESARIDGAGFFRIFLSIIMPMSVPVATTLAILNITNTWNEFA
                     LINILVSKTELKSLPLGIYKFSGSLSSDYGKQFAALTIGMIPMLVFYMIFRKQITKGV
                     AAGAIKG"
     gene            complement(23513..24637)
                     /locus_tag="PK105_01485"
     CDS             complement(23513..24637)
                     /locus_tag="PK105_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU60407.1"
                     /translation="MAFSSKSYIEEKRAYWAMVMPAFGIYLLVMAFPIVLSIVLSVSN
                     YSGGKMFGGEKWGFAGFDAYLRVFRDPWFWNALKNNIYIVLISVFGQLPLGFIFAYFI
                     YAKIVKAPEFWQGVLYVPNIISVIVVGLLWQTIFSPHGPIAEVINSIRVREFQSQLRG
                     IFDAAGGFSLSDDVVKRILDLVGPSGQAMFSDPLVDLRDFLSSFHGEGIKEVYNALSN
                     LFVQKWSPAFLTKPNIAMLPILFVILWMYTGMYLILFLANMQKIDVQVVESARIDGAN
                     EGQVMRYIILPALSGTIVNAAILAISGSLSSFALIFAMTGGGPSRVTEILSIYMYNNA
                     FLGRPNFPLANAISLIMVLISFGLIALTKAVEKRYGGKEE"
     gene            complement(24733..26037)
                     /locus_tag="PK105_01490"
     CDS             complement(24733..26037)
                     /locus_tag="PK105_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU60408.1"
                     /translation="MKKALLSLLTLFVVLGAVVAQEKVTLNVLNYQQADQAGYQEDVA
                     IWEKFQKDNPDIKLNIEVLFSEPYHQKLQVYAAAGTLPDVFYVWPTSRSAIIHEKKLA
                     KDLSKLLGPDFLKDFSGAATDPNNQLGKYLAMLPQAFTYTSVMFVNKKLLADNGFDLP
                     KTYDDLKRMVWKLKAKGINVIMLPNKDGWPMQSCLFSTVLGRMAGDDFVDAILAGKAK
                     FTDKPFVDSLKVIDNLYKDGIMAREDNQVGYGEGPGLFVSGKAAIYIDGDWRAGAYIT
                     DKASGKALIPPASQEKDFVLLPFPSIPGEVNPGVVSGIAGTGWAISAAIPSGSAREKA
                     AVKLFKYLYSEEVQSIRYSTGSYVPTRKNVKANVEPLVAKVPQFYADNPKVCYVFDGL
                     FDPAVYNVLNDGLIAIGLGTATPEKVAADVQKAFETWKASSK"
     gene            complement(26120..27271)
                     /locus_tag="PK105_01495"
     CDS             complement(26120..27271)
                     /locus_tag="PK105_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOU60409.1"
                     /translation="MPPRGDQWSRTAARIFRTIWRNPSISRIDLAHKLDLDKSTVTKQ
                     VAHLIENGLIVEQEEGEASARGGRKPICLSANKEYGHFIGIELQLGFYDAVIVDLSGN
                     ILATLRGDTHVTSDNFVQVVLQIIEETRKQRCDSSLLLGVGVGTAGLIDSKKGRIRYS
                     VPLGIDRTLDFAKAMEGKLDVPCFIENDANCCAWGELAFNRVEELKDFLFALVEYRKD
                     ETSLLKYGGIGVGFGIVLGGKVHTGAHGYTGEFRSAFCNGPGDLQFSLTRNELMLLRF
                     DRGLLNRAVDELARNMAMLINTMDFQQVFIGGNIEALDVDLPTVLRRRLKENWMYPSP
                     KDVKIRYSSFGDKAVAFGAAGMVFERLLSEHRLPGLASSFSSDNIGRIS"
     gene            27456..30047
                     /locus_tag="PK105_01500"
     CDS             27456..30047
                     /locus_tag="PK105_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOU60410.1"
                     /translation="MEVIDLAGNWNLVRSSDTTNRPIHIPGDIYSALMISGELPDPYQ
                     GENELMVQWPGKEDWIIERSFFISEAFLAASSIRLCADVLDTIAEVYINKVLVGTGKN
                     MFRPFEAEVRPALHPGENDIQVIIRSPVQAALRESQRLPYPIPASIYPVSSPHRNLIR
                     KAQCMSGWDWGPCLMTGGIYDGIRLVATDGLFIRSIQCHASALGDFGSRNDCGLDFSL
                     QISVEIEVPKPMKVVVSSYIDGKKATVEQECEQGNTVVSTNMVIQNPRLWWPHGYGEA
                     ALYDLVVDAAVQTANPPQPHVIHKRIGFRSLQVISQEDSIGRSFKFLINGKEIFAKGA
                     NWIPADALPSRWTRARIEHLLNSAIDANMNCLRVWGGGRYESDDFYELCDQKGILIWQ
                     DCMFSCALYPSSREFLSNVEAEITAQVKRLSDHPCIALWCGNNEALGAITWYEDSKKN
                     PARYIVDYDRLTEGVLGNVIRKLDPDRCFWPSSPSAGPDDFSDNWHSDERGDMHFWSV
                     WHEGKPFSAYLDVQPRFCSEFGFQALPCMSTIASFAHREEWNIGAPALEAHQKNPQGN
                     SIILESLLRYFRMPSGFHELLYLSQVQQAIAICTAVEFWRSTMPRCMGTLYWQLNDVW
                     PAISWSSLNYDGSWKLLHYEARRFYAPILLALFIKDGIIHVRLVSESPNNHKAKVYIK
                     LIDFYGRPIDIPSKEQGPIIEHVVSIEENQVYQVWEMPIVELPCKANQAFLEATLEVP
                     EIGLFSTSILFLTEPKRCALSDPKLETSVCYDENRVLKATIKATDAPAFFVMPYLEGI
                     DGQFDDSGFYMPKGSTKILSFSPINAADATVKQSQHKQSQHSQRLRAAEKTFMPALRV
                     FHLQNSF"
     gene            complement(30093..31607)
                     /gene="guaB"
                     /locus_tag="PK105_01505"
     CDS             complement(30093..31607)
                     /gene="guaB"
                     /locus_tag="PK105_01505"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOU60411.1"
                     /translation="MNLEKLLQEGAGLTFDDVLIVPRYSEVLPSEVDISAELVPGIRL
                     KAPVLSAAMDTVTDSRLAIAIARIGGIGIVHRNLSPEAQAAEVYKVKRSESGMISDPV
                     WLPETATLAEAEHLMETYRISGIPVVDPLTGRLVGIITNRDKRFCGPQDMPKPVSEFM
                     TSKNLITAPEGTSIEEAKAIFRRYKIEKLPLVDEEGRLKGLITLKDIVKKEEYPNAAV
                     DARGRLLVGAAVGVGSDLEERVSLLLSRNVDVLVIDTAHGHTKKVIEAIRRIRSLSGD
                     IHVIAGNVVTAEGTQALIQAGASAIKVGVGAGSICTTRIISGVGMPQLSAIYECAQAA
                     KAYGIPIIADGGIRYSGDIVKAIAAGAETVMLGNLLAGLEEAPGELVFFEGRQFKSYR
                     GMGSIGALQGYGRDRYGSGQSDASTDRQLNVRQSGKAVPEGVEGMVPYRGKLSDYMVQ
                     MLGGLRSGMGYAGARTISELRKARMIRITAAAYAESHPHSIVISKEAPNYQKRD"
     gene            complement(31654..33450)
                     /gene="purH"
                     /locus_tag="PK105_01510"
     CDS             complement(31654..33450)
                     /gene="purH"
                     /locus_tag="PK105_01510"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOU60412.1"
                     /translation="MPIALISVYDKTGLVPFAKRLAACGWRFLASGGTAATLREAKLE
                     PLNIAAYTGSPELLGGRVKTLHPAIHAGILARSSEDDLQELHAHGYEPIDLVVVNLYP
                     FEETVRRLKMGKKALAEKRGKIKPSGSLADTKVPNIDEGMVSDAITINANTAPIANNT
                     MDAEAAEIVPSASLDESEIIEQIDIGGVALLRAAAKNHARVGALCDPLDYDLVACEIE
                     KGGLSSSTLRALAAKAFARTRDYDAAIAAWFLESNAESLNSDEFALPAAGLTSFGAEL
                     GNEEPGGVFSLVGRVERTLRYGENPHQKAFFVLPKTRSIRSIIRGPLGGQVLEGKELS
                     YNNLLDLDAAWRAVLDFEGPAAVIVKHLSPCGAAEADSLQEAYRAALACDPVSAFGSI
                     VALNRPLDLETALMMKGLFLECIAAPDFDEAAREILKAKKNLRLIAADPLLFEKEARE
                     LRSAAGGILVQEQDRGDPMNTKWSVVSDRKPTAAEWEAMRFAWKLVRHVRSNAIVLAA
                     GRAAVGIGGGQTNRVDAVRQACERAADRARGSVLASDAYFPFADGIEAAAGAGVTAVV
                     QPGGSVRDAEVLAAADKLGITMVYTGVRHFRH"
     gene            complement(33422..34165)
                     /gene="purN"
                     /locus_tag="PK105_01515"
     CDS             complement(33422..34165)
                     /gene="purN"
                     /locus_tag="PK105_01515"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOU60413.1"
                     /translation="MMTKNDGNRVSDSIVRIVVMASGEGSNLQALIDAIESGWIENMR
                     IEALLCDKPGAHCIERAICHGIPSHILPAPQKAKRSSPERRCYDERLAAVANSYKPDF
                     IFLLGWMRLLGRTFLENFPFRVINLHPALPGAFPGTNAIERAFEAFNQGRIANTGVML
                     HFVPDEGIDSGPVLSVVHVPINKGDTLSILEERIHSVEHRELINLARRLANNPRPKEI
                     QSQEMQETRDQDKLGEKESGHAYRIDIGI"
     gene            complement(34162..>36283)
                     /gene="purD"
                     /locus_tag="PK105_01520"
     CDS             complement(34162..>36283)
                     /gene="purD"
                     /locus_tag="PK105_01520"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOU60414.1"
                     /translation="AAQIESSKAFAKAFMMRHGIPTAEGKSFANPEEAKAWAKDFGRP
                     LVVKASGLAAGKGVIVPENWKETEAAIERLAAEGEIVLEERLEGEELSLIALCDGKDY
                     VVLPTARDHKRLLDEDKGPNTGGMGAYSPACSMEDAERMACATIAPALSGLYEEGYPY
                     IGALYAGLMLTAEGAKVLEYNARLGDPETQVILPLFDSDVLISLSACAHGRLGDARPR
                     FKAEHAACVVLASLGYPDQPVKGKPITIGDLPEKAYCLHAGTRLENGILVSSGGRVVS
                     VLGIGSARAEALKAAYEAVSQIHFEGKQFRRDIGSKAERVEWAVKRALSAGTKESSVY
                     AQVEESNPSSAYVRAGVDIDAGNMAVELMKRAVRSTYNSRVIAGIGAFGGQYDALLVK
                     DCKAPVLVASTDGVGTKTSLALKLDRLSHGKHKRGEPKDKRNNGSEENKGIWLTGLGA
                     DIVNHSINDILVQGARPLFFMDYIAADKIDPAKVAKIVEGMSEACRQADCVLLGGETA
                     EMPGTYREDEMDIAGTIVGIVERDKLLPRSDIAIGDVLIGLASSGLHTNGYSLARSIT
                     SSMELERVYPELGESLASALLRPHRSYLPLLAAILNANPCPIKALAHITGGGLIENIP
                     RVLPPYLDAQIYAESWRWPALFLLLQRWGNVPDEEMRRVFNLGIGMVAIVEESQLNSF
                     LNMLPEQAFVIGRLVSGRGNVVLV"
BASE COUNT         9636 a         9096 c         7922 g         9629 t
ORIGIN      
        1 tccttcaaag aatatgaaag agttattgtc acttgctgct cctgcataca gatagagatt
       61 gtttcttttt gtgccagccg gaaccttgga gttgagataa tcgccccatg cttttccaac
      121 ctcaaagctg tcaaaggtta cgtagtagtc gacgctggtt gtatcgcgaa ttaatcggtc
      181 ataggaaata accttgacgc ctgcgctatg ggcgagatcg gctgcagacg cagcagcggt
      241 tccatcatgc gggcaaatga ttagaacttt tatgccttcg cttatcaggg cttcgacgtt
      301 agctttttcc tttgcggaat cgccttggct gaagagaacc tttgcactga atccagcagt
      361 ctttagagca tccaaaaatt gagtctgatc ttgaatccag cggggctcat ccttggtggg
      421 aagcacgata cctacttgtg tttcggcaaa tacaaagccc gcaactactg caaacaacat
      481 tgcaatcaat aatgttcgtt tcatgaggac tcctccttga aaaccgtaga ctctagtcgc
      541 aatacgacta gtctggcgct tcaattaaat tcggtacgcg ccaactatca ttttccgcta
      601 tattttttaa tcgcgcatta ggaattatgt gataaaaacc ggtatttgcg catatattgc
      661 cagagcaacg cagtattagg acttttagga caagacagct tcaaaaatgt ccagaattag
      721 aatctcgata gtctatatat ttattcagtg ttttacattg cgatatacat catccctact
      781 atgaaagcca tcctctatca ctgttgattc caatgtcttt gcggttacga gaactggctc
      841 gagcgcaatg taaggaacct tataggtacc atcttctatt ttgttctcat atcctatttt
      901 ttcgcctttg gccatcatta ccgcaaagcc agccgccttg agcgccaagc tgtcgatagg
      961 cttatagacg gttgcaaact gacttccttc ggcaatgcgt tgacaggctg aaagctcggc
     1021 atcttgtcct gtcaccttgg tctttccaac cattctattc tccgagagca cggatatcac
     1081 tgtctctgca agcatatcat taccacatag aactgcatcg atcttttgac ccggttttac
     1141 aagagcttca aattgtgtcc tcacctcatc gctcatccaa tcgcttggcc aaatctcttc
     1201 cataagttgg atcttgccag attcaatgaa tggatcgagc accgaatgaa aacccttgtt
     1261 tatcatgaaa gcattgttat ctgtagttgc tccatttatg atgacataat tcccctgcgg
     1321 aacaacttcc acaacagctt gagcctggag gcttcctact ttttcattat cgaaagaaat
     1381 gtagagatca acattagctc tatggactaa tcgatcgtag ctcaatacct gcacaccttt
     1441 tctttttgcc tcgcggcata cctccgaaag cctttccgca tcatttggaa taattacaag
     1501 cacatctatg ccttgactca acagctctcg gacttgcgct atttgggtct ctgcgttctg
     1561 attcgccact cgaagcacca cctctgcccc tagatcgtgt gctgctttgg tgaagctatc
     1621 cacatctcgt ctccatcgct cgaccacaag agaatccaaa gaaaatccaa tcaaaggcga
     1681 cttggtctca gatgtctttt ccgatttgct gcatgataat aaaccaagag cggtagttgc
     1741 taagatcatg gctatcaaga aaaaaatagg accactggcc ttacgcatcg cttcaggcat
     1801 cgctatcttc tccctttgta acccgtgcag catattcgcg cggcgtaagt ccggtaagtc
     1861 ggcgaaacag acgagcaaaa taattagcat cggaataacc gcagcgctgg ctaacttctc
     1921 gtatggagat atttggcatt ttaagcaatt ctttggcttt ctgcattctg taccctatca
     1981 gagtcctcgc gaagcctctc ttcagctcgt ctgacataag acgcgtaaga tgtccggcgg
     2041 aaatgcctat tgccgctgat gcactctcta atgttatcgg ttcctggtaa tggttctcta
     2101 tatactgcaa agctttgaca acaaaaggcg aatgagtccc cacctccgcg gcgtgttgtt
     2161 gcagactctg gaatctgcct cttaccgctg atgcaaataa atgccaaaag cgctcctccc
     2221 acagtttttc gatatcccca aaatccatga aatctctata gacattatcg gagagcactc
     2281 ctccgcccgc aagcttagaa gaccagaatg aaagtgctgc tattattcgg aaatatagat
     2341 cgcgcggttt gtcttgcgtc tcttccacgg tcaagatgag cttttcaaga agctgcccag
     2401 ccatggcata ttgaccttcc atgatcgcat catagaactg tgtatcgaat acggaagctg
     2461 gttcttctcg attttcggca atatcttctc cgaggcgagg cctgtagcga tttatcgatt
     2521 ctaagccaag cccaaagtta cgtacggcat tatcccaaga gcgcccaatg ttttcaagca
     2581 attctggctg accacaagca aatcgcaatt cgcccgttgc gagctgtgat gaaaaggatg
     2641 aacctagcaa ttcaagaaaa gccgtgatct cgatctcagc gacagtacct tgatcaatcc
     2701 cagatttcaa tggcaagaac catgcacaat agcggttatc ttcgagcggt ccagctagag
     2761 ccagggtttt gtattgcaag gtagagcaaa aatgctcata gagcgaattt gccaagccat
     2821 ctgccggaga aaaacttgca atacccataa caccggtttc tcctgaaatt tgtagcactt
     2881 ccttgtaaag attgagctct tctttcaagg cctttgtgcg tttggggtcc cttggttctt
     2941 tgtctttagc ctcgcgctgc tgccgcaaat atacaaaaaa agcagtttta ataaagggca
     3001 atagtgtttg ctggcgctct ttgaattcaa gctctttttc ctctagtata tgaagacgat
     3061 cgagataaga agatgccgcc tgcaaggcaa tctcgagcct ttccttggaa acgggtttta
     3121 gaagataatc gcagactccc atagaaaggg cttcccgcgc aatatcgaat cgctcatatg
     3181 cagtcacgag aataaatgcc ctggctccac cttgctggga gagttctcgt attgcgtcca
     3241 gcccattgat tccaggcatg tgaacatcca taagcactat gtctggtgca agagttttgc
     3301 ttttttcgat tacttcgcgg ccagactgag cagtcccgac caccagatag ccatcctgga
     3361 aatagcgttt gaaaagcagc tgaagaccat ttacgatcgg aatttcgtcg tcggctatga
     3421 gcactctctt catgatattt ttcctgcagg caagaggatc gtaacacaag taccattgcc
     3481 aagctcgctc tcgatctcga ccctaccctg acctcctgtt gcgagagcga ctcgccttac
     3541 gacattgtgt aatccgatac cattttgagg ccttgtatct tctgggcttg ctgctgcaag
     3601 agcaagggcg atgcattcat tactcattcc acagcctgta tcactaaccg agaaatggat
     3661 tgtatctcct tccagcttag ctgatatcaa tactcgtcct ccttcttcga tatcctttaa
     3721 tccatgtaca attgcattct cgacaagagg ttgaagcacg agtgcgggcg tctcacagcc
     3781 aagggcttca tcctgtatgt caaggcagaa ttcaaagcgc tcaccaaatc tcagcctcaa
     3841 aagccatata tattgtcgaa cgcattctat ctcctccgag acaagaacga atctagtaga
     3901 aggcttcaat gagtaacgta tgaatgctgc aagcttttcg aggaattcag cggttttctc
     3961 attaccttca gatagagcga tctgccaacc ggctgccaga gtattatata gaaagtgtgg
     4021 gttgatctgt gtttgaagag ccaggagctc cgcatccttt agcctgtgct gataagttag
     4081 cactcgcatg cgctcttcca taagcgaacg ctcaagctcc gcctttcttg tcaattctat
     4141 aaaagccttt tggacactat cctgcatgtt gacaaatgca tcatgaagcg ttcttatctc
     4201 atcctttcgc tcatatggcg gcagaggatg attatactct cccctgccta ctgcacctgc
     4261 agccactgaa agccgcgcca atggttcggt tattgtataa gaatatcttg caattagtgc
     4321 gatcgagagc aagaaagccg caagcaaaag caccccattg gacataattg caaggcttat
     4381 atttgctcga tatgaagaaa aaccagtcaa tgattggccc aaatagatcg tatccattct
     4441 tgtactcaaa aactctatgt aagaagctcc acgccttgct tcctcgtagt agctaacata
     4501 ctctgtgaca tcgcggccgc gcttagcctg gacagccctt tcagcgctcg caagatagct
     4561 ctctacaaga taagccagat ccttctccag caaaaagaca tcgtcattct tattttcttt
     4621 attgagctta tgagccatat tggaaaggag tgtcgaattg cgtataaatt ctctaagcga
     4681 ttctgaattc ttagcactga gataacccat cagattttct tgtgttgccc tcaaaaccgt
     4741 tctgagttct tgcatcgata cagaattgtg aaatagaagc tctacacttc gagccaaatt
     4801 aaaagaagta gatgctgtgt aggatgttgc tatgactaaa acgagtagta ttactgcgga
     4861 attagcgatt attcgatccc gaagaccagt tctgctgcgc ctggctctca gaggtcgaga
     4921 gagccatgta aagtcgatca taaaccccct cctagtgcgg ggatcactcg gtatcctacc
     4981 gagattcttt caggagcccg aataccaatt cgctggccaa tcgctagagc cacgccagct
     5041 ttacccatag ccactgcatc tctaacgata cttgcctgga ccacgccttt ttcgattagc
     5101 ctattgacct ctgagttttc atcggctcct atgatgacaa tttgtcctac catgccgcgg
     5161 tctattacaa cctgagcggc accaattgtg gccttggcgt ctatgcagag gatcgcattg
     5221 atgtctgggt gttcagctaa catgcgcgcg caagcatctt ctccgccgag aatgctctct
     5281 tcttcgcgct ctgcagcgac aattgcggct ccttgtttgc ttttgagagt ttcttctact
     5341 cctctataga atggttcctc atagaaagca tagcccaaag atgtgtctga agagagaatt
     5401 atgccgatac gagccgcagc gcccagcata cccaaagcaa gcgcgcttgc ctccctcccc
     5461 tgactagcag aatcgccagt taccccgatg tttatccatt cctgagaaac atccattgca
     5521 agagggacaa acggtatttg tgcactttcg gctgcttcca taagcctctc aacatcgaga
     5581 ccggcagtct taaaaagaat tataccatca gttttgctgc gcaatgctat ctcgaaccag
     5641 cgcaacactt ctccggagat accaccacta tcggctgcgc cagtaggcga aaaataaaat
     5701 atctgcagtg ccgcatcctg ttcttctgct tcttcggaca agccctcttt caggtgcata
     5761 aaaaatgggt ccacagtatc cggaagaata gcaataatgt gaaaacgagc actatgaatg
     5821 gtcgatggat tgcgcagaat ggtagatgct agagcgttgc ggcttctgct gacgagagca
     5881 acgttgaaag caagtgcccc tacaagagca atagcgaata cgacaattgg caagacacgc
     5941 catgcaccat gcttcattct actgtcacgc tcttggcaag atttcggggt tggtcaacat
     6001 tgcagccgag ttccagcgca cagaaatatg caaacagttg aagtggaata atcatgctga
     6061 acggatagaa ccgcggatct gctttcttga cagaaataaa gtcgtccgca aaagacatgg
     6121 cttctttgtc ttcgtcggaa caaatagcaa tgactggccc ccctcttgcc tttatctctc
     6181 gcatgttcga tatagtctta tcgtgcagat aatcgtcgga aacaaggaag acactaggca
     6241 cttccgggct gataagcgca atcgggccat gtttcatctc gcccgcacta tagccttcgg
     6301 catggatata tgaaatctct ttaagcttaa gagcgccctc aagagctata gggtatagaa
     6361 tgccccgccc aagatagagg aaatctttgg tgcgacagta tttcttggct attgcctgta
     6421 tgcgatcgcg cttagataac acatcgcgca ccatatccgg aacacctgta agagctcgga
     6481 taaatatctt gccttcttct cgcgacatgt ctcgcatacg ggccattagg agggtaaaga
     6541 gaaaaaagac agtaagctga ctcgtaaagg ctttagtgct tgcaaccgct atttctgggc
     6601 cagagtgcac atagacgccg ccatccgact cgcgtggaat cgtagctcct acgacattgc
     6661 agattccaag gactgttgct ccctttcgct ggatctcgcg catggcaaag agagaatcag
     6721 ccgtctctcc agattggctc actacaaacc aaagcgatcc ctgttctaca actggatttc
     6781 tatatcgtag ctcacttgca agctcagcct gcgcaggaat tcgtgctact tgctccaaaa
     6841 gataagaacc agcaataccc gcatgccagc tcgtgcccgc agcgattatg cgcaccctat
     6901 ggacttcggc aagctggcga cgtgtaaggt tgaggcctcc aagcttggct gttgcattct
     6961 cttcatcgat gcgtccgctc atggcgcgcg cgatcgaatc aggctgttca aagatttcct
     7021 tttccatgta atgcatgaag ccactttttt ccattgcacc aagctcccag gtgatctcat
     7081 ccactttttt agcaatggag tttgaatggc ggtccgtgat ggaataattc tcctgggtta
     7141 tatcgacgac ctcaccatcg ttgagataaa tgacacgatt cgtataagca accatagcgg
     7201 taaaatcgct cgcaagaaac atctcgtctt ttcccactcc aactatgaga ggacttccat
     7261 tgcgagcacc cacaatgcga cctggttcat ccgcatggat acaggctatt ccataagtcc
     7321 cttctagatg ttgaagtgct gctagcactg ctgtaaggag gtctccttca taataagaag
     7381 cgataagatg aggaatgacc tcggtatcag tttcgctctt aaagacataa cctttttttt
     7441 caagcactgt gcgaagggtc ttatggtttt cgatgatgcc attgtgcacc accgcaattt
     7501 tgccggatat atccgtatga ggatgggcat tggcctctgt tacaccacca tgggtcgccc
     7561 atcgggtgtg gcctataccc cattggccat ccagatcctc tggaattttc tttgcgagtt
     7621 ccccgatttt tcctactgct ttgatgatct ggaggttcgg tttcggatca gttcttccga
     7681 ctgcgattcc agccgaatca taccctcgat attcaagtct tctgagtcct tcaaggagga
     7741 cttttgatgc ttttcttggg cctgaatagc cgattattcc acacatgaaa atcagtgtat
     7801 cgaacctttg attttttgca atagagaaat caaggatctg aaacagtata ttttcaatct
     7861 caccatggct cttgtcctta gatgtcgatt tcgatagcct tgaacatatg gacaataatt
     7921 cgcgctcatc ctcatcgatc gagccatctt ctatgagaat tttatcttat aaaagcattg
     7981 actcgactat ggatgaagca cgcaggattc ttcaagaagg tctggtaaag cacattacga
     8041 tagtccgggc tgacactcag actgcaggcc gcggtcgaat cgaaggccga gcatggatcg
     8101 acatgccaaa gcattgtctt cttatgacta taattcttcc gcaaagttat aaggatatta
     8161 gcgcaatacc gctgcgagca gggcttggcg tagtgcgagc attggaaaac agcttgaaag
     8221 atccagcttt agagactcaa ccatttccag agctcatgga caattatggt caacatgaac
     8281 acaaactagc gccatatttc ctcttgaaat ggccaaatga catagttgcc cacattcccg
     8341 gggattctaa gtgctttgga aagctctgtg ggatactctg cgaacagact gcagacactg
     8401 tcttgatcgg tattgggctc aatcttctaa aatcgcgcta tagtggactg aacaccgatc
     8461 tctcgcccct gagcctagaa gaagccctgg gatttatccc gagggccttt gccaatctcg
     8521 acgatgccgc ttgctcgata accgagcatg tcattcaagc cctgcatgac cccacttggc
     8581 attctgaata cgagcacaga ctctgggggc gaggcgctct tatgcacttt attattggac
     8641 acccacggct tgcgcaggaa gtggaaggca tatgtgtagg taccagtgaa gatggaagcc
     8701 tcattctgag caataatggc aagaaaagga aattcgtctc tggagaaata tctctactcc
     8761 gctttgtatg aatcctcgaa tgaatcctct atagcccgaa tggcatcgta taagattgca
     8821 tttatgggta catcgagatc agcggccttg gcaaggcgct tcattgcacc agcaaaagca
     8881 tcgacctcgg tctgccttct tgcttccaca tcttgaagca tcgatgtctt gccttgaggg
     8941 gcaagtgtct ttaagacggc aatggcacga tcgatatctg tctcttcgag attaccgccg
     9001 tagcgcttcg aaagtgaaat aacttctatc attgtcttac taagaagctc ctggccatgg
     9061 ggagagtctt ggaaaagacg atatggagct cttatcaaag cagaccattg gttaaggcca
     9121 acatttatca tgaatttgaa ccatatctct tttacaatat cctctggcac cacgaatgcg
     9181 atatcgctcg taccgaaaaa ctcttccagg acggctagcg taggagcatt cttttccact
     9241 ttctcttttt caaaaccaca aaagatccta cccttggcca gatactgaac cctattacct
     9301 tcacgaagag catcgatgcc aagaatcata gcatatggca cactgtcctc accgaactct
     9361 ctgcggagtc tgggcactgc atctatacca tttaggagcg atagaataat ggtatttacg
     9421 ctgacatacg gcctcatttc ctctatggcc tcttcgagac tgtaattctt gactgcaata
     9481 ataacaaggt ctgctggatc tgcctcatac ttgtctttta ctggcaaaaa atactgccgc
     9541 ccatttacga caaatccctg ctttaggtaa cgagcttttc tctctccatt agcggaaatt
     9601 accacctgtt tccccgcctc cataaggcgc acggctatag aagcgccaat cgcgccagct
     9661 ccaacaacca cgacagaaac gatgttcttc attttgcaaa tatcctttct aaagagtctg
     9721 taattatggc ttaattgaat ccatcaaagg caaggcccct ttatatgcat atttctggac
     9781 tgctctaata agtttacgcg gaatgcgaca tctaatccag gtatactctt catcataata
     9841 ctgatcgagt acagtggctt cgcgtaagat gaggctgaca acatggttgt cggcgtgtgg
     9901 aatgcgaagc atcaatgtct ccatctgctt tgtcaaggct tcctggattt cctttaccaa
     9961 ctctccaagt ccctcttgtg tttttgccga aacggcaatg cttccagggt agcgcgaaag
    10021 aaaagcctgt accactaccg gatctacaag atcgatcttg ttcaacacga gtatatgcgg
    10081 tatttcgccg gctccaatat cgaagagtac ctttatcgtg gtttcgatat gtttatcgat
    10141 gcttttgtcc gaggcatcgg ctacgtgaat gagaacatct gcaaggctga cttcttccag
    10201 ggtgctgtgg aatgcttcca caaggccggg cggcaaattg cggataaagc cgacagtgtc
    10261 cgtcaggagc aaggtctgtc ctctaacaag ctgtattctt cttgtggttg gatcgagcgt
    10321 agcaaatagt ttgtcttctg catgcacgct tgcaagagta agctcattta ggaggctcga
    10381 ttttcccgta ttggtgtaac ctacaatggc agcccttggc acagcgctcc tctccctgcg
    10441 cttgcgctgg acggctcttt ctttgcgaac ggtctccaat tcgcctttta tttcatgaat
    10501 tcttcgttct atctctcttc tgtcgagctc caatttctgc tcgccagaac cttttgtgcc
    10561 atagcgccct ccgcgctgct gcatcaggtc attgtaggaa tgggccaatc gcggcaatgc
    10621 atattcgagc tgagcaagct cgacctgtag gctagcttcc ttggtaaatg ccctcgatgc
    10681 gaatattttg attataagtt ccgctcgatc atagacattc ttagaagata gcttttccca
    10741 gtttcgctgc tgcaccgggc tcaatacatg atcaaagata atggtatccg cacctgcttg
    10801 gtcggcagca tctactatct cttgcgcctt tccggttcct attaacagag aaggtgtgga
    10861 ttcgcggagg agtaccaaca tctcacctgc cacctcatta ccaagtgtgt cggtaagccg
    10921 atgaagttcg tcgagcagct cctgtgcctc tgatctaggt atttcactcg atgcgacacc
    10981 gatgagaaat acgcgcgagg tttcttgctc gccccttttg actcctgtca tgcttttatt
    11041 catcatttta tattacacaa ttcatgcaga tttgacatca gtctcgacgc cccagcgttt
    11101 tgggtggtag aatatgataa tatccaagcg atatcaaagg aggagatctt atgcctcttc
    11161 aaaaatccga tctcagcgat gctgtaatta atacttacta tgcggtgcga ggcccaattg
    11221 tggctcgtgc tcaacagcta gagcaagctg gcaaggaaat catctattgt aatattggaa
    11281 atccacaagc cctgaagcaa aagccgctca cctatgttcg aaacacgctt gctgcttgtg
    11341 agcagcctgg acttctgaat atttcagata atttttttat ggaagatatc aaggaaaaag
    11401 cacgccatat cctcgagcaa tccaaacatg gccttggagc gtactccgag tctaaaggaa
    11461 ttagatttgt gcgcaatgct atttctgact ttataaaatc gcgcgatagc tttgatggag
    11521 tggaacagac tgcagacccc gagcatatct atcttaccga tggcgcatcc aaaggcgtcc
    11581 aggcggcgct cactcttctt attgcagaga aaaatgatgg cattcttata cctataccgc
    11641 agtatcctct ctattctgca acaatcactc tctataatgg acagcagata ggctactatc
    11701 tcgatgaaga aaccggttgg agcctggatg agcggatgct agaagatgca attcaagaag
    11761 ctctagctaa aggcatccgt gctcgcgcaa ttgtcgtaat aaatcctggc aatcctaccg
    11821 gaggagtgct aactgaagat aatattcgca tggttatacg ttttgcaaag aggcacaata
    11881 tcacgattct cgctgatgaa gtatatcaag aaaacatcta taagccagga gcacgtttta
    11941 tttcctttgc acatgtcatg acctctatgc gggagcaaga agtgagcctt tttagcttcc
    12001 attcggtatc aaaagggttc tttggtgagt gcggccatcg tggtgggtac atggaggtgc
    12061 ggaatttacc ttgcgaggtt attgatcaaa taactaagct tcaatcgatt tcgctctgcg
    12121 ccaatttacc ggggcagatc cttacctacc ttatggtaag tcccccaaaa cctggagatc
    12181 cgagcttcca gcaatttgcc aatgagcgaa atgcaatctt aaccgaattg gcaaagcgag
    12241 caaaaatgct ccatgaaggg ctcaatagca ttcctggcta ccgctgccaa ccgatagatg
    12301 gagccatgta tgccttccct tcgatcacct taccacctgg gaaaagcgat gatgactatt
    12361 gcatggcgct tcttgagcag actggagtct gtgtcgtagc tggcagcgga tttgggcaga
    12421 agccaggtac cgctcatttc cgaaccacta ttcttccacc aacagaacag attgagagag
    12481 tcgttgatgc tcttgggaaa ttccatagaa cgtggaaatg acttcttgct aagtgtcata
    12541 cccagcttgg gcggactttt cgtagctggg ctagagttgc cttgaaatcg cgcgggaatg
    12601 gtgcctctat taacagttct tttttagttt ttggatgaat aaagctcact tttgcggaat
    12661 gcaaagccgt tcgggcaata agaggtcttt ccttgtatgc atctcctttc cagccaggct
    12721 ttatcataga aagcaaaaga ggatctccat tgccatataa agggtccgca acaatggcaa
    12781 gcccagaagc cgcaagatgc acgcgtatct gatgtgttct gccggtttca ggaatagcaa
    12841 gcacaagagc aaagccccga aatctctcta ttacttcgaa acgagtctta gcttctttcc
    12901 cttttgaagc atcgataata gttcgatgaa gtctgtcgcc atcggctcga agcggaagtt
    12961 ctacttccca ggcctgagat tccggttgcc catgtactat cgcaaagtag atcttttcta
    13021 tcttacgact cgaaaaatcg cagctgatta tgcgatgtgc tacttcattt ctagcataga
    13081 gcaagacacc cgtcgtgtct ttatcgattc gatgaacagg caaaagtgtg ccaaattgac
    13141 tctgaagaag ttgctgagcg acaggaatat tcgggtccca gcgatcaggt atcgcgagta
    13201 ttcctgcggg cttatcgata gcaagaaggt cttgatcggc ataaaggatt gcgcaagatt
    13261 ctttcacgac tgctaatgta ccatagatcc gctagagtgt ctcgaacctc ttatatggag
    13321 ataagcaaag agaggttcag ttcttttgtt aagagaatat tccaaaggtc cagaatagtg
    13381 gtatcctctt gccgttttct atcatttttt atatcttatg acaggcctta tcaaagccga
    13441 tccaaagaaa atatccggct tcacaacatc gtctgattga tgtatgtgtg tacggccggt
    13501 aaaaacactg tcatttcagg agaacaaggt atggcaaaac aattgttgtt cagcgaagat
    13561 gcgcgccgaa agcttcttgt aggtgtagag acaatctctg aagcagtcaa agtgactctt
    13621 ggccccaaag gcagaaatgt acttctggac aagaaatttg gcgctcctac agtgaccaag
    13681 gatggcgtat ctgtagccaa ggaaatcgag ctcgaggacc cgtatgagaa catgggtgct
    13741 cagcttctca aggaagttgc aaccaagaca aatgacattg ctggcgatgg tacaacaacc
    13801 gctacagtac tcgcttattc cattgtcaag gaaggcctca aatccgtggc ggccggaatc
    13861 gatccaatgg cattgaaacg cggaatcgat caggcggtaa ctttctctgt cgaggagata
    13921 cggaagattt caaaagaaat caaggaaaaa gaggaaattg cccacgttgc ctcagtctcc
    13981 gccaacaatg acatcgacat tggaaaccag atagctgatg ccatggaaaa agtcggcaaa
    14041 gatggggtaa ttacagtcga agaatctaag acaatggaga ccaccatcga gtatgtcgaa
    14101 gggatgcaat tcgaccgcgg ctatacatcg ccatatttcg taaccaaccg cgattctatg
    14161 accacagtct ttgagaaccc cctcatcctc atccatgaca agaaaatatc caacatgaaa
    14221 gatctactcc cgatactcga gaagatcgct caaaccggca agccacttct catcattgcc
    14281 gaagaagtgg aaggcgaagc ccttgctact ctcattgtga accatctacg tggaacactc
    14341 aatgtatgtg cggtcaaagc ccctggcttt ggtgatcgcc gcaaggcaat gctcgaggat
    14401 atcgccatac tcaccggcgg tgaggttatc tccgaagagc ttggcctaaa gctcgagaat
    14461 acaagcatta gccagcttgg cacagccaag accgtcaagg tcgacaaaga taataccaca
    14521 attatcaatg gtggcggcaa acagaaggat attcaagatc gaattgctca gatcaaaatg
    14581 cagatcgaag agacgactag cgattatgac cgcgaaaaac tccaggaacg ccttgcgaaa
    14641 ctcgctggtg gcgttgccgt aatcaatgtt ggggctgcaa ctgagatcga aatgaaggaa
    14701 aagaagcaca gagtcgaaga tgctctctcc gcaacccgcg ccgctatcga agaaggcatc
    14761 gtgccaggcg gcggtatagc ccttatccaa gcagctcttg ctctcgacaa ggctgatacc
    14821 tcaaagatgg ccgatgatga gcgtgttggt ttcaagatcg tcaagcgagc actggaggag
    14881 ccaatccgcc agattgccga aaacgctggt atcgatggtt ccataatcgc agataaagca
    14941 aaacaggaaa agaaaggcat aggattcgat gcggcaaagc tggagtggac ggatatgaca
    15001 aaggcaggaa taatagaccc agccaaagtc acgcgctatg ctcttcagaa tgcggcttcg
    15061 gtagcggcgc tcctcttaac aacagaatgc gccatcaccg acctgcccga aaagaatcca
    15121 cctgcaatgc catctccaaa tgccggaatg ggtggaatgg aatattgaga gaatacccag
    15181 acagtaatac attggaataa aagaaaggcg cccttttcaa gggcgccttt ttaacttata
    15241 tccaattaaa tcataaatac taaaggcctg aagcgttagc aagttggtct tcgccattct
    15301 gatcgaactg gctgttgtag agctcggcgt agaagccgcc tttactgagc agttcctgat
    15361 gtgtcccttg ctccactata tctccgtgat ccataacaag aattagatcc gcatctctga
    15421 tcgtagagag ccggtgggca atcacaaagc tagttctacc ctgcatcagc actcccatgg
    15481 ctctctgaat caacacctcg gtgtgtgtgt ctacattgct ggtcgcctcg tctagaataa
    15541 gcataggagg ctgagctaac atagcacgtg caatagtcaa taactgcatt tgcccttgcg
    15601 atatattggt tgtttcctca tttatctcga aatcataacc ttcgggcaag gctcgcacga
    15661 aatgatcgac atgggcagcc ttggcagctt cgatgatttc ggcatcggtc gcatcgcgct
    15721 taccataagc gatattattt ctaaccgtcc ctgaaaatag ccaggtatcc tgcagcacca
    15781 taccaaacca ggaacgcaca acggatcgtg gcaaattgcc gattggtata tcgtctataa
    15841 gtatttctcc actatccgga tcatagaatc gcatgagaag ttttaccaat gtcgtcttgc
    15901 cagcccctgt cggacccacg atagctactt tttgacctgg caataccatt gcgttgaaat
    15961 ctttgatgat aggttgccct ggcagatagc caaagcgaac gtggcggaat tctaccttac
    16021 ctttaatact ttttggtatt tcgacaagag ctccattttg tggagactct accgatacat
    16081 tcttttcttc ttccgaagca agaaaatcaa agatacgctc cgctgccgct gcagtttgct
    16141 gcaaaacatt cgagatattc gcgatctgga cgattggttg tgtaaactgt cgcacatact
    16201 gcagaaaagc ttgaatgtcc ccaactgtca ccatcttttt tgctgcaagc cagcctccgc
    16261 tcaccgcaac cgcgacataa cctatatttc ccacgaaatt cataagcggc atcatgatgc
    16321 tcgatagaaa gtgacttttc caggctgacc gatataaggt ggaatttaga ttattgaaag
    16381 tctgtacagc cttttgttct cctccatagg ctttgacaac tatatgagaa ctaaaaatct
    16441 cttcgatatg actattcaca tgcccgaggt attcttgctg gattctaaag tatttttggg
    16501 agcgctttac aatgactcga accacgatca gcgaaagagg aatcgtcacc aatgctataa
    16561 tagtcatttg ccaactaatg gtgagcatca ttacgaaaac accaataatg gccatgatcg
    16621 aatggatcat ctgggaaata gtctgagtca gcgtttgatt caccacatct acatcgttcg
    16681 tgattcgcga aaggatctct ccgtgatttg tactgtcaaa agccctaaaa ggcattctgt
    16741 ttatcttagc taagatatcc ttgcgaaatg aataggtaat cttgaccgca attccagata
    16801 aaacccaacc ttgaaagtat tgaagaatag cagacgatag atatagggct aaagtcgtca
    16861 agagaatggt cttgattttt gcaaagtcga tatccccagt ccttgttgtc ttcttgacaa
    16921 ctccttcaaa tagcgtcgtt gtggcatttc caagaacctt tggcccgagt attgcaagaa
    16981 tcgtagatac aatcgcaaaa ataatcgcaa agagaattgg ccagcgataa ggagccagat
    17041 aagcaataag cttcttcatt gtgcccttga aatctcttga tttttcgcca gccatcaacc
    17101 ctctacgacc aggtggtgga actggctttg ttcgctgtct tctcttcatt tgtgtatcag
    17161 gaaaatcaga tgaattatca aaattttctt gcttatcatt ggtattcata cgagttcctc
    17221 ttgtttgagc tgagaaagcg ctatgtctct atatacagtg catgtcttca taagctgttc
    17281 atgcatacca attcctacta tctggccttc atgaagcaca attatctgct cagcatgacg
    17341 aataggcgca acgcgctggg ttacaagaaa aatcgtgctg tctgccgcat atctcttcag
    17401 cgctgaccga atagcacgat cagtcttgta atcgattgcc gaaaaactat catcgaagat
    17461 gtaaattggc gcttttctga ccaacgcacg ggctattgcg agcctctggc gctgtcctcc
    17521 tgaaacattc ataccacctt gagagattgg ccgctttagc ccttccgggc tttcttccac
    17581 aagactcagg acctgagaaa cctcgagagc ctccatgatt tcgttttcca acgcgttttc
    17641 ttttccatat cgaatattct cttcaatgtc gccagaaaaa agtatactct tctgaggaac
    17701 aaaacctatc tgagctcgta gctctgaaag gggaagattt cttatgtcta tcccatccaa
    17761 aagtatttgt cctccagtta cgtcatagag gcgaggaatt aggctgacaa gagtggactt
    17821 gccggaccct gttgtaccaa taatagccgt cattgttcct ggctttgcaa taaagctgat
    17881 atcttccagc acctgctctt gcgcacctgg atatttgaaa tcgacttgat ggaattcgat
    17941 ctcccctttg actggtttgg atagatgaac tggtttttcc ggatcacgga tgctgacttc
    18001 cttatctaga acctctgcaa cgcgttccgc agagacactc gcccttggga aaaggataaa
    18061 catggctgac atcatgagga atgcaaagaa aatctgcatt gagtattgca taaaagccat
    18121 catgtcacct attcgaatac tgcctacggc tacctcatgg gaaccgaccc aaataatgga
    18181 tattgaaacc acattcataa tgatggtcat gaggggcatg agtactacca tagctctagt
    18241 gacatagagc atcatacttg tcaggtccct attagccttg tcaaagcgtt ttgcctcctg
    18301 atcttgcata ttgaagcttc taatgaccat catacctgat aggttttcgc gcacaacaag
    18361 gtttagtcta tccataagct tctgaactat tcggaatttg gggacaacaa gagcaaaaac
    18421 aagcccgatt atggcaagaa gagccccaac agccatcgcg atgatccacc acattccaga
    18481 ggccttcccc aaagcccgaa tgatgcctcc aattccgata atcggggcgt aaaaaagcat
    18541 acgcgtactc atcattatga gcatttggat ttgttgaatg tcgttggtag atcgcgtgat
    18601 aagcgaggct gtggaaaatg aatcgaattc tgcaagcgag aattcttcga cttttgagaa
    18661 taacgcattt ctgagatctc tcgatgtgcc cgcagataat ctcgaaccca ggaaaccaac
    18721 agataccgca gaacctacag tgataagcgt tatgagcaac atttgaatac caacacgcag
    18781 aatataggaa cgctgcattg caccgatatt ggctcccatt gcggtgaatt ctccctggag
    18841 cccaaggact ataatctgag attttataat aggatccagg gcttcgatct gtgccgaaag
    18901 ctcttgagga gtcatcgaag aaagaccgga gagatccgaa ctcgacatct gtggcgatgc
    18961 cttactcgct gccattgaac taaactgcaa aaacgcagca agttttggat attctgacag
    19021 aaaaaccttc cgagctgcat cgagctcagc tttgtcctcg cttgaaagca tcatgattga
    19081 ctctttctta gcgataggcc atttcttcga tagcgattgg gctttgggtg aatctggcaa
    19141 tacttcgata tagacctctt gcagagccgt aaaatcctct ggcctggctt ccatctgtag
    19201 aagcttttgc agtgcttcaa aagtgctctt tcgcatgaca gcaggtaaat cagcctcaat
    19261 accactttgt tgaatgccca catttactat tcttgatagg taatccggca aggatagatc
    19321 ggcattcgct tgaataaata gaaagaggat aatcaaaaca atattaagcc aataaggttt
    19381 tagaaaatga agaatagtcc gcatcttgtg ctcctattga aagcggatga tcagtttcaa
    19441 attattgcag gaaaggaaag aatcgatgct ttttcgggat tggtgaaccc accaaaaaga
    19501 caagcatatc tggtatccaa gatttttaac atattctcaa agcctcaaaa gaaaagatgt
    19561 gtaaataata agaaaagctc ctgttttagg caaggagaaa tgaagaattg tatgataggg
    19621 ttttttctat taccaaggct tgatttaacc cgacagttcg agttttaaaa gaatcttcct
    19681 ttataataac agacatttag cgcatctagt ctatgctcat gcaccctcaa gcgctggcta
    19741 aagtcttcaa aactgcgttt tccccgagga ctccaaaata cttccgagag ggcgcagaga
    19801 cgagggaaca gcatatactc cgcctgtctg ccaaaatata ttagttcact ccatagattg
    19861 gcttgcccac ccaaaatgtg atctgattcc tcatcgctca atttgtccga cacgggctcg
    19921 aatagatacg aatcgctcag cgtgcagact ccaagatgcc cgggttcttc tggttcatcg
    19981 ttatgcttat gatcaagata gcaagctctt gtctgaggac acataatcac atcataacca
    20041 cgcttagccg catggatacc cggttcgatt cctctccaag acattacgat ggtatctctt
    20101 ctgatatcgc catcgatgac ctcgtcccag ccaatcatcc ttctgccgta cttgaagagc
    20161 agattagata tttttcccat gaaccatgcc tgcaatttct cgggcaaata cccgctatct
    20221 tcgctcacga agcccagtgt attcattcgc tccctgcagt ctgaacattc cagccacctg
    20281 gtttttggcg cctcgtctcc gcccatgtgt acatatggac cagggaacat cttgcatacc
    20341 tcttggagca catcgtcaaa atagacaaac acatcatcat ttccggcaca aacaatatcc
    20401 tcgaaaattc catagcggtc ttcaggttca aagtgaacag ttgggtcagc tcttccttta
    20461 catgaatatt ctggatgaga tgcgaggaga gcgatgctat gacctggcaa ttctatctct
    20521 ggcaccacaa ggatgtgtct ggcctgtgca taatctataa tgcgttttac atcggattgg
    20581 ctatagtatc cacctttttt atattcttct tggtagcgca gatctctcct aaaagcccca
    20641 tactgcacaa gttcgggttt tgaaggaatc tcgagcctcc atgcctgatc gtccgtaagg
    20701 tgccaatgaa atacattgag cttgtggagg gctgcaaggt cgagaagttt ctcgatgaac
    20761 tcgaccctga aaaaattgcg cgcacaatcg agcatcaagc ctcgccagcc aaatcttggc
    20821 tcatctacaa ttttcccggc ttgaatcgac actccttgtt cttctttgcg gccatttcct
    20881 aaggcaagtt gccatagact tgccgcacct cggataatcc cagcactttc tgccgcttta
    20941 atctgaatgc aggatggcgc aatatccaaa atatagcctt ccggcgtatt gaaaaataat
    21001 tccggttcta gtgagagcag tatcatcgaa ccttcaggct tatttctttc atgtccactt
    21061 ggcatgaaca aagactcaga aatattcgaa tttgcagata cgattttaac tttttctatg
    21121 cctggaactc tggaaagcca ggccgcaagg acctctgttt ctttttccag cccaggtttt
    21181 gcaatcaacc atgtttcttt atcaaagcgg aaattgcctt ctctggactc ataggcaaca
    21241 ggaataggaa tgatactgta acggtcagac atctctcccc ctttagagac caaagcctgc
    21301 aatgagatcg cgataataat aaaaactcag tttggggcgt ctctcaagcg tattacgatc
    21361 tacggctaca atgccaaagg cacgattata gccaaaggac cattcaaaat tgtcgatcaa
    21421 actccataga aaatagcctt taagaggaac accttgcttt atagcatccc cacaggcatt
    21481 ggcgtggcct tttatgtagt ctattcggtc ataatcccgg acaattccat cagcatcttg
    21541 cttatccggg aatgctgcgc cattctcggt aacatatatc tttttgcagg gccatacatt
    21601 tgcgatatat gataatattc tgcgcaatcc tgatggctct atctcccatc ccatatcggt
    21661 cttcttctga taggtaggaa caaattcaaa tccatagggg ttctgggcgc tacagggagc
    21721 tgcgcgaaca gcttcttcat cataataatt tacaccaata aaatcgagag gctctgatat
    21781 tatatccatg tcgcccgcct caattggcat atcggagccg aatatgtcca taaaaaccct
    21841 gggataagac ctgccaaaga taggatcgag ccaaagcgaa gttcgctcaa tcgaagctcg
    21901 ctcggaagcc tcggcatctt caggccggag agttgccggc cttggctttg aaggattgat
    21961 gacaagacca tactcgccct tgattccgct ccgtctaaat gctcttaatg caagtccatg
    22021 cgctagaagc agatgatgca ctgctcggtt tgtcgcacta cgatctttta agcccggcgc
    22081 atgaatccct gttagatggc ctaagaatgc agcgcaccat ggctcattca tgcttgtcca
    22141 atggccaact ctatctccaa tatgatcata cataatgcct gcatattctt cgaagcgcat
    22201 cgctgtatcg cgattccgcc aaccaccttt ctcttctaat gcttgcggca gatcccaatg
    22261 aaacagctcg agccaaggct ctacacctgc ctcacatagg ccatcgacaa gtcgcgaata
    22321 ataatccagg cctttcttgt ttggtgcacc cttcccttcc ggttggatgc gagtccagga
    22381 aaccgaaaag cgataagctc cagcattgag ctccctcaga agtgcgatgt cttctttcca
    22441 tctatgatag gaatcgcagg ctatatctcc tgtatgattg cttaacactt tatctggcgt
    22501 atgggcaaaa gtatcccata tagagatccc ttttccatct tcttttgcgg ctccttcaat
    22561 ttgatagcaa gaggtcgaac aaccccatat aaaatcctga gggaaaccat cttctcgatt
    22621 catgcttgct cctcttttga ttagggactt atccttttat ggcacctgcg gccacaccct
    22681 ttgtaatttg ctttcggaaa atcatataga acacgagcat cggtatcatt ccgattgtaa
    22741 gggccgcaaa ttgctttcca taatcggaag aaagagaacc cgaaaattta tagataccca
    22801 ggggaagcga tttgagctca gtcttggaca ccaaaatatt aatcagcgca aattcattcc
    22861 atgtatttgt aatgttcaaa attgcaagag tcgtagcaac aggcacgctc attggcataa
    22921 taatactcaa gaatattctg aaaaacccag ctccatctat gcgagccgat tcgactaccg
    22981 cgcttggaat tgctctaata tactccgtgg acagatatat cccgattggc aacccggctc
    23041 caatatatac aagaagtacc gcaaaacgag tgttatatat acccaacaga ttcacttcaa
    23101 gaaaaagagg gatcatcaat gtctgggtag tcagaagaat ccccatgata aagctattgt
    23161 agataggctt tgttgctttg gacttgagtt tggcaaatgc aaaacctgcc aaaagtgata
    23221 gaaaaattat gccaagagtc gcccctagcg tatacaagat agaattggga aatagtaggc
    23281 taaaatctcc tatttcccat gctcctttat aattctggag ggtaggagat ttgggtagtg
    23341 caagctggct tatctgaaat tctctggtcg tcttgaacga attcataatg agccagaaga
    23401 tcggatagag cgtcataaag gtaaaaatga tcataatcag atagacagcg gctttgccaa
    23461 ttatggatag gatattcttg catttcgatt ccttgatcat ctcgggcatt gctcattcct
    23521 cctttccgcc atagcgcttt tctactgctt ttgtaagagc gatcagccca aaactgatca
    23581 ataccattat gagcgaaata gcgttggcta gtgggaaatt tggcctaccc aaaaacgcat
    23641 tgttatacat atagatggaa aggatctccg tgacacgcga aggccctccg ccagtcatgg
    23701 caaagataag agcaaaactg gacagcgagc ccgaaatggc taaaatcgcg gcattgacta
    23761 ttgtccccga caatgcgggc aaaatgatgt agcgcataac ctgcccttcg tttgccccat
    23821 cgatgcgagc cgactctacg acctgcacat cgatcttttg catgtttgcc aaaaaaagga
    23881 tgagatacat tcctgtgtac atccacagaa taacaaagag aatcggaagc atcgcgatat
    23941 ttggctttgt caagaaggca ggactccatt tttgaacaaa aagattgctg agcgcattat
    24001 agacctcctt gattccttca ccatggaatg aagacagaaa atcgcggaga tccacaagcg
    24061 gatccgagaa catcgcttgc ccagaaggcc ctacgagatc cagaattctc ttgactacat
    24121 catccgagag ggaaaaccca cccgcggcat caaatattcc tctaagttgg ctttgaaatt
    24181 ctctgaccct tatcgaattt ataacctcag ctattggccc atgtggtgaa aaaatcgttt
    24241 gccacaaaag gccaaccacg attacagaaa taatattggg cacataaagc acaccttgcc
    24301 agaattcagg cgccttgacg attttggcat agatgaaata agcaaaaatg aatcccagcg
    24361 gaagttgacc aaatacagaa atgagcacta tatagatatt attcttgaga gcattccaga
    24421 accagggatc acggaataca cgcagatagg catcgaatcc tgcgaatccc catttttcgc
    24481 ctccaaacat cttgccgcca gaataattgg ataccgatag cacaatagaa agaacaatag
    24541 ggaatgccat gaccaagaga tagatgccaa aggcgggcat aaccatagcc cagtaagccc
    24601 gtttttcttc tatataggat ttgctagaaa aggccatatg gactccccaa aaaggagcgg
    24661 aaagcgtaaa gacacaatgg ctcctccatg ctaaggcgtc tacgctttcc gacacctact
    24721 ttttcaatag tcttactttg atgaagcttt ccaggtttcg aaggctttct gaacatcggc
    24781 tgctactttc tccggcgttg ccgttccaag accgatggca attagcccat cattcagcac
    24841 attatagacg gccggatcga acaagccatc gaagacatag cataccttgg gattatctgc
    24901 atagaattgc ggtacttttg caacaagtgg ttcgacattg gcctttacgt tctttcgcgt
    24961 aggcacgtat gatcccgtgc tatagcgaat tgattgcact tcctcagaat agagatattt
    25021 gaatagtttt accgctgctt tctcccttgc ggatcctgaa ggaatagccg cagatattgc
    25081 ccagccagta cctgcaattc cagagacaac gccggggttc acttcacccg ggatacttgg
    25141 gaacgggaga agtacaaaat ctttctcctg tgaagccggc gggatgaggg ccttgcctga
    25201 agccttgtcc gtaatgtaag cacctgctct ccaatcgcca tcgatataga tcgccgcttt
    25261 tccggataca aagagaccgg gtccttcgcc atatccgacc tggttgtctt cgcgcgccat
    25321 gatcccatct ttatatagat tgtcgatgac ctttagcgaa tccacgaagg gcttgtccgt
    25381 gaacttggcc ttgccagcaa gaatggcatc gacgaagtca tcgccagcca tgcgcccaag
    25441 cacagtagag aagaggcaag attgcatcgg ccagccatcc ttattaggca gcattatcac
    25501 attgataccc ttggccttta gcttccatac cattctcttc agatcgtcgt aggtctttgg
    25561 taaatcgaag ccattgtcgg caagcaattt tttattgaca aacatgacag atgtataggt
    25621 gaaagcctgt ggcaacatgg ccagatattt tcctagctga ttattgggat cggtagccgc
    25681 accggaaaaa tccttgagga aatctggacc aagtagtttg gagaggtctt tagcgagctt
    25741 tttttcgtga ataattgcac ttctagatgt aggccataca tagaatacat ccggaagagt
    25801 gccagcagcc gcatagactt gcagtttctg gtgatatggc tcgctgaaaa gaacctcgat
    25861 attcaatttg atatccggat tatccttctg aaatttctcc cagatcgcga catcttcctg
    25921 atatcctgcc tggtctgcct gttggtagtt gagtacattt agcgtaacct tttcctgagc
    25981 caccaccgcg cctaacacta cgaaaagtgt tagtaacgag agtaaggctt ttttcataag
    26041 aacctcctta catcgggcaa ttctgcccgt gttccgatat gaaaaggcat atctgccttt
    26101 ttacccttgt taaatatgac taagaaatcc tgcctatatt atctgaagaa aaggatgaag
    26161 cgagccccgg tagacgatgt tcagacaata agcgctcaaa aaccatcccc gcagcgccaa
    26221 aagcaaccgc cttgtcacca aagctcgaat agcgaatttt cacatccttt ggcgaaggat
    26281 acatccagtt ttctttgagc cttcggcgca aaaccgtagg caaatccaca tcgagcgctt
    26341 ctatatttcc tcctatgaaa acttgctgaa aatccatggt gtttatgagc atggccatgt
    26401 tccgtgcaag ttcatccact gctctattga gcagacccct gtcgaatctc agcagcatga
    26461 gctcgttacg agtcaaggaa aattgcaggt ctcccggtcc attgcagaaa gcactacgga
    26521 attctccggt gtaaccatgg gctccagtat gtaccttccc tccaagaacg atcccaaatc
    26581 caacaccgat tcctccatat ttcaaaagag aagtctcatc ctttctatat tcaacaagcg
    26641 caaagagaaa atccttgagc tcttcaactc tattgaaagc gagctcaccc caagcacaac
    26701 agtttgcatc attctcgata aagcaaggga catccaattt gccttccatg gctttggcaa
    26761 aatccagtgt tctatcgatc cctagcggaa ccgagtatct aatgcgaccc ttcttcgaat
    26821 cgatcagacc tgcggtacct actccgacgc ctagaagaag ggaactatca cagcgttgct
    26881 tacgtgtctc ttcgattatt tgcagcacta cctgaacaaa attatcggag gtcacatgag
    26941 tatcgccccg tagagtagcc agaatattgc ccgatagatc cactattacc gcatcataga
    27001 atccgagctg aagctcgata cctataaaat gcccatattc tttatttgcc gaaagacaga
    27061 taggtttacg tcctcctcga gccgaagctt ctccttcttc ttgttccact atcaagccat
    27121 tttcgataag atgtgctact tgctttgtta ccgtggattt atcgagatcc agcttatgag
    27181 caagatcaat gcgacttatg gatggattgc gccatatggt gcgaaaaatc cttgccgctg
    27241 tgcggctcca ttgatctcca cgcggtggca tgccctatct cctcaacgtc agcctaaaag
    27301 ttctgctttt tcttccagca aaatgtaaat tcgacataat ttcataattg ttggttgatt
    27361 tatccaacca attaacatat aacaccggta tcttgaattg tcaacaaaaa tctttcagaa
    27421 tttggcgtga ggagaattct tttgggaggc aaaaagtgga agtgatcgat cttgcgggta
    27481 attggaatct tgtaaggtct tctgatacta caaatcgacc aatacatata cctggcgata
    27541 tttactcggc tctcatgatt tcgggagaat taccagatcc ttaccagggg gaaaacgaac
    27601 ttatggttca atggcctgga aaagaagact ggattatcga gcgatctttt ttcatatccg
    27661 aagcttttct ggcagcgtca agtataaggc tctgcgcaga tgtcctcgat acaatcgctg
    27721 aagtatatat aaataaagtt ctggtgggga ctggcaagaa tatgtttcgg cctttcgaag
    27781 ccgaagtccg tccggctctg catcctggcg aaaatgacat acaggtgatt atccgttcgc
    27841 ccgtccaggc ggctttgcgc gaatcacagc gcctaccata tccaataccc gcttcgatct
    27901 accctgtctc gtctccgcat cgcaatctaa tccgcaaggc tcaatgcatg tcgggatggg
    27961 attggggtcc ctgtctcatg actggcggaa tatacgatgg tattcgactt gtggcaacgg
    28021 atgggctatt tattcgctcc attcaatgcc atgcttcggc tctgggagac tttggaagcc
    28081 gtaatgattg cggcctggac ttttcgcttc aaatctcggt agagatcgaa gtgcccaagc
    28141 cgatgaaagt tgttgtctct tcctatatcg atggcaagaa agcgacagtg gaacaggagt
    28201 gcgaacaagg aaatacggta gtcagcacca acatggttat tcagaacccc agactatggt
    28261 ggccacatgg ctatggtgag gccgccctct atgatctggt tgttgacgct gctgtccaaa
    28321 cagccaaccc ccctcaacct catgtcatac ataaacgaat cggctttcga tcgctccagg
    28381 ttatttcaca agaagatagt attggcagga gcttcaagtt cctgataaat ggtaaagaaa
    28441 tatttgccaa gggcgcaaac tggattcccg cagatgctct cccgtcgcga tggacaaggg
    28501 cgcgcatcga gcatttgcta aattccgcca tagacgccaa tatgaactgc ctccgcgtct
    28561 ggggaggtgg acgctacgag agcgatgatt tttatgaact atgcgatcaa aaaggcattt
    28621 tgatctggca ggattgtatg ttttcctgcg cactctatcc ttccagccgg gagtttttgt
    28681 ccaatgtgga agccgagatc accgcacagg taaagagact ttcggatcat ccctgtatag
    28741 ctctctggtg cggaaacaat gaggcgcttg gcgctataac ctggtatgaa gattcgaaga
    28801 aaaatccggc tcgatatata gtcgattatg ataggcttac agaaggcgtt cttggaaacg
    28861 tgattcgaaa gctggatccc gaccgttgtt tctggccttc aagtccttct gctggacccg
    28921 acgatttctc tgacaactgg cactcagacg agcgtggaga tatgcatttc tggtctgtgt
    28981 ggcatgaggg caaaccattc tcggcatatc tcgatgtcca gccccggttt tgctcggaat
    29041 ttggttttca agccttgcca tgtatgtcga caatagctag cttcgctcat agagaagaat
    29101 ggaacatcgg tgcaccggca ctcgaagctc atcaaaagaa tcctcaggga aacagcataa
    29161 ttctagaaag cttgctgcga tactttcgca tgcctagcgg ttttcatgag ctgctctatc
    29221 tctctcaggt gcagcaagct attgctattt gcacggcggt ggaattctgg cgcagcacaa
    29281 tgcctcgatg catgggaaca ctatactggc aactgaacga tgtatggcct gcaataagct
    29341 ggtcgagcct caactatgat ggatcttgga aactcttgca ctacgaagca cgtagattct
    29401 acgcccctat cttgcttgcg ctctttatca aggatggaat aattcatgtt cgcctcgtga
    29461 gtgaaagccc taataatcat aaagccaaag tgtacataaa gctaatcgat ttttatgggc
    29521 gaccaatcga tatcccatcg aaagaacaag ggccaataat tgaacatgtt gtatctatcg
    29581 aagagaatca agtttatcag gtgtgggaga tgccgatcgt tgaattgccc tgcaaagcca
    29641 atcaagcctt tctcgaagcc acattagaag tccctgagat tggcctcttc tcgacctcga
    29701 ttctgttcct taccgaacct aagcgctgtg ccctgtcgga tccaaaactc gagacttcag
    29761 tctgctacga tgagaatcga gtgctcaaag caacaatcaa agccaccgat gcaccggcct
    29821 tcttcgtcat gccttacctc gaaggaatcg atgggcaatt cgatgattct ggtttttata
    29881 tgccaaaagg cagcaccaag attttaagct tttctccgat aaatgcggca gacgccactg
    29941 tcaagcaatc gcaacataag caatcgcaac atagccaaag gcttcgagca gcagaaaaga
    30001 ctttcatgcc tgcgcttcgg gtgtttcatt tgcagaacag cttttaactg cgcaaaaagc
    30061 gcataaaatc cttttatata agcatggttg cttcaatctc gtttctgata atttggtgct
    30121 tctttgctaa tgacgatgct atgtgggtgg ctttcggcat aagccgcggc tgtaatgcgt
    30181 atcattcgcg ccttccttag ttcggaaata gtccgagcgc ccgcatatcc catgcctgag
    30241 cgaaggcctc cgagcatctg caccatataa tcagagagct ttccgcgata tggaaccata
    30301 ccctccacac cttcgggaac tgccttacca ctctgtcgaa cgttcaactg cctatctgtc
    30361 gatgcatcag actggccaga accgtaacga tctcggccat atccttggag agctccgatc
    30421 gaacccatgc cacgatagct tttgaattga cgaccttcaa aaaacacaag ttctccaggg
    30481 gcttcctcga gacctgcaag gagattgcca agcatgacgg tctctgcgcc tgcggctatt
    30541 gcttttacga tatcgcctga atagcgaata cctccatccg ctatgattgg gataccatag
    30601 gccttcgcgg cttgtgcaca ctcatagatt gcggacaact gaggcatacc tacaccggag
    30661 ataatccttg tcgtacagat tgagccagcc ccgactccaa cttttatcgc cgaagctccg
    30721 gcttgaatca gcgcttgtgt gccctcggcg gttactacat tgccagcaat tacatggata
    30781 tcgccagaaa gagaccgaat gcgtcgaatt gcctcaatca cctttttcgt atggccatga
    30841 gcggtatcga tgacaagcac atctacattg cgagatagaa gcaagctgac tcgctcctcc
    30901 aaatcagaac caacgcctac ggcggcacca acgagaagtc tgccacgcgc atccaccgct
    30961 gcattcggat actcttcttt cttgacgata tcttttaggg tgatgagccc tttgagcctg
    31021 ccttcttcgt cgactaaagg gagtttttcg attttgtaac gccgaaaaat cgccttagcc
    31081 tcttcgatac tagtcccttc gggagcggtt atgaggtttt tggaggtcat gaattccgat
    31141 actggttttg gcatatcctg tgggccacag aaacgcttat cacgattggt gattatgccc
    31201 actaagcgac cagtcaatgg atcaacaacg ggaattccag agattcggta ggtctccatt
    31261 agatgctcag cttctgcaag tgtcgcagtt tccggaagcc acacagggtc ggagatcatg
    31321 cctgattccg aacgcttgac cttgtacacc tctgcagcct gagcttctgg cgataggttg
    31381 cgatggacta ttccgatgcc gcctatacga gcaatggcta tggctagcct ggaatcggta
    31441 acggtatcca tggccgcgga aagcaccgga gccttaaggc gaattccagg gacaagctcc
    31501 gcggagatat cgacttctga aggaagaacc tcgctatatc taggcacgat caagacatcg
    31561 tcgaatgtaa gacctgcgcc ttcttgcaga agtttttcaa gattcatttt ctacaacctc
    31621 tcttaagata gttctttcgt gtccaatatg gctctagtgc cggaaatggc gaactccggt
    31681 atagaccatg gtaattccaa gcttgtcggc cgcggcgagc acctcggcgt ctcgcacgga
    31741 gccgcccggt tgaaccaccg ctgtcacgcc cgcccccgcc gcggcctcaa tgccatcggc
    31801 aaatgggaaa tatgcgtccg aagctagtac cgaacccctg gcacggtcag cggcgcgctc
    31861 gcaagcctgc cgtactgcgt cgaccctgtt tgtctgtccc ccacctatac ccaccgccgc
    31921 cctgcccgcc gccagcacta ttgcgttaga cctgacgtgc ctcaccagct tccaggcgaa
    31981 tcgcattgcc tcccattcgg ctgcggtggg cttcctgtcg ctaaccacac tccattttgt
    32041 gttcatgggg tcgccgcgat cctgttcctg aaccaaaatg ccgccagccg cagacctgag
    32101 ctcgcgcgcc tctttttcaa ataaaagagg atcagcggct ataagcctga ggtttttttt
    32161 cgcttttaga atttcacggg cagcttcatc gaagtcaggc gctgctatgc actccaaaaa
    32221 gagccccttc atcataaggg cggtttcgag atccagggga cggttcagag caacaatgct
    32281 accaaatgca ctcactgggt cgcaggcaag ggctgctctg tatgcttcct gcagactatc
    32341 ggcctcggca gcaccacaag gcgagagatg cttgacaatc accgcggcag ggccttcgaa
    32401 atcgagaacc gctcgccagg ctgcatctag atccagcaga ttattgtagg aaagctcttt
    32461 gccttctaat acctgcccac ctaaaggccc gcggataatg ctgcggatcg atcgtgtctt
    32521 gggcagaaca aaaaaagcct tctgatgagg attttcgcca taacgcagcg tacgctccac
    32581 tctgcctaca agcgaaaaaa cgccgcccgg ctcttcattg cccaactcag cgccaaatga
    32641 ggtcaatccg gcagccggca aggcaaattc atcggaattt aggctttcag cattgctctc
    32701 taaaaaccat gctgcaatcg cagcatcata atctctggtt cgagcaaagg cctttgctgc
    32761 gagtgctctc aaagtagatg atgaaagacc tcctttttct atttcgcatg caacaaggtc
    32821 ataatctaac ggatcacaca aggctcctac ccttgcatga ttcttcgccg cagcacgcag
    32881 cagggcgaca cctccgatat cgatctgttc aatgatttcg gattcatcaa gcgaggcgct
    32941 cggcacaatc tcagccgctt cggcatccat agtattatta gcgattggag ccgtatttgc
    33001 atttatagta atagcatcag aaaccattcc ttcatcaata tttggcacct ttgtatcggc
    33061 caacgatcca gatggtttta ttttccccct tttttcagca agcgcttttt tacccatctt
    33121 aagccttcgc accgtctctt cgaaaggata gaggttcacc actacgagat cgatgggttc
    33181 ataaccatga gcatgcaatt cctggagatc atcctctgat gatcgtgcaa gaatgccggc
    33241 atggattgct ggatggagag tcttaactct tccgcccaat agctccggag aaccagtata
    33301 agccgcgata ttcaatggtt ctagttttgc ctctctgaga gttgcggcag ttcctcctga
    33361 ggcaagaaag cgccaaccac aggctgcaag ccgctttgca aaaggcacaa gtcctgtttt
    33421 gtcatatacc gatatcaatg cgataggcat gccctgactc cttttctccc aatttatctt
    33481 gatctcttgt ttcttgcatc tcttggcttt gaatctcttt aggccttggg ttattagcaa
    33541 gccgccgtgc aagatttatg agttctcgat gctcgacact gtgtattcgt tcttccagaa
    33601 tgctcaatgt gtcgccttta ttgataggca catgtacaac actcagcacc ggcccggaat
    33661 cgataccctc atccggcaca aaatgcagca tgacgcctgt atttgcgatt cttccttgat
    33721 taaaggcttc aaaagccctt tctattgcgt tggtaccggg gaatgccccc ggtaaagcgg
    33781 ggtgaaggtt tatgactctg aatggaaaat tctccagaaa agtccgtccc aaaagtcgca
    33841 tccatcccaa cagaaaaata aaatcaggct tgtaagaatt tgctactgcc gcaagcctct
    33901 catcatagca cctacgctca ggagaagaac gctttgcctt ttgcggagcc ggcaaaatat
    33961 gcgaaggaat accatggcaa atcgcccttt cgatacaatg agcccctggt ttgtcacata
    34021 gaagagcctc aatacgcata ttctctatcc agcctgattc gatagcatcg attagagcct
    34081 gcaaattgct cccttctccc gaagccatga cgacaatgcg cacaatgcta tctgaaactc
    34141 gattaccatc gtttttggtc atcatacgag aaccacattc cctctacccg agactagtct
    34201 tcctatcaca aaggcttgtt caggcagcat attcaagaaa gagttgagct ggctttcttc
    34261 cactatcgca accataccga tccccagatt aaagacccta cgcatctctt catctggcac
    34321 attcccccag cgttgtaaaa gcaaaaaaag cgctggccat cgccaactct ccgcatatat
    34381 ttgcgcatcg agataagggg gaagcacacg gggaatgttc tcgatcagcc cgcctccggt
    34441 gatatgtgcc agagctttaa tcggacatgg gtttgcattg agaattgctg ctaacaaagg
    34501 aagatacgat cgatggggcc gcaaaagtgc cgatgcaagg ctttcgccca gttccgggta
    34561 tactctttca agttccatcg aagaagtaat cgatcgtgcc agggaataac cattggtatg
    34621 aagccctgaa gaggctaatc caatcagaac atcccctatc gctatatcgg atcggggaag
    34681 aagcttatct ctttcgacga tccctacgat tgtccccgcg atgtccattt catcctcgcg
    34741 gtatgtccct ggcatttcgg ctgtttctcc tcctagtagc acacaatctg cttgtcggca
    34801 agcttcggac attccttcga caatctttgc gacctttgcc gggtcgatct tatccgcggc
    34861 tatgtaatcc ataaagaaga gaggacgggc accctgcacc aggatgtcgt taatcgagtg
    34921 gttgactatg tcagcaccaa gcccggtgag ccaaatgccc ttattctctt cggacccgtt
    34981 attccttttg tctttaggct caccgcgctt atgtttacca tgactcagtc tatcgagctt
    35041 aagagcaagg ctagtcttgg tacctactcc atcggtagag gcaacgagca ctggcgcttt
    35101 gcaatctttt acaagtaaag catcatattg gcctccgaag gccccaattc ctgcgattac
    35161 tctggaatta taggtcgatc gtacggctct cttcattagc tcaacagcca tattgccggc
    35221 atcgatatcg acgcccgctc gcacataagc gcttgatgga ttgctttcct cgacttgagc
    35281 atagacgctt gattcttttg tccctgccga aagcgctcgc tttacagccc actcgaccct
    35341 ttcggcctta gagccgatgt cgcggcgaaa ttgcttgcct tcgaaatgga tctgcgaaac
    35401 agcctcataa gctgctttaa gcgcttcggc ccgcgctgaa cctattccca acaccgaaac
    35461 caccctacct ccggagctta caagtatccc attttcaagt cttgttcccg catgcaaaca
    35521 ataagccttc tcgggcagat ctcctatcgt tatgggcttt ccttttacag gttgatcggg
    35581 atatcctaga gaggctaaaa ctacacaagc ggcatgttct gccttaaatc gcggtcttgc
    35641 atcacccaat cttccatgag cgcaagccga caacgaaatc aggacatcgg aatcgaaaag
    35701 cggtagaatc acctgagttt caggatcacc taaacgggca ttatattcca gcacttttgc
    35761 cccttcagcc gtaagcataa ggcctgcata gagcgcccca atatacggat atccctcttc
    35821 atagagtcca gaaagcgcag gagcgattgt tgcacaagcc attctttcgg catcttccat
    35881 agaacaggcc ggagaatagg ctcccattcc tccggtatta ggtcctttat cttcatccag
    35941 aaggcgttta tgatctctag ccgtaggcaa taccacatag tctttgccgt cgcagagagc
    36001 gatcagagag agctcttcac cttccaggcg ctcttcaagg acgatttctc cttcggctgc
    36061 taatcgctct atagcggcct cggtctcttt ccaattctcg ggtacaatca cacccttgcc
    36121 agcggccaaa ccagaagcct tgaccaccag aggtctacca aaatccttag cccaggcttt
    36181 tgcttcctct ggatttgcga aacttttgcc ttccgcggta ggaatgccat gcctcatcat
    36241 aaaagccttg gcaaaagctt tggaactctc gatttgcgct gct
//
LOCUS       DAOOWY010000009        35724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1223_length_35724_cov_9.234461, whole genome shotgun sequence.
ACCESSION   DAOOWY010000009 DAOOWY010000000
VERSION     DAOOWY010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 35724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35724
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1223_length_35724_cov_9.234461"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /gene="secG"
                     /locus_tag="PK105_01525"
     CDS             <1..332
                     /gene="secG"
                     /locus_tag="PK105_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOU60415.1"
                     /translation="VIIQDEESDSIGGIFASGAQSAFGSRTSNIVVRITYALGALFFV
                     IAFSLAILNKSSTGNIQKVVEQESAQTATSEWWNSQSEEDQSVQGQLAPQDQTVQPEG
                     TNTPATK"
     gene            477..875
                     /locus_tag="PK105_01530"
     CDS             477..875
                     /locus_tag="PK105_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60416.1"
                     /translation="MRIGINAIGSRKLSSVSNAMLHSLKQKGHDSVLTSDYSYSLGSF
                     DFLIFVTEPKGMFGGLLSSVPQDLAKSGDLVGKRCLAMVQKRGFRSGHTLKKLMEALE
                     HEGLVIVQGEVFSNTDTAIHIIAEVPLKRG"
     gene            879..1733
                     /locus_tag="PK105_01535"
     CDS             879..1733
                     /locus_tag="PK105_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOU60417.1"
                     /translation="MKNYYEILGVEPDSSPKDIKSAFRRQAKRLHPDMFYSKEKARSE
                     ESTARLRESAMRLILEAYKILSDAEKRRSYDRELRRQEKENKGFDYREFLKMRADDPQ
                     SQARLIVFDLLHGFEEEALWIYERSKGFQDFRLERWLERGEAMDCEYCIAEEYEKRGK
                     YIKAYQIYKKLIQMELEKPWFRYYFDVVALQFRLLVLQKLPGKIDDDDYLDRLEEAIE
                     LGISPRETAQYLRKKVEILIHRGEADRAAEALLQISQIYPKLAGFDSLRVKVERALGQ
                     QVVQDRVY"
     gene            1786..2817
                     /locus_tag="PK105_01540"
     CDS             1786..2817
                     /locus_tag="PK105_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU60418.1"
                     /translation="MKRIATWFCIFLFTAISVFAQTSIDKPAATIKLIKQEVISVRQL
                     RTDVEKLENSVGVKLTLDQRKDVLDARINSMLFLQFCEREKISISEAQVNAALAQLRS
                     QLGTNATDADLEKSLRASGVFVDPATFVRQRLLFETYIQTKRQDELKIALKAPTADEI
                     LKAYDLTKASLIRPDTIRISVIYVDTKGKSNGEIAKAKELMNSISASLKSNPSKFDEY
                     VLRASDQAGYKAIPSMYLEKTQQSKTIFGDEFFNVAFQANVGEITPVIETPVGYRIIR
                     VNEFLAQKQLTLSDPVPGNQNLTVQEFLAIQLASEKQQAFLNKAEADLIEVLRKEATI
                     KIYSENLNW"
     gene            2830..3267
                     /gene="nusB"
                     /locus_tag="PK105_01545"
     CDS             2830..3267
                     /gene="nusB"
                     /locus_tag="PK105_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOU60419.1"
                     /translation="MASRRKARILAVQAIYAWDMSHQELEDILSFEWLDEDKKARYEI
                     EILDFAKLLIIGTIEQIDSIDDLIKNHLQHWAFERLRKVDLAILRVGTYSLIYQQDVP
                     AQISIDEAIEIAKEYGSDDSYRFINGVLDGIRKDFFGEFHDSN"
     gene            3254..4000
                     /gene="map"
                     /locus_tag="PK105_01550"
     CDS             3254..4000
                     /gene="map"
                     /locus_tag="PK105_01550"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOU60420.1"
                     /translation="MIRIKNERQIEGIRSSCDLLSLLYERLKPQVQAGNTPKDLDRFA
                     YDFIIKHGGKPAFLGYEGYPATLCISVNEVVIHGIPNDKPFQEGDIVGIDSGINLNGF
                     FSDAAITLPVGSIAQEAAKLLKVTRESLELAIERLKPHVRISEISRAVFSHATKNGFN
                     VVRQYCGHGVGLEIHEDPQIPNYVSPGPNPRLMSGMVLAIEPMINIGTSDVRVLDDDW
                     TVVTMDGSLSAHFEHTVLITDSGHEVLTRW"
     gene            4118..5629
                     /locus_tag="PK105_01555"
     CDS             4118..5629
                     /locus_tag="PK105_01555"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOU60421.1"
                     /translation="MSLRKRLYSRNFFVANLVILGIVVGFGLAFLFRAPPSGNSSLPV
                     VKAETPSMVTNPDTEASIETAELVQNALHNIAKTVLPAVVELDVVEGGQQTQQQAPSF
                     PFYFFFGPDSPFSDQFQPQEGLGSGVIVRRTGKTVYILTNHHVAGNAAKITVKLSDGR
                     EYEGKLVGSDERKDIALVKFETDDSDISIAKLGDSSKLQVGDLAIAVGSPFGYIASVT
                     AGIISALGRTGGPDENINDFIQTDAAINKGNSGGALVNIKGEVIGINTWIASPSGGSV
                     GLGFSIPINNVKDAIDDLITNGTVKYGWLGVSLSNISQDKASAKELGLEGKKGAFISH
                     IFLNGPADKAGILPGDFITAVNGKAIQSTDELVRVVGDIKPGSTAKLEFIRSGVVMTA
                     STKIELRDNAIASNNANIFPGVTVISLKSDSIDQKKIPSGVRGALVIDVLAKSPAASM
                     GLQSGDIITKLNGKNIDNLRDFYELLAKESEKKLVFTVNRNGQTVETLAYVRK"
     gene            5714..6346
                     /locus_tag="PK105_01560"
     CDS             5714..6346
                     /locus_tag="PK105_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOU60422.1"
                     /translation="MHKEFVTYDTVRNNAIKLAYRIYRDGFIPDIIYVSLRGGAYMGN
                     VISEYFKFVKDNEHPVFYAAVVARSYTGFDQQESIKIDGWTYNPDFLRHGDKVLLVDD
                     VFDSGRTINYLARVIMERGLPRSDVKIAVHDYKVREYVPQHLPILPDYWCRKIEVKSR
                     EDELWIHYMSHELEGLTQQEIAEHYLKDDPSLAEPMSFVAKESTSSTSDK"
     gene            complement(6358..7632)
                     /locus_tag="PK105_01565"
     CDS             complement(6358..7632)
                     /locus_tag="PK105_01565"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOU60423.1"
                     /translation="MAEILSIVLGGGKGTRLFPLTKERAKPAVPFGGKYRIVDIPLSN
                     CINSDFRKIYILTQFNSASLHLHIYRSYNFDRFSKGFVEILAAEQTHLHSGWYEGTAD
                     AVRKNLIHFRPHQPTHYLIVSGDQLYRMDLGEMFKQHVESGSRLTVAGTLVDRDTATS
                     LGIIKIKENRSIDSFLEKPGPTKDIHEYEVPKSLRPEGASPNKPYLASMGIYIFDAPL
                     IEETMQLDANDFGKEIIPFVVSKEKVNTYVFDGYWEDIGTVRSFYEANIGLTDINPKF
                     NFYDEQSPIYTHARNLPASKLNYCTLNQALASDGCIITNASITRSIVGIRTVIESGAS
                     LDGVVCMGSDYYENAEEKEENRRKNIPNIGIGGGSIIRHAIIDKNARIGSNCRIGIDP
                     LARQEGEYRTYYIVDGIIVIPKNQIVPDGTVI"
     gene            7696..10011
                     /locus_tag="PK105_01570"
     CDS             7696..10011
                     /locus_tag="PK105_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU60424.1"
                     /translation="MNSESANAIYSEFLSWLAERNEIAWSSILPPKSADCMPFPEDLS
                     PILSEALHRRGIDSLYTHQRKSYDLVRSGHDIVIVTPTASGKTLCYNLPILQTLLEMP
                     KARALYLFPTKALAQDQQSGLNEVMLAGSIGFPVNTYDGDTPSDIRSRARTDGRIIIS
                     NPDMLHAGILPNHTKWVKFFSNLKFIVMDELHAYRGVFGSHVASVIRRLLRIAKFYGS
                     SPTFIFSSATIGNPQELAEHYLERKVEMIEDNGAGSGEKILVCINPPIVDEVQGLRRS
                     NALEAESAMLWLLQKGVRTILFSRSRLQVELLAHYLNQKLENPFNRNYGIVVKPYRSG
                     LLPSERREIERGLRNGTVHGVVSTNALELGIDIGGLDAAVITGYPGSIASFWQQAGRA
                     GRTSGISLAIYVASSSPLDQYFVAHPEYFLSRGAEQAHIDTYNPYIFTDHLKCAAFEL
                     PFSEYEAFSPDNSNRRAAELTIEALECLEEEGIVRHTEGRFFWSAEGYPGEKISLRSA
                     TTENVVIVDISNGHHDVIGEMDRPSAKELLFEKAIYMHLGSQYQVKKLDFEKRICLVE
                     ASDADYWTDSIVKRDIALLTEDSVESYSQFRLILGDILVRSQVEKYKKLRFNTHENIG
                     YGEIWQEPEEMQTRSLMMVIYPQSQSAQFLSQFGQESANSLLYGIANLMRQLAPAKIL
                     CDVRDIGVASRARDPFYESPVLYLYDMYPGGTGIAEALCSNMSGIIGSTIERIASCAC
                     LVGCPSCIGVENANPDNKAASLRLLELLSCS"
     gene            10086..11375
                     /locus_tag="PK105_01575"
     CDS             10086..11375
                     /locus_tag="PK105_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOU60425.1"
                     /translation="MNLKERLALIKSNEKNNKGNSAPRPRKMPDNWRNILPYVWIREI
                     QGPIFIVPPFFYSGIIRSARPSERFSEQTSETEGDIDSQGSAFRIPADRVIFFDLETT
                     GLSGGAGTYAFLSTTARFEGRNLVLNQVFLEDYPGERDFLSMVISQLSAGDWIASYNG
                     ATFDIPLLRSRCILNGIVMPIRMHVDVLHDCRRFWGKILDSCSLASMEKFVLKSTREF
                     DIPGSEIPRVWLDYVKSDFLSENQKALMELVWQHNIMDVVSLARLFLHIESLYRDPYR
                     AVIEDSVDPLSLANRICKLGRLEEAKSLLLMIYRNNKEHDLSREIIREVQRYLAKLAR
                     KDKDLDLFSELVLSMDSEFLYGCVAKAKLFEHTFKDEKTALVWAQKAHDLACNSVNSG
                     TIKRKDKEMAAQLSVIASLDHRIARLERKIANRKSIP"
     gene            complement(11301..12401)
                     /gene="rlmN"
                     /locus_tag="PK105_01580"
     CDS             complement(11301..12401)
                     /gene="rlmN"
                     /locus_tag="PK105_01580"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOU60426.1"
                     /translation="MNYDSADRLSAVYPSGLSQEELAAILSEEPLYRAKQAFAWFSRG
                     VSSFEKMTNLPVALRERLAQNFGGSVRSSVVQNEFSDKDGSLKLQIRLRDGASVECVL
                     LEDIEGRKTACLSSQVGCAMGCAFCKTGTLGFLRNLGPDEIVEQFHHLRERRGFISNV
                     VFMGMGEPLLNLENVRKSISILSHPAGIGLSMRKITISTSGIVPAILDLAKNGPNVRL
                     AVSLTSANPDLRTELMPVNRKWPLDDLKSALLEYQQDTNARITFEVVLLGGLNVGEKD
                     AIELVNWITPIKSQVNLIAWNPIDGMPFKTPSVRQIEEFSRILSDNGIVTVRRMSRGR
                     GVMAACGQLGDTLSICNLALEPSNTMIKRGND"
     gene            complement(12398..13534)
                     /locus_tag="PK105_01585"
     CDS             complement(12398..13534)
                     /locus_tag="PK105_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU60427.1"
                     /translation="MPSLPYNDDFFEIPNEIQLENTEVAEISKKGYQLLKENRFDEAV
                     ECFVQILEKDKENNYALVGLGDAARKQERFKEAADYYRRCLVFHPGNSYALFGLADCY
                     KALNQFQKAIEIWEQYLLHDNTNITVLTRVADAYRKVRDFRKSKSIYHRVLELNPDNH
                     YALIGLGHLHYDFKEYKDALSYWQRMVELEGDNVDIRVLTAIGNCYRKLKHFDKGIPI
                     FEKALQKEPDNFYALFGIADCYRGVGKQNMSLLYWNKILERDPKNKVILTRAGDAYRN
                     MGDFEKATEYYQNALNIEFDIYAVLGLAVIARQQGKYDEAIASLRSLLQNDPKNYRIY
                     VELAQTYLLNNQRQMAIEILTEFQQMGIKNPVIQDALFKLTAKT"
     gene            complement(13709..14245)
                     /gene="rsmD"
                     /locus_tag="PK105_01590"
     CDS             complement(13709..14245)
                     /gene="rsmD"
                     /locus_tag="PK105_01590"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOU60428.1"
                     /translation="MRITGGEYSGRIVKVPEGDLRIRPSMDRMRQSVFDVLGDLLGYS
                     FLDLFAGSGILGLEAASRGASPVVCVEKDRKKFPVLLQNAAMAKPAVFCKAMPAETFI
                     MRNKQAFDIIFLDPPFDYRFKLQLLERLDRSSSIHDGSLVLIHFPREDNLPQEISGLR
                     CYDMRIFGRSIVHFYRRI"
     gene            complement(14251..14718)
                     /gene="smpB"
                     /locus_tag="PK105_01595"
     CDS             complement(14251..14718)
                     /gene="smpB"
                     /locus_tag="PK105_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOU60429.1"
                     /translation="MKTQDSIKIIAINRRARFDYEIEERLECGIALYGSEVKSIKEGR
                     VSFSDAFAEIKGREVWLNNLHIADYPHSSIFRHDPDRAKKLLLHRQEIKRLERRVREK
                     GFTLIPLSIYLKHGLIKLELGLCRGKKAYDKRAEIKARDLDREIRREFKVKDW"
     gene            complement(14715..16268)
                     /locus_tag="PK105_01600"
     CDS             complement(14715..16268)
                     /locus_tag="PK105_01600"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU60430.1"
                     /translation="MKESEKITDQDIEKAKVTLKPIFGIRPRIYIPILFAVLIFIILF
                     FLLVNPGLVNPGAKISFEGNPDVAALYAENTYIGNTSDGIRLRPGSYQIEIRKRGFEP
                     KTIAVKVPNRIFATLILPPRVTIQYELKSESLESILIPSFKEFASWSLSGRPSAIYQL
                     PMVLSEASKDLAAFPVADTVPIAEPLLAAGLSIAQNTSSIRDLIYASFSLGAPGASPL
                     GFISMARSAIALLANSKSYVGAFSEIAPERTDDAIRNALSALEKETSLFETSLYNPVG
                     VQIIGSHSFIIFNEGYLKGLTSTPGGTGIPYIALVPKFGLASTEVTQRQYARFLKENP
                     EWAPENRATLIEKGLVDNYYLADIDISSPSDKPITGVSWYAANAYCEWLCGYAPGGYE
                     VRLPTEAMWEMAASQAYRNIKNLGVFNNRSSNGPLSVASYGRDASGFSDLFGNVWEWT
                     SDSFRPYVWITEDSPAFKMLANAGNSKTVKGGSWANSADQISIESRGPMPADHSSEFL
                     GFRPALIRK"
     gene            16347..17312
                     /gene="lepB"
                     /locus_tag="PK105_01605"
     CDS             16347..17312
                     /gene="lepB"
                     /locus_tag="PK105_01605"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOU60431.1"
                     /translation="MDILDKLVVFSEDLLTRRKKRSLRAKMKQQKRHPILDWIYAILW
                     AACVVLVINQYFFQNYRIPSGSMEKTLLVGDMIFVDKLSYGPELLPGVLKTPGVSTPK
                     RGNVIVFENPTYLSRGPIYTIFHQLLYMVTFTLVDIDREPTGEPRVHYLIKRAIGVGG
                     DTLRVRNGEVFIRPRGSSVFFDERMLMDGLGLPAKMQRLVESSEYPEIDNVGVASAYT
                     ELGLSLPSRVGMPNVQHANKDAFQYDMVRVTTLRDANPSDYRNAQLAQRYENGWYIAD
                     SRLFPMGDNRDNSRDARYFGPVAEKKVLGHALFIYFPLSRIGSIH"
     gene            17325..17906
                     /locus_tag="PK105_01610"
     CDS             17325..17906
                     /locus_tag="PK105_01610"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOU60432.1"
                     /translation="MFAKRSIRYAERIQRKRTRNRILLYIALFIVLCIVLRTFVFQTW
                     KVRGELMKPTLRDGDIVIVLPCLSFSDKMLVSLAGIPQKGDVVLVEDGSESIVPTMTM
                     IEDSALRFMTLQKYSLLAAEFGKGFGLPTVMRIQSIQHDKDISKSQPVYRLATDCQSD
                     KNFMTGDRIVPPSLIKGRVVLKIWPLARIGKVQ"
     gene            17903..19162
                     /gene="hemW"
                     /locus_tag="PK105_01615"
     CDS             17903..19162
                     /gene="hemW"
                     /locus_tag="PK105_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOU60433.1"
                     /translation="MKAWTSQVFGHERGIFPIIDPFKIRSLYVHIPFCADRCSYCDFF
                     SVPLIKDPTFSTIEYEISYVQATLMNIRNWSKLFGAGPFSTIYLGGGTPSVIDRRALK
                     ILIDDLEQFVDTDCEWTIEANPDSLDKALLDMLAGTGVNRISLGIQTLSEEEWPILRR
                     VGSIDEAVQAIELLCEYPFELSVDLLMGIPNKTNKGRQGHSSIMNSLEYLIGRVSHLS
                     IYDLTLEKGTLIESQVSSGKLIMPDSDELAEERDKVDLYLEGQGFHRYEVSNYAKPGH
                     ECRHNAVYWNMEPYLGVGSGGVSTIQYPMCEESQELGTIIRITETRDISAYIEAPMEI
                     PKDIEIIDKKTALFEFLMMGFRTSRGIDLKRIKSLFGIDVEECIPSALKRWNSEIVRN
                     GDFVALRPDNFDILNRFLVECLEELDL"
     gene            19172..20431
                     /locus_tag="PK105_01620"
     CDS             19172..20431
                     /locus_tag="PK105_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60434.1"
                     /translation="MAIFQYAVISSILHDEPQKKQLLSVFEDKLQRFGGELHTLESNM
                     IHPRNSELPLFMLVLTGGTESEAMSLISRERMLDLRMPIVLLAHPSHNSLPACLEILA
                     KVKQEGGKGLILQADSSGAFDAEELEDIIAVCKSRITMRSCQIGVIGRPSDWLIASSQ
                     DLHAAMNSWGVRLVEIPLEELVRNIEEIRSRSCGVPVIEDFQEKAEFYAESSNDDILK
                     SIQILQALRILVDKYRLSSLTLRCFDLILKDRSTGCLALSQLADEGIDAGCEGDIPSI
                     IGLSWIRSLTGQIGWMANPSHIIAKKGEDSAEMLIAHCTAPRSILSRYALRSHFESGL
                     GVAISGILPKEEVTLFRIGGKYLDKLWYSPARIIASPRDEGLCRTQALINLPNEKAQE
                     LLINPLGNHIVMVRGNWTRRISISAQL"
     gene            complement(20447..21055)
                     /locus_tag="PK105_01625"
     CDS             complement(20447..21055)
                     /locus_tag="PK105_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU60435.1"
                     /translation="MKIYHHYSVYGFSNIYTIVNESLKKAIIVDPSEITPTMIERIEQ
                     NELDLCGILVTHDHIHHVNGLSTIMKIYSPTIYASDAKLLGFQCRKLRDGDAFSEAGF
                     DITAIAVPGHSQDSIVFLMEGYFLFTGDVLHAGVIGKTNSVFNTNALIARIKQKLFCL
                     PESTLIFPGHGPPSTIGTEKSANISFQEGFAERIRPNYDFFV"
     gene            21885..22079
                     /locus_tag="PK105_01630"
     CDS             21885..22079
                     /locus_tag="PK105_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60436.1"
                     /translation="MCRFYTPGAHWDCRETIEELVQDKEKRNHCEFFMVSEKYLEKGE
                     TADSKQDDDAKRRFNALFGE"
     gene            22101..22898
                     /locus_tag="PK105_01635"
     CDS             22101..22898
                     /locus_tag="PK105_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOU60437.1"
                     /translation="MRICVFDSGIGGLPFVQAVNREFPFLEIDYVADDAGFPYGTKSP
                     EMIRDILFERFRRIRARIDPEVLILSCMTAVQIGLKELQAAHRTLQIIGAIPPIAEAA
                     NESQTHRIALLATSRTTEDSFLDYLIARDAPDCEVIRIPAQDLIEYAEQRLPFASKLS
                     AQTMVAPYINYALKEGTDRIVLASSQFILLEDSITNQLNELQAFNVKCLDPRGKVICS
                     LQKLIPDKEHSEGQFVKPRFYLTGDQGANPSLIAWSERFNCSKPQLL"
     gene            22874..23902
                     /gene="rsgA"
                     /locus_tag="PK105_01640"
     CDS             22874..23902
                     /gene="rsgA"
                     /locus_tag="PK105_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOU60438.1"
                     /translation="MFQTTASMNGNSKESTVLGVVVSFSNRNAKVRCSDGNILTCSLK
                     GKITDSSFQGYKSPVAGDHVSVIPISKQNGVIESIFERKSELSRYNEKGETKQVLAAN
                     IDLVVCIASTRAPPFRARFIDRVGVMAEYCGLPFLIVCNKIDLGLDVSIESRLAAFQK
                     LGYGVVKSSVRDSIGIDELRTSLLGKTSVFIGQSGTGKSSIINCLIPGADQRIAEISD
                     KHKRGKHTTTLSAMVSSSNGDIDIIDTPGIRRLAIRNIEPDDLGFYFPDMVPFLGLCE
                     FGASCTHRYELHCFVKQAVQEGIIHHDRYESYLRMRAELEETRNTKTNESRKPLRSAT
                     YKFEDEEW"
     gene            23896..26388
                     /locus_tag="PK105_01645"
     CDS             23896..26388
                     /locus_tag="PK105_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOU60439.1"
                     /translation="MVTNQHSLALLDFYRLRDRLEEYCFSHEGRALLNSTLPCSQENS
                     VKRLKKDLSLLVEGLNEKEIPSFPFPNIENPIIKAAKEGTVLEAEELFAIGLWVKSYH
                     QLFSFLRRILDSFIEESKEVSAENSMDGEIRQEDSINDESWAEWGIENIVYSAPALSQ
                     VTQMIFAVITEDGEIKDLPRLKALNLAIQRTQAENLHIINRYLSDPSLQDALQSNIAT
                     ERDNRVVIAIKANFKGRIKGIVHEFSSTGQTIFIEPIELVERNNKLLELESKLNEEIR
                     AILRETTAALRHFLPILMRARGFLAAADARFARAIQVKREGLAIAGHLEHGIHLYQAK
                     HPLLGKKVVPIDVDIPDDSRILIITGPNTGGKTVSLKTIGLLALLNQYGAGIPALSTS
                     AFAVFDTILADIGDEQSIDQSLSTFSGHMKVISEIISSATDRSLVLLDELGAGTDPEE
                     GCAIAMALLDYLYRLQCFAIVSTHHGVLKNYGYTKPGCLNASMEFDAESLSPTYKMIM
                     GVPGESRALEIAGQIGLPMEIVDIAKSYLEDERSDYSMIIHSLGEKQRELEKLELEKK
                     KQLGKVLELRRKVDLENLRIRRRELELRRQGIAELKHFINESRRIFENIVRELRENGK
                     KDGDTSAGREFLKEISDEIHRQSETLESFAQETNRIERSLKPEMQGNIGIDIEKMKFG
                     DTVIFHNREAVFLRKLDKKNALVQIGSLRVPIEVESLSLPKTMDRFDKAAKPGYQIEL
                     ASDSMGRNAPVAELDVRGMRLSEALDLLEHQIDAASLAGVSNFSIIHGTGEGILSRGI
                     HEWLKKQISVADYYFARPEDGGFGKTWIHLKE"
     gene            26536..27270
                     /locus_tag="PK105_01650"
     CDS             26536..27270
                     /locus_tag="PK105_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU60440.1"
                     /translation="MSGHSKWASIKHKKGALDAKRGQLFTKLIKEISIAARMGGGDIE
                     GNPRLRTAVLKARAANMPKDNIDRAIKKGTGELEGSIYEELFYEAYTQGGGAMLIEVL
                     TDNKNRAAAEIRNIITRSGANLGTTGSVAYLFKRKGILTYDSEKYTEDQIMEAALEAG
                     ADDVVSEDGTISVYTDPSSFEDVLNVMNDKGFETLGAEISMLPDSYITVDLETAQKIQ
                     KLIDKLEENEDVQSVYHNIEMPELEE"
     gene            27279..27785
                     /gene="ruvC"
                     /locus_tag="PK105_01655"
     CDS             27279..27785
                     /gene="ruvC"
                     /locus_tag="PK105_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOU60441.1"
                     /translation="MNSRSSLVAIGIDPGIAAVGYGLVRTLEGRLSLVTYGCIETESD
                     EPIGKRLTYIFEEIRKLLGQYAPDIGGIEELYFFRNVSSAFPVAEARGVIRLAFEKTG
                     IALKEYSPNAIKKAVTGSSRASKSQVQEMVKILLGMSQIPTPDHASDALAAAICTLHS
                     IGPAGYTL"
     gene            27787..28392
                     /gene="ruvA"
                     /locus_tag="PK105_01660"
     CDS             27787..28392
                     /gene="ruvA"
                     /locus_tag="PK105_01660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOU60442.1"
                     /translation="MFNRIKGVFSGHEDEALHISTGALEWEILVPIRDPSFYNHTGEE
                     IELFTWLHHYEDAMRLYGFFSALERKLFLDLQKVEGIGPRQAFKILQGIAPRSLVTML
                     DSGDLASLQKIPGIGPKTAQKMMLALKGKLITQEEASAVISGSQGRFRDVVRALVEMG
                     FERKEVERVTAQLASSLEDSPEAEKELFKRALLELSAGAGA"
     gene            28431..29435
                     /gene="ruvB"
                     /locus_tag="PK105_01665"
     CDS             28431..29435
                     /gene="ruvB"
                     /locus_tag="PK105_01665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOU60443.1"
                     /translation="MNPLYNPAEDDKEASLRPRYLAEFQGQQRIKDNLLIFIKAARDR
                     GEALDHLFLMGPPGLGKTTLALVVASEMGVDFKPTSAPAIEKPKDLAGILTSLRPGGV
                     LFIDEIHRLKPSIEEMLYIAMEDFELDWIIGQGPAARTVRIPIPQFTLVGATTKAGMV
                     SAPLSSRFGISLRFDYYSPQELASVIRRSSNILDIKINEDAIELLSGTSRGTPRIANR
                     LLRRMRDFAQVRGEGIITRDITLDGLERLEVDYLGLERLDREILKTIILKYGGGPVGA
                     ETLAVSVSESIESLEDYYEPYLIQIGMLARTPRGRVATQTAYRHLGIERPNENGQFRF
                     "
     gene            29410..30519
                     /gene="queA"
                     /locus_tag="PK105_01670"
     CDS             29410..30519
                     /gene="queA"
                     /locus_tag="PK105_01670"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOU60444.1"
                     /translation="MKTDSFDFDLPEHLIAQYPTEQRGRSRLLVLDRKTGNTIDSMVE
                     DIVSFIEPGTLMVFNDSKVRKARLYGTCEQTGTIVEFVFLSPWKQDDPTSLGDSSSDH
                     ESQGDLVKACKPISTVSRLWRAICNKSKQQKIGRSYMFPGQLRGSIIEDRGHEKTIEF
                     SFIVGDEYFDLYGHVPLPPYIKREDTSDDELRYQTVYAKHTGSAASPTAGLHFTPQLL
                     DELSRKGIEIEYLTLHIGLGTFQPVRADNVEDHQMHEEWFDISLEAADKINLALSEGR
                     KVLAVGTTSMRALESAWYGGKVKHGNFSTRIFIYPGYEFHVVDSLFTNFHTPKSTLLM
                     LVSAFAGREKILECYRLAIEKNYRFFSYGDAMLIL"
     gene            complement(30582..31223)
                     /gene="tmk"
                     /locus_tag="PK105_01675"
     CDS             complement(30582..31223)
                     /gene="tmk"
                     /locus_tag="PK105_01675"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOU60445.1"
                     /translation="MPTYNTDELKNRFIVIEGIDGAGTTTQVRLLGETLIARGFSCMV
                     TAEPTMNHIGQLIRTILSGEDMVVPTTVAHLFAADRNEHLYGKNGIMESLKSGSLVIS
                     DRYVLSSLAYQGTTCGHRLPWLLNSAFPAPGLTLLFEVDPEICISRIRSRLKKEIYET
                     LEFQRRVQTMYESMAYRLSNKGWRIERIDGSKDIESVQRQIESIVFAFLGFEC"
     gene            31277..>33765
                     /locus_tag="PK105_01680"
     CDS             31277..>33765
                     /locus_tag="PK105_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60446.1"
                     /translation="MRTKKPVIVIVIFSLALAIWGSIVIADLVQREVQMLAIKALDRL
                     QSAVVDATGLILKYDNLDIESLNRFSMHGIALIRSDAAEHNGNKPSSYSEFYPSSERS
                     NYEEQPPLRIKKLSIRISIWALFSGHNNEAVRDITVDDVQISLYLPNDEYIIKKILEN
                     FVSGKAESVPHFELSIGPVRAEVREIAKSSNASMDNNVINEGSLTIESLQFSSLTDSP
                     VIIAPSVILKTSGLLGLPKDLNAEFGLRGSGNANLSDIDMNIDLEASSTGWHIMPQKL
                     LLEKRGKRIECKKLGGGIELSGSYEGGVWGIEGVFEGYGVGEDIELGGEWGEIVKGSE
                     VSGEIGVGGDLEGLSRYEVDLGIDIGVGISIGGYDLGALGVSLIGVGDAVSYIGGIRV
                     EVGGNEFRYEGEVGYEGIRLGGNYELSGVDIGIGGYIRGGIGEYEVGVGKGELKGVEI
                     GLGVCRVRVGVEGYEVRYEGAVGGGEVKVGGSIGKAGGYEGVVELEGVGISEVMGALG
                     VGGIEGEVSGRVYGLERGGKRSWSISGLAYDGEVMGAEVSLRGDGVGDEAGYEIKVLE
                     ASIGDYDIALRGKGSYVGELFSGEFSAGGMSYEVSVEARGGKVRVGIGEGFRGAFELV
                     GGRLEGEVGMDKFGLGLGEGMIRVGGLASGWYEAGGWGISLEGMSLSYEGEGKYPEVK
                     VSGLIGDSGIRFEWLEVKGDGYGFKGSIDLKGEGGEYILIGSGKGEGVLGEGSEYSVY
                     GVIRGKGVDLRVGVQGLGVGSYVVEGYADLRGEVDIREVLKGSLEGIGDIRGEVAVGL
                     SGSGLRVDEQRFEVEKRGKRIECKKLGGGIE"
     assembly_gap    33766..33865
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33866..>35724
                     /locus_tag="PK105_01685"
     CDS             <33866..>35724
                     /locus_tag="PK105_01685"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOU60447.1"
                     /translation="YEGEGKYPEVKVSGLIGEQGADLDVDYLQYAGQSLKGSMIIQYS
                     SIANLLKDTKIDYSFLDIKTDRTILEGGIVALDDKLEVAIKTSSIPLEEVFPSTFNIS
                     GDIELKGSSTISILSKKLDLSALSSLNLQFNTRKSEMKGIPFSAAGTINFKDCTFSLE
                     SGIFSYQNYRFENINAEYNLESKQVAYALNSKIVIGEQLLSANLKGFGDNIDSSIFKL
                     GAAQAFHITGEINDASLGSKTIDPLSYSISNADDKLDIHLSQANGDSIDASITNMTDF
                     DLVLNDLFKIKGRANGKIQAKEVWADIALEKIDLKTLGALISKDDLGDTSGIASAELN
                     LSGDISDPRIEGKILFSNLIIDQNLYIVEKIGPFDAEIAINDGVAELSPTIINIGTGD
                     ISLAATANLARWQLSDITVLVSTLEKASMRFKGKIGGLNTDGIWIKGDIKAAISSNSI
                     EIGGDMLLDDGTLEVDPNGFISSENAQTPVSPLYLKLMVTFGKNVQMYIPGKDISLIR
                     GTISPASTFTVQYDQARGDFAVNGRIELRSGYVYYLLRDFFIKKCTIELAESQSKFNP
                     VMDVIAELMEPTKDGMIKIILSADKSPLDNFSPVLSSVPPKSQIELLALLGGG"
BASE COUNT        10299 a         7059 c         8499 g         9767 t
ORIGIN      
        1 ttgtgattat ccaagatgaa gaatctgatt cgataggggg gatttttgca agcggggctc
       61 agagtgcctt cggttctagg acatcgaata tcgtggtccg gataacctat gcgcttggcg
      121 cgctattctt cgtaattgca ttttcccttg cgatattgaa taaatcttcg acaggtaata
      181 tccaaaaggt agtggagcaa gagtctgccc agacagcaac cagcgaatgg tggaattccc
      241 agagcgagga agatcagtcg gtccagggtc agctcgcgcc gcaggaccag acggtccagc
      301 ctgaggggac taatacacct gccactaaat agctctattc agctcaattt gaaaggcagg
      361 aagctaaagg agggcacgga agaatgtaat tccgtgcttt attctatgaa taattagcat
      421 gaaatagcgt attctatgcg taacttgcta attcatatag atgtttttta attattatgc
      481 gaattggaat aaatgctatt ggatcaagaa aactctcttc agtttctaat gcaatgttgc
      541 attcattaaa acagaaaggt catgattcag tactgacatc agattattcc tatagcctcg
      601 gctcctttga tttccttatc tttgtaacag aaccaaaggg tatgtttggt ggtcttctgt
      661 cctcagttcc tcaggatttg gcaaaatcag gagatttagt gggaaaacgc tgccttgcta
      721 tggtgcaaaa gcgaggattc agatcaggcc atacccttaa aaaattaatg gaagcactgg
      781 agcatgaagg cttggtgatt gtacaaggcg aagtcttttc taatacagat actgcgattc
      841 atataatcgc ggaggttcct ctgaaacgcg gatgagccat gaaaaactat tatgagatac
      901 ttggtgtaga gccggattca agccccaagg atataaaaag cgcatttcga agacaagcaa
      961 agcgtctcca tccagatatg ttttattcca aagagaaagc cagatccgag gaatcaaccg
     1021 caaggctccg cgaatcggct atgaggctta ttcttgaggc atataagata ttatccgatg
     1081 cagaaaagag aagaagctac gatagagagc ttcgaaggca ggagaaggag aacaaaggat
     1141 ttgattatcg cgagttcctt aaaatgcgag cagacgatcc tcaaagccag gcaagactga
     1201 tagtctttga ccttctgcat ggtttcgagg aggaggcact atggatctat gaaaggagta
     1261 agggctttca agatttccga cttgagcgtt ggctcgagcg cggcgaagcc atggattgcg
     1321 agtactgcat tgcagaggaa tatgaaaaac gaggcaaata cataaaggct taccagatct
     1381 acaagaaact aattcagatg gagctggaaa agccatggtt tcggtattac tttgatgttg
     1441 tcgctcttca attcaggttg cttgtcttgc agaaattgcc tggtaaaata gatgatgatg
     1501 actatctgga caggctcgaa gaagctatcg aactagggat ttcgccaaga gagactgcgc
     1561 aatatcttag aaaaaaagtc gaaatcttaa ttcatagagg agaggcagac agagccgcag
     1621 aagctctgct gcaaatatcg cagatctatc ctaagcttgc tggctttgat tcattgagag
     1681 taaaagttga gcgtgctctt gggcagcagg ttgtacagga tagagtatac tgaacagcac
     1741 ggtatagtgc ttgtaaataa ataatattga gagcaggcta atgatatgaa gagaatagca
     1801 acttggtttt gtatctttct gtttactgcg atttcggttt ttgcgcaaac gagcatcgat
     1861 aaaccagcgg caacaataaa actcataaag caagaggtta tttcggttcg ccagttgcgc
     1921 accgatgttg aaaagctgga gaattccgta ggtgtgaagt tgacattaga tcaaagaaag
     1981 gatgttctcg atgcgagaat aaatagcatg ctgtttttgc agttctgcga gcgagaaaaa
     2041 atctcgatat ctgaggctca ggtaaatgct gcattagcac agctcagatc tcagttgggt
     2101 acaaacgcca cagatgctga ccttgagaaa tcacttcgcg cttccggcgt atttgtagat
     2161 cctgcaacct ttgttcgtca aagactgctt ttcgagactt atattcagac caagagacaa
     2221 gatgagctaa agattgcgct caaggcgcca acggcggatg aaatcttgaa agcctatgat
     2281 ctgacaaagg cttcgcttat aagaccggat acgattcgaa tcagcgtgat ctatgtagat
     2341 actaagggga aaagcaatgg tgagattgca aaagcaaaag agcttatgaa ctcaatctct
     2401 gcctctctca aatctaatcc ctcaaagttc gacgaatatg tgcttcgcgc aagcgatcag
     2461 gctggataca aggctattcc ctctatgtat cttgaaaaaa ctcagcagag caaaacgata
     2521 tttggcgatg aattcttcaa tgtggccttt caagcaaatg ttggtgaaat aactcccgtt
     2581 atcgaaacgc cggttgggta caggattatc cgtgtcaacg aattcttggc tcagaagcaa
     2641 cttacactct cagatcctgt tccgggaaat cagaatttga ctgtgcaaga gttcttagcg
     2701 attcaacttg cctccgagaa acagcaagct tttttgaaca aggctgaagc ggatctgatc
     2761 gaggtgctgc gcaaagaagc aactatcaaa atatattcgg aaaacctaaa ttggtaaggg
     2821 acatttctgg tggcttcgag aagaaaagca agaattcttg cagtacaggc catatatgcc
     2881 tgggatatgt ctcaccaaga acttgaggat atattatctt ttgagtggct cgacgaggat
     2941 aaaaaagcac gctatgagat cgagattctt gattttgcta aattgttgat tattgggact
     3001 attgagcaaa tcgattctat cgatgattta ataaaaaacc atcttcaaca ttgggcattc
     3061 gagagacttc gaaaagttga tctggccatt ctaagggttg gaacttacag cctgatatat
     3121 caacaggatg ttccggcaca gatcagcatc gatgaagcga ttgaaatcgc taaggaatat
     3181 ggtagcgatg actcttatcg ttttatcaat ggtgtgctag atggcataag aaaggacttt
     3241 ttcggagaat tccatgattc gaattaaaaa tgaacggcag atcgagggaa ttcgatcgtc
     3301 atgtgacttg ctgtctttgc tctatgagag gctgaagcct caggtacagg ctggtaatac
     3361 gcccaaggat ttggacagat ttgcctatga ttttattata aaacatggag gtaagcctgc
     3421 ttttcttggt tatgagggct atcctgctac cctctgcatt tctgtcaatg aggtggtcat
     3481 tcatggcata cctaacgata agcctttcca agaaggcgat attgtcggca tagatagtgg
     3541 tataaatctg aatggttttt tctccgatgc tgctattaca ttgcctgttg gatccatagc
     3601 tcaggaagcg gctaaattgc taaaagtaac aagagaaagc cttgagctcg caatagaaag
     3661 actaaaacct catgtgcgca tttctgagat ctctagagcg gttttttccc atgcaacgaa
     3721 gaatgggttc aacgtggtac gacaatattg cggtcatggg gttggactgg aaatccatga
     3781 agatcctcag attccaaact atgtctcgcc tggccctaat ccaaggctaa tgtcgggaat
     3841 ggtgctggca atcgagccaa tgatcaacat aggcacaagc gatgtacgtg tactagacga
     3901 cgattggacc gtcgtaacga tggatggttc gctatcagct cattttgaac atactgttct
     3961 cataactgat tcaggacacg aagttcttac aagatggtaa tattctttca tttctttttc
     4021 taatttttta tctcttgtac agttcatggg agagtgcagt tttattatat ttgatacaga
     4081 ttgcaaatac tgtgccaaat aacggaggat atcgaacatg tctttgagga aaagattata
     4141 ttcacggaat tttttcgtcg cgaatcttgt aatacttggg attgtggtag gatttggcct
     4201 tgcattttta tttagggcgc ccccctcagg aaactcttct cttccagtag tcaaagctga
     4261 gaccccatcc atggtgacca atcctgacac tgaagcttca attgaaactg cggaattagt
     4321 gcagaatgcg ctgcataaca tcgcaaagac agtacttcct gcggtagtag agctcgatgt
     4381 ggttgagggt ggtcagcaga cccagcagca ggcgccatca tttcctttct atttcttctt
     4441 tggacccgat agtcctttct ccgatcagtt tcagccccaa gaaggcctcg gctctggtgt
     4501 aatagtgcgt agaactggca agacagtcta tattttgaca aatcatcatg tagcaggaaa
     4561 tgcagcaaag attacggtaa aattgagcga tggccgcgaa tacgaaggta agcttgttgg
     4621 atccgatgag cggaaggata tagccctcgt aaaattcgag acagatgatt ccgacatctc
     4681 gattgcaaag cttggcgatt cctcaaaact acaagttgga gacttggcaa tagcggtagg
     4741 tagccccttc ggttatatcg cttcagttac agccggcatt atcagcgcat tgggcaggac
     4801 cggaggtccg gatgagaata tcaatgattt tattcaaacc gatgccgcaa ttaacaaagg
     4861 taattccggt ggggctcttg taaacatcaa gggggaagta ataggcataa atacgtggat
     4921 tgcatctcca agcggaggat cggttggcct cggtttttct attcctatca ataacgtaaa
     4981 agatgcaatc gatgacctta tcacaaatgg cactgtcaaa tatggctggc tgggtgtaag
     5041 tctctccaat atctctcaag acaaggccag tgccaaagaa ctaggtcttg aaggcaaaaa
     5101 gggtgctttc ataagccaca tttttctcaa tggtccagca gataaggctg gaatcttgcc
     5161 tggagatttc attacggccg tcaatggcaa ggctattcag agcaccgatg agctggtaag
     5221 agtggtaggc gatatcaaac ctggttccac cgctaaattg gaattcatcc gaagcggggt
     5281 tgtaatgacg gcgagcacaa aaatcgagct tagggacaat gcaatcgcat cgaacaatgc
     5341 taatatattc ccaggtgtaa ctgttatctc actcaaatcg gactctatcg atcagaaaaa
     5401 aatacccagc ggagtacgag gggctctcgt tatcgatgtt cttgcaaaat ctccagcggc
     5461 atcgatgggt ctgcagagtg gggatattat aactaagctc aatggcaaga acattgacaa
     5521 cctcagggac ttctatgaac tgctagctaa agaatccgag aaaaagctag tcttcactgt
     5581 caatcggaat gggcagacag tcgagaccct tgcttatgta aggaagtaag tatatatttc
     5641 ttttctagag aaagtcattc gataagtgaa gcctccataa gggggcttca cttgtttttt
     5701 cggaagggat tctatgcata aagaattcgt aacttatgat acagttcgaa ataatgccat
     5761 caagctggct tataggatct atagagatgg cttcatacca gacataatct atgtttctct
     5821 caggggtgga gcttacatgg gcaatgtgat aagcgagtat ttcaagtttg taaaagataa
     5881 tgagcatccc gttttttatg ctgcagtcgt tgcaaggtca tatactggtt ttgatcaaca
     5941 ggaatcaatc aagatcgacg gatggactta taatccggat ttcttgaggc atggcgataa
     6001 ggtgctgctc gtcgatgatg ttttcgactc aggccgcacc atcaactatc tagctagggt
     6061 cataatggag cgaggcttgc caagaagcga tgtgaagata gctgttcacg actataaggt
     6121 aagagagtat gtccctcagc acttgcctat cctcccagat tattggtgta gaaaaatcga
     6181 agtgaagtcc agggaggatg agctttggat acattatatg agccatgagc tagaagggct
     6241 aacccagcag gaaatagcgg agcattatct caaagatgat cccagccttg cagagccgat
     6301 gagttttgtt gctaaagaga gcactagttc aactagcgat aaatgagtcg ctaacaatta
     6361 aattactgta ccatcaggga ctatttgatt ctttggaatg acaattattc cgtccactat
     6421 ataataagtt ctatattcac cttcttggcg tgcaagagga tctattccaa tacggcagtt
     6481 cgagccaata cgagcattct tatctattat tgcgtggcgt atgatagagc cgcctccgat
     6541 acctatatta gggatatttt ttctgcgatt ttcctctttt tcttctgcat tctcgtagta
     6601 gtctgagccc atgcacacaa ccccatcgag gctcgccccc gattcaataa cagtgcgaat
     6661 gcccacaatt gaacgagtaa tcgacgcgtt agttattatg caaccatcgc tggccagagc
     6721 ttgattcaaa gtgcaatagt tgagtttaga agcaggcagg ttacgcgcat gagtatatat
     6781 tggcgattgc tcatcataga aattgaattt cggatttata tctgtaagcc cgatattagc
     6841 ttcatagaag ctacgaactg ttccaatatc ttcccaatag ccatcgaaga catatgtatt
     6901 gactttttct tttgaaacca caaatggaat aatctccttg ccaaaatcat tcgcgtccag
     6961 ctgcatggtc tcttctatca gaggcgcatc gaatatatat atccccatgc ttgccagata
     7021 tggtttatta gggctggctc cttcaggtct aaggcttttt ggtacctcat attcatggat
     7081 atccttagtg gggccgggtt tttccagaaa actatcaatg ctcctatttt cttttatctt
     7141 aataatgcca agggaggtgg ctgtatctcg atcaaccagg gtccccgcta ccgtaaggcg
     7201 actgccggac tcgacatgtt gtttgaacat ctctcctaaa tccatacggt aaagctgatc
     7261 acccgagacg attagatagt gagtcggctg gtgaggccgg aagtgaataa ggttctttct
     7321 aaccgcatca gcagttcctt cataccatcc agaatgcaaa tgagtctgct cagcagccag
     7381 aatctcaaca aaaccctttg agaagcggtc aaaattgtag gaacgatata tatgaagatg
     7441 aagggatgcg gagttgaatt gagtgagaat atagatcttt ctaaaatccg aattgatgca
     7501 gttggataag ggtatatcga caattcggta tttccctcca aatggaaccg caggcttagc
     7561 tctctccttt gtgaggggaa atagacgagt tcccttgccg ccccctagca cgatagaaag
     7621 aatttctgcc atatatgctc ctttcttgtc agctgccgat ttaccttttt tattttgatg
     7681 attacagtat aacttatgaa ttctgaatcc gcaaatgcaa tttatagtga attcttatcg
     7741 tggctagcag aaaggaatga gattgcctgg tcatccatac tgccgccaaa aagtgccgat
     7801 tgcatgccat ttccagaaga tttatctcct atactaagcg aagcgctcca tagaagaggt
     7861 atagatagtc tctacacaca ccaacgaaag tcctatgatt tggttcgtag tggacacgat
     7921 atagttatag taacaccgac agcctccgga aagacgctct gttacaattt gcccatactc
     7981 cagactctcc ttgagatgcc aaaagccaga gcactgtatc ttttccccac aaaggcgcta
     8041 gctcaagacc agcagagcgg actcaatgaa gtaatgcttg ccggaagtat cggtttccct
     8101 gtcaatacct acgatgggga tactccgtcg gatattcgct ctagagcaag aacagatgga
     8161 cgtataatca tttccaaccc agatatgctt catgctggca tactacctaa ccatacaaaa
     8221 tgggtcaaat tcttttctaa tctgaagttc atcgttatgg atgagcttca tgcctatcga
     8281 ggtgtctttg gaagccatgt cgcttcggtc ataagacgat tacttcgaat tgcgaaattc
     8341 tatggatctt ccccgacttt catcttttca tcggcgacaa tcggaaatcc acaggaatta
     8401 gctgagcatt atctagagcg gaaagttgag atgatcgaag ataacggagc gggaagcgga
     8461 gaaaaaatcc tagtctgcat caatccccct attgtggacg aagtgcaggg cttaaggcgt
     8521 tcgaatgctc tcgaagctga atcggcaatg ctatggctat tacaaaaggg tgtgcggacg
     8581 atcctctttt ccagatcacg ccttcaagta gagctgcttg cgcattatct aaatcagaaa
     8641 cttgaaaatc catttaaccg caactatggc attgtggtta aaccctatcg atcgggtttg
     8701 ctcccatcgg aacgcaggga aatagaaagg ggcttgagaa atggaactgt tcatggagtt
     8761 gtctcgacaa acgctctgga gcttggcatc gatataggtg ggctcgatgc cgcagtaata
     8821 actggttatc ctggaagcat cgcttcattt tggcaacaag caggtagggc aggtcgaact
     8881 tcgggcatat ctctggctat ttatgtggct tcttcttcac ccctagatca gtattttgta
     8941 gctcatccgg aatattttct ttcaagggga gcggaacaag ctcatatcga tacatataat
     9001 ccttatatat tcaccgatca tctcaagtgt gctgcttttg agctaccctt ttctgaatat
     9061 gaggcatttt ctcccgataa ttctaatcga agagctgccg aactgactat cgaagcgctc
     9121 gaatgtctgg aggaagaagg tatagtcagg catactgaag gcagattctt ctggtccgcg
     9181 gaaggttatc caggggaaaa aatatccctt cgttccgcta cgacggagaa tgtcgttata
     9241 gtagacataa gcaatgggca tcatgatgtt attggagaaa tggatcggcc aagtgcaaaa
     9301 gagttgctct ttgaaaaagc aatatatatg cacctgggca gtcaatacca ggtaaagaag
     9361 cttgatttcg agaagcgcat ttgtcttgtc gaggcaagcg atgctgatta ttggactgat
     9421 tcgattgtaa agcgcgatat cgcattgctt actgaggatt cggttgaatc gtatagccag
     9481 tttcgcctta tacttggaga tattcttgtg cgaagccagg ttgaaaagta taagaaattg
     9541 cgatttaaca cgcatgaaaa tattggatat ggagagatat ggcaagagcc ggaagaaatg
     9601 cagactcgtt ctcttatgat ggtcatctat cctcagagcc agagtgctca atttttaagc
     9661 cagttcgggc aagaatctgc taattcttta ttatatggta ttgcaaattt gatgcgccag
     9721 ctcgccccag ccaagattct ctgcgatgtc cgcgatatcg gagttgcctc tcgtgcaaga
     9781 gacccatttt atgagagtcc cgttctctat ctatatgata tgtatcccgg tggcacagga
     9841 attgcggagg cgctctgctc gaacatgagt gggattattg gttctacaat cgagaggatt
     9901 gccagttgtg cgtgccttgt tggctgtcct tcctgtatag gtgtagaaaa tgcaaatcca
     9961 gataataaag cagcatccct aaggctatta gagttgctct cgtgctcttg aattatttga
    10021 atcatctcca gctgaaatat atcgagcaaa gtcggccaaa gggcaggcaa caaggagctc
    10081 agtaagtgaa tcttaaagaa cgccttgcat tgattaaaag caacgaaaag aataataaag
    10141 gcaactcagc tccaagacca agaaaaatgc cggataattg gcggaatatc ttgccttatg
    10201 tatggataag agagattcaa gggcctatat tcatcgttcc tcctttcttt tattcaggca
    10261 ttattcgatc agcacggcct tctgagaggt tttctgagca gacttctgag actgaaggag
    10321 acatcgattc tcaaggctcg gcatttcgaa ttcctgccga tagagtgatt tttttcgacc
    10381 tagagactac gggactctct ggcggtgcag gaacttatgc ctttctttcg acaaccgctc
    10441 gctttgaagg aaggaatcta gttctcaacc aggtatttct ggaagattat ccgggagagc
    10501 gcgattttct ttcaatggtg atttcgcagc tttcggctgg agactggatt gcaagctata
    10561 atggagctac attcgatatt ccgcttctca ggtcgcggtg catactaaat gggattgtta
    10621 tgccgatcag aatgcatgtc gatgttcttc acgactgccg acgattctgg ggcaagattc
    10681 tcgattcttg ttctcttgcc tctatggaaa aattcgttct taaaagcaca agggaatttg
    10741 atattccagg atctgaaatt ccaagagtct ggctcgatta tgtaaaaagc gattttctaa
    10801 gtgagaacca aaaggcgctc atggaactgg tgtggcagca taatattatg gatgtggttt
    10861 cactagccag gctctttcta catatcgaat cattgtatcg cgatccatac agggctgtta
    10921 tcgaggattc agtggatccg ttaagtttag ccaatcgaat ctgtaagcta ggaagactgg
    10981 aggaggctaa atcgctactc ctgatgatct ataggaacaa taaagaacat gatttatcga
    11041 gggaaataat aagagaagta caacgatatc tcgcaaagct agcgaggaaa gacaaggact
    11101 tggatttatt ctcagagcta gtgctttcta tggatagcga attcctatat ggttgtgtgg
    11161 ctaaagcaaa actcttcgaa catacattca aggatgaaaa gactgctcta gtttgggctc
    11221 agaaagctca tgatttggct tgcaattcag taaactcagg cacaatcaaa agaaaggata
    11281 aggaaatggc cgcccagctt tcagtcattg cctctcttga tcatcgtatt gctcggctcg
    11341 agcgcaagat tgcaaataga aagagtatcc cctagctgtc cacaagctgc cattacgccg
    11401 cggccacggc tcatacgtcg aaccgtcaca attccattat ccgaaaggat ccgagaaaat
    11461 tcttcgatct gacgaacact gggtgttttg aaaggcattc catctatagg attccaggca
    11521 attagattta cctgagattt gatcggggta atccaattta caagctcgat tgcatctttt
    11581 tctcccacat tcaggcctcc gaggagaacg acttcaaatg taatacgtgc attagtgtct
    11641 tgctgatatt cgagaagcgc cgatttgaga tcatcgagag gccatttccg attgacaggc
    11701 atgagctctg ttctaaggtc agggttagcg gatgttagag atacagccag acgcacatta
    11761 gggccattct ttgcaaggtc caaaattgca ggaacaatac cactcgttga tatggttatt
    11821 tttctcatcg acaatccgat gcccgctggg tgcgatagaa tagaaatact ttttctaaca
    11881 ttttcgagat tgagaagcgg ctctcccatg cccataaata cgacattcga tataaatcca
    11941 cggcgctccc gaagatgatg aaattgttcc acgatttcgt caggtccgag atttcgaaga
    12001 aatcccaatg tgccggtctt gcagaatgcg catcccatgg cacagccaac ttggctggaa
    12061 aggcaggctg tctttcttcc ttcgatatct tcaagaagca cacactctac agaggctcca
    12121 tcgcggagtc ttatctgtag cttaagagag ccgtctttat cagaaaactc attctgaact
    12181 accgatgagc ggacactacc accaaaattt tgagccaatc tttctcttag agccactggg
    12241 agatttgtca ttttttcgaa agatgacact cctctagaaa accatgcaaa tgcttgtttt
    12301 gctctataaa gaggctcttc agataagata gcagccagtt cttcctgaga taggccgctc
    12361 ggatagactg cactcagtct atcagcggaa tcgtaattca tgtcttggct gtaagtttga
    12421 acaaagcatc ctgaataacc gggtttttta tacccatctg ctggaattcg gtgagtattt
    12481 cgattgccat ttggcgctga ttgttcaaga gataggtctg agctagctcg acatagatac
    12541 gatagttctt tgggtcgttc tgcaagagcg agcgcaggct ggcaatagct tcatcatact
    12601 tgccttgctg tcgagcaatc accgctagac ctagtactgc ataaatgtcg aactcgatat
    12661 tgagggcatt ttggtagtat tctgtggcct tttcaaaatc acccatattc cgatatgcat
    12721 ccccggctcg ggtcaggata actttgttct ttggatctct ttccaggatc ttgttccaat
    12781 agagcagtga catattttgt ttgccgactc ctctatagca atccgctatg ccaaaaagag
    12841 cgtagaagtt atccggctct ttttgcaggg ctttttcgaa aatcgggata cctttgtcga
    12901 aatgcttaag cttgcgataa caattaccaa tcgcagtcaa aactcgaatg tcaacgttat
    12961 cgccttccaa ttcgaccatg cgctgccagt atgagagcgc atctttgtac tccttgaaat
    13021 catagtgcag gtgcccaagg cctataaggg cataatgatt atctggattg agttcgagaa
    13081 cgcgatgata aattgatttg gatttccgga agtcgcgaac cttgcgatat gcatcagcaa
    13141 cgcgtgtcaa tacggtgata ttcgtgttgt catggagaag gtattgttcc catatttcaa
    13201 tggccttttg gaattggttg agagccttgt agcagtccgc aagcccgaat aatgcatagc
    13261 tattacctgg atgaaaaacc aagcaacgtc tataatagtc tgcggcctct ttgaagcgct
    13321 cttgctttcg agcggcatct cccaaaccga caagggcata gttattttct ttatcttttt
    13381 caagaatttg aacaaagcat tcaaccgctt catcgaaacg attttccttt aggagctgat
    13441 agcccttttt agagatctcc gcaacctctg tgttctctaa ttggatttca ttaggaattt
    13501 cgaaaaaatc atcattgtag ggcaaagatg gcatatcact catggctttt tacctcttgg
    13561 cggaaagtat ttttaaatat ggctccttgt ggcctaacta tttattacct aagtctttcc
    13621 gtcattcttt tactcttttt cggtttttat tatacctttt agttcatatc aatgcaatat
    13681 tttcagttgt ttcttaataa ataaaatatc atattcttcg atagaaatgc acgatagaac
    13741 ggccaaagat cctcatatca tagcatctta gtcccgagat ttcctgcggc agattgtctt
    13801 cgcgaggaaa gtggattaga accaggcttc catcatgaat agaagaagat ctatcgagtc
    13861 gttctaacag ttggagtttg aatctatagt cgaatggtgg atcgagaaag atgatgtcga
    13921 atgcttgttt attgcgcata ataaaggtct ctgccggcat ggccttgcag aaaacagcgg
    13981 gcttagccat ggctgcgttt tgtaataata ccgggaattt tttcctatct ttttctacgc
    14041 acacaacagg actagctccg cgcgatgcag cttcgagccc aagtattccc gaacctgcga
    14101 aaagatcgag aaatgaataa cccagcaggt cgccaaggac atcgaataca gactggcgca
    14161 tcctatccat cgaaggtctt attctcaagt ctccctcagg caccttgacg attcttcccg
    14221 aatattcgcc gcctgttata cgcatcgaac ttaccaatct tttactttga attctcgcct
    14281 tatttcgcga tctaaatcac gagcctttat ttcagcccgc ttgtcgtatg cttttttacc
    14341 gcggcagaga cctagttcga gcttaattaa cccgtgtttg agataaatgc tcaatggtat
    14401 cagcgtaaag cctttttctc ggactcttcg ctcaagtcgc ttgatctcct gacgatgtag
    14461 aagcaatttt ttagccctgt ccggatcgtg acggaaaatg gaagaatgag gataatctgc
    14521 aatatgcaaa ttattaagcc agacttcccg tccctttatc tccgcaaagg catcgctgaa
    14581 actcaccctt ccttctttga ttgactttac ctcggaacca tagagcgcga taccgcactc
    14641 aagtctctct tcaatttcgt aatcaaagcg cgcgcggcga ttaatggcta ttatctttat
    14701 ggaatcttgc gttttcattt tcttatcaga gcaggcctaa accctagaaa ttcagaagaa
    14761 tgatccgcag gcatagggcc acgtgattca atagagattt gatctgcact attagcccat
    14821 gagcctcctt ttactgtctt tgaattccct gcattggcta gcattttgaa tgcaggcgag
    14881 tcttccgtta tccatacata aggcctaaag ctatcagaag tccattccca aacatttccg
    14941 aagagatctg agaagccaga tgcatcgcgg ccgtatgaag caaccgaaag cggcccattt
    15001 gaagaacgat tgttgaaaac cccaaggttc tttatgtttc ggtaagcttg gcttgcggcc
    15061 atttcccaca tagcttctgt tggaagcctt acttcataac cacctggtgc atatccgcaa
    15121 agccattcac aataggcatt agcagcatac caggagacac cggtgattgg cttatcgctt
    15181 gggctcgata tatcgatgtc tgcaaggtag tagttgtcga caagtccttt ttcaatcaga
    15241 gttgcgcggt tttcgggcgc ccattcagga ttctccttca agaaccttgc atattgtcgc
    15301 tgtgtcactt ctgtgcttgc caggccaaat ttgggaacaa gagcaatata tggtattccg
    15361 gtgccaccgg gtgtgctggt aagccctttc agatatcctt cattgaaaat gataaaagaa
    15421 tgagagccga tgatctgtac gccgacggga ttatacagag aagtctcaaa aagcgaagtt
    15481 tctttttcta atgccgatag agcatttcta atggcatcat ccgttctttc aggggcaatt
    15541 tccgaaaatg ctcctacata gctctttgag tttgcaagca acgctatagc actgcgggcc
    15601 atagaaataa atcccaaagg acttgctccg ggcgcaccaa gcgaaaagct ggcatatatc
    15661 agatcgcgga ttgaagaggt attctgagct atgctcaagc ctgccgcaag taaaggctct
    15721 gctattggca ctgtatccgc tactggaaaa gcagcgagat ccttcgaagc ttcggacaga
    15781 accatcggca actgataaat agcgctaggt ctaccggaaa gggaccaaga tgcaaattcc
    15841 ttaaaagatg ggattagtat ggattccagc gattccgatt tcaattcata ttgtattgtg
    15901 actctggggg gcagtataag agtggcaaat atacggttcg gtaccttaac agcgatagtc
    15961 ttaggctcga agccacgctt ccggatttcg atttggtatg agcctggcct aagccttatc
    16021 ccatcagatg tatttccaat atatgtgttt tcagcgtaaa gggctgctac atctggatta
    16081 ccttcgaagc ttatcttggc gccagggtta acaagtcctg ggttgactaa aaggaaaaag
    16141 agaatgataa agatcagcac cgcaaagaga attggtatgt atatacgtgg ccgaatgcca
    16201 aagatcggct ttaaggttac tttagccttt tcaatatctt gatcggtgat tttttcgctc
    16261 tctttcatta taaaagcagc ctagccgatg ggcaccgtct tgtcaacgat atatgctcat
    16321 gatagcttga aatcgggatg aagaatatgg acattctcga taagctcgtt gtgttttctg
    16381 aagacctgct cacaagaagg aaaaagagat ctctccgcgc taagatgaaa cagcaaaaac
    16441 gccatcccat tttggactgg atatatgcca tcctttgggc tgcttgtgtt gtgcttgtca
    16501 taaaccaata ctttttccaa aattaccgaa taccatcggg atcgatggaa aaaacgcttc
    16561 tagtaggaga tatgattttt gtagacaaac tctcctatgg tcccgaactt ttgcctggtg
    16621 tcttaaaaac cccaggagtc tccacaccta aacgaggaaa cgtcattgtc tttgaaaatc
    16681 ctacatatct ctcgcgcggc ccgatctata cgatttttca tcaattgctt tatatggtta
    16741 catttacctt ggtcgacata gacagagagc caaccggcga gccgcgagtg cactatctca
    16801 taaagagagc cataggggtt gggggagata cgcttcgcgt aaggaatggt gaagtattca
    16861 taaggcctag gggatcatcg gtgttctttg atgagcggat gttgatggat ggtcttggat
    16921 tacctgcaaa gatgcaacga ttagttgaat caagcgaata tccagagata gacaatgtcg
    16981 gcgtagcctc agcctataca gaacttggcc tttctctccc ttctcgagta gggatgccaa
    17041 atgtgcagca tgcaaataag gacgctttcc agtatgacat ggttcgtgtg accactctgc
    17101 gagatgcgaa tccctctgac taccgaaacg cacaacttgc acagcgctat gaaaatggat
    17161 ggtatattgc ggattcgcgg ctctttccta tgggtgacaa tagagacaat tcacgcgatg
    17221 cacggtattt tggaccagtc gctgaaaaaa aagtgctagg ccatgctctg tttatttact
    17281 ttcccctcag tagaattggc agtatccatt gagagtgatt atttatgttc gctaaacgct
    17341 ccatacgata tgcagaaaga atacagcgga aaaggactcg aaacagaata ctcctatata
    17401 tcgctctttt tatcgttctg tgcattgtct tgaggacctt tgtgtttcag acctggaaag
    17461 ttcgcgggga gcttatgaag ccaacgctca gagatggcga catagtcata gttttacctt
    17521 gtctgtcttt ttcggataaa atgctcgtca gtttggctgg catccctcaa aaaggcgatg
    17581 tcgttcttgt tgaagacggc tccgaatcga tcgttcccac tatgacgatg atcgaggatt
    17641 cagcattgag atttatgact ctgcagaaat attcacttct ggcagccgaa tttggcaaag
    17701 gcttcggtct tccgacagta atgagaattc agagtatcca acacgataag gatatttcca
    17761 agtcacagcc tgtatatagg cttgctacgg actgtcagag cgataaaaac ttcatgacag
    17821 gcgaccgaat tgttccacca tcgctaatta agggcagagt agtcttgaag atatggccat
    17881 tagctcgaat tggaaaggtg caatgaaagc atggacttca caggtttttg ggcatgagag
    17941 aggaatattt ccaattatcg atccattcaa gatacgctcg ctatatgtgc atattccatt
    18001 ctgtgcggat cgatgttctt attgtgattt tttctctgtg cctctgatca aagatccaac
    18061 tttctctaca atcgaatatg aaatctccta tgtgcaagca acgcttatga atattcggaa
    18121 ttggtcgaaa ctgtttggag cagggccttt ttcgacaata tacctgggtg ggggaacgcc
    18181 atcggttatt gatagacgag cactcaaaat actgatagac gaccttgaac aatttgtgga
    18241 tacagattgc gagtggacca tcgaagctaa tcccgatagt cttgataagg ccttgctcga
    18301 catgctcgcc ggaactggag tgaatcgtat ctcccttgga attcagactc tctcagaaga
    18361 agaatggcct attttgcgac gagtaggttc tatagatgag gcggtgcaag ccatagaact
    18421 cctttgcgag tatccatttg agttatcagt ggatctattg atgggtattc ccaataagac
    18481 aaacaaaggg cggcaaggcc acagttccat tatgaactca cttgaatacc tcataggccg
    18541 agtttcacac ctttctatat atgacttaac actggagaaa gggacattga ttgaaagcca
    18601 agtctcttct ggaaaattaa ttatgcccga ttctgatgaa ttagccgaag agcgtgataa
    18661 ggtcgattta tatttagaag gccagggatt tcatcgatat gaggtttcga attatgcgaa
    18721 acccggtcat gaatgcaggc ataatgccgt atattggaat atggagccct atcttggagt
    18781 aggaagtgga ggagtgagca cgatccaata ccccatgtgt gaagaaagcc aggaattggg
    18841 cactattatc agaatcacag agacaagaga cataagcgca tatatcgaag ccccaatgga
    18901 gattcctaaa gatatagaga taatcgataa aaaaaccgct ctgttcgagt ttctaatgat
    18961 gggtttccgg acatcgcgag gaatagatct aaaaagaatt aaatctctct ttggaataga
    19021 cgttgaggag tgcatacctt ctgccctcaa gcgatggaat agcgaaatag taagaaatgg
    19081 agattttgtt gcccttagac cggacaattt tgatatactc aatcgatttc tcgttgaatg
    19141 tctagaagaa ctcgatttat gaggttttgg tatggctatt ttccagtatg cagtgatttc
    19201 atcgattttg catgacgagc ctcagaaaaa gcagttgctt agtgttttcg aggataaact
    19261 tcagagattc ggaggcgaat tacacacgct tgaatctaat atgattcatc caagaaactc
    19321 tgagctgccg cttttcatgc tcgtactgac aggtggaact gagtctgaag ccatgagctt
    19381 gatttctaga gaaagaatgc tcgatctcag aatgcccata gtgttattgg ctcatccatc
    19441 gcataactct ctgcccgctt gtctcgagat tcttgcaaag gttaaacaag agggtgggaa
    19501 agggttgatt cttcaagccg attcttcagg agcattcgac gctgaagagc tagaggacat
    19561 aattgccgtc tgtaagagca gaattacaat gcgaagctgc caaatcggtg taataggtag
    19621 accttcggac tggctcatcg cctcgagtca ggatttgcat gcagctatga actcatgggg
    19681 tgtaaggctt gtcgagattc ctttagaaga acttgtgcgg aacatcgaag agatacgctc
    19741 cagatcctgt ggagttccag taatcgagga ttttcaggaa aaggccgaat tctatgctga
    19801 atcgagcaat gatgatattc taaagtcaat acagatattg caggccttaa ggattcttgt
    19861 agataaatat agactttcct cacttactct gcgctgtttc gatctcattc tcaaagatcg
    19921 gagcacaggt tgtcttgctc tgtcccagct tgctgatgag gggatcgatg caggttgcga
    19981 aggcgatatt ccatcgatca taggcttaag ctggatacgt tctttgacag gacagattgg
    20041 atggatggcc aatccatctc atatcattgc aaagaaagga gaagactcag ccgaaatgct
    20101 gatagcgcac tgcacggcgc ctcgctcaat tctatccaga tatgcgctgc gcagccattt
    20161 cgaatcaggg cttggggttg caatatcagg aatcctgccc aaggaagaag tgacactttt
    20221 cagaataggg gggaagtatc ttgataaact ctggtattct ccggctcgaa tcatagcaag
    20281 tccgcgcgac gaaggcttat gcagaacgca agctttgata aatctcccta atgaaaaagc
    20341 tcaagagctg cttataaatc cgctggggaa tcacattgtc atggttagag gcaattggac
    20401 cagaaggatt tcgatctctg cacaacttta ggctttcggc tattacctat acaaaaaagt
    20461 catagtttgg tcgaatgcgc tcggcgaagc cctcctggaa agatatatta gcgctttttt
    20521 ccgtaccaat ggtgcttggc ggaccatgac cgggaaaaat aagggtgctt tccggaagac
    20581 aaaatagttt ttgcttaatg cgggcaataa gcgcattggt attgaaaaca ctatttgttt
    20641 ttccaataac gcccgcatgg agcacatcgc cggtaaaaag aaaataccct tccatgagaa
    20701 atacaattga atcctgagaa tggccgggca ccgctattgc ggttatatca aagcctgctt
    20761 cgctaaatgc atctccgtcc ctcagtttcc tgcactgaaa tccaagcaat tttgcatcag
    20821 aagcataaat agtcggacta tagatcttca tgatagtaga taatccattc acatgatgaa
    20881 tatggtcatg ggtgacgagt atgccgcaga gatcgagctc gttttgctcg attctttcga
    20941 tcatagtcgg agtgatctct gaaggatcta ctattatagc cttctttaaa gattcattta
    21001 caattgtgta tatattcgaa aagccataga ccgaataatg atggtaaatc ttcatagata
    21061 gagatgctcc agatctcgga cagcctcagc aatattcgat cttgagaagc tgacattttg
    21121 ctctttagaa ggaatataaa aggctctctt gaaatcgcaa ttgtcaatat tgacagtatt
    21181 catgtcgcac attatgaatc gagagttata caaattcgaa cccgcaaatg tacaatccac
    21241 tatgacagag ccattgaagc atgattggat gagttcggaa ttttcaaagc tacagtccat
    21301 aaaagaacat ttcccaaagg aacaaaaatc tacatcggcg ccagaaaggt cacaagagaa
    21361 gaagctagaa ctatcaaaga aacataaatg gaatgaagct cctgtaaaca gtgaatgctg
    21421 aatccttgcg tttctaaaag agcaaccaac gaaacgatag cgagaaaaat cgagacccat
    21481 aagaaatgca ttatcgaaga ccctattaag aagaatgttt ttgctttgca gctcctgcat
    21541 ggccttcttc atgcgctcag gatagtcctg caagtgataa tagcacaggt tttctagtga
    21601 gaaagcaggc aagctacagt cttctattga gcaagcagaa accttgaaca tgattatagc
    21661 ctatatgatc ctgcgaggat tctcaagact aaaaaaccaa agcccaaaag tagatataag
    21721 aggctgcagc tattcctatt gatttcaagc gcttgtcagc atttcgctta caatgtagaa
    21781 tttttgatat gtgctataaa tgcggttctt atttagccta tgattatagg ccaggattca
    21841 atgatacctg tgatatatgc ggaaaagacc tgcacgcgtg cctgatgtgt cgtttttata
    21901 caccaggtgc tcattgggac tgccgagaaa caatagagga gcttgtgcaa gataaagaaa
    21961 aacgaaatca ttgtgaattt tttatggtct ctgagaaata tctcgaaaaa ggggaaaccg
    22021 cagattcaaa gcaggatgat gacgcaaaga gaagattcaa tgccctgttt ggtgaataga
    22081 tatctaagga gaaatgaacg gtgcgcatct gcgtatttga ttcgggcata gggggattgc
    22141 ctttcgtcca ggctgtgaat cgggagtttc ccttcttaga aatagattat gtagctgatg
    22201 atgcaggctt cccgtatgga acaaagtcac ctgagatgat ccgcgatata ctctttgaac
    22261 gatttaggag aattcgggca aggattgatc ccgaagtcct catactttca tgtatgacgg
    22321 ctgttcaaat aggactaaag gagcttcagg cagctcatag aacattacaa ataattggcg
    22381 ccatacctcc tatcgcagag gcagcgaatg aatcgcagac tcatcgaata gcgttacttg
    22441 ccacctctcg tactaccgag gattctttcc ttgattattt gatagctaga gatgctcctg
    22501 attgtgaagt tatcagaatt cctgctcaag atctgatcga atatgcggaa caacgtcttc
    22561 cttttgcatc taagctttcc gcacagacaa tggtagctcc ttatatcaat tatgcgctca
    22621 aggaaggaac agatcgaatt gtattggcca gcagccaatt tatccttctc gaagattcta
    22681 ttactaatca attgaatgaa ctacaagctt tcaatgtcaa atgtctggat ccgaggggga
    22741 aagtgatttg ctctctccaa aagctcattc ctgataaaga gcatagtgaa ggtcaattcg
    22801 taaagccaag attctatctc accggggacc aaggtgccaa cccttcttta attgcatggt
    22861 cagaaagatt caattgttcc aaaccacagc ttctatgaat ggcaatagta aggaatccac
    22921 ggttttagga gttgttgtct cgtttagcaa tcgaaatgca aaagtcagat gcagtgatgg
    22981 gaatattttg acatgctccc tcaaaggcaa aataaccgat tcatcattcc aaggctacaa
    23041 aagtcctgtt gctggcgacc atgtatcggt aatccccata tcgaaacaga atggagtcat
    23101 agaatccatt tttgagagga aaagtgaatt aagtcgctat aacgaaaaag gggaaacaaa
    23161 acaggtttta gcagctaata tcgaccttgt tgtatgcata gccagtacaa gagcacctcc
    23221 ttttagagct cgatttatcg atagggtggg tgtgatggct gaatattgtg gtctgccttt
    23281 tctaatagtt tgcaacaaga tcgacttagg tctggatgtt tctatcgaat ctcgtctcgc
    23341 tgcattccag aaactcggct atggagtggt caagagcagt gttcgggatt ctataggaat
    23401 cgatgaactt cggacgagcc tccttggaaa aacttcggtc tttatagggc aatccggtac
    23461 tggaaaatcg agcatcataa attgtctaat tccgggagca gatcagcgaa tagccgagat
    23521 ctcggacaaa cataagagag gaaagcacac tacaaccctc tcagccatgg tttcttcgtc
    23581 aaacggcgat attgacataa tcgatactcc gggtattcgc aggcttgcga tacgaaatat
    23641 cgaaccggat gatcttggat tttattttcc tgatatggtt ccatttctgg ggctctgtga
    23701 gtttggtgct tcctgtacac atagatatga gttgcactgc tttgtgaaac aagccgtaca
    23761 agaagggatc atacatcatg accgttatga gagctatctc agaatgcgtg cggaattaga
    23821 agaaacaaga aatacaaaga caaacgagtc caggaagcca ctcaggtctg ctacatataa
    23881 attcgaggat gaagaatggt gactaatcag catagccttg cgctcctaga cttttatcga
    23941 ctgcgtgatc gcttggaaga atactgtttt tctcacgaag gacgggcgct cttgaatagt
    24001 accttgccct gttctcagga aaattcagta aagagactta agaaggactt gtcgctgctt
    24061 gttgaaggac tcaacgaaaa agagattcct tcgtttccct ttccaaacat cgagaatccc
    24121 ataataaaag cagccaaaga gggtacggtt ctcgaagctg aggagctttt cgctattggg
    24181 ctctgggtaa aatcttatca tcagcttttt tcttttctgc ggcgaattct tgactctttt
    24241 attgaagaat caaaagaagt tagcgcagaa aactccatgg atggcgagat ccggcaagaa
    24301 gattccataa atgacgaatc ttgggcagag tggggaattg agaatattgt gtattcggcc
    24361 cctgccttga gccaagttac acagatgata tttgcagtca ttaccgaaga tggtgaaata
    24421 aaggatttgc cccggctaaa ggctctaaat ctggcaattc aaagaaccca ggccgaaaat
    24481 ttacacataa tcaatcgata tctctctgat ccttcgcttc aagatgcttt gcaatcgaac
    24541 atagctaccg aacgcgataa tcgagtcgta atagcaatca aagctaattt caagggaaga
    24601 ataaagggaa tcgtacacga attttcttcg acaggtcaaa caatctttat agagcctatt
    24661 gaacttgttg aacgaaataa taagcttctt gagcttgagt ctaaactcaa tgaagagata
    24721 agggctattc ttagagaaac aaccgctgca ctcaggcatt ttttgcctat cctaatgcga
    24781 gcaagaggat tcttggctgc cgcggatgct cgctttgcgc gggctattca ggtcaagaga
    24841 gagggtcttg ctatagcagg tcatcttgaa catggtatac atctttatca agccaaacac
    24901 cccttacttg ggaaaaaggt cgttcccata gatgtggata ttccagatga ttcaagaatt
    24961 ctcataatca ctggtcctaa cacaggagga aagacagtct ctctaaaaac gattggcctt
    25021 cttgcgcttt tgaatcagta tggtgccggc ataccagcat tatcaacttc agcatttgca
    25081 gtttttgata caattctcgc agatataggc gacgaacaat ccatcgacca gtcattatct
    25141 acattttcag gacatatgaa agttatttct gagataatat cttcagctac cgatcgtagc
    25201 cttgtattgc tggatgaatt gggagcggga acggatcctg aagaaggttg tgcaatagca
    25261 atggctctgc tggattattt ataccgattg caatgtttcg caatagtcag cacccatcat
    25321 ggagtcctta agaattatgg atatactaaa cctggctgcc tgaatgcctc tatggaattc
    25381 gatgcagaga gtcttagccc gacgtacaag atgataatgg gcgttccggg agaaagcagg
    25441 gctctcgaaa ttgctggcca gattggcttg ccaatggaaa ttgtcgatat agccaagagc
    25501 tatctcgaag atgagcgatc agattattct atgattattc attctttggg agaaaaacag
    25561 agagagctag agaagcttga actggaaaag aaaaagcaac tagggaaagt acttgagtta
    25621 cgccgcaagg tcgaccttga gaatctcaga attcggcgaa gagaactgga actcagaaga
    25681 cagggaattg ctgagctcaa acattttatt aatgaatcta gaaggatttt cgaaaatatt
    25741 gtgcgagagt tgagagaaaa tggcaagaaa gatggggata catccgcagg acgagaattc
    25801 ttgaaagaaa tctcagacga aattcatcga caaagcgaga cgcttgagag ttttgcacaa
    25861 gaaaccaata gaattgaacg ctcgctaaaa cctgaaatgc aaggcaatat aggaatagac
    25921 atcgaaaaaa tgaaattcgg agataccgta atattccaca atagagaagc tgttttcctt
    25981 cgaaagcttg ataaaaagaa tgcgcttgta caaataggtt ctcttcgagt cccaattgaa
    26041 gtcgaatctc ttagtcttcc caaaacgatg gatagattcg ataaagccgc aaagcctggc
    26101 tatcagattg aactcgcttc tgattccatg ggaaggaatg cgccagtagc cgaactcgat
    26161 gtacgtggca tgagactttc agaggctcta gatttgttag agcaccagat cgatgctgca
    26221 agtctcgctg gtgttagcaa tttttccata attcatggaa ccggagaagg tattctctct
    26281 aggggtattc atgagtggtt gaagaagcag atctcagttg cagattacta ttttgccagg
    26341 ccagaagatg gaggttttgg gaaaacctgg atccatctaa aagaataaga tcaagttcgc
    26401 tggtctcaat gaaatttcac tgtttagttt ctggaaattg acatagcctg tgttttgcta
    26461 cctttttttg tcaattgaca ccaatttgtg caacatgata tcgttatttg gtttctcaaa
    26521 gccaaaggag ttttcatgtc tggacatagc aaatgggcga gtattaagca taagaaggga
    26581 gcacttgatg ctaagagggg gcagcttttt acaaagctga tcaaggagat ttcgattgcg
    26641 gctcgcatgg gtggaggcga tatcgaaggt aatccacgcc ttcgtacagc agtactaaaa
    26701 gctagggctg cgaacatgcc taaagacaat atcgatcgag ccataaaaaa gggtaccggc
    26761 gaacttgaag gttctatcta tgaagagcta ttctacgaag cttataccca gggcggcggg
    26821 gcaatgctaa tcgaagtttt gaccgataat aagaatcgag cagctgcaga aatacgaaat
    26881 ataatcacga gatcgggtgc caatttagga actaccggat cggtcgcata cctttttaag
    26941 cgcaagggca tcctgactta tgatagcgaa aagtataccg aggatcagat aatggaagct
    27001 gccctcgagg caggagcaga tgatgtggtc agcgaggatg ggaccatttc agtatatact
    27061 gatccctcat cttttgagga tgtattaaat gttatgaacg acaaaggctt cgaaacactg
    27121 ggagcagaaa tatcaatgct gcccgactct tatataactg tcgatctgga gacagcccag
    27181 aaaatacaaa agctcatcga caaactggaa gaaaatgaag atgtacaaag cgtctatcac
    27241 aacatcgaga tgcccgagct cgaggaatga tcggtgcttt gaattctcga agttctctag
    27301 tagctattgg aatcgatcct ggcatagcag ctgttgggta tggccttgtc agaactctgg
    27361 aaggaagact ctctctggta acttatggtt gtattgaaac ggaatcggac gaacctattg
    27421 gtaagagact gacttatata tttgaagaga ttaggaaact tcttggacaa tacgctcccg
    27481 atattggtgg tatcgaggag ctttattttt ttagaaatgt ctcctcggct tttcccgtgg
    27541 cagaagctcg aggcgttatt agacttgctt tcgaaaagac aggcatagcc ctaaaggaat
    27601 atagtccaaa tgctatcaaa aaagcagtga caggctcttc gagagctagt aaaagccagg
    27661 ttcaggagat ggtcaagatc ctcttaggta tgtctcagat acctaccccc gatcatgctt
    27721 cggatgctct tgccgcggct atttgtacat tgcattccat tggtcctgca ggctatactc
    27781 tctaacatgt ttaacagaat aaagggtgta tttagcggcc acgaggatga agcccttcac
    27841 atatcaaccg gtgccctgga atgggaaata ttagttccaa tccgggatcc gtccttttac
    27901 aatcacacag gagaagaaat agagcttttt acatggttac atcactacga agacgcaatg
    27961 cgcttatatg gttttttttc ggcattggaa cgcaagctat ttctagatct gcaaaaagtt
    28021 gaagggattg gtcctcgcca ggccttcaag attttgcagg gtatagcgcc gcgctcccta
    28081 gtcaccatgt tagatagtgg tgatctagct tctctgcaaa aaataccggg catcggtcca
    28141 aagacagccc aaaaaatgat gctcgcacta aaaggaaagt tgatcactca agaggaggct
    28201 tctgccgtta tttccggttc ccaaggacgc tttagggatg tggttcgcgc tctggttgag
    28261 atgggctttg aacgaaaaga agtcgagcgc gtaacagctc agctggcttc atctttagaa
    28321 gatagtcctg aagcggaaaa agaattgttc aaaagggcgc ttcttgagct gtccgctgga
    28381 gctggagcat gagtccttca acaatagata acgcatcttc atgcagtcca atgaatcccc
    28441 tctataatcc ggctgaagat gacaaggaag cctctttgcg gccgcgttac ctcgctgaat
    28501 ttcagggtca acagaggatt aaagacaatc tgctcatttt tatcaaggct gcacgtgata
    28561 gaggtgaagc cctcgatcat ttatttttaa tgggacctcc cggccttggc aagacgactc
    28621 tcgctcttgt agtagcatcg gaaatgggtg ttgatttcaa acctacaagc gcgcctgcta
    28681 ttgagaagcc aaaagatctg gctggcattt tgactagtct tagacctggc ggtgttctgt
    28741 tcatcgatga aatccatagg ctcaagccat caattgagga gatgctctac atagctatgg
    28801 aagattttga gctcgactgg attatcggac aaggccctgc ggctcgcaca gtcaggattc
    28861 ctattccaca attcactctt gtaggagcga cgaccaaggc aggcatggta tcggctccac
    28921 tttcaagccg ttttggaata tctctgcgtt ttgactatta ttctcctcaa gaattggcaa
    28981 gtgttatccg ccgttcttcg aatattctcg atataaaaat caatgaagat gctattgaac
    29041 tgctttcagg aactagtaga gggacaccgc gcatcgccaa cagattgctg cggcgcatgc
    29101 gggattttgc ccaggtacga ggggaaggta ttatcacgag agatatcact ctagatgggc
    29161 ttgaaagact cgaggtggat tatcttggcc tcgaacgtct tgatcgagaa attcttaaga
    29221 caataatctt gaagtatgga ggcggtcctg taggtgctga aactctcgct gtctctgtta
    29281 gcgagagcat tgagagcctt gaagattatt acgaacccta tttaattcag ataggtatgc
    29341 tagcccgcac gcccagagga agagttgcaa ctcaaacagc ttaccggcat ctcggaatag
    29401 aaagacctaa tgaaaacgga cagtttcgat tttgatctgc cggagcattt aatcgcacaa
    29461 tatcccacgg agcagcgcgg tagatcgcgc cttttggttt tggatcgcaa aaccggaaac
    29521 accattgatt ccatggtgga agacatagtt tcctttattg aaccaggtac tctgatggtg
    29581 tttaacgact cgaaagtgcg aaaagccaga ctatacggaa catgtgaaca gacagggaca
    29641 attgtagagt ttgtctttct ctccccatgg aaacaggatg acccaacaag ccttggcgac
    29701 tcatcatccg atcatgaaag ccaaggcgac cttgtaaaag cttgtaagcc gatatcaacc
    29761 gtctcgcgac tatggcgagc catatgcaat aaatcgaaac agcagaagat tggccgttcg
    29821 tatatgtttc cagggcagct gagaggttca attatcgagg ataggggaca tgaaaaaaca
    29881 attgaatttt cttttatcgt gggcgatgaa tattttgatc tctatggaca tgttcctctg
    29941 cctccttaca taaaacgaga agatacaagc gatgatgaac tgcgatatca gactgtctat
    30001 gcaaaacata ctggttctgc cgcaagccct acggctggat tacattttac gcctcaacta
    30061 cttgacgagc tttctcgtaa agggatcgaa atcgaatacc ttaccctcca tatcggattg
    30121 ggcacttttc aacccgtaag agctgataat gtcgaagatc accaaatgca tgaagaatgg
    30181 ttcgatatct cgctcgaagc tgcagataaa ataaatctag ctttaagcga aggaagaaaa
    30241 gtactagcag taggaaccac ttctatgcgg gctttggaat cagcctggta tggtggaaaa
    30301 gtcaagcatg ggaatttttc taccaggatt tttatctatc caggttatga attccatgtg
    30361 gtcgatagcc tttttaccaa ttttcatact ccaaaatcta ccctgctcat gttggtgtct
    30421 gcttttgcag gaagagaaaa gatcttggag tgctatcgtc tggccataga aaaaaattac
    30481 cgctttttca gctatggtga tgctatgcta attctataat cctttttcgg aaataatgaa
    30541 aatatgccaa atctattatt caaggcaatt gagaactaga ttcagcactc aaagccaaga
    30601 aaggcaaaaa cgatagattc tatttgccgc tgtaccgatt cgatatcttt actaccatcg
    30661 atgcgctcta tgcgccaacc tttattggaa agacgataag ccatactctc atacattgtc
    30721 tgcactcttc tttggaattc gagcgtctca tagatttctt tctttaatcg agaacgaatg
    30781 cgagagatac agatttccgg atctacttca aataggagcg tcaagccagg ggctggaaaa
    30841 gcgctattca ataaccaagg caatctatgt ccgcatgttg ttccttgata agcgagacta
    30901 gatagaacat atctatctga gataaccagt gaaccgctct tgagactttc catgatgcca
    30961 tttttaccat atagatgctc atttcgatcc gcagcgaaca gatgcgcaac tgtggtggga
    31021 acaaccatat cttcaccaga gaggatggtg cgtatcagtt ggccaatatg attcatggtg
    31081 ggttctgccg taaccataca ggaaaatcct ctagcaatga gggtttctcc cagtaagcga
    31141 acctgggtcg ttgttccggc gccatcgatt ccttcgatga caatgaaacg atttttgagc
    31201 tcgtctgtgt tatatgtagg catattatgc tagtatatag gcgactttag attttgtcat
    31261 tcagggttcg cattgaatga ggacgaaaaa acccgtaata gtaatcgtca tattttctct
    31321 tgcactcgcc atctggggca gcatcgtgat tgccgatcta gtgcagcgcg aagtgcagat
    31381 gctggccatt aaggctttgg ataggctaca gtcggcagtt gtcgatgcca ctggcctaat
    31441 cctgaaatat gacaatttag acatagagtc tctaaaccga ttttccatgc atggcattgc
    31501 gcttattcgt tcagatgctg cagaacataa tggtaacaag ccttcatctt attcggagtt
    31561 ctatccttcc tcagaaagat caaattatga agaacaaccg ccgctgcgaa tcaaaaaact
    31621 atcaatcagg ataagcattt gggctttatt ttcaggtcat aacaatgaag ccgtgaggga
    31681 tatcacggta gatgatgtcc agatttctct ttatttgccc aatgatgaat acatcataaa
    31741 aaagatactg gagaatttcg tttcaggaaa agcagaaagc gttccacatt ttgaactctc
    31801 aattgggcca gtgcgcgctg aagtgcgaga gattgcaaag agctccaacg cttctatgga
    31861 taataatgtg attaatgaag gaagcctgac catagagtct ctccagtttt cgagcctaac
    31921 tgattctcct gtaatcatag cgccttcagt gattttgaag actagtggtc ttcttggttt
    31981 accgaaggac ttgaatgcag aatttggcct tagaggaagt ggaaatgcca atttatccga
    32041 tatcgatatg aacatagatc ttgaggcaag ttctacgggc tggcacatta tgcctcaaaa
    32101 acttctgctg gagaagcgag gtaagaggat agagtgtaag aagttaggag ggggtataga
    32161 attatcgggt agttatgagg ggggggtatg gggaatagag ggtgtatttg aggggtacgg
    32221 tgttggggaa gacatagagt taggggggga gtggggggaa atagttaagg ggagtgaggt
    32281 aagtggcgag ataggggtag gtggtgattt agagggatta agtagatatg aagtagattt
    32341 aggtattgat ataggggtag gcataagtat aggaggatat gatttagggg cattaggggt
    32401 aagtttaatt ggagtagggg atgcggttag ctatataggt ggtattaggg tagaggtagg
    32461 tgggaatgag tttaggtatg agggtgaggt aggctatgag gggataaggc taggaggtaa
    32521 ttatgagctt agtggagtgg atatagggat agggggttat ataagaggag ggatagggga
    32581 atatgaggta ggagtaggta aaggagagct taagggtgtg gagatagggt taggggtatg
    32641 tagggttagg gtaggggtag aggggtatga ggtgaggtat gagggggcag tagggggagg
    32701 ggaagtgaag gtaggaggta gtataggcaa ggctgggggt tatgaggggg tagtggagtt
    32761 agaaggggta ggaatatcag aggtgatggg tgcgctaggg gtaggaggga tagagggtga
    32821 ggtgagtggt cgagtatatg gtttagagag gggaggtaag aggtcttgga gtataagtgg
    32881 gttagcatat gatggagagg tgatgggtgc agaggttagt ttaagagggg atggggtagg
    32941 ggatgaggca gggtatgaga taaaggtgtt agaggcgagt ataggagatt atgatatagc
    33001 tttgaggggt aagggtagct atgttgggga attatttagt ggggaattca gtgctggggg
    33061 tatgagttat gaggtgagtg tagaggcaag agggggtaag gtaagggtag gcatagggga
    33121 gggttttaga ggggcatttg agctagtggg agggcggtta gagggggagg tagggatgga
    33181 taaatttggg ttagggttag gtgaggggat gataagggta ggagggttag caagtgggtg
    33241 gtatgaggct gggggatggg gaataagttt agaggggatg agtttaagct atgaggggga
    33301 gggtaagtat ccggaagtaa aggttagtgg gttgataggg gatagtggta tacgatttga
    33361 gtggctagag gtgaagggtg atgggtatgg gtttaagggg tcaatagatc tgaagggtga
    33421 gggtggtgag tatatattaa ttgggagtgg caagggagag ggggtattag gggaaggatc
    33481 tgagtactca gtatatgggg taataagagg taagggagta gatttgaggg taggagtaca
    33541 aggattaggg gtgggtagtt atgtggtaga aggttatgct gatttgagag gggaggtgga
    33601 cataagggag gtattgaagg gttctttgga aggtataggg gacataagag gtgaagtagc
    33661 ggtaggtttg agtggttctg ggttaagggt agatgagcaa cgatttgagg tggagaagcg
    33721 aggtaagagg atagagtgta agaagttagg agggggtata gaattnnnnn nnnnnnnnnn
    33781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33841 nnnnnnnnnn nnnnnnnnnn nnnnngctat gagggggagg gtaagtatcc ggaagtaaag
    33901 gttagtgggt tgatagggga gcaaggagca gatctcgatg tagattattt acaatatgca
    33961 ggacaatcgc taaagggctc aatgattatt caatattcct cgatagctaa tctattaaag
    34021 gatacaaaga tagattattc attcttggac attaagactg atagaacaat tctggaagga
    34081 gggattgtag ccttagatga taaactagaa gtcgcgatta agacatcatc gattccattg
    34141 gaagaagtat ttccctctac tttcaatatt tctggagata tagaactaaa gggaagttca
    34201 accatttcaa tattgagtaa aaagctagat ctgtctgccc tgtcttctct aaacttgcag
    34261 ttcaacacga gaaaatcaga aatgaaagga atccctttct cagcagcagg tacaataaat
    34321 ttcaaagatt gcactttttc tttagaatct ggaatcttct cttatcagaa ctacagattt
    34381 gaaaatatca atgctgaata caatctagaa tcaaaacaag tcgcttatgc gctaaattcc
    34441 aaaatagtga ttggagaaca attgctgagc gccaacctga agggcttcgg agacaacata
    34501 gatagctcta ttttcaagct cggtgctgcc caagccttcc atattaccgg agagataaat
    34561 gatgccagct taggatctaa gaccatagat ccacttagct attctatttc caatgcagat
    34621 gataagcttg atattcatct aagccaagca aatggggata gtatcgatgc atccataact
    34681 aatatgacag actttgatct tgttttaaat gatctattca aaataaaagg tagagccaat
    34741 ggcaaaatcc aagccaagga agtatgggcc gatattgctt tggagaaaat tgatctaaaa
    34801 acgttaggag ccttgatctc aaaagatgat ctaggagata cttcaggcat tgcttcagcc
    34861 gaactaaatc tctccggaga tatatcagac cccagaattg agggcaagat tctattcagc
    34921 aatctgatta tcgatcaaaa tctttatatc gtagaaaaaa tcgggccatt cgatgcagaa
    34981 attgctataa atgatggggt agcggaatta agtcctacaa ttatcaacat tggaacaggc
    35041 gatatctctc ttgctgcgac tgcgaacttg gctagatggc aactcagcga tatcacggtt
    35101 ttagtctcga ctctcgagaa agcttcaatg cgcttcaaag gtaaaatcgg tggattgaat
    35161 actgatggca tatggattaa aggagatata aaagcggcaa tatcctcgaa ttccatcgaa
    35221 attggcggcg acatgcttct tgatgacggg acactagaag tcgatcctaa tggttttatt
    35281 tcctccgaaa atgcgcaaac tccagtctca cctctctatc tcaaattaat ggttacattt
    35341 ggaaagaatg tccaaatgta tatacctggc aaggatatat cccttatcag aggaacaata
    35401 tctcctgctt cgacttttac ggtccagtac gatcaggcga gaggcgattt tgcggtaaat
    35461 ggtaggattg agctgcgctc cggatacgta tattatcttc tcagagattt ctttataaaa
    35521 aaatgcacaa tagaattagc ggaaagtcag tccaaattta atccagtcat ggatgtcatt
    35581 gctgagttga tggaacctac caaggatgga atgatcaaga taattctttc agctgacaaa
    35641 tctcccttag ataacttcag tcccgtactt tcttcggtgc ctccaaaaag ccaaatcgag
    35701 cttcttgctc ttcttggtgg tgga
//
LOCUS       DAOOWY010000010        31733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1441_length_31733_cov_9.903687, whole genome shotgun sequence.
ACCESSION   DAOOWY010000010 DAOOWY010000000
VERSION     DAOOWY010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 31733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31733
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1441_length_31733_cov_9.903687"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK105_01690"
     CDS             <1..314
                     /locus_tag="PK105_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60448.1"
                     /translation="SIGAAFFGESMFSLESNASKIGFLSLARTIFDRGFEFIDCQVYT
                     PYLELMGGIDVPRSIYLSMLDAALKKPTIRGDWSKMFPSFPERELIMYTDKNHKVMVD
                     S"
     gene            382..2196
                     /locus_tag="PK105_01695"
     CDS             382..2196
                     /locus_tag="PK105_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU60449.1"
                     /translation="MKLLFVQLPAQMPDWNSVPANVPLAAGYLASYAESKGLLNRREW
                     TILEPPIANYGSDASIIASIVVREPDVVAFTLYSWNIERSLFLAEKLSSQLPRARLIA
                     GGPEVVKPMPITSKSPFNSLICGEGEEAFADILQDILEHRPISSFYSSDKLIDLNKLP
                     NPYLNGALLFDPNFQVHIETMRGCNSKCSYCYYGKNYKTVRYFPHEQILEVIRLASEA
                     GIPEIYIMDPNFQIRPDFAGKLRDIAFVNHAHTSIHTELRLEDIDEEIAGLLKEAGIV
                     SVEAGLQSTNSKALEAIHRSFDRKAFERGAELLQRQKIIIKTGLILGLPYDGYEQVIE
                     TFDFLGMQGLGQEAELYPLSLLPGTEIRERADEYGMSAMDPPPYLVTSTHWISYDDMV
                     DAISAFEESFDVEWAMPPAPHFQLFKEGFVSFIDLRKPENIDWMRLNPEKLSNSITLL
                     VDADDPEILSRVVRAARDLRKDNPYTLYQIVLTSETRIPSEKLVERIRDAFMNSAHYY
                     ELMNSFSPDPQTSYQTRIFFATKNFTLAYRALEEAQDMETMVILSGKGGYNSDRLAEL
                     LPYVIFDKLSLPFDRLYELISIYADFPHMLIEAPEGLF"
     gene            complement(2239..3216)
                     /locus_tag="PK105_01700"
     CDS             complement(2239..3216)
                     /locus_tag="PK105_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOU60450.1"
                     /translation="MKIGSFMPIQSIWRTIIEQLREEFPDHTWLEGLTSDSSEIGSLD
                     LIIAGRIPADVFKAATNLKALFQPFTGINHLPSQLMLERNIQVYNVHSNAFDVAEKAL
                     ALTLAFYGRVIEYHNDLKNEIWHGFWVNRGAEDNWDTLYGRNCSILGTGAIGQELAGL
                     LKAFHCTVYGWRRTKDAKKPAHFDSIVPTLQEAIDFAEIIFIALPATKETENLISKEM
                     LMNMKGKFLVNVGRGSIVNEEGLYEALKNGVLKGAAIDTWYTYPATGVVGAPSRYPIH
                     KLSNVVLSPHVGGSTNRASAKSIEDTVANIRSYLRTGRGIWQADLSKMY"
     gene            3376..6177
                     /locus_tag="PK105_01705"
     CDS             3376..6177
                     /locus_tag="PK105_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOU60451.1"
                     /translation="MQRRSFKVGRFFSAIIAVLFLLGGCATSPAKNKEVTLNDLVIQG
                     DFEGIRKFYSNQEELNKPSNKEPYVGLYPLHIAVTQGNAQIAEILIVLGAKPDNQDLS
                     GKTALRYAVDRKQTAMVKMLIDRGADPFITDVGGTSPALVALHSDISLLDAMFNSRNI
                     NNTGPDGRTILHHASDALMPKEVDLLLSKGALVQIKDKADRTALDLALLYPEKLEAAQ
                     IAEKLILKGANPSFPEFSWFADTVRRPDYGAARFANGNTPLHEAISRYQHGFALFLLL
                     NNVDPNLKNLNGDASLHLAVKMGYVEGARLLLSHNANPDILDANQSTPLHLTVPATQR
                     LEMVKLLLAFKANPALMDIQGNTPLHKAVAQLYAPEIVETLIQAGAPVNAQNSKGDTP
                     LIICARTEHYEYARPLIEANAEIFKMNQAGENALKIALSKGYTAVDNIVLPSNVNQSD
                     DNSNSVLMTAVSLKASPDVLKLILSKGADPNAKNKAQDTALHISVRQNYAQQGIVLLD
                     ANADIFQYNAVQENPLFLALTAKPAPLEWFFRPNVITATDANGDSVVHHAARKNLPEG
                     ISYLVKKGANIEALNNAGEIPLHTAAKYDATDAIRYLVSAGSSLNAANKNGDIPLQSA
                     VLNGAFKATQMLLAAGSPVNNQNYSGETALHQASKNNSAMIIQLLTAQGAQTEIQDSR
                     GFTPLITAAANDRYDAASELIKAKAVIDTRDYLGSTPLYHAVSGEYAALIRLFLVNDA
                     DIHALNAQELSPFKLSLSKNPQITPEFYTDKLINKPDSSGDCVLHILVKSGQGQEALT
                     QALKHGANLNARNSKGDTPLMLALKKGDLNAAVVLIQAGSSLFVSNADNKIPLSAIFG
                     MDSGARMKLFSATGANSADYIGESILHYAVRANNADVVSELLSLGADRNAHNRKGETP
                     KDIAQAKGYQEILAILERK"
     gene            complement(6319..7305)
                     /locus_tag="PK105_01710"
     CDS             complement(6319..7305)
                     /locus_tag="PK105_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU60452.1"
                     /translation="MGDYLDCDKASISKLPSRKGSQEKAEQESDPLSLYLRQIARFPL
                     LDAKQEQEIGVKLEELGLLLKNYDKEITKDPQNIPLLEMRRNTLEKIRASKEVLITSN
                     LRLVVSIAKGYRMRGVSLLDLIDEGNIGLIEAVNRFDYKKGYRFSTYASWWIRQAIIK
                     CLVDQSRMIRLPIHMLNTIRQCYASAKGLVQELGREPNSIEIAERSGIALSKVESALL
                     LALGTSSLDLCTKENQNTSPVNNLKDEGSSDPYSDAFLVTLRELLSYVMDSLSEREQA
                     VLELRYGLNGEAPKTLEATGLALGITRERVRQIQERALDKMRNRRELSDSIQ"
     gene            complement(7416..8438)
                     /locus_tag="PK105_01715"
     CDS             complement(7416..8438)
                     /locus_tag="PK105_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU60453.1"
                     /translation="MVRRILKASIFFLLFGLLLTISGAEQEYHVIQAGETLYSIAKSY
                     SVPYETLASINSITDPSKIRPGVVLLIPRVHIVAKGETYYGLSKQYGISIQELKSANN
                     LSDIYVLKVGDVLIIPEKGVISVQTVQDSPPLDSVVDSVAMSQPVASASTQQNSSSPA
                     NTAIVPSNSVSTTSSTSQAVLPPNSPTTSSSAASQSTNPPAQTSAPTKADSPKTSVSG
                     KRIPDAIVNWPVDGKAQYMSGKLEGVMFQTARGASVKAVASGTVVSAGPSRGYGEVVF
                     VQSKAGFVYVYAGNESILVKTGEIIEPGKAIAKVGIDANDGNPIAYFFVFRNGQPVDP
                     SLAPRD"
     gene            8569..10230
                     /locus_tag="PK105_01720"
     CDS             8569..10230
                     /locus_tag="PK105_01720"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOU60454.1"
                     /translation="MFETSPLHAGRYSYVPKMPETFLDSIQSIKTIPTETIEPERDRE
                     VLKKFFPNSYGKPAIKFVKGEGALEDKAITVGVILSGGPAPGGHNVIAGLFDALIKAN
                     PQSRLLGFKSGPSGLIKGQYVELSSELIDQYRNTGGFDMIGTGRTKIETSEQFEQSLE
                     NAIKLGLDALVIIGGDDSNTNAALLSEYFFQKEAPITVVGVPKTIDGDLKNEWIETSF
                     GFDTATRVYSELIGNICKDASSGRKYWHFVKLMGRSASHITLECAFQTRPNVALISEE
                     VDAKKMSLSQVVEIIANTIAARSAKGENFGVVVVPEGLIEFIPEIKTLIAQINDIIAT
                     SEDEYESLPKGEAQRSYILSHLPSECISLFSSLPESIQTQLCWDRDPHGNVQVSRIET
                     EKLLAEMTSRKLQEMTAVGAYKAKFDYQTHFFGYEGRCAFPTNFDADYCYSLGYDACL
                     LILHGLTGYIAAIRNLAAAPKDWLPGGIPIVSLMNIEHRHAEEKPVIKKALVELEGKP
                     FKAFSAMRDKWALSSDYRIPGSIQYFGPPSITDTPSITLQLEQRL"
     gene            10227..10913
                     /locus_tag="PK105_01725"
     CDS             10227..10913
                     /locus_tag="PK105_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOU60455.1"
                     /translation="MKRKHITIIASIFACFLGASTLSAQVLLTADQFFSQLAEKYAMV
                     TDYQADVHITAGHQPMAGTLLFKSPSLLRIDFSQPADQVIVFDGKTLLVYLPQYRAVL
                     RQDSTDQGISVGAATLASKEGLSLLRRNYTIGWEQSPNPEPLDPGSDELVYRLMLSRK
                     SVSEGYKNIRISVSADTMLIRRLEGWTVSNDKITFDFVNIQLNRGISDNKFIYNAPAN
                     ANVYSNFLFQ"
     gene            10941..12302
                     /locus_tag="PK105_01730"
     CDS             10941..12302
                     /locus_tag="PK105_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU60456.1"
                     /translation="MGKIGDLFREARLGKGLTLEQVADETNISKHFLQGIESDDFSDF
                     PGEVYSLGFIRNYAEFLGLDASAMVEIYRSNESTRQAEVEKSESTELPLPVEDSKKEE
                     TLPQKSFISSEVEKSAKETKQTFHAKRKPKEKSIKREEPETIKAEPKASIQVDSTLEK
                     AALIDFQPKKKIGTANIRVGRVLPFVFVAIVLIIAAVSIIPRLRISPGAARVPTEYRA
                     EGLPFEQRLYPQDKVYIPLSGDVISITLESIKDKVSFNTPFGSLAVGLNEKTVVDPSA
                     DRERLVIAVSDYEADKSQNGAMVHFDVEEALSDAENSLDIIVPSTPGKAQLSQDSTQG
                     QAGGANRTQTSSTVLFRSTGGPHPFYVNVSFIAPVFFRYEADRKEWVEKYYRKGESIT
                     VNASNTITFWTANAQAVKVNVFQSAGKSVELVMGGPGEIAVQRLSWSNSQSGWAMIAT
                     QVD"
     gene            12367..13698
                     /gene="rimO"
                     /locus_tag="PK105_01735"
     CDS             12367..13698
                     /gene="rimO"
                     /locus_tag="PK105_01735"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOU60457.1"
                     /translation="MPTFFVDQHGCAKNQVDGEEISSRLINAGFLAASSPDEADAIII
                     NTCGFIEDAKKESIAAIVEAKTRLPKKKVIAIGCLSQRYPEELFNGLPEADGVLGNGN
                     LSIIPEAVTKILRGERIVLATAQNKKMPSPYYPRIVFFDYPGTAHIKITEGCSNYCSY
                     CAIPLIRGELRSRSIEDIVLEARNLIAQGMFELVLIGQDLGNYGKDLSGRCLLMDLME
                     ALSNIEADFRIRFLYIHPDHFPSEILKIIAHDSRFSPYFDLPFQHASTPILQKMNRHG
                     SATEYLNLIYRIRSELPMSMIRSTFLVGFPGETEEDFAALLNFQKTAEIDWVGVFTYS
                     LEENTPAYDMKARIPKSIARKRKAILENEQIAITANRLQRFIGVSDTFIIEEAFDHDE
                     LCFGRGWMQAPDIDGVTIINASLKPGTIAKANIVSVNGVDFNAELEYTTTP"
     gene            13695..15887
                     /locus_tag="PK105_01740"
     CDS             13695..15887
                     /locus_tag="PK105_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU60458.1"
                     /translation="MILGQIPEYLEGLNPEQLEAVCYEGNRLLILAGAGTGKTRVITT
                     KIAHLVKQSRFLPESILAVTFTNKAANEMRERAMAIEPSCERAIIRTFHSFGAWFMRR
                     NANAFDLRSEFSIYDEDDSAELVHSIFPAFTKRDCGDYAFLIAKAKDYCLSPDSPNLD
                     FISRHPEFRRIYSAYEDRLRSTGNVDFGDLILMPALLLEKDETIRNRTRQRFRVILVD
                     EYQDSNIAQFRLLQQLAGPDSMLCVVGDDDQSIYRFRGAEVKNILNFPKVFADTRIIR
                     LERNYRSHQAILDLAHSIVVHNENRLGKNLLATRPGGNKPKIAFLDDQDQEIEFCAHI
                     ARQHVKNEGEWKDIAILYRTNAQSLGFERLFPQFDIPYRIVGAVRFYEREEIKDMLAF
                     LAILANPRDEISFKRVVNKPSRGIGASSLDKIISFAFHNGLDLIEASRQSIASISRKG
                     SEGLKEFLSLIEEAKASLHRDLADQKNPGDLGSLISDFAQKSGLLEFYASKDRISSTH
                     KKENIEELVNAASDHPLSSEGLLEFLDDVMLDQSRQKNQDNADAVTLITMHNTKGLEF
                     PIVIVTGLEQGLFPRNDEEGDDIEEQRRLFYVSVTRAKNQLYLTSCRWRRIHGKLFET
                     MPSQFLSEIDPSLYKMWGDSRNKMRAVPSQYRSSSTVAVSSQSDTSTKKSEWTAGMSV
                     YHDEYGNGTVIKVTPSQSAGPLVIVQFETGKTAQFFPKYTKKLERIKN"
     gene            15917..16750
                     /locus_tag="PK105_01745"
     CDS             15917..16750
                     /locus_tag="PK105_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60459.1"
                     /translation="MRDKASEESILQPNTDSLLSHLPEINRMRGWRMYARNGSRILSL
                     WSDYGRTLLGYSPRGLVSIAKSSMDRGLFSAVPGIWNSRIEKMLHRLLPEYRNIKILG
                     SQEKAIKLLQNLEGLASSTPSIGTCKFLFDKYLKRDDELSDIISIVLPIPDFLSIGIL
                     ASKMSSSPVIEQELIEGIKGMTSIYALSKMQSEEEGVISGGLEPVWESFDRYGGVLFS
                     RSGPWLFPNYPRENHEEVFLYCLEHGLLISPDYNFPSSVPGDFDKGELGPLRSAAESF
                     L"
     gene            complement(16666..17682)
                     /locus_tag="PK105_01750"
     CDS             complement(16666..17682)
                     /locus_tag="PK105_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU60460.1"
                     /translation="MTRARNSTAMRGFIAAMISAISFSTLGIFAELLYSHGFSVLCVL
                     AWRFLVAALFLWLVVYIREWFQSKDIPAEKDATKKIADHEQRRKYIPRVFLLGLVGFS
                     PQAGLFLFTVKILDPGIASLLMYLYPSFVFLILFVWRHQRPKRIQILALALSFLGCIL
                     TFWKAGSYPFMGLALGILVAVAYAVYLVIVERVLVGVDSLWATAIIMTAAALVYLCLA
                     LLQGTFIIPSSTEQFIIVLCIAIIASVLPIVTLFIAMKNLGARDTSIISTIEPVFTNI
                     FSILLFGENLTIQRILGGVSILAGVLILNRWGYASHKKDSAADLSGPSSPLSKSPGTE
                     EGKL"
     gene            complement(17706..19220)
                     /locus_tag="PK105_01755"
     CDS             complement(17706..19220)
                     /locus_tag="PK105_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60461.1"
                     /translation="MSETKILDYNKQEVRDQLLRALRSHTKESTSADLARATGLPLAQ
                     INAEMPAISDEYRGRIRVSERGDLLYSFPNGFKSRYKGFGPSLRRLWKTITRGAVKTG
                     KLLFKVWILVTLFGYFFLFLALALIALFASVMMRAGGSSDSNDRRGGGGLGGLWLTTN
                     LFDSMIRLWFYSELFKGPETRYRGTRAKAQRKPLYKAVFSHVFGDGDPNAEWDTILKK
                     AFVAFVQTHKGVITLPEFMAISGLKPEEAEDCITRYMVEFEGSPEVTDNGALYFFFPS
                     LLAKATTVSGAAASTFPIKKLHRFSSNPEKLDRTFRWVNIFNLVFGSYYLWNAINLGT
                     AIIIQTPEGQGLKGGLSFLYSYTVYLFSVLGANQPAVLVGWVLGVAPLAFSLLFFGIP
                     IVRSQRIKRENETIKRENMRRVLYSSVIADPNYFDPQSLQLPANEVRPEKADAVLEET
                     QHLAAWSRGEISPEGKWSFGDIEFIEREAERVRASIEERDYSPQKTVFDTDSPI"
     gene            complement(19266..20738)
                     /gene="glgA"
                     /locus_tag="PK105_01760"
     CDS             complement(19266..20738)
                     /gene="glgA"
                     /locus_tag="PK105_01760"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOU60462.1"
                     /translation="MKILFISSELTPFAKSGGLGDAVSSLAAALSKLGHDVRVLLPRY
                     YFISKNILRRINGLLEIDSARGRHRAFLYTTNLPNSQVKVYFLECESLYGRNGIYGYS
                     SNQEFPDNPERFSVLSRSAFAVCRNLGWIPDIMQAHDWPTAPVPVYLNTIEKNTEFSG
                     TAGVLTIHNLGYQGIYGRDHFAELGLGWEYYYGAGFEYYGNINFLKAGIVSAECIATV
                     SPTYAMEIQTPSGGFGLDSLLRQRSRDLVGILNGIDTEIWNPSKDPYLPAHYSVEDIA
                     GKAICKESLQRELGLPIEPEVPLIGMVTRLVEQKGISEVFGRTYGCMRKILETIHVQV
                     AVLGSGDAWAEEAIVNFSAQYPKFKGVIGYNERLAHLIEAGSDFFLMPSRYEPCGLNQ
                     MYSLVYGTLPIVHRTGGLADTVVNYNQNLGDGTGFMFDDLTPQAVYDTVGWAMWAWYN
                     KPEHIKTMRERAMKQDFSWNRSAEEYIRLYQHALAIRKAR"
     gene            20950..23220
                     /locus_tag="PK105_01765"
     CDS             20950..23220
                     /locus_tag="PK105_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60463.1"
                     /translation="MTERRIFLSLGAVIFITAFLFIACPSGIPKGNGYVYIRCVLPGN
                     LTEGNTRKTQSRLIGPESGWNIASYRLTFTSENGEVITKSIGKTGAELSLKAGNWSLL
                     AEGLTADGAIIVKKETQVLAQAGKRVNIQIMMHLAQGKGSLQLALSPNQPLPQDWKYS
                     LSLEYRGLPGDPTFQGPAPLSTDVPATQMDYQVNELESGNYSLAIQVKDTSSTSIAGC
                     ITTVLILPEQTSRGSCSISLPDPSATISLSTPKLELSADAAISVERYLNRNKPISVPF
                     VIAQGEADLDVQWYQNGTIIEETQAEADQSLPGYKTFFSPDKQPDTQMRISMEVLLSE
                     GNSGISQAFSHSSSLLTGPSSSEIEWIQSIDYKAALNPSLHNSIYPENAGTGNLHEAK
                     SVATSPTGLIAVAGLDQASALHLFYSPYGEQATLDGNNRQIPSSIGWIRLWRDRIFIQ
                     KSEKNPDIASISMDGSMIAIAATAGKWIRVYRLENSGSISRKIDIISGENGAPTFENI
                     RAMRFTTDSTRLFILANSSGKLLVLDIEKLFRGEPCCEQEFSITGWLNRTSEGSQQNP
                     SETEHSSLGMQDMILLDDGWIAACSSTIKMVIFIRYSSSDCSFSEPIIFTSGSNSASL
                     GDPKSIAFDEGNERCYVIGAARKLHLFEKSDPLSAYSLSMTYSLSSDLNEANSLALVK
                     KNSGGAYLVAGGNHGLGIISLDTDGHPICQGSLNSQEDNLYGIKSINNVTVLGESVIS
                     AGGSSGIVAMFDILGL"
     gene            complement(23355..24797)
                     /gene="gcvPB"
                     /locus_tag="PK105_01770"
     CDS             complement(23355..24797)
                     /gene="gcvPB"
                     /locus_tag="PK105_01770"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOU60464.1"
                     /translation="MNEINMRDEPLVYELSKEGRIGCSLPRSDVPSTGMPPELARTEL
                     DLPELDELTVVRHFTRLSQKNFSIDTEFYPLGSCTMKYNPKANEVVASNPGWKNLHPL
                     AEEQYAQGAIELLWRLQEGLREVGGFAAASLQPAAGAHGELSGVLMIHAALKELGETE
                     RKKMLIPDSAHGTNPASCTMAGFTTQTIPSGPDGSIDMEALRAALDDTTAGIMITNPN
                     TLGLFERNIEDICRLVHEAGGFVYGDGANMNALTGVFKPGRSGIDVMHFNLHKTFSTP
                     HGGGGPGAGMVAANETLAPYLPGPIAIKEASGYRLKMPAKSIGRVKAFHGNFGVLVRA
                     YCYLLMTGADGLRQVAENAVLNANYLKSLLENDYPVPYKRHCMHEFVAKGTIAADVHT
                     LDIAKRLIDYGFHPPTIYFPLIVQEALMIEPTETESKETLDLFAEAMKKIAEEARTDP
                     ELLHDAPHIAPVARLDEVAAARHPILCYRS"
     gene            complement(24794..26113)
                     /gene="gcvPA"
                     /locus_tag="PK105_01775"
     CDS             complement(24794..26113)
                     /gene="gcvPA"
                     /locus_tag="PK105_01775"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOU60465.1"
                     /translation="MSFVPHTDLERAEMLKAIGIPSIEDLFSDIPEHLRIEDLALPES
                     ISEIEAIDQLETLASRNVQTKDMDWFLGAGAYYSYIPAVVPALAGRGEFLTAYTPYQP
                     EVSQGTLQAIFEYQSMTAKLLGMSVVNASHYDGATALAEAVLMAWKARENRNRIFLPK
                     DLHPEYSQVISTYCTSFDIEFIRYEGDPGQVAIDEDTAAIVVAYPGFSGQFYPVSKAS
                     DKAHEKGALCIVQADPLMCAIFKSPGEQGADIVTAEGQCLGNPLNFGGPYLGMMGTTD
                     ALIRRMPGRIVGQTKDKFGKRGFVLTLSTREQHIRREKAVSNICSNQGLSMLQTCIYL
                     ATLGKNGLQKVARLSYDKAHYAAQLIDSIPGYHVVPKLFFREFLVETPDAAAKIVEAL
                     NKKGIVPGLALSRYYPERKNELLICVTEMNTKAQIEKLAVALKEAAK"
     gene            complement(26118..26501)
                     /gene="gcvH"
                     /locus_tag="PK105_01780"
     CDS             complement(26118..26501)
                     /gene="gcvH"
                     /locus_tag="PK105_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOU60466.1"
                     /translation="MAIDKNARYLESHEYAKPEGELMVIGISDHAQAELGDVVFIEFP
                     AVGKELDKGVVFGTIESVKAASDLYMPISGKVVEVNEEVKNDPSLVNKDCYGSGWLIK
                     VAPSKLSEFESLLDAESYGASIGEV"
     gene            complement(26559..27638)
                     /gene="gcvT"
                     /locus_tag="PK105_01785"
     CDS             complement(26559..27638)
                     /gene="gcvT"
                     /locus_tag="PK105_01785"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOU60467.1"
                     /translation="MHDWHVSHGAKMAPFAGYDMPINYPTGAIEEHLITRRGVGLFDI
                     DHMGQFEITGDAAESFVSHMISARVSDLVAPMARYSLLLNEKGGIIDDLFVYRLQNSW
                     WIVVNASNRAVDLEWFRSHVPSNVSVVDRSDDTYMIAVQGPRAIELLDKISNTPVSPI
                     PRFCWANISIRGIPILFGRTGYTGEDGGELFFPAEKAIELWEYLLECGESYGIETRPI
                     GLAARDSLRFEAGLPLHGHEIGVDINPIEAGFRWACDFEKEFIGKDALLSIIEQGVRR
                     KLVGLEVFGGVAREGYEVATPDGDIIGRCVAGMYCPTVKKYAANAFVIPDYAKIGTKL
                     SVIIRGQPRSAEVIKRPFYVPVYRR"
     gene            complement(27724..28557)
                     /locus_tag="PK105_01790"
     CDS             complement(27724..28557)
                     /locus_tag="PK105_01790"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOU60468.1"
                     /translation="MAVRKKKRNQAKTGCLIWLGAFLILLILFLVKFEDIRTAVEKTG
                     FIDALNHAVSKQDSEPQQKPQETNPEEPAPAPTLPSAAPPAEEPQIQSPILPKETTTT
                     TKAPKEVELPSAPLSESEPIASSPGASISKLSKEEQKTRTAVLYFVYIGDDGSISSQK
                     VKRTLAISDSPLQDTLESLLKGPTESELRANLLSLIPADTKLRSVNIKGGTAILDFNE
                     AFAYNRYGKEGYLAQLKQIVYTLTEFQNINDVQFLIEGKSRAFLSEGIVLDKPWSRTS
                     F"
     gene            complement(28576..29628)
                     /locus_tag="PK105_01795"
     CDS             complement(28576..29628)
                     /locus_tag="PK105_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60469.1"
                     /translation="MTIRSEFRSSEPPAAEELEAAIDAGIAANLHVSAELRPSDPANS
                     LPGGLIVLPPDKPCILVPDLHARPSFLHALLLTEFPGLSEPLLSVLSKNNATLLFLGD
                     IPHAEGEEAARRWISSYDKALIARDVRALLSPEMDEEMGLSLTSLLKVIELQCGFPNS
                     VFCLKGNHDNMTNAGDHGDFPFYKYADEGKMGAIWLSFRYGPFIMHKLREYELSLAIA
                     AENLNFCASHAEPAQEITRDSIISYRSNSRIVQALIWTSNDEAEEGSVRKSLKSILGS
                     EDRARHSVWFSGHRPVSGHFALRAEGQLVQIHNPALWQVVLVDAESRRADFFTILPDQ
                     PKPKKAASLVLDPLAR"
     gene            complement(29645..30871)
                     /locus_tag="PK105_01800"
     CDS             complement(29645..30871)
                     /locus_tag="PK105_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU60470.1"
                     /translation="MKPPSRDKSRGNQKKGRANQDNRQLLPEAFEERKKQALSSILYI
                     RFEGADAQSRLPSGIDASIPYPVQLQAPAVKLDPSSITIESLLAGMLRVLAWDSENEN
                     ANKYRKYIKMSRPELFEELIVAGIQKAEEKDWIVAEEVFFAASGLEPERPEPYINLAL
                     MHEEHAKRLFDSGNEDEAEKEDDLAYKYYQYLLGFKDVFAPAYYHAAFFFLRKHNYDK
                     AVSLLTSYIGMNDDEQRTRRAKLILEKLGGMGYLDTLFKEAYDFIQMGQEEKGLEKAM
                     QFVEKYPDVWNGWFLVGWANRRLGLWSEGAQAFHTALEKGADGADVYNELSICEIEAG
                     DLEAARSHLERALRIEPENEKIIVNLGALAFRQGHIDEAEGFFRTSIELDPDDRIAKE
                     WIKKIQALRERKGAGA"
     gene            complement(30904..>31733)
                     /locus_tag="PK105_01805"
     CDS             complement(30904..>31733)
                     /locus_tag="PK105_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60471.1"
                     /translation="AYIMHLLVQRSSMEYRNNMIGFFIKKAFFDGWDNLYLLAALNGI
                     FLGILLLFLVVPIALAFPIWLSVIMAGFGILLLSIWDVVVTNAMYNIADGKNVHFADI
                     KSAIRRSLKLGSLLGGVNILIVVAIMIAIPFYLGQKAVLGIFIGGLLFWMMILATLIL
                     QYVPAVFAREGGTPRQAFKMALYLFIDNPGFSIFLFFWRILTFVISIFVVLLAPGPAG
                     SSLASAVAVRLRMRKYGYLNDNPGADRHHIPWDDLLQEEKERVGKRTLKGMIFPWKD"
BASE COUNT         8941 a         7409 c         6962 g         8421 t
ORIGIN      
        1 tgagtattgg tgcggctttt tttggtgagt cgatgtttag cctggagagc aatgcttcaa
       61 agattggatt tctatcttta gcgaggacca tattcgaccg gggctttgaa tttatcgatt
      121 gccaggtata cactccctac ctggaactga tgggaggcat tgatgtgcca cgttcgattt
      181 acctctctat gctcgatgct gcgcttaaaa agccaacgat aaggggagac tggtccaaga
      241 tgttcccctc ctttcctgaa agagagctaa ttatgtacac agataagaat cataaagtaa
      301 tggtagatag ctgatgatgt caaatcgaag atattggatc atcgatgata ggatcagcaa
      361 agccagaaga atggaacaac aatgaaattg ctttttgtac aattgcctgc acagatgccc
      421 gactggaatt cggtacctgc aaatgtccca ttagctgccg gatatctggc atcctatgcg
      481 gaatcaaaag gattgctcaa tcgtcgagaa tggacaatac ttgagcctcc catagcgaac
      541 tatgggtcag atgcttccat tattgcgtct attgttgtgc gcgagccaga tgttgttgca
      601 tttactctct attcatggaa tatagagcgc agcctcttcc tggccgaaaa actctcatca
      661 cagctgcctc gcgctcgatt gatagccggt ggcccggaag tcgtcaaacc aatgccaatt
      721 acatcgaaat cgccttttaa tagcctaata tgcggcgaag gagaagaagc atttgcggat
      781 atactgcaag atattctcga gcacaggcca atttcatctt tctatagttc agacaaactc
      841 atagacctca acaagttgcc aaatccatat ctgaatggcg ctctgctttt cgatccaaac
      901 tttcaggttc atatcgagac tatgcgggga tgcaattcaa aatgctcata ctgctattat
      961 gggaagaatt ataaaaccgt tcgctatttt ccgcatgaac agatcctgga agtaattcgc
     1021 cttgcctcgg aggcaggtat tccagaaatc tatataatgg accccaattt tcaaataagg
     1081 cctgattttg caggaaagct tcgagatatc gcatttgtaa atcatgcaca tacgtctatt
     1141 cataccgagc tcagactcga agatattgac gaagagatag ccggtcttct aaaagaagca
     1201 ggcattgtat cggtcgaagc gggacttcaa agcacaaatt ccaaggcttt ggaagctatt
     1261 cacagaagct tcgatagaaa ggcctttgaa cgcggggccg aacttctcca gagacagaaa
     1321 atcataataa agacaggtct catactcggc ttgccatacg atggctatga gcaggtcatt
     1381 gaaaccttcg attttcttgg aatgcagggg ctcggtcaag aagcagagtt gtatccactc
     1441 tcgctgcttc ctggaacaga gatcagagaa cgagctgacg agtatggcat gagtgcaatg
     1501 gaccctcctc catatctcgt aaccagcaca cactggatat cctatgatga tatggtcgat
     1561 gcgatttcgg cctttgagga gagcttcgat gtggagtggg caatgcctcc tgcacctcat
     1621 tttcaacttt ttaaagaagg attcgtctca ttcatcgatc tccgaaagcc cgagaatata
     1681 gattggatgc gtctcaatcc ggaaaaactg tcaaactcca tcactcttct ggtcgatgca
     1741 gacgaccccg aaatactgtc ccgagtagtc agagcagcca gagacctacg taaagacaat
     1801 ccctacacac tctatcaaat cgtgcttaca agcgagacaa gaataccttc cgagaaactt
     1861 gttgagagaa ttagggatgc gttcatgaac tctgctcact attatgagct catgaactca
     1921 ttttcccccg atccgcagac tagttatcaa accagaatat tctttgctac caagaacttt
     1981 acccttgcgt atcgagccct cgaagaagct caagacatgg agacaatggt gatcctaagc
     2041 ggaaagggtg gctacaattc ggatcgcctt gcggagcttc tgccttatgt gattttcgat
     2101 aaattatctc ttccttttga tcgactttac gaactcatat caatctacgc tgattttccg
     2161 catatgttga ttgaggcacc tgaaggactc ttctgagata ccaggctgat tgtctttata
     2221 attctgttct ataaggcctc aatacatctt agaaaggtca gcctgccaga taccgcgccc
     2281 tgtcctgagg taagatctaa tgttagccac tgtgtcttcg atcgatttag ccgaggctcg
     2341 atttgtcgat cctcccacat ggggggaaag caccacattg gaaagtttat gtataggata
     2401 acgcgaaggc gcacccacta cacctgtggc agggtatgta taccatgtat cgatggcggc
     2461 acctttcagc actccattct ttagagcttc gtacaagcct tcttcgttca cgatcgaacc
     2521 tctgcccaca ttgaccagga atttcccctt catgttcatg agcatctctt ttgagataag
     2581 attctcggtc tctttagtag ccggaagcgc tatgaagata atctcggcaa aatcgatggc
     2641 ttcctgcaaa gtgggcacta tggaatcgaa atgagctggt tttttggcgt cttttgtcct
     2701 gcgccagcca tataccgtgc aatgaaatgc cttgagaaga ccagccagct cctgaccaat
     2761 ggctcctgtc cccagaatcg aacaattccg accatataga gtatcccagt tgtcttctgc
     2821 tcctcgatta acccaaaaac catgccatat ctcatttttg aggtcattgt ggtactcgat
     2881 gacccttcca taaaaagcaa gagttagggc aagagctttt tcggcgacat cgaaggcatt
     2941 ggaatgaaca ttatatacct gaatattgcg ctctaacatc aattgagaag gcagatgatt
     3001 tatccccgta aatggctgaa acagggcttt aaggtttgtc gctgctttga aaacatcggc
     3061 tggaatacgc ccagcaatga ttagatccag cgaacctatt tcggaggaat ctgaggtgag
     3121 cccttccaac catgtgtgat caggaaattc ttctctcagc tgttctatga tggttcgcca
     3181 gatagactga atcggcatga aggatccaat tttcatgatg gcctctctta aaataaacta
     3241 tatcatatat atggaagcat acttggttcc taaatgcaag caaccttatg aaattatcaa
     3301 ctttttgcga tataaattgt gaagactagg gatttgtatt aatgatgccg atattcttga
     3361 agggagatat tgagaatgca aagacgatct tttaaggtag gacgattttt ctccgcgatt
     3421 attgccgtgc tatttctgct tggaggttgc gcgacctccc ctgcaaaaaa taaagaggtc
     3481 actctaaacg atctagtaat ccagggtgat tttgaaggta tacgtaagtt ctattcaaac
     3541 caagaggaat tgaacaaacc ttcaaataaa gaaccctatg tcgggcttta tcctcttcat
     3601 atcgcagtaa cccaaggcaa tgctcagata gcagagattc taattgtgct gggtgctaaa
     3661 cccgataatc aggatctctc tggaaaaaca gctttacgat atgcagtgga cagaaaacag
     3721 acagcaatgg taaagatgct catcgataga ggggctgatc cttttatcac agatgtgggc
     3781 gggacttctc cagccctggt agcgctacac tcagacatta gccttctcga tgcaatgttc
     3841 aactctcgaa atatcaataa caccggtccc gatggaagaa ccattctgca ccatgcttcc
     3901 gatgcactaa tgccaaaaga agtcgaccta cttctatcca aaggtgccct tgtacagata
     3961 aaagataagg cagacaggac cgccctcgat ctcgctctcc tttatccgga gaaactggaa
     4021 gcagcccaga tagccgagaa gctgattcta aagggggcaa atccttcttt cccagagttt
     4081 tcgtggtttg cggataccgt gcggaggcct gattatggtg ctgcccgctt tgctaatggc
     4141 aacacgcctt tgcatgaggc tatctcccga tatcaacatg gttttgcgct ttttctattg
     4201 ctcaataatg tcgatcccaa tctcaagaat ttaaatggag atgcatccct tcacctggcg
     4261 gtaaagatgg gatatgtcga gggtgcaaga cttctgctct ctcataatgc aaatccagat
     4321 attctcgatg ctaatcagag cacgcctctg catctcaccg ttccagctac acagcgtctt
     4381 gaaatggtca aacttctctt agcatttaag gccaaccctg cactaatgga tatccaagga
     4441 aatacgccac tccataaggc agtagcgcag ttatatgctc ccgaaatagt cgaaaccctt
     4501 attcaagcag gcgcccctgt aaatgcacag aattccaaag gagacacacc tcttattata
     4561 tgcgccagga ccgagcatta tgaatatgca aggcctctaa tcgaagctaa tgcggaaatc
     4621 ttcaagatga accaagcagg cgaaaatgct ctcaagattg cattgagtaa aggctatact
     4681 gccgtggaca atatcgtact gccatctaat gtgaatcaga gcgatgataa ctctaacagt
     4741 gttctcatga ctgctgtatc gcttaaagct agcccggatg ttctcaagct gatactctct
     4801 aagggagctg acccgaacgc taagaacaaa gcccaagata ctgctttgca tatttctgtc
     4861 agacaaaact atgctcagca gggtatcgtg ctattggatg ccaatgcaga catatttcaa
     4921 tataacgcgg tccaagagaa tcccctgttt ctcgcactca ccgcaaaacc cgcacctttg
     4981 gaatggtttt tcaggccaaa tgtgataacc gctacggatg caaatggaga ttctgtagtc
     5041 caccatgctg cccgcaagaa tcttcccgaa ggaatttctt acctcgtcaa aaagggagca
     5101 aatatcgaag cactcaataa tgcaggagag attccacttc atacggctgc gaaatacgat
     5161 gctactgatg caattcgtta tctggtaagc gcggggtctt cattgaatgc agcaaataag
     5221 aatggcgata tccctctcca aagcgcggta ttgaatggag ctttcaaagc aactcagatg
     5281 ctccttgcag caggctctcc agtgaataat cagaattatt ctggcgagac agcattgcat
     5341 caagccagca agaacaacag cgccatgatt atccagctac ttacagctca aggtgcacag
     5401 accgagattc aagacagtcg cggttttacc ccactcataa ccgcagcggc caatgatcgc
     5461 tatgatgccg caagtgaact tatcaaggct aaggccgtca tcgatacgcg tgactacctt
     5521 ggatccacac ctctatatca tgccgtaagt ggtgaatatg ccgcattgat aagattgttc
     5581 ttggtaaacg atgccgatat tcatgctctg aatgctcaag aactctctcc gtttaaatta
     5641 tcactttcga agaatccaca gataactccc gaattctata cagataagct tataaacaag
     5701 cccgattcca gcggagattg tgttctgcat atactagtta agagtggaca agggcaggaa
     5761 gctcttacgc aagcactcaa acatggagcc aatcttaatg cgagaaactc aaaaggagat
     5821 actcctctta tgctcgctct taaaaagggt gatcttaacg cagcggtggt gctaatacaa
     5881 gcaggatcat ctctctttgt atcgaatgcc gacaataaaa tacctctttc tgcgatcttt
     5941 ggaatggatt cgggcgctag gatgaaactg ttctcagcca ctggcgcaaa cagtgcggat
     6001 tatataggcg aatcaatact tcactatgcg gtaagggcga ataatgcgga tgttgtgtcg
     6061 gaattgctgt ctctgggagc ggatagaaat gcgcacaacc gcaaaggaga gactccaaaa
     6121 gatattgcgc aagcaaaagg atatcaggag attcttgcca tactagaacg caaataaaat
     6181 gatcatgatg ctaattatca agatggcata ctgatttaac atatccagac ctacaagcta
     6241 ggactccatc ggcttgtagg tcttttgctg cttatagcaa ggatatcaat cctttatgga
     6301 atggcttcaa ggtcatattc actggattga gtctgaaagt tcccgtctat tcctcatctt
     6361 gtcaagagct ctttcttgaa tctggcgcac tctctcacga gttattccga gagctaagcc
     6421 cgttgcctca agagtttttg gcgcttcgcc attcagacca tatctaagct ctagaacagc
     6481 ttgttcccgc tccgaaagag aatccatgac ataactcagc aattctctta aggtaaccag
     6541 gaatgcatcg ctataaggat cggacgatcc ttcatccttg agattgttta ccgggcttgt
     6601 gttctggttc tcctttgtac atagatcaag gctggatgtt ccgagcgcca ggagtaaagc
     6661 actctcgacc ttgctcaaag ctataccgga tctttcggct atttctatgg agttgggctc
     6721 acggccaagt tcctggacaa ggcctttagc cgatgcataa cattggcgga tcgtgttcaa
     6781 catgtgaatc ggaagacgta tcatcctgct ttgatccaca agacacttga ttatggcctg
     6841 ccttatccac caacttgcat aggttgagaa acgataccct tttttataat cgaaacggtt
     6901 tactgcttcg atgaggccaa tattgccttc atcgatgaga tctaagagac ttacgcctct
     6961 cattcgatat cccttggcta tggaaactac caagcgaaga ttagaagtta tcagaacttc
     7021 ctttgacgct cttatctttt caagtgtgtt tctcctcatc tccaaaagcg gtatattttg
     7081 agggtcttta gttatttctt tatcatagtt ctttagcaaa aggccaagct cctcaagctt
     7141 gacaccgatt tcttgctctt gttttgcatc gagaagtgga aacctggcaa tttgtctaag
     7201 atataagctc agcggatcgg attcctgctc tgctttttct tgagagcctt ttcttgaggg
     7261 taacttagaa atgcttgctt tatcgcaatc caagtagtca cccatatcca ttccttgtcg
     7321 aattggctaa ttcggcaatc aaaaccaata gaattttacc ttattgtatt tgaacgcaca
     7381 agatgaaagt caatagtgct aatagtgctt tattgtcagt ctcgtggagc aagcgaaggg
     7441 tcgacaggtt ggccattacg aaagacaaag aaatatgcta tcggattgcc atcatttgca
     7501 tctataccaa cctttgcaat ggcctttccg ggttctataa tttctccggt ttttacaaga
     7561 atagactcat tgcctgcata tacatagaca aaaccagctt tagattgtac gaacaccact
     7621 tcgccataac cacgagatgg ccctgcagat actaccgtac ctgaagctac agcctttacc
     7681 gacgcacctc gcgcggtctg aaacatcacg ccttcgagct tcccgctcat atactgagct
     7741 tttccatcta ctggccagtt tacgattgca tctggaatgc gtttacctga tacgctagtt
     7801 ttgggactat cggccttggt tggggcggaa gtctgagccg gtggatttgt agattgtgaa
     7861 gctgcagacg atgatgttgt aggggaattt ggagggagaa cggcctgaga agttgaagat
     7921 gtggttgaaa cagaattgga tgggacaata gcggtatttg caggggaaga tgaattctgc
     7981 tgagtggaag ctgaagcaac gggctggctc atggcaacgg aatctacaac ggaatctaaa
     8041 ggaggcgagt cttgaactgt ctgtacagaa atgactcctt tttccgggat gattaggaca
     8101 tctccgactt tgagcacata gatgtcggag aggttgttgg ctgatttcaa ctcttgaatt
     8161 gagataccat attgcttaga aagaccataa tatgtctcac cttttgcaac aatgtggact
     8221 cgggggatta ggagaactac tcccggacga atcttggacg gatcagttat gctattgata
     8281 ctggcaagcg tttcatatgg cacagaatag ctcttggcaa tcgagtatag cgtttcgcct
     8341 gcctgaataa catgatattc ctgttctgca ccgctgattg tgagaagcaa accaaaaagc
     8401 aaaaagaata tggaggcttt tagaattcgt ctaaccatag gaagttccaa ttaatttatc
     8461 ggcatgcttt attcgtcatt gaagaagaaa tttgtcatta atgccctttt cattcaaaag
     8521 ccaaattgtt agaatgtctt cgatattcta tgcgagaggt atattcagat gttcgaaaca
     8581 tcaccgctac atgctggcag gtattcttat gttccaaaaa tgcctgagac atttctagat
     8641 tcaatccaaa gcataaagac tatccctacc gaaaccatag aacctgagag agatagggaa
     8701 gtgctaaaga agttcttccc taacagttat ggcaagcccg cgataaaatt cgtaaagggc
     8761 gagggggctt tagaagataa agctattact gtcggggtca ttctttccgg tggaccagct
     8821 ccaggcgggc ataatgtcat agcaggtctt ttcgatgcgt tgataaaagc aaatcctcaa
     8881 tctaggcttc ttggattcaa gagtgggcca tcaggcctta taaaaggcca atatgttgaa
     8941 ctcagctctg agcttatcga tcagtatagg aatacaggcg gattcgatat gataggtaca
     9001 gggagaacca agatcgagac ttcagagcag ttcgaacaat ccttggagaa tgcaataaag
     9061 ctggggctcg acgcccttgt tataatcgga ggggatgatt ccaatactaa cgccgcactt
     9121 ctttcggagt attttttcca aaaggaagct ccaatcacag tagtcggtgt ccctaagacc
     9181 atcgatggag acctcaagaa tgaatggatc gaaacttctt tcggcttcga caccgcaacc
     9241 agggtatatt ccgagctgat tggcaatatc tgcaaagatg cttcgtcggg tcgcaagtat
     9301 tggcattttg tcaagctgat ggggcgatcg gcttcgcata tcactctgga atgtgccttt
     9361 caaacaaggc caaatgtggc tctcatatcc gaagaggtcg atgcaaagaa gatgagcctg
     9421 tctcaagttg tcgaaatcat agcaaacaca atagctgcgc gatcggcaaa aggcgagaat
     9481 ttcggggtcg tagtcgtgcc agaaggcctt atagaattca ttccggaaat caagacactg
     9541 attgctcaga tcaacgacat tatcgcaaca tccgaagatg aatatgagtc cttgcccaaa
     9601 ggagaagcac agagatccta tatcctatcg catttgcctt cagagtgtat ttcgttgttc
     9661 tctagccttc cagaatcgat tcaaacccaa ctttgctggg atagagaccc tcacggaaat
     9721 gtacaagtct ccagaattga aaccgaaaaa ctactcgctg agatgacttc tcgcaaactt
     9781 caagaaatga ctgctgttgg agcatacaag gcaaaattcg attaccagac gcacttcttt
     9841 gggtacgaag gccgctgcgc ttttcccacc aatttcgatg cggattattg ttattctctg
     9901 ggatatgatg catgtctcct cattctgcac ggattgacgg gctatattgc tgctataaga
     9961 aatcttgcag ccgctccaaa ggattggctt cctggaggaa ttcctattgt ctcattgatg
    10021 aacatcgagc accggcacgc agaagaaaaa cctgtcatca aaaaggcgct ggtggaactc
    10081 gagggaaagc cattcaaagc attctctgca atgcgagata agtgggctct ctcatcagac
    10141 tatcggattc ctggcagcat ccagtatttt ggtccccctt cgattaccga tactccgtct
    10201 ataactctgc agttggagca acgcttgtga aaaggaaaca tattaccatt attgcttcta
    10261 ttttcgcttg ttttcttgga gcaagcacat tgagtgccca ggtattgctt accgcagatc
    10321 agttcttttc gcaattagcc gaaaaatatg caatggtaac tgattatcaa gcggatgtgc
    10381 atataactgc cggtcatcaa ccgatggcag gaactttgct cttcaaatcg ccttctctat
    10441 tgcgcattga tttttctcag ccagccgatc aggtcattgt gttcgatggt aaaacgctcc
    10501 tggtttatct tcctcaatat agagcagtgc ttcgccagga ttcgaccgat cagggcattt
    10561 ccgttggagc agcgactctc gcttcaaaag aaggtctctc gctgcttagg cgaaattata
    10621 ccataggatg ggagcaaagc cctaatcccg aaccactgga cccgggctcg gatgagctgg
    10681 tttatcgcct catgctgtct agaaaaagcg tgtctgaggg atacaagaac atacggattt
    10741 cggtaagcgc cgatactatg cttattcgga gactagaagg ctggaccgtc tcgaatgata
    10801 agattacctt tgattttgtg aatatacaat tgaaccgagg catcagcgac aacaaattta
    10861 tctataatgc gcctgcgaat gcaaatgtct acagcaattt tctgtttcag taagaaaagc
    10921 caaggacgcg gggagaagtg atgggtaaga tcggtgattt attccgtgaa gctcgccttg
    10981 gaaaagggtt gacgctggag caagtagccg acgagaccaa tatcagcaag cactttttac
    11041 aaggaatcga gagtgatgat ttcagtgatt tccccggtga ggtatacagt cttggcttta
    11101 taagaaatta tgccgagttc ctgggcctcg atgcatcggc catggtggag atttatcgat
    11161 caaatgagag cacaagacaa gccgaagtcg aaaaatctga aagcactgag ttacctttgc
    11221 ctgttgaaga tagcaaaaaa gaagaaactt tgcctcagaa atcctttatt tcatccgaag
    11281 tcgaaaaatc cgcaaaagag actaagcaaa ccttccatgc gaagagaaaa ccaaaagaaa
    11341 aatccattaa gagagaagaa ccagaaacca tcaaagcaga gccaaaagcc tccattcaag
    11401 tggatagtac tttggaaaaa gctgccctta tcgattttca gccaaagaag aaaatcggta
    11461 ctgccaatat tcgggtaggc agagtattgc cattcgtttt tgtcgctata gtgcttatta
    11521 tcgcagctgt cagcattata cccagactca gaatatctcc tggcgccgct cgagttccaa
    11581 ccgagtatcg cgccgaaggt ctgcctttcg agcagcgcct ctatccgcag gacaaggtat
    11641 atatccctct cagcggagat gtcatctcta ttaccctcga atccatcaaa gataaggtct
    11701 cattcaatac tccatttgga agcttagcgg ttggattgaa tgaaaaaaca gtagtggacc
    11761 cttccgccga tagagaacgg ctcgttattg cggtgagcga ttatgaggcg gataaatctc
    11821 agaatggggc catggttcat ttcgatgtcg aagaagccct ttccgatgct gagaattcat
    11881 tggatatcat tgtgccgtca acaccgggaa aagctcagct ttcccaagat tcgacacaag
    11941 gccaagcagg tggggcaaac aggacacaga catcttcgac agtacttttc agaagcaccg
    12001 gtggacccca tcctttttat gttaatgtga gtttcatagc gccggtcttt ttccgttacg
    12061 aagccgacag aaaagaatgg gtcgaaaaat actaccgcaa gggagaatcc ataacggtaa
    12121 acgcatctaa taccataact ttttggacgg caaatgctca agctgtaaaa gtgaatgtat
    12181 ttcaatcagc aggtaagtcc gtggagcttg tgatgggagg gcctggagaa atcgcggttc
    12241 aacgcctgtc ctggtcgaat tcgcaaagtg gatgggcaat gatagcaact caggtggatt
    12301 aaagctcttg ctgtcaataa tccttttatt ctatgtatat catgcaagat cttgaggaaa
    12361 tcttatatgc ctaccttttt cgtagatcaa catggctgtg caaaaaatca agtcgatggg
    12421 gaagaaataa gttcgcgcct catcaatgca gggtttctag ccgcaagctc acccgatgag
    12481 gccgatgcta ttattataaa tacatgcggc ttcattgaag atgccaaaaa agaatccata
    12541 gcggcaatag tcgaagcaaa gactagattg ccgaaaaaaa aagtaatcgc aatcggttgc
    12601 ctctcgcaac gctatcctga agaactattt aatggcctgc cagaagcaga tggcgtgctg
    12661 ggaaatggaa atctctccat aatcccagag gcggtgacta agattctaag gggagaacga
    12721 attgtgcttg ccactgccca gaataagaaa atgcccagcc catactatcc acgaatagtc
    12781 tttttcgatt atccgggaac agcgcatatc aaaatcacag aaggatgttc gaattattgc
    12841 agttactgcg cgattccact gattaggggt gagcttcgct caaggagcat cgaagatatc
    12901 gtattggaag ctcggaatct cattgcacaa ggcatgtttg agcttgtcct aattggccaa
    12961 gatcttggca attacgggaa ggatttgtca ggtcgatgct tgcttatgga cttgatggaa
    13021 gctctatcga acatagaggc agattttaga atacggtttc tctatattca tcctgaccat
    13081 tttccttctg agatattaaa gatcatagcg catgattctc gcttctcgcc ttatttcgat
    13141 ctgccttttc agcatgcttc gactccaatt ctccaaaaaa tgaatcgcca tggttctgct
    13201 acagaatatt tgaatctaat atacagaata agaagtgagc tgcccatgtc tatgattcgt
    13261 tccacttttc ttgttggatt tcctggagag accgaagagg attttgctgc cctgctcaat
    13321 ttccaaaaga ccgcagagat cgattgggtt ggtgtgttta catattcgct tgaagagaat
    13381 acacccgctt atgacatgaa ggctcgcatc ccaaaatcca tcgcaaggaa aagaaaagca
    13441 attctagaaa atgaacaaat tgcaataacc gccaacagat tgcagcgctt tatcggagtt
    13501 agcgatacct tcatcataga agaggcattc gatcatgatg agctgtgctt cggacgcggc
    13561 tggatgcaag ccccagatat cgatggtgta actataataa atgcatctct aaaacctggc
    13621 acaatagcga aggcgaacat tgtttctgtc aatggtgtcg atttcaatgc agaattagag
    13681 tatactacga caccatgata ttaggacaga ttcctgagta tctggaagga ttaaacccag
    13741 aacagcttga agcagtctgc tatgaaggaa accgtcttct catccttgcg ggtgcaggaa
    13801 caggcaaaac cagagttatc acaaccaaga tagcgcacct ggtcaaacaa agcaggtttt
    13861 tgcctgaatc cattcttgca gtaacattta caaacaaggc tgcaaatgaa atgcgcgagc
    13921 gggctatggc aatcgagcct tcatgtgaaa gagcgataat aaggactttc cattcctttg
    13981 gagcatggtt catgaggcgc aacgccaatg ccttcgattt gcgaagcgaa ttttccatct
    14041 atgatgaaga tgattcggcc gagcttgttc attcgatatt tcctgctttt acaaagcgcg
    14101 attgtggcga ttatgctttt ctgatcgcaa aggctaaaga ttattgcctt tcgcctgatt
    14161 ccccgaacct ggattttata agcagacatc cggaattcag acgcatctat tcagcttatg
    14221 aagataggct acgttccaca ggtaatgtgg attttggcga cctaatcttg atgcccgcat
    14281 tgttgctcga gaaagatgaa acaattcgaa atcgcacacg ccagcgcttt agagtaatcc
    14341 ttgtcgatga atatcaggat tcgaacatag ctcaatttag gcttttgcag cagcttgctg
    14401 gccctgattc gatgctttgt gtagttgggg atgacgacca gtctatatac cgtttcagag
    14461 gcgcagaagt caagaatata ctgaattttc ctaaagtctt tgcagataca aggataatca
    14521 ggcttgagcg taactataga tcgcatcaag ctattttgga tctcgcccac agcatagtag
    14581 tacacaacga aaacaggctt ggaaagaatc tgctagctac cagaccagga ggaaataagc
    14641 caaaaatcgc atttctcgat gaccaggatc aagaaattga gttctgtgct catatagcgc
    14701 gccagcatgt caaaaatgaa ggcgaatgga aagacatcgc tatactctat cgaacaaatg
    14761 cccagtctct aggctttgaa cgccttttcc cacaatttga tattccttat cgaatcgttg
    14821 gtgctgtccg attctatgaa cgcgaggaga tcaaggatat gctagcgttt ttggctatcc
    14881 ttgcaaatcc aagagatgag atttccttca aacgcgtcgt aaataagcct tcaagaggaa
    14941 ttggtgcttc atctctggat aagattatct cttttgcgtt ccataatggc cttgatctga
    15001 tagaagcttc gcgtcaatca attgcaagca tttctcgtaa aggaagcgaa ggtctcaaag
    15061 aatttctcag ccttatagaa gaagctaagg cttcacttca tagagacttg gctgatcaaa
    15121 aaaatccagg cgacctgggg tcgcttattt ccgattttgc tcaaaaaagc ggtcttctcg
    15181 agttctatgc cagcaaagat agaatatcga gtacccataa aaaagagaac atagaagaac
    15241 tcgtcaacgc agcatcagac catccgcttt cttcggaagg ccttctggaa ttcctggatg
    15301 atgtcatgct cgatcaatct cgacagaaaa atcaagacaa tgcagatgcg gtgacattga
    15361 tcaccatgca caacacgaaa ggcctcgaat ttcccatagt aatagttaca gggctcgaac
    15421 aagggctctt cccacggaac gacgaagagg gtgatgacat agaagagcaa aggcgcctct
    15481 tctatgtctc tgtcacaagg gcaaaaaacc agctttatct gacttcatgt cgttggcgcc
    15541 gaatccatgg aaagctcttt gagaccatgc cgagtcaatt cttatcggaa atcgaccctt
    15601 ctctatataa gatgtggggc gattccagaa ataagatgag agcagtccct tcacaatatc
    15661 gttcatcatc cacggttgct gtttcttctc aatccgatac atcgaccaag aaatccgaat
    15721 ggacagcggg tatgtctgtc tatcatgatg aatacggcaa tggaaccgtt ataaaagtaa
    15781 caccgtctca gagtgcaggt cctcttgtaa tagtccagtt tgagacagga aagactgcgc
    15841 aattcttccc aaaatacaca aaaaaattgg agagaatcaa gaattgaagt cgagaataag
    15901 aggggaacct tccggtatgc gcgacaaagc cagtgaagaa agcattttgc aacccaacac
    15961 cgattctctt ctctcgcatc tacccgaaat aaaccggatg cgaggctggc gaatgtatgc
    16021 tcgaaatggt tcgagaattc tgagtctttg gtcagactat ggcaggacac ttctaggtta
    16081 tagtccacgc ggccttgtct caattgcaaa aagttccatg gacagggggc ttttttcagc
    16141 ggtaccggga atatggaata gccgtatcga aaaaatgctg catagacttt tgccggaata
    16201 tcgcaatatc aaaattctag gctcacaaga aaaggcgatc aaattactgc aaaatctgga
    16261 agggcttgct tcttccactc caagcattgg cacttgcaaa ttcctctttg ataaatatct
    16321 aaaaagagat gacgagcttt cggatattat tagtattgtc ctgcctattc ctgattttct
    16381 atcgatcgga attctcgcaa gcaagatgtc ctcaagtcct gtaattgaac aagagcttat
    16441 cgagggcata aaaggcatga cttcgatcta tgctctatct aagatgcaat ctgaagaaga
    16501 aggtgtaatt tctggaggac tagagcctgt atgggaaagc ttcgaccgat atggaggagt
    16561 actcttttcc cgttctggtc catggctttt cccaaactat ccaagggaga atcatgaaga
    16621 ggtttttcta tactgtcttg aacatggctt gctcatctct ccggattaca attttccttc
    16681 ctctgtgcct ggggacttcg ataagggcga acttggcccg ctgagatctg ccgcagaatc
    16741 ctttttatga gatgcataac cccatctgtt gagaatcaag acccctgcaa gaatagaaac
    16801 accccctaag attctctgaa tcgtcaagtt ttctccaaat agcaatatag agaaaatatt
    16861 tgtgaatact ggctcaatag tgctgataat agaagtatct ctagcaccta gattcttcat
    16921 cgcaatgaaa agcgtcacaa tgggcaagac agaagcaata attgcaatac aaagaactat
    16981 gataaattgc tcggtagaag aaggaatgat aaaagtacct tgtagaagag caagacagag
    17041 gtatacaagc gcggctgccg tcataattat agcagtagcc catagagagt ccactccaac
    17101 aagaacacgc tctacaataa cgagataaac agcataagcg accgctacca aaatccccaa
    17161 tgcaagaccc ataaacggat atgatcctgc cttccaaaaa gtgagtatgc atcccagaaa
    17221 agaaagcgca agagcaagga tttggatgcg cttgggccgt tgatgtcgcc aaacgaatag
    17281 gattagaaat acaaaagaag gatatagata catcaacaag cttgcaatac cggggtccag
    17341 gattttaacg gtgaagagga agagcccagc ttgtggcgag aaaccaacga gaccaagcaa
    17401 aaagacgcgt gggatatatt ttcgccgctg ctcatgatca gcgatctttt ttgttgcatc
    17461 tttttcagct ggaatatcct tcgactggaa ccattcacga atatatacta cgagccataa
    17521 aaaaagggct gctacgagaa aacgccatgc gagcacacat aatacggaaa acccatgcga
    17581 atagagcaat tcggcaaaaa tgccaagcgt agaaaagctg atggctgaga tcatggccgc
    17641 aatgaaccca cgcattgcgg tactattacg agctcttgtc attgtgctaa atctcaagat
    17701 tactactata ttggagaatc tgtatcgaag accgtcttct gtggagaata atctctctct
    17761 tcgatcgatg cgcggactcg ttcggcttcc cgttcgatga attcaatatc accaaagctc
    17821 cacttacctt cagggctgat ttcccccctg ctccaggctg caagatgctg tgtctcttcc
    17881 agcactgcat cggctttttc aggacgaact tcattggctg gcaattgcag agactgagga
    17941 tcaaagtagt tggggtcagc aatcacagat gaataaagaa ctcttctcat attttcgcgt
    18001 tttatcgtct cattttcgcg cttgattcgc tgagatcgaa cgatcggtat cccaaaaaag
    18061 agtagagaaa aagccagcgg tgcaacgccc agcacccatc ctacaagaac cgcgggctgg
    18121 ttcgccccca acacagaaaa taaatagact gtgtaagaat acaaaaagct tagccctcct
    18181 tttaaccctt gtccctctgg agtctgaatt ataattgcag tacctaggtt tatggcattc
    18241 catagataat atgagccaaa tacaaggttg aatatgttta cccagcggaa tgttcgatcc
    18301 aatttttctg ggttcgaaga aaagcgatgg agctttttaa tcgggaacgt ggaggccgcg
    18361 gcaccggaca ccgtagtggc ctttgcaagc aatgaaggaa agaaaaagta gagtgctcca
    18421 ttatctgtaa cctcagggct gccttcgaat tccaccatgt atcttgttat gcaatcctcg
    18481 gcctcctctg gctttagccc agatattgcc atgaattcgg gcaatgtaat tacaccttta
    18541 tgggtctgaa cgaaggccac aaatgccttt ttcaagatgg tatcccattc tgcatttgga
    18601 tcaccatctc cgaaaacatg cgaaaataca gccttatata gaggtttccg ctgggcttta
    18661 gctctggtgc ctcgatatct ggtctctggt cctttgaata gctcactata gaaccagagt
    18721 cgaatcatag aatcaaagag attagtagtg agccataaac caccaagtcc accgcctccc
    18781 cgccgatcat tactatcgct cgagcctccc gctcgcatca tgaccgaggc aaagagtgca
    18841 attagagcaa gggccagaaa aagaaagaag tagccaaaga gagtcaccag aatccacact
    18901 ttaaaaagta attttcctgt ttttacggct cccctcgtaa tggtcttcca caatcttcgc
    18961 agagaaggcc caaagccctt gtatctgctc ttaaatccat tgggaaagga atacaggaga
    19021 tcacctctct ccgatacccg aattctgcct ctatattcat cggaaatagc aggcatttcg
    19081 gcatttatct gtgccagagg caagcccgtt gctcgagcaa gatctgcgct cgtcgattcc
    19141 tttgtatggc tgcgcagagc acgcagaagc tgatctctca cctcttgctt gttgtaatcc
    19201 agtattttag tttcagacat caatgtattc cttttaaatt atccgtagag ccttctttat
    19261 aatgctcacc tggcctttct tatggctaag gcatgctgat aaagccttat atattcttcg
    19321 gctgaacgat tccatgaaaa atcctgtttc attgctcgct cgcgcatagt ctttatatgc
    19381 tcaggtttgt tataccacgc ccacattgcc cagcccacag tatcgtatac cgcttgcggg
    19441 gttaaatcat cgaacataaa acctgtgcca tcgcccaaat tttgattata attgaccacg
    19501 gtgtcggcta aaccacctgt cctgtggaca atcggcaaag tcccatatac gagcgaatac
    19561 atctggttca gtccacaggg ttcatatctg cttggcatga ggaaaaaatc actccccgcc
    19621 tcaatgagat gtgcaagcct ttcgttgtat ccaatgacac ctttgaattt cggatactgg
    19681 gcggaaaaat tcacaatagc ctcttctgcc caagcatctc cggatccaag caccgcgacc
    19741 tgaacatgga tcgtctccag tatcttgcgc atacatccgt atgttcgacc aaatacttcc
    19801 gaaatccctt tttgctccac tagcctcgtt accatgccta tcagcggcac ttcaggctca
    19861 atgggaaggc ctagctctct ttggagcgat tccttgcaga tagcttttcc tgcgatgtcc
    19921 tcaacggaat agtgtgccgg cagataggga tccttcgaag gattccatat ttccgtatcg
    19981 atgccattta gaatgcctac caggtctctg cttcgctgcc gcaacaggct gtcgagtcca
    20041 aaaccaccgc ttggagtttg gatttccata gcgtaagtag gcgaaacggt tgcgatgcac
    20101 tcggcagaga ctatccccgc cttgagaaaa ttgatgttgc cataatattc aaaaccagct
    20161 ccataataat attcccaacc taagccaagc tcggcaaaat gatctctacc atagattccc
    20221 tgataaccca aattatggat ggtcaagaca ccggcggtcc ctgaaaactc ggtattcttc
    20281 tctatagtat taaggtaaac aggaaccggc gctgttggcc aatcatgcgc ctgcatgata
    20341 tctggaatcc atccaaggtt gcggcagacc gcaaaggcag acctggacaa aaccgaaaaa
    20401 cgctcaggat tatctggaaa ttcctggttc gaggaatagc cataaattcc atttcgacca
    20461 tatagacttt cgcattccag aaaataaacc ttaacctggg agtttgggag attggttgta
    20521 taaagaaatg cccgatggcg ccctcgtgca gaatcgatct ccagaagacc atttatcctt
    20581 ctcagaatgt tctttgatat gaagtagtag cgtggaagca gcactcgcac atcgtgaccc
    20641 aacttagaaa gcgcagctgc gagcgaagat actgcatcac cgagtccacc gctttttgca
    20701 aatggtgtca actcgcttga aataaacagt attttcacaa tccgcctccc atctcaatgt
    20761 tctagccatt cattatatga tgcgaggcta ggatgctgca atgcggcttc aaggctgctt
    20821 ttcatctgta gcctcgggca gggcgggttt ggcaaacact ccgatgtaaa cactccgatg
    20881 ctcagatatg gacataattc ctgaagcaat gagccagttt ttgcgcgact ctgtctttag
    20941 gaggagaata tgacggagcg cagaatattc ttatcgcttg gagcagttat ctttattaca
    21001 gctttcttgt tcatagcttg cccttcagga attcctaagg gtaatggata tgtatacata
    21061 cgatgtgtct tgccaggtaa cctgacagaa ggaaacacca ggaaaactca atcaaggctt
    21121 ataggccctg aatccggatg gaatatagca tcctatcgtt tgacattcac gagtgagaat
    21181 ggagaagtca tcaccaaatc tatcggtaag accggcgcgg agctgagcct caaggctggc
    21241 aattggtctc tactagccga aggccttact gctgacgggg ctataatagt caaaaaagaa
    21301 acgcaagtcc tggctcaagc aggaaagaga gtcaacatac agataatgat gcacttagct
    21361 caaggaaaag gctctctgca gctagcatta tctccaaatc agccacttcc gcaggactgg
    21421 aaatactctt tgtcgctcga atacagaggc ctcccaggag atcccacttt tcagggacca
    21481 gctcctttat cgacagatgt tccggctaca cagatggatt atcaggtcaa tgaactggaa
    21541 tcaggaaatt acagccttgc aatacaagtt aaagatactt cttccacctc aatagcgggc
    21601 tgtattacaa cagtgctaat cttgccagaa caaacttcac gaggctcatg tagtatatcc
    21661 cttccggatc catcggccac catttcgctt agcactccaa aattggaatt atcggccgat
    21721 gctgcaattt ctgttgagcg gtatttgaac cgaaataagc ccatctctgt gccttttgta
    21781 attgcacaag gagaggccga tctagatgtc caatggtacc agaatggaac aataatcgag
    21841 gaaactcagg cagaagcaga ccaaagtctg cctggttata agaccttctt ttcgccagac
    21901 aagcaacccg atactcaaat gcgaatatcc atggaagtgc ttcttagcga agggaattct
    21961 ggcatttcgc aggcattctc tcatagttca tcgcttctta caggaccctc atcaagcgaa
    22021 atcgaatgga tacagagtat cgactataag gctgcactaa atccatcgct ccacaacagc
    22081 atctatcctg aaaatgccgg tactggtaat ttgcatgagg caaaatcggt agcaacaagc
    22141 cctactggat tgatagcggt agccgggctc gaccaggcca gtgctctgca tcttttctat
    22201 tctccatatg gcgagcaggc tacattagat ggcaacaacc gccaaatccc ttcttctatc
    22261 ggatggatac gcctatggcg ggataggata tttatccaaa aaagcgaaaa aaacccggat
    22321 atagcaagca tatcaatgga tggatcgatg atagctatcg cagcgactgc cggaaaatgg
    22381 atcagggtct atagacttga aaattctggt tccatctcaa ggaaaatcga tattatcagt
    22441 ggcgagaacg gagccccgac atttgagaat ataagggcaa tgaggtttac aaccgattcc
    22501 acaaggctat tcatcctagc aaattcatca ggaaagttac ttgtattaga tatcgaaaag
    22561 ctctttcgcg gagaaccatg ttgcgaacag gaattctcta taacaggttg gctcaataga
    22621 acatctgaag gctctcagca aaatccttct gagacagagc attcttcatt agggatgcaa
    22681 gatatgattc tactagacga tggctggatt gcagcgtgtt cttcgaccat caagatggta
    22741 attttcatta ggtattcatc gtctgactgc tctttttccg agccgatcat atttacatct
    22801 ggctcaaatt ctgcatctct tggagaccca aaatcgatcg cctttgatga gggcaatgaa
    22861 cgatgctatg tgatcggcgc tgccaggaaa ttgcatttgt tcgaaaagtc agatccgcta
    22921 tctgcttatt ctctatcaat gacctacagt ctttcttctg atttgaatga agccaattcg
    22981 cttgcgcttg taaaaaagaa ctctggcgga gcctatctag tcgcaggagg gaatcatggc
    23041 cttggaatca tatcgctgga tactgatggg cacccaattt gccaaggctc tttgaattct
    23101 caagaagaca atctctatgg aataaaatca ataaacaatg tcacagttct tggagaatcc
    23161 gtaatctcag cgggaggaag cagtggtatt gttgccatgt tcgatattct gggcttataa
    23221 ggcaacacaa ttcccaaaac caccatttat cgcgagagat aaaaagagac ccttagcccc
    23281 aacaaacaat aaaaaagaga aagacttgga tgaaatcttt ctctttttga tattcgtgca
    23341 ttgaaataaa aatactagct gcgataacag agtatgggat gtcgcgctgc agccacctca
    23401 tctagacgag ctacgggcgc aatatgtgga gcatcatgca gaagctccgg atccgttctc
    23461 gcttcttctg cgattttttt catcgcctca gcaaaaagat ctagggtttc cttagactcc
    23521 gtctctgttg gctcgatcat cagggcttcc tgtacgatga gcgggaaata gattgtagga
    23581 ggatggaagc catagtctat aagacgcttc gctatatcca atgtgtggac atcagcggca
    23641 atcgttccct tggcaacgaa ttcatgcatg caatgtcttt tgtaagggac aggatagtca
    23701 ttctctaaaa gcgacttaag gtaattcgca tttagtactg cattctcggc aacctgacga
    23761 agtccatccg caccggtcat caacaagtag cagtatgcac gcaccaggac gccaaagttg
    23821 ccgtggaagg cctttaccct gcctatgctc ttggccggca ttttaagacg atagccggat
    23881 gcttccttga tggcgatagg gccaggcagg tatggcgcca aggtctcatt ggctgctacc
    23941 attcctgcac ccgggccgcc tccaccatgc ggagtagaaa aagtcttgtg tagattgaaa
    24001 tgcataacat cgatgcccga tctgcccggc ttgaaaacgc ccgtaagagc gttcatgttt
    24061 gctccatcgc catatacaaa gccaccagcc tcatgcacaa gtctgcaaat atcctctatg
    24121 ttcctctcaa aaagcccaag agtattcgga ttggtgatca ttattccagc tgtagtatca
    24181 tcgagagcag cgcgaagcgc ttccatatct atactaccat caggccccga gggtatggtc
    24241 tgagttgtaa atccggccat agtgcagctt gcggggtttg tcccgtgagc tgaatctggt
    24301 atgagcatct tttttcgctc cgtctctccc agttctttca aagcggcatg aatcatcagt
    24361 acgcctgaaa gctcaccatg ggcacctgct gcaggttgaa gagaagcagc ggcaaaacca
    24421 cccacttcgc gaagtccctc ctgaagtctc catagaagct caatggctcc ctgtgcatat
    24481 tgctcttctg cgagagggtg aagattcttc catccaggat tcgaagcaac aacctcgttg
    24541 gctttgggat tgtatttcat ggtacacgag ccaagtggat agaactcagt atcgatggaa
    24601 aaattctttt gggagagtct cgtaaaatgt ctaaccaccg taagctcatc gagttccggt
    24661 agatccaatt cagtgcgagc aagctcagga ggcattcctg tgctcggaac atcggacctt
    24721 ggaagagaac aaccaattct tccttcctta gacaattcat agaccaaagg ctcgtcgcgc
    24781 atattaatct cattcatttt gcagcctcct tcaatgcaac agcgagcttt tctatttgag
    24841 cttttgtatt catttctgta acgcaaatca agagctcatt ctttctctca ggataatagc
    24901 gggagagagc caaacctggc acaataccct tcttgttcaa tgcttctacg atcttggcag
    24961 ccgcatctgg tgtttcgacc aggaactccc taaaaaacag tttcggaact acatgatatc
    25021 ctggaataga gtcgataagt tgagcagcat agtgagcttt atcgtaggat agtctagcaa
    25081 ctttctggag cccattcttg ccgagcgtcg ctagatagat gcaggtctgc aacatgctca
    25141 agccttgatt cgaacatata tttgagaccg ccttttctct tctgatatgc tgttctcttg
    25201 tggacaaggt aagcacaaaa ccgcgtttgc cgaatttatc ttttgtctga ccaacgatcc
    25261 tgcccggcat gcgccttatg agtgcatctg tcgtgcccat cattcccagg taaggacccc
    25321 caaagttcaa tgggttacca aggcattgcc cttcggctgt tacgatatct gcgccctgct
    25381 ctccggggct cttgaagatt gcgcacatta agggatcagc ttgcacaatg cagagcgctc
    25441 ctttttcgtg agccttatcg gaagccttgc tgactgggta aaattgtcca gaaaaacccg
    25501 gataggctac gactattgcc gcagtgtctt catctattgc tacctggcca ggatctcctt
    25561 cgtatctgat gaattctata tcgaaagaag tacagtaggt ggaaattact tgagaatact
    25621 ccggatgaag atccttgggc aggaagattc gatttctgtt ttctctggcc ttccaggcca
    25681 tgagcaccgc ttcagccaac gctgtggctc catcgtaatg agaagcattg actacagaca
    25741 ttcctaagag ctttgccgtc atgctctggt attcgaagat cgcctgcaat gtgccctgag
    25801 atacttccgg ctgataggga gtgtaggctg tcaaaaattc gcctctgcct gcgagggcag
    25861 gcacaactgc cggaatatag ctatagtatg cgccagctcc gagaaaccaa tccatatctt
    25921 ttgtctggac attgcgactt gccaaagtct ctagctgatc gatcgcttca atctcagaga
    25981 tagattcagg aagcgccaga tcctctattc gcagatgctc aggaatatca gagaacagat
    26041 cttctatgga agggattccg atcgctttta gcatctcagc cctttctaga tcagtatgcg
    26101 gaacaaagga cacgatgtca tacctcccct atggatgcgc cataggattc cgcgtcgaga
    26161 aggctctcga attctgaaag tttggatgga gccactttta taagccagcc agaaccatag
    26221 cagtctttat ttacaagaga agggtcattt ttcacttctt cgttgacctc caccaccttg
    26281 cccgaaatag gcatgtagag gtcgcttgct gcttttactg actcaattgt tccaaatacg
    26341 actcccttat cgagctcttt tcccactgcg ggaaactcaa taaacacgac atcgcctagt
    26401 tcggcttgag catgatcaga aatccctatt accatgagct cgccttctgg ctttgcatac
    26461 tcatgtgact caaggtacct agcattctta tcgatcgcca tactttcctc ccttaagaga
    26521 aattatatat tgcaagacgc ttagtgcgta ctagagtttt agcgccgata tacaggcaca
    26581 tagaagggac gcttaatgac ttcagccgac cgaggctggc cacggatgat aaccgatagc
    26641 tttgtaccta tcttggcata gtcaggaata acaaaggcat tggccgcata tttctttacc
    26701 gtcgggcaat acatacctgc aacgcagcga ccgataatgt caccatctgg cgtagcgact
    26761 tcatatcctt cccgagcaac gcctccaaag acctcaagac caacaagctt tcgtctgaca
    26821 ccttgttcta taatagaaag cagagcatct tttcctatga attctttttc gaagtcgcaa
    26881 gcccatctga atcccgcctc gatggggttg atatcgacac caatctcatg cccatgaagt
    26941 ggcaatcccg cttcaaatcg cagactgtct cttgccgcaa gtccaattgg tctggtttcg
    27001 ataccataac tttctccgca ttcaagaagg tattcccaaa gctcaatggc cttttctgcg
    27061 ggaaagaaaa gctctccgcc atcttcacca gtatagcctg tacgaccgaa gaggatagga
    27121 atacctctta tggagatatt tgcccagcaa aagcgcggga tcggcgaaac cggagtattg
    27181 gatattttat ccaagagttc aatggctcgg ggaccttgaa cagcgatcat atatgtgtca
    27241 tccgaacgat cgacaacaga gacattcgaa ggaacatgtg atcggaacca ttcaagatct
    27301 accgctcgat tcgatgcatt cacgacgatc caccaggaat tctgcaatct ataaacaaag
    27361 agatcatcaa taatcccgcc cttttcatta agcaggagtg agtatcgagc cattggcgca
    27421 acaagatccg agactcttgc gcttatcata tgagagacaa aactctctgc ggcatcaccc
    27481 gttatttcga actgacccat atgatctata tcaaagagac caaccccccg ccgcgtaata
    27541 agatgttctt cgatagcgcc ggtaggataa ttgatcggca tatcgtagcc agcaaaaggt
    27601 gccatcttag cgccatgcga gacatgccaa tcatgcaatg ccgtggtatt cgtaggctcc
    27661 tcctctatta tgaaatgctt tgtttattct aacatcactc atgcatactg acaacgagct
    27721 tatttagaaa ctagttcgag accagggttt atcgaggaca atgccttcgg aaagaaaagc
    27781 acgggatttc ccctctatta agaattggac atcattgata ttctgaaatt ctgtaagcgt
    27841 atagactatt tgcttaagct gagctaaata cccttcttta ccataacgat tatatgcaaa
    27901 tgcttcgtta aaatccaaaa tagcagtacc gccttttata ttgacacttc gaagttttgt
    27961 gtctgcagga attaacgata gaagattcgc ccgcaattct gattccgttg gccctttcaa
    28021 aagcgactca agagtatcct gcaggggact gtccgatatt gccagtgtcc gtttcacttt
    28081 ttggctggaa atcgagccat catctcctat gtacacaaaa tatagaacgg ctgtcctcgt
    28141 cttttgttct tctttggaca gcttggagat agatgctccc ggagaagagg ctattggctc
    28201 tgactctgat agcggagcag aaggcaactc gacttctttt ggcgctttgg tggtcgtggt
    28261 cgtttctttg ggtagtattg gactttgaat ctgcggttct tccgcaggtg gcgctgcaga
    28321 tggtagtgtc ggtgcaggcg caggttcttc cggattagtt tcctgcggtt tctgttgagg
    28381 ttcagaatct tgcttggata cagcatggtt gagagcatct ataaagcctg ttttctcaac
    28441 agccgtacga atatcctcaa atttgaccag gaatagaatt agaagaatga gaaaggctcc
    28501 aagccagatt agacaacccg tttttgcttg gttacgcttt ttctttcgca ccgccatatt
    28561 gtattgatcc tacttttagc gagccaaagg gtcaaggaca agactggctg cctttttggg
    28621 ttttggttga tcgggcagaa ttgtgaaaaa atcggctctc ctggactcag catccaccaa
    28681 aacaacctgc cataatgctg ggttgtgtat ctgcacaagc tgcccctctg ccctaagtgc
    28741 aaagtgtcct gatacaggcc tatggccgga aaaccaaacc gaatgccttg ctctatcttc
    28801 agatcctaaa attgatttga gagatttcct tacggagcct tcctcggcct catcatttga
    28861 agtccatatg agcgcttgaa cgattcttga atttgaacga tatgaaatga tggaatctct
    28921 agtgatttcc tgggctggct cagcatggct tgcacagaaa ttcaaattct cagccgcaat
    28981 tgccagggag agttcatatt ctcgcaactt atgcattata aatgggccat atcgaaatga
    29041 gagccagata gctcccattt tgccctcatc tgcgtattta taaaatggaa aatcaccatg
    29101 atctccagca ttagtcatat tatcgtgatt cccctttaag caaaataccg aatttggaaa
    29161 accacactgt agttcgatca ctttcagcaa ggaagtcaga gagaggccca tctcttcatc
    29221 catttcgggc gaaagaagtg ctcttacatc acgagcaatc aaagccttat cgtaagaact
    29281 aatccagcgt ctggcagcct cttcgccctc tgcatgcgga atatctccta gaaagagaag
    29341 tgttgcatta ttcttcgaaa gcacagaaag caatggttcg ctaaggcctg gaaactcggt
    29401 tagcagcagg gcatgaagaa agctcggcct ggcatgcaga tcagggacaa gtatgcaggg
    29461 cttatcaggc ggaagaacaa tcaagcctcc cggcagagaa ttggctggat cggatggtct
    29521 caattctgca gaaacatgaa gatttgcagc aatcccagca tctatcgctg cctcaagttc
    29581 ttcggcagca gggggttccg aactccgaaa ctcgcttctt atggtcatct cgatatcgcc
    29641 gttcttatgc tccagcccct tttctttccc tcaaagcttg gatcttcttt atccattcct
    29701 tggcaattct atcatcagga tccaattcga ttgaagtcct aaagaagcct tctgcttcat
    29761 ctatatgccc ttggcgaaag gcaagagcac ctagatttac aatgattttc tcattttctg
    29821 gctctattct cagggctctt tcaaggtgag acctagcagc ctcgagatca cctgcctcta
    29881 tctcacagat agatagctca ttgtagacat cagccccatc ggctcctttt tcaagagcag
    29941 tatgaaatgc ttgggcacct tccgaccata gccccaacct gcgatttgcc catcctacca
    30001 gaaaccaacc attccagaca tcggggtact tttctacaaa ctgcattgct ttttcaagcc
    30061 ccttttcctc ttgccccatt tgaatgaaat cgtatgcttc tttgaaaagc gtatcgagat
    30121 agcccatgcc gccaagtttt tcaagaatta acttggctct gcgagtacgt tgttcgtcat
    30181 cattcatccc gatataactt gtcaataggg aaacagcctt gtcatagttg tgctttctca
    30241 agaaaaagaa agccgcatga taatatgcgg gagcaaacac atccttgaat ccgagaagat
    30301 actggtagta tttatatgcc agatcgtctt ctttttcggc ttcatcttca tttccagaat
    30361 caaaaagccg cttagcgtgt tcttcatgca tgagcgcaag attgatataa ggctcaggcc
    30421 gctccggctc aaggccgctt gccgcgaaga aaacctcctc agcaacgatc caatcttttt
    30481 cttcggcttt ctgaattccc gccactatca actcctcgaa aagctctgga cgggacatct
    30541 ttatatattt tcgatattta ttggcattct cgttctctga gtcccaagca agcactctta
    30601 acatacctgc caaaaggctt tcgatagtaa tagatgaagg atcaagtttg accgcgggag
    30661 cctgtagctg gacaggatat ggaatactgg catcgattcc tgatggcaag cgcgattgtg
    30721 catcagcacc ctcgaagcgg atatacaaga tgctcgaaag agcttgcttc ttgcgctctt
    30781 cgaaggcttc gggcaaaagt tgtctgttat cttgatttgc tcgtcctttt ttttgatttc
    30841 ctcggctctt gtctcgcgat ggcggtttca tgcctggttc tctcctcgat ttatctatca
    30901 ttatcaatcc ttccagggga aaatcatccc tttcaacgtt ctctttccaa ccctctcttt
    30961 ctcctcttga agaagatcat cccaggggat gtgatgtctg tctgccccag gattgtcatt
    31021 tagatatcca tattttctca tcctaagcct cactgctact gcggacgcaa gcgagcttcc
    31081 ggcaggcccg ggcgctaaca gtacaacaaa gatcgatata acgaaggtca gaattctcca
    31141 gaaaaacaag aatatcgaaa aacctggatt atcgataaaa agataaagcg ccatcttaaa
    31201 agcctggcgg ggagtacccc cttctcgtgc aaagactgcc ggcacatact gcaaaatcag
    31261 cgtggccaga atcatcatcc agaatagaag gccccctata aagatgccca agactgcctt
    31321 ttggccaagg taaaagggaa tagctatcat tatagcaacc acaattagga tattgacccc
    31381 gccaagcaat gaaccaagct tcaaagatct tctgatggca gactttatgt ccgcaaaatg
    31441 aacatttttc ccatccgcta tattatacat ggcattggtc accactacat cccatattga
    31501 gagtagcaga atgccaaacc cagccataat aacagaaagc caaataggaa aagccagcgc
    31561 aattgggact acaagaaata aaagaagaat accgagaaaa atcccattca aggctgccaa
    31621 cagatataga ttgtcccagc catcgaaaaa tgctttcttg ataaaaaatc caatcatatt
    31681 atttctatac tccatgcttg acctttggac aagtagatgc ataatgtatg cgc
//
LOCUS       DAOOWY010000011        29727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1567_length_29727_cov_9.884538, whole genome shotgun sequence.
ACCESSION   DAOOWY010000011 DAOOWY010000000
VERSION     DAOOWY010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 29727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29727
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1567_length_29727_cov_9.884538"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /gene="ispE"
                     /locus_tag="PK105_01810"
     CDS             complement(<1..995)
                     /gene="ispE"
                     /locus_tag="PK105_01810"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOU60472.1"
                     /translation="MPTLEIKVFAKINIGLKVFQSRTSGYHDIESIFQNVSLADTIWI
                     STSNKSNKARVTIEGDFGCPIESSSVYKAAIEFDRATDGIIRRNGVHVQVGKEIPAQA
                     GLGAASADAAATLCGLNELFGNRFSKKELARISIRIGSDVPFFLFGEGAAIVRGRGER
                     ISLIKPRTDFGIVILLPAWASSTKDAYRLLDVMRRNGLSRIHSSTEVCEGESTYRVCH
                     SDGDLKKWYDGPIAYWHLGNDFQQVMEEQHREYEVWSQLLIDNGAVCTSLTGSGSAMY
                     GVFESMREADAAAEECRNLIKRVEKSELCNIVSIFSVVPLARSMIVSYIQACNVDT"
     gene            1188..1523
                     /locus_tag="PK105_01815"
     CDS             1188..1523
                     /locus_tag="PK105_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60473.1"
                     /translation="MNHKIKMTADTQEEFELITLTNGDSADTIYSLRPTVRFDGAYDD
                     EFDYDSDDDEFDDEEDLEDEDDFEEDEEFEDEEDIDEDFDEDEDIGEDEEFEDEDFED
                     EDFDYDTDE"
     gene            complement(1618..1980)
                     /gene="rsfS"
                     /locus_tag="PK105_01820"
     CDS             complement(1618..1980)
                     /gene="rsfS"
                     /locus_tag="PK105_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOU60474.1"
                     /translation="MDSKAFAYQSAEILAQHKALNVLVMDVHEIAGWADYFVLATSTS
                     STHMRGLQKHIEDFLKVEKIDLLNKPEVTDDQCWLLMDAQDVIIHIMSAEARDFYDLE
                     SLWFNAPRFAVAFRGNGI"
     gene            complement(1970..3160)
                     /locus_tag="PK105_01825"
     CDS             complement(1970..3160)
                     /locus_tag="PK105_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOU60475.1"
                     /translation="MSDSKSKILKRSTLPLLVILIVAAVGIFFVVKLTEKGTVESIIK
                     KDQPMAMLFIFESNKKPVSNQLLIWYPSRRKAAIMDIPSTMGIILKSANKMSSIDTVY
                     DSRNPSKFVKEISDYLKYPIDGWFVYDEASLCQTIDLLEGIQMFIPETVMESDSLKGV
                     SLPGGAVVLDGDKTNQYLLYALATDSYSEEISRKQRLLINMLGRLADESASLNKKSNL
                     RLIASKPKSNFSLATRLSIFEHLAQIDHDMIITQFISGSFRSFEGRRLLFPYYDGELA
                     RDVVSQTAKALGIEEDGASQKTIVTIDILNGCGEKGLANTASILFESYGYKVVSVGNA
                     PSFDYAKTVMYDNDGDKAALQQVANIINCKNFGDASVLPGLQKANITIILGKDFNGRY
                     CIGQ"
     gene            complement(3157..3771)
                     /gene="yqeK"
                     /locus_tag="PK105_01830"
     CDS             complement(3157..3771)
                     /gene="yqeK"
                     /locus_tag="PK105_01830"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOU60476.1"
                     /translation="MDYIEIPEIASIIEKEIACNLSPKRYAHSRSTSETAVKLAERFG
                     EDRWAAHLAGLSHDMCRGLSFEEQNNLVNKHRACIAFLSERPSLAAILSDREYREKMI
                     HGPAAACSLCYRFQVREESILEAVALHSIADDAMSPLAKIVYIADKLEPLRNRSADAD
                     EKMQTLDLDSLFAYTLASVVRWFSESGKPLCPYTAEIYIRMTKS"
     gene            complement(3749..4327)
                     /gene="nadD"
                     /locus_tag="PK105_01835"
     CDS             complement(3749..4327)
                     /gene="nadD"
                     /locus_tag="PK105_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOU60477.1"
                     /translation="MRIAIIGGSFNPPHIGHMILIEEILATGRYQRVLLIPAKTAPHK
                     VPQMDPGPEMRLALLEASIEGWPKVAIEDCELKRTGLSYTSDTLEELWDRKLFDEKPG
                     LVIGDDLAPDFLATWKRAEKILELADIIVAHRLYVENIPLPYPHDYLDNLVIPISSTL
                     VRGRIHGGGAWRSLVMPSVRTIIENYGLYRNT"
     gene            complement(4327..5433)
                     /gene="obgE"
                     /locus_tag="PK105_01840"
     CDS             complement(4327..5433)
                     /gene="obgE"
                     /locus_tag="PK105_01840"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOU60478.1"
                     /translation="MIKFADEAIISVASGKGGDGCVAFRRERFIPLGGPAGGDGGRGG
                     DVVFVVKRNLRTLAYLRYRQVFKAENGQPGMGKNMHGRDGKDVIIEVPPGTILRDAQT
                     NEIIRDFVYRARGKKLGALEDEEPWIFLKGGKGGWGNTHFKSSVNQAPRFAQPGQPGQ
                     ERKLKVELSLIADIGLVGFPNAGKSSLLEYFTNARPKIAPYPFTTKIPNLGVLTVNER
                     DIILADIPGIIEGAHEGAGLGLRFLKHIARTACLAFLVDLSDEHWKAAFPTLLRELEL
                     FSPTLVKKPRILLGTKMDLSESQANFIDFKASFPEESIYGISVFTGQGLDEVKQQLFE
                     KVLAYEAAENYESQKENAKERIFDEIDFSDEGFI"
     gene            complement(5517..5762)
                     /gene="rpmA"
                     /locus_tag="PK105_01845"
     CDS             complement(5517..5762)
                     /gene="rpmA"
                     /locus_tag="PK105_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOU60479.1"
                     /translation="MPHKGVNGRDSNPQCLGVKAYASERISAGSIIVRQRGSKINPGK
                     NVGKGKDDTLFALIDGTVRFHEYKGKKFVDVEPAIQA"
     gene            complement(5792..6103)
                     /gene="rplU"
                     /locus_tag="PK105_01850"
     CDS             complement(5792..6103)
                     /gene="rplU"
                     /locus_tag="PK105_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOU60480.1"
                     /translation="MYAIVEINGKQYHAEQGKDLVVDRFDAMPGQSIVFEKVLLLSGD
                     ETKVGTPYVNDAAIKATVREEIKGDKIIVFKYKPKKGYRKTRGHRQPYTVLTVNEIVG
                     A"
     gene            complement(6223..7995)
                     /gene="argS"
                     /locus_tag="PK105_01855"
     CDS             complement(6223..7995)
                     /gene="argS"
                     /locus_tag="PK105_01855"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOU60481.1"
                     /translation="MEYFVDQWKSIIANCLNELALQKELKDVSFSSQDIIAEKPPRPK
                     LGDIGFPMFSYAKQFRMSPDRIAAEIASLCNMTIDIEKLILPGTINATGSYLNIYLDK
                     EYVTNYVLENAEKEGWGSSPLFNGQRIMIEFSCPNTNKPLHLGHLRNNVLGESLSRIM
                     KAAGAEVKKVNLINDRGIHICKSMLAYLAYGQGRTPEDEGLKSDHFVGKYYVLFNRLK
                     EEDPFAEEKAQELLQKWEAGDPDVIALWEKMNKWAVEGIMDTYRRQRVSFDEFHFEHE
                     TYKLGKQEVLEGLARGIFYRAEDGAIQVDLEDVGLGKKVLLRKDGTSIYITQDIGTAI
                     MRYNRWPFHELVYVVASEQQYHFKVLFEVLRRLGYSWAENLYHLSYGLVNLPSGRMKT
                     REGTVVDADDLIDELTELAANEIADKGREEAVGDIEDVAEQVALGALHYFLLQVSPGK
                     DMLYNPEQSLSFTGDTGPYIQYMGARASSILRKHKNGEGNAGQGKIDVSLIVSDSDWS
                     LMRLLMDFPYTIEQSAKNKDPSILAAYAHQLASEFSAWYRDNPVLKNDNPDISASRLA
                     LVTAIKEILRRASNLLCIPFLDAM"
     gene            complement(8009..8320)
                     /locus_tag="PK105_01860"
     CDS             complement(8009..8320)
                     /locus_tag="PK105_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOU60482.1"
                     /translation="MRTPSARLLEHLDILKPALHEEKEGIVLDVYVIPRAPYDSVEGL
                     RDGRILLRVKAAPEKGKANEAVLDLIADYFDIASSNLRILRGHSSKRKSILLQSFREN
                     R"
     gene            complement(8331..10265)
                     /gene="ligA"
                     /locus_tag="PK105_01865"
     CDS             complement(8331..10265)
                     /gene="ligA"
                     /locus_tag="PK105_01865"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOU60483.1"
                     /translation="MPDDLRISEDPDIRQKRIRELEYLIRHHQELYYNGEPEISDEEF
                     DALWDELASIDPANPIIASIGADRSDRWPKYRHRMIMGSLSKASDPESFLAWAAKVRY
                     PLYLVQYKLDGASIELQYDEGGFARAVTRGDGVIGDDITPNAVRMNGVPNRLPEPFTG
                     SVRGEVLMSKRVHASKYSDKANCRNAANGLMKRKDGQGVEDLDIVCYDAVGRLPTGEA
                     FSTEREKIAWLKRMGFNVVPTIECKSPEEIIVYRAKIMELRPTLEYDIDGLVVKGDRI
                     DFEDMNKMRPELQIAFKFSPEEAVTTLIAVEWSESGTTVTPIGIVEPVRLAGTTVQRA
                     NLANPGIIRSMDLRIGSKVVITKRGEIIPKIESLVENPKDAIPIPIPSHCSLCGAGLI
                     DEDTRLYCPNPECPGKAYHRLEKWLSVIDIKDIGSTLLKRLFEAKRVVRIPDLYSLTI
                     DDLISFERIGRKSAQKILRNIRERNEVSLSQFIAGFDIEGIGLLMAEKLIEAGYDTLD
                     KILAASPADFEKIAGFAEISAKTLYEGLRLVEEDMRKLVDSGYVRIRPPLPKTQRTAN
                     PVSGNSFCFTGELRSMKRSEAEKLVREKGGIIKSSVTKDLDYLVTNDPSSGSEKNRKA
                     KELSIKIIDEEQFLEMAGLK"
     gene            10372..10926
                     /locus_tag="PK105_01870"
     CDS             10372..10926
                     /locus_tag="PK105_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOU60484.1"
                     /translation="MRFYAIQVITCKEEEWLERIQHQVPDIKFHKIMKKMYIRKKGKA
                     RLDNSPVFPGYVFFEYDGDSLPLEIVYRLRHSKSFIRFLPNNEGPQPLNNRDSEIIRH
                     FISFGSLIPPSLVRFDENQRIKVIEGPLQGIEGFIIKVNRRKRRAKVRLDIADSIILL
                     DLAFEVMEAETAKAAPLGVYHDKR"
     gene            10913..12793
                     /locus_tag="PK105_01875"
     CDS             10913..12793
                     /locus_tag="PK105_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU60485.1"
                     /translation="MTRDEAVPFEQLLAQDMLYAFAIRAFSNIGNIQNINVPPLPEGY
                     TLITADRLPFKNELRIEGMSLPLLAAKKVSYIGEVVGLIVGPDPLLTEELALNTIVDC
                     EAQESRLDWQVFSSSDISARIDYSRGKEKSSSESLKASEAEDSLELASYLRLEPHEFS
                     FHSGLGALAEWENGRLKLSCPALAPELIHSCLAELTGLSTEDIEILQSKMYDNSGIYF
                     WYPSLLAARSAAAARVLGRPIKLLISIQREKRFLPGIHGISLHMKTRWSTQKHCLMDV
                     ACRFAIPVGAYSVLDELILEKTVRLVADAMPNIPLTIIGFTVKTNMIPMGALDCISSS
                     AIFSMLETHIARAARKMDVELLELLWMTLSGIDSSSIMNKMPPKEESSTEKILRPLLE
                     HTDFHRKYAAYEQIHKRSHGAKETRIRAISLSIAEQSHIDSSCISTMADLAKIRNNWI
                     SYKRYGEGLPIKIQIFSSMKQSDVSDSQSKSIRHLSQGAAILEVDYDSIRSIFHSLRL
                     DISIYAGKILCPSAAKGAIRSASIEALLACLSRSIYQDRNIDDIYKWIFSEGRINIEL
                     MKDEKANFERSIGDLAYTLVLSCFLEVMQQLNSSERLILPLKPTPTAMYSSDLLEKDD
                     EH"
     gene            12783..13262
                     /locus_tag="PK105_01880"
     CDS             12783..13262
                     /locus_tag="PK105_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60486.1"
                     /translation="MNIEFFLNGNLQRIAITSVQRAFDVLVDQCGIKSLSEICPDSEC
                     SNCLILYDGRLVHSYILPAFLLRERHIETIENFTKSKEYGILSAEFDKEGLLSCAYCN
                     NSLMLVAIHLIQRSLSPSWHEISDILASLHCSHIDPRAFADALSKVIQQLPIARKTP"
     gene            13259..14095
                     /locus_tag="PK105_01885"
     CDS             13259..14095
                     /locus_tag="PK105_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU60487.1"
                     /translation="MNLSEIYYPRSIQELIAIMARQKNLQIVAGATSFGYIQDTRYLF
                     FEPSIASIRNIPELKTIHKTERMISFGAACALNDLESVYPFEKKPAREVLRATANSTV
                     RNIATIGGHLMYQKRFLALWPLLACLDAELEFKGPSGTSIKNIWYLTNEEGLPSLESR
                     RVLTRIRIPLQSIDRLFIRRTGAEIFPNGDGAYLVAASTIERNSISYFRLVIAGQRAF
                     RDYDAEQRITSSPYPLTERVFHSVLKMYSESLHRTKFWNMELFLPLISQALEDLQRSS
                     VL"
     gene            14119..14925
                     /locus_tag="PK105_01890"
     CDS             14119..14925
                     /locus_tag="PK105_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU60488.1"
                     /translation="MSENDKNRGTLYLLPVPLLPYSPEDWKPCQLMNMIAPNVIAIMK
                     SVPYFVVESEKTANRLLSRLLSPEQFSEVGFFILNEHSKEEDLKAPLSALKSGYDCAI
                     LSEAGLPCIADPGAALVSLAHEEQLRIVPIGGDSSLLLALAASGLNGQRFCFLGYMPI
                     RSEELQKELVLEGQAAMHDKAARIFIETPYRNERTLRACIEALPENLYLCVACNLGTN
                     QPLIRSMPIASWRNKAIIIPEKPAVFCFGIPAKLNKKQLQSASRERRSLR"
     gene            complement(14889..18284)
                     /gene="mfd"
                     /locus_tag="PK105_01895"
     CDS             complement(14889..18284)
                     /gene="mfd"
                     /locus_tag="PK105_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOU60489.1"
                     /translation="MKKDNLQALFEKISRNPALGEIQKLIEQNSFPLHIQNSENSLLA
                     LSFATLVKRTKRRFLVVLPSDHETEEFSQDLALTGVDLLSFPSWFGAPYRAIPLYSRM
                     FTERASALARLAEGDFEIATVSARTLAIPVPPPEYIKNHILPLKIGADFEPTLISSKL
                     AELGYLRVPSVSLPGEFAVRGEVLDIYMPGYEYAVRIHFDYDRIERIIRFDPETQTGR
                     EKLTQVHVRPLKELVWDKTRIYQLQNNATDFMRNDPRFKEIIEILDSEKQMQGEELWF
                     PLAFDKMYNLVDYLDNRAMLVLVGSERIYAISETLRKEYASMYRIALREVLVPPPNKI
                     HISPDGLFAAFSSVLFCHALKGVDGSAERISLEAEPPHSYFGNIRLFKEELSGFKKEN
                     YKTWIIASTELQAGRISSLIQDDSIVIAKGPISAGFTIPSLKLRVLAEHELFGRRKHV
                     PRSIAKTKSAVIESFIDLTPGDYVVHNNYGIGKFAGIERLIVLGLERDYICIEYAGNE
                     KIFVPIEQANLVQRYIGNEGQAPHLDSVGSKVWENRKKRVSKSVEELAERLIRIYARR
                     KNARGFSFPSDNEWQIQFEAAFPYEETEDQLKCIEEVKRDMESSKPMDRLVCGDVGFG
                     KTEIAMRACFKAILAGKQVAFLAPTTILAEQHFENFLERAGDFPIKVALLSRLVDRKT
                     KNAVIKGLADGSIDMVIGTHRILQKDVRFKDLGLLVVDEEQRFGVKDKERLKEMKVGI
                     DCLTLTATPIPRTLHMSLLKIRDMSVLQTAPLERQPIQTFVEEFSPELVARAIRNEIA
                     RGGQIFYLHNRIETLPEVEYFIRQLVPEAIVESAHGKLNGQDLESIMHRFIHGAFHVL
                     VSTTIIENGIDIPNVNTIIIDRSDNYGISQLYQLKGRVGRSDRQAYAYLLYPDKRALS
                     EIAMKRLQIISDFTELGSGFKIAMKDLEVRGAGNLLGREQSGNIYAVGFDLYLKLLDD
                     AVSRLSGIQNEEEEPYLELEYSGYIPDSYISAPMIKMEIYKRIASVWTQGEIDSLHEE
                     LEERFGPLPDEALSLLSLAEMRVLCRKLSIGRLQERAGIVTVEFSKVSKISAEKILRL
                     IRESAGTIRLSTEKPNAIRIQTKAIGLKEKSAYLKERLSLLAD"
     gene            18365..18766
                     /locus_tag="PK105_01900"
     CDS             18365..18766
                     /locus_tag="PK105_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60490.1"
                     /translation="MLVLVLIEQTSIADLVLRQLRQKSIASIAYRDPLRLLDHLAELN
                     PEAVIIRGKDFPLHQQLLAALIRYYVPLNHCKMIVLGRGIPAFPPCACIAEEQFLKDP
                     SLLTNDLSKTLRIDSRTGSRLVAKAQRTMKL"
     gene            18899..19228
                     /locus_tag="PK105_01905"
     CDS             18899..19228
                     /locus_tag="PK105_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU60491.1"
                     /translation="MNQTGKPCHEEKLRPYAQKFKAIGHPIRLKILCLIAGQEVPCVG
                     DIWRCLDQPQPVVSQHLAILKHYGIVTSEVKKTRRIYSICDPFIRNIIDAMICQINTE
                     PNSTSEK"
     gene            complement(19299..20384)
                     /gene="ispG"
                     /locus_tag="PK105_01910"
                     /gene_synonym="gcpE"
     CDS             complement(19299..20384)
                     /gene="ispG"
                     /locus_tag="PK105_01910"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOU60492.1"
                     /translation="MKETLTVKVGDVLLGGEWPVRIQTMWKNPLPIFPSAEEAQLLPV
                     LEQLSCLKNLGCDIIRFAVPEISTAESLGILAGLSPMPIVADIHFDWRIALRCMDFPI
                     AKIRINPGNIGAEWKVREVATKAKDKAIPIRIGINAGSLPRDLAREKDVIQAAIKAAD
                     REIELLESMSFHDIVVSLKMNNPDEVVRANEEFSLHYPYPLHLGVTEAGPLVAGIVRN
                     TAALVPLLKKGIGATIRVSLSDAMEQEVITGKEILACAGKGKHGVRIISCPRCGRASF
                     DTHAFVSRWMNRLYSINADISIAVMGCIVNGPGEARQADLGITGAGNSVLIFKHGEIL
                     RRVEKEQADITFEEELEALLKDEIEFN"
     gene            complement(20427..20849)
                     /locus_tag="PK105_01915"
     CDS             complement(20427..20849)
                     /locus_tag="PK105_01915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525271.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOU60493.1"
                     /translation="MNIALIGAACVLGFGAIGSGIGAGIAGMAAIGSWKRSYLNNKAA
                     SFLLVAFAGAPLTQTIYSFILMSRIINSTKDPLLLLASGVTAGIAEAVSAIAQGKAAA
                     AGCDAFGETGKGFANYIIVVGLCETVALFVLAFTFSAI"
     gene            complement(20876..22753)
                     /locus_tag="PK105_01920"
     CDS             complement(20876..22753)
                     /locus_tag="PK105_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60494.1"
                     /translation="MKKFYLIVLQRDRENLPERLRKLGVAHVEELQGIGDKYQNLERE
                     RMEVESVHNFLQNYIDKKSKPGKADSSKSLKYEECKDVREIINEISAAKQKLNLLEDQ
                     VIDLKREIDRVASWGEVSMTMLERVVEGAGFSLRFFDFPAKELIALPKETEYIRISAP
                     KGRCRISLIVREGDQIPDLPPSFLEFLPPEIPLSQMRQEIALIENQKQEIIAKLRKHS
                     ANLSLIKKYLHMLDYNIILERLRSGMPQHESFAYMKGYVPVRDCEKFKKTAAKYGWAI
                     AFDDPVEEDQPPTLIENSPAVRIIQPVFEFLGTVPNYWEYDISSWFLIFFSIFFAMIF
                     GDASYGAIIVLATIASIYAGKRKGKPIVDAQKLFLLLGSATIIWGALTATWFGINFDN
                     LPKLLQNISLPLINGQSPDSENNVKVICFLIGLIQLSIAHIKNFKRDFPDLKCMSQAG
                     SLLLLLGMFNAALNLVIDATRYPIENWAIGCIAIGFFLVFIFGNWNGKLGSSLIESLK
                     NIIPTFLGTVSVFADIVSYIRLWAVGLAGLAFSQTVNGMASGMLGGPSGFLIGFVLKL
                     IIAAVILVAAHALNFMLTVLSVIVHGIRLNMLEFSGHLGMEWSGYKYDPLKEDENLSA
                     S"
     gene            complement(22762..23376)
                     /locus_tag="PK105_01925"
     CDS             complement(22762..23376)
                     /locus_tag="PK105_01925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOU60495.1"
                     /translation="MGKIKLTKNELKKKRDELKMYERYLPTLQLKKQQLQVEVRSVQT
                     RMEELQALKKTLEQDASPWMEVFAEQGAARTQEGEPLLKVAFISTDTGNIAGVAIPLF
                     LGVEFEHKGYDLYSTSLWVDAALILMERLVRTQLELEVVSKQKDLLSQELRITTQRVN
                     LFEKVKIPETIADIRKIRIYLGDQETAQVVRGKIAKRKVEGAER"
     gene            complement(23410..24708)
                     /locus_tag="PK105_01930"
     CDS             complement(23410..24708)
                     /locus_tag="PK105_01930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOU60496.1"
                     /translation="MNKIYSRIESIVGNVIAVKAQDIAYGELAQVNTRYGASLAETIR
                     LDSEMVYLQVFSGSRGISTGDEVRFLGHPMQVSFSDNLMGRIFDGAGEPRDGGPALTE
                     NLITIAGPAVNPYQRIIPREMIRTGIPMIDVFNTLVKSQKLPIFSVSGEPYNPLLARI
                     AMQAEVDLIILGGMGLKYDDYLFFKDTLEQGGSLSKTIMFVHTAADPIVECLMVPDIS
                     LAIAERFALKGKNVLVLLSDMTNFADALKEIAITQEQVPSNRGYPGDLYSQLAARYEK
                     AVDFSDAGSITILAVTTMPGDDVTHPIPDNTGYITEGQFYLRDGHIEPFGSLSRLKQL
                     VNAKTREDHRSIMDAMIKLFAAYRDTLEKKSMGFQMSAWDRKLLKYGELFEQRIMDLS
                     VNIPLEKALDLCWETLAECFEPRETGLRSDLIQKFWIGKK"
     gene            complement(24712..26475)
                     /locus_tag="PK105_01935"
     CDS             complement(24712..26475)
                     /locus_tag="PK105_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOU60497.1"
                     /translation="MTEGFVTAVNGNMVSIQTEGIVTMNEVAYILVGERRLKSEVIRI
                     RGTSVQAQVYEITKGIKVGNRVEFTNELLSVELGPGLLGQIYDGLQNPLPEIANKIGY
                     FLEPGLYLKALPRDIKWHFTPILKKGDILKRGMGIGWVPERYFRHIIMMPFDLYGEWK
                     VADIVAEGDYTVDEAIAEVVDLKGNKRHIQMSFSWPVKRAVDCYSERLKPSEPLVTKT
                     RIIDTFFPVAKGGTYCIPGPFGAGKTVLQQVTSRHAEVDIVLIAACGERAGEVVETLK
                     EFPQIIDPKTGRSLMERTVIICNTSSMPVAAREASVYTAVTIAEYYRQMGLDVLLLAD
                     STSRWAQAMREMSGRLEEIPGEEAFPAYLESVIAAFYERAGIVRLYNGKEGSLTIGGT
                     VSPAGGNFEEPVTQATLKVVGAFHGLSRERSDARKYPSIHPLDSWTKYDGIMPKSAVR
                     YAHSFLERGAEISAMMKVVGEEGTSLEDYVIYLKSEFLDAVYLQQNSFDPVDAAVSPA
                     RQKHVFKLVLKILAAGIEFSDKEEARNFFYELRQTMLDLNGTPQDSDMFSALYNRVEE
                     ALVDRKPEFDDRGLKLISTLE"
     gene            complement(26472..27056)
                     /locus_tag="PK105_01940"
     CDS             complement(26472..27056)
                     /locus_tag="PK105_01940"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOU60498.1"
                     /translation="MKHYWYFASTLTSFPFGSPPPISMQAFDELCSRMMDKSDLEILL
                     YVEEARNGRFSDSMMKSPFLMSYYEFERGLRNALVVYRTRATKQDAGQWTRPGDMNPD
                     SLKAAQAAQSASDPLQAELALERERWNATERLSALSAFELDYILAYKMKLLIASRIAS
                     FDRNRGMQGFNVLYQDIVNAAAEAASAFDTGVAT"
     gene            complement(27058..27675)
                     /locus_tag="PK105_01945"
     CDS             complement(27058..27675)
                     /locus_tag="PK105_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60499.1"
                     /translation="MEIQLQELLERIHSEGIENAKQQAEQIIQKAQSEAAEIIARAKR
                     DAEEMQAEALRRIDAMEAASRESLLQASRDTMIALRQSVQRFLDSAISADIKKAFDEE
                     MAAQVIPEVLKILSSGQSSDFEVLLPPEQLEKIDSSLSARLAKELSRGVTFKPYDGID
                     AGFRIALSGSSVQYDFSADSIAQMLSVRVNRLLSQYLKESAGSLD"
     gene            28028..28099
                     /locus_tag="PK105_01950"
     tRNA            28028..28099
                     /locus_tag="PK105_01950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28061..28063,aa:Gly,seq:tcc)
     gene            28291..29532
                     /locus_tag="PK105_01955"
     CDS             28291..29532
                     /locus_tag="PK105_01955"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOU60500.1"
                     /translation="MNSGITIMKPKVKTPIVELDGDEMTRVIWSMIKEKLILPYIDVH
                     LEYYDLGIANRDVTNDRVTMDAARAIAKYGVGVKCATITPDSERVKEYRLKKAWPSPN
                     ATIRAYLDGTVFRKPIIAKNIAPAVRSWQKPIIIGRHAYGDIYKAVDFVVPGPGTVSL
                     VYQPAQLGMPISFKVHEFEGPGVAMGIHNTDASIRSFARACIEYALSEKTDIWFAAKD
                     TISKNYHGHFKELFGEEISARSADLEAAGIHYRYMLIDDAVAQLMKNEGGILWACMNY
                     DGDVFSDMVASGFGSLGMMSSVLVSPSGAFEYEAAHGTVRRHYYEYLKGNPTSTNPIA
                     SIFAWTGALRKRAELDKTPEITIFAQQLEDTAIELIESGVVTKDLINLISPPAKSFET
                     TESFLDKIDAGLARKRTALQA"
BASE COUNT         8103 a         7124 c         6167 g         8333 t
ORIGIN      
        1 gtatctacgt tgcaagcttg aatataactt acaatcatgc ttcgcgcaag cggtactact
       61 gagaaaatgc taacaatatt gcacaattcc gatttctcga ctctcttgat cagatttctg
      121 cattcttcgg cagccgcatc ggcctctctc atggattcga aaactccata catagctgag
      181 ccagagccag taaggcttgt acagaccgca ccattatcga taagtaactg cgaccacact
      241 tcatattctc tgtgctgttc ttccattacc tgctgaaaat cgtttcccag atgccaatag
      301 gcaatgggac catcatacca tttcttcaaa tcgccgtccg aatggcaaac acgataagtt
      361 gattcgccct cacaaacctc ggtcgaggaa tgaatccgag aaagaccatt tcggcgcata
      421 acatccaaaa gcctataggc atccttagta gaagaagccc atgcaggcag caaaatgact
      481 attccaaaat cagtccttgg ttttatgaga cttatccgct cgcctcgccc cctaacgatg
      541 gctgcaccct ccccaaacaa aaagaaggga acatcgcttc ctattcttat tgaaatgcga
      601 gcgagttctt tttttgagaa tcgattgcca aagagttcgt ttaagccgca aagcgtggct
      661 gcggcatcag cgctggctgc acccaagcct gcctgtgcag gaatttcttt tccaacctgg
      721 acatgtacac cgtttcgtct tatgattcca tcggtagctc tatcgaattc aatcgctgct
      781 ttatatacac ttgatgattc tatagggcat ccaaagtcac cttctatggt tactcttgct
      841 ttattgctct tattgctggt tgatatccaa atagtatcgg ctagactgac attctgaaat
      901 atgctttcta tgtcatggta tccggaggtt ctgctctgaa ataccttaag tccgatattg
      961 atctttgcaa agaccttaat ttcgagtgta ggcatctttc atggtattat atatgacaat
     1021 tttttctgtt aaaagccatt tctaaagcat tatttttgcg atttttcctt gacatatttt
     1081 catttgacct ataaattttg ccttgccgct gtattgacgc actgactgtt tctcgtaata
     1141 gtacatctgc ttcaatacaa agagaatatt tatgggaggt gcgcaatatg aaccataaaa
     1201 ttaaaatgac agccgatacg caggaagaat tcgaattgat cacccttacc aatggcgatt
     1261 ctgccgatac catatattcg cttcgaccaa ctgtgcgatt cgatggtgcc tacgatgatg
     1321 aattcgacta tgacagcgat gatgacgagt ttgatgacga agaagatttg gaagatgaag
     1381 atgacttcga agaggatgag gaattcgagg acgaggaaga tatcgacgaa gatttcgacg
     1441 aagatgagga cataggtgag gatgaggaat tcgaggacga ggacttcgaa gacgaagatt
     1501 ttgattacga cacggatgaa taagagcgtg gtcttataga tcaaaaggcg cggcgaaaac
     1561 cgcgcctttt aaatatcttg ctcataccaa gattaatgac actattccat tccgaattca
     1621 gattccattt ccacgaaaag cgactgcaaa acggggtgca ttgaaccaga gtgattccag
     1681 atcataaaaa tccctggcct cagcgctcat gatatgaata atcacatcct gggcatccat
     1741 taagagccag cactgatcgt ctgtaacttc aggcttattg agaagatcta ttttttccac
     1801 ttttagaaag tcctcaatgt gcttttgaag ccctcgcata tgagtggaac ttgtgcttgt
     1861 ggccagtaca aaatagtcag cccacccagc tatctcatga acatccataa cgagaacatt
     1921 cagcgcttta tgttgcgcca gaatctcagc actctgatat gcaaacgcct tactgtccaa
     1981 tacaatacct cccgttgaaa tcctttccca atattatagt gatattggcc ttttgaagcc
     2041 ctggcagaac tgaagcatcg ccaaaattct tgcaatttat aatatttgca acctgctgaa
     2101 gcgctgcctt atctccatcg ttgtcgtaca tgactgtttt tgcataatcg aagcttggtg
     2161 cattgcctac agatacaact ttgtagccat agctctcgaa gagaatcgat gctgtatttg
     2221 caagcccttt ttctccacag ccattcagta tgtctattgt cacaatcgtc ttctgagaag
     2281 ctccatcttc ctctataccc agcgcctttg ctgtttgact caccacatcc cgagcaagct
     2341 ctccatcata atatggaaag agaaggcgac gcccctcgaa gcttctaaaa gatccggaaa
     2401 tgaactgcgt aattatcata tcatgatcaa tctgagctaa gtgctcaaaa atgctcaaac
     2461 gggttgcaag gctgaaattg ctctttggct ttgaggcaat caaacgaaga ttactctttt
     2521 tgttgagact tgctgattca tcggcaagcc tccccaacat gttaataagc aacctttgtt
     2581 tacggctgat ctcctcggaa tatgaatccg tagccaaggc ataaagaaga tattggtttg
     2641 tcttatcccc atctagcact acagcgcctc ctggcagaga cacccctttc aatgagtcgc
     2701 tctccatcac agtctcgggg ataaacatct gaattccttc tagcagatct attgtttgac
     2761 aaaggctagc ctcatcgtat acaaaccagc catctatcgg gtatttaaga taatcactta
     2821 tttctttgac aaatttgctg ggatttcgag agtcatagac agtgtcgatg ctgctcatct
     2881 tatttgcgct tttcaagatg atgcccatag tgctaggaat atccataatc gcagccttgc
     2941 gcctcgaagg ataccagatt aaaagttgat tagaaacagg cttcttatta ctctcaaaaa
     3001 taaagagcat agccatgggc tggtctttct tgataatgct ctcgacagtt cccttctcgg
     3061 tgagttttac gacaaagaaa atcccgacag ccgcaacgat caaaatcact aaaagtggca
     3121 aggtgctccg ctttagaatt ttcgatttag agtcgctcat gattttgtca tcctaatata
     3181 tatctcggca gtgtatgggc aaagcggctt cccagattca gaaaaccatc ttacgactga
     3241 agcaagcgta tatgcaaaaa gggaatccag gtccaaagtc tgcatttttt catctgcatc
     3301 cgctgatcta ttgcgaaggg gctcaagctt atccgctatg tatacaatct tagcaagagg
     3361 agacatagca tcatctgcaa tgctatgcaa tgccacagct tccaatatcg attcttccct
     3421 gacctggaac ctataacaga gcgaacaagc tgccgccggg ccatgaatca tcttttccct
     3481 atattctcta tccgataaga ttgctgctag agatgggcgc tcacttagaa aagcgatgca
     3541 agctctatgt ttgtttacca gattgttttg ttcctcaaaa ctcaatcccc tgcacatatc
     3601 atgcgacaat cccgccagat gagcagccca tcgatcttca ccaaaacgtt cggccaattt
     3661 taccgcggtc tctgaagtcg atcgagagtg agcatatctc ttgggagaca aattgcaggc
     3721 aatctctttt tcaataattg acgcaatttc aggtatttcg atatagtcca taattctcta
     3781 tgattgtgcg gacggaaggc ataacgagcg aacgccatgc accgccacca tgaatcctcc
     3841 ctcttacaag cgtggaagaa atgggaataa caagattatc gagataatca tgaggatagg
     3901 gcaatgggat attctcgaca tagagacgat gggcaacgat aatatccgct aattccagaa
     3961 tcttttcggc tcgtttccat gtcgctagaa aatcaggagc tagatcatcg cctatcacaa
     4021 gcccgggctt ttcatcaaac agctttctat cccatagttc ttccagggtg tctgaagtat
     4081 aggatagacc agttcgcttc aattcgcaat cttcgattgc caccttaggc catccttcaa
     4141 tgcttgcttc caagagagca agccgcattt ctggacctgg gtccatctga ggaactttat
     4201 ggggtgcagt cttggctgga ataagcaata ctctctggta acgaccggtg gcaagaatct
     4261 cttcaatcag aatcatatgc ccgatatgag gcggattaaa agaccctcct ataattgcaa
     4321 ttctcattaa ataaagccct cgtccgagaa atctatttca tcgaatatgc gctccttggc
     4381 attttctttt tgagattcat aattctcagc agcctcatag gccaaaacct tttcaaagag
     4441 ttgctgtttg acctcatcca gaccttgacc ggtaaaaaca gatattccat aaatgctctc
     4501 ttctgggaaa gacgctttga aatcaataaa atttgcttgg gattccgata gatccatctt
     4561 tgttcctagc aagatacgcg gtttcttcac cagggttggc gaaaatagtt ccaattcgcg
     4621 caataaagta ggaaaagccg ctttccagtg ctcatcggaa agatccacca gaaaagcaag
     4681 gcaggctgtc cttgcgatat gctttagaaa gcgaagcccc aatccagctc cctcatgtgc
     4741 tccctctatt atgcctggga tatcagccaa gataatatcc ctctcattaa ccgtaagaac
     4801 gcccaaattt ggtatctttg tcgtgaaagg atatggagcg atctttggac gtgcgtttgt
     4861 aaaatactct agtaagcttg attttccagc atttggaaac ccaacaagcc ctatatccgc
     4921 aatgaggctc agttcgactt tgagctttct ctcttgtcct ggctgtccgg gttgagcaaa
     4981 acgcggcgct tgatttactg agctcttaaa atgagtattt ccccagcctc ctttgccgcc
     5041 ctttaaaaaa atccatggct cttcatcctc cagagcccca agctttttgc ctcttgcccg
     5101 atatacaaaa tccctaataa tctcatttgt ctgagcgtcg cgaagaatag tcccaggcgg
     5161 aacttctatt ataacatcct ttccatctct tccatgcatg ttctttccca tgccaggctg
     5221 accgttttcg gctttgaaca cctgtctata tcgaagatat gcgagcgtcc gaagattcct
     5281 ctttacaaca aacaccacat ctccaccgcg ccccccatct ccgcctgctg ggccacccaa
     5341 gggaataaat ctctctcgcc taaaggcaac gcaaccatcg ccccccttac cgcttgcgac
     5401 gcttataatc gcttcatcag cgaattttat cacagaaatt ctccattatt gtgagcataa
     5461 taaaaaaccg ccctctgtgc tacaggagcg gttagaatta tgctctagtt ttaccttcat
     5521 gcttgaatgg ctggctccac atctacaaat ttcttgcctt tatattcatg gaagcgcact
     5581 gtaccgtcga taagcgcaaa tagggtatca tccttaccct tacctacatt cttgcctgga
     5641 tttattttcg aacccctctg tcgtacaata atcgaacctg cgcttatcct ctcgctagca
     5701 taggccttga caccaagaca ctgcggattt gaatctcggc cattaacacc tttatgtggc
     5761 atggtatatt cctccggtta agatggacgc ctcaagcgcc cacaatttca ttgaccgtta
     5821 gcactgtata aggctgacga tgcccacggg ttttgcgata tccttttttt ggcttatact
     5881 taaatacaat tatcttgtca cccttgatct cttctcttac tgtagctttg atagcggcat
     5941 cattgacata tggagttccg acttttgttt catccccact aagcaataac actttttcga
     6001 agacaatcga ctgacctggc attgcatcaa agcgatctac taccagatcc ttcccctgct
     6061 ctgcatgata ttgttttccg ttgatttcta cgattgcgta catttagctt atcctcataa
     6121 atcctcaaat attcatatca ggctgcaatt tatacacttt atgaccgcat cttgtcaaca
     6181 gctctcagaa ccaaaagaaa gcgaatcagt atccgggtct tcttacatgg cgtcgagaaa
     6241 tggtatgcat aacaaattgc ttgctcttcg gagtatttct ttaatcgctg tcactaacgc
     6301 caagcggctg gccgagatat caggattgtc gttcttcagc actggattgt ctcgatacca
     6361 ggcggaaaat tcgctcgcaa gctgatgcgc ataagcagca agtatggaag gatccttgtt
     6421 ttttgccgac tgctcgatag tatagggaaa gtccatcaat agcctcatga gagaccaatc
     6481 ggaatccgac actataagag aaacatcgat tttcccttgg cccgcattac cctcaccatt
     6541 tttgtgtttt ctaagaatag agctggctcg agctcccata tactgaatgt agggccccgt
     6601 atctccagta aaagatagag attgctcagg attgtagagc atgtctttgc ccggcgaaac
     6661 ttgcagaaga aaataatgta aggctccaag ggcaacctgc tcggcaacat cttcgatatc
     6721 gcccaccgct tcctcccttc ccttatccgc tatttcattt gctgcaagtt ctgtaagttc
     6781 gtctatcagg tcatcggcat ctacaaccgt tccttccctg gttttcattc tgccggaagg
     6841 caaattcaca agcccatatg agagatggta gagattctct gcccagctat aaccaagcct
     6901 ccttagaact tcaaaaagta ctttgaaatg atattgctgc tcagaggcca caacatacac
     6961 gagctcatgg aaaggccatc tgttgtaacg cataatcgct gttccgatat cttgcgtgat
     7021 gtagatactc gtgccatctt ttcggaggag tacttttttt cctagaccga catcttccag
     7081 atctacctga attgcaccat cctctgcacg atagaatatt cctctagcaa ggccttccag
     7141 cacttcctgt ttgcccagtt tataggtctc gtgttcgaaa tggaattcat cgaaactaac
     7201 cctctggcgt ctataggtat ccataatgcc ttcgacagcc catttgttca tcttttccca
     7261 tagtgcaatt acatctgggt ccccagcttc ccatttctgc agaagttctt gagctttttc
     7321 ttctgcaaaa ggatcttctt cttttaatct attgaataag acatagtatt tgcctacaaa
     7381 gtgatcgctt ttcaatcctt cgtcttccgg tgtacgtcct tgtccatagg caagatatgc
     7441 gagcatcgac ttgcagatat gaattcccct gtcgtttatc agattgacct ttttaacttc
     7501 tgcccctgct gctttcatga ttctcgaaag agactctcca agcacattgt ttcgaaggtg
     7561 gccaaggtga agaggtttgt tggtgttagg gcaggaaaac tcaatcatta tcctttgacc
     7621 attaaaaagt gggctagatc cccaaccttc tttctcagca ttttcaagga cataattcgt
     7681 cacatattct ttgtctagat atatgtttaa atatgaacct gttgcattaa tggtcccagg
     7741 tagaatgagt ttctcaatat cgattgtcat gttgcagaga ctcgctattt ccgctgcaat
     7801 tctatccggt gacattcgaa attgctttgc atatgagaac atgggaaagc ctatgtcgcc
     7861 aagcttcggc ctaggtggct tctcggcaat gatatcctga gatgaaaaag agacatcctt
     7921 gagctctttt tggagagcga gctcattcag gcagtttgca attatcgatt tccattgatc
     7981 cacaaaatat tccatgagga gctccatttt agcgattttc tctgaagctt tgcaataaga
     8041 tggacttgcg ttttgaacta tgacctcgca atatacgaag attcgaggat gctatatcaa
     8101 aataatccgc aatcaggtct aaaacggcct catttgcttt tcccttttcc ggcgctgcct
     8161 ttactctcag caatatgcgc ccatcgcgca gtccctctac actatcgtaa ggagcacgag
     8221 gaataacata gacatcgagt actatcccct ccttctcttc atggagggct ggctttaaaa
     8281 tatcgagatg ctctagaagc cttgctgaag gtgttctcat tcaaaatcca tcatttaagg
     8341 ccggccatct caaggaattg ctcttcatcg atgatcttaa tcgataattc tttagccttg
     8401 cggttttttt ccgatcctga gcttggatca ttcgtgacca gataatcaag atccttggtt
     8461 acgctggatt ttattatacc gcccttttcc cggaccagtt tctcagcttc cgagcgcttc
     8521 atcgatctca gctcgccagt aaaacaaaaa ctattgccgg agactggatt tgcggtcctc
     8581 tgggttttag ggagcggagg tctgattcgc acatatccac tatcgacgag cttgcgcata
     8641 tcttcctcga ccaatcgcag cccttcgtaa agagtcttgg cgctgatttc agcaaaaccc
     8701 gcgatcttct cgaaatccgc cggtgaagca gctagaattt tatctagagt atcatatcca
     8761 gcttctataa gcttctctgc catgagcaag ccaattcctt ctatatcaaa acctgcgata
     8821 aactgagaaa gactgacttc atttcgttct cttatattgc gcaatatctt ctgagcgctc
     8881 ttccggccta ttctttcaaa ggagataagg tcatctattg taagtgaata tagatccggt
     8941 atgcgcacaa ctctcttagc ttcgaaaagt ctcttcaaca acgttgatcc tatatctttt
     9001 atatcaatta cagaaagcca tttttcgagg cgatgatagg ctttccctgg acattctgga
     9061 ttcggacaat aaagacgcgt atcctcatcg attagccctg cgccacataa cgagcaatgt
     9121 gaaggaatag gaatcggaat agcatctttg gggttttcga ccagagattc gatcttagga
     9181 atgatctctc cgcgcttggt tattacaacc ttagaaccga ttcttaagtc catagagcga
     9241 attatgcctg gattagccag attggcacgc tgaacagtgg ttcctgcaag cctcacaggt
     9301 tcgactatac caataggagt aacggtggtc ccagactcac tccattcaac tgctatgagt
     9361 gtcgtaacag cctcttctgg actgaatttg aaggctatct gtagctcggg acgcatcttg
     9421 ttcatatcct cgaaatcgat tcgatcaccc tttactacaa gtccgtctat gtcatactcg
     9481 agagtaggcc gtaactccat gatcttggca cgatatacaa tgatctcctc aggactctta
     9541 cattcgatgg ttggaacaac attgaaaccc attcttttca gccaggcaat cttttctcgt
     9601 tcggtggaga aggcttcgcc agtgggcaat ctgcccacgg catcatagca tacgatatca
     9661 agatcctcga ctccctgacc atctttgcgt ttcatgagac catttgcggc attgcgacaa
     9721 ttggccttgt cagaatattt ggatgcatgc actctcttcg acatgagcac ttcaccgcga
     9781 actgatcctg taaatggctc tggaagccga ttcggaactc cattcatacg caccgcgttg
     9841 ggagtgatat cgtcgcctat taccccatct ccacgagtga cggctcttgc aaagccacct
     9901 tcatcgtatt gaagctctat gctagcgcca tcgagcttat attgcacgag gtaaagcgga
     9961 tatctgactt tagctgccca ggctaaaaat gactctggat cgcttgcctt tgagagactc
    10021 cccattatca tcctatgcct atacttaggc catctatctg atctgtctgc tcctatagaa
    10081 gcaataatag gatttgctgg atctatcgaa gcgagttcat cccataatgc atcgaattct
    10141 tcatccgaaa tctcgggttc gccgttataa tacagttctt ggtggtgcct gatcagatac
    10201 tccaattctc tgattctctt ctgccgaata tcaggatctt cacttattct caaatcatct
    10261 ggcatgatct tatactaatc atccaaagga agcctgtcaa attactttga gataagatcc
    10321 tattccacat ttcttactga ctggatattc aagatatgct attctatcga catgcgtttt
    10381 tatgctatcc aggtaataac atgcaaagaa gaggaatggc ttgagcgaat tcaacatcaa
    10441 gtaccagata taaaattcca taaaataatg aagaagatgt acataagaaa aaaggggaaa
    10501 gccagacttg acaattcacc ggtttttcca ggctacgtat ttttcgagta tgatggtgat
    10561 tctctgcctt tggagatagt atatcggctg aggcattcca agtctttcat acgattccta
    10621 ccgaacaacg aaggtccgca accactcaac aatcgcgata gcgaaattat tcgccatttc
    10681 atcagttttg gaagcttgat tccgccatcg cttgtaagat tcgacgaaaa tcagagaata
    10741 aaggtaatag aaggaccttt acaaggcatt gagggcttta tcattaaggt gaatagaaga
    10801 aaacgtcgcg caaaagtgag gctcgatata gcagactcta ttatattact cgacctggcc
    10861 tttgaagtca tggaagcgga aacagccaaa gcagctccgc taggtgtata tcatgacaag
    10921 agatgaagct gtcccatttg aacagctctt ggcgcaagat atgctatatg cctttgccat
    10981 aagggcgttc tcaaatattg gaaatatcca gaacatcaat gttcctcctt tgccggaagg
    11041 ctatactctt atcactgctg atcggttgcc atttaaaaac gaacttagaa tagaaggcat
    11101 gagtctgcca ttgttagcag ccaaaaaggt ctcctacata ggagaagtcg ttgggctaat
    11161 cgttggtccg gatccattgc ttacggaaga gctggcattg aatacgatag tcgactgcga
    11221 agcacaagaa tcaagactgg actggcaagt attttcatct tcagatattt cagcccgaat
    11281 tgattattcg cggggaaaag agaaatcgtc ttccgaatcc ctaaaagcct cagaagccga
    11341 agatagtctt gaattggctt cctatttgcg tctggagccc catgaattta gctttcatag
    11401 tggcctgggt gctctcgcag aatgggaaaa cggtagattg aaactcagtt gccccgctct
    11461 ggcgcctgag cttatacact catgtcttgc agagttaacc ggcctctcga ctgaggatat
    11521 cgaaatcctt caatctaaaa tgtatgacaa ttccgggata tatttctggt atccttcatt
    11581 gcttgcagcc agaagcgcag cagctgctag agttctagga aggcctataa agctccttat
    11641 atctattcaa cgagaaaaac gattcctacc tggtatacat ggtatatctc ttcatatgaa
    11701 aacaaggtgg tcaacgcaga aacattgtct tatggatgtt gcatgtcgct ttgcgattcc
    11761 tgttggagct tattctgtct tagatgagct gatattagaa aagactgtgc gtctcgttgc
    11821 ggatgctatg cccaacattc ctttgactat aatcggcttt acggtaaaaa ccaacatgat
    11881 tcctatggga gctctcgact gtatttcatc atctgcgata tttagcatgc tggagactca
    11941 tattgctcgc gccgcaagaa aaatggatgt cgagctccta gaattactat ggatgacctt
    12001 aagcggaata gattcttcct caattatgaa taagatgcca ccaaaggaag agtcttctac
    12061 cgaaaaaatc ttaagacctt tattggaaca tacggatttt catcggaaat acgcagccta
    12121 tgagcaaatt cataagagaa gccatggagc aaaagagact agaatacgag ccatctcttt
    12181 gtctattgct gaacaaagcc atatcgactc gtcttgcatt tcaacaatgg cagatctggc
    12241 aaaaataaga aataattgga ttagctataa acgatatgga gaaggccttc ccattaaaat
    12301 ccagatattt tcatcgatga agcaaagtga cgtctccgat tcccaatcca aaagtataag
    12361 acatctatct caaggagcgg caatccttga ggtcgattat gatagcatta ggtccatctt
    12421 ccatagcctt cgtcttgata tatcgattta tgccggaaaa attctctgcc catccgccgc
    12481 aaagggtgca attcgctctg cgagtatcga agctctcctt gcatgccttt cccgaagcat
    12541 ttatcaagat agaaacatag atgatatata taaatggatc ttttctgagg gcagaatcaa
    12601 tatagaactt atgaaagacg aaaaggccaa ttttgagcgt tctataggag atctcgcata
    12661 taccctcgta ctctcatgct ttctcgaggt gatgcaacaa ttgaacagct cggaaaggct
    12721 tatattgccg ctcaagccta cccctaccgc aatgtactct agcgatcttt tggagaaaga
    12781 tgatgaacat tgagtttttc ttgaatggca atctgcagag aatcgcaatt actagcgtac
    12841 aaagagcctt cgacgtgctt gttgatcaat gcggaataaa gagcctctcc gagatctgtc
    12901 ctgatagcga atgcagtaat tgccttattc tatatgatgg taggcttgta catagctata
    12961 tcctgcctgc ctttcttttg cgggaaaggc atatagagac aatcgaaaat ttcaccaaga
    13021 gcaaggaata tggcattcta tccgcagagt tcgataagga gggcctattg tcctgtgctt
    13081 actgcaataa ttcgctgatg ctcgtagcaa ttcatttgat tcaaagatct ttatctccct
    13141 catggcatga gatatcggat atccttgcca gcttgcattg cagccatatt gatcccagag
    13201 cctttgctga tgcgctctcc aaagtaatac agcaattgcc catagcgaga aaaacgccat
    13261 gaaccttagc gaaatctatt atccgcgtag tatccaagaa ctcatagcca taatggcccg
    13321 acaaaaaaat ctgcagatag ttgctggcgc aacaagcttc ggatatattc aagacacacg
    13381 ttatcttttt tttgagcctt ctatagcaag catcagaaat atacccgaac taaaaaccat
    13441 tcataagact gagcggatga tatccttcgg ggctgcatgt gctctaaacg accttgaatc
    13501 tgtatatcca ttcgaaaaaa aacctgccag agaagtattg agagccaccg caaactccac
    13561 agtgcgaaat attgcaacta taggtggaca tcttatgtat caaaagagat tccttgctct
    13621 ttggcctctg ttggcatgct tagatgcaga acttgagttc aagggaccct caggaacaag
    13681 catcaagaat atatggtacc tgaccaatga agaaggcttg ccttctctgg aatccagaag
    13741 ggttctcaca agaattagga ttccgcttca atctatcgat cgccttttta ttagaaggac
    13801 aggtgcggag attttcccca acggagatgg tgcatatcta gttgcggcct ctaccatcga
    13861 acgcaactcg atttcttatt ttaggctggt tatagccgga cagcgtgcat tccgagatta
    13921 tgatgctgag caaagaatta cctcttcgcc ctatcccctg acggaacgtg tttttcattc
    13981 cgtcttaaag atgtatagcg agagcctcca tcgtaccaaa ttctggaata tggagctttt
    14041 ccttccgctg atatcgcagg cactcgaaga cctgcagcga tcaagtgtat tatagctaag
    14101 gactaaatga gaggtatcat gtcagagaat gacaaaaacc gcggtaccct ttatctttta
    14161 ccggttcctt tattgcctta ttcacctgaa gattggaagc cttgtcaact catgaatatg
    14221 atagccccca atgttattgc cataatgaaa agcgttccat attttgtggt agaatcagag
    14281 aaaacagcca accgactgct ctcccgattg ctttcgccag agcaattctc tgaggtcggg
    14341 ttttttatcc tcaatgagca ctcgaaagaa gaggatctca aggctccgct ttctgcactg
    14401 aaaagcggtt atgactgtgc catactttcc gaggcggggc ttccttgcat agcggatcct
    14461 ggtgcggcgc ttgtatctct agcacatgaa gagcagctgc gaattgttcc aatcggaggc
    14521 gatagctcct tactcctcgc gctcgctgct tctggtctca atggtcaacg cttctgtttt
    14581 ctcggctaca tgccaatacg aagcgaagag cttcaaaaag aactggtcct tgaaggccaa
    14641 gctgccatgc acgataaagc tgcaagaatt ttcatcgaga ctccataccg taatgaaagg
    14701 acattaagag cttgtattga agcactacct gaaaatctct atctttgcgt tgcatgcaat
    14761 cttggaacca atcagccact aataaggtcg atgcccattg catcttggcg aaataaagcc
    14821 attataatcc cagagaagcc agccgtgttt tgctttggaa tacccgcaaa gctcaataag
    14881 aagcagcttc aatcagctag tagagaaaga cgctccttga gataagcgct tttctccttt
    14941 aagccaattg cttttgtctg tatccgaatg gcatttggct tttctgtcga taggcggatt
    15001 gtgcctgcag actcacgtat aagtctcaga attttctccg cggaaatctt cgagaccttc
    15061 gaaaattcca cggtaacgat tcctgctcgc tcctgcaggc gccctatgga gagctttcta
    15121 cataagactc tcatttctgc aagcgaaagc agagagagtg cctcatccgg aaggggacca
    15181 aaacgctctt caagttcctc atgtagagaa tcaatctccc cctgtgtcca aacagaagcg
    15241 atgcgcttat agatttccat tttaatcatc ggagcagaga tatagctatc tggtatataa
    15301 ccagagtact cgagctccag atagggctct tcttcctcat tctggatgcc tgaaagtcta
    15361 gatacggcat cgtcgagcaa ttttagatac agatcaaaac caacagcata tatattaccg
    15421 gattgctcgc gtcctagaag attccccgcg ccgcgaacct cgaggtcttt cattgcaatc
    15481 ttaaagccag agccaagctc agtaaaatcg gatattattt gaagtcgttt catcgcaatt
    15541 tcgctgagag ctctcttatc tgggtataga agataggcat aggcttgtct atcggatcgt
    15601 cctaccctgc ccttaagttg ataaagctga gaaataccat aattatctga tctatcaata
    15661 attatagtgt tgacgtttgg aatatcgata ccattttcta tgatagtagt ggaaacaagg
    15721 acatggaagg ctccatggat aaagcgatgc attatcgact ctagatcttg gccattgagc
    15781 ttgccatggg cactctctac tatggcttcg ggaacaagct gtcggataaa atactcaact
    15841 tcgggcagtg tttcaatgcg attatggaga taaaatatct gaccgcccct cgctatctca
    15901 tttcttattg cgcgggcgac gagttcagga gagaattcct ctacaaaagt ctgaattggt
    15961 tgtctttcca acggagccgt ctgcagaaca gacatatctc taatcttgag tagcgacata
    16021 tgaagggtac gaggaatggg agtcgccgtt aaggttaggc agtctatacc gaccttcatt
    16081 tcctttaagc gctccttgtc tttcacccca aaacgctgct cttcatcgac aacgagtagc
    16141 cctaagtctt tgaaacgtac atccttttga agaatcctat gagtgccaat taccatgtcg
    16201 atgcttccat ccgctaatcc ctttataaca gcattcttag tttttctatc gaccagtcgt
    16261 gagagcagcg ccactttgat tggaaaatct ccagcccgct cgagaaagtt ctcgaaatgc
    16321 tgttcggcta gaatagtggt aggagcaaga aatgcaactt gcttgcctgc gaggatcgcc
    16381 ttgaaacatg ctctcatggc aatctctgtc ttcccaaagc ccacatcacc gcacactaac
    16441 cgatccatgg gcttcgagct ctccatatcg cgtttgacct cttcgatgca ttttagctga
    16501 tcttcggttt cttcatatgg aaatgctgcc tcaaattgga tctgccattc gttatcagat
    16561 ggaaatgaaa aaccacgggc gtttttccga cgagcataaa tccggataag tcgctcagca
    16621 agctcctcca ccgatttact gactcttttc tttctgtttt cccagacttt tgatccaacc
    16681 gagtcgagat ggggagcctg gccttcattg cctatatatc tttgtactag gttagcctgc
    16741 tcgataggca caaagatctt ttcattgcct gcatattcga tacagatata atctcgctcc
    16801 agaccgagga ctatcagacg ctctatcccc gcaaacttac caatgccata attgttatga
    16861 accacataat cacctggagt cagatctata aagctctcga ttacggcact cttcgtcttg
    16921 gcaatagaac gagggacatg tttccgcctg ccaaagagct cgtgctcggc gagaactcgc
    16981 aatttgagag aaggaatggt aaagccagca gaaatgggcc ctttagcgat aacaatagaa
    17041 tcatcctgga tgagcgaact aatccgccct gcttgtagct cagtggaggc aattatccat
    17101 gttttatagt tttctttttt aaaacccgac aactcttctt tgaagagcct tatattcccg
    17161 aaataggagt gtggtggttc tgcttccaaa gatatgcgct ctgctgagcc atcgactccc
    17221 ttcaatgcat gacagaacag cacagaggag aaagcagcaa atagcccatc tggacttata
    17281 tggatcttat ttggcggtgg cactaagact tcccgaagtg caatccgata cattgaggca
    17341 tattctttcc ttaatgtctc ggaaattgca taaatgcgct cgctgcctac tagcacaagc
    17401 atcgccctat tatcgagata atccactagg ttgtacattt tatcaaatgc aagcggaaac
    17461 catagttctt cgccctgcat ctgcttttca ctgtcaagaa tctcgataat ctccttaaaa
    17521 cgaggatcat ttcgcatgaa atccgtagca ttgttctgca gctgatagat cctggttttg
    17581 tcccacacga gttctttgag aggtctcacg tgtacttgag taagcttctc gcgcccggtt
    17641 tgtgtctcag gatcgaaacg gattattctt tcgatacgat catagtcaaa atggatacga
    17701 actgcatatt cataaccagg catatagata tcgagcactt cacctcgcac ggcaaattcg
    17761 cccggcaggc tcacagaagg taccctcaga tatccaagtt ccgctagttt gctggatata
    17821 agggttggtt cgaaatctgc cccgattttc agcggcaaga tatgattttt gatatattct
    17881 ggtggcggaa ccggaattgc aagagtgcgc gccgaaacag ttgcaatttc aaaatctcct
    17941 tccgcgagcc tagcaagagc agaagccctt tccgtaaaca tacgagaata caggggaata
    18001 gccctataag gcgctccaaa ccatgaagga aaactcaaaa ggtcaacgcc cgtaagcgct
    18061 agatcctgag aaaactcttc tgtttcatgg tctgatggaa gaacgacaag gaatcttctt
    18121 ttggttcgct ttaccagagt tgcaaaagac agagcgagca aagaattctc tgaattctga
    18181 atatggagag gaaaggagtt ctgctctatc agcttctgaa tttctcccaa tgctggattt
    18241 ctggatatct tttcgaataa cgcttgcaga ttgtcttttt tcatcgtatg ctgtgtcatt
    18301 gaaggtggat tgaagtatag tcccttttgc gggttttatc catgggtgcc aaaactaaga
    18361 taccatgcta gtacttgtcc ttatcgaaca aacatcgatt gccgaccttg tccttaggca
    18421 attgcgtcag aagagcattg cctcgatagc ttaccgcgat ccactgcggc tattggatca
    18481 tctcgcagag ttaaatcctg aggcggtcat aattcgagga aaagattttc ccttgcatca
    18541 gcagctgctt gcggccttaa tccgttatta tgtaccgttg aatcattgca agatgattgt
    18601 ccttggtagg ggcattcctg cttttccccc atgcgcctgt atcgctgaag aacaatttct
    18661 aaaggatcct tctttactta caaatgatct atcaaaaacc ctccgtatcg attccaggac
    18721 tggaagcagg ctagttgcaa aagcacagcg tactatgaaa ctttgaatat aatctgagaa
    18781 gccagaaagt cggcctgatc tattcggctg ttcggctgtt cggctgttcg gcatgcatat
    18841 cgcattatca gctttttaac ttgagttttt atgataattg ctatataata caaatattat
    18901 gaaccagaca ggaaaaccgt gtcatgaaga aaaactaagg ccttatgcac agaagttcaa
    18961 ggcaattgga catccaattc gtttgaaaat tctttgcctc attgccggtc aagaagtgcc
    19021 ttgtgttggt gatatctggc gctgtctcga tcaacctcaa ccagtagtgt ctcaacatct
    19081 tgccattcta aagcactatg gaatcgtaac ttccgaagta aagaaaacgc gtcgcatcta
    19141 ctctatctgc gatccattta tcaggaatat aattgatgcg atgatctgtc agatcaatac
    19201 ggaaccgaat tctacctccg aaaaatgacg agaattagcg cataactcga agagaacagt
    19261 acgcccaaca ggcctttagc ctgcaatagc attaaagctc agttgaattc gatctcgtct
    19321 tttaatagtg cttcgagttc ttcttcgaat gtaatatcag cctgctcttt ttcaactcgc
    19381 ctcaaaattt ctccgtgctt gaaaataagt accgaattac ctgctcccgt aatacctaga
    19441 tccgcctggc gagcttcgcc cgggccattt actatgcagc ccataacggc gatcgatata
    19501 tctgcgttta ttgaatatag gcggttcatc catcttgaaa caaaggcatg agtgtcgaaa
    19561 gatgcccttc cgcagcgtgg acaggaaata atcctcactc catgcttgcc ttttcctgcg
    19621 caggcaagaa tttccttgcc tgtaattact tcctgttcca tggcatcgga gagagagacg
    19681 cggatagtag caccaatgcc cttctttaaa agtggtacaa gcgctgctgt gttgcggaca
    19741 attccagcta ccagaggccc ggcctcagtg acacctaaat gcagaggata gggatagtgc
    19801 aaagaaaact cttcattggc tcgcacgacc tcatccgggt tattcatctt taaggataca
    19861 acgatatcat ggaagctcat ggattcgagc aactcaatct cgcgatcagc cgcttttatt
    19921 gccgcttgaa tgacatcctt ctcccgagca agatctcgag gcaaagaacc ggcatttatg
    19981 cctatacgaa tagggattgc tttgtctttg gcctttgttg cgacctcgcg gactttccat
    20041 tcggcaccta tattgcctgg atttatgcgg atcttcgcta ttgggaaatc catgcatctg
    20101 agcgctatgc gccaatcgaa atgaatatct gccacgatcg gcatcggaga aaggcctgca
    20161 agtataccaa gcgattccgc cgtggatatt tccggtactg caaaacggat aatgtcacat
    20221 cccagattct taaggcaaga aagctgttca aggacgggca aaagctgcgc ttcttcggcc
    20281 gatggaaata tgggaagcgg attcttccac atggtctgga tacgcaccgg ccattccccg
    20341 cccaagagca catcccctac ttttactgtc agcgtttcct tcatataaaa aagctgatac
    20401 tcgacacctt gaatgctatc caagaattag attgcgctga atgtgaaagc caatacgaaa
    20461 agagccacgg tttcgcacag tcctaccaca attatgtagt tagcaaaacc tttgcctgtc
    20521 tctccaaatg catcacaacc cgcggccgcc gcctttccct gtgcaattgc tgaaacagct
    20581 tcagcgattc ctgcagtgac tcctgaagca agcaatagca gcggatcttt tgtggagttt
    20641 attattctgc tcatcaagat gaagctatat attgtctggg tcaagggagc tcctgcaaag
    20701 gcaacgagca aaaacgatgc agccttatta ttcaaataag agcgtttcca ggatccaatt
    20761 gctgccatgc cagcaattcc tgccccaata ccggagccaa tggcaccaaa tccaagcaca
    20821 cacgcagcac caatcaacgc aatattcata caccactctc ctcaagggta gattgctaag
    20881 acgcggaaag attctcatcc tctttcagcg gatcgtattt gtaaccggac cactccatgc
    20941 cgagatgtcc cgaaaactcc aacatattga gtcgtatgcc gtgcactata accgaaagaa
    21001 ctgtaagcat gaaatttagc gcatgggcag cgactaaaat tacagctgca atgatcaact
    21061 tcaatacgaa accaataaga aaacccgaag gcccgccaag cattcccgag gccattccat
    21121 tgaccgtctg agaaaaagcg agccccgcaa gtcctacagc ccaaagcctt atataggaaa
    21181 cgatatcggc aaagacgctt acggtgccca aaaaagtcgg aattatgttt ttcaagctct
    21241 cgataagact agaacctaac ttaccattcc aattgccgaa tatgaataca agaaagaaac
    21301 ctatggcgat acaaccaatc gcccaattct ctattggata tcgagttgcg tcaatcacca
    21361 gattcaacgc tgcattgaac atgccaagaa gcaacagaag cgagcccgct tgcgacatac
    21421 acttcagatc tgggaaatct cgcttaaaat tctttatgtg agcaattgaa agctgaataa
    21481 ggccaatcag aaagcaaata accttgacat tgttttcaga atccggcgac tgaccattta
    21541 tgagaggaag agatatattt tgaagtaatt taggaagatt gtcgaagttt ataccgaacc
    21601 aggtagcagt cagcgcgccc cagataatag ttgcacttcc caaaagaaga aataacttct
    21661 gtgcatcgac aattggcttt ccttttcttt ttccagcata aatcgaagca atcgttgcta
    21721 gaacgatgat cgctccatag ctagcatccc caaatatcat cgcaaagaat attgagaaaa
    21781 atatcaaaaa ccagctcgat atgtcatact cccaataatt aggcactgtc ccaagaaact
    21841 cgaatacagg ctggattatt ctcactgccg gtgaattttc tataagggtg gggggctggt
    21901 cttcctctac cggatcatcg aaggcaatag cccagccata tttagcggct gtcttcttga
    21961 atttctcgca atcccgcaca ggaacatatc ccttcatata agcaaaactc tcgtgttgcg
    22021 gcatgccgga tcgcaatcgt tctagtatta tgttgtaatc aagcatatgc agatatttct
    22081 taataagaga tagatttgct gagtgctttc gaagttttgc tatgatctcc tgcttttggt
    22141 tttcgatcag ggcaatttct tgtcgcatct gcgaaagcgg aatttcgggc ggcaaaaatt
    22201 ctaagaatga tgggggcaga tctggtatct gatcgccttc gcggacaatg aggcttatcc
    22261 tgcacctacc ttttggagca gaaatcctaa tatattcggt ctcttttggc agtgcaatca
    22321 gctccttagc cggaaaatcg aagaatcgaa gagagaaacc tgctccttcg acgactcgct
    22381 cgagcattgt catcgagact tctccccaac tcgctactct atctatttct ctttttagat
    22441 caatgacttg atcctcaagc aaattaagct tttgcttcgc agcagatatt tcattgatga
    22501 tttccctaac atctttgcat tcttcgtatt tgagagattt cgaagaatcg gccttgcctg
    22561 gtttggattt tttatcgata tagttctgaa gaaaattatg gaccgattca acctccatcc
    22621 tttctcgctc tagattctga tatttatctc cgataccctg aagttcctcc acatgggcta
    22681 caccgagttt tcgaagtcgc tcgggaaggt tttctctatc tctctggagc acgatgaggt
    22741 agaatttttt cataggcact atcatcgttc agctccttcc acctttcttt ttgcgatctt
    22801 gcccctaact acttgagccg tctcctgatc gcccaaataa atgcgaatct ttcgtatgtc
    22861 ggcgatggtc tcgggaattt tcactttttc gaaaaggttt acgcgttgag ttgttattcg
    22921 taactcctgc gaaagcaggt ctttttgctt tgaaaccacc tccagctcca gttgtgtgcg
    22981 gacaagccgc tccataagta tcaaagcagc atcaacccag agagaagtcg aatacagatc
    23041 atagccctta tgttcaaatt ccactccaag gaatagagga atagcaactc ccgcaatgtt
    23101 tccggtatcg gtgctaataa acgcaacctt caagagaggc tcaccttcct gtgtcctcgc
    23161 ggcgccctgc tctgcgaaaa cttccatcca tggagatgca tcttgctcga gtgtcttttt
    23221 aagggcttgg agctcctcca ttcgagtctg gacgctgcgc acttcgactt gaagctgctg
    23281 ttttttaagt tgaagcgttg gaagatagcg ctcatacatc ttgagctcat ctcttttctt
    23341 tttcaactca ttcttggtca gcttgatctt ccccatgatt ctattgcctt ctattcttgc
    23401 tagctggaat tatttctttc ctatccagaa cttttgtatg agatccgaac gaagacctgt
    23461 ctcgcgcggc tcaaagcact cagccaatgt ctcccagcat aggtccaagg ccttctccag
    23521 aggtatgttg acagataaat ccatgatcct ctgctcaaag agttctccat atttgaggag
    23581 cttacgatcc caggcgctca tctggaagcc catagatttc ttctcgaggg tgtctctata
    23641 tgcagcgaat agctttatca tcgcatccat gatagagcga tgatcttccc tggtcttggc
    23701 gttaaccaat tgctttagtc gcgagagaga tccaaaaggc tcaatatgcc catcgcgaag
    23761 atagaactgg ccttcggtta tatagccagt gttgtccgga ataggatgcg tcacgtcatc
    23821 tccgggcatt gtggtgacag ccagaatggt tatggagcct gcatcagaaa aatctacggc
    23881 tttttcgtat cgtgcggcta gttgtgaata caaatctcct ggataaccgc ggtttgatgg
    23941 tacctgttct tgggtaatag cgatttcctt caaagcatca gcaaagtttg tcatatcgct
    24001 aagcaaaacc aggacatttt tgcctttgag agcaaaacgc tcggcaattg ccagagaaat
    24061 atccggcacc atgagacact cgacgatagg atcggccgct gtatgcacga acattatggt
    24121 tttcgacaaa gaacccccct gctccagggt atctttgaag aaaaggtagt cgtcatattt
    24181 gagccccata cctcccagga tgataaggtc gacttcagcc tgcattgcaa tgcgcgcaag
    24241 aagaggattg taaggctctc ctgaaaccga aaaaatagga agcttctggc ttttgacaag
    24301 tgtgttgaac acatctatca ttggaatacc ggttcttatc atctcgcgtg gaataatacg
    24361 ctggtagggg tttacagccg gtccggctat agttatgaga ttctccgtaa gagcaggacc
    24421 tccatcgcgc ggctctcctg caccatcaaa gatgcgaccc atgagattgt ccgaaaagct
    24481 aacttgcatt gggtgcccta agaaccgcac ctcgtcgcct gtcgaaatgc cgcggctacc
    24541 cgaaaaaact tgaagataca ccatctcact atccagacgg attgtctccg ctagagaagc
    24601 accatatcta gtatttactt gcgctagctc accataagca atatcctgcg cctttacagc
    24661 aataacgtta cctacgattg attcgattct cgagtatatc ttattcatgc cttactccag
    24721 cgtgctaatc aatttaagtc cacggtcatc aaactcaggt tttctgtcaa ccagagcctc
    24781 ctccacccta ttatacagag cgctgaacat gtcgctatct tgcggggtac cattcaagtc
    24841 tagcatcgtt tgacgaagct cgtagaaaaa attacgcgcc tcttctttat cggagaattc
    24901 tatgccagct gcaagtatct taagcaccaa cttgaacaca tgcttctgcc ttgccggaga
    24961 tactgctgca tcgacaggat caaatgaatt ctgctgcaag tataccgcat ccaagaattc
    25021 gctcttcaga tagattacat aatcctccag gcttgtccct tcttcaccta caactttcat
    25081 catcgcgctt atttcagctc cacgttccaa gaatgaatga gcatacctaa cagcagattt
    25141 gggcataatt ccatcatact tggtccatga atcaagagga tggatcgaag gatatttgcg
    25201 cgcatccgag cgctcacgcg aaagcccatg aaaagcacct acgactttga gtgtcgcctg
    25261 tgtcacaggt tcttcaaagt taccgcctgc gggcgaaacc gttcctccga tagtaaggga
    25321 tccctccttg ccgttatata gccttactat accggcgcgt tcgtagaatg cagctataac
    25381 agactcaaga tatgccggaa aagcctcttc tccaggaatc tcttcgagtc gtccggacat
    25441 ctctcgcatt gcctgtgccc agcggcttgt agaatcggca agaagaagca cgtcgagacc
    25501 catttgtcgg tagtattccg caatcgtcac agccgtatag accgaggcct ctcgagctgc
    25561 tacgggcata gacgaagtgt tgcagataat aacagtccgt tccatcagag agcggccagt
    25621 ctttggatcg atgatctggg gaaattcctt gagtgtttcg acaacttcgc cagcacgctc
    25681 gccacatgcg gctatcagca caatgtcaac ctcggcatgg cggcttgtta cctgctgtag
    25741 aactgtcttt ccagctccga aaggtccggg gatacaataa gtgccacctt ttgctactgg
    25801 gaaaaacgta tctatgattc gggtttttgt aacaagcggc tctgaaggct taagacgctc
    25861 ggaatagcaa tccacggctc tctttacagg ccaggaaaaa ctcatttgga tatgcctttt
    25921 atttcctttc aaatccacta cttccgctat cgcctcatct accgtgtaat cgccttccgc
    25981 cactatatct gcaactttcc attctccata tagatcaaaa ggcatcatta tgatatgccg
    26041 aaaatatcgc tcaggaaccc agcctatacc cattccgcgt ttcagaatat cgcccttttt
    26101 caagatcggg gtaaaatgcc actttatatc tcgaggaaga gccttgaggt ataggcctgg
    26161 ctctaaaaaa taccctatct tgttagcaat ctccggcaga gggttttgta gtccatcgta
    26221 tatctgcccg agaagacccg gaccaagctc gaccgataat agctcattgg tgaactctac
    26281 tctgtttccg acctttatac ctttggtaat ttcgtagacc tgagcctgaa cggaagtacc
    26341 gcgaatacga attacctcgc tctttagcct tctttctcct accaggatat aagcgacctc
    26401 attcatggtt acaatccctt cggtttggat ggaaaccata ttgccattta ctgcggtgac
    26461 gaatccttct gtcatgttgc tactccggta tcaaaagcgc tcgctgcttc tgctgcagca
    26521 tttacgatat cttggtataa gacattgaat ccttgcatcc cgcgatttct atcgaaggaa
    26581 gcaattcgtg atgcaataag cagtttcatt ttatatgcta gaatataatc aagttcaaat
    26641 gccgaaagcg cggagagcct ttctgttgcg ttccatcgtt cgcgctctaa ggcaagctcg
    26701 gcttggagcg gatccgaagc agattgtgcc gcttgcgctg cttttagaga atccggattc
    26761 atatcccctg gtctggtcca ttggccagca tcttgctttg tagctcttgt tcgatagact
    26821 acaagcgcat tgcgcagccc tctttcaaat tcataatatg acatcaaaaa tggagatttc
    26881 atcatggagt cagagaacct gccattcctt gcctcttcta catataagag aatttctaaa
    26941 tcacttttat ccatcatcct ggagcagagt tcatcaaaag cctgcataga gatgggaggg
    27001 ggtgagccaa atggaaaaga tgtaagtgtt gaagcaaagt accaataatg cttcatttca
    27061 atcgaggctc cccgcagatt cttttaggta ttgagaaagg agcctgttta cacgaaccga
    27121 aagcatttgt gcaatgctgt ctgcagaaaa gtcatattgc accgaggaac cagacaaggc
    27181 tattctgaat ccagcatcga ttccatcgta tggcttgaat gtgacacctc ttgagagttc
    27241 cttggcaagt cttgcagaaa gagaactgtc tattttctcg agttgctctg gtggcaaaag
    27301 cacttcaaaa tcgctcgact ggccggatga gagaatcttc aaaacttcag ggataacctg
    27361 tgcggccatt tcctcatcga aagctttctt gatatcggcg ctgattgctg aatcgagaaa
    27421 gcgctgaaca ctttggcgca gagcaatcat tgtatcccta gatgcttgga gaagactttc
    27481 gcgagaagct gcttccattg catcgatacg cctcaaggcc tcagcttgca tctcctcagc
    27541 gtctctctta gcgcgcgcta taatttctgc tgcttccgat tgagcttttt ggataatctg
    27601 ttctgcttgc tgttttgcat tttcgatgcc ttcgctatga attctctcaa gaagttcttg
    27661 taattggatc tccatattaa ttccctctgc ataaaatcga caacggaaga aaaggatcgt
    27721 atctcatcaa tgcactaata tctacaagaa tatgctcagg gaaccagcaa aaataagtcg
    27781 tcctcttctc aacatcgata aaatatagaa tttattgcca atatacttgc aaattgaaat
    27841 taattttata ctgaaagata gattggggca agagccggtt tggcgattaa tgattagcga
    27901 gagcctactt gacaagcaca ggatcatgcg attaaatcgc ccaggtcgat aagctacgaa
    27961 caagcaaaaa ccagagcagg gtcgggctac ctttgtcttt ggtcgtaaga agaaacgcga
    28021 attgattgcg accgtcgtat aacggctatt accccagcct tccaagctgg agacgtgggt
    28081 tcgactccca tcggtcgctt atatcttgca taatatacat agaatagagc atcttgttca
    28141 tgcagaatgc tgccgtatct agctgatccg agtctgttgc gataaatatc ccaaataaat
    28201 taaaatataa aaagatcttt tgcttttcga tcgaaataaa agatactatt tgctgatgag
    28261 gtgcttggct cagtttccct ggtgagtata atgaattcgg gaataacgat tatgaaacca
    28321 aaagtaaaga cacctattgt ggagcttgat ggcgatgaga tgacaagggt catctggtcg
    28381 atgatcaaag aaaagctcat tctcccctat attgatgtcc atcttgaata ttacgacctt
    28441 ggtattgcga atcgagatgt gacaaatgat cgagtcacga tggacgcggc tcgagccatt
    28501 gccaaatatg gggtaggagt caaatgcgca accataacac ctgactctga aagagtcaag
    28561 gaatatcgcc tcaagaaagc atggccttcg cctaatgcga ctattagggc atatcttgat
    28621 ggtacagttt tccgcaaacc gatcatagca aagaatatag ccccagctgt ccgcagctgg
    28681 cagaagccca tcataatagg aaggcatgcc tatggcgata tctacaaagc agtggatttt
    28741 gtggtaccag gtccgggcac cgtaagcctt gtctaccagc cggcacaact gggtatgccc
    28801 atttctttca aggttcacga atttgaaggc ccaggagtag ctatgggcat acataatacc
    28861 gacgcttcga tccgttcctt tgcccgagct tgtatagaat atgcactttc agaaaaaaca
    28921 gatatctggt ttgcggctaa ggataccatt agcaagaatt atcatggaca ctttaaggaa
    28981 ctatttggcg aggagatctc ggcgcgaagt gccgatctag aagccgctgg aattcactat
    29041 cgatatatgc tcattgatga tgcggtcgct cagctgatga agaacgaagg aggaatcctc
    29101 tgggcttgta tgaactacga tggcgatgtc ttttccgata tggtagcctc tggatttggc
    29161 agccttggca tgatgagttc agtccttgta tctccttcag gagctttcga atatgaggct
    29221 gcgcacggta ctgttcgtag gcactattat gaatacctca agggaaatcc tacgagcact
    29281 aatcccattg cgtctatctt tgcgtggaca ggagcgctta gaaaacgtgc tgagctcgat
    29341 aaaacacccg agataacaat ctttgcgcaa cagcttgaag atacggcaat tgaactgatc
    29401 gaatcgggtg tggtcacaaa ggacctcata aaccttataa gcccacctgc aaagagcttt
    29461 gaaacaactg aatcattttt ggataagatc gatgctggcc tcgcccggaa gcgaaccgca
    29521 ttgcaggctt aattgaaaag ctagatccct agatgcttca agtcttgtgg gtctaaatac
    29581 cttctacaag gaaatgacag caaagttcaa gtaacagaac tcaggtaaca atgggtattt
    29641 gcaagaacaa tattatgtaa ttccttatgg gatcagcgcg actcgaacgc gcgacctact
    29701 gcttagaagg cagttgctct atccagt
//
LOCUS       DAOOWY010000012        29384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1591_length_29384_cov_12.066794, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000012 DAOOWY010000000
VERSION     DAOOWY010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 29384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29384
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1591_length_29384_cov_12.066794"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PK105_01960"
     CDS             complement(<1..181)
                     /locus_tag="PK105_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOU60501.1"
                     /translation="MRKLVAVILALAFLVAGADVFAQAKAKFHIGVVTGTVSQSEDDL
                     RGAEQLIKEYGSVKDG"
     gene            328..1689
                     /locus_tag="PK105_01965"
     CDS             328..1689
                     /locus_tag="PK105_01965"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU60502.1"
                     /translation="MQFSWKRFADELFYYSNQFILFFVLIISLTSGSWKNALVTSLIV
                     LSFMLIQTSLLTIQGHIPILRFLYSFITAAGYSILRATLSDIAFTETVNLLLWGASVY
                     IAVFQTLTLILHKPILKRFSEAALSLGSAIIFLLFYAYLDLRISVTASFEKGQIDAEA
                     LAQALSLKSFLAVFDKFIQAPQHLFALFGVISFDFMLMVARWRAINLEQRLTRIIEMP
                     QEAKKIYISSLEPETDASSAESESQEVQLAASPENQENPPAKSAKTVASLFVTVISSD
                     IIGFTDLSEQFGHKEAAALLNRYYAIWTRCAGSYGGRIISISADTVVIVFGLVDEEHS
                     ADRALNASYAFIEELEGLKEDMIIKSLPGEIKVSLGIHSGYVTRALLGPPGQQKMSFF
                     GDTIGIAARLDSLCREFHQNLLVSHATYRHLTLESQVTLERFSEALLRKSTRPMPLYT
                     KKP"
     gene            1707..2483
                     /locus_tag="PK105_01970"
     CDS             1707..2483
                     /locus_tag="PK105_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60503.1"
                     /translation="MRLGANQKRNGWRSVVSFISIIALIVFISFSVTSCSGNLDATIK
                     GDSSVRAAIRLDIPDALSVRVRQFVGLKPQEPLFSTEAVRKQFLGQTNIALVDVSSPT
                     PDILTSVVWVQNLEALIADTSLVPMGMICYQKISPPGQSPAMRELSVNLSRENAPAML
                     KLFPGIDRRIIDSLSPPALEADPVSAAEYRLNLEQLIIGKKNMPAFDACAVNIAIATP
                     KMIVSATGGSISGTVFRMRIPLFSLLTLEKPISFSLRWHE"
     gene            complement(2452..3450)
                     /locus_tag="PK105_01975"
     CDS             complement(2452..3450)
                     /locus_tag="PK105_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU60504.1"
                     /translation="MLYTFDFHNHSCLSPCASLEASPSEMVEWAIKKRIDLFALTDHN
                     SALNSPAFGIECANAGIIPLFGIELNPFEEAHLLAIFSDPLTALRFSDWVFEYLPAMR
                     VDPLQFGDQVVVDPLGQILSMPSSIWYGSSLKESFTFFAQEAAKAGAIVIPAHIDRPH
                     FSVYSQLGFLPQGPYDAVEAMSPNPDPTLCGDFCVISNSDAHVLEHIGRRPSIIELDE
                     KKVIDMHAALSKMAEAWFRGLWTNQANISASSPEKESEEDSEEDIQDQTSEAGLSIEI
                     PGLLPIISFLEEWYPFAEAQALFENIRQSLHRQKAWSIYKKPKPGSLIHASAEKKK"
     gene            complement(3467..3814)
                     /locus_tag="PK105_01980"
     CDS             complement(3467..3814)
                     /locus_tag="PK105_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60505.1"
                     /translation="MTTKDVPDIISAEVLQGDFEDRAVKIVYTSDLLSDVLANGRDAS
                     VLVTIQAHKNTVAVASVVDIPLIVLCNNRPIIDDMIETAAQSRIGLVRTALSQFEVSG
                     RLWQALHVPGSPR"
     gene            complement(4026..4532)
                     /locus_tag="PK105_01985"
     CDS             complement(4026..4532)
                     /locus_tag="PK105_01985"
                     /inference="COORDINATES: protein motif:HMM:NF021612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HOU60506.1"
                     /translation="MPNLSSPEHRLFFCMNIIYAKRMNSLSVQTQYWIMAISIIVFAI
                     TIITAVLAYKAGKKHGFLDAQVSENERMEHARKDAIERSRAVLSGQISEQLAPWLPDF
                     PVNPSDARFIGKPIDFVAFCGTDEGSVSEIVFVEVKSGKSALSYVERSVRDAIVGGHV
                     RWVEYRIE"
     gene            4609..6468
                     /locus_tag="PK105_01990"
     CDS             4609..6468
                     /locus_tag="PK105_01990"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOU60507.1"
                     /translation="MKTLKMSPSYVKNERLLSWVSSMEELCKPDLVYWCDGSDEEYDT
                     ICRSLVASGTFIALNPEKRPNSFLSRSDPSDVARVEDRTFICSKDKADAGPTNNWVEP
                     NEMRGILNKIFAGCMRGRTMYIIPFSMGPIGSPFSYIGMEITDSAYVVANMRIMTRMG
                     RKVIEALGENGEFVPCMHSVGMPLEPGQKDVAWPCNKQTKYIVHFPETREIWSYGSGY
                     GGNALLGKKCFALRIASVIARDQGWLAEHMLILGLESPSHEKTYVAAAFPSACGKTNF
                     AMLIPPESFEGWKVTTVGDDIAWVRPGKDGQMRAINPESGFFGVAPGTSEKSNPNAMA
                     TIRANTIFTNCALTPDGDIWWEGMTKNPPEGLIDWQGRPWDPASGKPAAHPNARFTAP
                     IKQCPSIDSAWDSHEGVPIKAFIFGGRRSTVYPLVYQSFNWIYGVYLAATLGSETTAA
                     ATSAIGLVRRDPFAMLPFCGYNMASYFDYWLHVGLYNPNPPLIFGVNWFRKDADGNFI
                     WPGFGENIRVLKWIVDRANHKANAIESPIGWMPRYEDLNWEGLDFPKERFIELMSVDR
                     ELWENEILSHEELFVKLYERLPKELIFVRQLLLSGLWRAPEHWKSDTIDSTIN"
     gene            6510..6848
                     /locus_tag="PK105_01995"
     CDS             6510..6848
                     /locus_tag="PK105_01995"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOU60508.1"
                     /translation="MRVFYIVLLIAVAVLAVIFAVQNSAPITVSFFGWSAQASMSIVL
                     VLTFAAGILIGMLLLFPSIWKRMRALSAQKRKTSEARKLAKETQETKASPSPEAGQEE
                     SLSASHDTLN"
     gene            complement(6934..7152)
                     /locus_tag="PK105_02000"
     CDS             complement(6934..7152)
                     /locus_tag="PK105_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOU60509.1"
                     /translation="MTKNMGKTDKRVRIIVAVVIAILILTGVLKGVLAWILGIIAAFF
                     VVTAAISFCPLYTLSGCSTIHKEKTEKK"
     assembly_gap    7413..7512
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7513..9648
                     /locus_tag="PK105_02005"
     CDS             <7513..9648
                     /locus_tag="PK105_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU60510.1"
                     /translation="IIVLIKQVPETDKVKMDPDTGTMVRSGLESIINPLDLYAIEEAL
                     ILKERFGAHVTTISMGPLDAERSLRESLAMGCDEAILLTDKAFAGSDTWATSKVLAEA
                     IRHIDNADLILAGERATDGDTGQVGPAVASWLEIPVLTYVSSLEIESKTEDLETAESN
                     RNRTCHPTFLVRRLTEEGYQSVRVQSPCLITVVKEIASPRLPTLRGKKFSRSAEILHW
                     GAKEINLDPASLGLAGSPTRVVKIFYPKVSRDGERIHALDEPGIEKAIERILSMIRPV
                     IGSPIMGDRGSRESRRGYTETSPAVDIEESAIGVLIAKMQPALQESVISLSEKVFSEP
                     ATDPKPEFWIIAERRKRGLDIVSFELLARARPLADSRGAILAAIVLGSASPDEAAMLI
                     AHGADSVILVENPSLEDFVCELWSETLLKLAYARKPEVILGAATTTGRTLMPYLSAKL
                     GTGLTADCTELSIEENSGLLLQTRPAIGGNIMATIKTAHHKPQMATVRPHSMSPLPPD
                     FGRKGIVIHVIDESSKERIISDFSDSRVQVLALESNVKDFENLESASIVVAGGRGLKK
                     ADNFKLIRELASALGGAVGASRESVDRGWTSYPHQVGLSGKTISPEIYICAGISGAVQ
                     HLAGIRTAKKIISINTDPDAPIHAVADLAIIGDLFEILPRLTERLKPIYSSSYPSLSS
                     SPYQTKDTRSQEPRILDSKTLSTEAQHEI"
     gene            9638..11056
                     /locus_tag="PK105_02010"
     CDS             9638..11056
                     /locus_tag="PK105_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOU60511.1"
                     /translation="MKYNPISRDIIRELINIAGERNVLLDPAQLEIFSHDETSKEEYG
                     HMPEVVVTPRSTSAVAEIVKLANRTSVPITPRGAGSGLSGGAIPIFGGIVLSLEKMNQ
                     VREIDYDNLTMTVETGIVTNEINNLVKEKGLFYAGYPMSLETCTLGGNIAENAGGGKA
                     IKYGVTSRYILGLEFVTPTGEVVWLGGKLAKDVTGYDLVHLIVGSEGTLGIATAAIIR
                     LIGLPAAKSDLLVLYRSPQEAISSVPKILSKGLIPTSIEFMDRRSVETSCTYLNENLP
                     YAQCGAMLLIEMDGRDQSLVESEAEAVGDLCMEQGAIEVYVADNRTTQERLWAIRRNI
                     AEALKVYCPVQSLEDIVVPPTAISAVMPELERIALQFGIAIPCYGHAGDGNLHATLVK
                     DPQMPMEQWQQIEQQALLALYESITALGGKISGEHGIGLKRKHFMRKFMSPVELNLIK
                     AIKRAWDPNLIMNPGKIFDIED"
     gene            11261..12166
                     /gene="pdxS"
                     /locus_tag="PK105_02015"
     CDS             11261..12166
                     /gene="pdxS"
                     /locus_tag="PK105_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOU60512.1"
                     /translation="MEQKLWSDRQSEQWHIKVGLAEMLKGGVIMDVTNAAQAKLAEKA
                     GACAVMALERVPADIRAQGGVARMSDPKIIKEIQDAVSIPVMAKCRIGHFVEAQILEA
                     LGIDFIDESEVLTPADDRYHVYKHPFKVPFVCGCRDLGEALRRIGEGAAMIRTKGEAG
                     TGDIIEAVRHMRTVMDGIARLLGLPEEELMTEAKELGAPFELVWEVAKTGKLPVPNFS
                     AGGVATPADAALMMQLGAESVFVGSGIFKSSNPERRAKAIVSAVVNYKDPKILAEISE
                     DLGEPMVGIGLDELIENQKISGRGW"
     gene            12163..12747
                     /gene="pdxT"
                     /locus_tag="PK105_02020"
     CDS             12163..12747
                     /gene="pdxT"
                     /locus_tag="PK105_02020"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOU60513.1"
                     /translation="MSSHRIGLLALQGDYEAHGKALVRAGFPRCDMVEIRSFSDLQSL
                     TAIIIPGGESTVMSMLLERFGMFEELKRQIENGMPVLATCAGLILLAKNIEGNDQMGL
                     GVLDIKVKRNAYGRQVDSFHAPVQTIIDSVGIIEGIFIRAPRILASGKEVEHLAIYEN
                     EPVMVRQGSIIAATFHPELLPDAPVHRWFIQTFV"
     gene            complement(12697..13383)
                     /locus_tag="PK105_02025"
     CDS             complement(12697..13383)
                     /locus_tag="PK105_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60514.1"
                     /translation="MDKKRFFYVAIYAIGMAYLEASIVVYLRKLYGISDLMISVPAFD
                     PMVSIIELGRELATLIMLLAVGLVSGKKFQSQIGFSLYAFGLWDIWYYIWLVIFIGFP
                     QSLFDWDLLFLIPLPWWGPVIAPVMISVLMIISGVRLALLEEKGLLVRLKWLDWAMLA
                     LGSGTMLYAFMSEAISSLPASVENLSDFRPSSFLWPIFLIGYAISIYATWRILYKSLN
                     EPAMHRSVRK"
     gene            13533..14279
                     /locus_tag="PK105_02030"
     CDS             13533..14279
                     /locus_tag="PK105_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60515.1"
                     /translation="MNKDHGRTKIKQKKNSIDHALPLISKDRVRTNLLKRQEQKALAF
                     LVQYIPSWMSSNVLTGIGFLGNVIVFLSFILAELFDTRFLLIGVIGFAISWFGDSLDG
                     RIAYYRKKPRKWYGFMLDLVIDWLGIVLIGLGFVIYADRFLKLLGYAFIVLYGLEIII
                     ALLRYKISGSYSIDAGKLSPTETRILISLFLVIEVIFPGSIVYLTAIADIILAISNIS
                     EFKKLAKIANERDEEENRKSELKIAENNKE"
     gene            14290..14700
                     /locus_tag="PK105_02035"
     CDS             14290..14700
                     /locus_tag="PK105_02035"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU60516.1"
                     /translation="MAWLRSALTFSKAQISAFVGGVIDYCTMIFITEVFHIHYTISIA
                     AGGMVGAAVNFSVNRTWSFYSKDAQYEFSVIQQLVMFFPVVLGSIALKSAGTFAFTTL
                     SKIDYKITRLAVDVVVSIFYNFMLQKHWIFKKRH"
     gene            complement(14764..16062)
                     /locus_tag="PK105_02040"
     CDS             complement(14764..16062)
                     /locus_tag="PK105_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU60517.1"
                     /translation="MISEILVLLALIFINGFFSLSETALVSSRKARLKAEADKGKSTY
                     KLALNTRESPSRYLSTIQVAITLIGILTGTISGTTFSQLLAQWLSGWSFVGRYAESVA
                     VAIVVLTITFLSVVIGELVPKSIALRNPEYIAALTIKPMHALSVVFYPVVRFLSSLTD
                     SLTKLFRFSHGAEPAITPEEVRILLEQGEKSGVFETAEREMMEEVLNLDDRRVTMFMT
                     PRTEVVTLNIKDSRDSQIRAIIKNSEYAYLPVVDGDLDHTIGMVAVKRALTCMAEGCF
                     LSLETCMEPAVMIPESLSGLQALAILRDAKRSAGLIVDEFGGISGIVTIADLLEAIAN
                     LSEVETEEASQIIKRSDGSYLVDGSMPIDEFAESIGINLDVISNEDYDTVAGFVLHCG
                     GSIPKAGDIIEWPPLKMEIVDMDGKRIDKVLVQKSEQSLT"
     gene            16367..18475
                     /locus_tag="PK105_02045"
     CDS             16367..18475
                     /locus_tag="PK105_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU60518.1"
                     /translation="MKTILDRNRRHMPSRLLSLILICLSTLNLQAQQSQPFSGELPSF
                     DNFEFAFPEGDALRNITKPVFLGEKTFIARLEQRTEFPDFDKNQNSEPRPPLGIVLCG
                     GSARAYAHIGVLKILEKANIYPDFIVASSMGAVVGMLYAAGYSPQDIEFIIKSLPLES
                     FFTPVIPTNGGLINTDIFKAILRKIIGRLDVSETAIPIIIIAEDLKSRQQIWIAEGPF
                     DLVMASAFAMPAIFEPQEFDNFVLIDAGATTIAPVEPALIFSDRLVISTAFYNRAMDF
                     SSPVTVINRAVDIGKTRSGMAGIERSKALVIRNDVENLSYMQFTDPDIIIEKGELSAQ
                     NALEQLDLISRKDLENPPSPEFFELRSEMHENLNNTIQNLQSGQQPSTSLMLRGIPIL
                     RLFDPFISMPGETGTEARIGASLAFSYLKFKSYLSYFNALNPDPGKVWAIETAFRVNP
                     LDSLNAWFTARLWGDYSSTSILSHDSSCWELASRMIWMKAQEERKRGFHIGGDIFFKM
                     DFSIANWQTCAFFQTEMPRKYEKSGPSIQPWYSAYIGAFAENSVGLKNAVGLQGSLQG
                     GFDSKWVSPKFRAFSKVSLNNQEFMESKYDGFRSIATRKAVNSNLITNVEIPFSPHEL
                     FLDISEALLLKGFELAPFFDTRWNSTSDENFHMNDWAAGLSFSFEARAFGLAPATMTF
                     YASYSGSKIISLQFRAGMLL"
     assembly_gap    18575..18674
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19110..19643)
                     /locus_tag="PK105_02050"
     CDS             complement(19110..19643)
                     /locus_tag="PK105_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60519.1"
                     /translation="MKRSKIFMVACIMIVAILCPLLAQNVTNEVHAEVGEIHALGGIG
                     FGWRGFGVSGGMEYILQKFTIPNFPLSLGVMGIAGMDFGKGFDLSAAAMATLHWGMKA
                     YKDFPEFFRNFDWYLGLGMGVGILSDFGFGISTGGGLSYYVNDKFAIDLHSYYINYFV
                     GGGSGFSETIGIRYAVK"
     gene            19774..20343
                     /locus_tag="PK105_02055"
     CDS             19774..20343
                     /locus_tag="PK105_02055"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOU60520.1"
                     /translation="MIRCPWCGSDPVYIQYHDKEWGSPVHDDQIHFEFLLLETQQAGL
                     SWRTVLGKREAYRNAFADFEPQIVAGFREEDIANLLANPKLIRNKRKLEAAVKNACAF
                     CAIQKKYGSFDSWIWHFVEGHPIINHWQDISEIPVQTELSDLVSNEMKKAGFAYVGSI
                     TIYSHLQAIGIINDHLISCFRHAEIAGAK"
     gene            20449..20967
                     /locus_tag="PK105_02060"
     CDS             20449..20967
                     /locus_tag="PK105_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOU60521.1"
                     /translation="MLSQRMIDRINLQINKEIYSAYLYLAMAAKMNELGYSGVGKWLT
                     VQYHEEMFHAMKFAEYLHDQNAEVSYAKIDNPEFKEKEIKPLFQHVLAHEESVTASIR
                     EIMELAITEKDYATQILAQWYIDEQVEEEKNATEIIQKIDLLGNSHQGLYLLDSELAK
                     RESSVPLDFNKI"
     gene            21226..22857
                     /gene="thrC"
                     /locus_tag="PK105_02065"
     CDS             21226..22857
                     /gene="thrC"
                     /locus_tag="PK105_02065"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOU60522.1"
                     /translation="MAVRYTSTRDARIDVSVAEAIVQGMPPDGGLFVPRNVPRLSADA
                     ILEPRLSYAELAWLVLSPWFDWRETELRPLIEQAYSSSGEKSNFDTPEIVLLSNAGSL
                     NCENKYSGEKQSSKLFLLELFHGKTCAFKDLALSLLGGLLSKSLEKCGIDEPVLVLTA
                     TSGDTGSAALSGLGGQQGIRIAVIYPADGTSEIQRLQMTSVPLEDRCVIGLKGTFDDA
                     QRIVKNIFQNAKNLRSPFFGIRLSSANSINIGRLLPQIVYYVKAWRDLYSLGAIDYGG
                     AFDVVVPSGNFGDILAARYAKAMGLPIRRLVCASNSNRILYDLFATGIYDRRRSFNKT
                     ISPSMDILVSSNLERLLYHAFCENSELVSRLMRQLENEGFFLIPQQFAANISDFCAGW
                     ADDLQTLESIRHMWEIHNLLIDPHTAVACKVAHELFSNSQNHQKDRTIMKTQVVNPIV
                     IAATASPFKFPASCCAALQSGLPNPPISISKNDLSRAFELSQATGISVPKPIADLVTA
                     KINHPIIVEESALESVLADFAHKTANSHACRKLTI"
     gene            22865..23950
                     /locus_tag="PK105_02070"
     CDS             22865..23950
                     /locus_tag="PK105_02070"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOU60523.1"
                     /translation="MIEIEVPASCANLGPGFDCLGIALSLSNIVRFEPAEKTQIKGCP
                     MEWADEDNLFLRSLRHSCELLQTSVPKISAEIITGIPPARGLGSSASLAVAGAAAALL
                     LSGKTAKSLKDLANTAVGTVTSLGTEVDMMGDAVGDTETEFNLRAYTGTEVDRTCLTR
                     FLHEEENQRFLLRAATDIEGHADNAAPAIYGGFTAAALTDKGIVISQNPAPEHWQFIA
                     CIPDFILETSLARKALPDFYSRQDVVHGISHAVLVALAFLKGNLDLLNLACSDRIHEP
                     YRRPLIPDFEIVETACFEQGAKMVCISGSGPTIMAVFETSVPDVASSTTDILNETIET
                     QIAKSATHKWIVLPLRVDNLGIRARKI"
     gene            23979..24635
                     /locus_tag="PK105_02075"
     CDS             23979..24635
                     /locus_tag="PK105_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOU60524.1"
                     /translation="MNTQNMIKSQDKRFWLRPQFLPTFYYNIPSSFGEILLIWHDTDT
                     PMIHCIYLPKNGVSRIAQAKTDLNLLEPWRKEVPLPQHIEQILQLLLKMLSGHPVQVP
                     LQIIEHSLSNLKLPEFQQRVLLTEATIPFGYISSYKELAKACGRQLSFRAVASSLSHN
                     PLPLLIPCHRIVASNGNLAGYQGGIEMKCSLLENESIPIRSNKVIMEKAKFWDYSSNS
                     "
     gene            complement(24918..25613)
                     /gene="rsmG"
                     /locus_tag="PK105_02080"
     CDS             complement(24918..25613)
                     /gene="rsmG"
                     /locus_tag="PK105_02080"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOU60525.1"
                     /translation="MDQQQLLARGLAALDFDISSGVLEKLLRYVDELEQWNPVYGFVK
                     AKGQDLIIKHVLDSLGPWRILEDLFSEMDKQFSDNNKLRIADIGTGAGFPGIPLAIAF
                     PQRSFLLIERLEKRTRFLENVICILGLENVEIHQNTVEDEDENLECAIFRALRPFTDK
                     KLFRTIWKKIKLGGALCAYKGRIMHAKLELTGLLDDPVLSNMAAHAKITPVWVPFLEE
                     ERCLVIARKTLNI"
     gene            25783..27390
                     /locus_tag="PK105_02085"
     CDS             25783..27390
                     /locus_tag="PK105_02085"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOU60526.1"
                     /translation="MRKLLFVTGGVCSSLGKGIAASSIGALMEARGFNVQMVKIDPYL
                     NVDAGTMSPYQHGEVYVTDDGAETDLDLGNYGRFTTAKLSRSNSITTGQVYKKVIEKE
                     RAGDYLGRTVQVVPHITDEIKERILAVASNPDVDMTIVEIGGTVGDIESVPFLEACRQ
                     LIHELGKQNAISIHVTLVPAVSSGEIKTKPTQHAVKELQEIGIQPDALILRSSKPLSD
                     ELRRKISLFTNVEEEGVISGYDATTIYEVPLIYYSQRLDTFLLRKMNVESRHAELNKW
                     QTVVQAITKPNRKVKIAVVGKYMDLQDAYKSIWEALIHGGIAYHAGIDIERIDSSRLE
                     DPRADLDAIFKDIHGILIPGGFGERGIEGMICAARYAREKNIPYFGICLGMQIMVIEY
                     ARSILGWQDAHSTEFNKSTQHPVVCLLEEQKKMKNYGGTMRLGAYDAVTKVGSNIRAA
                     YGVELISERHRHRYEVNSDIRLELEAHDLRISATTVDGELIEACEWPNHPWSVGVQFH
                     PEFKSRPTAPHPLFREFIGAALSLSRL"
     gene            27452..28027
                     /gene="lptC"
                     /locus_tag="PK105_02090"
     CDS             27452..28027
                     /gene="lptC"
                     /locus_tag="PK105_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOU60527.1"
                     /translation="MGVYRREEWLIKCIFSLCLLFALIALLSSCSQVTEENSLTAKKD
                     IPDAQFYDLIRKEYREGKLSMQFSAKHASWYETDRRLEVEELAFITYNGDDGSISAYG
                     DAQHAILYEISGDAEFSGHVHLQSAEGDACFEAFELSYRRSLDVFETPFDAEVLIKIG
                     NQLLLAGNGLLFDVKQQYYHIEKNVHGSVNQ"
     gene            28047..28769
                     /locus_tag="PK105_02095"
     CDS             28047..28769
                     /locus_tag="PK105_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOU60528.1"
                     /translation="MKSKIFALILSFMMIFVPFSIGFAQTNPPNVQPSPEPPMQGQQT
                     QPSSGKKPIVFSANTVQGVIAKGKENTILMGSVHVTTGSLTITADRIDLSGEDYVNVV
                     CLGNVTVLDTEKGFSLVAEKLNYHRDTEIGIAQRKVYVNDTKNNTIIEAEWLRFDQKQ
                     SIFEAAVTVQVRKEDMSISSEYALYNRDTEEIWLHGGAIAVTEDGVLKGDVISSSSDW
                     SKLKISGEVSGTITPKETATSQ"
     gene            28766..>29384
                     /gene="lptB"
                     /locus_tag="PK105_02100"
     CDS             28766..>29384
                     /gene="lptB"
                     /locus_tag="PK105_02100"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOU60529.1"
                     /translation="MTDAILEVFNLRKSFGPVLAVSDVSFSMKEGEIIGLLGPNGAGK
                     TTVFYIIAGFLKPNSGSVRLNGVIINNLPMYRRAILGISYLPQESSIFRRMTVEENIL
                     AVLEVRSGLSSAARKEKAYSLMDEFGIVHLAKQPAYTLSGGERRRTEIARALAVEPKF
                     LLLDEPFTGIDPIAIREIKSLIHNLSNKGIGILLTDHNVRDTLAIT"
BASE COUNT         8379 a         6670 c         6338 g         7797 t
ORIGIN      
        1 caccgtcttt gacgcttccg tactccttta tgagctgttc cgctccgcga aggtcatctt
       61 cagactgaga gactgtaccg gtaacgacac cgatatggaa tttggccttg gcctgggcga
      121 atacatcggc acccgccact agaaaagcta gtgctagaat aactgctaca agctttctca
      181 tacgtcctcc ttatttgata ttaatgatgt caaattattg tacaccttaa aatgaacgag
      241 tcaagtattt ctttgttcgc gcaagcaagt ttgtttggcc aatcttgggt ctttccagta
      301 aatgctagga catatacaat agattcgatg cagttctctt ggaaacgctt tgcagatgaa
      361 ctattttatt actcgaatca atttattctc ttttttgtgc tgataatatc gctcacctcg
      421 ggatcgtgga aaaacgcact tgtcacttcg ctgatcgtgc tttcctttat gttaattcaa
      481 acctcactgc tgacaattca agggcatatc ccaatcctga gatttttgta ttcttttatt
      541 acggccgcag gatactcaat tcttcgcgca acgctgtcgg atatagcatt cacagagaca
      601 gtaaacttgc tgttgtgggg agcttcggtc tatatagcgg tttttcaaac actcacgctt
      661 attttgcata agccgattct caaacgtttt tcagaggcag cactatcatt aggatcggcg
      721 ataatttttc tgctctttta tgcctatctt gaccttcgca tatcggtgac cgcctcattc
      781 gaaaaaggac aaatagatgc tgaagcattg gcacaagccc tgagcttaaa atcttttctc
      841 gctgtcttcg acaaatttat ccaggcccct caacacctat ttgcactatt cggtgtaata
      901 tccttcgatt tcatgctgat ggttgcaaga tggagagcaa tcaaccttga acagcggctc
      961 actagaataa tcgaaatgcc acaggaagct aaaaaaattt acatatcttc tttggagcct
     1021 gaaacagacg catcttccgc agaatctgaa agccaggaag ttcagctggc agcatctcct
     1081 gaaaatcaag agaatccgcc agccaaatcc gccaaaactg tggcttctct atttgtgacg
     1141 gtgatttcat ctgacattat tggttttacg gatctttctg agcaattcgg acacaaggag
     1201 gcggctgcct tactcaacag atattatgca atctggaccc gatgcgctgg tagttatgga
     1261 ggaaggatca tatcaatttc cgcagacacg gttgttatag tctttggcct tgtggatgaa
     1321 gaacacagcg cggacagagc ccttaacgct tcatatgcct ttatcgaaga actcgaaggc
     1381 cttaaagaag atatgattat aaaatcgctt cctggggaga tcaaagtctc tttaggcatt
     1441 cattctggtt atgtaaccag ggcacttctt ggacctccgg gtcaacaaaa aatgagtttc
     1501 tttggtgaca caattggtat cgccgcacgg ctggactcgc tctgcaggga atttcatcag
     1561 aaccttcttg taagccatgc gacctatcgg cacctaacct tggagagcca ggttacgctc
     1621 gagcgtttta gcgaagcact acttcgaaaa agcacgcggc ctatgccgct ttacactaaa
     1681 aaaccataat tggagcaaga tttgtcatga gattaggagc gaaccaaaaa cgtaatggat
     1741 ggcgaagtgt tgtttcattt atttcgatca ttgccttaat tgtcttcatc tctttctccg
     1801 tcacctcctg ttcaggaaat ctggacgcta caatcaaggg ggacagctct gtgcgtgcgg
     1861 caattcgcct agacataccc gatgccttga gcgtgcgagt tagacaattt gtcgggctga
     1921 aaccgcaaga acctctattt agcactgagg ctgttaggaa acaattcctg gggcaaacaa
     1981 atattgcgct tgttgatgtt tcttcgccca cgccagacat tctgacatca gtggtatggg
     2041 tgcagaatct cgaagctctc atagcagata catcgcttgt gccaatggga atgatttgct
     2101 atcaaaaaat ctcaccacca gggcaatctc cggccatgcg cgagctttcc gtcaatcttt
     2161 cgagagaaaa tgcacccgcc atgctaaagc tttttcctgg aatagacaga cgaataatcg
     2221 acagcctgag ccctccggct ctggaggccg atccggtttc ggcagctgaa tatcggctca
     2281 acctcgaaca gcttataatt ggcaagaaaa acatgcctgc tttcgatgca tgcgccgtaa
     2341 atattgccat agcaacgcct aagatgattg tgtcggctac aggcggaagc atatctggaa
     2401 ccgtttttcg catgagaatc cctctgtttt cgcttcttac tctggaaaag cctatttctt
     2461 tttctctgcg ctggcatgaa taagagatcc tggcttcggc ttcttgtata tgctccaggc
     2521 tttttgtctg tgtagagatt gcctgatatt ctcaaaaagc gcttgtgctt cagcaaaggg
     2581 ataccattcc tccaaaaagg atataatagg cagaagccct gggatttcta tcgaaaggcc
     2641 ggcttcggat gtttgatctt ggatgtcttc ttccgaatcc tcttccgatt ccttttccgg
     2701 tgatgaagcg cttatattcg cctgatttgt ccataggcct ctaaaccagg cctcagccat
     2761 ttttgaaagg gctgcatgca tatcgatgac ttttttctca tcgagctcaa ttatggaagg
     2821 cctgcgccca atgtgctcaa gcacgtgcgc atcggaattg gagatgacgc agaaatctcc
     2881 gcagagtgtt ggatctggat ttgggctcat cgcttcaact gcatcatagg ggccttgcgg
     2941 taggaaaccg agttgactgt aaaccgaaaa atgtggtcgg tctatatgcg ccggaattac
     3001 aatcgcgcct gcttttgctg cctcttgggc aaaaaaagta aaggattctt tcagagagct
     3061 gccataccaa attgaagagg gcatagaaag gatttgccct agagggtcta cgacaacctg
     3121 atcgccaaac tgcagaggat cgactcgcat ggctggcaaa tattcaaaaa cccagtcgct
     3181 aaagcggagc gcggtcaatg gatccgaaaa aattgccaac aagtgcgctt cttcaaaggg
     3241 attcaattcg atgccaaata atggaataat gccggcgttc gcacattcta taccaaatgc
     3301 aggagaattc agtgcactgt tatgatctgt aagcgcaaat agatcgattc ttttttttat
     3361 agcccactcg accatctccg aaggagaagc ttcaagactt gcgcatggac tgagacagga
     3421 atgattgtga aaatcgaaag tatataacat ggacaatata tgtccatcac cttggtgacc
     3481 ctggaacgtg caaagcctgc cagagccgac cagaaacttc gaactgacta agagccgttc
     3541 gcaccaatcc tattcgagac tgagcagctg tctcgatcat atcatctatg attggccgat
     3601 tattacacag gacaattaga ggaatatcga ctacactcgc cacggcaacc gtattcttat
     3661 gtgcctgaat cgtgacaagc acacttgcat cgcgcccatt tgcaagtaca tcggacagca
     3721 aatcagaagt atagactatc ttgactgctc tgtcttcaaa atctccttga agtacttcag
     3781 cagaaatgat atcgggaaca tctttagtcg tcatgcaatg gtactccttc gttcttgctt
     3841 ctatttcggc tttgccatca tttgaataag aagcttgcat cagatcgatt tcggtatggc
     3901 aaagcgaatg gcaatagaat tacttatatt atagccgcac tttgcatttc aataaagctg
     3961 gaagaatgct ttccagaaca aagcaaaagg ccttgctatt tccttggcat tctttgattt
     4021 gttttctatt cgattctgta ttccacccag cgcacatggc ctccgacaat cgcatccctg
     4081 acgcttcttt cgacataact caaagccgat tttccggact ttacctctac aaagacgatt
     4141 tcgctcacag atccctcatc tgtcccacag aaagcaacga aatctatagg cttgccaata
     4201 aaccttgcgt cggaaggatt gaccggaaaa tcggggagcc atggcgcaag ttgctccgaa
     4261 atttgaccgg aaagaacggc tcttgatcgt tctatcgcat cttttcgcgc atgctccatt
     4321 ctttcattct cagagacctg cgcatctaag aatccatgtt tcttgccggc tttgtaggcc
     4381 aaaacagctg tgataatggt tatggcgaaa acaataatcg atatggccat tatccaatat
     4441 tgtgtctgta cagaaaggct attcatgcgt ttagcataaa tgatgttcat gcagaagaaa
     4501 agacggtgct ccggcgatga taaatttggc attgaagacc ttccccatac acaaaataaa
     4561 aaaaaagcct ataatagcat ttatatcttt attcttcgga gtaatttcat gaaaacgctc
     4621 aagatgagtc cttcttatgt aaaaaacgaa cggcttcttt cgtgggtttc ctctatggaa
     4681 gaattgtgca aacccgatct agtctattgg tgcgatggtt ccgatgaaga atatgataca
     4741 atttgccgat ctctagtcgc gtcaggaacc ttcatcgcac ttaatcccga aaagcgccca
     4801 aattctttct tgtcaaggtc cgaccccagc gatgttgctc gagttgaaga ccgtactttc
     4861 atctgcagca aggacaaagc agatgccgga cccaccaaca attgggtaga gcctaacgaa
     4921 atgagaggta ttctcaacaa gatcttcgca ggctgcatgc gaggtcgaac gatgtatatc
     4981 attccctttt caatgggacc tataggttct cccttttcat atatcggaat ggaaataact
     5041 gattctgcct atgttgttgc caatatgcgc ataatgacaa ggatgggcag aaaagtcatc
     5101 gaagctctcg gcgaaaatgg tgaattcgtg ccttgtatgc attcggttgg catgcctctc
     5161 gagcccggac aaaaagacgt cgcctggcca tgcaacaaac agacaaaata cattgtccat
     5221 tttccagaga ctcgtgaaat ctggtcgtat ggctctggct atggtggcaa tgctcttctc
     5281 ggtaagaaat gctttgcgtt gaggatagct tctgtcattg cgcgagatca gggttggctg
     5341 gccgaacaca tgcttatact tggccttgaa tcaccctctc atgaaaaaac ctacgtagcc
     5401 gctgcctttc caagcgcctg tggcaagact aattttgcta tgctcattcc gccggagtcc
     5461 tttgagggct ggaaggttac aactgtcggc gatgatatcg cctgggttcg tccaggcaaa
     5521 gacgggcaaa tgcgagccat caacccagaa tctggtttct ttggcgttgc cccagggact
     5581 tcggaaaaat caaatccaaa tgcaatggcg actattcggg ccaacaccat tttcaccaat
     5641 tgtgctctca ccccagatgg cgatatatgg tgggaaggca tgaccaagaa tccgcctgag
     5701 ggcttaatcg attggcaggg aaggccgtgg gatcccgcct ccggcaaacc agccgcccat
     5761 ccaaacgcca gatttacggc tccgattaag caatgcccct ctatcgactc cgcctgggat
     5821 agtcatgaag gtgtgccaat aaaagcattt atattcggtg gacgcagaag cacggtctat
     5881 cccctggtct atcaatcctt caactggatc tatggagtat atcttgcagc tacccttggt
     5941 tccgagacta cggctgccgc taccagcgcc attgggcttg tgcggcggga tccttttgca
     6001 atgctcccat tttgtggtta caatatggct tcgtacttcg attattggct gcatgttggc
     6061 ctttacaacc ctaatccgcc actcatattc ggtgtaaact ggttccgcaa ggatgccgat
     6121 ggtaacttca tatggccggg ttttggtgaa aatatacgcg ttctcaagtg gattgtcgat
     6181 cgcgccaatc acaaagccaa tgcaatcgag agccctattg gctggatgcc acgctatgaa
     6241 gatttgaact gggaaggtct cgattttcca aaagagcgct ttattgagct gatgtctgtg
     6301 gaccgcgagc tctgggagaa tgaaattctt tctcatgagg agctgtttgt aaagctctat
     6361 gaacgcctgc caaaagagct catcttcgtt cggcaactct tactatcggg gctctggcgc
     6421 gctcctgagc attggaaatc tgataccatc gacagtacca taaattaaga gtaaagacct
     6481 tcttaagaaa ctccatatga ggagggaaaa tgagagtctt ttacattgtt cttctgattg
     6541 ctgtggctgt tttggccgtc atctttgcgg tacaaaatag cgcacccatt accgtttcgt
     6601 tttttggatg gtctgcccag gcttcgatgt cgattgttct agtattgacc ttcgccgcag
     6661 ggattctaat tggtatgctt ctgctttttc cgtcgatatg gaagcgcatg cgagcgctat
     6721 ctgcacagaa aagaaaaact agtgaggcga gaaaactagc aaaagaaaca caagaaacca
     6781 aggcttcacc atctccggag gcggggcagg aagaatcctt atctgcctct catgatactt
     6841 taaattaagg gcagaatatc aagatgaccg ccataaccga ttggcggtca tctttgtcgc
     6901 cttgctcaag aagctctttg aaccattttt gtcttatttc ttttctgtct tttccttatg
     6961 aatagttgaa caaccagaga gagtatatag cgggcagaac gatattgcgg cagtcaccac
     7021 aaagaatgcc gcaataattc cgaggatcca tgcgagcaca ccctttagga ctccagttag
     7081 aataagaata gcaatgacca ctgcgacaat aatacgaacc ctcttgtctg tctttcccat
     7141 gttctttgtc atattcctcc tccgtgcaga aaaataaatg actcgcccac atgaaatgga
     7201 aacaagaaca aacacaaata taataaaatc gatcagttta gttaaatcca ggttgaagag
     7261 ataaatctat gtctggccag gattaaagat attcaacgat tatctgataa ggcaagcacc
     7321 acaattgtaa ttcctctcca attcatgtct cccaaaggta ttaaagtgca agcttgcgga
     7381 tatatctact cctatgtcat acttttgtgt cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnatcattgt ccttataaag caagtaccag aaacagataa agtcaagatg
     7561 gaccctgata caggcacaat ggtgcgatcg gggctggaaa gcataatcaa tcctcttgat
     7621 ctatatgcaa tcgaagaagc actcatactt aaagagcgat tcggagccca tgtaaccaca
     7681 atttcgatgg ggccactaga tgccgaacgc tcgctgcgag aatcgcttgc aatgggttgt
     7741 gatgaagcaa ttctacttac agataaggct tttgccgggt ccgatacttg ggcaacatcg
     7801 aaggttctag ctgaggcaat aaggcatatt gacaacgccg atctaatcct cgcaggagag
     7861 cgcgctaccg atggcgatac cggacaagta ggaccagccg tagcatcttg gttggaaata
     7921 cccgtcctca cctatgtgag ttcattggaa atagagtcca aaactgaaga tttagaaact
     7981 gctgaatcaa atcgtaatcg gacttgccat cccacatttc tcgtccgtcg ccttaccgaa
     8041 gaaggctacc aaagtgtgag agtccaatct ccgtgcctaa taaccgtagt caaagaaatt
     8101 gcctcacccc gtctaccaac tctgcgcggg aaaaaattct caaggagtgc agagattttg
     8161 cactggggag caaaggaaat aaacctggat cctgcttcat tgggtctggc tggctctcca
     8221 acccgtgtag taaagatatt ttatccgaaa gtatcacgcg acggggagcg aattcatgct
     8281 ctcgatgaac cgggaataga aaaagcgatt gaacgcattc tttcaatgat ccgtcctgta
     8341 ataggcagtc cgataatggg ggacagagga tccagagaat ccaggagagg atacacagaa
     8401 acaagtccag ctgtggatat cgaagaatcg gcgattggag tccttatagc aaaaatgcag
     8461 cccgcattgc aggaatcagt gatttctctt tccgaaaagg ttttttcgga gcctgctact
     8521 gaccccaagc ctgaattttg gattattgct gagcgccgta agcgcggact cgacatagtg
     8581 tctttcgaat tgttggctag agcccgtcct ctcgcggata gtcgtggtgc aattctagca
     8641 gccattgtgc ttggatccgc atctccagat gaagctgcga tgttgattgc ccatggtgct
     8701 gatagtgtaa tcctggtcga gaacccgagc ctagaggatt ttgtctgtga attgtggtct
     8761 gaaacgctct taaagcttgc ttacgccaga aaacccgaag tcattctagg agcggccact
     8821 acaacgggaa ggactttaat gccatatctt tcagccaaac tcggcaccgg actaactgcg
     8881 gactgtaccg agctctctat cgaagaaaac agcggcctcc ttttgcagac acgtcctgct
     8941 attggaggaa atatcatggc cactataaaa actgcccatc acaagccaca gatggccacg
     9001 gtaagacctc actccatgtc accacttccg ccagactttg gccgcaaagg cattgtaatt
     9061 catgtaatcg atgaatcatc taaggagaga ataatctcgg atttttcgga ttcacgtgtc
     9121 caggttctgg ctctggagag caatgttaag gatttcgaaa atctggaaag tgcgagcatc
     9181 gttgttgcag gcggaagagg tttaaagaaa gccgataatt tcaagctcat tcgtgagctg
     9241 gcgtccgctc tcggaggagc ggttggcgca agccgtgaat ctgtcgatcg cggatggaca
     9301 tcctacccac atcaggttgg ccttagcggc aagaccattt cacccgagat ctatatatgc
     9361 gcaggaattt ctggcgctgt tcaacatctg gccggcataa gaacggccaa gaagataatc
     9421 tcgatcaaca cagaccccga cgcgcccatc catgcggttg ccgacctcgc cataataggg
     9481 gacctcttcg agattttgcc gcggctaacg gagcgattaa aaccgatcta tagctcttct
     9541 tatccatccc tctcttcatc tccataccaa accaaagata caagatctca agagccaaga
     9601 attttggact caaaaacctt aagcacggag gctcagcatg aaatataatc ctatcagcag
     9661 agatataatc cgcgaactta taaatattgc aggtgagcga aacgtcctgc tcgatccagc
     9721 tcaactcgaa atcttctcac acgatgaaac atctaaagag gaatatggcc atatgccgga
     9781 agtggtggtg accccaaggt cgacctccgc cgtcgctgag atcgtgaaac ttgctaatcg
     9841 aacctctgtc cccataacac cacgaggcgc tggcagtggc ctttctggag gagctattcc
     9901 tatatttggc ggaatagtat tgtcgctcga aaaaatgaac caggtgcgag aaatcgatta
     9961 cgacaatctt acaatgaccg ttgaaactgg tattgtgaca aacgaaatca acaacctggt
    10021 caaggaaaag ggactattct acgctggcta tccaatgagc ctggagacct gtacgctcgg
    10081 cggaaacata gccgagaatg ctgggggtgg gaaagccata aaatatggcg taacatcgcg
    10141 ctatatcctt ggtctagaat tcgttacacc aacaggcgaa gtggtctggc ttggaggcaa
    10201 gcttgcaaaa gatgttactg gctatgatct agtgcacctc attgtcggct ccgaaggtac
    10261 tcttggcatt gcaaccgcag ctataattag actgattggt cttccggcag ccaaatccga
    10321 cctgcttgta ctctatagaa gcccccaaga agccatttcc tctgtcccca agattctctc
    10381 aaagggtctc attccaacat ccatcgaatt catggaccgc cggagcgtgg agacatcctg
    10441 tacctatcta aacgaaaatt tgccttatgc ccaatgcggt gcgatgctgc tgatagaaat
    10501 ggatggacga gatcaatctc tagtagaaag tgaagcagaa gcagttggag atttatgcat
    10561 ggagcagggt gcaatcgaag tatacgtagc cgacaaccga actacccagg aacgcctctg
    10621 ggctattcgc agaaacatag ccgaagcatt aaaagtctat tgcccagttc aaagcctaga
    10681 ggacatcgtg gtgccgccca cggccatatc tgcggttatg cccgaactag agcgcattgc
    10741 tttgcaattt ggtattgcaa taccatgtta tggccatgct ggcgatggaa atcttcatgc
    10801 gactctagtc aaggatccgc aaatgccaat ggagcaatgg caacaaatag agcaacaagc
    10861 tctccttgcg ctatatgaat cgatcactgc gcttggcgga aaaatttccg gcgagcatgg
    10921 cattggactt aaacgcaaac attttatgag aaaattcatg agccctgtgg aactgaatct
    10981 aatcaaagcg atcaaacgcg cctgggaccc aaatcttatt atgaatccag gaaagatttt
    11041 cgacatcgag gactaatccc gcttaggtaa cagcaagaat tactccatag acattctcaa
    11101 aaaccgaata taaaaagaat actcctggag cgcaatatct cataaatcag aatcctagag
    11161 ctttgccaca acccttgcgt aataagcttt ttttcgctac tctttattct tgcaaccaat
    11221 caaattcctt ccacacagct aacagtgagg agattatatt atggaacaga aattatggtc
    11281 cgatcgccag agcgagcaat ggcatattaa agtcggactc gctgaaatgc tcaaaggtgg
    11341 cgttattatg gatgtaacca atgctgccca agcaaaactc gctgaaaaag caggggcttg
    11401 tgcggtgatg gctcttgagc gcgttccagc cgacattagg gctcagggag gcgttgcccg
    11461 catgtccgat cctaagatca taaaagaaat ccaggatgct gtctcaattc cagtcatggc
    11521 aaaatgccgt ataggccatt ttgtagaagc acagatacta gaggctctcg gcatcgattt
    11581 cattgacgaa tccgaagttc tcacccctgc ggatgatcgc taccacgtct ataaacatcc
    11641 tttcaaagta ccatttgttt gcggttgtcg tgacctggga gaagcactgc ggcgaattgg
    11701 cgagggtgca gccatgattc gcacgaaggg agaagctggt actggggata tcatcgaggc
    11761 tgtgcggcat atgcgcacag ttatggatgg aatcgctcgt ctccttggcc ttccggagga
    11821 agaactcatg actgaagcaa aggagctcgg tgctccattt gaacttgtat gggaagtcgc
    11881 aaaaactggc aaattgcctg tccccaattt ctcggctgga ggagtcgcta ctccagcgga
    11941 tgcggctctg atgatgcaac taggtgcgga atccgtattt gtaggctcag gcatcttcaa
    12001 atcaagcaat cccgagcgac gagcaaaagc tattgtatcg gcagtggtca attacaaaga
    12061 cccaaaaatc ctggcggaga tctcggaaga tcttggcgaa ccgatggttg gcattggcct
    12121 ggatgaactt atcgaaaatc agaaaatttc ggggaggggt tggtgagcag ccatcgcata
    12181 gggcttctcg cgcttcaggg agactatgaa gctcatggta aggctcttgt gcgagctgga
    12241 tttccgcgct gcgatatggt ggagattcgg agcttttccg atctgcagtc cttgaccgca
    12301 attataattc ctggaggtga aagcacggtc atgagtatgc tgctcgaacg ttttggcatg
    12361 ttcgaagaac taaagcgcca aatagagaat gggatgcctg ttctcgcaac ctgtgccgga
    12421 ctaatactcc tagcaaaaaa catcgaagga aatgatcaaa tgggacttgg tgtcctggat
    12481 atcaaagtca agcgcaatgc atatggccgt caagtagata gctttcatgc tcccgtacaa
    12541 accatcattg acagcgtagg cataatagaa ggcatattca tacgggcacc gcgtatactc
    12601 gcttccggaa aagaggtcga acaccttgca atatatgaaa atgagcctgt aatggttcgt
    12661 cagggctcga tcattgccgc gacatttcac cccgaactac ttcctgacgc tcctgtgcat
    12721 cgctggttca ttcagacttt tgtatagtat tctccaagtt gcatatatag atattgcata
    12781 tcctataagg aagattggcc ataagaatga gcttggtcta aaatcgctca aattttccac
    12841 actcgctggc aaacttgaaa ttgcttcgct cataaaagca tacagcatgg tgccggatcc
    12901 taaagcgagc attgcccaat ccaaccattt aagccttaca aggagacctt tttcttcaag
    12961 caaagcaagc cttactccgc tgattatcat gagcaccgat atcatcacag gcgcaatcac
    13021 aggcccccac catggtagtg gaataagaaa gagcagatcc caatcaaaca atgattgagg
    13081 aaatccgatg aaaatgacaa gccatatata ataccaaata tcccaaagcc caaaagcata
    13141 cagagaaaaa ccaatttggg attgaaattt cttgcctgat acaagtccaa cagcgagaag
    13201 cataatgaga gtcgccaatt ctcgcccaag ttcaatgatc gataccatgg gatcgaaggc
    13261 tggcacactg atcatcaaat cagaaatgcc atacaacttt ctcagatata ctacaattga
    13321 tgcctcgaga tatgccatcc cgatcgcata gatagcgaca taaaagaatc ttttcttgtc
    13381 cataatctca tactccaaat aatataaagc ccttttattg acacattcct aaaatataga
    13441 tacttttata ccccgttgcc atacgaaaat cagattgagt ataattgtta aaactttttc
    13501 aactgaagta aagagagatg ttgaggcaga gaatgaataa agatcatggt agaaccaaaa
    13561 taaaacaaaa gaaaaactca atagatcatg ctctaccgct tatttccaag gaccgcgtta
    13621 gaacaaatct gcttaaaagg caggagcaaa aagcccttgc ctttctggtt caatatatac
    13681 cttcatggat gagctccaat gttctaacgg gaatagggtt tcttggaaat gtgatcgttt
    13741 ttttgagctt cattctcgca gagctttttg acactcgctt tcttctgatt ggtgtaattg
    13801 gttttgcgat ttcctggttc ggcgattcac ttgatggacg aatagcctat tataggaaaa
    13861 aaccaagaaa atggtacggt tttatgctag atcttgtgat agattggctg ggtatcgtac
    13921 ttatcggcct tggattcgtt atctacgcag accgctttct aaaactcttg ggatatgcct
    13981 ttatcgtgct ttatggtcta gaaatcataa tagctctgct tcgatataaa atcagtggta
    14041 gctattccat tgacgccggt aaattgagcc cgaccgaaac caggattctt atctcgcttt
    14101 tccttgtgat tgaagttatt ttcccaggaa gcattgtcta ccttaccgca atagccgata
    14161 ttatcttagc gatttccaat atttctgaat ttaaaaaact agcaaaaatc gccaatgagc
    14221 gtgatgaaga agaaaacaga aagagtgaat tgaaaattgc cgaaaataat aaagaataga
    14281 caaaaagctt tggcttggct acgttcagcc cttactttct caaaagcaca aatatctgct
    14341 tttgttggcg gagtaattga ttactgtaca atgatattca ttacagaggt ctttcatatt
    14401 cactatacta tttcgatcgc agccggaggt atggtgggcg cggccgtaaa tttcagcgta
    14461 aacagaacat ggagtttcta ttcaaaggat gcgcaatatg agttttctgt aatccaacaa
    14521 cttgtcatgt tcttcccagt ggttctaggc agtatcgccc taaagtcagc tggaaccttt
    14581 gcttttacaa ccctaagcaa gatagactat aaaatcacaa ggcttgcggt agatgttgtt
    14641 gtatcgatat tctacaattt tatgcttcaa aaacactgga ttttcaaaaa acggcattag
    14701 agtggaatct ttggactatc ggactttcca atctatgcgt tatttgtgca gactagaaaa
    14761 agcctaggta agagattgct ctgacttctg taccaatact ttatcgatac gtttgccatc
    14821 catgtccaca atttccattt tcaaaggagg ccattctatt atatctccgg ctttgggaat
    14881 tgatccccca caatggagaa caaaaccagc aacggtgtcg taatcctcgt ttgatatgac
    14941 atccagattg atacctatgc tctcggcaaa ttcatcgatt ggcatcgaac catccacaag
    15001 ataagagcca tcactgcgct taataatctg agaagcctct tctgtctcta cttccgagag
    15061 atttgcaatg gcctccagta aatcagcaat tgtcacaata cccgagattc ccccaaactc
    15121 atcgactatc aaaccagccg acctcttggc atcgcggaga atagcaagcg cttgcaagcc
    15181 cgataatgac tccgggatca tcacagctgg ctccatgcat gtttccaaag ataaaaaaca
    15241 gccttccgcc atacatgtga gtgctcgctt cactgcaacc atacctattg tgtgatccaa
    15301 gtctccatca accacaggaa gataggcata ttcggaattc ttgataatag cccttatttg
    15361 agagtctcga gaatctttta tattcaacgt tactacctcg gttctaggtg tcatgaacat
    15421 cgttactcgc ctatcatcaa gattcaacac ctcttccatc atttctcgct ctgctgtctc
    15481 aaatacccct gatttttctc cttgttctaa taggatcctt acttcttccg gagtgatagc
    15541 aggctcggct ccatgcgaga atctgaaaag cttcgtaaga ctatcggtaa gagaagagag
    15601 aaatcttaca acaggataga aaacaacgct caaagcatgc ataggcttta ttgtcagagc
    15661 agcaatatac tcaggattgc gtaacgctat ggatttagga acgagttcac cgataacaac
    15721 agagagaaag gttatggtca aaaccacaat agccacagct acagattcgg catatctccc
    15781 aacaaaagac catcccgaga gccattgagc tagaagctgc gaaaaagtcg taccgcttat
    15841 tgtgcccgta agtatgccaa taagtgtaat cgctacttgg attgtcgaaa gataacggct
    15901 tggagattcc cgcgtgttaa gggcaagctt gtaagtgctc ttgcctttgt cggcctcagc
    15961 cttgagcctg gcttttctag atgaaacgag ggctgtctca gagagagaaa agaatccgtt
    16021 aataaaaatg agagctaata ggacgaggat ttcagagatc attgcgcacc tcgtctccag
    16081 atataggagc tattgccatt aattcaacat atttaggacc aatgcatatg aaatagtcag
    16141 agtcacttga agatgagcta tcgctttcca tataaaaaga ataacaaata tccatcgcca
    16201 ttgtcgaggc attaacaatg ctccgataat cttcacttga aaaaactcat aagagccatc
    16261 ggaaaatcac acagccacaa tgacagctga aaaagaccta cggtatttaa aaattctcaa
    16321 aaatactctt ccgtcttcat ctaatatcaa tatatactga cagtaaatga agactattct
    16381 tgatagaaat cgcaggcata tgccatctag gcttctatcg cttatcttaa tatgtttatc
    16441 aaccttgaat cttcaagctc agcaaagcca gcctttttct ggcgagcttc catcttttga
    16501 taattttgaa ttcgctttcc cggaaggaga tgctcttcga aatatcacaa aaccagtgtt
    16561 cttgggagaa aagacattca ttgcaaggct agagcaaaga acggagttcc ccgacttcga
    16621 taagaaccaa aacagcgagc cgcgcccccc tcttggaatt gttctctgtg gaggatcggc
    16681 gcgagcttat gcgcatatcg gcgtcctaaa aatattggaa aaggccaaca tttatccgga
    16741 tttcattgtt gcaagctcca tgggagctgt tgtcggcatg ctatacgctg ctggatattc
    16801 accccaagac atcgaattta taatcaaatc gcttccgctt gagtctttct ttactccagt
    16861 gatacccaca aatggtggtc tcatcaatac cgatatattc aaggctattc tgaggaagat
    16921 tatcggaaga ctcgatgttt ctgagacagc gattcctatt atcataatag cagaagatct
    16981 caaatctcga cagcagatat ggatcgcaga aggtccattt gatcttgtaa tggcaagtgc
    17041 gtttgcaatg cccgctattt tcgaacctca agaattcgac aattttgtcc tgatcgatgc
    17101 aggagccacg accattgcgc ctgtagaacc tgccctcata ttcagcgacc gccttgtcat
    17161 ttcaaccgcc ttttataacc gagcgatgga tttttctagt ccagtcacgg tcattaaccg
    17221 agctgtagat atcggaaaaa ctcgatcagg tatggcagga atagagagat caaaggccct
    17281 ggtaatccgc aacgatgtgg aaaacctatc ttatatgcaa tttactgacc ctgatataat
    17341 tatagaaaaa ggtgaattga gcgcacagaa tgctctcgag caattagatc ttatttctcg
    17401 caaagatcta gaaaaccctc cttctcctga attcttcgaa ttgcgcagcg aaatgcatga
    17461 gaatcttaac aataccattc aaaacctcca aagtggccaa caaccctcca cctccctcat
    17521 gctgagaggc atacctattc tgaggctttt tgatcctttt atatccatgc caggagagac
    17581 ggggacagag gcacgcatag gggcatctct tgctttctct tatttgaagt tcaagtccta
    17641 tttatcttat ttcaatgctc tcaacccaga tccaggaaag gtgtgggcaa tagagactgc
    17701 ctttcgtgta aatccgctag acagcctcaa tgcatggttt actgcccgtc tatgggggga
    17761 ttatagctca acctcgatat tatcgcatga ctccagctgt tgggagttag caagccgtat
    17821 gatctggatg aaagcacaag aagaaagaaa acgaggattt catataggag gcgatatctt
    17881 tttcaaaatg gatttttcga tcgcgaattg gcagacatgt gcatttttcc agacagagat
    17941 gccgagaaaa tacgaaaaat caggcccctc tatccagcca tggtactcag catatatagg
    18001 agcttttgcg gaaaattctg ttggattaaa aaacgcagtg ggtttacaag gttctctcca
    18061 gggaggattt gattccaaat gggtgagccc caagtttcgt gctttttcga aagtgtcact
    18121 caataaccag gagttcatgg aatccaaata cgatggattt aggagtatag caacaagaaa
    18181 agcagtaaac tccaatttga tcacaaatgt ggaaatccca ttttcgcccc atgaattatt
    18241 tcttgatatt tcagaagcct tgctcctaaa aggctttgag ctggctccat tcttcgacac
    18301 gcgctggaat agcacatcgg atgaaaattt ccatatgaac gattgggctg caggattgtc
    18361 cttttcattt gaagctcgcg cttttggact tgctcctgca accatgactt tttatgcaag
    18421 ttattcaggt tccaagatca tctcgcttca attccgggca gggatgctct tataagaata
    18481 aactcagtat aaaatcgtcg aggtcgcgaa aatatcctag aagcctctta accatcgatt
    18541 tttatagtcc ccggtggtct tggtgatcct tgggnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnggtctt ggtgatcctt gggggctgat tggagtctgc tcggttggac
    18721 tgctcgggtg ctcggggaca gaggttggga ccaaggagaa tgtatggatg agattaggat
    18781 ttcgaggcta cttagatgat gctcgatgct cggcggggtt ctcggcgggg acagaggttt
    18841 aatcttcgtt agtcttgacg gctttgagat tgaggatagc gcctagtact tggctttgct
    18901 ttggggacgg aggttggatc atggatgatc aaaaatatag ccaagaaaga tggctaacaa
    18961 aattcgcttg aagccataaa tgaacccatc aactgatgac catcataaag cttaaagtct
    19021 acaaaaaagc acgcaactct caattatagc tgcgtgcatt gtggggatca aaagtgatca
    19081 tgaaaaatat caataataaa gcaatgaact catttcacag catagcgaat gccgatggtt
    19141 tcgctaaaac cagaacctcc tcccacaaaa taattgatgt aataagaatg cagatctatc
    19201 gcaaatttgt cattcacata gtaagatagt ccacctcccg tggagatacc aaaaccaaaa
    19261 tctgaaagga ttcccactcc catacctaga ccaagatacc aatcaaaatt cctaaagaat
    19321 tcgggaaagt ccttataagc tttcataccc caatgaaggg tagccattgc agctgcagaa
    19381 agatcgaaac ctttgccgaa atccatgcca gcaattccca tcacgccaag cgaaagagga
    19441 aagttcggaa ttgtgaattt ttgcaagata tattccattc caccgctgac tccaaaacct
    19501 ctccaaccaa aacctattcc acccaatgca tgtatctcgc ctacttcggc atgaacttca
    19561 tttgtaacgt tttgtgcaag caaagggcat aaaatagcaa cgatcataat acaagcaacc
    19621 atgaagattt tgcttctttt cacgtggtcc tcctatgcta cttaatctgg attagaaatg
    19681 ctaggctagt agcttataat ttatagtata tagcaatgac taactcttta tcaacatctc
    19741 tgttttcttt cctaattatg atattctgct ctaatgattc gttgcccatg gtgtggaagc
    19801 gacccagtct acattcaata tcacgacaaa gaatggggta gccctgttca tgatgatcaa
    19861 atccatttcg aattcttgct actcgaaacc caacaagcgg gcttgtcctg gcgaactgtt
    19921 ctcggtaagc gcgaggcata ccgaaatgca tttgcggatt ttgagccaca gatagttgcg
    19981 ggctttcgcg aggaagatat agctaacctg ctcgcaaatc ctaaactaat acgaaacaaa
    20041 cgaaaacttg aagcagctgt caaaaacgcg tgcgccttct gtgctattca gaagaaatat
    20101 ggctcctttg attcatggat ttggcatttt gttgaaggcc atccaattat caaccattgg
    20161 caagacattt ccgaaatacc cgtacagaca gagctttcag atctagttag caatgaaatg
    20221 aaaaaagctg gctttgcata tgttgggtcg atcacaatat actcccatct tcaagccatc
    20281 ggcattatca atgatcatct tataagttgc tttagacatg cagaaattgc gggcgcaaaa
    20341 taaatcctat cttatgcttt tccttttata taaaaaacct tatatttaaa acgcatggcc
    20401 tagccatgat atctaatcaa actctcaaaa ttagttagga ggaatgctat gttatcacaa
    20461 agaatgatcg atcgaatcaa tctacaaatc aataaagaaa tatactcagc ctatctctat
    20521 cttgctatgg ctgcaaagat gaatgagctt ggctactcag gcgtgggaaa atggctcacc
    20581 gtacagtatc atgaggaaat gtttcatgcc atgaaattcg cagaatacct acatgatcag
    20641 aatgccgaag tttcatatgc caagatcgac aaccctgaat tcaaagaaaa ggaaatcaag
    20701 cctctcttcc agcatgtgct tgcccatgag gagagcgtga ccgcaagtat ccgtgaaatc
    20761 atggaattgg ctattacaga gaaggattat gcgacgcaga tactcgctca gtggtatata
    20821 gacgagcagg tagaggaaga aaagaatgct acggagataa tccaaaagat agatctcctt
    20881 ggcaattctc atcagggact atatttgcta gattcggaac tagctaaacg agaatcttca
    20941 gtgccacttg attttaataa aatatgagca tcaagcgcag ggctaatgat ctatcatagc
    21001 ccttatacaa acaactcaga gatatcaaca gatgaacaaa actagaagag cccaatactt
    21061 aggagcctaa gctgctaaaa ggtagtgatt aattaaattt agcttgaaaa gctaacggca
    21121 agaagactgg ttttccgtag atttttccta tcagaaaggc atatggcaaa aggccaagcc
    21181 tagcatgggc ttttctcatt ctcagtagtt ttgtatgctc tatacatggc cgttcgttat
    21241 acgagtactc gagatgcccg aatcgatgtt agcgtcgctg aagcaattgt gcagggcatg
    21301 ccgccagatg gtggcctttt tgtacctcga aatgtcccta gattaagcgc ggatgctatt
    21361 cttgagccaa gactcagcta tgctgagctc gcatggctcg ttctatcgcc ctggttcgat
    21421 tggagggaga cagagctccg tccccttatc gaacaagcgt attcttcctc tggcgaaaaa
    21481 tcgaatttcg atacaccaga aatagtcttg ctatcaaatg caggttcgct aaactgcgaa
    21541 aacaaatact ctggcgaaaa acaatcgagc aagctttttc tgctcgagct ttttcatggc
    21601 aagacatgcg ctttcaaaga ccttgcacta agcctccttg gaggactatt gagcaaaagc
    21661 ctcgagaaat gtggcataga tgagcccgtt ctcgtactta cggcaacatc gggggatact
    21721 ggatccgctg ctctttcagg gcttggtgga caacaaggaa tccggatagc cgtgatatat
    21781 ccagcagatg ggacaagcga aatccaaagg ctccagatga cctctgtccc tcttgaagat
    21841 cgatgtgtta tcggactaaa gggaactttc gatgatgccc agcgaatagt aaaaaatatc
    21901 tttcagaacg caaaaaatct taggtctccc ttttttggaa ttcgactcag ctctgccaac
    21961 tccatcaaca tagggaggct tctgcctcaa atcgtatact atgtaaaagc atggcgggac
    22021 ttatattctt taggagcgat tgattatggc ggggcctttg atgtggttgt tccaagtggc
    22081 aatttcggtg atattctcgc tgcaagatat gccaaggcaa tggggcttcc tattcgtagg
    22141 ctggtctgtg cttccaattc gaatagaatt ctctatgatc tttttgcgac gggcatctat
    22201 gatagaagaa gatcattcaa caaaacgata agtccttcaa tggatatcct cgtctcaagc
    22261 aatctagagc gactccttta tcatgctttt tgcgaaaact ccgagctcgt atcgaggctg
    22321 atgcgacaac tcgaaaacga aggcttcttc ctaattccac agcaatttgc tgctaatata
    22381 tctgactttt gcgctggatg ggccgatgat cttcagacac tcgagagcat tcggcatatg
    22441 tgggaaattc acaatttgct tatcgatcct cacactgcgg ttgcttgcaa ggttgctcac
    22501 gaacttttca gtaattctca aaatcaccaa aaggatcgga caatcatgaa aactcaggta
    22561 gtcaatccta tcgtaatcgc cgcaactgcg agtcctttca agtttccagc atcctgctgt
    22621 gcggctttgc agagtggttt gccaaatccg ccgatctcca tctccaaaaa cgatctttcc
    22681 cgagcctttg agctttcgca ggctacgggt atctctgtcc ccaaacctat cgctgatctt
    22741 gtcaccgcta aaataaatca tcctataatt gtggaggaat cagccctaga atcagttctg
    22801 gctgattttg ctcataagac tgctaactca catgcctgca gaaaattaac gatatgaggt
    22861 tctcatgata gaaatcgaag tccctgctag ctgcgcaaat cttggcccag gtttcgattg
    22921 ccttggaata gcgttgtctc tttcgaatat cgtgcgtttc gaaccagctg agaaaactca
    22981 gataaaaggc tgccccatgg aatgggctga tgaggataac ctcttcctgc gctctttgcg
    23041 ccatagctgt gagctattgc aaacctctgt ccccaagatc tcggccgaaa tcattacggg
    23101 tattcccccc gcaagaggtc ttggctcaag tgcatctctt gctgtagctg gtgctgctgc
    23161 ggcattgctt ctttcaggaa agacagccaa gagtctcaaa gatctagcta atactgcagt
    23221 tggtacagta accagcctgg ggacagaggt tgatatgatg ggcgacgcgg tcggtgacac
    23281 ggagacggag tttaacctaa gagcttatac ggggacagag gttgatcgga catgtctgac
    23341 acgctttctg cacgaagaag aaaatcagcg ttttcttctt agagcagcaa ccgatatcga
    23401 agggcatgcc gacaatgcag ctccagctat ctatggtggt tttacagctg cggcgctcac
    23461 cgacaagggt atagtgatca gccagaatcc agcccctgag cattggcagt tcatagcctg
    23521 catccccgat ttcattctcg aaacaagctt agcacgcaag gctcttccag atttctattc
    23581 aagacaagat gtggttcacg gtatttctca tgcagtgctt gtagctctag cttttcttaa
    23641 ggggaatctc gatcttttaa accttgcatg ttcggatcga atccacgaac catatcgtcg
    23701 acctcttatt cctgatttcg aaatagtgga aactgcatgc ttcgaacaag gtgcaaaaat
    23761 ggtctgtatc tcaggctctg gccctactat catggcagtc tttgaaacct ctgtccccga
    23821 tgtcgcatca agcactacag atatcctgaa tgagacgatt gagacacaaa ttgcaaaaag
    23881 cgctactcac aaatggatag tattgccttt aagagtcgac aatctgggaa ttagggctcg
    23941 aaaaatttga aaaggtgttt ctgggaggga ttctcataat gaatactcag aatatgataa
    24001 aaagccagga caagcgtttt tggctaagac ctcaattctt accaaccttc tactataata
    24061 ttccttcttc ttttggagaa atcctgctta tctggcatga tacggatact ccaatgattc
    24121 attgtattta tcttcctaaa aatggggttt ctcgaatagc tcaagctaag actgatctca
    24181 atctattgga accatggaga aaagaagttc cgctgcctca acatattgaa cagattttgc
    24241 aattgctttt gaaaatgctt tcaggccatc cagtacaggt accccttcag atcattgagc
    24301 attctctttc taatctcaaa ctacccgaat ttcaacaaag ggtgctcttg accgaagcca
    24361 ccatcccctt tggttatata agcagttata aggaactcgc gaaggcttgt ggaagacagc
    24421 tctcttttag agcggtagca agctcactct cacataatcc acttcccctt ctaattccct
    24481 gccatcgcat agtggcatca aatgggaatc ttgcaggata tcaaggggga attgaaatga
    24541 agtgttcact tctcgaaaac gaatccattc caatccgctc gaacaaggtg attatggaaa
    24601 aggcaaagtt ttgggattat tcctcaaatt catgaatgga ctgttgctct ttgtctgtca
    24661 aagctgcctt gccttcttta gcaccctcct tagggcgttc gaatatttct gataattaat
    24721 ctatcaaacc tccgtcccta aaaagatcac acagagatcg cggatcttta gacacgattc
    24781 cctttttgac ctcagagatt tcttataaac ctaccatgga tcttattaat ccttttgaaa
    24841 cctctgtccc cacatacccc cgcatacgcc tacataaccc atttacgcgc attaagtaca
    24901 cgcttccaac aacatcctta aatatttagg gttttcctcg ctataactag acaccgctct
    24961 tcttcaagga atggaaccca cactggagta atcttggcat gagctgccat attgctaagc
    25021 acaggatcat ccaagagtcc tgtcaattct agctttgcat gcataatgcg ccctttatag
    25081 gcgcataagg ctccgcccaa tttaatcttt ttccaaatgg ttcgaaataa ttttttgtca
    25141 gtgaatggcc tcaatgccct gaatatggca cattccaaat tctcgtcttc atcttctaca
    25201 gtattttgat gaatttctac attttcaagt cctaatatac aaataacgtt ctccagaaaa
    25261 cgggttcttt tctctagtct ttcaatcaaa agaaaggatc tttgaggaaa cgcaatggct
    25321 agtggaatgc ctgggaatcc cgctcctgtc ccaatatcag cgatgcgcaa tttattatta
    25381 tcggagaatt gcttgtccat ctcactgaat agatcctcca atatccgcca aggaccaagg
    25441 ctgtccaaga catgcttaat aatcaaatct tgtcctttcg ccttgacgaa gccataaaca
    25501 ggattccatt gctccagttc atcgacataa cgcaataatt tttcaagaac cccactggaa
    25561 atatcgaagt cgagtgcagc caagccccgc gctaataatt gttgttgatc catctgattt
    25621 tattatatca ttagaagctt cgcgcataaa tcgatgaaat acccaatatt catagccata
    25681 gttcagcaag ataatcttaa tcgatccatt tttagtcctt attcctattc ttttttctgg
    25741 aggattgaca gcaagtcccg caatttggga tactaaaaac ccatgagaaa actattattc
    25801 gtaacgggag gcgtgtgctc atccctcgga aaagggattg cagcgtcttc catcggtgct
    25861 ctcatggaag ctcgcggttt taatgtgcag atggttaaaa tcgaccccta cctaaatgtc
    25921 gatgccggca caatgagccc ttatcaacat ggcgaagtct atgtcactga tgatggggct
    25981 gaaaccgatc tagacttagg caattatgga agattcacaa ctgcaaagct gagcagatca
    26041 aactccatta caacaggtca agtctacaaa aaggtcatcg aaaaagagag agccggagac
    26101 tatctcggcc gcacagtaca ggtcgttcca catatcactg acgaaatcaa agagcgcata
    26161 ctagccgttg caagcaatcc tgatgtagac atgacgatcg tggagattgg aggtacggtg
    26221 ggcgatatcg aatcggtgcc atttcttgaa gcctgccgac agctcattca cgaattaggc
    26281 aaacagaatg caatttcaat tcatgtcacc ctggtcccag ctgtctccag cggcgagatc
    26341 aagacaaaac caacacagca tgccgtcaag gaattgcagg aaataggaat acagcccgat
    26401 gcacttatcc ttcgctcgtc aaaacccctg agcgatgaac tacgccgcaa gatctcgctc
    26461 ttcaccaatg tagaagaaga aggagtcatc agtggttacg acgcaacgac aatttacgaa
    26521 gtacctctca tctattacag ccagaggctc gatacattcc tgctacgaaa aatgaatgtg
    26581 gaaagccgcc acgcggagct gaacaagtgg caaacggtcg ttcaggcaat aacaaagccc
    26641 aatcgtaaag tcaagatagc agtagttggc aaatacatgg atctgcaaga cgcttataaa
    26701 tcgatatggg aagccttgat ccatggaggt atcgcatacc atgccggcat agatatagaa
    26761 agaatcgatt cctcgcgcct cgaagacccg cgagcagacc tagatgctat attcaaagac
    26821 atccatggaa ttttgatacc agggggtttt ggtgagcgag gaatagaagg catgatatgc
    26881 gctgctagat atgcaagaga aaagaacatt ccatattttg gcatttgtct aggtatgcag
    26941 atcatggtca tcgaatacgc gcgttcaata ctaggatggc aagatgctca ttcaactgag
    27001 ttcaataaat ccactcaaca tcctgttgtc tgcttgcttg aggaacaaaa gaaaatgaaa
    27061 aattatggcg gaaccatgag actcggcgca tatgatgcgg ttacaaaagt tggttcaaac
    27121 attcgagctg cttatggtgt cgaattgatc agcgagcgcc atcgccatcg atatgaggtc
    27181 aatagtgata ttcgtctcga actcgaagcc catgatctac gcataagcgc gacaacagtg
    27241 gatggagagc taatcgaagc atgcgaatgg ccaaatcacc cgtggtcagt aggcgttcaa
    27301 tttcatccag aatttaaatc gaggcctaca gctcctcatc cactatttag agaatttatc
    27361 ggagcagcct tgagcctctc tagattatag tacaaactaa agagtgcttt gcccatcaag
    27421 gagcaaggca gggatatagg tgttttagga tatgggtgtt tatagaagag aagaatggct
    27481 gattaaatgc attttttctc tttgtctatt attcgccttg attgctctat tgtcttcctg
    27541 tagtcaagta actgaagaga attcccttac tgctaaaaaa gatattccag atgctcaatt
    27601 ttatgatctc ataagaaaag aatatcggga aggaaagcta tccatgcaat tctccgcaaa
    27661 acatgcttcc tggtatgaaa cagataggcg cttggaggtt gaagagcttg ctttcatcac
    27721 ctacaatggc gatgatggct ctatatcagc ttatggcgat gcgcaacacg ctatattata
    27781 tgaaattagt ggagacgcag agttctcagg ccatgtgcat ctgcagagtg ctgaaggcga
    27841 tgcttgcttt gaagcttttg aacttagtta tagaagaagc cttgatgtat tcgagacacc
    27901 ctttgacgcc gaagttctga taaaaatagg caatcagctc cttcttgctg gcaatggcct
    27961 tcttttcgat gttaagcaac aatactatca tattgagaaa aatgttcatg gttcagtaaa
    28021 tcaataatat gaggtagaat gtcggcatga aatcgaagat ttttgcattg attctcagct
    28081 ttatgatgat attcgtacca ttcagtattg gctttgctca aacaaacccg cccaatgtgc
    28141 agcctagccc tgaaccgcca atgcaaggtc aacagactca gccttcgagc ggcaaaaagc
    28201 caatcgtctt tagcgctaat actgtccagg gagttattgc aaaaggcaaa gagaatacca
    28261 ttcttatggg atccgtacat gttacgacag gaagcttgac cattaccgca gatcgcatag
    28321 atcttagcgg cgaagattat gtcaatgtag tctgcttggg caatgtaacc gtactggaca
    28381 cggaaaaagg attctctcta gtcgctgaaa agctcaacta ccatcgagat accgagatag
    28441 gcattgcgca gagaaaggtt tatgtaaatg atacaaaaaa caacaccatc atagaagctg
    28501 aatggttaag attcgatcaa aaacaatcaa tctttgaagc agcggtcaca gtgcaggtac
    28561 gcaaggaaga tatgtcgata agttcagaat atgcattgta taatcgcgat acagaggaaa
    28621 tatggcttca tggtggtgct attgcagtga ccgaagatgg cgtccttaaa ggcgatgtaa
    28681 tttcttcttc gtctgactgg tccaaactca agatctctgg tgaagtctca gggactatca
    28741 ccccaaagga gaccgccaca tctcaatgac cgatgcaata ctcgaagtat tcaacttaag
    28801 aaaatccttt ggtcctgtac tcgctgtctc tgatgtcagt ttttccatga aggaaggtga
    28861 gataattggt ttacttggcc caaacggagc tggaaagaca acagtattct atataatcgc
    28921 aggatttcta aaaccaaatt ctggctccgt acgtctcaat ggagtgatta ttaataatct
    28981 ccccatgtat aggcgagcaa tacttggaat ctcatatctc ccccaggagt cgtctatttt
    29041 tcgcaggatg accgtcgaag aaaatatcct tgcggtgctc gaagtccgta gtggattatc
    29101 ctctgctgca agaaaggaaa aagcctattc tcttatggat gaatttggga ttgtgcatct
    29161 tgcgaaacag ccggcctaca ctctgtctgg aggcgaacgc cgaagaactg aaatagcaag
    29221 ggctttagca gtagagccga aatttcttct cctcgatgaa ccatttactg ggatagaccc
    29281 tattgccata cgcgaaataa agtcccttat acataatctc tctaataaag gaataggtat
    29341 tctcttgact gatcataatg ttcgtgacac gcttgctatt acat
//
LOCUS       DAOOWY010000013        29383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1592_length_29383_cov_10.183954, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000013 DAOOWY010000000
VERSION     DAOOWY010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 29383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29383
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1592_length_29383_cov_10.183954"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1403)
                     /locus_tag="PK105_02105"
     CDS             complement(126..1403)
                     /locus_tag="PK105_02105"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU60530.1"
                     /translation="MIFAVIAFAVFLFMGVPVSFSLGSSSLIYLLVEGKPIGLVAQRM
                     FTGIDSFPFMAIPFFVLAGELMNRAGISKRLIDLADVFVGRLRGGLGHVNVLTSMFFA
                     GITGSAVADTSSEGPIIIPAMVAAGYDSDYAAAITCASSVIGPIIPPSIPFVTYSLVS
                     ATSVAALFMSGLIPGILLGISQMIANAIISKKRNYPRSERWPSPKEVARAIGRGIIPL
                     FMPLIILGGIIGGIFTPTEAAAVAVLYALLISLLVFKTIGWKDIRGALIETAKTTGVV
                     FLLIGCSNVFNWALVVEQIPQSIALFIGNTITNKYLLLLLINIFLLIVGCFMEGTAAI
                     IILSPLLLQITKPFGISPIMLGTILVLNLMIGLITPPVGLCLFIACNIAKRPIEAVSK
                     ALAPFLIASIIALLLVTYFEPLSMWLPRLLGYA"
     gene            complement(1400..1909)
                     /locus_tag="PK105_02110"
     CDS             complement(1400..1909)
                     /locus_tag="PK105_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOU60531.1"
                     /translation="MKEQSLFMKTVGKLESAVRWIVVVLLIIMVVDIITAVFFRYVLQ
                     NSIFWAEELSRYLMIWAGMLGAGLAMKDDAHIGIDFVVRALPNSIQFIAKVIAKLVVE
                     IFLIVVFIESIDYLKTLSIQRSAALEIPMALPYLSVTAGMVFMAIENILGIIRLFDDE
                     NQRRQEINK"
     gene            complement(2048..3082)
                     /gene="dctP"
                     /locus_tag="PK105_02115"
     CDS             complement(2048..3082)
                     /gene="dctP"
                     /locus_tag="PK105_02115"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOU60532.1"
                     /translation="MKKLGLMVLLVCIVLSGAFGQGKVYELKLAHADPTDPAVSRKQA
                     QAIAFASMVNSRSGGRINVKIFGGGALGAEREYIEGIKAGTIEAGLASGPVADFFPPA
                     AITDIPYLFPTSKVAWDVMDGAFGDKLRDMFLKQTGMRCLAFGEVGFRHFTNSVRPIK
                     SPSDLKGMKIRVMETPVYVNMLRAAGANPTPIAWTETYTALQTHVVDGQENPIASIIM
                     AKLYEVQKYITLDGHSYGVDWFIINEKFFQSLPPDLQYIVVDTAQTVAAVGRGFITLQ
                     NAQGLQTLIDAGMQVYSPTDAEMQQFKKVMQPAVIDWLKTKVDPALINELLKAVDDAI
                     VAQKAKEGLK"
     gene            complement(3135..4097)
                     /gene="iolN"
                     /locus_tag="PK105_02120"
     CDS             complement(3135..4097)
                     /gene="iolN"
                     /locus_tag="PK105_02120"
                     /EC_number="3.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF041098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-scyllo-inosose hydrolase"
                     /protein_id="HOU60533.1"
                     /translation="MSGWKFPQEGQMDKPSKIYFQTMTKYDVEERLKTNDLILIPIGS
                     TENHGNAGPYGEDTFIVTRIAELVAKQVGCTVAMPMWYGSHPFHHIGQPGTVPLSDDV
                     FTAHIRAMISGFWNAGFRKQIFLSMHGQEYALPSAIQEWGKKYQVPAIVIFLDLPRIM
                     GTDLKDKEHGGPFETPFQHADEAETSISMALFPELCQPEHAENTSLKGFLPPGHVDRG
                     GDIYGNPIPGHCIIGGTGIECVTFPEGVLGKAKLADPNKAKKAIENVLDYMVRLHNDI
                     MERFPVGKLPEIKLMTQRDEKEIELVLKGPLNGGKHIYTIAWPS"
     gene            complement(4119..4487)
                     /locus_tag="PK105_02125"
     CDS             complement(4119..4487)
                     /locus_tag="PK105_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU60534.1"
                     /translation="MSELKVIDINKVEPEKKTPPRSSWILISEKTVGAQNLALGVNET
                     EPGSMVPEHTHETEEEVMFFLEGEGKFITQDQEIDLKPGICVYNPPGAPHKIVNTGTT
                     KLKFVWVYAPQLKSHRIPNN"
     gene            complement(4526..5272)
                     /locus_tag="PK105_02130"
     CDS             complement(4526..5272)
                     /locus_tag="PK105_02130"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU60535.1"
                     /translation="MELENTIAIITGSGNGLGMGIAKKMFAEGASLALWDIDFESVKR
                     LAFELDHSGQRVLPLKVDITDESGVQSAAATTVDKFGHIDVLVNNAGISRHKPLEETT
                     LEIWDAVIKVNLTGTFICCKAVAPVMKKQEFGKIVNIASLGGRTGRPGVGVNYAASKG
                     GVIGLTKLLAKELGPSNIYVNAICPGPILTDQTKQYPREVFDTWNAGRAVPKDGLPED
                     IGDAVVFLASRRSNWITGVSLDVNGGIFIA"
     gene            complement(5312..6346)
                     /locus_tag="PK105_02135"
     CDS             complement(5312..6346)
                     /locus_tag="PK105_02135"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOU60536.1"
                     /translation="MKALVYYGPHDIRYQDVPTPRPKEGEVLVHVRAVSICGSDLAAY
                     KAVSSMRSPGLIMGHEFSGEIVQLGEGVDNLKVGNRVGVITNIYCGKCSDCLEGRTNV
                     CDHRYIIGTTMKAGSYNGAMSEFVVAPAEKIMLLPDNVSFDEAALVEPLSISLRATKH
                     VAHEVPSRAVAVLGVGPIGLMAIMCMKNLGSAKIVAIDLLQNRLELARKCGATDTILS
                     GQDVPALIRQMTDNVGIDVVFDAAGVPSTVNTAIEIVRNGGSILMVGMASPTLEIAYK
                     HAVSKELKLQASYMYTTEMKEGLQMIADAKLNVAQMITSRHPLSEGPKVFEELASGNT
                     KEVKVILFPE"
     gene            complement(6367..7176)
                     /locus_tag="PK105_02140"
     CDS             complement(6367..7176)
                     /locus_tag="PK105_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOU60537.1"
                     /translation="MKMRLGCFGYIKDLDTIAAAGFDCAELHIREIMAMSEGEFKKAK
                     KALRDCGIPAEVFDNPIPLDCCISSPSFYLDHFRDFLEKSADRIAEMGARYIVFGNGK
                     ARSLPSEGDISIAASKFDDFFISLLEIAAKRNITVLIEPLAKSLSNIVNSLPEAIEFI
                     KKYEKHNLKTLLDYRWMLEDGWPLTDIEDYELYIKHVHLDNPLSPFPTRVVPRMNDGF
                     DYAPFLNTLKRIAYKEIISIEACVFQDFAKEIREGLELFKANNIEPYHSIS"
     gene            complement(7211..8269)
                     /locus_tag="PK105_02145"
     CDS             complement(7211..8269)
                     /locus_tag="PK105_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOU60538.1"
                     /translation="MKIFYRDLQNAACEILRLVGESKENAQIASEIMVINDLRGISTH
                     GTNLLRMIAKRCEAKMLSLPTRVVVVSEDMATLVLDGGDGLGQVAGYKMMNAAIEKAH
                     LYGLGMALLRDTNNVGSLGYYVHKAAMQNMAAIMMTNGNPSMAPYGGAEPFLGSNPIA
                     FAVPTSSEMPVMLDMSSSVVARGKIRMAALKNENIPLDWALDSEGNPTSDPQKALKGC
                     LMPVGGPKGSGLAIIVDIFAGILSGSSYGRNLKSFHELEGKTGVGACFIAIDISRFMA
                     VDKFRNLVGEYARALKSCELREGFEEILLPGEIELRKEQESRENGIDMPVEVINDLNE
                     LLRKAGANFLLRPMDQCR"
     gene            complement(8274..8948)
                     /locus_tag="PK105_02150"
     CDS             complement(8274..8948)
                     /locus_tag="PK105_02150"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HOU60539.1"
                     /translation="MIHVLKIAERPDQNIIESFRKLAVATIYEASGKKGFIDCAIKPL
                     AKGMRICGPAFTVQCVPGDNLMLHKALQTAKAGDVLVASMGDGYFYGYLGSLMGTAAI
                     TAKLGGLAIDGCVRDTEELIEMDFPVFCRGACIRGTAKNGLGTINYPLNFGGATIYPG
                     DLIMGDDDGMVVVRRQDLADVLQKSLDRVANEKKKSEQLQRGISSMELNKLEDVCIAL
                     GMKEEE"
     gene            complement(8962..9618)
                     /locus_tag="PK105_02155"
     CDS             complement(8962..9618)
                     /locus_tag="PK105_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU60540.1"
                     /translation="MIQKDQIDATNKKNMAYEIIKQRIINNEYKPLDTLTEQLICSEL
                     GISKTPVREAFKELEKDGFVKIIPSIGCLVSSIGLDYIHEIFEIREILECAAARIVAE
                     RGDKTIFEEILKNHESFELAEKKNIKGSVVSGFEIHTAIFETVGNSRLLDFYKNIQNH
                     IIRIRLFFIYQFDMEKTERMNAEHKMILKTIIAGNSEAAEKAMREHLRNSQERLKSLL
                     "
     gene            complement(9798..10889)
                     /locus_tag="PK105_02160"
     CDS             complement(9798..10889)
                     /locus_tag="PK105_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOU60541.1"
                     /translation="MSQKIRIDRVRLEQFCTDVFIKLGLPQDEAQDSAEILVAADARG
                     IESHGVGRLWRYKNGLQKGIMKGGILPTTLQETPISLVLDANGAMGLSLSKRTMAKII
                     EKARVAGAAFSSVRNSNHFGIAGFYSEMAAREDMIGIGMTNTAALGVPTFGRKAMFGT
                     NPIAFSIPAHDGRIFTLDMATTVVTRGKIEVYDRENKPLPSGWAVDTRGKGTSDAHRL
                     LEDMLYQRGGGLLPLGGEGELLSGYKGYGLAEVVDILTAILSGGVFGQAVIDSQATSA
                     RVCHFFGALRLDLFRDAEAIKSDMDRLLTDLEMAEPAEGCERVYYAGLKEHEAEAESA
                     HFGVPISEKVALQLRQIAQELGVEPPIIS"
     gene            complement(10906..11826)
                     /locus_tag="PK105_02165"
     CDS             complement(10906..11826)
                     /locus_tag="PK105_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HOU60542.1"
                     /translation="MRDVRHFQNCALPKSVEVIQGKGGFPLLHILNRYAECSLYLYGA
                     HVVSFKPQDQTELLWLSPYSRFSKGTPIRGGIPICFPWFSKHRTMDYLPLHGFVRTEF
                     WNIESAEDLTDGRTKIELSTESEEPLPDSWPYHFRLKIIITVGEKLEIALIIQNLENL
                     PIICEDGFHTYFKVNNPHGCQVRGLDGIEYIDRTKGDIRERQNGLACFEGEIVHAFMH
                     APQRLELVDLVDNRRICIEQENMDSIVLWNPGKDVGAVNSEIFSAWDQFVCVESTNCL
                     DCQLSIPPLGSHCSILRLSSISFDKEEKRR"
     gene            11900..12898
                     /locus_tag="PK105_02170"
     CDS             11900..12898
                     /locus_tag="PK105_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HOU60543.1"
                     /translation="MKKKSMVLVVFFLIAAIFLVSAQTEKKYVLKFGHVLTTQDPYHE
                     AFLKMAEAVSARTNGNLKVEVYPSAQLGVEEDILEQIRQGANIGWNTDAARLGMYVPA
                     IAVINAPYFVDSLEEVKRLNTIPSVSAWKAELERTQGIKILSFMWVQGFRQMITNKPI
                     RKPADLSGLRIRTPPAPIWQESVRAIGAVPVAMAYGEMYSALQTRAIDGLELSFTATA
                     SGKFQEVAKYACETKHILLINFEVVSSKWFNSLPVEYQKILQEECDKAGLEVSRRYLE
                     EIDPKNIQVLKAGGMTIIPASEIDMAAFKAAGEKAYQKLNLVAVRDQVYKELGKTK"
     gene            12976..13446
                     /locus_tag="PK105_02175"
     CDS             12976..13446
                     /locus_tag="PK105_02175"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOU60544.1"
                     /translation="MRKIYHFFGKAEVLVSAVCFTVSLLLIFSGAIARTLRHPLNWSQ
                     DLSLFLFAWGVFLSADAAMRADKLVNINLLVARLSENMQKILATVVYVIIFIFLIFLS
                     YNGIKLSIFSHRRVFQGIPGFSYSWVMVSIPVGSVLQMITIILKIRDLYKREEH"
     gene            13448..14722
                     /locus_tag="PK105_02180"
     CDS             13448..14722
                     /locus_tag="PK105_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU60545.1"
                     /translation="MLIVGILLLAFLAIGMPIAYAIGISGFAFFLQHPELPMTIIAQL
                     PISQTQNFTLLAVPLFILAGNLMNASGITNRLIKLSTVLTGHMRGGLAQVNVMLSTLM
                     GGVSGSAIADAAMEARILCPAMIERGYSPGFTANCTAWTGLITATIPPGVGIILYGVT
                     GEVSIGRLFAAGIFVGIFLTAVYMFTVWIISGRKGYKAERARASFQECMQAIVENIWA
                     LLFPILLLVGLRIGVFTPSEVGSFACVYAVFVGIFAYKELSLKKLIQTLGESVMDVGG
                     IMFIIALSGIFGYGIPFDRIPDLISNAILGFSTNRYIVLFIILFILIILGMFIDGSVV
                     ILLFTPIFLPLAAQLGWDPVHFGILFCTIITMGNMTPPVGLAMSAVCTVLNVPISEYI
                     KEMWPWFWATVLAVSVLVFFPGLVLALPRLLF"
     gene            14736..15476
                     /locus_tag="PK105_02185"
     CDS             14736..15476
                     /locus_tag="PK105_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU60546.1"
                     /translation="MELQGKTAIVTGSARGIGFEIAMVLAREGAFVVLVDQTGTQDAA
                     ISLQEKHYGAMAISLDITDLDAVNNAVKTIVSAKGRIDILVNNAGIIARGSIIDLSRE
                     QWLRVMDVNVNGNFYFCKAILPYMIRQHSGNIVNITSIAGKMGDITAAPVYGASKGAI
                     NAFTKSLARQLAEYGIRVNAVAPHAIETSMSSEWTIEKREKVIDAIPLKRMGKPEEVA
                     EAVLFLASERSSFITGEILNVNGGMLMD"
     gene            complement(15488..16192)
                     /locus_tag="PK105_02190"
     CDS             complement(15488..16192)
                     /locus_tag="PK105_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU60547.1"
                     /translation="MEESDNIIIEKRSLAEQVYLYLCNSISNGKMKYGETINTRRLAE
                     ELNISMMPVREALKRLETDGIVEIKPRSMCLLRTPTRETILDAIAAREMLEIYSVKSI
                     YASIENSRLDVLRKIIEEMKMAISEDPIDLRKYIKHDWQFHKELCNLVDNQFVNKFYK
                     ELNIHLNMSYVYDIGIEPNVSQTFRDHIDLVNALAAHSSEALKIIERHLRTNRQNILS
                     GRFFSSEKEGGSKRAV"
     gene            16494..17885
                     /locus_tag="PK105_02195"
     CDS             16494..17885
                     /locus_tag="PK105_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU60548.1"
                     /translation="MKLAFLHQSTELRRDYILNGPLSSTLMLLAAPTLMLGVVQALMP
                     LMDGLFINNIAGTLVASSVTFSEPVINMTTALAQGLSVAAMAIIGQLNGRGDLKESKR
                     TSTQILVMGSMLGCISAPFLVLAAKIISSRVNPGISHNVFLYLSLYALVLPFSFMESI
                     YNGIKNANGMPEAPFIRMVLMLILKIAFNFLYIYEFRMGIVGCVLSSLSANIIITLWM
                     FWELFLKKGPDRLELKGFKFDPVAAKQLFRIGAPAMANTFILNLGFFLINTEVEKYGP
                     VVLNGQGIANNITQVAFNVPSAFGSAVTTMVSMNIGAENPRKARESMVHGTTLSALTA
                     AILIAIIVPLSSRLTVLFTRRQDVLEIANRALHIYTYSVIGFGITTTIQGAFIGLGRT
                     RVPLVLGILRIWLLRYAFILVTEKYLQYYAVFWGNLFSNYASAIIAIILISRTKWESA
                     IGKSMQLEVASAK"
     gene            complement(17951..18742)
                     /locus_tag="PK105_02200"
     CDS             complement(17951..18742)
                     /locus_tag="PK105_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU60549.1"
                     /translation="MVRIEDVVVSFDLGGGQEHTVLNKLSLEVKQGQTVSIIGSNGAG
                     KSTLLNSIAGSIPLHAGHIHLNGEDVTSWAEWERARYLGRVRQDPMAGTAGEMTVIDN
                     LALAARKGPRRFRIATPPRFAREMERKLAELGMGLESRLRENVSRLSGGQRQALTLLM
                     AVISHPTILLLDEHTAALDPANAEIVDALTRRFINEYDLTALIITHDMKRALEQASRV
                     IMMHEGEIIADLSGPEKEKMNVAALVRLFKKVRGSEYAEDRDLLI"
     gene            complement(18773..19678)
                     /locus_tag="PK105_02205"
     CDS             complement(18773..19678)
                     /locus_tag="PK105_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60550.1"
                     /translation="MVEGILVEGLIYGILAFGVFVSFRILDFPDLTVEGSFPAGAAAG
                     AVVASAFNLSSNAVLHGLAVPAGMLAGFVVGGIAGFATAAVYHKLRIPPLLAGIVTMT
                     GFYSINLRILGGKPNYPLITNNPMLKLARSFLGGALPSEWSLFASCVLVCLVLFGLLD
                     LFFHTEIGIAMGALGDNENAVIQAGIQPNRLRTWGIMLANALPGLSGAMAAAYQGFAD
                     VNLGQGVVASGLATVMLGELIIRSQFISVQLLRVLIGSILFRALMYAARSWGYVAGIT
                     PNDLRLLTALLIILSVALSRIKRKH"
     gene            complement(19682..20674)
                     /locus_tag="PK105_02210"
     CDS             complement(19682..20674)
                     /locus_tag="PK105_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU60551.1"
                     /translation="MSNRFAKLLYAIGCAGILLLLSCAPKGAKVVGVAKFVSHPALDA
                     LEKGILDELSASKADYKVDLQNANADMNTAAQIAQHFKQEKVALAVGIATPMAQALAN
                     QIKDRPVIYSAVTDPVAADLVESMDKGGPNVTGTSDMTPVREQLDLLCSLKPSIKRIG
                     NIYASGEANSVALAEIVREYCQEHGLEYVESTITNSSEARQALLSIAGRIDGLYLGND
                     NTVFSALSGIAEVGIDKKIPIVTADPSSAETIPVLAALGYDYYRMGLATGKIVVRILN
                     GEKTSDIPAFLSKENEDMTFVLNLDTARKIGISVDQSIIDRAKVVISDDQLVRK"
     gene            21063..22181
                     /locus_tag="PK105_02215"
     CDS             21063..22181
                     /locus_tag="PK105_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU60552.1"
                     /translation="MKQRKMLFAAIVSLVLFLGAQAVFAAPTIKIGVAGAHSGDLASY
                     GLPTVNAAKLVVKEYNDKGGINGQKVELIIEDDQCKPEIATNVAAKLVSTKVAAVIGH
                     ICSGATKAALGIYKDSRLVCISPSATTPFLTQSGEYPNFFRTIAPDDAQARLATDFLV
                     KDLKVKKIAVLHDKGDYGKGFAESVREFAEEMGVKVALFEGINPGAPDYSAVINKVGN
                     AKVDAVVWGGYHPEASKLVQQMKEKGINVAFISEDGIKDNTFIEVAGKYAEGVYATGP
                     TDTSANPMANKAIEAHKKEFGSEPGAFFLNAYASAQALLNAIEKAKSTDYDKIVAALQ
                     SEYVDTVLGRITFDKKGDIVGYGNSLFQVKNGKFIVLK"
     gene            22271..23173
                     /locus_tag="PK105_02220"
     CDS             22271..23173
                     /locus_tag="PK105_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967890.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HOU60553.1"
                     /translation="MEYFIKLFLSGTAKGSIYALMALGYTMVYGIIQLINFAHGEIYM
                     IGGFTALILGGFFFSKGMPVSLVLVFSILLSIIYAAAFGFTIEKIAYKPLRGKPRLSA
                     LISAIGVSIILQNFVLLSQTEKFLPFPNYLPEFSWLLPYRSYINSTQLIILITTAIIM
                     VLLTLLIKFTRMGKAMRATAQDMHMAQLVGVDINQVISLTFVIGSSLAAIGGVLICSY
                     MGQINYYIGFIAGIKAFVAAVLGGIGSIPGAVLGSFVLGWTESFGTGYVSSDYEDAFA
                     FLILIIILLIKPDGILGRSRRQKV"
     gene            23177..24448
                     /locus_tag="PK105_02225"
     CDS             23177..24448
                     /locus_tag="PK105_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU60554.1"
                     /translation="MKARSILKDIGKSLIIAVWFMILFFPIMVMKVNVTRGVAHINFR
                     WNVLPLIGIGAFVLSFVWTKALEWNERRTSKKKIENGLINNRALNGRYLKNPALRPLI
                     LLLVFGFTVAFPFLFGMYHTNVMITAFIYVILGLGLNIIVGLGGLLNLGYAAFFGIGA
                     YTYGLIWKYIGPSFIAAGLNPGWLFWISLPLAGIAATIFGVILSLPVLRLRGDYLAIV
                     TLAFGEIFHMVMQNAGDLTGGATGISLIPRPWFLGQKLQPSEAATYIYFIALVFVMIT
                     IFVVRRIEDSKVGRALEAMREDEVACEAMGIDMVKNKLITFAVGTFWAGIAGVLMASQ
                     TTYINPDSFTLWESIMILMAVVIGGTGSIPGVIAGAILLKLLPEYFRPLAQYRMLIYG
                     IAMILVIIFKSDGLIPRKRKQYTFTEKELAK"
     gene            24445..25245
                     /locus_tag="PK105_02230"
     CDS             24445..25245
                     /locus_tag="PK105_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60555.1"
                     /translation="MSTEKLQYYNEILEIDNLVMAFGGLRAIDGVSMHIDEGEIAALI
                     GPNGAGKTTIFNCITGVHKPTEGKVKIRNRDGRVEEIQGLKPNVINCKGLARTFQNIR
                     LFNNMTVLENVMIGRHNSLKAGVFAAIIRNAITREEEQRVIEESYTILKKLKLDTYVN
                     ETAKNLPYGEQRRLEIARALATDPFLLLLDEPVAGMNAQETKELEETINIIRDQEHIT
                     ILLIEHDMSLVMDVSERIYVLDYGRLIAEGNPHEIKKNPDVIKAYLGE"
     gene            25247..25960
                     /locus_tag="PK105_02235"
     CDS             25247..25960
                     /locus_tag="PK105_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60556.1"
                     /translation="MALLELKDVRTFYGNIQALKGVSISVEEGEIVTLIGANGAGKTT
                     TLMSICGIVPIRSGEILLDCQDISRMSPHKIVQRGVSQVPEGRRIFPQLTVSENLDMG
                     AFLRTDREGIKRNMEEVFTIFPRLAERRNQLGGTLSGGEQQMLAISRALMANPRLLLL
                     DEPSLGLAPLIVQNIFEVIQKINKERNTTILLVEQNANMALKIANKGYVMQNGLIKIT
                     DTAADLLKNQEVRRAYLGL"
     gene            26034..26678
                     /locus_tag="PK105_02240"
     CDS             26034..26678
                     /locus_tag="PK105_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOU60557.1"
                     /translation="MKVGQRMTRNPVTITPDTTVPEAQAIMRREKIRRLPVLDKNGKL
                     VGIVSALDLTHASPSPATTLDVWEMHYLLSKLTVDKVMTRDVITITEDLPIEEAARIM
                     DDNQISGIPVMRGEILVGIITESDIFRLFIELFGARHKGIRLTLLLPERKGELAKVSN
                     AITKCGGNIVSFATFEGEDPTNAYCTIKVTGVEEKTLIEAITPVVQKVVDVRET"
     gene            complement(26714..27142)
                     /locus_tag="PK105_02245"
     CDS             complement(26714..27142)
                     /locus_tag="PK105_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOU60558.1"
                     /translation="MAVRKGYIRPSPIDADIGAQLELLRKAGVESIFEEKPLSKEADR
                     PELRRALDSLCNGDTLFVASLDRLARTITELFYILEEIRRRGANLESLKEQLKIEDDF
                     IRHLGIIIDFERISLLEWQRGKIEAKKSWRERGAGSKRQR"
     gene            27476..27952
                     /locus_tag="PK105_02250"
     CDS             27476..27952
                     /locus_tag="PK105_02250"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HOU60559.1"
                     /translation="MKIALVLLGVPVFILSVLGLFWLANNPANPSYAYMLYPILVGIY
                     LSTIPFYIALYQAFKILINIDKDNALSKQCARALKNIKLCAIAVSILYTGILPLVFFI
                     AQKDDAPGLIVFATIPIFISLVIAIFAAVFQALFGNIMDMSLDNDLIVLDGQTIKR"
     gene            28135..28737
                     /locus_tag="PK105_02255"
     CDS             28135..28737
                     /locus_tag="PK105_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60560.1"
                     /translation="MKEEPSISPDQQLELIERMLEESRKTVLSMGFPSMIWGGFVSIG
                     TALSYAFASWHWYVAIGLLWALLMVSAFLLVVRHDRRKQQQQLKTTSLHKISNEMWIF
                     IFGVMVLYGIVGFLRPQNISLPYALCVVSLLVGTAYWISSSMTRYFLLRIMSALWIVC
                     SIVVLLVPALWSPAVVGICAFLGECMPGIYFYAKERKESV"
     gene            28709..29068
                     /locus_tag="PK105_02260"
     CDS             28709..29068
                     /locus_tag="PK105_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOU60561.1"
                     /translation="MQKNEKNPYNYQSFDEMLSSRIRLAALALLAGCDEAEFTYIRDV
                     IQTTDGNLASHLRKLEGSGYVRYQKRFIDRKPATYYRITPQGRSALLAYSRRLTELLK
                     NSNYQGGSSNETETDEK"
BASE COUNT         7898 a         6987 c         6215 g         8283 t
ORIGIN      
        1 tttccccctt gcggctcctt ccattggcca tttataaaat tctcgaattt ttgtggttcg
       61 gccatattta attctccttt caaataacac gctctctata gaatgcggta tctatatatt
      121 ctcaattacg catagccaag caaccgaggc aaccacattg aaagcggctc aaaatatgta
      181 accaacaaga gcgcaattat cgacgcaatc aagaaaggtg caagtgcttt gctaacagcc
      241 tcgataggcc tctttgcaat attgcacgct ataaaaagac ataggccaac agggggcgta
      301 ataaggccaa tcatcagatt taggactaag atcgtcccca acattatagg gcttattcca
      361 aaaggcttgg taatctgaag caacaaaggt gatagaatga tgatcgctgc tgttccttcc
      421 ataaagcaac ccacaatcaa aagaaatata tttataagta agagaagaag atacttattt
      481 gttattgtat ttccgatgaa taaggctatg ctttgaggaa tctgctcgac cacgagagcc
      541 cagttaaaca cattagagca cccgatgaga aggaatacaa cgcctgtagt ctttgccgtc
      601 tctatcagag ctcctctaat atccttccat cctatagtct tgaatacgag aaggcttatc
      661 aatagagcat aaagcacggc tacggccgct gcttccgtag gggtaaaaat accaccaatt
      721 atgcctccca atataatgag cggcataaag agcggtatga tccctcggcc aattgctctc
      781 gccacttctt tggggctcgg ccaacgctca gaacgaggat aatttctttt cttagatata
      841 attgcgttgg caatcatctg gcttatgcca agaagtatgc ccggtatgag accggacata
      901 aaaagagcgg caacagaggt tgcactcact agagaatagg taacgaatgg gatgcttggc
      961 gggatgatag gtccgatgac tgaagaagcg caggtgatcg cggccgcgta gtccgaatca
     1021 tagcctgccg ccaccatggc tggtatgatt attggcccct cagaagaagt atccgccacg
     1081 gcagaaccag ttatccccgc aaaaaacatc gaggtaagaa cattgacatg cccaagtccg
     1141 cctctaagcc ttcccacaaa gacatcggct agatctatca atcgcttgga aatccccgcg
     1201 cgattcatga gctcgccagc taaaacaaag aaaggaatag ccataaaggg aaacgaatct
     1261 atgccggtaa acattctctg cgcaacgagc ccaatgggtt tcccctctac cagaagataa
     1321 atcagacttg aagaacccag gctaaaggaa acaggaacac ccataaatag aaatactgca
     1381 aaagcaataa cggcaaatat catttattaa tctcctgtct ccgttgattt tcatcatcaa
     1441 aaaggcgaat tatgcccaat atattttcga ttgccatgaa taccatacca gcggtaaccg
     1501 aaaggtatgg caaagccatt ggaatttcca aggctgccga acgctgaata gaaagagtct
     1561 taagataatc aatagactcg ataaaaacca caatcaagaa tatttccacg accaattttg
     1621 caatcacttt ggctatgaat tgtatggaat ttggaagagc tcttaccacg aaatcaatgc
     1681 ctatatgagc atcatccttc atggctaggc ccgctccgag catccccgcc cagatcatca
     1741 aatatctgct caattcctct gcccagaaaa ttgaattttg aaggacatac ctaaaaaata
     1801 cagcggtaat aatgtcgaca accattatga ttagcaaaac tacaactatc catcgaactg
     1861 cagactccaa ttttccgaca gtcttcataa acagactttg ttccttcatc ctttgcccct
     1921 taatctccca taagaattac agcaaaatcc tagaaaggat gcggtgctca aaaacactct
     1981 taaaatgcca acaaaagggc gcaggaacgc aatttagatc gcccctgcgc atctaatcta
     2041 cagactgcta tttgagtcct tcttttgcct tttgagccac tatggcatca tctactgctt
     2101 tcaatagttc attgataagt gccggatcga ccttagtttt aagccaatct ataaccgctg
     2161 gctgcatgac cttcttgaat tgttgcatct cggcatcggt tggagagtaa acctgcatcc
     2221 ccgcatcgat aagagtctgg agaccttggg cattctggag tgtgataaag ccacgaccta
     2281 ctgcagcaac tgtctgtgcg gtgtccacaa ctatgtattg cagatctggc ggcaagctct
     2341 gaaagaattt ctcgttgatg ataaaccaat caactccata gctgtggcca tcgagcgtga
     2401 tgtatttctg tacttcatat agctttgcca tgattatcga agcgattggg ttttcttgtc
     2461 catcgacaac atgggtttgg agagctgtat aggtctcggt ccatgctatt ggcgtcggat
     2521 ttgcgccagc agccctaagc atgttgacat ataccggagt ctccataacg cgtatcttca
     2581 tcccttttag atcacttgga cttttgatag gacgaacact atttgtgaaa tgcctaaagc
     2641 cgacctctcc aaaagccaga cagcgcatac cagtctgttt aaggaacata tctcgaagct
     2701 tgtcaccgaa ggccccatcc atcacatccc aggccacctt acttgttgga aaaagatagg
     2761 gaatatcggt gattgcagca ggagggaaaa aatctgctac aggcccggaa gcaaggccag
     2821 cttcgatagt accagccttg attccttcga tgtattcacg ctcagctccg agagctcctc
     2881 ctccaaagat ctttacattg atacgtccgc cgctcctgga gttgaccatg cttgcaaatg
     2941 caatagcctg cgcttgcttt cttgagaccg caggatcggt aggatctgca tgtgcaagct
     3001 ttagctcata taccttgccc tgtccaaaag ctcctgatag cacaatgcat accaggagaa
     3061 ccatcaatcc caattttttc ataggaatac ctccttcctt atatgatgaa gccttgcttc
     3121 gtttttccat ttatctagct aggccaagca attgtatata tgtgctttcc tccatttagc
     3181 ggacctttga gaacgagttc gatttccttc tcgtctcttt gagtcataag cttgatttct
     3241 ggcagcttgc cgacaggaaa acgttccatg atgtcattgt gtagtctgac catataatcc
     3301 aggacatttt cgatcgcttt ctttgctttg ttcggatcag cgagttttgc ttttcctaga
     3361 acaccttcgg ggaacgtcac acattcgatg cctgtcccgc caattatgca atgcccagga
     3421 atcgggttgc cataaatatc tccgcctcta tcgacatgtc ctggcggaag aaaacctttc
     3481 aatgatgtat tctcggcatg ttccggttgg catagttccg gaaacaatgc catcgaaatc
     3541 gaagtctccg cttcatcggc atgctggaat ggcgtttcga aaggtccacc atgttcttta
     3601 tctttgagat ctgttcccat tattctcggc agatcaagga agatgacgat tgccggaacc
     3661 tgatatttct ttccccattc ttgaattgcc gatggaagag catattcttg accatgcata
     3721 ctgaggaata tctgcttacg gaagccagcg ttccagaatc cagatatcat tgcgcgaata
     3781 tgagccgtaa atacatcgtc agatagaggt acggttcctg gttgcccaat atgatgaaat
     3841 ggatgagaac cataccacat cggcatcgct accgtacagc caacttgctt cgctaccaat
     3901 tccgctatcc tggtcacgat aaaagtgtct tcaccgtatg gccccgcatt tccatgattt
     3961 tcggtcgacc ctataggaat tagtatcaag tcgtttgtct tcaatctctc ttctacatcg
     4021 tatttcgtca tagtctgaaa atagattttt gagggcttgt ccatctggcc ttcttgaggg
     4081 aatttccatc ctgacatctc ttgttctcct ttcatttctt aattattcgg aatcctatga
     4141 ctctttagct gaggggcata aacccagaca aacttgagtt tagttgtgcc tgtattcaca
     4201 attttgtgcg gggctccggg tgggttgtag acacatattc ccggttttag gtctatctcc
     4261 tgatcttggg ttatgaattt gccttcgcct tccaggaaaa acataacctc ttcctcagtc
     4321 tcatgtgtat gttcggggac catcgagcca ggctcagttt cattgacacc aagcgcaagg
     4381 ttttgagccc ctacggtctt ctctgaaatg agtatccaag atgagcgcgg aggggttttt
     4441 ttttctggct ccactttatt gatatcaatt accttaagtt cactcatcat ttcctccttc
     4501 tgctttattc ttctaagcct atttgttacg caataaagat gcccccattg acatccagtg
     4561 acacacctgt aatccaattc gaacgcctgg atgccaggaa taccaccgca tcgccaatat
     4621 cttctggcaa tccatcttta gggactgccc tccccgcatt ccaggtgtcg aatacctctc
     4681 ttggatattg ctttgtctga tctgttaata ttggaccagg acagatagca ttgacataga
     4741 tattcgacgg tccgagttct tttgcaagca gctttgtaag cccaatcact cctcctttag
     4801 aagcagcata gttgacaccg acgcctggtc gacctgttct gcctcccagc gaagcaatat
     4861 tgacgatttt tccgaattct tgctttttca tgacaggagc cacagccttg cagcatataa
     4921 atgttcccgt gaggttcact ttgataaccg catcccagat ttccaatgtg gtctcttcaa
     4981 gaggcttatg acgggaaata ccagcattgt tgaccaatac atcgatatgg ccgaatttat
     5041 caactgtagt agcggccgca ctttgaaccc cggattcatc ggtgatatcc acttttaggg
     5101 gaagtactct ctgtccgcta tgatccagtt caaaagctaa tctctttaca gattcaaagt
     5161 cgatgtccca caaggccaga cttgcgcctt ccgcaaacat cttctttgct atgcccatgc
     5221 caagaccatt acctgagcct gtaatgattg ctatagtatt ttcaagttcc attttctcta
     5281 gcctctcagt ctatctgcat atatcttact tttattcggg aaacaaaatg acttttactt
     5341 ctttggtatt gcccgaagcg agctcctcga acacctttgg cccttcagaa agcgggtgcc
     5401 gcgaagtgat catctgagct acgttgagtt tggcatctgc aatcatctgc aagccttctt
     5461 tcatttcggt tgtatacata tagctggcct gcagttttag ctccttgctt accgcatgct
     5521 tatacgcaat ttccagggtg ggcgatgcca tgccaaccat aaggatgctc cctccatttc
     5581 ttacgatttc gatggcagta tttactgtcg aaggaacccc tgcggcatca aagacaacat
     5641 cgatccccac attgtctgtc atctgccgaa tgagggctgg tacatcttgg cccgagagta
     5701 ttgtatcggt agcaccacat tttcgggcaa gttcaagccg attttgaaga agatcgattg
     5761 cgactatttt tgcgcttcca agattcttca tgcacataat agccattaga ccaatgggtc
     5821 ccacgcccaa taccgctact gccctgctcg gtacttcatg ggccacgtgc ttggtcgctc
     5881 gaagcgatat ggacaaaggc tcaacaagag cggcttcgtc gaatgagaca ttatctggca
     5941 agagcattat tttctctgct ggagctacga caaattcgga catcgcaccg ttgtaacttc
     6001 cggctttcat tgttgtaccg atgatataac ggtgatcaca cacatttgtt ctgccttcta
     6061 agcaatccga gcactttccg caatagatgt tcgtgataac accgacacga ttcccaacct
     6121 tgagattatc tactccttct cccagttgaa ctatctcacc ggagaactca tgccccatta
     6181 tcagacccgg tgacctcata gaggaaacag ccttataggc cgcaagatct gagccacaaa
     6241 tagagacagc tcttacatgt accaggactt caccttcttt tggccttggt gttggaacat
     6301 cttgatatcg aatatcatga ggtccataat ataccaatgc cttcatagaa tgcctcctta
     6361 ttggaatcaa gaaatgctgt gatatggctc gatattatta gccttaaata attctaagcc
     6421 ttccctgatc tccttagcaa agtcctgaaa tacgcatgcc tcgatggata taatttcttt
     6481 ataagcaatt cttttgagtg tgtttagaaa aggagcataa tcaaaaccat cattcatccg
     6541 cggcacaacc cttgtgggaa atggcgacag aggattgtct agatgcacat gtttgatata
     6601 gagctcatag tcttcgatat ctgtgagagg ccaaccatct tctaacatcc accgatagtc
     6661 taaaagagtc ttgagattgt gcttttcata tttctttatg aattcaattg cctcagggag
     6721 cgaattcaca atattcgata gactcttagc cagcggctct ataagcaccg tgatattccg
     6781 ctttgccgct atttcgagaa gacttataaa aaagtcatcg aatttactgg ccgctatcga
     6841 aatatctcct tctgatggaa ggcttcgagc tttgccattt ccaaagacaa tatagcgcgc
     6901 tcccatttca gcaattcgat ccgcgctttt ttcaagaaaa tctcgaaaat gatccaaata
     6961 aaacgaaggt gatgatatgc agcaatcgag aggaatagga ttatcgaata cttcagcagg
     7021 aattccacaa tctctcagcg ctttcttagc ttttttgaat tcgccttcgc tcatggccat
     7081 gatctctcta atgtgcagct ctgcgcaatc aaaaccagcc gccgcaatcg tgtccagatc
     7141 tttgatatat ccaaaacagc caagccgcat cttcatcttc gattccttat caaatgcccc
     7201 tttccatctt tcatcgacat tggtccatcg gtctcagcag aaaattagct cctgcttttc
     7261 gtaatagctc atttaggtca tttatcactt ctaccggcat atcgataccg ttttctcttg
     7321 attcttgttc ttttctgagc tcgatttctc ctggtaagag tatttcttcg aaaccctcac
     7381 gaagttcaca agatttcaag gctctggcat actctccgac taaattcctg aatttatcca
     7441 cggccataaa gcgcgaaata tcgatagcta tgaagcaagc tccgactcct gttttgcctt
     7501 ccagttcatg gaagctcttc agatttcgtc cataagatga gcccgagagt atgccagcaa
     7561 aaatatccac aattattgcc aatccagaac cttttgggcc acccacaggc ataagacatc
     7621 ctttaagcgc tttttgtgga tcactggttg gattaccttc gctatccaga gcccaatcta
     7681 gaggaatatt ctcattttta agagcagcca ttctgatctt gccccttgcc acaactgacg
     7741 acgacatatc cagcatcacg ggcatttcgg aagaagtagg taccgcaaaa gcaattggat
     7801 tggacccaag aaaaggctca gcccctccat acggcgccat ggaaggattt ccatttgtca
     7861 tcatgatggc cgccatattt tgcatggcag ccttgtgcac ataataacca agtgagccta
     7921 cattattggt atctctcaag agcgccatac caagaccata cagatgtgct ttttctatag
     7981 cggcattcat cattttgtag cctgcaacct ggccaagccc atctcctcca tccaatacta
     8041 gagtcgccat atcttcggaa acaacgacaa ctcttgtggg aagactgagc atttttgctt
     8101 cgcatctttt tgctatcatt cgaaggagat ttgtaccgtg tgtacttatt ccccgcagat
     8161 cattgataac cataatctca gaggctattt gagcattctc tttactttct ccgacaagcc
     8221 ttagaatttc gcacgccgcg ttctgtagat cccgatagaa tatcttcata tcctcactct
     8281 tcctctttca tgccgagtgc gatacagaca tcttcgagct tatttagttc catgctcgaa
     8341 ataccgcgct gaagctgctc tgatttcttc ttttcatttg cgactctatc aagcgacttc
     8401 tgaaggacat cagctagatc ctgacgtcga accaccacca ttccatcatc atcgcccata
     8461 attagatcgc caggataaat tgttgcacca ccaaaattga gaggataatt gattgttccc
     8521 aggccatttt tggccgttcc tctaatgcat gctccgcgac agaagacggg aaagtccatc
     8581 tcaataagct cttctgtatc gcggacacaa ccatctatgg cgaggccccc tagttttgcc
     8641 gttatggctg ctgtccccat cagcgaacca agataaccat aaaaataccc atctcccatg
     8701 cttgcaacca acacatcacc agcttttgct gtctgtaacg ccttatgcag cataagatta
     8761 tctccaggaa cacactgtac cgtaaaagca gggccacaga ttctcattcc tttagcaagc
     8821 ggttttatag cacaatctat gaatcccttc ttgccagaag cttcgtagat tgttgcaact
     8881 gctaattttc taaatgattc tatgatattt tggtctggtc tttcggcaat ctttagaaca
     8941 tgaatcatgt tgtcctcctt cctatagcag acttttaagg cgttcctgac tatttcttaa
     9001 gtgttctctc attgcctttt cagcagcttc ggaattgcct gcaataatcg ttttcaagat
     9061 catcttatgc tcagcattca ttctctctgt tttttccatg tcgaattgat agatgaaaaa
     9121 gaggcgtata cgtattatgt gattctggat attcttatag aaatctaaaa gtcgagaatt
     9181 tccgacagtc tcaaaaatgg cagtatgaat ttcgaatcca gagactacgg agcctttgat
     9241 attcttcttt tccgcaagtt cgaatgattc atgattcttg agtatctcct caaatatcgt
     9301 cttgtctcct cgttcggcaa caatgcgagc cgcagcacat tctaggattt ctcgaatttc
     9361 aaagatttca tgtatataat ccagtcctat gctagaaacc agacatccta tgcttggtat
     9421 tattttgaca aatccatctt tttccagttc cttgaaggcc tcgcgcaccg gagtcttgct
     9481 tattccaagc tcgctgcaga tcaattgctc agtcagggta tccagtggct tatattcatt
     9541 attgattatg cgttgcttga taatctcata agccatgttt tttttgtttg ttgcatcgat
     9601 ttgatctttc tgtatcattt gcacgaactt tccccttttt taaaggtatg ccagcgatat
     9661 atcggtaaga tacttttagc ataagtccgg tttttcgttc tgtcaatatt aaatttgttg
     9721 atatttcgag aatctgataa aatgagcttt tctagagaaa tctcagtata agcggtacat
     9781 tgaactagcg gtttatttta agagattatc ggtggctcta ctcccagttc ctgcgcaatc
     9841 tgcctgagct gcaaagcaac cttttcgctg atcggaacac caaaatgggc gctttctgcc
     9901 tctgcttcat gctctttaag tcccgcatag tagactcgct cgcagccttc ggctggctcg
     9961 gccatttcga gatcggtcag taagcgatcc atatcggatt tgatagcttc cgcatcgcgg
    10021 aaaagatcga gtcgcagcgc accaaagaag tgacaaaccc tcgctgacgt agcctgacta
    10081 tcgatgacag cttgaccaaa aacaccgccg ctcaatatcg cagtcagaat atccactacc
    10141 tctgctagtc cataaccctt gtacccgctc aatagctcgc cttctcctcc aagcggaaga
    10201 agccctcctc cccgctgata aagcatgtct tccagaagac ggtgtgcatc cgaagtgcct
    10261 tttcctctcg tgtccacagc ccaaccgctg ggcaaaggct tattctctct atcgtagact
    10321 tcgatttttc ctctggtaac gacggtcgtt gccatatcga gtgtgaatat tcgcccatca
    10381 tgagcaggta ttgaaaatgc aattggattc gtgccgaaca tggccttgcg accaaatgta
    10441 ggtacaccga gtgctgccgt attagtcatg ccaatgccta tcatatcctc tcgggcggcc
    10501 atctcgctgt agaagcctgc aattccaaag tgattggagt tgcgcaccga agaaaaggca
    10561 gccccggcca cccttgcttt ttcgatgatc ttagccatcg tcctcttgct tagagaaaga
    10621 cccatagccc catttgcatc gagaactagc gaaatgggcg tctcctggag agtcgtcggc
    10681 agaatcccgc ctttcataat gcccttttgt aggccattct tatatctcca taagcgccct
    10741 acaccatggc tctctatacc acgcgcatcg gcggcaacca gaatctcggc tgaatcttga
    10801 gcctcatcct gcggaagtcc aagcttaata aatacatcag tacaaaactg ttcgagcctg
    10861 actcgatcta tccttatttt ctggctcatc aagcgcctcc tttatctagc gccttttctc
    10921 ttctttatca aatgagatgg aactcaatct cagtatactg caatgtgatc ctaatggagg
    10981 aatgctcaac tgacaatcca ggcagtttgt ggactcgaca catacaaact ggtcccatgc
    11041 gctaaagatc tcggaattta ccgcgcctac atctttacct ggattccata gcactattga
    11101 atccatattt tcttgttcaa tgcatattct tcgattatca acaagatcta caagttccaa
    11161 gcgctgcgga gcatgcatga aagcatgaac aatctcgcct tcaaagcagg ccaggccatt
    11221 ctgtctttct cggatatctc cttttgtcct gtctatgtat tctatcccat ccaatcctct
    11281 gacctgacag ccatgaggat tatttacctt aaaatatgta tgaaatccat cttcgcagat
    11341 gattggtaga ttttcgaggt tttgtattat aagcgctatc tcgagctttt ctccaacagt
    11401 aattatgatc ttaagtctaa aatgataagg ccatgaatcc ggaagcggtt cttcgctttc
    11461 tgtactaagc tcaatcttcg tccgtccatc ggtcagatcc tctgctgatt cgatattcca
    11521 gaactctgtt ctaacaaaac catgtagagg aagataatcc attgttctgt gcttactgaa
    11581 ccaaggaaaa cagattggaa tccctccacg gataggtgtt cctttgctaa agcggctata
    11641 agggctcagc cataataatt ctgtctggtc ttgaggcttg aaactcacaa catgagctcc
    11701 atacaaataa agagaacatt ccgcatatct attaagaata tggagaagag gaaaccctcc
    11761 tttcccttga atgacctcta cgctttttgg tagagcacag ttttgaaaat gccggacatc
    11821 tctcattttt tatcttgaca acctcttgaa tgatgatatc atctatctta gatattagca
    11881 aggttcaagg aggcttgaag tgaaaaagaa gtcgatggtc ttagttgtct ttttcctaat
    11941 cgctgctatt tttctggtct cagcccaaac agagaaaaaa tacgttctta aatttgggca
    12001 cgttctaacg acacaggatc cttaccatga ggcattcttg aaaatggctg aagcagtatc
    12061 agcaaggaca aatggcaatc tgaaggtcga ggtatatcca agcgcacagc tgggtgtgga
    12121 ggaggatatt ctggagcaga tcagacaagg tgcgaatata ggatggaaca ccgacgcagc
    12181 aagactcggc atgtatgtgc ctgcaatcgc ggttatcaac gctccctact ttgtcgatag
    12241 tctcgaagaa gtcaagagac tcaatacaat cccttcagta tctgcatgga aggctgaact
    12301 cgagcgtact caaggaatta agattctctc gttcatgtgg gtccagggtt ttagacagat
    12361 gataaccaat aagcctattc gcaaaccggc agatctttct ggtttacgga taaggactcc
    12421 tcccgcgccg atctggcagg aatccgttcg agctattgga gccgttccgg ttgcaatggc
    12481 ttatggggag atgtattcag cgctccagac aagagcaatc gatggccttg aattatcctt
    12541 tactgcgaca gcaagcggca aattccagga agtagccaag tatgcatgcg aaaccaagca
    12601 cattctcctc atcaatttcg aggtggtatc cagcaagtgg ttcaactccc tacctgtaga
    12661 atatcagaaa atcctccaag aggagtgcga taaggcgggg ttggaagtct ctcgacgcta
    12721 tctcgaagaa atcgatccga agaatatcca agtgcttaag gcaggtggca tgaccattat
    12781 tcctgcaagc gaaattgaca tggctgcctt caaagccgca ggcgagaaag cttatcaaaa
    12841 actcaacttg gtcgcggtgc gagatcaggt ctataaggaa ctcggaaaaa caaagtaata
    12901 tctttcgggc ggcgtacctc ctcggtagcg ccgcctttac ttctctcttc catatccata
    12961 agaggagccg tttgcatgag aaaaatctat cattttttcg gaaaggcaga ggtcctggtt
    13021 tccgccgtct gcttcactgt ttctctattg ctcatattct ctggcgctat cgccaggacc
    13081 ctcagacacc ctctaaactg gtcgcaggat ctatcgctct ttctattcgc atggggcgtc
    13141 tttcttagtg cagatgccgc aatgagggct gataagcttg taaacattaa tttgctcgta
    13201 gctcgcttat ccgaaaatat gcaaaagatt ctcgccacag tcgtctatgt cataatcttc
    13261 atttttttga tttttttatc ttacaacggc ataaagctga gtattttttc tcatcgaaga
    13321 gtattccagg gtattccggg ctttagttac tcgtgggtca tggtcagcat tcctgtagga
    13381 tctgtgttgc agatgatcac catcattctc aagataaggg atctctataa gagggaagaa
    13441 cactgatatg ttaatcgttg gaatcttgct attagccttt cttgctattg ggatgcccat
    13501 cgcttatgca attggtatct ctggtttcgc attttttctt caacatccgg agctgcccat
    13561 gaccattatt gcgcagttac cgatatctca gacacaaaac tttacattgc tggcagtgcc
    13621 cctgtttatc cttgctggca accttatgaa tgcttctggt ataacgaata ggctaataaa
    13681 gctctctacg gtcttgacag gtcacatgcg gggtggcctt gctcaggtca atgtaatgct
    13741 ttcgactctc atgggaggag tgtcaggttc agccatagcg gatgcggcaa tggaagcgcg
    13801 aatcctctgc cccgctatga tagaaagagg ttattcgcct ggcttcacgg caaactgtac
    13861 tgcttggact gggcttataa ccgcaacaat tccacctgga gtaggtatta ttctatatgg
    13921 tgtaacagga gaagtatcta ttggaagact ttttgctgca gggatatttg tgggaatctt
    13981 tctcacggcg gtatatatgt tcaccgtatg gatcatttct gggcgcaaag gttacaaggc
    14041 tgaacgcgct cgggcgtcat ttcaagaatg catgcaagcc attgtagaga atatctgggc
    14101 attgctattc cctattcttc tccttgtagg tttgagaatc ggggtattta ccccctcaga
    14161 agtgggttca tttgcctgcg tatatgcagt cttcgtaggc atctttgcct ataaagaact
    14221 cagcctaaag aagctaatac aaacattggg ggaaagtgta atggatgttg ggggcatcat
    14281 gttcatcatc gcgctctctg gcatctttgg ttacggaatt ccttttgata gaatccccga
    14341 ccttatttcc aatgcaatac tcggtttttc gaccaatcgc tatatcgtat tgttcataat
    14401 actcttcata ctcattattc ttggaatgtt catcgatggg tcagtagtta ttttgctctt
    14461 tacaccaatc ttccttcctc tggcggctca gctagggtgg gatccagttc attttggaat
    14521 tctcttctgt accatcatta ccatggggaa tatgactcca ccagttggtt tggccatgag
    14581 tgcagtatgt actgtgctaa atgtgcctat atcggaatat ataaaagaaa tgtggccatg
    14641 gttttgggca accgtgcttg ctgtctcggt tttggtgttc ttccctggcc tcgtacttgc
    14701 tttgccaaga ctgcttttct gagtgagaga ataatatgga attacaagga aaaactgcta
    14761 tagttacagg ttcagcaaga ggaattggat ttgagatcgc catggttctt gcccgagaag
    14821 gagcctttgt agtgttagtc gatcagaccg gcacacaaga tgcggcaata tctctgcaag
    14881 aaaagcacta tggcgcaatg gctatctcgc tcgacataac ggatttagac gcagtcaaca
    14941 atgctgtaaa gacaatagta agcgcaaaag gtcggatcga tattcttgtc aataatgccg
    15001 gtataatagc gcgaggtagt atcatcgatc tgagccgaga acaatggctg cgagttatgg
    15061 atgtgaatgt caatggcaac ttctattttt gcaaggcaat ccttccatat atgattcgcc
    15121 aacactcagg gaacattgtc aatataactt caattgcggg aaaaatgggc gatataacag
    15181 ctgcccctgt ctatggagct tcgaagggag caatcaatgc ctttacaaaa tcgcttgccc
    15241 gccagcttgc ggaatatggc atcagggtca atgccgtggc tccccatgct atagagacca
    15301 gcatgagctc agaatggaca atagaaaagc gggaaaaagt catagatgca attcccttaa
    15361 agagaatggg caaacccgaa gaggtagcgg aggctgttct cttccttgct tcagaaagat
    15421 cttcttttat caccggtgaa atactgaatg taaatggtgg aatgctgatg gattaagatt
    15481 tccaaatcta tactgctcgc ttgctacccc cttccttttc cgaggaaaag aatcggccgc
    15541 ttagaatatt ctgcctgttt gtgcgcaagt gtctttcgat gatcttaagg gcttccgaag
    15601 agtgagcagc caaggcattc accagatcta tatggtccct aaaagtctga gagacatttg
    15661 gttctatacc tatatcgtat acataactca tattgagatg tatattcagt tccttataga
    15721 acttattcac aaactgatta tcaacaagat tgcataactc tttatgaaat tgccaatcgt
    15781 gctttatgta ttttctaagg tcgatcggat cttcggatat agccattttc atctcttcta
    15841 tgatttttct cagcacatca agacgagaat tttcgataga ggcatatatt gactttacac
    15901 tatagatctc gagcatctct cgagccgcaa ttgcatccag tatggtctct cttgtgggtg
    15961 ttctaagaag gcacatagag cgaggtttta tctccacaat gccatcagtt tccagccttt
    16021 tgagcgcttc cctaacgggc atcatactga tattcaactc ttcggctaag cgtctagtat
    16081 tgatagtttc gccatacttc atctttccat tgctgatcga attgcatagg taaagataga
    16141 cttgctcagc caggctccgc ttttcgatga tgatattgtc ggattcttcc attttcgatt
    16201 cctttctcgg tgatatctct tggacaatct agaatacacc cataatcgct tgaaatcaat
    16261 aatatcagaa tctgatatct attatctttt taattatgcc ttatcgcgcc ggcacttgca
    16321 tcgacttgga catgccgcta ttgtattagc ccactgatat caaaattatc atttgtgttg
    16381 ctgactttat tcatttgatt gatatatccc acatgctagg ctgcattatc gtcgacccat
    16441 cgtagaatat cttgattaga tttcaaaaag agaatagaaa tatttcataa tctatgaaac
    16501 tggccttctt gcatcagtcg actgagttgc ggcgcgacta tattctaaat ggcccacttt
    16561 cttctacttt gatgcttctt gcagctccta cgctcatgct tggtgtcgtt caggcactca
    16621 tgcctctcat ggatggactg tttatcaaca atatcgcggg tactcttgta gcgagttctg
    16681 tcacgttcag cgagccagtt attaatatga cgacggcttt ggcacaggga ttgagtgttg
    16741 ccgcaatggc catcattggg cagctcaatg gacgtggtga cctcaaggaa tcaaagcgta
    16801 cttcgacgca gattctggtt atgggatcga tgcttggttg tatttctgca ccttttctag
    16861 tcctggcggc taagattatt tcgtcgcgcg tcaatccagg tatttctcac aacgtttttc
    16921 tatatctttc gctctatgct cttgtacttc cattttcctt tatggagtct atttacaacg
    16981 gtattaagaa cgcaaatggc atgccagaag cgccattcat aagaatggta ctcatgctca
    17041 tactcaagat tgccttcaac ttcctctata tctatgaatt tcgtatgggt attgtgggtt
    17101 gcgttctctc ttcgctttct gcgaatatca ttatcactct atggatgttc tgggagctat
    17161 ttctaaaaaa ggggcccgat agactggagc tcaaaggatt caaattcgat ccggtcgccg
    17221 caaaacagct ttttcgcata ggcgcaccgg ccatggcaaa tacttttatt ctcaatctcg
    17281 gttttttctt aatcaacacc gaagtggaaa aatatggacc tgtggttctc aatggacaag
    17341 gcatagccaa caacataacg caagttgcct tcaatgttcc cagcgcattt ggttcggcag
    17401 ttacgaccat ggtcagcatg aatatcggcg cggagaatcc aagaaaggcc agggaatcta
    17461 tggtccatgg cactactctc agcgccttaa cggccgccat acttatcgcc ataatagtcc
    17521 ctctctcatc gaggctgact gtactattta caagaagaca agatgtcctc gaaattgcga
    17581 acagggctct gcatatctac acttattccg taataggctt tggaataacc actacgatcc
    17641 agggggcatt catagggctt ggccgaacca gagtcccgct ggttttggga attctgcgaa
    17701 tctggttatt gcgctatgct ttcattctcg tgacagaaaa gtatctccag tattatgcgg
    17761 tcttctgggg aaacttgttc tcgaactatg caagcgcgat tatcgctatt atcttaattt
    17821 ccagaactaa atgggaatcg gcaatcggaa aatcaatgca attggaggtc gccagtgcaa
    17881 aatagctggt gcgaactgcc agcgacaatg tacagtgttt cggctgtccg gcatgaatcc
    17941 caaaagagac ctagatcagc agatccctat cctccgcata ctccgatcca cgcacttttt
    18001 tgaataagcg aacaagcgcg gcaacattca ttttttcctt ttctggaccg cttagatcgg
    18061 ctataatctc accttcatgc atcattatca ctcggctagc ttgctccaac gcgcgcttca
    18121 tatcgtgagt gattatgaga gcagttaagt catattcatt gataaatcgg cgcgtaaggg
    18181 catccacaat ctccgcattc gccgggtcga gtgcagccgt atgctcatcc agcaacaaga
    18241 tcgtcggatg agagataacg gccattagaa gcgttagcgc ttgtcgctga ccgcccgaca
    18301 gtcgcgaaac attttcccga aggcggcttt cgagacccat gcctagctct gcgagctttc
    18361 tctccatttc tcgggcaaat cgcggaggtg tcgcaatgcg aaatcggcga ggccctttgc
    18421 gagctgcgag agctagattg tcgattaccg tcatttcgcc agcagtccct gccataggat
    18481 cttggcgcac tcgacccaaa tacctggcgc gctcccattc tgcccaggag gttacatcct
    18541 cgccatttag atgaatatgt ccagcatgca atggaatact ccccgcgata gaattgagga
    18601 gcgtgctctt gccagcccca ttcgaaccga tgatcgacac tgtctgacct tgctttacct
    18661 caagtgaaag cttatttaga acagtgtgtt cttgcccacc tcccaaatca aagcttacga
    18721 cgacatcttc tatgcgtacc ataaaatcat ccttcttccc aacttttgtt catcagtgct
    18781 tccttttgat tcgagaaaga gccacggata gtattataag aagcgcggtc aaaagcctta
    18841 gatcattggg ggtaataccg gcaacatagc cccatgagcg agccgcgtac atcaatgctc
    18901 taaacaagat cgatccaatg agaaccctga gaagctgaac gcttatgaat tgcgaacgga
    18961 taatgagctc gccaagcata actgtggcaa ggcccgaagc caccactccc tgtccgagat
    19021 tgacgtcggc aaaaccctga taagcggctg ccattgcgcc ggaaagacct ggaagagcat
    19081 tggcgagcat tattccccag gttctcaatc tatttggctg aatgccagcc tgaatgacgg
    19141 cattctcatt gtctccaagc gcacccatcg caatgccaat ctcggtatga aagaaaagat
    19201 ctagaagccc aaagagcaca aggcatacca gcacgcatga tgcaaagagc gaccactccg
    19261 agggaagcgc acctccgaga aatgaacgcg caagcttgag catggggttg ttagttatga
    19321 gtggatagtt tggctttcca ccgaggatac gcagatttat cgaataaaat cccgtcatag
    19381 taactatgcc tgcaagcagg ggcgggattc ttaacttgtg ataaacagcc gcagtggcaa
    19441 aacctgctat ccctcccaca acaaaacccg ccagcatacc agccggtacc gccaacccat
    19501 gcagaacagc atttgaggat agattgaaag ccgaagctac tactgcgcct gcagcagctc
    19561 cagcaggaaa agatccctcc acggtcaagt caggaaaatc cagaattctg aagcttacaa
    19621 aaactccgaa cgcaagaatg ccatagatta atccttcgac gagtattcct tctaccatcg
    19681 attacttccg cacaagttga tcatctgaga taactacttt cgccctgtcg attatagact
    19741 gatcgactga tatgccgatt ttccttgcag tatcgagatt gagtacgaaa gtcatgtctt
    19801 cattctcttt ggacagaaat gcagggatat ccgatgtttt ttccccattc aatatgcgca
    19861 ccacaatttt gccagtagcc aaacccatgc gatagtagtc gtatccgaga gccgccagca
    19921 ccggtattgt ctccgcagaa gagggatcag cagtcacgat tggaatcttt ttatcgattc
    19981 ccacttccgc aattccgcta agcgccgaga agaccgtgtt gtcattgccc aggtatagtc
    20041 catcgattcg ccctgcaata gagagaagag cctgcctagc ctcggaagaa ttcgtaatag
    20101 tcgactctac atattctaaa ccatgctctt ggcaatattc tctgacgatc tcagctagag
    20161 caactgagtt tgcttctcct gaagcataga tatttccgat gcgctttatg cttggtttca
    20221 atgaacatag aagatccagc tgttcccgaa caggggtcat atcggatgtg ccggtaacgt
    20281 tgggtccgcc cttatccatg ctttctacta gatcggcggc aacaggatcc gttacagcag
    20341 aataaatgac cgggcgatcc ttgatctgat ttgcgagtgc ttgagccatt ggcgtcgcaa
    20401 ttcccacggc gagtgcaacc ttctcctgtt taaagtgctg tgcgatctgg gcagccgtat
    20461 tcatgtctgc attcgcattc tgcagatcga ccttataatc agcctttgat gcagagagct
    20521 catccaaaat gcctttctct aatgcatcga gagccggatg cgacacgaat ttagccacac
    20581 cgacaacttt tgcgcccttt ggcgcacaag ataacaatag caagatcccc gcacatccaa
    20641 tcgcatagag caattttgca aaacgattac tcatacaacc tctcccttat acggcaaaat
    20701 gatctgtcca tgccatagcc attggcattt gcatgtcagc aaatctatgt tactatgcat
    20761 agtaacattt tcggcattgt atcttcagta tggcacttcg tcaatatata aaatgaaaaa
    20821 aattcgataa tttctgagaa attatcctga ggctgctttt agtcaagcct ttgccgaaaa
    20881 gtatttcgaa tattaaaacg aaatatgcaa agattaaaat attatgcctt ttagaaataa
    20941 aatgctttac ttttgataga aacaccttga cacggagtat tgggatatat attcttcgca
    21001 gaaattggct tcattccatt atttatgcgc atgtttcgta tatgcggaag gaggaattca
    21061 aaatgaagca aagaaaaatg ctctttgcag ccattgtgag ccttgtgctc ttcttgggtg
    21121 ctcaagctgt atttgcggct ccaaccataa agattggtgt cgccggcgcc cactccgggg
    21181 acctggcttc ctatggtcta cccactgtca atgctgccaa gctggttgtc aaagaataca
    21241 atgacaaggg tggcattaat ggccaaaaag ttgagctcat tatcgaggac gatcaatgca
    21301 aaccagagat tgcaaccaac gtagccgcca aactggtaag cacaaaggtc gctgcggtca
    21361 tcggccatat ttgttctgga gctaccaaag cagcactggg aatttataag gattccagat
    21421 tagtgtgcat ctcgccatcc gcaacaacac ctttccttac ccagagtggt gaatatccga
    21481 acttcttccg cacaatcgcc ccggatgatg cacaagcaag gcttgcaacg gactttcttg
    21541 taaaggatct caaggtaaag aaaatagcag ttctccacga taagggcgac tatggcaaag
    21601 gattcgcgga gtctgtaagg gagtttgctg aggaaatggg cgtcaaggta gcacttttcg
    21661 aaggcatcaa tcccggtgct ccggactatt ccgctgtaat caataaagtt ggcaacgcca
    21721 aagtcgatgc tgtggtatgg ggcggatatc accctgaggc ttccaaactc gttcagcaga
    21781 tgaaagagaa gggaataaat gtagctttta tttccgaaga cggcataaag gacaacacct
    21841 ttatcgaagt ggctggaaaa tacgcggaag gcgtctatgc tactggccca accgatacct
    21901 ctgccaaccc aatggccaac aaggcgatcg aagctcataa gaaagaattc ggttccgagc
    21961 caggagcctt cttcttaaat gcttatgctt cagcccaagc gctgctcaat gccatcgaaa
    22021 aggcaaaatc cacggattac gataagatcg tggcagcttt gcagtcagaa tacgtagaca
    22081 cggtgcttgg ccggataaca ttcgataaga agggagatat agtaggctat ggtaactccc
    22141 ttttccaagt caagaacggt aagtttatag tattaaagta aggggcgagt tcttctgaat
    22201 cttcagaagc cgttctggca taagagtcgg aacggcttct ttttccaatc ctgctgtcga
    22261 ggatcggtac atggaatatt ttattaagct ttttttaagt ggtacagcaa aaggtagcat
    22321 ttatgctctc atggcattag gctacaccat ggtatatggc attattcagc tcatcaattt
    22381 tgcccatggt gagatctata tgatcggcgg tttcaccgcc cttattttag ggggattctt
    22441 cttttccaag ggcatgcccg tcagccttgt ccttgtattt tcaattctgc tttcgataat
    22501 atatgccgcg gcattcggct ttacgatcga gaaaatcgca tacaagccgc tcaggggaaa
    22561 accgcgcttg tcggctttga tcagtgccat cggcgtctcg ataatacttc agaattttgt
    22621 gcttctttca cagaccgaaa aatttcttcc atttcccaat tatcttcctg aatttagctg
    22681 gctattgccc tatagaagct acataaactc cactcaactc ataatactca taaccacagc
    22741 cataataatg gttctgctga ctttgctgat aaaattcacc cgtatgggaa aagcgatgcg
    22801 cgccaccgca caggatatgc acatggctca attagtgggt gtcgatataa atcaagttat
    22861 ttcgctcact tttgtcattg gctcgtcgct tgcagccatt ggaggggtac tcatctgctc
    22921 ctatatggga cagataaact actacatcgg cttcattgct ggcataaagg ctttcgttgc
    22981 cgcagttctt ggaggtatcg gaagtatccc tggagcggtt ctcggaagtt ttgtcctggg
    23041 gtggacagag agcttcggta cgggctatgt gtccagcgat tatgaagatg cctttgcttt
    23101 ccttattctc ataatcatac ttctcataaa gcccgatgga attcttggtc gttctcgacg
    23161 acagaaggtc tagactatga aagcacgctc tattcttaaa gatattggaa aatcactcat
    23221 tatcgccgtt tggttcatga ttttattttt ccccatcatg gttatgaaag tcaatgtgac
    23281 aaggggagtg gctcatatca atttccgctg gaatgtcctt cctctcattg gaattggagc
    23341 attcgtgctc tcattcgtct ggaccaaggc tctcgagtgg aatgaaagac ggacaagcaa
    23401 aaaaaagatc gagaatggcc ttatcaataa ccgtgcccta aatggaagat accttaaaaa
    23461 ccctgcgctt cgcccgctaa tccttctgtt agtctttgga tttaccgtgg cttttccctt
    23521 tctttttggt atgtaccaca ccaatgtcat gatcaccgca ttcatatatg tgatcttagg
    23581 tctaggcctc aacatcatcg tagggctcgg agggcttctc aatctaggct atgcagcgtt
    23641 cttcggcatt ggagcttata cttatggcct tatatggaaa tatatcgggc cttctttcat
    23701 cgcggcgggc ttaaatccag gctggctctt ttggatatct cttcccctcg caggcattgc
    23761 agcgacgatc tttggcgtaa tattgagtct gccggtattg agacttaggg gcgattatct
    23821 tgcaattgtc acccttgcgt ttggtgagat ctttcacatg gtgatgcaga atgccggcga
    23881 tctaacagga ggtgctaccg gcataagcct tattcctcgt ccctggtttc tcggtcaaaa
    23941 actgcagccc tcagaggctg ccacgtatat atactttata gctcttgtct tcgtaatgat
    24001 taccatattc gttgtgagac gaatcgaaga ttcaaaggtc ggaagggccc tcgaagcgat
    24061 gcgcgaagat gaggtagcct gcgaagcgat gggcatcgat atggtcaaga acaaattgat
    24121 aacctttgcg gttggcacat tctgggctgg aattgccgga gtgctgatgg ctagccagac
    24181 cacctatatc aatccagata gttttactct gtgggaatcg ataatgatcc tgatggctgt
    24241 tgtcataggc ggaaccggct ccattccggg agtaatcgcc ggggcaatac ttctaaaact
    24301 cctgcccgag tatttcaggc cccttgcgca ataccgcatg cttatatatg gcattgcaat
    24361 gatcctcgtc ataatcttta agtccgatgg cctaattcct cgcaagcgca agcaatacac
    24421 cttcacagaa aaggagcttg caaaatgagt acggagaagt tgcagtacta taatgaaatc
    24481 cttgagatag acaatctcgt catggctttt ggaggactgc gtgcaataga tggagtatcg
    24541 atgcacattg acgaaggaga aattgcagct ctaatcggcc ccaatggcgc gggaaagacc
    24601 acgattttca actgcataac aggtgtacac aagcctacag aaggaaaggt caagattcga
    24661 aatcgagatg gacgcgtcga ggaaatacaa ggcttaaagc ccaatgtcat caactgcaaa
    24721 ggcttagccc gaacctttca aaatatccgc cttttcaaca atatgaccgt cctcgaaaat
    24781 gtcatgattg gccgccacaa ttcgctcaag gcaggagtct ttgcagcaat aatccgcaat
    24841 gccataacaa gagaagaaga gcaaagagtc atcgaggaga gctacaccat tctcaagaaa
    24901 ctcaaactcg atacgtatgt caatgaaacg gcaaagaatc tgccttatgg cgaacaacgg
    24961 cgactagaga ttgctcgcgc tcttgcaaca gatccttttc tgcttctact tgacgaacct
    25021 gttgctggga tgaacgccca ggagacaaaa gaacttgagg agaccataaa tataatccga
    25081 gaccaggaac atataactat tcttcttatt gagcacgaca tgagccttgt tatggatgtt
    25141 agcgagcgta tctatgtatt ggactatggt aggctcattg ccgagggaaa tccgcacgag
    25201 atcaagaaga atcccgatgt catcaaggca tatctaggag aataaaatgg cgcttcttga
    25261 acttaaagat gtccgcactt tctatggaaa tattcaggcc ttaaaaggcg tttcgatctc
    25321 agtagaagaa ggcgagatag tcacacttat aggagccaat ggagcaggca agacgaccac
    25381 tctaatgagt atctgcggta tcgttcccat acgctccggc gagattttgc tcgattgtca
    25441 agatatttcg agaatgagtc cccacaagat cgtccagagg ggcgtctcac aggtgcctga
    25501 aggccgtcgc atttttccgc agcttacggt ctcagagaac ctcgatatgg gagcctttct
    25561 tcgaaccgat agagaaggca taaagcgaaa catggaagag gtctttacaa tattcccaag
    25621 acttgcagag cgtcgaaacc agctcggtgg aacactttcg ggtggcgaac agcagatgct
    25681 cgccatatcg cgtgccttga tggcaaaccc gcgccttctt ttgctcgatg aaccatcgct
    25741 ggggctggca ccgcttattg tccagaacat atttgaggtt atccaaaaga tcaacaaaga
    25801 gcgcaatacc acaatcctac tcgtagaaca gaacgcaaat atggcgctca agatcgcaaa
    25861 taaaggatat gtcatgcaga acggccttat caaaatcaca gataccgcag cagatcttct
    25921 caagaatcaa gaagttcgca gggcatattt ggggctttga cttactatca atatatttat
    25981 ggatgtataa tcgctgtagt ataataatag gagaaatatc gaaggaggcg aatatgaaag
    26041 taggtcagcg catgacacga aatcccgtta ccattacacc ggatacgact gttcctgaag
    26101 cgcaagctat tatgcgacgc gagaagatta gacgattgcc tgttctcgac aaaaacggca
    26161 agcttgttgg tattgtcagt gctctggacc ttacacatgc atctccttcg cctgccacaa
    26221 ctctcgatgt atgggaaatg cactatctgc tatcgaaact tacagtcgat aaagtaatga
    26281 ccagagatgt cataacgatt accgaagacc ttcctatcga ggaagcggca cgcataatgg
    26341 atgataatca gatatccggt attccagtaa tgcgtggcga aatacttgtt ggcattatca
    26401 cggaatcgga tatattcagg ctttttatcg agctttttgg cgctagacac aagggcatca
    26461 ggctaaccct ccttctacct gagagaaagg gagaactggc aaaagtttcc aatgcgatta
    26521 cgaaatgcgg cggtaatata gtctcttttg ccaccttcga aggagaagat ccaaccaatg
    26581 cctactgcac aatcaaggtg acaggcgtcg aagaaaaaac cttgatcgaa gcgattactc
    26641 ctgttgttca aaaagtcgtt gatgtcagag aaacttaagt tacggcgcca atagggctat
    26701 ctttaacact gaatcagcgc tggcgtttag agccagcccc tctttctcgc cacgatttct
    26761 ttgcttcgat ctttccccgc tgccattcca aaagagaaat gcgctcgaaa tcaataatga
    26821 tgcccaaatg ccttataaaa tcatcctcaa tcttgagttg ttccttcaag ctctccagat
    26881 tggctcccct tcttcttatt tcttcgagga tatagaaaag ctctgtaatt gtgcgagcaa
    26941 gtctatccag agaagccaca aagagcgtat cgccattgca aagactatcg agcgcacgtc
    27001 gcaattcggg cctgtctgct tcctttgaga ggggtttttc ctcgaatatt gattcaacgc
    27061 cagcttttct taataactcc aattgtgcgc cgatgtccgc atcgataggt gaaggtctaa
    27121 tatagccttt tcttaccgcc attctctcag cctccattaa caatccatac tgatacgaat
    27181 cttgtgtata aagcaagcag acagcaagaa gaatacttat gactcatggt taaatacagc
    27241 gaaggaatta ctatattatt ccccttcgat ttctttattc cttatttgct ctattctccg
    27301 ccattctgct tcattgtcat acacaaggtt cgtagcagta atgaaagctc gagttcaaat
    27361 tgcaaaccta aaatttcaat tgatattgat tgataaacaa taaaatatat gataaaaccc
    27421 aaatggccaa taattaaatg agagtcttat aaggatacaa agaatgatcc gccttttgaa
    27481 aatagcactt gttctccttg gagtcccagt ttttattctg agtgttttag ggttattttg
    27541 gctagccaat aatccagcga atccaagcta tgcttatatg ctatacccca ttttggttgg
    27601 catatattta tcaacgatac cgttttatat tgcactgtat caagctttta agatcctaat
    27661 caatattgat aaagacaatg ctctatcaaa acaatgtgca agagctctaa agaacataaa
    27721 actctgtgca atcgcagtca gtattttata tacgggaata ttgccattag tctttttcat
    27781 agcacaaaaa gacgatgccc caggcctcat agtatttgca accattccca tctttatttc
    27841 attggtgatc gcgatctttg ctgctgtctt tcaagcatta tttggaaata tcatggatat
    27901 gagcctagat aatgatctga ttgtcttaga cggacagact attaagcggt aatttggcgc
    27961 ccaatgacta tgtgtacgat aatgaaacag aaagccggaa aatagagcaa ataagaaata
    28021 tagaaaccga atgagataaa attataatta ctactttgca attcaaagta ctttgtgata
    28081 tactgcttat ataatgggca atttgggagt atcaactcca aaggagcgca gatcatgaaa
    28141 gaagaacctt ccataagtcc agatcaacag ttggagctca tcgagcgcat gctggaagaa
    28201 tctcgtaaaa ccgttctttc aatgggattc ccctcaatga tatggggagg ttttgtcagc
    28261 ataggtaccg cattgagcta tgcctttgca tcatggcact ggtatgtagc catagggcta
    28321 ttatgggctt tacttatggt aagcgctttt ctattagtgg ttcggcatga cagacggaag
    28381 caacagcagc agctaaaaac cacatcactc cacaaaatct ctaatgagat gtggatcttt
    28441 atctttggtg tgatggtact ctatggaata gtcgggtttt tgcgccccca gaatatctct
    28501 cttccctacg ccctttgtgt tgtatcgctt ctcgttggca ccgcctattg gataagcagt
    28561 agcatgaccc gctattttct cttgcgaata atgagcgccc tttggattgt gtgcagtatt
    28621 gttgttttac tggtaccagc cctttggtcg cctgcagtgg tgggaatatg cgccttctta
    28681 ggcgaatgta tgccgggaat ttatttctat gcaaaagaac gaaaagaatc cgtataacta
    28741 tcagagcttt gatgaaatgc tttcgtctag gatacgcctt gcagcacttg cattgcttgc
    28801 tggctgcgat gaagctgaat ttacttatat tcgtgatgtc atccaaacca ccgatggcaa
    28861 tctggcaagc catttgcgaa agctagaagg aagcggatat gtgcggtatc aaaagcgatt
    28921 catcgaccgc aaacccgcta catactatcg aataactcca caggggagat ccgctctttt
    28981 ggcatatagt cgtcgattga ccgaattatt gaagaattcc aattaccaag gaggttcatc
    29041 aaatgagact gaaacagatg agaagtaaaa ggcctaggag ccatgtcgga tcgacaactc
    29101 gggctccttt gtggccttat gaaagtgctc aattcagacc acatcgcaaa gtaatctgtg
    29161 cggtgctcgt ggtagcgctc ttggggatcc ccgcgaccat gcttttctct caagaccagc
    29221 atcctaacaa attgcctgcc aatacattgc ctcttgattt ttccactcta gaaggcgcta
    29281 ctgcagttga ccacaacaag cacaggtctt agagtgcacc tataggagct taaaatcaga
    29341 tggatagacg ggcaacgagg tccaggacgg cttgctcgat ctc
//
LOCUS       DAOOWY010000014        29239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1605_length_29239_cov_10.314933, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000014 DAOOWY010000000
VERSION     DAOOWY010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 29239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29239
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1605_length_29239_cov_10.314933"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PK105_02265"
     CDS             <1..798
                     /locus_tag="PK105_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU60562.1"
                     /translation="PIIGSFYLGFTDWDLLRTPKFIGLSNFSEALSDPGLIRIVVNTV
                     LFSVIYVIGVMVIGLFLALMVNQKLPGTNFFRAAYYLPVITSAVAIGIIWSWILSPQV
                     GILNILVKSVGLKPPNWLGDTHWALLTVSVIQVWKMAGYYMVLFLAGLQSIPDSVKEA
                     AVVDGANSLQLFFHVTLPMLSPTTFFVLTVAMIDSFKNFELIYTMTRGGPQSSTTTLV
                     YDVYVYGFVHYRMGYASAVAYVLMVLVGAITIANFYIKKRWVTNSYY"
     gene            808..1641
                     /locus_tag="PK105_02270"
     CDS             808..1641
                     /locus_tag="PK105_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU60563.1"
                     /translation="MKYVSHIILYILLIFFALLTAFPFFWMIVNSFSSEAGIFRVPPQ
                     LIPDLIGTNQLWSNYAVIFQQFHFGRYFLNSLFISGASAIGQVIVCSLAGYVFALMNF
                     RGKRLIFSALVFTLMVPAQVTIIPEYFLMMKLNWLDSYLPLIIPSFLVGSFGTFMFKE
                     FYETFPSSISDAGIIDGAGAFRMFTRIYLPQSGPQAATLFIIAFMNSWNDLLRPILYI
                     SDDSMQTVTMALTQFQSQYTARWNLLLAGGVISVMPLLLIFVFLQRYIIENSINSGIK
                     G"
     gene            1638..3002
                     /locus_tag="PK105_02275"
     CDS             1638..3002
                     /locus_tag="PK105_02275"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOU60564.1"
                     /translation="MSESSQKATVSVSERRYKNGDLARYEIVNFESWEHLYRVFGRTD
                     ADIVKEITRLFQANILPGKTTIGPVILFAVPEGTIDPFQTETEIGFIVNQERRIKYWL
                     TKALARGEFRIEKGNYIFDNSKYESFWESLTHAGMTGIAKINRGYPVLLPLYRDLGTL
                     IDHSKNSIVFNSHFFQMETTDCDTPWDIMGEPFGLLILDGKILLPPLFEREAFFIGKT
                     GNSWIGRPSLRDVEVYIGSTGYIDGYNCRFFSRPAIDRTSKSEGTDLIIVGKHVVGLH
                     DGGCTEIPEGGFIIQTYKRCYPDSFDVDYLGFNNVAFAVQVGPALVREGKAAVGFGPS
                     PFWNGLGPTFPPSVYPLDWERGRAARLGLGSKDGGLLLIWIEGSNKLSYRKGVDSCGA
                     SLAEFAQICIESGVKEFVSLDGGGSAQICIDGGRLLRLSDRYPHSGKDAERPLPFGLA
                     FDLV"
     gene            complement(4106..5062)
                     /locus_tag="PK105_02280"
     CDS             complement(4106..5062)
                     /locus_tag="PK105_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU60565.1"
                     /translation="MNMNKLHLSKSKTRYIIVVFLAILSLSIHPLCAQNQCSVPGLPT
                     IRIATWNVKDCSATNQKTKEAFLLHSFIAEAIKEARIDIIAFQEIQIEGRKGADIALL
                     QDALMSIDWAMPHVAWARSPLNDDLAILSRFPITESQTILAPSEQPWPRPVLEACIDL
                     GDMLLYLYTAHFKAYADQESLSARLAQAQALASHIRQYFGQSIKETAIILAGDFNTVS
                     SYDMQSKIGTVDLLQLRDNEDSTDDFLSLNLEWLNFKPTYKSKSYASITDHIIVSPLL
                     SAYADRDSIEIVDPLLNENGLSLSDHKMLVLNLDRRWLVIQK"
     gene            complement(5146..6291)
                     /locus_tag="PK105_02285"
     CDS             complement(5146..6291)
                     /locus_tag="PK105_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60566.1"
                     /translation="MKKRLSILSIVLLFASLTTAFAKPGINFSVNSIKTNSDIIAGIP
                     IPSGADIGLEIPIGSGLYSVTFRGAGGYESRMILRNPTTSVPIMAPSAIDNTNRFFWT
                     NAMAELGVRRYLVRKDDGGKSIWLFGLARGRYEDNATSFPTTVFADAQGMQSISAMLG
                     IVHDSTFWQEPNRKVGWYAEFSTEYSPAFANFSGSPMDFGRFNLTAEGFIPLTPEAGT
                     LRNPSMMAPYIVLYGVGDYAVGSHIPHQILTTFGGIKGTKGLGDMVRGAQPWGYEAPI
                     KGYASVELRFPDQSILESFALYPIGYVFGDIGAYGGLYNNPLGTTFIDNSGVIASAGA
                     GVSLSVQNYLWLGAYAGWRFPIIDPLASTYYSKPSGFFWGITFAAHY"
     gene            complement(6380..>6950)
                     /locus_tag="PK105_02290"
     CDS             complement(6380..>6950)
                     /locus_tag="PK105_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOU60567.1"
                     /translation="LCTRGSVSDSETSVPHTSLPQDSASLGFSPLPHTSVPRDYRDLP
                     QRYDFAPLSWCIPYEYEPVSKLLVVCDTSHKTFHDIEENRVFAIAFPSFEMRALIQRA
                     GSVSGFDVDKFDHFNVSCFCAKTIDVLIPEGVVGWMECSLERIIIEGTSGILLGAVIK
                     GFAMPESWKRRIHYLNDSIWYKPGDLISD"
     assembly_gap    6951..7050
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7307..7942)
                     /locus_tag="PK105_02295"
     CDS             complement(7307..7942)
                     /locus_tag="PK105_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOU60568.1"
                     /translation="MLTYVLVGFALAADAFAVSVSAAACATALPLLIGIRAAFMFGFF
                     QFLMPLVGWSLGSAFSALIQNYDHWIAFGLLIIVGSKMLYEAIHMRRESACDDPEKVV
                     AAKSKTIAATNKGTNSASKGIMKLNTLLVLAFATSIDAMAVGLSYNLLGMPIMLPSFI
                     IGTITFIVCIIGIEFGKRLRKLIQEWAEIGGGSILIIIGFRILIDHIIKGT"
     gene            complement(7981..9153)
                     /locus_tag="PK105_02300"
     CDS             complement(7981..9153)
                     /locus_tag="PK105_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOU60569.1"
                     /translation="MYNFDTIVDRSNTYSIKWDRDQLPEGCRDAIPMWIADMDFACSP
                     DIVKAIQARAEHPIYGYTKCSEEYYKSFIDWMKHRNGWSIEKDWILFSPGVVPAINLA
                     VLAYTQPGDKIIIQPPVYNPFSAAVLNNSRQLVENPLVIRDGRYTMDFEDLDRKIDTQ
                     TKLLILCSPHNPVGRVWTIDELSKLIDICSSHNILIISDEIHSDIILGTEVHHCTASL
                     SEKAAAITITLTAPNKTFNLAGLQIANIVIPNRELSKAFGEQATKIGINLTNTFGMVA
                     QEAAYTKGEPWLEELLSYLKENYATLKSFIDRRIPSLKVFPLEGTYLPWLDCRVLGLS
                     DAELHDLFLKKARLWLDDGTMFGTGGSGFMRINIACPRNVLIKALEQLENAIKTNA"
     gene            complement(9165..9545)
                     /locus_tag="PK105_02305"
     CDS             complement(9165..9545)
                     /locus_tag="PK105_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU60570.1"
                     /translation="MEKECIQAKTAPAAIGPYSHAIRAGALVFCSGQLGIDPSTGDLV
                     EGIEAQTNQALRNLVSVLEACSCTVANLVKTTIFLKDMNNFPKVNEIYAGYFDGDFPA
                     RSAIQVAALPKNALVEIEAIAYKQ"
     gene            complement(9734..10282)
                     /locus_tag="PK105_02310"
     CDS             complement(9734..10282)
                     /locus_tag="PK105_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOU60571.1"
                     /translation="MKTACMLLAEGFEEIEAVTPIDYLRRAGIYVNVVGIVGKRVRGS
                     HGIIIETDSGPEALEKDYDAIVIPGGMPGASNLANSALVRSLIIRHYEKGKLIAAICA
                     SPAIVLHGACNLLQGREFTCYPGTEDQIRGGHFRAERVVKDGNIITSQGPGTAGEFAI
                     SIISVLEGPQKANEVAQHALLK"
     gene            complement(10337..11119)
                     /locus_tag="PK105_02315"
     CDS             complement(10337..11119)
                     /locus_tag="PK105_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOU60572.1"
                     /translation="MLTRDLDRIREAYKRRHPEATRRAYNPSHPHQAEEKHTTTQDKH
                     IKSIRYSGFDGTITTFTAASGVACASLSSGIVLMIRLADLLADGLSMSISYYLSSKSE
                     TEYEADRRARDEWEVENYPEGEKFELKEIYMSRGVSQKDAKPAIEITAKGKTALVDTM
                     MIDELEIILSDESRLANAIATFISLALSGLNPFSTYVFVLFVPSLDSIRFLFACILIL
                     ARIIMFILGSLKIILIERTYSRAALKCCWLDVSQLLLPISFV"
     gene            complement(11451..12752)
                     /gene="eno"
                     /locus_tag="PK105_02320"
     CDS             complement(11451..12752)
                     /gene="eno"
                     /locus_tag="PK105_02320"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOU60573.1"
                     /translation="MSIIEYIEAREILDSRGNPTVEVDVVLEDGTLGRAAVPSGASTG
                     EYEAVELRDGDKKRYQGKGVLAAIENVNTIIAGEICGLDSLEQVDIDRTLIELDGTEN
                     KGRLGANAILGVSMATARAAAEYLGLPLYKYLGGVHTNLLPVPMSNIINGGKHADNEI
                     DFQEFMIMPLGAESFREAVRMNAEVFHALKSILKADGHNTAVGDEGGFAPNIGNEDAL
                     DYIIRAIEKAGYKPGEQIAIALDPAASELFEEGDGKGYRFWKSSPDRLFSSDEMIELY
                     TNWINKYPIVSIEDGLDQNDWEGYVKFTKALGNKIQIVGDDFFVTNTKRLAKGIEMGA
                     CNSILIKLNQIGTVTETIETINMAKRAGYSAIVSHRSGETEDTFIADLVVGLETGQIK
                     TGSMSRTDRVAKYNQLMRIEDEMEGIAAYWGHKVLNAVYRP"
     gene            complement(12896..>13911)
                     /locus_tag="PK105_02325"
     CDS             complement(12896..>13911)
                     /locus_tag="PK105_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60574.1"
                     /translation="EGRRLKAEILTEGRRLRASQAVIRFFVLITIFLLILLANLPAQQ
                     PPSSSSFQGIKDISRINSPLPPSFNAVLPKCISFQPGSPILRTSEHIGGELLLAPSLD
                     GVSFIIEHIKAAAKESNLIIEALIFLPNSSVEGPSSISAQLDSLGLIFNQFSSIQGIQ
                     YWSASRKIMRTLYVESYRIDNLKSRNRISDPSTASELKALLSKPMYLHQKDQTFDALI
                     LEAHCSITNSYFLMTNKNVTPVRMIGIPVLPINGLRTGFFAAPSPNGTNGILLYFVTS
                     VQSPSIARDRVFESASNKALALLHWFIEKAFSQKLIDPVTLPWNFDDMPADVRVSAIP
                     GKI"
     assembly_gap    13912..14011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14092..15003)
                     /locus_tag="PK105_02330"
     CDS             complement(14092..15003)
                     /locus_tag="PK105_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOU60575.1"
                     /translation="MAKFGLGKGLGALIPEHQQFFEQSLEETSASIVKLVSIEDLIPN
                     PDQPRKSFTQESLDELAESIRRHGLLQPLLVHQEKSGKYIIIAGERRYRAAQIAGLEQ
                     LPVIVRSQDDENHRIELSLVENIQREDLDPIEEAQAYAKLMEITGVTQERVAEMVGKS
                     RVAVANTLRLLKLPEDMQLLLKQGIISAGHARALLSIEEEKERRKLFEHIQQNNLSVR
                     QAEQEAQKIQNALRGQRTKSKKQLESLKTIEEIPLDPLLKELMEKLIERLGTKVEISG
                     SIDSGTIKIHYFSQEDLERIYEVLKIE"
     gene            complement(14996..15751)
                     /locus_tag="PK105_02335"
     CDS             complement(14996..15751)
                     /locus_tag="PK105_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOU60576.1"
                     /translation="MARIIVFVNQKGGVGKTTSAINIGTSLALQYKKTLLIDFDPQGN
                     LTSGIGGKLSHTNIYHVISGLAEIQQIIQSVPVPRLFLAPSSVDLSGATIELVDRPDR
                     NDYLRRAIAPVVNDYEYILIDCPPSLGVLTLNGLAAATEVIIPLQCEYFALEGLSLII
                     QTIGLVQKSINPSLRINGILLTMFDARTRLSQEVVRQVTEYFKDKVFKTIIPRNVRLS
                     EAPSHGLPAIMYDPTCIGAKSYEAVTKEIINRG"
     gene            16016..16237
                     /locus_tag="PK105_02340"
     CDS             16016..16237
                     /locus_tag="PK105_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOU60577.1"
                     /translation="MFEQVKSAIENVRPSLKADGGDIELVSVSDDGVVKVRLTGACGA
                     CPYSMMTLKQGIEAYLKKAVPQVKEVKQA"
     gene            complement(16336..17106)
                     /locus_tag="PK105_02345"
     CDS             complement(16336..17106)
                     /locus_tag="PK105_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOU60578.1"
                     /translation="MADMKVQSLDRAFDILEILAREPTGLTLVEIAAESDLPRSTTFR
                     LLSVLLQRDYVRKGPYDNFYRLGPGFIELSSNYLNSLELKTESAPFMRELASSLGTIV
                     FLARRQGNMMVYIDKEDQFTSLRKYAIIGEQRPIYCTSLGKALILDMDDDEIRNLIPD
                     TFLTKFGPNTHIDVESLIKDIHICKARGWTHDNEEAEPGVNCVAAPIRDYRGQIIASI
                     STSWLLEIRPELEAEKVALFVVRAANEISRTMGFYENL"
     gene            17297..18127
                     /gene="kduI"
                     /locus_tag="PK105_02350"
     CDS             17297..18127
                     /gene="kduI"
                     /locus_tag="PK105_02350"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOU60579.1"
                     /translation="MEIREAINSEWAKKMGTEDLRNAFLVEKVFIVGEMTAIYSHVDR
                     MIIGGIVPTSVPLALPVSKELGTEFFLERREIGIINIGGEGFVEVDGTIHDIGQRDAI
                     YVSMGSKNVAFSSKNPKEPAKFYFNSAPAHRACKTRLITLDQANHVALGSDEECNKRV
                     INQYIHPTVLETCQLVMGMTTFSEGSVWNTMPVHTHERRMEVYLYFDMPDDKVVFHLM
                     GKPQETRHIVVRNEQAVISPSWSIHAGVGTGKYTFIWGMAGENQTFDDMDNVEMADLR
                     "
     gene            18156..18917
                     /gene="kduD"
                     /locus_tag="PK105_02355"
     CDS             18156..18917
                     /gene="kduD"
                     /locus_tag="PK105_02355"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HOU60580.1"
                     /translation="MILDEFRLDGRVAIVTGSSTGLGQGMSIALAEAGADVVGVDYVP
                     SKETASIIEAQGCKYLEIVANLLTIEPIQSILDKTISAFGRVDILINNAGTIRREDAI
                     KFNEKDWDEVMDLNVKTVFFFSQAVARKYIEQGTGGKIINIASMLSFQGGIRVPSYTA
                     SKSAVMGLTRLLANEWAKYHINVNAIAPGYMATNNTAELRADRERSAEILSRIPAGRW
                     GTPEDVKGAAVFLASPASNYVNGYTIAVDGGWLAR"
     gene            19060..19506
                     /locus_tag="PK105_02360"
     CDS             19060..19506
                     /locus_tag="PK105_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOU60581.1"
                     /translation="MLSDLSRYRGLSKNLDVAFDWLHKGLWRQLSIGKHSVDGDAVFV
                     LIQEYTAKEHSACRFESHREYIDIQMMISGKEIIEVLPANELNVIEPYKPDIEFYASP
                     TDRNANVLAMSAEQLAIFFPEDAHRPCMKAGTLAEPVKKVVVKVAI"
     gene            19800..20678
                     /gene="eda"
                     /locus_tag="PK105_02365"
     CDS             19800..20678
                     /gene="eda"
                     /locus_tag="PK105_02365"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOU60582.1"
                     /translation="MVKKEKIGELFYKTGLIPVIKIDSEEMTDGLAKALRGGGLSVAE
                     ITFRTKAAPKVIERFASCHEDIIVGAGTVTKKDEVDAALLAGAQFIVSPGFNPSICEY
                     CLGKDVAVFPGVNNPSLIEQAMAMGLSILKFFPAEVSGGVKALNAFKSVYQQISFIPT
                     GGIQENNIHEYLSLKNVLACGGSWIVSADLLEARKFDEIERLVKSARRAMMGFLPIVQ
                     DGIIDEENQIRSMRDGGCIGGCIEVKTPSIKRTLAMLSYLKAYVVPGSEVEKGQGLVE
                     VSISIPGLHTQIHLVE"
     gene            20693..21718
                     /locus_tag="PK105_02370"
     CDS             20693..21718
                     /locus_tag="PK105_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOU60583.1"
                     /translation="MSITTFGEIMLRLKSPGQERLLQSPFFEATFGGSEANVAVALSR
                     LGITARFISILPDNPIGRGCMRELRSHGVDVSCLKFKKGRLGIYFFENGANQRPSNVI
                     YDRENSAISRIAEEDIDWEASFKDMQWFHVSGITPALSQNCADETIKAACIAKDRGLT
                     VSFDLNYRAKLWNYGVDAYSVMCKIAQYADILIGNEEDYQKSLGIKGPSAIGAQQLDI
                     DAYQEMCETALKQYPNAKIAAVTLRESYSADNNDWSAMIVSEGRRFISRKYHISDIVD
                     RVGAGDSFVAGLIYGLITKKDYQAALEYATALSCLKHSISGDFALLEKGEVEKLLGGD
                     ASGRVQR"
     gene            complement(21773..23101)
                     /gene="prs"
                     /locus_tag="PK105_02375"
     CDS             complement(21773..23101)
                     /gene="prs"
                     /locus_tag="PK105_02375"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOU60584.1"
                     /translation="MSYSDPTHLGIIACPGAEAFANQVIRKLSGIYKHRFMRKAQAIA
                     GRYHITPDLVIRKINFANDCNTGMLFLPGDKERYRPPKFKINVRHTYFANGEIKTEIL
                     ESIRGRSIYIFQDVENHQLVLFNDGTIQKVLSVNDHIFNLFVAIDAAMQAGAAEINLV
                     LPIYPYSRQHKKKGREGLTAARIGKMLESLGVNRIITLDIHSKEIENAFDRLRMENLH
                     ASFQIIEKLTSIIDIAKEDIVVLAPDTGAVDRNKFYANALQKPLALLYKERDYSKITK
                     SATETNISEMRLLGNVSNKVVFMADDMIGTGGTLIKAMRYLKELGASKVICAVSLPIF
                     SANSIDDFDAAYKEGLFYRVIGTNAIYHNELLKKEWYIQSDVTGLFAQVISLLHHDRS
                     LSSLLDNRDVVARLIKRKLESTSSSRTSPNQPKDENANEDVVPYGAANKP"
     assembly_gap    25057..25156
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25255..26907
                     /locus_tag="PK105_02380"
     CDS             25255..26907
                     /locus_tag="PK105_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU60585.1"
                     /translation="MIHRNSNIDTESLAIRLARFSSITRTSGELSYPDFLISVLREIS
                     FFSNRPNLVQEFEISEDKWKRRIVIAFAPGEGQACITLTGHYDVVDTSNYGALEPLAF
                     DPEALLKKMLQVLSAKEGLSSSEISLKKDLESGYFIPGRGILDMKSGLAAAITVISRF
                     LEDPDRIGNIIFLAVPDEEGASVGMKAAKKILSDFIISHQLQMRAIINLDAAVDQGDG
                     GEAKAVFLGSVSKLLPFVLFVGKAAHAGAPFDGFNPALAASLFIREIECNSDCFSPRA
                     ISPGEEPPPPTILYCRDMRTHYDVTMPFDIFCAVNILTFEKKPEKIFESFRLLVEEML
                     NNAISLFHERKSVFSRKKNEHVAMQRFSSRVIDFSELVDRAEYVAPGILDRLRKAAEK
                     YHPEDPVLQTARIVHELLSFAQIEGPCAIIGFAPPFYAKAELDIYRDQEFLVLLKDEI
                     AQFNREYQETIKLRPFFPGISDMSFLAPSIESHAIEYIRKQSAVIQKDLDEYMDDLIE
                     APVINIGPWGREYHQWGERVNRHYAFKVLPDLVFRISQRILR"
     gene            26984..27715
                     /locus_tag="PK105_02385"
     CDS             26984..27715
                     /locus_tag="PK105_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOU60586.1"
                     /translation="MTIVISLGGSIVAPQEGPSGIFLYDFRNVLLSWINGGDHKAIIV
                     VGGGYAARQWQKAYKEMLEMDKAISRDGGSSISRNIDVRQSLDRIGIAATRLNAQLIR
                     EVFSDYCRDPIVTDPSADITMNGKILIAAGWKPGFSTDYDAVILAERFNAEKILNLSN
                     VPQIYSADPKVDPNAKPLFHISFDSLLKMIGTEWDPGANVPFDPIAADKARELGLTVI
                     FASGTNLMNFADILNDRAFIGTIID"
     gene            complement(27868..>29239)
                     /locus_tag="PK105_02390"
     CDS             complement(27868..>29239)
                     /locus_tag="PK105_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOU60587.1"
                     /translation="AWIADCWTNGGSQTKISKIRFFYYPARKTLDFLEQILSSFFLVY
                     SNRIYPASNGLDALYTLQEPDGAIRSAYDIETGAPIFPEDNPLGVTLPLLSWAEFNLY
                     HKTANKKRVKEIMPILSNYYRWLEANFKMPNGLFSTPLSSTGMINSPRSEARYFIDFN
                     ATMAMNALYLSALGDILNDKDASFQYKRDYFSIKTRINSKMWNPEDGFYYDLDENEIQ
                     VRTKTLGTYWLLLAEIPNEERAEKLIAKLKDPSCFGTDNPFPSLSVDHPAFDKKGQGF
                     RGSVFPWLTFMVIKGLEKYYFYELARNSAIRHLYYILDSMHNSDQEANKRSNHPTVWS
                     AYQPMNEGKAIWEGHPDWPLPNHFPSNGLSTVTLIIENIIGLYISLPRKTVDWIIPNL
                     EVMGIENLSLKRNMITILSSKSGRGWEIHMESEKLYYFTINVLGKKKKTLPIPSGKCS
                     MLIDKI"
BASE COUNT         8098 a         5679 c         6608 g         8554 t
ORIGIN      
        1 cccataatcg gttctttcta cttaggattt accgactggg atcttctcag aacaccaaag
       61 ttcattggtt taagcaattt tagcgaagcg ttgtctgatc ctggacttat taggattgtt
      121 gtgaacactg tgcttttctc ggtcatatac gttattggag ttatggtaat tggccttttt
      181 ctcgcgttaa tggtaaacca gaaactccca ggaacaaact tttttagggc agcttactat
      241 ttaccagtca taacctcagc agtagctata ggtatcattt ggtcatggat cttaagccca
      301 caagtcggaa ttctcaatat tcttgtcaag agtgtggggc tgaagcctcc taattggctg
      361 ggagatactc actgggcttt actcaccgta tcagtaattc aagtttggaa aatggcagga
      421 tattatatgg tacttttcct tgctggcctg caatctatcc cagattccgt gaaggaagca
      481 gcagtggttg atggggcaaa ctcactgcaa ttattttttc atgtcacctt gccaatgctc
      541 tcgccaacta cgttcttcgt gttaacggta gctatgatcg attcattcaa gaacttcgaa
      601 ctcatctaca caatgacgcg cggtgggcca cagagttcaa caaccacgct cgtctacgat
      661 gtatatgtat atgggttcgt tcactacaga atgggatacg cttcagcagt cgcttatgtt
      721 ttgatggtgc ttgtaggagc tattaccatt gcaaatttct atattaagaa gcgctgggtt
      781 acaaattcct actattgaga gaaatatatg aaatatgttt cgcatataat cctttacata
      841 cttctcatct tttttgccct tctaactgcg ttcccgttct tttggatgat agtaaactct
      901 ttcagcagcg aagctggcat ttttcgcgtg cctccgcagt taataccaga tcttatcggt
      961 acaaatcaat tatggagcaa ctatgctgta attttccaac agttccattt cggtcgttat
     1021 tttctgaata gcctttttat ctccggggct tctgcgatcg gtcaggttat cgtatgttcg
     1081 ctcgcgggat atgtctttgc gctgatgaat ttcagaggca aaagacttat attctctgcg
     1141 cttgttttta cccttatggt acctgcccag gtcacgataa tcccagaata ttttttgatg
     1201 atgaaactga attggcttga tagttacttg ccgcttatta ttccttcttt tcttgtaggt
     1261 tcgttcggaa cctttatgtt taaggaattc tatgagactt tcccatcttc tatctctgac
     1321 gcaggaatca tcgacggcgc tggcgctttt aggatgttca caaggattta tcttccacag
     1381 agtggacctc aagctgctac tctgttcatt atcgcattca tgaatagttg gaacgatctg
     1441 ctgcgtccca tcctttatat atctgacgat tcgatgcaga ccgtcacgat ggctcttacg
     1501 cagttccagt cacaatatac cgcaagatgg aatctacttc tggcaggggg cgttatatcg
     1561 gtaatgccac ttctcctaat attcgtattc ttgcagcgat acataattga aaattcaata
     1621 aattcgggga taaaaggatg agcgaatcca gccagaaggc tactgttagt gtatcggaaa
     1681 gacgctataa gaatggagat cttgcacgct atgaaatcgt caatttcgaa tcatgggagc
     1741 atctttatcg ggttttcgga aggactgatg ctgatatagt aaaagaaata acgaggctat
     1801 ttcaagcgaa tatacttcct gggaagacaa cgataggtcc agtgatattg tttgcagttc
     1861 cggagggaac tattgatcca tttcaaactg aaaccgagat cggtttcata gtaaatcaag
     1921 agaggcgcat aaaatattgg cttaccaaag cacttgctcg aggtgagttt aggatagaaa
     1981 aagggaatta catttttgat aactcgaagt atgagtcttt ttgggaatct cttacgcatg
     2041 caggaatgac tggaatagct aagattaata ggggttatcc ggtcttatta ccgctttata
     2101 gagatttagg aacccttatt gaccatagca aaaatagtat cgtgttcaat tcgcatttct
     2161 tccagatgga gacaacagac tgcgatactc cttgggatat aatgggtgag ccatttgggc
     2221 ttctcatctt ggatggaaag attttattgc cacctctttt cgagcgagaa gcgtttttta
     2281 ttgggaagac tggaaatagc tggattggcc gaccttcgtt aagagatgtg gaagtgtata
     2341 tcggatctac cggatatatt gatggttaca attgcagatt tttttcacgg cctgctattg
     2401 atagaacatc caagagtgaa ggaaccgatt tgataatagt aggaaaacac gttgtaggct
     2461 tgcatgatgg aggttgtacg gagatacccg aaggtggatt cattattcaa acttataaga
     2521 gatgttatcc agattccttt gatgtggatt atttggggtt caataatgtg gcttttgcag
     2581 tacaggtcgg accagcgctt gtaagagaag gaaaggcagc agttggattt gggccttctc
     2641 ccttctggaa tggtttagga ccaacttttc caccttctgt ctatccacta gattgggaaa
     2701 gggggcgagc agcccgttta ggtctcggat caaaagatgg tggactccta ctaatctgga
     2761 tcgaaggttc aaataagctc tcatatagga aaggtgttga tagctgtgga gcttcgctag
     2821 cggagttcgc gcaaatctgc atagaaagcg gagtcaagga gtttgtgagc ctcgatggtg
     2881 gtggatccgc acagatatgc atcgatggag gaagattgct caggctttcg gatcgttatc
     2941 cccattctgg caaagatgct gaaaggcccc taccgtttgg actagccttc gatttggttt
     3001 aattgggaag caaagctcgg caatctcggt ttgaaccgta ccagattcgc ttggcacaga
     3061 aaatctggag gaaatataat catcaacata gcacataggt cttctggatt ttattggcaa
     3121 gggagatatg ggaacgagca gtctcgctgc aaatgcttca atcggagcag actgaatgcg
     3181 aaaggacagc gataaacccg ataaccgact taacatatac tggtactgct gaatcattga
     3241 gataggggta tgctttatgg caagaatgat aaaacacctt gaaatactag gtaaaaacat
     3301 ccttgcacaa taggggggaa aacatttgct cagttaatga agcagttctt taaccttcac
     3361 atttttggag taataccggc tttttagcat ttgcgtctgt agagaatgat ggggaataat
     3421 aatccttggt gagcccgtca gatcgagttt aagtgttaaa acgataagta attaatcaca
     3481 atagatcctt taatagtgaa aattagaaca atagtagaat ttgattaaaa atgcttgaaa
     3541 acagctgttt attgacatgc aaaagctctt agataattaa aattgactta ggaatagtgc
     3601 agatactgcc atgcgaggca ggaggtcagg cttatccttg cgtcatatcg tgggtccgaa
     3661 tcccttgcca gcattattcc ttatattttg gatagatttt taaaacacca ggtgtaccaa
     3721 tgatgtttgc tcttgatagt attatgtaag aaattaattg ccatgcctga gattatccag
     3781 cccttctaaa tgcacctttt actagaccct cttttcgaga cgatccgctt tcactcttac
     3841 cagaatacct tttcataaat agataagtct tatttgttga gtcgaattac agcggcttaa
     3901 aataatcgta gactaaaagt cgcagagttt attctattct cagcaattgc tatgccaatt
     3961 accttattac tgatacaatt atcgataaca gaagaatata tgctttgcta tattttcatg
     4021 ccctctaggt taaatttgca ctagtgattt ctaacaaata tgctagacca tagcgacagc
     4081 gcaacattaa tattgggaat tcggcctatt tctgtatgac cagccagcgg cggtcgaggt
     4141 ttaacacaag cattttgtgg tctgataatg ataagccatt ttcgttcaga agaggatcga
     4201 caatctcgat cgaatctcta tcagcatagg ccgaaagaag cggcgagaca attatatggt
     4261 ctgtgatgct tgcgtaagat ttcgatttgt aggttggctt gaaattgagc cactcaaggt
     4321 tgaggctcaa aaaatcgtct gtcgaatctt cattatctcg cagctgaagc agatcgacag
     4381 tacctatttt ggactgcatg tcgtaggacg aaacggtgtt gaaatcgcca gctagaatga
     4441 tcgctgtttc ttttatcgat tgtccaaagt actgtcggat atgcgaagcc agcgcttgag
     4501 cttgggccag gcgcgcagaa agcgattctt gatcagcata agccttgaaa tgagcagtat
     4561 ataggtaaag gagcatatct cccaaatcga tgcatgcttc taggacaggc cgaggccaag
     4621 gctgttcgga tggcgcaaga atcgtttggc tttctgtaat aggaaatcga gagaggatag
     4681 caagatcatc attcagcggg cttctagccc aagcaacatg tggcatagcc cagtcgatgc
     4741 tcatcaatgc atcttgcaat aatgcgatat ctgctccttt gcgtccctcg atctggattt
     4801 cctgaaaggc aatgatatcg attcttgctt cctttatggc ttcagcaata aaagagtgca
     4861 acagaaatgc ttcttttgtc ttttgattgg tagcgctgca gtctttaaca ttccatgttg
     4921 cgatgcggat ggttgggaga ccagggacag agcactgatt ttgtgcacaa agaggatgaa
     4981 tggaaagaga gagtatcgca aggaaaacta ctatgatgta acgagtttta gatttcgata
     5041 ggtggagctt attcatattc attacatact ctccctgatt tggctgccga aaacagcgat
     5101 actctgccct ttctggtcta tgattttctg gtgcttaggt ttgcctcaat aatgggctgc
     5161 aaaggtaatt ccccaaaaga agccactggg ttttgagtaa taggtcgagg ctagtggatc
     5221 gataatcggg aaacgccagc cagcataagc accaagccaa agataattct gaaccgataa
     5281 agagacaccc gcacctgctg atgcaatgac tcccgaatta tctataaaag tagtacctag
     5341 agggttatta tatagtccgc cataagcgcc tatgtcgcca aatacatatc caataggata
     5401 tagcgcaaaa ctctctagga tcgattggtc tgggaaacgc aactccacac ttgcatagcc
     5461 ctttataggg gcctcatagc cccaaggctg agctcctcta accatatcac caagcccttt
     5521 cgttcccttg atccctccga aggtcgtcag tatttggtga ggaatatgtg aaccaaccgc
     5581 ataatctcct acgccataga gaacgatata tggtgccatc attgaaggat ttcggagtgt
     5641 tcctgcttct ggtgtgagtg gaataaagcc ttccgcagtc agattaaaac ggccgaaatc
     5701 catcggactg ccagaaaagt tggcgaaagc tggactgtat tccgtggaaa actctgcata
     5761 ccaaccgact tttcgatttg gttcctgcca aaaagtcgaa tcatgaacaa tgcccaacat
     5821 tgcggagatc gattgcatgc cttgagcgtc ggcaaatacc gtggttggaa agcttgtggc
     5881 attgtcttcg tatcgacctc gagcgagtcc aaaaagccat atgcttttgc cgccgtcatc
     5941 cttccgaacc agatagcgcc ggacacctag ttctgccata gcattggtcc aaaagaatcg
     6001 gtttgtgttg tcaatggctg atggggccat gatggggacg gaggttgtag gattgcgcaa
     6061 gatcattctc gattcgtaac cacctgctcc tcggaaggtc accgaataca agccagaacc
     6121 aatggggatt tcaaggccga tatctgcacc ggaaggtatc ggtattcctg caattatgtc
     6181 gctattggtc ttgatgctgt tcacggaaaa gttaatgcct ggttttgcga aagccgtggt
     6241 cagagaggca aacaataaca caatcgataa aatggatagt ctttttttca taggtcgata
     6301 acctccttgt tctttgacca tattagtggt ctttgctttt catccttagt ccatatttct
     6361 atattcgatt tttgacctat taatcagaga tcaaatcgcc cggtttgtac cagattgaat
     6421 cgttcaaata atgtatccga cgcttccagc tttcaggcat agcaaaccct ttgatgacgg
     6481 ctcccagcaa aatacccgat gttccttcta taatgatccg ctcgagagag cattccatcc
     6541 agcctacaac tccttctgga attaggacat cgatcgtctt tgcacaaaaa caagatacat
     6601 taaaatgatc gaatttatcg acatcgaatc cgctcactga gcctgcacgc tggataagag
     6661 cacgcatctc gaatgagggg aaggcaatcg caaacaccct attctcttcg atgtcgtgga
     6721 aagtcttatg tgaagtgtcg cagacaacaa gcagtttgct cacgggctca tattcgtagg
     6781 ggatgcacca ggagagtggc gcaaaatcat agcgctgagg cagatcacgg taatctcgag
     6841 ggacggaggt atgaggaagt gggctaaagc caagagaggc ggaatcttga gggagggagg
     6901 tatgagggac ggaggtttca gagtcagaga cagagccccg tgtgcaaagg nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggaggtatga gggacggagg tttcagagtc
     7081 agagacagag ccccgtgtgc aaaggaagat cacgccgcca ggattttgaa gacagtatac
     7141 ttcatttggc tgaatctcat cgtaagactg aattgattta tgagaccgat gaattgaaga
     7201 agctttcata gaaatctcct ccagaatggg tccgaacgat gctaaaggtt tctcttagaa
     7261 gactgcttta gaatagtctt ccatagactt aagccttcct attacattaa gtccctttta
     7321 tgatgtggtc aattaggatt ctgaatccga taatgattag aatgctacct cctcctattt
     7381 ctgcccattc ttggatcaac ttcctaagcc gcttgccaaa ttcgatgccg attatacaaa
     7441 cgataaaggt aatcgtaccg attatgaaac tgggaagcat gatcggcatg ccaagaagat
     7501 tataagagag ccccacagcc attgcatcga tgctcgtggc aaaagcaagt acgagcaatg
     7561 tatttagctt cataattcct tttgatgcag agtttgtgcc cttatttgta gcggcaattg
     7621 ttttgctttt tgcagcgacg actttttctg gatcatcaca agctgattct ctccgcatat
     7681 gaatagcctc atagagcatc ttgctgccta ctatgatcag cagtccaaag gctatccagt
     7741 gatcgtaatt ctggataaga gcactgaatg ctgaacctag agaccagccg acaagtggca
     7801 tcaggaattg gaagaagccg aacatgaatg ctgcgcgaat gccaatcaaa agaggcagag
     7861 cagttgcgca agccgctgca gagacggaaa ccgcaaatgc atcggccgct agagcaaaac
     7921 caaccaacac atatgtgagc ataaagagat atctattcta tagcaaagag gtgctttatg
     7981 ctatgcgttt gtctttattg cattttctag ctgttcaagg gcctttatga gcacgtttct
     8041 tggacaggct atattgatgc gcataaatcc cgagccacct gtgccaaaca tggtcccatc
     8101 atcaagccaa agccttgctt tttttaggaa tagatcgtgg agctctgcgt cgctgagtcc
     8161 caatacccga cagtctagcc atggcagata agtgccttca agcgggaaga ccttgagaga
     8221 cgggatgcgc ctgtcgataa agctctttag ggtggcgtag ttctctttga gatatgaaag
     8281 cagttcttca agccagggtt ctccctttgt gtacgcagct tcctgagcca ccatgccgaa
     8341 ggtattggtt aggttaatgc caatcttcgt ggcttgctct ccaaaagctt tgctaagctc
     8401 gcgattcgga atcacgatat tggctatctg cagacctgca agattgaagg tcttattggg
     8461 cgcggtgagg gtaatggtaa tggccgcggc cttttctgac agactggctg tgcaatgatg
     8521 aacctctgtc cccaaaataa tgtccgaatg tatttcgtca gagatgatga gaatattatg
     8581 agaggagcag atatcgatca gcttagaaag ttcatctatg gtccatactc ggcctacggg
     8641 gttgtgtgga ctgcatagaa tgagaagttt tgtctgagta tctatcttgc gatcgagatc
     8701 ttcgaaatcc atggtatagc gaccatcccg aatgaccagt gggttttcta ccagttgacg
     8761 ggaattatta agcacagcag cagaaaaagg attatataca ggcggttgta tgatgatctt
     8821 atcgccaggc tgagtataag cgagaacagc caggtttata gctggcacaa cacctggtga
     8881 aaatagtatc cagtcttttt cgatgctcca gccattgcga tgcttcatcc aatcaataaa
     8941 ggacttatag tactcttcag aacacttggt gtaaccatat atgggatgtt ccgcgcgcgc
     9001 ttggattgct tttactatat caggagaaca ggcaaaatcc atgtccgcga tccacatagg
     9061 tattgcgtct ctgcagcctt ccggcagctg gtctctatcc cacttaatgc tataggtatt
     9121 gctgcgatct actattgtat cgaaattata cattctgggg ctccttattg cttgtaagct
     9181 atcgcctcaa tttcaactag agcattttta ggaagagcgg cgacttgtat tgcactgcgt
     9241 gctgggaaat ctccatcaaa ataacctgcg tatatttcat tgacctttgg gaaattgttc
     9301 atgtctttga ggaatatggt ggtttttaca aggttggcta ccgtacaact gcaagcctcg
     9361 agtacggaga caaggtttct aagcgcttga tttgtctgtg cttcgatgcc ttcgacaaga
     9421 tcgccagtac ttggatctat gccaagctgg ccagaacaga aaacaagggc acctgcacga
     9481 atggcatggc tataaggacc gatggcagcg ggggctgtct ttgcttgaat gcattctttt
     9541 tccataaagg ctcctttaga atagggtaga agaactcata agagctccta taatttgaaa
     9601 ttgcttttcc aaataggaca ttttcaaaat ggagtaattc cgaaagaaga caatattccc
     9661 atttgtttgt ctctctgttt tgtttatcaa cgcgaaatat ggcgaattca tttgttcttg
     9721 taacaatata gaatcatttt agaagcgcat gctgggcgac ttcattcgct ttttggggtc
     9781 cttctagtac cgaaataatg gaaattgcaa attccccagc ggtgcctgga ccttgtgaag
     9841 tgataatgtt gccatcttta actactcgtt ctgcacgaaa gtgacctcct ctaatttgat
     9901 cctctgtccc cgggtaacag gtaaattccc tcccctgcaa tagattgcag gccccatgga
     9961 gcacaatagc aggggaagca cagatagcag caattagctt gcccttttca taatgcctaa
    10021 ttatcaggct tcgaaccaaa gcactatttg ctagattgct tgcgccgggc atgccaccgg
    10081 gaatcacaat tgcatcatag tccttttcca gcgcttcagg gcctgagtct gtttctatga
    10141 ttatgccgtg tgaacctctg accctctttc caacaattcc gacgacattg acatagatgc
    10201 ctgcgcggcg caaataatcg atcggcgtta cggcttcgat ttcttcgaat ccttcagcaa
    10261 gtagcatgca ggccgttttc atctttattt cctcctaaat aattgatccc aacatattaa
    10321 gctagtcagg ccaagatcaa acaaacgaga taggtagcag aagctgcgac acatccaacc
    10381 agcagcattt caaagccgct cttgaataag ttctttctat taaaattatc ttcaaagatc
    10441 cgagaatgaa cataattatt ctcgccaata tcaatataca agcaaacaga aaacggatcg
    10501 aatcgagaga aggcacaaaa agcacaaaaa cataggtcga aaaaggattt aatccagaca
    10561 aggcaagaga aatgaaagtc gcgatggcgt ttgcaaggcg tgattcatct gaaaggatga
    10621 tttcaagctc atcaatcatc atagtatcga ccaatgctgt tttacctttt gcggttatct
    10681 ctatcgcggg ttttgcatct ttttgcgaca cgcctctgct catatagatt tctttaagct
    10741 cgaatttttc tccttcagga taattctcaa cttcccattc atcgcgtgct cgtctatcag
    10801 cttcgtattc cgtttcactt ttggatgaga ggtaatagct gatcgacatt gataggccat
    10861 ctgcgagcag atctgcaagt cttatcatga gcactattcc agaagacaga gaagcacatg
    10921 caacacctga tgcagcagtg aaggttgtga tagtaccatc gaaaccgcta tatcttatgc
    10981 ttttgatatg tttatcttga gtcgttgtgt gcttttcttc ggcttggtgc ggatgagagg
    11041 gattatatgc acgacgagta gcttccgggt gcctgcgctt gtaagcttct cttatccggt
    11101 caaggtctcg tgtgagcata gaatctcctc cctatgtcat tgcatatgga tacaaacgca
    11161 atgatgaact ttggcggatc tgatgatttt ttattctagt ccaaatgcaa gcattaggta
    11221 accatgaggg acggaggttt cgataatcct tcaaaatagc gaaagacgga gattttgata
    11281 atctcggact agggtgcatc ttcgggatag atgtccttta ctacatatta ggcaggaggg
    11341 acggaggttt cgcctttcct ttgattccac gagcacaaga acttgaaatt agagatcttc
    11401 ggtagagctc tcagatagag tccttatgga gcgcttaagt catttatgat ctatgggcgg
    11461 tagacggcat ttaagacctt atgaccccaa tacgctgcga ttccttccat ttcgtcttca
    11521 atgcgcatta gttggttgta ctttgcgacg cggtcggtgc gcgacattga gccggttttt
    11581 atctggccgg tttctaggcc aacgacgaga tctgcaataa aggtgtcttc agtctcgccg
    11641 gaacgatggg agacgatagc agagtagcct gcgcgcttgg ccatgttgat ggtctctatt
    11701 gtctcggtga ctgtgccaat ctgattgagt ttgatgagaa tagagttgca ggcacccatt
    11761 tcgatgcctt tggcgaggcg cttggtattt gtgacgaaga agtcgtcgcc aacaatctgg
    11821 attttattgc ccagtgcttt ggtgaatttg acatagcctt cccagtcgtt ctgatcgaga
    11881 ccatcttcga tggatactat ggggtattta ttgatccagt ttgtgtaaag ctcaatcatc
    11941 tcatcagagg agaagagtcg atcgggactg gatttccaaa agcggtagcc tttgccgtca
    12001 ccttcctcga agagttcgct cgcagcagga tcgagggcga tggctatctg ctcaccaggc
    12061 ttatagccag ctttttcaat agcacgaatg atgtaatcaa gagcgtcttc attgccgatg
    12121 tttggagcaa atccaccctc atcaccaaca gcagtgttat gaccatcggc tttcaatatg
    12181 gattttagcg catggaagac ttcagcattc atgcggacag cttcgcggaa tgattctgca
    12241 ccaagaggca tgatcataaa ttcctgaaaa tcgatctcgt tatccgcatg cttgccaccg
    12301 tttatgatat tagacattgg aacgggtaaa aggttagtgt ggactccacc gaggtattta
    12361 tatagtggaa gacctaggta ttcagcagcg gcgcgggcag ttgccataga gacgccaagg
    12421 atagcgttgg cgccgagcct gcctttgttt tcggtaccat cgagctctat gagagtgcgg
    12481 tctatatcga cctgttcaag agaatcgagg ccgcaaattt cgcctgctat gatagtgttg
    12541 acgttttcaa tggcagcaag aacacctttg ccctggtagc gtttcttgtc gccatcgcgg
    12601 agttccacag cttcatattc cccggtcgaa gccccagatg gtacagcggc gcgacctaga
    12661 gtaccgtcct cgagcacgac atccacttca acagtcgggt ttccacgtga gtcaagtatc
    12721 tcacgagctt cgatatattc tataatgctc ataaagattc tccttttgaa tatatatgtt
    12781 ctgaacgata tcgatattag tttgattcgt caagacttgc aaggtttcgg aagatttcga
    12841 gtacgggtct ttggtttcta ttgcaaagac aaattgagtc aactgaaatg gtagatcata
    12901 tcttgccagg aattgctgat acgcgaacat ctgcaggcat atcatcgaaa ttccacggga
    12961 gggtaaccgg atcgatgagt ttttgagaaa acgctttctc aatgaaccaa tgcagaagcg
    13021 caagtgcttt atttgaggcc gattcaaaga ctcgatctcg tgcaatcgac ggcgattgaa
    13081 ctgaagtgac aaaatagaga agaataccat ttgtaccatt tggggacgga gctgcaaaaa
    13141 atcctgtgcg cagtccattt attggcagga cgggaatgcc gatcatgcgc acaggagtca
    13201 cattcttgtt agtcattaga aaataagaat tcgtaataga acaatgcgct tctaatatta
    13261 atgcgtcgaa tgtctgatct ttctggtgga gatacatagg ttttgaaaga agcgctttca
    13321 attcggatgc tgttgatgga tcgctgattc tgtttctgct cttaaggtta tcgatgcggt
    13381 acgattctac atagagagtt cgcattattt ttctgcttgc cgaccaatac tgaatgcctt
    13441 gtatagaaga aaactggttg aatattagcc caagcgaatc aagttgagca gagattgaag
    13501 atggaccttc aacagagctg ttgggcagga agatcagagc ttcaatgata agattgctct
    13561 cttttgcagc agctttaatg tgctcaatta taaacgagac accatcgagg gacggagcta
    13621 acaaaagctc gcctccaata tgttcagacg tgcgaaggat gggagagcct ggttgaaaac
    13681 taatgcactt gggcaaaact gcattaaaag atggtggtag aggggagttt attctagaga
    13741 tgtcctttat accttgaaag cttgacgacg atggcggttg ttgagctgga agattcgcaa
    13801 gaaggattag cagaaaaatg gtgatcaaaa caaaaaagcg gataacagct tgagaagctc
    13861 tcaacctccg tccctcagtt agtatctcag ctttcaacct ccgtccctca gnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcaacctcc gtccctcaga gactccattg
    14041 tctctattgg ttttaagttg gtggggaaaa tattgattat ccaagaaagc tctattcaat
    14101 cttaagaact tcatagattc gctcaagatc ctcttgggag aagtagtgaa tttttatggt
    14161 accggaatca atgctacctg atatctcgac ttttgtaccc aatctttcga tgagtttttc
    14221 catgagctct tttaaaagag ggtcgagagg aatttcctca attgtcttaa gagattcaag
    14281 ctgcttttta gattttgtcc gctggcctcg gagcgcattt tggatttttt gagcttcttg
    14341 ttccgcttgg cgaaccgata aattgttctg ttggatgtgt tcaaagagct ttctacgttc
    14401 tttttcttct tctatggaaa gaagagctct ggcgtgtcca gcgctgataa taccttgttt
    14461 gagcagaagt tgcatgtctt ctggaagctt aagcaggcga agagtatttg cgacagcgac
    14521 gcggcttttg cccaccattt cggcgacgcg ttcctgagta actcctgtta tttccatcag
    14581 ctttgcataa gcttgagctt cttcgatggg atcaaggtct tcacgctgaa tgttttctac
    14641 cagagacagc tctatgcgat ggttttcgtc atcctgactc cgtactatga cgggaagttg
    14701 ctccagccct gcaatctgcg cagcgcgata tcttcgttct ccagcaataa tgatgtattt
    14761 ccctgatttt tcttgatgaa cgagaagcgg ctgcaagagt ccatgacgcc gaatagattc
    14821 agcaagctca tctaaggatt cctgagtgaa ggattttctt ggttgatctg gattgggaat
    14881 gaggtcttct atcgatacaa gcttcacaat agatgcggat gtttcttcaa gagattgttc
    14941 gaaaaattgc tgatgttcgg gaattaaggc gccaagtccc tttccaagac cgaatttagc
    15001 cacggtttat gatctccttt gtgacagctt catagctctt ggctccaatg caggtgggat
    15061 catacataat ggcagggagc ccatgcgatg gagcttctga gagacggaca tttctcggga
    15121 tgattgtctt gaagactttg tccttgaaat attcggttac ttgcctgaca acttcttgag
    15181 aaagtcgcgt gcgagcatca aacatagtga gcaggatgcc attgattctt aaagaaggat
    15241 tgatgctctt ttgtacaagc ccgatcgtct ggataatcag cgagagtccc tctaatgcga
    15301 aatattcgca ctgcaagggt attatgactt ctgtagcggc tgccagtcca ttcaacgtaa
    15361 gcactccaag ggacggagga cagtctataa ggatatattc ataatcattc accacagggg
    15421 caattgcgcg tcttagatag tcattccggt cgggacgatc gacgagctcg atagttgcac
    15481 cactgaggtc aaccgaggag ggagctaaga aaaggcgcgg aacagggact gactggatga
    15541 tttgttgtat ctctgcaaga cctgaaatca catgatatat attggtgtgg gataacttac
    15601 ctccaattcc tgatgtgagg tttccctggg gatcgaagtc tatgaggagg gtttttttat
    15661 attgcagggc gagggatgta ccaatattaa tagcactcgt ggttttccct acacctccct
    15721 tttggttgac aaatacgata atacgagcca ttctgccttc ccgatatgct aaaatgattc
    15781 taacatattg atcgagcttc agctatacag gtttagcgca taaaaagcag ttaatacaat
    15841 tacatacata tcgaaaataa tgccaaaata gttttgtcta ttggtattca attatgttgc
    15901 tatcttgagt gtctataaaa gactgaaaag tctaagcttg taagagtcta gccagccttc
    15961 ttgacctatc ttggtcaaat ttgataattt aatcttaata aaggagatat gaatgctgtt
    16021 cgaacaggtt aaatccgcga tagaaaatgt acgaccatct ctcaaagcag atggaggaga
    16081 tatagaactt gtatctgtaa gtgatgatgg tgtagtcaaa gtgagactca ccggagcatg
    16141 cggtgcatgc ccttattcca tgatgactct caagcaaggc attgaagcgt acctcaagaa
    16201 agcagtgcca caggtaaaag aagtcaaaca agcctagaaa attctagttg ctggaaagaa
    16261 ttgcactccg gcttaatttc tatttgttct agattggatt tactattttc gcaaagcctg
    16321 gtcttttgct aattttcaga gattttcata aaatcccatt gttcttgata tttcattagc
    16381 tgccctgact acaaataagg cgactttttc cgcctccaat tctggtcgaa tttccaaaag
    16441 ccatgatgtg ctgatagaag cgatgatttg accacgatag tctcgaattg gagccgccac
    16501 gcaatttact ccaggctctg cctcctcatt gtcgtgagtc caccctctgg ccttacaaat
    16561 atgaatatct tttatgagag attccacatc aatatgcgtg ttgggaccaa atttcgttaa
    16621 aaaagtatcg gggataagat tacgaatttc atcatcatcc atatctaata tgagagcctt
    16681 acccagagat gtgcaataga taggcctttg ttcgcctatg attgcatatt ttctcaggct
    16741 agtgaactga tcttctttat caatatatac catcatattc ccctgacgac gtgcaaggaa
    16801 tacgatcgtt cctaacgacg atgctagttc ccgcatgaag ggagcagatt ctgtcttgag
    16861 ttctagacta ttcagatagt tgctagaaag ctcgataaaa cctggtccca ggcgataaaa
    16921 gttatcgtat gggccttttc gaacataatc gcgctggaga agaacagaaa gcaaacgaaa
    16981 agtcgtactg cgaggaagat ctgactctgc agcgatctct acaagggtaa gaccagttgg
    17041 ctcccgtgca aggatttcga gtatatcgaa ggcgcgatcg agagactgta ctttcatatc
    17101 agccataagg cctccttatt cattaatcta accataaaga tatattttag tattgacaag
    17161 atagaaaagc aagctattat atggaattgg attccatatc gtggaatcaa tttacaggac
    17221 gtttttgggc gcaatgctaa acatagccat taaacctaaa aatcatgttc cattatttat
    17281 gatgcaagga gcaaagatgg aaattcgaga agcgattaat tctgagtggg caaaaaaaat
    17341 ggggacagag gatttgcgca atgcgtttct ggtggagaag gtttttattg ttggagagat
    17401 gacagccatc tatagccatg tagataggat gataattgga ggcattgtcc caacctccgt
    17461 ccctcttgcg ttgccagtga gcaaggaatt ggggacagag ttttttttag agcgacgaga
    17521 gataggaatt ataaatatcg ggggagaggg gtttgtcgag gtagatggta ctatccatga
    17581 tattggacaa cgggatgcaa tatatgtctc gatgggttca aaaaatgtag cattttcaag
    17641 caagaatcct aaggagccgg cgaaattcta tttcaatagt gcacctgctc atagagcatg
    17701 caaaactagg cttatcactc ttgatcaagc caaccatgtg gcgttgggta gtgatgagga
    17761 gtgcaacaaa agagtgatca atcaatatat tcatccaaca gtccttgaaa catgtcagct
    17821 tgtaatgggc atgacgactt tttcagaagg gagtgtctgg aacacaatgc cagtgcatac
    17881 acacgagagg cggatggagg tatatctgta ttttgatatg ccagatgata aagtagtatt
    17941 ccatttgatg ggcaagcctc aggaaacgcg tcatattgtg gtacgaaacg agcaagccgt
    18001 aataagtccg agctggtcca ttcatgcagg agtaggaact ggcaaatata cgtttatttg
    18061 gggcatggct ggagaaaacc agacatttga tgatatggat aatgtggaaa tggctgatct
    18121 acgttaaaca gatctgtttt aaaaggagaa aagacatgat tttggatgaa tttaggctcg
    18181 atggcagagt ggcgattgtc acaggtagtt cgactggtct cggacaggga atgagcatcg
    18241 cgctagcaga agctggagcc gatgttgtgg gagtagatta tgtgccatca aaagaaacgg
    18301 ctagcatcat agaggcgcaa gggtgtaagt acttggagat cgtggctaac cttctgacaa
    18361 tagaaccaat tcagagcatc ttagataaga ctataagtgc ttttggacga gtggatattc
    18421 ttatcaataa tgctgggact attaggcgag aggatgcaat taagtttaat gagaaggatt
    18481 gggacgaggt catggacctg aatgtaaaga cagtattttt cttcagccag gcagttgcgc
    18541 ggaaatatat cgaacaaggg acgggaggaa agatcatcaa catcgcatcc atgctttcat
    18601 ttcaaggagg tataagagtt ccgtcttata cagcttcgaa aagtgctgtg atgggtctta
    18661 caaggttatt ggccaacgaa tgggctaaat atcatatcaa tgtaaacgca attgctccag
    18721 gctatatggc aactaacaat acggcagaac tgagagcgga tagagagcga tcagcggaga
    18781 tcctaagtcg tataccagcg ggtcgatggg gaacgcctga ggatgtcaaa ggcgccgcag
    18841 tcttcttagc atcaccagca agcaactatg tgaatgggta tactattgct gtcgatggtg
    18901 gctggctagc tagatgactg tcataaaaaa gatatggttc cttgtgtaga atttatggct
    18961 ttagtggcag gagggacgga ggtttgttga ttattgataa gagcgattat ttattaattc
    19021 ggaaatgcct atataaggag taaaaagatg attgtttgca tgctttcaga tctatcaaga
    19081 tatcgaggac tcagtaagaa tctagatgtc gcgtttgatt ggctccataa ggggctttgg
    19141 cgtcagcttt caataggaaa gcattctgtt gatggcgatg ctgtctttgt gctcattcaa
    19201 gaatatacag ctaaggagca ttcagcgtgc agatttgaat ctcatcgaga atatatagac
    19261 attcaaatga tgatttcagg aaaagaaatt attgaggtgt tgccagcaaa tgaactcaat
    19321 gtcatcgagc cttacaagcc tgatatagag ttttatgcct caccaacaga tagaaatgct
    19381 aatgttctgg cgatgagtgc tgagcaactt gcaatttttt ttcctgaaga tgctcatcgg
    19441 ccatgcatga aagctggaac attggcggaa cctgtgaaaa aagttgtggt taaagtggct
    19501 atataatata agccttaagg ctagaagaag gttcgcttta ctgattttga tcagaaagag
    19561 aagagaggta agagggcaaa tcttatctag ctggaatttc gattgcgaat tgcgtactga
    19621 aaaagtctgg aaaattggga atgcatgaaa tcggggagga aatcggggac agaggatttc
    19681 gtcacagaac agttaggact gaaatattct gtcgatttga tattttgtcg gctctagcta
    19741 aaggcgagac ttgactttgg ctatgtggac tagtacgtaa catttttgag gattatgcaa
    19801 tggtaaaaaa agagaaaata ggagaactgt tttataaaac tggcctcatc cctgtgataa
    19861 agattgattc tgaggagatg acagatggtc tagctaaagc acttcgcgga ggaggattgt
    19921 ctgttgccga gatcactttt agaacaaagg ctgcaccaaa agtaatcgag agatttgcat
    19981 cgtgccatga ggatattatt gtaggtgctg gaacagtgac gaagaaagat gaagtagatg
    20041 cggctctttt ggcaggagcg cagtttattg tttcgccagg ttttaatcca tctatatgtg
    20101 aatactgctt gggaaaggac gttgctgttt tccccggtgt aaacaaccct tcgcttatag
    20161 aacaagctat ggctatgggt cttagtatct tgaaattctt tcctgcagag gtttcgggtg
    20221 gagttaaggc tcttaatgca ttcaaatcag tatatcagca aatatcattc attccaacag
    20281 ggggaattca ggaaaacaac attcatgaat acctttcttt gaagaatgta ttggcatgtg
    20341 gtggttcatg gattgtttca gccgatcttc ttgaagctcg taaatttgat gaaattgaaa
    20401 gacttgtaaa atctgcacga cgagcgatga tgggattctt gccaatcgtg caggacggca
    20461 taatagatga agaaaaccag attagatcaa tgagggacgg aggttgtatt ggaggctgta
    20521 ttgaagtcaa aaccccatcg atcaaacgaa cattagctat gctttcctat ttgaaagcct
    20581 atgttgtgcc tggttctgag gtcgaaaaag gacaaggttt ggtggaggtg agtataagta
    20641 ttcctggatt gcatacccag attcatcttg tagaataggg aggagaatta gaatgagcat
    20701 aacgacattt ggggagatta tgcttcggtt aaaatctccc ggacaggaaa gattgcttca
    20761 atcgcctttt tttgaagcga cttttggtgg aagcgaagca aatgtggctg ttgctctgtc
    20821 aagattgggc attacggcgc gatttattag cattttgcca gacaatccga ttggcagagg
    20881 atgcatgaga gagcttcggt ctcatggtgt tgatgtttca tgcctaaaat tcaaaaaggg
    20941 aaggctggga atatatttct ttgagaatgg agccaatcag cgtccaagta atgtcattta
    21001 tgatagagaa aactcagcga tttccagaat tgctgaagag gatatcgact gggaagcttc
    21061 ttttaaagat atgcaatggt ttcatgtatc gggaattaca cctgctcttt ctcagaattg
    21121 cgctgatgaa acgataaaag ctgcttgcat tgctaaagac aggggattaa ccgtatcttt
    21181 tgatttgaat tatagagcca aattatggaa ttatggagta gatgcatata gtgtgatgtg
    21241 taaaattgcc caatatgcgg atatccttat agggaatgaa gaagattatc aaaaatcatt
    21301 agggattaaa ggtccttctg caattggagc tcaacaactt gatattgatg cgtatcaaga
    21361 gatgtgcgag actgctttaa aacagtatcc gaatgcaaag atagcagcag tgactcttcg
    21421 agaaagttac tcagccgata ataatgattg gtcagcgatg attgtaagtg agggacggag
    21481 gtttatctct cgcaaatacc atatatcgga tattgtggat agagttgggg ctggagatag
    21541 ttttgtcgcc ggcttaatat acggattaat cactaagaag gattatcaag cagcccttga
    21601 atatgcaact gccctttcgt gcctaaagca ttctatttca ggcgattttg cccttcttga
    21661 aaagggagag gttgagaaac tcttaggagg agatgcttcc ggtagagtgc aacgttaatt
    21721 cgctttctca aatatgctct ggctttgatt aataaaagga tgagggtaag tctcatggct
    21781 tattagctgc gccataagga acaacatctt cattcgcatt ctcatccttt ggctgatttg
    21841 gagaggttct actgctgctc gtactctcca actttctctt aatgaggcgt gctaccacat
    21901 cacgattatc caacagagag cttaaggatc tatcgtgatg cagtaagcta atcacctgtg
    21961 caaaaaggcc agtgacatcg ctctggatat accattcttt ctttagcaac tcattatgat
    22021 atatggcatt tgtgcctatt actctataga ataacccttc tttataagca gcatcaaagt
    22081 catcaataga atttgctgaa aaaattggaa gactaacagc acatataacc ttgctagcgc
    22141 caagttcttt cagatatctc attgccttaa taagagttcc accagttcca atcatatcat
    22201 ctgccataaa gacaacttta ttcgagacat tgccgagcaa tcgcatttcg ctgatatttg
    22261 tctctgttgc gcttttagtg atttttgaat agtctctttc tttgtaaagt aaagccaagg
    22321 gcttttgcaa agcatttgca tagaatttat ttcgatcgac cgcaccggta tcgggtgcga
    22381 gcaccactat gtcttctttt gctatatcga taatcgatgt aagtttttca attatctgaa
    22441 aagaggcatg taggttttcc attctgagcc ggtcaaatgc attctcaatt tcctttgagt
    22501 gaatatcaag agtgatgatc ctattaacac caaggctttc cagcatcttg ccgattcgag
    22561 ctgcagtcaa tccctctctt ccttttttct tatgttgtct cgaataggga tatattggta
    22621 gcacaagatt tatttctgca gctccagctt gcatggcagc atcaatagct acaaaaaggt
    22681 taaaaatgtg atcatttaca gaaaggactt tttggatagt accatcatta aataagacga
    22741 gttgatgatt ttccacatcc tgaaaaatat agatgcttct tccccggata gattcaagga
    22801 tttctgtttt tatttcacca tttgcaaagt aagtatgtct tacattaatc ttgaattttg
    22861 gaggtcgata acgttcttta tctcctggca agaaaagcat gcctgtgtta cagtcatttg
    22921 caaagttgat ctttctaata accaagtcgg gcgtgatatg atatcttcca gctatagcct
    22981 gagcttttct catgaatcga tgcttataga ttcctgagag ctttcggatg acctgatttg
    23041 cgaacgcttc tgctcctggg caagcaataa tgcctaaatg ggttggatct gaataactca
    23101 tgcaaggcct tccgtatttg aattcaataa actgacgcgg atgagtaaca agagagatcg
    23161 ttgctattat agaccataat cttccaaatg aatagaatct cttagatgag aattgtagtg
    23221 ggtcatccca agggagcaaa ttcatttgct ctgtatgcgg agcactatgc aggatccaag
    23281 atcacgaaaa gcgtcgatgc tggcatttga gaatctgtgg atttgtgcca attcttgaag
    23341 cgctatactg cgtccctttg tataaagcct tttgttctgc tttttaatgt gaatcttatg
    23401 cacctcatgc catgtgtttc tttattcgga tgcctttgta cttgagtgcc ccacagaata
    23461 tttggatttt gaaacgctcc gcgcaggaat ctggcttgag acaggtcaac agccatcgct
    23521 aaatgcgcac aaccatgatc gattttcgca gtggtcgtat gtgcgacctt aatacgattc
    23581 ggtaggccgc cttttgcctt atatcgcaag agatagtggc atttctttat gcttgtcgcc
    23641 ggttttgaaa actgttcccc tgtataaatg gctcgcaaaa tcgctctttg cataatcctt
    23701 tgcgcatgct tccgtgtcat ataaagattt gtaagttcat acgcaatatt gagatatcgg
    23761 aagatagatt tatcgcgtgg aatcacaagg atttccatga gcatttagag gtcgtcattg
    23821 ttttttcgta tgagactagc tccccagcaa taggacattc gtgatatcct taaaaggtca
    23881 aattggcgac gcaagcaaag cctcagatct tgctatacca ttttggtttg gtgcggtcca
    23941 gttgttagta ttctttgaag aattcgttct caacttgtaa ttgtccgttt tgcttggaca
    24001 gttctgtttt gattatggtt ttctatgttt ctttgttctt tgtgtctttc tagaagagcg
    24061 gagccctttt ccccgaaagc tgtttttttc caaaaggcga ctagattggt atgcatttgg
    24121 gtttggatgt acctgcaaaa tcatgccgat ttcctgaata gtctttttgc cttggttact
    24181 tcaaccgccg attttgcgtt gattcctttt tgtactactt gagcataata gacttcatgc
    24241 ctgcttgtcg ctatttatac aaaaaatatt gaattacttt attatcctgc tgatttgttt
    24301 tagtcttgag ggcttactat gttattttga ttataactcg aaatctttct ctataatccg
    24361 aaaatcattt tttgaaatga agtatgcacc gcaatatcga tattctgagg ataatgtgca
    24421 aaggcccgct tttatagggt tattgcttat atattcatat accaaaataa attgccttat
    24481 ggtgttaatt agcacgctct tgaaacggtc ataccataca tgacctttag attgtattat
    24541 tttattataa tgtatagcaa atacggataa tatccattgc attattttgg atagagattc
    24601 tcctcttgct ggtctaatta aaaaatgaat gtggtttccc attatacaaa aatttgcaat
    24661 ttcaaattta taaggatgtc ttgctttagt ttttgcacgc agaactacct tgagaaaaag
    24721 gtcctttatc cctttattgt caaatataca ttctgctcta ttgatcttgg catatacatg
    24781 gtaccaagat ccttctgata atatccttga atgtctcata ttaaaaacat cgcagaaaga
    24841 agaaggaata attcaaaatt gatgatctat cacttgaaaa ttctcaataa cccgtttcca
    24901 acttgtaagt agcacagaga gagcagtaga tttgagtacg atttagtaat atctagctac
    24961 tattaaatca ttgcagatta ataaattagg tacgggctag tgctgcatgc caaatatagg
    25021 ataaaatata agataaacct ccgtccctct attgctnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25141 nnnnnnnnnn nnnnnntaaa tttgtattat gtcgagggat gtcatcatat gtctcatcaa
    25201 ttcaagtgaa aatactgaac caagctttca aatttatgat aaagtagcat ctatatgatt
    25261 cacagaaaca gtaacataga tactgaatct ttagcgattc gattggccag attttctagt
    25321 ataactagaa cctcaggaga attatcttat cctgattttc ttatttctgt tcttcgagaa
    25381 atttccttct ttagtaatag gccaaacctt gttcaagaat ttgagatttc tgaggataaa
    25441 tggaagcgga gaattgtaat tgcttttgct cccggagaag ggcaagcatg tataacgctt
    25501 acaggccact atgatgtggt tgatacaagt aattatggag ccttagagcc tcttgctttt
    25561 gatccagagg cgcttttgaa aaaaatgctc caagtgcttt ctgctaaaga gggcctttcc
    25621 tcttcagaaa tatctctcaa aaaggatttg gaaagcggct atttcattcc gggcagaggc
    25681 atcctcgata tgaaaagtgg actagctgct gcaattactg tgatttctag gttcttggaa
    25741 gatcctgata ggattgggaa tatcattttc ttagcagtgc cagatgaaga gggggcttct
    25801 gttggtatga aagcagctaa gaagattctc tcagatttta ttatttctca tcagcttcag
    25861 atgagagcaa taattaattt ggacgctgct gtcgatcaag gggacggagg tgaagccaaa
    25921 gccgtatttc ttggatcagt tagcaagctt cttccatttg ttttgtttgt agggaaagca
    25981 gctcatgcag gtgcaccttt tgatggcttt aacccggcgt tagctgcttc tctttttata
    26041 agagagatag aatgcaatag cgattgcttt agccctcgtg ctatatctcc tggagaggaa
    26101 ccgcctccgc caaccattct ctactgccga gacatgcgga cacactatga tgtaactatg
    26161 ccttttgata tattttgtgc tgttaatatt ctgacctttg aaaagaaacc tgaaaaaata
    26221 ttcgaaagtt ttaggctact tgtggaggag atgctgaata atgctatttc cctcttccat
    26281 gaacggaagt ctgttttttc cagaaaaaag aatgagcatg ttgcaatgca gagattttct
    26341 tcgagggtta ttgatttttc ggagttggtt gatcgtgctg aatatgttgc accgggtatt
    26401 ctggatcgac taagaaaagc agccgaaaag tatcatcccg aggatcccgt gttacaaaca
    26461 gctagaatag tgcatgaatt gctttcattt gcccaaatcg aaggaccctg cgcaattatt
    26521 ggctttgctc ctccttttta tgcaaaagcc gaattagata tatatagaga ccaagagttt
    26581 ttggttctgc tcaaagacga gattgctcag ttcaatagag aatatcaaga aacaataaag
    26641 ctccgtccct tttttcctgg aatttcggat atgagttttc tagcaccatc aatagagagt
    26701 catgcgatag aatacatacg aaagcaatca gcggtaattc agaaagattt ggacgagtat
    26761 atggatgatt tgatagaagc acctgtaatt aatattggtc catggggtag agaatatcat
    26821 caatggggag aaagggtaaa tcgccactat gctttcaagg tcttgccgga tttagtattc
    26881 agaatcagcc agaggatatt gagatagctc aaataattca ttatttaatt ataccttata
    26941 tgaatataga gaagacatca tataaacaaa agggggaata atgatgacta ttgtgatatc
    27001 tttaggaggt tccatagttg ctccacaaga gggacctagc gggatattct tatatgattt
    27061 tcgaaatgta ttactttcct ggataaatgg gggggatcat aaggcgatca ttgtggttgg
    27121 tggaggttat gcagctcgtc agtggcaaaa ggcttataag gaaatgcttg aaatggacaa
    27181 agcgattagc agggacggag gatcaagcat aagcagaaat atcgatgttc gccaatctct
    27241 tgatagaata ggcattgcag caacaaggct caatgcacag ttgattaggg aggttttttc
    27301 tgattattgt agagatccaa tagtcacaga tccttccgct gatattacaa tgaacggcaa
    27361 aatattgatt gccgctggct ggaaaccggg gttttctacg gactatgacg cagttattct
    27421 tgctgagcgt tttaatgcag aaaagatatt gaatctctct aatgtccctc agatttatag
    27481 tgccgatcca aaggtcgacc ctaatgcaaa gccgcttttt catatatctt ttgattctct
    27541 tttgaagatg attggaaccg aatgggatcc tggagcaaat gtgcccttcg atccaattgc
    27601 ggctgacaaa gctcgcgaac tcggtcttac ggtgatattt gcatcgggga ccaacttgat
    27661 gaatttcgct gatattttaa atgatagagc atttataggc acaataatcg attaaattta
    27721 ttctgttcta aattgatcgg cttaaggcgc cagatcgaag ggacattcga acttggcaat
    27781 ttcctgtatg gctcacatgt gttttaacat ttcagattgt tcttgttctt gactccaaga
    27841 tctgaagttg gcgcaagact tgattttcta tatcttgtcg atgagcatag aacattttcc
    27901 ggaaggtata gggagcgtct tcttcttttt gcctaataca ttgatggtga aataatatag
    27961 cttctcgctc tccatatgaa tttcccatcc tcttcctgat ttgctagaaa gaatggttat
    28021 catgttgcgc ttaagagaaa ggttctcaat acccataact tcaagatttg ggattatcca
    28081 gtcgaccgtt tttcggggca gcgatatata tagcccaata atattctcaa ttatcaaggt
    28141 aacggtgctg aggccattgg aggggaagtg atttggcaga ggccaatccg gatgaccttc
    28201 ccaaatggct tttccttcat tcataggctg ataggcgctc caaacagtag gatggttgct
    28261 gcgcttgtta gcttcttgat cgctgttgtg catggaatct agaatataat agagatgcct
    28321 aattgccgag tttctggcaa gttcataaaa atagtatttt tccaacccct tgatgaccat
    28381 aaatgtaagc caaggaaaaa cgcttcctcg gaatccctgc ccctttttat caaaggctgg
    28441 atgatcgact gaaagcgaag ggaaggggtt gtcagtacca aaacaggagg gatcttttaa
    28501 tttggctata agtttttccg cacgctcttc attaggaatt tcggctaata gcagccaata
    28561 tgtccctagc gtttttgtac gtacttggat ctcgttttca tccagatcat aatagaagcc
    28621 gtcttccggg ttccacattt tcgaatttat tcttgtcttt atggaaaaat agtctctttt
    28681 atattgaaaa gaagcgtcct tatcattaag tatgtccccc agagcagaca aatatagggc
    28741 attcatagcc atggttgcgt tgaaatcaat aaaatatcga gcctctgaac gtggagaatt
    28801 tatcatgcct gtagaagaaa gaggtgtaga aaaaagcccg tttggcattt tgaaatttgc
    28861 ctcaagccac ctgtagtaat tgctcagtat tggcattatt tcttttactc tttttttatt
    28921 ggctgtctta tgatagagat tgaactctgc ccatgagaga aggggaagtg ttacgccaag
    28981 agggttatct tccgggaaaa taggagcgcc tgtctctata tcataggcgc tgcgaattgc
    29041 gccatctggc tcttgaagcg tataaagggc atccagtcca tttgatgccg gataaatcct
    29101 attgctatat actagaaaga aactggacaa gatctgctcc agaaaatcaa gagtcttcct
    29161 tgcaggataa tagaagaatc tgatcttgct gattttggtc tggctacctc catttgtcca
    29221 gcaatcagca atccatgcc
//
LOCUS       DAOOWY010000015        28259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1677_length_28259_cov_10.000035, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000015 DAOOWY010000000
VERSION     DAOOWY010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 28259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28259
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1677_length_28259_cov_10.000035"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2695
                     /gene="dnaE"
                     /locus_tag="PK105_02395"
     CDS             <1..2695
                     /gene="dnaE"
                     /locus_tag="PK105_02395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU60588.1"
                     /translation="DQFYLKSAEEMASIFAEVPEAIANTLVINEMAKLKMEFPGPLLP
                     NYQVPEEFSSPEEYLRHIARQGFQSRYPNPNAEAIHRLEYELDIIIKMGFTGYFLIVW
                     DFISWAKEHGIPVGPGRGSGAGSIVAYAMRITDIDPLKYDLLFERFLNPERVSMPDFD
                     VDFDFERRGEVIDYVTHKYGSDRVAQIITFGTLKAKAVIKDVARALDIPFDEANQIAK
                     LVPEDPKMNLQKAIKKEPKLATLAQNPSYFELFSIAAKLENLHRHSSIHAAGIVIGKT
                     DLIDYVPLYKDPKTGLIATQYTMEYLEACGLVKMDFLGLKTLTLIRNTMELLRKRGIY
                     LKEEDIPEDDPRTFKMLGEGKSTSIFQFESQGMQSILKRAKPSSIEDLIALNALYRPG
                     PMEFIDQYIDSKNGKIAISYPHPSLEKYLKGTYGVIVYQEQVMQVAREVAGYSLGRAD
                     LLRRAMGKKKPDILQREEAPFIEGALTRGYSREEAKRIFDILTPFAGYGFNKSHAAAY
                     SVIAYRTAYLKANYSVEFMAANLTNEISNTDKLTVYISEARQMGLSVLPPDINRSEAK
                     FTVDNGQIIYGFLGIKGVGEGIAMEIQKERESHGRFIDFLDFLTRMNNAGINRKTMES
                     LIMAGCFDSLKKGRHELMGNFERAMEYVHEKSSFEASRSGMLFQEDDLGIYPDFVFQP
                     FEEFSNQEMLQAEKELLGFFFSAHPMDEYRIIWERSSTLDLGHLDQASTNREYIVVAL
                     LKEFRIYTTQNGRRMAFGKLEDYNGSIEMVVFPDLFEKNEASFVKDRVLFLKGVLDDS
                     RRDFSLKVQAILDAASLKNNSWRELHIRFKRAAIEEADLYPLRDALYSIHGQCRVIFH
                     VPMKGGAETLIEAGAHTKCSARDEDMAFIERQAAVAEVWRK"
     gene            2692..3528
                     /locus_tag="PK105_02400"
     CDS             2692..3528
                     /locus_tag="PK105_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU60589.1"
                     /translation="MKIKILGNGGFYNEGLAYNALAIDGHVLVETPPDILQSLHSQGM
                     KPSQIDTVYISHNHGDHCFGFPFFFFNWLYAGGSDSHYEKQTELVIIGPEGLKEHLKA
                     LMRLAILPEHQYLFDFDQRVKFVEIDERSIVSTSGGLWFGFLRTKHFVPTFSLVVGKT
                     GPDKGIPSCNDYLENAIFIYSSDTSMFDGIYKLLSSSARLILCDTNGEEDNGVHMSPR
                     ELMAAENQVRTRSSERADLSEYRLSGENDKDRVLGIHLSKKMDRAQGLKFAQPGEEYC
                     IE"
     gene            complement(3556..4242)
                     /locus_tag="PK105_02405"
     CDS             complement(3556..4242)
                     /locus_tag="PK105_02405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU60590.1"
                     /translation="MEGRYRLRAVAFDVDGTLYPEYSIALRCIDLFLSHPRIFMAFSE
                     VRKEIRFLQAQPNYIPRNMEELHSFQISLVAERLRKDELQIGQSIERIFYHLVPERFS
                     LIRPYKGTRQAIEAIRQRGITITALSDMPPQNKLKSLGLDDLFDFKLCTEDWGVLKPH
                     QRAFDGIIQTIRIQPSEILYVGNNVEYDILGAKKAGMRAALRGRQNSDADLSFSNWEE
                     LTEWVVKHID"
     gene            complement(4353..6122)
                     /locus_tag="PK105_02410"
     CDS             complement(4353..6122)
                     /locus_tag="PK105_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOU60591.1"
                     /translation="MENLIDITIDGQTIKVDSNENIVEACARAGVKIPTLCFLKDISE
                     NASCGVCVVEVEGAKSLLRSCVQKPTPGMKIRTSSPRVIQARKAAVELLLANHPDECL
                     SCLRADTCELHTMANLLGVHADRFPGYKKYPPPDNSSEGIVRDDRKCILCGRCVSVCE
                     DTQGVSAIAFTGRGTRTRVATFMDRGLAQSACVQCGQCSLVCPTGAITEKDESQDVYG
                     ALGDSRLSVVVQTAPAIRASLGEALGLPAGSLVTGQMVAALRRLGFSRVFDTQFAADL
                     TIIEEGNELLERLSHGGTLPMITSCSPGWINFIEGFYPDLLNHVSTCKSPQQMFGAVA
                     KTYYAQNANIAPKNMRVVSIMPCTAKKYEARRKEMDSAWDWWKEKDPETTGLRPFFDV
                     DWALTTRELARMIRLAGIDITALPQEDFDDPLGKSTGAATIFGTTGGVMEAALRTVYE
                     IVEKKPLQNMDFVQVRGFDSIKSAEVVLGGSPVRVAVAHGLSNARILLDEIRAGKSPY
                     HFIEIMSCPGGCVGGGGQPVLADIEKKRARSLSLYEEDRRLPIRKSHENPAIVTLYKE
                     FLGKPLGHLSHELLHTSYKARVF"
     gene            complement(6136..9330)
                     /locus_tag="PK105_02415"
     CDS             complement(6136..9330)
                     /locus_tag="PK105_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU60592.1"
                     /translation="MNKNTDLGFLAQEGFSHFHGDTPWISVGMGTCGIGSGADKVWET
                     LSQKTENTNIRLRRVGCFGFCAAEPVVMTWRPGKPVLFFTEVNTSKASRIFAGLSSDL
                     GYEKIAKMAEAKIEEWDFRTSKVDFGEGYDFLPTWKDINFFRDQEKIILRDAGMIDPE
                     CIEEYIAVGGYGALVRALTSMPPEAIIEEVKKSGLRGRGGAGFPTWKKWSLMRKSLID
                     NPGEAYLICNADEGDPGAYMNRNEIESDPHMLLEGMLLGAYAMNVSKGIVYVRAEYPL
                     AVYRLNLAIETARQHGLLGKNILGSKFSFDIDLVTGAGAFVCGEETALIASIEGNAGR
                     PRPRPPFPVEKGLYGRPTSINNVETWCNIPVITARGGEWFAGFGTPMSTGTKVFSFVG
                     KVRNTGLVELPLGSTLESVIYGICGGMGPKRKIKAVQCGGPSGGCVPASLFKTPIDYE
                     HLNELGAIMGSGGMVVMDQDNCMVDVARYFTGFVSGESCGKCTSCREGTSQMLHILEK
                     VSDGEASEEDLDTLEELAIIVKDSSLCGLGQTSANPVLTTLRYFRDEYIEHIKGKRCP
                     AGVCENLYIALCESSCPLHMNIPGYLQLLKENRIEDAFELTLRENPLPGTIGRICHFH
                     CRMRCRRDMLDEPVSQGEIHRYLADTMYKMGREKQVYTKLLKEKLPPSGKKIAIIGAG
                     PAGLTAAFYLVRLGHEVTIYESRQEAGGVLRWGIPSYRLPKDILKKEINLIEKLGTRF
                     VFNTRITEPEQWKKLYEENDAIIVAAGASSELTMNIPGEDAIGVYKACDFLNALAKKE
                     KVHTGGDVVVIGGGNSAIDAARSALRLGATVRIVYRRSKADMPANLDELKGALEEGIE
                     LICMASPLEIMTQEKQGKHVARAVRIQRMKAGPIDSSGRPTPIPTDKIEDIPCSMVIM
                     AIGEKVEIPGIEVLGVERLKNGRIKADPFSHITSNPKVYAIGDAMLGPATAAEAMGQA
                     KVVAEIIDQALSGKKRFDSLFRCFEYRMEIPANTSKQKMTRPTMLPINARKGNFMEIN
                     LGYTGEQARIEADRCLRCDVREHRREPRGSLVGD"
     gene            complement(9327..9842)
                     /locus_tag="PK105_02420"
     CDS             complement(9327..9842)
                     /locus_tag="PK105_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOU60593.1"
                     /translation="MTKTISNSESLNVESIIDSYKGKNGGLLGALEQAQQNDPKKYLS
                     ETTLREISKRLDIPLSRVYSVATFYSFFSLKPQGEHVIIVCRGTACHTRGSLVLLNEA
                     LSRLRIAEFNEKEENSVTSSDNLITIRTVACFGQCALAPVIMVDGVVLSRMTVSKLVS
                     LLEKIRRGGGR"
     gene            10027..10962
                     /locus_tag="PK105_02425"
     CDS             10027..10962
                     /locus_tag="PK105_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60594.1"
                     /translation="MALNNREDEGISIVRPKWPAKFANQSVDCLGRKLKRIFDQNASS
                     DYFTSDEIRDYSYYERLLIQELEGLDQKISKGKLKPSYIAEYPRVIHADEGKIRACIY
                     IGSFDPFQLTHLTVALRFLASDLSNSDFVVIVPEGSPDTAKPLKTDYAFRYSIAKMQI
                     EGIFDSFIKALDLGMQADTIEIIRRFIAMHSGYTLELTHLIGSDALPIAASLISEDMR
                     IWRKEAQQSKVDYQHSIYVVQRGDIPEMHSYLKALKEEDIQIIIDPFSVEAPSSSDFR
                     TKQAYTIVLPTASIRERMEIIFRYHLHKSWSSDRQ"
     gene            11052..11708
                     /locus_tag="PK105_02430"
     CDS             11052..11708
                     /locus_tag="PK105_02430"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOU60595.1"
                     /translation="MNIQSMKLIFLGPPGAGKGTIARMASERLGIPQISTGDIFRTAV
                     KEKTELGLKVQSILASGALVPDELTIAIVKERLSWPDVRQSYILDGFPRTIPQAEALA
                     SFSKPDSVVDFEVDDGLILFRLTGRRVCRTCGAIYHIVTRPSATEGICDLCGGELYTR
                     NDDREETVKIRLEAYHKESEALIEYYSSQGILMVIDGSPDPETVYDTFISKIGRFLKK
                     "
     gene            11828..13171
                     /locus_tag="PK105_02435"
     CDS             11828..13171
                     /locus_tag="PK105_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU60596.1"
                     /translation="MPGVWKGALVLREVAQEQSSPKLPSYSKDQQSMSFSFEFSLKIL
                     SQDRGLLVDIPEQGLYSYPIDEYSFDTQQISFIFDAIEGDEELLFVGTYSSSFLPPLS
                     NHRGAIVGSVRGRSWNGSFYVQRQDSSVDPGSIFLDIPIEDGALPATLVFPLSETPSL
                     DPNSSSDFPLILLVAGAGRADRDGNNVDVPGKNDSLKQLSNMLRMRNVGTLRYDKRGA
                     GEAYKLEKPGRMTSFSEHVRDLSSVIVAAAAIPGHGRLIVAGMNEGAWIAMAALNDER
                     LASLVDGLAVIDASGQSPIETLKQSLAGLDEDLRKKALDAAQSLVQNGVLIDVPAELA
                     DFFAPSRKDWLATWLAFDPVETIAKVRIPILLIYGENDLQVSWQEFSMLVEARPEAAI
                     RIVPRMNYMLKEVKSEDENYSAFTDPSFRVPSLLADLLASFAKAQPAPQGFILWH"
     gene            complement(13168..14172)
                     /locus_tag="PK105_02440"
     CDS             complement(13168..14172)
                     /locus_tag="PK105_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60597.1"
                     /translation="MMRFLPNNRKESRIKNTSIADIILLAAICISVVSCSSVPKEIAI
                     AGIFDRFEKTPHVILRSGDIFLRDIAACLDDSTIQAIETIASDKSNESEKKKEPSKPI
                     DHANIDKLLTRAQVVGIGVSLTETGQPALEAVFAGNFPDLLTSLSFSLDSNWKKINGG
                     YALKSGSLYVRNPNQGQVHLATWVPPASPITNPGASALAAKSGVLANAMDFAIYIDAK
                     STIVSQLPIMEGVTLPFEGILMSAIREKTSPPKGDPSAMYRTTFQIRMKDEQSARTYK
                     PIVRFLWVMLSSKLSSYGIAISPEIAIEQQGSIFISQPVMISARQLVDVGLSISTFDN
                     "
     gene            14416..14865
                     /gene="ndk"
                     /locus_tag="PK105_02445"
     CDS             14416..14865
                     /gene="ndk"
                     /locus_tag="PK105_02445"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOU60598.1"
                     /translation="MAAERTFAMLKPGVLSRRVVGEIISKIERKGFKIIGLKMMNVTI
                     ELAERQYAEHKGKSFYLDLLEYIVSGPVVVMVLEADQAIKLFRLLCGFTSPEQALPGT
                     IRGDYAMHTSINIIHASDSKESAVREIDLFFRPEELIDWEDGNQKWI"
     gene            14947..16752
                     /locus_tag="PK105_02450"
     CDS             14947..16752
                     /locus_tag="PK105_02450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOU60599.1"
                     /translation="MTSTVEVPCWNLDDVFRGFESEEYQKAKNELKSLLETLKRDLTD
                     AKPPSDAKGTANWLFELLSRIDRLETLADTLSAYVQARFTTDTRDSLVIQELGKVEEL
                     LVPATTILVLFRNMLARYEKRIKKAISHDARFVDYAFVLEEALMFQKHQMLPQLEDLA
                     ADLGRSGADAWSRLQDSILANASAVWNEATGERKTMVELRNLAYDPDRNIREKAYRLE
                     LSIWKTYEIPVAAALNGVKGTVLTLNKRRAWESAIDASLAQARISMATLEALIATMEE
                     SLPMWRRYLCAKARLLGLDRLSFFDLFAPLQNQGAVIQSYTWSDAKEFIVRQFGTFDK
                     EMASFAQRAFENRWIDALPREGKVGGAYCTHFPAAKQARVLCNFDGSYSSLITIAHEL
                     GHAWHYENIKDLPSLLSQYPMTLAETASIFSETLVSNAAMATMGEDARVPLIELHLQD
                     ACQVIVDILSRYYFEHDVFKERAKGEITPDRLSDLMLQSQAKTYGEPLREDERHPYMW
                     AVKGHYYIPGLSFYNFPYAFGQLFGMGLYSIYKEEGQSFAAKYRELLRFTGSLPAAEV
                     ARRAGFDIERPEFWQGAMKTFEHEVEFLEAQSNTR"
     gene            complement(16768..18354)
                     /gene="lysS"
                     /locus_tag="PK105_02455"
     CDS             complement(16768..18354)
                     /gene="lysS"
                     /locus_tag="PK105_02455"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOU60600.1"
                     /translation="MEQNRDIHWADQTAKKILDTWGEKDLYTCASGITPSGTVHVGNF
                     REMISVELVVRALRDRGKKVRFIYSWDDYDVFRKVPLNMPNPELLEKYLRFPIIMVPD
                     PWGRDESYARHHEVDVEKVLPEVGIYPEFIYQAQRYRSGTYASSMRRALEMREHLKTI
                     LDKYRDEDHKIQGEWWPISVFCSNCNRDTTEIDDWDGEWGLSYHCETCGFLEKGDLRT
                     LKGAKLLWRVDWPMRWAYEKVDFEPAGKDHHSQGGSFDTSKHVVEEVYGRKPPVTFRY
                     DFIGIKGSPGKMSSSKGKVVDLQDLLRVYQPEIVRYLFAGTRPNTEFVISFDLDVIKI
                     YEDYDRTERIYWGVEKAKNEEVEALERRIYELSQVDRVPEEMPYQIPFRHLCNLLQIH
                     LGNIDAVLSLLPGLKPSQKAHVERRAQCAWYWITECAPEDFRFFLRKPGELASLPEAE
                     AAALRRLRDEVVEKLDTYATENDIAEAIYAAAQSSNIEPKRLFIAAYQALIGKDQGPR
                     LAGFLKTLGKEKVLSILKPY"
     gene            complement(18444..19541)
                     /locus_tag="PK105_02460"
     CDS             complement(18444..19541)
                     /locus_tag="PK105_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HOU60601.1"
                     /translation="MYTLAEVAHVAITQCLDVRKSEKLLIISNPETEQEEIARAFAEE
                     ASSMGAQVQFLLQPVKTQEDFAEDKVINAIEESPDVVLSISTEKLGKDKARLASPLLA
                     PDGRSYDHIFNYLLHGLRALRAVWTPGITKDIFLRTVPIDYEEMRRNAAELASLLDQA
                     STVLVRSPGGTDIVFSVFGRKTMLDDGDFRYPGKGGNLPAGEVFISPALKSAEGLIVF
                     DGSIADIEKDIVIEEPIVCTVRGGYVIGIEGGSEAKKLEAALMRGLSMATGLAGATGM
                     APETALLYGTNARHLGEFGIGLNSAARISGNMLEDEKVMGTIHFAIGSNYDEDAPALI
                     HLDGLVRSPTVVLLMLSGEERTIIEQGVLAV"
     gene            complement(19541..20560)
                     /gene="tsaD"
                     /locus_tag="PK105_02465"
     CDS             complement(19541..20560)
                     /gene="tsaD"
                     /locus_tag="PK105_02465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOU60602.1"
                     /translation="MHILGIETSCDECAAAIVEDGRHILSNVIMTQIPLHERYQGVVP
                     EVASRAHIEWIMGVVRRAISEAGIEICALEGIAATAMPGLIGSLAVGLSFAKALAWSM
                     KLPFKGVNHMLAHLYAPQLIEPIDYPFIGLLVSGGHTVLCRVEDFDRIEVLGRTIDDA
                     AGEAFDKVAKYYGFGYPGGAAIDKLAENGDEKAFKFPMPSLHKGEHRYDVSYSGLKTA
                     VIYHLSSYLRPGAEITPENIAASFRKTAIDILISRLYRAIHDTGIHTVVVGGGVAANS
                     YLRRKLEEEQDLHVYFPPLSLCGDNGAMVAGIAWHYFRRGEIDGWNLAPNSRFADFKW
                     HKEGN"
     gene            complement(20541..21605)
                     /locus_tag="PK105_02470"
     CDS             complement(20541..21605)
                     /locus_tag="PK105_02470"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOU60603.1"
                     /translation="MRKKRTHKGSYYVLLLSSAVLLVIAALIISNSIKSKDSLSKSQD
                     VPYGTPEYVFELPPGYEDLAVIPPEERKGTTSVSKRTASDWDIQAEAQEEIEKVESEE
                     HEPYGSGGPLSVLPGSTTQPTLIIVIDDVGYNNGELDAFLHLPFPITLAVLPQLTHSK
                     DSVIAIHEAGKELILHQPMEALGGNDPGPHAVYCSMDEAIIEKTIAENIDSFPYPPAG
                     MNNHMGSAVTRDSNCMIPILKLAKERGIYYLNSLTAPGTVCAELSRKIGTQYMERDVF
                     LDNNGDRESILAAIEEGKQIARQKGAAVMIGHVWSSKLASTLMEIYPELVEQGYSLST
                     ISQYMKMQAKENSDNAYSGD"
     gene            complement(21637..22458)
                     /locus_tag="PK105_02475"
     CDS             complement(21637..22458)
                     /locus_tag="PK105_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOU60604.1"
                     /translation="MTIADNDENLELYFESIRKIPLVTRQEETILASKIAKGDKCALD
                     RLVEGNLRLVVRIAKSMWSPPHSIMDLIQEGNIGLVKAAERFDPSKNVKFSTYAVWWI
                     RQAISRSLVNTGRHIRLPHRKENALRRLSAIQMRASLDLSRCPSEKELAAETGWKETD
                     ISAILNLGDQPVSLEQTNNDELSILDVYEDWRYNPEREIEKSSHTWQSAWLLSTLSER
                     EQEVISKRFGLGCGPEQSLKAVGSIVGCSAETVRHIEKRALESLRHTALQVGLTA"
     gene            complement(22693..23898)
                     /locus_tag="PK105_02480"
     CDS             complement(22693..23898)
                     /locus_tag="PK105_02480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU60605.1"
                     /translation="MKFVGHSENSVLEADKILNKPLVSVLSGEDFIRKQPPKRSKRAN
                     SKSSNQKQRPSSGPLFSPAVVNSDLNSSREKASDDLQRSTRNQFQNLIKLTPRSILLI
                     AVLLSTSVAGVLAASLISHSHPNISLPDENSAQEALMAMLEPEPSSSPIDGSDLPPIP
                     TLLAEKSYTIRRGDTLQTIASRFGVREDTIISANNLSSKSQIQVGKTLKIPNMNGVYH
                     TVKKNESLSYISRAYGVDMTRIADANNISSATIRIGERLFIPNAKLNASVLRNFYGET
                     FIWPVRGQISSPFGYRTNPFSGQRTFHAAIDIVVNRGTAVKATREGKIADTGYNAVFG
                     NYVIIRHADGYQSLYAHLDSILSKKGTSINQGETIGRSGNTGQSTGPHLHFAIFRNGQ
                     AVDPRQYVK"
     gene            23995..24423
                     /locus_tag="PK105_02485"
     CDS             23995..24423
                     /locus_tag="PK105_02485"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOU60606.1"
                     /translation="MKRSSRPEAKKLDSVMITILVVIFMAFLVRFFLLDAAVVEGKSM
                     LPHYRNGQVVLILKAAYGIRGLNGNYWMQWRQPTTGEVVAAISPVSHEIIIKEIGEVR
                     REGKELAYFLIGYNPIESIDSRDFGLIRFDSIIGKVIPQR"
     gene            24429..26303
                     /locus_tag="PK105_02490"
     CDS             24429..26303
                     /locus_tag="PK105_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOU60607.1"
                     /translation="MDNSAQARFRRRILVLVALLTLFAGVVLVRYGLLALEGPENKGL
                     SAEVPIERGRILDRNGRLLAFDIPKFNLAIRKNEIDPYRISKDLSTVAKSIGVQSESL
                     EKKIRESSQNFIYLAKRLDIDMAKPIQAKIEKGDLAGFVLEEINGRIYPEGRLASHLI
                     GFTGEGNIGLEGVEFKLNKELSGVGSDKPRGNDVYLTIDAPLQYALEGIARKAFEQNK
                     AESIFLLAIDVNSGEVLAYVQMPDFDPNTFGSFRESEREDRMSVYSYEPGSVFKIFSM
                     SSILDAGLITPRTRFNCDGAYRRTLPSGEKIVIKDLNSYGMLDLAGILAKSSNAGAGY
                     ASDRISEDEFYSRILSFGFTEKTGIGSPGENPGNLKEPSKWSARTKPTVAIGQEIRVT
                     ALQMITASVAIANGGILLKPNTISRIEDANGNEVYSHEVIAVRRAIAPETSRAIIAAM
                     ESAASLEGTGWRAKVPDVRMAVKTGTAQMIDLKTKAYSDTDFIASTLGILPANNPKLA
                     IYVAIVKPKGESYLGGQIAAPVLRDAAEAAISTLGLPREKSLNVNHAGVVVIQEPPSA
                     SIGSIMPDLKGYSKRQLLPLLLRKDIEVRLEGDGYVVSQSPSPGTAVEAGTRIMLRLQ
                     "
     gene            26338..26901
                     /locus_tag="PK105_02495"
     CDS             26338..26901
                     /locus_tag="PK105_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU60608.1"
                     /translation="MTRDEAVALWKRWNSDASLWHHALSVEAVMRHFAQKYGENAEYW
                     GLIGLLHDVDYEKFPEEHLRHAREILTSAGFDDEFVRSVESHGWGICTQIAPELRMEK
                     VLFAIDELTGFIAACAYVRPSRSVLDLEVKSVKKKWGSAAFAAGVRRDVIEQGADMLG
                     IPLDELIGETILALRSVASEIGLKGNL"
     gene            26943..>28259
                     /gene="ffh"
                     /locus_tag="PK105_02500"
     CDS             26943..>28259
                     /gene="ffh"
                     /locus_tag="PK105_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOU60609.1"
                     /translation="MLEKISETFTDVFRTISGKSAISEKNVEDALDRIKIALLEADVN
                     VRIVRRFVNGVLEEARGEKVLRSVTPGQQFIKIVYDRMVSLLGDEKQDLLLRGTDTQS
                     VILLLGLQGSGKTTTAAKLANFLKKRGRRVLLVACDLVRPAAVEQLAVLAGQVGVDIH
                     REELKDPVQVARNALVRAKREGHDVLIVDTAGRLQIDEPLMQELARIRDALSPVESLL
                     VADAMTGQSAVEIAKAFDERIGITGAILTKFDSDTRGGAALSLKSITGKPIKFIGVSE
                     RIDGLEPFYPDRIANRILGMGDIVSLVEKAQEVYDQKEALELEQKISKESFTLEDYLG
                     QIQKMKKMGSVKSMLEMIPGLAGQVDEDRIDLQEMKYEEAILLSMTKKERQNYLIIGP
                     SRRTRIAKGSGTSVAQVNRLLKKFEKSRLMMKKMVKNKGAMAKMFGS"
BASE COUNT         7569 a         6270 c         6662 g         7758 t
ORIGIN      
        1 cgatcagttt tatttaaaaa gtgcggagga gatggcatcg atttttgccg aggttcccga
       61 agccattgcc aatacgcttg taataaacga gatggcaaag ctcaagatgg agtttccagg
      121 gcctcttttg cctaattatc aagtgcccga agaattctca tctccagaag aatatctgcg
      181 ccatatagct cgacaaggct ttcagagtcg ttatcccaat ccaaatgccg aggccattca
      241 taggctcgaa tatgaactag acataattat caaaatgggg tttacgggat atttcctgat
      301 agtatgggat tttatcagtt gggctaaaga gcatggtatt ccggtaggcc caggtcgcgg
      361 ttcaggagct ggatctatcg tagcttatgc catgcgtatc accgatatcg atccattaaa
      421 gtatgatctg ctttttgagc gtttcctcaa tcctgagaga gtatccatgc ccgattttga
      481 cgtggatttt gacttcgaac ggcgcggcga ggtgattgac tatgttactc ataaatatgg
      541 cagcgataga gtcgctcaga tcattacatt cggtacactc aaagccaagg ctgtaatcaa
      601 ggatgtagcc cgtgccctgg atattccctt tgacgaggcg aatcagatag ctaagcttgt
      661 gcccgaagat cccaagatga atttacagaa ggctattaag aaggagccga agcttgccac
      721 tcttgcacaa aatccttctt acttcgagct cttctcgata gccgctaagc tagaaaacct
      781 tcataggcat agctcgatcc atgcagcggg tatcgtgatc ggcaagacag atttgatcga
      841 ctatgtgcct ctatataagg atccaaagac tgggctcatt gcgacccaat atactatgga
      901 atacctggaa gcttgcggtc ttgtaaagat ggactttctt ggccttaaga ctctgacctt
      961 gattcgcaat accatggaac tattgagaaa gagaggtata tacctcaaag aggaagatat
     1021 accagaagat gatcccagga ccttcaagat gcttggcgaa ggcaaaagca cttcgatttt
     1081 tcaatttgaa tcacagggaa tgcagtctat tctcaagcga gccaagcctt cgagtattga
     1141 agatctcata gctctcaacg cactctatag accgggtcct atggaattta tagatcagta
     1201 tatagattca aagaatggta aaattgctat tagttatccc catccttcgc ttgaaaaata
     1261 ccttaaaggc acatatggag taatcgtata tcaagagcag gtgatgcaag ttgcacgaga
     1321 agtggcggga tattcgttgg gacgcgcaga ccttcttaga agagccatgg gcaagaaaaa
     1381 gcccgacata ctgcaaagag aagaagctcc attcatcgaa ggtgctctta ctcgcggcta
     1441 ttcaagagaa gaagcaaagc ggattttcga tattctaacg ccttttgctg gctatggttt
     1501 caataaatcg catgctgctg cttactcagt aattgcatat cgtacggcct acctaaaagc
     1561 aaattattct gttgagttta tggccgcgaa cttgacaaac gagatcagta acaccgacaa
     1621 gttgaccgtg tacataagtg aagctcgaca gatgggtttg tctgtgctgc cccctgatat
     1681 caatcgttca gaagccaagt ttacggtcga taatggacag atcatatatg gatttcttgg
     1741 tatcaagggc gttggcgagg ggatcgcaat ggagattcaa aaagaaagag agtctcatgg
     1801 cagatttata gattttcttg attttcttac gcggatgaat aatgcgggca taaatcgcaa
     1861 aacgatggag tcgctcatta tggcaggctg cttcgattcc ttgaagaaag gccgccatga
     1921 gcttatgggc aactttgagc gtgctatgga atatgtgcat gagaaatcga gttttgaagc
     1981 ttcgcgttca gggatgctgt tccaggagga tgatctggga atttatccgg attttgtttt
     2041 tcaacctttt gaagaattct cgaatcaaga aatgttgcag gcagaaaagg agttgttggg
     2101 ttttttcttt tctgcgcacc caatggatga gtaccggatt atttgggaga gatcctcgac
     2161 acttgatctt gggcatctgg atcaggcaag cacaaaccgt gaatatatag tggtcgcgct
     2221 tctcaaggaa tttcgcattt atacaaccca gaatggacgg cgaatggctt ttggcaagct
     2281 tgaggattac aatggttcca tagaaatggt cgtgtttcca gatctgtttg agaaaaatga
     2341 agcgtctttt gtgaaggatc gtgtcctttt ccttaaagga gtactggatg actctcgaag
     2401 ggatttttcg ttaaaggtgc aagctatcct ggatgcggct tctcttaaga ataattcctg
     2461 gagagaatta cacatcaggt ttaagcgagc agccatcgaa gaagcagatc tctatccgct
     2521 aagagatgcg ctatattcta tccatgggca gtgtcgggtc atttttcatg tgcccatgaa
     2581 aggaggagca gagactctga tcgaagctgg agctcataca aagtgctccg cgagagatga
     2641 ggatatggcg tttatagaaa gacaggctgc agtggcggag gtatggcgca aatgaaaatt
     2701 aagatacttg gcaatggcgg cttctacaat gaaggattgg cctataatgc gctcgctata
     2761 gatggccatg tgctcgtgga aactccgcca gacattctcc aatcccttca tagtcaggga
     2821 atgaagcctt ctcagataga tacggtctat ataagccata atcatggaga tcactgtttc
     2881 ggctttccgt tcttcttttt taattggcta tatgcaggag gttcggattc tcattatgaa
     2941 aaacagacag agctagttat aatcggaccc gaaggactaa aagagcatct caaagctctc
     3001 atgcgtctag ctattctgcc cgagcatcaa tatctattcg attttgacca aagggtcaag
     3061 tttgtcgaga tagacgaaag atcgattgta tccactagcg gtgggctatg gtttggcttt
     3121 ttgcgcacaa agcattttgt gcccacattt tcgcttgtgg ttggtaaaac tggaccggat
     3181 aaagggattc cttcttgtaa cgactatctc gagaatgcaa tatttatata tagctcagat
     3241 acttccatgt tcgatggcat atataagtta ttgagctcaa gcgcaaggct catcctttgt
     3301 gatacgaatg gcgaagaaga taatggcgtc catatgagcc caagagaact tatggcagcc
     3361 gagaatcaag ttaggacaag gagctctgag cgtgcagacc tttcagagta taggctaagc
     3421 ggggaaaacg acaaggatcg cgtattggga attcatctaa gcaagaagat ggatagggcg
     3481 caaggactca agttcgccca gcctggcgaa gaatactgca tcgagtagat gcttttataa
     3541 agaagcttca aggatttaat caatatgctt aaccacccac tccgtcagct cttcccaatt
     3601 cgaaaaggaa agatctgcat ctgagttttg tcttccacgc aaggctgctc gcattccagc
     3661 ttttttagcg cctaggatat cgtactcgac attattccca acatagagga tctcagaagg
     3721 ctggatgcgt atagtctgga ttataccgtc gaatgcacgc tgatggggtt ttagaacacc
     3781 ccaatcttct gtgcagagct taaaatcgaa taaatcatca aggccgaggg atttcaattt
     3841 attttgtgga ggcatatcgg aaagggcagt gattgttatt cctctctgtc gaatcgcttc
     3901 gatcgcttgc cttgtcccct tataaggacg gatcaatgag aaacgctcag gcacaagatg
     3961 atagaagatt ctttctatgg attgaccaat ttgaagctcg tcttttctga gacgctccgc
     4021 aacaagcgat atctgaaaac tgtgaagctc ttccatgttt cgaggaatat agttgggttg
     4081 tgcttgaaga aatctgattt cttttctcac ctctgagaat gccatgaata tgcggggatg
     4141 gctaagaaac aggtcgatgc atctcagcgc gattgaatat tcgggataaa gagtaccatc
     4201 gacatcaaat gccactgcgc gtaggcgata ccgtccttcc ataagcaact ttatatatga
     4261 agcagaggag ggctgtctat tacctataaa gccaatagat cttcgcagtg gcggcggccc
     4321 cgaatatgaa ataaaagaat gccttgaaaa tcttagaata ccctcgcctt atagctggta
     4381 tgcaatagtt catgggacag gtggccaagc ggcttgccta aaaattcctt atatagtgtg
     4441 actattgcgg gattttcgtg agattttcta attggaagtc tgcgatcctc ttcgtagagc
     4501 gataggctac gggctctttt cttttctata tctgcaagta caggctgtcc gcctccgcca
     4561 acgcagcctc cggggcatga catgatttca atgaaatgat aggggctctt tcctgctcgg
     4621 atctcgtcca gcaaaatgcg ggcatttgaa aggccatggg caacagctac gcgaacagga
     4681 gagcctccaa gcacaacttc ggcacttttt atcgaatcga agccacgcac ttggacaaaa
     4741 tccatgttct gcaaaggttt cttttcgaca atctcatata cagtgcgcaa ggcagcttcc
     4801 attacaccgc cggtagttcc aaaaatggta gccgcaccgg tcgatttgcc cagaggatca
     4861 tcgaaatcct cttgtggcaa ggctgtgatg tctatacccg caagccttat catacgggca
     4921 agctctctag tggtgagagc ccaatcgaca tcgaagaatg ggcgcaatcc ggtggtttcc
     4981 gggtcttttt ccttccacca gtcccatgcg gagtccattt ccttgcgtct agcttcatat
     5041 ttcttggctg tacagggcat tatagagact accctcatat ttttgggtgc tatattggca
     5101 ttctgtgcat aatatgtctt agcgactgcg ccaaacattt gctgagggct tttgcaggta
     5161 gagacatgat tgagcagatc aggatagaag ccttcgataa aatttatcca tcctggtgag
     5221 caggaagtga tcatgggcag ggttccgcca tgagaaagcc gctcaagcaa ctcgtttcct
     5281 tcttcgatta tggtgagatc agccgcaaac tgtgtatcga agactcttga gaatccaagt
     5341 ctacgaagag ccgcaaccat ctggccggtt acaaggcttc cagcgggaag gccaagagct
     5401 tcaccaagcg atgcgcgaat tgccggggcg gtttggacta ccactgaaag cctcgaatct
     5461 ccaagcgcgc catagacatc ctgcgattcg tccttctcgg ttatggcgcc ggtcgggcat
     5521 accaaggagc attggccaca ttggacacag gcactttgag ctaatcctct atccataaaa
     5581 gttgcgactc tggttcgagt accgcgcccc gtaaaggcaa tcgcgcttac gccctgggta
     5641 tcctcacaga ccgagacaca tcgtccacag aggatgcatt ttctatcgtc gcgtacgatg
     5701 ccttcggaag aattatcggg aggcgggtat tttttgtagc caggaaacct atctgcatgg
     5761 actccgagta aattggccat ggtgtgaagc tcgcaagtgt cggcccttaa acaggaaagg
     5821 cattcatctg ggtggtttgc aagcagtagt tcgaccgctg cttttcgtgc ttgaataact
     5881 cgagggctag atgtccgaat cttcattccg ggagtaggtt tctgcacgca agagcgcaaa
     5941 agggattttg ctccttctac ttcgacaacg catacaccgc aagaggcgtt ttccgaaata
     6001 tccttgagaa agcagagagt cgggatcttg acgccggctc gagcacaggc ttctacgata
     6061 ttctcgtttg aatcgacctt tatggtctgt ccatcgatcg ttatatctat cagattttcc
     6121 attggtgtac ctcctttagt cgccaaccag gctgccgcga ggttcacgtc tatgttccct
     6181 cacatcgcag cgaagacagc gatcagcctc gattcgcgct tgctcgccgg tatagccaag
     6241 attaatctcc ataaaattgc ccttgcgtgc attgattggg agcattgtag gtcgagtcat
     6301 cttctgcttg ctagtattag ccggaatctc cattctgtac tcaaagcatc gaaataggct
     6361 gtcgaagcgc tttttcccgc ttaatgcttg gtcaattatc tcagcaacga cttttgcctg
     6421 tcccattgcc tctgctgccg ttgcaggacc gagcattgca tctccaatgg catatacttt
     6481 tgggttggat gttatatgag agaaggggtc agccttgatg cgcccattct tgagacgttc
     6541 tacgcccaaa acttctatgc caggtatctc tactttttca cctatagcca taataaccat
     6601 agagcaagga atgtcttcga ttttatctgt aggaataggt gttggacgtc ctgaagaatc
     6661 gataggccct gccttcatac gctgaattcg gacggctctt gcaacatgtt tcccttgctt
     6721 ttcctgtgtc atgatttcca gcggcgaagc catacagatt agctctatac cctcttcaag
     6781 ggcacccttg agttcatcca ggttagctgg catatctgcc ttcgatcttc gatagacgat
     6841 tctaaccgtt gccccgaggc gtagagcaga gcgagcagca tctattgccg aatttcctcc
     6901 gcctattaca actacatcgc ctcccgtatg aaccttttct tttttcgcaa gcgcattgag
     6961 aaaatcacag gctttgtaga caccgatagc gtcttctccc ggtatattca tggtaagttc
     7021 ggatgaagcg ccagcagcta ctattatggc atcgttctct tcatagagtt tcttccactg
     7081 ttccggctcg gttatgcgtg tattgaatac aaagcgagtt cccaattttt ctatgagatt
     7141 gatctctttt tttagtatat cctttggcaa tctatatgat ggaatacccc agcgaagcac
     7201 gcctcctgcc tcttgtctag attcatagat ggtcacttca tgaccaaggc gaaccaagta
     7261 gaaagcggcc gtcaagccag cgggtcctgc tcctattatg gcaattttct tgccgctagg
     7321 gggcaatttt tctttgagaa gcttcgtata tacttgtttt tctctgccca tcttatacat
     7381 ggtgtcggca agatagcggt gaatctctcc ctgtgataca ggttcgtcta gcatgtcacg
     7441 gcgacagcgc atccggcaat gaaagtggca gatacgtcca attgtgccgg gcaaaggatt
     7501 ttcccgcaga gtgagctcaa aagcatcttc tatacggttt tcctttagga gctgcaagta
     7561 tcctggaata ttcatgtgca aggggcagga ggattcgcaa agagcaatgt ataggttttc
     7621 gcaaacgcca gcagggcagc gtttcccttt tatgtgttct atatattcat ctctaaaata
     7681 gcgcaaggtt gtaagaacag ggtttgccga ggtttgccca agaccgcaga gagaagagtc
     7741 cttgactatt attgcgagtt cctcgagcgt gtcgagatct tcctcactcg cttcaccatc
     7801 ggataccttt tcgagaatat gcagcatttg ggaagtacct tctctgcatg atgtgcattt
     7861 tccgcaagac tctcccgata caaagcctgt aaaatatctg gctacatcga ccatacaatt
     7921 atcttgatcc atcaccacca tgccacccga tcccataatt gccccgagct cattaaggtg
     7981 ttcgtaatcg ataggagtct tgaataggct cgctggcaca caacctccgg atggtccacc
     8041 gcattgtaca gccttgattt ttctcttcgg gcccatgcct ccacaaatac catagattac
     8101 gctttcaagc gtgctgccaa gaggaagttc gacaaggcct gtattccgaa cttttccgac
     8161 aaaggaaaac acctttgttc ccgtactcat aggagtacca aaacccgcaa accactctcc
     8221 tcctctggct gttatcactg gtatgttgca ccaagtttca acattgttta tcgaggttgg
     8281 acgaccataa agacctttct ccactgggaa tggaggtctt ggtcttggtc ttccagcatt
     8341 gccttcaata gaggctatga gtgcggtctc ttcaccacat acaaaagctc cagctccggt
     8401 cactaaatct atatcgaacg agaatttgga tccaagtatg ttctttccaa gaagtccatg
     8461 ctgccttgcc gtttctatgg ccaaattgag cctatagaca gctaatggat attccgcacg
     8521 gacatagaca attcctttgc ttacattcat tgcataggcg ccaagtagca tcccttccaa
     8581 caacatatga ggatcggatt cgatctcatt tctgttcatg taagcaccag gatctccttc
     8641 atctgcatta cagatcaaat aggcttcccc aggattatcg ataagcgatt tacgcatgag
     8701 cgaccatttt ttccaggttg gaaagcctgc accgcctcgt ccgcgcagcc cggatttttt
     8761 cacttcttcg atgatagcct ctggtggcat ggaggtcaga gctcgaacca gggctccgta
     8821 tcctcctacg gcgatgtatt cttcgatgca ttccgggtcg atcattccgg catcgcgtag
     8881 aatgatcttt tcttgatccc tgaagaagtt gatatctttc catgttggga ggaaatcata
     8941 accttcgcca aaatccacct tagatgtcct gaagtcccat tcttcgatct tagcttctgc
     9001 catttttgca atcttttcat atccgagatc ggatgaaaga ccagcaaaaa tacgcgatgc
     9061 tttggatgta ttgacttctg taaagaaaag aaccggcttt cccggtctcc atgtcattac
     9121 aacgggttca gctgcacaaa aaccaaagca tccaacacga cgcagacgaa tattggtatt
     9181 ctctgtcttc tgagaaagag tctcccagac cttatcggct ccagagccta tgccacaggt
     9241 tcccattccc acagaaatcc aaggagtatc gccatgaaaa tggctaaagc cttcctgtgc
     9301 tagaaatccc aaatcggtgt tcttgttcat cgcccacccc cccttctgat cttctccaga
     9361 agagagacga gcttactgac agtcattcgc gatagcacaa caccatcgac cattatgacg
     9421 ggtgcaagag cgcattggcc aaagcacgct accgtgcgaa tcgtgattag attatcgctg
     9481 ctcgtcaccg aattttcttc tttttcattg aattctgcta ttctaagtct ggagagagct
     9541 tcgtttagga gcacaaggga gcctcttgta tggcatgctg taccgcggca gacaataatg
     9601 acatgctcgc cttgcggttt caaactgaag aaagaataaa atgttgccac tgaatatact
     9661 ctagatagag gtatatcgag tcttttggag atttccctta gtgtcgtctc ggagagatat
     9721 tttttggggt cattttgttg tgcctgttcc aaggctccaa gcaggcctcc atttttgcct
     9781 ttgtatgaat cgatgatgct ttcaacattc aaggattccg aattggagat tgtttttgtc
     9841 atgctacctc ctttgacatc ttttattatt ctatatcgat taggatattt gtcaagaaaa
     9901 atttatctat aaaatcataa ttatagaaca ggcaaattcg aatcataatc attgtatctc
     9961 aataaatagt aaaatatcga taaaaagagt attgcatcca gtctggtttt atacaatagt
    10021 atagatatgg ctctgaataa ccgagaagat gaagggattt ctattgttcg tcccaaatgg
    10081 cccgctaaat tcgccaacca gagtgtagat tgtttgggtc gaaaattgaa acgcatcttc
    10141 gatcagaatg ccagttcgga ctactttacc agcgatgaaa tcagagacta ttcctactac
    10201 gaaaggctgc tcatacaaga gcttgaaggc ctagaccaaa aaatatcaaa aggaaagcta
    10261 aagccaagct atattgcgga atatccaagg gttattcacg ccgatgaagg caagattcgc
    10321 gcttgtattt atattggttc attcgatcct tttcagctta cccatctcac cgttgctctg
    10381 cgttttcttg cctctgatct aagcaactca gatttcgtag ttatagtacc cgaaggttcg
    10441 cctgatacag caaagcctct taaaaccgat tatgcttttc gctattcgat agcgaagatg
    10501 cagattgagg gtattttcga ctcctttatt aaggcattag atctaggcat gcaggctgat
    10561 acaatcgaaa taataagaag atttatagca atgcacagtg gatacactct cgaattgaca
    10621 catttaatag gttcagatgc gcttccgata gctgcaagtc tgatttcgga agatatgcgg
    10681 atatggagaa aagaagctca gcaatcaaaa gtagactatc agcattctat atatgttgtg
    10741 caacgaggag atataccgga aatgcactcc tacttaaagg ccttgaaaga ggaagacatc
    10801 cagatcatca tcgatccttt cagtgtagag gctccctcga gcagcgattt tcgaaccaaa
    10861 caagcctata cgattgtatt gccgactgct tccattagag aaaggatgga gataatcttt
    10921 cgttatcatt tgcacaaaag ttggtcttcg gatcgacaat aatatcgaag aaattgcctg
    10981 atttggatgc agatgaaaaa aaggcacgac aagctctttt ttcgagcttt gaagtgttgt
    11041 ataagtattt tatgaatatt cagtcaatga aattgatttt tctcggacct cctggagcag
    11101 gcaaagggac aattgccaga atggcctcag agaggctggg tattcctcag ataagcacag
    11161 gtgatatatt tcggacagcg gtaaaagaaa agactgaact tgggctgaag gtccaatcga
    11221 tacttgcctc tggcgctctg gtacccgatg agttgacaat cgccatagta aaagaaaggc
    11281 tatcttggcc ggatgtaagg caaagctata tcttggatgg cttcccaagg acgattcctc
    11341 aagcagaagc gctagcctcc ttttcaaaac cggattcagt agtggatttt gaagtagatg
    11401 acggtttgat cctctttcgg cttaccggaa gacgagtatg taggacctgt ggagccatct
    11461 atcatatagt tacaagacct tcggcaactg aagggatttg cgatctgtgc ggaggtgaac
    11521 tatatacgag gaacgatgat agggaagaaa ctgtcaaaat tcgactcgag gcatatcaca
    11581 aggaatccga agccttgata gaatattatt ctagccaggg aattctaatg gttattgatg
    11641 gcagccctga tcccgagact gtctatgata cttttatttc aaagattggg cgattcttga
    11701 aaaaatgatt atttcgggat ctcggttccg gaagacagcc cgattttttc cgagcctttg
    11761 gtttttgttt tttatcttga ctattttctc aatcttgcct gtcaaaagtg caggaacaga
    11821 ggagattgtg ccaggggtat ggaaaggtgc gctagtattg cgagaagttg cacaagagca
    11881 gtcttcgcca aagcttcctt cctattcgaa ggatcaacaa tcgatgtctt tcagttttga
    11941 gttttccttg aagattctct ctcaggatag aggattattg gtcgatattc ctgaacaagg
    12001 cttgtattct taccctatag atgaatatag cttcgatact caacagatca gctttatttt
    12061 tgacgcgata gaaggagatg aagagctact ctttgttggc acttactcct catctttttt
    12121 gcctcctctg agtaatcata gaggagccat agttggctcg gtcagagggc gatcttggaa
    12181 tgggtcattc tatgtacaaa ggcaagattc gtcagtggat ccgggttcga tttttttgga
    12241 tatccccata gaggatggag cacttcccgc cactctagtc tttcctttga gcgagacccc
    12301 ttctctcgat cctaattctt catctgattt tcctctcata ttgctagtag ctggtgctgg
    12361 cagggcagat agggatggga ataatgtgga tgtacctggg aaaaacgatt ccctgaaaca
    12421 attgtccaat atgttgagaa tgcgcaatgt tggaactctt cgctatgata agcgaggtgc
    12481 aggcgaagca tacaagctcg aaaagccagg tcgcatgacc agcttttctg agcacgtcag
    12541 agatcttagc tctgtaatcg tcgctgccgc agctataccc ggacatggaa ggctgatagt
    12601 agctggcatg aacgagggag catggattgc catggcagct ttgaacgacg aaaggcttgc
    12661 ctcacttgtc gatggtcttg ccgtgatcga tgccagcggt cagagtccga tagagacctt
    12721 gaaacagtct cttgcaggct tagatgaaga tcttcgaaaa aaagcattgg atgcagcgca
    12781 gagcctggtc caaaatggag ttttgatcga tgtcccagca gagcttgcag attttttcgc
    12841 accgagcaga aaagactggc tggcaacttg gcttgccttt gatcctgtag agacaatcgc
    12901 aaaagtccga attccaattc tgctcattta tggagaaaac gatcttcaag tcagttggca
    12961 ggagttttcc atgcttgtcg aagcaagacc ggaggctgcc attcgaattg tgccgcggat
    13021 gaactatatg ctaaaagaag tcaagagcga ggacgaaaac tactctgctt tcactgatcc
    13081 atcttttagg gtaccttccc ttcttgcaga cctgcttgct tcttttgcaa aagctcagcc
    13141 cgccccgcag ggttttatac tctggcacta attatcgaaa gtcgagatag aaagaccaac
    13201 atctacgagt tgccgtgcgc tgatcatgac aggctggcta atgaagatcg atccttgctg
    13261 ttcaatcgca atctctggag aaatggcaat accataagag cttagtttag aagacagcat
    13321 aacccaaaga aaacgaacga taggcttata tgtgcgtgca ctttgttcat ccttcatccg
    13381 tatctggaaa gtggtgcgat acatagcaga agggtcgcct ttaggaggac ttgttttttc
    13441 ccggattgca ctcatcaaaa tgccctcgaa aggcaaagtg accccttcca taataggtaa
    13501 ctgactgaca attgtagatt tagcatcgat atatatcgca aaatccatag catttgctag
    13561 aactccgctc ttggcagcca gtgcgcttgc tccgggatta gtgatagggg atgcaggggg
    13621 cacccatgtt gcaagatgaa cttggccttg gtttggattg cggacatata gacttcctga
    13681 ttttagagca tagccaccat tgatcttctt ccagttggaa tcaaggctaa aagacaggga
    13741 agttaagaga tcaggaaaat ttcctgcaaa taccgcttct aaagctggtt ggcctgtttc
    13801 ggtgagcgaa actcctattc ctacaacttg cgctctagtc aatagcttgt cgatgttagc
    13861 atgatcgatt ggttttgaag gttctttctt tttttctgat tcatttgatt tatcagaagc
    13921 aatagtttca atagcttgga ttgttgaatc atctaagcat gcagctatat cgcgaaggaa
    13981 aatatctcct gaacgtaaaa taacatgcgg cgttttttca aaacgatcga atatccctgc
    14041 aatggctatc tccttaggca cggaggagca ggagacaaca gaaatacaga ttgctgctaa
    14101 tagaataatg tcagcaatcg aggtattttt tattctgctt tcttttctat tatttggtag
    14161 aaatcgcatc aaggccaaat tcctttcttt ttctttgagt aagctaagca gaatatatca
    14221 cagaattctc aaacatatcg agtaaggaga ttgatgggag attgatggaa aactatatcg
    14281 cgctctttat gataggaaac cagaatcaat tgagctatat gcgtagccct gtgatgacat
    14341 tatgattcaa agaatgctat tttgaatgtt gaatctatat cttatctggc aaaatagcat
    14401 atggaggtat gcgaaatggc tgctgagcga acatttgcga tgctcaagcc cggtgtctta
    14461 tcccggcgtg ttgttggaga gataatctcg aagatcgaac gaaagggctt caaaatcata
    14521 ggtttgaaaa tgatgaatgt cacaatcgag cttgccgaaa gacaatatgc cgaacataaa
    14581 gggaaatctt tttatttaga ccttctggag tatattgtgt caggacctgt tgtggtaatg
    14641 gttcttgagg cggatcaggc aattaagctt ttcaggcttt tgtgtggctt tacaagtcca
    14701 gaacaagctc tccctggtac catcagaggg gattatgcca tgcacactag tatcaatata
    14761 atccatgctt ccgattcgaa ggaatccgct gttcgggaaa ttgatctttt ctttagacct
    14821 gaagagctca tagattggga agacgggaat caaaaatgga tctagggtga gagcctcatt
    14881 tatgaggagg attaattctt gaatatgtta ttaccagaaa atgcacatat caaaggagga
    14941 ggtcttatga caagcacggt tgaagtacca tgctggaatc ttgacgatgt tttccgtggc
    15001 tttgaatccg aagaatacca aaaggcaaaa aacgagctca agagcctttt ggaaacgctg
    15061 aaaagagatc ttaccgatgc taagccgcct tccgatgcaa aagggacggc taactggcta
    15121 ttcgagcttc taagcaggat agatcggctc gagacgcttg cagatacgct ctctgcctat
    15181 gtccaggcgc gttttaccac cgacacaagg gattcgcttg tgattcaaga gctcggaaaa
    15241 gtggaggagc ttctggttcc tgccacaacg atcctcgtgc tcttccgcaa tatgctagct
    15301 cgttatgaaa agagaattaa gaaagccata tcacatgatg ccagattcgt agactacgct
    15361 tttgtacttg aagaggcact gatgttccaa aagcatcaga tgttacctca acttgaagac
    15421 ctggctgcgg atcttggtcg ctcaggagcc gatgcatggt ctcggcttca ggattcgatc
    15481 cttgcaaatg ccagtgccgt gtggaatgaa gcaacaggag aacgcaagac aatggtagag
    15541 ctcagaaatc tcgcttatga tccggatcgc aacattcgcg agaaagccta tcggctcgag
    15601 ctttccatat ggaaaacata tgaaataccg gtagcagccg ctctcaatgg tgttaagggc
    15661 actgtgttga ctttgaataa gaggagagcc tgggagagcg ctatcgatgc ttctcttgct
    15721 caagccagga tatcgatggc gacacttgag gccttgatag ctaccatgga ggagtctctt
    15781 cctatgtggc ggcgttattt gtgcgccaag gcgcggcttt tgggtcttga tcgactttca
    15841 tttttcgatc tctttgcacc cctacagaac caaggagccg tgattcagag ctatacctgg
    15901 tcagatgcaa aagagttcat cgtcagacaa tttggcactt ttgataaaga aatggccagc
    15961 tttgcccaaa gagccttcga aaacagatgg atcgatgcgc ttccccgcga ggggaaggtg
    16021 ggcggtgcat attgcacaca cttccctgct gccaaacaag ccagagtcct ctgcaatttc
    16081 gatggctctt actcttctct tataaccata gcacatgaac taggccatgc atggcattac
    16141 gagaatataa aggatttgcc aagcttgctt tctcagtatc ccatgacgct agcagagact
    16201 gcttccatat tttctgagac cctggtttcc aatgccgcca tggctactat gggagaggat
    16261 gccagagtgc ctctgatcga gcttcatcta caggatgctt gccaggtgat tgtagatatc
    16321 ctttctcgtt attattttga gcatgatgta tttaaagagc gagcaaaagg cgagataacg
    16381 ccagatcgct taagcgatct aatgcttcaa tctcaagcga aaacttatgg agagcctctg
    16441 cgagaagatg aacgtcatcc ttatatgtgg gcggtcaaag gccattatta tattccgggg
    16501 ctttctttct ataactttcc atacgctttt ggtcaactct tcggaatggg gctctatagt
    16561 atctataaag aagagggcca atccttcgcg gccaaatacc gtgagctttt gcggtttacg
    16621 ggctctctac ctgccgcaga agtggcaagg cgcgcaggtt tcgatatcga aagacccgaa
    16681 ttctggcagg gcgccatgaa gacatttgaa cacgaggtgg aatttcttga ggctcagagc
    16741 aacactagat agcctgtaga tcccttatca atagggcttt agaatcgata ggactttctc
    16801 ttttcccagt gttttaagga agcctgccag cctcggccct tgatccttac ctatgagagc
    16861 ctgataagct gcaatgaata gtcttttagg ttctatattg ctggattgag ccgcagcata
    16921 gattgcctcg gcaatatcgt tctcggtagc ataggtatcg agcttttcta ccacctcgtc
    16981 acgcaggcgt cgcaatgcgg ctgcctcggc ttcggggaga gaggcgagtt cacctggctt
    17041 acgcaggaag aacctgaagt cctctggtgc gcattcggtt atccaatacc aggcacattg
    17101 agctcttcgc tctacgtgcg ctttttgcga aggctttaat cccggaagaa gcgataacac
    17161 ggcatcgatg tttcccaggt gaatctgtaa tagattgcat agatgccgaa atggtatttg
    17221 atagggcatc tcctcaggaa ccctatctac ctgagacagt tcatagatgc ggcgttcgag
    17281 agcttcgact tcttcgttct ttgctttttc gacgccccaa tagatgcgct cagttctatc
    17341 gtaatcctcg tagattttaa tcacatcgag atcaaaactg atcacaaact ctgtattcgg
    17401 gcgagtgcca gcaaagagat agcgaactat ttctggttgg tagactctca acaagtcctg
    17461 aagatcgaca accttgccct ttgatgagga cattttgccg ggcgagcctt tgatacctat
    17521 aaaatcatag cggaaggtca ctggtggctt tctgccataa acctcttcga ccacatgctt
    17581 cgaggtatcg aaagaacctc cttgagaatg atggtccttg cctgcaggct caaagtcgac
    17641 cttttcataa gcccaacgca taggccaatc gactcgccat aggagctttg ctcccttgag
    17701 cgtacgtagg tcaccctttt ctagaaaacc gcaggtttcg caatggtaac tcaagcccca
    17761 ttcgccatcc caatcatcga tctcggtggt atcgcggttg cagttcgagc agaacaccga
    17821 gataggccac cattcacctt gaatcttatg gtcttcatcg cggtatttat cgaggattgt
    17881 ctttaagtgt tcgcgcatct caagagcacg gcgcatgctc gatgcataag tgcccgagcg
    17941 atatcgttga gcttggtaaa tgaactcagg gtagattcct acctcgggca gcactttctc
    18001 gacatcgacc tcgtggtgac gagcatagga ttcatcgcgt ccccaaggat ctggtaccat
    18061 aattatcgga aatctgaggt atttttccag taattcggga tttggcatat tgagaggtac
    18121 ttttcggaaa acatcgtaat catcccagct ataaatgaat ctgaccttct tgcccctatc
    18181 tctaagtgct ctgacaacga gctctacgga tatcatttca cggaaatttc caacatggac
    18241 agttcccgaa ggagtaatcc ccgatgcaca ggtatacagg tctttttcgc cccatgtatc
    18301 caagattttt ttagctgttt gatcagccca atgaatatct ctgttctgtt ccatgttgtg
    18361 gaagtataac gatatttatg tttttgtaga atagcggttg ctgatggaaa acttagaagg
    18421 gctcgtattt caatgaatag ggcttacacg gctagaacac cttgttcgat gatggtcctt
    18481 tcttcgccag agagcatgag aagcaccact gtgggtgatc ttactagtcc atccagatga
    18541 ataagagctg gcgcatcttc gtcgtaattc gatccaattg caaaatgaat ggtacccata
    18601 actttctcat cttctaacat attgcccgaa atacgagcag cagaattgag tccaatacca
    18661 aattctccca ggtggcgagc atttgtgcca tataatagcg ctgtctccgg agccattcct
    18721 gtcgcacctg cgaggccggt ggccattgaa agccctcgca tcagggctgc ttctaatttt
    18781 tttgcttcgc ttccgccttc gatgccgata acatagccgc cccgtacagt gcacactatt
    18841 ggctcctcga tgacaatatc tttttctata tccgcaatag acccatcgaa cacaattaaa
    18901 ccttcagccg atttcagagc cggagaaata aaaacctcac cggcaggtag attccctcct
    18961 ttgcccggat agcgaaaatc gccatcatcc agcatcgtct tgcgtccgaa taccgagaat
    19021 acaatatcgg tgcctccagg actccggaca aggactgtgc ttgcttggtc gaggagactt
    19081 gcgagttctg ccgcgttcct acgcatttct tcataatcta ttggcacggt tcttaggaat
    19141 atgtcctttg taattccagg tgtccagact gcgcgcagtg ccctgaggcc gtgcaataga
    19201 taattgaaga tatggtcata agagcggcca tctggagcca aaagaggcga tgcaagcctg
    19261 gctttatctt tgcctagctt ttctgtcgaa atagacagca ctacatctgg gctttcttct
    19321 attgcgttga taactttatc ttctgcaaaa tcctcttggg ttttgacggg ctgaagcaag
    19381 aattgcacct gcgctcccat cgaagaagcc tcttctgcaa aagctctcgc tatctcttct
    19441 tgttcggtct caggattgga gataatcaag agcttttcag attttcttac atccagacac
    19501 tgagtaatgg ctacatgggc aacctctgcg agggtataca tcaattacct tccttatgcc
    19561 atttgaaatc tgcaaaacga gaattcggag ctaggttcca gccatctatc tcgcctcgcc
    19621 taaaataatg ccatgcaatt ccagcaacca tggctccatt atctccgcag agcgaaagag
    19681 gagggaaata gacatgcaaa tcttgctctt cttcgagttt tcgccttagg tatgagttgg
    19741 ctgcaacccc accccccacg acaactgtat ggatacccgt atcatgaatc gcacgatata
    19801 gcctggatat tagtatatct attgctgttt ttctgaagct tgcagctata ttctctggtg
    19861 taatctctgc tccgggtctt agatatgagg aaagatgata gattactgcg gtcttaagcc
    19921 cggaatatga aacatcataa cgatgttctc ctttatgtag agaaggcatg gggaatttga
    19981 aggccttttc gtcaccattc tctgcaagct tgtcgatcgc agccccgcca gggtagccaa
    20041 acccatagta cttagcgacc ttatcaaagg cctctcccgc cgcatcgtct atcgtccttc
    20101 caagcacctc gattctatcg aaatcttcaa cacggcagag aaccgtatgt ccgcccgaca
    20161 caaggagccc aataaatgga tagtcgatgg gttcgatcag ctgaggagca tagagatgag
    20221 caagcatatg atttacgccc ttaaaaggaa gcttcataga ccaggcaaga gcttttgcaa
    20281 aggacaatcc tacagcgaga gaaccgataa gtccaggcat agcggttgct gctatgcctt
    20341 ctaaagcaca tatctctatt ccagcctcgg atatagcgcg ccttaccacg cccattatcc
    20401 attctatgtg agccctgctt gcaacttcag gaactacacc ttggtagcgc tcatggagcg
    20461 gaatctgagt cataattaca ttggaaagga tatgccttcc gtcctcgacg attgctgcgg
    20521 cgcattcatc gcaggaagtc tcaatcccca gaatatgcat tatctgagtt ctcttttgct
    20581 tgcatcttca tgtattggct tatggtagag agcgaataac cttgttccac cagttcagga
    20641 tagatctcca taagtgtaga ggcaagcttg gatgaccata catggccaat cataacggca
    20701 gcaccctttt gccgggcaat ttgttttcct tcttctattg cagcaagaat tgattcgcgg
    20761 tcgccattgt tatcgagaaa cacatcgcgc tccatatatt gcgttcctat ttttcgactc
    20821 agctccgcac acacagttcc cggagccgta agagaattaa gataatatat gccacgttcc
    20881 ttcgcaagtt tcagaattgg aatcatgcaa ttcgaatcgc gggttacggc ggagcccata
    20941 tgattgttca tgcctgctgg aggatatggg aagctatcga tattctcggc tatggtcttt
    21001 tcgatgattg cttcatccat tgagcaatag acggcatgag gacccggatc attgcctcct
    21061 aaagcctcca tgggttgatg aagtattagc tccttgccag cctcgtgtat tgcaatcaca
    21121 gaatccttgc tgtgtgtaag ttgaggaaga acggccagag taatcggaaa ggggagatgg
    21181 agaaaggcat cgagttctcc gttattatag cctacatcgt cgattacaat gatgagcgta
    21241 ggctgagttg tgctgcccgg caacactgac agcggaccac cagaaccata aggttcgtgc
    21301 tcttcgctct caactttttc aatttcttct tgggcttcag cctgaatatc ccaatccgat
    21361 gctgttctct tggagaccga agttgttcct ttcctttctt caggcggaat aacggccaga
    21421 tcctcgtatc caggaggcag ctcgaataca tattcaggtg ttccataagg tacatcctgg
    21481 ctcttgctaa gtgagtcttt tgatttgata gaattcgata ttattagggc ggcgattact
    21541 aaaagaacag cgctggagag cagcagtaca tagtaagaac ccttatgagt gcgctttttc
    21601 ctcactccct catcgtatct tggacaggct gctttatcaa gcggttaatc ctacctgaag
    21661 tgctgtatgc cttagagact caagagctct cttttctata tgtcgcaccg tttctgccga
    21721 gcatcctaca atagaaccaa cggccttgag cgattgttcg gggccgcacc caaggccaaa
    21781 gcgcttcgaa attacttctt gttctcgttc agaaagagta gatagaagcc aagcgctttg
    21841 ccatgtgtga ctggattttt ctatttcgcg ttcagggtta tatcgccaat cctcgtatac
    21901 atctagaatt gaaagctcat cattgtttgt ctgctcaaga gatacgggtt ggtctcccag
    21961 atttagaatt gcggagatat cggtctcctt ccagcccgtt tctgctgcca gctccttttc
    22021 ggaagggcac ctggataaat cgaggctagc acgcatttgg atagctgata ggcgcctaag
    22081 agcgttttct tttctatgag gcaaacgaat atgtcggcct gtgtttacaa gagaacgcga
    22141 tatagcctgg cgaatccacc agacggcata agtagagaac tttacatttt tcgacggatc
    22201 aaagcgctcg gcagccttta caagccctat attaccttct tgtataagat ccatgatcga
    22261 atgcgggggc gaccacattg atttggcaat gcgcaccacc agacgaaggt ttccttcaac
    22321 aagcctatct aaggcacatt tatctccttt ggcaatcttt gaagccagga tcgtttcttc
    22381 ctgtcgggtt acaaggggaa tttttctaat cgattcaaag tatagctcga ggttttcgtc
    22441 attgtcagca atggtcatga atattcctcc aagactgatt attccataga attagaagca
    22501 aatagtgtgc caaaatggaa aagaaaacga attggcctat atttttatct aggataaagg
    22561 tatttttgaa taaaaacatg aaacatgaaa ctggaaggaa gctcttttag aaataaatgt
    22621 atgatattca ataaccagaa tgcatcttat ctgtataaaa aaatagacac cttgtcttga
    22681 ttctaaatgt tcttatttga catattgtcg tggatccaca gcttgtccat ttctgaagat
    22741 agcaaaatgc aggtgaggtc cggtgctttg acctgtattc ccagatcggc ctatagtttc
    22801 tccttgattt atgcttgttc cttttttgga aagaatggaa tccagatgag catagagtga
    22861 ctgataacca tctgcatgtc ggataatcac ataattgcca aagaccgcat tgtagcctgt
    22921 atctgcaatc ttgccctcgc gggtcgcttt gacggcagtt cctctgttta ctacgatatc
    22981 tatagccgca tgaaaggttc gctgtccgct aaagggattt gttcggtatc cgaacggtga
    23041 agagatctgg ccgcgcacag gccatataaa ggtttcgcca taaaagttgc gcaaaaccga
    23101 tgcatttagt ttggcattcg gtataaaaag gcgctctcct attctaattg ttgcggagga
    23161 tatattgttg gcatcagcta tgcgcgtcat atccactcca taagccctcg aaatgtaaga
    23221 aagactctcg ttctttttga cggtgtgata tacaccattc atattgggaa ttttgagggt
    23281 tttgccaacc tggatctggc ttttggagct cagattgttt gccgagatta ttgtatcttc
    23341 gcgaacacca aatctgcttg cgattgtctg tagagtatca ccacgacgta tagtgtagct
    23401 tttctccgca aggagagtag gaattggagg aaggtccgat ccatcgattg gtgaagagga
    23461 tggttcgggt tcaagcattg ccatgagcgc ttcctgtgcc gagttttcat ctggaagact
    23521 tatattagga tgagaatggg atatgagaga ggctgcaaga acacccgcaa cgcttgtcga
    23581 caatagaaca gcaatcaata aaatagagcg tggcgtcagc tttattagat tctgaaattg
    23641 attgcgggtg gatcgttgta gatcgtcgga ggccttttct ctcgaggaat taagatccga
    23701 gttgacaact gccggagaga aaaggggtcc actagaaggc ctttgcttct ggttcgatga
    23761 ttttgaattc gctcttttgg atctttttgg tggctgcttt cgaatgaagt cttcgccaga
    23821 gagcaccgaa acaagaggct tattgagaat tttatctgct tcgagaacag aattctcaga
    23881 gtgacccaca aatttcatag cttctcttgt atcggcatat tttggtcgat aatttaatct
    23941 aagctgtcga aagccggaat gcaagagatg caagagagca aaagggagat atacatgaag
    24001 agatcgtccc gtccagaagc caaaaagctc gacagcgtaa tgattactat acttgttgtt
    24061 attttcatgg ccttcttggt caggtttttc ctgctcgacg cggccgtagt ggaagggaaa
    24121 tccatgcttc cccattatag gaacggccag gtggtattga tattgaaggc agcttacgga
    24181 atacgaggtc taaatggaaa ttactggatg caatggagac aaccgacaac cggagaagtt
    24241 gtcgctgcta tatcccctgt ctcgcacgag ataatcataa aagaaatagg ggaggttcgg
    24301 agagaaggaa aggagttggc gtatttccta ataggatata atccgattga atcgatagat
    24361 tcaagagatt ttggactcat acgattcgat tcgatcatag gaaaggtcat tcctcagagg
    24421 tagctcggat ggataatagt gcacaagccc gcttccggcg aagaatcctt gtacttgtcg
    24481 ctttgttgac actatttgct ggtgttgtcc tggttcgata tggtctgctg gctctggaag
    24541 ggccagagaa taaagggctc tcggcagagg tgccaattga acgtggaaga attctcgata
    24601 gaaatggaag actcttagct ttcgatattc caaaattcaa tctagccatc cgtaagaatg
    24661 aaatagatcc atatagaatt tccaaggatt tgagtactgt tgcaaagtca atcggcgtgc
    24721 agtctgagtc tcttgaaaag aagataaggg aatcgagcca gaattttata tatctcgcca
    24781 agagactgga catcgatatg gcaaagccca tccaggcaaa aatcgaaaag ggagatcttg
    24841 ctggttttgt gctcgaagaa ataaatggcc gaatctatcc ggaagggaga ctggcctcgc
    24901 acctgatcgg ttttacagga gaagggaata tcggactaga gggcgtagaa ttcaaattaa
    24961 acaaagagct ttcgggcgtt ggaagcgaca aaccacgcgg aaacgatgtc tatctcacta
    25021 tagacgcgcc acttcagtat gcattagaag gaatcgcacg caaggccttt gagcaaaaca
    25081 aagccgaatc catttttttg ttggcaatcg atgtcaatag cggtgaagtc cttgcctatg
    25141 ttcagatgcc agatttcgat ccgaatactt tcggaagttt ccgagaatcg gagcgcgagg
    25201 atagaatgtc tgtgtattca tacgaaccag gaagcgtgtt caagattttt tcgatgtcat
    25261 cgatactcga tgctggtctg ataactccca ggacaagatt caactgcgac ggcgcatatc
    25321 gaaggacgct tccttctggc gaaaaaatcg taataaagga tctgaattcc tatggaatgt
    25381 tggatctggc aggtattctt gccaagagca gcaatgctgg agccggatac gcttcagatc
    25441 gcatctcgga ggacgagttc tattcgcgaa tattgagctt tggttttacg gaaaagacgg
    25501 gaattggatc ccccggtgag aatcccggca acctaaaaga accttcaaag tggtctgcac
    25561 gcacaaaacc cactgtggcg ataggacaag agatacgtgt cacggcactt cagatgatca
    25621 ccgcttcggt tgctatagca aatggaggta ttctgcttaa gccgaacacc atttcccgca
    25681 tcgaggatgc gaatggtaat gaggtttatt cacatgaagt tatagctgtg cggcgagcca
    25741 tagcgcctga gacatccaga gctataattg ccgctatgga atcagccgct agtcttgaag
    25801 gcacaggatg gcgggccaag gtgcccgatg tgcgtatggc tgtaaagact ggaacggcgc
    25861 agatgataga tctaaaaaca aaggcttact cggatacgga ctttatcgcc agcacattgg
    25921 gaatattgcc ggcaaacaat ccaaagcttg caatatatgt tgctattgta aagccaaaag
    25981 gagaatccta tctgggcggc cagattgccg caccggtttt gcgagatgcg gccgaggctg
    26041 ctataagcac ccttggcctg ccgagagaaa aaagtctcaa tgtcaatcat gctggagtcg
    26101 tggtcatcca ggagcctcct tctgcctcga ttggctctat catgccagat ctaaaaggtt
    26161 attcaaagcg tcaattgttg ccccttcttc tgcgaaagga tatcgaagtc cggcttgaag
    26221 gagatgggta tgtagtttct cagtctccat ctccgggtac agccgtagag gctggcacaa
    26281 gaataatgct tcggctacaa tgattcttcg agacatttcc tgcttgaggt atgaaaaatg
    26341 actagagatg aagcagttgc tctatggaag cgatggaata gtgatgcttc tctttggcat
    26401 catgctcttt cggtagaagc ggtgatgcgc cattttgctc agaaatatgg tgagaatgca
    26461 gaatattggg gactcatagg tcttttgcac gatgtcgatt acgagaagtt tcccgaggag
    26521 catttgagac atgcgcggga gatccttaca tcagctggct ttgatgatga attcgttcgt
    26581 tctgtagaaa gtcacggctg gggaatatgc acgcaaatcg cgcctgagct tcgcatggaa
    26641 aaggtgcttt ttgcaattga cgaattgact ggtttcattg ccgcctgcgc ctatgtcagg
    26701 ccatcgcgca gtgtgcttga cttggaagtt aaatctgtca agaaaaaatg gggctctgcg
    26761 gcatttgcgg ctggtgtgcg cagagatgtc atagagcaag gagctgatat gttgggtata
    26821 cctctcgacg agcttattgg tgagacaatt ctagctctta ggagcgttgc ttcggaaatt
    26881 gggcttaagg gcaacctgta gctcaaaggc aattcaataa caaatatata tggaaagaaa
    26941 caatgcttga gaagatttca gagaccttta ccgatgtatt caggacaata agcggcaaat
    27001 ctgcgatctc agagaaaaac gttgaagacg ctctagatcg cattaaaatc gcgctccttg
    27061 aagccgatgt aaatgtgaga attgtgcggc gctttgtcaa tggtgtgtta gaagaagcca
    27121 gaggggaaaa ggtacttcgt tccgtcacac ctggacagca gtttatcaag atagtctatg
    27181 accgaatggt ctctcttctg ggcgatgaaa agcaagacct cctattaaga gggactgata
    27241 ctcagagtgt aatccttctc ttgggtcttc agggttcagg aaagaccact actgcagcta
    27301 agctggctaa ttttcttaaa aaaagaggac gaagagtatt gctcgtggca tgcgatctcg
    27361 tgagacccgc ggctgttgaa cagctagccg tgctcgctgg acaggtcggt gttgatatcc
    27421 atagagagga actaaaagat ccggtacagg tggcacgcaa tgccttggta cgtgcaaagc
    27481 gtgaaggtca tgatgtgctc atagtcgata ctgctggccg tcttcagata gatgaacctc
    27541 tcatgcaaga attagcgaga attcgcgatg ccctttctcc agtagagagt cttcttgtgg
    27601 cggatgctat gactggtcag tccgctgtcg aaattgccaa ggcattcgac gaaagaattg
    27661 gaatcactgg cgccatcttg acgaaattcg attccgacac gcgtggggga gccgctcttt
    27721 cgctaaaatc cattacgggt aaaccaataa agttcattgg tgtcagcgaa cgcatcgatg
    27781 gactcgaacc cttctatcca gataggatcg ccaatcgcat tctaggcatg ggagacatag
    27841 tatcgcttgt ggagaaagcc caggaagtct acgaccaaaa ggaagcgctt gagctcgaac
    27901 aaaaaatcag caaagaaagc tttacactcg aagattattt aggtcaaatt caaaagatga
    27961 agaagatggg ctctgtcaag tcgatgctcg agatgattcc aggtctggcc ggacaagttg
    28021 atgaggaccg catagatctt caggagatga aatatgaaga ggctatcctt ctgtccatga
    28081 caaagaaaga acgccagaat tatctaatta taggtccctc aaggcgaact agaattgcaa
    28141 agggctcagg cacttctgta gctcaagtca ataggctcct taagaaattc gaaaaatcta
    28201 gattgatgat gaaaaaaatg gtaaagaaca aaggagccat ggcgaaaatg tttgggagt
//
LOCUS       DAOOWY010000016        26507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1828_length_26507_cov_11.310600, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000016 DAOOWY010000000
VERSION     DAOOWY010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 26507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26507
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1828_length_26507_cov_11.310600"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PK105_02505"
     CDS             <1..1322
                     /locus_tag="PK105_02505"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOU60610.1"
                     /translation="LKFELSHSLFSSFDIDEGTRFLLKEISNEQGIIHAHRLLDAGCG
                     VGVIGISLAASCPEMDVVLRDRDFRAVALSARNAERNGLGVKLFGLNGQQHVLCQKKK
                     FAKIKIKQRKADDLIAEPGLLCEPDERGPYDAIVSNLPAKAGVNVLENYFHFACTELL
                     QPGGTFAFVIIAPLADMACIWCANAGLALIRTNRTKNHMVAIAQSPREVQVGKLSSNG
                     WISAYLRSHTERQLARANLEWDGFQGLPEFDEPSFVTLCTLSLSQRVFAGLLVRRALV
                     VEPGVGIIPIWIKEVLGTEEIVLISHDVLALTASIHNLEKKGFLKAISILDPILPIIS
                     PSAIENSLPHEALLQEKEGQVSVEKKLSPASIDVAVFFLEDIPRYNIASASWLLTQKS
                     LKRGGVVIFSGTSTHCERVCREKPMGFSRLPHSDQKKGWHAVAFRRD"
     gene            1444..1962
                     /locus_tag="PK105_02510"
     CDS             1444..1962
                     /locus_tag="PK105_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60611.1"
                     /translation="MKKAERMILESRTPDEYVENSLKSRLSPAEKAKLARLWMQGTGF
                     NREDIIHARNRSEYWRKRKMAGTRERTKRRMAQHDYSKGKTIVWTRENIAEFLSLNRK
                     DAYGRYLYRDWELAALFSTSIPSIQYLRRKFNKVSKMLGPGARRDKVIDYMTCSELVL
                     QRGGPKALKSKR"
     gene            complement(1969..4002)
                     /gene="recG"
                     /locus_tag="PK105_02515"
     CDS             complement(1969..4002)
                     /gene="recG"
                     /locus_tag="PK105_02515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOU60612.1"
                     /translation="MTQDISILRGTGPATLRSLANLNIHTIADLLLHLPRDYEDRTKI
                     LPLSRFSEGKVYSKAKVIRHEWFGFGRMRTLKIIIADESSEAALLCFNRPFLEQMAPV
                     GSTIQVYGKFQFKYGEIQSSAFELKTLQDNFVPERTLSPIYPLTGRLNQTTLRRMIAL
                     ALQKFALHLEDELPGTLRELYNFPSKSEAIRSIHFPSSWDSLKSAHDRLAYEELFYFQ
                     LGIALRIQSRQTKTINRIAVPGVLARQLRERLPYTLTEDQELVLKEIIQDMHKPFPMA
                     RLLQGDVGSGKTMVALLAALYAVERGGQVAIMAPTELLARQHATIAAHLVEPLGVRLA
                     FVTGSISSAVRPQLLEALKSGDIDIAIGTHALFSDDVSFKNLELIIIDEQQRFGVLQR
                     IALYKKGHVPDFLMMTATPIPRSLALTFFGDMEVSTIRHLPPGRKPVTTHLANESRRD
                     SVYEFTRKKLKEGQQAYFVYPVISESDRLGLRDAESMAEHLSKKIFPEFKVGILHSRL
                     NDDAKMDVMSRFSEGEISVLVATTVVEVGVDVPNATVMVVEHAERFGLSALHQLRGRI
                     GRGDLQGYCFFVYSKDITEEGRMRLKALYETNDSFAIAEEDLKIRGPGNMFGAEQSGD
                     LQLRIADMRSDFDLLKKARNDAFRIIEDDPSLNSPDNRVIRQVLDRANPYSDK"
     gene            4091..5590
                     /locus_tag="PK105_02520"
     CDS             4091..5590
                     /locus_tag="PK105_02520"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOU60613.1"
                     /translation="MQKETARLIELDREHTLMSHIGAVLGWDQETYMPSKAIEERASQ
                     IALLEGLAHQKAVNPEIDELLATLEEKHDLSLDERAYVRKMRRDYDRETKLPEAFVTE
                     YAKTTSIAQAKWAEAKKNNDFRAFEPHLEKIVDLSRSLAGYLNPNAKPYDVLLDLYEP
                     GNTQQSVASIFSTMKAYLVDILDKIRGRPQIEDRCVGRYVDSETQERISLYFMNVLHY
                     EMDRGRLDVSAHPFTTSLGADDIRITTRYIEDYFPSSLFSTIHESGHALYDMGIDPDP
                     EFRGTKLAEAVSMAVHESQSRLWENIVGRSRVFWERNFLALSALLGKAGEDLDFKSFI
                     ESINRVSPSLIRTEADEVTYGLHIIARFELESALLDGNLRVTDVPGAWREKYKQLLGL
                     EVSNDREGCLQDVHWSIGYFGYFPSYALGNLYAAQFWAKLKEEVPDIEARIASGDVLS
                     VLTWLRANVHCHGSRYMPGELVEKITGSALDPAWFERYLREKYSSIYGF"
     gene            complement(5594..>6651)
                     /gene="hisD"
                     /locus_tag="PK105_02525"
     CDS             complement(5594..>6651)
                     /gene="hisD"
                     /locus_tag="PK105_02525"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOU60614.1"
                     /translation="DSNDSNRPTTLNIATGEMAAKRNTGRGEKGALVGRHGHDLVPVA
                     RVGCYVPGGAFPLPSSAIMTVVPAKVAGVEEVWCAGPRPSNETLAAAWVAGADGFLQV
                     GGAHAIAALAFGICIPRCDIIVGPGGKYVMAAKRQLFGIVGTEAPAGPSELLIIADKT
                     ADPDMIAADLLAQAEHSPDAMPAAIVSEETIALEIEKSLMTRLEDLPEPNQSIAYRAL
                     SNGFLCVEQDLLRIVEGANRCAPEHLEIATSDPVGLMKQIKNAGSIFLGIGSAEVFGD
                     YGAGPNHTLPTGGASRFAAGLSVLCFLRARTWLAIENPELLAEDTAAFARLEGLEAHA
                     QAALARVHKHKNKGPDS"
     assembly_gap    6652..6751
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6752..7206)
                     /locus_tag="PK105_02530"
     CDS             complement(<6752..7206)
                     /locus_tag="PK105_02530"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOU60615.1"
                     /translation="MEKHPLKRIAIDDIAEPSVRLFDPEIEAVAKQAIDAIRAGKEPE
                     LRRWAERLDGLPVGAPLVISRDELKAAYDKLPKKTTELMERAKGRVAAFAAAQRACLT
                     PLELPIRIEPIEMKSQMYSPEARRPPDPNEFSHPGEFIDPSDPNDSSEF"
     gene            complement(7188..8501)
                     /gene="hisE"
                     /locus_tag="PK105_02535"
     CDS             complement(7188..8501)
                     /gene="hisE"
                     /locus_tag="PK105_02535"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOU60616.1"
                     /translation="MIVPSIDITNGRAVQLRGGKQPPLDAGNPLELAERYGRVGEFAV
                     VDLDAAKGVGSNRELILSLCRIYRARVGGGIRTIDLAMDYLNGGARSIIIGTSATPEF
                     LSSLPRERVIAALDSKEGNIMVEGWSKQREGSIEESIIALAPYVSGFLITFIETEGDQ
                     CGIDIERARKLIALAPDSKFTFAGGAAGGERGIADIATLDRLGADLQAGTSLVLGDLS
                     LAEAFAAPLITDRADGLWPTVVCDESGKALGLVHSSKESLKLALNCGKGVYHSRSRGL
                     WVKGESSGNDQILLRAELDCDRDTIRFTVRQNGEGFCHLGRRTCFDNGYGLEKLDRTI
                     KRRLKEAPQGSYTRRLFADEALLASKLREEVQELIQSKSLQETISEAADVLYFALVKT
                     ISMGGGLSDIEEELERRSLRVTRRGGNAKPEFEGLEGKSEWKSIH"
     gene            complement(8498..9265)
                     /gene="hisF"
                     /locus_tag="PK105_02540"
     CDS             complement(8498..9265)
                     /gene="hisF"
                     /locus_tag="PK105_02540"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOU60617.1"
                     /translation="MNGLAVRIIPCLDFKDGRVVKGTRFLNLVDSGDPLERALLYERQ
                     GADELAFLDISATLEGRKTMAQEVARIRQVLSIPIMVGGGIGSIEDACRLLEAGADRI
                     SVNSAAVRNPLIIDELASRFGVQCVVVAIDVAENSTMPSGYEVKIDAGSRKTGFDAVA
                     WAQEASGRGAGEILLTSIDRDGTSLGYECRLIGLVARAVLVPVVASGGASKPEHFLEG
                     YLSGASALLAAGIFHRGEISIREIKEFLKAHDVEVRL"
     gene            complement(9262..9966)
                     /gene="hisH"
                     /locus_tag="PK105_02545"
     CDS             complement(9262..9966)
                     /gene="hisH"
                     /locus_tag="PK105_02545"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOU60618.1"
                     /translation="MSMKERKKLIIIKTGAANLASVEAFAKRLGFEPVVSDMMIDVLE
                     GQVVILPGVGAFGPAMEKLSQDGLDEAICRRVEQGLPTAGICLGMQLFFEASEEAPGS
                     KGLCIFNGTVKRLAGGLPLPQLGWNKIESESSAHLLESGWVYFANSYRVGEVAYTYKT
                     TIKGSKGAPFAQNVFDQVAIASARYGEPFVAALEAWQDAKPTLLLCQFHPELSGSFGT
                     RLFSHWIDLNRIGRNV"
     gene            complement(9963..10565)
                     /gene="hisB"
                     /locus_tag="PK105_02550"
     CDS             complement(9963..10565)
                     /gene="hisB"
                     /locus_tag="PK105_02550"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOU60619.1"
                     /translation="MSDRLMATIKRTTAETDVSVSLQLSPGSIEIATGIGFFDHMLTE
                     LAFHAGWSLQISCTGDLEIDDHHTVEDVGIALGMAFSKIVPSAQGFARFGYSYAPMDE
                     ALARAVLDISNRSYCVFSGTFSMPSIGLLSTCNIAHFFQSFAANARCTLHLDILRGEN
                     DHHKAEALFKAVALAFRAALAPSPVLGSTKGEARIDSELT"
     gene            complement(10588..11613)
                     /locus_tag="PK105_02555"
     CDS             complement(10588..11613)
                     /locus_tag="PK105_02555"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOU60620.1"
                     /translation="MLRLDANEGQCLLSEEDLRAILSPEIARRYPMRELLEIPLAEKL
                     GIPSSCLLATAGADDAIDRAIRSLAGPGGLVMSTTPGFVEFLEAAQRSSARFEEIAKN
                     PFGPFPIDDICRCVKDFAPKLLILSSPDNPSGVVLSPLDVTQIAKTCLEAGTIFVFDA
                     TYQEFSIHGATPIQALANPNVLVTGSFSKSKGLAGFRIGYAAATPESARIISKLSDAG
                     PPYSLSSSAIEAGRRALSLDQGVAQGFIDEVRREVHVLKSLCRKLGFLVSESEANFVL
                     LKIKNPIDLSDRLRERGILVRTWPNKPGYEELVRITVPGEPEEFKELCVSLEAAIKEY
                     NENYRMK"
     gene            complement(11631..12572)
                     /gene="hisG"
                     /locus_tag="PK105_02560"
     CDS             complement(11631..12572)
                     /gene="hisG"
                     /locus_tag="PK105_02560"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOU60621.1"
                     /translation="MAENANQKLELRLGLPKGRMQAKVIELFKDTGIPISIDDREYRP
                     RIGNGKALVFGSREIMYEAKLLKPQNIVEMLEAGSRDIGFAGEDWVRELGADLVELLD
                     TGLDPVKIVAAAPENVATAFRAYRGDLTKMISVCKRTPIIASEYEGLARAWIDKNLPG
                     AGFVHSYGATEVFPPEDADIIIDNCATGSTLRANRLEILDTLMMSSTRFYASKAAMAD
                     ATKREAAEALILLFGSVLEARRRVMLEVNVSAELLDELVAILPCLRAPTVSPLGGGDA
                     FAVKVAAPRDTLASLIPEIKRRGGTDIVATQISQLVP"
     gene            complement(12931..13527)
                     /locus_tag="PK105_02565"
     CDS             complement(12931..13527)
                     /locus_tag="PK105_02565"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOU60622.1"
                     /translation="MPELEKYDHGPTKIERNNVIERLEHGFAQNLLDIDVYEKRMEIA
                     VNTASKRELLSLIEDLPASTETNKQFTSLDGILLNHGEIKDYDVTLAFLSGATRKGLW
                     YPPKRMKVVAIMGGVDLDYSQAQIPPGVSTIDVFALMGGVDIFVPEGVNVEVTGIPIM
                     GGIDNRAPSSAKLGAPTIKINAITLMGGLDIKVKKPKN"
     gene            13725..14705
                     /locus_tag="PK105_02570"
     CDS             13725..14705
                     /locus_tag="PK105_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60623.1"
                     /translation="MSNLCQEKDGNLHDFELHPRGRGGAAIIYPVISRRAGGLSLGIN
                     LFPERKICSYDCPYCEVMPFSNPGASLRAGDVSRAIECFFDEEWPELSKTLELKDISI
                     SGNGEPTLSPFLEEALYAARQAIDTKALVKDCATPSGQHSIPSIVLITNSTGFLREDI
                     TELLAGFYKRYPFEIWAKLDGGKEELHALLSRSRFRFAEIVRGIVDFSCSVPVTLQTM
                     VIYDNRTGNIYFDCQSYAVTIREILNGGGQLRAIQIYTTARVPVESWVAPVSDERLVI
                     FARQLSSILNRPQRKKATGKEEEALQNQVYSIPILCYGSRGKLDWNHANR"
     gene            14692..15543
                     /locus_tag="PK105_02575"
     CDS             14692..15543
                     /locus_tag="PK105_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU60624.1"
                     /translation="MPTADLHTHSTASDGELAPAALVEKARALGLSTLALTDHDNTLG
                     VAEARAAARLVNLEFIPGIEIEIDFEPGEFHLLGYGIDEAAPPLVEAVERLSHARLER
                     NLAILDRIAKANLPLDLDSIEAFSKTSYLGRPHLADLFVEAHCARTRQEAFDRYLGKG
                     KPFYIKKACLALEEALSVIDAAGGVSVLAHPYSLFVSKAVLGTLFSQLKAKGVRGIEA
                     YHPTAKYGQCVILERMAHERGMFVTAGSDFHGKTRPECGLGRTSGNEPIDIRFRDELV
                     AALESRI"
     gene            complement(15677..16777)
                     /locus_tag="PK105_02580"
     CDS             complement(15677..16777)
                     /locus_tag="PK105_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU60625.1"
                     /translation="MKTTNPRKISFLILFLALLLLVGRLFYPFLTVILWSGLLYVFLE
                     PIHKRLRGPNSIKGRKTMRSRLSALFLSIFGVIIILIPLAFLAFKTIQQLADLLKLSI
                     HFVEENIDKLRIDPQNQIGSAIQSLLGDLYDVSTFDPLRELQSFLASGVNQLIKISTS
                     LIMNISKFVVTICFMVFTLYYLLMDASSLGDTFVSMMPIAPQYTRLFMSKLRETGRQL
                     VIGYFLVSLFQGFMMFILSIIFGFKNNLVLAVLTAISSFVPLLGTTVVWAPMGIYLAV
                     NGQIIKAIVFVACASVIVATLDNFIRPIVLGEQIKIHPLLLFFSITGGLAVFGFNGLI
                     LGPVILILFVAAGDLYRALYEESELSDNKNEK"
     gene            16973..17665
                     /gene="rsmI"
                     /locus_tag="PK105_02585"
     CDS             16973..17665
                     /gene="rsmI"
                     /locus_tag="PK105_02585"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOU60626.1"
                     /translation="MIATPIGNLEDISLRALETLKSVDIIACEDTRHTLKLLSRYEIR
                     KPLISCHSYNEQQAAKRVIALLSEGKDVAYCSDAGTPGFSDPGALLVRMARSAGYKIV
                     PIPGPSAFACLISASGLSGKTFLFDGFLSPKPGKRRTRLKELLDRGESFVLYESPFRI
                     GKLLADIASIDPERRLCIGRELTKIHEEIIEGKAEELVQRFPAESAKGEFCIIVHGIK
                     PATSVNDKEDES"
     gene            17662..18477
                     /locus_tag="PK105_02590"
     CDS             17662..18477
                     /locus_tag="PK105_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOU60627.1"
                     /translation="MMDISHFRNLHGRARLRKAVLLLERLEREWRKADPLIIPYEDLV
                     FVKQIAKILADSEETTLECVKAASDFLGSLSGLDDILDYYGFLQTENKFRHRLMILSG
                     QGVADWDFIAVPRSSAGHATKRAGMRVYLEDLRSPFNVGSIFRTAEALGFEEIIISPE
                     CADPMHPRAIRSAMGTIERMKWRRAELSELDCIQGVFALELGGIAIDKFSFPFPGLMV
                     VGSEELGVSNKALQYCRLGCIEIPLVGTKASLNVATAFGIAAYSWYYSGNMQA"
     gene            18545..19435
                     /locus_tag="PK105_02595"
     CDS             18545..19435
                     /locus_tag="PK105_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU60628.1"
                     /translation="MSLNPFMKKLGLSSSDRAVIVHVDDVGMCLSSIEAFFELYDFGT
                     VTSGALMAPCPWFPEVARRCAARPDVDVGVHATLTSEWKGYRWGPVSTRNPDSGLLDA
                     EGYFPMTSEEVQSGAKPEAAMKEIAAQVAMVYAAGIEPTHIDTHMFSVAHEKLFDAYI
                     SQALERRIPAMLPKPDIVGFESIVPDCETRKYLAGYLKELGEKGMPLMDGILAMPLDE
                     PRDQTETVKRLLGSITEGITHFVLHASTDSHELRAITPDWESRVWNYRTMHSDTMKKI
                     IESEGIVLIGYRNLRDAMRK"
     gene            19572..20693
                     /gene="ugpC"
                     /locus_tag="PK105_02600"
     CDS             19572..20693
                     /gene="ugpC"
                     /locus_tag="PK105_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOU60629.1"
                     /translation="MAKVELKNIGKVYEGNVRAVSDANINIHDKEFVVFVGPSGCGKT
                     TTLRMIAGLEDITEGEIYIDGRLVNDVPPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     IRKLPKEEIDNRVKEAARILDIEKLLDRKPKQLSGGQRQRVAVGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRAELIELHDRLQSTMIYVTHDQVEAMTMGDKIVVMKDGLIQQIGS
                     PLYLYNNPINKFVAGFIGSPPMNFMSVNVIEEGGQVLIDEGSFKLAPDAKHAELLKAY
                     VGKSVYFGIRPEDLPVADAVHRGQKFNAKVTVVEPLGSEIHLQATTPTQGMVSRIPPH
                     HLYKHGDTIIFAPVMAKAIYFDKETEKSILPVKWNEQEA"
     gene            complement(20969..22267)
                     /locus_tag="PK105_02605"
     CDS             complement(20969..22267)
                     /locus_tag="PK105_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU60630.1"
                     /translation="MSEKKGFKPYLGITFLIGFGFFTMGLMDPLYDTYVPIFLSRYLS
                     KMSLVGFFMTLDNVLAIFLIPLVSAWSDNTYTKLGRRMPYILVLLPLTAFLFGAIPYA
                     TEVSLVALLVTLILLNITKQSVRGPVVALMPDTIPADYRSEANGVINTMGGIASIVGT
                     IGLARLMDLDITLPILGPTKDKLPFPLGGLFVILAVILLFIFIREKQPDAKETQEQKV
                     PILESFRNVAAQKDKSALYILIAIFLWFLGYQGVLPFIGKYSVDVLRTSSGMAALAAG
                     MVGIAYAIFAIPSGYVAHRFGRKKTIRTCLLVISILTLLLFFHKWITAGLSNSLRLGS
                     FWAIMFLFGIFWVSIITNSFPMLWQMAEWGTIGIYTGLYYTASQAAAILGPVLTGVII
                     DIFGYQGIFLFCTICMICARAIMGKVTKGEPSEIQNATQK"
     gene            complement(22300..23556)
                     /locus_tag="PK105_02610"
     CDS             complement(22300..23556)
                     /locus_tag="PK105_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOU60631.1"
                     /translation="MIQQKIPSIPLASFQDLEKEGRRARYDSLVSRLPLGEDSHSRAL
                     HYYSSPGRTELGGNHTDHNNGCVLAASVDLDMLALARPRSDSMVTLLSEGYSPIRVDL
                     RDTAPRIEEKGSPASIIRGLAAWLTHQGLQIGPGFDIAMDSRVPAGSGLSSSAAFELL
                     IASIFDDMGKYDLTPTDWAIAGQWAENEYFGKPCGLMDQIACAEGGIVFIDFADSRRP
                     KIDRLIYDFESNGLILAIVNTGSNHEDLTREYADIPKEMKCVAAILGRTALRGTEKQD
                     LLNRLSDIRIKCGDRALLRAWHFVYENYRPARMVEALKRNDKSAYLSIVNESGRSSWH
                     YLQNIHAGNPREQSLSIALMLSEDLLSPEGAWRVHGGGFAGTIQVYVPQSRFPEFVER
                     MEAVFGKGSVQRINIRPFGVCKVLMN"
     gene            23883..25184
                     /locus_tag="PK105_02615"
     CDS             23883..25184
                     /locus_tag="PK105_02615"
                     /inference="COORDINATES: protein motif:HMM:NF006388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU60632.1"
                     /translation="MNQLAEELNAILAGTVAERLLSEMGRRMYFPRGIITQSAEAGEK
                     AYRFNATIGMAYEHGQPMMLDALRAELPGLTPTEAVAYASTGGIAELRKVWKESLYRK
                     NPSLDAKKISLPAVVPGLTAAVSYICDMFVEPGDTVILPDLHWPNYRLIVEERKSAAG
                     ITYPLFAGKGYNIEGLLEGVRQAGQRRGKAIIILNFPNNPTGYSLTLKEADSITAGLL
                     EIAKQGTDILVIADDAYFGLQYEDGLLCESMFARLADIHPNILAIKADGPTKEDYVWG
                     FRVGFVTFGAAGFGDEHYEALAKKLLGVIRSSVSSSSGLSQNLLLKALKYQGYEAQKA
                     RYRDILKGRYKAMKNKLAEMTLPDGLIPMPFNSGYFMSFRCDGFSAEALRKKLLNEKG
                     IGTVSLMDRYLRVAFSSVEESDISDLCDAIAQAVVELCQQG"
     gene            complement(25214..26431)
                     /locus_tag="PK105_02620"
     CDS             complement(25214..26431)
                     /locus_tag="PK105_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOU60633.1"
                     /translation="MKNAYSFPLENFINRQKFERIKEFSRDKETPFLIIDLDIIKKNF
                     EDLRAYLPWATIYYAVKANPDDAVISLLRDLGSNFDVASRYELDQLLSLGISPDRMSF
                     GNTIKKEKDIAYFYEKGVRLFVTDSINDVDKLSRAAPGSKVFFRLLTEGLGADWPLSK
                     KFGSHPDLARQLIKTAIRFGLEPYGISFHPGSQQRDVGQWSSALTIAAQLFNWARHDL
                     KIDLKMINMGGGFPANYLDPTDPLQQYAEDIKRFLDNSFGLVWPEKIIIEPGRSMAGD
                     AGVIVTEIVNIAKKSVHDRYPWVFLDIGKFGGLIETLGESIKYPIYFEGQGSAEEIIL
                     AGPTCDSMDILYERTPYFMPSSAKVGDKVYILTTGAYTRSYSSIYFNGFPPLKSYVLQ
                     SAQDQSSMTAKEI"
BASE COUNT         6992 a         6114 c         6251 g         7050 t
ORIGIN      
        1 agctcaaatt cgagctatct cattcgcttt tttcttcttt tgatatcgat gaaggaaccc
       61 gctttcttct aaaagaaata tctaatgagc aaggaattat tcatgctcat cgccttctcg
      121 atgcgggctg cggggtgggt gtaattggca taagcctagc agcctcatgc cctgagatgg
      181 atgtagtatt gcgtgaccga gatttccgag cagtcgcatt atctgcgcga aatgcagaac
      241 ggaatggtct tggtgtaaag ctcttcggac tgaatggaca gcagcatgtc ctatgccaga
      301 agaaaaagtt tgcaaagata aaaataaagc agaggaaagc ggatgatctc attgccgaac
      361 cgggtttgct atgcgagccg gatgagcgag gtccctatga tgcaattgta tcaaatctgc
      421 cggcaaaagc tggagttaat gtactcgaga attattttca ttttgcatgc accgagctct
      481 tgcagcccgg aggcactttt gcctttgtca tcatagcacc tttggcagat atggcttgta
      541 tttggtgcgc caatgcgggt ctcgctctaa taagaaccaa cagaacaaag aatcatatgg
      601 ttgcaattgc gcaaagccca agagaagttc aagtaggtaa gctctcatcg aatggctgga
      661 tttcagccta tttgcgttcg catacagaaa gacagcttgc tagagcaaat ctagagtggg
      721 atggttttca aggcttgccc gaattcgatg agccatcttt tgttactcta tgtactctct
      781 cattatctca gagagtcttc gcaggtcttc tcgtgcgtcg ggcgcttgta gtggagcctg
      841 gcgtaggtat catacctata tggataaaag aagtcttggg aacagaggag attgtactga
      901 tatcgcatga tgtgctcgcg cttaccgcat cgatccataa cctagaaaag aagggttttc
      961 ttaaagctat ttccatactc gatccaatcc ttccgattat ttcgccttca gccatcgaga
     1021 attcattgcc acatgaggct ctattacaag aaaaagaagg acaagtttcg gtagaaaaaa
     1081 agctcagccc agccagcatt gatgtagctg ttttcttcct cgaagatatt ccaaggtata
     1141 atatcgccag tgcttcctgg ttacttaccc aaaaatcact aaagcgaggg ggagtagtta
     1201 tattttctgg aactagcaca cattgtgagc gagtctgccg agaaaaacct atgggctttt
     1261 ccaggcttcc acattctgat cagaaaaaag gttggcatgc cgtagccttt aggcgagatt
     1321 aggtagataa ggcttgcaaa ctccatcagg tttttagaca atgagatatc tagagatggg
     1381 gatatatctt tctgcgaact taggatattt gacttttagc aattctttat tagattttac
     1441 aatatgaaaa aagctgagcg catgattcta gaatctagaa cacccgatga atatgtggag
     1501 aattctctca aatcgcgact ttctcctgca gagaaagcta aactagctcg gttatggatg
     1561 cagggaacag gctttaatag agaggatatc atacatgctc gcaatagaag cgaatactgg
     1621 cgaaagcgta agatggctgg tacaagagag cgaacaaaaa ggcgaatggc tcaacatgat
     1681 tactcaaagg gcaagaccat cgtatggacc agagaaaata ttgcagaatt tctatcgctt
     1741 aatcgcaagg atgcgtatgg acgctatcta tatcgcgatt gggaactagc tgctcttttt
     1801 agcactagca taccctcgat tcaataccta agacgcaaat tcaacaaagt cagcaaaatg
     1861 ctcggtcctg gtgcaaggcg agataaagtc atcgattata tgacctgttc cgagctagtg
     1921 cttcaacgcg gaggcccaaa agccctcaaa agcaagcgct aataacctct atttgtcaga
     1981 ataagggttt gcgcgatcta gaacttggcg aattactcta ttatcggggc tgttgagcga
     2041 tggatcatcc tcgatgattc taaaagcatc atttcttgct ttcttgagaa gatcaaaatc
     2101 gctgcgcata tcagcaatgc gcaattgcag atctcctgat tgctctgcgc cgaacatatt
     2161 tcccggtcct cttatcttca agtcctcttc cgcaatagca aagctatcgt tggtctcata
     2221 gagagctttc aacctcattc gcccttcttc cgtaatatct tttgaataga caaaaaaaca
     2281 atagccctga agatcacctc ttccaatacg tcccctcaac tgatgcagag ccgacagtcc
     2341 gaaacgctcc gcatgctcga caaccattac cgtcgcatta ggcacatcga ctccgacttc
     2401 gacgacagtg gttgcgacca agacagatat ttcaccttca gagaaccttg acatcacatc
     2461 catctttgca tcgtcgttca agcgagaatg caaaattcca accttgaact caggaaagat
     2521 cttcttggaa aggtgttctg ccatcgattc cgcatctcta aggccaagtc tatccgattc
     2581 actgataacc ggatatacga aataggcttg ttggccttct ttgagttttt tgcgtgtaaa
     2641 ctcgtatacg gaatctcttc gggattcgtt cgccagatgg gtggtgactg gcttgcggcc
     2701 aggtggtagg tgccgaatcg tagatacttc catgtcacca aaaaaggtaa gagcaagact
     2761 ccttggtatg ggggtggcgg tcatcatgag aaaatctggc acatggcctt tcttgtagag
     2821 agctattctt tggagcactc caaagcgctg ttgttcatct atgatgatca gctcgagatt
     2881 cttgaaagaa acatcatcgg agaagagagc atgtgtgcct atagcgatat ctatatcacc
     2941 acttttcaga gcttctagta gttgaggccg aactgcgctc gatatcgagc cagttacaaa
     3001 tgcaaggcgt acacccaaag gctcgacaag atgcgcggct atggttgcat gttggcgtgc
     3061 gaggagttca gtaggagcca tgattgccac ctgtcctcct cgctcgactg catatagtgc
     3121 tgcaagcaga gccaccattg tctttcctga tccgacatct ccctgcagaa gtctggccat
     3181 aggaaaaggt ttgtgcatgt cctggattat ttctttgagg accaattctt gatcctcagt
     3241 aagcgtatac ggaagacgtt cgcgaagctg tcttgcaagt acgccgggaa cagcgatgcg
     3301 atttatggtc tttgtctgtc tggattgaat cctaagagca attccaagct gaaagtaaaa
     3361 gagctcttcg taggcgagcc tatcatgagc agacttgagc gaatcccatg atgaaggaaa
     3421 atgaattgag cggatggctt cggacttgga tggaaagttg tagagttcac gcagagttcc
     3481 tggaagttca tcttcaagat gaagcgcgaa tttttgaagt gctagggcta tcatccttct
     3541 aagagttgtc tgatttagcc ttccagtgag cggataaata ggcgatagag tccgttctgg
     3601 gacaaagtta tcctgtaggg tttttagctc aaaggcagaa gactggattt ccccatactt
     3661 aaactggaat ttgccataga cttggattgt agaaccaaca ggggccatct gctcgagaaa
     3721 aggcctgttg aagcagagaa gggcggcctc ggaagattca tccgcaatta tgatcttcag
     3781 tgtgcgcatc cttccaaaac caaaccattc atgacgaatg accttagcct tagagtagac
     3841 cttgccttca gaaaaacgcg ataaaggaag gatttttgtt ctgtcttcgt aatctctcgg
     3901 cagatggagc aaaagatccg caatagtatg aatattcaga ttagcaagtg aacgcaaggt
     3961 tgcaggtcct gtacctctga gaatgctgat atcttgtgtc aattctctta aaaacactgg
     4021 ttttagtata caaagagtct tattccagtg ctactatatg catgatgaaa agtgataaag
     4081 gaggaaatcg atgcaaaaag agacagctcg ccttatagag cttgatcgag aacacacgct
     4141 tatgtctcat atcggcgctg tccttggatg ggatcaggag acctatatgc cttccaaagc
     4201 gatcgaggaa cgtgcctctc agattgcact tctcgaaggg cttgctcacc aaaaagcggt
     4261 aaatccagag atagatgagc tgcttgcgac gctagaagag aaacatgatc tttctctaga
     4321 cgagagagca tatgtccgca agatgcggcg agattatgac agagaaacga agctgcctga
     4381 ggcatttgtc accgaatacg ctaaaaccac tagcattgca caagcaaaat gggctgaagc
     4441 gaagaagaac aacgatttta gggcattcga gcctcacctt gagaaaattg tggatctttc
     4501 gcggagttta gcaggatatc taaatccgaa tgcaaagccc tacgatgtgc tcctcgacct
     4561 atatgagccc ggaaatacgc agcaatcggt tgcatctatt ttttctacaa tgaaggctta
     4621 cctggtcgat attctcgaca agattcgcgg cagacctcaa atagaagaca ggtgtgtggg
     4681 gcggtatgta gattctgaga ctcaagagcg aataagctta tatttcatga atgttctgca
     4741 ttacgagatg gatcgcggaa ggctggatgt ctctgcccat ccttttacca cgagcctcgg
     4801 tgcagatgat atacgcatta cgacgcgcta tattgaagac tattttcctt caagtctttt
     4861 tagcacgatc catgaatcag gtcatgcgct ttacgatatg ggtattgatc ccgatcctga
     4921 gtttaggggg acaaaactgg ctgaagctgt atcgatggca gttcatgagt cgcaatccag
     4981 gctgtgggaa aatatcgttg gtcgatccag ggtattttgg gagaggaatt tcttagcttt
     5041 gagtgctctt ctgggtaagg ctggtgagga tcttgacttt aagagcttta ttgaatcgat
     5101 caatagagtg agtccctcgc ttatcagaac cgaagcagat gaagtcacat atgggttgca
     5161 cataattgcg cgttttgagc tagagtcggc gctattagat gggaatctgc gtgttacgga
     5221 tgtgcctgga gcatggagag agaagtacaa gcaactgctg ggtcttgagg tatcaaatga
     5281 tcgagagggc tgtcttcagg atgtgcattg gtcaatagga tatttcggat atttcccgag
     5341 ctatgcactt gggaatctct atgccgcaca gttctgggca aaactcaagg aagaagtacc
     5401 tgatatagaa gctcgaatag caagtggtga tgttctctcc gtgctaacat ggcttcgggc
     5461 taatgtgcat tgtcatggtt cacgctatat gccaggtgaa ctcgtcgaaa aaatcactgg
     5521 atcggctctc gatccagcgt ggtttgagag atatttgcga gaaaaatact cgagcatata
     5581 tgggttctga gttttaggag tcgggacctt tatttttgtg tttgtgcacg cgcgcaagtg
     5641 cggcttgtgc gtgtgcctcg aggccttcca ggcgggcaaa agcagcggta tcctctgcga
     5701 gaagctccgg attttcgatg gcaagccatg tcctggctcg caagaagcaa aggaccgaca
     5761 gaccagctgc aaatctcgat gctcctcctg ttggcagagt gtggttgggt cctgcgccat
     5821 agtctccgaa tacttcggct gatcctatgc ccagaaagat agaacccgcg ttctttatct
     5881 gtttcataag gcccacagga tccgaagttg cgatctcaag gtgttcgggc gcacagcgat
     5941 ttgctccttc gacaattcgc aagagatctt gttccacaca aagaaagcca ttcgaaaggg
     6001 ctcgataagc tatgctttga tttggttcag gaagatcttc gagtctagtc atgagagatt
     6061 tttcaatttc aagggctata gtctcttcgc ttacaatggc tgcgggcatt gcatctgggg
     6121 agtgctctgc ctgggctaga agatctgcgg cgatcatgtc ggggtctgca gtcttatcgg
     6181 ctataatgag gagttcggag gggcctgccg gtgcttcagt gcccacaatg ccaaagagtt
     6241 ggcgttttgc tgccataaca tatttcccgc caggccccac gatgatgtcg caccttggaa
     6301 tacatatgcc aaaagccagg gcagcaatag catgggcgcc tcctacctga aggaatccat
     6361 cagcacccgc cacccaggcg gcggctaagg tttcattgct aggtcttggt ccagcgcacc
     6421 agacttcttc gacgcctgca acttttgccg gaacaacagt catgattgcg ctcgagggca
     6481 gtggaaaggc tccgcccgga acatagcagc ctacgcgcgc cacgggcacg agatcgtgcc
     6541 cgtggcggcc gaccagcgcg cctttttcgc ctctacctgt atttcttttt gctgccattt
     6601 cccctgttgc aatatttagc gttgtgggcc ggtttgaatc gtttgaatct tnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctaaactcg cttgaatcgt ttggatcact
     6781 tggatcgata aactcgcctg gatgactaaa ctcgtttgga tctgggggcc ttctggcctc
     6841 tggcgaatac atctgagatt tcatctctat cggctctatt cgtatcggca gctctagtgg
     6901 tgtaagacag gcgcgctggg cggccgcaaa ggccgctacg cggcctttgg cgcgctccat
     6961 tagctcggtg gttttcttag gcaacttatc ataagcagcc ttcaactcat cccgggagat
     7021 tacaagaggc gccccgactg gaagcccatc taatctctct gcccatcgcc tgagttctgg
     7081 ctccttgcca gctcggatag catcgatagc ttgcttagca acggcttcaa tttcaggatc
     7141 gaaaagcctc accgaaggtt ctgcaatatc atcaatcgca attcttttta atggatgctt
     7201 ttccattcgc tttttccttc aagcccttcg aattctggct ttgcatttcc cccacgccgc
     7261 gtaacacgca aggaacgacg ctcgagttct tcctcaatat ccgaaagacc gccgcccatg
     7321 ctaattgtct ttaccaaggc aaaatagagt acatcagcag cctcagatat cgtctcttgt
     7381 agagatttag actgtataag ctcttgcact tcctcgcgaa gcttggatgc gagaagcgct
     7441 tcatcggcaa atagcctgcg cgtataagag ccctggggtg cttctttcaa gcgtctcttg
     7501 atagtacgat cgagtttttc cagaccataa ccgttatcaa agcaggttcg tctgccaaga
     7561 tggcagaagc cttcgccatt ctgacgcacc gtaaagcgga ttgtatctct atcgcaatcg
     7621 agctctgcgc gcagaaggat ttggtcgttt ccggatgatt ctccttttac ccagaggccc
     7681 ctcgatctag aatgatatac tcccttgccg caattcaaag caagttttag actctccttg
     7741 ctcgaatgca caagcccaag tgcttttccc gactcgtcgc atacaacagt aggccatagt
     7801 ccatcagctc tgtcagtaat aaggggtgca gcaaatgcct cagcaagaga aaggtctcct
     7861 aaaacaagcg aagtacccgc ctggaggtct gcacccagcc tatccagagt cgcaatatcc
     7921 gcaatgcctc gttcgccacc agcggcccct ccggcaaatg tgaatttgct atcaggggca
     7981 agagcgatta gcttccttgc tctttcgata tcgataccac actggtcgcc ctcggtttct
     8041 ataaaggtaa tgagaaagcc ggacacatag ggtgcaagag ctatgatcga ttcctcgata
     8101 gaaccttccc tttgctttga ccatccttcc accatgatat tcccttcttt actatccagg
     8161 gctgctataa cccgctcccg aggcaagctg gaaaggaatt caggtgtggc cgaagttcca
     8221 ataataatcg agcgggctcc tccattgaga taatccatag caagatcaat cgtgcgaatg
     8281 cctccaccta cgcgcgctcg atatattctg cacaaagaaa gaatgagttc tcggttgctt
     8341 ccaactcctt ttgccgcgtc gaggtctact acggcgaact ctccaacccg accatagcgt
     8401 tcggcgagct ccaaagggtt tccagcatcc aaaggtggct gctttcctcc tctaagctgt
     8461 actgctcggc cattcgttat gtcgatactg ggcacgatca taatcttacc tccacatcat
     8521 gtgctttcaa gaattccttt atctctcgaa ttgagatttc tccgcgatga aaaatgcccg
     8581 cagccaggag cgctgatgct ccacttaaat atccctccag aaaatgctcc ggctttgaag
     8641 cccctcccga ggcgacaact ggcacgagca ctgcccgcgc gacaagtcct atgagcctgc
     8701 actcatatcc aaggcttgta ccatctcgat ctatcgaggt tagaagaatc tcaccggcac
     8761 ctcgaccgct agcctcctga gcccaggcga ctgcatcgaa accggttttg cgagagcctg
     8821 catcgatttt cacttcatat cctgaaggca ttgtagaatt ctctgccaca tcgatagcca
     8881 caaccacaca ctgtacacca aagcgcgaag caagttcatc gataatcaag ggattgcgca
     8941 ccgctgcgct gttcacagag atacgatccg ctcctgcttc aagcaggcgg catgcatcct
     9001 cgatgcttcc gattccacct cccaccatga ttggaatgga gagcacttgc cgtatacgtg
     9061 ccacttcttg agccatggtc tttctgccct ccagagtggc gctaatatcg agaaaggcga
     9121 gctcgtccgc tccttgcctt tcgtacaaaa gagcgcgttc aagcgggtcc cctgaatcga
     9181 caaggtttag aaatcttgtt cctttgacta cgcgtccatc cttgaagtcc aggcaaggga
     9241 ttattctcac tgcaagtccg ttcatacatt tctcccaatt ctgtttagat cgatccagtg
     9301 cgaaaaaagt cttgtaccaa aggacccgga aagttccgga tgaaactgac agagaaggag
     9361 tgtgggtttt gcatcttgcc aggcttccag cgccgcaaca aatggctctc catatcttgc
     9421 actcgcaatg gctacttgat caaaaacatt ttgcgcaaag ggggcacctt tagagccttt
     9481 tatcgttgtt ttataagtgt aggccacttc tcccacccgg taactattag caaaatagac
     9541 ccagcctgat tcgagaagat gcgcagagct ctcagattca attttgttcc accctagctg
     9601 aggcaagggc agacctcctg ccagcctttt tacggtacca ttgaaaatac aaagcccctt
     9661 cgagcccgga gcttcttcgc tcgcttcgaa aaagagttgc ataccaagac atatacccgc
     9721 cgttggaaga ccttgttcta cacgtctaca tatcgcctcg tcgagaccat cctgggatag
     9781 tttctccata gctggtccga aagctcctac tcccggaagg ataacaactt gtccctcaag
     9841 tacatcgatc atcatatcgc tcactacagg ctcgaaacct agccgttttg caaatgcttc
     9901 tacggaggca agatttgctg ctcctgtctt gataattata agtttttttc tttccttcat
     9961 gctcatgtta gctccgaatc tatgcgtgct tctccttttg tgcttcctaa tactgggctt
    10021 ggtgctagag cagctctgaa cgccagagca acggccttaa aaagcgcctc agccttatgg
    10081 tggtcgtttt ctccgcgaag aatgtcgagg tgaagtgtac atcgtgcatt cgccgcaaaa
    10141 ctctgaaaga agtgggcaat attacaggtc gaaagcaagc ctatagatgg catagaaaac
    10201 gttccggaga agacacagta gcttctgtta gaaatatcca atacagcacg cgccagagcc
    10261 tcatccattg gagcatagga atatccaaag cgtgcaaagc cctgtgccga aggaactatt
    10321 ttcgaaaacg ccatgcccag agctatgcct acatcctcta ccgtgtgatg gtcatcgatt
    10381 tccaggtctc ctgtgcatga aatctgcaag ctccagccag catggaatgc aagttcagta
    10441 agcatatggt caaaaaatcc tatgcctgtt gcgatttcga tggatcctgg cgataattgc
    10501 agactcacgc tgacatctgt ctcggccgtt gttcttttta tagtggccat tagtcgatcg
    10561 ctcatagacg ctccttaatc caaggtatta tttcatgcgg taattttcat tatattcttt
    10621 tatagctgct tcgagagaaa cacacagctc cttgaattct tcaggttctc ccggaacggt
    10681 gattctaaca agctcttcat aacctggctt attaggccat gtacgtacga gaattcctcg
    10741 ttcgcgtaac ctatctgaga gatcaatagg atttttgatc ttcaacagaa cgaaattagc
    10801 ttcgctctcc gataccaaaa agcctagttt tctgcaaagc gatttcaaaa catgtacctc
    10861 ccgccgaact tcatcgataa agccttgagc aactccttga tcaagagaaa gcgctcttct
    10921 gcctgcttcg atcgctgagc tcgaaagcga ataaggagga ccagcatccg aaagtttcga
    10981 gataatcctt gctgattcgg gagttgccgc agcataacct atgcgaaagc ctgcaaggcc
    11041 cttggatttc gaaaagctac cagttacgag cacatttggg ttggctaaag cctgaatagg
    11101 cgttgctcca tgtatcgaaa actcctgata ggtcgcatcg aaaacgaaga ttgttcctgc
    11161 ctcgagacag gtcttggcta tttgtgtcac atctagggga gacagcacaa ctcctgaagg
    11221 gttatcgggg ctcgagagta tgagcagttt tggagcgaag tcttttacac acctgcatat
    11281 atcgtcgatc ggaaaaggtc cgaatggatt ttttgcaatt tcctcgaatc tagcggatga
    11341 tcgctgggca gcttccagga attcgacgaa accgggagtg gtgctcatta caaggcctcc
    11401 tggccctgca aggctccgta ttgctcgatc gattgcatcg tcggctccgg ctgtggcgag
    11461 caggcaggat gaaggtatac cgagtttctc ggctaagggt atttccagaa gttccctcat
    11521 agggtagcgt cgggctatct ctgggctcag aatcgctctg agatcttctt ctgagagaag
    11581 gcattgacct tcgttagcat ctaatctaag cattttcttc tccttttgcc ttagggcaca
    11641 agctgcgata tctgagtggc tacaatatcg gtgcctccac gtcgctttat ctctgggata
    11701 aggcttgcga gtgtatcgcg aggagccgca actttaaccg caaaggcatc tccccctccg
    11761 agaggggaca cagtgggtgc tcgtagacaa ggaagaatgg cgacaagttc atcgagaagt
    11821 tctgctgata cattcacttc gagcattaca cgacgcctcg cttcgagcac agaaccgaag
    11881 agaagaataa gcgcttccgc tgcttcgcgc ttggttgcat ctgccattgc tgcctttgag
    11941 gcatagaatc ttgttgaact catcatcagg gtatccagta tttcgagtct gttagcccgc
    12001 agggtagagc ctgttgcaca gttgtcgatt atgatatctg catcctctgg agggaaaact
    12061 tctgttgctc cataagaatg cacaaatccc gcgccaggta agtttttgtc gatccaagcc
    12121 cttgctagtc cttcgtactc ggaagcgatg ataggagtcc ttttgcagac ggatatcatt
    12181 tttgtaagat ctcctctgta agcgcgaaag gctgttgcca cattttcggg agccgcggca
    12241 actatcttta ctggatcgag accggtatcc aaaagctcga ccaaatcggc tccaagttcg
    12301 cgtacccaat cttcacctgc aaagccgata tcgcggcttc cagcctctag catctcgacg
    12361 atgttctggg gttttagaag ctttgcttcg tacattatct ctctagatcc aaagaccagg
    12421 gcttttccgt ttccgattcg tggacgatat tctcgatcat cgatggatat aggtatgcct
    12481 gtatccttga aaagctcgat gacttttgcc tgcatcctgc cttttggaag gccgagcctc
    12541 aattccagct tttgatttgc attttctgcc attttttctc ctcctccttg caatctttta
    12601 tcaggcaagg aaaaaataag ccggccccgt tgggagccgg ctctggcaaa atcgacaaaa
    12661 aagccggctc cctggagccg gcttcgattc aatattaagc tgcgtttatt cccgcgccta
    12721 accacggctc catgtgaaaa agccgtgatg atgatgaaga ttaagcgctt tcttaacaga
    12781 tgtccctttc atattttcgt aatattgcag tttcttccaa aagagtcaag agggggacaa
    12841 tgtcaataaa taaacttcat gctacatgct aaagaaggca gatcaacaag gaagtcatcc
    12901 agcaaatccg catggaggct tgctataggt ttaattttta ggtttcttta ctttgatgtc
    12961 taaacctccc atcaaagtga tagcatttat tttgatagtt ggcgctccaa gctttgccga
    13021 tgaaggagcc cgattatcta tacctcccat aataggaatg cccgtgacct ccacattgac
    13081 cccttctggc acaaagatat ctactcctcc cattagggca aatacatcta tcgtagaaac
    13141 cccaggggga atctgagctt gtgagtagtc caggtcgaca ccacccatta tagcgacgac
    13201 tttcatcctc ttgggtggat accataagcc ctttctagtc gcaccagaca aaaaagctag
    13261 ggtgacatca taatccttga tttcaccgtg gttgagcaat attccatcaa gagatgtaaa
    13321 ttgtttattg gtctcggttg aagccggaag atcttcaatt agagatagaa gttctctttt
    13381 cgatgcggtg tttacagcaa tctccattcg cttctcgtat acatcgatat cgagcagatt
    13441 ttgcgcaaaa ccgtgctcta agcgctcgat gacattattt ctctcgattt ttgtgggacc
    13501 atgatcatac ttttctagct ctggcattat gacctctcca gaaactcaat gatattatgc
    13561 gatattatgc caataatctt gctaaaatgt aagcatgaga agcataagct aatctaatat
    13621 acaggatttt aacccatgga atcattgcct ctcgccaaag cccgatcaaa acattattat
    13681 catctcatac tatggcagaa aagaagattc gttgcagtac gcatatgagc aatctttgcc
    13741 aggaaaagga tggcaatcta catgacttcg aattgcatcc aaggggcagg ggaggggcgg
    13801 cgatcatcta tccggttatt tcgcgacgag ctggagggct ttctttaggc atcaaccttt
    13861 tccctgagcg caagatttgc agctacgatt gcccctattg tgaggttatg ccattttcca
    13921 atcctggggc ttcgctaaga gccggcgatg tgagccgcgc tatcgagtgt ttcttcgatg
    13981 aagaatggcc cgagctctca aagactctgg agctcaaaga tatttcgatt tctggaaatg
    14041 gcgaacccac actctcgccc ttcttggaag aagctttgta tgctgctcgc caagcaattg
    14101 atacaaaggc cctcgtaaag gattgcgcaa caccatcagg ccagcactcg attccatcta
    14161 tcgttcttat caccaattcc actggcttcc ttcgtgaaga tattaccgag ctccttgccg
    14221 gtttttataa aagatacccc ttcgaaattt gggcaaagct cgatggtgga aaagaagagc
    14281 ttcatgctct cctttcgcgc tcgcgatttc ggtttgctga aattgtccgc gggattgtgg
    14341 acttttcatg cagtgtgcct gtcactctgc agactatggt gatatatgac aatcgaacag
    14401 gcaatattta cttcgattgc cagtcctatg ctgtcacgat tagagaaatt ctcaatggtg
    14461 gcggacagct aagagcaatt cagatctata ctacggcaag ggttccagtc gaatcatggg
    14521 ttgcgcccgt gtcggatgag agactagtga tttttgcacg gcaattatct tcaatattaa
    14581 atagacccca aagaaagaag gctaccggca aagaagagga agcattacaa aatcaggtgt
    14641 attcgattcc cattttatgt tatggatcgc ggggcaaact ggattggaac catgccaacc
    14701 gctgaccttc acacccactc cactgcctca gatggtgagc ttgcgcctgc cgcgctcgtc
    14761 gaaaaagccc gcgcgcttgg cctttccacc ctcgccctta ccgaccacga taacactcta
    14821 ggagtagcag aagcgcgcgc ggctgccagg cttgtgaacc ttgaattcat ccctggcatc
    14881 gagatcgaga tagatttcga gccaggcgag tttcatcttc tcggttatgg gatcgacgag
    14941 gcagcgccac ctctcgtaga ggcggtggaa agactttccc acgctagact ggagcgcaac
    15001 ttagccattc ttgatcgcat tgcgaaagcc aatcttccac tggaccttga tagtattgaa
    15061 gccttttcga aaacctctta tctgggtagg ccacacctcg cagatctttt tgtggaagcg
    15121 cattgcgctc gtacgcggca ggaagccttt gatcgatatc ttggcaaagg gaaaccattc
    15181 tatataaaga aagcatgtct agcgctcgaa gaagcgctat ccgttattga tgctgcgggt
    15241 ggggtttccg ttcttgctca tccctattca ctttttgtat caaaagctgt tcttggcact
    15301 cttttttctc aattgaaagc caaaggcgtt aggggaatag aagcctatca tccaaccgcc
    15361 aaatatggtc aatgtgttat tctggaacga atggcacacg agcgaggaat gtttgtcaca
    15421 gctggctcgg attttcatgg caaaacgagg cctgaatgtg ggctgggtcg gacttcggga
    15481 aatgagccta tagatattcg ctttcgagat gagcttgtag ctgctcttga gagtcgtatt
    15541 tgatattctt ctttgaagat cgctgctcat cttcgccaaa tctcaaggcg ctctcgatat
    15601 tattgtctat tgataacaat atgccttgat aacgatagca atcaagaata gaggccttta
    15661 gaaacagctt caagcattat ttctcgtttt tattgtccga aagttcactt tcctcgtaca
    15721 atgccctata gagatcacca gcggcaacaa atagtatcag aataacggga cccaggatca
    15781 gcccgttaaa tccaaagact gccaggcctc cggttatgga gaaaaaaagc aagagtggat
    15841 gtatctttat ctgttcaccc agcacaattg gcctaataaa attatcgagc gtcgcaacga
    15901 ttacgcttgc gcaagcgaca aagacaattg cctttataat ttgaccgtta accgcaagat
    15961 aaatccccat tggagcccat actacggtag tgccgagaag cgggacaaag gatgaaatgg
    16021 ctgtcaacac cgccagcaca agattattct tgaaaccgaa gataatgctc aggatgaaca
    16081 tcataaaacc ttgaaaaagc gaaacgagaa aatacccgat aacaagttga cggcctgttt
    16141 ctcggagctt gctcataaaa agcctggtgt actgaggagc tataggcatc atggaaacaa
    16201 aggtgtctcc caaagaacta gcatccatca agagataata gagagtaaaa accatgaaac
    16261 agatagtaac cacaaacttg gagatattca ttatgaggga agtagatatt tttataagtt
    16321 ggtttactcc ggatgctagg aatgactgta gctccctcag cggatcgaaa gtactaacat
    16381 catatagatc accaagcaga gactgaattg ctgagcctat ttgattctga ggatcgattc
    16441 tcaacttatc gatattctcc tctacaaagt ggatgctcaa tttgagcaaa tccgcaagct
    16501 gttgtatcgt cttgaatgcg agaaatgcaa gaggaataag aataataatg acgccgaaaa
    16561 tggataaaaa caaagcgcta agtcgagagc gcatggtttt tcggcctttt atactattag
    16621 gacctctgag tcttttatga attggctcga gaaagacata caataagcca ctccatagta
    16681 ttacagttag aaaaggataa aagagccttc caaccagaag caataatgcg aggaatagga
    16741 tcaaaaagga aatctttcgc ggattcgtgg tcttcattgt ccgtaatgat aattgttatt
    16801 gaggtttatg aatagatata tcttatctgg agttgcgcta aatcgcagtt gggaccgtag
    16861 ttagaattgt atttggaacc atagttgcat caaagatatc gaggcatcta aagataagcc
    16921 tagacataat agcttgtaat cgtacacaat tgtctctgtg agcacacttt tcatgattgc
    16981 aacacctatt ggtaatctcg aagacataag cctgagagcg ctagaaacgc ttaaatcagt
    17041 agatattatc gcgtgtgagg acaccaggca cacattaaag ctgctttcgc gatatgaaat
    17101 tcgtaagccg ctaatctcat gtcattcgta caatgaacaa caagcagcca aaagggttat
    17161 agcccttttg agcgagggca aagacgtggc ctattgttca gatgctggaa cacctgggtt
    17221 ttctgatcct ggagcgctcc tggtgcgaat ggcgcgatca gcaggataca agatcgtacc
    17281 cattcccgga ccaagcgctt ttgcctgcct tatcagcgca tctgggctat ctgggaagac
    17341 ttttttattt gatggttttc tttcgccaaa accaggcaag aggcggacaa ggctaaagga
    17401 acttcttgac cgcggagaat cctttgttct ctatgaatcg ccttttcgaa tcgggaaact
    17461 gcttgctgat atagcctcaa tagatcccga aagaaggcta tgcataggca gggagctaac
    17521 aaaaatacat gaggagataa tcgaaggcaa ggccgaagag ctggttcagc gatttcctgc
    17581 ggaatcggcc aaaggagaat tttgtataat tgtgcatggc ataaagcccg ccacttctgt
    17641 gaatgacaaa gaagatgaat catgatggat atctctcact ttaggaatct ccatggtcgt
    17701 gctagattgc gaaaagccgt gcttttgctc gagaggctcg aaagagaatg gcgtaaggcc
    17761 gatcctctta taattcctta cgaagatctt gtttttgtca agcaaatagc taagattctt
    17821 gctgattctg aagagacgac attggaatgc gtgaaggctg ccagcgattt tcttgggtct
    17881 ctgtctgggc tcgacgatat tcttgattat tatggttttt tgcaaactga aaacaaattt
    17941 cggcatagac ttatgattct atccggccag ggcgtcgcag attgggattt catagccgtg
    18001 cccaggtctt cggctggtca tgccacaaag agagcgggca tgcgagtata tttggaagat
    18061 ttgcgctcgc cttttaacgt ggggagcatc tttcgcaccg cagaagctct tggtttcgaa
    18121 gagataatta tttcgccgga atgtgcggat cctatgcatc ccagagcaat ccgaagcgcc
    18181 atgggaacca tagagcgcat gaaatggcga agagccgagc tgtctgagct tgattgtatt
    18241 cagggagttt ttgctcttga gcttggcggt attgctatcg acaagttctc ttttccattt
    18301 ccagggttga tggtcgttgg ctctgaagag cttggagtct ccaataaagc gctgcaatat
    18361 tgcaggttgg gatgcatcga gattcctctg gttggaacaa aggcttcgct gaatgtagcg
    18421 acagcatttg gtattgcagc gtattcatgg tattattctg gaaatatgca ggcatgatgt
    18481 gtttatattt tcttgtgcta ttttgccacc tgcattaaaa ctatattagc gaaaggaatg
    18541 cagaatgtct ttaaatcctt ttatgaagaa gcttggtctt tcatcttccg acagggcggt
    18601 catagtccac gtagatgatg tagggatgtg tctttcatcg atagaggctt tttttgagct
    18661 atacgatttt ggtactgtaa catctggagc actgatggct ccttgccctt ggtttccgga
    18721 ggtggcgcgc cgctgcgcgg cgcgccctga tgtcgatgta ggcgttcatg caactctgac
    18781 gagcgagtgg aaaggctacc gctggggacc agtatcgact cgcaatccag attctggact
    18841 tctggatgcc gaagggtatt ttcccatgac cagtgaggag gttcaatctg gagcgaaacc
    18901 cgaagccgca atgaaagaaa tcgcggctca ggttgcaatg gtatatgctg caggcatcga
    18961 gcctacccac atcgacactc atatgttttc ggtagcgcat gaaaagcttt ttgacgccta
    19021 tatatcccaa gcccttgaac gtaggatacc ggcgatgctt ccaaagccag atatcgtggg
    19081 tttcgagtcc attgttccgg attgcgaaac ccggaagtac cttgctggat atcttaaaga
    19141 gcttggggaa aaaggcatgc ctctgatgga cggcattctg gcgatgccct tagatgaacc
    19201 tcgcgatcag accgagaccg tcaaacgcct tcttggttcc attaccgaag gtattacgca
    19261 tttcgtgttg catgcctcaa ccgacagcca tgagctgaga gccattacac cagactggga
    19321 aagcagagtc tggaattaca gaaccatgca cagcgacact atgaaaaaaa tcatagagag
    19381 cgaggggata gtcctaattg gctatcgcaa cctgcgcgat gcaatgagga aatgatctgg
    19441 acatctccga agctgggtga aggatttatg catagaaaat caagctgggg tatacgataa
    19501 gccttggttg acgcctttta taaatagtcg taaactgtct tcataacata cacctcttga
    19561 aaggaggatc catggcaaag gttgaactca agaatattgg aaaggtgtac gaaggcaatg
    19621 tgagagcggt ctcagatgcc aacatcaaca tccatgacaa ggagttcgtg gttttcgtag
    19681 gcccttcggg ctgcggtaag acaacaacgc tccgaatgat cgcagggctc gaggatatca
    19741 ctgagggcga aatatatatc gatggtaggc tcgtcaatga tgttccgccc aaggataggg
    19801 atatagcgat ggtttttcag aactatgcgc tctatccgca catgaccgtc tacgacaata
    19861 tggctttcgg tctcaagatt cgcaaattac ctaaggaaga gatcgataat cgtgtcaagg
    19921 aagcagcgcg aatcctcgat atcgagaagt tgctagaccg caaacccaag cagctttcgg
    19981 gtggacagcg tcaacgagta gcagtaggcc gagccatcgt gcgcaagccc aaggtttttc
    20041 tttttgatga gcctctctcg aacctcgatg caaaactgcg tgtccagatg cgtgccgagc
    20101 taatcgaact ccacgaccgt ctccaatcta caatgatcta tgtaacgcac gaccaggtag
    20161 aggctatgac aatgggcgac aagatcgtcg tcatgaagga tggacttatt cagcagatcg
    20221 gttctccttt gtatctctac aataatccga tcaacaagtt tgttgcagga ttcataggct
    20281 ctccacccat gaacttcatg agcgtcaatg tcatagaaga aggcgggcag gttttgatcg
    20341 acgaaggatc attcaagctc gctcccgatg cgaagcatgc cgagcttcta aaggcttatg
    20401 ttggtaagag tgtctacttt ggtatccgac cagaagacct tcccgtggcc gatgctgtac
    20461 atcgtggcca gaagttcaat gcaaaggtta cggtggtcga gccgctcggt tcggagattc
    20521 atcttcaggc aaccacgcca actcaaggca tggtgtctcg tattccacct catcaccttt
    20581 acaaacacgg cgatacaatc atttttgctc cggtaatggc aaaagctata tacttcgata
    20641 aggaaacaga gaagagcatt cttccagtaa aatggaacga acaagaggcc taaggctaag
    20701 aacgatagcc aaagctctgc tattataaac cgactttatt gatttctttt tgtggtaatc
    20761 aattgaaaat gaaaagccgc ctctatttaa ggcggctttt ttagggccaa tcattcgttc
    20821 tctttagatt cttttttagc gcgtttctca gcacgctttt ctttgagtgt tttttggggt
    20881 tttttcttat cggccgtttt aggtttatcc atcgatttca taccgcacct cttattctta
    20941 ttatcgaaaa aactgcagag cttttcaatc acttctgcgt tgcgttctga atttccgaag
    21001 gttcaccttt tgtcacttta cccattattg cacgagcgca aatcatacag attgtacaga
    21061 agaggaatat tccctggtat ccgaaaatat cgattatgac gcccgtcaat acagggccta
    21121 gaatagccgc agcttgactt gcggtataat aaaggcctgt gtagattcct attgtgcccc
    21181 attctgccat ctgccatagc atagggaaag agtttgttat tattgatacc cagaaaatcc
    21241 cgaaaagaaa catgatcgcc cagaatgagc ccaaccttaa ggaatttgat agaccagcgg
    21301 taatccattt gtgaaaaaat aggagaaggg tgagaatcga tatcaccaaa aggcatgtac
    21361 gaattgtctt ttttctgcca aagcggtgtg cgacatagcc cgaagggata gcgaatatgg
    21421 cataagctat gcccaccatt ccggctgcca gagccgccat accgcttgaa gttctgagca
    21481 catccaccga atacttgccg ataaatggca agacaccctg atatcctaaa aaccatagaa
    21541 atattgcgat taaaatatat agcgcgctct tgtctttctg cgcggcaaca tttcggaagc
    21601 tctcgagaat aggcactttt tgttcttgtg tttcctttgc atccggctgc ttctcacgga
    21661 tgaatatgaa aagaaggatc acggctaaaa tgacgaacag gccgccaagt gggaagggga
    21721 gcttatcctt ggtaggtcca aggataggca gcgttatatc gagatccatt aatctcgcaa
    21781 ggccgattgt tcccacaatg cttgctatgc cgcccattgt gttaatgaca ccgttcgctt
    21841 cggagcggta atcggccggt atcgtatcag gcatgagcgc aaccactggt ccgcgcactg
    21901 actgctttgt tatatttaga aggatcaatg ttacaagcaa agccacgaga gatacttccg
    21961 tagcataggg tatagcgcca aaaaggaaag cggtaagggg caaaagaacc agaatgtagg
    22021 gcatccgacg gccaagcttg gtataggtat tatcggacca ggcgctgaca agcggtatca
    22081 aaaaaatcgc cagtacattg tcgagcgtca tgaaaaagcc gacaagactc attttgctta
    22141 ggtatctact gaggaatatc ggcacgtagg tatcgtatag gggatccata agacccattg
    22201 tgaagaagcc gaatccgatc aaaaaagtta tgcccagata aggcttaaac ccttttttct
    22261 cgctcatcat aaccctcctt gctagtatta atagagcatt taattcataa gaaccttaca
    22321 tacaccaaat ggccggatat taatgcgctg tacgctgcct tttccaaaga ccgcctccat
    22381 acgctcaaca aattcaggga atcttgactg tggaacatag acctggattg tacctgcaaa
    22441 accgccgcca tgcacgcgcc atgcgccttc cggactcaat agatcttcgc taagcataag
    22501 ggctatagaa aggctctgct ctcgcgggtt gccagcatgg atattctgaa ggtaatgcca
    22561 gctcgatctt ccggattcat taacaattga tagataggca gatttgtcat ttcttttcaa
    22621 ggcttccacc attcgtgctg gacgatagtt ttcataaacg aaatgccagg cgcgcaataa
    22681 agctctatct ccgcatttta tgcgtatgtc cgaaagccta ttgagaaggt cttgcttttc
    22741 tgttcctcgt agagcagttc ttcccaggat agctgcaaca catttcattt ccttgggaat
    22801 gtctgcatat tctcttgtaa gatcctcgtg attggaacct gtattgacga tggccagaat
    22861 aaggccatta gattcaaagt cgtatatcag tctatcgatc tttggccttc gagaatcggc
    22921 gaagtctatg aatacgatgc caccttctgc gcatgcaatt tggtccataa gcccacaagg
    22981 ctttccgaag tattcatttt ctgcccattg tcccgctata gcccaatcgg ttggtgtcaa
    23041 atcatacttg cccatgtcgt cgaatatcga tgcaatcaaa agctcaaaag ccgcagagct
    23101 cgacaagccc gagcctgccg gcactctaga gtccattgca atatcgaaac cagggccaat
    23161 ctgcaggcct tggtgagtca gccaagctgc caggccgcgt attatcgaag caggtgaacc
    23221 tttttcctca attctggggg cggtatccct caagtcgact cttatgggag agtagccttc
    23281 agataaaagg gtaaccattg aatcgctgcg cgggcgggca agagcaagca tgtcgaggtc
    23341 tactgaggct gcaagaacac agccattgtt gtggtcagta tggttgccac ctaattctgt
    23401 gcggccaggc gaagaataat aatgcaaagc ccgagaatgg ctatcttcgc cgagaggtaa
    23461 tctagataca agcgaatcgt atctggctct gcgtccttcc ttttcgagat cttggaatga
    23521 ggcaagagga atgctaggaa tcttctgttg gatcatggct tgctcttgtc tattgtccta
    23581 gaaaatcgag ataaagggaa gagtcgcctt ccttgtattc aagataaata tcgcaaaaga
    23641 gcctctcaga acgaaaaagt ctttttgcat tctcttggaa ttatgccttg ctcccttcca
    23701 agcctccatc aaaagagaaa tgctctaaat agataaagct acgagtttgc attgctatat
    23761 ataaacatat atttatatct atgtttattg tgtcttatat aatgcacatt gcggtgtgcg
    23821 gagaacggtg ctaaaatcaa aaatggattt ccaaatcgat aattcaagct cgaggggtta
    23881 atatgaatca gctggcggaa gaattgaatg ctatccttgc gggaactgtg gctgagagac
    23941 tgctttccga aatgggtagg cgtatgtatt ttcctagagg aatcatcacc cagagtgcgg
    24001 aagcaggaga aaaagcttat aggttcaatg ctacgatcgg catggcttat gagcatgggc
    24061 agccgatgat gctcgatgca ttgcgcgccg aattgcccgg tttgactcca acagaagcag
    24121 tcgcctatgc atccacaggt gggattgcag agctgcgtaa ggtatggaaa gaatcgctct
    24181 atagaaagaa tccatcatta gatgctaaaa agatatctct tcctgcggtc gtccctgggc
    24241 ttacagcggc ggtatcctat atttgtgata tgtttgtcga gcctggcgat acagtaattc
    24301 tgcctgacct acactggcca aactataggc tcatcgtaga agaaagaaaa agcgcagcgg
    24361 gcataactta tccactattt gctgggaagg gctataatat tgaaggcttg cttgagggtg
    24421 ttcgacaagc agggcagcgt cggggcaagg cgataatcat ccttaatttt cctaataatc
    24481 ctactggcta ttccttgacg cttaaggagg ccgatagcat tactgccggg cttctagaaa
    24541 tcgctaagca gggaacagat atccttgtaa tagccgacga tgcgtatttt gggctgcaat
    24601 atgaagatgg tcttctctgt gaatccatgt ttgcaaggtt agcggacatc caccccaaca
    24661 tactggctat aaaggctgat ggacctacta aggaagacta tgtatgggga ttccgcgttg
    24721 gttttgtgac cttcggtgct gctggttttg gcgatgagca ttatgaagct cttgctaaga
    24781 agctattggg agtaatacgt tcaagtgtct ccagttcgag cggtctttcg cagaatctcc
    24841 ttctaaaagc tcttaagtac caagggtatg aggcgcaaaa agcgaggtat agggatatcc
    24901 tgaaaggccg ttacaaggca atgaagaata aattggccga gatgacgctg ccggatggtc
    24961 tcattccgat gccattcaat tccggttatt ttatgagttt cagatgcgat ggcttttctg
    25021 cagaagctct taggaagaag ctcttgaacg agaaggggat cgggaccgtg tctcttatgg
    25081 accgctatct tcgtgtagca ttctccagtg tagaagagag cgatatttca gacctctgcg
    25141 atgcaatagc gcaagcagtg gttgagcttt gtcagcaagg ctgaggaaga attgtaccca
    25201 agagtgcgca gaactatatc tctttagcag tcatgcttga ctggtcttga gctgattgca
    25261 ggacatagga tttaaggggc gggaagccat tgaaatagat ggacgaatag ctcctggtat
    25321 atgcaccggt ggtcagaata tataccttat ctcccacctt agctgaagat ggcatgaaat
    25381 atggagtccg ctcatagagt atatccatag agtcgcatgt gggtcccgca agaattatct
    25441 cttcggctga gccttgacct tcgaaataaa tcggatactt gatcgattcg cccagtgtct
    25501 cgatgagtcc cccgaatttt cctatatcga gaaacaccca tggatatcta tcgtgaacgg
    25561 atttcttagc tatgttgaca atttctgtga caattactcc tgcatctcca gccatagaac
    25621 gccccggttc gattatgatc ttttctggcc ataccaaacc gaaactgtta tctagaaaac
    25681 gctttatgtc ctctgcatat tgctggagag gatcggttgg atccagataa ttggccggaa
    25741 atcctcctcc catattgatc attttgagat cgatcttgag gtcgtggcga gcccaattaa
    25801 atagctgggc ggctatagtg agtgcgcttg accactggcc tacatcgcgt tgctggctgc
    25861 cagggtggaa agatatgcca tatggttcaa ggccaaagcg aattgcggtc ttgattagct
    25921 gacgggcaag gtcggggtgg gaaccgaatt tttttgataa aggccagtct gctcctaggc
    25981 cttccgtgag aagcctaaag aacaccttac tgccaggtgc cgcgcgagag agtttatcga
    26041 catcgttaat cgagtctgtt acaaaaagcc ttacgccttt ttcatagaag tatgcgatgt
    26101 ctttttcttt ttttatggtg tttccaaagc tcattcggtc tggagatatg cccaggctca
    26161 gaagttgatc cagttcatat cgagaagcaa catcgaagtt agagcctaga tctctaagca
    26221 gagaaataac agcatcatct ggattggctt tgacagcata gtaaatagtt gcccatggaa
    26281 gatatgctct tagatcttcg aagttctttt ttatgatgtc taagtctatg atcaagaatg
    26341 gggtctcttt gtccctagag aattccttga ttctttcaaa tttctgacgg ttaataaagt
    26401 tttcgagggg aaaggaatag gcgtttttca ctgatccgac ctcttcgatt taagatagct
    26461 ctttagagct ccaagcgcat acattatgca tctacttgtc caaaggt
//
LOCUS       DAOOWY010000017        26047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_1877_length_26047_cov_9.538012, whole genome shotgun sequence.
ACCESSION   DAOOWY010000017 DAOOWY010000000
VERSION     DAOOWY010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 26047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26047
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1877_length_26047_cov_9.538012"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PK105_02625"
     CDS             <1..316
                     /locus_tag="PK105_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU60634.1"
                     /translation="LSLGQRQLISFARVLAHEPSVIILDEATSSIDTETEKLLQRGIE
                     GLLKGHTSIVIAHRLSTIRDANRIIVLGQGRVAEIGTHDELIALHGLYWNLYRLQNKE
                     ME"
     gene            317..1129
                     /locus_tag="PK105_02630"
     CDS             317..1129
                     /locus_tag="PK105_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOU60635.1"
                     /translation="MMPFSEEKEPFIIGQDDRVTAVYKPCGWHSVCQKKSDHSIVSWL
                     YDQGIPRLRESQMDQELGLIYRLDQMTSGILLFAANPQSLEELRQAQKELAIVKRYIL
                     ISTFSECELLGSLPGTLKEKQSCLIEELFSKREAYIESYFRPYGPKGASVSCIAAEFA
                     SKSKKPITKEIYVSKFIAAYRMIEESNARLRLPNEAVCLEVEIRKGFRHQIRAHCAWI
                     GLPILGDMLYGGQGSNRLWLEAFSVCLPQADRCRNEWKLYNGIQDIAQVSSE"
     gene            1288..1458
                     /locus_tag="PK105_02635"
     CDS             1288..1458
                     /locus_tag="PK105_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU60636.1"
                     /translation="MAYRVTEACTNCGSCESECPVSAISEKDNARFIDPETCIDCGAC
                     AAACPTEAIIAG"
     gene            complement(1561..2892)
                     /locus_tag="PK105_02640"
     CDS             complement(1561..2892)
                     /locus_tag="PK105_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOU60637.1"
                     /translation="MKKRKIIIIGAAGRDFHNFNARYREDESTEVVAFTAAQIPNIDG
                     RRYPAALAGRLYPEGIPIYSESELSDLIRKYDVDECVFSYSDVPYSHVMKMSAIVNSA
                     GASFALLGPKETQLKSSKPVISVCAVRTGSGKSQTSRKIVQMLMKRGLKVVAIRHPMP
                     YGDLVQQKVQRFATIEDLAHYRCTIEEMEEYEPHISRGNVIYAGVDYEAILRQAEKEA
                     DVILWDGGNNDFSFYIPDLQITVADPLRAGNEVSFYPGEVCLRTAHVIIINKIDSSSP
                     EQIQIVRENIARTNPSATVIEAASPIIVDRPELIRGKRVLCVEDGPTLTHGMMKFGAG
                     VVAARKFQASQLIDPRPFLSGGLTQTFNTYPDIGTLLPAMGYSDMQKRDLEDTINRTD
                     CDTVIIATPIDLNRIIHIKKPTVRVDYELQEIGLPNLEEVLEDFCKRKGLS"
     gene            complement(3173..6679)
                     /locus_tag="PK105_02645"
     CDS             complement(3173..6679)
                     /locus_tag="PK105_02645"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOU60638.1"
                     /translation="MKIKRLFTRAGYGPYEKIKWEKRKSEIKNPDGSIVFSTDSVIVP
                     SFWSSIASDILAQKYYRKAGVPKDKALEWTKFVPVSQTILSEEDMVDDAEHDARQVFH
                     RLAYTWCDWGQKAGYFDSEEDRQAFYDEICYMLAHQIAAPNSPQWFNTGLFSVYGIEG
                     PAQGHYFIDPDSNEMAKSESAYERPQPHACFILDVKDDLVNEGGIMDLVTKEARLFKY
                     GSGTGSNFSRLRAANEKLSGGGVSSGLLSFLKIADRSASAIKSGGTTRRAAKMVILDA
                     DHPDVEDFVHWKAEEEYKVACLAAGSGLLNHYAQAMISAIEDMGVQDENSYLPDRNLL
                     LGKAIRQALDAGIPSSWIHQVLTYYAQTGRTPRMPQYNTSWESEAYNTVSGQSSNNSV
                     RVDNDFINAVINDLDWKLIARTTRKPFRVTKARDLWNEIAESAWFCADPGLQFHTTIN
                     EWHTCSADGEIRASNPCSEYMFLDDTACNLASLNLLSFYNFETCKFDEAAYRHTIRIW
                     TLVLEISVVMAQFPSKEIARNSYDYRTLGLGYANLGSLLMVMGLPYDSDNGRALAAAL
                     SAILTGEAYTQSAIMAREFGPFARYEANRQSMLRVIRNHRHAAYDAAKEQYEALSVFP
                     KGLDSRSCPTNLLNAAHECWDEALKLGEKHGFRNAQVTAIAPTGTIGLLMDCDTTGIE
                     PDFALVKFKKLAGGGYFKIINTSVPPALHALGYSDSQIKEIIQYALGKGTFEGAPGIS
                     RQSLIDKGIPNDSIDRIEKSLQNAISLDSCFSPSIVGYDVLEKLGISKEEANAPGFSL
                     LSRLGYDEKQKKEAEIFACGTMGMEGAPHLKSEHLSVFDTATPSGKKGSRSISWQAHI
                     AMMAAVQPFITGAISKTINMPNNVSIDDIKGAYLLAWRSMLKSIAIYRDGSKLSQPLA
                     ALTPGSNLIADSIIALQSDELTSEDISSLEMVPTGLAHKSIEPELPGLPVAPRGVRRA
                     LPNRRKGYTQKAKIGGHSIFLRTGEYEDGSLGEIFLDMHKEGAAFRSILNSFAIAISL
                     GLQYGVPLEEYIDAFTFTKFEPNGIVSGHDYLKMTTSVLDFVFRDLAISYLHRYDLGQ
                     IKPEDLIATSTQSLSAKVPNAFESGKKHIPIKKDTDIMLIREAKQKGYEGDACPVCGN
                     LTLIRNGTCLKCETCGSTTGCS"
     gene            7053..7985
                     /locus_tag="PK105_02650"
     CDS             7053..7985
                     /locus_tag="PK105_02650"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOU60639.1"
                     /translation="MSISDLACSLKATLEYDPLVHAGTITKEGRSVRFAMDVPYVLLD
                     WTIVKEVPAPYESATSLQIKEEFARTVQEFFEIKSQISSRYLVKAIVIDPGHGGKDPG
                     AIGEFEDFKLQEKDVNLSIALRLADLLRLRYPEKTIVLTRSSDIYPTLEERVEKANKI
                     ELEETEAIIYVSIHANASFNKNTKGFEVWYLNPNYRRTVVDERTAKEKGEDIAPIINI
                     MLEEEFTTESIILAQKVYQRMDRIIGDRSPARGIRAEEWFVVRNAKMPSILIEVGFVT
                     NKAEALLLSQAGYLRQIADAIYNGVCDFIEHFEQ"
     gene            7987..8439
                     /locus_tag="PK105_02655"
     CDS             7987..8439
                     /locus_tag="PK105_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60640.1"
                     /translation="MNPSKEQDHKQGYIIALVIFLAIVFIVWKGPTLQYNSMRLYFPD
                     KKKGELRLEKRPIAPIGTLKEKAKATVEELLLGPLSRDLKILTDTDISLLRIVEGKNA
                     LFMDFSTQDIASFSSEYMTFKSALEKSLKETIPGTFHIYFFINGVLAR"
     gene            8601..9899
                     /locus_tag="PK105_02660"
     CDS             8601..9899
                     /locus_tag="PK105_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU60641.1"
                     /translation="MNDLENIPNKTLLEKLYNQHRTGYEEALAKTVNDISNLLQDKGI
                     HPTIKSRVKDFESYFSKKIRFLKKAWDEHKDPLPINDVLAMRIICPFLRDLEETVHLL
                     DQRYEVVEIERKGQDRSVREFGYESIHVLIRIPDELVPLCEGLERYVMEIQLRTILQE
                     AWAEVEHEIVYKAEFTPLDEPLKRKLAALNATLTLSDIIFQEILDYQKRLSDALKKRR
                     QGFYGKIEQKTAEIFGISEEITEPIPSFSGQMMEQRETAYPNENQVSASNRSNGLDMD
                     MLLLSALNAHNASDFNSAISIYTKILDKKPEASLAAVVYKHRGMAYFAQSEYEQALAD
                     FTSCLILDPNCYKALYYRGVVKSIQKELPQAIEDFSKALEIHPYHFFSRYRRALCWWQ
                     LGDYAQAHADCELALRIMPENKLAQELQKKIMEHLAKEDI"
     gene            10032..10103
                     /locus_tag="PK105_02665"
     tRNA            10032..10103
                     /locus_tag="PK105_02665"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10065..10067,aa:Glu,seq:ttc)
     gene            complement(10141..11328)
                     /gene="thiI"
                     /locus_tag="PK105_02670"
     CDS             complement(10141..11328)
                     /gene="thiI"
                     /locus_tag="PK105_02670"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOU60642.1"
                     /translation="MHDLFLIKPGEIELKLGNRREFTRRLKEQITKRLKGIPFIFEEY
                     PGRFFLTVEEKDSELALFVLQHCPGINGVAKAIRIKKDIDEICQATVECAKTEISAGS
                     HSFKIEARRSDKSFPLDSYKICVVAGNSLLSEYPKLSVDVHNPDFIIRIEIREQAYVY
                     SRTMTGPRGLPVGSQGKGVLLLSGGIDSPVAGYMMACRGLALESIYFHSYPYTSPEAQ
                     QKVERLAARISIWTGGMNLWIVPFTDIQLAIAKASYEEAKTLMLRMAMMQAADIIAHR
                     INANSVVTGESLGQVASQTAENMRLSQSKTSLPVLRPLIGIDKEGIIAIARQIGTYDI
                     SILPYEDCCVLFSPKHPILKPDFGELRSHFESLALGNLIESAVDNSERKCFDFADALK
                     SGI"
     gene            complement(11342..12523)
                     /locus_tag="PK105_02675"
     CDS             complement(11342..12523)
                     /locus_tag="PK105_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOU60643.1"
                     /translation="MKDIYLDWAATAPPDPDIMRIALEFSLKAYGNPSSKYWLGKEAR
                     QALEESRLRVMRSMGVTKGSLIFTSGGTEADHIPIFAILEKLKRASSAEKPMHAIISS
                     IEHSAIEEQMRILERLGLQVSRIDPDSKGHIEAEQIESRIRKETALICIMNVNNETGA
                     IQNIKAIAESIKETVLQKKIKKPWLHVDAVQALGKLSIRDLEDHADSFAISAHKIRGP
                     KGVGGLWIARPLEAFIRGGGQESGMRPGTENLFGALAFSLAAEKAALSMQENQEKAIA
                     LEKRLLDGISSIPNTLTLPERIPQDKGFVPSIISMAFPGLGGETMVRALSDCHIAVST
                     GSACMSNIHLAERRVLKAMLVPDDIAFSSIRVSTGPLTTPQDIDSFLEQAEVLYRRLK
                     T"
     gene            complement(12531..12833)
                     /locus_tag="PK105_02680"
     CDS             complement(12531..12833)
                     /locus_tag="PK105_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOU60644.1"
                     /translation="MNLENISIMKKEGMRKDHEIMVYALSTCIFCKRALTFLDVNHFA
                     YKYLYIDLVPLESKNAIKNELTQRFKENVIFPFMVIDSREHLIGFIETEWKMALGA"
     gene            12961..14775
                     /gene="lepA"
                     /locus_tag="PK105_02685"
     CDS             12961..14775
                     /gene="lepA"
                     /locus_tag="PK105_02685"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOU60645.1"
                     /translation="MIEPAFIRNFCIIAHIDHGKSTLADRFIEKAHLIDSRKFQDQML
                     DNMDIERERGITIKSQAISLPYVAKNGKTYMLNLVDTPGHVDFSYEVSRAIAACEGAV
                     LVIDATQGVEAQTLSNMYMALEHDLEIIPVINKIDMASADIEGVRGQITKDLGLEGES
                     ALAVSAKLGIGIDELCEAIVERIPSPKGGAENPLQALIFDSHYDPYRGAIVHLRLKEG
                     SIKKGMHIRLMSTGAVHEVEEVGHFRMALSPAMELSAGEVGYCIAGIKTVKEVRVGDT
                     LTDHENPCNTALPGFREVKPVVFSSIYPMDSSDYEELRDSLEKLVLNDASLIFEKDHS
                     LALGFGFRCGFLGLLHLEIVQERLEREYDQSIIMTAPSVRYKVILKDGSTLYIDNPSD
                     YPDPSRIENVEEPYIKAEIITPVDYIGNIISLCVSKRGSQQSFLHLDERRVELIFEMP
                     LAEVLFDFYDKLKSISRGYASFDYVVTGYRPTDLVKLDFLINGEPVDALSMLVYRPNA
                     YDRARVVCERLRNNIPRQQFKIPIQGSIGSQIIARETVNPVRKDVLAKCYGGDITRKR
                     KLLEKQKEGKKKLKIIGNVELGKEVFLSVLRSSEEEDR"
     gene            complement(14838..16442)
                     /locus_tag="PK105_02690"
     CDS             complement(14838..16442)
                     /locus_tag="PK105_02690"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOU60646.1"
                     /translation="MKGQRMASEENEKQAKTGIKKSGDKKGDNKQTKTTLKKTIKNPF
                     VYIGTIIILVLTIIAFVLIPSISSGTGAGAKLPSFGSWNGNPIQYTSGSYFAQQVSQI
                     NDYLRQQGLDETQSQLYAYQVWRLAFQNTVIRTAILDSAKRSGMKVSEETIDEEISKN
                     SQFQVDGKFSLEKYNSTPAATRLSIRNSIREDMLIQNYYNDLMSASPSTAEIEFVASM
                     AKPQRSIQYVNISYADFPVEETLEWAKSHESLFRTIGLSRITISTSEKDAKKVLSQIK
                     ENSLSFEDAAKSHSKDVYADKGGDMGTKTFYDLKGEFLDEKDAENLMSLKKGEISPVY
                     KQTGNTWAFYRINSEAKAPDFSNQSVLDEVKSYIFDREKSVLESWALAKANEFKAELM
                     NESFESAAKKVGLQVKPAGPFILNYGNPGFYFYGQQVSLFQEPYRSLDTDLVGAVENE
                     TFLTTLFSTPKDAVSEPVLLDASVVVMKVTEDREASDQETSYTKFSYPYFFQTAMENH
                     IRNSILTSDKFKDNFNATFAKLFVSN"
     gene            complement(16483..17409)
                     /locus_tag="PK105_02695"
     CDS             complement(16483..17409)
                     /locus_tag="PK105_02695"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60647.1"
                     /translation="MTSENRYVLYFQVKGTCPYLSNNHSTMMNLLIFQDEVDSRLYSA
                     LISNGWRRHGKEIYRMECPGCKLCIPLRIKAESIQLTLSLNRILKKNNDIIIISNPPE
                     FDEEHFRLWQRYSIWKHSIPLKDLKEDSYKNLLEPWSLIFEYRDNSKGRKLLAVSHVD
                     PLDDGLSSVYFSFEPDARGRSLGFFSILAESYLSSFAKKAKHLLLNKEIKGESVSHFY
                     ENRIQHKLGRDDAKSQAADNDSEDKRYYYLGFWVPGASKMDYKARISPFEIAIADENI
                     DDPNPWKRFLSEKEAIAYLSKQKWPGLSLTIP"
     gene            17515..18036
                     /locus_tag="PK105_02700"
     CDS             17515..18036
                     /locus_tag="PK105_02700"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOU60648.1"
                     /translation="MSIITKTGDEGTTGLWSGERIGKDSPRVEAYGTIDELSSALGMA
                     RHLCMQDNVLCAIEYIQRLLFRVAGELASMSMPFDNPINEDDEKTIEDKTNELEMRIP
                     IRSFVMPGMTGGSAALDLARTIARRAERRVVALSRIEHVSPVLLRILNRLSDYIYMLA
                     REEESKQGKLTFI"
     gene            18113..19384
                     /locus_tag="PK105_02705"
     CDS             18113..19384
                     /locus_tag="PK105_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU60649.1"
                     /translation="MRLYSKAQIGLVIVVSVTLTALSIAGIVFVLDRSDQNQSTMAVA
                     SKIAQDGDEAVYIPEITSLGIQDDKTASLSLQVANSTLSMSSYSADEIENITIYEKYN
                     ESVVNINTEVLGINWFLEPVPQSGGSGSGSIIDEQGYILTNNHVVEGAYKLYVSLADG
                     SRYEAKLIGTDPESDLAVIKISPEPGKKLKPIPFGTSKDLKVGQKVLAIGNPFGLERT
                     LTAGIISGLGRPIQESETTILQNMIQTDASINPGNSGGPLFNMKGEMIGINTMIYSPS
                     GGSVGIGFAIPVDTAVRIAPQLIKNGQVKRGWIEMQVIQLFPDLVNYLRQSGKAAPVE
                     TGLLVSTVTSGGNAEKAGLKGGTTAVRYRSTVFYIGGDIIVSIDGKPVTKIAELYAAL
                     EDTSPGQRVNVEFYRGSKRMSTVVILAERRR"
     gene            19381..21360
                     /gene="uvrB"
                     /locus_tag="PK105_02710"
     CDS             19381..21360
                     /gene="uvrB"
                     /locus_tag="PK105_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOU60650.1"
                     /translation="MKPFKVVSPFEPAGDQAQAIETISSAIKRGEKYLTLKGVTGSGK
                     TYTMAKVIEAIQKPALVISHNKTLSAQLYREFKEFFPENAVEYFVSYYDYYQPEAYVP
                     SKDLYIEKDSDINEEIDRMRLSATASLMERRDVVIVATVSCIYGLGSPDLYREMRLYL
                     DKGQIVDIDEIKRNLISLQYERNDMVLERGRFRIRGDVLEIFPAYLEEAYRIELDFDT
                     IVRIRRLDPLTGEASNELEEAIIYPAKHFVMPEDLVQKAVDSIRNELEERYQFFNAHG
                     KYLEAERIKSRTEYDIEMLSEMGYCSGIENYSAPLSNRKPGERPGVLLDYFPKDFICF
                     IDESHVTLPQLGAMYNGDHSRKLSLVDYGFRLPCALDNRPLRIDEFESIVAQTIYVSA
                     TPGETELRRSKTLAEQVIRPTGLVDPEILVHPTEGQMEDIYARVRERARQGERSLIIT
                     LTKKMAEELTDYLSELGLRVRYIHSEVETIERVEILTQLRAGEFDVLVGINLLREGID
                     LPEVSFIAILDADKIGFLRSETSLIQIIGRAARNAAGLVVMYADVMSPAMKNAIMETE
                     RRRKMQQAYNLEHGITPRTISKKVQDILERHKQERIEASHGEIEMLRRTHNLLVPEQR
                     RSLIKELEKLMFEHAKNLEFEQAALIRDEISKLKE"
     gene            21375..22376
                     /locus_tag="PK105_02715"
     CDS             21375..22376
                     /locus_tag="PK105_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOU60651.1"
                     /translation="MSNNHNQEDFYVIDAHCDTLMAIVGRSMNKEERDPRNFFADNNS
                     VHLDLPKLSRGHVACQVMAIFLEDSQLSEATREAMAMIDSLESLLRFDPREFFLARNS
                     ADIYRALETKSVCALLSIEGAEALGDSLESLEIFYQRGVRAIGLTWNRRNAFGRGLKA
                     EGSDGLTNLGKELVEKMEEIHVIIDVAHLGEEGFWDVVDISTGPFIASHANARDILDI
                     PRNLSDSQIRAIADHGGAIGCVFVPGFVTKDPGDCSLEAFLLHIDHIVRVGGIESCAI
                     GSDFDGFMPQGGKVIQDSGEFPLLYEALRSHGYDHAAAQKILGGNWLRIFDDVLGLV"
     gene            22373..22915
                     /locus_tag="PK105_02720"
     CDS             22373..22915
                     /locus_tag="PK105_02720"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOU60652.1"
                     /translation="MKKIALIGMMGSGKSEIGSLLASNYGIDFIDMDRAIEEHTKTTI
                     PTIFRDFGEEHFRDLEQNQLALMAVSFSSLVLSCGGGIVLRDSNRLILRDSFLTIWLD
                     VSITELKRRLQNQRHRRPLLLSDEWESQIEEIYRQRYELYRSTARIQYTWKDNHSMEE
                     SAIDIKRLIDAAYNGISGRT"
     gene            complement(22888..23982)
                     /locus_tag="PK105_02725"
     CDS             complement(22888..23982)
                     /locus_tag="PK105_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60653.1"
                     /translation="MSLEVNNLLIERDSFALFADFSLEEGKILALLGPSGCGKTTLLR
                     SIAGLEMIKEGTITLNGIRIDRLPTEKRHIGFVFQDLALFEQLRVRDNIGYSLFIRSE
                     EKRKREERVISLARNFKIEHLLGRYPFELSGGERQRVAFARAFASDPLLMLLDEPLSS
                     LDAPLRREMRRFIREQLSKQRLTTIHVTHDVEEAIDLGDEIIVMRDGKMISRGIISDF
                     EKKPGSGWLARFMNLGLVLPITEISASKESQLLNIMTSEGNTFQVSRNSVSNIDKALE
                     KAMCLYIPFSAIEIFGKNNNRKTMKVKIVRLISATPLASKLVLTIPEMQDYFFELPMP
                     ISDFSMSSQGKEIEISIDQGMCQVLPEIPL"
     gene            complement(23972..25633)
                     /locus_tag="PK105_02730"
     CDS             complement(23972..25633)
                     /locus_tag="PK105_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU60654.1"
                     /translation="MRAKIFLYLVLCIILIFSLAPLIAFLHAAIVGLSEIEGNIGGIE
                     IILEFLKQIPGSTIAKSLWFTIRQSFASTALAISLGLPGAWLAARFRFPGRKTLLSLA
                     AIPFCFPPILIILAFICYYGKEGLLPTALSLVFGSRGEYRGILYSFWGLVIVHAFYNF
                     PIVVHQISALLLRIPNTSFEAARTLGARKSKAFRTGILPWILPGIGQSAGLIFLYCFF
                     SFTTVLVFGGRAGTTLEVEIYQALRYRSNLALAAVLSIIESACALLGIWMLTRMQSKT
                     RGTVRDFGAAHKLAKPKGKQWFLIVLYEILIIVFFIGPLFSIVIEAFTLPASLNSPER
                     FGVDNFKRLFTGFQAPLTGAILSTLKLSGIAALLATSIGILASFSAFLAQRIGKKSML
                     IDLMMDLLQWLPMAVSPAVLAYGWLFISANHIATYALIAAQALIAWPFVSRTVGASLR
                     AIDFRIHEAAKTLGASPLRAFMTIELRTILPSVAAAAAFAFSITAGDVNVPLMLGMSE
                     IETLPLLLYRLTAAYRFNEACAAGLVLGLMTGIVFMLKEKAVDVA"
     gene            complement(25630..>26047)
                     /locus_tag="PK105_02735"
     CDS             complement(25630..>26047)
                     /locus_tag="PK105_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU60655.1"
                     /translation="PSVLAMTPSWDTGYGLFTKGEAPLVLSYSTSPAYHKAFENSERY
                     QALQFVEGHPTQIEFAGVLISSKKRELAQEFIDYLLSAKCQALLPETQWMYPANIKTE
                     LPPSFSIVQKYPALSATIRDLDHDPSEAADLLIGNP"
BASE COUNT         7320 a         5516 c         5906 g         7305 t
ORIGIN      
        1 tctttcgctt ggtcaaagac aactaatctc ttttgcaaga gtccttgccc atgagccatc
       61 tgtgattatt ctggatgagg ccacaagttc tatcgatacc gagacagaaa agctacttca
      121 gagaggcatt gaaggtcttc taaaaggcca tacaagcatc gttatcgcac ataggctttc
      181 aaccattcgc gatgctaata ggatcatcgt acttggacaa ggtcgtgtgg cagagatagg
      241 cacacatgat gagttgattg ccctgcatgg tttgtattgg aatctctata ggcttcaaaa
      301 caaggaaatg gaataaatga tgccattttc agaagagaag gagcctttta taatcggcca
      361 agatgatcgg gttacggctg tttacaagcc atgcggctgg cattccgtat gtcaaaagaa
      421 aagcgatcac tctatagtat cttggctata tgatcaggga atacctaggc ttagagagtc
      481 tcaaatggat caagagctcg gtcttatcta taggctagat cagatgacat ctggaattct
      541 gctatttgca gcaaatccgc agagtctcga ggagcttcgg caagcacaga aagaacttgc
      601 cattgtgaaa cgctatattc taatctctac attctcagaa tgtgagcttt taggatcttt
      661 gcctggaaca ttgaaagaaa agcaatcatg tttgattgaa gagctttttt ctaaaagaga
      721 agcatatatc gaaagctatt ttcgtccata tggtccaaaa ggcgcttcgg tgtcctgtat
      781 tgcggcagaa tttgcttcca aatccaaaaa gccaattaca aaagagatat atgttagtaa
      841 atttattgca gcctatagga tgatagaaga aagcaatgca agactgcgac taccaaatga
      901 ggcagtgtgc ctcgaggttg aaattcggaa gggttttcgc catcaaattc gggctcattg
      961 cgcatggata ggcttgccta ttcttggaga tatgctatat ggcggacaag gctccaatag
     1021 attgtggctc gaggcttttt cggtatgtct tcctcaggct gatcgatgcc gcaatgaatg
     1081 gaagctatat aatggaatcc aagatattgc ccaggtttca tctgaatgaa gattcctgtt
     1141 ctatatatta ttccacaatg atttttctaa cagcaaattg cgaacaagtc tggacacaat
     1201 ccaaaaccca ttcccatatt cgatgcttga tgataaaata ataaaacatc cttcaaggaa
     1261 tgatattact ttttagagag gtttgaaatg gcgtacagag tgacggaagc ttgcacgaat
     1321 tgtgggtcat gcgaaagcga atgcccggtc agcgcaatta gcgaaaagga caatgctcga
     1381 tttattgacc cagagacatg cattgattgt ggagcatgtg ccgcggcctg tccgacagaa
     1441 gccattattg ctggctaagg tccaagacca taagaggggc tgcccaacga gttgggcagc
     1501 ccctcttatt ttcatgtagg atcaactcat taccttgagg agattcttat tatatttata
     1561 ttaagataag ccttttctct tgcaaaaatc ctctaacact tcctcgagat taggcaatcc
     1621 tatctcttga agttcgtaat cgactcgcac ggttggcttt tttatgtgaa taattcggtt
     1681 caaatcaata ggagttgcta tgatgacagt gtcgcagtcg gttctattta tggtatcttc
     1741 aagatctcgc ttctgcatat cgctatatcc cattgctgga aggagagtcc ctatatcggg
     1801 ataagtattg aatgtctgtg ttaatccccc actgaggaat ggccggggat ctatgagctg
     1861 ggaggcttgg aattttcgag ctgctaccac accagctcca aatttcatca tgccatgtgt
     1921 aagagtgggc ccatcttcca cacacaatac tcgcttgcct cggatgagct cggggcgatc
     1981 cacaatgata gggctagccg cttcgatgac agtggcagaa ggatttgtcc gtgctatatt
     2041 ttctctcact atctggatct gctcgggtga gcttgaatcg attttattga ttattatgac
     2101 gtgagctgtt cttagacaga cttcgccagg gtaaaaggat acttcattgc cagcgcgcag
     2161 cggatctgcg accgttattt gaagatctgg aatatagaac gaaaagtcat tatttccgcc
     2221 atcccagaga attacgtctg cttctttctc cgcctggcga agaatggcct cgtagtccac
     2281 tcctgcatag atgacattgc cacgagatat gtgcggctca tattcctcca tctcttcaat
     2341 agtacaccta taatgggcga gatcttctat agttgcaaaa cgctggactt tctgttgtac
     2401 aagatcccca taaggcatgg gatggcgaat cgcaaccact ttaagcccgc gcttcataag
     2461 catttggacg atttttcgcg aagtctgact tttgcctgag ccggtgcgta ctgcacaaac
     2521 cgatatgacg ggcttgctcg attttagttg ggtttcctta gggccaagca gcgcaaagga
     2581 agcaccggcc gaattcacaa tcgccgacat tttcatgaca tgagagtagg gcacatcgga
     2641 ataagagaat acgcattcat ctacatcata ttttctaatt agatctgaaa gctcgctttc
     2701 ggaataaatg ggtatccctt ctgggtaaag tcgtcctgca agagcagctg gatatcttct
     2761 tccgtcgata tttggaatct gcgcagccgt aaaagctaca acctcggttg attcatcttc
     2821 tcgatatcga gcattaaagt tgtgaaaatc ccttcctgct gccccgatga ttattatctt
     2881 gcgctttttc atcaaagctc cttccttttg ttttgcctta tgaatgacaa agacatgcat
     2941 tcatttttgc ctattttcgt atcagagtca ataaggtcac attataataa ctttttataa
     3001 tataagcatt aaaatgaata atgatgcgat cttgaagcta ttttattgag aattttgcaa
     3061 gatttgccaa ataataacta atacatatac agtttaagga gggttcgtga cggtcatggc
     3121 aatctttatc aggccgctgt cataatccat aatatcgact tatacttaag agtcagctgc
     3181 atccggtggt ggatccacat gtctcacatt tgaggcaggt accatttctg atgagcgtaa
     3241 ggttaccaca aacaggacag gcatctcctt catacccttt ttgcttggct tccctgatga
     3301 gcataatatc cgtatctttt tttattggaa tgtgtttttt accactctca aaggcgttag
     3361 gaaccttagc ggaaagcgat tgcgtggatg tagcaatgag gtcttcgggc ttgatctgtc
     3421 cgagatcata acgatgaaga taggagattg cgagatcgcg gaaaacaaaa tccaggacac
     3481 ttgtcgtcat ttttagataa tcatgcccgc tgactattcc gtttggctcg aatttcgtaa
     3541 atgtaaaggc gtctatgtat tcttcaagag gtaccccata ttgcaaaccc aaggatatgg
     3601 caattgcaaa agaattcaaa atgctcctga aagcagcacc ttccttatgc atatcgagga
     3661 aaatttcccc aaggctacca tcttcgtact cgccggttct aaggaagatg gaatgacccc
     3721 ctatctttgc tttctgggta tagccctttc ttctatttgg aagagctctc ctgacaccgc
     3781 gaggtgcaac aggaaggcca ggaagttcag gctcgatact cttatgagct aagccggttg
     3841 gaaccatttc cagagaagaa atatcttctg aagtgagttc atcagactgt agagctatga
     3901 tcgaatcggc aatgagattt gatcctggtg ttaaagcggc aagaggttga gaaagttttg
     3961 atccgtctct atatatggcg atggatttca gcatagatct ccaagcgagc aaatatgccc
     4021 cttttatgtc atctatgctc acattattgg gcatgttgat ggtcttggag atggctcccg
     4081 tgatgaatgg ctgcactgct gccatcatgg ctatatgcgc ttgccacgat atgctgcggc
     4141 tgcctttctt tccagagggc gttgccgtgt cgaagacaga taggtgttct gatttaagat
     4201 gaggggcgcc ttccataccc attgttccac acgcaaagat ctcagcttct tttttttgct
     4261 tttcatcata gcctaagcga gatagaaggc tgaatccagg agcattagct tcctctttcg
     4321 atattcccag tttttctaga acatcatagc caacaataga tggagaaaag cagctatcca
     4381 gactgattgc gttttgaaga gacttttcga ttctatcgat tgagtcattt ggaatgccct
     4441 tatcgattag tgactgacgg gagattcccg gtgctccttc gaatgttccc tttcccagag
     4501 catattgtat gatctcctta atttggctat cagaatagcc aagcgcatgg agagcaggag
     4561 ggaccgaggt gttgatgatc ttgaaatacc ctccgccagc aagtttttta aatttgacaa
     4621 gcgcaaaatc gggttcgata cctgtagtat cgcaatccat taggagtccg atagtgccgg
     4681 ttggtgctat ggcggtcacc tgagcattcc taaacccatg cttttctcct agcttcaatg
     4741 cttcatccca acattcgtgc gctgcgttta gaagattagt agggcatgac cgtgaatcta
     4801 ggcccttagg gaaaacactc aatgcctcgt attgctcctt tgcggcatca tatgccgcat
     4861 gcctatgatt tctgatcact ctaagcatag attgtctatt agcttcataa cgagcaaagg
     4921 gcccaaattc tctcgccata atagctgatt gggtataagc ttcaccagta agaattgccg
     4981 atagagcagc tgccagagct cttccattat cggaatcata aggaagaccc ataaccatga
     5041 gcaggcttcc tagattggcg tatcccaatc caagggtccg atagtcatag ctatttctag
     5101 cgatctcctt agagggaaat tgcgccatta caaccgaaat ttcgagtaca agtgtccaaa
     5161 tccgaatcgt atggcgatag gctgcttcat caaatttgca tgtttcgaaa ttataaaaag
     5221 atagcaaatt caaagaagcc agattgcagg ctgtatcatc gaggaacata tactcagagc
     5281 atggattaga cgcgcgaatc tcgccatcgg cggagcaagt atgccattca ttgattgtcg
     5341 tatggaattg cagaccagga tccgcacaaa accacgctga ttctgcaatt tcattccaga
     5401 gatcgcgcgc tttggttaca cggaagggct tccgcgtagt acgggctatc agtttccaat
     5461 ctagatcgtt gataactgca ttgataaaat cgttatcaac tcttacggaa ttattagaag
     5521 attggccgga aacagtgtta taagcttctg attcccagga tgtattgtat tgtggcattc
     5581 gaggagttct accggtctgt gcataatagg tcagcacctg atgaatccat gatgaaggga
     5641 ttcctgcatc gagcgcttgt cttattgcct tacccaagag cagatttcga tcgggcagat
     5701 agctgttttc gtcctgaact cccatgtctt caatcgcaga gatcattgcc tgcgcataat
     5761 gattcaaaag gccactgcct gccgcaagac aggcgacctt atattcctct tcggccttcc
     5821 agtgaacaaa atcttcaaca tctgggtggt ctgcatcgag gataaccatt ttagcggcgc
     5881 gtctagttgt accaccggat ttaatggctg aggcagatct atcggctatt tttagaaacg
     5941 agagaagacc cgatgaaaca ccccctccag aaagcttctc atttgcagct cttaagcgtg
     6001 agaaatttga tcctgtacca gaaccatatt tgaataaacg agcctctttt gttacgaggt
     6061 ccataattcc gccttcattc accaagtcat cctttacatc gagaatgaaa caagcatgag
     6121 gctgaggtcg ctcataggct gattcggatt ttgccatctc attggaatcg ggatctataa
     6181 agtaatgccc ctgggcgggt ccttcgatgc cataaacaga aaataacccc gtattgaacc
     6241 attggggact atttggtgcc gcaatctgat gtgctagcat atagcagatc tcatcataaa
     6301 aggcttgtcg atcttcttcg gaatcaaagt atcccgcttt ttgtccccag tcgcaccatg
     6361 tataggcaag cctatggaat acctgccgag catcgtgttc ggcatcatcg accatatcct
     6421 cttcagagag gattgtctga gagacaggga caaatttcgt ccattcgagt gccttatctt
     6481 tggggactcc ggcttttcga tagtattttt gggctaggat atcgcttgct atagagctcc
     6541 agaatgaagg cactatgaca gaatccgttg aaaacacaat agaaccatcg ggattcttga
     6601 tctcactttt cctcttttcc catttgatct tctcataagg gccatagcct gctctggtaa
     6661 acaagcgctt tattttcata ttctctctcc ttgagctgcg ctatactatc tatactatca
     6721 tagcagtatg atactacaat atatagtggt gctctttctt atcttatttc ttatcatgga
     6781 gacttccgat gcaataatcc ctgtagtgat tttctgtgtt tctctatgtt tggtttgctc
     6841 cagcgcatcg gaaaacactg ctttggggtg acagacaagc ctattcctgc tattgttatg
     6901 ctgaaatgca taaccttccg gatatgatct tagtcttaat ctccaggaaa agattttgct
     6961 taaagatttt gtacaagact ctggccatgc tagccatgat tgccctatgc attctaccat
     7021 tatttggtgc aaatgataca aaacaagaag atatgagcat ttctgatcta gcctgttcgc
     7081 taaaggctac cctggaatat gatcctttag ttcatgccgg aaccattaca aaagaaggtc
     7141 gcagtgtgcg ctttgccatg gatgttccat acgtgcttct cgactggaca atagtaaaag
     7201 aagtgcccgc gccttacgaa tcggctacat ctctgcaaat caaggaggaa tttgcacgga
     7261 cagttcaaga attcttcgaa ataaaatcgc aaatatcatc aagatatttg gtaaaagcca
     7321 ttgtcataga tccagggcat ggtggcaaag atccaggggc tataggagaa ttcgaggact
     7381 tcaagcttca agaaaaagat gtaaatctca gtatcgcgct tagactcgcc gatctattgc
     7441 gccttcgata tcctgaaaag acgattgttc ttacccgctc gagcgacatt tatccaactc
     7501 ttgaggaacg ggtcgaaaaa gcaaataaga tagaattgga agaaactgag gctataatct
     7561 atgtttccat ccatgccaat gcctctttta acaagaatac gaaaggtttt gaggtctggt
     7621 acttaaatcc caattaccga cgtaccgtgg tcgatgaaag aaccgctaaa gagaaaggag
     7681 aggacatcgc tcccatcata aatatcatgc ttgaagaaga atttactacc gagagcataa
     7741 tcctagctca gaaagtatat caaaggatgg acaggataat cggtgataga agccccgcgc
     7801 gagggatccg cgctgaagag tggtttgtag tgcgaaatgc aaaaatgcct tcgattttga
     7861 ttgaagtggg ttttgtgacc aataaggctg aggctcttct tttgtcccaa gctggctact
     7921 tgagacaaat tgcggatgcc atatacaatg gtgtttgtga ctttatcgaa catttcgagc
     7981 aatgatatga atccatctaa agaacaagac cataagcaag gctatattat cgccctggtg
     8041 atttttcttg ccattgtatt catcgtatgg aaagggccaa cgcttcaata taatagcatg
     8101 cgtctctatt tcccagataa gaaaaaagga gaattgaggc tggagaagcg tcctatagcg
     8161 ccaattggga ctctaaaaga aaaagccaag gctactgttg aagagcttct gcttggacct
     8221 ctttccaggg atcttaagat cctgacagat acggatattt ccctgctgcg aatagttgaa
     8281 gggaagaacg ctctgtttat ggatttctca acacaagata ttgcaagttt ctcttcagag
     8341 tatatgacat ttaagagcgc tctggaaaag agtcttaaag agacaatacc aggtactttt
     8401 catatctatt tcttcataaa tggtgttctt gcgcgatgaa ttaaattttg ccgatctctg
     8461 aataaaatcg atgtggctgt gcacaatgct attctctgcg gctccatgca cagcacagca
     8521 caggcaagcc aggaatttaa acaacaatga tgttgatccc tcctttgccg aaagaaatat
     8581 ttcatgataa ctttattctc atgaatgatc ttgagaatat tccaaacaag actcttttgg
     8641 aaaagcttta caatcaacat cggactggct atgaggaagc tctagccaag acagtcaatg
     8701 acatctccaa tcttttgcag gacaaaggaa tacacccaac tataaagagc agggtcaaag
     8761 atttcgaatc ctatttttcg aaaaaaataa gattccttaa gaaagcttgg gatgagcata
     8821 aagacccctt gcccataaat gatgttcttg ctatgcgaat tatttgtcca tttttgcgag
     8881 atttggaaga gaccgtacat cttcttgacc agcgctatga ggttgtcgaa atcgagcgca
     8941 aaggacagga ccgttcggtt cgggaattcg ggtatgaatc aatccatgtg ttgattcgca
     9001 ttcccgatga gcttgttccg ctctgtgaag gcttggaaag atatgtaatg gagattcagt
     9061 tgagaaccat tctacaggag gcgtgggcgg aggtagagca tgaaattgtc tacaaggcag
     9121 aattcacccc tctcgatgag cctttgaaaa ggaaactagc cgccctaaac gctacactta
     9181 ccctgtccga cataattttt caggaaatac tggattatca aaaaagactg agcgatgcgc
     9241 ttaaaaagcg tcgccaggga ttctatggaa aaatagaaca gaaaacagcc gaaatctttg
     9301 gtatatctga agaaataaca gaacctattc cctcattttc tggccagatg atggaacaaa
     9361 gagagacagc ttatccaaat gaaaaccagg tctcagcttc gaatcgatcg aatggccttg
     9421 acatggatat gctgcttctc tctgctctca acgcacataa tgcctcagat ttcaatagtg
     9481 ccatatcaat atataccaag attctcgata aaaagcccga ggcatcgctt gcagcagtgg
     9541 tgtacaaaca tcgaggcatg gcctattttg cacaatccga atatgaacaa gctctagccg
     9601 attttaccag ctgtctcatt ctcgatccca actgctataa ggctctatac taccgcggag
     9661 ttgtaaagag cattcaaaag gagctgccac aagcaataga agatttttct aaggctcttg
     9721 agatacaccc atatcatttc ttttcccgat atagaagagc tctatgttgg tggcagcttg
     9781 gcgattatgc gcaagctcat gccgattgcg aacttgcatt gcgcataatg cccgaaaaca
     9841 agctagctca ggaattacag aagaaaatca tggagcatct tgctaaagaa gacatataaa
     9901 ctctcattga atgcaagttt gccctctaat agcatctcaa ttatagcctt gcttattgac
     9961 atgtattgga aattctgata ggttcactgc cgttcggcac tctcgccccg agagaggccc
    10021 tatgtcggaa tgtccccatc gtctagaggt taggacagcg ggttttcaac ccgccaacag
    10081 gggttcgatt ccccttgggg atatagatgg agccatcata gattcatggt ggctttttta
    10141 ttatatacca gattttagcg catctgcgaa atcaaagcat tttctttcag aattatcgac
    10201 agccgattca attagattac ctaaagcaag cgattcaaaa tggctcctca gctcgccaaa
    10261 atcaggctta agaattgggt gcttagggct aaataggaca cagcagtctt cataaggcaa
    10321 gattgagata tcatatgtgc ctatttgtcg agcgatagca atgatccctt ctttatcgat
    10381 tccgatcaga ggtctcaaaa caggcaggga agttttgctc tgagataagc gcatgttttc
    10441 cgctgtttgg cttgcaactt gcccaaggct ttcaccggtt acgacggagt ttgcgtttat
    10501 gcgatgggca attatatcgg ctgcttgcat cattgccatc cgaagcatca gtgtttttgc
    10561 ctcctcataa gatgctttag cgatagcaag ctgaatatcc gtaaatggta caatccatag
    10621 attcatgcct cctgtccaaa ttgagatgcg cgcggcaagg cgttcgactt tttgctgggc
    10681 ctcgggagag gtataaggat agctatggaa ataaatgctc tcgagtgcaa gaccccgaca
    10741 agccatcatg tatcctgcta ccggtgaatc aattcctcca gagagaagca aaaccccctt
    10801 gccttggctc cctacaggta atcctctagg tcctgtcatg gtcctggaat agacataggc
    10861 ttgctctcgg atttcaatcc tgattataaa atctgggtta tgaacatcta cagagagctt
    10921 cggatattcg gagagcaggc tattaccggc gacaacgcat atcttgtagg aatccaaagg
    10981 gaaactcttg tctgaacgtc tggcttctat tttgaaactg tgcgagccag ccgaaatctc
    11041 tgtctttgca cattcgactg tggcctggca gatctcatct atatcctttt ttatcctaat
    11101 ggcttttgca acaccattta tccccgggca atgctgaagc acgaatagag ccaattccga
    11161 atctttctct tccacagtca ggaaaaacct gcctggatat tcctcgaaaa taaatggaat
    11221 tcccttgagc ctcttggtta tctgttcttt caagcggcgc gtaaattcgc gtctgttgcc
    11281 caattttagt tcgatttcgc ccggtttaat caggaaaaga tcatgcatag cccctgtatt
    11341 atcaggtttt gagccttcga tacaagacct cagcctgttc cagaaaagaa tcaatgtctt
    11401 gtggggttgt caatggtccg gtagagacac gaattgatga aaaagctatg tcatcaggca
    11461 ctagcatggc cttcaagact ctccgttctg caagatggat gttcgacata catgctgagc
    11521 cagtagagac agcaatgtgg caatccgaca atgctcgcac catagtttct ccacctaaac
    11581 cagggaaagc cattgatatt atcgaaggga caaagccctt gtcctgggga attcgttcgg
    11641 gaagtgtgag tgtattggga atgctggata tgccatctaa gagtcgcttc tcaagtgcaa
    11701 tagctttctc ttggttttcc tgcatcgata gtgccgcttt ttcagcggca agagaaaagg
    11761 caagtgcgcc aaacagattc tcggtacctg gcctcatgcc agactcttgt cctccgccgc
    11821 ggataaaagc ttccagaggt cgagcaatcc aaagtccgcc gactcctttt ggtcctcgaa
    11881 ttttatgtgc tgatattgca aagctatccg catggtcctc aagatctcta attgataatt
    11941 ttccaagcgc ttgaacagca tctacatgga gccagggctt ttttatcttc ttttggagaa
    12001 cagtctcctt gatactctcc gctattgcct tgatattttg aatagctcct gtttcattgt
    12061 tgacattcat gatgcagata agggctgtct ctttgcgaat tctagattcg atttgttcgg
    12121 cttcaatatg tcctttcgaa tcaggatcga tccttgaaac ctgaagtcct agtctctcca
    12181 aaatgcgcat ttgctcttca attgcagagt gttcgatcga agatatgata gcgtgcatgg
    12241 gtttttctgc agaactagct cgtttcagct tttcaaggat tgcaaagata ggaatgtgat
    12301 ctgcttcagt tccaccgctt gtaaaaataa ggctcccttt tgtaacaccc atggagcgca
    12361 tgactctcaa acgcgattct tcgagagctt gccgagcctc ttttccaagc caatacttgc
    12421 ttgaagggtt tccatatgct ttaagactga attctagtgc aatgcgcatt atgtcaggat
    12481 cggggggtgc tgttgcagcc caatcaagat agatgtcttt catccagaac ttatgctcct
    12541 aatgccattt tccattctgt ctcaatgaat ccgatgagat gttctctgga atctattacc
    12601 ataaaaggga agatgacgtt ttctttaaat ctctgggtaa gctcattctt aatcgcgttt
    12661 tttgattcaa gtggaaccag atcgatataa agatatttat aggcaaaatg attcacatcg
    12721 aggaaggtta atgctctctt acaaaagata caggtggata gcgcatatac cataatttca
    12781 tgatcttttc gcatgccttc ttttttcata atagaaatat tttctagatt cattaaggtt
    12841 ctccagattt caagtattat atatccgaaa tggtattttt tgatatagag acagtaggta
    12901 atccttggaa ggaaatcaaa atctgttgat aaaagccttt gtttcgtata cagttggcct
    12961 atgattgagc ccgccttcat tcgcaatttc tgtattattg cgcatataga tcatgggaaa
    13021 agcactcttg ccgatagatt tatcgaaaag gcccacctta tcgattcaag gaaattccag
    13081 gatcagatgc tcgataatat ggatatcgaa cgagagagag gcataacgat taagagccag
    13141 gccatcagtc ttccttatgt agctaaaaat ggaaagactt atatgcttaa tcttgtcgat
    13201 acccctggac atgtcgattt ctcttatgaa gtctctaggg cgatcgccgc atgtgaggga
    13261 gctgtcctgg tcatcgatgc tacacaaggc gtcgaggcgc agacgctttc gaatatgtac
    13321 atggctttag aacatgattt ggagataatt ccggtcataa acaagattga tatggctagt
    13381 gccgatatcg aaggcgtaag aggtcagatc acaaaagatc taggtttgga aggagaaagc
    13441 gctcttgccg tatccgccaa attgggaatc ggcattgatg agctctgcga ggcgattgta
    13501 gagaggattc catcgcccaa gggtggagca gaaaatcctc ttcaggctct catatttgat
    13561 tcgcattacg atccatatag aggggctatc gtacacctta ggctaaaaga aggttctata
    13621 aagaaaggta tgcatatcag gctcatgtct acaggggcgg tccatgaagt agaggaggtg
    13681 gggcatttca gaatggcgct ttctccagca atggagctct ctgcaggcga ggtgggttac
    13741 tgcattgcag gtataaaaac cgtaaaagaa gtacgcgttg gtgatacttt gacagatcat
    13801 gaaaatcctt gtaatactgc attgcctgga tttagagaag taaagccagt tgtattcagc
    13861 tcaatttatc ctatggattc ttcagattat gaggaattga gagatagtct cgaaaagctc
    13921 gtactaaacg atgcatcgct tatatttgaa aaagaccatt cacttgccct gggatttggc
    13981 tttcgatgtg gatttctagg actgttacat cttgagattg ttcaagagag acttgagcgc
    14041 gaatatgatc aatcaatcat tatgactgcg ccgtcagttc gatataaggt gattctcaaa
    14101 gatggctcaa ccctatatat cgacaatcca agcgactatc ctgatccctc gcgcattgag
    14161 aacgtagagg agccctatat aaaagcggag ataattacac ccgtagacta tataggcaat
    14221 ataatttctc tctgcgtaag caaacgtgga agtcagcaat cttttctgca tttggatgaa
    14281 aggcgcgtag aactcatatt tgaaatgcct cttgcggagg tcctctttga tttctacgat
    14341 aaactcaagt ctatatcgcg cggatatgcc agtttcgatt atgtagtgac aggttatagg
    14401 cctacagacc ttgtaaaatt ggacttctta ataaatggag agcccgttga tgcactttcg
    14461 atgcttgttt ataggccaaa tgcatacgat cgggctaggg ttgtctgcga gcgcttgcgc
    14521 aacaatattc cacgccaaca attcaagatt cctattcagg gatccatcgg aagtcagata
    14581 atagctagag agacagtcaa tcctgttcga aaagatgttc ttgctaaatg ctatggaggg
    14641 gatataacac gcaaaagaaa actgctagag aagcaaaaag agggcaagaa gaaactaaag
    14701 atcatcggaa atgtcgagct ggggaaagaa gtatttttgt ctgtgttgcg ctctagtgag
    14761 gaagaggata gatgaaaggg gctctcagta aaaatggaca gccccctttt ttattccttg
    14821 tgctgaaata cctttagtta attagacaca aagagcttcg caaaagtagc attgaagtta
    14881 tctttaaatt tatccgatgt tagaatggaa tttcgaatat ggttctccat ggcggtctgg
    14941 aaaaaatagg gataagagaa ttttgtataa gaggtttcct gatcgcttgc ctctctgtct
    15001 tccgtgacct tcatgaccac taccgaagca tctaagagca cgggttcaga tactgcatct
    15061 ttgggtgttg agaaaagcgt ggtaaggaaa gtctcgttct ctacagcacc aacaagatcg
    15121 gtatccagcg agcgataagg ctcttgaaaa aggctaactt gctgaccata gaaatagaaa
    15181 cctggattgc cgtaattgag tataaaagga cctgctggct ttacctggag tccgactttt
    15241 ttcgctgcag attcgaagga ttcgttcatg agttcagctt tgaattcatt tgcctttgcc
    15301 agagcccaag attcaagcac actcttttct ctatcaaaaa tatagctttt gacttcgtct
    15361 aggacggatt gatttgagaa atcaggagcc ttcgcttcgc tgtttattcg gtaaaaagcc
    15421 caggtgttgc cggtttgttt gtagaccggc gaaatttcac ccttcttgag gctcatgaga
    15481 ttctctgcgt ctttttcatc aaggaattcg cctttcaaat cgtagaaagt ctttgtaccc
    15541 atatcgccac ccttatccgc atagacatcc tttgagtggc tttttgcggc gtcttcaaaa
    15601 gaaaggctgt tttccttgat ttgggatagg actttctttg cgtctttttc cgatgtgcta
    15661 atggtaatcc tggacagacc aatcgtcctg aatagagact cgtgagactt agcccattcg
    15721 agcgtttcct ccacagggaa gtcggcatag gagatgttga catactgaat tgagcgttgt
    15781 ggctttgcca tcgatgctac aaattcgatt tccgcagtgg aaggggaagc agacataagg
    15841 tcgttgtagt agttttggat tagcatatct tcccggatac tatttctaat agaaaggcgg
    15901 gtcgcagcag gagtgctgtt gtatttttcc aatgagaact tgccgtctac ctggaattgg
    15961 gaattcttag aaatttcttc atcaattgtc tcttcgctta ccttcatgcc agatcgcttt
    16021 gccgaatcta ggatagccgt gcggatcact gtgttttgaa acgcgagacg ccacacttga
    16081 taggcgtaga gctgcgattg cgtttcatca agtccctgct gtcgaaggta gtcgtttatc
    16141 tgcgacacct gctgggcaaa ataagatcct gaggtatatt gtattggatt gccattccac
    16201 gatccaaatg aaggaagctt agcgccagca cctgtacctg agctaatgct tggaataagc
    16261 acgaatgcga taatagtaag cacaagaatg attatcgtgc caatatacac aaaggggttc
    16321 tttatggttt tctttagagt ggtcttggtt tgtttattat cgcccttttt atcgccggat
    16381 ttcttaatgc ctgtttttgc ttgtttttca ttctcttcgg atgccattcg ttgtcctttc
    16441 atccataatc ctttaaaaaa gctgcaataa attatcatga ggctatggaa tagtcaacga
    16501 gagccctggc catttctgct tggagagata agcgatagcc tctttctcag atagaaatct
    16561 cttccatgga ttcggatcat cgatattttc atcggcaata gctatttcaa agggagatat
    16621 tcgcgcctta taatccattt tggaggcgcc gggaacccaa aagcctaggt aataatagcg
    16681 cttatcttcg gaatcattgt cagctgcttg gctttttgca tcatcacgtc caagtttgtg
    16741 ctgtattcta ttctcataga aatgagaaac cgattctccc tttatttcct tattcaacaa
    16801 aaggtgtttt gccttttttg cgaaactgct caggtaggat tcagcaagga ttgagaaaaa
    16861 gcctaagctc cgtcctcttg catcaggttc aaaggagaaa taaacagaag acaatccatc
    16921 atccagagga tctacatggc ttactgcaag cagtttcctg cctttactat tatctcgata
    16981 ctcaaatatc agactccatg gttccaagag gtttttataa gagtcttctt tgaggtcttt
    17041 gagaggtatg gagtgtttcc aaatagaata gcgctgccat agtctgaaat gctcctcatc
    17101 gaattccggt ggatttgaaa taatgatgat atcattattt ttttttagaa tcctattcaa
    17161 agaaagcgta agctgaattg actcagcctt gattcttagg ggaatacaga gcttgcagcc
    17221 agggcactcc attcgataga tttctttgcc atgtcttctc catccattcg atatcaaagc
    17281 actatataat cgagagtcaa cttcatcctg aaaaatcagc aagttcatca tagtggaatg
    17341 attattggat aggtaagggc atgttccttt gacttgaaaa taaaggacgt atctattctc
    17401 cgaagtcatg tctatttcct taaatcgcgc tacattaatc ttaaggcatt aaatctgcat
    17461 aaaaaagctc tccagtagct tgattgttgg tcttaggctg tataatttaa ctatatgagc
    17521 attattacca agacaggcga tgagggcaca acaggtctct ggtctggtga gagaataggc
    17581 aaggattcgc caagagtaga agcatatggt acaatcgacg aactctcatc agccctcggg
    17641 atggcacgtc atctctgcat gcaggacaat gttctttgcg caatcgagta tattcagcgc
    17701 cttctattca gagtagccgg ggaattggca tctatgagca tgccttttga taatcctatc
    17761 aacgaagatg acgaaaagac aattgaagac aagaccaatg aacttgaaat gcgcattccc
    17821 ataaggagct tcgtcatgcc aggaatgacc gggggttcgg cggcactaga tctggcgagg
    17881 acaattgcac gcagagcgga gcgtcgcgta gtcgctcttt ctagaataga gcatgtttcg
    17941 cctgttttgc tgcgcatact caataggttg tctgattata tatacatgct cgctcgtgag
    18001 gaagaatcga aacaaggaaa gctgaccttt atctaagatt tcttggacag agcgctttgc
    18061 ttgctgattc cataactcaa gcggagaagc gagaattaag gaggtattcc aaatgaggct
    18121 ctattccaag gcgcagatag gtttggttat tgttgtctcc gtgactttga cagctctttc
    18181 aattgctggc atagtatttg ttcttgatag atctgatcaa aaccaatcaa ctatggctgt
    18241 cgcttctaaa atagctcaag acggcgatga agcggtttac atcccagaaa taactagcct
    18301 aggtattcag gatgataaga ccgcaagtct ttctctgcag gtggcgaatt caaccctgag
    18361 catgagcagt tatagtgcgg atgagatcga gaatatcaca atctatgaaa agtacaacga
    18421 atctgtggtc aacatcaaca ccgaagtgct tggtataaac tggtttctgg aacctgtgcc
    18481 tcagagtggc ggatctggtt ccgggagcat catcgatgag cagggttaca ttctcaccaa
    18541 taatcatgtg gtagaaggag catataagct ctatgtgagc cttgcagatg gcagtcgtta
    18601 tgaagcaaag ctaattggta cagaccccga gagcgacttg gccgtgataa agatttctcc
    18661 tgaaccagga aaaaagctga agcctattcc ttttggaaca tcgaaagatc ttaaggtagg
    18721 tcagaaggta ttagcaatag gcaacccatt cggtctcgaa aggacattga cagctggaat
    18781 tatctctggc ctagggcgtc ctatccagga aagcgagaca actattcttc agaatatgat
    18841 ccaaaccgat gcatctatca accctggcaa ctccggaggt ccactattca atatgaaagg
    18901 agagatgatc ggaataaata ccatgatcta ttcgccatcc ggaggatcgg taggtatagg
    18961 ttttgcaatt ccagtagata cagcagttcg cattgctcct cagctcatca agaacggcca
    19021 agtcaagaga ggttggatcg agatgcaggt aatccaactt ttcccggatc ttgtcaatta
    19081 tctacgccag agtggaaaag ccgcacctgt agagactggg cttctcgtgt cgacagtgac
    19141 ttctggtggc aatgcagaaa aagctggcct caaaggaggc acaaccgcag tgcgctatag
    19201 atcgacagta ttctacatag gcggagatat tatcgtgagt atagacggca aacctgtcac
    19261 caaaattgca gaactatatg cagcgctgga agatacaagt cctgggcagc gcgttaatgt
    19321 cgaattctat cgtggttcga aaaggatgag cactgtagtt attcttgcgg agcgcaggag
    19381 gtgaagccct ttaaggttgt gtcccccttt gagccagcag gcgatcaagc tcaagcaatc
    19441 gagactattt cgagcgccat taagaggggt gagaagtatt tgacactcaa gggggtaaca
    19501 ggttctggaa agacatatac tatggcaaaa gtcatagaag ctattcagaa acctgccctt
    19561 gtgatttcac acaacaaaac cttgtctgcc caactatacc gcgagttcaa ggaatttttt
    19621 ccagagaatg ccgttgagta ttttgtctcc tattatgact attatcagcc agaagcctat
    19681 gtaccttcta aggacctcta tatcgaaaaa gactctgata tcaatgaaga gattgatcga
    19741 atgagacttt ctgcaaccgc ctcgcttatg gagcgcagag atgtagtgat tgtcgcgacc
    19801 gtctcttgca tatatggatt gggttcgcca gatctttatc gggagatgag attgtatcta
    19861 gacaaaggcc aaatagtcga tatagatgaa atcaagcgga atctcattag cctccaatat
    19921 gaacgaaacg atatggtatt agagcgcggt cgattcagga ttcgtgggga tgtcctggag
    19981 atttttcccg cctatctgga ggaggcttat cgaatcgaac tggattttga tactattgtg
    20041 cggatacgtc gcctagatcc gcttactggt gaggcctcga atgagttaga ggaagccata
    20101 atctatccgg caaagcattt cgttatgcca gaagatttgg tacagaaggc ggtagatagc
    20161 ataagaaatg aattagagga gcgataccaa ttcttcaatg ctcatggcaa gtatctcgag
    20221 gcagaacgca taaaatcgcg aacagaatac gatatagaga tgcttagcga aatgggatat
    20281 tgcagtggta tagagaatta ttctgcgccg ctgtcaaacc gcaagccagg agaaagacct
    20341 ggagtattgc tcgattactt tccgaaggat tttatttgtt ttatcgatga gtcacatgtg
    20401 acgctgcctc agcttggagc tatgtataat ggcgatcata gcagaaaatt atcgcttgtc
    20461 gattatggct ttcgattacc ctgtgcactt gataatagac ccctaagaat cgatgaattt
    20521 gagtcgatag tagcgcagac aatctatgtc tccgccacac caggtgagac cgaacttcga
    20581 cgatccaaga ctctggcaga gcaggtgatt cgtcctacag ggcttgtgga tccggagatt
    20641 cttgttcatc ctactgaagg ccagatggaa gacatctatg cgcgagtgcg tgaaagagcc
    20701 aggcaaggcg aaagaagtct catcataaca ctgacaaaga aaatggccga agagttgaca
    20761 gattatctga gtgaacttgg tcttcgtgtt cgctatatac actcagaggt agagactatc
    20821 gagcgtgttg agattctaac acagttgcgt gcgggagaat tcgatgtact ggtaggaata
    20881 aaccttttaa gagaaggcat agatcttcca gaagtatcat ttattgcaat tctcgacgcc
    20941 gataaaattg gtttcttgcg ttcagagaca agtcttattc agatcatagg aagagcagcg
    21001 cgcaacgcgg caggccttgt agtaatgtat gccgatgtca tgagcccagc tatgaaaaat
    21061 gccattatgg agaccgaacg cagaagaaaa atgcagcaag cttataacct tgaacatgga
    21121 ataactccta ggacaattag caaaaaggtc caggatatac tcgagcgcca taagcaggag
    21181 agaatagagg cttctcatgg cgaaatcgag atgcttaggc gcacccataa cttgctggtt
    21241 ccagagcaaa gaagatcctt aataaaagag cttgagaagc ttatgttcga acatgcaaag
    21301 aatttggaat tcgaacaagc cgctttaata cgcgatgaga tttcaaaatt aaaagaataa
    21361 caaaaggatt agttatgtcg aataaccata atcaagagga tttctatgtg atcgatgcac
    21421 actgcgatac gctcatggct atcgtaggtc gcagtatgaa taaggaagag agagatccac
    21481 ggaatttttt tgcagataat aattcggtgc atttggactt gccaaagctt tcaagaggtc
    21541 atgtggcatg ccaggtaatg gctatctttc ttgaggattc gcagctttct gaggctacaa
    21601 gggaagccat ggccatgata gattcgctcg agtctctctt aagattcgac cccagagaat
    21661 tctttcttgc gaggaatagc gcggacatat atagggctct ggagacaaaa agtgtatgtg
    21721 ctttgctctc gatagagggg gcagaagcat tgggcgattc attggagagc ttagaaatat
    21781 tctatcagcg cggagttagg gctattggtt tgacctggaa taggcgcaat gcttttggtc
    21841 gaggcctaaa agccgaagga agcgatggtc ttaccaatct cggcaaggag cttgtcgaga
    21901 agatggagga aatccatgtt atcatcgatg tggcacatct aggcgaagaa ggcttctggg
    21961 atgttgttga tatatccact ggtccattta ttgcttctca tgcgaatgct cgggatattc
    22021 tcgatattcc gaggaatttg agcgattccc aaattagagc aattgctgat catggtgggg
    22081 ctattggctg tgtgtttgtt cccggttttg tcacaaaaga tcccggagac tgtagcctag
    22141 aggcattctt gcttcatatc gatcatattg tgcgcgttgg gggtatcgaa agctgtgcta
    22201 taggttctga tttcgacgga tttatgccgc aaggcggcaa ggtgattcaa gattcagggg
    22261 agtttccgct tctttatgag gcgctgagaa gccatggtta tgatcatgca gcagctcaga
    22321 aaatactcgg cgggaattgg ctaagaatct ttgatgacgt acttggctta gtatgaagaa
    22381 gatcgcgttg attggcatga tgggctccgg gaagagcgaa attggttctc tccttgctag
    22441 taattatgga attgacttca ttgatatgga tagagctatc gaagagcaca caaagaccac
    22501 aataccaaca atcttccgcg attttggaga agagcatttt agggatttgg agcaaaacca
    22561 gctcgccttg atggctgtga gtttcagttc cctagtgctt tcttgtggcg ggggcatagt
    22621 cttgcgcgac tctaaccgct tgatcctaag agattccttt cttactatct ggttggatgt
    22681 atcgataacc gaactgaagc gaagactcca aaaccaaaga catcgaaggc cgcttcttct
    22741 ttcggatgag tgggaatcac aaatagaaga gatctatcgt cagcgctatg agctctatag
    22801 gagtacagca agaatacaat acacctggaa ggataatcat tcgatggaag agagtgcaat
    22861 cgatatcaaa aggctcatcg atgctgctta taatggtatt tcaggaagaa cttgacacat
    22921 accctgatct attgaaatct caatttcctt tccttgagaa gacattgaaa agtcggatat
    22981 aggcataggt aactcgaaaa agtagtcctg catttcggga atcgtcaaga ccagtttgga
    23041 tgccagagga gttgcactga tgagccggac tattttgact ttcattgtct ttctgttgtt
    23101 gttttttcca aagatctcta tagcgctaaa cgggatatat aggcacattg ctttttccaa
    23161 tgccttatcg atattgctta ccgaattgcg acttacttga aacgtgtttc cttcggaagt
    23221 cattatattg aggagttggc tctcttttga ggccgagatt tctgttattg gaagcacaag
    23281 gccaaggttc ataaatctgg caagccagcc agagcccggt tttttttcaa agtcactgat
    23341 aattcctctg ctgatcattt tgccatcgcg cataacgatg atttcatcgc ccaaatcgat
    23401 agcctcttct acatcgtgtg taacatggat agtagtcaag cgctgttttg agagctgctc
    23461 ccgaataaaa cgtcgcatct ctcttctcag gggtgcatct agcgaagaca gtggctcatc
    23521 cagcaacatg agcaaaggat cggatgcaaa agccctggca aatgcaacgc gctgtcgctc
    23581 gcctcccgat aactcaaagg ggtaacggcc taaaaggtgc tctattttga agttgcgagc
    23641 caaagaaatt accctttctt ctctctttct tttttcttcg cttcgaataa acaggctata
    23701 tccaatatta tcccggactc taagttgttc gaatagtgct aaatcctgga agacaaatcc
    23761 tatatgtcgt ttttctgtgg gcaatctgtc aattctaatg ccattcaacg taatagtgcc
    23821 ctcctttatc atctcgagcc ctgcaatcga gcgaaggaga gttgtttttc cgcatccgga
    23881 aggacctaac aacgcaagaa tttttccttc ctccaaagaa aagtcagcaa aaagcgcaaa
    23941 actatcgcgt tcgataagca aattatttac ttcaagcgac atcgactgct ttctccttta
    24001 gcatgaatac gatacccgtc atgagcccaa gcacaagacc ggcggcacaa gcttcattga
    24061 atcgatacgc cgcagtcagg cgatagagaa gaagagggag tgtttcaatc tcgctcattc
    24121 ctagcataag tggtacattg acatctcctg cggttatgga gaaagcgaag gctgcagcag
    24181 ccgccaccga aggtaagatc gtccgcagtt ctatcgtcat aaaagctctc agaggtgaag
    24241 ctccaagtgt tttggcggct tcatgaattc gaaaatctat ggcacggagc gacgcgccca
    24301 ccgttctact cacaaatggc catgcaataa gagcttgagc agcgatgaga gcataagtcg
    24361 ctatatggtt tgcagatata aaaagccatc catatgcaag aactgcgggc gatacagcca
    24421 ttggaagcca ttgcagcaga tccatcataa gatcaatgag catgcttttc ttccctattc
    24481 tctgagcaag gaatgcgcta aagctcgcaa gaatcccaat agaagttgcc aacaacgcag
    24541 caattccact caatttcaat gtagaaagaa ttgctccggt taatggcgct tgaaatcctg
    24601 taaagagtcg cttgaagtta tcaacgccaa atctctcagg gctattcaat gacgctggta
    24661 acgtaaatgc ttcgatcacg atcgaaaaaa gcggtccgat aaagaaaact atgattaaga
    24721 tctcataaag aacgatcaaa aaccattgtt ttcccttggg ttttgccaat ttgtgcgctg
    24781 ctccaaagtc tctcaccgtc ccacgcgtct tactctgcat tctcgtcaac atccatattc
    24841 ctaaaagcgc gcaagcgctc tcaataatag aaaggactgc tgcaagggcc agatttgaac
    24901 gatatcgcaa agcctggtat atttcgactt cgagtgtcgt tcccgcgcga ccaccaaata
    24961 cgagcactgt agtaaagcta aaaaagcaat ataagaagat taaacctgcc gattggccaa
    25021 ttccaggcag tatccagggc aaaatacctg ttcgaaaggc ttttgatttc cttgctccaa
    25081 gagttctggc agcttcaaaa gaagtattgg gaatgcgcaa caataatgct gaaatctggt
    25141 gcacaacaat aggaaagtta tagaaggcat gtacgataac cagtccccaa aaactgtaaa
    25201 gaattcctct atattcccct ctcgagccaa agactagaga aagagccgtt ggaagtagtc
    25261 cttcttttcc gtaatagcat ataaaggcga gaataattag gatcggagga aaacagaatg
    25321 gtatagcagc caaggacaga agagtcttcc ttccaggaaa tctgaatcgt gccgccagcc
    25381 atgccccagg tagcccaagg cttattgcca gagcagtgct ggcaaaggat tggcgtattg
    25441 taaaccaaag actttttgct atcgtcgatc ctggtatctg cttcaagaat tctagaatga
    25501 tctcaatgcc tcctatattg ccttctatct cgcttagacc aactatggca gcatgcaaga
    25561 atgcaatgag aggggccaat gagaagatca gaataatgca aagcaccaga tatagaaaga
    25621 tcttggctct catggatttc caatgagaag atcggctgct tctgaaggat catggtcgag
    25681 atctctaatt gttgcactca aagccggata tttttgcacg atagagaacg aaggaggcag
    25741 ttcagttttg atgttagcgg gatacatcca ttgtgtttca ggcagcaaag cttggcattt
    25801 tgcggataat agatagtcta taaattcttg tgcaagttct ctcttcttac tggaaatcag
    25861 tacacccgca aattcgatct gtgttggatg cccttccaca aattgaaggg cttgatagcg
    25921 ctctgagttt tcaaaagcct tatggtatgc ggggctagtg gaatagctca aaactaaagg
    25981 ggcttcgcct tttgtaaaga ggccatatcc agtatcccag ctcggtgtca ttgcaagtac
    26041 ggatgga
//
LOCUS       DAOOWY010000018        24814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2019_length_24814_cov_10.856133, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000018 DAOOWY010000000
VERSION     DAOOWY010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 24814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24814
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2019_length_24814_cov_10.856133"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..1509
                     /locus_tag="PK105_02740"
     CDS             367..1509
                     /locus_tag="PK105_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HOU60656.1"
                     /translation="MQRKENLRQLFVLFICIIGVFALVPVFGQSSPNLRTIAGLQPGS
                     VLFLSIKGDVSGALSLWGTDIYTLDSNVAAAAVHAGVIANGQTGIVAVVICDGLSSYS
                     GSLRNGVQSSSWGSYGLSFRFVGLAQASGSLAQATPSAPPATQTPPTQLKPPTPSTPP
                     LPQPPVASRNLYAFDDPYVLKRNGNFKPGTTFYVQVTGKVSGADVWGTDIYTLDSDLC
                     AAAVHAGILSDGQTGIVKVTILPGQQSYVGSTRHNITSMNYGNYGMSYSLEPVTQSYD
                     QVVWMILDPGTADYIPNVTVGKSYVVWVTGMADQRKVWGTDIYTSDSYIANAAVHAGI
                     LKPGESAPVIVHVLEGQPNYEGSTRNGITTKAYGSYERSYTLEPAR"
     gene            1954..3186
                     /locus_tag="PK105_02745"
     CDS             1954..3186
                     /locus_tag="PK105_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU60657.1"
                     /translation="MKRFLLAFMALLTVTAAFGAGKLTLMQNKPEIDAQLKAYAAAWG
                     QKNGATVVIKSIGGTSGGMGPQLKADYAAGDMPDIFAFDGLEAYKEWEGIILDLSNEA
                     WVSKTSVPFKYNGKVYGFPVAIEGWGLAYNADLLAKAGIDPKTLNNFDAYKKAFEKLD
                     SMKKDLGINSVVSMAASIEMGWVTAHHNFNSLLSNGLPYGDLSVTNDLLAGKVDAKRL
                     QEYADWVELLFKYSDKAILLTGNYDAQVGAFATGKAVFLHQGNWVDPNLKDANATFKM
                     AFAPHGSMKKVTDGIFVSAPSYYAINKESKNIALAKKFLNDLVVTPEGNNYMVKEAGM
                     IPAFSNITLGPEGQLSKSVQQWSAAGKVYSWNQYYFSGDFRDKVLTPIYNQFAAGSIS
                     KAQFIDLMTKAFKDNAKK"
     gene            3313..4233
                     /locus_tag="PK105_02750"
     CDS             3313..4233
                     /locus_tag="PK105_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU60658.1"
                     /translation="MTKKMESALVFWVFLLPVLVAFIMVMVIPFILGSYYAFTNWTSS
                     ARVDSVLRFVGFENFINSLKDPSFLYSFGITFLYTILNMIVINIVAFVLALLVTGDLK
                     FKNVYRVGFFLPNLIGGIILGYIWQFIFNNALPSLGSIISAFDFLSDPANLMLSKPNT
                     AIAAMIIVGTWQYAGYIMMIYVAAIENIPQELIEASRMDGATPWIRLKSITIPLCAQA
                     FTVTMFLTLVNSFKQFDVNVSLTSGGPSTMLMGQPIFGTELLALNIYNTAFISNKLSE
                     AQARAFIFFLILAIISIIQVNVNKKKEIEL"
     gene            4230..5096
                     /locus_tag="PK105_02755"
     CDS             4230..5096
                     /locus_tag="PK105_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU60659.1"
                     /translation="MKSSKIRRPSSFLAEILTIILFVLFLFPFFLVLINSAKTSFEVT
                     QYPLAWPKKWETIVDNIIKIWTSESVCYPSSLLSSAIITAVSLILINVFSAMAGWALV
                     RTKTKVSSFIFFIFVASMVIPFQIVMFPLLSWFRTVTVATGIRLLRTYQGIILSYVGF
                     GAPLSIFMFHGFIKSIPLELEEAATIDGCHRHQIFFRIIFPILKPIQATVLVLNGIWI
                     WNDYLLPLLVLGKGNDVMTVPLAVSNFAGAFVKQWDLILTAILMAMIPVIVFFLFAQK
                     YIVKGMVAGAIK"
     gene            complement(5123..6979)
                     /locus_tag="PK105_02760"
     CDS             complement(5123..6979)
                     /locus_tag="PK105_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOU60660.1"
                     /translation="MIKTIDGDERKVRNKDLAMVHAGPIHSFPIAQHSKEVNEALDLL
                     KEENPEGVPIVSWNEFAELAWSKVEAKHIVIAWHMLLENPAIEMLDNGFRIRSKEEQQ
                     KLIEKERKKKEITETRAAFVEAFGLAWKKNDPSFVDKDQNFKPFVDELSRFARGLAVD
                     SPIARDLGIRLIPQAVHEALLATGFWEPFINPWPDRNGCILSMPSEPKNIRERSELTL
                     DRLDLRYLSSFAIDNPWSKDPDDAISIEGNRVWIHVSDPVSAIAPISTEDEDACARAS
                     TLYLPEKTIPMLHHSLIEAFGLGILPESKALSFGITLQKNGKIDQISIVPSIIKVARL
                     TYEEADVLLETNEVLQNLNEIVEARKKLRQDQGSVDIVFPEVSIRAIDGNVEFLPVPA
                     TRSARLVQELMILAGEAAARWAYAAGLAFPYVSQEPPISAESAGCGAGLSESLAENYA
                     RRKVMRTAIVSSTCGAHAGLGLSFYSQVTSPLRRYQDLLAHYQIHAELARQKSSNILE
                     EGIPREAQIGLSREMLDQRLYLYGLQAAKNRQAERDSRLHWAMVYLSKHRDWKGQAIV
                     LEVDRGHAHIYIPSFGIEMLIHISRNVKPDTNITIALRRVSIPELTATFDIV"
     gene            7210..7458
                     /locus_tag="PK105_02765"
     CDS             7210..7458
                     /locus_tag="PK105_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60661.1"
                     /translation="MTALLVGIGLIVLAVLMVIPGILGWWSDVLAFLRGALPVIAFLI
                     GALAVFIGIADIKDKIEEKKEEEKEKAEEKNKSENSEN"
     gene            7588..8016
                     /gene="rplM"
                     /locus_tag="PK105_02770"
     CDS             7588..8016
                     /gene="rplM"
                     /locus_tag="PK105_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOU60662.1"
                     /translation="MKTAFFTAATAERKWYVIDAAGKPLGRVAAKVASILRGKNKPTF
                     TPSQETGDYVIIINADKVAITGRKRQNKTYYHHTGYPGGIKDFSFDQLIARNPVSPME
                     IAIKGMLPKGPLGRKLFKNAKVYAGPDHPHLAQNPIAIDI"
     gene            8034..8432
                     /gene="rpsI"
                     /locus_tag="PK105_02775"
     CDS             8034..8432
                     /gene="rpsI"
                     /locus_tag="PK105_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOU60663.1"
                     /translation="MVKNINLGIGTGRRKCAVARVFLREGSGKIFINEKDLKDYFAVD
                     GLIAQVRKPFLVTDSENKFDAYITVVGGGLSGQAGACSHGLARALAQIDPANHISLKA
                     NGLLTRDPRMVERKKYGQRGARRRFQFSKR"
     gene            8439..9299
                     /locus_tag="PK105_02780"
     CDS             8439..9299
                     /locus_tag="PK105_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOU60664.1"
                     /translation="MAKRIDSVRKKASGSLEVAVSGGFVFCFDEVDAQLLDYSLKTHP
                     SDLPFSGKYFLDVHSPAEHSVDALARHSSAVHLIAADSIEFSESAIAGHYGPKSILLF
                     QDESIDEDSLLLLGRLNDLHRARSDALLIVSHNEQASRQLYLKLLKRGYEPEIANTCV
                     RWTCAEHYVDDRRYVRMLFKLHCVKKGQGPTRMQALAWPRIGLFESPQTILADGMAAI
                     PEDDLMEAIKKNIESYQRKPSTFSRLDLNSNSSEKRTFLRSFLRRQGFPYQSIERFLE
                     TLVEKEHKND"
     gene            9383..10036
                     /locus_tag="PK105_02785"
     CDS             9383..10036
                     /locus_tag="PK105_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU60665.1"
                     /translation="MDDDILTIEEVAKYLRVSERTVYDWAQKGEIPSGKIGTVWRFKK
                     SEIERWVNERLSSNRPVSLINSVKIQNIISLDRIIFLDYPSKRDALIKLAENLSTAPQ
                     IKNKEELISEIIRREELMSTAIGKGIAIPHVRLNSVTDLVVSIGISRCDIVDFQTLDD
                     VPVRLLFMIAAAFNQHAYYLQTLSFFSARLKVVELRDGLLNCKTTQEAYDLLIQQDR"
     gene            10122..13001
                     /locus_tag="PK105_02790"
     CDS             10122..13001
                     /locus_tag="PK105_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU60666.1"
                     /translation="MYLKSLEIFGFKSFADRIRIEFSKGISALVGPNGCGKSNIVDSI
                     KWVVGEQSAKSLRAETMEDVIFNGTETRRPLSVAEVAITISNDEGVLPLDVPEIEVKR
                     RIYRSGENEYFINSKPARLKEIRELFWDTGIGKSAYSVMEQGRIDQVLSSKPEDRRYL
                     FEEAAGITKHRVRAREAELKLAKTEEHLRQIEAIIGEVKQSYENLRVQSDKTIKYRQL
                     KDKIFETELDLYLVRLRQYIREQDRVCNLFDQKKKEREALVQRIESMSQSMSDALDML
                     NKLEARLIEMQKLIYGLAVEKNGKERQKALLTERLREIKSIIEQLEARDRAIASKIES
                     LQEEEGDKEAELAGYRIRIREIEANIHTFDENISAAALLLKANEESIRHNVEESKEIA
                     QKTRLLHEKLDTITERIAELVDERLRKSESQIEQKQNLEAQIRQKIKGISISTSSWAN
                     KLQDYTRLAPSLTAQERFRELEGLVIEMRALSSLSSDLEKDFLHYVEIEPGFLNELIA
                     PEGIMTQKRRIDAEILNQRERIGRIEAENQELTLKNTDLAKKIETYRKTLEEARLEKT
                     KIQAQMDAAKEALSVLKREIAGQEAYHREVAGELLDQHRRHEELKEQIELFQEELAEM
                     EQKGRHLTEEMSELDKSISRATEDLGDKRKESSELEQNLHAIQRELEDLHTAKAQRET
                     EIRNLKENFVDLYSRDLLAFESRMYEIRSPVSEIKEKLVSLKASLSSLGSVNLMAPEE
                     FEPVRQRFEFLSGQYDDLVKARADLVRITNEIRTESAKLFVETYNKIKKNFHNIFRRL
                     FGGGRAELRLSDTDHVLESGIEIFAQPPGKRLENISLLSGGERTLTAIALLFATYMVK
                     PSPFCFLDEIDAALDEANIVQLVNLLREFGTMSQFIVITHNKKTVASADTLLGITMEE
                     SGVSKAIAIRIQNENGVVHPVYVLDQAFEEEEVDYEEGRQLEVNR"
     gene            12998..14242
                     /locus_tag="PK105_02795"
     CDS             12998..14242
                     /locus_tag="PK105_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOU60667.1"
                     /translation="MNTEMLDSRLFYLDPWLKEADVVVKTIEPVTDRRSFVFGVMLSG
                     TIFYPEGGGQPADFGFIEDKRVIDVQEIDGEIWHYIELQDENDRDSLKPGSTVHLRLD
                     WPRRLYHMQQHTGQHLLSAVLEEEYGIHTLSFHLGTEYCTIDVSAKDPIELPLMQIET
                     QVEAWIGRDAAVLVHYCPPEDISTFKLRKNPPADEAVIRIVEIQGYDCSPCGGTHLDR
                     TGQVRALKVLSLERYKGHVRMYFASGAHAIELMSKAYDDNRAAATMLGSSVGDISTRL
                     SEVLEKSSSTEKKAKRILVQYAEAEARLASIHAGPTGILQFTLEKEDVDWGTMLCKAA
                     VSLGRPAMASCLNDSTIIIQVPAKARIPGLSKIFSEIMANLGGKGGGGEAFYRASFAS
                     ADIAKQFAIKAREVLERYQTQC"
     gene            complement(14306..15421)
                     /locus_tag="PK105_02800"
     CDS             complement(14306..15421)
                     /locus_tag="PK105_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOU60668.1"
                     /translation="MTNIDETGEFLKSLGFPRLNIHATARHHDFISSSKRILVIGPMG
                     SGKTEFAARIWRDSRVALKKKEVVKRLTSTANVDRRNVFVVRSALDKSRFPDYPEDAL
                     AYRGGYEQCGNRIGAIKDSFGLEVLIAENPEVGTWIIDEAAFFDERLAYLTKDEAESR
                     GLCFIFPTLLLNFRREIFNQTARLLLDTATDVVPLTAYCEHEECLRDSLYTYRYYLVD
                     NQECPALYFDPLIIIGGDRTKHDGREPDYCTRCDDHHYLPAKEYTFFTLKPFGELAAR
                     GNISPLFAELAAFQGKIEESRLYSSILGRCSEGIEREMLMNSLKVPLIAERALIYLYA
                     EQNLLSEEQMRFFIQKLDLDRDYLSKRLADNRRPLAL"
     gene            complement(15443..17047)
                     /locus_tag="PK105_02805"
     CDS             complement(15443..17047)
                     /locus_tag="PK105_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU60669.1"
                     /translation="MNSRFFLVVSLLSIAFSPLAAQMQEREFVTVYGSQIPELDPQKA
                     IFSNEAQIHSAMYEGLFTYDPQTLEPLRAMAQSWERSVDKKLYRFTIREDAIWSDGSP
                     ITAQDFVRSWFRMLDINAEYATFFDIIEGAKQYRMGLDKNPEHVGIKAVSANVLEIAL
                     VRPVAYLTRLLCHHSFSVIHPSMIDKEDWRSAIPYPVNGPYKPVSITETELVLEKNEA
                     YWDSENVSIPMLRMMFTDDDELATSLFNTGYVHWLDGPGNYEKVLLQTAIQVFPIYST
                     HYWFFNCRSEPWNDKRVRRALALLLPWDSIRSSEFYMLPAKTLVLPLPGYSKVKGIEK
                     SDSTEAMKLLEEAGYPKGQGLPEIIIAFADYERPNLIANTLKTEWEKALDVEVTLQPM
                     QASSYYDSIGNRSDAWSFTVAHETWIGDFADPEAFLQMWASDAPLNIAGYNDSAYMDF
                     LIQSYDASDNDRMNILAKAETELLQGAACLPIYHNFAASVIDIDFIQGWYQNALDIHP
                     YKYLRFGTPSISPNVAKASSSYISLR"
     gene            17089..17625
                     /locus_tag="PK105_02810"
     CDS             17089..17625
                     /locus_tag="PK105_02810"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOU60670.1"
                     /translation="MGIEKEFIPERLIVGVLSSDAEAEIAALDAMIGLHGPLCFQSDK
                     EPFLWTRYYCPEMGEDILRSYWAFGELIDPSRLSIIKRATNSIEQRLARNGCRLVNLD
                     PGMLGTARFCLATTKDRSHRIPLSDGIYAELTLIFEHGEFKALPWTYPDWASVPVRNM
                     MTALRAGLLGDLRHLHFL"
     gene            complement(17674..18654)
                     /locus_tag="PK105_02815"
     CDS             complement(17674..18654)
                     /locus_tag="PK105_02815"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOU60671.1"
                     /translation="MINSIKDLNDFSASFEPDLSVKIGSLTMTNPVGVASGILGYGEE
                     YKELVQIDELGALYTKTVTLEPREGNPAPRLVETPMGLINSIGLANPGLECFLREKLP
                     LLRSHCCAIIVNIAGSTEDEYAMLVDRIEEELTLSGPMSKTGVDAYEINISCPNVLKG
                     GMTFGIDPIIVERLTGSLRRRTARPLIAKLSPNVTDITEIARAAEEGGADAISCINTV
                     VGMVIDTDRMKPAIAKGLGGLSGPAIRPIGVAATYRVSHAVSIPVIGVGGITRAADAI
                     QYLLAGAQAVQIGTALFSNPLAPQEILNGITEWMKKHGVLRVHDINSMLK"
     gene            complement(18651..19574)
                     /locus_tag="PK105_02820"
     CDS             complement(18651..19574)
                     /locus_tag="PK105_02820"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60672.1"
                     /translation="MKQFKSAVILNRSIAPNLMHLAFEWSEDLLPPLPGQFFTFLPAM
                     VESSAGIILRRPLAFAGFEPKKHAAKSSGYQSAIAHSIYQIRGPCTRALALQQPGSRI
                     DIIASLGNSFPYPDGDEKAIIAGGGIGIGPMLFLAASLAENGTIAQSYIENNSKSLET
                     SISGKEIQLVFGFRNTLFVPKFKEPTYDIPILNTWEKLLSGAFMATDDGSEGFHGTVL
                     DALECLKKDNKSLEIWHIYGCGPGAMIAELAAFAERNNFPAHLSAEQWMACGIGACQG
                     CVLPARKGGYLRVCADGPVFEADAIDWEACL"
     gene            complement(19651..19881)
                     /locus_tag="PK105_02825"
     CDS             complement(19651..19881)
                     /locus_tag="PK105_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOU60673.1"
                     /translation="MSVTIYTTPSCGYCKMAKEWLQQKGISFTEYNVAADMRRAEEMV
                     RKSGQMGVPVIDIDGKIIVGFNKPEIEKYLHH"
     gene            20051..21577
                     /locus_tag="PK105_02830"
     CDS             20051..21577
                     /locus_tag="PK105_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60674.1"
                     /translation="MKRVRAVLALILHFLSCSMLAWTAETSENPLSGNLSFSASSKKT
                     KLTLFLRHGSDALLNLSIPLSIDKNWLENIAFTGKARLAGYDITAGKLAVKDSASFLI
                     APVLASAQGRSALSISDSGKSSDPIGISIAASRISFLAGKGDPYAFASMQYLCAQEGL
                     LFSIGFGTLLDIESSIDVLDRLDPWASMGIGYTKPSVSIMVRSHLYPDFEKRSENLLD
                     IPWLKKIGMRLDMNIFAGSQKLQGFLYIESGHFLSALGKVASYDAMAQIGYDTDISFL
                     RILKSLNVSLCAFSNQGLVEPVDAKTPSFGTLPDLWTMQYWPDGGKIGLTIESQKAAF
                     SLFRTALNLKTSISGALAINTKSWSNNIALDLMLLRAASSEMFKINGTLQGSHSIGRK
                     LIEIGEEESSESIGNEELGSIEETEDQAASPDHGSSLSPAFDKLLLSGRLKINPFSFL
                     MSVNLPLHNRNSRATSGRIGMSLAKRQLLMELSVSMQVEPESRQLQFKSFSCYAKIPL
                     "
     gene            21676..22335
                     /gene="pncA"
                     /locus_tag="PK105_02835"
     CDS             21676..22335
                     /gene="pncA"
                     /locus_tag="PK105_02835"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOU60675.1"
                     /translation="MDDALIVVDIQNDFCPGGVLAVPEGDSIIPKVNELLSAYSLSIL
                     TQDWHPAMHCSFASSKGLPPFSLDMGTRPPSVLWPDHCVAGSQGADFHPALQSWRARY
                     IFRKGTRKELDSYSAFLENDGVTSTGLAGLLSSLGVDKVIVCGLATDYCVKATALDAR
                     RLGFEVVVVEDAIRGIGASPDDIENAQVQMREVGCVFAQSGDLMASYTISSISPSKGI
                     I"
     gene            22341..23780
                     /locus_tag="PK105_02840"
     CDS             22341..23780
                     /locus_tag="PK105_02840"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOU60676.1"
                     /translation="MYKNALFTDFYELTMAQGYWKREMTMPVVFDYFFRKHPFNGGYS
                     VFAGLATLIEALQEFSFSQDDLEYLSTLGLFEDEFLHFLSSFRFKGTVYAAREGEIIF
                     PQEPLARIEADLISAQIVEGLVLNSLNFQSLIATKTTRIWNASHRGSIMEFGLRRAQG
                     ADGALSASRAAFIGGAMGTSNTLAGKEYGISVLGTMAHSWVMSYPSELDAFNAYAEIY
                     PQNTTFLIDTYNSLESGIINAIEVGKKLKEKGYSFGVRLDSGDIDYLSRAIRARLDEA
                     GFEDAKIVVSNELDEEIIESLVDDGAPIDIWGVGTNLVTGGNEAAFSGVYKLAGIDPE
                     DRLRPVMKFSDNPEKSTNPGVKNLWRLYDEKGAARLDLISCQDEQIQEGIEYIVHHPS
                     ADWRRLKITPARVEPLLFKVMEKGNRTATLPDIRQIQAFMKERIQAFDSTYLRLLNPH
                     IYKVSITDRLYDLKVFLINSFLKHKLSRN"
     gene            complement(23787..24410)
                     /locus_tag="PK105_02845"
     CDS             complement(23787..24410)
                     /locus_tag="PK105_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOU60677.1"
                     /translation="MDANTNRSSKLLSLCVFCGSSMGKRLSYAEAAEKLGRTLAEQGM
                     VLVYGGGDVGLMGIVAEAVMKNGGKAIGIIPRRLYELVDQQEVSELLVVQDMHERKAL
                     MEKKADAFLCMPGGIGTMEELFEVWSWRYIGYHQKPVALLNIEHYYDHLLEMLKHMVE
                     EGFLHKEIYEDLIVSDDIGYLLDALRNKATTSSAAPFLKKPERRNHN"
BASE COUNT         6825 a         5376 c         5763 g         6850 t
ORIGIN      
        1 ccacctaaaa ggaaaatgtg ttagcgcaag cagtaatggc tatcagaagc tgcgaagtct
       61 aatcatgctt tgcaaaatct cttaggaaga gcgccaaaac gatcagaggg gagatcaagc
      121 gtggtatgct agagcaagta ctttcagata ttcccttttc gcgaaaggaa taaattaata
      181 gtgtcacgaa ccattgattt ttttaaattg atttttaaat cttcgctccg attgatttgc
      241 ttttttggga aagacggtgt aggttaaaac agatgattaa attgttttcg caccattgat
      301 atgcacattt tgttcttcgg taggtgccga agcaactctg aaaggagtct gaaaggaggc
      361 gatattatgc aaagaaagga aaaccttaga caattgtttg ttctttttat ttgcattatt
      421 ggcgtttttg ctcttgtacc ggtatttggc cagtcttcac cgaatcttcg cactattgca
      481 ggccttcagc ctggatcggt gttatttcta tcgattaagg gcgatgtgag tggagcctta
      541 tcgctttggg gaaccgacat ctataccctc gattccaatg ttgccgctgc tgcggtgcat
      601 gcaggggtta tagcaaacgg tcaaacaggg attgtggcag tcgttatttg tgatgggctt
      661 tcttcatatt ctggctccct cagaaatggc gtacagagca gctcatgggg aagctatggc
      721 ctgtccttca ggttcgttgg gcttgctcaa gcctctggaa gcctcgcgca agcgactccc
      781 agcgcgccac cagcaacaca aacaccgcct actcagctca agccacccac gccatccact
      841 ccaccattgc cacagccccc agtagcgagt cgcaatctct acgcctttga tgatccctat
      901 gtgctcaaaa gaaatgggaa ctttaaacca ggtactactt tctatgtaca agtaactggg
      961 aaagtctcgg gagcggatgt ctggggaacc gatatctata ccctagattc cgatctctgc
     1021 gctgcggctg ttcatgctgg tattctttcg gacgggcaga ctggcattgt taaagttacg
     1081 atactccctg gccaacagag ctacgtcggt agtacgcgcc ataatatcac atcgatgaat
     1141 tatggaaact atggaatgag ttattcgctc gagcctgtga cgcaatccta cgaccaagtt
     1201 gtgtggatga ttctagaccc cggtacggca gactatatcc ccaatgtgac agtgggtaag
     1261 agctatgttg tttgggttac cggcatggca gaccagcgca aggtttgggg caccgacatc
     1321 tacacttcag attcttatat tgcgaacgca gcagtgcatg caggaatcct taaaccagga
     1381 gagagcgcac ccgtcattgt ccatgtcctt gaaggacagc ctaactatga aggctcaaca
     1441 cgaaacggca tcaccacaaa ggcttatggc tcgtatgagc gttcctacac gctcgaacct
     1501 gcgcgatgag atgagaagca atgtgaatgc agagctcttg ggactatcta cctaagcgtc
     1561 aaatagacac cgttgcttaa aattagtaat gagtcggtgc cagtcagtat ggcaagaatg
     1621 caagcttcta ctcaagactc tttgcctttg gtagctcgtt gaataggtgg cacaggtaat
     1681 gatagggctc atggccatca aaaaatcctt gtgtcacgca catgaagtgc ttcgattgca
     1741 cagttgaatt tctcttatgc agcgcctgca ccactgtctc atccatacaa gaaggagcta
     1801 gcctctcgtt cagcctgagt gaagggggaa attttaatcg aactatatat ggggacattt
     1861 taatcgagtc ttgacaatct agttttttat gctactatta ttaaaccggt taagcaaccc
     1921 aacttaatcg aatagcatga aggaggatgt agtatgaaaa ggtttttgtt ggcattcatg
     1981 gcgctgttga cagttactgc tgcatttggc gctggtaaac ttaccttgat gcagaataaa
     2041 cccgagatcg atgctcagct taaagcctat gcagccgcat ggggccagaa aaatggcgca
     2101 actgttgtga tcaaatctat tggtggaacc tcgggcggca tgggtcctca actcaaagct
     2161 gactatgcgg caggagatat gccggatatc ttcgccttcg atggccttga ggcttataag
     2221 gagtgggaag gaattatact agacctctca aatgaagcct gggtttccaa gacctctgtt
     2281 cccttcaaat ataatggaaa ggtctacgga tttcctgtgg caatcgaagg atggggattg
     2341 gcctacaatg ctgacctttt ggctaaggct ggcatagatc ccaagacttt gaacaacttc
     2401 gacgcatata agaaggcctt cgaaaagcta gattctatga agaaggatct gggtatcaat
     2461 tcggttgtat ctatggccgc ttccattgag atgggatggg ttaccgctca tcataatttc
     2521 aactccttgc tatccaatgg cttgccttat ggcgacctat ctgttacgaa cgatctgttg
     2581 gctggcaagg tcgatgcaaa gaggcttcag gagtatgcag actgggtaga gcttctattc
     2641 aaatattcag ataaagcaat tcttcttacc ggcaactatg acgctcaagt tggagccttt
     2701 gccaccggca aggctgtctt cttgcaccag gggaactggg tagatcctaa cctaaaggat
     2761 gcaaatgcca cattcaagat ggcttttgct cctcatggat cgatgaagaa agttaccgat
     2821 ggtatcttcg tatcagctcc atcgtattat gcaatcaaca aggaatctaa gaacatagcg
     2881 cttgctaaga aatttcttaa cgatcttgtc gtaactccgg aaggaaataa ctacatggtc
     2941 aaggaagcag gcatgattcc agccttctcg aatattactc tcgggccaga aggtcagcta
     3001 tccaaatctg ttcagcagtg gtcggcagcc ggcaaggtct atagctggaa tcaatattat
     3061 ttctcgggcg attttagaga caaggtgctt actccgatct ataaccagtt cgcagccggc
     3121 agcatcagca aggctcaatt catcgatctg atgaccaaag cattcaagga taatgccaag
     3181 aagtaatcta tcatagtaga atacttttcc agcgaagctg tccgcgcaat tgcttgattt
     3241 ggtccgcctg cattgctaat gtggatgcag gcggttttgt ttttcaatcc tcgataaaga
     3301 aggggagcat tcatgacaaa aaaaatggaa tctgcattgg tattttgggt atttcttttg
     3361 cctgtgctag ttgcgtttat catggtaatg gtgattccat ttatattggg ctcctattat
     3421 gcgtttacta attggacttc atcggcaagg gtagatagcg tcttgaggtt tgtaggtttc
     3481 gagaatttta tcaatagcct aaaagatcca tcatttttat actctttcgg aattaccttt
     3541 ttatatacga ttttaaacat gatcgtcata aatattgtgg cctttgtgct ggctctgttg
     3601 gtaacgggcg atcttaagtt caagaatgta tatcgagtcg gctttttcct gcccaatctc
     3661 atcggaggga tcattctggg atatatttgg cagtttatct ttaataatgc tcttccttcg
     3721 cttggttcta tcatatctgc ctttgatttc ttatctgatc cagccaatct aatgctaagc
     3781 aagcccaaca ctgctatcgc cgcaatgatc atagtgggga catggcaata cgctggttac
     3841 ataatgatga tctatgttgc agcgatagaa aatattcccc aggaactcat cgaggcttca
     3901 agaatggatg gggctactcc atggatccgt ctcaaatcta taacaattcc tctgtgtgct
     3961 caggctttta cagtcacaat gtttcttacg cttgtcaatt cattcaaaca gttcgatgtc
     4021 aatgtttcgc tgacgagcgg tggaccttct actatgttga tgggtcaacc tatttttgga
     4081 acggagcttc ttgctctcaa catatacaat acagctttta tctcgaacaa gctgtccgaa
     4141 gcacaggcaa gggcgtttat ctttttcttg atactagcga tcatttcgat cattcaggtg
     4201 aacgtgaaca agaaaaagga gatcgagtta tgaaaagcag taagattaga agaccatcat
     4261 ctttccttgc cgaaatactc accatcattc tttttgtgct tttcttattc ccctttttcc
     4321 tcgtactcat caattcggct aagacatctt tcgaagtgac acagtatcca ctggcctggc
     4381 ctaaaaaatg ggagacgatt gtcgataata tcatcaaaat ttggaccagt gagtcggttt
     4441 gttatcctag ctccctcctt tcaagtgcca taattacggc cgtttctctg attttgatca
     4501 atgtcttttc tgcgatggct ggatgggctc tagtgaggac aaaaactaag gtctcttcat
     4561 tcatattctt catttttgtg gcttcgatgg tgattccttt ccagattgtc atgtttcctc
     4621 ttttatcatg gtttcgcacc gttaccgttg ctacaggcat aaggttgctg cgcacttatc
     4681 aggggattat tctaagttat gtcggctttg gtgcacctct ttctatattt atgtttcatg
     4741 gtttcatcaa aagtattcct ttggagttag aggaggcggc cactatcgat ggatgtcatc
     4801 ggcaccaaat cttttttagg atcattttcc caattctcaa gccaattcag gctaccgtgc
     4861 tggttcttaa tggtatatgg atttggaatg attatcttct gcctcttctt gtgcttggca
     4921 aaggcaatga tgtaatgact gtgcctttag ctgtatccaa ttttgctgga gcctttgtca
     4981 aacaatggga tctcattctt acagctatac ttatggcaat gattcccgta atcgtattct
     5041 tcctttttgc tcaaaaatac atagtcaaag gaatggttgc aggtgcaata aaataataat
     5101 tttttgatga atttagctaa ttctaaacaa tatcgaaagt tgcggtaagc tcaggaatcg
     5161 aaacccgtcg aagcgcaata gttatgttcg tatctggttt tacattacga gatatatgaa
     5221 taagcatttc gattccgaaa gatggtatat agatatgagc atggcctctg tcgacctcta
     5281 ggacaatcgc ttgacctttc caatctctat gtttgctgag gtaaaccatt gcccaatgaa
     5341 ggcgggaatc tctttcagct tggcgattct ttgccgcttg cagaccatag aggtagagcc
     5401 tttggtcaag catttctcgg ctgagtccta tttgcgcttc tctaggaatt ccttcctcta
     5461 atatattgct ggatttctgt cgggcaagct ctgcgtgtat ctgataatgc gctaacagat
     5521 cttgatatct tcgaagtggg ctagtcactt gagagtaaaa tgatagacca aggcctgcat
     5581 gagcgccgca ggttgagctt acaatagccg tccgcataac cttgcgtcgc gcatagtttt
     5641 cagcaaggct ttccgaaagt cccgctccgc atccagcaga ttcagcggag atgggaggct
     5701 cctgcgaaac atacggaaaa gcaaggccag ccgcataagc ccatcgtgct gctgcctccc
     5761 ctgccaaaat catgagttct tgtacaagcc ttgccgatct agttgcaggt acaggcaaaa
     5821 actcgacatt gccgtctatg gctctaatcg acacttcagg gaatacaata tccacggagc
     5881 cctgatcttg cctgagcttt tttcttgctt ccacaatttc atttaagttt tgtagtactt
     5941 cattagtttc tagcaagaca tcagcctctt cgtaggtaag ccttgctacc tttataatac
     6001 ttggtacgat agatatttga tcaattttgc catttttctg caaggtgatt ccgaatgaga
     6061 gagctttgga ttctggtaaa atgcccagtc caaaggcctc gatcaaggag tggtgaagca
     6121 tgggtattgt tttttccggt aaatataaag tggaagctct tgcgcatgca tcctcgtcct
     6181 cggtggaaat aggagcgatc gcggaaacag gatccgaaac atgtatccag actctattgc
     6241 cttctataga gatggcatcg tcgggatctt tggaccatgg attatcgatg gcaaaggaag
     6301 aaagatatct caagtccaat cggtccaagg tcaattcaga tcgttctcgg atattctttg
     6361 gttcggaagg cattgataat atgcatccat ttcgatctgg ccaaggatta ataaagggtt
     6421 cccaaaatcc ggttgcaagc aatgcttcat ggaccgcttg tggaatcaat ctgattccaa
     6481 gatcgcgcgc tattgggcta tcaactgcca agccgcgtgc aaacctactc aattcatcga
     6541 caaaaggctt gaaattctgg tctttatcaa caaaggatgg atcgttcttt ttccacgcaa
     6601 gcccgaacgc ctcaacgaaa gctgctctgg tttcggtgat ttcctttttt ttcctttcct
     6661 tttcaattaa tttctgttgt tcttctttgc ttcgaattct aaagccatta tcgagcattt
     6721 ctatggcagg gttttccagc agcatatgcc aagctataac gatatgcttg gcctcgactt
     6781 tgctccatgc aagttcggca aactcattcc aggagactat tggaacacct tccgggtttt
     6841 cttctttaag cagatccagg gcttcattta cttctttgct atgttgagca attggaaagg
     6901 agtgaatcgg gccagcatgg accatggcca ggtctttatt gcgtaccttg cgctcatctc
     6961 catctattgt cttgatcaaa atcctatcta aatcgtttga aagaacaatg gcaggtcttg
     7021 ttttatacat caccaaagaa tgaggggata tcatgtgttg tttataatct cgataatgtg
     7081 gatatacaag tgtgtatcaa tgatctattg cgaatgctga gcgcttctgt tatatttgag
     7141 aacaattggt gacatgccca tcctctaaac atgaatgtgg gagaatttac ggaattaagg
     7201 agaatagaaa tgacagcatt gttagttgga attggactaa tagtattagc tgtgctcatg
     7261 gtaattccag gaattttggg ctggtggagc gatgttcttg cttttcttcg gggtgctctg
     7321 cctgtaatag ctttcctgat cggcgccctc gcggttttta ttgggatcgc ggatattaag
     7381 gataaaatag aagagaaaaa agaagaagaa aaagaaaaag cagaagagaa gaacaagtct
     7441 gagaattccg aaaactaaga gaaggcaacc gagaacaaag gagactggta gctgcttttg
     7501 gtcttggatg cttgacagat ttttgaaact gtgggaatat gcacccgcat tttttgatat
     7561 ttctcaataa ggtgaaggcg tatataaatg aagaccgcat tttttaccgc tgcaaccgcc
     7621 gagcgcaagt ggtatgtaat cgatgcagcg ggcaagccac ttggccgcgt agcagctaaa
     7681 gttgcttcga ttttgagagg caaaaacaag ccgacattta ctccaagtca agagacaggc
     7741 gattatgtca ttattatcaa tgctgacaaa gtcgccataa caggacgcaa acgccagaat
     7801 aaaacatact atcaccatac cgggtatcct ggtggtatta aggattttag ttttgaccaa
     7861 cttattgccc gcaatccggt atctccaatg gagattgcaa tcaaagggat gttgccaaag
     7921 gggcctcttg gccgaaagct gttcaagaat gcaaaggtct atgccggtcc cgatcatccg
     7981 cacctagcac agaatcctat cgcaatagat atttgagcgg aaaaggaaag aaaatggtaa
     8041 agaatatcaa tcttggtatt ggtaccggtc gcagaaaatg cgcagtcgca agggtctttc
     8101 tgcgagaagg ttcaggcaaa atattcatta acgagaagga tctaaaggac tattttgcgg
     8161 ttgatggtct gattgcccag gtcagaaaac cgttccttgt aaccgatagc gaaaataaat
     8221 tcgacgcata tattacggta gtaggtggtg gtctatccgg acaggcaggc gcatgttctc
     8281 atgggcttgc ccgcgcctta gctcagatcg atccggcaaa ccacatttcg ctcaaggcca
     8341 atggcctatt gactcgtgat ccgcggatgg tggaaagaaa aaaatacggc cagcgcggcg
     8401 cccgccgaag attccagttt tctaaacgct gacgacctat ggcgaagcgg atcgattccg
     8461 ttcgcaaaaa ggcctccgga tcgctcgaag tggcggtttc cgggggcttt gtgttttgtt
     8521 ttgatgaagt agatgcacag cttctggatt attcattgaa gacccatccg tcggatttgc
     8581 ctttttcggg caaatatttc ttagatgttc attctccggc agaacactca gtggatgcat
     8641 tggcaagaca ttcatcagca gtgcacttaa ttgccgcgga ttcaatcgaa ttctcagaat
     8701 ccgcaattgc tggacattat ggaccaaaat ccattttgct attccaagat gaatcaatcg
     8761 atgaagactc gttattgctt ctcggtcgtc ttaatgatct gcatagggct cgctctgatg
     8821 ctcttttaat tgtttctcat aacgaacagg cttcaagaca attatacctg aagcttctaa
     8881 aaagaggata tgagccggag attgccaata cctgtgttcg atggacatgc gcggagcatt
     8941 atgttgatga ccgccgctat gtgagaatgc tctttaaatt gcactgtgtt aagaaagggc
     9001 aggggcccac caggatgcag gcgcttgcat ggcctcgaat cgggcttttc gaaagccctc
     9061 agacaatctt ggctgacgga atggctgcca ttcctgaaga cgatctcatg gaagccataa
     9121 agaagaatat tgagtcctat caaaggaagc cgtcaacatt ttcgcgccta gatctaaatt
     9181 ccaattcttc tgaaaagcga acttttttgc gttcattttt aagaagacag ggttttccat
     9241 atcagtcaat cgagaggttt cttgaaacct tggtagagaa ggaacacaaa aatgattaaa
     9301 aatactattt tccaatactt gacagacctt acaaatagat tcaataatat gtcagagaat
     9361 tcaagtcgga ggcgcatatc gaatggatga cgacatcctt actattgaag aagtagcgaa
     9421 gtacttgcgg gtttccgaac gaacagttta cgactgggct cagaaaggcg aaataccgtc
     9481 cggaaaaatt ggcactgtct ggcgttttaa aaaaagtgaa atagagcggt gggtgaacga
     9541 gcgcctttct tcgaacaggc ctgtttcgct catcaacagc gtaaaaatcc agaatatcat
     9601 ttcccttgat cggatcattt ttctggatta tccctccaag cgagatgcac ttatcaaatt
     9661 agcagaaaat ctatcgactg caccccaaat aaagaataaa gaagaattga tctcggaaat
     9721 tatccgacgc gaagaactca tgagtacagc gatcggaaaa ggcatcgcca ttccccacgt
     9781 gcggctgaat tcggttacgg accttgtggt ttctataggc atttctcgtt gcgatatagt
     9841 cgattttcag acgctcgatg atgtgccagt gcggctattg tttatgattg ccgccgcatt
     9901 taaccagcat gcatattatt tgcaaacgct gtcgtttttc tctgcaagac tcaaggtagt
     9961 tgagctgcga gatgggcttc taaactgcaa gaccacgcaa gaagcatatg atttgcttat
    10021 tcagcaagat cgatagcgct ttgctgcttc tgtgagagca cctgcagtgc gcctatgcgt
    10081 ttctgtgggt gctttttatt cggctacgaa gggatcgttc tgtgtactta aaaagccttg
    10141 aaatatttgg attcaaatcc tttgcggacc gaatacgcat cgagttttca aaaggaatct
    10201 cggctctggt aggtcctaat ggttgcggca aatccaatat tgtagactcg ataaaatggg
    10261 tggtaggcga acagtctgcg aagagcctcc gagcggagac gatggaagat gttatattca
    10321 acggcaccga aacaagaagg cctctttctg tcgcagaggt agcgattaca ataagcaatg
    10381 atgaaggtgt gctacctcta gatgtgcccg aaatagaggt aaaacgaagg atatatcgct
    10441 caggggaaaa tgaatacttc ataaacagca agccagcaag actgaaggag attagggagc
    10501 ttttctggga tactggcatt ggaaagtcag cctattctgt gatggagcaa gggcgcatag
    10561 atcaggtcct atcctcgaaa cctgaagacc ggcgatatct gtttgaagag gctgcgggca
    10621 taacgaagca cagagtccgc gcccgcgaag ctgaactcaa gcttgccaaa acagaggagc
    10681 atcttcgcca aatcgaagcc ataatcggag aagtcaaaca atcttacgaa aacctcagag
    10741 tccaatccga caagacaatc aagtataggc aacttaagga taagatattc gagaccgaat
    10801 tagatctcta tttggtccgt ttgcgccaat atatacgcga gcaggaccga gtctgcaacc
    10861 tttttgatca aaagaagaaa gaacgcgaag cacttgtcca gcgcattgag tctatgagtc
    10921 aatcgatgtc cgatgctcta gatatgttga acaagctcga agcaaggctg atcgagatgc
    10981 agaaactgat ctatggtctt gctgtcgaaa aaaatggcaa agagaggcaa aaagccctct
    11041 tgacagagag gcttagagaa atcaagtcaa taattgaaca actcgaggcc agagatcgag
    11101 ccatagcctc taaaatagaa tcgctccagg aagaagaggg cgataaagaa gcagaacttg
    11161 ctggctatag aattagaatt cgagaaattg aagctaatat tcatactttc gatgagaata
    11221 tctctgcggc cgctctattg ctaaaggcaa atgaggaaag cataaggcac aatgtcgagg
    11281 aatccaagga aattgcccaa aagacaaggc ttctacatga gaaactggat actatcaccg
    11341 agagaattgc agagcttgtg gatgagcgac ttcgcaaatc cgagtcgcag atagagcaga
    11401 aacagaacct cgaggcgcaa atacggcaaa agataaaagg aatatcgatc tccacatctt
    11461 cttgggcaaa caagttacag gattatacca gattagcccc ttctttgact gcccaagagc
    11521 gttttcgcga gctcgaaggc ctggtcattg aaatgcgagc cttgtcttct ctttcctccg
    11581 atctcgaaaa ggattttctt cattatgtgg aaatagaacc tggattctta aatgagctta
    11641 tagctcccga aggcattatg actcaaaagc gccgcatcga tgcggaaatt ttgaatcaaa
    11701 gagagcgcat tggaagaatc gaagccgaga atcaagagct gacgctaaaa aatacagacc
    11761 tggcaaaaaa aatcgagacc tatcgcaaga cactggaaga ggccagactg gagaagacga
    11821 agattcaagc acagatggac gctgcaaaag aagctctttc ggtactaaag agagaaattg
    11881 caggccagga agcttaccat cgcgaagttg ccggggagct cttagatcag catagacgac
    11941 acgaagagct caaagagcaa atagaattgt tccaggaaga gcttgcggag atggagcaaa
    12001 agggcagaca tctaacagag gagatgtcgg agctcgacaa atccattagt cgagcgactg
    12061 aagatcttgg agacaagcgc aaggagtcaa gcgaactcga gcagaatttg catgccattc
    12121 aacgggagct cgaagacctg catacagcaa aagcgcagag ggaaacagag attcgcaacc
    12181 tcaaggagaa ttttgtagat ctctattcga gagatttgct cgcattcgaa agccgcatgt
    12241 atgaaattcg ctctcctgtt tccgaaataa aggaaaaact tgtatccttg aaggcttctc
    12301 tgtcgtcgct aggttctgtg aatcttatgg ctccagagga attcgagcca gtacgacagc
    12361 gatttgaatt tctttctgga caatatgacg acctcgtaaa agcgagggca gaccttgtaa
    12421 ggataaccaa tgagataaga acagaaagcg caaagctctt tgtggaaacc tataataaaa
    12481 taaagaagaa ttttcacaat atatttcgaa ggctgtttgg cggaggaaga gcagagttgc
    12541 gcctttcaga tacggaccat gtcctggaaa gcggcataga aatctttgct caacctccgg
    12601 ggaagagact cgaaaacatc tccctgcttt ccggaggaga gaggacatta acagccatag
    12661 cgcttctttt tgctacatat atggtcaagc cctcgccctt ctgttttttg gacgagatag
    12721 atgccgccct cgatgaagcc aatattgtac agcttgttaa ccttctgaga gaattcggca
    12781 caatgagtca gttcattgtg ataacccata ataagaagac agtggccagc gccgacacct
    12841 tgctcggtat aacgatggaa gaatccggag tcagcaaggc aatcgcaatc cgtatccaaa
    12901 atgagaatgg tgttgtgcat ccagtctatg ttctcgacca ggctttcgaa gaagaggaag
    12961 tagattatga agaaggtagg cagctagagg taaatagatg aataccgaaa tgctggattc
    13021 aagattgttc tatctcgatc catggctgaa ggaagcggat gttgttgtaa agactataga
    13081 acccgtaacc gataggaggt cttttgtttt tggcgttatg ctaagcggga caatattcta
    13141 cccggagggc ggagggcagc cagcggattt cggttttatc gaagataaga gagttatcga
    13201 tgttcaggaa atagatggag agatttggca ttatattgag ctgcaagatg agaatgatag
    13261 agacagtcta aagcctggtt cgaccgttca tctgagactc gattggccaa ggcgtcttta
    13321 ccacatgcaa cagcacacag gacagcattt gctctctgcg gtgctcgaag aggaatatgg
    13381 cattcatacg ctttcttttc atcttggaac ggagtattgc acgatcgatg tttcggcaaa
    13441 agatccgatt gagcttcccc ttatgcagat cgaaacccag gtagaggcct ggatagggcg
    13501 agatgcggca gttctggtac attattgtcc tccggaagac atttcaactt ttaagctcag
    13561 gaagaatcct ccagcagacg aagccgttat tcgtatcgtc gagattcaag gttatgattg
    13621 ctctccatgc ggaggaacac atcttgatcg gacagggcaa gttcgcgctc tcaaagtgct
    13681 ctcgttagag cgctataagg gccatgtacg catgtatttt gcatcgggcg cacatgccat
    13741 agagctcatg tccaaggctt atgatgacaa tagagctgct gccactatgc ttggatcaag
    13801 cgttggcgat atttcgactc gactgagtga agtcttggaa aaatcttcct caactgagaa
    13861 aaaggcaaaa agaatcttag tacaatatgc cgaagccgag gccaggcttg catcgatcca
    13921 tgcaggaccc acaggtatac ttcagtttac gctggaaaaa gaagatgtag attggggtac
    13981 tatgctttgc aaagcggcag tatcgcttgg aagaccagca atggcttctt gccttaatga
    14041 ttccactatt atcatacagg tccccgcaaa ggctcgaatc cctgggctct cgaagatttt
    14101 ttccgaaatt atggcgaatt tgggtggaaa gggaggaggg ggagaggctt tttatcgggc
    14161 aagctttgct tcagcagata tagcaaaaca attcgccata aaggcaagag aagtactgga
    14221 aagatatcag actcaatgtt agggctagtc tttattgctt tttgaagcct taagccatca
    14281 acctctcggg cacctaccta tgccttcaga gcgctaaagg ccttctattg tcggccagtc
    14341 tcttactgag ataatcccta tcgagatcta gcttctggat aaaaaagcgc atctgctctt
    14401 cggataatag gttttgctcg gcatagagat agataagggc ccgttcggcg attagaggta
    14461 ccttaagaga attcatcagc atttcacgtt ctatcccttc agagcatcga ccaagaatag
    14521 atgaatagag tctcgattct tcgatttttc cctgaaaagc agccaactcg gcgaaaaggg
    14581 gcgagatgtt tccgcgggcg gcaagttcgc caaaaggctt gagagtgaaa aaagtatact
    14641 cttttgcagg caaataatga tgatcgtcgc atctagtgca ataatctggt tcacgaccat
    14701 cgtgcttggt tcggtctcct cctattatga tgagaggatc gaagtatagg gcagggcact
    14761 cctgattatc caccagataa tatcgatagg tgtagagaga atccctcagg cattcttcgt
    14821 gttcgcagta agctgtaagt ggcaccacat ctgtcgcggt atccagcaat agtcgagcgg
    14881 tttgattgaa aatctcgcga cgaaaattta gtaacagggt agggaagata aaacaaaggc
    14941 ctctcgattc agcttcatcc ttagtgagat atgctaaacg ttcgtcgaag aatgctgctt
    15001 cgtcgatgat ccaggtgccc acttcagggt tctctgctat aagcacttcg agcccaaagg
    15061 agtcttttat ggcgccaatc ctatttccac attgttcgta accaccccta tatgccaatg
    15121 catcctctgg ataatcgggg aaacgactct tatctaaggc agagcgaacc acaaatacat
    15181 ttcttcgatc gacattggct gtacttgtta gtctttttac cacttccttt ttttttagcg
    15241 ccacacggct gtctcgccag atgcgcgcgg caaattcggt tttgcctgag cccattgggc
    15301 caataacaag aattcgcttg ctcgatgaaa tgaaatcgtg gtgccgagcc gttgcatgga
    15361 tgtttagccg cggaaaacca aggctcttca gaaattcgcc tgtttcatct atgttggtca
    15421 tagtttttcc tggtgtttca attcatctca aggaaatgta tgagctagat gcctttgcta
    15481 cattcgggct tatgctggga gtaccaaagc gcaggtattt ataagggtgt atatcgaggg
    15541 cgttttgata ccatccctgg ataaagtcga tatcgatgac agatgccgca aagttatgat
    15601 atatcggcaa acaagcagcg ccttggagca attccgtttc ggctttggca agaatattca
    15661 tgcgatcgtt atcgctagca tcgtaggact ggatcagaaa gtccatgtaa gcagaatcat
    15721 tataacctgc tatatttaga ggagcatcgc tagcccacat ctgaaggaaa gcttccggat
    15781 ctgcgaagtc gccgatccac gtttcatgcg ctaccgtaaa ggaccaagca tccgacctat
    15841 taccgatgct atcataatag gacgaagctt gcattggctg cagtgtgact tccacatcga
    15901 gcgctttttc ccattcggtc ttaagagtat tggcaataag attcggtctt tcataatctg
    15961 caaatgctat gattatttcg ggaaggcctt gaccctttgg gtatccggct tcttcgagaa
    16021 gcttcatggc ttctgtgcta tcggattttt ctatgccttt gaccttcgag taaccaggca
    16081 gaggaagcac cagagtcttg gcaggcagca tatagaattc gcttgagcgg atactatccc
    16141 aaggtagaag aagagcaaga gctcgcctta ccctcttgtc attccagggc tctgaacggc
    16201 agttgaaaaa ccagtaatga gtcgaatata tagggaaaac ctgaatcgct gtttgaagga
    16261 gcactttttc ataatttccg ggtccgtcga gccaatgaac ataccccgta ttgaaaagcg
    16321 atgtagcgag ttcatcatcg tctgtaaaca tcattcgaag cattggaata ctgacatttt
    16381 cggagtccca ataagcttcg ttcttttcaa ggactagctc tgtttctgta atgctgaccg
    16441 gcttataagg accattaaca ggatagggaa ttgcacttct ccaatcttct ttatctatca
    16501 tcgaaggatg gattacagag aaggaatgat ggcagagtag ccttgtaagg taggcgacag
    16561 ggcgaaccag tgcgatttcg agcacattag ccgaaactgc cttaattccg acatgctccg
    16621 gatttttatc caagcccatg cgatattgtt ttgcaccttc gattatatcg aagaaagttg
    16681 catattcagc atttatgtcg agcattctaa accacgatcg aacgaagtct tgagcggtaa
    16741 tgggtgagcc atcggaccag atagcatcct ctcgaatggt aaagcgatat agcttcttat
    16801 ccaccgaacg ctcccagctc tgggccatgg cacgcaatgg ttcgagagtc tgtggatcat
    16861 aggtaaagag cccctcatac atagcgctgt gaatctgagc ttcattcgag aagatagcct
    16921 tctggggatc cagctcaggt atttgagagc catatactgt cacaaattcc ctttcctgca
    16981 tctgggcagc aagaggggaa aaagctatgg acaataaaga tacaactaga aaaaaccttg
    17041 aattcataca cttgattatg cgatataata gttcggatgg caataaacat gggaatcgaa
    17101 aaagaattta ttccggagag gctcattgtg ggagtgcttt cttctgatgc agaagcggaa
    17161 atagccgctc tcgacgcaat gatagggttg cacggaccgc tttgcttcca atcagacaaa
    17221 gagccttttt tgtggacaag atattactgt ccggaaatgg gagaggatat cttgcgttct
    17281 tactgggctt tcggagagtt gatagatcca tccagactct cgataataaa gcgcgctaca
    17341 aattccatag agcagaggct tgcaaggaat ggttgtagat tggtcaatct cgatccaggc
    17401 atgcttggaa ccgctcgatt ctgcttggcc acaaccaaag accgatcgca ccgtattccg
    17461 ctttcggatg gaatctatgc ggagttgact ttgatctttg agcacggaga attcaaggcg
    17521 cttccatgga cctatcctga ttgggcatcg gtgccagtga gaaacatgat gacagcgctt
    17581 cgagccgggc ttcttggcga tttaaggcat ctgcattttc tttgaatatg ttccgatatg
    17641 ttccgagaaa aagcaattct ccaaaacata atctcacttc agcatagaat tgatgtcgtg
    17701 cactctcaac acaccgtgct ttttcatcca ttcggtgatt ccattgagta tttcttgcgg
    17761 ggccagggga tttgaaaaaa gtgcggttcc aatctgcacc gcttgagcac ctgcgagcaa
    17821 atactgaatc gcatcggcag cgcgagtgat gccgccaaca cctatcacgg gaatggatac
    17881 cgcatggctg acgcgatagg tcgcagctac tccaatggga cgaatcgcag gacccgagag
    17941 accgcctaag cccttggcaa tagctggctt catgcgatct gtatctatga ccatgccaac
    18001 aaccgtgttt atacaagata ttgcgtcagc cccaccttct tctgctgcgc gagctatttc
    18061 cgttatatcc gtaacattag ggctcagttt tgcaattagc ggtctggcag tgcgccttct
    18121 caacgagcca gtcagtcgtt ctactataat agggtcgatt ccaaaagtca tacctccctt
    18181 gagtacattg ggacaagaga tatttatttc ataggcatcg acacctgttt ttgacatggg
    18241 tccagatagt gtcagttctt cctcaatacg gtcgaccagc atggcgtatt catcttcggt
    18301 cgagcctgca atgttgacta tgatcgcaca gcagtgactc ctcaagagtg gcagtttttc
    18361 tctgagaaaa cactcgaggc caggattagc tagaccgata gaattgatta gtcccatcgg
    18421 tgtttcaaca agtctcggcg cgggattgcc ttctctcggt tcaagagtta cggtttttgt
    18481 atatagagca cccaattcat cgatctggac aagctcttta tattcttcgc catatccaag
    18541 tataccagaa gcgactccca cggggtttgt catagtaaga gaaccgattt ttacggataa
    18601 atctggctcg aaggaggcag agaaatcatt taggtctttt atcgagttta tcaaaggcaa
    18661 gcctcccaat cgatggcatc ggcttcgaaa acagggccat ctgcgcagac acgaagatag
    18721 cctccctttc gagcaggaag cacacaaccc tgacaagctc ctatgccgca agccatccat
    18781 tgttcagcag aaagatgggc tggaaagtta tttcgttccg caaaagccgc caattctgca
    18841 atcatcgccc caggaccgca accatatata tgccaaatct cgagcgattt attgtccttt
    18901 ttaagacatt ccagggcatc tagcactgtt ccatggaagc cttcagatcc atcgtccgtt
    18961 gccataaaag ccccggataa gagtttctcc caagtattta atattggaat atcgtatgtt
    19021 ggttctttga acttaggaac aaaaagcgta ttgcgaaatc cgaagacgag ttgtatttct
    19081 tttccgctta tggaagtttc gagtgatttc gaattatttt caatatagct ttgtgcaatg
    19141 gttccatttt ctgccagcga tgcggccaga aatagcattg gcccgattcc tattccgcca
    19201 cccgcaatta ttgccttttc atctccatcg ggataaggaa agctatttcc tagcgatgca
    19261 atgatatcga tacggctgcc aggctgttga agggcaagag ctctagtaca aggccctctg
    19321 atctgataaa tcgagtgagc gatagcgctc tgatagccgg atgattttgc ggcatgtttt
    19381 ttgggttcga aacctgcaaa ggccaaaggc cttctcagaa tgattcccgc actcgattcc
    19441 accattgcag gcaaaaaagt aaagaactgg cctggtaatg gcggcagtag atcttctgac
    19501 cattcaaagg ccaaatgcat caaatttgga gcgatggatc tattcaggat taccgccgat
    19561 ttgaattgct tcaaagtctt cacctgagcg gaattctaac agaagaattc tggatttgat
    19621 accatgttcc aaataatgca ggagttcaac tcagtgatgt agatatttct caatttcggg
    19681 cttgttgaag ccaactatga ttttgccgtc tatatcgata accggtacgc ccatctggcc
    19741 cgattttctg accatctcct ccgcgcgtcg catgtcggca gcgacattgt attcggtaaa
    19801 agagatgccc ttttgctgta gccattcttt ggccattttg caatagccgc agcttggcgt
    19861 cgtataaata gtaacagaca tatatttttc tccctattgc atatggcagt tttgattcat
    19921 agcaatagat atagtaatct catttcatag taaaaataat atatattgac atttgtgtca
    19981 attgtcccaa acgaaaaaaa tgccagaatg tttttaaagc atactcgtct tcaactagcg
    20041 tctttatcat atgaaaagag taagagcagt tttagctttg attctgcatt ttcttagctg
    20101 ctcgatgcta gcctggacag ccgagacatc tgagaatcct ctgtctggca atctctcatt
    20161 tagtgcttca tccaaaaaaa ccaaactaac tctattctta agacatggtt ctgatgctct
    20221 tctgaattta tcgattcctc tatcgatcga taagaattgg ctcgagaata tagcatttac
    20281 gggtaaggca aggcttgcag ggtatgatat aaccgcagga aagcttgcgg taaaagattc
    20341 ggctagtttt ttgattgcac ctgtcctggc atctgctcaa ggaagatcag cgctatcgat
    20401 cagcgatagc ggaaaatcga gcgatccaat tggcatttcg atcgcagctt caagaatatc
    20461 atttttggca ggaaaaggag acccctatgc ttttgcttca atgcaatatc tatgtgcaca
    20521 agaaggcttg cttttctcaa tcgggtttgg aacgcttctc gatatcgaga gctcgataga
    20581 tgtactggat cgtcttgatc catgggcatc gatgggaatt ggctatacaa aaccttcagt
    20641 atctatcatg gtcagatctc atttatatcc agattttgaa aagagaagcg aaaatctgtt
    20701 ggatattccc tggttgaaga aaatcggcat gaggttagac atgaatatat ttgctggcag
    20761 tcaaaaattg cagggttttc tttatataga gtctggacat tttctatctg cgcttggaaa
    20821 ggtggcgagc tacgatgcta tggctcagat cggctatgac acagatatat cctttttgcg
    20881 aatcttgaag agccttaatg ttagcctttg cgcattttcc aaccaaggtc tggtcgagcc
    20941 tgtggatgca aaaaccccat cattcggcac tttgccagat ctatggacca tgcaatattg
    21001 gcctgacggt gggaaaatcg gattgaccat cgaaagtcag aaagcagcct tttcgttatt
    21061 tcggacggct ttgaacttga agaccagcat ttctggggct ttggcaataa ataccaaatc
    21121 ttggtccaat aatatagctt tagatcttat gcttttgaga gccgcttcct ctgaaatgtt
    21181 caaaatcaat ggaactcttc agggcagcca ttcgatcggg cgtaaattga tagaaatagg
    21241 agaagaagaa agctcggaat ctattggcaa tgaagaattg gggtcaatcg aagaaaccga
    21301 agaccaggct gcgagcccag atcatggttc aagcttaagc cctgcattcg acaaacttct
    21361 gctatccgga cgattaaaga taaacccatt ctcattctta atgtccgtga atctgcctct
    21421 ccacaatagg aattcaagag ctacatctgg gagaatcgga atgtccttag caaagcgtca
    21481 gttgcttatg gagctctcgg tatctatgca ggtcgagcca gaatcgagac agctgcagtt
    21541 taaatccttt agttgttatg ccaagattcc gctataagat tattttaatg caaagggtag
    21601 ttttcaattt tctctctgtg cgataccatt ttgtataata caagcctttt tacatctttt
    21661 aagggaggaa ttgagatgga tgatgctctg attgtcgtgg acattcagaa tgacttttgc
    21721 cccggtggag tccttgctgt tccggaagga gattccataa ttccaaaggt aaatgaactg
    21781 ctctctgcat attctcttag cattctaact caggattggc atccggctat gcactgttct
    21841 tttgcttcct caaaaggact tcctccattt tcactcgata tgggaacaag gcctccttcg
    21901 gtgctctggc cagatcattg cgtagcagga agccagggag ccgattttca tccagcgctt
    21961 caatcctgga gagctcgcta tatctttaga aaaggaacga gaaaagaact agatagctat
    22021 tcggccttcc tcgagaatga tggcgttaca tctacggggc tcgcggggct tttgtcttcg
    22081 cttggagtag ataaagttat tgtttgcgga ctggcaaccg attattgtgt aaaagcaacg
    22141 gctctggatg caagaagatt aggatttgag gttgtagtgg tagaagatgc tattagagga
    22201 attggagcgt ctccggacga tatagaaaac gcccaagtac agatgcgtga agtgggatgc
    22261 gtctttgcgc aatccggcga cctgatggca tcctatacta tatctagtat ttctccatcg
    22321 aaaggaatta tatgaattgt atttataaga acgccctctt tacggatttc tatgaattga
    22381 caatggccca aggctactgg aagcgtgaga tgacaatgcc tgtggtcttc gattatttct
    22441 ttcgtaaaca tccattcaat ggtggctatt ccgtatttgc tggtttggcc acccttatcg
    22501 aagccctcca ggaattttct ttttctcagg atgatctgga atacctctcg accttgggtc
    22561 tcttcgagga tgaatttctt cactttttat cttcttttag atttaagggc actgtatatg
    22621 cggctcgcga aggcgaaata atcttccccc aagagccact tgcccgtatt gaggctgacc
    22681 tcatctcggc gcagatcgtc gagggtctgg tgctcaattc gttgaatttc cagagcttaa
    22741 tcgctacaaa gacgacgaga atctggaatg cttcacaccg cggttcgatt atggaattcg
    22801 gtttgagaag agctcaggga gctgatggag cgctctctgc aagcagggca gcttttatcg
    22861 gtggtgcaat ggggacctca aacacacttg cgggaaaaga gtatggaatc tcggtgcttg
    22921 gaacaatggc gcattcctgg gtgatgtctt atccgagcga gctcgatgca ttcaatgctt
    22981 acgcagaaat ctatccacag aacacaacct ttcttataga cacctataat tctcttgaat
    23041 ctggcattat aaacgcgatc gaagttggga agaaactgaa agaaaagggt tatagcttcg
    23101 gagtgcgtct cgattcggga gacatcgatt acctctccag ggctattcga gctagactcg
    23161 atgaagctgg ctttgaagat gcaaaaatcg tcgtatcgaa tgagcttgac gaagagataa
    23221 tagaaagcct cgtagacgat ggcgctccga tcgatatctg gggtgtaggt acgaatcttg
    23281 tgacaggtgg aaatgaagct gcgttttccg gtgtctataa actcgccggc atagatccag
    23341 aggataggct gcggcctgtt atgaaattct cggataatcc agaaaaatcg accaatcccg
    23401 gtgtcaagaa tctttggcgc ttatacgacg aaaaaggcgc tgctcgactc gatcttattt
    23461 cttgtcagga tgagcaaatt caggaaggaa tcgagtatat tgtgcaccat cccagcgccg
    23521 actggcgccg tttgaaaatc actccggcta gagtggaacc tctgcttttc aaagtgatgg
    23581 aaaaagggaa tcgaacagca acgcttcccg atataaggca gatccaggca ttcatgaaag
    23641 agaggataca ggcctttgat tcgacatatt tgcgccttct caacccacac atttacaagg
    23701 tttctattac cgatagactt tatgatctga aggtatttct tatcaattcc tttctaaaac
    23761 ataagctatc gcgaaattga atttcctcaa ttgtgatttc tgcgctctgg tttttttaag
    23821 aatggcgctg cgctggatgt ggtggcttta tttcgaaggg cgtcgagtaa ataccctatg
    23881 tcatcgctaa ctatgagatc ttcatagatt tctttatgta gaaagccttc ttcgaccatg
    23941 tgcttcagca tttccaatag atgatcgtaa taatgctcta tatttagtaa ggctacaggc
    24001 ttttgatgat aaccgatata tcgccaggac cacacctcga aaagttcctc cattgttcct
    24061 ataccgcccg gcatgcatag aaaagcatcg gctttttttt ccatgagagc tttgcgctcg
    24121 tgcatatcct gtacgacgag cagttctgag acctcctgtt ggtctacgag ttcatacagc
    24181 cgtctaggaa tgataccaat agctttccct ccatttttca tgactgcttc agccacaatg
    24241 cccataagcc caacatctcc tccgccatag acgaggacca tcccttgctc cgccaatgtg
    24301 cgtccgagtt tttctgcggc ctcggcgtac gataggcgtt tccccatgga agagccgcag
    24361 aaaacacaga gactcaaaag ttttgaagat ctattggtat ttgcatccat tatgtcttta
    24421 tctccccacc atcatccata cagatttgta caaataaggg ctttctgaaa ataagtcaat
    24481 atattgaaac tcttataaag aacaggcaca ctgtgagata aggccttgaa tcgaacggat
    24541 acggaagaac atctttcatt acactggagg tggggaatcg aaccccacgt ctttatcata
    24601 gaatagcctt gaatatcagt atttcccctg aattggaacc tgcctgttct tcatatattc
    24661 tacacgccgc gcatggaatt tcaatgtatt ttggcgcgcc aaatattcca tggtaacgag
    24721 taagctctcc attgggaatt tgataatgct acatggaaga aatactccgc tttaaaacat
    24781 taaacaaatg agcccacctt aagaccactc tagc
//
LOCUS       DAOOWY010000019        24129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2102_length_24129_cov_9.956758, whole genome shotgun sequence.
ACCESSION   DAOOWY010000019 DAOOWY010000000
VERSION     DAOOWY010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 24129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24129
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2102_length_24129_cov_9.956758"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /gene="ptsP"
                     /locus_tag="PK105_02850"
     CDS             complement(<1..1381)
                     /gene="ptsP"
                     /locus_tag="PK105_02850"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOU60678.1"
                     /translation="MMIIKGIGVSPGVAIAPAFVHIDDSGIIPQYHIAIDDVPSEMAR
                     FHEASLLAKKEIEALRELAKNEAGEEQAAIFDAHIMMLDDPEFLEQIERSLKEKQFNV
                     EVAILSFESEMVNRLSSSLDPLKQEKVSDIHDVVRRLLGHLLKKERISLSDIQTEIIL
                     VAKDLLPSEMVMMSRSMVRGIVTETGSRTSHAAILARAFEIPAVLGVGPGFILQVRQG
                     QSVYIDGDNGTVVIEPDDSIIQSEKSARAARLNQEKENRELRDVPAHTKDGTSIMLLA
                     NIGMPEEVVNAIEHGAEGIGLFRSEFFFLGGHIPGEEEQYNAYSQVARSMHCRPVVIR
                     TLDIGGDKVLPELGVIGEKNPLLGWRAIRFCLEKTDLFKTQLRAILRASGQGKVNIMF
                     PMISNIDELIRAQAVLAEAKEECKSKGYKIDEDIETGIMIEVPSAAICSDVLSRSCDF
                     FSIGTNDLSQ"
     gene            complement(1470..2402)
                     /locus_tag="PK105_02855"
     CDS             complement(1470..2402)
                     /locus_tag="PK105_02855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU60679.1"
                     /translation="MRIKLDQSNWVYYIGRGSNLGKLVFSVGCMLMLLGSSFRPAPGP
                     MTARGGSDVSDFAKSNEIAMPEPEDKLVKYSVYEVKKGDTLSEIAEYFDLTLDTLISI
                     NNFKSAKSLKPGQLLKIPSESGILYIASKPTTIQEIADSYNINIDRLIETNGLLSETI
                     PAGKPLFLPDVKLPAATLREIAGTLFQWPVKGRLTSWFGWRKDPFTGRRSFHNAIDIA
                     APYGSSITAPMDGRVIETGYSPILGKYAIISHSGGWKTLYGHMSEILVQEGQYVSQGR
                     TIGRIGTTGYSTGPHVHFEVIKNGVLVNPLNYLP"
     gene            2541..3707
                     /locus_tag="PK105_02860"
     CDS             2541..3707
                     /locus_tag="PK105_02860"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU60680.1"
                     /translation="MKPERADVALIRCESYEAEKLDKTIETCAEIASLPDLQGKTILI
                     KPNVLNASPASKAVTTNPAFVEAVIRFAKSRGAKEILVGDSPGWQPVMLAAKTSGIYD
                     AVQQSGGTWADFREVSPHAVKEGKKLKNIPLTSLVEEVDLVINLPKLKNHGLMTYTGA
                     IKNLFGLIPGTAKSAMHLQYPAVIDFGEMLVDLALSIPNCFTFMDAIIAMDGQGPGSG
                     TPYSLGIVLASRSVAKLDWIASQCVGYDPRNISYILDAMERTSGSANITEPSTYPLRV
                     SEIEHSGFRRLPYTSKLGKRLSAIPRGLRSLAGKLIHLRPIFHKNKCIGCSACIEICP
                     AKVLLLDRKNGSNIIRINDKLCITCFCCHEVCPAKAITVGRAPVRIVHRRLPGL"
     gene            3723..5360
                     /locus_tag="PK105_02865"
     CDS             3723..5360
                     /locus_tag="PK105_02865"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOU60681.1"
                     /translation="MASKAGKGGKRVAHVSLDGLIRFGNLSDLETMDLIAPMEKNGYL
                     VLQADALRECSRIAFTKIAYLMPETQYDAFAKIIEDPEASEYDRFVASCLLENALISG
                     KRRYPICQDTGTALVYGWRGDRIEIEKNIDLEELLVQGAAQAWTQEKLRNSQMGPLNM
                     MEECNTKDNLPAGIQIRSIKGKELRLTFVAKGGGSASRSSISIESPAILRADKLENVL
                     RLRINALGPSGCPPYAIAMVLGGTNPAQSLYAAELAALGLLDNLPTSANGDGMPMRDI
                     LWEKKMLTIARDSGFGAQWGGSHFAVSTRAIRLSRHAANLPLAIAVSCAANRHVSVFI
                     DNSGWYIEKLSPPSQCLENKAQEIILQTSAKAEIIQITQSDDSWLSKLRSLQAGTMAL
                     ISGPVLLARDMAHARLLRMLESDKKLPDWFIGLPVFYAGPTEAKQGAKTGAFGPTSAS
                     RMDSYLEAFMKVGASMVTIAKGSRSNEARRAIATHRGVYCAAIGGVAALNAERFLQKL
                     DTIQFEDLGMEAVRIAVIDRLPVIIAIDSHGKDIYDS"
     gene            complement(5381..5959)
                     /locus_tag="PK105_02870"
     CDS             complement(5381..5959)
                     /locus_tag="PK105_02870"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU60682.1"
                     /translation="MVAGDGNGGRKWMEISTRKVYECNVFSVLERESKGPDGRTGHFS
                     LMRAQDWAVIVPYVRTDAGTSFLMVRQYRHGAEELSLEFPGGVVENGEEPSHAAAREL
                     AEETGWVSDKILHAGTVFPNPAIQDNHFHVFVALDPKPAVDRHLDQNEIIDAYLVDAD
                     EVRRRIGEGELRHALMVTALYLADRLVGSLKA"
     gene            5997..7007
                     /locus_tag="PK105_02875"
     CDS             5997..7007
                     /locus_tag="PK105_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOU60683.1"
                     /translation="MGKLSMKLCNFPLSKFREILIINKMNIKPCNRLRNKALRVFFLM
                     GAGLALFGQNIPKSLSMEKLIMQSYRPIVIAHRGFRSIAPENTLLAAQKGFDIGADMW
                     ELDVAASSDGILVVLHDNTLTRTTDAKTRFPSRSPWIIYDFTFDELKSLDAGSWYSKT
                     DPFMQIAYGRVGSSELESFKDLRIPTLREALELTKRLGWRVNIEIKDATGFACDAWIV
                     EKTAELIKELDMVPSVLISSFNHDYLKRMKMAIPEISVAALIDKPISDPVQRLKDIGA
                     VALNPNEKYLDEATVRALRSAGLGVLPWTVNDPSRMRMLLNWGATGLITDFPDRALEL
                     VK"
     gene            7130..8431
                     /gene="ugpB"
                     /locus_tag="PK105_02880"
     CDS             7130..8431
                     /gene="ugpB"
                     /locus_tag="PK105_02880"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HOU60684.1"
                     /translation="MKRVVLLIAVLLMAVGAYAQTTVELWHAMAGHNGEMVQIISDQF
                     NASQKQFKVVPVYKGSYVDTMNAGIAAFRAGQAPAIIQVYEVGTATMMAAKNAIKPVY
                     QLMAENKINWSSDIYIPAIKSYYSTSDGRMISMPFNSSTAVMYYNKDAFRKAGLDPEK
                     PPVTWPEFFEVAKKLKASGMEGGFTTNWISWIQLENFCAWHNIPFGTRSNGFDGLDTQ
                     LVFNSPIVVRHFQNIYDLSKTGVFIYGGRENKANPLFTSGRVGLHFESIGGYGNMKAT
                     CKFDFGVARLPYYPDVPGVPQNSCIGGASLWVFNGRPAAEYKGVAEFFNFISKPEIQA
                     LWHKETGYLPVTQAAYELTKSQGYYVNNPGLEVAIKQLTNKTPTPNSMGIRFGNMPII
                     REIEDQVWEEILAGKISVKDGLDKMVREGNKQLRSFEKLNK"
     gene            8606..9490
                     /locus_tag="PK105_02885"
     CDS             8606..9490
                     /locus_tag="PK105_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU60685.1"
                     /translation="MSKQYEFRASILPYLLVLPQMLIVFIFFFWPSAQAILQSFYLQD
                     PFGGKMIFVGFENYSNLLIDPDYWQSFWVSTVFALCITISSMAISLFLAIQANKKIRL
                     ASFYKTMLIWPYAVSTLVAGVIWLFMFNPNVGVIAYTLKHTFHIDWQYLLNFNQAFLL
                     LTMAASWKQLAYNFVFFLAGLQSVPATLVEAAEIDGAGPTKRFWKIIFPLLSPTTFYL
                     LVMDLVYGFFETFPIVHSITSGAPGKSTAILVYKVWRDGVINLDLGSSSAQSVILMIM
                     VIVLTVFQFRFIERRVTY"
     gene            9507..10382
                     /gene="ugpE"
                     /locus_tag="PK105_02890"
     CDS             9507..10382
                     /gene="ugpE"
                     /locus_tag="PK105_02890"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HOU60686.1"
                     /translation="MNEQQLSNKAAHRAKVLRSLYPHIILWIGIVIIIFPIYFIFSAS
                     SHTNADIQAAPMPLGIGPHLIENYRQALLHGTQNMGTPVLTMLKNSLIMALGISIGKI
                     VISLMAAFAIVFFDFPLRGFCFWSIFITLMLPVEVRIMPTYKVVADLGLLNSYAGLIL
                     PMIVSATAVFLFRQFFMSVPREIAEASQIDGATPMQFFSHILAPMTRTPIAAMFVIQF
                     IYGWNQYLWPLLITTKPEFYTLLIGINRMMSGADVQIEWQIVMATTMLAMLPPVIVVV
                     LMQKQFIAGMTETEK"
     gene            complement(10513..11952)
                     /gene="uxaC"
                     /locus_tag="PK105_02895"
     CDS             complement(10513..11952)
                     /gene="uxaC"
                     /locus_tag="PK105_02895"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOU60687.1"
                     /translation="MADFLDEDFLLKNETAKRLFFEIARDLPIFDYHCHLSPKAIAED
                     KPFDDLAKIWLEGDHYKWRIMRANGAPESLCAGAGAWDEKFLAYAGALTKAAGNPLVQ
                     WSHLELKRIFEIDEVLTPQSALSIRNKANRILSEKSISPRSLLEHFKVSVLCTTDDPV
                     DTLAWHRAIAEEQLSGSSFKTKILPAFRPDKAMKTEDIETWNVYIDSLGEAAGIEIDS
                     FASLADALASRHAYFHSMGSRLSDHALLVPPFVQAIDTELDCIVKKGRSRIALSQIER
                     AKLETALLLHFGRLNAKKGWTMQLHIGALRNINPGLNRVFGPDVGGDCTSDAPIIAPL
                     ASLLETLEREGNLPKTILYSLDPSKHNPLAVLAGSFAGTAPEISSPQVAGKVQLGAAW
                     WFNDQKDGMERHMKEYAGVGLLGAWVGMLTDSRSFMSFPRHEYFRRLLCNIIGEWVEA
                     GELPDNPLYTEQLVRGICWENAVNYFEML"
     gene            complement(12024..12851)
                     /locus_tag="PK105_02900"
     CDS             complement(12024..12851)
                     /locus_tag="PK105_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU60688.1"
                     /translation="MSFIESMFSLEGKIAVVTGGGGMLPSYIAVAFAKAKAKVVLWGR
                     GTHHPMEDAARMVRKQADLPIDCSDVIGITVDTGSTDACEQALEQTIHSFGLPDILLN
                     GVGGNIGRSKFVELDEKIFEEVLKLNLMAGLVIPSKVFAKRWIAAGIKGCMINMTSMT
                     SYKPLSDTWAYDAAKAGVLNLTQGLAKEFAPYGIRVNAIAPGFFIGNQNRALLIDEET
                     GDLTPRGQAIIARTPFGRFGEYEELWGTALYLASSKASGFVTGVSIPIDGGFLIDNI"
     gene            complement(12905..14206)
                     /locus_tag="PK105_02905"
     CDS             complement(12905..14206)
                     /locus_tag="PK105_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU60689.1"
                     /translation="MDVTIPTIILMGGFVLLLLLRVPITFSLAAASIATAIYLHIPLM
                     AIVQQMVQGVKSFSLLAIPFFIIAGEMMARGGIAEKIINLANVLVGRVRGGLGMVNVV
                     DSMLFGGISGSAVADVASTGVIIIPMMTKNGYDNDFSVAITVTSATQGIIIPPSHNMI
                     IYSVAAGGVSVGRLFLGGYIPGILLGISLAVIVYFIAVRRNYPKGRKYSFKEGIKIAI
                     DAFWGLLVAVIIIVGVVAGVFTATESAAVAAVYAFIITFFVYKQIPLREFRSILYSTL
                     KTLTMVMTLIATASSFSWLMAYLRIPAMATEILLSITDNKYLLLFLVNVLLLFLGMIM
                     DMAPLILIVTPILYPVLIGKFGMSPIHFGIMLMINLAIGLCTPPVGSALFVGCAIGKI
                     SIEKATKAMIPFYIAMIATLFLVTYVPEITMFLPNLIMPGQ"
     gene            complement(14211..14750)
                     /locus_tag="PK105_02910"
     CDS             complement(14211..14750)
                     /locus_tag="PK105_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOU60690.1"
                     /translation="MKKFIVSAVTLIHILLINLSMIMIVAMLLLVFVNVVLRYVFNSG
                     IYWSEEVALVLEVWFIFLSLGLGVKYRLHFSINLANREKISASLNRALDILTDIIYIF
                     IGAVMIIYGSKLVRFTMSSVMPATMLPAGILYLVLPLSGFVAIVEAVFHVFGFNMFDE
                     KLEAYFSGQGIRIRDVLRS"
     gene            complement(14877..15854)
                     /locus_tag="PK105_02915"
     CDS             complement(14877..15854)
                     /locus_tag="PK105_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOU60691.1"
                     /translation="MKKFGIIALALLLILLPSMLFAQAKPIVLRLAETHPKGYPTEMG
                     DEEFARLVKERSNGRIIVEVYPGSQLGEEKAVIEQVQFGAIDMTRVSISPVAAFVPKL
                     NAFQMPYLYRDSDHMWKVLKGNIGMELLASLEPFGFIGLGWFEAGARSFYNSKKPVYA
                     PSDLKGMKIRVQESELMLGLVRSFGAIPTPMPYGEVYSGLQTGVVDGAENNPPSYYSA
                     SHYEVAKYYTLDEHTMVPEIIIGSKISLGRLSKADQDLIKQAAMDAIDYQRAQWDAYV
                     KVSMDKVKAAGCTIIPIPDKTEWMKAVEPMYKQQSKEIQDLVARIRAVK"
     gene            complement(15993..17369)
                     /locus_tag="PK105_02920"
     CDS             complement(15993..17369)
                     /locus_tag="PK105_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU60692.1"
                     /translation="MIRILVADDEELERRAMIRILNSIPSNEIPDVMEASNGIEALDI
                     LQSQKVDIVFLDIHMPGVDGLKVAEKLSFLSNPPVIIMVTAYDYFSYARQALRYGVIE
                     YLLKPASTAEVHNALFLALREIKARKAEEERQAEAKRVTAGLKDFLQSKVCASLSENT
                     MDTEIIRQLVKLETGSETWLCIALVAGMKKFSSDDVLSLASCEFQRTFPVLAEKFLLS
                     DIGLAKANPMLFISPRGLRMEAMADEQMPDPIINVLIIIPHEIQKEEGNKIFQELFLY
                     RYSEHFEDQSIGRFLCRCRDAGAKTLRIGISAADYRSASIALRMATTAFNLSSVERPI
                     IVLNPIQTDEPKAKDTHKIASIALLWIEDHFMENIGLKDLAKAINISPSHLSRLLMKE
                     TGISFGEILSRIRIARAKSLLLNGISVKVTSFLVGFRDQSYFTKVFTKIEGISPSRYI
                     EQFQSRSN"
     gene            complement(17366..18982)
                     /locus_tag="PK105_02925"
     CDS             complement(17366..18982)
                     /locus_tag="PK105_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOU60693.1"
                     /translation="MHVISRRSSSADFPSKGGLGFHRPGIMGKILICFGIIIIFFLVL
                     ALVMQNESMNLSREYSKNLSNYLLVHRFRLNLNSFHAQADRYLREPASVEINSIYDGI
                     LLLNSQYVELLPIADISIYVEFEVRATGYGLDAYFPLVSRAVGLRAGNSPDYYQVFVN
                     ASRIHGYIDIYLNRMLSELMKNGEETYVSLSHKAEILNRTIFFSMIFASAIAIGVILF
                     VTQAITAPLRRLAKEAEKLAKGDLDAGIVEVHTGDEVETLTRSFATMAMSIREYVEGL
                     KEKSELERLLHEEELALVSMGKALREAQFLNLQEQMKPHFLFNALNTIARSALLENAP
                     RTESLAICLATLLRATIKDSGALSTLDEELVIAQAYLEFQQVRFGDRLQWNIDVPRSL
                     RKTRVPRLMIQPIVENSVRHALEPKVSGGFISIKAQKRKEGLVLWFLDNGIGMSKERL
                     ADVRAKIAASLQVQSFDQINLPQVAEIPFSEENDVNEEELLEGRSIGLANIATRFGIL
                     YGDSCRFEINSRKEKGTLVRITLPLGEKVG"
     gene            complement(18948..20183)
                     /locus_tag="PK105_02930"
     CDS             complement(18948..20183)
                     /locus_tag="PK105_02930"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOU60694.1"
                     /translation="MLQLSRFSIGTGDRFGREGEAQIGAFKDMRAKGGEADIVWNKSN
                     REHVLIGSTPADQEKAASESIKKTGWDGRWFVDADHITMKTVDWFLPYCNFFTIDVAE
                     SIGQNASLESRSCFLDRAAFLLEDSSVPVRVEHADLESVADKFLAAVEEAGKTYRHIA
                     EHKPEGSFVVELSMDETDKPQTAAQIAAILVAVDAEKIPISTLAPRFPGKFLKGVDYI
                     GDATEFFHAFEDEAKVLLWASEYLNLPKGLKLSVHSGSDKFKLYKGIHDITARLEVGV
                     HLKTAGTTWLEEIVGLAEAGGKGLSVAKSVYEQAYLRIEELTAPYANVVEIDAQMLPS
                     PKMVAGWDSDTFVAALRHEPNSSTMQPSMRQLMHVGFKIAAEMGREYLDALEECRESV
                     SRNVRYNLYARHLAPIIFG"
     gene            20212..21576
                     /locus_tag="PK105_02935"
     CDS             20212..21576
                     /locus_tag="PK105_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOU60695.1"
                     /translation="MSLERKERKLFLKDGTIIMNMMNIAEKRRESGIGRLELEQLVDR
                     TIKESGALDASKILIIPPDITRFHSRAGLITDLLVEQLGSRVAAVLPAIGTHTSMTEN
                     EIAQMYPLCPRTLFRVHDWRKDVVELGTIPEERVAEISGGKVRYYYPIQTNKILAFGD
                     IDLVFSIGQVVPHEVAGMANHAKNIFVGTGGKEAIDKSHYLGATCGMESIMGRIDTPV
                     RNLFDDALDLSKTRLPAIVWILTVIGTAGDGSLMQQGYFAGYDRKCFEEACALSAEIN
                     IALFDKPLRKAVVWLDPSEYRSTWLGNKSIYRLRMAMADDSELIILAPGLRSFGEDAQ
                     IDAVIRSFGYRPSAEIQGLVAREPALSQNLSAAAHLIHGSSEGRFTISYAPGPAMTRE
                     EIESVGYRYMDLEKAKSRYLPEGRLAGAGLYSTVDGEEYFFVPNPALGLWSTAERMAG
                     VKKP"
     gene            complement(21612..23051)
                     /locus_tag="PK105_02940"
     CDS             complement(21612..23051)
                     /locus_tag="PK105_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU60696.1"
                     /translation="MKLRLRTQFIILTMAVTLFPLVFGLLFFTGQQTKRDPRVTTRFF
                     IDEIAKEWSLDNNISLEDIRSIGARVGLPIMDAALIEPNGTVRVSSFKDLPAGSKIDV
                     MDIARPPPYGKPRPELRFLPIDASLEESPLLIFDIQPFLSREDIRNRNFMLLTSFTLG
                     AFIVAGFMSFLILRSTEKAINNLIEDTAIVAGGKLDHEVKGSGALELHSLADSINHMR
                     ITLRDMISRRMNMLIGVSHDLKTPIALIQGYTDALSDNMASDEATRIHYLEIIREKSA
                     QLEDLVSELIEFMKIDDLNLPQEVVDLWPLLMSLGKRFEEDAHLLGLELQYGFGHFGS
                     ALSKEEPKDLPGVRMNRPLFERAVENLISNAFRFTGAGGHVELALDMKNGQPVLIVAD
                     SGPGISKDDLPYIFDAFYRGTHSRKEAGHGFGLTIVKSVVDLHGWSIEARNKKSPAQS
                     QGENHAQEGGKDQGLVIEIVMKPAPAAHD"
     gene            complement(23048..23755)
                     /locus_tag="PK105_02945"
     CDS             complement(23048..23755)
                     /locus_tag="PK105_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU60697.1"
                     /translation="MHGRILIVEDTKELAELYKLYLQNEGFECKLAFDAESALPIAQR
                     EDWDLIVLDLNLPGMDGFQFLEQLRNTKSMPVMILTAREADEDVIFGLGVGADDFVTK
                     PCPPKVLAARIRAHMRRTAKLQMRQNGSIVRFGPYELDLEGMYLTKNSEPISLSAREF
                     NILKELVSNPGRPFTPEDLYARVWGQEYGDISAIGVYIQRLRRKLEDDPTNPYYIQTQ
                     YGMGYRFNPEAFVEKAK"
     gene            complement(23826..>24129)
                     /locus_tag="PK105_02950"
     CDS             complement(23826..>24129)
                     /locus_tag="PK105_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60698.1"
                     /translation="GSVYALANFSDGSLRFNPQLKVQVGDEGGKVSFTISDLTSIGDV
                     GSEYAGKGNMTTPALTVSLLNSEVSLQASVPISLNADYSVKKVQTKFSVIWNAIEF"
BASE COUNT         6504 a         5785 c         5304 g         6536 t
ORIGIN      
        1 attgacttag atcgtttgtt cctatcgaga agaaatcaca agatcttgag agtacgtcag
       61 aacagatggc cgcgcttggt acttcgatca tgattccagt ctctatgtct tcgtctatct
      121 tgtagccctt tgatttgcat tcttctttcg cttcggccag aacagcttga gcacggatca
      181 gttcatcgat gttgctaatc ataggaaaca tgatattgac tttcccctga cctgaggcac
      241 gaagaattgc tctaagctgt gttttaaaga gatctgtctt ttcaagacaa aaacgtatag
      301 ctctccagcc cagcagggga tttttttctc ctatgactcc aagttccggc aatactttgt
      361 cgccgccgat gtcgagcgta cgaataacaa caggcctgca atgcatggat cttgccactt
      421 gcgaataggc attgtactgc tcttcttctc ccggtatatg cccaccaaga aagaagaatt
      481 cagatcgaaa cagtcctatc ccctcggcgc catgctctat ggcatttaca acttcttcag
      541 gcataccgat gttagctagg agcatgatgc ttgtgccatc tttcgtatga gccggaacat
      601 ctcttagttc tctgttctct ttttcctgat tgaggcgagc ggcacgcgcc gatttctcac
      661 tctggataat cgaatcatcg ggctcgataa caacagttcc attatcgcca tcgatatata
      721 ccgattgacc ttgtctcact tgcaggataa agcctgggcc tacaccgaga acagcgggta
      781 tttcgaaggc tcttgccagg atagcggcat ggctcgtccg gcttcctgtc tctgttacta
      841 tgccccgaac catgctccgg gacatcatca ccatttccga tggcaagagg tccttggcaa
      901 cgaggattat ctcggtttgg atgtcggaaa gcgaaatcct ctcttttttt aaaagatgac
      961 ctagcaaacg ccttaccaca tcatgaatat cggatacttt ttcttgctta agaggatcca
     1021 gcgatgaaga aagcctattg accatctcgc tctcaaaaga gagaatcgcc acttccacgt
     1081 tgaactgctt ttctttgagc gaacgctcta tttgttcaag aaactctgga tcgtccagca
     1141 tcatgatatg cgcatcgaaa atggctgctt gttcttcgcc tgcttcattc tttgccagtt
     1201 ctctcagcgc ttcaatttcc tttttggcta gcaacgatgc ttcatgaaaa cgagccattt
     1261 cgcttggcac atcatcgatg gctatatggt actgaggtat tatgccagaa tcatctatat
     1321 gcacaaaggc aggagctata gctacaccgg gagaaacgcc tattcccttt atgatcatca
     1381 tgatgaaaca atgtatcaga tatttgtata tgcgtcaaat aagacgttcc cagattctcg
     1441 ataagcctct tttcgagagc atgtgaagat caaggtaggt aattcaaggg attgaccaga
     1501 accccatttt ttataacttc aaagtgcaca tggggacctg tgctataccc ggttgtgccg
     1561 attctaccta tggttcgacc ttgagagaca tactgtcctt cctgaaccag aatttcggac
     1621 atatggccat ataatgtctt ccatccgccc gaatgggaaa tgatcgcata tttgccaagg
     1681 atcggcgaat aacccgtctc tattaccctg ccgtccatcg gggcggttat cgacgagcca
     1741 tagggcgccg ctatatcgat agcgttgtgg aacgatcttc ttcctgtaaa ggggtcttta
     1801 cgccagccaa accaggaggt aagacgacct ttgaccggcc attggaatag tgtgccggcg
     1861 atttctcgca atgtggctgc aggtaatttg acatccggaa gaaacagagg tttgcctgct
     1921 ggaatcgtct ctgaaagcag accatttgtt tctatgagac ggtcgatatt gatattatat
     1981 gaatcagcaa tttcctgaat tgttgttggc tttgaagcga tatacaaaat gccagattcg
     2041 ctgggtatct tgaggagctg acctggcttt agtgatttgg ctgatttaaa attatttata
     2101 ctgataagtg tatccagcgt cagatcaaaa tactctgcta tctctgacag cgtatcgccc
     2161 ttctttactt catagacaga atattttacc aatttgtctt ctggttctgg catagcaatc
     2221 tcattgcttt ttgcaaagtc actgacgtcg gaacctccac gcgcggtcat gggacctgga
     2281 gctggcctga agctcgatcc aagcaacata agcatacatc caacagaaaa caccagcttt
     2341 cctaggttcg atccccgccc tatatagtat acccagttgc tttgatccag cttgattcgc
     2401 attcttatcc ggaacctctc aatctgatga tcaaatatta taagtgcaac caatagaatt
     2461 aggcaagtgc aatttattca tcttgatttg ccttgatgga agcctgtggt ttctctatac
     2521 tctggtctca ggaatattac atgaagccgg aaagagccga tgtcgcactg atcagatgtg
     2581 agagttacga agccgaaaag ctggataaaa cgattgaaac atgcgcagag attgcgagtc
     2641 ttcctgattt acaaggcaag acaatcctca taaagccaaa tgttttgaat gcttcgccag
     2701 cttcaaaggc ggttacaacc aatcctgcct ttgtcgaagc ggtcattcga tttgcaaagt
     2761 ccaggggcgc aaaagaaatc ctggtgggtg attctccagg atggcagcca gtcatgttag
     2821 ctgcaaagac ttctggtatc tacgatgcgg tccagcaaag tggtggtacc tgggcggatt
     2881 tccgagaggt ttctccgcat gctgtcaaag agggcaagaa gctcaagaac atccctctga
     2941 catcactggt agaggaggtt gacttagtca taaatcttcc aaaactgaag aatcatggcc
     3001 ttatgacata tacgggcgca atcaaaaacc tttttggact tatccctggg acagcaaaga
     3061 gtgcgatgca tcttcaatac cccgcggtta tcgattttgg cgagatgctt gtggatctgg
     3121 ctctatctat tcctaattgc tttactttta tggatgcgat catcgcaatg gatgggcaag
     3181 gccctggatc tggaacaccc tactcgcttg gcattgtcct ggcatctagg agtgttgcaa
     3241 agctagactg gattgcgtct cagtgtgtag gctatgaccc tagaaatata tcctatattc
     3301 ttgatgcaat ggaaagaaca tctggaagcg caaatattac ggaaccttcg acttaccctc
     3361 taagagtctc ggaaatagag catagtggtt ttaggcggct tccttataca agcaagcttg
     3421 gcaaaagact cagcgcaata ccgagaggcc ttcgttcctt agccgggaag ctgatacacc
     3481 ttaggcctat atttcataaa aataagtgca tcggttgcag tgcgtgcatc gagatatgtc
     3541 cagcaaaggt attattgctc gataggaaaa atggctcgaa catcattaga atcaatgaca
     3601 agctctgtat cacttgcttt tgctgccatg aggtatgccc ggccaaggca attactgtag
     3661 gacgcgcgcc ggtccgtatt gtgcatcgac gcttgcctgg cctttgatat gagaagctat
     3721 ccatggctag caaagccggc aaaggaggaa aaagagtggc tcatgtcagt cttgatggtt
     3781 taattcgctt tgggaactta tccgatttgg agactatgga tttgatcgct cctatggaaa
     3841 agaatggcta tttggtactt caagcagatg cgcttcgcga gtgctcccga attgcgttta
     3901 cgaaaatagc ttatcttatg cctgaaacgc aatatgatgc ttttgctaag atcatcgagg
     3961 atcctgaggc tagcgaatat gatcgatttg tcgcatcttg tcttttagaa aatgcactga
     4021 tttctggaaa gagaagatat ccgatatgcc aagacaccgg gaccgcgctt gtctatggat
     4081 ggcgcggtga ccgcatagaa atcgagaaaa acattgactt ggaagaatta ctcgttcaag
     4141 gcgcagcaca agcctggacg caggagaaat tgcgcaactc tcagatgggt cctctaaata
     4201 tgatggaaga gtgtaataca aaggacaatc tgcccgcagg tatccaaatt cgttctataa
     4261 aaggaaagga acttaggctt acctttgtcg caaagggagg aggctccgct tctcgctctt
     4321 cgatcagcat agagagcccc gctattctga gagcagataa actggagaat gttctgcgtt
     4381 tgcgcatcaa tgcattgggt ccttccggct gccctcctta cgcgattgcg atggtgctcg
     4441 gcggtacaaa tcctgcacag tctttgtatg ctgcggaact cgcggcccta ggcttactgg
     4501 ataatcttcc tacctctgct aatggcgatg gtatgcccat gagagatatt ctctgggaaa
     4561 agaaaatgct gacaatagct cgggatagtg gatttggggc tcagtgggga ggttcgcatt
     4621 ttgccgtctc cacaagagca ataagactgt cacggcatgc ggcaaatcta cctttggcga
     4681 tcgcagtgtc ctgcgcagca aatcggcatg tctctgtatt tatagataat tcgggatggt
     4741 atatcgagaa actatcacct ccttcacaat gcttagaaaa taaggcacaa gagatcattc
     4801 tgcagacaag cgccaaggcc gagattatac aaataacgca atcggatgat agctggcttt
     4861 caaaattaag gtcattgcaa gcgggtacga tggcccttat ttcaggacct gttcttttgg
     4921 cgcgggatat ggctcatgcg cgccttttgc gcatgctcga gtctgacaaa aaacttcccg
     4981 attggttcat cggattaccg gtattctatg ccggaccaac agaggctaag cagggagcca
     5041 agactggggc ctttgggcct acttctgctt cgaggatgga tagctatctt gaagctttta
     5101 tgaaagtcgg tgcttcgatg gttacaatcg caaaaggaag ccgtagcaat gaggcgcgta
     5161 gagctatagc cactcataga ggcgtatact gcgctgcaat cggaggagta gccgcgctca
     5221 atgcagagcg cttcttacaa aaactggata caattcagtt cgaagacctt ggaatggagg
     5281 ctgtccgtat cgcagtgatt gatagactgc ccgtcattat agctatcgac tcacatggaa
     5341 aagacatcta tgattcctag gtctcgctag gctttaatcg ctaggcttta agagagccaa
     5401 ctaatctgtc tgcaaggtat agggctgtca ccataagagc gtgccttaac tccccttctc
     5461 cgatgcgacg tcgtacttca tctgcatcta caaggtaagc gtcgatgatt tcattttgat
     5521 cgaggtgccg atcgactgca ggttttggat ccagcgcaac aaaaacatga aaatgattat
     5581 cctgaatcgc agggtttgga aatacggtac ccgcatgaag gatcttatcc gaaacccaac
     5641 cagtttcttc agctagctct ctggcagccg catgagaagg ctcttctcca ttctccacca
     5701 cgccgcccgg gaattccaag ctaagctcct ccgctccatg ccgatactgc cgcaccatta
     5761 aaaacgaagt gccggcatct gttcgcacat agggaacgat caccgcccaa tcttgggctc
     5821 tcatcaagga aaaatggcct gtccgtccat cggggccttt gctttctctc tccaatacgc
     5881 tgaacacatt gcattcatag actttgcgag tggatatttc catccatttg cgtccaccat
     5941 ttccatcgcc tgcaaccata ttattctccg ctagcatcct attctggatg gcttagatgg
     6001 gcaagctctc tatgaaattg tgcaactttc ccttgtcgaa atttcgcgag atccttataa
     6061 tcaataaaat gaatatcaaa ccctgcaacc gattgcgcaa taaggctctc agggtctttt
     6121 ttcttatggg cgcaggcctt gcgctatttg gtcagaacat ccctaagagt ttatctatgg
     6181 agaaacttat catgcaatct tatcgtccta tcgtcattgc tcatcgcggt tttagaagta
     6241 ttgctcccga aaatacactt ctcgcagctc agaagggttt tgacataggc gccgatatgt
     6301 gggaactcga tgttgcggca tcaagcgatg gtatattggt cgttttgcac gacaacactc
     6361 ttacacgaac gacagatgct aagactcgct ttccatctcg ttcgccatgg attatttacg
     6421 attttacgtt tgatgaactc aaatccctcg acgcaggctc atggtactcc aagacagatc
     6481 cttttatgca aattgcctat ggaagagttg ggtcttctga gcttgaatcc ttcaaggatc
     6541 taaggattcc cacgctgcgc gaagcgcttg aattgacgaa gcgtctgggg tggcgagtga
     6601 atatcgaaat caaagatgca accggatttg cctgcgatgc ttggattgtc gagaagacag
     6661 ccgaactgat aaaagagctc gatatggttc cttcagttct gatttcctcc ttcaatcatg
     6721 attacttaaa gcgaatgaag atggcgatcc cagagataag tgttgctgcc ctaatcgata
     6781 agccaatctc agaccctgtg cagagattga aggatatcgg ggcggtggct ctcaatccta
     6841 acgaaaaata cctagatgaa gcaacggtca gagcattaag atccgcaggg cttggtgtgc
     6901 ttccatggac cgtaaacgat ccttcaagaa tgagaatgct tctgaattgg ggcgcgaccg
     6961 gacttataac cgatttcccc gatagagcct tggaattggt caagtgagat tgttagagga
     7021 ttcctagatt tgttttctat tgcgtgctca aaaaaactat aatataatat tttccgttaa
     7081 gaatccggaa gcagatccgg caatccataa taatcctgga ggcagagtaa tgaaaagagt
     7141 cgttttgctg atagccgttc tactaatggc tgtcggagct tatgcccaga cgacggtgga
     7201 attatggcat gccatggccg gccataatgg cgaaatggta cagatcatca gtgatcaatt
     7261 caatgcaagt caaaagcaat tcaaagtagt ccccgtctat aagggatcat atgtagacac
     7321 aatgaatgct ggtattgcag ctttcagagc tgggcaagcg cccgcaatta ttcaggtata
     7381 cgaagtcggt acggctacca tgatggctgc caagaatgcc ataaaaccgg tctaccagct
     7441 tatggctgag aataaaatta attggagctc cgatatctat attccggcca tcaaatccta
     7501 ttattccact tccgatggcc gaatgatttc gatgccattc aacagctcca ccgctgttat
     7561 gtattacaat aaggatgcat tcaggaaggc agggctggat cctgaaaaac cacccgttac
     7621 ttggcctgaa ttcttcgagg ttgcaaagaa actaaaggct tccggcatgg aaggtggttt
     7681 tacgaccaac tggataagct ggattcagct tgagaatttc tgtgcatggc ataatattcc
     7741 atttggaacc agaagcaatg gattcgatgg cctggacacc cagcttgtat tcaatagtcc
     7801 tatcgtggtg cgccatttcc agaacattta cgacctttcc aagaccggtg tattcattta
     7861 tggcggccgc gaaaataaag ccaatcccct cttcacctct ggccgcgttg gcctgcactt
     7921 cgagtcgatc ggtggctatg ggaacatgaa ggcaacctgc aagttcgact ttggtgttgc
     7981 gcgcttgccc tattatcccg atgtacccgg tgttccccag aattcttgca ttggcggtgc
     8041 ttctctctgg gtattcaatg gcagacccgc agcagaatac aaaggagttg cagagttctt
     8101 caatttcatc tccaagcccg agattcaagc tctttggcat aaagaaaccg gctatctgcc
     8161 tgtcacccaa gcagcctatg agcttacaaa atctcaaggt tactatgtca acaaccctgg
     8221 actcgaagta gcaatcaagc agctgaccaa caagacacct actcctaact cgatgggtat
     8281 ccgctttgga aatatgccca taattcgcga aatcgaagat caggtttggg aagaaatcct
     8341 tgccggaaaa atctcggtaa aagatggcct cgacaagatg gtacgtgaag gaaacaaaca
     8401 attgcgcagt tttgagaaac ttaataaata gatatttgat tccgcactgt gaactttaag
     8461 aatctcagag cggacaagaa ggagatgttt ttgtaataaa aggccgccgg tcggcggcct
     8521 tttttgcgca gatatagaaa aggactgcgc gctatgcaac ttctttgtgg tttagtataa
     8581 taatctaaat tcttcaggaa ctgttatgtc caaacaatat gagttcagag cttcaatttt
     8641 gccgtatctt cttgtgctac cacagatgtt aatcgtcttt atctttttct tctggccttc
     8701 tgcacaagcc attctgcaat ccttctacct gcaagatcct ttcggtggca agatgatctt
     8761 tgtaggtttc gagaattatt ccaatcttct aattgatccc gattattggc aatctttctg
     8821 ggtatcgaca gtatttgcgc tatgcataac cataagctca atggctattt ctctttttct
     8881 tgcaatccag gcaaacaaga aaatcagatt ggcttctttc tataaaacaa tgctcatctg
     8941 gccatatgct gtatcaacac tcgtcgctgg agtcatttgg cttttcatgt tcaatcctaa
     9001 cgtgggtgtc atcgcttaca cgcttaagca taccttccat atagattggc agtatctttt
     9061 gaattttaac caagcctttc tgttgctcac catggccgcg agctggaagc aactggccta
     9121 caattttgta ttcttccttg caggactaca gagcgtcccc gcaactctgg tcgaagctgc
     9181 ggagattgac ggggctgggc cgaccaagag attctggaaa atcatattcc cccttctttc
     9241 gccaactact ttctatcttt tggttatgga tctggtatat ggattcttcg aaaccttccc
     9301 tattgtgcat tccataacat cgggagctcc ggggaagtcc actgctatac tcgtctacaa
     9361 ggtttggcga gatggcgtta taaaccttga ccttggaagc tcttctgcgc aatcggttat
     9421 cctgatgata atggtcattg ttctgacggt attccagttc cgcttcatag agcggcgagt
     9481 cacgtactaa agcggaggaa acaacaatga atgaacaaca gctatctaat aaagccgctc
     9541 accgagccaa ggtccttcgc agcctttatc cacatatcat cctctggatt ggcatagtta
     9601 ttatcatatt cccgatttat tttattttct cggcttcatc tcacacaaat gcagatatac
     9661 aagcagctcc catgcctctc ggcataggac ctcatctaat agagaattac cgccaggcat
     9721 tattgcatgg aacgcagaac atgggcacac cggtccttac catgttgaag aattcgctta
     9781 taatggcgct tggtatatct attggcaaga tagtaatttc tcttatggca gccttcgcaa
     9841 ttgtattttt cgactttcca ttgaggggct tttgtttctg gagtatcttt atcactctga
     9901 tgttgccggt agaagtgcgt attatgccta catataaagt agttgcagat ctgggattgc
     9961 tgaatagcta tgcgggctta attctgccaa tgatcgtctc agcaactgct gtatttttat
    10021 ttaggcagtt cttcatgtca gtacccagag agatagcaga agcatcgcag attgacggcg
    10081 cgactcccat gcagttcttt agccatatcc ttgccccgat gacacgtacg ccaatcgctg
    10141 caatgtttgt gatccaattt atttatggat ggaaccaata cctgtggcca ttgctaataa
    10201 ccactaaacc ggaattctat accctgctaa ttggcataaa ccgaatgatg tctggtgcag
    10261 acgtccaaat cgaatggcag atagtgatgg cgaccactat gctagccatg cttcctccgg
    10321 tcatagtcgt agtgctcatg cagaaacagt tcatagcggg catgaccgaa acagagaagt
    10381 aatctcagag gaattactta gtcaacacga tatggcaaac cagatatatt cctaagaata
    10441 acaaagggct gccctagatt gatctggaca gcccttttga ttttttatcc tccgaatgcc
    10501 ctgacgccga tttcaaagca tttcaaaata attaacagca ttctcccaac aaatgccacg
    10561 cacaagctgt tctgtataga gtggattatc cggcagctct ccagcttcga cccattctcc
    10621 gattatattg catagaagcc tacgaaagta ttcatgcctt ggaaaggaca taaaggatcg
    10681 agaatctgtg agcattccaa cccacgcacc cagtaatccg actccggcat actccttcat
    10741 atggcgttcc ataccatcct tctggtcatt gaaccaccag gctgcgccta gctgaacctt
    10801 tccggcgacc tgtggcgaac taatctcagg agcggtgcct gcaaagctac ccgcaagtac
    10861 agccagagga ttatgttttg aagggtctag cgaataaaga atggttttcg gaagatttcc
    10921 ctctctctct aatgtctcaa gtaaagaagc aagaggagca ataataggcg catcggaagt
    10981 gcaatctcca cccacatccg gaccaaacac cctattcaat cctggattta tattcctgag
    11041 agctcctata tgtagctgca ttgtccatcc cttttttgca tttagcctgc caaaatgcaa
    11101 aagcagtgca gtttcaagct ttgcgcgctc tatctgcgat agcgctattc gacttcggcc
    11161 tttctttaca atgcaatcaa gctcggtatc gatagcttgc acaaaaggcg gaacaagaag
    11221 agcatgatcg gagagtctac ttcccataga gtgaaagtaa gcatgccgac tggccaaagc
    11281 atccgcaaga cttgcaaaag aatcaatttc tatgccagcc gcttcgccaa gcgaatcgat
    11341 atagacattc caagtctcga tatcctcggt tttcatggcc ttgtcgggac gaaaagcggg
    11401 caatatcttg gtcttgaacg aagatcccga tagttgctcc tctgcaatcg ctctatgcca
    11461 agccaatgta tcgacaggat catctgttgt acacaatacg ctcaccttaa agtgttccag
    11521 gagagaacgc gggctaatgc tcttttcgga aagaattcga tttgccttgt ttcttatact
    11581 caatgcactt tgcggtgtga gcacttcatc gatttcgaat attcttttga gctctagatg
    11641 agaccactgg acaagaggat tgccagctgc cttagtgaga gcgccagcat aagcaaggaa
    11701 tttttcatcc caagctccgg cacctgcaca gagcgattca ggcgcaccat ttgctctcat
    11761 tatgcgccat ttgtagtggt ctccttccaa ccaaatcttg gctaaatcat cgaagggctt
    11821 gtcttctgca atggctttag gcgacaagtg gcaatgataa tcgaagattg gtaaatctcg
    11881 tgcaatctcg aaaaagagcc gctttgcagt ctcattcttc aaaagaaaat cctcatcgag
    11941 aaaatcagcc atatgactcc cccttttatt ttgccgttta aattaccgtc gattgagata
    12001 ttacagattg aagtattgta gttctaaatg ttgtcgatga ggaatcctcc atcaatggga
    12061 atgctcactc ccgttacgaa accagatgcc ttcgaacttg cgaggtataa cgcagttccc
    12121 caaagctctt cgtattctcc aaaacggcca aatggcgtgc gtgcaattat cgcttgccct
    12181 ctcggcgtta gatcgcctgt ctcttcatct attagaaggg cacggttctg attaccaata
    12241 aagaacccag gagcaattgc attgactcga atgccataag gagcaaactc ctttgccagt
    12301 ccttgtgtta gattcagcac gcccgccttt gctgcatcgt atgcccaggt gtcggacaga
    12361 ggcttgtagc ttgtcatcga agtcatgttg atcatacagc cctttatgcc tgcagcgatc
    12421 catcgctttg caaagacctt cgatggtatg accaaacctg ccataaggtt gagctttagc
    12481 acttcctcga agattttttc atccaattca acaaatttcg atctcccaat atttccacca
    12541 acgccgttga gaagaatatc aggtaagcca aaactatgga ttgtttgttc caaggcctgt
    12601 tcgcaagcgt ctgtagagcc agtatcgacc gtgataccaa tgacatcaga gcaatctata
    12661 ggcaaatcag cttgctttct gaccatccta gcggcatctt ccatgggatg atgcgttcca
    12721 cggccccaaa gaactacttt tgctttagcc ttggcaaagg caacagcgat ataagagggt
    12781 agcatgcctc ctccacccgt tactacagca attttgcctt ccagactgaa catcgattca
    12841 ataaacgaca tcgacaactc cttatatttc aatataaact aaagctttac cgaagtattc
    12901 cactttactg gccaggcatt atgagattag gcagaaacat cgtaatctca ggaacatagg
    12961 ttactaagaa cagtgttgca atcattgcaa tatagaatgg aatcatcgcc ttcgtagcct
    13021 tttctattga tatcttgcca attgcacatc ccacaaaaag ggcagagcca acgggcggcg
    13081 tacataagcc tatggccaga ttgatcatca acattattcc gaaatggata ggcgacattc
    13141 caaatttacc aatcaataca ggatacagta ttggcgtgac aatgagaatt agtggcgcca
    13201 tatccataat cattccgagg aagagaagaa gcacattgac aagaaagagc aatagatact
    13261 tgttgtccgt gatagaaagc aaaatctcag tggccatggc aggaattcgc aagtaagcca
    13321 tgagccaaga aaaagaacta gccgtagcaa tgagagtcat gaccattgtg agcgtcttga
    13381 gagtactgta gagaatgctt ctgaattctc tgagtggaat ctgcttatat acaaagaatg
    13441 taataataaa tgcatagact gcggcaaccg cagcagactc tgttgcggta aaaacacccg
    13501 caacaacacc gacaattatg atcacggcta ctaacaatcc ccaaaaagca tcgatagcaa
    13561 tcttgatgcc ttccttaaag gaatactttc tccccttggg atagtttctt cttaccgcaa
    13621 taaaatacac aataactgca agcgaaatac cgagtaaaat accgggaata tagcctccaa
    13681 ggaaaagcct ccctacagac acacctcctg cggccaccga atagatgatc atattgtggc
    13741 tcggagggat tattataccc tgagttgcgc tcgtaacagt gatagcaacc gaaaagtcgt
    13801 tgtcgtagcc attcttggtc atcatgggaa tgataatgac tccggttgag gcaacatcgg
    13861 ctaccgctga gcccgagatg cctccaaaca acatgctatc tactacattg accatgccaa
    13921 gacctccgcg aactcttcct accaggacat tcgcgaggtt tatgattttt tccgcaatgc
    13981 ctccccttgc catcatctcg ccagcgatga taaagaacgg aatggcgagc aaagagaaag
    14041 actttacacc ttgtaccatc tgttgcacga tggccatgag cggtatatgc aggtagatgg
    14101 cggttgcgat agaggctgcg gcaagcgaaa aagtaatggg tactctcaac agaagcagaa
    14161 ggacaaaccc tcccatgagt attatcgttg gaatagtcac atccatagcc ctagctcctc
    14221 aatacgtccc tgattcttat gccttgacca gaaaagtatg cttctaattt ctcatcgaac
    14281 atgttgaacc cgaaaacatg aaaaacagct tcaacaattg ccacaaagcc tgaaagcggc
    14341 aaaacaaggt agaggattcc tgccggcagc atagttgcgg gcataacgga actcatagta
    14401 aatcttacta acttgcttcc atatataatc ataaccgcgc cgataaatat gtagatgatg
    14461 tccgttaaaa tatccagagc gcgatttaga gaggcggaga ttttttcgcg gttggcgaga
    14521 tttatggaaa aatgcaggcg gtactttact ccaagcccca aagaaaggaa aataaaccag
    14581 acttccagca caagcgcaac ctcttccgac caatagatac cgctgttaaa cacatagcgc
    14641 agaaccacgt ttacaaatac caataagagc atcgccacaa tcatgatcat tgatagattt
    14701 ataagtagaa tgtgaatcaa tgtaactgcg gagacaataa attttttcat gataaacctc
    14761 agcttatagg catagaaaac tatatcgaaa tgcgcgcaaa gctacatcta tgctcgacaa
    14821 accctgatgt ctaaccttgc gcgcatctcg actgtttcac cagcggaatt cgatgtttac
    14881 tttaccgcgc gaattctagc aacaagatct tgaatctctt ttgattgctg cttgtacata
    14941 ggctccaccg ccttcatcca ttctgtcttg tcgggtatag ggatgatggt gcaacctgca
    15001 gccttgacct tatccatcga aaccttgaca taagcatccc attgagcgcg ctgataatcg
    15061 atagcatcca tagcggcctg cttaattaaa tcctgatcgg ctttcgataa tctccctaaa
    15121 gaaatcttcg aaccaataat gatctccggg accatggtat gctcatccag agtatagtat
    15181 tttgctacct cataatgact tgcagaataa tatgatggcg ggttgttctc tgctccatcg
    15241 acaactcctg tctgcagacc agaatatacc tcgccataag gcattggtgt tggtattgcg
    15301 ccgaacgaac gcactagccc cagcattagc tcgctctctt gcaccctgat cttcatgcct
    15361 ttcagatccg aaggcgcata taccggcttt ttgctattgt aaaaactcct cgctcctgct
    15421 tcgaaccatc caagaccaat gaagccaaaa ggttcgaggc ttgcaagaag ctccatgcca
    15481 atattgccct tcaatacttt ccacatatga tcagagtctc tgtaaaggta aggcatctgg
    15541 aacgcattga gttttggaac aaatgcagcg acagggctta tagatacgcg agtcatgtct
    15601 atggctccaa actgcacctg ctcgatcaca gccttttcct cgccgagctg agaaccggga
    15661 tagacttcaa ctataatccg accatttgag cgttccttta caaggcgagc aaattcctca
    15721 tcgcccattt ctgtggggta gcctttgggg tgagtttcgg caagcctcaa tacaatcggc
    15781 ttggcttgag caaacaacat tgatggcaag agaatgagca acaaggcaag cgcaataata
    15841 ccgaattttt tcatacgcac aacctccttg agaattcaat cttttgcaga tattagagat
    15901 atacctgcaa ctcattatta taagaagcac tttttagctt tttgtctggt aattccgtgc
    15961 tgattattag caatatggtg cttgagaaga acctagttag atctagattg gaattgttct
    16021 atatagcgag atggcgagat accttcgatt ttggtaaata cttttgtgaa ataactttga
    16081 tcgcggaagc caacgagaaa tgaggtcact tttacggaaa tgccattcaa gagcaggctt
    16141 ttcgctcgtg ctattcggat tctggagagg atctccccaa aactaatccc cgtctccttc
    16201 ataagtaggc gagaaagatg ggaaggactt atattgatgg ctttagccaa atccttgaga
    16261 ccgatgttct ccatgaaatg atcctcgatc cacaacaatg caatacttgc tattttatga
    16321 gtgtccttgg cttttggctc atcggtttgt atcggattca atacgatgat gggtcgttct
    16381 acagagctaa gattgaaagc ggttgtagcc atcctcaggg ctatcgatgc cgaacgatag
    16441 tccgctgcac tgatacctat cctaagcgtt ttagctcctg catctcgaca gcggcaaagg
    16501 aagcgcccaa tgctttggtc ttcaaaatgc tcactgtatc tatacaaaaa gagctcttgg
    16561 aaaatcttat tgccctcttc tttttgtatc tcatggggga tgatgatcaa tacattaatt
    16621 atgggatccg gcatttgctc atctgccatt gcttccattc tcaaccctct gggtgatatg
    16681 aataacattg gattggcttt tgcaagcccg atatcggaca gtagaaattt ctcagcaaga
    16741 acggggaagg ttctttggaa ttcacatgac gccaaagata atacatcgtc agacgagaat
    16801 tttttcattc ctgcgactag agctatgcag agccaggtct cgcttcccgt ctccagcttt
    16861 actagctgtc ggatgatctc ggtatccatc gtgttttcgc tcagagatgc acagactttt
    16921 gattggagaa agtccttcaa tccagccgtt acgcgctttg cttccgcctg cctctcctct
    16981 tccgccttcc gagcctttat ctcgcgaagc gctagaaata gagcattatg gacttcagcc
    17041 gtcgatgccg gcttaagaag atattctata acaccatatc tcaaggcctg gcgagcatag
    17101 gaaaaatagt cataggctgt caccattatg attactggcg ggttggaaag aaatgatagc
    17161 ttttccgcaa ccttaagccc atcaacgcct ggcatatgta tatcgagaaa tacgatgtcg
    17221 accttctgcg attgtaggat atccaaggct tcgatcccgt ttgaagcttc cattacatct
    17281 ggaatctcat ttgatggtat ggaattcaaa atacggatca tggctcttct ttcgagttct
    17341 tcatcatcag caaccagaat tcgaatcatc ccaccttttc ccccaagggc agagtaattc
    17401 gcacaagagt gcccttctct tttctcgagt ttatctcgaa gcggcagcta tctccataga
    17461 gaattccaaa gcgtgtcgca atatttgcaa ggcctatgct ccttccttcc agcagttcct
    17521 cttcattgac atcattttcc tcactgaaag gaatttctgc gacctgagga aggttgattt
    17581 ggtcaaagct ctgcacttga agagaagccg caatctttgc tcgaacatca gcaaggcgtt
    17641 ctttcgacat gccaatgcca ttatccaaaa accacaatac aagcccttcc ttgcgtttct
    17701 gtgccttaat cgatatgaag ccaccgctga cctttggctc tagcgcatgt ctaactgaat
    17761 tttctacgat cggttggatc atcagacgtg gaaccctagt tttgcgcaaa gaccgcggta
    17821 catctatatt ccattgaagc ctatctccaa aacgcacctg ctggaactcg agataagctt
    17881 gcgctattac aagctcctca tccaatgtag aaagcgcccc gctatccttt atggttgcgc
    17941 gcaataatgt cgccaggcaa attgcaagag attcggttct gggagcattt tctaaaagcg
    18001 cgctacgagc aatggtattc aaggcattga acaagaagtg tggtttcatc tgttcctgaa
    18061 ggtttaaaaa ctgagcctca cgaagtgctt tgcccatgct cacaagagca agctcctctt
    18121 cgtgcagcaa cctttcgagt tctgactttt ccttgagtcc ctccacatac tccctgatgc
    18181 tcattgccat tgtcgcaaaa gaacgtgtca gagtctctac ttcatcgccc gtatgcactt
    18241 ctacaatacc cgcatccaga tcgcctttgg caagcttttc agcttctttt gccagccttc
    18301 gcagaggtgc ggtaattgct tgagttacaa aaagaatgac ccctatggca atggcgcttg
    18361 caaaaatcat ggagaagaaa atagtccgat tcaggatctc cgccttatgt gagagactga
    18421 cataggtctc ttcgccattt ttcataagct cggagagcat tctgttgaga tatatatcta
    18481 tataaccatg aatccggctt gcattgacaa agacctgata atagtctggc gaattcccgg
    18541 ctctcagccc aactgcccgt gagactaaag ggaaataggc atctagacca tagccggttg
    18601 cccgcacctc gaactcaaca tatatcgaaa tatctgcaat tggaagaagc tcgacatact
    18661 gtgaattcaa gagcaatata ccatcataga tagagttgat ttctaccgaa gctggttcac
    18721 gaagatatcg atccgcctga gcatgaaatg agtttagatt gagcctgaat ctatgcacaa
    18781 gcagataatt tgaaagattc ttgctatatt cgcgcgatag attcatcgat tcattctgca
    18841 tgacaagcgc aagcaccaga aaaaagatga tgatgatccc aaagcatatg aggatctttc
    18901 ccataatgcc agggcgatga aagccaagcc cgcccttcga aggaaaatca gccgaagatg
    18961 atcggcgcga gatgacgtgc atatagatta taccttacgt tgcgagaaac agattcgcgg
    19021 cattcttcga gagcatccag atattctcta cccatttcgg ctgcgatttt gaatcccaca
    19081 tgcataagtt gtctcatgct aggctgcatc gtcgatgaat ttggctcatg acgcaaagca
    19141 gcaacaaaag tatccgaatc ccaaccggcc accattttgg ggctgggtag catctgggca
    19201 tcgatttcga cgacattggc atagggagcc gtaagttctt caattcgaag ataggcctgc
    19261 tcatatacgc tctttgcaac cgaaaggcct ttgcctcctg cttcggcaag gccaacaatc
    19321 tcttcaagcc aggtagtgcc tgcggtcttg agatgaacac ccacttcaag ccttgcagtt
    19381 atatcatgga ttcccttgta aagtttgaac ttatcactgc cagaatgaac tgacagcttt
    19441 aggcctttcg gaagattcag atattcggaa gcccataaaa ggactttagc ttcgtcttcg
    19501 aaagcatgga agaattctgt cgcatctccg atataatcga cgccctttag gaatttgccc
    19561 ggaaagcgag gagcaagagt cgagatcggt attttttcgg catccactgc aacaagaatt
    19621 gcggcaattt gtgctgcagt ctgcggttta tcagtttcgt ccatagatag ttccacaaca
    19681 aaagaacctt caggtttatg ttcggcgata tgtcggtaag tttttcctgc ttcttcgacg
    19741 gccgctaaga atttatccgc tacgctctct agatcagcat gctcaactcg gacaggaact
    19801 gagctatctt ccagcagaaa agccgctcga tccaaaaaac atgagcgcga ttccaatgaa
    19861 gcattctgtc ctatgctctc tgccacatcg atggtaaaaa aattgcaata tggcagaaac
    19921 caatcgacag tcttcattgt tatatgatcc gcatctacga accaccttcc gtcccaacct
    19981 gtttttttga tcgattccga tgcagccttc tcttgatcag caggcgtcga gcctatcagt
    20041 acatgttcgc gattactctt gttccaaaca atgtcggctt ctcctccttt ggctcgcatg
    20101 tccttgaaag cacctatctg agcctctcct tcgcgaccaa accggtcgcc tgtgcctatc
    20161 gaaaagcgag aaagttgcag catatgtccc tcccgagatg ttttctatat aatgtcgctt
    20221 gaaagaaagg aacgcaagct ctttttgaag gacggaacaa tcattatgaa catgatgaat
    20281 atagccgaaa aaaggcgaga atctggcatt ggccgattag aactcgaaca attagtcgat
    20341 agaactataa aagaatcagg tgcgctcgat gcaagcaaaa tcttgataat tccccctgat
    20401 atcacgagat ttcactctcg ggcgggcttg ataaccgacc tattggtcga acagcttggt
    20461 tctcgggtgg cggctgtctt gccagccata ggtactcaca catccatgac agaaaatgag
    20521 attgctcaga tgtaccctct ttgcccaaga acgctattcc gagtccatga ttggcgaaag
    20581 gatgtagtcg agctcggtac gataccagaa gagcgcgttg cagagatttc tggcggcaag
    20641 gtcagatatt actaccccat tcagacaaat aaaattcttg cttttgggga tatagacctc
    20701 gttttctcga taggacaggt tgtacctcat gaggtcgctg gaatggccaa tcatgcgaag
    20761 aacatttttg taggtaccgg aggaaaggaa gcaatcgata agagccacta tctaggcgct
    20821 acctgtggaa tggagagcat aatggggcgt atagatacac cggttagaaa tctcttcgat
    20881 gacgctttag atctttctaa gactagattg cctgcaattg tctggattct cactgttata
    20941 ggaaccgcag gagacggctc acttatgcag caaggatatt ttgctggata cgatagaaaa
    21001 tgcttcgaag aggcctgtgc cctatccgcg gaaattaaca tagcgctttt cgataaaccg
    21061 cttagaaaag cggtggtctg gctagacccc agcgaatatc gtagtacatg gcttggaaac
    21121 aaatcgatct atcgcctaag gatggccatg gccgatgaca gcgagctcat aattcttgcg
    21181 cccggacttc gcagctttgg agaagatgcc caaatcgatg ccgtaatacg aagctttggc
    21241 tataggcctt cagccgagat tcaaggtctt gtagcccgcg agcctgccct ctctcaaaac
    21301 ctttcggccg ctgcccatct gattcatgga tccagcgaag gccgcttcac tatcagttac
    21361 gcccctggcc cagccatgac cagagaagaa atagaatctg tcggctatcg ctatatggac
    21421 ctcgaaaagg ccaaatctcg ttatctgccc gagggcaggc tcgcgggcgc aggcttgtat
    21481 agcactgtgg atggcgagga atactttttt gttccaaatc cagcccttgg tctttggtct
    21541 accgcagagc gtatggccgg tgtcaaaaag ccttgatact cttgctcaag gaatatcaga
    21601 agcttcaaga gctaatcgtg cgccgccggt gctggcttca tgacaatctc gataaccaag
    21661 ccctggtcct tgcccccttc ttgcgcgtgg ttttcgccct ggctctgagc cgggctcttc
    21721 ttattgcgag cctctatgct ccaaccatga agatcgacaa cggattttac aatcgtaaga
    21781 ccaaaaccat gacccgcctc ttttctagaa tgtgttcctc gatagaatgc gtcgaagatg
    21841 tatggcagat catctttcga aattccaggt ccgctatcag ctactatcaa tactggttgg
    21901 ccattcttca tatccaaagc aagttccaca tggccaccgg ctcccgtaaa gcgaaatgca
    21961 ttggatatga gattttcgac agcgcgctcg aatagcggac gattcattct gacgcctgga
    22021 aggtctttgg gctcttcttt cgataaagcg gaaccaaaat gcccaaaacc atattgcagt
    22081 tcgagcccca gaagatgtgc atcttcttca aaacgcttgc ctagagacat aagaagaggc
    22141 cacaagtcca caacctcttg tggcaaatta agatcatcaa tcttcatgaa ttcgattaat
    22201 tccgaaacaa gatcttcaag ctgtgcactc ttttctcgaa tgatctccag ataatgaatc
    22261 cttgtggctt catcggaggc catattatcg ctcaatgcgt cagtatagcc ttgtataaga
    22321 gcaataggcg tctttaaatc atgactcacg ccgatgagca tgttcatccg ccgggaaatc
    22381 atatcgcgaa gtgtgatccg catgtggttg atgctgtctg caagagagtg caattctagc
    22441 gcgccgcttc cctttacctc atgatcgagc ttaccccctg caactatggc cgtatcttcg
    22501 attagattgt tgattgcctt ttctgtcgat cggagtatca agaaggacat aaaacccgca
    22561 acaataaaag ctccaagcgt aaagctcgtc aagagcatga aatttcgatt ccgaatatct
    22621 tctcgtgata agaaaggctg aatgtcaaaa atcaagagag ggctttcttc taggcttgca
    22681 tcgataggta aaaagcgcag ctcaggtcta ggcttcccat acgggggtgg tcgagcaata
    22741 tccatcacat ctattttgct tcctgcgggc aagtccttaa atgaagaaac cctaacagtg
    22801 ccatttggct ctatcaaggc ggcatccatt atgggtaatc ctactcgcgc tcctatgctt
    22861 cgaatatcct ctagtgagat attgttatcc aagctccact cttttgcgat ctcatcgata
    22921 aagaaacggg tcgttactcg aggatcacgc ttggtctgtt ggcctgtaaa gaagagtaat
    22981 ccgaatacta gaggaaaaag cgttacagcc atggtaagaa taataaactg agttcggagc
    23041 ctaagcttca ttttgctttc tccacaaagg cttcgggatt gaatctgtag cccattccat
    23101 attgggtttg gatataatat ggatttgtcg gatcgtcctc aagcttcctg cgcaatctct
    23161 gaatatagac tccaatagcg cttatatctc catattcctg accccatacg cgtgcatata
    23221 ggtcttctgg agtaaatggc ctgcctggat tgcttacaag ttcttttaga atattgaatt
    23281 cgcgcgcgga aagactgatg ggctcggaat tcttggttaa atacatcccc tcgagatcta
    23341 gctcataagg tccgaatctt acgatgcttc cattttgccg catttgaagt ttggccgtgc
    23401 gcctcatgtg agctcggata cgagcggcaa ggaccttcgg aggacagggt ttagtcacga
    23461 agtcatccgc tcccacacct agcccaaaga ttacatcttc atcagcctct cttgcggtga
    23521 gaatcattac aggcatggac tttgtattcc tgagttgttc gaggaattga aagccatcca
    23581 ttcccggaag attaaggtcg agcactatga gatcccaatc ttctctctgt gcgatgggga
    23641 gagcgctctc tgcgtcgaat gcaagcttgc actcaaagcc ttcattctga agatagagct
    23701 tatagagctc agcgagctct tttgtgtctt cgacgattag aattcttcca tgcatgcccc
    23761 ctcctctgca aacgtgctcg ccaaataacg tcagaccgct tgcatcgatt ctccatctct
    23821 tttcgtcaaa attcaattgc attccagata acactgaact tcgtctgaac cttcttgacc
    23881 gaataatcgg catttaggct tatgggcaca gaggcctgga gcgatacctc gctgttgagc
    23941 aggctcacgg taagagctgg tgttgtcata ttgccttttc ccgcatactc agaaccaaca
    24001 tcgccgatag aagtcaaatc agaaatcgta aagctcacct ttcctccttc atctcccact
    24061 tgtaccttga gctggggatt aaagcgcagc gaaccatctg aaaaattggc aagagcatag
    24121 acggagcct
//
LOCUS       DAOOWY010000020        23429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2192_length_23429_cov_9.665012, whole genome shotgun sequence.
ACCESSION   DAOOWY010000020 DAOOWY010000000
VERSION     DAOOWY010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 23429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23429
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2192_length_23429_cov_9.665012"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..2325)
                     /locus_tag="PK105_02955"
     CDS             complement(169..2325)
                     /locus_tag="PK105_02955"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU60699.1"
                     /translation="MNRIEHHPILTAKKNLTLVKFSFDGEEITGYEGEPVSSALISAD
                     RHVFSYHFRDDFPQGLFCANGQCSQCTMLIDGIPKKSCVTPLAQGMDVRTIHGLPRLP
                     SQDRPLVQAERRSIRTDILIIGAGPAGLAAAAELGNMGFQVLVADDKQQAGGKLVLQT
                     HKFFGSEADCYAGTRGIDIAEILKEEALKYPSVVILTNAPVVGIYKDRKAGIYLNYES
                     YLLVEFSALVVAAGAREKALVFPGWDLPGVIAAGAFQTLVNRDLVKAYSKILIVGSGN
                     VGLIAAYHALQADIEVTGIFEAAGRVNGYQVHADKIRRLGVPIHLNTTITRAEGCQKV
                     QRAYVSAVDERFQPIPGTARGYEVDAVLIAVGLAPCNEFLEQARRYGIMAVAAGDASE
                     IAEASSAIYGGKTAAANLALMLGLTKKGAIDPGWEITRKVLASRPGDSYERSPIVPGK
                     EWRPVFFCSEEIPCNPCATVCPLGAIELRSMQGNIMDLPYFAGDHCTGCGACVSICPG
                     LAITLVRRVTDDKALVVLPWEFNVDSGLEEDMELVDQKGEILERARIISRRKVQKRNT
                     WLLTFEVDVAHAARIAGVRRLKTQKPARLQEKAIELENSSSIMPVDKSLQEDLDTVVC
                     RCERITLREIVDFIKRHDVKDANQLKSLRVGMGACGGKTCSQLIAKAFSIAGVNPAEI
                     EPFTQRPLFMEVPMGSLVNEGLHKLGGKDAGTRGAP"
     gene            2485..3966
                     /gene="murD"
                     /locus_tag="PK105_02960"
     CDS             2485..3966
                     /gene="murD"
                     /locus_tag="PK105_02960"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOU60700.1"
                     /translation="MDEFRGKYITIMGLGLHGGGIASARFFAKAGAEVTVTDLRDEKT
                     LSPAIHELQGLPIRYVLGRHEIEDFAHADLVIKNPAVRRDSPYLAYAKRIETDISIFL
                     RYSKSPLVAVTGSKGKSTIASAIWHVLFQNNKKTLLGGNITVSPLDFLDKTDAQVPVV
                     LELSSWQLGDLRGTGLLKPSVAVLSDILPDHLNYYDSMDAYVSDKRVIYESQDENCWT
                     VCNADQKWGHAFAEETLAQVLWYSERPLEDSVGKNALGGWLTKSKTKSNYSDDSGPSY
                     WSLDLLEGYGRFSLDGQEELLVPSQVLVPGRHQKKNILAAAVAARAFGLSPEGIMSAL
                     ASFPGVPHRLEFVGSIRGIKWYNDSTATIPDATIAAIDSFSSPIVLIAGGSDKMSDFS
                     AFAQKAKNLKAVILLAGSGTDRLIPILDVNKIAYNGPFFSMHEAVQAADKQADPGDIV
                     ILSPGCASFGMFLHEFARGDAFKREALMLFRQYAEQGSTAQKR"
     gene            complement(3947..4798)
                     /gene="amrB"
                     /locus_tag="PK105_02965"
     CDS             complement(3947..4798)
                     /gene="amrB"
                     /locus_tag="PK105_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOU60701.1"
                     /translation="MTTNRRIRKRYLPSGWYPEDSAEISSLVADWTKSQNNLSAYAVV
                     APHAGWYFSADLAARAVWALRDCDTVAILGGHLGNGEPILYGDEDGFDCTVRDAENDI
                     EVLDSLKVELKNVGIQEMAPDRDIDNSVEILLPLAGFRFPNARILWLRVPPDYKAREL
                     GSALFRAASSCKKQLVAIASTDLTHYGPNYGFMPRGLGEPAVQWVRNENDKGFISAAL
                     AMDADKVLKHARNNFSACSSGAVAAAISFALNAGARHSLLIGHKLSIDIHPDRSFVGY
                     AAIAFVP"
     gene            4917..6278
                     /locus_tag="PK105_02970"
     CDS             4917..6278
                     /locus_tag="PK105_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60702.1"
                     /translation="MKRTLRIILFIGTFVLLALIVGYVAWKIGEMRLLGEQSASAEYS
                     ILRNAASLITSEEDLGDQFVRDRLKSLYNASTHLLAVQVLDRNGLVLWKMPDESPYFA
                     SPATNPGSVFRAPGMSTIIYMTPLPDGMKLMALYSILSQRDISNILLVPAIILAAWIV
                     LLVILQFALKERPIEASEIIGAEKMPETPEKPQYHEEPLESVPYSEEALLTISDEASS
                     IAEESIENHASLENIENIEENIEEEAEEITNKKVEIPVFPQAPQPEPETIPMSISSSD
                     DFLPGEFVAPAQQIEEEAEEAPQPEIFPSTLSLAELERSLDIELRREKETSLLLVHCL
                     LSGELDPSALALGVTIRDYFASDSLVFELGKGCYAVVLPGMDAGSALKVAVDLDDVLT
                     TTASLYKDLSEEPPFYFGLSARYNRTISPDRLYREAFAALKEAQERSGSRILAFKPVS
                     SVA"
     gene            complement(6293..7222)
                     /locus_tag="PK105_02975"
     CDS             complement(6293..7222)
                     /locus_tag="PK105_02975"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOU60703.1"
                     /translation="MNLEAFVLGCGGMMPLPYRQLTSMLIRREGELFLFDAGEGTQVS
                     IRRLNLRWKKITAIFISHMHADHVTGLPGILMLSSQVDREEPLYIFGPPRIAEFVDQN
                     RHLLDMYINYDIIVREIYEAGIIWQSDDYLIRAFPLKHTKMCFGYALEESKRPGVFYP
                     EKALALGVPRGPLWSELQSGKTVRLTERSSIEPSEVMGEPRSGRKFSFVTDTLYFPEI
                     AQEVSNSDLLICEGMFEHALIESAVDKRHMTARQAALIARDAGNIKKLGLIHYSPRYA
                     DRELKILLDEAREVFPDTVLTKDRMRFPIEFVD"
     gene            complement(7340..8491)
                     /locus_tag="PK105_02980"
     CDS             complement(7340..8491)
                     /locus_tag="PK105_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60704.1"
                     /translation="MKRFVGLLCLIIIANILVSCQSSMEEGKIQQLFDLGYGKSDSSI
                     DISVQNKNSVDICLRKGIFHLLSQEEAKILRISSYGQTLAMWYDPKRGSAPLVLEDVQ
                     MSDFSSGKDLGRFAIQVSFEDPGHIASDSRQMLYVSDAEIVRRFDSDGRELPFLGQEG
                     IGGIRFPVITSLDVLSNDDIAVSCSSESGKAVYFYDRNGTFLHVLKWDGQHMPIPVSI
                     AENHPNTGGSSIIASLESFLPCFLNGRRVVMLKINYYYEISDPSSGVTISIEPVGGWI
                     IETDASNGALLNSFPLQSSLEDLGIDQQMIAAQSNGIFCVRWRDKGLSGEVFRFNQQG
                     KINGKLDFQVPDADASLIDMVVGDDGNLYMLTQLSTELKMYTWRILTLR"
     gene            8547..11318
                     /gene="secA"
                     /locus_tag="PK105_02985"
     CDS             8547..11318
                     /gene="secA"
                     /locus_tag="PK105_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOU60705.1"
                     /translation="MSRNTFLSALLGSKEEKDIKAVLPLLHKINELEPWALSLADSDF
                     PAMTEKFKGRLANGETLDDLLPEAFALVREAARRKLGERPFDVQIIGGIALHQGKIVE
                     MKTGEGKTLSSVTAAYLNALTGQGVHIITVNDYLAERDSQWMGQIYKFLGLTVGCILS
                     AMDNIARKKAYLSDITYGTNNEFGFDYLRDNMVWSLDQKVQRGHAYCIVDEIDSILID
                     EARTPLIISGPADDDTYKVNEVNRLASSLFEVKKNPETGDYPDEARGELLEGDFKIDE
                     KSKRVMFTSEGLNHIESLLQKRGLIKGSLFDPENFQFVHYFTQAVRAQHLFSKDVDYV
                     VQDNQVQIVDEFTGRILHGRRYSDGLHEAIEAKERIKIARRNRTLATITFQNYFRLYK
                     KLAGMTGTAETEAEEFNKIYNLDVMIIPTNRPVVRKDENDLVYLNEAEKYDAIIGAIR
                     EIHTKGQPILVGTVSIEKSEKLSALLIKSGVKHEILNAKNHAREALIIAEAGSKGAVT
                     IATNMAGRGTDIKLGGNPEFRARRKAGTEAPEEKYREILSREYETWTKDYEEVKSLGG
                     LCVIGTERHEARRIDNQLRGRSGRQGDPGRSAFFISLDDDLMRLFGGDNFKQLMGKAG
                     MKPGEPIYHPLLSKSIESAQKKVEERNFEIRKHLLEYDDVLNKQRNFIYEQRNSILAD
                     DNLIERVRDAAYEMLEEQNAILSQAFKDNANKAIQEFGSWLFDSFGIEMTTKELGALY
                     KSGGLVSEIKNNLDLDLDAKIQVAGAENLNIFIRFSYLQEIDEKWLNHLESMEALREA
                     VYLRSYAQRNPLLEYKIEGSDIFESLIFSIRHNIASKVFRVRIKESTPTVEATSPRST
                     SRIEAAQTSHEAVSSFVDQSKSVQKESAIALASTPATATVVRQGKKIGRNDPCPCGSG
                     KKYKYCHGR"
     gene            11379..12824
                     /locus_tag="PK105_02990"
     CDS             11379..12824
                     /locus_tag="PK105_02990"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOU60706.1"
                     /translation="MSLNCEEIDLILNEAPLVGMKIQNVYQPTFDSLVLELFGKGILT
                     QYYFSIAHNACRLHPLSTPAPKNERPLRFMECLRSRIRGGTILHAAQISKDRIVKIDI
                     IRANEKGVAEQSYLYARLWSGAGNIILVSVEGIIIDALRRLPARNEVSGAAFVLPQQL
                     DSNKQPSKHYVVRDLEGKGTFWQKIEQFYAQNADNLSRSTLLTQVHDRYERKKTSIET
                     KLNHLVSKQQEYEQAERFRQLGDILLAGYCPEIQGRKAFARVYDYYENKEILIEIDQN
                     LNPAKNAAIYYEKYRKAKSGLEEIRSEIQYWEKSLNHLDAWLNRLEKETNPLIIAKIL
                     RKGGAARSKEKPAFPCQYLDFRGWRILIGRSGKENEDILRHIVRGSDLWLHARDYAGS
                     YVFIKAKKNKSIPPEVLLAAAKLAIYYSKARKNGGGDVHTAFVKNLRKAKEGPIGRVI
                     PYFEKNLYITFTEAEIKEIFQEYQSKEENYV"
     gene            12821..13786
                     /locus_tag="PK105_02995"
     CDS             12821..13786
                     /locus_tag="PK105_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU60707.1"
                     /translation="MKRSKISLVATLLLAVSVFILYASPKDKIAVSVIRGPSGLSSTW
                     MMSELARTSPSEFEFISVAGADMVVAKLLNGEIDAGVLPVNVAAKLYNTGAPIRALAV
                     VGNGMVKFLTTDTKVQSLADCKGKTIYIAGQKATPDYIFQFLCAQKGLQIERDYTAIY
                     SLAYPEIAAGLAGGKISYAVLPEPFASQAILKNPAIRTPIDITKEWQAATDQPDYPMS
                     LFVARKEMIEAYPDKARILLDSYRASINKAIEDPQLIAKLAENLDLGVNAQTAIIAIP
                     KSNFVFVEAYQAIPSIEALLSVFLKFDPASVGGVLPSKSFYAQIK"
     gene            13770..14567
                     /locus_tag="PK105_03000"
     CDS             13770..14567
                     /locus_tag="PK105_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU60708.1"
                     /translation="MRKSSEHRFMLLRWQLLGIISFILLWEIGALLVKSDLILPSPAR
                     VAKALYQLFGKNIFWLSIGGSFLRVFEAFILSGLIGLATGTISGIKPQARAFLNPFIV
                     GIRATPVMALILVAMFWFPAWQVPVFSAILMAFPVMHTSTEAGVHAVDKKLLEMSTFF
                     NVPSSFIFWRLRLPSALPYILSGAKNALGLSWKVVVAGEVLSQPRMALGTGMQEARLS
                     IETATVFAWAIITIFLCGLSEYLFGLLADRLSARFQQSSQEEDQDLS"
     gene            14570..15304
                     /locus_tag="PK105_03005"
     CDS             14570..15304
                     /locus_tag="PK105_03005"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60709.1"
                     /translation="MNIKIDNLRKSYGALNVLEDVTFSLEESRITSILGPSGCGKTTI
                     LNIMSGILQADSGSIIGIEGRKFSYCFQEPRLLGWLSAEDNLRFALSSLFQSSPRTND
                     IELRIERFLREAGLWEFRKFKPSQLSGGMQKRLALARAFAFPADMLLLDEAFSEVDLR
                     KKMELMEDFLHLWKDEEPTVVIVTHDIHDALYLADQVIVLSQRPAIVEGTILIDIPHE
                     KRTFTSSELYGYEKELYRLLGLDRTV"
     gene            complement(15281..17188)
                     /locus_tag="PK105_03010"
     CDS             complement(15281..17188)
                     /locus_tag="PK105_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU60710.1"
                     /translation="MPRVAILESDILIALDISKSIEQEGLAEVDIYEELEQAYQKLNN
                     LEHELFIIDIGDNQPEIIDAAVAIHRQTGLYCLVISDHGVTSIPARLREAQPLGILVK
                     PFSTRELIVNVETALYHSSMEKKLRDSERRYKDLFAYSLSARCVTDIEGRILERNRVF
                     ESSFSNPMKVENVRDILPNDIIWSEIIESLEENQILQREIQTNRISDSFEQRSLDLIC
                     SFSRFREEGSDAQILCEFLDVTESRRLREELFQSQKREEIGRLTSGVAHDLNNFLTSI
                     MGFLEMIKLEIPDDSQINEDIQGIERVVNRASGLTRQLLGFSRHKPYSPAPIDLKEVM
                     GDTYKILKRLIPENISLTLSLPKESLVVNVDISHLEQILLNLVVNARDALEKTENPHI
                     SIQLSQFGQNALYPKEIMMQPADYALIVVKDNGAGIDNENIKRVFEPFFTTKKQNQGT
                     GLGLSIVKSLVEMNEGYITLESEKGRGTTIKIWFPILLETKTTTKLTAQEEYSLLSII
                     NPPNNILKGKHILVVDDDESILEACTRILESAGAIVGACINAGEAILQAERTNFDLLL
                     TDIVLPGVYGNELWDRMKNNGQARACVFMTGYDSYQNEQFAGAALLHKPFDARSLLEY
                     CNNALNSSIEA"
     gene            complement(17245..19170)
                     /locus_tag="PK105_03015"
     CDS             complement(17245..19170)
                     /locus_tag="PK105_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU60711.1"
                     /translation="MKTRQAAILVLFLSLSIAAILSNCSSLPENEPNANSTGDSIISH
                     LDLLVDTDPEQCIAVISNLFEQQREKNQSFDNSIIGEKGIVLFRKAVENIGLLFKKAI
                     ASQDILTARRLSFSADALVQYVSFYDSLGEIVSEELSMIASPAMRLQLALWEAENKFK
                     EHGYVIGKATLSKAHLGEQEEYSFNKIEDFSIENLKSLIETRDPKEYFITWAERAKEN
                     NDVNAEAWFESLSSGQMSYSPSILVSDQDWLADAVSSVVTVYVDRGFKVEGGYSVPDR
                     VLGTAFQISEGLYLTNYHVVQSEIDPTYKGYSRISIRPSDNPQIRVPSKVLGWDEELD
                     LALIQSAEHARSTVMLPPPGMRFHAGDRVFAVGSPIGLENSITAGVVSSLSRKIISFG
                     EAAQIDVPVNQGNSGSPLFSENGVLVGMVFAGLPSFQNINFALPVQWISLSLPDLFER
                     KEVIHARLGLMMGKGSGQSPLVLADLDPSLSAFIAGDTLTSINGKLTKDIASIQFQLA
                     SVPKGALCFLEAQRDFKPVRRLRQISHTDGASLIPAWDRIHKNSILEGLFGARLTQLK
                     ESTKVGGLYSIQWVCPAEPADEIGLSENDVITVYKFRLDEKNKVFILEFSAKSRLSGY
                     FERTIHLEFSTESATII"
     gene            complement(19179..21062)
                     /gene="ftsH"
                     /locus_tag="PK105_03020"
     CDS             complement(19179..21062)
                     /gene="ftsH"
                     /locus_tag="PK105_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU60712.1"
                     /translation="MPDQNDNRDGNKPDLSIPPKGNRAALVLFLSIIVLLVVFLFFGQ
                     DESSREISYSSFLSYLELGEVKSIKIMDQRDIEGVLKGKSGAEMPFTTRIPYFDAELM
                     AKLEEKGVSVTGAISGTSPFQILFGLTPWIFGFIMIWIMMRQMQGNNKAFSFGKSRAK
                     MYSNSNKKITFEDVAGQKEAKYELMEVVDFLKNPNKFLKMGAKIPKGVLLVGMPGTGK
                     TLIAKATAGEANVPFFHMSGSDFVEMFVGVGASRVRDLFEQGRKHAPCILFIDELDAV
                     GRTRGSGLGGGHDEREQTLNQMLVEMDGFDTKDGVIVLAATNRPDVLDPALLRPGRFD
                     RQVVVAMPDVNERTEILKIHMRQIPTSGDVDVEKLSRATPGTSGADLANLVNEAALFA
                     IRKNKELVEMDDFEDARDKILMGVARKSLVIAEEEKRATAIHESGHALLHYFLAHADS
                     LHKVTIVPRGRALGLALSLPDKDTYSRTYGWLYDRIVISYGGYAAEKIVYGETTTGAA
                     QDIKQATEIAQKMVREWGMAEEIGPISLGEEEEPIFLGREIAQHKDYSEATAQRIDAA
                     IQGILDSALDKAINILKREKNRLVRLADKLMQVETLEDKEVRELLGLPEGKILLEEGI
                     APC"
     gene            complement(21067..22539)
                     /gene="tilS"
                     /locus_tag="PK105_03025"
     CDS             complement(21067..22539)
                     /gene="tilS"
                     /locus_tag="PK105_03025"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOU60713.1"
                     /translation="MKTLEYKVKKFLLDMHVDFHKPLAIAFSGGLDSSVLLYILVRIF
                     DHDNIRAIHVCHNIRSKEELERERIFLQKTCKAYSIPITIVNIRQGAISEYAHRKNCG
                     IEAAARHYRYHALVRTAQRFGISTIVTAHQADDQAETFLLRLVRGGRLETLGGISASR
                     LVDSTKGINVIRPLLSFRRYELEAFARENNLEWSEDSTNRELSFLRNRIRHVLIPLLD
                     KEFPSWRSSIESYRWQAQELNTLITSIACQSMPKVMRGLGKDAILDQALFRKEEALVR
                     RRILRLFFMHLGLKDAVSQGALESLERAILQGAPRVEAARCQFDLSNGVVKYIGKANS
                     SSGFLDRSISQLKGLFRFDEFFLRVGEPGQYECGPLKMVVSQSVQPPSLDNASIDKEQ
                     FLRLSIPLSFPFIFRNRKEGENIGDGDRSKDLGSTSKNQKISADISHIVAILEDEYGI
                     AAILTMTFDGRFSIKSDYSSRLLCEKRKILYIFLSRKGDF"
     gene            complement(22689..23345)
                     /locus_tag="PK105_03030"
     CDS             complement(22689..23345)
                     /locus_tag="PK105_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOU60714.1"
                     /translation="MEHIALRVSPRGKLTKGELRQERKKGRLPAQIYGKGIEPISVFL
                     EGKEFANVAKRVTESRIIDLDLEGKKFATLLKEIQKETISGNVIHIDFNLVRHDRNIH
                     VRVPLHLNGVAKGVKDGGILEHSIHEIEVECDSDHLPEKIEVDITDLEANHALHLKEV
                     ALPEGVKLVTNPDTVFAIIKFAHGEPEKALEPEAEEAGEAVASSEQESAETTSEAVHK
                     "
BASE COUNT         6556 a         5443 c         4952 g         6478 t
ORIGIN      
        1 caggatccga gtgagtagct cttattccgc ctatagcagc ccgattctga cctctacccc
       61 aggaagcttc ctcatcgagc aacgcaacct tgagaccttt ttctgcaagc tgccaggata
      121 gcggcaatcc aacggatcca gctcctatta ttacgacatc ataatttttc atggggcgcc
      181 tctggtacct gcgtctttgc cacctagttt atgcaaacct tcattcacaa gagaacccat
      241 aggcacttcc ataaagagtg gtctctgagt aaaaggctcg atctccgctg gattcactcc
      301 tgcaatgcta aatgccttgg cgattaactg cgagcaagtt ttccctccac atgcgcccat
      361 tcctactctg agagatttaa gctgatttgc atctttgaca tcatgcctct tgatgaaatc
      421 gactatttcc cgcaaggtaa ttcgttcgca gcgacataca acagtgtcca gatcttcttg
      481 taaactctta tcgacaggca ttatgctaga tgaattctcc aattctattg ccttttcttg
      541 cagtcgagcg ggcttttgtg tcttgaggcg tcgaacacct gcaatccttg ccgcatgcgc
      601 tacatctacc tcgaaggtca aaagccaggt gtttcttttc tgcacttttc gcctagatat
      661 tatgcgcgcc ctttctaata tctcaccctt ctgatccacg agttccatgt cttcctcgag
      721 tccagaatcg acattgaact cccatggaag aactaccagt gccttgtcgt ccgtaactct
      781 tcgaaccaga gtaatggcaa gaccagggca gatcgacaca caggctccac atcctgtaca
      841 gtgatctccc gcaaagtaag gaagatccat aatgttgcct tgcatcgacc tgagctctat
      901 ggctcccaat gggcaaaccg ttgcacatgg attgcatgga atctcctctg aacaaaagaa
      961 tactggccgc cattctttac caggaactat tggagatctt tcataactgt ctccaggccg
     1021 tgaagcgagc acttttctgg ttatttccca ccctggatct attgcccctt tttttgttag
     1081 tccaagcatg agcgcaagat ttgctgctgc agttttgcca ccatagatcg cactcgaagc
     1141 ttctgcaatc tctgaagcat ctcccgcagc cacagccata atgccatagc gtcttgcttg
     1201 ttccagaaat tcattgcagg gcgcaaggcc caccgcaatg agaacagcat ctacttcata
     1261 gcctcttgct gttcccggga taggctgaaa tctctcatcg actgcggata cataagctct
     1321 ctggaccttt tgacagccct ctgctcttgt tatcgtggta ttcagatgaa ttggcactcc
     1381 caaacgtctt atcttgtctg catgtacctg atacccattg accctaccag ctgcttcaaa
     1441 aataccagtg acttcgatgt ccgcttggag ggcatgatag gcagcgatta agccaacatt
     1501 acctgaacct acaatgagga tcttgctata tgctttgaca agatcacgat ttacaagcgt
     1561 ctggaaagct cctgccgcta ttactccagg aagatcccag ccaggaaaaa ccagtgcttt
     1621 ctctcttgct ccggcagcaa cgacgagagc actgaattcg acaagcagat aggattcgta
     1681 attgagataa atcccagctt ttcggtcttt gtagataccc acgactggcg cattcgtcag
     1741 tatgactacc gaagggtatt tcagtgcttc ctctttcagg atctcagcaa tatcgatgcc
     1801 gcgtgttcct gcatagcaat cggcttctga tccaaagaat ttatgagtct gcaaaactag
     1861 tttgcctccg gcttgctgct tgtcatcggc cactagaacc tgaaagccca tattacctag
     1921 ctcagcagct gcagcaagcc cagcaggtcc agcgcctatg ataagaatat cagtgcgaat
     1981 agatctgcgt tccgcttgaa caagcggcct atcctgagaa ggcaacctcg gcagtccatg
     2041 aattgtgcgt acatccattc cttgggccaa aggagttacg catgacttct ttggaatgcc
     2101 gtcgatcaac atcgtgcatt gtgagcattg accatttgcg cagaatagtc cctgaggaaa
     2161 gtcatcccga aagtgatagg aaaatacatg cctatccgct gatattaaag cagaagatac
     2221 tggttctccc tcataaccag ttatttcctc tccatcgaaa gagaatttca ccagagtcag
     2281 attctttttt gcagtgagta ttggatggtg ttctattcgg ttcatatttc attcaagcct
     2341 atgattgaat tccaaagctg tcaataaaca tactgctact agaaagcaga ctatcgatga
     2401 tattctgctt atgctatatt aaaaaaagtc aaatgacagc aacaccataa attaaatcaa
     2461 aacgaaagag gaattattgc ccaaatggat gaatttcgcg gcaaatatat caccataatg
     2521 ggacttggcc ttcatggtgg cggaatcgcg agcgcccgat tctttgcaaa agctggagct
     2581 gaagtcactg tcacagatct tcgcgatgaa aaaactcttt ccccagctat acatgaactt
     2641 cagggccttc cgatccgata tgttctaggt cgtcatgaaa tagaagattt tgcccatgca
     2701 gacttggtaa taaaaaaccc agctgttcgc cgtgattcgc cctatctcgc atatgcaaag
     2761 agaatagaaa ccgatatttc tatatttctg cgctattcta aatcgcctct cgtcgcggtc
     2821 acgggatcaa aaggaaaatc gaccatagca tccgccattt ggcatgttct cttccagaac
     2881 aataaaaaga ccctactggg aggaaacatc actgtctcgc ctctcgattt cctagacaag
     2941 acagacgcac aagtccctgt agttcttgag ctttcaagct ggcaacttgg agacttgcga
     3001 ggcacaggtt tattaaaacc atctgtagcc gtgctgagcg atattctgcc cgatcatctt
     3061 aactattatg attccatgga cgcttatgtc tccgacaaac gtgtaatcta tgaatcacag
     3121 gatgagaatt gctggacagt atgtaatgca gatcaaaaat ggggacatgc ttttgcagaa
     3181 gaaactctgg cgcaagtcct ctggtacagt gaaagacctc tcgaggattc agtaggaaaa
     3241 aacgctttgg gtggttggct cacgaaaagc aagaccaaat caaattattc cgatgattct
     3301 ggtccttctt actggtcttt agatcttctc gaaggctatg gcagattcag cctcgatggg
     3361 caagaagagc tgctggtgcc aagccaggtt ttagtacctg gtcgccatca aaaaaagaat
     3421 attctggcag ctgctgttgc agcacgggcc tttggtcttt ctccggaagg tatcatgtcc
     3481 gcactcgcaa gctttcctgg agtgccacat aggcttgaat tcgtaggctc cattcgaggc
     3541 ataaaatggt ataacgacag cacggcaact attcctgatg cgacaattgc agccattgat
     3601 agcttttcga gccccattgt tctaatagcc ggcggaagcg ataaaatgag cgatttttcg
     3661 gcttttgccc aaaaagcaaa gaacctaaaa gcagtaattc ttctagcggg gagtggaaca
     3721 gatcgtctta ttccaatcct cgatgtgaat aagatagcat acaatggacc atttttttcg
     3781 atgcatgaag cagttcaagc tgcagacaag caggcggatc ctggcgatat tgtaattctt
     3841 tccccgggct gtgcgagctt tggtatgttt ctgcatgagt tcgcaagagg tgatgctttc
     3901 aaaagagaag ccctaatgct ttttcgccaa tatgcagaac agggctctac ggcacaaaag
     3961 cgatagcggc atatccaaca aatgatcgat caggatggat gtcgatactg agcttatgcc
     4021 caattagcag gctatgcctg gctcctgcat tgagagcgaa agagatggct gcagcaacag
     4081 ctcctgagga gcaagcagaa aaattattcc tggcatgttt caataccttg tccgcatcca
     4141 ttgcaagcgc tgcgcttatg aaacccttat cgttttcatt tcgaacccat tgaacagctg
     4201 gctctcctag acctctcggc ataaaaccat aattagggcc atagtgggtt aggtcggtgg
     4261 aagcaatggc aacgagttgc tttttgcagg aagatgctgc cctgaagagc gcagatccga
     4321 gttctctggc cttgtaatcc ggcggaacgc gaagccatag aattcgagca tttgggaatc
     4381 gaaacccggc aagtggcaac aatatttcga cggagttatc tatgtctcga tctggagcca
     4441 tttcttggat cccaacgttc ttcaattcta cctttaggga atcaagaact tcaatatcgt
     4501 tctctgcatc tctaacagta cagtcaaaac catcttcatc tccatatagg atgggctctc
     4561 cgttacctaa atgacctccg agaattgcca ctgtatcgca atctctcaag gcccagactg
     4621 ccctggcagc caaatccgca gaaaaatacc atccagcatg tggcgcaacg acggcatagg
     4681 ccgatagatt attctgagat ttggtccagt cagcaacaag agaactgatt tccgctgaat
     4741 cttcaggata ccaacccgaa ggcaggtatc tttttctaat cctgcgattt gtagtcatgg
     4801 cttctatcct cctagcggca atcccatgca ttttatgcat ttgaatcaat aaacaatact
     4861 ctccgactgg gcaggttttt gccgataatg atattatata ctgcagggat ggaaaaatga
     4921 aacgaacatt gcgtataatc ttgttcattg ggacttttgt gcttttagct ctcattgtag
     4981 gatatgtcgc gtggaagatc ggtgagatgc gccttctggg agaacagagc gcatcagctg
     5041 aatattccat cctgcgaaat gcagcatctc tcataacaag tgaagaagat cttggcgatc
     5101 aattcgtaag agataggctc aaatcactat ataacgcaag cacacacctc ctggccgttc
     5161 aagttcttga ccgaaacggg cttgttcttt ggaaaatgcc cgatgagtcc ccctacttcg
     5221 cttcgccagc cacaaatcct ggtagcgtat ttcgtgctcc aggtatgtcg acaataatct
     5281 acatgacacc acttcccgac ggcatgaaac tgatggcact ctattcaatc ttatctcaaa
     5341 gagatatttc gaatattctc cttgttccag ctatcatact tgctgcatgg attgtgcttc
     5401 ttgtgatatt gcaatttgcg ttaaaggaga gacctataga ggcttcagag ataatcggcg
     5461 cagaaaaaat gcccgaaact cccgaaaagc cgcagtatca cgaggagcca ttagagagtg
     5521 ttccctattc tgaagaagct cttcttacta tttctgatga agcctcttct atagcagagg
     5581 aaagcataga aaaccatgcg tctctggaaa atatagaaaa catagaagaa aacatagaag
     5641 aagaagcaga ggaaatcact aataagaaag tggaaattcc ggtatttcca caagctcctc
     5701 aacctgaacc cgaaacgatt ccgatgtcta tctcatcatc tgatgatttc ctgcctggag
     5761 aatttgtcgc tcctgcgcaa cagatcgaag aggaagcaga agaagcaccg caacctgaga
     5821 tttttccatc cacactttca ctcgctgaat tggaacgcag ccttgatata gagcttcgac
     5881 gcgagaaaga gacgtctctg ctccttgttc actgtctgct ttctggtgag ctggatccaa
     5941 gtgctttagc cttgggagtt acaattcgcg actattttgc atcggattcg cttgtcttcg
     6001 agcttgggaa aggctgttat gcggtggttc tgcctggcat ggatgcgggt tctgcgctca
     6061 aggttgcggt ggatctcgac gacgtgctca caactaccgc aagtctctat aaagatctat
     6121 cggaagaacc acccttttat tttggattaa gcgcccgata caatcgcacg atctcccctg
     6181 atcgcctata tagagaggct tttgccgcgc tcaaggaagc acaggagaga agcggttcga
     6241 gaatcctggc atttaagcct gtcagctctg tagcatagag cagtgatata cgctaatcga
     6301 caaactcgat aggaaagcgc atcctatcct ttgttagcac ggtatcagga aatacctcgc
     6361 gcgcctcatc gagcaatatt ttgagctctc gatcggcata tcgcggacta tagtgaatta
     6421 agcccagctt ctttatgtta cctgcatctc gagcaatcag agcggcttga cgtgcggtca
     6481 tatgccgctt gtcgactgcg ctttcgataa gcgcgtgctc gaacatccct tcgcatataa
     6541 gaagatccga atttgatacc tcttgagcaa tttctgggaa ataaagcgta tctgtaacga
     6601 agctaaattt tctaccgctg cgaggctctc ccataacttc cgaaggttct atagagcttc
     6661 tttccgtgag tcgcaccgtc ttgccagatt gaagctcaga ccaaagagga ccacgtggga
     6721 cacctaaggc aagagctttt tcagggtaaa aaaccccggg tcgctttgat tcttccagtg
     6781 cataaccaaa gcacattttc gtgtgcttta gtggaaaggc tcgtattaaa taatcatcgc
     6841 tctgccatat aattccagct tcatatatct ctcttacgat aatgtcataa ttgatataca
     6901 tatccaggag atgacgattc tgatctacaa attccgcaat tcttggaggc ccaaaaatat
     6961 agagcggttc ttccctatcg acctggctgg aaagcattag aatgcctggc agaccggtaa
     7021 catggtcagc atgcatatgg gagatgaaaa ttgcggtgat ctttttccag cgtagattta
     7081 gccttcgaat cgaaacctgg gttccttctc ccgcatcaaa gagaaatagc tcaccctcgc
     7141 gacgtataag catgcttgtt agctgtctat aaggcagggg catcatgcca ccacatccaa
     7201 gaacaaatgc ttctaaattc atagcgaaat gactatagcg gaaatgctat agaatgtcat
     7261 catgaatgtt ccgaatcttt tgaatattgc tttttcgcta tacctaagtc atcaaaatca
     7321 agcgctccaa tgacttgtct cacctcaagg tgagtattct ccatgtatac atcttcagtt
     7381 cggtagatag ctgagtaagc atatataaat ttccatcatc accaactacc atatctataa
     7441 gggaagcatc ggcatcaggt acttgaaagt ccaatttgcc atttattttc ccctgttgat
     7501 tgaagcggaa aacctctcca gataatcctt tatctctcca acgaacacaa aaaatgccat
     7561 tcgattgggc agcgatcatc tgctgatcga tccccaagtc ttcaagactg ctctgcaatg
     7621 gaaaagaatt tagaagcgct ccgtttgatg catctgtctc aatgatccaa ccaccaacag
     7681 gctcgataga aatcgtcact ccagatgaag gatcgcttat ctcgtaatag tagttgatct
     7741 tgagcattac cactcttctt ccattcaaga agcaaggcag aaatgattca agcgaagcaa
     7801 tgatacttga accaccagta ttcggatgat tctcagctat tgaaacaggg attggcatgt
     7861 gttgcccatc ccattttagc acatgcaaaa aggtgccatt tcgatcatag aaatagactg
     7921 ccttccccga ctcggaagag caggaaactg caatatcatc attgctaaga acatctagcg
     7981 aagtaattac cgggaatcta attcctccga tgccctcttg tccaagaaaa gggagctctc
     8041 taccgtcgga atcaaatctc cttacaattt cggcatcaga aacatagagc atttgtcttg
     8101 aatcgctagc aatatgacca ggatcttcaa atgaaacctg aatggcaaaa cgacccaggt
     8161 cttttcctga agaaaagtca ctcatctgga catcctcaag gacaaggggt gcacttcctc
     8221 tttttggatc ataccacatt gcaagggtct ggccataaga agaaatacga agtatttttg
     8281 cttcctcttg gctcagtaga tgaaatattc ctttcctgag gcaaatatcg actgagttct
     8341 tattctgtac agaaatatcg atcgaagaat cggatttacc atagccaagg tcgaaaagtt
     8401 gctgtatctt tccttcttcc atagaggatt ggcagctcac aagaatatta gcaatgatta
     8461 tcaagcatag cagacctaca aatctcttca ttgcgagaat tataccattc cttgaccctt
     8521 tttgagaaca aaagtattct taacacatgt ctagaaacac atttctttca gccctcctgg
     8581 gctcgaaaga agagaaagat atcaaagctg ttttgccact tctccataag ataaacgaac
     8641 tcgagccctg ggctctgtct cttgctgata gcgactttcc tgccatgaca gagaaattca
     8701 aaggccgcct cgccaatgga gagaccctcg atgatctttt gcctgaggct ttcgctcttg
     8761 tgagggaagc ggcacgaaga aaactgggtg agcgtccatt cgatgttcag ataatcggag
     8821 gaattgctct gcatcaggga aaaatcgtag agatgaagac aggagaaggc aagactctgt
     8881 cttcggtaac cgctgcttac ttaaatgcac taactggaca aggtgtccac atcatcacgg
     8941 taaacgacta tttagccgag cgcgatagtc aatggatggg gcaaatctat aagtttcttg
     9001 gacttactgt tggatgcatc ctctcggcca tggacaatat agcaagaaag aaagcctatc
     9061 tcagcgatat cacctatgga actaacaacg aattcggttt cgattatctg cgcgataata
     9121 tggtttggtc tttggatcaa aaggtccaga gagggcatgc ttactgcatc gtcgatgaaa
     9181 tcgattctat ccttatcgat gaggcgagga caccgcttat aatctcaggc cctgcggacg
     9241 acgacactta caaggtaaat gaggtcaatc gcctggcttc gtcccttttc gaagtcaaaa
     9301 agaatcctga gacaggcgat tatcccgacg aagccagggg cgaacttctt gaaggtgatt
     9361 ttaaaataga cgaaaaatcc aaacgagtca tgtttactag cgaaggattg aaccacatcg
     9421 agtcattgct acagaagaga ggattgataa agggaagtct ctttgaccca gaaaacttcc
     9481 aatttgtcca ttattttacg caagcggttc gagctcagca tctcttctct aaagatgtag
     9541 attacgttgt acaggacaat caagttcaaa tcgtcgatga gtttacaggt cgaattcttc
     9601 atggcagacg ctactcagat ggtcttcatg aggctatcga ggcaaaggaa agaatcaaga
     9661 ttgccaggcg caatcgaacc cttgctacaa taacctttca gaattacttt aggttatata
     9721 aaaaactagc cggaatgact ggaaccgctg aaacagaggc cgaagagttt aataaaatat
     9781 acaacctcga cgtaatgatc attcccacaa ataggcctgt tgttcgaaaa gatgagaacg
     9841 accttgtata tctcaatgaa gcagaaaagt atgatgccat aatcggcgcg ataagggaaa
     9901 tacacacaaa gggacaacct attctagtag gcacggtttc catagaaaaa tccgaaaagc
     9961 tctctgcttt gctcattaaa agtggcgtaa aacatgaaat attgaacgcc aagaaccatg
    10021 cacgcgaagc tctgataatt gcagaagcgg gttcgaaggg agcggttacc attgcgacaa
    10081 atatggcagg acgcggaaca gacatcaagc tcggaggaaa tccggagttt cgtgcaaggc
    10141 gcaaggccgg taccgaagca cctgaagaaa aatacagaga gatcttatct cgcgaatacg
    10201 agacatggac taaagattat gaagaagtca agagccttgg gggtctttgc gtcataggaa
    10261 ccgagcgtca tgaagcaagg cgcatcgaca atcagctgcg aggccgaagt gggcgtcagg
    10321 gagatcctgg acggtcggca ttttttattt cgctagatga tgatctgatg cgcctcttcg
    10381 gaggagacaa tttcaaacaa ctcatgggca aggctggcat gaagccagga gagcctatct
    10441 atcatcctct gctcagcaaa tcgatcgaaa gcgcacagaa aaaggtcgaa gaaaggaact
    10501 ttgagatacg aaaacacctc cttgaatacg atgatgttct aaataagcag cgcaacttca
    10561 tttatgaaca aagaaattca atcctagccg atgacaatct tatagagagg gtgcgggatg
    10621 cagcctacga aatgctggag gaacagaatg ctattctcag ccaggcattc aaagataatg
    10681 caaataaggc aatccaagaa ttcggctcgt ggctctttga tagctttggc atagaaatga
    10741 caacaaaaga actaggtgcg ctgtacaaat caggtgggct tgtttctgag atcaagaata
    10801 atcttgatct tgatctcgat gctaaaatac aagtggcagg tgcggagaac ctcaatattt
    10861 tcattagatt ttcttatttg caggaaatcg atgaaaaatg gcttaatcac ctggaatcga
    10921 tggaagctct tagagaagct gtgtatttgc gaagctatgc tcaaagaaat cctttgctcg
    10981 aatataaaat cgaaggatca gatattttcg aatccttgat cttttctatt cggcataata
    11041 tcgcctcaaa ggtctttaga gtaagaatca aagaaagtac cccaacggtc gaagcaacaa
    11101 gtccacgatc tacatcgagg atagaagcgg cacagacaag tcatgaagca gtaagttcct
    11161 ttgtcgacca aagcaaatca gtgcagaaag agtctgcgat agctttggca tccactccag
    11221 ctacagccac ggtggtcaga caaggaaaga aaatcggccg taatgatcca tgcccgtgcg
    11281 gttcgggcaa gaaatacaag tattgtcatg gccgatagaa ccgcttgata tcggaaatga
    11341 tgatattgga cgcttcctag aatggctgga gtttttctat gtcactaaac tgcgaagaaa
    11401 tagatctcat tctaaacgaa gctccacttg tcggaatgaa gattcaaaat gtctatcaac
    11461 ctacttttga ttctctcgta ttagaactat ttggcaaggg aatattaacc cagtattatt
    11521 ttagcatcgc ccataatgcc tgcagactcc atcctttaag tacccctgct cccaaaaacg
    11581 aacgaccctt gcgctttatg gaatgcctac gctcaagaat cagaggaggc actatattac
    11641 acgcagcaca gattagcaag gatcgcatag taaaaataga tattatcaga gcaaacgaaa
    11701 aaggggttgc ggaacaatcc tatttatatg caagattatg gagcggagcg ggaaatatca
    11761 tccttgtctc tgttgaaggt atcataattg acgccctgcg acgcctaccg gctcgcaatg
    11821 aagtatccgg ggcagcattt gtgttgcctc agcagcttga ttccaataaa caaccttcca
    11881 agcattacgt ggttcgcgat ctggaaggca aaggcacatt ttggcaaaaa atagaacaat
    11941 tttatgctca aaatgcggat aatctctcta gatctactct gctcactcaa gtgcacgaca
    12001 gatatgagag aaaaaagacc tcaatagaga ctaaattgaa tcaccttgtt tccaaacaac
    12061 aagaatatga acaagccgag cgattccgcc aactgggtga tattctgctg gctggatatt
    12121 gccctgagat acaaggaaga aaagcatttg cgcgtgtcta cgattattac gagaataagg
    12181 agattcttat cgagatagat caaaatctca atcctgctaa gaatgctgcc atatattacg
    12241 aaaaataccg aaaagcgaaa tctggcctcg aagagattcg ttccgaaatt caatattggg
    12301 aaaaaagcct taatcatttg gacgcttggc tcaatagact tgagaaagag actaatcctt
    12361 tgataatagc caagattcta cgaaaaggtg gagctgctcg ctcaaaagag aagcctgctt
    12421 ttccttgtca atatctagat tttaggggtt ggagaatctt gatcggtcgt tctggcaaag
    12481 aaaacgaaga tatcctgcgt catattgtac gaggttcaga tttatggctg catgcacggg
    12541 attatgctgg ctcctatgta ttcatcaaag ccaagaaaaa caaatcgatt cccccagaag
    12601 tacttctagc tgcagcaaag ctcgcaatat actactcaaa agcccgcaaa aatggtggtg
    12661 gcgatgttca tacggccttt gtcaagaatc tgcggaaggc caaagagggg cctataggcc
    12721 gggttattcc ctatttcgag aaaaatttat atattacttt cacggaagct gagataaagg
    12781 agatttttca agaatatcaa tctaaagagg agaactatgt atgaagcgat ctaagatttc
    12841 tctagttgcc acattgttgc tggcggttag tgtttttatt ttatatgcat cgcctaaaga
    12901 taagattgcg gtgtcggtaa ttagaggtcc ttctgggtta agcagcacat ggatgatgtc
    12961 tgagcttgcc aggaccagcc cgagcgaatt cgaattcata tcggttgcgg gcgctgacat
    13021 ggtagtcgca aaacttctca atggagaaat agatgctggc gttttgcctg taaatgtggc
    13081 cgctaagcta tacaatacag gtgcgcccat aagagctttg gcggtagtgg gcaatggtat
    13141 ggtaaaattc ttgacaacag acaccaaggt gcagagtctt gcagactgca aaggcaagac
    13201 aatctacatt gcaggacaaa aagcgacccc tgattatatc tttcaattcc tatgcgcgca
    13261 aaaaggcctt caaatagaaa gagattacac agcaatctat agtctggcat atcctgagat
    13321 agcagcaggg cttgcaggcg gaaagatatc ttatgctgtg ttgccagaac cttttgcatc
    13381 tcaagcgatt ctcaaaaatc ctgctataag aacgcccata gacatcacaa aggaatggca
    13441 ggcagcgaca gatcagcctg attacccaat gtctctattt gtcgcgcgaa aagaaatgat
    13501 tgaagcatat ccagataagg cgagaatctt gcttgattcg tatcgcgcct ctatcaataa
    13561 ggctattgag gatccgcaat tgatagcaaa acttgcagag aatctcgatc tgggcgtcaa
    13621 tgctcaaaca gcaattatcg ccataccaaa atccaatttt gtatttgttg aggcttatca
    13681 ggcaatccca tccatcgaag ctctgctctc ggtatttttg aaattcgatc ctgcaagcgt
    13741 tggtggtgtg ttgccaagca aatcattcta tgcgcaaatc aagtgaacac cgttttatgc
    13801 tgcttcgatg gcagctatta gggattattt catttatctt attatgggag ataggagctc
    13861 tccttgtaaa aagcgatcta attctgccaa gccctgctcg agttgccaaa gctctttatc
    13921 agctttttgg aaagaatatt ttttggcttt caattggagg ctctttcctt agggttttcg
    13981 aagcgttcat tcttagcggc cttataggcc ttgctactgg cactataagc ggcattaagc
    14041 ctcaggcgcg ggctttcttg aatccattta ttgtgggaat tcgggcaaca ccagtcatgg
    14101 cgctgatatt agtggctatg ttttggtttc cggcctggca ggtacctgtt ttcagtgcaa
    14161 ttctaatggc gtttcctgta atgcatactt caacggaagc aggcgtgcat gcggtagata
    14221 agaagttact tgagatgtcg acctttttta atgttccttc atcatttatt ttctggcggc
    14281 ttcgtttgcc atccgcactt ccatatatcc tatctggagc aaaaaatgcc ctggggctct
    14341 catggaaagt ggtagttgct ggcgaggtct tgagtcaacc gcgcatggcc ttgggtaccg
    14401 gtatgcagga ggccaggctc tccattgaaa cagccacggt ttttgcttgg gcaataatca
    14461 cgatctttct ttgcggtcta agcgagtatc tattcggact tctcgcagat agattaagcg
    14521 cgcgatttca acaatcttct caagaagaag accaggatct gagctgaaaa tgaatatcaa
    14581 gatagataat ctgagaaaaa gctatggagc tctcaatgtg ctcgaggatg taactttttc
    14641 tctcgaagaa tctcgtataa cctcaatttt agggcccagc ggctgtggca agacgactat
    14701 cctcaatata atgtctggaa tcttacaagc cgatagcggt tctataattg gcattgaggg
    14761 ccgcaaattt tcctattgtt ttcaagagcc gagacttctc ggttggctca gcgcagaaga
    14821 taacctgcga tttgccttga gctctctatt tcaatcctct cctagaacaa acgacatcga
    14881 attgagaatc gaacgctttt tacgagaagc tggactttgg gaattcagga aatttaagcc
    14941 tagccagtta tcagggggaa tgcaaaagag gcttgctttg gcaagggcat ttgcttttcc
    15001 agccgatatg cttcttctgg atgaggcctt ttcagaagtc gacttaagaa aaaagatgga
    15061 gcttatggag gattttcttc atttatggaa ggatgaagag ccgactgtcg taattgtcac
    15121 acatgacatc cacgatgccc tatatctagc tgaccaggtg atagtcttga gtcagcgccc
    15181 agccatagta gaggggacta tattgattga tatacctcat gaaaaaagga cttttacttc
    15241 ttcagaactc tatggatatg aaaaggaact atatcgcctc ttaggcctcg atcgaactgt
    15301 ttagagcatt attgcaatac tctagaagag aacgggcatc aaatggcttg tgcagcaaag
    15361 cggctcctgc aaattgctca ttttgataag agtcataccc cgtcatgaag acacatgctc
    15421 tggcctgacc attgttcttc atcctatccc ataattcgtt tccatatacg cccggcagta
    15481 ctatatctgt gaggagaagg tcgaagttcg ttctctcagc ttgaagaata gcctctccgg
    15541 cattgataca agcaccaaca atagcacctg cactctcaag gattcttgtg cacgcctcca
    15601 atattgactc atcatcgtca acaacaagaa tatgctttcc tttcaatatg ttattaggtg
    15661 gattgattat ggagagcaaa gagtattctt cctgggctgt gagcttggtc gtggttttag
    15721 tctctaaaag aattgggaac cagattttga ttgtggttcc tcttcccttt tcgctttcaa
    15781 gagtaatgta gccctcattc atttctacaa gagatttaac gatagataat cccaaacctg
    15841 ttccttgatt ttgcttttta gtagtaaaaa aaggctcaaa tacccttttt atattctcat
    15901 tatcaatgcc agctccgttg tctttaacta ctattagagc atagtcggct ggctgcatca
    15961 tgatttcttt tggataaagt gcgttttgcc caaattggct aagctggatc gatatatgag
    16021 gattctcggt cttttccagc gcatccctgg cattcacaac aagatttaaa agtatctgtt
    16081 ccagatgcga tatgtccaca ttgacaacta gggactcttt gggaaggcta agtgtaaggc
    16141 ttatgttctc agggataagc cttttgagta tcttataggt gtcgcccatg acctctttca
    16201 gatctattgg agcaggtgaa taaggtttgt gcctagaaaa gcccaatagc tgtcgcgtaa
    16261 gtccagaagc cctattaaca actctctcta ttccctggat atcttcattt atttgagaat
    16321 catctggaat ttcaagcttt atcatttcca aaaagcccat tatagatgtc aagaaattat
    16381 ttagatcatg agcaacacca cttgtgagtc gtccaatttc ctctctcttt tgtgactgaa
    16441 acagctcctc cctcaacctc ctagattccg tgacatccaa gaattcgcat aggatttgtg
    16501 cgtcgctacc ctcctcgcga aatctggaaa agctgcagat aagatcgagc gatctctgtt
    16561 cgaagctatc ggaaatccta tttgtttgaa tctctctctg tagaatctgg ttttcttcaa
    16621 ggctttctat tatctcgctc cagattatat cattgggcaa gatatccctg acattttcca
    16681 ctttcatcgg attggaaaag ctcgattcaa atactctgtt tctctcaagg attcttcctt
    16741 cgatatcggt gacgcatctt gctgataggc tataagcaaa taaatccttg taacggcgct
    16801 cactgtctct cagctttttt tccatcgatg aatggtatag agccgtctcc acattaacga
    16861 taagttctcg tgtagaaaat ggttttacaa gaatgccaag aggctgcgct tcacgtagtc
    16921 ttgcgggaat acttgtaacg ccatgatcac ttattaccag gcaatataga ccggtctgtc
    16981 tatgtatcgc tactgcagcg tctattatct caggttgatt gtccccaata tcgatgataa
    17041 aaagttcatg ctcgagatta ttgagtttct gatatgcctg ctcgagttct tcatagatgt
    17101 ctacctctgc cagcccttct tgttcaatgc tctttgagat gtctagcgct attaaaatat
    17161 cggattcaag aatggcaact cttggcatgt tgcgggcctc cttgcctcca tcctgcttta
    17221 gtcgattgtc tactgaaggg actttcaaat aattgtagca ctttcggtgg aaaactccaa
    17281 atggatagtg cgttcgaaat atccactaag tctagacttg gccgaaaatt caaggataaa
    17341 tactttattt ttttcatcga gccgaaattt gtaaacggtg atgacgtcgt tttcactaag
    17401 gcctatttca tcggctggct cagcgggaca tacccattgt atagaataga gcccccctac
    17461 tttagtcgat tcttttaatt gagtaagtct agcgccgaac agtccctcaa gaatcgaatt
    17521 cttgtgaatt ctgtcccatg caggtatcaa agaggctcca tctgtatggc ttatctgcct
    17581 caatctcctg actggcttaa agtcgcgctg cgcttctaaa aaacataatg cacctttcgg
    17641 aacagaagcg agttgaaatt gaattgaagc tatgtccttt gtgagtttcc catttataga
    17701 agtaagtgta tcccctgcta tgaatgccga aagagatgga tcaagatcag cgagaaccag
    17761 tggactctga ccactgcctt ttcccatcat taagcctaac cttgcatgaa tgacttcctt
    17821 tctctcgaat agatcaggaa gagacaatga aatccattga acgggcagtg caaagtttat
    17881 gttctgaaat gatggcaatc cagcaaagac catgccgacc aaaactccgt tctcagaaaa
    17941 aagcgggctg cccgagttgc cctgattgac cggaacatcg atctgagcag cctcgccaaa
    18001 agatattatc tttctgctca atgagctaac aacaccagcc gtaatgctgt tttcaagacc
    18061 gatcggcgag ccaacggcaa agacacgatc tcccgcatga aatctcattc ccggaggagg
    18121 cagcatgact gtgctgcgag catgttctgc tgactgtatc agcgctagat ccaattcttc
    18181 gtcccagcca aggacttttg aaggcaccct aatctgagga ttatcggaag gtcggatact
    18241 gatacgcgaa tatcctttat aggttggatc aatctcgctc tgaacaacat gatagttagt
    18301 caggtataat ccctcagaaa tctggaaagc cgtgccaagc acgcgatctg gaacgctgta
    18361 tccgccttcg accttgaacc cccgatccac atatactgtg accacgctcg acaccgcatc
    18421 ggctaaccaa tcttggtcgg aaacaaggat agatggagag taactcatct ggccagacga
    18481 gagcgattca aaccaggctt ccgcattgac atcattgttc tccttagctc tctcagccca
    18541 agtaatgaaa tattcttttg gatctcttgt ctcgataaga ctcttcaagt tttctataga
    18601 aaaatcctca atcttattga aagaatattc ttcctgctct cctaagtgag cctttgatag
    18661 cgttgctttg cctatgacat aaccatgttc tttgaactta ttctcagctt cccagagagc
    18721 gagctgcaat ctcatcgctg gcgatgctat catcgagagc tcttcagata caatttcacc
    18781 aagagaatca taaaaagata catattgaac caaagcatca gcagaaaaag agagccgacg
    18841 agccgtgaga atatcctgac ttgcgatggc ttttttaaag agaagaccga tattttctac
    18901 ggcttttcta aaaagcacaa ttcctttctc accgatgatg ctattgtcaa aagattggtt
    18961 tttctctcgt tgctgttcga atagattgga aatcacagcg atgcactgtt caggatcggt
    19021 atctacaagc agatctagat ggctaataat tgaatctcca gtagaattgg catttggctc
    19081 attctcaggc aaagatgaac agttgcttag gattgctgct atactcaagg acaaaaaaag
    19141 aaccagaata gcagcttgcc gtgtcttcat tcaattcctc aacatggcgc gatcccctct
    19201 tccaggagaa ttttgccctc aggcaggcca agaagctctc tcacttcttt atcttccagc
    19261 gtttcaacct gcataagctt atctgcaagt ctgactagtc gattcttttc ccgctttaga
    19321 atattaattg ccttgtctaa agcagaatca agaatgcctt gtatcgccgc atctatcctc
    19381 tgtgccgtcg cctctgaata atccttatgt tgtgctattt cccgtccgag gaaaataggc
    19441 tcttcttcct ctccgagaga gattgggccg atttcttcag ccattcccca ttcgcgtacc
    19501 atcttttggg cgatctctgt agcttgctta atatcctgtg cagcccctgt agtggtctcg
    19561 ccatatacaa tcttctctgc ggcatatcca ccataagaaa tcacgatacg atcgtaaagc
    19621 catccatagg ttctggagta tgtatcctta tctggcaaag ataaagccaa gcctagagct
    19681 cttcccctgg gaactatagt caccttatga agcgaatctg cgtgtgcaag aaaataatgc
    19741 aggagtgcat gtcccgattc atgaattgca gtggctcgtt tttcttcctc agcaatcacc
    19801 aagctcttgc gcgctacacc catcaaaatc ttgtctcgtg catcttcgaa atcgtccatc
    19861 tcgaccagct ctttgttttt gcggatcgca aagagagcag cttcattgac tagattagca
    19921 agatcggcac ccgaggttcc tggggtcgcg cgagagagtt tctccacatc tacatcacca
    19981 gaagttggaa tctggcgcat gtgtattttg agaatctcgg ttcgctcatt tacatctggc
    20041 attgcaacaa caacctgtct atcgaagcga cccggtctca ataatgcggg atccagtaca
    20101 tcaggcctat tggtagctgc cagaacaatg actccatcct ttgtatcaaa tccgtccatc
    20161 tcaacaagca tttgatttaa ggtctgttct ctttcatcat gacctccccc aagtccagat
    20221 ccccgggtcc tgcccactgc atcgagctcg tcgatgaaga gtatgcatgg agcatgtttc
    20281 ctgccttgtt cgaataagtc acgaacccga ctggcaccaa caccgacgaa catttcgacg
    20341 aaatccgaac cagacatatg aaaaaagggg acatttgctt caccggctgt agcctttgcg
    20401 atgagtgtct ttcctgttcc tggcataccc accagaagga cccctttggg tatcttagcg
    20461 cccattttga ggaacttgtt cggattcttg agaaaatcga cgacctccat aagctcatac
    20521 ttggcttcct tttgcccagc gacatcctca aaggtgatct ttttattgga attgctatac
    20581 attttggctc ggctttttcc aaaactaaat gccttattat tgccctgcat ttgtctcatc
    20641 attatccata tcatgataaa gccaaatatc catggagtga gcccaaatag tatctgaaaa
    20701 ggacttgtgc cactgattgc tccggttacg cttactcctt tttcttcaag cttagccatc
    20761 agctcagcgt caaaataggg tattcttgtc gtaaaaggca tttctgcccc actcttgccc
    20821 ttcaatacac cttctatgtc cctctgatcc atgattttga tggactttac ttctccgagt
    20881 tcaagataag ataaaaatga agagtaagaa atctctcgcg aactctcgtc ttgcccaaag
    20941 aatagaaaga caactaatag gacaattatt gacaaaaaca ggacaagagc cgctctattg
    21001 cctttgggag gaatcgacag atccggctta ttcccatctc tattatcgtt ttgatctggc
    21061 atttatctaa aaatctcctt tcctcgatag gaaaatatac agtatttttc ttttttcgca
    21121 cagcaaccgt gaagaataat ccgattttat actaaatctg ccatcaaaag tcattgtaag
    21181 aatcgctgca atcccatatt catcctctag aatagccaca atatgactta tatcagcaga
    21241 gattttctgg tttttcgaag tcgagcccaa gtctttcgat ctatctccat cacctatgtt
    21301 ttcaccttcc tttctatttc gaaaaataaa aggaaaagaa agcggtatcg atagacgcaa
    21361 aaactgctcc ttgtctattg aggcattatc taaagaagga ggttgtacgc tttgagatac
    21421 taccatcttc aatggcccgc attcatactg gcctggctct cctaccctca gaaagaactc
    21481 atcaaatctg aataaaccct taagttgcga aatacttcta tccaaaaatc cggaagaaga
    21541 attcgccttt ccaatgtact tgacaactcc attggagaga tcgaattgac atcttgctgc
    21601 ttcgactcta ggtgctcctt gtagaattgc acgttcaagg gattccagtg caccttgact
    21661 gacagcatct tttaatccca aatgcataaa aaacagcctc aaaattctcc tgcgcaccaa
    21721 agcttcttct tttctgaaga gggcttgatc cagtatggca tccttaccca gacctctcat
    21781 cacttttggc atgctctgac aagcaattga ggtgattaga gtgtttaact cctgggcttg
    21841 ccaccgataa gactcgattg aagatctcca agaaggaaat tccttatcga gtagaggaat
    21901 gagcacatgt cttattctat ttctcaaaaa agagagctcc ctatttgttg agtcttctga
    21961 ccattcgagg ttattttctc ttgcaaatgc ttctagctcg tagcgtctaa aactcaaaag
    22021 ggggcgaata acattaatac cttttgtgct atcgactaat ctcgatgctg aaatgccacc
    22081 gagagtctcg agacgtccac cgcgaacaag acgcagcaag aaggtctcag cctgatcatc
    22141 ggcttgatga gcagtgacaa tcgtcgagat accaaatctt tgtgctgttc ttacaagagc
    22201 atggtatcga taatgccttg ccgccgcctc gatgccacag tttttcctat gtgcatattc
    22261 agaaatggcg ccttgtctta tattaacaat ggttatagga atactataag ccttgcatgt
    22321 tttttggaga aatattctct ctctttcgag ctcttcctta gatcgaatat tgtgacatac
    22381 atggatggcc cttatgttat catgatcgaa aatacgaacc aggatataaa gcagaacgct
    22441 tgaatcgagt cctcccgaaa aggcaattgc caatggctta tgaaaatcta catgcatgtc
    22501 gagaagaaat ttcttgactt tgtattcgag ggttttcatt ctatttgcct tgcccgggct
    22561 tgcctctgtg caccctggtt tctgtcttgc agatccagat gtatcaagcc tagcaataaa
    22621 aagcccagct ttccagcatc tgaagccgga acactggact cttaagaata aattgaagta
    22681 ttgaaaagct atttatgtac agcttcactt gtcgtttctg cagattcctg ctctgaagat
    22741 gcaacagctt ctcctgcttc ctcagcttct ggctccaatg ccttttcagg ctctccatgt
    22801 gcaaacttta ttatcgcaaa tacggtgtca gggttagtaa ccaactttac cccttcaggc
    22861 aaggctactt ccttcagatg aagggcatga ttcgcttcaa gatctgtgat atcgacttcg
    22921 atcttttctg gaaggtgatc ggaatcgcac tcaacttcga tctcatgaat tgaatgctcc
    22981 agaatcccac catctttgac accttttgct acgccattca ggtgaagagg aactctaaca
    23041 tggatatttc tatcgtgcct tacaagatta aaatcaatat gtatgacatt accagaaatt
    23101 gtctcctttt ggatttcctt caatagagtg gcaaattttt tcccttccaa atcgaggtcg
    23161 atgatcctac tctcggtgac acgttttgca acattagcaa actccttgcc ttcgagaaaa
    23221 accgagatag gctcaatccc cttgccatag atctgagcgg gcaatctgcc tttttttcgc
    23281 tcttgcctga gctctccttt agtcagtttc cctctcggcg agactctcaa tgcaatatgt
    23341 tccatatttt cctactcctt gagccatatg ctttatcctg ggacggcagg attcgaacct
    23401 gcgcatgccg gaaccaaaat ccggaggct
//
LOCUS       DAOOWY010000021        23322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2211_length_23322_cov_10.749474, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000021 DAOOWY010000000
VERSION     DAOOWY010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 23322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23322
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2211_length_23322_cov_10.749474"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2314)
                     /gene="greA"
                     /locus_tag="PK105_03035"
     CDS             complement(<1..2314)
                     /gene="greA"
                     /locus_tag="PK105_03035"
                     /inference="COORDINATES: protein motif:HMM:NF011309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOU60715.1"
                     /translation="MDQSPLSEKLQLMLNEEKWTRTLMANYSIANLKELDRFIEEITA
                     QNVADDIIKLCIEHLSQTKNSVIALYVAGMLNIMRQPADETYLIQLMDLFAESKKSNI
                     VEYICNRMLDFGENRIALVRLAECYTAENKIEQRYTVWESLVRIDTEEAELAQLIAEW
                     KEKNGLIEEAIDFYKKALIRYINKGLFSNIKELWHKLIQLSPEDYDFFLHAQKKIAKQ
                     VSPEKAATILMDLYDWYKKNEKWDTALNILKLIVEYDERNPALRKEFLECYSKIYSGH
                     SMFDECVRLSTLTQSYRSLHETIPDFEKRIAFDRGNYVFHRTWNIGRISNITNDEVVI
                     DFAKSRNHHMSLKMAFDSLITLSKDHIWVLKATWPRDKLREKVKTDPAWTIKIIIKSF
                     DNRADMKRIKAELVPSILSISEWNSWSTRAKEILKTDPMFGNAQDDISTFIVRDRPLS
                     FEEKIYTQFKAEKNFFSRVQYFRDYLEMSEEVDTDYFEEMLSYFVSFLKLTQVNEFVV
                     GSYLLLKELSGRYPRMQQYVTMRFVDLFEQIEDDIVGIYLGLKDTELKNQFIQNIKLY
                     IQNWPDIYIQLLPYARSAKMIDALESNGHEEKLKRMAMYIVENYKDMREAFIWLAKDL
                     KDKAWMKELGIVREKIILTLLHILDITFKEIDIHKDTTENRKINKLVQNILFKENDLE
                     EFLNEAPQPVAERVYSMLSDIKEVDPVIKLDLKKKIGERFPNIKFYDGNERLTVSRGL
                     MVTANKFDEKKRLLGHIMEVDVPANQKEIAY"
     gene            complement(2328..3284)
                     /locus_tag="PK105_03040"
     CDS             complement(2328..3284)
                     /locus_tag="PK105_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60716.1"
                     /translation="MAHQPRPKEEATIAEENLYLAYESLKNADIQSADSFLENALKAD
                     FEHPEVLFAMKCLHFWKDLISQMPETGSLLERGDFLCGSWKKFILFLSHIGDSFDTAR
                     YVYRRFIFGLALDQYLAIPDEEKEAFGPLLDLRLGRARKATGDIETALIHLERAAQAR
                     RNDANILAELADVYAMSGEARLSKALFREAFFIDAQAIEIDFLESELIFKIIENIQKL
                     GYSGSDIAEWIPVYAEIWGVFNIKRELSGAEYNRISASARQLEIQLRESHQQRSSLIP
                     RLLKKYFWMADYLKAREDTKELHSVLLKIKIIDQSIYESYIG"
     gene            3441..4121
                     /gene="rpe"
                     /locus_tag="PK105_03045"
     CDS             3441..4121
                     /gene="rpe"
                     /locus_tag="PK105_03045"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOU60717.1"
                     /translation="MKEPIIAPSILSADFYDIGSAIRHVEESGCPWLHLDIMDGHFVP
                     PITFGHKMIADIRAKTELFLDAHLMIEEPERHINAYIESGADAITFHLEACVHSHRLI
                     QSISRAGIKAGISIVPSTPVSNIVPLLEFLDLVLVMTVNPGYGGQKLIPFCLQKVEEL
                     RRIREQRGLHFLIAIDGGVNAKSISDIVIQQPDVLVIGSAFFNAQDRKQFVADFRNAW
                     TSPIGNID"
     assembly_gap    4129..4228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4306..5160
                     /locus_tag="PK105_03050"
     CDS             4306..5160
                     /locus_tag="PK105_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU60718.1"
                     /translation="MKKLIMKMFAVIMFILASFSYAQANKTSDEFSYAFDIFRNGDFG
                     RAGAAFADICQTESNMEILADSCFMAALSYFQAADFPTSYEFCEKFMQSFPSHPNMPD
                     MQFLKGRILFKLGLYADAISVFDIFINRYPDSPFCSSVLFWKGESYYQLGDFAQALSI
                     FNKIKEAWPNTEATSLTAWRITVMASESREAKYARLAEFESRRNIYRDVQQMQEDVYG
                     EQRHLRDYLWSKAIRKYSARPNIETRELSADETSRNNRLNMLLEAKERALNLLIWKID
                     NYLKDITP"
     gene            5157..6221
                     /locus_tag="PK105_03055"
     CDS             5157..6221
                     /locus_tag="PK105_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60719.1"
                     /translation="MKKPVCFFILLFIVIGALFAIDIRNGLVRIATDDLTLQPALYRL
                     VNISGKSKYEPLWFSGDPRTSFIVLNIDNRIYRMGASKEYKTSQRQIKNGIEIEYRSV
                     TNRLTQRLLFFTSPFSRVADGFVIEIEIENYTTREMKVMIKEVCDTVLGEKEGFHFAT
                     SLNARVTDEMILASDSDEKYVVSPGQAASLALLLDEDQKPDMVVIANWKRLSDSKFYY
                     DSQLMKGFTLSSFSINDSALGLYWNERIIPPKGSIKVASTWLTGGSGSEFIDWLSLNY
                     PFPRQEPQQVLESPPVVLEEQSIVNAPILDIKAIQDLISEIDRAIDNIENLTDNELQD
                     IMTKLNLLEQSEGLAAPQSN"
     gene            6236..6697
                     /gene="dtd"
                     /locus_tag="PK105_03060"
     CDS             6236..6697
                     /gene="dtd"
                     /locus_tag="PK105_03060"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOU60720.1"
                     /translation="MRAVIQRVSEASVTYRTSREEKFCGSITKGILIYLGIASDDSDD
                     DAEYLADKIAHLRIFMDSNDKMNLSILDLGYEALVISQFTLHADVRKGRRPSYSKAAG
                     SEKALMLFDLFCTKLLENGVHIQTGKFQEIMRIHSINDGPVTILLDSKKAF"
     gene            complement(6788..9574)
                     /gene="ppdK"
                     /locus_tag="PK105_03065"
     CDS             complement(6788..9574)
                     /gene="ppdK"
                     /locus_tag="PK105_03065"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOU60721.1"
                     /translation="MPRRKFVYAFGAGLAEGDGSMKELLGGKGAGLHEMTKIGLPVPA
                     GFTITTEVCELYYKNGKKWPEGLEAEIQKHIKNLEKVTKKTLGDPRDPLLVSVRSGAP
                     VSMPGMMETILNLGLTDASVEGLASKTGNRRFALDAYRRFIMMYSSIAMGIDREIFDK
                     AFEEIKETKTRPRIGIPSEQKVNDTDVNEEELGELIKSFKKIYRDHIGTDFPQDSWEQ
                     LKGAINAVFDSWMAEKAVTYRKVEKLVGIKGTAVNIVQMVFGNKGDSSGTGVCFTRDP
                     NTGENIFYGDYLINAQGEDVVAGIRTPIKLAELEKLDPKVYKQLVDIRRKLERHYRDM
                     QDLEFTVEEGKLYMLQCRTGKRSPIAAFRIAVDLVKEKLITKEEAILRIKASDIEGIF
                     YPMIDKSNATALKKSFLVQGIDAVPGAAVGRVVFNAKVAEEWEEKGEKIILVRKETSP
                     EDVGGMHAAQGILTATGGKTSHAAVVARGWGKCCIVGCEKLEIDYENRHFAVGDKIVK
                     EGDYITLDGNTGNVYLGQLKLVKPEPPKAYKTLMSWVDRTRKLKVRTNADTPYDAEKA
                     RELGAEGIGLCRTEHMFFDSEERILAIREMIIADDTESRKKALAKLLPFQTKDFEGIF
                     RVMDGLPVTIRLIDPPLHEFVPHDEEGQRELADAIGVPIEKVIQRVEQLHELNPMLGH
                     RGCRLTITYPEILDMQVTAIITAACNMTKNGIKVLPEIMIPLVIDDKELKILESRARE
                     IAAAIIAASEVPLKYMIGTMIEVPRAALLADRIAKVAEFFSFGTNDLTQMTLGLSRDD
                     AGRFLPDYIDEKKGAIFKADPFQNIDQDGVGMLIRMGIEKGRSSRPRLKVGICGEHGG
                     DPESVKFCHRVGMNYVSSSPFRVPVSRLAAAQAVIEDAQAAHKSTRKPGRPPKAASSK
                     AKRPRGRPRGSKNK"
     gene            complement(9747..10391)
                     /locus_tag="PK105_03070"
     CDS             complement(9747..10391)
                     /locus_tag="PK105_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60722.1"
                     /translation="MKSLGKILLFMAFTISLTLCFISCIGIEASTKIDSKGAGTFSVV
                     YQISKDFVDFGSEETTPSLPLALSREDIERGLQGSEGISLKSYSKTNNANNTIISFAL
                     NFASPENLINYLDPTRSYTRFSEENGSYSLKFDFGNDIPPLDPDMKKYIFEQFSSYRF
                     ILTIETAKSPPPEVSIKYGDFIRKTSSGKKTILECAMADLLTVSSSPQIEIVWR"
     gene            complement(10405..10683)
                     /locus_tag="PK105_03075"
     CDS             complement(10405..10683)
                     /locus_tag="PK105_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOU60723.1"
                     /translation="MLRLVLKLNETSTIKDKRRVVSGIKDRLQHRYRLSCAETDLLDS
                     LVFAEIGAALVSNSAQYGEKVLQDAVTFIEDHYPVELFEVQMHYEIYG"
     gene            10762..13011
                     /gene="metG"
                     /locus_tag="PK105_03080"
     CDS             10762..13011
                     /gene="metG"
                     /locus_tag="PK105_03080"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOU60724.1"
                     /translation="MKRRLITSALPYVNNVPHLGNLIQVLSADVFARFCRLSGYETLY
                     VCGTDEYGTATETKALEEKVSPRELCDRFYAIHRDIYEWFEISFDTFGRTSTPEHTEI
                     TQSIFLDLDNNGYISSQSIDQLYCDTCGRFLADRYVRGTCPHCGYDGARGDQCEMCGK
                     LLDPMELLNPKCISCGGTPRPRSTRHLYLDLPAILPKLEAWMKDASVKGFWANNAIQM
                     TQAWIRDGLHPRAITRDLKWGIPVPKEGFKDKVFYVWFDAPIGYISMTARLAKERGFD
                     WKSWWQKPDEVELFQFIGKDNIPFHTVIFPSSLLGTGKKWTMLHHMSSTEYLNYEAGK
                     FSKSKGIGVFGNDAMNTGIPADIWRFYVFWNRPETSDHTFTWADFQEKVNGELIGNLG
                     NLVNRTLTFISRYYKGIIPDGTPDEAFWKEVTDREQETSKLLELADLRDAFRTIFSIA
                     DLANKRFQYEEPWKARIDNPKKASSLMRDLCYVLKDLAIMTYPYMPRTAEKLASFLGI
                     EIGHGKLGWADLGLRGDLKRIEKHEVLFNRLEDELIASLRAQYSGSQKEREEMAEQNR
                     KSEGQLPVSEEEDHNDRLDQIIDLCVARIIKIERHPKADRLYIETLDDGSGLERVIVS
                     GLVPFYREDELLGKNIIIVNNLKPAKLRGIESKGMLLAASTKTPEGNDIVEVLTAPKA
                     NPGERVLPEDIDMENPAETVPKESIDIETFLRIPMYAKDGYAWIKGQRLMAHGEPIRL
                     EKVMDGKIG"
     gene            13023..14309
                     /locus_tag="PK105_03085"
     CDS             13023..14309
                     /locus_tag="PK105_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOU60725.1"
                     /translation="MSDVLEQSAMDVLPRIEISPILSAIDCLPECCDQGFLQSKFWGL
                     FKSRTGWQAYSCTYSFYNKDLSGRILVLKRKFAKLFSFLYVPFGAAELSSIPERWITL
                     AALGRALGESFGGRDVFIRFDLPWECDVDPAKTKEEKKPDIENSLGFHISGLYKGTDV
                     QVPDTVILDLRDEEQRILAKMKPKWRYNIRLSQKKGISVADEGFSSLPLFMSLYKETA
                     RRDRIAIHPESYYKTLFETSSEISEVAQENSITTTRPRLSLYVARHKDEVLACIIVLH
                     FGNISTYLYGASSSEKRNLMPTYALQWQAILDAKRFGADKYDFFGIPPDDKDASHPMA
                     GLYLFKTGFGGEIIHRYGAYDISLRPFLYFAFRTAEKMRSFWHKRIKKSLNKKTVGLS
                     GKATKESTIRNVHQGQVSEELASLSSDSENTKPSNP"
     gene            complement(14237..14815)
                     /gene="amrA"
                     /locus_tag="PK105_03090"
     CDS             complement(14237..14815)
                     /gene="amrA"
                     /locus_tag="PK105_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOU60726.1"
                     /translation="MEFAISPEDQKKLLNYARLTIHASLKGEKEIDPPHLSTSLKCGA
                     FVTLKKNGSLRGCIGRIQGDANLPSTIKQMALAAAFEDPRFPPLHEEELPLIEIEITL
                     LSPLYPISPEEIVIGKHGLLIYANGRSGILLPQVPIEFGWDKETFLEQLCWKAGLPSN
                     IWKSPLARLYGFEGFVFSESELKEASSSDTCP"
     gene            complement(14819..15640)
                     /gene="amrB"
                     /locus_tag="PK105_03095"
     CDS             complement(14819..15640)
                     /gene="amrB"
                     /locus_tag="PK105_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOU60727.1"
                     /translation="MSEKKIARSPLLSGIFYPSEPQMLMSEIDGLSLYAKRIPCPAFG
                     IIAPHGSFLYSGKISMESWGAILGSNPSIIVIMGPSHLPYEDGVFLPESTRFDIPGAC
                     LNIDLNLRDYLLNKIPMLKKDDLPHLEEHSIELQLPFASHFFPGVPILPCIISGKNPL
                     TIETATRLFEEISRYIGNYAVFVLSSNLAVSDSPEECDLLSKDFIYSLSRPVAEFTGN
                     VRACYNSFCGEAVIRAFRIAFPEARAELLDYDNSSKHREYCDELVVGYGAIGFKR"
     gene            16113..17261
                     /locus_tag="PK105_03100"
     CDS             16113..17261
                     /locus_tag="PK105_03100"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOU60728.1"
                     /translation="MKDIFIMHHAEYDTAPDCTSAAPTIVKILGEHTDFADGLVLSAS
                     LSFEVRVAISFRKDNSLRFYAADFNERKRANVATFKYKREDRWANHVKSVCDYFWRVF
                     NLDPRGLNVTIQSNVPLGLGLGISSAINMATALDLKTLYDLDLKLEELAFHAWKAQSL
                     FFEKELPLTNYLGITAPAGRSFSIIDLHDKKRRGVQFLPERWEMLLTDSKVPRSSVDA
                     ELEQRIADCKACLSVLVPYHNRSIRDIAPQELDELLGIVPERKRRRCLHVLEEVRRVI
                     EAEESLSRQDYIGFGRIVNKSHASLRSLYEISCPEIDWLTKRALELDDVLCSRLVGQG
                     FGGSTLTILNNEKKQAYRNKLEDYERIFGFKPIIHEVSTGAGLRILEH"
     gene            17300..18061
                     /gene="surE"
                     /locus_tag="PK105_03105"
     CDS             17300..18061
                     /gene="surE"
                     /locus_tag="PK105_03105"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOU60729.1"
                     /translation="MRILLTNDDGIQSDGLFALIDTIAAMKDFNGKSLHQVWVVAPEH
                     ERSGVSHAMTLKKPAKLRRLGDSRYSCSGTPVDCIIIAGLAILHEKPDLVISGINRGP
                     NLGTDIVYSGTCGAARQAALQGIPAIAISCAAHCDNLEYEGLTSFLSANLKELVALWK
                     PDSFININGPSTKSRDLKAVWTVPGKNKYIDNLKCFDGSDGYTYCFLTEGAEERMPDA
                     LSDHHAISQGYIAVGLVEVHPRAFHEPSLNGQFVF"
     gene            18155..18227
                     /locus_tag="PK105_03110"
     tRNA            18155..18227
                     /locus_tag="PK105_03110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18188..18190,aa:Thr,seq:tgt)
     gene            18251..18333
                     /locus_tag="PK105_03115"
     tRNA            18251..18333
                     /locus_tag="PK105_03115"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18285..18287,aa:Tyr,seq:gta)
     gene            18433..18915
                     /locus_tag="PK105_03120"
     CDS             18433..18915
                     /locus_tag="PK105_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOU60730.1"
                     /translation="MNPELPIKDIYFQCMRCNACCSGQPGFVWLSKRDLMALSEYFDL
                     SIQRFALTFCRIVDLGIVTTLSLKEKQNNDCIFLENDGCSVYPVRPVQCRTYPFWETI
                     LENEQSWIEEGKQCPGIGRGSLISSDRILDAFFERRQNAPLSIDEIAALAALRNNWDI
                     "
     gene            18915..19931
                     /gene="mltG"
                     /locus_tag="PK105_03125"
     CDS             18915..19931
                     /gene="mltG"
                     /locus_tag="PK105_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOU60731.1"
                     /translation="MKANHIRSIMQNLLILFLILLLAIVILILTPPFRSESVEVKIKS
                     GSSLRDIAKELHNNHVIPSSRVFELYVRARGSERNIKSGTYRFMPLELPIVAANKLIK
                     GNYITQKVVIPEGRTARQIALILESNNVTRAEAFLEAVNDIELARSLGIPASNCEGYL
                     FPDTYFFDPQSDSRELVSQMVRGFFAAIERIEGSKTEILNPQSLHQRVILASIIEREY
                     RLAEDAPLIASVFLNRLRKNMPLQSCATVVYVMTEHLGKEAPSVVHYGDLKIQDPYNT
                     YINRGLPPGPISNPGEISLKAALFPPRTDYLYFRLEDINTGKHRFSHTLEEHNYSEII
                     PKGL"
     gene            19938..21728
                     /gene="dnaG"
                     /locus_tag="PK105_03130"
     CDS             19938..21728
                     /gene="dnaG"
                     /locus_tag="PK105_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOU60732.1"
                     /translation="MARISPQIRQEILDKTDFLSVYQEHVRLQKKGASFWGLCPFHSE
                     KTPSFSVSPDTGLFYCFGCHKGGSIIQFIMDIDKLSYTEAMQELAQRCGVQIQFEESE
                     KLGETEKERQTLLELNERLSKSFHWFLTNHDCGRIALEILHRRGLSDQLIERFSIGYA
                     PANRQWLYSFLKAKGYSDDFLKHSGLFSKKDLSYPLFADRIIFPIGDAKGRIIAFGGR
                     AMTEDGPKYINSSETAIFRKQETLFGFSQAIDSIKNTDNAIVCEGYMDALSFHAAGLT
                     NAVAPLGTAFTSQQAVVIRRRSSNIILCFDTDEAGLRAAERSCSIATAAGLETRVLLL
                     EEGKDASEILEKRGAGELTRMAKNTINAGQFLLARANQLFDISTMEGKAKAVSFLFPF
                     LDALDSDVKRQEYIKEIGRSIGVSTHAVAMDYAKAKVSGVRRVRDSYNLSNVPYSSSN
                     SARTSDLLFMAAVILSEDPFQFVDENLNIENLDDPRAKDLFSALQEAHKEGILDVEGI
                     LSLCTDEAAARFVREVDTSGELQGNLEKIIKDGLDQKKKAALERKRRELVARLKSFSA
                     DTDPLQEKLILEEIIKVDGELKTTGGDIDE"
     gene            21721..>23322
                     /locus_tag="PK105_03135"
     CDS             21721..>23322
                     /locus_tag="PK105_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU60733.1"
                     /translation="MNDMELDPAIAKLLEYAKEKKTISFDELSDMLPEQILNSDKMEA
                     IINLLVNNDVKIEEEVQIEEEALPKLTSIPLKSKMVSSEKEISSDDPVRLYLRDIGRE
                     NLLTAEQEVELSRMMEQGENIIKNTLRRSGMLIPEFHQIVVKATRKEPKDMSLQKKEN
                     TEKIAERRRLNQFYRELIDSSAADLRFYIESKKNIIAKGGNIFEDPHLIRIREPLLII
                     VNETDIHPEEITIFSEKFIVAARKIIRYRKEQDRIQGILQVSSSKDLRTLGRNLTIKE
                     KREEIEQRLGLSAEEIKDKIRQLQVNEKKLQDIENNFETPIDDIISMAREVNRGRKMM
                     KSAKDRLIKANLRLVVSIAKKYTNRGMQFFDLVQEGNIGLIKAVEKFEYRKGYKFSTY
                     ATWWIRQAITRSISDQARTIRVPVHMIEQINKVVRESRQLMQKLGREPTDEEIASQLG
                     WEIERVKAVKNVAREPVSLDTPIGEDEDSLLQDFIEDKEVENPAVQTNYNLLQEQLRM
                     VLSTLPKREQEVLRMRFGLDEGYSLT"
BASE COUNT         6757 a         4786 c         5048 g         6631 t
ORIGIN      
        1 cataggctat ttctttctgg tttgcaggaa catctacttc cattatatgt ccaaggaggc
       61 gctttttctc gtcaaacttg tttgcagtta ccataagacc tcgcgacaca gtaagccgtt
      121 cgttgccatc atagaatttg atgttaggaa agcgctcacc gattttcttc ttcaaatcta
      181 atttgatcac aggatcgact tccttgatgt cagagagcat tgaatagact ctttctgcta
      241 ctggttgagg ggcttcatta aggaattcct ctaaatcatt ttccttaaag agaatatttt
      301 gtacaagctt attgatcttt cggttttctg ttgtatcttt atggatatcg atttccttga
      361 aggttatatc aaggatgtgc aaaagagtaa gaatgatctt ttctcgcaca atgccaagct
      421 ccttcatcca agccttgtct tttaaatctt ttgcaagcca gatgaaggct tctcgcatgt
      481 ccttatagtt ctcgactata tacatagcca ttcgcttgag tttttcttca tgcccattgg
      541 actccagcgc atcaatcatc ttggcagatc tggcataagg caaaagctga atatagatgt
      601 ccggccaatt ttgaatatag agctttatat tctgaataaa ctggttctta agctctgtat
      661 ctttcaatcc aagatatatt ccaacgatat cgtcttcgat ctgctcaaaa aggtcgacaa
      721 atcgcatcgt cacgtattgc tgcattcttg ggtaacgccc ggacagctct tttaggagta
      781 ggtaagaacc tacgacaaat tcgttcacct gcgtcaactt taggaatgat acaaaatatg
      841 atagcatttc ctcaaaataa tcggtgtcga cttcctcgct catttcgagg tagtccctaa
      901 agtattgaac ccttgaaaag aaattctttt ccgccttgaa ttgtgtatag attttttctt
      961 cgaatgagag aggtctgtcc cgcacgatga aggtggaaat atcatcctgg gcattgccga
     1021 acataggatc tgtcttgagt atttctttgg cgcgagtgct ccatgaattc cactcagata
     1081 tggagagaat agatggtacg agctccgctt tgatgcgttt catatccgcg cgattatcga
     1141 agctcttgat tatgattttg attgtccagg ccggatctgt ctttactttt tctctgagct
     1201 tatcgcgagg ccaggttgct tttagaaccc atatatgatc cttggaaaga gtaattagac
     1261 tatcaaaagc cattttaagc gacatgtgat gattccgcga ttttgcgaaa tcaatgacaa
     1321 cttcatcgtt ggttatattt gaaatccttc caatattcca ggttctatgg aagacataat
     1381 ttcctctatc aaaagctatg cgtttttcaa agtcagggat ggtttcatgc agcgaacgat
     1441 aggattgtgt tagggttgaa agccttacac attcatcgaa catcgaatgt ccgctatata
     1501 tctttgaata acattcaagg aattctttgc gtaaggcagg attgcgctca tcatattcca
     1561 caataagctt cagtatgttc aatgcggtat cccacttttc gtttttctta taccaatcat
     1621 ataagtccat tagaattgtg gcggcttttt ccggcgaaac ctgttttgct attttcttct
     1681 gcgcgtgcag aaaaaagtca taatcctctg gcgaaagttg aatcagctta tgccataatt
     1741 cctttatatt tgaaaatagt cccttattga tgtagcgaat gagtgccttc ttatagaaat
     1801 cgatggcttc ttctattagg ccattctttt ccttccattc tgcaataagc tgtgctagtt
     1861 cggcttcttc agtgtcgatt cggacaagac tctcccatac cgtgtatcgc tgctcgattt
     1921 tgttctctgc agtatagcat tctgctaacc taacgagagc aatgcggttt tctccaaagt
     1981 ctagcatccg gttgcatata tactcgacaa tattgctttt tttgctttct gcaaataaat
     2041 ccattaactg aataagataa gtttcatcgg ccggctggcg cattatattt agcattccgg
     2101 cgacatatag cgcaataacg ctattttttg tttgagataa gtgctctatg caaagcttga
     2161 tgatatcatc agccacattc tgggcagtta tttcctcaat aaagcgatcg agttctttca
     2221 ggttggcgat agagtagttc gccattaggg ttctggtcca tttctcttca ttcaacatga
     2281 gctgtagttt ttcacttaat ggagactgat ccattctctt ctcctcttca tcctatatat
     2341 gattcataga tggattgatc gataatcttg atcttaagca gcaccgaatg caattctttg
     2401 gtatcctctc gggccttcag ataatccgcc atccaaaaat atttctttag aaggcggggt
     2461 ataaggctcg atctttgctg atgcgattct cttaattgaa tctcaagttg acgtgcagaa
     2521 gctgaaattc ggttatattc agcaccgctc aattctctct taatattgaa tacgccccag
     2581 atttctgcat aaactggtat ccattcagca atatcacttc cagaataccc tagtttttgt
     2641 atattttcaa taatcttaaa gatcaattcg gactctaaga aatcaatttc tatggcttgt
     2701 gcatcaataa aaaaggcttc tctgaataag gccttagaaa ggcgcgcttc tcctgacata
     2761 gcatatacat cagccaattc ggcaagaatg tttgcatcat tccgtctggc ttgggctgcc
     2821 cgctcaaggt gaataagagc agtttctata tctccggtgg cttttctggc tctgcccaac
     2881 ctcagatcta acaatggccc aaaggcctcc ttttcttcgt ccggaatggc aagatactga
     2941 tccagagcaa gtccaaatat gaatcgcctg taaacgtagc gagcagtatc gaatgagtct
     3001 cctatatgag aaaggaataa tatgaacttt ttccaggaac cacagagaaa atctccacgc
     3061 tctaaaagac ttcctgtttc tggcatttga gagatgagat ccttccagaa atgaaggcat
     3121 ttcatcgcaa agagaacttc cgggtgctca aaatccgctt tcaaagcatt ctcgagaaaa
     3181 gaatctgcgc tttgaatatc cgcatttttt aggctttcat aagccagata aagattttct
     3241 tctgctatgg ttgcttcttc ttttggacga ggctgatggg ccattagaat gttcttctcc
     3301 tttaactact attcaaatag tacccgagcc ttgaattttg cgtcaatggc tattctatgt
     3361 gctatttcca attagattga cattaggcct aaaaaaggag gctggaaaaa ctagacgaaa
     3421 caagttatca tatacagcat atgaaagaac caataattgc gccctcgatt ctgtcggctg
     3481 atttttatga cattggttca gctataaggc atgtggaaga gagcgggtgt ccttggctac
     3541 acctggatat catggatggg cattttgtgc cgcctatcac attcgggcat aagatgatcg
     3601 cggatattcg agcgaagaca gaattgttct tggatgctca tctcatgata gaagaacccg
     3661 agcgccacat aaatgcttat atagagagtg gtgctgatgc aattactttt catttagaag
     3721 cctgcgtgca ctcacaccgt ctaatccaga gcatatcacg tgcgggcatt aaggctggaa
     3781 tatcgatagt gccttcaacc cctgtctcta atatagtacc gcttcttgaa ttcttagacc
     3841 ttgtgcttgt tatgaccgta aatccaggtt atgggggtca gaagcttata cctttttgcc
     3901 tacaaaaagt ggaagaactc agaagaatta gagaacaaag gggcctccat ttcttgattg
     3961 ccatcgatgg tggagtaaat gcgaaatcga tctccgacat cgtgattcag cagccagatg
     4021 tcctcgtaat tgggagcgct ttttttaatg ctcaggatag aaagcagttt gttgccgatt
     4081 ttaggaatgc ctggacaagc ccaatcggaa atatcgattg acctggatnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc ggaaatatcg attgacctgg atatgggctt
     4261 tccaagattc atatattata aatattataa agggaagatc taagtatgaa aaagttgata
     4321 atgaaaatgt ttgcggttat tatgtttatt cttgcatctt tctcttatgc tcaagctaac
     4381 aaaacatcag atgaattctc ctatgcattc gatattttca gaaatggaga ttttgggcgt
     4441 gcaggagccg cttttgcaga tatatgccag accgagtcaa atatggaaat cttggctgac
     4501 agctgcttca tggctgcatt atcctatttt caggcagctg actttcccac ttcctatgaa
     4561 ttttgtgaaa aattcatgca atcattcccc tctcatccca atatgcctga tatgcaattc
     4621 ctgaaaggca gaattctgtt taagctagga ctctatgcgg atgctatatc ggttttcgac
     4681 attttcatta atagatatcc tgatagtccg ttttgttctt ccgtgttgtt ctggaaagga
     4741 gagagctatt accagcttgg ggatttcgct caagctctat ccatttttaa taaaatcaaa
     4801 gaggcatggc ccaacacgga agccacttct ctcaccgcct ggaggattac cgtgatggct
     4861 tcagaatcta gagaagccaa atatgctcgc cttgctgaat ttgagtctcg aagaaatatc
     4921 tatagagatg tgcagcagat gcaggaagat gtatatggag aacaacgaca tctaagagat
     4981 tacctatggt caaaagcaat cagaaagtat tctgcaaggc caaatataga aactcgagaa
     5041 ttgagcgcgg atgagacttc aaggaataat aggctcaata tgcttcttga agccaaagaa
     5101 agggctttga acctgctcat atggaaaata gacaactatc ttaaggatat aacaccatga
     5161 aaaaaccagt atgctttttc atcttattgt tcatagttat aggcgctcta tttgctattg
     5221 atatacgcaa cgggcttgtt agaattgcta ctgatgatct gacactgcag ccagcgcttt
     5281 atcgccttgt aaatatatct ggaaaatcga aatatgaacc attgtggttt agtggggatc
     5341 caaggacgag ttttattgta ctgaatatcg acaaccgcat atataggatg ggagcttcaa
     5401 aggagtataa gactagccag cgacaaataa agaatggcat tgaaatcgaa tatagatcgg
     5461 ttacaaatag attgactcag agattgcttt tttttacaag ccccttttct cgggtagcag
     5521 atggttttgt catcgaaata gaaatcgaaa actatacaac gcgagaaatg aaagtgatga
     5581 taaaagaagt atgcgatacg gttctcggcg agaaagaagg ctttcatttt gctacaagtt
     5641 taaatgccag ggttaccgac gaaatgattc ttgcatcaga ttccgatgaa aaatacgttg
     5701 tttctccagg acaggctgct tcccttgcgc tactcctcga tgaagatcaa aagccagata
     5761 tggttgtaat tgcaaactgg aagcgactga gcgattcaaa gttctattat gattctcaac
     5821 tcatgaaagg cttcactctc tcttctttct ctatcaatga ttccgctcta ggcctttatt
     5881 ggaatgagag aattatccca ccaaagggaa gcataaaagt ggcaagcact tggctcaccg
     5941 gtggatcagg tagcgaattc atcgattggc tatcgctaaa ttatcctttc ccccgccagg
     6001 aaccccaaca agtcttggag agtcctcctg tcgtattgga ggagcaatct atagtcaatg
     6061 cgccaatact cgatataaaa gccattcaag atcttatttc tgaaatagac agggctatag
     6121 ataatatcga aaacctcacc gacaatgaac tgcaggatat tatgacaaaa ctcaatttac
     6181 ttgaacaaag cgaagggctt gccgcccctc aatcgaacta agggtggtgc tttagatgag
     6241 agccgtaata cagcgagtat ccgaggcttc tgtgacatat agaacatcca gagaagagaa
     6301 attctgcgga agcattacta aagggatact aatctatctc ggaattgcat cggatgactc
     6361 agatgacgat gctgaatatc tcgctgataa aatagctcac ctccgcattt ttatggatag
     6421 taatgataaa atgaatcttt cgattctcga tcttggctat gaagcccttg taatttccca
     6481 gttcaccttg catgcggatg tccgaaaagg cagacggcct tcctattcta aggccgctgg
     6541 ttccgaaaaa gccttgatgc tattcgattt attctgcaca aaattgctgg agaacggagt
     6601 ccatatccaa actggcaaat tccaagagat catgcggatt cattcaatta atgatgggcc
     6661 tgtcacaatt ttgcttgatt cgaaaaaagc cttttgacat ttattgaggt tggaaacaaa
     6721 agaataggag cgctaaaata tcgctcctat tctttatttt gaacatggaa aatattacaa
     6781 gatttggtta tttattttta gaaccgcgtg gtctgccccg agggcgtttt gccttggacg
     6841 atgcggcctt tggaggtcta ccaggttttc ttgtagattt atgagcagcc tgagcatctt
     6901 caatcaccgc ctgtgcagca gcgagtctag agacgggcac cctgaaagga gaagaggata
     6961 catagttcat gcctactcga tgacagaatt ttaccgactc tggatctcca ccatgttcac
     7021 cgcagatacc taccttgagt ctaggcctcg atgagcggcc tttttctatt cccattcgta
     7081 taagcatacc tacgccatcc tggtcgatat tctgaaatgg gtctgctttg aaaatcgctc
     7141 ccttcttttc atcgatatag tctggcagaa acctaccggc atcatcgcgg gacaaaccga
     7201 gggtcatctg agtcagatca tttgtaccaa aggagaaaaa ttcagcgact tttgctattc
     7261 tatctgcgag caaagcggct cgtggaacct ctatcatcgt accgatcata tatttcaatg
     7321 gcacttctga tgccgctatt atggcagccg ctatttcacg tgctcttgac tcaagaattt
     7381 ttagctcttt gtcatctata accaaaggga tcataatctc tggaagaact tttataccat
     7441 tcttggtcat attgcatgcg gcggtgatta ttgccgtaac ctgcatatcc aggatttctg
     7501 gataggttat ggtcaaacgg cagcctctat gacctagcat tggatttagt tcatgcagtt
     7561 gctctactcg ctgaattacc ttttcaatag gaacaccaat tgcatcggca agctcgcgct
     7621 gaccttcttc atcgtgtgga acaaattcat gaagaggtgg gtcgattagg cggatagtta
     7681 caggtagacc atccatcact ctaaatatgc cctcgaaatc tttggtctga aatgggagga
     7741 gtttagccag ggctttttta cggctttcgg tatcatccgc tatgatcatc tcgcgaattg
     7801 caagaatacg ttcttctgaa tcgaaaaaca tatgctcagt tcggcatagt cctattcctt
     7861 cagcacccaa ctccctggcc ttttcagcat cataaggagt gtcggcattg gtgcgaactt
     7921 tgagctttct ggtcctgtct acccaggaca taagagtttt atacgcttta ggaggctcgg
     7981 gcttaacaag tttgagctgc ccaagatata catttccggt attgccatcc agagtgatat
     8041 aatctccttc ttttacgatc ttatctccaa cggcaaaatg gcggttttca tagtctattt
     8101 cgagcttttc gcagcccaca atacagcatt taccccatcc tcgcgcaaca acggcggcat
     8161 ggcttgtctt gccaccggta gctgtcagaa taccttgagc tgcatgcatg ccgcctacat
     8221 cttcgggact ggtttccttg cgaacaagga tgattttttc acccttttct tcccattcct
     8281 cagcgacctt tgcattgaat acaaccctgc ctactgcggc tcctggaaca gcatcgatac
     8341 cttgcacaag aaaagatttc ttaagcgctg tagcattcga tttatcgatc attggataga
     8401 aaattccctc tatatcagag gctttgatac gaagtatcgc ttcttcttta gtaatcagct
     8461 tttcttttac aagatctact gcgatgcgga aggctgcaat agggctacgt tttcccgtac
     8521 ggcactgcag catatagagc ttaccttctt ccacggtgaa ttccagatct tgcatatctc
     8581 tatagtgtct ttcaagcttt ctgcggatat cgacaagctg tttgtatact tttgggtcaa
     8641 gcttttcgag ctctgcaagc tttataggcg tcctaattcc cgcaacaacg tcctcgccct
     8701 gagcattaat gagataatca ccgtagaaga tattttcgcc agtatttggg tccctggtaa
     8761 aacatacgcc ggtgcccgaa ctgtcacctt tgttaccaaa aaccatctga acgatattta
     8821 cggcggtccc tttaattccc accagcttct ctacttttcg gtatgtcacg gctttttctg
     8881 ccatccaaga atcgaagacc gcattgatag cgccttttag ctgttcccat gagtcttgcg
     8941 gaaaatctgt cccaatatga tcgcgatata tcttcttgaa ggatttgata agctcaccga
     9001 gttcttcttc gttcacatcg gtatcgttta ctttttgttc ggagggtatt cctatgcgtg
     9061 gccttgtctt tgtctctttg atttcctcaa aggctttatc gaatatttcc ctatctattc
     9121 ccatggctat agagctatac atcataataa agcgcctata tgcatccaga gcaaagcgcc
     9181 tatttccagt cttagaagca agaccctcga ccgaagcatc ggtaagaccg agattcaaga
     9241 tagtctccat cataccgggc atcgaaacag gtgccccgga tcgaacagag acgagaagcg
     9301 gatcccttgg atcgccaagg gtcttttttg tcaccttttc tagattcttt atatgctttt
     9361 ggatctctgc ttcaagccct tccggccatt ttttcccatt tttgtaataa agctcacata
     9421 cttctgtggt gatagtgaag ccggcgggaa ctggaagtcc aattttagtc atctcgtgaa
     9481 ggcctgctcc cttgccaccg agcagctctt tcattgagcc atctccttcg gcaaggcctg
     9541 caccaaatgc atacacgaat tttcttcttg gcatgaggtt tttcctccga tatatagaaa
     9601 acgggctgac gaataagtaa ttcgctgcgg tggaaaacta atcgaaacgg caaaattagt
     9661 caagctatgt ataaataacg ttgttaatta tcttattatt ctgcttaaag gaattaagac
     9721 attgctttat atcgttatat ctatcgtcat ctccaaacga tttcgatttg gggggaagag
     9781 ctcacagtta aaagatcagc cattgcgcat tccaggatgg tctttttgcc agagcttgtt
     9841 ttacgaataa aatccccata tttaatagag acctcaggag ggggagattt tgcggtctca
     9901 atggtcagga taaatcgata agatgaaaac tgttcaaaaa tgtacttctt catatctgga
     9961 tcaagaggcg gaatgtcatt accaaaatcg aatttcaaag aatagctgcc attttcttcg
    10021 ctgaatctag tataacttcg ggtaggatcg aggtagttta taagattctc tggagaagcg
    10081 aaattcaacg caaaagaaat aattgtattg ttggcgttat ttgtttttga gtacgatttc
    10141 agggaaatac cttctgagcc ctgtaatcct cgctcgatat cttcgcgaga gagggcaagg
    10201 ggtagggaag gggtggtttc ttctgatccg aaatccacaa agtccttaga tatttgatat
    10261 acaacagaga atgttcccgc acctttggaa tcgattttgg ttgaagcttc aatcccgata
    10321 caagagatga aacataaagt cagagagata gtaaaagcca taaagagcaa tattttcccg
    10381 agacttttca ttggattagc cctctcatcc gtatatctcg taatgcatct gtacttcaaa
    10441 gagttctacc ggataatggt cctctatgaa cgttacagca tcttgcagta ctttttctcc
    10501 gtactgtgcg gaattagata ctagcgcagc tcctatttct gcaaaaacaa gtgaatcaag
    10561 caagtcagtt tcggcgcaag aaagtcggta tctgtgctga agtctgtcct ttattccgga
    10621 aactactctt cttttatctt tgatagtgga tgtctcattc aatttgagca ccaatcgcaa
    10681 cattgaaaca accattctat ataatattat cacttgagcc tgtccttgac agcccctttc
    10741 tacagggata ttgtccaagc catgaaaaga agactcatta cctcagccct tccatatgta
    10801 aacaatgttc ctcatcttgg caatctgatt caggtcttat ctgcagatgt gtttgctaga
    10861 ttctgtagac ttagtggata tgagactttg tatgtctgtg gtaccgatga atatggcacc
    10921 gcaactgaga caaaagctct cgaggaaaaa gtgagtccgc gtgagctctg tgataggttt
    10981 tatgcaattc accgcgatat ctatgaatgg tttgaaatat cttttgatac tttcggtaga
    11041 acatcgactc cagagcacac agagatcacg caatccattt ttctagatct agacaacaat
    11101 ggctatattt caagtcaatc catagatcaa ctctattgcg atacttgtgg ccgttttctt
    11161 gccgatcgat atgtaagagg aacttgtcca cattgtggtt atgatggagc acgtggcgac
    11221 caatgtgaga tgtgcgggaa attgctcgat ccaatggagc ttttgaatcc caaatgcata
    11281 agttgcggag gtactccaag accacgttca accagacatc tatatttgga tctgcctgca
    11341 atacttccta agctggaagc ctggatgaaa gacgcaagtg taaaaggctt ttgggcaaac
    11401 aatgcgatac agatgacaca ggcttggata cgagatgggc tgcatccacg cgctatcacc
    11461 agggatctaa agtgggggat tcccgtaccc aaagagggat ttaaggacaa ggtattctat
    11521 gtatggtttg atgcgcctat cggctatatt tcaatgacag caagattggc aaaggaaagg
    11581 gggttcgact ggaaaagctg gtggcagaaa cccgacgaag tagaactttt ccagttcata
    11641 ggtaaagata atattccgtt ccatactgtc attttccctt ccagccttct gggcaccgga
    11701 aaaaaatgga ctatgctgca tcatatgtcc agtaccgaat atctcaatta tgaagctggc
    11761 aagttttcca aatccaaggg tattggcgtt tttggaaacg acgctatgaa tactgggatt
    11821 cctgctgata tttggcgctt ttatgtattt tggaatagac cggagacttc agatcataca
    11881 ttcacatggg ccgatttcca ggaaaaggta aacggagaac taataggcaa tttgggaaac
    11941 cttgtaaata ggacactaac ttttatttcg cgctattaca aaggaattat ccccgatgga
    12001 actccagatg aagccttctg gaaggaggtt acggatcgcg aacaagagac ttcaaagctt
    12061 ctggaactgg ctgatcttcg agatgcattc agaaccattt tttccatagc agatttggcc
    12121 aacaagcgtt tccagtatga agagccatgg aaagctagaa tagacaatcc caaaaaagcc
    12181 agctccctta tgcgcgacct atgctatgtg ctgaaagatc tagcaataat gacctatccc
    12241 tatatgccaa ggacagccga gaaacttgca tcttttttag ggatagaaat aggacatggg
    12301 aagcttggct gggctgatct tggtctaaga ggagacctta aaagaataga aaaacatgag
    12361 gttcttttta atagacttga ggatgagctg attgcctccc ttcgtgctca atattctggc
    12421 tctcaaaaag aaagagaaga aatggcagag cagaatcgca aaagcgaggg gcagctccct
    12481 gtttctgaag aagaagatca caatgatcga ttagatcaga taatagatct atgcgtcgct
    12541 cgtattataa agatcgagcg acatcccaaa gccgacagac tatatataga gaccctggat
    12601 gatggcagtg gcttagaaag agttattgtt tcaggacttg tgccctttta tagagaagat
    12661 gagcttcttg gcaagaatat tataattgtt aacaacttaa agccagcaaa attgcgtggg
    12721 attgagagta aaggcatgct tctcgcggca tcgaccaaaa ccccagaggg aaacgatatc
    12781 gtcgaagttc tgaccgctcc aaaggcaaat cctggtgaaa gagtattgcc agaagatata
    12841 gacatggaaa atccggcaga gacggtacca aaggagagca tcgatatcga aacattttta
    12901 agaataccaa tgtatgcaaa agatggatat gcttggataa aaggccagcg tcttatggct
    12961 catggcgaac ctataaggct cgaaaaagtt atggatggga aaattggcta agtaaaaaac
    13021 caatgtccga tgtattagag cagagtgcca tggatgtttt accaagaata gaaataagcc
    13081 ccattctatc ggcaatcgat tgtttgcctg aatgctgtga tcagggcttt ttgcaatcca
    13141 agttctgggg actttttaaa tctcgcaccg gctggcaagc ctattcatgc acatactctt
    13201 tctataataa ggatttgagt ggtaggatcc ttgttttgaa gaggaaattt gcaaaacttt
    13261 tttcttttct ttatgttcct tttggagcag cggaattgag ttctattcca gagcgttgga
    13321 ttactctggc tgcacttggg cgagctttgg gcgaatcttt tggtggcaga gatgttttca
    13381 tacgtttcga tttgccatgg gagtgcgatg tagatccagc aaagactaag gaagaaaaaa
    13441 agcctgatat agaaaattct ttgggattcc atatttcggg cttgtataaa ggaaccgacg
    13501 ttcaggtgcc agacaccgtc atcttagacc tgcgagatga agaacaaaga atattagcta
    13561 aaatgaagcc taaatggcgc tataatataa gactatctca aaagaaaggc atttctgtag
    13621 cagacgaagg tttctccagt cttccattat ttatgtctct ttataaagaa acagctaggc
    13681 gagatagaat agctattcat ccggagagct actacaagac tctattcgaa acaagctctg
    13741 agatctcaga agttgctcaa gaaaattcta taactaccac caggccccgt ttgtctctat
    13801 atgttgctcg gcataaagat gaggttcttg cgtgcataat tgtcttgcat tttggaaata
    13861 tctcgaccta tctctatggg gcatcatcga gcgagaaacg aaatctaatg cctacttatg
    13921 cattgcaatg gcaagctatt ctcgatgcaa agagattcgg ggcagataaa tatgatttct
    13981 ttggtatacc gcccgatgat aaagatgcgt ctcatcctat ggccggactc tatctcttta
    14041 agacgggctt tgggggagaa ataatacatc gttatggagc ctatgatatc agtttaaggc
    14101 cttttctcta ttttgctttt agaacagcag aaaagatgcg aagcttctgg cacaaaagga
    14161 tcaaaaaatc tctgaataaa aagacagttg gactatctgg taaggccaca aaagagtcaa
    14221 caattcggaa tgtacatcaa gggcaggtat ccgaagagct agcttcttta agctccgatt
    14281 cagaaaacac gaaaccctcg aatccataaa gtcgcgcaag aggagatttc catatattgg
    14341 aagggagacc cgccttccag cagagctgct ctaagaaagt ttctttatcc catccaaatt
    14401 ctattggcac ttgtggaagc agtattccag atcttccatt agcatagatg agaagaccat
    14461 gtttccctat cacaatttcc tctggagaga tcgggtataa gggagagagg agggtgattt
    14521 ctatctcgat caatggaagc tcctcctcgt gcaagggcgg aaaacgtggg tcttcgaatg
    14581 cagccgcaag cgccatctgt tttatagtgc taggcaaatt agcgtcgccc tggattctac
    14641 caatacaacc cctaagcgat ccattttttt tcaatgtgac aaaggctcca cattttagag
    14701 atgtagaaag gtgcggagga tcgatttcct tttcgccttt caacgaagca tgtattgtga
    14761 ggcgcgcata attcaatagt ttcttttgat cctctggaga tattgcaaat tccataattt
    14821 acctcttaaa acctattgct ccatagccta ccactagctc atcacagtat tctctgtgtt
    14881 tcgaagaatt atcataatct agcaattctg ctcttgcctc tggaaatgca atcctgaagg
    14941 ctcgaataac tgcctctcca caaaaggaat tataacaagc tcttacgttt ccagtaaatt
    15001 cggcaactgg tctggaaaga gaatagatga aatccttaga aagcaaatca cattcttccg
    15061 gagaatcgct taccgcaaga tttgaagata gtacaaagac agcatagttt ccgatatatc
    15121 tgcttatttc ttcaaacaat ctcgtggcgg tctcaatggt cagaggattc ttacccgata
    15181 tgatgcaagg caatatgggc acccctggga aaaaatgaga tgcaaatggc aattgaagct
    15241 ctatcgagtg ttcttccaaa tgtggcagat catctttttt caacatgggt atcttgttaa
    15301 ggagataatc cctgaggttc aaatctatat tcaagcaggc acccggaata tcgaatcttg
    15361 tggattcagg aagaaaaaca ccatcttcat agggcagatg agaaggcccc ataatgacga
    15421 taattgaagg attcgaaccc agaattgcac cccacgattc catgctgatc ttcccgctgt
    15481 agagaaagct tccatgggga gcaataatgc caaatgcagg gcatggtatt cgctttgcat
    15541 atagactaag accgtctatt tcagacatta gcatctgcgg ttcagatgga tagaaaattc
    15601 ccgaaagcaa agggcttcgt gcgatttttt tttcgctcat tttagtgctc taatgtataa
    15661 gtatattgcc aaatctcgaa aatgcaagaa atgtgagtta tgattttttg gcgagaaatt
    15721 gattgcgcat agactgtcgt catattatta tttagctaag tggaaacagt tactcaagct
    15781 cttctttatg aagaaaaatt gcttagaaat cgccttcaga gagtcgagag cgaatgcgca
    15841 tcgagaccca agggtagtat tgtcctgaaa cgcagatata accaagtata tgcatattta
    15901 caatggaggg aaggcaataa agtatgcagc cggtatcttg gtagagcaga ttcctggcaa
    15961 tgcagatcga tccaagcaaa gatttcagaa aggcgaaaat acatggaaga ggcaaaggaa
    16021 ataagaaaga agcttgaggc gacaaggcat cttctagacg aagtgagaaa attcggttga
    16081 aaatgcagca cgaataggat acaatgtctg caatgaaaga tatttttata atgcaccacg
    16141 cggagtatga tacagctcca gactgtacat cagctgcgcc aactatagta aaaatattag
    16201 gagagcacac tgattttgcc gatggtcttg tgctttcagc ttctctttca tttgaagttc
    16261 gagtcgcgat atcttttaga aaagacaatt ctctccgttt ttatgcagct gatttcaatg
    16321 aacgtaagcg cgcaaatgtt gccacattta agtacaaaag agaagatcgc tgggctaatc
    16381 atgtaaagag tgtgtgcgat tatttttggc gcgtatttaa tcttgatcct cgtggcctca
    16441 atgtaaccat ccagagcaat gtgccactgg ggcttggttt gggtatcagc agtgcgataa
    16501 atatggcaac tgcgcttgac ttaaaaaccc tatacgattt agaccttaaa cttgaagaat
    16561 tagcctttca tgcttggaaa gcgcagagtt tattctttga gaaagaatta cctctaacaa
    16621 attatcttgg gattactgcg cctgcaggaa gaagtttttc gatcatagac ttgcatgata
    16681 aaaagcgcag aggagtgcaa ttcttacctg aacgctggga aatgcttctc actgattcca
    16741 aagtgcctcg ctcttcggtg gacgcagaac tggaacagcg aatcgcagat tgcaaagcat
    16801 gcctttcggt tcttgtcccc tatcataatc gttccattcg cgatatagcg cctcaagaac
    16861 tcgacgaatt gctaggaata gtgcctgaac gcaaacgccg acgatgtcta catgtgctag
    16921 aagaagtaag aagagtcatt gaagcggaag agagtctctc gcgtcaggat tatataggct
    16981 ttggtcgaat cgtaaacaaa tcccatgctt ctctaaggag cctttatgaa atttcctgtc
    17041 cagagataga ctggcttaca aagagagcgc ttgagctaga cgatgttctg tgttctagac
    17101 tcgttggtca agggtttggc ggaagcacgc ttacaatcct caataatgaa aaaaagcaag
    17161 cctatcgcaa caagctggaa gattatgaga gaatattcgg ctttaaacct attatccacg
    17221 aagtctcaac aggtgcaggc cttcgaattc tggagcattg attctaatat accaatccta
    17281 ttctagaaat ggagattaca tgagaattct gctgacaaat gatgatggta ttcaatcgga
    17341 tggccttttt gctttaatcg atacaattgc cgcaatgaaa gatttcaatg gaaaaagctt
    17401 gcaccaagta tgggtggttg cgcctgaaca tgagcgatcc ggcgtgagtc atgccatgac
    17461 tttgaaaaag ccggctaagc tgcgtaggct tggtgactcg cgctatagct gctctggcac
    17521 accggtggat tgcataataa ttgcgggact tgctattctc catgaaaaac cggatctagt
    17581 catttctggt atcaatagag gaccaaatct tggcacggat attgtatatt ctggaacctg
    17641 tggtgcagca cgacaagccg cattgcaagg aattccggca attgccatat catgtgcagc
    17701 tcattgtgat aatttggaat atgaaggtct aacatctttt ttatcagcta atctgaaaga
    17761 gcttgtggcc ttatggaagc ctgattcctt catcaatata aatggtcctt ctacaaaatc
    17821 gagagatcta aaggcagtat ggactgttcc aggaaaaaat aagtacattg acaacctcaa
    17881 gtgctttgac ggatcggatg gatatactta ttgttttctc accgaaggcg ccgaagaacg
    17941 aatgcccgat gctctctcag accatcatgc aatttctcag ggctatattg ccgtaggctt
    18001 agttgaagtt catcccaggg cattccacga gccatctctt aatgggcaat tcgtattctg
    18061 agagctatac tcagctgttc ctctttattc tcggaatcct caaaggcctt gaccaaacca
    18121 tcatctttcg tatatagtgc cgttcgttgt gcacgccatc ttagctcagt tgggagagcg
    18181 cgtgacttgt aatctcgagg tccccggttc gattccggga gatggctcga gggacatata
    18241 gtccatcaat ggggagattc ccgagtggtc aaagggggca gactgtaaat ctgttggctg
    18301 tagccttcga aggttcaaat ccttctctcc ccacaagggg ttgtcctgag agatgggcag
    18361 cccctttttg tttgttgtgc gcaaaggcct tttttgatta tctcttcaga ccacttgcag
    18421 gatggtggta atatgaatcc tgaattgcca ataaaagaca tctatttcca atgcatgcgc
    18481 tgcaatgctt gctgtagcgg ccaacctggt tttgtctggc tttctaagag agatcttatg
    18541 gctttaagcg aatattttga tctttcgata cagagatttg cactgacttt ctgcaggatt
    18601 gtcgatctag gcatagtgac tacattaagc ctaaaggaaa aacaaaacaa cgactgtatt
    18661 tttctagaaa atgatggatg tagcgtttat ccggttaggc cagttcagtg caggacatat
    18721 ccattttggg aaacaatctt agaaaatgaa caaagctgga tagaagaggg caaacaatgc
    18781 cccggtatcg gaaggggctc tctgatatca tcagatagga tattagatgc tttttttgaa
    18841 aggcgacaga atgctcctct gtcaatcgat gaaatcgccg ctctagcagc tttaaggaat
    18901 aattgggata tataatgaaa gcaaatcaca tcagatccat tatgcagaat ctcttgattc
    18961 tgttcttgat attgttgctg gctatagtga ttttgatttt gactcctcct ttcagaagcg
    19021 aaagtgtcga agtgaagata aaaagtggat ccagcctcag agacatcgcc aaagagctcc
    19081 acaataatca tgtcatacct tcctcccggg tattcgagct atatgtgcgt gccagaggtt
    19141 ctgagcgcaa catcaaatct gggacctatc gatttatgcc tttagaattg cctattgttg
    19201 cagcaaataa attgataaaa ggaaattaca taactcaaaa agtagtcatt ccggaaggca
    19261 ggactgcgcg ccagattgcc ttgattttgg aatcgaacaa tgtcacaagg gcagaagctt
    19321 ttttggaagc ggtcaatgat atagagcttg ctagatcatt aggcattccc gcttcaaatt
    19381 gtgaaggtta tctttttccg gatacatatt tctttgatcc acaaagcgat tcacgagagc
    19441 tcgtttcaca gatggtcaga ggtttttttg ccgcaataga aagaatcgaa gggtccaaaa
    19501 cagagattct gaatcctcag tctcttcatc aaagggtaat tcttgcctct attattgaaa
    19561 gagaatatcg attggccgaa gatgcaccat taattgcttc tgtatttctt aaccggcttc
    19621 ggaagaatat gcctcttcag tcttgtgcaa ccgtagtata tgtaatgacc gaacatctgg
    19681 gaaaagaagc gccttcggta gtgcattatg gagacctaaa gatccaggat ccatacaata
    19741 cttatatcaa ccgagggctt cctcctggac caatctccaa tccgggagaa atcagcttaa
    19801 aagctgcact atttccccct aggacagatt atttgtattt tagattggag gatattaata
    19861 ctggaaaaca tcgcttctct catactttgg aagaacacaa ctattcggag ataattccca
    19921 aggggctata gaaaagaatg gcgagaatat caccacagat taggcaggaa attctcgata
    19981 agacagattt cttatcagta tatcaggagc atgtccgcct tcagaaaaaa ggagcttctt
    20041 tttggggact ttgccctttt cattcggaaa agacgccgtc cttttctgta agccctgaca
    20101 caggcctctt ttattgtttt gggtgtcata agggtggttc aattattcaa tttattatgg
    20161 atatagataa gttgagctat acagaggcaa tgcaagaact ggctcaacga tgcggtgtgc
    20221 agattcaatt cgaagagtcc gaaaaactag gggaaactga aaaggaacgt caaactcttc
    20281 tcgagctaaa cgaaaggcta tccaagagtt ttcattggtt tttgacgaac catgattgtg
    20341 gtcgcatagc tttggagatt cttcatagaa gaggtttatc ggatcaacta atcgaaagat
    20401 tcagcatagg ttatgctcct gcaaatagac aatggctata ttcctttctc aaagcaaagg
    20461 ggtattcgga tgattttctg aaacatagcg gccttttttc aaagaaggat ctgtcatatc
    20521 ctctatttgc cgatcgaata atatttccca ttggagatgc taaaggaaga ataatagcct
    20581 ttggtggcag agccatgacg gaagatggtc caaagtacat aaacagctcc gagacggcaa
    20641 tctttaggaa acaagagaca ctatttggct tctcgcaagc gattgattct atcaagaata
    20701 cagataacgc cattgtatgc gaaggataca tggatgcgct ttcatttcac gcggctggac
    20761 tgacaaatgc agttgcccct ctggggacgg cattcactag tcagcaagct gtggttatcc
    20821 gccgaagatc atcgaatata atactctgtt ttgatacgga cgaggccggg ttgcgtgctg
    20881 ccgagagatc ttgctcgatc gcgacggctg ccggcttaga gactcgagtg cttcttctcg
    20941 aagagggcaa agatgcttca gagatattgg aaaagagggg ggcaggggag ttgacgagaa
    21001 tggctaaaaa tactataaac gctggacaat ttctgcttgc acgtgccaac cagcttttcg
    21061 atatatcaac aatggaggga aaggcgaaag cggtttcgtt tctgtttccc ttccttgatg
    21121 ctctcgattc cgatgttaag agacaagaat acatcaagga aataggaaga agcattggag
    21181 tcagcactca tgcggtcgct atggactacg ctaaagcgaa agtatccgga gtacgccgcg
    21241 ttagggattc ctacaatctt tctaatgtgc catatagcag ctcgaattct gcccgaactt
    21301 ccgatctttt atttatggca gcagtcattt tgtccgaaga tccattccag tttgtagatg
    21361 agaatttgaa tatagaaaat ctcgacgatc caagggcaaa agatctgttt tcagcccttc
    21421 aggaagcgca taaagaaggg attttggatg tggaagggat tctgtctctc tgtactgatg
    21481 aggctgccgc gcgctttgtc agagaagtcg atacctccgg tgaactacag ggcaatctag
    21541 agaagatcat aaaggacgga ttggatcaga aaaagaaagc tgctttagaa agaaagcgga
    21601 gagagcttgt agctcgtctc aaatcatttt cggcggatac agatccgcta caagaaaagc
    21661 tgatcctcga agaaataatt aaggtcgatg gagaactcaa aactacggga ggagatattg
    21721 atgaatgata tggaactcga tcccgcaatt gcaaagctac tcgaatatgc aaaagaaaaa
    21781 aagaccataa gttttgatga gctctctgat atgttgcctg aacaaatact caattcggat
    21841 aaaatggaag ctatcatcaa ccttctagta aacaacgatg taaaaataga agaagaagtc
    21901 cagattgaag aggaagctct tcccaaactc acttcgattc ccttgaagag caagatggtc
    21961 tcaagtgaaa aggagatatc aagcgacgac ccagttaggc tttatctgag agatatcggg
    22021 agagaaaatc tcttaactgc cgagcaggaa gtcgagctct cgagaatgat ggagcaaggc
    22081 gagaatataa tcaagaacac actacgaaga tccggaatgc ttatcccaga gttccatcaa
    22141 atagtagtta aagcgactcg caaagagcca aaagacatgt ctctgcaaaa aaaggaaaat
    22201 acagagaaaa ttgcagaacg aagaagatta aaccaatttt atagagagct catcgattca
    22261 agcgccgctg atcttaggtt ctatatcgag tctaaaaaga acataatcgc aaaaggcggg
    22321 aacatctttg aagatccaca tttaataagg atcagggaac ctctgcttat aattgtgaac
    22381 gagactgata ttcatcctga ggaaataaca atcttttcgg agaaattcat cgttgctgca
    22441 agaaagatca tccgctatag aaaagagcaa gatagaatac aggggatttt gcaggtctca
    22501 tcttccaagg atctaagaac ccttggaaga aatctaacta taaaagaaaa acgagaggaa
    22561 attgagcaac gccttggcct tagcgctgaa gagatcaaag ataaaattcg acagcttcaa
    22621 gtcaatgaga aaaaactgca ggatatcgag aataatttcg agacccctat cgacgatata
    22681 atatctatgg ctagagaagt gaatcgtggc cgaaaaatga tgaagagcgc aaaagatagg
    22741 ctaatcaagg cgaatctaag actcgttgtc tcgatagcga aaaaatacac taatcgaggc
    22801 atgcagttct ttgatctggt tcaggagggc aatatcggtc ttataaaggc cgttgagaaa
    22861 ttcgaatatc gtaagggcta taagttttct acctatgcaa cctggtggat acgtcaggca
    22921 ataactagat cgatttccga tcaagccagg accataagag tccctgttca tatgatcgag
    22981 cagataaaca aggttgttag agaatccaga cagctcatgc agaagctcgg ccgcgagcct
    23041 acggatgagg agatcgcatc gcaacttggc tgggagattg agagagtaaa agcggtaaaa
    23101 aacgtggctc gtgaacctgt ttcgctcgat acaccaatcg gagaagacga agattcactt
    23161 ctacaagatt ttatcgaaga taaagaagta gaaaatccag ccgttcaaac caattacaat
    23221 ctgctacagg agcaactgcg aatggtgctc tccacgctgc caaaacgcga gcaagaagta
    23281 ttgagaatgc gttttggact cgatgagggg tattcattga ca
//
LOCUS       DAOOWY010000022        22126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2348_length_22126_cov_13.027502, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000022 DAOOWY010000000
VERSION     DAOOWY010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 22126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22126
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2348_length_22126_cov_13.027502"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PK105_03140"
     CDS             <1..347
                     /locus_tag="PK105_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU60734.1"
                     /translation="AALVVLSELKRPGFLDEVDRKGRRLRALIESFHHPIVKDVRGMG
                     LMIGVGVKVDPHEVVSAAQSHGLLLLTAGDDTVRLLPPLVVTDKDIEEGAAALELALS
                     DVYTATNLVHMV"
     gene            411..1010
                     /locus_tag="PK105_03145"
     CDS             411..1010
                     /locus_tag="PK105_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60735.1"
                     /translation="MTPQELSKLDYEGASQMILAYTIDAKKYEKEIAALRSEAQDWKA
                     RAAEAQSKAVPELSQAALQRASQLESKALELSAELVAIRRDIEQLQEALPIIKAKQRR
                     VDPDQLLAELSMIVGLDNLGEQQGSSRRGDSTTSNADKDSKTIKDQDELNNKGSTSSA
                     DTTNRASLEKPINPPDEQSPVDHALDEALAELKKKMGLL"
     gene            1063..1911
                     /locus_tag="PK105_03150"
     CDS             1063..1911
                     /locus_tag="PK105_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOU60736.1"
                     /translation="MKIRHLPVLPKFPRPLLFAHRGLNRKYLENSMSSFEAAFHAGVP
                     GIELDVHLTADEKLVVFHDDTTGRIELKIQPEEPARNLCLEVSSLAELRSLSIGTHIP
                     LLDELFECFGDSLYYDIELKSRSKADTGLASKVAAAICSHNLESNCIISSFNPFALCH
                     FRKAKSTIPVGIIWSNSNELYWFLRHGEGALISKSDFLKPESSLFANMASRSPLHLLP
                     WFSGSQEKPIIPWTVNEPEKACDFIDQGVAGIISDIADEILIRFRAEREPAAAFDPPQ
                     KLSLKA"
     gene            complement(1838..3559)
                     /locus_tag="PK105_03155"
     CDS             complement(1838..3559)
                     /locus_tag="PK105_03155"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOU60737.1"
                     /translation="MKISLIQLNSTIGDFKGNTDRVVAMVDRVLSLFPALEKADLVVF
                     PELCLCGYPPMDLLDQESFCLGSLAALRRLQKLLPSDLAVGVGYVDRNRNRTGKSLVN
                     AYSVIQGGKVVFTQIKTLLPTYDVFDEARYFEPAKERNLFIQPWGKVGFAICEDFWWE
                     APDASQKYAIDPVKDLMDEGADIIVVTSASPFVAGKIRTRLNLAEKAVRAGSIPVLYC
                     NAVGANDSLVFDGRSFAMGEDASIRGICGWAEEALSYDSVSGKAKRVLFDPLLQKAEF
                     AQENQLPGSDSFEEIRRAIVVGIQDYLKKSGFSKVVLGLSGGIDSALVAVLAAQAIGR
                     QNVTCIAMPSRFSSEASLDDAVELCRRNKLRLERIPIEAPFTSYLDALSVPFAGKPYD
                     TTEENLQARIRGTLLMAWSNKFNALLLTAGNKSELATGYCTLYGDMNGSLAPIADLYK
                     TQVYGLTGYLNRMAAENGQTEPIPESIIAKAPSAELRYNQKDQDTLPEYKVLDQILQL
                     YIEENLSMEKIVNLGFDRAIVRKTLEMTGKAEYKRRQAAPAIKLSKRAFGVGRRLPLA
                     RVLHEIE"
     gene            complement(3556..4680)
                     /gene="lgt"
                     /locus_tag="PK105_03160"
     CDS             complement(3556..4680)
                     /gene="lgt"
                     /locus_tag="PK105_03160"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU60738.1"
                     /translation="MVAMLEFPKWLSPEIIPGLPFRWYGLMYLIAFGVTWLLFKRESR
                     RLGAPWTEDEAANFFIWGIVGILVGGRLAGTLIYEPTNYYWIKPWYIFWPFDESGAFV
                     GFQGMSYHGGFIGLIVASVIWCKIHKWNWFDWADLIAVSVPLGYTFGRLGNFINGELW
                     GKVSTVPWAMVFPNAERFSAKEQWVQEIARKVGIRIVSMNDMVNLPRHPSQLYEALLE
                     GIVLWLILWFFVRPRRSFRGQGVAVYSLGYGIARFFVEYFREPDSGLGYILALGDPDA
                     PTALFASPFNFSMGQILSFIMIVGALIFLFYARKHFKIKPGEPIFIDASRVGKVDNSA
                     RVKSQGNTDGTSGGNNARSTKYDASSARKLRKKIKSGGER"
     gene            complement(4680..5237)
                     /locus_tag="PK105_03165"
     CDS             complement(4680..5237)
                     /locus_tag="PK105_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60739.1"
                     /translation="MSHNFATVLTIEDRPTRLLFDMRRSLFLLIASILAFLRVFVIGV
                     VAILFGMSDYGGVTTALLRVFQLYNLLFVYIVIIQYVKAGAREYLRIPSLIVACVSPI
                     LSALALMAYIRNIGGSFPVDMAKAAISMVALILLDIVVFGLLVIDALKFQLPKAKNAE
                     NLENMENMEKTENMEMTHKDQHEVS"
     gene            5296..6765
                     /locus_tag="PK105_03170"
     CDS             5296..6765
                     /locus_tag="PK105_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU60740.1"
                     /translation="MKHIDMPAIRKQIIPFISLVLVLALSFLSVSIPELYAQQLKIPD
                     SKQYSLLLQNIYQFILQNYVEEPDPTKLYEGAVKGMLDSLNDPYSTYLDEDMMSDLMS
                     DTTGIYGGIGLYISKQATVANEDAPRYIEVVSPIEDTPAWREGIRPGDLIIKIDGEDT
                     SPMSTDKASAKIRGEAGTTVTLTFRRGTYEFEITFIRTKIEIPALKSAVIQTVNGNIG
                     YIRIIEWNPNTAPQMKTVLQDMKKQAIDKFILDVRSNPGGLLTSVVEVSDLFLSTNVI
                     VSTKGRSYSENYEYKAKPDLVIPLTDRMIVLIDQGSASASEIFAGAMKDTKRALLVGE
                     KTYGKGSVQQIFPLDKGGFKLTMAHYYTPSGIDIDKVGIEPDVKVPEPTLNEKELEEL
                     QRLYDAGEIARFLESNPKPTLTERKAFAAELAQKYKIQEIFLEKIIRDEAERSMPARI
                     FDLEYDIQLQKALDLIQSADFEQILESTKTLAQGRSTSN"
     gene            6781..7605
                     /locus_tag="PK105_03175"
     CDS             6781..7605
                     /locus_tag="PK105_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOU60741.1"
                     /translation="MRQIVLLGNQRGRESCSLDEKTSHYLINVRRMRKGDSFEAVDEA
                     GSRFSCTIVSDDSHAVKLEIKPIRFSRSLVRTPLNKAEEDKAKPSVAPTLHIALVQAL
                     PKGHKFDLIIRQAVELEVELIVPLITQHCVEQEPPERAKAKLERRKRIIREAIQQSGS
                     FVYTEILSAADLETLDAALNAKGFNKKDSVRILFHESEKEESQALHLLLSGDKDRVVL
                     AIGPEGGLSQNDCAAFIDLGFALHHARGPVLRVETAAAFAISAVRAIVFERGLWKV"
     gene            7593..8300
                     /locus_tag="PK105_03180"
     CDS             7593..8300
                     /locus_tag="PK105_03180"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOU60742.1"
                     /translation="MESVSIACAFLDGLRLDFPSQFDLFILPYLWDDDKNHQICFVDK
                     QNWMHARKRDEFGSMIASADLILPRDSGIAENVLAHGEKALRCWTVPFIYQVLLDVLN
                     ESMVEENTVGLENMKEQIRAYKPHKVLTLLLSSLEKRNGSVFLLGGNPITLIKAEKNI
                     RATYPGITIVGSMHGLYRPEEEPAIIEAMQKGAPQMILAGSPLPAEECWIPRHMNVTR
                     SGIFFYHKPIMKWISGR"
     gene            8436..>8620
                     /locus_tag="PK105_03185"
     CDS             8436..>8620
                     /locus_tag="PK105_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60743.1"
                     /translation="MKRLIYFDQDFRRIRPIIRLFSKYFDISVAGDLLELSIMIQRQK
                     PEILIVGLISSDSDFNAR"
     assembly_gap    8621..8720
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8721..9371
                     /locus_tag="PK105_03190"
     CDS             <8721..9371
                     /locus_tag="PK105_03190"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOU60744.1"
                     /translation="RALHEWSDRRSNPFLALNCAAIPYTLFESEMFGTERGAYTDASS
                     RSGIIEQANNGTLFLDEIGSLSPVSQPCLLRVLDNGEFRRLGSLHSRSSKFRLVTASS
                     RNPIELASQHEFRDDLLFRIANFVIETPPLRERKEDIPLLAQHFCDQYSIGRFNIGSD
                     ALDKMYSHNWPGNVRELFSVIMSACSLRKSGKICAKDIHFLPGIEKMSDQRKRNSR"
     gene            9469..10260
                     /locus_tag="PK105_03195"
     CDS             9469..10260
                     /locus_tag="PK105_03195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU60745.1"
                     /translation="MNNNNSEDIQGSIQLQNRVSTFSERLALVIGGSGGIGLAVSYEL
                     ASRGAALLIQGRRASEKLRVDSFPKAKSLNLIDLDFSSPVQFIQAIDLRLDEIGKEPD
                     IVVCAFGPFLEKPLKDCTAIDWEWLTLANLALPGALASRFLPGMMERKYGRFLFFGGT
                     RTDGIRSYKKTATYASSKTGLGVLAKSIAVQSASDNVAAIVVCPGPVETEYLDRETKI
                     RHASLTATGSLLKANTIAKIALDLIDSEPCIASGAVVNLDGGFAP"
     gene            10353..10895
                     /locus_tag="PK105_03200"
     CDS             10353..10895
                     /locus_tag="PK105_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU60746.1"
                     /translation="MPSGLLSRIQKISQQGSDALISSQVSTDFPGCILARFVGAVTEA
                     NLDAVAQSFDSILSEGIRYLVVDFSTIEDIGPAGMGLMLALRQKLRDRQGDLVLCGMR
                     PRMERMERILGLEGYFTTATDAKSALTGLKFALNGIYPLSVQCPACNSLIDIEKPGRG
                     RCQTCEAVITAFPDGTITLG"
     gene            10939..11334
                     /locus_tag="PK105_03205"
     CDS             10939..11334
                     /locus_tag="PK105_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOU60747.1"
                     /translation="MSALTRIESYLIDLGIEYQELSNNSWLIEDESKGYPKMVVSLSD
                     PLVIISANVMPVPKEKAEELFRTLLELNATDLLHGAYAISGDQIIVIDTLEYDTLDKE
                     EFLASIESISFALLEHYKKLANYIHKVEE"
     gene            11338..12048
                     /locus_tag="PK105_03210"
     CDS             11338..12048
                     /locus_tag="PK105_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOU60748.1"
                     /translation="MGMFDRLKTVISSNINALISKAEDPEKMLNQMIIDMNEQLIESK
                     KAVAMAIADEKRLEREMMENKAKAQEWEKKAMLAVRANRDDLAKEALLRKQEFESYST
                     QLAQQWEAQKQSVEKLKEALRQLQSKIEEANRKKNILIARAKRAEAQQRITQTMSSMA
                     GNKSAFETFERMERKIEEVEAQAEATKELEDTATGASLEKQFAQLESSPQAADVLLEE
                     LKKKMLTEDAGRNNSQSQ"
     gene            complement(12116..13390)
                     /gene="murA"
                     /locus_tag="PK105_03215"
     CDS             complement(12116..13390)
                     /gene="murA"
                     /locus_tag="PK105_03215"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOU60749.1"
                     /translation="MDKYIIEGRYPIKGTLTLSGNKNAALPCISASVLANGPVTLRNI
                     PAIEDVSVMFDVFRYLGGLVEQVDANSWCLDPRPISKYEVPEELAKKVRASILFAGPL
                     VARFGKAILPPPGGDTIGRRRIDTHIVALQELGARISLENTLTFSATKLVGVPVFLDE
                     ASVTGTENAIMAASLAEGTTIIMNAACEPHVQDLCRMLIQMGAEIDGVGSNRLIIKGA
                     ESLNGTDFAISADYMELGSFIGLAVVTGGDLRIDGVNADDLPPLKVGFSKLGVTWDMK
                     GSTLRPNSTVALRVIPDIGGQIPKIDDSPWPGFPADLTSIMTVVATQADGVVLIHEKM
                     FESRMFFVDKLISMGARIVLCDPHRAIVYGPSRLVGNVLVSPDVRAGMAMVIAALCAE
                     GTSIIHNVYQIERGYENLVQRLSAVGAHIKRE"
     gene            13455..14540
                     /gene="pyrC"
                     /locus_tag="PK105_03220"
     CDS             13455..14540
                     /gene="pyrC"
                     /locus_tag="PK105_03220"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOU60750.1"
                     /translation="MAKEIVRDKADIDRFYIPLPDDFHAHLRQGALLRASVRGHAGQF
                     GRVLLMPNTLPPIASSSLLEAYIAEVDQALAELPLEERFEPLYTFKLLPWMRSADIEA
                     LARAGSIAGKYYPSGSTTNAADGPRSFDEVEEVLNTMEAMGLVLCIHGEDPSAHILER
                     EQAFLPQVERLILRHPRLRVVLEHLSDAKSVHFVQQGSENLAATITAHHLLFTLDDVM
                     AEALNPHLYCKPVLKFERDRDALRMAVLSGSNKFFFGSDSAPHLREKKESAAAASGVY
                     SAPTALPALVELFNDLGGLDQLIPFLSENGARFYGLEAKPSFERQEQQNLDARSIAFE
                     RIPWTVPAMIDGIVPMCAGQRLSWRAV"
     gene            complement(14551..15675)
                     /locus_tag="PK105_03225"
     CDS             complement(14551..15675)
                     /locus_tag="PK105_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOU60751.1"
                     /translation="MDNKKYSRRRSALAAELMRKGLSAVIVSDFENGRDSSLRYLCGQ
                     PSDALLVVNKNATSVLIAWDVNMAHRMGDSNHILPFTDFGRQSAQALKAALDLLDLEK
                     GSKIALPASTPYPEYIELVQSLDAFDLICEKNGIDRFIKEMRSRKDAEEIILYQEICQ
                     YTDTLMDMVEKEVAAGTLSTELDVALFLERQARRMGAEGMGFESLVAGPERSFGIHAF
                     PTFTAGPFANKGLSILDFGLRFEGYTSDVTMSFVDNILSQEQKTMIDLVEEAHRIAVE
                     ACGPEVSLLKVATLVDEFFAKAGWFMPHSLGHGIGLDAHELPTLSMRAMPDDLLLPGH
                     IVTIEPGLYHPELGGVRLENDVLITDTGAEVITHSRIVRL"
     gene            16005..17459
                     /gene="mnmE"
                     /locus_tag="PK105_03230"
     CDS             16005..17459
                     /gene="mnmE"
                     /locus_tag="PK105_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOU60752.1"
                     /translation="MSRSYFDSHVPIVALATPEGRSALAIIRTSGEAAIDIVARCFSQ
                     SEALIGAAGYEAVHGWIIDPVTKERIDEVMALVYKAPHSFTGENAVEILCHGSIVVVR
                     RILDVLYAQGFSPALQGEFSFRAFIEGKTDLVRAEAINELSLASCEAARHDALLRLSG
                     VLSRRLKELRSMMLDALADIDARLDYSEDEGPEKIESWPTIICQAQSSIIELLKSYPG
                     GKLRQEGLLVVIAGRPNAGKSSLFNLLVQEERAIVSSEPGTTRDWLESWITMGGLAVR
                     LVDTAGLRNSKNEIEIEGVRRARAMLDQADLILYLVDGTVGLYEEDKTFMASYPGALF
                     LWNKVDRTDCLAVPSGWKATSAKDLASFGSLESMLVDELRAKVDEGYGMQGHEPQGRN
                     PPTCNSQDPTPLSRERQSYERQIRIVSERQKQLLYKAQEALNRAMEAHKAGVALDLVS
                     LHIQEAAESIGEISGDIASEEVLEHIFSSFCLGK"
     gene            17681..19561
                     /gene="mnmG"
                     /locus_tag="PK105_03235"
     CDS             17681..19561
                     /gene="mnmG"
                     /locus_tag="PK105_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOU60753.1"
                     /translation="MGDFEAIVIGGGHAGVEAALALARLGTKTVLITQNPDTIARMSC
                     NPAIGGLSKGNLVREIDALGGQMGILADATAIQTRMLNRSRGAAVQAPRAQADKALYA
                     KIARDALESQPNLSIFMDTVVDFILSKDVHPRIEGVVTERGNTIGARVVVLTTGTFME
                     AKLFIGAWSGPGGRLGEPAAMGLGTALREKGFPLGRMKTGTPARIKRESIDFSKLDVQ
                     HGDKAKIFFSFLEHDYERPDVPCYVIYTNEATHEAIRSGLDRSPLFSGSIVGKGPRYC
                     PSIEDKVMRFPDRERHQAFIEPEGLFTDEMYLNGLSSSLPEDVQERFYHSILGLENAI
                     IVRPAYAVEYDYIDPAALYASLESKLVEGLFIAGQTNGTSGYEEAAAQGLMAGINARR
                     KLDGSSPLILGRNEAYIGVLIDDLVTLSPKEPYRMFTSRAEYRLSLRHDTADLRLTPY
                     AVEMKLVDSRRRENFEHRVRTIEEVKALLEGHKINHEDIAQLPELEGHLGQALSDAIR
                     DPKVGSLYDDEIIALSRLAKILPQTASMQASGVITALLNERYKGYQEKENRLASRLAH
                     AEKLLIPRGFDYKAVKGLSQEASEKLSASQPLTLGQASRIPGVRKSDVALLYIALSKR
                     PR"
     gene            complement(19671..20312)
                     /locus_tag="PK105_03240"
     CDS             complement(19671..20312)
                     /locus_tag="PK105_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60754.1"
                     /translation="MAVIKSALELALERTKDLQLDESAQKLADARIEGRKAASQYLED
                     QTSVDFKAILNKLEPVQRQTFLTAAFEVLINFIQLPTNSVVETEKMESIGKAIVLLCG
                     LSPRFSSEREVKLAQQQARSLYQQILRFLSQYQEEMKRVEQAIRNQWAPKLRERERQL
                     AAALGQNVRIDPMSDPEFAEFYRKNIESMRNNYGKALEDAKKQLADTCGFESK"
     gene            complement(20348..21142)
                     /locus_tag="PK105_03245"
     CDS             complement(20348..21142)
                     /locus_tag="PK105_03245"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOU60755.1"
                     /translation="MEEKDRIADLPRNILLTLSIFFIVQCIIFLTFSLIVGFESQYWT
                     IFLPISTGFHILILIMLLLFRDDFYIESTGQKLDRINLANIITLSRVSSMPTLLVLVI
                     AAKEYSIRIPVLILIALIFLTDFFDGLISRKTNQVTKIGKILDSVSDYALLIVLSIIF
                     QYYLLIPGWLFWLIITRFGIQALFMAVLMIIAKRIEPKSTFMGKVTIASIMVLYTLEI
                     IKMILQASSHMVFRIAEWIVAIIIVIGIFDKIASFFDTIKSKPSAP"
     gene            21282..21914
                     /gene="pgsA"
                     /locus_tag="PK105_03250"
     CDS             21282..21914
                     /gene="pgsA"
                     /locus_tag="PK105_03250"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOU60756.1"
                     /translation="MTIADKITLSRMIMAPFFFFFVRAEFIPRQPAIIILWILFAWME
                     ISDLIDGKVARRSNQVGSFGKLFDPFADVISRVTYFLAFASIGIMPLWVLLIVMYREF
                     GILFLRALLALKGTAMGARPGGKLKAGTYMVAGLLSLCLYTVQTYASSMNSAIIDGFV
                     NHSDKMTEIASVLHSLTSISYVVVALATILSFIDYMIQFKKIYGQTNEKV"
BASE COUNT         6004 a         5165 c         5196 g         5661 t
ORIGIN      
        1 ccgcagctct tgttgtgcta tcggagctta aaagaccggg ttttttggac gaagtcgacc
       61 gcaagggccg ccgcctaaga gcactcatag agagtttcca tcatccaatt gtcaaagatg
      121 tgcgcggtat gggccttatg attggggtgg gcgtaaaagt cgatccacat gaagttgtaa
      181 gcgcggcaca gtctcacggg cttcttcttc taaccgccgg tgatgatacc gtgcggctct
      241 tgccgcccct ggtggtgaca gacaaagata tagaagaagg cgccgcggcg ctagagctcg
      301 cgctgagtga tgtctatact gctacgaacc ttgtgcatat ggtttgaagc agtaaggaat
      361 gcttactgta aagcgatgat tcaggtacaa tagaggcgag gtaaaaccca atgactccgc
      421 aggaactatc caagctcgat tatgaaggtg cttcgcagat gatcctcgcc tatacaatcg
      481 atgcaaagaa atacgaaaag gaaattgccg cgcttagatc ggaagcccaa gattggaaag
      541 ccagggctgc agaagcgcag agtaaagctg ttcctgagct ttctcaggcc gcgcttcaga
      601 gggcatcgca gctggaatcg aaggctcttg agctttcggc agagcttgtc gctattcgtc
      661 gcgacattga acagttgcaa gaagctcttc caattattaa ggcaaagcaa cgcagagtcg
      721 atcctgacca actccttgcg gagctctcga tgatagtcgg tctggataat cttggagagc
      781 agcaaggttc tagtcgcagg ggcgattcga caacatcgaa cgccgacaag gattccaaaa
      841 caataaagga tcaagatgaa ttgaataaca aaggttccac cagttctgct gataccacga
      901 accgcgctag cctcgaaaaa cccataaacc ctccagatga gcagagccct gttgaccatg
      961 ctctcgatga ggctctcgct gagcttaaga aaaaaatggg tcttctctga ctttgtctta
     1021 tgtcatctct ttgatcaatt atcaattata ggtaatagca ggatgaaaat acgccatttg
     1081 ccagtgttgc ctaaatttcc gcgacctctt ctttttgccc atcgagggct caaccgaaaa
     1141 tacctggaaa actcaatgag ctctttcgag gctgcctttc atgccggagt gcctggcata
     1201 gagctcgatg tccatcttac agccgacgaa aagctggttg ttttccacga tgacactacc
     1261 ggccgtatag agctaaaaat tcagcccgaa gagcccgctc gtaatttatg ccttgaggtt
     1321 tccagcctag ccgagctcag atcgctctcc ataggcactc atatacccct tctcgatgag
     1381 ctttttgagt gctttggtga ttctctatat tacgatatcg agttgaaatc tcgcagtaag
     1441 gcagatactg ggcttgcttc gaaggttgca gcagcgatct gttcgcacaa tctcgagagc
     1501 aattgcatca tctcttcctt caacccattt gctctgtgtc actttagaaa agcgaaatca
     1561 actataccag taggcataat ctggagcaat tcaaacgaac tctattggtt tctaaggcat
     1621 ggggaaggcg cgctcatttc gaaatcggat tttctaaaac ccgaatcgag cttgtttgca
     1681 aatatggcca gccgatcgcc gctgcatctg cttccatggt tcagcggctc gcaggaaaag
     1741 cccataattc catggacggt aaatgagccg gaaaaagcct gtgatttcat tgaccaaggt
     1801 gtggcaggaa ttatatcgga catcgcagat gaaatactta ttcgatttcg tgcagaacgc
     1861 gagccagcgg cagccttcga cccaccccaa aagctctctt tgaaagcttg atcgcaggtg
     1921 ccgcctgcct gcgcttgtat tccgctttgc cggtcatttc caacgttttt cgtactatag
     1981 ctcgatcgaa tcctaagttt acgatttttt ccatcgagag attttcttca atatatagtt
     2041 gtaagatttg atctagcact ttgtattctg gaagagtatc ctgatccttc tgattatagc
     2101 gcagttcggc gctcggagct ttggcaatga tggactcagg gatgggctct gtttggccat
     2161 tttcagcagc cattcgattg agatagccag taagcccata gacctgggtc ttatagaggt
     2221 ccgcgatagg agcaagggag ccgttcatgt cgccgtagag ggtgcagtag cctgtggcaa
     2281 gctcactctt gttgcctgcg gtgagcagaa gagcattgaa tttattcgac caagccatca
     2341 gcagagttcc tcgaatgcga gcttgcagat tctcttcggt ggtgtcataa ggcttgccgg
     2401 caaaaggtac tgaaagcgca tcaagatatg atgtaaatgg ggcctctatc ggtattcgct
     2461 cgagtcttag cttgttgcgc ctacatagct cgacagcatc gtctagagat gcctcgctcg
     2521 agaatcggct tggcattgca atgcaggtga cattctgccg acctatggct tgtgcagcca
     2581 agacagctac cagtgctgaa tctatgcctc cggaaaggcc caggactacc ttagaaaatc
     2641 ctgatttttt aagataatcc tgaattccaa cgacaatcgc tcgccggatt tcctcgaaac
     2701 tatctgagcc aggaagttga ttctcttggg caaactctgc cttctgaagc aaagggtcga
     2761 agagcacacg tttggccttg ccgctaacac tgtcgtagct cagtgcttcc tcagcccatc
     2821 cacatattcc gcgaatggaa gcatcttctc ccatggcaaa agaacgccca tcgaagacca
     2881 atgagtcgtt tgctccaacc gcgttgcagt agagcactgg aatagaccct gcgcgtacag
     2941 ctttttccgc caggttgagc cttgttctga tttttccagc aacaaaaggc gaagccgaag
     3001 taacgacaat tatgtcggcc ccttcgtcca taagatcctt taccggatct atcgcatatt
     3061 tttgcgaggc atcaggcgct tcccaccaaa aatcctcaca gatcgcaaag ccgaccttac
     3121 cccatggttg gataaaaaga ttccgttcct tggcaggctc gaagtacctg gcctcatcga
     3181 aaacatcata agtaggaagg agggttttga tctgagtaaa aacaactttt ccgccttgga
     3241 tgaccgaata tgcattgact agcgatttcc ctgttctatt gcgatttctg tcgacatagc
     3301 ccacgccaac cgccaaatcc gaaggcaata gcttttgtag ccttctaaga gcggctaaag
     3361 aacccaggca aaaactttcc tgatctagaa gatccatggg agggtaccca caaagacata
     3421 gttcagggaa gactacaaga tcagcctttt ctaaagcggg aaacaaggaa agcacccgat
     3481 cgaccatggc taccactcgg tcggtattgc ctttgaagtc tcctattgtg gaattgagct
     3541 gaattagcga gattttcatc gctctcctcc gcttttgatc ttcttgcgca gcttgcgggc
     3601 gctagaggca tcatattttg tggatctggc attgttgccg ccactcgtgc cgtcagtgtt
     3661 cccttgactc tttactctgg ccgaattatc tacttttccg actctcgatg catctataaa
     3721 tatgggctct cccggcttta tcttaaaatg ctttcttgca taaaagagga atattagcgc
     3781 cccaacgatc ataataaaac ttagaatctg gcccatcgaa aaattgaaag gagaagcaaa
     3841 gagcgccgtg ggcgcatctg gatctcctag ggctaagata taacccaaac ccgaatcagg
     3901 ctctcggaag tactctacaa agaagcgtgc tattccatag ccaagcgaat atacggcaac
     3961 accttggccg cgaaaactgc ggcgaggacg cacaaaaaac catagtatga gccataacac
     4021 aatgccctcg agcagagctt cgtaaagttg agaaggatga cgaggcagat ttaccatgtc
     4081 attcatggag acaattcgaa ttcccacttt gcgcgctatt tcctgaaccc attgctcttt
     4141 ggctgaaaat cgctccgcat tcggaaaaac catagcccaa ggtacggtgc tgacttttcc
     4201 ccagagctca ccatttatga aattaccaag cctgccaaag gtatagccaa gaggtacaga
     4261 aaccgcaatc aagtccgccc aatcgaacca attccattta tggatcttgc accagatcac
     4321 gctggcgaca attagcccta taaagccgcc atggtaagac ataccctgga aacctacaaa
     4381 tgcccctgat tcatcgaagg gccagaagat ataccatggt ttgatccaat agtagttcgt
     4441 tggctcatat atcagtgtgc cagctagtct acccccaact agaattccga ctattcccca
     4501 gataaagaaa ttcgccgctt catcttcggt ccaaggagca ccgagccgtc tggattctcg
     4561 cttgaagaga agccaggtca cgccaaaggc gattagatac ataaggccat accatctaaa
     4621 gggcagaccg ggaattatct ctggagaaag ccactttggg aattcgagca tcgcaaccat
     4681 caggagacct catgttgatc tttatgagtc atttccatat tctctgtctt ttccatattt
     4741 tccatatttt ccagattttc tgcatttttg gccttgggca gttgaaactt aagcgcatct
     4801 atcacaagca agccaaaaac aacaatatca agcaaaatca aggcgaccat ggagatagcc
     4861 gcctttgcca tgtccacagg aaaggacccg ccaatattgc ggatgtaggc catcaaggct
     4921 agcgcactga gaatcggaga aacacaggcc acgatgagcg agggtattct aaggtattct
     4981 cgcgctcccg cttttacata ctgaattatc acaatgtaga cgaataagag attatacaat
     5041 tggaaaactc gaagcaacgc ggttgttaca ccaccataat cgctcattcc aaatagaatt
     5101 gccacaacgc caattacaaa gacacgcaga aaagccaaaa ttgaggcaat tagtaaaaaa
     5161 agcgaacgtc gcatatcgaa aaggagtcta gtaggcctat cttctatagt caagacagta
     5221 gcaaaattat ggctcattgg ctatgataat gtgactcatg aaaacactac tctcggggaa
     5281 aaggagtcca acccgatgaa acatatagat atgccagcaa taagaaagca gataattccc
     5341 tttattagcc tggtgcttgt gctagctcta tcgttcctta gcgtttcgat tccggagctc
     5401 tatgcgcagc agctaaagat tcctgattca aagcagtatt cccttcttct gcagaatatc
     5461 taccagttta ttcttcagaa ctatgtcgag gaaccagacc cgaccaagct ctacgagggg
     5521 gcggtaaagg ggatgctcga ctcactaaac gatccctatt ccacatatct agatgaggac
     5581 atgatgtctg atctgatgtc ggacactacc ggtatctatg gggggatagg tctctatatc
     5641 tccaagcaag caaccgtggc aaacgaggac gcaccaagat atatcgaagt cgtatctccg
     5701 atagaggata cgcctgcatg gagagaagga attcgccctg gtgatctgat aatcaagata
     5761 gatggcgaag acacctctcc tatgagcacc gacaaggcct cagcaaagat ccgaggagag
     5821 gcgggcacaa ccgtcactct cacgtttaga agaggaactt atgagttcga aattacattt
     5881 attcggacta agattgaaat ccctgcactc aagagcgcgg tcatccagac ggtaaatgga
     5941 aatatcggct atattcgcat aatcgagtgg aatccgaaca cggcgccgca gatgaagacg
     6001 gtgctccaag atatgaagaa gcaagccatc gataaattca ttttagacgt gcggtcgaat
     6061 ccaggggggc tcctgacttc cgtagttgaa gtcagcgatc tttttctttc taccaatgta
     6121 atcgtttcaa caaaagggcg cagctactct gagaactatg agtacaaggc aaaacctgac
     6181 ctggtaattc ctctaacgga ccggatgatc gtccttatag atcagggttc agcttcagcc
     6241 tcggagatct ttgccggtgc catgaaggat acaaagaggg ctctcctggt tggagaaaag
     6301 acctatggaa aaggatctgt ccagcaaatt ttccctctgg ataaaggggg atttaagctg
     6361 acaatggcac attactatac accaagtggc atcgacatcg acaaggtagg aatcgagccg
     6421 gacgtaaagg tacctgaacc cacccttaac gaaaaagaac tcgaggaact tcaacgtctc
     6481 tacgatgcag gcgagatagc gcgatttctc gagagcaacc caaagccaac tcttacagaa
     6541 cgtaaggctt ttgcagcgga acttgcacag aaatacaaaa tccaggagat ttttctcgaa
     6601 aaaataattc gcgatgaagc tgagaggtct atgccagccc gaatctttga cctcgaatat
     6661 gatatacagc tgcaaaaggc tctcgacctc atccaatcgg cagatttcga acagatctta
     6721 gagagcacca agaccctggc ccaaggccgc tccacaagca attagataag gttttgcatt
     6781 atgcgacaga ttgttctgtt gggaaaccag cgcggccgag aatcttgctc gctcgatgaa
     6841 aaaacgagtc attacctgat aaatgttcgg cgaatgcgaa aaggcgactc ttttgaggca
     6901 gtggatgagg cgggttcgcg tttttcctgt accattgttt ccgacgattc tcacgcagta
     6961 aagcttgaaa ttaaacctat tcgcttttcc cgatctctgg tgcgcacccc cttgaataaa
     7021 gcagaagaag ataaggcaaa accaagcgta gctccaactc tgcatatagc cctggtccag
     7081 gcgctgccca aaggccataa attcgatctc ataattcgcc aggcggtaga actggaggta
     7141 gaactcattg ttccattgat cactcagcac tgcgttgaac aggagccacc agagcgagca
     7201 aaggcaaagc tcgaacggcg aaagcgcata attcgcgagg ctattcaaca gtcgggctct
     7261 tttgtctaca cagagattct gtcggctgca gatctggaaa cgctagatgc ggccttgaac
     7321 gcaaagggtt ttaataaaaa agactcggta agaattctat tccatgaatc ggagaaagaa
     7381 gaatcccaag ccttgcatct tctcctttcg ggtgacaagg atcgcgttgt tctagccata
     7441 ggaccagagg gtggcctttc tcaaaatgac tgcgcggcat ttattgattt gggatttgct
     7501 ctgcatcatg ccagaggccc agtattgaga gtggaaaccg ctgctgcttt tgcaatatcg
     7561 gcagttcgag ctatcgtttt cgagagaggt ttatggaaag tgtaagcatc gcttgcgcgt
     7621 ttctagatgg cttacgtctc gattttcctt ctcaattcga tctgtttata ttgccctatc
     7681 tttgggacga cgacaaaaat caccagattt gttttgtcga taaacagaat tggatgcacg
     7741 ctagaaagcg cgacgaattt ggatccatga tcgccagtgc tgacttgatt ttacctcgcg
     7801 acagcggcat tgcggagaat gtgctcgcgc atggcgaaaa agctcttcgc tgttggactg
     7861 taccttttat ttaccaggta ttattggatg ttctcaatga atccatggta gaagaaaaca
     7921 ctgtcggatt agagaatatg aaagaacaga ttagagctta taagccccat aaggttctta
     7981 ccctcctact atcttctctc gaaaagcgca atggctccgt gttcttattg ggtggaaacc
     8041 cgataacgct tatcaaggcc gaaaaaaaca tccgtgcgac ctatcccgga atcacaatag
     8101 taggaagcat gcatggctta tatcggccag aggaagaacc tgctatcatc gaggctatgc
     8161 aaaaaggggc tcctcaaatg attctagcgg gtagtcccct ccctgctgaa gaatgttgga
     8221 taccccgcca catgaatgta acccgaagcg gtattttctt ttatcataaa ccgataatga
     8281 aatggatttc cggtagataa aactttttgc cctctgtaga cataattaga tgttagctaa
     8341 tatttgaaat atattaggta aaaatatctt acttcgacaa agttgttctt taggccttga
     8401 aacatagaga tgcatttgtt acatctcaag caatcatgaa aagactaatc tattttgatc
     8461 aggactttag aagaatacgg cctataataa gactctttag caagtatttt gatatttctg
     8521 tcgcgggaga cttgctcgaa ttatccataa tgatacaaag gcagaagccg gaaattctta
     8581 ttgtaggact tatatcatca gattcagact tcaatgcgcg nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn cgcgctctgc acgaatggag cgacagaaga tcgaatcctt
     8761 ttttggcatt gaattgtgcg gcaattccat acactctttt tgaaagtgag atgtttggca
     8821 ctgagagagg agcctatacc gatgcttcct caagaagcgg catcatcgag caggcaaata
     8881 atggtacgct ctttctggat gaaattggta gcctttctcc tgtttcgcag ccatgtttgc
     8941 tgcgcgtact cgataatgga gaattccgta gactcggaag ccttcattct cgctcgtcta
     9001 aatttcggct tgttacagcc tcgagccgaa atccaattga gctggcctct cagcatgagt
     9061 ttcgagacga tctgctattc cgcattgcca atttcgtgat agagactcct cctctgcgag
     9121 agagaaaaga agacattcct ttgcttgcgc agcatttctg tgatcagtat tcaattggtc
     9181 gcttcaatat agggagtgat gccttggaca agatgtattc ccacaattgg cctggcaatg
     9241 ttcgagagct attctcggta attatgagtg cgtgctcgct cagaaagtcg ggcaagattt
     9301 gcgctaagga tatacatttt ttgccgggca tagaaaagat gtcagaccag cgcaagcgaa
     9361 atagccgtta aataagagct ttggccagaa ttgccgccag cggtcgctat ctaatgtcgc
     9421 tatctaatgt cgctatctaa taaggagaaa ttccagtatt ctcaagacat gaataataat
     9481 aattcagaag atatccaagg cagcattcaa ttacagaatc gcgtatcgac attcagcgaa
     9541 agactcgcct tagtcatagg cggctcaggc ggtattggtc ttgccgtgtc ctatgaactt
     9601 gccagcagag gtgcagccct gctaatacag ggacgccgtg cttcggaaaa attgcgagta
     9661 gacagctttc caaaagcaaa gagcctgaac ttgatagatt tggatttttc aagtcccgtc
     9721 cagttcattc aggccataga cttgcgccta gatgagatag gaaaggaacc cgatatagtt
     9781 gtctgcgcct ttggtccatt cctggaaaaa cccttaaaag attgtaccgc aatagattgg
     9841 gaatggctta cactagccaa tcttgccctg cccggagcct tagcatcgcg attcttgcct
     9901 ggcatgatgg aaagaaagta cggtcgcttt ctgttttttg gtggtacaag gacagatggc
     9961 atacgctcct acaagaagac cgctacttat gcttcttcaa agactgggct tggtgtgctg
    10021 gcaaagtcca ttgccgtgca aagcgcttcc gacaatgtcg cggctatcgt agtgtgccct
    10081 ggtcccgtag agaccgaata tcttgataga gaaaccaaga tacgccatgc atcgctgaca
    10141 gctaccggtt cgctcttaaa agccaataca atagcgaaaa tagctctcga tcttatcgat
    10201 tccgaaccct gtatcgcatc cggagctgtg gtcaacctgg acggcggttt tgcaccataa
    10261 gagttttgtg acataagggt tttgcgtagg cgggtgtttg acttttctgg gtgtttgact
    10321 ttttctaccc taacccatat tattgataca ctatgccatc ggggcttctt tcgcgaattc
    10381 agaagatttc tcagcaaggt tcagatgctc tcatctcgtc ccaggtatct acggatttcc
    10441 caggatgcat tttggcgcgc ttcgtgggcg cggttacaga agccaacctg gatgcagttg
    10501 cccaatcgtt cgatagtatc ctctccgaag gcatacgcta tctggtggtc gatttttcca
    10561 ctatcgaaga tatagggcca gcaggtatgg ggctcatgct tgccctcagg caaaaattga
    10621 gagaccggca gggcgatctt gtgctctgtg gtatgcggcc tcgcatggaa cgaatggagc
    10681 gaattcttgg actggagggc tattttacaa ctgcaacgga tgccaaaagc gcgctgacag
    10741 gtctcaaatt cgccctaaat ggcatctatc ccctctctgt ccagtgccct gcctgcaact
    10801 cccttataga tatagaaaag cccggcaggg ggcgatgtca gacatgtgaa gctgtcatca
    10861 cggcattccc ggatgggact attacgctcg gttgagcgca tagatctaag acaatagaag
    10921 tacaaggagg aggtagtaat gtcagcattg accagaatag aaagctatct catcgatctc
    10981 ggaatagaat atcaggagct ctccaataat tcctggttaa ttgaggatga aagcaaaggc
    11041 taccctaaaa tggtagtaag cctttcagac cctcttgtta ttatcagcgc caatgtcatg
    11101 cctgtaccaa aagagaaggc tgaagagctt ttcaggacgc ttctggagct caacgctacg
    11161 gacctcctgc acggcgccta tgccataagc ggcgaccaaa taatcgtaat agatacactc
    11221 gagtatgata cgcttgacaa agaagaattt cttgcatcta ttgaatcgat atcgttcgct
    11281 ttgctcgagc attacaaaaa gcttgcgaac tatattcata aagtggagga atgaattatg
    11341 ggaatgttcg atcgccttaa aacagtgatc tcatcgaata tcaacgcttt aatctccaaa
    11401 gccgaagacc ctgaaaaaat gctcaaccag atgataatcg atatgaacga gcagcttatc
    11461 gaatccaaga aagctgttgc tatggccatt gcagacgaga agcggctaga acgagaaatg
    11521 atggagaata aggctaaagc gcaagaatgg gaaaagaaag ccatgcttgc ggtccgtgcc
    11581 aaccgtgacg atctcgctaa ggaagctctg cttcgaaagc aagaattcga aagctattct
    11641 acccagcttg ctcagcagtg ggaagcacaa aaacaatctg ttgaaaagct caaggaagct
    11701 cttcggcagc tacaaagcaa gatcgaggaa gccaaccgca agaaaaatat ccttattgca
    11761 cgcgcaaagc gtgccgaagc ccaacagcgc ataacgcaga caatgtcgag catggcaggc
    11821 aataagagcg cattcgagac attcgagcgg atggagcgca aaatcgagga agtcgaagca
    11881 caagcggaag caactaaaga actcgaggac actgccacag gcgcgagcct agaaaagcaa
    11941 tttgctcagc tagaatcctc gccacaggca gcagacgtct tgctcgaaga gctcaagaaa
    12001 aagatgctga ccgaggatgc gggacggaat aattctcaga gccaataagg ctttttggtc
    12061 ttttttcaat tagcttcttc ctgatttggg ctgttttgct tttccgcctt cagtcttact
    12121 ctcgcttaat atgagctccg actgcggata gtctctggac gagattttca taaccacgct
    12181 cgatctggta gacattgtgg ataatgctag ttccttcggc gcagagggca gctatgacca
    12241 tggccattcc agctcgcaca tccggagaaa ccaatacatt gcccacaagc ctcgagggcc
    12301 catagacaat agctctatgc ggatcgcata gcactatcct cgcacccatc gaaatcaatt
    12361 tatccacaaa aaacattcgc gattcgaaca ttttctcatg tatgagcacc accccatccg
    12421 cttgtgttgc tactaccgtc ataatcgagg tcaagtcagc aggaaatcca ggccatgggg
    12481 aatcgtcgat cttgggaatc tggccaccta tatctggaat aacacgaagt gcaaccgtgc
    12541 tatttggcct caatgtgctc cccttcatat cccatgtgac gcccaatttc gaaaacccaa
    12601 ccttgagagg aggaagatca tctgcattga caccatcgat tcgtagatct ccgcctgtta
    12661 ctaccgcaag accaataaaa gagccaagtt ccatatagtc tgcgcttatg gcaaaatccg
    12721 tgccatttag ggattctgca cctttgataa tgagacggtt cgatcccact ccgtcgattt
    12781 cagcgcccat ctgaatgagc attctacaga gatcttgcac atgcggctca caagctgcat
    12841 tcataataat agtagttccc tcagcaagag acgcagccat tattgcattc tccgtgcctg
    12901 tcaccgatgc ttcgtctaga aaaacaggta cacccaccag tttggtcgcg gaaaaagtga
    12961 gcgtgttttc tagggagata cgcgccccaa gctcttgaag tgcaacaata tgtgtatcta
    13021 ttctcctacg ccctatcgtg tccccgcccg gaggaggtag aattgctttg ccaaagcgcg
    13081 ctacaagagg accggcaaat agaatcgagg ctcgaacttt tttagcgagc tcctcgggca
    13141 cttcatactt agagataggt cgcggatcta aacaccaact attagcatcg acttgctcta
    13201 ctaagccgcc tagatagcga aaaacatcga acattacgga tacatcttca attgccggaa
    13261 tgtttcggag ggttacaggc ccattagcca gaacagatgc tgaaatacag ggaagagcgg
    13321 catttttatt tccggaaagt gtaagtgttc ccttgatagg atatctacct tcgatgatat
    13381 atttgtccat agccttactg tatgcgctgc cgataagcag gtcaagcgcg attaggtttc
    13441 tcctggggga tatcatggcc aaagagattg ttagagacaa agcagatatc gatagattct
    13501 atataccatt acccgatgat tttcatgccc atctgcgtca aggagctctc cttagagcct
    13561 cggtgcgcgg acatgccggt caatttggca gagtacttct aatgcctaat accttgccgc
    13621 caatagccag ttcgagcctt cttgaggcct acattgccga ggtcgatcaa gccctagcag
    13681 aacttccttt ggaagaacgc ttcgagccgc tctacacttt caagctacta ccctggatga
    13741 gaagcgccga tattgaagcc ctggctcgtg cgggaagcat agccggaaag tattatccct
    13801 cgggatcgac aaccaatgcc gctgatggtc cacgttcttt cgacgaagtc gaagaggtgt
    13861 tgaataccat ggaagcaatg ggactagtcc tctgtataca cggagaagat ccaagcgctc
    13921 atattttaga gcgcgagcaa gcattcttgc ctcaggtcga gcggcttatt ctgcggcatc
    13981 cgaggttgcg tgtagtcttg gagcatctat cagacgcaaa aagcgttcac tttgttcaac
    14041 agggatctga aaatcttgct gcaaccataa cagcgcacca tctgcttttt accctcgatg
    14101 atgtgatggc ggaggcgctt aaccctcact tatactgcaa gccggtcctc aagttcgaac
    14161 gcgacagaga tgcgctgcga atggccgtgc tatctggctc gaataagttc ttttttggaa
    14221 gcgattcggc acctcaccta cgagaaaaaa aggagagtgc cgcagctgct tctggtgtct
    14281 attcagctcc gaccgccctt cccgcgcttg tagaactttt taacgactta ggtggtcttg
    14341 accaactgat tcctttctta tcagagaatg gcgcgcgttt ctatggcctt gaggctaaac
    14401 cttcttttga aaggcaggaa cagcaaaacc ttgatgcgcg ttcgatagcc ttcgaacgga
    14461 taccttggac tgtgccagcg atgatcgatg gcattgtgcc tatgtgcgct ggtcagagac
    14521 taagttggag agcagtctga cttcaagaac ctacaatctg acaattcttg agtgagtaat
    14581 tacttcggct cctgtatcgg ttatgagcac atcgttttcg aggcgtacac cgcccagttc
    14641 tggatgatat agtcctggct ctatggtcac aatatgcccg ggaagcaaga gatcgtctgg
    14701 catggctcgc atgcttagcg taggtaattc atgcgcatct aaaccaatcc catgaccaag
    14761 cgaatgtggc atgaaccatc cagctttggc aaagaattca tcaacaagtg tagcaacctt
    14821 caagagagat acttctggtc cacatgcctc caccgcaatg cgatgcgcct cctctacaag
    14881 atcgatcatg gtcttttgct cctgactaag aatattgtct acaaagctca tggtcacatc
    14941 gctggtgtag ccctcgaagc gcagaccaaa atcgagaatg gaaagccctt tgttagcaaa
    15001 aggacccgcg gtaaaagtag gaaaagcgtg gattccaaaa gagcgctccg gtccagcgac
    15061 aaggctttcg aatcccattc cctccgcacc cattcgcctt gcttgtcgct ctagaaaaag
    15121 agcgacatcc agctctgtag aaagagtacc agctgcaact tccttctcca ccatatccat
    15181 cagggtgtcg gtgtattggc atatttcttg atatagaatt atttcttctg catcctttct
    15241 tgatcgcatt tcctttataa acctatcgat gccgtttttt tcgcatataa ggtcgaatgc
    15301 atcgagagat tgaacgagtt ctatatattc gggataggga gtagaagcag gaagcgcaat
    15361 cttcgagccc ttctctaaat ccaagagatc gagcgccgcc ttaagcgctt gagcggattg
    15421 tctcccaaaa tccgtgaatg gtagaatatg attcgaatcc cccattcgat gagccatatt
    15481 aacatcccag gcgataagca cagatgtagc attcttattg accaccagca atgcatccga
    15541 aggttgaccg cacagatagc gcaacgacga atcgcgccca ttctcaaaat ccgatactat
    15601 cacggcgctt aggccttttc gcataagctc tgccgcgagc gcagaacgcc ttcttgagta
    15661 tttcttgttg tccatgcatg ctccttagga ttcaaagtaa accgacaata acagagcaat
    15721 tatcctccga cgcaaaggct ctgtccagtg agggttgact aaattacaag cgagctgaat
    15781 cagagttgta aaagcctgga gccacatact agattccctt tggagctgcc ttattatgga
    15841 gacattttgg attagggcag aaagggaaag actgaatagt cagttccttg ctattccctt
    15901 attcggcctt tttcctcctt cttatttcaa cttcttatac acaaggttcg tagcagtata
    15961 gactccttgt aataataggc gctgaaaggt tatcgtagtt ctgtatgtct cgaagctatt
    16021 ttgatagtca tgtgcccata gtagcgctgg ctactccaga gggccgatct gcgcttgcca
    16081 tcatccgaac ttcaggcgag gctgcaatag acatcgtagc tcgctgcttt tcgcagtccg
    16141 aggctcttat cggagccgca ggctatgagg cagttcatgg ttggataatc gatcctgtca
    16201 ctaaagagcg catcgatgag gttatggcgc tggtctataa agcaccgcat tcatttacag
    16261 gagagaatgc ggtggaaata ctatgtcatg gctcgattgt agtggttcgt cggattctgg
    16321 atgttctata tgcccagggg ttctctcctg ctttgcaggg cgagttttct tttcgtgcct
    16381 ttattgaagg caaaacagat ctagttcgtg ctgaggccat aaatgaattg agcctggctt
    16441 cttgtgaagc ggctcggcat gatgctcttt tgcggctttc cggggtgctt tctaggcgac
    16501 taaaagagct acgcagcatg atgttggatg cgctagcgga tattgatgca cggctcgatt
    16561 actcagaaga cgaaggacca gaaaagatcg aaagctggcc cacgattata tgtcaggctc
    16621 aaagttctat cattgagctt cttaaaagtt atcctggcgg aaaattgcgg caagaaggtc
    16681 tattggtggt aatcgctgga agacctaatg ctggcaaatc gagtctattt aacctgcttg
    16741 tgcaagaaga acgagccata gtgagttcag agccaggtac gacccgcgat tggctcgaat
    16801 cctggataac gatgggaggc ttagcagtga ggcttgtgga tacagctggt cttcggaact
    16861 caaagaatga gatagaaatt gaaggtgtgc gtcgagccag ggctatgctg gatcaggccg
    16921 atctgattct ctatctggta gatgggactg ttggcctcta tgaagaagac aaaaccttta
    16981 tggcctcata tcctggggct ctctttctct ggaacaaagt cgatcggacc gattgcttgg
    17041 cagtgccttc gggctggaag gcgacaagtg ccaaggatct cgcttccttt gggtctttgg
    17101 aatctatgct ggttgatgaa cttcgagcaa aggtcgatga aggctatggc atgcaaggcc
    17161 acgagccgca aggccggaac cccccgacct gtaattccca agatccaact ccgctaagcc
    17221 gggagcgaca aagctatgag agacagatca ggatcgtaag cgagaggcaa aagcaacttc
    17281 tttataaagc gcaggaggct ctaaatcgcg ctatggaagc ccataaggct ggggtcgcgc
    17341 tcgatcttgt ttctctgcat attcaagaag cggctgagtc cataggagag atttcaggcg
    17401 acatagcttc cgaagaggtg ctggagcata tattcagcag tttttgcctg ggcaagtgaa
    17461 caagccgcgc taaacgcaaa agaggaattg agagcgagag aagaaggcga gcgcgcaggg
    17521 agcgccgatg cgagagcaaa gagttcgcgc tttcacaaga tagccaatga agccctggcg
    17581 caagatagcg catggcaaga cttcaaaatg tagatttaag atcgcaaagg ggcaaaagtg
    17641 cgaaatcgcg cgaacgatta gacgattagg agagattgga atgggagatt tcgaggctat
    17701 tgtcatagga ggggggcatg ctggcgtcga agccgcccta gcccttgcaa ggcttggaac
    17761 caagaccgta ctcattacgc agaatcccga cactatagcc agaatgtcct gtaatcctgc
    17821 cataggtggc ctttctaaag gtaacctcgt gcgcgaaatc gatgcgctcg ggggacagat
    17881 gggcatttta gcagacgcaa ccgccatcca gacacgcatg ctcaatcgct cccggggtgc
    17941 ggcggtccaa gcaccaaggg cgcaagccga caaagcactc tatgcgaaaa tagccagaga
    18001 tgctctcgag agccagccga atctttcaat ttttatggat acggttgtgg actttatact
    18061 gtctaaagat gttcatcctc gcatcgaagg agtggtaact gagcgcggaa ataccattgg
    18121 agctcgggtt gtagttctga ctacaggcac ttttatggaa gcaaagctct ttatcggtgc
    18181 atggagtggg cctggcggcc gccttggaga accagcggca atggggttgg gaacagccct
    18241 ccgcgaaaaa ggatttcctt tggggcgtat gaagacagga acaccggcac ggataaagcg
    18301 agaaagcatc gatttttcta agctcgatgt gcagcacgga gacaaggcca agatattctt
    18361 ttcctttctt gagcacgatt atgagcgacc cgatgttcca tgctatgtca tatacaccaa
    18421 tgaggcgacc catgaggcaa ttcgctctgg attggatcgc tctccgcttt tttcgggcag
    18481 cattgtcgga aaagggccaa gatattgccc ttcgatcgaa gacaaagtaa tgagatttcc
    18541 ggatagagag cggcatcagg cttttataga gccagaaggc ctttttacgg acgagatgta
    18601 tctaaatggg ctttctagtt cgctaccgga agatgttcag gagcgcttct atcacagcat
    18661 tttaggcctt gaaaatgcga taattgttag acccgcctat gccgtcgaat acgattatat
    18721 cgatccagct gcgctctatg cctcattaga gtcgaagctt gtggaagggc tttttatagc
    18781 aggccagacc aatggaacct cgggatatga agaagctgct gcccaaggcc tcatggcagg
    18841 aatcaatgct cgccggaaac tcgatggttc atcaccgctt atccttggaa gaaacgaggc
    18901 ctatattggt gttctaatcg atgatcttgt tacgctttcg ccaaaggaac cttatcgcat
    18961 gtttacgagt agagctgaat accgactttc tctccgccac gatactgcgg atctgcgact
    19021 caccccctat gcagttgaaa tgaagcttgt tgattcgcgt cgtcgcgaga atttcgagca
    19081 tagagtaaga accatagaag aagtaaaagc ccttctcgaa ggtcataaaa tcaatcatga
    19141 ggatatagcg cagttgcctg aacttgaagg ccaccttggt caagcgcttt ccgatgcgat
    19201 tcgcgatcca aaagtcggtt ctctatacga cgacgaaatt atagcgcttt cgcgcctggc
    19261 aaaaattttg cctcagaccg ccagtatgca agcctcgggc gtcataacag cacttctcaa
    19321 tgaacgctac aagggatacc aagaaaagga aaatcgtctc gcaagccgtc ttgctcacgc
    19381 agagaagctc ttaattccac ggggttttga ttacaaagca gtcaaagggc tatctcaaga
    19441 agcctcggag aagctaagcg caagccagcc cctcacgctc ggtcaagctt cgagaattcc
    19501 gggagtccgg aaatcggatg ttgcgcttct gtatatagca ctcagcaagc gtccccgcta
    19561 gttaatctga tattgcgctt ctatgtatag ctattgcctc gagatggcct ttgattgaaa
    19621 tatcctattg ccgacaaaga tgatttatga tccagattgc cctgccttga ttatttggac
    19681 tcgaaaccgc aggtatctgc aagctgcttt tttgcatctt ccagggcttt tccatagtta
    19741 ttacgcattg attcgatatt cttccgatag aattccgcaa actcggggtc ggacatcgga
    19801 tctattctta cattttggcc taatgctgcg gctagttgtc gctctctctc tctaagcttg
    19861 ggcgcccact gattccgaat tgcttgttcg actcgcttca tttcctcttg atattgactc
    19921 aaaaaacgaa gtatttgctg ataaagggag cgtgcttgct gctgcgcaag ctttacttct
    19981 cgttcggatg aaaagcgtgg agaaagaccg catagcaaga ctatagcctt acctatggat
    20041 tccattttct cggtctcgac taccgagttg gttggcagct ggataaagtt aatgagcact
    20101 tcgaaggctg cggtcaaaaa agtctggcgc tgaacaggtt ccaatttgtt caatattgcc
    20161 ttgaaatcga ctgaagtctg atcttccaaa tactggcttg ccgcttttcg accctcgatc
    20221 ctcgcatcgg ctaatttttg ggcactttcg tcgagctgca ggtcttttgt cctctcgagt
    20281 gcgagctcta aggcactttt tatgacagcc attccaattc ctcctcgatt attgagaata
    20341 atataaatca gggtgcggag ggtttgctct tgattgtatc aaaaaagctg gcaattttat
    20401 cgaaaatacc aataacaatt ataatggcaa ctatccactc cgctatccgg aataccatat
    20461 gggaagaagc ttgaaggatc atctttatga tttcaagcgt ataaagcacc attatagatg
    20521 caatggtcac cttgcccata aatgtggatt ttggctcgat gcgttttgcg attatcatga
    20581 gcaccgccat aaacagcgcc tgaattccaa agcgcgttat aatgagccag aagagccatc
    20641 ccggaataag taaataatac tgaaaaataa tactcaggac aataagcaac gcataatcgc
    20701 taaccgagtc cagtattttg cctatttttg taacctgatt ggtttttctg gatataagcc
    20761 catcgaaaaa atctgtaaga aaaataagag caatcaatat aaggaccggt attcgtatac
    20821 tatattcttt tgcagctatt accagcacca aaagagtagg catggaactt actctgctaa
    20881 gcgtaattat attggcaaga tttattcgat cgagtttttg acctgtggat tcgatataga
    20941 agtcgtcccg aaaaagaagc aacattatga gaataagaat atgaaatcca gtgctaatcg
    21001 gcaggaagat agtccaatat tgcgattcga atcccacaat taaagaaaag gtcaggaaga
    21061 ttatgcattg aacgatgaaa aatatcgaca atgtgagcag gatgtttcga ggaaggtcag
    21121 caattctatc tttttcttcc atgaatattt ttatcctata ggatagccac atttattttg
    21181 cttgatatgc aaaggcgtac aatggccgaa atttacttat tatactatag cctcgattat
    21241 aatcttgaca agcttgatga ctaatccgta tgcttccttt gatgaccata gccgataaga
    21301 taacgcttag ccgaatgatt atggcgcctt tttttttctt ttttgttaga gccgaattta
    21361 tcccgcgtca gcccgctatt atcatactat ggatactatt tgcctggatg gaaatcagcg
    21421 atctcatcga tggaaaggta gccagaagaa gcaatcaggt aggatctttt ggcaagctat
    21481 tcgatccttt tgcggatgta atctctcgtg ttacatattt tttggctttt gctagcatag
    21541 gcataatgcc tctttgggtg cttctgatag tgatgtatcg cgaattcggc attctctttc
    21601 tgcgagcatt gcttgcgctt aaaggaaccg ccatgggagc ccgtcctggt ggcaaattga
    21661 aagcgggtac ctatatggtg gctggcttgc tttcgttgtg cttgtatact gttcagacct
    21721 atgcttctag catgaatagt gcgattattg acggttttgt gaatcattct gacaaaatga
    21781 cagagattgc gagtgtttta cattctttga cctctatatc ctatgttgtt gtagcacttg
    21841 ccactatttt gtcttttata gactatatga tccagttcaa gaagatttat gggcaaacga
    21901 acgaaaaagt atagagtttt ttttgaataa ttgccaagga acctcttgac caactctagc
    21961 ttgttcggtt atgttctcat ccgcgcccat cacttagtgc tgcatgttat gtaataattt
    22021 gcaataataa gcaaataaat gcgaaatatg caaaggcttt tgggttttgc aagcacttga
    22081 caactatcga tagatttggt agtttcatcg agcgctcttt aggtta
//
LOCUS       DAOOWY010000023        21142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2503_length_21142_cov_11.630009, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000023 DAOOWY010000000
VERSION     DAOOWY010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 21142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21142
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2503_length_21142_cov_11.630009"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PK105_03255"
     CDS             complement(<1..1169)
                     /locus_tag="PK105_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOU60757.1"
                     /translation="MANDIRILENKRVGEDFIPSRYLPPGENEYYLRDEQLRTLSKEP
                     ASFRPLSATELEVLIRNNNTCRDWSLFLVRDPFYPELLRNNIFFGLIRISRLEPVTLE
                     HHDFSTATGIYNSLVIACDIGDNCAISDCAYLSHYIIDDHCILINNAELQVSNHAKFG
                     NGIIMESEDESVRITLDVMNENGGRWIYPFNGMLPADAWLWAKFRDRDTLMERFAWMT
                     NTKFDRRLGRYGVIGRDSVLKHNRIIKDTYIGESAYIKGSNKLKNLTINSKSQAPTQI
                     GEGVELVNGILGYGCRVFYGCKAVRFVMCDNSALKYGARLIHSVLGENSTVSCCEILN
                     NLIFPAHEQHHNTSFLIASLIKGQSNLAAGATIGSNHNSRAPDGEIDAGRGFWPGL"
     gene            1289..1873
                     /locus_tag="PK105_03260"
     CDS             1289..1873
                     /locus_tag="PK105_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60758.1"
                     /translation="MDFVDSARVDTLVTESEKKVFEIFESMVKSTGQERKKSALALAN
                     LLGNPGEFSFYIDCTEDQRLIRVFHLLRVFRENMTLLINKTWADGSQNTTQELLLGEL
                     ARFIEEYRDFRIVSAFRTFVGIARQIPSLLFGSLGKANDFLEYAFRIDPKFGLFFWYI
                     GEIELQLRNIENLPEHRELFDLEVLIGAFVLSCF"
     gene            complement(1870..2535)
                     /locus_tag="PK105_03265"
     CDS             complement(1870..2535)
                     /locus_tag="PK105_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HOU60759.1"
                     /translation="MLFLQISHIPAMAIGFVSAMLPLQTSASSCGYAVAAGLINMMRS
                     SAYLAGFEDQELERETYANLYIPAFENDQALAQTYRKASPISLADIKAILARQGIDSM
                     AFKFSPGVLHRLVESLDTPLILHIRGRFSHFVLIIDAKLDAEEDAESAGILLFDPSCG
                     LALLSESELKSLVSGYCLLPIRYACKQGEKPMDIKDLKPSLDSLEAFLWNVFRILCSK
                     EEN"
     gene            complement(2625..4652)
                     /gene="fusA"
                     /locus_tag="PK105_03270"
     CDS             complement(2625..4652)
                     /gene="fusA"
                     /locus_tag="PK105_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU60760.1"
                     /translation="MDLGKIRNIGIMAHIDAGKTTTTERMLYYSGRTYKIGEVDDGEA
                     TMDWMDQEQERGITIQSAATTTFWRDHQINIIDTPGHVDFTAEVERSLRVLDGAIAVF
                     CAVGGVEPQSETVWHQADRYRVPRIAYINKMDRVGANFEAVLQDMKSKLGAHPVPLNI
                     PIGAESSFEGVIDLLEMQEIYWSEDGSVITKMPIAPERQEIAYHWRENLLDAVSSKSD
                     LITKLYLEAVAIPKDTLIAEIRKACIAQHFVPVFAGASRRNMGVQSLLDAIVDYLPAP
                     DEVGSIKAYHAKREQEIEIPCQISGYPLGLVFKVQYDREAGALCYVRMYSGTMHNSSV
                     VYNNSKKKRERITRLLRMHANKSEPIESVSAGDIAVFVGLKLAQTGDTLGTEGYPVLL
                     EKMHFPEPVISVAIEPRTLSDRDKLKEALDILAREDPTFAVHEDEETGQIIIRGMGEL
                     HLDVIVTRVIREFKVQAKIGNPQVTYRESISTKYRHSEKFHKIIAGKENTAGLTIEVE
                     PLPRGTGNQYKKLVRAPAVPQEIFEAIERGIMSAFSSGIQYGYPAVDIGVSLIDIEYN
                     ELTSTVFAFEACANMAYDAACRAANPILLEPIMKVDILTPKEFVGEVMSLVSQRGGFI
                     HGTETKIVAEIIHAEAPLATMFGFTTSLRSVSQGRASFSMEFSHFEPKREL"
     gene            4916..6973
                     /locus_tag="PK105_03275"
     CDS             4916..6973
                     /locus_tag="PK105_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOU60761.1"
                     /translation="MKKVELLAPAGSPEALDAAIGEGADAVYLGLRSFNARMRTTNFA
                     FNQFEAAVYACHNNSRRVYVTVNTVFEERESDRIYQLLEYLASVGPDGIIVQDAGVAK
                     IAHDHFPSLPLHASTQMNIASVAGCNQLSRAGFKRAVLARELSLEEIRQIRQYTSLEL
                     EVFVHGALCISASGLCLFSSYLGGKSANRGQCTQACRRLYESETGKGYYFSPDDLELI
                     EYIPDLIMAGVDAFKIEGRMKSAEYVGAVVSAYRLMLDNYEKDGEKAMLKAKAILQSD
                     FARRKTSFFISGKPEDYIHPEQSGGTGIALGRIRDARTIDGKRWALMNSREGLAERDS
                     VRIHRKDDSGRITTKILAIRYEVNGMLLLLDGDWRQNDELYLIQTASMTRSHKPVLPK
                     NLNRFHKFPSNHTAPRPAISRLDGRKLEISFEPGNYILVGKVAHLHAALTFRPKKAMI
                     LFNKLNAETMRLEEKSLPFKRDRLILWLDPYLAESDSTWLSAELDYWLEKGVSLVILN
                     NQAHFSMLRGKKVISIAGPWLYTFNKWALAYYIEQGVQAFIPPYEISRQDLYRLTEYL
                     PAQFFAPIVFAYPDLFRIRADLSRKYDNAKFMDREDNSFRLIGKRDYSVVVPDRPFSL
                     TDLVPNLKKQGFRRFIVDLSNAEPSKGLYRDIARAMEQGSPLPNTTRFNWKDGFWSEE
                     RMY"
     gene            7141..7923
                     /locus_tag="PK105_03280"
     CDS             7141..7923
                     /locus_tag="PK105_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOU60762.1"
                     /translation="MSESAGKAFDLLFYLAKAGETVSLARLSRETGMNKATALRYLNV
                     LESKGVVERSKLGWTLGLSLFELGSKVPVRQLVVDKVRPIIERLARETGESVNLAYLA
                     DETAIFLDRAEANRSLRMRSMPGDRLPLYCTGVGKAILSQLSEDRMAAILGPYPLEKY
                     TEFTCTDPDDIIKEVRRARELGYGIDSEEFEVGLTCYAMPLRIPSSDFVGAISISGPT
                     ARMKNPEIRDRFIYLLEQAIKDAIELLSPHYHLDIVSKKAKN"
     gene            complement(8009..8425)
                     /locus_tag="PK105_03285"
     CDS             complement(8009..8425)
                     /locus_tag="PK105_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU60763.1"
                     /translation="MNHLNSILVEGNLVRDPNFRSTPSGNQVCDFTVATNRNYKIADQ
                     KYENEVSYFDIEAWARLGSACAQNLKKGRGVRVVGRLKQDRWTDTEGKSHSRVKIVAE
                     HVEFKPMSKAAQEGAPKSETPFIEDSKRDAIETPVF"
     gene            8552..9949
                     /gene="purB"
                     /locus_tag="PK105_03290"
     CDS             8552..9949
                     /gene="purB"
                     /locus_tag="PK105_03290"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOU60764.1"
                     /translation="MENFDRFQSPFTWRYGSEKMREIWSERNKRKLWRHIWIALARAE
                     SRYGLVTPSQIAELESHADDIDFEEANRIEDSIHHDLMAELRVFASQCLEAGGVLHLG
                     ATSMDIEDNADVLRIRDSLSLVKEDFTKLLLALAGKIEDYASLPAMAFTHLQPAEPTT
                     IGYRYACYAQDLAEAYERLSYAIADIRGKGFKGAVGTSASYAELIGLENLDDFERVLS
                     EDLGIEFFDVSTQTYARVQDYRIISLLAEIAAILHKMAFDFRILQMPMLGELCEPFGE
                     HQIGSSAMPFKRNPIECERIESLARLFPSFANIAWDNAALSILERTLDDSANRRIILP
                     EAFLAVDELFSTMLHIIDGQIIDNEAIRCTLDAYAPFSATERVLMALGKRGADRQIAH
                     ERLRNMAMLAWQAVREGNSNPLKSLIESDEFIASFLSSDTIEKLFDVDIYIGAAEKRA
                     LAMAGRIRRLAMDEH"
     gene            9998..10651
                     /locus_tag="PK105_03295"
     CDS             9998..10651
                     /locus_tag="PK105_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU60765.1"
                     /translation="MLDFISKGGPILWVIMGLSLVALAIVIERLLYLKRISIDEEKLF
                     LRIKTSLMEGHFSEALAICDQNISPFSKLLKVGIENREKPEYLQRELLKDAASLESVS
                     LERGLTTLGTISNISTLLGLLGTVTGTMRAFGVLGKFGAVSDPAALASGVAEALITTV
                     GGLVVAIPVIMIYNYFVSRINLIMTRLETQVNNLVSFISSYGGKEPERAEEKPSKEA"
     gene            10651..11067
                     /locus_tag="PK105_03300"
     CDS             10651..11067
                     /locus_tag="PK105_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU60766.1"
                     /translation="MARNHKLTPKINFDLTPLIDIILQLVIFFMITTTFRTAPGINLQ
                     LPSSKTAQTISTPELRVVVVSSDEIYVDKTKTNIGSLQSVLSKRVSGSDAKQIRAVLE
                     GRAGAEYQLIISVLDALRMNGVENVGLITRKEKVVR"
     gene            11064..12092
                     /locus_tag="PK105_03305"
     CDS             11064..12092
                     /locus_tag="PK105_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOU60767.1"
                     /translation="MSEALTYEEREQRKRTIHSYIIAIAIYALLLGVGILFDILRPES
                     IDFSNKSLIVNIQGPVTNDIGKGSPIEKEESLVAEKPAPPPVQVPKPQADTSAKPKPA
                     EPLVPSPSVEAEPTPTPIVPETAAVQEPEIPWIPGERGPGSRVSSSESSVLAPGEGQV
                     PWGTGEAVRITKAEKGNTVETTLGGSSDSVGQSLYVPIYLSMPLPEIVPAELFNAIPP
                     EIVPPNTLIASAESRKRAFLNYYSWNGKEYRLTNPVPLEIRDKLWEMLEDAGYNVEKA
                     DYKAGRLLNPVVIGFSITKDRQLRGVEILQSSGDPEIDAAVLYGFKRASFWNKSGDTI
                     QGRFVYRF"
     gene            12171..15347
                     /locus_tag="PK105_03310"
     CDS             12171..15347
                     /locus_tag="PK105_03310"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU60768.1"
                     /translation="MIINLSSLIAAFSFCIYVFFIIFGISSRKERVSISFLIYMVMMA
                     LWSLGAFMAYSNIGFISLSDAIKIKLVGLLGAPFAIFATLLYFSKSQKKLFRSFLYIG
                     YGLYAYLLYLNFSGSIVENIWFEGLDYHYTLGQDALIVYSLAYVYLLLAILLLLKEQK
                     VNKNKFQKRALRLMLVGVVVLMVGVLAYLYEPVGRYPVDMIAAMINSSIIFFTIYKYK
                     LIHYSSTVFNIILGSLVAAFSSIIYMLFFIFLFKLDLYVPNSTLILLALCLGICSALI
                     FGPLRTAMQTLLEHISGGRSFLYYRELRNFSTKLTSIVKLEELSKLIVDNVISALKLE
                     WAFVLLNEYRSQNFRVIASHGLDFIQEQGNQNPSNQVLVPKSSPFVQAYQNRASQEEQ
                     VYSQWNIGIMLIKGQKHQTVLASLVLPLKFKERLNGFIILGPRLEKDYYNKYELDVLK
                     FLADQASVAMENAITYERLRQHQKRLQDANKQLINQRKFEAFFDGIATPISIQDINYN
                     IVTANFAAQRYFEKTQKELIGSKCYKAYFNRDRPCAECLAQDCLHTKLPFNGERQDNR
                     TQLTFSLNFYPIPTQEASLGAFIELFQDISKQKSEQEDTISEEKLAAIGSLVSTIAHE
                     INNPIGGILGTTDLILEEVTEGSTIRGYTEDIARYSKSALDFINSLMVYSKKTHTSLE
                     MLNIINVLENSLKMAMRSIDFGSIIVRKSYDSVKEILANSTELQQVFLSLIINAVQAM
                     RSDGVLTLSCRQEGDDVLVLIQDTGIGMRNQKFDRIVNDFLTVEEASSVSGMGLSFAN
                     HIVTKYGGRILLSSQPGKGTTFTVILPSAIQDKDRIWFINASEPNQFDDSYYIQRKVL
                     VGEKGYKEETIHRKCDETAFHILAYKGLQPIGTVSIHLSEATGHIPIEENFEIGPYLD
                     GAPYAEIDRLAVTKEERQGFTSFEIMVLSYLYVRGRGAKQIFIDTFADETGLIHMFKK
                     LGFEIIGGYSNPLPCTVLMMNHASTYEQETTKNRLFIKKTLSRLVPKIDFSADEMSYV
                     MKAIDEISAISPGKDSVDTKEISSYNLL"
     gene            15349..16410
                     /locus_tag="PK105_03315"
     CDS             15349..16410
                     /locus_tag="PK105_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOU60769.1"
                     /translation="MKPEDLAEGLNTILAHSPVFPDKCLIIDVVANPKAGGFSRIHHS
                     KKRMRELKEMVSRSLELPLRKAPLSLKMHLTERCGHAATIVQRILEHSPSNGKDSYHI
                     IITAGGDGTSLETAEQLTLLPESEKDRFGLVRLPFGTGNDGSEGRDLFVALERFLNPA
                     ILERKPALKITPSYEGGALPRYSFNIASIGLDAYVAEMTNRLKRHFPGDSYKFWVDIG
                     TMFYDRVYKVVNMGLRAWNRGRLVLDSELPRLLVAMGASGNRYYGSHKSILPNEHNCI
                     AISQTSLFRKLILKGSIENGKHEHIRELTHFTADRLQIDYHESIPLQCDGETTLLAKC
                     DFPLEMERLQNAYNVLVSV"
     gene            complement(16386..16955)
                     /gene="thpR"
                     /locus_tag="PK105_03320"
     CDS             complement(16386..16955)
                     /gene="thpR"
                     /locus_tag="PK105_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOU60770.1"
                     /translation="MRLFVALPFPAAVQNQIGTYCASIETAFRPARPSWVPPQNLHLT
                     LHFFGELEVEKAKELENLLSDAANLCPPLRITTEGLSVLPAMNRPRVLYLAARIEPSE
                     PLTILIDRIREIAAQLGAQTDARPWKAHLTLARLKLPYAPNLSSLPKPPMLSLYIDSF
                     ELLQSTLSPVGAIYSKIRRFALYTDTSTL"
     gene            complement(16963..17694)
                     /locus_tag="PK105_03325"
     CDS             complement(16963..17694)
                     /locus_tag="PK105_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOU60771.1"
                     /translation="MKNARATLSKLCEIALRRYDMIQPGDRILIAVSGGKDSCCLAWD
                     LSKKRQWLETPFEIAACHIVTDLEDFGLSQRRDDSWLALYMTKWGIPYTRLEIPVVGR
                     LKPGRSMNCYWCTTQRRSELMKFAKEHGFNKLALGHHMDDILETLLMNMLRKGEFATM
                     PPVMPYRKYPITVIRPLALCEEKQVLACADELGLSDYTYTCSFNLEGERKKARELLEH
                     LTEGSSQKKRNIFESMSRIRKDYLT"
     gene            complement(17818..18423)
                     /gene="udk"
                     /locus_tag="PK105_03330"
                     /pseudo
     CDS             complement(17818..18423)
                     /gene="udk"
                     /locus_tag="PK105_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537791.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0046108 - uridine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
     gene            complement(18467..19351)
                     /gene="ispH"
                     /locus_tag="PK105_03335"
     CDS             complement(18467..19351)
                     /gene="ispH"
                     /locus_tag="PK105_03335"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOU60772.1"
                     /translation="MQIVRARILGMCMGVRRADELAHRAAERAARTGRPVYSYGPLIH
                     NPQAVADLESLGVHVLDPSTIESELDELPNLRDGIVIIRAHGASLAAISRLHVLGARI
                     IDATCPRVIKSQGLARHYERLGWQVVLVGDSRHGEIAGILGHTLNALVVDSPETAPAL
                     ARSLRDKPCALIAQTTIRQEDYDTVIDIFQSYVRKMIAEKTICPATRERQQALAELST
                     QVDALLVVGGKNSENTKRLYRSAIGFGLPSWHIETADELKPEMVSYDRIGITAGASTP
                     DFIIDEIQEMLIRMAQNG"
     gene            19465..20409
                     /gene="arcC"
                     /locus_tag="PK105_03340"
     CDS             19465..20409
                     /gene="arcC"
                     /locus_tag="PK105_03340"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOU60773.1"
                     /translation="MAMRKTIVVALGGNAIIEEGTEGTIEQQFENTRKSMKAIVGMIA
                     EGHKVVLTHGNGPQAGVHLIRNEAASEQVPPSPLNVIVADTQGSMGYMIAQSLSNALR
                     AVGIDKDVVTVITQVVVDPNDPSMLNPTKFVGPFYKFEEVEKLRSRGWVIKEDPMRGY
                     RRVVPSPKPLDVIEKETIKDLIDDGKIVIAVGGGGIPVKRNPDGTLSGVDAVIDKDRA
                     SSLLASLIDADELIILTGVDKVAINFRKPDQRVFDHLSLRECEQYMAEGHFPKGSMGP
                     KIEAACDFIRRGGAKVIITSMENATAAVDGKAGTVISA"
     assembly_gap    20465..20599
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20600..20932
                     /gene="yajC"
                     /locus_tag="PK105_03345"
     CDS             <20600..20932
                     /gene="yajC"
                     /locus_tag="PK105_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOU60774.1"
                     /translation="VFVIFYFLIIRPQNKKQKKMQQMIAGVKKGDKVVTIGGIHGVVT
                     SVRDSTVLIKVDDGARIEFSKSAIASISPAKTEERAEDKYDEKIEDKPEDSAESTRKV
                     EKEKTEDK"
BASE COUNT         5562 a         4809 c         5002 g         5634 t
ORIGIN      
        1 agccccggcc aaaaacctcg ccctgcatcg atttctccat cgggtgcgcg cgaattatgg
       61 tttgagccga tcgtagcgcc cgcggcgaga ttgctctggc cttttatgag gcttgctatc
      121 aagaaactcg tgttatgatg ctgctcatgg gctggaaaaa tgaggttatt gagaatctcg
      181 cagcaggata cggtgctgtt ctcgccaagg acggagtgaa ttagtcttgc tccatatttc
      241 aaggctgagt tatcgcacat gacaaatcgc accgccttgc atccatagaa cacgcggcag
      301 ccatagccca ggattccgtt gacgagctct acaccttcgc caatctgagt cggtgcttgg
      361 ctcttggaat tgatggtgag gtttttcagc ttgtttgagc cttttatata agcgctttct
      421 ccgatatagg tatctttgat gatgcgattg tgtttgagca cgctgtcacg gccgataact
      481 ccgtatctac ccagcctgcg atcgaatttc gtgttggtca tccatgcaaa acgctccata
      541 agagtatctc tatcgcgaaa tttcgcccag agccaggcat ccgcaggaag cattccattg
      601 aatggataga tccagcgacc accattctca ttcataacat cgagcgttat tcggaccgac
      661 tcatcttcgc tttccattat aatgccattg ccaaatttgg cgtgattaga gacttgcaat
      721 tccgcattgt tgatgagaat acagtgatca tcgatgatat agtgtgaaag ataggcacaa
      781 tccgaaatgg cacaattgtc cccaatatcg caggcaataa ccagcgagtt gtatattccg
      841 gttgcagtgg agaagtcatg gtgttccaag gtgacaggct caaggcggga aatccgaata
      901 agcccgaaga atatgttatt cctcaaaagc tcgggataaa atggatcccg cacaagaaac
      961 aaagaccagt ccctgcatgt gttattgttt cttataagca cttctaattc ggtggctgaa
     1021 agtggtctga aagatgcggg ctccttacta agagttctaa gctgttcatc gcgtagatag
     1081 tattcatttt caccgggagg cagatatcga gatggaatga agtcttctcc cactcttttg
     1141 ttttcgagaa tcctaatatc atttgccatg tttcaagtat gaaatactct ttgttcttgt
     1201 gcaactggac gaatagacca tcgagcgaaa agttagagct tctcttatcc cccttgacca
     1261 tttcatgcct tttcgtcatg gtattgccat ggattttgtc gattccgcca gagtggacac
     1321 tctcgtaacc gaatccgaga aaaaggtctt tgagattttc gaatcgatgg taaaatccac
     1381 agggcaagaa cgaaagaaat ccgcgctggc tctggcaaac cttttaggaa acccagggga
     1441 attcagtttc tacatcgatt gtacagaaga tcaacgccta atacgtgtgt ttcatctttt
     1501 gcgcgtattt cgagaaaaca tgacacttct aataaataag acctgggcag atggaagcca
     1561 gaacaccaca caagaactac tgcttgggga gcttgcccgg ttcatcgaag aatataggga
     1621 ttttcgtatt gtctcggctt ttcgtacctt tgtaggaatt gctcgccaga tcccatctct
     1681 cttatttgga tctctcggaa aggcgaatga ttttctcgaa tatgcatttc gtatagatcc
     1741 gaaatttggt cttttcttct ggtatatagg cgaaatcgag cttcagttga ggaatattga
     1801 gaacttgccg gaacacaggg agcttttcga tcttgaggtt ctaattggag cctttgtgct
     1861 ttcctgtttc tagttttctt ctttgctgca taagattcgg aatacgttcc ataaaaaggc
     1921 ctccagagaa tcaagactcg gcttgaggtc tttgatgtcc ataggttttt ctccttgctt
     1981 acaagcgtat cttattggaa gaagacaata tccagaaacc agactcttta gttcactttc
     2041 actcagcagc gctagcccgc aggaaggatc aaagagaaga attcccgccg attcggcgtc
     2101 ctcttcagca tctaattttg catcaataat caggacaaag tgagaaaatc ggccgcggat
     2161 atgaaggatc aggggagtat ctaaggattc aacgagccga tgaaggactc caggagaaaa
     2221 cttgaatgcc attgaatcga ttccctgcct tgcaagaatt gccttgatat cagctagaga
     2281 aattggtgat gcctttctat aagtctgggc tagagcttga tcattttcaa atgctggaat
     2341 atataaattt gcatatgttt ctctctctag ctcctgatcc tcgaagcctg ccaaataggc
     2401 actgctccgc atcatgttga ttaagccagc cgcaaccgca tagccgcaac ttgatgcgct
     2461 tgtctgaagc ggaagcatgg cagagacgaa tcctattgcc atagcaggaa tatgtgaaat
     2521 ttgcaagaaa agcatctcgc gcctcctccc cgaagagaag acgcgaacaa aaattcatct
     2581 gcttcaaata ttagaggacg aataccctct tggtaggata ttgcctataa ttctcgcttg
     2641 ggctcgaaat ggctgaattc catactaaaa gaagctcgac cctggcttac agatctgagg
     2701 ctagttgtaa agccgaacat agtcgcaagc ggggcctcgg catgtattat ctcggccaca
     2761 atctttgtct ccgtaccatg aataaagccg ccgcgctgag agacaaggct cataacttct
     2821 ccgacgaatt cctttggagt cagaatatcg accttcataa tgggctcgag caaaataggg
     2881 tttgccgctc tgcaggctgc atcgtaggcc atattggcgc aagcctcgaa ggcaaataca
     2941 gtggatgtga gctcgttata ttcgatatct ataagtgaaa caccgatgtc cacagctgga
     3001 tatccatact gaattcccga agagaaggct gacattatgc ctcgttctat agcttcgaag
     3061 atttcctgag gcacagccgg agctcgcaca agtttcttat actgattgcc ggttcctctt
     3121 ggaagcggtt ccacttcgat ggttaagcca gcagtatttt ctttgcctgc aatgatttta
     3181 tggaacttct cgctatggcg atatttggtc gaaatcgatt ctctgtaggt gacctgagga
     3241 ttgccgatct tggcttgaac cttgaattct cggataacgc gcgtgacaat cacatcgaga
     3301 tgcaattctc ccatgcctct aatgatgatc tggcctgtct cttcatcctc atgaaccgca
     3361 aaagttggat cttctcgggc aagaatatcg agggcttctt ttaacttgtc tcggtcggaa
     3421 agggttctgg gttcgattgc aaccgatatg acaggttctg ggaagtgcat cttttcgaga
     3481 agtactggat aaccctcggt accaagggta tcgccggtct gggctaattt gagccctaca
     3541 aataccgcaa tatctcctgc gctaaccgac tctataggct cgctcttatt ggcgtgcata
     3601 cgcagaagtc tggtgatgcg ctcacgcttt tttttggagt tgttatacac aactgaggaa
     3661 ttgtgcattg tacccgaata catacgcaca tagcaaagag cgccagcctc ccgatcgtac
     3721 tgcaccttga ataccagtcc gagaggatag ccgcttatct gacatggtat ttctatctct
     3781 tgctctcttt tggcatggta agccttgatc gaaccaactt cgtcaggggc tggcagataa
     3841 tcgactatcg catcgagaag agattgtacg cccatgtttc tgcgcgatgc tccggcaaat
     3901 acagggacaa agtgctgagc tatacaggct tttcgtatct ctgcaatcag ggtatctttt
     3961 ggtatggcaa cagcttcgag atacaacttg gtgataagat cggacttgga cgaaacagca
     4021 tctagaagat tttctcgcca atgataagct atttcctgcc gctctggagc aatgggcatt
     4081 tttgtaataa ccgaaccatc ttcgctccag tatatctctt gcatttcgag gagatcgatg
     4141 actccctcga aagaactctc ggcaccgata gggatattca aaggcaccgg atgtgctcct
     4201 agcttgcttt tcatatcttg tagtacggct tcgaaattcg caccaaccct gtccatctta
     4261 ttgatatatg ctatgcgtgg aacgcgatat cgatcggctt gatgccagac tgtttcggac
     4321 tgtggctcta cgcctccaac agcgcagaat accgctatag ctccatcgag cacacgaaga
     4381 gatcgctcga cttcagcggt aaagtccaca tggcctggcg tgtctatgat gtttatttga
     4441 tgatcgcgcc aaaaggtggt tgtagccgcg ctctggattg taataccacg ctcctgttcc
     4501 tggtccatcc aatccatcgt agcttcgcca tcgtcgactt cgcctatctt atatgtcctt
     4561 ccagaataat agagcatgcg ttcggtagtt gttgttttgc cagcatcgat atgtgccatt
     4621 atgccaatat tgcgtatttt ccccaaatcc atggattact ccggagcaaa tataagcatt
     4681 gtaatttctt agaacttccg caagaccgga agacaagaag aaaagcccta aaaaccaggc
     4741 tataagaata tataggaaaa gcgaataaaa tacaacatca ggatagagcc ttataggact
     4801 gtcactccgt agcatttgtt attactggca tctttttggc gtttggatct gttttcttac
     4861 agccgcacat ctcatctcct tgcaccaaag tgccattaaa gcttataagg atactgtgaa
     4921 gaaagttgaa ttacttgcgc cggctggatc accggaagcc ctcgacgcgg ctattggcga
     4981 aggagcagat gcggtctatc ttggtttgcg ctccttcaat gcccgtatgc ggacaacgaa
     5041 ttttgccttt aatcaattcg aggctgcggt atatgcatgc cacaataact cgcgacgggt
     5101 atatgtaacc gtcaataccg tattcgaaga gcgcgaatcg gatcgcatct accagttgct
     5161 agaatatctc gcttccgtag gaccagatgg cataattgtt caggatgcgg gtgtcgcaaa
     5221 gattgcgcat gatcattttc cgtccttgcc tcttcatgct tctacccaga tgaatatcgc
     5281 ttcggtcgcg ggctgcaatc agctttcgcg agcgggcttt aagagagccg tacttgcccg
     5341 cgagctttca ctagaagaaa ttcggcagat aaggcagtat acaagccttg agctcgaggt
     5401 ctttgtccat ggtgcgctct gcattagcgc ttcaggactc tgcctgtttt cgagctatct
     5461 tggaggcaag agcgcgaata gaggccaatg tacacaggca tgcagaaggc tctacgaaag
     5521 cgaaacaggg aagggctact atttttctcc agacgatctt gaactcatag aatatattcc
     5581 agatctcatc atggcgggag tggatgcctt taagatcgaa ggcagaatga aatctgccga
     5641 atatgttgga gccgtggtat cggcataccg cttaatgttg gacaattacg agaaagatgg
     5701 cgaaaaagcc atgctaaagg ccaaggctat acttcagtct gattttgctc gcagaaaaac
     5761 gagttttttt atatctggca agcccgagga ctacattcac cctgagcaat ctggaggtac
     5821 aggcattgcc cttggaagaa ttcgcgatgc cagaacaatc gatggcaagc gctgggcctt
     5881 aatgaacagc cgcgaaggcc tggctgaacg agattccgta cgtatccata gaaaagacga
     5941 ctcaggccgc ataaccacca agatcctggc gatacgatac gaagtcaatg gaatgcttct
     6001 tcttttggat ggagactggc gtcaaaacga tgagctatac ctaatccaaa cagccagcat
     6061 gacgcggagc cataagccgg tgctacctaa gaatctcaat aggtttcata aatttccaag
     6121 caaccacaca gccccgcgcc ccgcaatttc gcgtctcgat ggcagaaaac tcgagatttc
     6181 gttcgaaccg ggaaactata tcctggtagg caaggtagct catctgcacg ccgctctgac
     6241 ctttcgccca aaaaaggcca tgatactttt caacaaactg aatgcagaga caatgcgcct
     6301 ggaagagaaa agccttcctt ttaagcgaga taggctcatc ctctggctcg atccatatct
     6361 tgccgaatcc gattccacat ggcttagcgc cgaactcgat tattggcttg aaaaaggtgt
     6421 ctcacttgta atcctaaata atcaagccca tttttcgatg ctgaggggga aaaaggtcat
     6481 ttcaattgct ggtccctggc tttacacttt caataaatgg gcacttgcct attatatcga
     6541 acagggagtg caagccttta tcccgccata tgaaatttct cgacaagatc tgtacagact
     6601 cacggaatac ctcccggctc agttctttgc tcccatagtt tttgcatatc cagatctatt
     6661 tagaattaga gcggacttat cgaggaaata tgacaacgca aaattcatgg atcgtgaaga
     6721 caactctttc agactcatag gaaaaagaga ttattctgta gttgttccag atagaccttt
     6781 ttctcttacc gatttggtac caaatcttaa gaagcaggga ttcaggcgct tcattgtaga
     6841 tctatcgaat gcggagccat ccaaaggcct atatagggat atcgcacgag ccatggaaca
     6901 aggtagtccg ctacccaata ctacgcgctt caactggaaa gatggtttct ggagcgaaga
     6961 aagaatgtac taagagccca tattaaaaaa gagctttcat aaataggtgc aatgtgggtc
     7021 ttaatgctct tgcctattcc ttgacgacat atccgtcagc gtataaagtt acaaaataaa
     7081 ctgctttttc atatgtgggc gataaattga actggattgg cattttaggg gtggcaaggc
     7141 atgagtgagt cggctggtaa ggcctttgat cttttatttt atctcgcaaa ggcaggagag
     7201 acagtctcgt tagctcgact ctcaagagaa actggtatga ataaggccac agcactgcgc
     7261 tatttgaatg tcctggaatc gaaaggtgtt gtggagcgca gcaaactggg ttggactctt
     7321 gggctttcgc ttttcgaact gggcagcaag gtaccggtgc gtcagctggt agtggataaa
     7381 gtacgcccta tcatagaaag gctcgctcga gagaccggcg aatccgtgaa cctggcttat
     7441 cttgccgatg aaacggccat cttcctcgat cgtgccgaag caaatcgaag tcttcgtatg
     7501 cgatcaatgc ccggagacag gctgcctctc tactgtacag gagtgggcaa ggcgatcctt
     7561 tctcagctca gcgaagatag gatggctgcc atcctgggtc catatcccct ggaaaaatat
     7621 accgaattca cctgtacaga tcctgatgac ataatcaagg aagtcagacg cgccagagaa
     7681 ttgggctatg gtatcgacag tgaagaattc gaggttgggc tcacatgtta tgcaatgccc
     7741 ctgcgtatcc ccagttcgga cttcgttggt gccataagta tttctggtcc aactgctcgt
     7801 atgaagaatc cggaaatccg cgatcgcttc atatatcttc tggaacaagc gataaaagac
     7861 gctattgaat tgctatctcc tcattatcat ttggacattg tatcaaagaa agccaagaat
     7921 taaagtctat ttgatatttt aacagagagc accttttggt cgaccaagcc tggctcagcc
     7981 taaaggcgct ctctgtactg gccgaatcct agaataccgg tgtctctatt gcgtctcgct
     8041 ttgagtcttc gataaaaggc gtttcggatt ttggggctcc ttcctgggct gccttgctca
     8101 taggtttgaa ttccacatgc tctgcgacaa tcttgactcg agagtgcgac tttccttcgg
     8161 tatccgtcca acgatcctgt tttagtcttc cgactactct tacaccgcgc ccttttttca
     8221 gattttgagc acaggctgaa ccaagtcttg cccatgcttc gatatcgaaa taggacactt
     8281 cgttttcata cttctgatct gcgattttgt aatttcgatt cgtggcaacc gtaaaatcac
     8341 agacttgatt tccggatggc gtcgatcgga aattgggatc gcggaccaaa ttaccttcca
     8401 ccagaatgct gtttaggtga ttcatacgac ctccttgcgt attagccgtg cgtctagatt
     8461 gcagctgcgt catcgctggc ctgcatgact aattcgccag gaatccctat ccctgattca
     8521 atttttatgc cctctttgct aatatataca catggaaaac tttgatcggt ttcaatctcc
     8581 ttttacgtgg cgctatggaa gcgaaaaaat gcgagagata tggagcgagc gcaataagcg
     8641 caaactctgg cgtcatatat ggatagctct tgccagggca gaatcgcgtt atggccttgt
     8701 aacgccttct caaattgccg aactggagag ccatgcagat gatatcgatt tcgaagaggc
     8761 caatagaatt gaagattcga ttcaccacga cctaatggcg gagcttagag tttttgcttc
     8821 gcaatgtctg gaggcaggcg gagtgctcca tctgggagcg acttcgatgg acatagagga
     8881 caatgcagat gtcctgagga ttcgcgatag cctttctctt gtaaaagaag attttacaaa
     8941 gctcctcctg gcgttggccg gaaaaataga agactatgcc tcattgccag ccatggcttt
     9001 tactcacttg cagcctgcag aacctacgac aatcggatat cgctatgctt gttatgctca
     9061 ggacctcgca gaggcctacg agcgcctttc ttatgcgatt gcggatatca ggggaaaggg
     9121 tttcaagggt gcggtgggca cttcggcatc ctatgcggag cttatcggat tggaaaacct
     9181 cgatgatttc gaaagagttt tgtccgagga tctgggcatt gaatttttcg atgtcagtac
     9241 tcagacctat gcgcgagttc aggattaccg gataatttcc ctattggcag agatcgctgc
     9301 gattcttcat aagatggctt ttgattttcg cattttgcag atgccgatgc tgggtgaact
     9361 ctgcgagcct ttcggagaac atcagattgg ctcttccgct atgcccttta agcgcaaccc
     9421 aatcgaatgc gaaaggatag aatctctggc tcgactcttt ccaagctttg cgaatattgc
     9481 atgggacaat gcggctttgt ctattctgga gcgtactctc gacgattcgg ccaataggcg
     9541 catcattcta cccgaagcat ttcttgccgt agatgagctg ttttcgacta tgctgcatat
     9601 tatagatggg cagataatcg ataatgaagc catccgatgc actctggatg cttatgcgcc
     9661 tttttccgcg accgagcggg ttctcatggc tcttggcaaa cggggcgcag atcggcagat
     9721 tgctcatgag cgccttcgta atatggcaat gctggcatgg caagcagtaa gagaaggaaa
     9781 ctccaatcct ctcaagtctc taatcgagtc tgatgaattt atcgcttcat tcttatcttc
     9841 agatactatc gaaaaactat ttgatgtcga tatttatata ggtgcagccg aaaaacgagc
     9901 gctggccatg gcagggcgca tccgaaggct cgcaatggat gaacattgaa ttgagcgata
     9961 tatttttaga tgctgatatt tttctcaagg agtagacatg ttagatttca tcagcaaggg
    10021 tggacctatc ctatgggtca ttatgggatt atctttggta gcactcgcga ttgtcataga
    10081 acgtctattg tatttaaagc ggatttcgat cgatgaagag aagctattct tacgcataaa
    10141 gacaagcttg atggaaggac atttttcgga agcgcttgcc atatgcgacc aaaatatatc
    10201 gcctttttcg aaattgctaa aggttggaat cgaaaacagg gaaaaacccg aatacttgca
    10261 gcgcgagctt ttgaaagatg ccgcatctct ggaatcggtc agtttggaga gagggcttac
    10321 tacgctcggc acaatctcca acatttcaac cttgcttggg cttttgggaa ctgtcacggg
    10381 taccatgcgc gcctttggcg ttttaggaaa atttggcgct gtcagcgatc ctgccgcgct
    10441 ggcaagcgga gtagcggaag ctctcattac aacggtaggt gggcttgtgg tcgcgattcc
    10501 tgtcatcatg atttacaatt attttgtatc gagaattaac ttgatcatga ctcgcctcga
    10561 aacgcaggtg aataatctgg tgagctttat ctcgagctat ggcggcaaag aacccgaaag
    10621 ggccgaggag aagccctcga aggaggcatg atggctcgga accacaaact tacgccgaag
    10681 atcaatttcg atcttacgcc tctaatcgat atcatacttc agctggtcat ttttttcatg
    10741 attactacga ccttcaggac tgccccgggc ataaacttgc agctcccttc ttccaagacg
    10801 gctcagacaa tctccacgcc cgaattgagg gtagtagtcg tgtcctcgga tgaaatctat
    10861 gtcgataaga ctaaaaccaa cattggaagc ctccaatcgg tgctgagtaa gcgcgtgagc
    10921 ggctcggatg ccaaacagat tcgcgcggtc ctagaaggtc gagccggggc ggaatatcag
    10981 ctgattatat ctgtgctcga tgcattgcga atgaatggcg ttgagaatgt cggccttata
    11041 acgcgaaaag aaaaggtggt gagatgagcg aggccctcac ctacgaagag cgagaacagc
    11101 gaaaacgcac tattcactca tatatcattg caattgcaat atatgctctt ctgttaggcg
    11161 tcggcatact attcgatatt cttaggccgg agagcattga tttcagcaat aaatccctca
    11221 ttgtgaatat tcaaggtcca gtaacaaacg acattggtaa ggggtctccg atcgaaaagg
    11281 aagaaagtct tgttgcggaa aaaccagcac cgcctccggt ccaagttccc aaacctcaag
    11341 cagataccag tgctaagcct aagccggcag agcctcttgt acccagtccc tctgtcgagg
    11401 ctgagcctac accgactccc attgttccgg agacagcagc agtacaagag ccggaaatac
    11461 cctggatccc tggcgaacga gggcctggtt cgcgggtcag ctcgagtgaa tcttcggtgc
    11521 tggcaccggg cgagggacag gtcccatggg gcactggaga ggcagtgcgc atcaccaagg
    11581 ctgaaaaagg aaacaccgtt gaaacgactc tgggcggatc atcggatagc gttggccaaa
    11641 gcctttatgt tcctatctat ttgagcatgc ctctgccgga gatcgttccg gccgagctat
    11701 tcaacgcaat accccctgaa attgttcctc caaatacact tattgcttct gccgaatcgc
    11761 gaaagcgagc ctttctcaac tattattcat ggaatggaaa ggagtatcgt ctgacaaatc
    11821 ccgtgccatt agaaatccgc gacaaactct gggagatgtt agaggatgca ggctataatg
    11881 tcgaaaaagc cgattataag gctggtcgtt tgctcaatcc ggttgtcata ggcttttcga
    11941 ttaccaagga tcgtcagctg agaggggttg aaattctgca atcctcggga gatccggaaa
    12001 tcgacgctgc tgtgctctat ggtttcaaac gggcgtcatt ctggaataag agtggggata
    12061 ccattcaggg gcgctttgtt tatagattct gatgtatact acccatacct atgatgtgtt
    12121 ataatacccg ccaataatcc atagcattat catcgggcag gagcaggtcg atgattatca
    12181 atctttcatc gctcattgct gctttttcat tctgcatcta tgtctttttc ataatttttg
    12241 gtatttcaag cagaaaagag cgcgtaagta tttcatttct tatatatatg gtcatgatgg
    12301 cactctggag tctcggcgcc ttcatggctt atagtaatat tggatttata agcctatccg
    12361 acgcaatcaa aataaagtta gtagggttac ttggggcacc gttcgccata tttgccacac
    12421 ttctctattt ttccaagagt cagaaaaagc tcttccgaag ttttctctat attggatatg
    12481 gcctatatgc ttatctgctc tatctgaatt tttccggctc cattgttgag aatatctggt
    12541 tcgaaggcct tgattatcat tatacccttg ggcaagatgc gctcatagtc tattctcttg
    12601 catatgttta cttgcttctg gctattcttc ttttgctgaa ggaacaaaag gtcaataaga
    12661 ataagttcca aaaacgcgct ctgcggctga tgctggtagg cgtagttgtg cttatggtcg
    12721 gagtattggc ttacctatac gaacccgttg ggcgctatcc agtggacatg attgcagcga
    12781 tgatcaactc gtcgataata ttttttacta tatataaata taaacttatt cactattctt
    12841 ccacagtatt caatattatt ctcggctcac tcgtggcagc cttttcgagt atcatatata
    12901 tgctgttctt tattttcctc ttcaagctcg acctctatgt gccaaactcc actttaatcc
    12961 ttctagctct ctgtcttggt atctgctctg ctctgatatt tggccccctg agaacagcta
    13021 tgcagactct tcttgaacat atctcgggtg gaaggagctt tctctattat agggaactca
    13081 ggaatttttc tactaagctc acttctattg taaagctcga ggagctttcg aagttgattg
    13141 tagataatgt gatatcagca ttaaagctcg agtgggcgtt tgttctctta aacgaatata
    13201 gatcgcagaa ttttagagtt atcgcttctc atggcttgga tttcatacag gaacaaggaa
    13261 accagaaccc gagtaatcag gtccttgtgc ccaagtcatc tcctttcgtc caggcctatc
    13321 aaaatcgtgc ctctcaagaa gaacaagtgt attcccagtg gaacataggt ataatgttga
    13381 taaaaggcca aaagcatcag acagtcctgg cgagccttgt acttccctta aagttcaagg
    13441 agaggctcaa tggatttatc attcttggtc cgcgcctgga gaaggattat tacaataagt
    13501 atgaactcga tgtattgaaa tttctcgccg atcaagcctc ggtggcaatg gaaaatgcca
    13561 taacttacga gcgcttgcgc cagcatcaaa agcgtcttca agatgctaac aaacagctga
    13621 taaaccagag aaagttcgaa gcctttttcg atggaatcgc aacacctatt tcgatacagg
    13681 acatcaatta caatatcgta accgccaact ttgccgctca gcgctatttc gaaaagactc
    13741 agaaggagct tataggatct aagtgctaca aggcttattt caatagggat cgcccttgtg
    13801 ccgaatgcct agctcaggac tgccttcata caaagctgcc tttcaatggc gaaagacagg
    13861 acaaccgaac ccaactgacc ttctcgctca atttctaccc cattcctacg caggaagcat
    13921 ctttaggggc tttcatcgag cttttccagg atataagcaa acagaaaagt gagcaggaag
    13981 acaccatttc agaagaaaaa ctcgctgcga ttggctcgct cgtttccaca atcgcgcacg
    14041 aaataaacaa tccaattggc ggtattcttg gcacaacgga tttgattctg gaagaggtta
    14101 cggagggcag cacaatccgt ggatatactg aggatatagc ccgttactct aagagcgctc
    14161 tggattttat caatagtctt atggtctatt caaagaagac acatacttct ctagagatgc
    14221 taaatatcat aaatgtgctc gaaaactctc tcaaaatggc aatgcggagt atcgattttg
    14281 gatctattat cgtgcgaaag tcctacgaca gcgtcaagga aattcttgca aattctaccg
    14341 agcttcagca ggtatttctt agcctaatca tcaatgctgt tcaggcaatg cgcagcgacg
    14401 gagttctgac gctttcctgt cggcaggaag gagatgatgt gctagtcttg atccaagata
    14461 ccggtatagg catgaggaat cagaaattcg ataggattgt caatgatttt ctgacggttg
    14521 aagaggcgag ctcggttagc ggcatgggct tgagttttgc taatcatatc gtaacaaaat
    14581 atgggggacg tatcttgctg agcagccagc ctggaaaggg caccactttt actgtaattc
    14641 tgccatccgc aatccaagac aaggacagaa tatggtttat caatgccagc gagccgaacc
    14701 aatttgatga tagctattat atacagcgca aggtgctcgt gggagaaaaa ggctacaagg
    14761 aagaaaccat acatcgcaag tgcgacgaga cggcttttca tatccttgct tataaagggc
    14821 ttcagcccat cggtaccgta tctattcatc tgagcgaagc aactgggcat attcctatcg
    14881 aggagaactt cgaaatcggc ccctatctcg atggcgctcc atatgcggag atcgacaggc
    14941 tagcggtgac gaaggaggag cgccagggct ttacttcatt cgaaatcatg gtgctatctt
    15001 atctatatgt tcgcggtcgg ggcgcaaaac agatttttat agataccttt gccgacgaaa
    15061 caggtctgat tcatatgttc aagaaacttg gcttcgagat aattggaggt tacagcaatc
    15121 ctcttccatg cactgtcttg atgatgaatc atgcttcgac atatgagcag gaaacgacca
    15181 agaatcggct ctttatcaag aaaactctct ctcgtcttgt gcctaaaatc gacttttccg
    15241 cagatgagat gtcatatgta atgaaagcaa tagacgagat aagcgccata tctccaggca
    15301 aggatagtgt agacactaaa gagatatcgt catacaatct attatgatat gaagcccgaa
    15361 gatttagcag agggactaaa caccatttta gctcactcac cggttttccc ggataagtgc
    15421 ctcattatcg atgtggttgc taatcccaaa gctggtggtt ttagccggat ccaccattca
    15481 aagaagcgaa tgagagagct aaaagagatg gtgagccggt cactcgagct gcctctacgc
    15541 aaggctccgt taagcctgaa aatgcacctg accgagagat gcggacacgc tgcaactata
    15601 gtccagcgca tactggagca ttctccatcc aatggaaaag attcctacca cattatcata
    15661 acagccggag gagatggcac aagtctcgag actgctgaac aactcacatt gcttccggaa
    15721 tccgagaagg atcgctttgg attagttaga ttgccctttg gaactggcaa tgacggctcg
    15781 gagggaagag atctctttgt tgctctagag aggttcctca atccagcaat tctcgagcga
    15841 aagccggcgc taaaaatcac cccaagctat gaaggaggag cgctgcctcg ctattctttc
    15901 aatatcgcca gcataggtct ggatgcctat gtggcggaga tgacaaatcg ccttaagcgt
    15961 cattttcctg gggattccta caagttttgg gtcgatatag ggactatgtt ctatgatcgt
    16021 gtctataaag tggttaacat gggtctcagg gcttggaaca gaggcagact tgtactggat
    16081 tccgagcttc ctcgactctt ggtagccatg ggagcttcgg gcaaccgata ttatggctca
    16141 cataagagca tacttcccaa tgagcacaac tgcattgcca tatctcagac cagcctcttt
    16201 agaaagctca ttttaaaagg gtccatagaa aatgggaagc acgaacatat ccgagaactt
    16261 acacatttta cagcggacag actccagata gactaccacg agagcattcc cttgcagtgc
    16321 gacggagaga cgactctcct tgcaaagtgt gactttccgc ttgaaatgga gagacttcag
    16381 aacgcctaca atgtgcttgt gtcggtgtaa agcgcaaaac gccttatttt ggaatagatt
    16441 gcgccaacag gactgagggt cgactgcaga agctcaaaag aatcgatata taggctcagc
    16501 attgggggtt tagggagcga cgaaagattg ggggcataag gcagcttgag ccgcgcgagg
    16561 gtaagatgag ccttccatgg gcgagcatcg gtctgtgctc caagttgggc tgctatctcg
    16621 cgtatcctat ctattaaaat cgtaagcggt tctgatggct ctatacgcgc tgcaagatag
    16681 agcactcttg gtctattcat ggctgggaga acagacagcc cttctgtagt tatcctgagg
    16741 ggagggcaga gattagccgc atcggaaagc aaattttcaa gctctttcgc cttttctact
    16801 tctaattctc caaagaaatg aagagtcaga tgcagattct gagggggaac ccaagaagga
    16861 cgggcaggtc taaaagccgt ctctatagag gcgcagtagg ttcctatctg attctggaca
    16921 gcagccggaa aaggcagagc cacgaagaga cgcatggcct tattatgtca gataatcctt
    16981 ccttatgcga gacatagact caaaaatatt ccgcttcttt tggctcgagc cctcggtcaa
    17041 atgttcgagg agttctcttg ctttttttcg ttctccttct aaattgaatg agcatgtata
    17101 tgtgtaatcc gacaaaccca attcatcggc acaagctaaa acttgctttt cttcacacag
    17161 agcaaggggc cggatgactg ttatggggta ttttctatag ggcatgacag gcggcatggt
    17221 tgcaaattcg ccttttctca gcatgttcat gagcagagtc tcgagaatat cgtccatgtg
    17281 gtggccaaga gcgagcttat tgaacccatg ttccttggca aatttcatga gctctgatcg
    17341 gcgctgggtg gtacaccagt agcagttcat tgatcttcct ggttttagtc tgcccaccac
    17401 aggtatctca agacgcgtgt atggaatacc ccatttggtc atatacaagg caagccagga
    17461 atcgtcccgc ctctgcgaaa ggccaaaatc ctcaaggtcg gtgactatat gacaggcggc
    17521 aatttcgaaa ggagtttcca gccattggcg tttcttggac agatcccaag cgaggcaaca
    17581 ggaatctttt ccgccggaaa ccgcgattag gattctatct ccaggctgaa tcatatcata
    17641 gcggcgcaga gcaatctcgc aaagcttgga aagagtggca cgagcgtttt tcattttata
    17701 taaatgcaga tcaaggccgc tcaaaggtca tgccagccat agatgctgac tcgatttgca
    17761 atcggaatct ttttcttttg aagctgggtc tggctctttg tggccttcgg aggggccgat
    17821 gaccgaattg atgaaagaaa cgagcacgtc cagtgcccgc tcattgaatc ctccctcggg
    17881 aatgattatg tcggcgtaag ccttggttgg ttcgataaag tcgaagtgtc caggccttac
    17941 cactgtcaga tattgttcta ctacggaatc tacggtgcga ccacgctcct tgatgtctcg
    18001 cgataggcgt cgaataaagc ggatatcatc cggtgtatcg acaaagatct ttagatctat
    18061 gaggtctcgc acttctttgt tagtgaagat cattattccc tcgaaaatga caagcttcga
    18121 gggctgaata tggatagtct gatctgttct tctatgatga acgaaatcat agacaggcat
    18181 gtcgatgggt tggccattct ttagagcggt gagatgagag atcagaagct cgttgtcgaa
    18241 ggcatcagga tgatcgaagt tgaatgctgt gatgttgagg ttactgatga attctgctga
    18301 cttgtagtaa ttatcctgtg gcaggaagac aaagtcgctg acaatttcgg atatttttcg
    18361 aactatagtg gtcttgcctg agccggaacc accggaaata ccgattatct tgactgctgg
    18421 catatccttt tttaccatgc cttggcttta tggtaaagtg gaaggcctat ccgttctgcg
    18481 ccatccgaat aagcatttcc tgaatttcat cgataatgaa gtcgggagta gaggcacccg
    18541 cggtaattcc tatgcgatca taactcacca tctcgggctt cagttcatct gccgtctcta
    18601 tatgccagga aggaagaccg aagcctatgg cactcctata gagtctcttt gtattctcag
    18661 agttcttgcc tccaactaca agcagcgcgt ccacctgtgt gctgagttca gccaatgcct
    18721 gttggcgctc tcgcgtagct ggacatatag tcttttctgc gatcatcttt ctaacatagg
    18781 attgaaaaat atcgataact gtatcgtagt cttcctgacg gattgttgtc tgtgcaatga
    18841 gggcgcaagg cttatctctg agcgatctcg ccagggcagg ggctgtttcg gggctatcta
    18901 caacgagagc attcaaggta tgtcccagaa tacctgcaat ctcaccatgt cgggaatcgc
    18961 cgacaagcac tacttgccaa cccaatctct cgtaatgccg ggctagaccc tggctcttga
    19021 tgactcgggg acaagtagca tcgattattc ttgcgccaag cacatgtagg cgagatattg
    19081 cggccagcga tgctccatga gcccttatta tgacaatacc atctctcagg tttgggagtt
    19141 catctagttc cgactcgatt gtgcttggat ccagaacatg cacaccaagc gattcgaggt
    19201 cggcaactgc ctgaggatta tggataagag ggccatagct atagacaggg cggccggttc
    19261 gagcagctct ttcggcggct cgatgcgcca gctcgtccgc gcgtcgcacg cccatacaca
    19321 taccaagaat tcttgctcga acaatctgca taattggatc ttatcagatt ttgatagcca
    19381 tttccacaat gcgcattgcg gtttttttga aaagtgctat actactattt ctggaaaatt
    19441 tcgctatccg gaaaaggaga attcatggca atgcgcaaaa ctattgtcgt ggcgcttggt
    19501 ggtaatgcga taatcgaaga aggcacggaa ggaacgatag aacagcagtt tgaaaatacc
    19561 cgaaagagca tgaaggcaat agttggcatg atagccgaag gccacaaagt ggttctgaca
    19621 catggaaacg gccctcaggc tggtgtacat ctcatccgaa acgaagcagc ttccgagcag
    19681 gttcctccat ctccactaaa tgtcattgtt gctgatacgc agggttctat gggctatatg
    19741 attgcacaga gtctttccaa tgcactgcgc gccgtaggca tagacaagga tgtggtaacc
    19801 gtaataacgc aggtagtagt agacccaaat gatccctcaa tgctgaatcc aactaaattt
    19861 gtaggtcctt tctacaaatt cgaggaagta gaaaaactgc gctcccgcgg ctgggtgatc
    19921 aaagaagatc caatgcgcgg ttataggcgc gttgtgccaa gcccaaaacc actcgatgtc
    19981 atcgaaaagg agacaatcaa ggaccttata gacgatggca agatagtcat cgcggttggg
    20041 ggaggtggaa ttcctgtcaa gcgtaatccg gatgggaccc tctcaggcgt agatgcggtg
    20101 atcgataaag acagggcttc atcgctcctt gcaagtctta tagatgctga tgagctcatc
    20161 atacttactg gtgtagataa ggttgcaatc aatttccgaa agcccgacca gcgggtattc
    20221 gatcatctca gcctaagaga atgcgaacaa tacatggctg aaggtcattt cccgaaaggt
    20281 tccatggggc ccaagatcga agcagcctgc gactttataa ggagaggtgg cgcaaaagtc
    20341 attataactt ccatggaaaa cgcaacggcg gcagttgatg gcaaggcggg cactgtgatc
    20401 tccgcttagc gcaacttgac cattcgagct gctccatggt aattttgctg gaattctttt
    20461 tcgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20581 nnnnnnnnnn nnnnnnnnng tgtttgtgat cttttacttt ttgatcattc ggccacagaa
    20641 caaaaagcag aagaagatgc agcagatgat agctggagtc aaaaagggcg ataaggtcgt
    20701 aaccattggt ggtatccatg gtgtggtgac ttcggtcaga gattctactg ttttgattaa
    20761 ggtcgatgat ggagcgcgga ttgagttttc taaatcggcc atagcttcca tcagccccgc
    20821 caagactgag gaaagagccg aagacaagta cgacgaaaag atcgaagaca agccagagga
    20881 cagcgctgaa tcgacccgaa aagtcgagaa agaaaaaacc gaagacaagt aaagtcttct
    20941 ttgtgcaata cccagctttc gctcgggtag gcataacaca tttctctcca tttattgata
    21001 catccatgag aggattggag taaccggtat gagcaaattg cgccgcctca tgatcgtcat
    21061 tgcagtgata gcccttgcat ttatgttcct ttttccgagt attcagtggt atttcctgac
    21121 accgaaagag gatcaagcca tt
//
LOCUS       DAOOWY010000024        20730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2562_length_20730_cov_7.448319, whole genome shotgun sequence.
ACCESSION   DAOOWY010000024 DAOOWY010000000
VERSION     DAOOWY010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 20730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20730
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2562_length_20730_cov_7.448319"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1134
                     /locus_tag="PK105_03350"
     CDS             124..1134
                     /locus_tag="PK105_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOU60775.1"
                     /translation="MQNSRQIISLPYRSTAFWLSLAHEPVESCKRNELGVPLDPILAQ
                     AVPSTAESRASPWDRCDPLGEESHSIADRVIRHYRSRVLVRATGQCFLFCRHCYRRSL
                     LPSELEFIDESTIDKLILLLRSHPEIREVLISGGDPLIASDEKLERLLSAIRSVERPI
                     LIRLCTRAPIVLPKRISDNLISLLSELRPLQIMLHINHPRELSPEFLDKSESILKAGI
                     PLHSQTVLLRGINDNAETLIELFSILNLHSIHPYYLFQGDLAIGTAHFRVPLSQGLII
                     YEKLKRELSGLELPRYSVDAPGGGGKVYLPEGIVERKNDSWVLRLPDGSLCEYPEEHL
                     QC"
     gene            complement(1169..2743)
                     /gene="guaA"
                     /locus_tag="PK105_03355"
     CDS             complement(1169..2743)
                     /gene="guaA"
                     /locus_tag="PK105_03355"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOU60776.1"
                     /translation="MDKIIILDFGSQYTQLIGRRIRDLGVYSEILPGEKALESAASKG
                     CLGFILSGSPFSAMQKEAPALHPSILESGLPILAICYGIQWLTLQSGGIVERSKAHEY
                     GPMTVSFLPKARQGGNPWSLFSEGLPERFLSWMSHGDSIVLPGDGWEIIGVSESGVPA
                     ILAHKSKPWFGLQFHPEVSHCEHGMDILSRFVFGVCKAKATWSLETYLDEESKTIETR
                     VKSEPVLLLISGGVDSTVTGAFLLRCLNPEQVHLLYIDTGLMRKAENEEVMLNLSRLG
                     ARHLETVDAESRFLEALKGVLDPEQKRRIIGDMFIRVQEEAVQRLGIPNAFLAQGTLY
                     TDLIESGKGIGDKAQVIKSHHNVRSPLIEKKRAEGRVIEPLDKLYKDEVRALGRALGL
                     PEEIVGRHPFPGPGLGVRILGEVTKEKCDILREADSIYIEELRKRGLHEQIWQAFCVL
                     LPVRSVGVMGDERVYRYVVALRAVRSEDGMTAQPFAFEMKDLFEISACITNQVPQIGR
                     VVYDISSKPPATIEWE"
     gene            complement(2731..3957)
                     /locus_tag="PK105_03360"
     CDS             complement(2731..3957)
                     /locus_tag="PK105_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOU60777.1"
                     /translation="MNVVIIGAQWGDEGKGKIVDFLAADAQIVVRFSGGANAGHTIVL
                     GGERFALHLIPSGILYSDKTVILGSGMVIDPEALFGELSDLENKGIDWKGRVFISDRA
                     HLVLPGYREMDREADARRAKPIGTTGKGIGIAYALKASRDGIRIADIDNIERLVNLRL
                     EDRKWLLQWKDKLIALRIDLAGYLRSHRNSWRLFEGAQGALLDIDTGTYPFVSSGMSC
                     AAGAAAQGGIGPRAIDRVLGVFKAYSTRVGNGPFPTEFADDSPGSLSQYIRKVGNEYG
                     TTTGRPRRCGYLDLVALRYACAVNSIDRLVLTHLDVYDALEEFEACIGYHIDGRLTED
                     FPASIRDLEVAQPVLRTFKGWKRSIGNTRTYGDLPLNAREYIEFIEEFTETPISIISV
                     GSDRNQTIVRESPWIR"
     gene            complement(3974..5575)
                     /locus_tag="PK105_03365"
     CDS             complement(3974..5575)
                     /locus_tag="PK105_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOU60778.1"
                     /translation="MANNSPSIALVLNAHLPFVRKPQYRQFYEERWLFEVISETYLPL
                     LRMFRKLELENIPFRVNMVLSPTLLAMLSDELLCERYIEYLDNQIELAQKERRRLAGD
                     PVFEPLADLYFAMYSRSREEFEDLYGRNIIRAFDYFSKKGYIELMTTAATHAFLPTYS
                     DIPEAIDAQIESAIIAHRMEFGKNPSGFWLPQLGWYPGLEKHLRAYNLKYTIVTTKGA
                     LLGSPSPFYGSFSPVMTASHIAAFIRDAGATKAIWSETEGYPAHSVYRDFYRDIGYDL
                     DPSYLAPHLYGVERGYTGFKYWAVTGKTDNKRPYEPSAASAQAIMHAHAYLSDRKVQA
                     RAASYWMKDRSPIIVCPYDAELFGHWWFEGIQFLEAFFRTMSRKDEENPLRLVTMSEY
                     LSENPSCPRSEPEFSSWAEGGYAEAWLDGRNDWVLRHTRRAAERMRELTIRFPNESGL
                     RERILNQAAREVLLAMSSDWALLLRSGKSADLAARQIRESIYNFSHIYEMLSAHTVET
                     EWLTSLEKRNNIFPYMNYHVFAPKK"
     gene            complement(5575..6261)
                     /locus_tag="PK105_03370"
     CDS             complement(5575..6261)
                     /locus_tag="PK105_03370"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOU60779.1"
                     /translation="MNPEILSLLSDEELRTLAEKMNIYVPEGLERIFLIEEIIDAYED
                     DTSDKVYSHDAPDHLEEKKLAGTGFIPGRLEDISIPDYYNETYINAIVRDPLWIFAFW
                     DIADSTRQKYIGEAETSRLLLRISEATGFEPSFSYDIVVSFDDRKWYINVPHPKRSYM
                     IDLCIAKCHKVRILARSNIVRMPSQYLDISSKLPQVTRSLLVLSGSEELHLIEPKEEN
                     SLRIMSMEGE"
     gene            complement(6308..8071)
                     /locus_tag="PK105_03375"
     CDS             complement(6308..8071)
                     /locus_tag="PK105_03375"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOU60780.1"
                     /translation="MSVVRTTPVPPDVDIAQSADIKPISDIASSYGLEEKYLEHYGTD
                     KAKVRLEFLSDRQGQSRRGKYIDVTAITPTPLGEGKTTTTIGLAQGLGYLGKKSIATI
                     RQPSMGPTFGIKGGAAGGGYSQIVPMTDFNLHLTGDTHAVSAAHNLCAAAIDARIYHE
                     SRWSDSYFEKLGLKKLNIDPYSVLWRRVVDMNDRTLRSIIVGLGGLENGPLRETGFDI
                     SVASEVMAVLALATSLDDLRRRIGKIVVALDKSGKAVTTEDIGVAGAMTILLKDALKP
                     NALQTLEEQLAFVHAGPFANIAHGNSSITADLIASEISDFVVTESGFGSDMGFEKLMD
                     IKCRSSGLMPDAVVLVATVRALKMHGGGPKVTPGKPLSSAYTQENLELLRAGLSNLIA
                     HIEIIGKYGLPVVVSINAFPSDTKAEHELIRDAALKAGAFDAVISRGWSLGGEGCAEL
                     ALAVDKASSQPHKANLLYPLDMPIKDKIEIIAHEIYGAGTVVYTPEADAAIGKYTDLG
                     YGNFPICMAKTQYSLSHDPAVKGVPRDFDFPVREVRLSAGAGFIVPITGEISTMPGLP
                     SKPAFIGMDIDTMTGRITGLS"
     gene            complement(8301..9281)
                     /locus_tag="PK105_03380"
     CDS             complement(8301..9281)
                     /locus_tag="PK105_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOU60781.1"
                     /translation="MRDSITDVPGIRVGNAQDQDKARGCTVVLCENAKTAGVDVRGGS
                     PGTRETDLLNPVNMVQAPHAIYLSGGSAFGLAGADGVMQYLSENGIGFHLLNLVVPIV
                     SGAVIFDLTVGDPSAYPGPAMGREACMNASQEVSMGNVGAGTGALIGWAGNLNQRMKG
                     GLGTASASRGNLVVGAIVVVNCMGNVYDPETGELLGGTLDPTGSYILDGYEEMKNKQD
                     ALSFQANPEGLTTHTTIGVVATNAILSKPMATRMAMMAHDGYARSINPSHTSGDGDTI
                     FSIATGEVESNIDIVGSMAAEAMARAIAKAIRYADSAYGYKSYSELSKSL"
     gene            complement(9365..10180)
                     /locus_tag="PK105_03385"
     CDS             complement(9365..10180)
                     /locus_tag="PK105_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HOU60782.1"
                     /translation="MIAGIDIGSTSTKVVLLDNGCILCKMRVLASDAVTAAAGALGKL
                     DTEKGYKIQSIDRIAITGAGASKIHQDIFDIPTVKIEEIMAIGHGGKFLSKRDDIIIA
                     NIGTGTPFIEVNQNGIAHIGGTGVGGGTIIGLAKALIKTSDFNTIMDLASKGDLSRVD
                     LMIKDIADTDISFLAKDMTASNFAKMLESATREDIAMAIINMVYQVIGMLSVFVAQSR
                     NKDSIIVTGNGNNNAIGRNVLGSISEMYGIHFEFPEDAEFSTAIGAVLAASSA"
     gene            complement(10250..11074)
                     /locus_tag="PK105_03390"
     CDS             complement(10250..11074)
                     /locus_tag="PK105_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60783.1"
                     /translation="MFGKSQKILFDLAVLYLLILMISAIAAPLIAPYNPTEQFLLKRF
                     HSPSLENIMGTDNFGRDILSRIIYGSRSALIVGIVTVAISLAIGMVIGLLAGMGTRSL
                     DNVLMLFMDSLLSFPTILLAITVVSFLGYGIVQVMLALGIISSPIFARLIRAETLAIK
                     TEGYIEAARALGTSSFKIAFKHIIPNLLGKVAVQCSLTFGQAIVIESSLSYLGVGTQP
                     PDASWGLMLKDARNYLIQAPWMAIYPGLCLALTVLAFNIIGDSLAESFNPRLTGSS"
     gene            complement(11081..12580)
                     /locus_tag="PK105_03395"
     CDS             complement(11081..12580)
                     /locus_tag="PK105_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU60784.1"
                     /translation="MKRSVILGMVFLALLSGVGSSLLAQGKGSIAFGLSGNPDTLDPQ
                     KTAGTLTFQVVKSFYDTLVEPDMTGKLVPALAESWTVSPDGLTWTFKLRPGVVFHNGQ
                     ALTSRDVKATLDRIRDPSFASPRRSEFTAIKEVKTPDPQTAVIILGAPYAPILANLAS
                     GWGAILPADLIQSGHNFDSEPVGTGPFKFEKWIRDNKISMVRNEKYWMKGLPKLARVD
                     FQIVPERSVQIQGLMSGAIDAVEYVDPEDLPILQSNPNITIRKEQTSMILVMAMNCSR
                     EPLNDLRVRQAINYAVDKQLVLDIAYGGGKVGSSFMDTGNAYYTDFSHFYPYNPDKAK
                     QLLKDAGVGNRQFTITVPQNYPLHVKAGELIQQMLEKVGMNVKIQLVDWSSWLARIYT
                     EGNFDLTIIGHTGKLDPDGTLAGYGTDKKYVRWINPTCDARIKEAATISDFAVRKKLY
                     TEALEIMAKEVPFVYLGSSYRYVAMRSNIADFRITPNLDSYDFRWTELK"
     gene            complement(12604..13590)
                     /locus_tag="PK105_03400"
     CDS             complement(12604..13590)
                     /locus_tag="PK105_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU60785.1"
                     /translation="MADLIESKNITKIYRSRGLAGSKHGFKAVDEVSVNIKKQSIFGL
                     VGESGCGKTTFGRALMYLDPPTSGSVIFDDVPLGSLKPKELRRFRNRMQIVFQDPNSA
                     LDPKMSIYNSLSEGMVNLKIPKEQRDRKVNDLINLVGIGKAYLRRFPHELSGGQKQRI
                     VIARALSMDPDFLVLDEPTSNLDVSIQAQIINLLIDLKNEFELTYLFISHDLNLVSYM
                     CDEIAVMFKGRIVEQAETELLISEPSHPYTQKLFSSIPGGAQRIGVKSLNIGELGEKT
                     IREPQKEEKSQAGCSYYSQCPMGDAECSLSAPFLRELSRGHVVSCHKIRTGK"
     gene            complement(13575..14561)
                     /locus_tag="PK105_03405"
     CDS             complement(13575..14561)
                     /locus_tag="PK105_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60786.1"
                     /translation="MERMASLLSIEDLRVAFSLGKKETTVLRGINLSILGNEIHGLVG
                     ESGSGKTVTSTCIIGLLPIPPARILSGHIYFDGKDLLSLPEDQMRTYRGRKIAMIFQE
                     PAKYLNPAFKIGEQIIEMLMLHMGMKKRSAYERVVELLRLVGLDEKVIDSYPHELSGG
                     MKQRAMIAMAISCDPDLIIADEPTTALDVTLQVQILALLKKLKDMRKLGVLFISHDLY
                     LVSEIADRVSVIYAGRIVESATKEELFRNPLHPYTRLLMHSIPDAKKKGSKLATIPGQ
                     VPDAAEIGNGCAFANRCPIAKEDCFNDIPSYVAYENAHTVACYYAGKIWQIL"
     gene            complement(14562..15488)
                     /locus_tag="PK105_03410"
     CDS             complement(14562..15488)
                     /locus_tag="PK105_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60787.1"
                     /translation="MDLIRFIARRFLRLILSAFIIATIVFFVIRVIPGDPALIIAGID
                     ANPADVQAIRTRLGTDRPILVQYGDFIWHMVRFDFGNSMISGEPVLKLILERFPLTLK
                     LAIFGIAFSIVVALPLGVLSAVKRWSAWDYFGLVLSQLGMAIPSFWLGIILLLIFAIK
                     IPIFPLFGSETFAHYILPIVSLGFGRAAIILRHTRASMVEELSKEYIITARAKGLTER
                     MVKYKHALKNALLPIITVTSIELGYMLGGAIIIEQVFSMPGLGRLFLSGIYQRDFPII
                     QGGVVFVAILFSFINFIADILYSVANPKIRLS"
     gene            complement(15681..16898)
                     /gene="pcnB"
                     /locus_tag="PK105_03415"
     CDS             complement(15681..16898)
                     /gene="pcnB"
                     /locus_tag="PK105_03415"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOU60788.1"
                     /translation="MRYFRNSNGSLKAKARIYGQEDHCIDISCIDREALAIVNRLVSH
                     GYSAYIVGGAVRDLLLGRRPKDFDIVTDALPNRIRKIFSNSRIIGRRFKLVHVYSNRK
                     IYEVSTFRSLSNGSVGNEYGTISEDVLRRDFTVNALYLDPIALEILDFVGGVDDIHSG
                     KLKAIIPLDRIFSEDPVRMVRGIKYAATNQFVIPFSLRLAIRRDASLLDSVSPSRLAE
                     EFYKIIATGKSVEIFQALDKYGLLQYLSPEVHASIHGDKAFKEAFLRDLASMDMLAQV
                     PEGTSLLNSATPAEIIQTDKRLISPFLAWFIRQRIKDFSFLRDISKDPVTFRHQIFSD
                     IRVFLSPLNLPRVALEEAMEQVLQEENLLPAPPPPKRRKQGQRKKKAKPRDESMHLKV
                     SDIDYTNAYKPGF"
     gene            complement(16944..17858)
                     /locus_tag="PK105_03420"
     CDS             complement(16944..17858)
                     /locus_tag="PK105_03420"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU60789.1"
                     /translation="MEKFRASDGKELAYLRFDPKNEAKAVVIVGHGMNDHKERFMHLA
                     EALTPIGVSVWIPDLRGHGDTDSGTNRGYLADKDGFERVVQDLIEMGNYASSCMGELP
                     LFYFGHSFGALIGMALIATYGKYLHGAVLSSPPEKQKLFMDKIGGLMISMGGALKGFH
                     APAKLPNQMTFGQYAKSVTDAKTKFDWLSRDSAIVDAYIRDPKCNFICTYGFYRDLLH
                     GVRKVYSNGFLESIPPSLPLFLLCGSADPVIGMKEGYEKLGQRFKLLGMIDFESKCYD
                     GGRHESINEINKQEVINDIIDWFSRHIQ"
     gene            17947..18579
                     /gene="tsaE"
                     /locus_tag="PK105_03425"
     CDS             17947..18579
                     /gene="tsaE"
                     /locus_tag="PK105_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOU60790.1"
                     /translation="MSSLDLQLYASFSSGLEKTDFREYYRIREYAEGGQYLIILSSPY
                     VRRTIAIGRAIGRAAPGGSIVAFRGDLGAGKTTLARGVAQGLGIAEPIISPTYTIIAE
                     YQGRLHLVHIDAYRLSDEDEFVQTGGEELLGSPGSLSLIEWSERIAGVLPNETQYITM
                     VVKEGGNRIILIEGDWIENIDWQRFKIPYSIDLQLASTLSKRYQNDCPVD"
     gene            18560..19222
                     /gene="tsaB"
                     /locus_tag="PK105_03430"
     CDS             18560..19222
                     /gene="tsaB"
                     /locus_tag="PK105_03430"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOU60791.1"
                     /translation="MTVLSIDTSIPTLRVGVSKEKTLVISALEPPVTHAEAILPAVEQ
                     VLQKAGITINNIELLAIAGGPGSFTGLRIGIATAKGLSLGLGIPMVLVPTLDIYGWAW
                     RELEGIVVPVIDARKHRVYCARYSFGQRIGDWMDIELTQLIWKIHGEDKVNFVGPDAE
                     LAEAICLEREGWILHRPNLEKEIESLSVLGINIYLEKGGANDTAGPIYLREPEIGTPI
                     AR"
     gene            complement(19223..20347)
                     /locus_tag="PK105_03435"
     CDS             complement(19223..20347)
                     /locus_tag="PK105_03435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU60792.1"
                     /translation="MPVEISKVESRKDLKRFIDFPVRLYWDQPNYVPAPRFDELRTLS
                     KDKNAAFEFCEAEYWTAWKDEKIVGRIAGIINHRFIEKWGKAWGRFGWIDFIDDSEVS
                     AMLFKTVEEWAASKGMEALHGPLGFTDLDREGMLVEGFNERATLATIYNYPYYPQHLE
                     MLGYRKDVDWLQFQIFTPKEIPEKVQHVADLLAKRSGVRLFEWKDKRVIVEKFGKQLF
                     ALIDETYGDLYGTTPLSERQVEDYIKQYLGFVDPRFTKILVDKDERLIGFGIAMPSLS
                     EAFYRSRGRLFPTGWYHVLKALNNPKVLDLYLVAVRPEYQSRGVLALVMNELQRSAME
                     AGVEYAETNLELEDNIKVQSIWKDYPKRQHKRTRAYKKEI"
     gene            complement(20331..20567)
                     /locus_tag="PK105_03440"
     CDS             complement(20331..20567)
                     /locus_tag="PK105_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60793.1"
                     /translation="MEVFDGTLNGLGHRRAVDPAFSIADAENQLAHLYVLDGNDWLGR
                     GLLGDLISEATIAAYELFIEEWRTEKGGKDACRD"
BASE COUNT         5624 a         5087 c         4339 g         5680 t
ORIGIN      
        1 cggatttagt gcacttttaa gttttaaaca gtgcactttt aaattttaac taacagaaaa
       61 agccatattc tataccctca gcctcattgc tcattatctt tttagaaatt atattgacca
      121 gagatgcaga attctagaca gataatcagc ctgccctacc gctcgacggc tttctggctc
      181 agccttgctc atgagccggt ggaaagctgc aaaagaaatg aacttggagt acccctagat
      241 cccattcttg cacaagccgt gccatccacc gccgaatcgc gtgcttctcc ctgggataga
      301 tgcgacccac ttggagaaga aagtcattcg attgccgacc gtgttataag gcattatcgc
      361 tcccgcgtcc ttgtgcgggc gaccggtcaa tgctttctct tctgtcggca ttgctatagg
      421 cgttcgcttt tgccctcgga actagaattc atagatgagt ctacgatcga taagctgata
      481 ttgctccttc ggtcacatcc ggagatccgc gaagtactga tttcgggtgg agatccgcta
      541 attgcatccg atgagaagct tgagaggctt ctatccgcga tccgctcggt cgaaagacca
      601 atattgatta ggctatgcac aagggcgccc atcgtgctgc ctaagagaat aagcgataac
      661 ctcatttccc tgttgtcaga gctgcgccct cttcaaatta tgctccacat caatcacccg
      721 cgagaattga gccctgaatt cttggataaa tcggaatcta tcctcaaagc cggtataccg
      781 cttcatagtc agacggtcct tttgcgggga atcaatgaca atgcagagac tttgatcgag
      841 cttttctcta ttcttaacct acatagcatt catccctact atctctttca aggagatcta
      901 gccatcggta ccgcacattt ccgtgtaccg ctttctcaag ggttaatcat ctacgaaaag
      961 ctgaagagag aattatcagg tttggaactg ccgcgttact cggtggatgc accgggtgga
     1021 ggaggaaaag tatacctccc tgaaggaata gtcgagcgaa agaatgacag ctgggtgctt
     1081 cgcttacctg atggaagcct atgcgaatac ccagaagagc atttacagtg ttgagaagct
     1141 caaatctgag ggttaggatc tattatgctt actcccactc gatagttgca ggcggtttgg
     1201 aggatatgtc gtagacaact ctgccaatct gaggtacctg attcgtgata catgcggata
     1261 tctcgaaaag atctttcatt tcgaaggcaa atggttgagc agtcatgcca tcctcgctcc
     1321 tgacagcccg aagtgcaact acatacctat aaaccctctc atctcccatt acacctaccg
     1381 aacgaacagg aagaagcacg caaaaagctt gccatatctg ctcatgcagc cctcgctttc
     1441 taagttcttc gatatagata ctatccgctt ctcgcaggat gtcacatttc tcttttgtta
     1501 cttcacccag aatcctcaca ccaagccccg gaccaggaaa aggatgtcga cctacaattt
     1561 cttcaggcaa gcctaatgcc cttccaagag ctcgaacttc atccttgtat agcttatcca
     1621 aaggctcgat aactcgtcct tctgctctct tcttttcgat aagagggctg cgcacattgt
     1681 gatgactctt tattacctga gccttgtcgc cgatcccttt gcctgattcg atcaaatctg
     1741 tataaagcgt cccctgggcg agaaatgcat tgggaattcc aaggcgctga acagcttctt
     1801 cctgaactcg tatgaacata tcgccaatta tgcgcctctt ttgttcaggg tctaaaacac
     1861 ccttgagcgc ctcgagaaat cgagattccg catcgacagt ttccagatgt cgagctccaa
     1921 ggcggctcag atttaacatg acctcttcat tctccgcttt tcgcatgaga cctgtgtcta
     1981 tatataagag gtggacctgt tcaggattca agcatctcag caaaaaagct ccagtaaccg
     2041 tcgaatcgac gccccctgaa atcaagagca aaactggttc agattttact cttgtttcga
     2101 tagtctttga ttcctcatcg agataggttt ctaatgacca agtggcttta gctttgcaga
     2161 caccgaacac aaaacgagac aaaatgtcca ttccatgttc gcaatggctg acctcgggat
     2221 gaaattgcaa tccaaaccat ggcttggatt tatgagctag aatagcagga actccgcttt
     2281 ccgatacacc gatgatttcc cagccatctc cagggagcac aatgctgtct ccatggctca
     2341 tccagctcaa gaatcgctct ggtagccctt ccgagaagag ggaccaagga ttcccaccct
     2401 gtcgggcttt aggcaaaaag ctcacggtca tagggccata ttcatgcgcc ttggagcgct
     2461 ctactatgcc tcccgattgt aaagtaagcc attgaatacc atagcaaatt gcgagtatcg
     2521 gaaggcctga ttccagaata cttgggtgaa gagcaggagc ttctttttgc attgcgctga
     2581 aaggtgagcc agaaaggata aaacctagac aacccttgct tgcagccgat tccagtgctt
     2641 tctctccagg caagatctct gaataaacac ctaggtccct gatacggcga ccaattagct
     2701 gggtatactg gctaccaaaa tcgagaatta ttatcttatc catggacttt cccgaacaat
     2761 tgtctgattt ctatccgaac ctacagaaat tatcgaaatc ggagtctcgg tgaattcttc
     2821 tatgaattca atgtattcac gagcgttcaa tggcaaatcc ccataggtcc tggtgttacc
     2881 aatagatcgc ttccatcctt tgaatgttct caggacgggt tgggcgacct cgagatctct
     2941 tattgaagcc ggaaagtcct ccgtaagtct gccatcaata tgatatccta tgcatgcctc
     3001 gaattcttcc agagcgtcat agacatccag gtgagtgagc acaagcctgt cgatagagtt
     3061 tacggcacat gcgtagcgaa gggctacaag gtctaggtaa ccacagcgcc gcgggcgccc
     3121 cgtcgtagta ccatactcgt tccccacctt acgaatatac tgggacagcg agcctggcga
     3181 atcgtccgcg aactcggttg ggaaaggacc atttccaacc ctcgtggaat aagccttgaa
     3241 gacccccaat actcgatcga ttgctcgcgg accgattccc ccttgcgcag cagcgcccgc
     3301 ggcgcatgac atacctgaag agacaaatgg gtaggttcct gtatctatgt cgagcaatgc
     3361 gccttgagcc ccctcgaaaa gtcgccaact atttctgtga gaacgcaaat atccagccaa
     3421 atcaatcctt agagcgataa gtttatcttt ccattgaaga agccattttc tatcttctaa
     3481 acgcaaattg acgagtcttt caatgttgtc gatatcagcg atccggatgc catctctgct
     3541 tgccttaaga gcgtaagcaa ttccaatgcc ttttcctgtg gttcctatgg gttttgcacg
     3601 tcgagcatcg gcctctctat ccatttctct atatccgggt aaaaccagat gagctctgtc
     3661 ggaaataaat accctgccct tccagtcgat gcccttattc tccagatcgc ttagctctcc
     3721 aaaaagcgct tccggatcta tgaccatgcc agaaccgagg atgaccgttt tatcggagta
     3781 tagaatgcct gagggtatca gatgtagcgc aaaccgctca ccgcccagaa cgatagtatg
     3841 gccagcgtta gcgcctccag aaaagcgcac cactatctgt gcgtctgctg ccaaaaaatc
     3901 gacaatcttg ccttttcctt catcccccca ctgtgctcct atgatgacaa cattcacaat
     3961 agactcctta ttgctatttc ttaggtgcaa aaacgtggta attcatgtat ggaaatatat
     4021 tattgcgctt ttctagactt gtgagccatt ccgtttccac ggtatgcgct gaaagcattt
     4081 catagatatg gctgaaatta taaatcgact cgcgaatttg ccttgcggcc aaatctgctg
     4141 atttgccact tctcaataag agagcccaat ccgaagacat ggctagcagc acttcgcgag
     4201 ctgcttgatt gagaatgcgt tctctcagtc cactctcgtt cggaaagcga atagtcaact
     4261 ctctcatgcg ctcagcagct cttctggtat gccttaggac ccagtcattg cgcccatcca
     4321 gccaggcctc ggcatacccc ccttccgccc aggaagagaa ttcgggttct gatcgaggac
     4381 aggagggatt ctctgacaag tattcagaca tggttacaag gcgaagtggg ttttcttcat
     4441 ctttgcggct catggtgcga aagaaagcct ccagaaactg gataccctca aaccaccaat
     4501 ggccaaagag ctcagcatcg taggggcata ctatgatggg agatctgtcc ttcatccaat
     4561 aggaagcagc acgcgcctgc acttttctgt cggagaggta ggcatgcgca tgcattatgg
     4621 cctgagccga tgctgccgat ggctcatatg gacgcttatt gtctgtcttg cctgttactg
     4681 cccaatattt gaatccagta tatcctcgct caacaccata aaggtgggga gctagatagc
     4741 taggatcgag gtcgtaccct atatccctat aaaaatctct atagactgaa tgagctggat
     4801 agccttcggt ttcagaccag atcgctttgg ttgctccagc gtcgcgtata aaggcggcaa
     4861 tatgcgaagc agtcatgacc ggagaaaatg atccatagaa tggtgagggc gaaccaagca
     4921 atgccccctt ggtcgtcact attgtgtatt taagattata agccctgaga tgcttctcaa
     4981 gccccgggta ccatccaagt tgaggtaacc agaatcccga agggttcttt ccaaattcca
     5041 ttctatgggc aataatggca gattcaatct gagcatcgat ggcttcggga atatctgaat
     5101 aggtcggcag aaaggcatga gtcgcggcag tggtcatgag ctctatatag cctttcttgg
     5161 aaaaataatc gaaggctctt atgatattgc ggccatagag atcttcgaat tcttcacggc
     5221 tcctgctata cattgcaaaa tatagatcag ccagaggctc gaacacagga tcccccgcaa
     5281 gtcttcgcct ctccttttgg gcaagctcga tttgattgtc gagatattcg atgtatcgct
     5341 cacaaagaag ctcgtctgaa agcatggcta gcagagtagg agaaagaacc atattcactc
     5401 taaaaggaat gttctcgagc tccagtttgc gaaacatcct cagcaaagga agatatgtct
     5461 ctgaaataac ctcaaaaagc catctttctt catagaactg gcgatattgc ggcttgcgca
     5521 caaaaggaag atgagcattg agaacaagag ctatcgaagg ggaattgttg gccattattc
     5581 tccttccata ctcattatcc gcagagaatt ctcctcttta ggctcgatca gatgaagttc
     5641 ctcggaaccc gagagaacaa gaagacttct tgttacctgc ggaagtttgg aagatatatc
     5701 gaggtactgt gatggcatgc gcacaatatt ggaacgagct agtattctga ccttgtggca
     5761 ttttgcgatg cataggtcta tcatgtagga acgctttggg tggggaacgt ttatgtacca
     5821 cttgcgatca tcgaagctca ccacaatgtc ataggaaaaa cttggctcga aaccggtggc
     5881 ttcgcttatc cgcaagagaa gccgagacgt ctcagcttct ccgatgtact tctgtcgtgt
     5941 agaatcagca atatcccaga atgcgaaaat ccacaatgga tctcttacga ttgcgttgat
     6001 atatgtctcg ttgtaataat ccggaataga aatatcctct agccttccgg gaataaaacc
     6061 tgtacctgcg agtttctttt cctccagatg gtccggagca tcgtgagaat ataccttatc
     6121 cgacgtatca tcttcgtaag catcgatgat ttcctcgatc agaaaaatcc tctcgagtcc
     6181 ttctggaaca tagatattca tcttctcagc tagagtcctg agttcttcgt ccgaaagaag
     6241 tgacagtatt tccggattca caatagtata gtgaaagaat gatcatgtcc ttgtcaatat
     6301 gcatgtatca agacaaccca gtgattctcc ccgtcatggt atcaatatcc atgccaatga
     6361 acgctggttt cgatggcagt ccgggcatcg tacttatctc gcccgtaatc gggacaatga
     6421 aaccagctcc agctgaaagc cgaacttccc gcaccgggaa atcgaaatct cttggaactc
     6481 cctttacggc aggatcgtga gaaagggaat attgcgtctt tgccatgcat atcgggaaat
     6541 tgccatagcc aagatcggtg tactttccaa ttgccgcatc ggcttccggc gtgtatacaa
     6601 ctgttccggc tccataaatc tcatgagcta ttatctctat cttatctttt ataggcatat
     6661 cgagaggata aagcaaatta gccttatgcg gctgagatga agccttatcc accgctaaag
     6721 cgagctccgc acagccttct ccaccaagag accagcccct gctaattacc gcatcaaaag
     6781 cacccgcttt gagagccgca tccctgataa gctcatgttc agcttttgta tcgcttggga
     6841 aagcattgat agagacaact acaggaaggc catatttccc gatgatttcg atgtgagcga
     6901 tgagatttga caatcctgcg cgtagaagtt ccaggttttc ttgtgtataa gcgctggata
     6961 gtggcttgcc tggtgtcact tttggcccac cgccatgcat ctttagcgct cttaccgttg
     7021 caacaagaac caccgcgtct ggcatgagac cagaagatct gcattttata tccatcaatt
     7081 tctcgaagcc catgtcagag ccaaatccgg attccgttac tacaaaatca gaaatctcgc
     7141 ttgcaataag gtctgcggta atagaggaat tgccatgtgc tatattggca aaaggtcctg
     7201 catgaacaaa ggcaagttgc tcttctagcg tctgcaaggc attaggcttt agggcatctt
     7261 tcaagagaat tgtcattgct ccagctactc cgatgtcttc cgtagtgaca gctttgcctg
     7321 acttatctag agctacgaca atctttccta tacgccgtcg caaatcatca aggcttgtcg
     7381 caagtgccaa cacggccatg acttcgctgg caacggatat atcgaatccc gtttcgcgta
     7441 gaggcccatt ctctaagcca cctaatccaa caattatgct cctcagtgtt ctgtcattca
     7501 tgtcgacgac acgacgccag agtaccgaat atggatcgat attgagtttt ttcaagccca
     7561 acttttcaaa ataactgtcc gaccaccggc tctcatggta tatgcgtgcg tctatggcag
     7621 cggcgcagag attgtgcgcc gcagacacgg cgtgtgtgtc tcctgttaga tgcagattga
     7681 aatctgtcat tggaacaatc tggctatacc cgccaccagc tgcgccacct tttatgccaa
     7741 aggtcggtcc catggaaggt tgccggatag tcgcaatgga tttcttacca agatatccca
     7801 agccttgagc aagacctatg gtggttgttg tctttccttc accaagaggc gtgggcgtaa
     7861 tcgcggttac atcgatatac ttccctcgcc tcgactgtcc ctgcctgtcc gataagaatt
     7921 ctagccggac tttcgccttg tcggttccat aatgctcgag gtacttttct tccaggccat
     7981 agctcgatgc aatgtcggat atcggcttta tatccgcgga ctgagcaata tctacatctg
     8041 gtggaactgg ggtcgttcga acaacggaca tgaaaaacct ccttttaata aaacccggat
     8101 tcacaaaaga aataggaaag acatgatcaa ctcaacatgc catatttggc taaaggaaaa
     8161 aaacatacag catcaagggg atggtcaagt cttttaaagt agaatttagc acagataacg
     8221 ccgaaagtgt agcgggtcca tcttttggta ggtaagatcg tcgccttcga atttgcataa
     8281 tatatcgaaa gctccaacaa tcataagctc ttagaaagct cggaatagct tttataccca
     8341 taagcggagt ctgcatatct aatggctttt gctattgctc ttgccatggc ttccgctgcc
     8401 atagaaccta caatatctat attgctttct acctctcctg tagctatgga gaaaattgtg
     8461 tctccatcgc ctgaagtatg tgaggggttt atagatcttg catatccatc atgcgccatc
     8521 attgccattc ttgtggccat cggcttgctc aaaatagcgt tggtagccac cactccgatt
     8581 gtcgtatgcg ttgtcagacc ctctggattt gcttgaaaag aaagggcatc ttgcttgttc
     8641 ttcatttctt cataaccatc aagaatatag ctaccagttg gatccaatgt ccctccaagc
     8701 aattctcctg tctcaggatc gtaaacattg cccatgcaat tgacaacaac aatcgctcca
     8761 acaaccagat tgccccttga tgcgcttgct gtaccaagcc cacccttcat tcgctgattc
     8821 aaattccctg cccagcctat caatgcccct gttcctgcac caacattgcc catagacact
     8881 tcttggcttg cattcatgca tgcttctctg cccattgccg gacctggata tgccgatgga
     8941 tcacctactg tcagatcaaa gattaccgct cccgatacta ttggaacaac tagattcagc
     9001 aaatgaaatc caataccatt ttcggataga tattgcataa ctccatcggc acctgccagc
     9061 ccaaaggcac tccccccact caaataaatg gcatgagggg cttgtaccat attgactggg
     9121 ttcaacaaat ccgtctcccg cgtacctgga gaacctcctc gaacatccac tccggctgtt
     9181 tttgcgtttt cgcataagac aacagtgcat cctcgtgctt tgtcctgatc ctgagcattt
     9241 cctactctaa taccaggaac atcggtgatc gaatctctca tgataataga ctcctttgct
     9301 tttatctctt aagcggaact taatccgcat ttttctcggc atcaatatga attgactata
     9361 ccagctatgc cgaacttgct gccaataccg ctccaatagc ggtagaaaac tcagcatctt
     9421 cggggaactc aaaatgaatc ccatacattt cggaaatact accaagaaca tttcttccaa
     9481 ttgcattatt gttgccattg ccagtcacaa taatactatc tttatttcta ctctgcgcta
     9541 caaatacaga gagcattccg attacttggt acaccatatt tataatggcc attgcgatgt
     9601 cttctcttgt tgcgctttcc aacattttgg caaaattcga ggctgtcata tctttagcca
     9661 ggaaactgat gtctgtatcc gcgatatctt tgatcattag atccacacga ctcaaatccc
     9721 cttttgaagc aagatccatt atggtattaa agtcgcttgt cttgatcagt gcttttgcca
     9781 aaccaatgat tgtacctcct cctactccag ttccaccaat atgagcgatt ccattctgat
     9841 ttacttcaat gaagggtgtc ccggtcccaa tattggcgat tatgatatca tcgcgtttgg
     9901 aaagaaattt gccaccatgt ccaatagcca tgatttcttc tatttttact gttggaatat
     9961 caaatatatc ttgatggatt tttgatgcac ctgcaccggt tattgcaatt ctatctatgc
    10021 tttgaatctt ataccctttc tcagtgtcaa gcttgcccaa tgcaccagcc gcagcagtaa
    10081 cagcatccga agcaagcacg cgcattttac acaggataca tccattatca agcaaaacca
    10141 cttttgtact cgtacttcca atatctatgc ccgcgatcat ccctgtttcc tccttcagca
    10201 aatcgaaaaa tggagaacac atcgtcacga tcttgtcttc attcatattt taagaagaac
    10261 ctgtgagcct tgggttaaac gattccgcaa gcgaatcacc aatgatattg aatgcaagaa
    10321 ctgtcaatgc caaacataga cctggatata tagccatcca gggagcttgg attaggtaat
    10381 tacgtgcatc ctttagcatt agtccccaac tggcatctgg gggctgagtg ccaaccccca
    10441 gataagacaa agaagactca atgactattg cctgaccaaa cgtaagagag cattgaacgg
    10501 ctaccttccc cagaagattc gggataatat gcttgaaagc tatcttgaat gaggatgtcc
    10561 ctaaggctct tgctgcctcg atatatcctt cggtctttat agcaagcgtt tctgctctta
    10621 ttaaccttgc aaatatagga ctactgatta ttcccagtgc gagcatcacc tgcacaattc
    10681 catagcctaa gaatgaaaca acagtgattg caagcaaaat cgtgggaaag gaaagcagac
    10741 tatccatgaa tagcatcaat acattgtcca gacttctcgt gcccatacct gctagcagcc
    10801 ctataaccat accgattgca agcgaaatag cgactgtcac aatgcctaca atgagtgccg
    10861 accgagatcc atagatgatt ctacttagga tatctctacc aaaattatcg gtccccataa
    10921 tattttcaag gctcggcgaa tgaaaacgct tcaaaagaaa ctgttcagtt ggattgtatg
    10981 gcgctatcaa tggagcggct attgctgata tcatcaatat aagaaggtac aataccgcta
    11041 ggtcaaacaa gatcttctgg gattttccga acatactctt ctacttcaat tctgtccacc
    11101 tgaaatcgta ggagtccagg tttggagtaa tcctgaaatc tgctatattc gatctcatag
    11161 ctacatagcg atatgagctt ccaaggtata caaaaggtac ctcttttgcc attatctcca
    11221 atgcctctgt atatagcttc ttgcgaacag caaaatcaga gatagtggca gcctctttta
    11281 ttcttgcatc acatgttgga ttgatccatc tgacatactt cttatcggta ccatagcctg
    11341 caagtgttcc atctggatcg agtttccctg tatgcccgat gattgtcaaa tcgaaattgc
    11401 cttctgtata gattctggcc agccagctcg accaatctac aagctgtatc ttgacattca
    11461 tgccaacctt ttcgagcatc tgttgaataa gttcacctgc ttttacatgt agtggataat
    11521 tctgaggcac cgtgatcgtg aattgtctgt taccaacgcc cgcgtccttt agcaactgct
    11581 tagccttatc gggattataa ggatagaaat gagaaaaatc cgtatagtaa gcattgccgg
    11641 tatccatgaa agaagaccca acctttccac cgccataggc aatatccaat acaagctgtt
    11701 tatctactgc gtaatttatg gcttgacgca cacgaagatc gttgagtggc tcacgagagc
    11761 aattcatcgc cataaccagg atcatcgatg tctgttcttt tcgaattgtg atgtttggat
    11821 tggattggag tataggcaga tcttctggat cgacatattc aacggcatcg atagcaccag
    11881 acatcaagcc ctgtatttgt acggatcttt ctggaactat ctggaaatcc actcttgcca
    11941 atttgggtaa gcccttcatc cagtattttt catttctgac catagatatc ttgttatcgc
    12001 gaatccattt ctcgaatttg aatggcccag ttccaacagg ttcagaatca aagttgtggc
    12061 cggactgaat aagatcagct ggaagaatcg ccccccatcc cgaagcaagg ttagcaagaa
    12121 tcggtgcata aggcgcacca agaatgatca cagcggtctg cggatcgggc gttttcactt
    12181 cttttatggc agtgaattcg cttcttcttg gtgatgcgaa gcttggatct cgtatgcgat
    12241 ccaatgttgc cttgacatct cgagaggtca atgcctggcc attatggaac acaacgcctg
    12301 gcctcagctt gaacgtccat gtcaatccat caggagagac cgtccagctc tctgcaagcg
    12361 ctggcacaag cttgcctgtc atgtcaggct cgaccaacgt atcatagaaa ctcttgacaa
    12421 cctgaaaggt caatgttcca gccgttttct gaggatcaag cgtatctgga ttaccggata
    12481 gaccaaatgc aatagatcct ttcccctgtg ccaaaaggga actccctacc ccagataaaa
    12541 gggcaaggaa aaccattccc aaaataaccg atcttttcat tttcttctcc tgagaattgc
    12601 cagtcatttg ccggttctga ttttatgaca ggacacaacg tgccctcttg aaagctccct
    12661 taaaaagggt gcagacaagc tacattcggc atcacccatg ggacattgcg aataataaga
    12721 acatcccgct tgagattttt cttctttctg aggctctctt attgtctttt cgccaagttc
    12781 tccgatattc agactcttta ctcctattct ctgcgctcct cctggtatcg aactgaaaag
    12841 cttttgggta tatgggtgac ttggttcgga tatgagcagt tcggtttctg cttgctccac
    12901 tattctgcct ttgaacatta ctgctatttc atcgcacata taggatacga ggttgagatc
    12961 atggctgatg aatagatagg tcaattcaaa ttcattcttc aaatcgatca agagatttat
    13021 tatctgtgcc tgaatagata catcaagatt ggatgtgggt tcatccaata caagaaaatc
    13081 gggatccata gaaagagcac gagcaatgac aatcctttgt ttttgccctc cagatagttc
    13141 atgggggaat cgacgcagat atgccttgcc gattccgaca agattgataa gatcattgac
    13201 cttgcgatct cgctgttctt tagggatttt gagattcacc attccctctg ataggctatt
    13261 atagatactc attttgggat ccagcgcgct attcggatcc tggaatacaa tttgcatcct
    13321 gtttcgaaat cttctcaatt ccttaggttt taacgaccca agcggcacat catcgaaaat
    13381 cactgaaccg gatgttgggg gatcaagata cataagcgct cttccaaacg tcgtcttgcc
    13441 acatcccgac tccccaacaa gcccaaaaat gctctgcttt tttatattca ctgaaacttc
    13501 atcgacagcc ttaaagccat gttttgatcc agctaggccg cgtgagcgat atatctttgt
    13561 tatattcttg ctttctataa gatctgccat atcttacctg cataataaca tgcaactgta
    13621 tgtgcatttt catatgccac ataacttgga atatcgttga agcaatcctc tttcgcaatt
    13681 ggacatcgat ttgcaaacgc gcagccattc cctatttctg ccgcatccgg aacctgtcct
    13741 ggaattgtgg ctagtttcga tcccttcttc ttagcatctg gaatcgaatg cataagcagg
    13801 cgagtataag gatgcagagg atttctaaat agctcttcct ttgtggcgct ttcgacaatt
    13861 cttcccgcat agataacaga gactcgatca gcaatttcgc ttacaaggta aagatcatga
    13921 gaaataaaca acacaccaag ttttctcata tctttgagtt ttttcagcaa agcgagaatc
    13981 tgaacttgca gtgtcacatc gagtgccgtc gttggttcat cagctataat aagatcagga
    14041 tcgcaggata tggccatggc aatcatggct ctttgcttca tccctcctga taattcatgt
    14101 ggatagctat cgataacctt ctcatccaga ccaaccaacc tcaacagctc gacaactctc
    14161 tcataagcag atcgcttctt cattcccata tgaagcataa gcatttctat tatctgctcc
    14221 ccaatcttga atgcaggatt cagatacttc gcaggttcct gaaatatcat agcaattttc
    14281 ctgcctcgat aggttcgcat ctgatcctct ggtaatgaca gcaaatcctt cccatcgaag
    14341 tatatgtgtc ccgataaaat tctcgcagga ggaataggta ggagcccaat tatgcatgtg
    14401 gatgtaactg tcttgccaga tcctgactct ccaactagtc catggatctc atttcctaaa
    14461 atggacagat ttatgcctcg cagaacagtg gtctctttct taccaagaga aaatgcgact
    14521 ctcaggtctt ctatcgataa aagggaggcc attctttcca tttagctcag ccttatctta
    14581 ggattagcaa cgctgtaaag tatatctgca atgaaattta taaatgaaaa caagatggcc
    14641 acaaaaacaa cacctccctg aatgattggg aaatcgcgct gatagatgcc agataagaag
    14701 agccttccca atcctggcat cgagaaaacc tgctcgatta tgattgcgcc gccaagcata
    14761 tagcccagtt caatacttgt aacggtgatt atcggcaaga gagcattctt tagtgcatgt
    14821 ttgtatttta ccatacgctc agtaaggcct tttgccctcg cagtgataat gtattctttg
    14881 gatagctcct ctaccatgga ggctctagta tggcgcaaaa ttatcgctgc gcgaccaaat
    14941 ccgagcgaga ctattggcag aatataatgt gcaaaggtct ccgaaccaaa aagcgggaaa
    15001 attggtatct tgatagcaaa aatcaaaagc aaaattatgc ctaaccagaa actgggaatc
    15061 gccataccca attgggaaag aaccaatcca aagtaatccc acgccgacca tctttttact
    15121 gcggataaaa caccaagggg caatgcaacg acaatactga acgcaatccc gaaaatcgca
    15181 agtttaagag taagaggaaa tcgctctaaa atgagtttaa gaacaggttc cccagagatc
    15241 atggaattgc cgaaatcaaa gcgcaccatg tgccagataa aatctccgta ttgcacaaga
    15301 ataggacggt ccgtacccaa ccttgttcgg attgcctgca catcggcagg atttgcatca
    15361 atccctgcga taatcaatgc aggatcccca ggaatgacgc gaataacaaa gaatacgatt
    15421 gtagcaatta tgaaagctga aaggataagc ctcaagaatc ttctagcaat aaagcgaatc
    15481 aaatccatga ctgcctacta ttacatcatc tattgatatt agaatcgatt gtatagatac
    15541 cgcttattat tcattgcagt actcccttct atttgcgtaa atgacgaaaa tccaatctaa
    15601 tttaaaacta tactagccgt actgactgct gtcaattaag actatttctt tgctttagag
    15661 aactgtggtg gcctttattc ctaaaaacct ggtttgtagg cattggtata atcgatgtca
    15721 gagactttga ggtgcatcga ctcatcccgc ggtttggctt ttttcttccg ttggccttgt
    15781 tttcttcttt taggaggagg aggagctggc aagagatttt cttcttgaag cacttgttcc
    15841 attgcctcct ctagtgccac tcgaggcaag ttgaggggcg atagaaatac ccgaatatcc
    15901 gagaagatct gatgcctgaa tgtgacgggg tctttcgata tatctctgag aaaagagaaa
    15961 tccttgatcc tttggcggat aaaccaggcc agaaatggag atatgagcct tttgtccgtc
    16021 tgaataatct cagcgggggt tgcgctgttc aataagcttg taccctcagg cacctgagct
    16081 agcatatcca tggaagccag gtctcttaag aatgcttcct taaatgcctt atctccatgg
    16141 attgaggcat gaacctcagg cgatagatac tgcaataggc catatttatc caatgcctgg
    16201 aaaatctcca ccgacttccc tgtggcaatt atcttgtaga actcttccgc aagcctcgag
    16261 ggacttacgg aatcaagaag tgacgcatcg cgtcgaatag ccaatctcaa tgaaaatgga
    16321 attacaaatt gattggttgc agcgtattta ataccgcgca ccatacgaac aggatcttcg
    16381 ctaaagatcc tatccagagg aataattgcc tttaacttgc cggaatggat atcatcgact
    16441 ccgccgacaa aatccagaat ctccagggca atcggatcca agtaaagcgc attcacggtg
    16501 aaatctcgcc ggagtacatc ttcgctgatg gtcccatatt cattgccgac agaaccattc
    16561 gacagggaac gaaacgtgct cacctcgtat atcttcctat tagaatagac atggacgagc
    16621 ttgaagcgcc ttccaatgat gcgagaatta ctaaatattt tccggatacg attgggcaag
    16681 gcatcggtta ctatgtcgaa atccttgggc cgccttccaa gcaataaatc gcgtacggct
    16741 cctccaacga tatacgctga atatccatgc gaaactagcc tattcactat agctagcgct
    16801 tctctatcga tgcaagatat gtcgatgcaa tgatcttctt ggccgtatat tcgcgctttt
    16861 gcctttagac taccatttga attgcgaaag taccgcattc tcacaaagct gctccaacat
    16921 gcacgctcat acagtacaaa aattcattga atgtgtcgag agaaccagtc gatgatgtcg
    16981 ttgattactt cctgcttatt gatttcattg atagattcat gcctgccccc atcatagcac
    17041 ttggattcga aatcaatcat gccaagaagt ttgaatctct gtccaagttt ttcatatccc
    17101 tctttcattc ctattaccgg atccgcagaa ccacatagca agaataaagg aagggatgga
    17161 ggaatgcttt ccaaaaaacc attactatag acttttctaa caccatggag aagatcgcga
    17221 tagaatccat aagtacagat aaagttgcat ttagggtctc taatataggc atctacgata
    17281 gccgaatccc tggaaagcca gtcgaatttt gtcttcgcat ctgttacgct ctttgcatat
    17341 tggccgaagg tcatctgatt tgggagcttt gcaggagcat gaaagccctt taatgctcct
    17401 cccatggaaa tcatcagtcc tcctatctta tccatgaata gtttctgctt ttcgggtgga
    17461 gaggaaagca ccgctccgtg aaggtatttt ccgtaggtcg cgatcagcgc cataccaata
    17521 agggctccaa aggaatgccc aaaataaaaa agtggtaatt ctcccatgca gctagaagcg
    17581 tagttcccca tttcaatcaa atcttggacc acacgttcga atccatcctt gtcggctaga
    17641 taacctctat tggtgccaga atcggtatct ccatgacctc ttaggtctgg aatccagaca
    17701 gaaacaccaa ttggcgtaag agcctcggca agatgcataa aacgctcttt gtgatcattc
    17761 atgccatgac caactataac cacggctttt gcttcatttt tggggtcgaa tcgcagataa
    17821 gccagttctt tgccatcgct cgctctgaat ttttccatct aagcccctca atattaaatg
    17881 ctgatctttt tataatgatc ggtatatttc attctcattc aatgattatt gtgctaagtg
    17941 ggctaaatgt ccagccttga tcttcaatta tacgcttctt tttcttcagg tctagaaaaa
    18001 accgattttc gcgaatacta tagaataaga gaatatgccg aaggaggaca atatctgatt
    18061 attctttcga gcccttatgt gcgcagaaca attgcaatag gcagagcaat agggcgtgcg
    18121 gcgcctggtg gatctatcgt tgcattccgc ggagatcttg gtgcaggaaa aacaacgctc
    18181 gccagaggag ttgctcaagg attaggaata gccgagccta taatatcacc cacatacacg
    18241 ataatagccg aatatcaagg ccgcctacat cttgtacata tcgatgcata tcgtctctcg
    18301 gatgaggatg aattcgtgca gaccggaggc gaagagcttc ttggttcgcc cggctcactg
    18361 agtctcatag aatggagcga gagaatcgcg ggagtattgc caaacgaaac ccaatacatc
    18421 accatggttg tcaaagaagg gggaaacaga attatcctaa tcgaaggtga ctggatcgag
    18481 aatatcgact ggcagcgttt caagattccc tattctatcg atttacagct tgcttcaacc
    18541 ttgtcaaaaa ggtaccaaaa tgactgtcct gtcgattgat acgagcattc cgacactgag
    18601 ggtcggtgtt tcaaaagaaa aaacgcttgt catatctgct ctggagccgc ctgtcactca
    18661 tgctgaggcc attcttcccg cggtggaaca agtacttcaa aaagcaggaa ttacgataaa
    18721 taatatcgaa ttactcgcta ttgcaggcgg ccccggctca tttacaggtc tgcggatagg
    18781 aatcgcaacg gcaaaaggac tttctctcgg acttggaatt cctatggtgc ttgtccctac
    18841 cctggatata tatggatggg cctggagaga actcgaaggc attgtcgtgc ctgtcatcga
    18901 tgcacgaaag catcgagtct attgtgctcg ctattctttc ggtcaaagaa taggcgattg
    18961 gatggatatc gagctcacgc aactaatatg gaagatacat ggcgaagata aggttaattt
    19021 tgtaggtccg gatgcagagc tggccgaggc tatctgcctt gaaagagaag gatggatatt
    19081 gcatagacca aaccttgaaa aggaaataga atctctctct gtcctgggta tcaatatata
    19141 tctggaaaag ggcggtgcca atgataccgc aggacctatt tatctccgtg aaccggaaat
    19201 aggcactcca atagcgcgct gattaaatct cctttttata agccctggtg cgcttatgtt
    19261 ggcgcttggg ataatccttc catatgctct gaactttgat attatcctcg agctccagat
    19321 tggtttctgc atattcgaca cctgcttcca tggcactgcg ctgaagctcg ttcataacca
    19381 gggcaagcac gcctcgagac tgatattcgg gacgaacagc tacgagatat agatcaagca
    19441 cctttggatt attcagcgct tttaagacat gataccagcc tgttggaaag agtctgcctc
    19501 gcgatctata gaaagcttcc gaaagactag gcatggctat gccaaatcct attaggcgct
    19561 catctttatc taccagaatc ttggtaaatc gtggatccac aaagccaaga tactgcttga
    19621 tatagtcctc tacctggcgc tcgctcaaag gcgttgttcc atagagatcg ccatatgttt
    19681 catctattag cgcaaagagc tgcttgccga atttttcgac gatgacgcgc ttgtccttcc
    19741 attcgaacag ccgcaccccg gagcgttttg cgagcaaatc agccacatgc tggactttct
    19801 ccggaatctc cttaggggtg aagatttgga attgaagcca gtccacatct tttcggtaac
    19861 caagcatctc caaatgctga ggataatagg gataattgta gatcgtagct aaggtcgcac
    19921 gctcattaaa gccttccacc agcatgcctt cccgatcgag gtcagtaaaa cccaaaggcc
    19981 catgcagcgc ctccataccc ttcgaagctg cccattcctc aacagtctta aagagcattg
    20041 cggagacctc ggaatcatcg ataaaatcga tccagccaaa acgaccccat gcctttcccc
    20101 atttttctat aaagcggtga ttgattatgc ctgcaatgcg gccaacgatt ttttcgtctt
    20161 tccatgctgt ccaatattct gcctcacaaa attcgaaagc cgcattctta tctttgctta
    20221 atgtacgcag ttcatcaaag cggggagcgg gtacatagtt gggttgatcc caataaaggc
    20281 gcacaggaaa atctatgaat cgtttcagat ctttccggct ttcgacctta ctaatctcta
    20341 caggcatcct ttcccccttt ttcagttctc cattcttcga tgaaaagctc ataggctgcg
    20401 attgtggcct ctgaaattag atctccaagc aaaccacggc ctaaccaatc atttccatct
    20461 agcacatata ggtgagcaag ttggttttct gcgtctgcaa tcgaaaaggc agggtcgaca
    20521 gcacgacgat gtcctaggcc attcaatgtt ccatcgaaca cttccatata tttttgatca
    20581 ctccagctgg ccattcaaac cccaacttca aaatcctgca aattatagcc ttagaggtca
    20641 aaatcttaca aggtattgac caaggggcat ttttgagata tacaaccacc tgcatggcgg
    20701 gatagctcag ttggcagagc aagcggctca
//
LOCUS       DAOOWY010000025        20595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2588_length_20595_cov_10.673564, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000025 DAOOWY010000000
VERSION     DAOOWY010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 20595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20595
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2588_length_20595_cov_10.673564"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(508..3063)
                     /locus_tag="PK105_03445"
     CDS             complement(508..3063)
                     /locus_tag="PK105_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU60794.1"
                     /translation="MANYIVIGGVAGGASTAARLRRNDEHAHIVLVERGAYISYANCG
                     LPYYTGTVIAERSKLFVMTPQSFQETLNIDVRINTEATAIDPKSKTVTLIDTTNGKTY
                     QEPYDELILSPGAEPIRPPIPGMDLEGIFTLRTVPDADAIKAWLDTLNPERAVVVGGG
                     FIGLEMAENLAHRGVKVAIVEALEQVMAPLDFEMAAIVHRHLREKGLELRLRDGVKAF
                     EKQDNRILVRLASGDTMLTDMVLLSVGIRPDTKLAQDAGIATIPAGKPGAGAILVDEH
                     FHTNLPYIRAIGDAVAFKNPITGEVGVVPLAGPANKQGRLVADGVVYGDEKTPPWKGT
                     LATSIAKIFDLTVAATGANEKVLDRIGKPHTGIIVHPNNHAGYYPGVVPLSLKVVFDP
                     ESGRLYGAQAVGYDGIDKRIDVLSALIGKKGTVADLTEFDQAYAPPYSSAKDPVNMAG
                     FVADNVLAGRSHLIDYREFEKLRSEGAFVLDVRTLEEFELGHIPGAICIPNTELRKHL
                     SELPKDKIILIYCSVGLRGYLSERILRQNGWSSVYNLSGGYKTWQVVMEKQDSSGALA
                     AQASLYGTTQQGLQYAMTAYREGTGMPEQKIINVDACGLQCPGPIMRLKNEIDAVEPG
                     TRIIITASDPGFSRDVQSWANLTGNLLISLEEKGGTIQAVIEKGATISSVVRKAEGQH
                     SQQDSEGFKMLQTSQGATFIVFSNDFDKILAAFVLANGAVAVGKQVTMFFTFWGLSVI
                     KRPEKPSVHKDFMGRMFGIMLPKHAGKLSLSKMNFAGMGVQMMKSRMKAKNVDQLEQM
                     IQSSIASGVRMVACQMSMDIMGITKEEFIDGIEVGGVATYMEAASKSGVNLFI"
     gene            complement(3183..3557)
                     /locus_tag="PK105_03450"
     CDS             complement(3183..3557)
                     /locus_tag="PK105_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU60795.1"
                     /translation="MQQQLCVLKDILRSIAQLEDQIQEHYHLSVSEALVFCALGDGHS
                     TAASLAERVGLSTSRICRIIASLERKGLIERFRDNHDRRQWENVLTNQGKQKLLMMQE
                     KGIEMPQWLLSLASSKDLLPFH"
     gene            3800..3922
                     /locus_tag="PK105_03455"
     CDS             3800..3922
                     /locus_tag="PK105_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60796.1"
                     /translation="MKNRKIKVDLLHQVFVRLDKGERIISILVAARFKKAKSAD"
     gene            complement(3939..4012)
                     /locus_tag="PK105_03460"
     tRNA            complement(3939..4012)
                     /locus_tag="PK105_03460"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3976..3978),aa:Ala,seq:ggc)
     gene            4149..5297
                     /locus_tag="PK105_03465"
     CDS             4149..5297
                     /locus_tag="PK105_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOU60797.1"
                     /translation="MRAIAACPIGLEKVLANDLAHLGFHDVGRSAGRVLFNVSEQSLA
                     EDLAKANIGLRTADRVLLVVGEFKAFDFDGFYQGVSSISWERFCTKETKVIIERARIR
                     NCVLHSQVTIQSMAQKSIYASLMKQFHIRTMPETGDILEVRIYGDNNWWQIVVDTSGE
                     ALSRRGYRRLTHMAPLKETIAASMLFLAGWSRARPLIDPFCGSGTIAIEAALYACNVA
                     PGMRRRFAFESFSGIDNNKIEDVRIAFRKQARKDVRTDIRASDIDEDALALARKNATL
                     AGISEFIRFVKIDARQIEPFGSRGVLLANPPYGQRMGSADKARSLYEELGPIIERFLE
                     AGWECGFLSSISSFGDTVGIGPTSVREIVSGQEKIYFNWFSGTEGLKT"
     gene            complement(5304..6008)
                     /locus_tag="PK105_03470"
     CDS             complement(5304..6008)
                     /locus_tag="PK105_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOU60798.1"
                     /translation="MLKEEFIQKSPVRIFEKSIEGGLHAGNIGILASKKGIGKTSVLV
                     QIALDKLMQGEKVIHVSFNAHTSYIISWYENIFNEIARRKNLENIEEIKEQMIRNRMI
                     MNFMQENISVDQIIRTIKAIMSDGSFNAKTLIIDGFEFTRATVERFEKVKAFLSETQL
                     EGWYTCTLADTEPVLDSKGIPIVLRDIEPYISVIVLLEPKKDYIHLRVVKNHESLERG
                     DMPLMLDPRTLLIAEA"
     gene            complement(6070..8157)
                     /gene="fusA"
                     /locus_tag="PK105_03475"
     CDS             complement(6070..8157)
                     /gene="fusA"
                     /locus_tag="PK105_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU60799.1"
                     /translation="MAFDVHKMRNIGISAHIDSGKTTLSERILFYCRKIHAIHEIKGK
                     DGSGAAMDSMELERERGITIQSGATNVQWKNHSINLIDTPGHVDFTIEVERSLRVLDG
                     AILVLCSVAGVQSQSITVDRQLKRYHVPRLAFVNKCDRMGANPLKVKAQLAEKLGLNP
                     AFITIPIGLEDKFEGVIDLIDMRAIYFDGPNGEDLRYAEIPMHLLDDAERYREELLEA
                     ASMFSDELAEAYLDGNPTKELLIKAIRKGTIQEKLVPVFVGSAYRNKGVQPLLDGVIN
                     FLPDPSERQNKALDLSNAEAEVELQANDQEKTVALAFKLEEGQYGQLTYVRIYQGMIR
                     KGDELYNTRARKRFRVGRLVRMHSSSMEDIQEGNCGDIVALFGIECASGDTFCHPDLN
                     YTMTSMYVPEPVISLAITPKDNKSSDQMSKALNRFTKEDPTFRTYVDPESNQTIIQGM
                     GELHLEVYIERMKREYKCEVTPGMPQVAYRETITQRADFNYTHKKQTGGSGQYGRIAG
                     YIEPFKEGSYEFVDLIKGGAIPNEFIPSCDKGFRAAMEKGALIGFPITGVRCVINDGQ
                     SHPVDSSDIAFQFAAIGAFREAYDKAKPAILEPIMKVVVETPNEFQGNVFASLNQRRG
                     IIMANTENSIFSRIEAEVPLSEMFGYSTVLRSLTQGKAEFSMEFEKYARVPAALSEQL
                     RKDYLEKHKKGHK"
     gene            complement(8387..8803)
                     /locus_tag="PK105_03480"
     CDS             complement(8387..8803)
                     /locus_tag="PK105_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU60800.1"
                     /translation="MTKEIQMSWLEDMAFESLLDGHRLVLDADDNSGGKDRGPRPKGL
                     ILAALGACTGMDVISILKKMREPITWFNMKLSGKVAEEHPKKFTSFKIIYQFKKSDGL
                     KPENVHKAVELSQTKYCGVSATLKDGHEVAWEIEYI"
     gene            complement(8922..9482)
                     /locus_tag="PK105_03485"
     CDS             complement(8922..9482)
                     /locus_tag="PK105_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60801.1"
                     /translation="MRRGIKDISLGNVMLAIAAVILLAGIPSVNAQSTSKTSVSIQIM
                     VYVPAILNLTLDFSTSGATELVGYLGDTSDANGKGFELKSNSVVSLGAAHIISNLSSG
                     YSIVVQSMNGCALKNQDSQGTVKYDLLLGGIPALRQGDSFKLNCCGTTPMEGQMLPVS
                     IALGNVPSNATPGLYADNLLFNIMAN"
     gene            complement(9727..10746)
                     /locus_tag="PK105_03490"
     CDS             complement(9727..10746)
                     /locus_tag="PK105_03490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU60802.1"
                     /translation="MKQRFMPLAFVLTLLAVPIQSCSAQTARPDGMYAIMKTNRGTIV
                     ITLEFEKAPMTVGNFVGLAEGSLDATKGKHFYDGLAFHRVVANFVVQGGDPVGDGTGG
                     PGYRFPDEIVPELKHDGPGVVSMANAGPNTNGSQFFITLEAAPWLDGKHSVFGKVIEG
                     MDVVKQIQKGDRIESITIQRVGTKAKSFDSSQQAWDQRLSAAYATLNALNKQKRDSDL
                     ELIAQKWPDLQVDENGIFQKIVRKGFGSMPAPGSKVSVIYKGMLLDGTVFDQSALSGS
                     PFAFTIGQGEVIDGWDLVISTMRKGEKRMIIIPPELAYGAQSIGDVIPPNSFLVFEIE
                     LVSIQ"
     gene            10857..11342
                     /locus_tag="PK105_03495"
     CDS             10857..11342
                     /locus_tag="PK105_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOU60803.1"
                     /translation="MSEYRRPTWDEYFMEVANAIAKRATCDRGRSGCVIAKNNQILAT
                     GYVGAPSGLPHCDDVGHQMRKMLHEDGSITEHCVRTVHAEQNAICQAAKKGVAIEGAT
                     LYCRMTPCRTCAMLIINCGIVRVVAEYRYHDAEESEAMFKMAGIVLEYVHREVLQYSR
                     Q"
     gene            11434..12261
                     /locus_tag="PK105_03500"
     CDS             11434..12261
                     /locus_tag="PK105_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOU60804.1"
                     /translation="MGITIVFLGEIVGKTGVFTVKSTMPEIRQRFEPDFIFANANSAT
                     GCAGLGIQHAVYLRKLGIDCITMGEAAYYKPDMTEFYPKAGWVLRPANLPEGDPGRGW
                     RVFEKNGKRMAVVMLLGQSGFARIHADNPFLAIDRLSNFLRKETACITVNFHAATTAE
                     KLSMARHTDGKVSAMLGSGGKVLTADARILSKKTAAITDLGRTGSMLSVGGLDPDAKV
                     KEFLTGIPSWSREASAQPEAQGTYICFDDDGKAISIEPFRISGKEIVDEGDSNSKKN"
     gene            12230..12646
                     /locus_tag="PK105_03505"
     CDS             12230..12646
                     /locus_tag="PK105_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOU60805.1"
                     /translation="MREIAIVKKIKGDIVTVSIEMQEGCAVCVNNGRCKIRKSDLSVY
                     NKDHIDIDEGDRVVVEIRTSEQAKSAFWVLGFPLIMLFVGYGFGAFIFKGSGEGPAVV
                     CAGLGFIFAMGIGMLIQYRRKLKSFPYILGKEGEIL"
     gene            12979..13881
                     /locus_tag="PK105_03510"
     CDS             12979..13881
                     /locus_tag="PK105_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU60806.1"
                     /translation="MFHAKRPEEAADQALVAHLSRQTSHRGGSISPKGAFPKDIDGSH
                     TKKTELAIYIDEVSKSFGNHSVLHKVSLAVPRGSIHGIMGKSGAGKTTLIRIAGLLEK
                     PDQGSIWYEGIGMPVHELHGRAMLEARRQVGFVFQSFNLFSSRSASGNIAFPLEAAGW
                     PREKIRARVEELLEFVELSDKANRPAGRLSGGEKQRVAIARALANSPSMLLSDEATSA
                     LDPETTRAVLDLIRSLRDRLNLTVIMVTHQKEVIRRICDTVSVISGGSIVEQGSVAEV
                     FSNPRSEAAKAFLKEEEEKGHSYV"
     gene            13874..14539
                     /locus_tag="PK105_03515"
     CDS             13874..14539
                     /locus_tag="PK105_03515"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HOU60807.1"
                     /translation="MSEILRLLIRPLGETILMIAASTLISTILGGGLGILLFTLSQDR
                     FAGKYPGALLVKRIADRAINMFRSFPYVILMVLVLPLSRLIVGTSLGTIASIVPLSIA
                     AIPFLARIVESSLSEVDSGVIDAAISCAASKTRILFAILIPEALPSLISGLTLTMISL
                     LGYSAMAGVIGGGGLGDLAIRYGYQRFRADVMAVAVVAIVALVELIQWTGSMIIKRIK
                     AAR"
     gene            complement(14523..15242)
                     /locus_tag="PK105_03520"
     CDS             complement(14523..15242)
                     /locus_tag="PK105_03520"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HOU60808.1"
                     /translation="MEKKENTEVLDDTFIAAARDYRMLLDKDYPIDASLKLVGDRYRL
                     SHNGRMMLYRGILSLKASQINLAKLVRSPWERASLGVDGYNILFTLMNYMHGHPMFIA
                     TDGFLRDVGGAHGRIADHRGFERMAEITAEYLAALNLQSLTFFLDAPVSSSGRQAGFL
                     REAFAQRSINAEVRLENGVDHFLAYWEGELIATADSAIIGRTNAHVFDLARAILENKF
                     DARFISLARFLEPSYPISAPP"
     gene            15482..16984
                     /locus_tag="PK105_03525"
     CDS             15482..16984
                     /locus_tag="PK105_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU60809.1"
                     /translation="MIHESSSIYQIFLRNFTKEGTFCAAIPELKRIADMGFEWIYLTP
                     IHPIGEKARKGTLGSPYAIKDYRKINPELGDEDDFAAFVQEVHAHGLKLMIDVVFNHT
                     SPDSVLVHEHPDWFLRAADGLPWRKCENWSDVVDFDYRSSPHLWLELIDTLTIWRDRG
                     VDGFRCDVASLVPSEFWRQARQRVNQYDPGIKEERAQLLWLAESVHPAFLRKMRLLGY
                     NSWSEPELHVAAFDLTYDYDGWERLEKIWEQGMPFERYLEYLYVQETLYPKGAKKLRF
                     LENHDQERAAHRFGNGAKLRAMTPFYQFLPGVAMSYMGQELALEHMPSLFEKDPIDLE
                     RGDKEFERYFIASLRATKEAKKEAPFFSWLIKGDAALQDITDQDMASRDKAPRTRTVQ
                     NRTSNNCAVREEVIYCLRSVEPAISPYLIDRSMIIKGNYLLVIQPELDASGPKSSGVK
                     SQRRISFAEESMPRALFDIEGTDLLSGKEVRIAEGDILPRIPASLIRLKD"
     gene            complement(17014..17298)
                     /locus_tag="PK105_03530"
     CDS             complement(17014..17298)
                     /locus_tag="PK105_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOU60810.1"
                     /translation="MKATKETLDHIRKKKAYFVGKPNPLMMRHALRTLGTTYEETIIV
                     GDRMDTDIIAGIESQIETILVLTGVTDEKDMPKFAYAPTFVLESVSEIPQ"
     gene            complement(17342..19522)
                     /gene="ppk1"
                     /locus_tag="PK105_03535"
     CDS             complement(17342..19522)
                     /gene="ppk1"
                     /locus_tag="PK105_03535"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOU60811.1"
                     /translation="MKELPMLNRELSWLDYNARVLQEGLKGSLPLLEQLNFMCIFASN
                     LDEFFMVRVAAVKTAVQTGQTGQTNEEWAGMEPRALLEAIHEKVQASYDKLHMHLHGT
                     ILPSLVQHGIQFVQEKDWTTKESRYLERLFAERIYPLLTPLRLEDNHFPSTGSLQVHV
                     AFRLEENDSVAIIQVPKNIDRFIILPQYLSLEPDNSANSMVIAAFRVALLEDVILAFG
                     SKLFAGLKISSGLIFKVNRDADFSVDEDRDDDFLTAMEEVLTGRQNSTPVRMVYSGED
                     QVLIDALRQSLSLDALDLYQADWLIDLGRVDELCNPEFYKAHGFTPDRVLFFQPWKPV
                     QLFEEKGSIFDWIDEHDRFVNLPYESFDVVQRFIEESASDPSVLGIKMTLYRTSGMNS
                     PIVHALAKAARSGKQVVVLLELKARFDEEKNISWATELEQAGAIVTYGVARLKVHAKA
                     ALIIRKRQDGSIARYIHLSTGNYNEKTARNYVDFCLFTANPSLCTDIALFFNILTGYS
                     NIQSLSLIALSPFDLKDRIIGFIEREAAQTTPESPGFIIAKLNSLSDPDLIAALYRAS
                     AKGVQIKLNVRGVCTLIPGMAGLSENIEVCSVLGRYLEHGRMLYFRNGGSEELYISSA
                     DWLQRNMKKRLELLFPVLDKEIKARCKDILNIYFADNTHAYRLKNDGTWMDLSLSRRP
                     EDPLLYAQEKLHKMTKKTVRNLEEKQEILQVRRSKGKSIGQSIP"
     gene            complement(19556..>20595)
                     /locus_tag="PK105_03540"
     CDS             complement(19556..>20595)
                     /locus_tag="PK105_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU60812.1"
                     /translation="LSIGTLRIDEQIRESAQQPRQFIEMYLESNIRTACDILAEDLQL
                     DSLRIFVLIGADARFAANCLSEKVLGNYAVLDRKQFIEFADYVANMSTEECMAQYHLS
                     WNEVENYTAGLTIERMFLERTGADTIIVPNISIREGILLAQVQGMNKKIEEELRRQVV
                     ASARALGKRFHYDEAHAAHVRNISLKIFDALKKEHGLGIHERMLLEISAILHDIGTYL
                     RTSGHHKHSEYIVANSEIFGLNMDDINIVSNVVRYHRKSQPLSTHVNFIALPRESRII
                     VLKLAAILRVADALDRSHLGRIKELSFERTEDRFIIRTSESLDFSLEKLSLAEKGDMF
                     EDVFGLIPVLI"
BASE COUNT         5311 a         4670 c         4850 g         5764 t
ORIGIN      
        1 cagcatattt gtcagcatct tgagctccta tcccgactat aaccatcagc atcattattg
       61 aaagaataaa agtcgagata ccctttttca tcagtttcct cctcttggaa attgtctatt
      121 agatgtcttt atttggatga atcgtaacat cagtatgatt aaaacatcag agacctgcgc
      181 tgtcaacagt tttattactg ctacgaacct tgtggtttgc ttcgatctat tgaaccgccc
      241 caggtttgct atctcgatag agagcttaga aagcaccttg cggatcatcg cagagaaacg
      301 gtgtgctttg caaggaatgc gtccaatatg ctgcatagac ttgtggtgta tatatataag
      361 aagaaagcag attatgtgcc tggctatgct tgtgcatgag aaggcttcct tatgccattc
      421 tcaatcctca taaacaaggt tcgtagcagt atggaattta tcctttatct gagcactttg
      481 aaaaaccgcc aaaaggtcca gagagtttta tataaataaa tttactcctg atttgcttgc
      541 tgcttccata tatgttgcga caccacctac ttcaattcca tcgatgaatt cttcttttgt
      601 aatgcccatg atatccattg acatctggca agcaaccatc cggactcctg aagctattga
      661 tgactgaatc atctgttcca gttgatccac attcttggct ttcatacggc tcttcatcat
      721 ttggactccc atgcctgcaa agttcatctt ggaaagagaa agcttccctg cgtgttttgg
      781 aagcatgata ccgaacatgc gacccataaa gtccttatga accgaaggtt tttctggtct
      841 cttgattacc gaaagccccc agaatgtgaa gaacatggta acctgttttc ccaccgcaac
      901 ggctccgttg gctaggacga aggccgccag tatcttatca aaatcgtttg aaaagacgat
      961 aaaggttgct ccctggctcg tttgtagcat cttaaagcct tcagaatctt gctgagaatg
     1021 ctggccctca gcctttctaa caacagagga aatagtagca cctttttcga tgaccgcttg
     1081 aattgtgcct cctttttctt caaggcttat caaaaggttc cctgtgaggt ttgcccagga
     1141 ttgtacatca cgggaaaagc cagggtcact cgcagttata ataatccgag ttccaggttc
     1201 taccgcatcg atttcgtttt ttaaccgcat gattgggcct gggcattgca gaccacaggc
     1261 atcaacattg ataatttttt gttcaggcat accagttcct tctctgtagg cagtcattgc
     1321 atattgcagt ccttgctgtg ttgttccata gagtgaagct tgtgcggcga gggcaccaga
     1381 gctatcctgt ttttccatga cgacctgcca ggttttatac cctcctgaaa ggttatagac
     1441 actagaccag ccattctgtc gcaaaattcg ttccgaaagg tatccgcgta gtccaacact
     1501 acagtagatg aggattattt tgtcttttgg tagttcagag aggtgttttc gcagttccgt
     1561 gttggggata caaatggctc ctggaatgtg gcctaattca aattcttcaa gggttcgcac
     1621 atcaagtaca aaggcacctt cactgcgcag tttttcgaat tctctataat caatgaggtg
     1681 acttcgccca gcaaggacgt tgtctgcgac aaaaccagcc atattcaccg gatcttttgc
     1741 tgaagaataa ggcggtgcat acgcttgatc aaactcggta aggtctgcaa cggttccttt
     1801 tttgccgatc agggcagaga gcacatcaat acgtttatca ataccatcgt atccgactgc
     1861 ctgggctcca tataatcggc cactttctgg atcaaagact actttgagtg aaagaggtac
     1921 gactcctgga tagtatcccg catggttatt tggatgcaca atgattccag tatgcggttt
     1981 ccctattcga tcaagtacct tctcattcgc tccggtggct gcaacggtaa ggtcaaagat
     2041 ctttgctatg cttgtagcaa gagtcccctt ccagggtgga gtcttttcat cgccatatac
     2101 aacaccatcc gccaccagtc gtccctgttt gttcgcagga cctgctagtg gtacgacccc
     2161 tacttctcct gtaataggat tcttgaatgc tacggcatcg cctatggcac ggatatacgg
     2221 aagattggta tggaagtgtt catcgacgag aatcgctcct gctccaggct ttccagctgg
     2281 aattgttgca ataccagcat cttgggcgag cttcgtatct ggtcggatac ctaccgagag
     2341 caggaccatg tcggtaagca tagtatcccc cgaagccaat cgcaccagaa tacgattatc
     2401 ttgcttttca aaagccttca ccccatcgcg caaacgaagt tccaggcctt tttctcgcaa
     2461 atgtcgatgc actatagcag ccatctcaaa gtcaagtggc gccattacct gctcaagggc
     2521 ttccactatg gcaactttta ctccacgatg tgccagattc tctgccatct caagaccaat
     2581 aaaaccacca ccaaccacga ctgctcgttc tgggtttagt gtatcaagcc atgctttgat
     2641 ggcatctgca tcgggaactg ttcgcagagt gaagatacct tccaggtcca tgccaggaat
     2701 aggaggacga atcggttccg ctcctggaga aagaattaat tcatcgtatg gttcctgata
     2761 cgttttacca ttcgttgtat caataagtgt tactgttttg ctttttgggt caatagcggt
     2821 agcctcagtg tttatacgaa catcgatgtt tagtgtctcc tgaaacgact gaggggtcat
     2881 cacaaagagt tttgaccgtt ctgcgatgac tgttcccgtg tagtagggca ggccgcagtt
     2941 tgcataggag atgtatgctc cccgttctac aagaacgata tgagcatgtt cgtcatttcg
     3001 tcgaagtcgt gcagcagtac tggctccgcc agccactcct ccgataacaa tataatttgc
     3061 catatattac tccttgttat gggcacctcg ggaaacccca gcttttagag gcacccttgc
     3121 tattttccca aagtcttggg caagttaaaa cttctaaaaa tcgtagtttt tccaaatttg
     3181 cactaatgaa aaggcaacag atcttttgag ctagcaagag aaagcaacca ttgcggcatc
     3241 tcaatcccct tttcctgcat catcagcaat ttttgttttc cctggttcgt caggacattt
     3301 tcccactgtc gtctgtcatg attgtcacga aaacgctcta tcaatccctt gcgttccaga
     3361 gaagcaataa tgcgacatat tctagaagtg gataggccta ctcgttctgc aagacttgct
     3421 gcggtcgagt gtccatctcc caaggcacag aacacaagag cttcacttac agaaagatga
     3481 taatgctctt gtatctgatc ttccaattga gcaatggaac gtagtatatc ttttaagaca
     3541 caaagttgtt gttgcataac ttgcttctag caatgattgc cacgtgcaat tatatacaaa
     3601 tatatcgata tgtcaagaac aaaacaatcg aatttcccat attttcgatg attggcctat
     3661 ccagcgtcat tatgttttcc gagtaccaac agatttgggt tatggcaaat agaccatgaa
     3721 tcatgacata aggccacgat agccggatta ttttcatcgt cgtacgctat gctctgttcc
     3781 ttaaaggaat tgaggtgata tgaagaaccg gaaaataaaa gtggatttat tgcatcaggt
     3841 tttcgtaaga ctagataagg gcgagcggat aatctcaata cttgttgccg ccaggttcaa
     3901 gaaagcaaaa tcggctgatt agagttcatt cctctatatg gaggtggagg gaattgaacc
     3961 ctcgacctta tgaatgccat tcatacgctc tagccaactg agctacaccc ccaaaaaaag
     4021 ccttcgcata ctagctcaaa tcaagtatgg gtgtcaaggc ataaatactg ctacgaacct
     4081 tgtgtataag gattggcaat attgccattc gggtcttagc catgcatact ctattcatgg
     4141 gaagttccat gcgcgccatt gcggcatgtc caatcggact agagaaagta ctagcaaatg
     4201 atcttgcgca cctgggtttt catgatgtgg gcagaagcgc aggcagggtt ttattcaatg
     4261 taagtgaaca atcgctcgct gaagatcttg caaaggcaaa tattggcttg cgcacggcgg
     4321 atcgtgtttt gctggttgta ggagaattca aagcctttga tttcgatgga ttctatcagg
     4381 gagtctcttc gatttcctgg gaacgctttt gtacgaaaga aacaaaggta atcatcgaac
     4441 gagcgcgaat taggaattgt gtgcttcatt cgcaggtgac catacagtcg atggctcaga
     4501 agtccattta tgcgtcgtta atgaagcaat ttcatatacg cacaatgccc gaaactggcg
     4561 acatactaga agttcgtatc tatggcgaca ataattggtg gcagattgtg gttgacactt
     4621 cgggagaggc tctttccagg cgtggctatc gccgtcttac tcatatggct cccctcaaag
     4681 agaccattgc tgcttcaatg ctctttctag ccggatggag tagggctcgt cctttgattg
     4741 atcctttctg tggctcaggt actattgcca tcgaggcagc cttgtatgca tgcaatgtgg
     4801 cgcccggtat gaggcgtcgc tttgcattcg agtctttttc tggcatagat aataacaaga
     4861 ttgaagatgt acgcattgct tttcgaaagc aagcgagaaa ggacgttcgc accgatataa
     4921 gggcttcaga tatcgatgaa gatgcgctcg ccttggctcg taagaatgca acgctagcgg
     4981 gcataagtga attcatacgc tttgtcaaga tagatgctcg ccagatcgag cctttcggct
     5041 caaggggagt attgctcgcc aatcctccct atggacaaag aatgggtagc gcagataagg
     5101 ctcgctccct atatgaagag cttgggccga ttattgagcg ctttctggaa gcaggatggg
     5161 aatgcggttt cttatcgtct atttcttcgt ttggggatac agtaggaatc gggcctactt
     5221 ctgtgcggga gattgtgtca ggtcaagaga aaatctactt taactggttc tcgggtacag
     5281 aagggctcaa gacttagtct tgatcaggct tctgcaatca aaagcgttcg cggatctaac
     5341 atgagtggca tatcgccccg ctctaggctt tcatgattct tgacgactcg cagatgaata
     5401 tagtcttttt taggctcgag cagaacgata actgatatat atggttcgat gtcgcgcagt
     5461 acaataggaa tacccttgga atccaataca ggctcggtat cggcaagtgt gcaggtatac
     5521 catccttcta gctgagtttc agagagaaat gcttttactt tttcgaatcg ctcgacggta
     5581 gcccttgtaa attcgaatcc atcgataatt agagtctttg cgttgaagct gccgtctgac
     5641 atgattgctt ttattgttcg tattatctga tctaccgaaa tgttttcctg catgaaattc
     5701 ataatcatgc ggttgcgtat catttgttct ttgatctcct cgatattctc gaggtttttg
     5761 cgtctggcaa tttcattgaa aatattttca taccaagaaa tgatatagct tgtatgagcg
     5821 ttgaaggaga catggatcac tttttcgccc tgcatgagtt tgtccagggc tatctgaaca
     5881 agcacggagg tctttccaat gcccttcttg cttgcaagta ttccaatatt gcccgcgtgc
     5941 aaaccgccct ctatagattt ctcaaaaatt cgcacggggc ttttttgaat gaattcttct
     6001 tttagcatgt gcgctccttt gctacataat accagacatt ataccccact tttgatgaat
     6061 aaagcgattt tacttatgcc cttttttatg cttttccagg tagtccttgc gcagttgttc
     6121 tgaaagcgcg gcaggaacgc gagcatactt ctcgaattcc atggaaaatt cggctttccc
     6181 ctgagttaaa gaacgaagta ccgtggaata gccaaacatc tcggatagag gaacctctgc
     6241 ctcaatgcga gagaaaatac tgttttcagt atttgccatt ataattccac gccgctgatt
     6301 gagcgaggcg aaaacatttc cctggaattc gtttggtgtt tcaacgacaa ccttcatgat
     6361 tggctctaga attgcaggct tcgctttatc gtaagcttca cggaatgcgc ctattgcggc
     6421 gaattggaag gcaatatccg aagagtcgac cggatggctc tggccatcgt tgatgacaca
     6481 gcgtacaccg gtaatgggaa agccgatcag cgcgcccttt tccattgcgg cgcggaaccc
     6541 tttgtcacaa gaaggaatga attcattcgg aattgcgcct ccctttatta aatcgacaaa
     6601 ttcatagcta ccttctttga atggctcgat atacccagca atacggccat actggcctga
     6661 tccgccggtc tgtttcttgt gggtatagtt gaaatcagcg cgctgtgtaa ttgtctctcg
     6721 atatgctacc tgcggcatac caggggtcac ctcacatttg tactcgcgct tcatacgttc
     6781 tatgtagact tccagatgga gttctcccat gccctgaatg atggtctgat tcgattctgg
     6841 gtcaacatag gtgcggaagg ttgggtcttc tttggtaaag cgattgagag ctttgctcat
     6901 ctggtctgag cttttgttgt ctttaggcgt tatggcgagc gatatgaccg gctcgggcac
     6961 atacatgcta gtcatggtat agtttagatc aggatgacaa aaagtgtcgc cggatgcaca
     7021 ttcgataccg aaaagagcca caatatctcc gcagttgcct tcctgtatgt cttccatcga
     7081 agaagaatgc attcgcacaa gccttcctac acgaaatcgc ttgcgagcgc gcgtgttgta
     7141 gagttcatcg cccttacgga tcatgccttg atatatgcgc acataggtta actggccata
     7201 ttggccttct tcgagtttga aggcaagcgc aacagttttc tcttggtcgt tcgcctgcag
     7261 ctcgacttct gcctctgcat tggagagatc gagagctttg ttttgtcttt ccgaaggatc
     7321 gggcaaaaaa tttatcaccc cgtccagcaa tggttgtaca cctttattcc tatatgccga
     7381 accaacaaat acaggtacta atttctcttg gatcgttcct tttctgatag ccttgatcaa
     7441 cagctctttg gttggattac catctagata cgcttccgca agctcatctg agaacatcga
     7501 agctgcttcc agaagctctt cgcggtatct ctcggcatca tcgaggagat gcatcgggat
     7561 ttccgcataa cgcagatctt cgccattagg accatcgaaa tatatagccc tcatgtcgat
     7621 caaatctatg acaccttcga atttatcttc cagcccgata ggaattgtta taaacgcggg
     7681 gtttaagccc agtttttctg cgagctgcgc cttgaccttt aagggatttg cacccatgcg
     7741 atcgcattta ttgacaaaag ccagacgggg aacatggtat cgcttaagtt gccgatcgac
     7801 agtgatagat tgagactgca cacctgctac tgaacagagc acgaggatgg ctccatccaa
     7861 tacgcggaga gatctttcca cctcgattgt gaagtcgaca tgtcctggag tatcgatgag
     7921 gttaatgcta tggtttttcc attgtacatt ggttgctcca gattgaattg tgatgcctcg
     7981 ctcgcgttcc aattccatcg aatccattgc agcgccggag ccgtccttgc ccttgatttc
     8041 gtgtatcgca tgtatttttc ggcaataaaa caaaatacgc tctgataaag tcgtttttcc
     8101 tgaatctata tgcgctgaga tgccgatatt tctcatttta tggacatcga atgccatctt
     8161 tttctatacc tcttaaatca cgaaattaag aatacaccag acaaaagaag tgtcaaagtg
     8221 gtggcagcaa gactgatttg ctgaatataa tgcaaagcat aacagatact agaaaatgag
     8281 ctaccgtttc gcaattatag aaaagatgat gaaaaagaac aagtctcagc gccaattcaa
     8341 atctcaatca gcgcacatca cgttaatcct aaggcggtgc agaggcctat atgtattcga
     8401 tttcccaagc gacttcgtgg ccatctttca aagtcgcact gactccgcaa tacttggttt
     8461 gcgagagctc gacggccttg tgaacatttt caggcttgag tccatcgctt tttttaaatt
     8521 gatagataat cttgaagctt gtgaatttct tagggtgttc ttcagcaact tttccactca
     8581 atttcatatt gaaccaggtt atgggctcgc gcatcttttt tagaatcgag atgacatcca
     8641 tgccagtgca tgctccaagc gcggcaagaa tgagtccttt tggacgggga ccgcgatctt
     8701 ttccgccact attatcgtct gcatccaaaa ctaagcgatg cccatccaac aagctttcaa
     8761 aggccatatc ttcgagccaa ctcatctgga tttctttggt catgattcct tcctctgtga
     8821 gtactttatt gatgaatatt atcaaaaaat cgaagaaatg agaaattctt ttggctcgtt
     8881 ctctgcttgg ctgggaaaag aagacggtag tagatccgac atcaattggc cataatgttg
     8941 aaaagaagat tgtcggcata gagaccagga gtggcgttag agggaacatt tcccaatgct
     9001 atactaaccg gcaacatttg gccttccatt ggcgtagttc cacagcagtt gagcttaaag
     9061 ctgtctcctt ggcgcagcgc tgggatgccg cccaatagaa gatcgtactt gaccgtgccc
     9121 tgagaatcct gattcttcaa tgcgcaacca ttcatagact gaacgactat tgagtaacct
     9181 gaggatagat tggaaatgat gtgagcggca ccaagggaga cgacggaatt agatttcagc
     9241 tcaaaacctt ttccgtttgc atccgaagta tcacctagat agccaacgag ttctgtggct
     9301 ccgcttgtac tgaagtccag agtcagattg aggatcgccg gaacatagac cataatctgt
     9361 atagaaacgc ttgtcttgct agttgactgc gcattgacgg acggaatgcc cgccaacaga
     9421 attaccgcag caatagccag cattacattt cctaaagaaa tgtctttaat ccctctcctc
     9481 ataatattat tgtagagaga gaagcagaat tagtattgcg gaaatactga aataattatt
     9541 gaattatgaa taaatcataa tagttattgg agatatttta tcgaataaac tccattatct
     9601 ccaagtggac agaaatatcg gctctaatct ctattatctc caataagcca aatcggtttc
     9661 taataaggag ataagaggcg acaaatccct tagtctcatg gatttttgat ataggccggg
     9721 gctttgctat tgtatggaaa cgagctcgat ttcgaaaacc aaaaaggagt tgggcggtat
     9781 tacatcaccg atgctctgtg caccataggc aagctctgga ggtatgatta tcattcgttt
     9841 ttctcctttt ctcatagtgc ttattacaag atcccaacca tcaattactt ctccttgccc
     9901 aatggtaaat gcaaaagggc taccagaaag cgcagattgg tcgaagacag tgccgtctag
     9961 gagcatgcct ttatagatca ctgaaacctt gctgccaggt gcaggcatag atccgaagcc
    10021 cttgcgtacg attttttgga agataccatt ttcgtcgacc tggaggtcag gccatttctg
    10081 cgcgatgagc tcgaggtcgg aatcgcgttt ttgcttattg agggcattga gtgtggcata
    10141 ggcagcgctg agtctttgat cccaagcctg ctgagaacta tcgaaggatt ttgccttagt
    10201 gcctactctt tggattgtga ttgattcgat gcgatcccct ttctgaatct gtttcacgac
    10261 atccatgcct tcgatgactt ttccaaatac agagtgctta ccatcgagcc atggagccgc
    10321 ctccagagtt atgaaaaatt ggctgccatt tgtgttaggt ccggcattgg ccatagaaac
    10381 gacgccaggg ccatcatgct ttagttcagg cacgatttcg tcggggaatc ggtacccggg
    10441 cccgcctgta ccatcaccaa caggatctcc tccctgcaca acgaaattgg caacaaccct
    10501 atgaaaagcc agcccatcat agaaatgctt gcctttggtt gcatcgagag atccttccgc
    10561 aagtccgaca aaattgccta ccgtcatggg tgctttctcg aattcgaggg ttatgactat
    10621 cgttcctcta tttgttttca taattgcata cataccatcc gggcgagccg tctgtgcgct
    10681 gcatgattga atcggaactg ctagaagtgt tagaacgaaa gcgagtggca taaagcgctg
    10741 cttcatctga aagactccta atgggttata aattttgcgt atgttcgcat cgatctgcga
    10801 tcttgcctat gatattatgc ggtataaatg gagaggtcgg caaggaggaa aagctgatgt
    10861 cggaatatcg ccgcccaaca tgggatgaat atttcatgga ggttgccaat gctattgcta
    10921 aacgagccac ttgcgatcgc ggtcgctcag gctgtgtcat agcgaagaac aaccagattc
    10981 tggctacagg atatgtgggt gcgccttctg gattgccgca ttgcgatgat gtgggtcatc
    11041 agatgagaaa gatgctgcat gaggatggca gcataaccga acactgtgtc cgaaccgtgc
    11101 acgcagagca gaatgctatc tgccaggcag cgaaaaaagg tgttgccatc gaaggagcaa
    11161 ctctctattg ccgcatgact ccatgccgga catgtgcaat gctcataata aactgcggca
    11221 ttgtcagagt tgtcgctgaa tatcgctatc atgatgcaga ggaaagcgag gcaatgttca
    11281 aaatggctgg catcgtactc gaatatgtgc atagagaggt tcttcaatac tcaagacaat
    11341 gaatgaaaga aggccggtca aagggctcta ttttttgaaa tattcgcaga agcccgttgt
    11401 agaaatagcg aattcccgct agcatagcac attgtgggaa taacgattgt atttcttggt
    11461 gagattgtcg gaaagaccgg tgtattcacc gtaaagagca ctatgcctga gatcaggcag
    11521 cgcttcgagc ccgactttat ctttgcaaat gcaaactctg caacgggatg tgcgggcttg
    11581 ggtatccagc atgcggtcta tcttcgcaaa ttggggatcg attgcataac tatgggtgag
    11641 gctgcttatt ataagccaga catgactgaa ttctacccta aagcaggatg ggttctaagg
    11701 ccggctaatc ttcccgaggg ggatcccgga cgcgggtgga gggttttcga gaaaaatggc
    11761 aaaagaatgg cggttgtcat gcttcttggg cagtctggat ttgcaaggat ccatgcagac
    11821 aatccctttt tggcaataga tcggctcagc aatttcctgc gcaaggagac cgcctgtatc
    11881 accgtcaatt ttcacgctgc gactaccgct gagaagctct ctatggcaag gcacacggat
    11941 ggaaaagtct ccgcaatgct tggttcggga ggcaaggtgc tcacggccga tgctcgcatt
    12001 ctcagcaaaa aaacggctgc tattaccgat ctgggcagaa ctggcagcat gctatctgta
    12061 ggaggccttg atccggatgc taaggtcaag gaatttttga ccggtatacc gtcatggtca
    12121 agagaagcca gcgctcagcc ggaagcacaa ggaacatata tctgcttcga tgatgacggg
    12181 aaggcgattt cgattgaacc attccgcatt tccggcaagg agatcgtaga tgagggagat
    12241 agcaatagta aaaaaaatta agggagacat agtaaccgtt tcaatcgaga tgcaagaagg
    12301 ttgtgctgtg tgtgtcaaca atggtagatg caagatacgc aagagtgatc tttccgtgta
    12361 taacaaggat catatcgata tagatgaggg agatcgggta gtagtcgaga ttcgcacaag
    12421 cgaacaagca aagagcgctt tttgggtcct gggttttcct cttatcatgc tttttgttgg
    12481 ttatggtttt ggagctttta tcttcaaggg ttcgggcgaa ggacctgccg tggtttgcgc
    12541 cggcttgggt tttatctttg ccatgggaat aggaatgctc attcaatatc gcagaaagct
    12601 aaaatccttt ccttatattc taggaaaaga gggcgaaatt ctctagagaa gcgataatcg
    12661 atgaatccga acgataggcg aatccacttt tagattttct tctaattttc ttctgatttt
    12721 ctgttgataa aggtgtgcca tgattttttg taaggcgtat gcgctatgat ttcttgaaag
    12781 ggcaatcgct ctttattgat gtaatgcatg ataattttat gcggaggtgg gttcacgaga
    12841 attttgcgct aagataatca ggcagcatat cttagagccc aataattcta atcgcggttt
    12901 atctattgac aagaagagac aaaatcctct attgttctgt cttaaaggaa cacttatgat
    12961 tcagacacaa gatctttcat gtttcatgca aagcggccag aggaggccgc tgatcaggcg
    13021 ctggtagcgc atctttcccg ccagacctcg catcgcgggg ggtctataag ccctaaaggg
    13081 gcctttccca aagacattga tggttctcat acaaagaaaa ccgagctggc gatctacatc
    13141 gatgaagtgt cgaagtcttt tggcaaccat agcgtgctgc acaaggtatc gctcgcagtg
    13201 cccaggggaa gcatccatgg cattatgggc aagagtggag cgggcaaaac taccttgatc
    13261 cgcattgctg gtcttctgga aaagccagac cagggctcaa tttggtatga aggtatcgga
    13321 atgcctgtgc atgagctgca tgggcgtgca atgctcgagg cgcgccggca ggttggcttt
    13381 gtgtttcaaa gctttaatct cttttcttcg cgaagcgcat cgggcaatat agcctttcct
    13441 ctcgaagccg cgggttggcc tcgagaaaaa atccgtgcgc gagtagagga acttcttgag
    13501 ttcgtcgagc tttcggacaa ggctaacagg cctgctggca ggctctctgg cggcgaaaag
    13561 cagagggtgg caatagctcg cgcgctcgca aatagccctt cgatgctttt gagcgatgaa
    13621 gcaacgagcg cgctcgatcc ggagacgaca agagcggtgc tagatttgat tcggtctctg
    13681 cgagacagac taaacctcac cgtaatcatg gttacccatc agaaagaggt catacggcgc
    13741 atctgcgata ccgtatcggt gatctccggc ggatctatcg tcgaacaggg ctctgtggcg
    13801 gaagtcttct ccaatcctcg ttccgaagcg gcaaaggctt ttctcaagga agaagaagaa
    13861 aaggggcaca gctatgtctg agatacttcg actccttatt cgacccttag gagagaccat
    13921 cctgatgata gccgcttcga cattgatatc gactatactt ggtggcggat tgggaatatt
    13981 gctatttact ctttcgcaag atcgctttgc gggtaagtat cctggagctc tcttagtaaa
    14041 gcgtatagct gatcgtgcaa taaatatgtt ccgctctttt ccatatgtca tccttatggt
    14101 gctcgtcttg ccgctgtccc gcctgatagt tggaacaagc cttggaacta tcgcgtccat
    14161 tgtgcctctg tctatagctg caattccttt tctagcgcgc attgtggaat catcgctctc
    14221 cgaagtagat tcgggagtaa tcgatgcagc aatttcttgt gcagcctcaa agacgcgcat
    14281 cctatttgcc attctaattc ctgaggcgct accatcgctt atatccggcc taacactaac
    14341 gatgatcagt ttgctcggtt attcagccat ggcaggtgtt ataggaggtg gtgggcttgg
    14401 agatctggct attagatacg gctatcaacg ctttcgcgcg gatgtcatgg ctgtagcggt
    14461 agtggctatc gttgcgctgg tggaacttat ccaatggacc ggttcaatga ttataaaacg
    14521 aatcaaggcg gcgcgctgat agggtagctg ggttccaaga agcgcgcgag agagataaag
    14581 cgagcatcga acttattttc gagtattgcg cgagcgaggt cgaagacatg agcattggtc
    14641 cgcccaatta tggccgagtc agccgtagct atgagctcac cttcccaata tgccagaaag
    14701 tgatcgactc cattttccaa gcgaacttcg gcattgatag accgctgagc aaaggcttct
    14761 cgcaggaatc cagcctgtcg accgctcgaa gagaccggag cgtcgaggaa gaaagtcaga
    14821 ctctggagat tgagggcggc caggtattct gccgtaattt ccgccatacg ctcgaaacca
    14881 cggtgatccg ctatgcgccc atgcgcgccg cccacatcgc gcaagaatcc gtccgtggca
    14941 atgaacatag ggtgaccatg catatagttc atgagagtga aaaggatatt gtaaccatcg
    15001 acaccgagcg aagctctctc ccatggactg cggacaagct tggcgagatt gatctgggaa
    15061 gcttttagag aaaggatacc tctatatagc atcatgcgcc cattgtgcga aagcctatag
    15121 cgatccccta ctaatttgag gcttgcatcg atgggatagt ccttatcgag tagcattcga
    15181 taatcccttg cagcggcaat aaatgtatca tcaaggactt cagtattctc tttcttttcc
    15241 atagaactcg ctctaaagga tccaaaggac ctaaatggtc taaaggagct tcttgctcag
    15301 gtcgatttat gaccaagcct gacttattaa gcatatacta cactctttgg cctgtcagcc
    15361 tgctttctgc ccaagcgctt atttatgccg atgatctttc gccttccttg ccggttttgc
    15421 ggcgctctgt tattgtttat tttatttaca tgcctattaa aatatgaagg agaagctatc
    15481 gatgattcat gaaagcagct ctatctatca gatatttttg cgtaatttta ctaaagaagg
    15541 aactttttgc gcagctatac cagagctcaa gcgcatcgcc gacatgggtt ttgaatggat
    15601 atatctcacg cctatacatc ctataggtga aaaggctcgc aaggggactc ttggcagtcc
    15661 ctatgccata aaagactatc gtaagatcaa tcctgagctg ggagacgaag atgattttgc
    15721 tgcctttgta caagaggtgc atgctcatgg gctaaagctc atgatagatg ttgtattcaa
    15781 tcatacttca cccgattcgg tgcttgtgca cgagcatccg gactggtttc ttagagccgc
    15841 ggatgggctt ccatggagaa aatgcgaaaa ttggagcgat gtggtagatt ttgattacag
    15901 gagctcgcct catctctggc ttgagctaat cgacacactg acaatttgga gagatagggg
    15961 agtggacggt tttcgttgtg atgtggcatc gcttgtcccg agcgagtttt ggagacaggc
    16021 tcgacaacgc gtaaatcaat acgatcccgg aattaaggag gagagagccc agcttctttg
    16081 gctcgcggag agcgttcatc cagctttctt aaggaaaatg cgattgcttg ggtacaattc
    16141 atggagtgag cctgagttgc atgtagctgc tttcgacctg acttatgatt acgatggttg
    16201 ggaaaggctt gagaaaatat gggagcaagg gatgcccttt gagcgatact tggaatatct
    16261 ctacgttcag gagaccctct atccaaaagg ggcaaaaaag ctcaggtttc ttgaaaacca
    16321 tgatcaagag cgtgcggctc atagatttgg aaatggagcg aagctgcgcg caatgactcc
    16381 tttttatcaa ttcttgcctg gagtagcgat gagctatatg gggcaggaat tggctttaga
    16441 gcatatgccc agtcttttcg aaaaggatcc tatagattta gagcgtggag ataaagagtt
    16501 cgagcgctat tttattgcga gcctgcgggc tacaaaagaa gcaaaaaagg aagccccttt
    16561 tttttcatgg ttaattaagg gtgatgccgc gcttcaagat attactgacc aagacatggc
    16621 cagccgagat aaagcgccga gaacccgaac cgtccagaac cgcacatcga acaattgcgc
    16681 cgtaagagaa gaagtcatct attgtctaag gagtgtcgaa cccgcaataa gtccttattt
    16741 gatcgatcga tccatgataa taaaagggaa ttatcttctt gtgatacagc ccgaattgga
    16801 tgcttctggg ccaaaatcca gcggtgttaa atctcaaagg agaatttcgt ttgcagagga
    16861 atctatgcct cgcgctctct ttgacatcga aggaacggat ctgctttctg gaaaagaagt
    16921 gcgaattgcc gagggcgaca tactaccaag aatacccgcc agcctcattc gcctaaagga
    16981 ttgatactcc aaatatgtct cattgtgaaa gaatcattgt ggtatttccg agacactctc
    17041 gagcacaaaa gtgggtgcat aggcgaattt aggcatatct ttctcatcgg taacgcctgt
    17101 taggacgagt atggtttcta tttgagactc tatgcctgct attatgtcgg tatccattct
    17161 gtcgcctaca ataattgttt cctcatatgt tgttcccaga gttcggagcg catgcctcat
    17221 catgagcgga ttgggtttgc cgacaaagta tgcctttttc tttcggatat gatccaaagt
    17281 ctcttttgtt gctttcattt cttccgtacc tgcaattttg atattactat caaagacaac
    17341 atcatggaat cgattggccg atagacttcc ctttcgatcg cctcacctga aggatttcct
    17401 gcttttcttc aaggtttcga accgtctttt tggtcatctt atgaagtttc tcctgtgcat
    17461 ataaaagcgg atcttccgga cgtcgagaga gagaaaggtc catccaggtg ccatcatttt
    17521 tgagcctgta ggcatgtgtg ttgtctgcaa aatagatatt caagatgtcc ttacatcgtg
    17581 ctttgatttc cttatctagc accgggaaaa gcaactccag tcttttcttc atgtttcgct
    17641 ggagccagtc ggcgctggag atatatagtt cttccgagcc accgtttcgg aaatacagca
    17701 ttcggccatg ctcgagatag cgtcctaaga ccgagcagac ctcgatgttt tcggaaagcc
    17761 ctgccatccc ggggatcagc gtacaaaccc ccctcacatt gagctttatc tgtactccct
    17821 tggcagaagc ccggtagaga gccgctatca agtcagggtc cgataaagaa ttgagctttg
    17881 caattataaa acccggcgat tccggagtgg tttgggcggc ttctctttct ataaatccga
    17941 tgatgcggtc tttgagatcg aaggggctaa gggcgattag agagagcgat tgtatgttcg
    18001 aatagccagt tagaatgttg aaaaaaagag caatgtccgt gcatagacta gggtttgcgg
    18061 tgaatagaca gaaatcgaca taatttcgcg cggtcttttc gttgtaattg ccggtcgata
    18121 ggtgaatata tcgcgcaata cttccatcct gtcgttttct gatgatcaaa gctgcttttg
    18181 catgtacctt aagccgagcg actccgtagg taacgattgc tcctgcctgt tcaagctcgg
    18241 tagcccatga gatattcttt tcttcatcga atcgcgcctt gagctcgagg agaacaacaa
    18301 cttgctttcc acttcgcgcg gcctttgcaa gggcatgcac aataggcgaa ttcatgccac
    18361 ttgttctata gagtgtcatt tttattccca gaaccgatgg atcgctagcg ctttcttcta
    18421 tgaagcgctg gaccacatcg aaggattcat aaggcagatt tacgaatcgg tcatgttcat
    18481 cgatccaatc gaagatgctg cccttttctt cgaatagctg aacaggtttc cagggctgga
    18541 agaatagcac tctatccggt gtaaagccat gagccttgta gaattctgga ttgcagagct
    18601 catcgactct tcccaggtct ataagccagt cggcctgata tagatccagg gcatcgagcg
    18661 agaggctttg cctcagggca tctatgagaa cctgatcttc tccggaataa accatccgca
    18721 caggcgtaga attttgccgc ccggtcaata cttcctccat ggcggtcaag aaatcatcat
    18781 ccctatcttc atccacgctg aaatctgcat ctctgtttac cttgaatata aggccactcg
    18841 atatttttag gccggcaaag agtttacttc caaatgccag aatcacatct tccaggagag
    18901 caacgcgaaa ggctgcaata accatcgaat ttgcagaatt atccggctca agacttagat
    18961 attgaggcag aatgatgaaa cggtcgatat tcttgggcac ttgaataata gccacagagt
    19021 cattctcttc gagtctaaat gcgacatgga cctgaagact gcctgtcgat gggaaatggt
    19081 tatcttctag ccgcaaggga gtaaggagag gatatatcct ttccgcaaaa agcctttcga
    19141 gatatctgct ttctttggtt gtccagtctt tttcttgaac aaattgaata ccgtgttgaa
    19201 ccagactggg gagaatcgtg ccatgcaaat gcatatggag cttatcatag ctggcttgaa
    19261 ctttctcatg aatagcctcc agaagcgcac gaggctccat accagcccat tcttcattcg
    19321 tctggcctgt ctgccccgtc tgcacggctg tcttgacggc agcgacacga accatgaaga
    19381 attcatccag attggatgca aaaatacaca taaaattgag ctgttcgagg agaggcaaag
    19441 agcctttgag tccttcctgc aaaacacggg cattgtaatc cagccacgaa agctcacggt
    19501 ttagcatcgg aagttctttc atactcttct ctatttgtgc tcacatcaat atgtcttaga
    19561 ttaaaacagg tatgagccca aagacatctt cgaacatgtc gcctttctcc gcgagagaga
    19621 gtttttccaa cgagaaatcc agcgactccg aagttcgaat tataaaccta tcctccgttc
    19681 gttcaaaaga cagctctttg atacggccca ggtgacttcg atcgagcgca tccgcgacgc
    19741 gcagaatggc tgccaatttg agcactatta tgcggctttc tcgcggaagg gcgatgaaat
    19801 tgacgtgggt cgaaagcggc tgagattttc gatgatagcg aactacatta gatactatgt
    19861 tgatatcatc catattgaga ccgaagatct cggagttggc aactatatat tcgctgtgtt
    19921 tatggtgtcc acttgttctt aggtaagtcc ctatatcatg gagaatcgct gagatttcga
    19981 gcaacattct ctcatgaatg ccaaggccat gttccttttt tagggcatcg aatatcttaa
    20041 gactgatgtt ccttacatga gcggcatgag cctcatcgta atggaagcgt ttgccaagag
    20101 cacgagccga tgctactacc tgtctgcgta gttcctcttc gattttttta ttcattccct
    20161 ggacctgcgc aagcagaatt ccttcgcgaa tagagatatt gggtacgata atcgtatctg
    20221 ctcctgttct ttcaagaaac atgcgttcaa tggtgagccc cgcggtatag ttttcgactt
    20281 cattccatga aaggtgatat tgtgccatgc attcctcggt ggacatgttg gcaacatagt
    20341 ctgcaaattc gatgaactgt ttgcggtcca gtaccgcgta gttgcccagg actttttctg
    20401 aaaggcagtt tgcggcaaag cgcgcgtccg caccaataag aacgaatatg cgcaaagaat
    20461 cgagctgcaa atcttcggcc aagatatcgc aggctgttct tatattgctt tcgagataca
    20521 tttctatgaa ttgcctgggt tgctgtgctg attcgcggat ctgttcatct attctaagag
    20581 tgcctatgct aaggc
//
LOCUS       DAOOWY010000026        20093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2682_length_20093_cov_12.979090, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000026 DAOOWY010000000
VERSION     DAOOWY010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 20093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20093
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2682_length_20093_cov_12.979090"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PK105_03545"
     CDS             <1..295
                     /locus_tag="PK105_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase"
                     /protein_id="HOU60813.1"
                     /translation="TANASQGRLRGRTDEALVLTGKDKWYVRAAKLGRLYVPYDENGH
                     PIEERVGRHVTAIAEIINSWNWEHPETPLEFDNIPSYEDLLSKGLGEYLLPVQ"
     gene            complement(577..1557)
                     /locus_tag="PK105_03550"
     CDS             complement(577..1557)
                     /locus_tag="PK105_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU60814.1"
                     /translation="MIYRHLGSAGIKVSVLSLGSWVTYGSQIDIDSASQMIKYAYDHG
                     VNYFDNAESYAGGQSEVIMGQAFRRLGLRRGSYLVSTKLYWGLHEGPNEKNTLNRKYL
                     LEAIEGSLERLGMKYVDLLFCHRPDPETPIEETVWAMHDIISSGKALYWGTSEWSAEQ
                     IRQAWETADKHNLRKPQMEQPQYNMLVRTKVEKEFARLYQGIGLGLTTFSPLASGLLS
                     GKYKEGIPKDSRFALYGYEWLQERWYQPEIISSIERLRPIAESIGSTMSQLAIAWAAR
                     NPNVSTVITGASRLSQLEENLGAIEILPRLNAEILEKIDEALEPILVMRN"
     gene            1787..3061
                     /locus_tag="PK105_03555"
     CDS             1787..3061
                     /locus_tag="PK105_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOU60815.1"
                     /translation="MSNSDRIFKIVHISDLHLGKTLRSQDLAQDQSSILKQIVEKVKM
                     LKPDLVIIAGDVFDRSIPSESAQTMFGWFMAELRRALPKDGRILVIPGNHDSPKRIAF
                     AAELFESVGIHIVSEILSNPTLVLKKDGKRAAVWALPFVTHGAYHEFKRNLTKTGYAE
                     MASAEANSSMAGRMESIIANLRPSKSASESEYDLNILVAHCYVKEAVLSESDIAFIGG
                     TEAVPASLFESFDYVALGHLHKMQALSPKVWYSGAPMSMSFGDGDIEKGFLYVELEDG
                     KPARVQRMELKPLHVFKRVRGSFDELAAESALIDDDYVEVILTDEEPVYDAFNRLAIR
                     FPNLMTVRQEAFEKRIGTEVEEVGIPVPHIVSDGETENADALEDFSGKSLHATVLKDF
                     VGFTQRILDEPPSEELLNAFEVLLRNAEEDLL"
     gene            3058..6606
                     /locus_tag="PK105_03560"
     CDS             3058..6606
                     /locus_tag="PK105_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU60816.1"
                     /translation="MKPLYLSLENFGPYRQRQDIDFCKLDDFFLIYGKTGSGKTTIFD
                     AITYALYGKAIGGRSNLERELGSRFSPIGSKPWVEFEFLASSAQWKVYRSLPYKKRNK
                     RGVESEATSEVELYRMNEIGVYEIIADRSTTANQILLDLLRLSADEFSKIVLLPQGEF
                     QKFLEMKTTERVEILEKLFDVGIYDAVTEAARKKVMELEAYLKAKQEEIERISNELGI
                     EPGSRIKESGKAISEFDKRIEESTKDASQLETQIAQNRERLSFLQSMLAAWKNLHEKE
                     ELKPVFDKRETRLEAAKSLGELASLAKQVSADYEELIELLSESLQTGTELSKLEAEGI
                     EIKKIQKGLPIVRKKYDNLQRSVALYQNAVNAWNQKEQCESERRQVMQRLDATLQDWE
                     YRQNEIGQLKSSITEIENAIQSEPLAQESFHKIVIADEAFKRIETWIEQKEKIEAQRV
                     ETLRKIREAEDSILLTNQELTIAERNRDRINEEIRKAQAGMLAASLIPGEPCPVCGAR
                     DHPNLAKLEQNAPDDKTIKTAQKAVEETKSSLSAIIQDKQNLENRIEEFSQDLQVIVQ
                     KISEEWKGIACEVIGDKSTMQLLDISPSEFVSISSRLQSERRELEERLKSYQHGRKSL
                     QELRKTLDQAGRDLDRIQEEKTRLEMKQTELDAHIDSLGEQIGKEDPRICLKEAEENL
                     VTLHNEIEQIEEQAQKWEIAWSSISIKMDMQLEKIGQKGPALATMVEEFLNEVDKMKV
                     RSLLRLLESPVTSSEKEAPEHEQQAIRPLPDFLTSSGDNAGNLSGCSESIYEPSTRLL
                     QELFALLANSLPHIDRFVPVSGQIMYLSETLDKILSAHRINEPALKALMQEILCSVWT
                     SNRIKEEAREISAFRQSYNEASISYQVLAARAQALSPFNSLSPNSFNSTTLPSSNQSP
                     DRLPQHNQENGDQAKHPEMFDGIHLEQQEKNLREECLSLLERKKSIETEISDLRDERA
                     RFIAELDQLRRLLSRRDELTSEYERAIKEFGKHQELSKLLGGSLNPQRKMPFKNYVLG
                     LQFREIAMRASSRLYRMSNGRFLVEADILGGSGNQKIGLELYVLDAWNGGRRPVGTLS
                     GGEKFMLSISLALGLADSIQERARANRIESLFIDEGFGSLDEESLSLAISVLEDLRGD
                     KSIAIISHVDELYSRIPSRIVVKKSVGGSHLELEQD"
     gene            complement(6619..7791)
                     /locus_tag="PK105_03565"
     CDS             complement(6619..7791)
                     /locus_tag="PK105_03565"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU60817.1"
                     /translation="MYEFYAPTKVLFGDGCLAEASPRLKEMRATNVLIVSGRSKTASL
                     QGFRELCIGLDASGIRYVHFGEVSADPDTSLVDKGTEVYRAQHCDAIIGFGGGSPIDC
                     AKGIAASVAEGRSIRDFVGTGLAFTKPVPPLIAIPTTAGTGTEVTNAAVFTVVDESGR
                     RSKKGTSSQFYFPKLAIVDPVLHLSMPPSLTAATGMDALTHAIEAYVSRFNTPISDMY
                     CLESIRLIGRSLRSACHISEGAKAMQSRRDMALSATLAGVALSEAGLGMVHGFAHAVG
                     ALAGIPHGLANAIILPFVMQSLIPDAKGRLSDIGQALTAKDNISPEESVRAICQLGHD
                     IGIPENLEAAGVSPSYFEDILSDALSYRRRKASPRAFTDEELRELLERAFHGAPQQ"
     gene            8036..9586
                     /locus_tag="PK105_03570"
     CDS             8036..9586
                     /locus_tag="PK105_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU60818.1"
                     /translation="MKKILFALIALVAVLGLSAQDFTIVNGTEPTSLDPHAVEGVPEH
                     RIYVALFEGLTINDPRTNRALPAVAESWSFSKDYKTCTFKIRKGVTWSDGVEITADTV
                     VQSWVRKMDPQNAFQYADLPAMYIEGGSDYLEGKAGPESLKIRAVDKYTFEVKLVGPI
                     PFFADMVSHYAFAIVPMHAIEKYGKDWVKPGKMVSNGPYVLSEWKPQEKVVVVKNNKY
                     WDAKNVSLKKVTFIANDDINVGYNLYKSGAADWTEAVPLELMDEVKLRKDFHVAPQYG
                     TYYYIYNTTRKPLDDPRVRKALAMAINKDDLVNKVTRGGQIPANSLVPPSTGYTPAKG
                     YNFDPAAARKLLAEAGYPDGKNFPAFTILYNTSSSHKSIAEFIQAQWKVNLGIDVSLM
                     NQEWGTYLDTRSQSHNFDIARAGWIGDYLDPSTFLEMFITGGTQNDGLYTNPKYDELL
                     DKARVASGTERFQYLMDAEKILIDEDMAVLPLYFYVTQNLIDLDKWDGWYANPLNIHP
                     WKFIRPKK"
     gene            9813..10739
                     /locus_tag="PK105_03575"
     CDS             9813..10739
                     /locus_tag="PK105_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60819.1"
                     /translation="MPRYFIRRILSIIPTLFIVITLSFFLIRLAPGGPFAREREVPEA
                     ILQNLMKRYHMDEPLVKQYLRYMGDVIRWDFGPSYRYRDLSVNEIIDEGLPISMSLGV
                     ISLVLATVGGIAIGIISALKQNRWQDYVVTSIAVIGISVPLFVMGPVLQLVFGMKLKI
                     LPIGQWISTHGIKAVILPAITLSFPYFAYIARLSRASILEVLRSDYIRTARAKGLKES
                     VVVWKHVLKGAMLPVVTYLGPAFSGIVVGSIVVESVFLVPGIGRPFVQSALNRDYTLI
                     MAEVVVYSIILIIANLVVDILYGFLDPRISYK"
     gene            10750..11988
                     /locus_tag="PK105_03580"
     CDS             10750..11988
                     /locus_tag="PK105_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60820.1"
                     /translation="MAKKQLETAVNEFNQVMKPVSLWADAWKRLRKNRMALISLVIVC
                     IYLVISIFAPLLTSAGILIDYRKQVITNASLPPSFKPAGELVINKIEKRIADLEQRVE
                     EQKQQQSAFDSFFDFGSVYAQDENGEAGDDFAPSQEEFSFTYEGEGFFGNTTDPVVRE
                     LISQKETLEHVKAELDTNPDYKIVYILGTDNLGRDMFARIIYGGRISIAIGVVGTLTA
                     ALVGILVGAISGYVGGWLDNFLMRFVDIMYGLPYMLIVIIIMAMIGEKARGSFVVLFV
                     AIALVSWLTIARVVRGQIISLKNSEFVEAARSMGASTQRIIFRHLLPNTLGVIIVFST
                     LMMPSFIMNESFLSFLGLGVSAPDASWGTLVSEGVRAMESYAWQLLGPGITMTIFLFC
                     MNFLGDGLRDALDPQSKNRT"
     gene            12001..12993
                     /locus_tag="PK105_03585"
     CDS             12001..12993
                     /locus_tag="PK105_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60821.1"
                     /translation="MSDEVILQVRDLKTYFTVDEGIVKAVDGVSFELHRSETLGIVGE
                     SGSGKSVTNLSVINLIPTPPGKIVGGEVLFHGTDLLKLPPGEIRHIRGNKISMIFQDP
                     MTSLNPFLRISTQMIETIQLHQGLDKKAAKEKAIEMLRLAGIPAPEKRIDQYPHQFSG
                     GMRQRVMIAMALSCNPEILIADEPTSALDVTIQAQILDLMKELTRKLGTAVILITHSL
                     GVVAGMCDTICVMYAGRIVERGRTEDIFENPKHPYTIGLIRSVPRLDQETKMRLYSIP
                     GQPPNVIDLPDCCPFYPRCEQSMDICRGKYPSIVSFENNQSVACWLYNKETQNA"
     gene            12986..13993
                     /locus_tag="PK105_03590"
     CDS             12986..13993
                     /locus_tag="PK105_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU60822.1"
                     /translation="MPETDEVLLDVRGLKMHFPVKTSMFSKTKNYIYAVDGIDFKVRK
                     GETLGLVGESGCGKSTTARAIDQLYKPTAGEVILKGKDLTKLPPSEMMEARKNIQMVF
                     QDPYASLNPRMTAGDIIAEPIRIFQKQGLIEMSAQDRNNRVEELMEKVGLSRYFKNRY
                     PHEFSGGQRQRIGIARALALNPELILCDEPVSALDVSIQSQILNLFKDLQGEFGLTYL
                     FIAHDLSVIKYISNRVAVMYLGLIVEIADAADLYKKPLHPYTEALLSAAPIPDPKIEA
                     KRKRIILTGDVPSPDKQRPGCNFYDRCPKRMDICKERRPSLKDSEVGHAVSCFIFHKP
                     E"
     gene            14520..15317
                     /locus_tag="PK105_03595"
     CDS             14520..15317
                     /locus_tag="PK105_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU60823.1"
                     /translation="MNRKIVTFIFLASLVSTMLVFAGPFSSGTLRVGATSVPHAEILN
                     FIIPDLAAQGIKLEVIEFSDYVTPNIALAEKQLDANFFQHLPYLETFAAERKLSLESV
                     IAVHVEPLGLYSKKYKKVSDFPSGSIIAIPNDPTNEGRALLFLQSKGIIKLKPDAGLQ
                     ATIRDIVENPKKFVFKEIEAPQLPRTLDDVAGSIINGNYALQSGFNPIKDSLLLEGGE
                     SPYANILVVRKGDSNDPRIQALAKALTSQRVKDFILNTYKGGVIPAF"
     gene            complement(15403..16347)
                     /locus_tag="PK105_03600"
     CDS             complement(15403..16347)
                     /locus_tag="PK105_03600"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HOU60824.1"
                     /translation="MYWDDLIAQVGNLALTATLYEVSAAPKPGLVDPFSRGAHSDMDY
                     FTFLASAAALAPFFVDFARIGAEFAGESPGLLAELRKLGIEAEKAMYAATQGINTHKG
                     LIFSMGLACAAAGKLLVSDRRAEISDRSAVISGCIQEVADIVSGITIRELVPLVEAEM
                     LLASDTIRAKNAVVPSIDLSAGEQSFIKYGIRGVRGEAEDGFPSVINHALPRLHFDLL
                     AGMSWNDAMVDALLILYLKVDDTTVLKRAGSDGLAYVRAQAAAALSRGGMRLMENRDF
                     VQKLDADFSQRGISPGGCADLLAVTAFLEMLSNKWIAQ"
     gene            complement(16392..16940)
                     /locus_tag="PK105_03605"
     CDS             complement(16392..16940)
                     /locus_tag="PK105_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HOU60825.1"
                     /translation="MLSLQTVLAARELRWKEEELLAKSLAQEFSGHRLAFAFCTLRMP
                     ASLRIYGPYKEIAVQLRDALAEALMEKAFTIIASGQKQYDDGPSAWLASCCDEEILKR
                     MAISIEEGHVLGALGDIDVTDAEARLLSRRDMGFEPRKCLVCDNEAMVCSAGQCHSLL
                     DIAAHIEAIISDSMALRSSKSL"
     gene            complement(16934..17203)
                     /gene="citD"
                     /locus_tag="PK105_03610"
     CDS             complement(16934..17203)
                     /gene="citD"
                     /locus_tag="PK105_03610"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HOU60826.1"
                     /translation="MRVLKSVTAGSLESSDVLVTITPVSEDHIEYDIESIVIKQYGKR
                     IRQITEEMAESAGITGARIHVQDRGALECTLRARLETAIERSIVC"
     gene            complement(17247..18404)
                     /gene="citC"
                     /locus_tag="PK105_03615"
     CDS             complement(17247..18404)
                     /gene="citC"
                     /locus_tag="PK105_03615"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HOU60827.1"
                     /translation="MPRVEGYRTGRLENIIGKHMSELNFGLEERILNSKDELAQREAL
                     LTSRGLMVPEGEELVLGLFVGEQLVATGALVRNVLQGIAVDIDYEGEGISAAIVSSLI
                     RRAVDRGITQVFLYTISEDISRFESLGFRKVAAVPRGAALLEWGTAGIESYLASIRAL
                     AKDKPDCAGAVVINCNPFTLGHRYLIEYASSHVPWLYVLVVEEDRSLFPFKVRYKLVQ
                     EGVADLRNVTVMKGGPYIISSATFPTYFIRPDVNKASWKAVRMHASLDVSIFRTHIAP
                     ALKVTDRFVGTEPYCPTTSIYNDIMKIILQAKETEAFGLDAKTPPVALHEIPRFELWG
                     TPVSASKVRDMIREGNLSEVQKFVPGTTWDWLKSEEAAPVIERIRQSDSRH"
     gene            complement(18373..>20093)
                     /locus_tag="PK105_03620"
     CDS             complement(18373..>20093)
                     /locus_tag="PK105_03620"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOU60828.1"
                     /translation="FENADDHNFLQDIASRYGIVFSRPGNGICHQVHLERFGVPGKSL
                     LGSDSHTPTAGALGMLAIGAGGLDVALAMAGLPFSIPRPRVLKVELRGRLSPMVSAKD
                     IVLEVLRRLTVKGGVGKILEYAGEGVSTLSIPQRGTIANMGAELGATTSVFPSDEVTK
                     RFLEAQGRAEDFIPLFADSDASYDEEMIVDLNTLEPMIALPHMPDNVVPVHEIAGLPV
                     DQCFIGSCTNSSYYDLAVSASILRGHHVAPQTSLVVAPGSRQVLLMTARDGILTSFVE
                     AGARILECACGPCIGIGQAPATNAVSLRTSNRNFEGRSGTKSARLYLASAETAAASAI
                     AGVITDPRTVKSRVTISIPESFINDDSMFIFPSTEGEKAGIRRGPNIAPLPQFEAIGD
                     ELRAEVLMHMGNNITTDHILPAGSRIMSLRSNLPAISEYCFSVLDADFPARARAAGSG
                     ILVAGQNYGQGSSREHAALSPRFLGIRAVIAVSYARIHRQNLVNAGILPLIFDSSEEI
                     QNISQGDMLYIKNIHEGLRLDRFEVKNESRGTTFMAHHGLSQRQIEIILAGGALNAAR
                     RRLSNR"
BASE COUNT         5467 a         4897 c         4604 g         5125 t
ORIGIN      
        1 gaccgcaaac gcttcacaag gacgattgcg tgggcgtaca gacgaagctc ttgtgttgac
       61 cggcaaggat aaatggtatg tgcgcgctgc aaagctcggg cgcctctatg ttccatacga
      121 tgagaatggc catcctattg aagaacgggt gggaagacat gttacggcaa tagccgagat
      181 tatcaattcc tggaattggg agcatccgga aacacccttg gaattcgaca atattcccag
      241 ctatgaggat ttgcttagta aggggcttgg agaatatctg ctgcctgtgc aataagaccc
      301 aaaaatggag ctaaactaag cgccctactt ctgagttatg agtttctcga tgtgtctgcg
      361 gtgtttttag ggcggtgcgc attttagctg tggacataga aatttgtcgc attatgtagc
      421 tcgcggcacc aaaaaacaga aaactctgag caccgaacaa aatgcgcacc aaattcccct
      481 atctcccaaa ctcccggcgg caatgaagct catcaacgag cgccgaacaa gatgaacgct
      541 aaatctccct atctcgctgg atcctcgaat tcttggctaa ttcctcatta caagaatagg
      601 ttccagcgct tcatcgatct tttctaggat ttcagcgttg agtctgggca gaatttcgat
      661 agctccgagg ttttcctcga gctgggaaag ccggctagct cctgttatca ctgtacttac
      721 attggggttg cgtgcggccc aagctatggc aagctggctc atggttgaac cgattgattc
      781 ggcgataggg cgaaggcgtt caatgcttga tattatttct ggctgatacc aacgctcttg
      841 aagccactca tagccataca gcgcaaaacg cgaatccttg ggaatacctt ccttgtactt
      901 gccggacaga aggcctgaag caagagggct aaaagtagtg agccctaggc ctatcccctg
      961 gtaaaggcgc gcaaactcct tttcgacctt ggtacgcacc aacatgttgt attggggctg
     1021 ttccatctgg ggttttcgga ggttgtgctt gtccgcagtc tcccatgctt ggcgaatctg
     1081 ttcggcagac cattcgcttg ttccccaata gagagctttt cccgaggata tgatatcgtg
     1141 catagcccag accgtttcct ctatcggcgt ttctgggtcc ggacgatggc agaacaggag
     1201 gtcgacatat ttcatcccca gacgctcgag gctgccttcg attgcctcga gaaggtactt
     1261 gcgattgagg gtgttctttt catttgggcc ttcatgaagc ccccaataaa gcttggttga
     1321 aacaagatag gagcctcgtc tcaaaccaag cctgcgaaag gcttgaccca tgatgacttc
     1381 ggattgcccg cctgcatagg attctgcatt atcgaaatag tttacgccat ggtcgtaagc
     1441 atatttgatc atctgagatg cgctgtcgat gtcaatctgc gagccatagg tcacccatga
     1501 acccaaagaa agcactgata ccttgatacc tgcggaacct aaatgtcgat aaatcataga
     1561 atagctcctt tgcggaagaa tcttctgtgg caatgtccgg agcaagaatt cgcgtcaata
     1621 ctgagccatg ctttgagcca tgctttatgc aaaaatagta atccaataaa tcataagctc
     1681 actgccgaaa aatgcataac aatcggactt tgctctattt cccttgctcc gaattgagcc
     1741 cacattaaaa acatactaaa ctgagttcag aaagggaaga ttctgaatgt caaattctga
     1801 tagaatattc aagattgtcc atatttccga tctccatctt ggaaagactc ttagatctca
     1861 agatctagct caagatcaga gcagtatact caaacaaatt gttgaaaagg tcaaaatgct
     1921 caagccagat cttgtcatca ttgctggcga tgtgttcgat cggagcattc cttcagagag
     1981 tgctcagact atgttcggct ggtttatggc agagcttaga cgcgcgttac cgaaagacgg
     2041 acgcattcta gttattcctg gaaatcatga ttcacccaaa cgaatcgctt ttgccgcaga
     2101 attgttcgaa tctgtaggca tacacatagt cagcgaaatc ctatcaaacc cgacactcgt
     2161 gctaaaaaaa gatggaaaaa gagccgcagt ctgggcgctg ccctttgtca cccatggagc
     2221 ctaccacgag tttaagagga acttgaccaa aacgggctat gcagaaatgg cttctgcgga
     2281 ggcgaatagc tcaatggcag gtcgcatgga gagcattata gcaaacctcc gtccctccaa
     2341 atcagcctcc gaatccgaat acgacctgaa tatcctcgtg gcgcactgct atgttaagga
     2401 ggctgttcta tccgaatcgg atatcgcctt tattggcgga acagaagccg tgccggcctc
     2461 tcttttcgag agcttcgatt atgtagcact tgggcatctg cataaaatgc aagctctgtc
     2521 tccgaaagtc tggtattctg gagcacctat gtctatgtcc tttggagatg gagatatcga
     2581 gaaaggattt ctatatgtcg agctcgagga tggtaagccg gcaagagtac agcgaatgga
     2641 actcaagccc ttgcatgtct tcaagcgcgt gagaggcagt tttgacgaac ttgctgccga
     2701 atctgctctt atagatgatg attatgtcga agtgatcctt accgacgaag aaccagtata
     2761 cgatgctttt aatcgtcttg ctattcgttt tcctaatttg atgacggtac gccaggaagc
     2821 ctttgaaaaa agaataggga cagaggttga agaagtcgga attccggttc ctcatatagt
     2881 ttcggacgga gagacagaaa atgctgatgc tttggaagat ttttccggca aatcgcttca
     2941 tgctacggtt ctcaaggatt ttgttggctt tacgcagcgc attctcgatg agccgccttc
     3001 tgaggaattg ctgaatgctt tcgaggtact ccttagaaat gcggaagagg acctcctatg
     3061 aagccacttt atctttcttt ggagaacttt ggtccatatc gccagcgtca ggacatcgat
     3121 ttttgtaagt tagatgattt tttccttatc tatggcaaga ctggttctgg aaaaaccaca
     3181 atcttcgatg caattaccta tgcactctat ggcaaggcca ttgggggacg gagcaatctc
     3241 gagcgggaac tcggatcccg cttttctcca atcggaagca agccctgggt tgaattcgaa
     3301 ttcctggctt catctgccca atggaaggtg tatcgaagct tgccctacaa gaaacgtaat
     3361 aaaagagggg ttgaatccga ggctaccagc gaagttgagc tttatcggat gaatgaaatc
     3421 ggggtctatg aaataatagc cgatcgctct actacggcta atcaaattct gctcgatctt
     3481 ttgcgtctga gtgccgatga attttcgaaa atcgtactcc tgccccaggg agagtttcaa
     3541 aaatttctcg aaatgaagac cacagagcga gtggagattc tcgaaaagct cttcgatgta
     3601 ggtatctatg atgcggtaac cgaagcagcg cgaaaaaaag tcatggagct agaggcttat
     3661 cttaaagcaa agcaggagga aatcgagcgc atctcgaatg aactcggcat cgagcctgga
     3721 tctcgcataa aagaatcggg aaaagccatc tcagaattcg ataaaagaat cgaagaatcc
     3781 acgaaagacg cttcgcagtt ggaaactcaa atcgctcaaa atagagagcg tctttcattc
     3841 ctgcaatcaa tgcttgcggc ctggaaaaat cttcatgaaa aagaagagct aaagcctgtc
     3901 ttcgataaac gcgaaacaag acttgaagct gccaaatcgc ttggagaact ggcaagcctg
     3961 gctaagcaag tttcggctga ttacgaggaa ttaatcgagc ttctttcaga atccttacaa
     4021 acagggacag agctttccaa gctcgaagca gaagggatcg agatcaaaaa gatacagaag
     4081 ggactgccca ttgttcgaaa gaaatacgac aatctccagc gcagcgtggc attatatcag
     4141 aatgcagtga atgcatggaa tcaaaaagag caatgcgaaa gcgagcgcag acaagttatg
     4201 cagagactcg atgccacttt gcaggattgg gaatatcgac aaaacgagat cggtcagctc
     4261 aaatccagca taacagagat cgaaaacgcc atacaaagcg agcccttggc acaagagtca
     4321 tttcataaga ttgtcatcgc ggatgaagca ttcaaacgaa tagagacctg gatagaacaa
     4381 aaagaaaaaa tagaagctca acgcgtggaa accttacgta aaattcgaga agcggaagat
     4441 tctatccttc tgacaaacca ggagctcaca attgccgaac ggaatcgaga tcgcatcaat
     4501 gaggaaatcc gaaaggctca agccggtatg cttgcggcct cgcttattcc cggagaaccc
     4561 tgccccgtat gcggtgctcg ggatcatccc aatcttgcca aacttgaaca gaatgctccg
     4621 gatgacaaga ctatcaagac cgcccaaaaa gcggtggaag agaccaaatc atcgctctct
     4681 gccataatcc aagacaagca aaatctcgaa aaccgcatcg aagaattttc gcaggatctt
     4741 caagttatag tccaaaaaat ctctgaggaa tggaaaggca ttgcttgcga agtgattggt
     4801 gataaaagca caatgcagct tctggatatc tctccctcag agttcgtatc cataagctca
     4861 cgcttgcaat ctgagcgtcg cgagctagaa gaacgactta agagttatca acatggacgc
     4921 aaatcattac aagaacttag aaagactctt gatcaggcag ggcgagatct tgatcgaatt
     4981 caagaggaaa agacaagact cgagatgaag cagactgaat tagatgcaca tattgattct
     5041 cttggagagc agatcggaaa agaggatcct cggatttgcc taaaagaagc agaagaaaat
     5101 cttgtcactc tccataacga aattgagcag atcgaagagc aagcacaaaa atgggaaatt
     5161 gcctggagct ccataagtat caagatggac atgcagttgg agaaaattgg ccaaaaaggc
     5221 cccgctcttg ccacaatggt cgaggaattc ctcaatgagg tggacaaaat gaaagtgagg
     5281 agcctattgc ggcttctcga atctccggtg acttcttcgg aaaaagaagc accagaacat
     5341 gagcaacagg caatccggcc attgccagat ttcttgactt catcgggtga taatgcgggt
     5401 aatttatcgg gttgttcaga gtctatctat gagccttcca caagattgct gcaggagctc
     5461 tttgccttgc tagctaattc cttaccacat atcgatagat tcgtaccggt ctccgggcaa
     5521 attatgtacc tttcagagac cctggataaa atcttgtctg ctcatcgaat aaatgagccg
     5581 gcattaaaag cactgatgca agaaattctc tgttcggtgt ggacaagcaa tcgcatcaaa
     5641 gaagaagcca gggagatttc cgctttcaga caatcctata atgaagctag tatttcctat
     5701 caagtgctcg ccgcgcgcgc gcaagctctg tccccattca actcattgtc ccccaactca
     5761 ttcaattcaa ctacgctccc ctcatcaaac caatcgccag atcgattgcc ccaacataat
     5821 caagaaaatg gcgaccaagc aaaacatccg gaaatgttcg atggcattca tctcgagcaa
     5881 caagaaaaaa accttagaga ggaatgtctt tcccttctcg aaaggaagaa gagtattgaa
     5941 acggagatat cggatttgcg agacgagcgt gcgcgtttta tagcggagct cgatcagctt
     6001 cgtcgccttc tttcaaggcg agatgaattg actagcgaat atgaaagggc tatcaaggag
     6061 ttcggaaaac accaagagct ttcaaaactg cttggaggga gcttaaaccc tcaaagaaaa
     6121 atgccattca aaaactatgt gttgggcctt caattccgag aaatagccat gagggcttcc
     6181 agccgactct atcggatgag caatggccgt ttcctggtgg aagccgatat tttgggcggc
     6241 agcggcaacc aaaaaatcgg cctggagctc tatgtgctgg atgcctggaa tgggggtcga
     6301 cgcccagtgg ggacgctttc cggaggagaa aaattcatgc tctccataag ccttgccctg
     6361 gggttagccg attcgataca ggaacgagca agagcaaatc gtatcgaatc gctttttatc
     6421 gatgagggct ttggaagtct cgacgaggag tctctctcgc tggctatttc tgtcttagag
     6481 gaccttagag gcgacaagag cattgcgatc atatcccatg tggatgagct ctatagccgt
     6541 ataccatcga gaatcgtggt caaaaaatct gtggggggct cccatctaga gttagaacaa
     6601 gattaaaggc tcttctgttc attgctgagg cgccccatgg aaggcgcgct cgagcagttc
     6661 tctgagttct tcatcagtaa aagcacgtgg gctggccttg cgcctacgat aagaaagagc
     6721 atcgctaagt atatcttcga aataggacgg agatacgccg gcagcttcaa ggttctcagg
     6781 gatacctata tcatggccca gttggcagat tgcgcgcacc gattcttcgg ggctgatatt
     6841 gtccttagcc gttagcgctt gcccaatgtc cgataggcgt ccttttgcgt ctggaatcaa
     6901 ggattgcatc acaaaaggaa gaatgatcgc attggcaagc ccatgtggta tcccagctaa
     6961 tgcccccacg gcatgggcaa aaccatgaac cattcccaat cctgcctcgg agagagctac
     7021 tcccgcaagg gtcgccgaca gcgccatgtc gcgtctcgat tgcatggctt ttgctccctc
     7081 ggatatatgg catgccgacc tcaggctacg acctataaga cgaatggatt caaggcagta
     7141 catgtcgctg atgggcgtgt taaaacggct cacataggct tctatagcat gggtaagagc
     7201 atccatacct gtagctgcgg tgagagaagg cggcatgctt aaatgcaaaa caggatccac
     7261 aattgcaagc ttggggaaat aaaactgact cgaggtgccc tttttgcttc gacgaccgct
     7321 ctcgtccact acggtgaata ccgcggcatt tgtaacctct gtccccgtac cggcggttgt
     7381 cggaattgca atgagcggtg gaacgggctt tgtaaatgcc aaccccgttc ccacaaaatc
     7441 gcggatagaa cgaccttcgg caacgctggc tgcgattccc tttgcacaat ctatagggct
     7501 gcctccgcca aaaccgataa tggcgtcaca atgctgcgct ctataaacct ccgtcccttt
     7561 atctacaaga gaggtatcag gatccgcgct gacttctcca aaatgaacat accgtatgcc
     7621 acttgcatct aagccgatgc acagctctct gaaaccctgc aaagaagcgg ttttcgatct
     7681 gccgctcaca atcagcacat tggtcgctct catttctttg agtcgcgggg aagcttcggc
     7741 aaggcagcca tccccaaaca ataccttggt cggtgcatag aattcgtaca tagcatatct
     7801 cctatagaat tagctttccg aaattgccta tcctatcaag tataaacaag tttccaaagg
     7861 agtcttcagc aaagagcgct gcaataaagg aagctcaaat cagaactata tttctcaatc
     7921 atgtcataaa tccccaaaaa tcgttgagct ttatacctaa aatcttaaaa aattcttgca
     7981 ttgctcataa ataaagtata tagtatttga gaatgtaact caaagaggag gtaatatgaa
     8041 aaagatcctt ttcgcattga tcgcactggt tgcggtctta ggtcttagtg cccaggactt
     8101 tacaatcgta aatggtactg agcctacctc gctagatcca catgcggttg aaggtgtgcc
     8161 tgagcaccgc atctatgtgg cgcttttcga gggtctgacc atcaatgatc cgcgtaccaa
     8221 ccgagcgctt cctgcagttg cagagagctg gtcttttagc aaggattaca agacctgcac
     8281 cttcaagatt cgcaaaggtg ttacatggtc ggatggcgtc gaaataaccg ccgatacggt
     8341 tgttcagtcc tgggtgcgca agatggatcc acagaatgcc ttccagtatg ccgatctacc
     8401 cgcaatgtat atcgaaggtg gttcggatta tctcgaaggc aaggccggcc cagagagcct
     8461 taagatccgt gctgttgata aatacacatt cgaggtcaaa ctggttggcc ctattccatt
     8521 ctttgcagat atggtaagcc actatgcctt cgccatcgtt cctatgcacg ctatcgaaaa
     8581 atatggcaag gattgggtaa aaccaggcaa gatggtatcg aatgggcctt atgtcctatc
     8641 cgaatggaag ccccaggaaa aagttgttgt tgtaaagaac aacaagtact gggatgcaaa
     8701 gaacgtctct ctcaagaagg ttaccttcat tgctaacgac gacatcaatg ttggctataa
     8761 cctctacaag agtggagccg ctgactggac agaagccgtg cctctagaac tcatggatga
     8821 ggtcaagcta cgcaaagact tccatgttgc tcctcaatat ggaacctatt attatatcta
     8881 caacacaacc cgaaaaccgc tcgatgatcc tcgcgttcgc aaggcgctcg ctatggctat
     8941 caacaaagac gaccttgtca acaaagtcac ccgtggtggc cagatacctg caaatagctt
     9001 agtgcctcct agcaccggct atactccagc aaagggttac aacttcgatc ctgcggcagc
     9061 acgcaagctt ctcgcggaag cgggctatcc ggatggcaaa aatttcccgg catttacgat
     9121 actctataac acttcttcgt ctcacaagag cattgcggaa ttcatccaag cccagtggaa
     9181 agttaacctt ggcatagatg tcagccttat gaaccaagag tggggtacct atctcgacac
     9241 ccgatcccag tctcataact tcgatatcgc tcgagctgga tggattggcg attatctcga
     9301 tccatcgact ttcttggaaa tgttcataac tggcggaacc cagaatgatg gtctctatac
     9361 caacccgaag tatgatgaac tgctcgacaa agcgcgtgtt gcgagcggca ctgagcgctt
     9421 ccagtatctt atggatgcag aaaagatcct catcgatgaa gatatggcag ttcttccgct
     9481 atatttctat gtgacccaga acctcatcga tctggacaaa tgggatggct ggtatgcgaa
     9541 cccgctcaat atccatccat ggaaatttat ccgacccaaa aaataaaaat tacctaaagt
     9601 aagtaagcag ggctgtccga tacgggcagc cctttttttc ttgggcaaat tcttgcagta
     9661 tcaattctaa ggaaatattg gctagataaa atttcatgat tggagtataa tgttttcgct
     9721 tgagaaaagc cttctcatgt ttcgagattc caattgcgca aatatattct tttagaataa
     9781 tcaatttgcg actcggaaag gagtaattcc gaatgccacg atatttcatt cggagaatat
     9841 tgagcataat tcctacgctc tttatcgtga tcacactgag tttttttcta atcaggcttg
     9901 cacctggggg cccttttgcc cgcgaacgtg aggtgccaga ggcgattctt caaaatctca
     9961 tgaagcgcta tcacatggat gaaccgctcg ttaagcaata tttgcgctat atgggcgacg
    10021 taattcgttg ggatttcggc ccttcctacc gatataggga tctaagcgta aatgagatta
    10081 tagacgaagg tcttcccata tcgatgtcct tgggtgttat ctccttggta ctggcgacgg
    10141 tcggaggaat tgccatcgga attatttcag cgttgaagca gaacagatgg caggattatg
    10201 ttgtcacttc tatcgctgtc ataggcattt cggtaccgtt atttgtgatg gggcctgtct
    10261 tacagctcgt tttcggaatg aagctcaaga ttttgcctat aggtcaatgg atctcgactc
    10321 atggtataaa agcggtaatc cttccggcca taactctatc gtttccatat tttgcctata
    10381 ttgcgaggct atcacgagca agtattctcg aagttctgcg atctgattat atccgcacag
    10441 cacgagcaaa ggggcttaaa gagtcagtgg tagtttggaa gcacgtgctc aagggagcta
    10501 tgctccccgt agtcacatat ctcggcccag ccttctcagg tattgtagta ggctcaatag
    10561 tcgtagaatc ggtcttcctt gtacccggaa ttgggcgccc ctttgttcaa tctgccctaa
    10621 acagggatta tacacttata atggctgaag tggttgtata ttccattatt ctcatcattg
    10681 caaatcttgt ggtcgacatt ctctatggct tcctcgatcc aaggatttct tacaagtaag
    10741 gggtgggaga tggcaaaaaa acaacttgaa actgcagtca acgagttcaa ccaggtaatg
    10801 aagcccgtct ccttatgggc tgacgcatgg aagcgccttc gaaaaaatag gatggctttg
    10861 ataagtctcg taatagtatg tatatatttg gttatcagta tttttgcgcc tcttcttacg
    10921 agtgctggaa ttctcatcga ttaccgcaaa caagtcatta ccaatgcgag cctgcctcct
    10981 tctttcaaac cagccggaga gctggttatc aataagatcg agaagcgcat cgcggatctc
    11041 gaacaaagag tcgaggaaca aaaacagcag cagtctgcct tcgattcttt cttcgatttt
    11101 ggatccgtgt atgctcagga tgaaaacggc gaagccggcg atgatttcgc accaagccag
    11161 gaggaattct cttttactta tgaaggtgaa ggattctttg gcaataccac cgatccggta
    11221 gtccgcgaac tcatcagcca aaaagaaacc ttagaacacg tcaaagccga gctagataca
    11281 aaccccgact acaagatcgt atacattctt ggaaccgata atctaggccg agacatgttt
    11341 gcacgcataa tctatggtgg tcgcatttcg attgccatag gagtcgtagg cactctgaca
    11401 gctgctttgg tgggtattct ggtcggagca atttccggct atgtcggtgg ctggctcgat
    11461 aattttctta tgcgttttgt agatatcatg tatgggctgc cttatatgct catcgtaata
    11521 atcatcatgg ctatgatcgg cgagaaagct cgtgggagct ttgtggtgct ctttgtcgca
    11581 atagcattag tctcttggct tactattgca cgggtggtac gagggcagat tataagcctg
    11641 aaaaattcgg aatttgtcga agcagcacgg tctatgggtg caagtactca acgcattatc
    11701 ttccggcatc ttctgccaaa cactctagga gtcatcattg tattctccac actgatgatg
    11761 ccaagcttta tcatgaatga gtcctttctg agctttttgg gacttggcgt gtcggcacca
    11821 gatgcatctt ggggcactct cgtgtcggaa ggagttcgtg ctatggaatc ctatgcctgg
    11881 caattgcttg gtccgggtat tacaatgacg atctttcttt tctgcatgaa cttcctgggc
    11941 gatggccttc gagatgctct cgatccgcag agtaagaacc gcacttgata aggagcctaa
    12001 atgtctgatg aagtcatcct tcaagtaaga gacttgaaaa catactttac cgtagatgag
    12061 ggaatcgtga aggcagtcga tggcgtaagc ttcgaacttc accgcagtga aaccctcgga
    12121 attgttggtg aatccggatc agggaaaagt gttaccaatc tctcggtcat caatctgatt
    12181 ccgacacctc caggcaagat tgtcggggga gaggtgcttt ttcatggcac tgatctgctc
    12241 aaattgccgc ccggcgaaat ccgccatata cgaggcaaca agatttcgat gatcttccag
    12301 gatcccatga cctcgctcaa ccctttcttg cgaatttcta cacagatgat cgagacaata
    12361 cagctgcacc aggggctaga taagaaggcg gcaaaggaaa aagccatcga aatgctcaga
    12421 cttgctggca tccctgctcc ggaaaagcgt atcgatcaat atccgcatca attttcgggg
    12481 ggaatgcgac aacgagtcat gatagccatg gccttatcat gtaatcccga gatactgatt
    12541 gccgatgagc cgacaagcgc actcgatgtt acaattcagg ctcagatcct cgatctcatg
    12601 aaggaattaa cccgaaagct tggcaccgca gttattctaa ttacgcattc gctgggcgtt
    12661 gttgcgggta tgtgcgacac gatctgtgtc atgtatgcgg gacgaatcgt cgaacgcgga
    12721 cgcaccgaag atatctttga aaatccgaag catccttata ctatcgggct aatccgctcc
    12781 gtaccacgcc tcgatcaaga gaccaagatg cgtctatatt caatacccgg tcagccgccc
    12841 aatgtaatag atttgccaga ttgctgccca ttctacccac gctgcgaaca atcaatggat
    12901 atctgcagag ggaaataccc atctattgta tccttcgaga acaatcagag cgtagcttgc
    12961 tggctctaca acaaggagac tcagaatgcc tgaaaccgac gaagtacttc tagatgtgcg
    13021 cggcctcaag atgcatttcc ctgtcaaaac gagcatgttc tctaagacga agaattatat
    13081 atatgctgta gacggaattg attttaaggt aagaaaaggt gagactctgg gtcttgtagg
    13141 agaatcgggc tgcggaaaat cgaccacggc tcgcgccata gatcagctgt acaagccaac
    13201 tgctggtgag gtcattctaa aaggcaaaga tctcactaag ctaccaccca gcgagatgat
    13261 ggaagcgcga aagaacatac agatggtttt tcaagaccca tacgcttcgc tcaatccacg
    13321 catgaccgca ggcgatataa tcgcggagcc cattcgcatc ttccaaaagc aagggcttat
    13381 agagatgagt gcgcaagatc gcaataatcg ggtagaagag ctcatggaaa aagtggggct
    13441 gtcgcgatat ttcaaaaacc gttatccgca tgaattttca ggtggccaac gccagcgtat
    13501 cggaattgcg cgcgcattgg cgctcaatcc ggagctcatt ctatgcgatg aacctgtatc
    13561 cgcgctcgat gtctcgattc agtctcaaat tctcaatctt ttcaaagacc tgcaagggga
    13621 gtttggccta acttatcttt ttattgcgca cgatctttct gtcataaaat atatctccaa
    13681 cagagtggct gtaatgtacc ttggactcat tgtggaaatc gcggacgcag cggatctata
    13741 taagaaaccc ctacatcctt atactgaggc acttttgtca gcggcaccga ttcctgatcc
    13801 aaagatcgag gccaagcgca agcgcataat cctcactgga gatgtgccct ctccagacaa
    13861 acagcgacca ggctgcaatt tctacgatcg atgtcctaag cgcatggata tatgcaaaga
    13921 aagacgacca tcgctaaaag attcggaagt cgggcacgct gtgtcgtgtt tcatatttca
    13981 caagccggag tgatataggc aagagagatt ctagatagag cctgattagt ttcttgctgg
    14041 cttccctcaa cagggctgag tcaggtcaag atgaggacaa cacatacaaa agacgaggcc
    14101 gccccgccca ttcgggcggg gcggcggcct tttcccataa tccttcaaaa ggtccctaaa
    14161 aaccactgca tcataatatg ataatgattt ccgataacta attcctatat cccgcttgac
    14221 aaaaaagaat aaataatgca ttttgcttcg aaagactaac gccatgaaag ctaatagcaa
    14281 ttatatatgt ttcatgcgaa gcggctcaac gaagccgctc tccgcgaccc gcgccttctt
    14341 tctagctatc ctgtaaactc aagcctatac caaaaatcca atctctgtct ctatccaggc
    14401 aaatttccga gccaatctta tagatcttgt cagccacaac tcaatgtctc gaatctatct
    14461 gtttctctta gagatgccgt atggcgtctt ataatgattt tttatcctgg aggtttctta
    14521 tgaaccgcaa aattgtcacc tttatctttc tagcctcttt agtctcaacc atgcttgtct
    14581 ttgctggccc cttttcctcg ggtacgctaa gggttggcgc tacttccgtg ccccacgctg
    14641 aaatacttaa tttcattata cccgacttgg cagcgcaggg aattaagcta gaggttatcg
    14701 agttcagcga ttatgttacg cccaacatag cactagccga aaaacagctg gatgctaact
    14761 tttttcagca cctgccctac ctggaaacct tcgctgccga gcgaaaactc tcacttgaaa
    14821 gcgtaatagc cgtccatgtg gaacctcttg gtctctattc gaagaagtac aagaaagttt
    14881 cagattttcc atccggttcg ataattgcca taccaaacga tccgacaaat gagggaagag
    14941 ctctgctatt ccttcaatct aagggaatca taaagctcaa gccagacgct ggacttcagg
    15001 caacgattcg ggatattgtg gaaaatccaa agaaatttgt atttaaggaa atagaagcgc
    15061 cccagttgcc gcgtacactc gacgatgtgg caggctcgat catcaatgga aactacgctc
    15121 tccagtcggg ctttaaccca ataaaagact ctcttcttct agaaggtgga gaatcaccgt
    15181 atgccaatat ccttgttgtc agaaaaggcg attctaatga cccccggatt caagcactcg
    15241 cgaaagcgct cacatctcaa agagttaagg attttatctt aaatacttac aaaggcggtg
    15301 taattccagc cttttaatcg gcctctctgc tcaggcgtag tgatccgact tatcgatccc
    15361 gccttttgct caaaaggagc cttgcgctga tccattagcc ttttattgtg caatccattt
    15421 attggacagc atttccaaaa atgccgtcac tgctagaaga tcggcgcatc ctccagggct
    15481 aatgccacgc tgactgaagt cagcatcgag cttctggaca aaatctcgat tctccatcag
    15541 cctcattcct ccgcgcgaaa gagcagcagc agcctgagct ctgacatagg caagaccatc
    15601 cgaccccgct ctttttaata ccgtagtatc gtctaccttt aggtagagga ttagaagcgc
    15661 atcgaccatc gcatcgttcc atgacatgcc cgccaagaga tcgaaatgta gccttggcaa
    15721 agcatgattt attacagaag gaaaaccatc ttccgcttcg ccacggactc ctcttattcc
    15781 atatttgatg aacgattgct ctccggcgct gagatcaata gaaggaacca cagcattttt
    15841 cgctcttatc gtgtcggaag cgagaagcat ctctgcttcc acaagcggaa caagttcgcg
    15901 aatggtgatt ccgctaacga tatcggcaac ctcttgtata catcccgata tgacagcgct
    15961 tctatcactt atctcggctc gcctatccga aaccaaaagc ttcccggccg ctgcgcatgc
    16021 taagcccatg gaaaaaatga gccctttatg ggtattgatt ccctgtgtag ccgcatacat
    16081 ggctttttca gcttcgattc cgagctttcg cagttctgcc agaagtcctg ggctttcgcc
    16141 tgcaaattct gcaccaatgc gggcaaaatc tacaaaaaat ggtgctaggg ctgccgcgct
    16201 cgcaaggaag gtaaaataat ccatgtctga gtgtgcgcct ctagagaaag gatcgaccag
    16261 cccgggtttt ggtgctgccg agacttcata aagcgttgcg gtcaacgcga gattccccac
    16321 ttgcgcaatc aaatcatccc aatacatcga tttgtctcaa taatcaatct caatcgcgtg
    16381 ttttatggct atcataagga ttttgagctt cttaaggcca tcgaatctga tatgatcgcc
    16441 tcgatatgcg ctgcaatatc caaaagagaa tgacactgac cggcggaaca taccatagcc
    16501 tcattatcac agacaaggca ttttctgggc tcaaaaccca tatcccttcg cgaaagaagt
    16561 ctagcctccg catccgtcac atcgatatcc cctagcgcac ccagcacatg tccttcctct
    16621 atggatattg ccatccgctt caagatctct tcatcgcaac aagaggcaag ccaagcactt
    16681 ggcccatcat cgtactgttt ctgacctgaa gctatgattg tgaaagcctt ctccattagg
    16741 gcttccgcga gagcatcccg caattgcacc gcaatctctt tataaggacc ataaattctt
    16801 agagaagccg gcattcttag agtgcagaaa gcaaaggcta atctatgccc cgagaattct
    16861 tgagccaatg attttgcaag cagctcttct tccttccaac gaagctcgcg agccgcaagc
    16921 acggtttgca aacttaacac acaatacttc tttcgattgc agtctctaag cgagcacgaa
    16981 gagtacattc aagagctcct ctatcctgaa catggatacg cgctcctgta attccagcac
    17041 tctctgccat ttcctcggtg atttgtcgga tccttttccc atactgttta attactatgc
    17101 tttcaatgtc gtactctatg tggtcttctg acacaggcgt tattgttacg agcacgtcgc
    17161 tggattccag cgagccagca gtaactgatt tgagcactct cattgtcttc tccaaatttt
    17221 tacctccgaa attagatcaa aacttatcag tgcctcgaat cgctttggcg aattctctct
    17281 attaccggcg ctgcctcctc actttttagc caatcccatg tggtccctgg aacaaatttt
    17341 tgtacctcgg ataaattacc ttcgcgaatc atatctcgaa ctttagatgc acttacggga
    17401 gtcccccaca gctcgaaacg gggaatttcg tgtaacgcta ccggtggagt ctttgcatct
    17461 aatccaaaag cctccgtctc tttggcttga agaatgatct tcattatatc gttataaatc
    17521 gaggtggtgg gacagtatgg ctccgttcca acaaatcgat cggtgacttt gagtgcaggc
    17581 gcgatgtgcg tcctaaaaat gctgacatcg agcgaagcgt gcattcttac cgctttccac
    17641 gaggctttat tcacatccgg acgaataaaa taagttggaa atgtagcaga agagataata
    17701 tagggtccgc ccttcattac cgtcacattt cgtaaatctg cgactccctc ttgtacaagt
    17761 ttgtatcgaa ctttgaaagg aaataaagat ctatcctcct ctacgaccag cacatagagc
    17821 cagggtacat gcgaggatgc atactctata agatatctat ggccaagcgt aaatggattg
    17881 caatttatta ctactgctcc ggcacaatcg ggcttatctt tggccaaggc ccgtatggag
    17941 gccaaatagg attcgatacc ggccgttccc cactctagca acgcggctcc tcttggaacc
    18001 gcagctacct tcctaaatcc caggctctcg aacctggata tatcttcgct tatggtgtat
    18061 agaaaaactt gagttatccc tcggtcgacg gctcttctaa ttagggaaga gactatagcc
    18121 gccgatattc cttcgccttc ataatctata tcgactgcta taccctgcag aacattgcgg
    18181 acaagagcgc ctgtagcaac gagctgctcg cccacgaaga gacccaagac cagctcttcc
    18241 ccttccggaa ccataagtcc gcgggaggtc aaaagcgcct ctcgttgtgc gagctcgtcc
    18301 tttgaattaa gaattctctc ttcaagccca aaattcaact cgctcatatg tttaccgatt
    18361 atattttcta atctaccggt tcgatagcct tcgacgcgcg gcatttagcg ctccgccggc
    18421 tagtataatt tcaatctgtc gctgtgaaag cccatgatga gccataaaag tagtacctct
    18481 actttcgttc tttacctcga agcgatcaag tcgcaaccct tcgtgtatat ttttgatata
    18541 gagcatatcg ccctggctga tattttggat ttcttcggaa ctatcgaata ttaaaggaag
    18601 aatgccggca tttaccaggt tttgcctgtg gatccgcgcg tatgagaccg caatcactgc
    18661 cctaatcccc aaaaaacgag gcgaaagcgc cgcatgttca cggcttgagc cctgcccata
    18721 attttgccca gcaacaagaa tgccgcttcc tgcagccctt gctcttgccg gaaagtctgc
    18781 atctagtacc gaaaaacagt actcagaaat agcgggcaga ttcgatcgca gagacatgat
    18841 tctgcttcca gcgggcaaaa tatgatcggt tgttatatta ttgcccatat gcatcagtac
    18901 ttcggctctg agctcatctc cgatcgcctc gaattgcggc aagggcgcta tattgggtcc
    18961 acgtcgaatt cctgcttttt ctccttcggt cgatggaaaa ataaacatag aatcatcatt
    19021 gataaagctc tctgggatcg atatagtcac acgcgatttg actgtgcgag gatctgtaat
    19081 gacgccggct attgcggaag cagcggctgt ctctgcgctt gcaaggtata gcctggctga
    19141 ttttgtcccg gatcgccctt cgaaatttcg gttgctcgtg cgaagagata cagcgttcgt
    19201 tgctggcgct tgtcctattc caatgcaagg gccgcatgcg cattcaagaa tccttgctcc
    19261 ggcttcgaca aagctcgtca atattccatc cctggctgtc attagcaaaa cctggcgaga
    19321 gccaggagcc acgacaagac tcgtttgggg cgcaacatga tggcctctca atatcgatgc
    19381 gctgaccgca agatcataat aggatgaatt tgtgcagctg ccgataaaac actgatccac
    19441 gggcaagcca gcaatttcgt ggactggcac cacattgtcc ggcatatggg gcagcgctat
    19501 catcggttcg agagtattga gatcgacaat catctcctca tcataactgg catctgaatc
    19561 ggcaaatagc ggaatgaaat cttctgctct tccctgtgcc tccaaaaagc gtttagtaac
    19621 ctcatcgctc gggaataccg aagtggtcgc gcccagctcc gcgcccatat tggcaatagt
    19681 gcctcgctgt ggaatgctca gtgtcgatac tccctctccg gcatattcca gtatcttgcc
    19741 aacaccgccc tttaccgtaa gccttctcaa tacttcgagc acgatatcct tagcgctcac
    19801 cattggagac aatctgcctc tcaattccac tttgagaacc ctgggtctgg gaatggagaa
    19861 aggcaagccg gccattgcaa gcgctacatc taacccacca gctcctatgg caagcattcc
    19921 aagcgctcct gcggtaggag tatggctatc cgatcctaat agcgatttgc ctggaactcc
    19981 aaaacgttcc aggtgcacct gatggcatat tccgttcccc ggcctagaaa aaacgattcc
    20041 atatctcgat gctatatcct gcaggaaatt atgatcatct gcattttcga aac
//
LOCUS       DAOOWY010000027        19792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2731_length_19792_cov_10.310280, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000027 DAOOWY010000000
VERSION     DAOOWY010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 19792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19792
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2731_length_19792_cov_10.310280"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..392
                     /locus_tag="PK105_03625"
     CDS             132..392
                     /locus_tag="PK105_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60829.1"
                     /translation="MNSDYPVLVEEKLPRMRRYSTLGAIRRELMRLYEEAKKQKQLDP
                     IEIQRYRMLCFLLSSAGELMKAEKLDEIERRISVLERQGGRK"
     gene            389..652
                     /locus_tag="PK105_03630"
     CDS             389..652
                     /locus_tag="PK105_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60830.1"
                     /translation="MNLEKRLAKLEAQNDGSALDIFVQYINEQDEKKANALYKKYLRA
                     DPNQEIEEFLKIIDRRVKIAKKRSEENERLIANSEWRIVEDHI"
     gene            complement(775..4317)
                     /locus_tag="PK105_03635"
     CDS             complement(775..4317)
                     /locus_tag="PK105_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60831.1"
                     /translation="MAKSKQLSTPISTGGAGAFFEAQVQSMFVTLMLSGGYAPCLPCW
                     PIIEIKLQGKVDGFDTDDLIVYVKNANTGERRKLLAQIKLTITITNEDRRFDEVIQAA
                     WNDYNNSKNFKKGKDAIALITGPLSATDTHNVQWILEQARHTKNTDEFFTHVHQAKFS
                     PSKSTEKLEVIEKHLKAANGGNEVERDKLYSFLRHFHLLVVDLDKESGVVLSLLHSHI
                     SLFKPLNVEWVWPRIVAFVQEWNRNAGTIAPDKLPEDLQNAFNQKFLVAIPDELRVAQ
                     ENSEIDWAQHPDATFIALLVLIGTWNEKNQSDIDVISQMLGINYDEWLKKAQELLHYH
                     NTPLSVKNGVWKINNRTELWNLLGSRVLDKNLDTFISLAVTILKERDPAFELPVEERF
                     AASIHNKVFLYSHEMRKGISEGLAVLGSSSNACIHCSVGKAKENCKLALIQLFSGADW
                     ILWGSLNDLLPALAEADPGEFLNAVEKALRQIPCPFYRLFSEESSGVFGRNYLTGLLW
                     ALEALAWEEQYLVRICVILGELASHDPGGQWSNRPSNSLVTILLPWFPQTLASVDKRK
                     VAVQILLKEWPDIGWNLIIQLLPNQHTISSGSYKPVWRKTIKDQGSKEVTKEEYLKQI
                     SIYAELAVHAAECDANRLSDLIQHLDSLPPSVFNQIINRLDSPPILQLAEDQRFLIWD
                     SIRKFTVKHRRFSDAQWALPGELIIRLEKVSEHLIPKCPSIRYKFLFTTRDLDLFEKN
                     SDWKEQQKQLDVKREAAIKEIFDQNGVEGIIRFAESVDSPRKVGVACAAIDDFKIDAA
                     FLPHFLDPDDAKRNDLVKGFILQRQYLYGLDWCDNLDKSNWTPAQIGKFLACLPFIKT
                     IWDRASEWLKEQEQEYWTRVEAIPYQCDGDLNIAIEKLIKYNRPYAAINCLSRMLYEN
                     QLLNTDQCVRALLAISSSDHPETSMDIHNIVEIIKHLQTENSAKEEDLIKVEWAFLPL
                     LESYSEETAPKFLESRMANVPEFFCEIIRLIYRPKNEAQPPKELTEKEKSVALNAWRL
                     LQGWKILPGTQKDGTFDPENFKQWLQRVKELTEASGHLEVAMNHIGEVLINSPADPNG
                     LWIHQVVAQALNDRNFNHLRRGFMLGFINSRGAHSIVPTGEEERNLANQCRYKADAAE
                     NMGFHRLAATMRELAEHYDHEAERIIDEFNNDDE"
     gene            complement(4362..6407)
                     /gene="brxL"
                     /locus_tag="PK105_03640"
     CDS             complement(4362..6407)
                     /gene="brxL"
                     /locus_tag="PK105_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HOU60832.1"
                     /translation="MSEMDNLDKKAATKLEGYLVRKDLVRTFSRQFPVPTYVVEFMLG
                     RYCASTNQEEIEEGLDIVQRQLMSRTVKAGEEELFKARAKENGEIKIIDIISARLDAR
                     TDSYIATLPSLRLNDVRISSELVKKHERMLTGGFYAEITLSYDATIAQENKGRPFGIE
                     SLREIQLSKHDVLDILADARKSFTSLEWKNLLLRSIGIEPLGLSDRQRDALLLRMVPF
                     VERNYNLVELGPRGTGKSHLFQQISPYAHLISGGKATVAQMFVNNSTGQRGLVCLYDV
                     VCFDEVSGISFDQKDGVNILKGYMESGEFSRGKENIRADGSIVLIGNFEVDVDHQQRI
                     GHLFGPLPPEMRDDTAFMDRIHAFLPGWDVPKIGNELLTNHFGLVSDFLSECWSQLRN
                     QNRVSILQNRVYYGGALSGRDTNAVNKTASGLLKLLYPGEGEILDEDVEWAVRIAMEA
                     RRRVKEQQKRIGAAEFRNTHFSYFMGTEGVEKFVSTPELQSQNTIGGDPLEPGQVWTI
                     SPGGSEEHPGLYRIEINEGPGSGVKILNKPVPPAFKESMGYAEQNLYARAVQLVGDKD
                     PRQHEFTVQLRAFDVSKSGAKLGMASLIALCTALLKKSVRGGLIIVGEINLGGSIEPI
                     HNSVTIAEIAVEKGASALLLPVACRRQLLDLSDDMATKIDIQFYSDARDALMKAMIE"
     gene            complement(6404..8809)
                     /gene="pglZ"
                     /locus_tag="PK105_03645"
     CDS             complement(6404..8809)
                     /gene="pglZ"
                     /locus_tag="PK105_03645"
                     /inference="COORDINATES: protein motif:HMM:NF033450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type B"
                     /protein_id="HOU60833.1"
                     /translation="MRVLDHLLKDIRSAAVYNPEIQVPPACILWPDHDRQWETAISRL
                     QSEMPELLVLGDYEPEKEIGPAIWLRCIIAGINDPHITQANADLSSIVAKIRSSMRST
                     NTIIPVLYLPGVSRRDLRAVETCPDYLKALAELQFRGVIWSQINSKDWTILAYLKSNQ
                     GGLGLDVAQDNDTKKAMQLSLYRLLDEDVSLLQAKHLDKDYFNTLLSGNDLIRDLLQW
                     LDQGEVFQRNRDKNEWEAFVEVCESQLAYHPQKEGILTGITKLANHQGPWHAVWERYC
                     EASKRYANIPNKIRKCTPPQWDLYTDSDSACGWPQWNDDQEKSLQYDLLALAQTTAHE
                     ARKKLLKLEEKHGGRRSLVWAELGEAPLAFALQYLAILAEITQNSLAAGSLDDLISGY
                     RSHGWQADDAVLKALAQVESSKDIEAVIAAIRAIYLPWIEESARYLQNLVDSTSYPGG
                     TCLTPKKVSFSPGDCILFVDGLRFDMGKRLISTLEARNYKVTEELAWSALPSVTETGK
                     AAVSPVRDRIFGEDSSTDFEPSVIETGQSLKGGYNLKKLLRDSGWAILENSNAGDSQG
                     MAWCEFGDIDHEGHARGWKLTKYIDSLILEITDRITELLAAGWKRVRVVTDHGWLLLP
                     GGLPKINLPSALVDSKWGRCAALKPGATSDVRLFPWYWNPNQYFALADGVSCFKRGEE
                     YTHGGLSLQECLTLHLLVTQEASEQTATSVKIAGVVWKGLRCTVTVEGYFSGLAIDIR
                     SHAGDASSSVVVDKKTLKDNGTTSVIVEDEDMEGQAAIVVLIDENGSLIAQTPTVIAG
                     GNP"
     gene            complement(8821..10602)
                     /locus_tag="PK105_03650"
     CDS             complement(8821..10602)
                     /locus_tag="PK105_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HOU60834.1"
                     /translation="MIKSVNNYPVSQLFDIEANVVYVIPRYQREYTWGKNQWENLFDD
                     LRENESGYFLGSIICINQSIDALSAQNLELVDGQQRLTTLSLLFAAVYKTLKNHQNDL
                     DDDQRIELINLKRKLVLKKGDDQIRLIPQIQNNNNADYRAVLAEIGVISEYDAPAYAG
                     NRKIYRAYRYFQDRIDEMANGTSNQLETIIEFLDKVNQTYLVKIEVASHADAYTLFES
                     LNNRGMPLTAIDLIKNKLLARLESLEPGKIDYYFTHWNRLLQYLGDDYVIQERFFRQY
                     YNAFKNELKAVHQVPVATRSNLIQIYEKLIDYDAKDFLQKISISGRLYSLMLSRNKDD
                     TYRELEKSLKDLERIQGAPSYLLLLYLLAKKEELELTNAHLQSIIELLVRFFVRRNLT
                     DTPPTRDLTRLFMTLIDKLGELRADAILQSMRQQLVAVSATDQVFESKLKGPIYEENS
                     GVTRFILCALAEQSMTKESWVDLWRIENNQFVWTIEHIFPQGENIPQSWINMIADGNK
                     EKAKEIQQTHVHKLGNLTISGFNSALGNKSFEDKRNRTDRHGHAVGYKNGLKLNEDLA
                     SAERWSVAQIDSRTEKLVQQVVQLFKL"
     gene            complement(10599..14036)
                     /locus_tag="PK105_03655"
     CDS             complement(10599..14036)
                     /locus_tag="PK105_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60835.1"
                     /translation="MQPLNKTLRNLLERTIRNARDIAEDAARAALEQLAVGEAEPFAH
                     LSEQERALRRRLRAHGRQLGDSLNGSEAQTMDRLIEEVAYEHWHRMLFARFLAENNLL
                     MYPDPNEPVPVTLEECEDLAVDEGATNGWELAARFASRMLPQIFRIDSPVFQLSLPPE
                     HQQKLERLLADLPPEVFTASDSLGWVYQFWQAKKKKEIDDSEVKIGSRELPAVTQLFT
                     EPYMVSFLLDNSLGAWWATRRLTEHDLKNASSEEELRRRAAIPGMPLDYLRFVRQENG
                     AWIPAAGTFESWPDNLSQLKVLDPCCGSGHFLVAAFLMLVPMRIELEGLSAREAVNRV
                     LSENIHGLELDQRCVELATFALALTAWKYPNAGGYRVLPELNVACSGLSISAKKEDWL
                     ALAGNNTKLLLSLEELYKQFKDAPVLGSLINPEASLGKGSLFELRWEEVEPLLTKALV
                     GEKDDEKAEMGIVAKGLNKAARILGQKYSVTMTNVPYLAINRFSETLRIFVEKNYAKA
                     KNDLAFVFVKRLCSLTETGGLACFVTTSGWTFQNRERHFRNEMLTSTSWKIMARLGAG
                     AFNTISGEVVQSMLGITSNQKLKTNFYTIDVSDESGVDRKADGLRRKFTNSLNQIELQ
                     NSTHTVINFSPNSREKPLGEYAKALRGLSTGEVNWFVKYFWEIELPNNAWEYFQSDTS
                     HTVPFGGREQVIFWESECGTIARIAEELKSLNHAIQSWRRGKPNWGKTGVAVNLMGRL
                     YATIFTGNKYSANIGVLIPKKPEDLCALWAYCSSDDFVSSIRSFNKKISVEPRYFEKA
                     LVDFEVWRRKTSLETLPTEPYTDDPTQWIFHGHPCGSVIWDEQKKGTSNGPLRTDATV
                     LQVAVARLLGYRWPAELDPEMELADEQHKWVERCKELLPFADQDGIVCIPPVRGEAPA
                     ADRLLNLLAASYGDAWSNDILATLLKNADHVGKTLESWLRDKFFTQHCKLFHHRPFIW
                     HIWDGLPDGFSVLVNYHKLDRKNLETLIYTYVGDWINRQKQDIASGVDGAQERLAAAE
                     ALKKKLELILAGEAPYDIFVRWKPIEKQPIGWDPDLNDGVRLNIRPFMTVPDVQKKGA
                     GILRDKPNIDWKKDRGNDVKSAPWYHLGPQYGGHEGDRINDHHLTLAEKRTAREAAK"
     gene            complement(14060..17497)
                     /gene="brxC"
                     /locus_tag="PK105_03660"
     CDS             complement(14060..17497)
                     /gene="brxC"
                     /locus_tag="PK105_03660"
                     /inference="COORDINATES: protein motif:HMM:NF033441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HOU60836.1"
                     /translation="MNNRDIYQKDPSSRKLVNEGVASVNDENTSQALAVLRYELETFV
                     CEGQYEKGLYHILDTYLRNINQAQQPAVWVSGFYGSGKSHLVKMLRALWVDTVFDDGA
                     TARGIANLPQNIRDLLKELSIQGKRHGGLHAASGTLGASANGSVRLALLRIIFKSAGL
                     PEQYPVARFVMWLKHEGIYDQVRTLVEQKSFDWNEELDNFYVAEGLHDALVQAKPNLF
                     SSSSSCVETLNNLYPYVQDISSDDMIKAIRQALTKDGKFPLTLVVLDEVQQFIGEDSQ
                     RSVDVQEVVEACCKNFGGKLLFIGTGQTAVTGTSNLKKLEGRFTIRLELSDADVDAVI
                     RQVILAKKPEAKAPIERIMQLNLGEISRHLAGTTIGHRQEDISNYLQDYPLLPVRRRF
                     WENTLRVLDQTGTDSQLRNQLSMVHKVIQTNLDKPLGNVVPADYLYFDSADKLLQSRI
                     LPRKVHEKTMIWSRGSEDERLMARACGLVFLINKLGSQNTEIGIRATIDTLADLLVEN
                     LSDGSSSLRSKLPSLLDTCELLMKVGDEYRIQTEESAAWNDEFLSQRSALSNETHRIE
                     AERDERIRKKFNEIVGKLSLTQGNSKVIREISPLFDAQLPHDADQRIYLWVRDGWSID
                     ENSVRADARQAGIQSPTVFVFIPKRSADDLRHHLIDYKAASATLDKRGVPNTPEGIEA
                     RAAMETTKQSAEKKIGELLEESFSAARVFQAGGNEIAGNDLQEMILEASSNALQRLYP
                     QFQIADHTGWAKVYEKAKTGAPDALNAVDYEDEPAKHPVCKAILAFIAGGKKGADIRT
                     KFESPPYGWPRDAIDGAMQVLLVAGLIRAQDEHVIILDPKELERKAIGKVVFKVESAT
                     ITTAQRIQIRKLLQKIEISAKQGEELSYITQFLQKITELANQAGGEAPKPTRSNTAVL
                     DDIRLTGGNEQLLALYNQRDELSDYIDRWKDLAARINKRWPNWEILKRLATYASGLPD
                     TEIILAHIKTIEEQRQLLEEPDPVMPLIAGLSQRLRDEINKLSSEYDSNYEQGLKRLF
                     DDENWKQLEPEQQNQLLAEEKLARNDKPKIAVQSTEDILATLDNCDLSMFSDRVAAMP
                     TRFENIARRAAELCEPKVQFIQIPRRTLKTEEDIEAWVEDLKQQLKTALQNGPVMIQ"
     gene            complement(17512..18063)
                     /locus_tag="PK105_03665"
     CDS             complement(17512..18063)
                     /locus_tag="PK105_03665"
                     /inference="COORDINATES: protein motif:HMM:NF020329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HOU60837.1"
                     /translation="MNRIGQLLQSYGKYISIPWRDDAAAAQRVVFCVYNENDERLLRA
                     KIEEFEIVTRDASHQWAMFDLTDTFALWLASQRYAKSYFQKPHLLSTLLPKYLAFITD
                     KFETFLNDSQVGSDCVVALLGVGSLFGFLKVKEVVDKLAPMVKGRLLVFFPGSYENNN
                     YRLLDGYDGWNYLAVPITADKEY"
     gene            complement(18056..18871)
                     /locus_tag="PK105_03670"
     CDS             complement(18056..18871)
                     /locus_tag="PK105_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60838.1"
                     /translation="MAVQGYPMVSMTEKINHLSPFGFSFERGGAHTARTMMLSELRAL
                     LAYVNRPEAEKSDYLKAIVAENCLGKRSGKSRTLTYRHLVDLYSLDRTKLLFRALLYF
                     WYRDIEGQPLLALLCTFARDSIFRSTAPFILQFPEGVTVTRESLEEFLDSQEPDRFSK
                     ATLKSTAQNINATWTQSGHLSGHARKIRTRAHPTAGSVSYALLLGYLTGVRGQALFQT
                     EYAKLLDCSIDQAIELAEEASRKGWVVFKRVSNVIEVLFPNLINQEEMEWLRE"
     gene            complement(18825..19028)
                     /locus_tag="PK105_03675"
     CDS             complement(18825..19028)
                     /locus_tag="PK105_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU60839.1"
                     /translation="MEDRWLSVDEIGAYLGVKRDTVYKWISEKAMPANKVGRLWKFKK
                     DEIDEWVRNGGTRLPNGEHDGKN"
BASE COUNT         5087 a         4638 c         4453 g         5614 t
ORIGIN      
        1 ctgctttacg cagtaacacg caagaggcca gtggcttgga tatattttag caaaagcggc
       61 ggcttatcgg cgcggttgtg gcagaaattg ctgcttccgc gccaaactca aaacagtaag
      121 tgaggctgaa aatgaatagt gattatccag ttttggtaga agaaaaactt ccacggatgc
      181 ggcggtattc aacgctcggt gcgatccgcc gtgagctcat gcggctgtat gaggaagcaa
      241 aaaagcaaaa acaactcgac ccgattgaaa ttcagcgata ccggatgctg tgctttctgc
      301 tatcgagtgc gggcgaactc atgaaggcag agaaacttga tgaaatcgaa cgacgaattt
      361 cagtgcttga acgacaagga gggcgaaaat gaatttggaa aaacggcttg caaaacttga
      421 ggcgcagaac gacggttcgg ctcttgatat ttttgtgcag tacataaatg agcaagatga
      481 aaaaaaggct aatgctttgt acaaaaaata tttgcgagct gatcccaatc aagaaatcga
      541 agaatttctc aaaattattg acagacgcgt aaaaattgcc aagaagcgaa gtgaagaaaa
      601 tgaacggctt atagctaata gcgagtggcg gatagtggag gatcatatct gagagcgctg
      661 cacctgtaaa gagcaacatg aaggaatctt ttcccctttt ttctcctctt tgcgatgtgt
      721 aatgtaagct aaaactcccc cttcggggga aattcagttg acgttaaaca cttgttattc
      781 atcatcatta ttaaactcat caataatacg ttcagcttca tgatcataat gttctgccaa
      841 ttctctcatc gtagcagcaa gtctatgaaa acccatattc tctgctgcat cagctttata
      901 tcggcactga tttgccagat ttctttcttc ctcgccagtc ggaacaatag aatgagctcc
      961 tcttgaattg atgaaaccca gcataaagcc tctacgcaaa tgattaaaat tccgatcgtt
     1021 caaagcttga gcaacaactt ggtgaatcca taatccatta gggtctgcag gtgagttaat
     1081 taaaacctca ccaatatggt tcatcgctac ttctaagtgt cctgatgcct cagttagttc
     1141 tttaaccctt tgtaaccatt gtttgaaatt ctctgggtca aacgtcccat ccttttgcgt
     1201 gcctggaaga attttccatc cctgcagcaa tctccaagca tttaaagcaa cagatttttc
     1261 tttttctgta agctctttag gaggctgggc ctcatttttt ggtcgataaa tcagacgaat
     1321 tatctcacaa aagaattcag gaacattggc cattctgctt tcaagaaact ttggagcagt
     1381 ttcttcgcta tagctttcca ataagggaag aaaagcccac tccaccttaa taagatcttc
     1441 ttctttagcg gaattttctg tctggagatg ctttataatt tcgacgatgt tatggatatc
     1501 catagaagtc tctggatgat cagaagaaga tattgcaagc aaggctctga cgcattgatc
     1561 agtattgagt agctgattct catataacat tctactcaaa caattgatgg cagcataagg
     1621 tcgattatat ttaataagct tttcaatagc gatattcaaa tcaccatcgc actggtaagg
     1681 gatggcctca actcgcgtcc aatattcctg ctcttgctct ttaagccatt ctgaagcgcg
     1741 atcccatatt gtttttatga atggtaagca cgctagaaat tttcctattt gggctggagt
     1801 ccaattggat ttatctaggt tgtcgcacca atctaaacca tataaatatt ggcgctgtaa
     1861 gataaatccc ttgactagat cgtttcgttt cgcatcatca ggatccagaa aatgtggcaa
     1921 aaaagcagca tcaattttaa aatcatcaat tgcagcacat gcaactccta cttttcttgg
     1981 agaatcgaca gattcagcaa agcggatgat gccttcaacc ccattttgat caaagatttc
     2041 cttaatagct gcttctctct taacatctag ttgcttttgc tgctctttcc aatcgctatt
     2101 tttctcaaat aaatcaagat cacgagtagt gaatagaaat ttatagcgga tagaggggca
     2161 tttaggaatc agatgttctg ataccttttc aagtcggata attaattcac caggcaatgc
     2221 ccattgcgca tccgaaaacc gccgatgttt cactgtaaat ttgcgaatgc tatcccaaat
     2281 caagaaccgc tgatcttctg ccagttggag aatcggtggt gagtcaaggc ggttaattat
     2341 ctgattgaaa acagatggag gcaagctatc aagatgctga attaaatctg aaagacgatt
     2401 ggcatcacat tcagcagcat gaacagctag ttctgcataa atagagatct gtttcaaata
     2461 ttcttcttta gttacttcct tgctcccctg atcttttatt gtttttcgcc atactggttt
     2521 ataggaacca gaagaaatcg tatgttgatt aggcaaaagc tgaataatta ggttccagcc
     2581 aatatcaggc cattctttaa gtagtatctg caccgctact ttgcgtttat ctacagaggc
     2641 taatgtttgc gggaaccagg ggagtagaat agtcaccagt gaattggatg gtcgattcga
     2701 ccattgacca cctgggtcat ggctagcaag ttccccaaga ataacacaga tgcgaactag
     2761 atactgttct tcccaggcta atgcttcaag agcccataac aggccagtga gataattcct
     2821 gccaaaaacc ccactacttt cttccgaaaa aagcctataa aaagggcaag gtatttggcg
     2881 aagtgccttc tcaaccgcat taagaaattc acctggatca gcttctgcaa gagctggtaa
     2941 taaatcgtta aggctgcccc ataaaatcca gtcggctcct gagaaaagct gaattaaggc
     3001 taatttgcaa ttttctttag ccttcccgac cgagcaatgg atacacgcat ttgaactact
     3061 acctaaaact gctaagcctt cagagattcc tttccgcatt tcgtgcgaat acaaaaacac
     3121 cttattatga atgcttgcag caaatctctc ctccacgggt aattcaaaag ctggatcgcg
     3181 ctctttaaga atagtaacag ccagcgatat aaaggtatca agatttttat ctagcacacg
     3241 cgatcctagc aggttccaca gttcagttcg attattaatt ttccaaactc catttttaac
     3301 tgataatggt gtattatggt aatgtaataa ttcttgagcc ttttttagcc attcatcata
     3361 gttaatacct agcatctggc taattacatc aatgtctgat tgattttttt cattccaagt
     3421 tcctatcagc acaagtaagg cgataaatgt tgcatcagga tgttgcgccc aatcaatttc
     3481 agaattctct tgagcaactc ttaattcatc tggtattgcc actaaaaatt tctggttgaa
     3541 ggcattctga aggtcttctg gcagtttatc aggagcaatt gttcctgcat tcctattcca
     3601 ttcttgtaca aaagcaacaa tccgaggcca aacccactct acattcagtg gcttaaatag
     3661 agaaatatgg gaatgaagta gagataggac aacaccactc tctttatcaa gatcgactac
     3721 taatagatga aaatgcctta aaaagctata gagtttatct ctttcaacct cattgcctcc
     3781 atttgctgct ttaagatgct tttcgataac ttccagtttt tcagtgctct ttgatggact
     3841 gaatttcgct tgatgcacat gcgtgaaaaa ttcatcagtg ttctttgtat gccttgcctg
     3901 ttcaagaatc cattgtacat tatgtgtatc agtcgcgctt aatggacccg tgattaatgc
     3961 gatggcatcc ttgcctttct taaaattttt agaattgttg taatcattcc atgcagcctg
     4021 tattacctca tcaaaccttc tgtcttcatt tgtgatagta atagtaagct ttatttgcgc
     4081 taagagcttt cgtcgctcgc cagtgtttgc atttttcaca tatacaatca aatcatctgt
     4141 atcaaagcca tcaacttttc cttgaagttt gatctcaata ataggccagc aagggagaca
     4201 gggcgcatac ccacctgaga gcatcaatgt aacaaacatt gattgtactt gagcttcaaa
     4261 gaaagcaccc gcaccaccag tagagatagg agtgcttaat tgtttgcttt ttgccattaa
     4321 tatctccacc acaagcttct ttccctaact gctatgcttt ctcattctat cattgccttc
     4381 ataagggcat ctcgtgcatc tgagtagaat tgaatatcaa tcttggttgc catgtcatct
     4441 gaaagatcaa gaagctggcg ccggcatgca accggcaaca agagagctga agcacctttt
     4501 tcgactgcga tctcagcgat agtgacggag ttgtgaattg gctcgataga tccgcccagg
     4561 ttaatctcac ctacgatgat gagaccgcca cggacacttt tcttcaagag agcagtacag
     4621 agtgcaatga gtgatgccat tccgagtttg gcaccagact tcgatacatc aaaggcccga
     4681 agttgtactg taaattcgtg ctggcgtggg tctttatccc cgacgagttg cacggctcga
     4741 gcatagagat tttgctcagc atagcccatg ctttctttga atgcgggagg tacaggttta
     4801 ttaaggatct tgacgccaga ccccggacct tcattgattt caatacgata aagcccagga
     4861 tgttcttcac ttcctcctgg tgagatggtc cagacttgac ctggttcaag ggggtctcca
     4921 ccgattgtgt tttgactttg cagttctgga gttgaaacaa acttctctac accctcagtc
     4981 cccatgaaat aactgaaatg ggtattgcga aattccgctg caccgatgcg cttttgctgt
     5041 tcctttacac gtcgccttgc ttccatggca atgcgtacag cccactcgac atcttcatcg
     5101 aggatttctc cttcacctgg atataaaagc ttgagcagcc cactggcggt tttattgaca
     5161 gcgttagtat ctcgccctga gagagcaccg ccataatata ctcgattctg caatatgctc
     5221 actctgtttt gattgcgaag ttgactccag cactcggata aaaagtcgct taccaaaccg
     5281 aagtgattgg ttaaaagctc attgccgatt tttggtacat cccatccggg caaaaaggcg
     5341 tggattcggt ccatgaatgc ggtatcatct ctcatttccg gcggtaaagg accaaataaa
     5401 tgtcctatgc gctgctggtg atcaacatcg acttcaaagt tgcctataag aacaatactg
     5461 ccatcagcac gaatgttttc tttaccacgg ctgaattcgc cagattccat atatcctttt
     5521 aagatgttta ccccatcctt ttgatcaaaa gagataccag acacctcatc gaagcatact
     5581 acatcgtaaa ggcacaccaa gcctcgttgg cctgttgagt tattgacgaa catttgggcg
     5641 acagttgcct tgcccccaga aataagatgc gcatagggag aaatttgctg gaaaagatgg
     5701 cttttcccgg ttcctctcgg gccaagctcc actaggttat aatttcgttc aacaaaagga
     5761 accatgcgaa gcaataatgc atcacgctga cgatcagata gaccaagcgg ctcgatacct
     5821 atcgaacgaa ggagtaaatt cttccattcg agagaagtga aagatttacg ggcatcagca
     5881 agaatatcaa gcacatcatg ctttgagagt tgtatttctc tgagcgattc aatgccgaat
     5941 ggccttcctt tattttcttg tgcaatagta gcatcatagc tgagagtgat ctctgcatag
     6001 aaaccaccgg tcagcatgcg ttcgtgtttt ttgaccaatt ctgagcttat ccggacgtca
     6061 ttgagccgca agcttggcaa cgtggcaatg taggaatccg ttcttgcatc caagcgtgca
     6121 gaaataatgt cgataatttt aatctcacca ttctcttttg ctcgtgcttt gaagagctct
     6181 tcttctcctg ctttgacagt tcgcgacatg agctgtcgtt gaacgatgtc aagaccctct
     6241 tcgatttcct cttgattagt gctcgcacaa tagcggccga gcatgaactc gacgacatag
     6301 gtcggaacgg ggaattgccg gctaaaggtc cgaaccaggt cttttcgaac aagatatcct
     6361 tccagctttg tagcagcttt tttatcgaga ttgtccatct cactcatggg ttgcctccgg
     6421 caatgactgt gggtgtctgc gcaatcaaag aaccattctc atcgatgagt acaacaattg
     6481 ctgcttggcc ttccatatcc tcgtcttcga cgatcacgga agttgttcca ttgtctttga
     6541 gcgttttttt gtctacaaca acactggaag atgcatcacc agcatggctt cgaatgtcga
     6601 ttgccaaacc tgagaaatat ccttctacag tcacggtaca gcggagccct ttccatacca
     6661 caccggcaat ttttaccgat gtggcagttt gctcagatgc ttcttgggtt accagtaaat
     6721 gcagagtgag gcattcttgc aagctaaggc caccatgggt gtactcttcg cctctcttaa
     6781 aacagctcac tccatcagcg agtgcaaagt attggttagg gttccaatac catgggaaaa
     6841 gccgcacatc agaagtagct ccaggtttta atgccgcgca tcgaccccac ttgctatcta
     6901 ccaaggcgct tggaaggttg attttgggta atccccctgg taacaagagc cacccatgat
     6961 ctgtcacaac ccggactctc ttccatcctg cagccaaaag ctccgtaata cggtctgtga
     7021 tctccaaaat taaggaatcg atatatttgg taagtttcca tccacgcgca tggccttcat
     7081 ggtcaatgtc cccaaactcg caccaagcca tgccctggct gtcgcctgca ttagagttct
     7141 ccaggattgc ccaccctgaa tcgcgcagta gttttttaag attgtagcca cccttcaatg
     7201 actgtccggt ttctataaca gatggttcaa aatctgtact gctatcttct ccgaagattc
     7261 tatctcgtac cggacttacc gctgctttgc cagtttctgt gacgctcggc agggctgacc
     7321 acgcaagttc ttccgtaact ttatagttac gtgcttctaa agtactaatc agacgctttc
     7381 ccatatcaaa acgcagccca tccacgaaga gaatgcaatc accggggctg aaagaaactt
     7441 tctttggcgt cagacaggta ccccctggat aggaggtgct gtcaacaagg ttttggagat
     7501 agcgagcgga ttcttctatc catggcaggt aaatagcccg aatagcagca atcaccgctt
     7561 caatgtcttt tgaactctcg acctgggcca gggccttgag tacagcatca tcagcttgcc
     7621 atccgtgact gcgatatcca ctaatgaggt cgtccaacga acctgcagca aggctgtttt
     7681 gggtgatttc agcgagaatt gctaagtatt gcagggcgaa ggccaaagga gcttccccaa
     7741 gttcggccca cactaaagag cgcctgcctc catgcttttc ttcaagcttc aagagttttt
     7801 tccgcgcctc atgcgcagtc gtttgggcaa gggctaaaag atcatattga aggctctttt
     7861 cctgatcatc gttccactgc ggccagccac aagccgaatc cgaatctgtg tacaaatccc
     7921 attgaggtgg agtacatttt ctaattttat ttgggatatt agcgtagcgc tttgatgcct
     7981 cgcagtagcg ttcccaaacg gcatgccatg ggccttgatg gttcgcaagc ttggtgattc
     8041 cagtcaagat gccttccttt tgaggatggt aggctagttg agactcacag acttcaacaa
     8101 aggcttccca ttcattctta tcacgatttc tctgaaagac ttccccttgg tcgagccatt
     8161 gaagcaaatc acgaattagg tcgttacccg aaagcaaagt attgaagtag tccttgtcca
     8221 gatgcttagc ctgaagcaaa gagacatcct cgtcgagaag ccgatatagc gaaagctgca
     8281 tagctttttt ggtatcgttg tcttgtgcga catctaaccc aagcccaccc tgatttgatt
     8341 ttaagtatgc caaaatagtc caatctttcg aattgatttg tgaccagatc acaccgcgaa
     8401 attgcaattc agcaagggct ttaaggtaat caggacaggt ttctacagcc cgaaggtccc
     8461 gtctgctaac acctggaaga tagagaacag gaataatggt attggtagat cgcattgatg
     8521 aacggatttt ggcgacaata gagcttaaat ctgcatttgc ttgagtaatg tgcggatcat
     8581 tgattcctgc aattatacat cgtagccaga ttgccgggcc gatctctttt tcaggctcgt
     8641 agtcgccaag cactaacaat tctggcattt ccgattgcag acgcgatatt gcggtttccc
     8701 attgccggtc gtgatccggc cacaaaatac aagcaggggg cacctgaatt tctggattgt
     8761 agacagcagc tgagcgaata tcttttagca aatgatcaag cactctcatg cttcatatcc
     8821 ttataattta aaaagctgaa ctacttgttg aacgagcttt tctgttcgtg aatcgatctg
     8881 ggcaacactc cacctctcag cagaagccaa gtcctcgtta agcttgagtc cgttcttata
     8941 accaaccgca tgaccatgtc gatcggtacg atttcgctta tcttcgaagc tcttattgcc
     9001 aagtgcactg ttgaatccag aaatggtaag attgcctagc ttatgaacat gcgtctgttg
     9061 aatttctttt gccttttctt tattaccatc ggcaatcatg tttatccaag actgagggat
     9121 attctctccc tgtggaaaaa tatgttcgat ggtccacaca aattgattgt tttcaatacg
     9181 ccatagatca acccaactct ctttggtcat ggactgctcg gcgagtgcac aaagaataaa
     9241 gcgggttacc cctgaatttt cctcataaat tggacctttg agcttgcttt cgaatacctg
     9301 atcagttgca gaaactgcta ccagctgctg tctcattgat tggagaatag catcagcacg
     9361 cagctcacct agcttgtcaa tcaatgtcat gaacagccga gtaagatctc gcgttggtgg
     9421 tgtatcggtt aagttgcgac gaacgaagaa gcggaccagc agttcaatta tagattggag
     9481 gtgagcattc gtaagctcga gctcttcctt ttttgcaagt aaatataaaa ggagtaaata
     9541 tgatggagcg ccctgaatgc gttccaaatc cttgagcgat ttttcgagtt ctcggtacgt
     9601 gtcatccttg ttgcgagaaa gcatcagtga atagagtctt ccagaaatac tgatcttctg
     9661 taaaaaatcc ttcgcgtcat aatcgataag cttttcatag atttgaataa ggttggaccg
     9721 ggttgcaact ggaacctggt ggaccgcttt aagttcgttt ttaaaagcat tgtaatactg
     9781 ccgaaagaag cgctcctgga tcacatagtc atcacccagg tactgcaaga gacgattcca
     9841 gtgcgtaaaa taatagtcta tcttgcctgg ttcaagggat tccagacgtg caagaagctt
     9901 gttttttata agatcgattg cggtaagtgg catgcctcga ttgttgagcg actcgaacag
     9961 ggtgtacgcg tcagcatgac ttgctacttc aatcttgacc aggtatgtct ggttgacttt
    10021 atcaaggaat tctatgatgg tttccagctg gttacttgta ccattggcca tttcatcgat
    10081 acgatcctgg aaataccgat aagcccgata gatcttacga ttacctgcgt atgcgggggc
    10141 atcatattca ctgatgactc cgatttcagc gagtacagca cgataatcgg cgttattatt
    10201 attctggatc tgggggatga ggcgaatctg atcatcgcct tttttcagaa ccaactttcg
    10261 ctttaaattg atcaattcga ttcgttggtc atcatcaaga tcgttttgat gattttttag
    10321 ggtcttgtag accgcagcaa agagcaatga gagcgttgtc agacgttgtt gtccatcaac
    10381 gagttcaaga ttttgcgctg aaagcgcatc aatgctttgg ttaatgcaga tgattgagcc
    10441 aaggaaatag ccagactcgt tctcacgcag atcatcgaaa agattttccc actgattttt
    10501 gccccatgtg tattcgcgtt ggtatcgtgg gatgacgtac actacgttcg cttcgatatc
    10561 aaagagctgt gagacggggt aattgttgac tgatttgatc attttgcagc ctcccgtgct
    10621 gtgcgttttt cagccagcgt gaggtgatgg tcgttgatgc gatcgccttc gtgcccgccg
    10681 tattgtggac caagatgata ccatggtgca gatttcacat cattgccgcg atcttttttc
    10741 caatcgatat ttggcttatc tcgcaggatt ccagcgccct ttttctgcac atcgggcaca
    10801 gtcatgaagg ggcggatatt gaggcgtacg ccgtcgttga ggtcgggatc ccatccgatg
    10861 ggttgttttt cgataggttt ccagcgcaca aagatgtcgt agggtgcttc gcctgcaagg
    10921 ataagctcca gctttttctt gagagcttca gcggcagcaa gtcgctcttg cgcgccatcg
    10981 acaccactgg cgatgtcctg cttctggcgg ttgatccaat cgcccacata ggtgtagata
    11041 agggtctcca gattcttgcg gtcgagcttg tggtaattga ccagcacaga aaatccatct
    11101 ggcaagccgt cccaaatatg ccagatgaag gggcggtgat gaaagagctt gcagtgctgg
    11161 gtgaagaact tatctcttag ccaggattcg agagtcttac cgacatgatc agcgttcttg
    11221 agcagcgtcg ccaggatatc gttggaccag gcatcgccat aggaggcggc gagtaggttg
    11281 aggagacggt cggccgcagg tgcttcaccg cgtactggtg gaatgcagac aatgccatcc
    11341 tgatcggcaa agggcagtag ttccttgcag cgctccaccc atttgtgctg ctcatcggcc
    11401 agctccattt ctggatcgag ctcggctggc cagcggtagc cgagcagacg agcaacggca
    11461 acttgcagta ccgtagcgtc ggtgcgcagc ggcccatttg aagtcccttt cttttgttcg
    11521 tcccaaatca ccgaaccgca ggggtggccg tggaagatcc attgggtggg gtcgtcggtg
    11581 tagggttctg tgggcaaagt ttcgagggat gttttcctcc tccagacctc aaaatcaaca
    11641 agtgcctttt caaagtaccg aggctcaaca ctaattttct tattaaacga tcgaatcgat
    11701 gaaacaaaat cgtccgatga acagtacgcc catagtgcac ataaatcttc gggtttttta
    11761 ggaatcaaga ccccaatatt tgcggaatat ttattcccag tgaaaatagt tgcataaagt
    11821 ctacccatta gattcactgc aacaccagtt ttgccccagt ttggctttcc ccttcgccag
    11881 ctctggattg catgatttag gcttttaagt tcttcggcga tgcgagctat cgtaccacat
    11941 tcactttccc agaaaatcac ttgttcgcgt ccgccaaatg ggaccgtatg cgaagtatca
    12001 gactgaaagt actcccaagc attatttggt agctcaattt cccagaaata tttaacaaac
    12061 cagtttactt ctcccgttga aaggccgcga agcgctttgg catattcgcc taatggcttt
    12121 tctctggagt taggagaaaa atttattact gtatgcgtgg agttttgtag ctctatctga
    12181 tttaaagaat tagtgaattt tctcctcaac ccgtcagctt ttcgatcaac gccactttca
    12241 tcacttacat caattgtata aaaatttgtt tttagtttct gattactcgt aattccaagc
    12301 atgctctgca ccacttcacc tgaaattgtg ttaaacgccc cagcaccaag cctagccatt
    12361 atcttccatg aagtacttgt aagcatttcg ttacgaaaat gcctttccct attctggaat
    12421 gtccagccag aagtagtaac aaaacaagca aggccacctg tttcagttaa actgcataac
    12481 cttttaacaa aaacaaatgc caagtcgttt tttgctttcg cataattttt ttcaacaaaa
    12541 atacgaagcg tttcgctaaa tctattaatt gcaagatatg gtacattggt cattgtgact
    12601 gaatatttct gcccaagtat tcttgcagct ttgttcaatc cctttgcaac aatacccatt
    12661 tctgcctttt catcatcttt ctcccccact aacgcttttg ttagcagagg ttcgacttct
    12721 tcccacctta attcaaacag cgacccctta cctaaacttg cttccggatt gatcaaactg
    12781 ccgagtactg gtgcgtcttt aaactgtttg taaagttctt cgagagagag cagaagttta
    12841 gtgttgttcc cagccagcgc caaccagtcc tccttcttag cactgatcga caaaccggaa
    12901 caggcgacat tcaattccgg taacacccgg tagccaccag cgttcggata tttccatgca
    12961 gttagcgcaa gtgcaaaggt ggccagttcc acacagcgtt gatccagttc cagtccgtgg
    13021 atgttctcgc taagcactcg gtttactgcc tctctggccg atagcccttc caactctatg
    13081 cgcatcggca ccagcataag aaaggcggcc accaggaaat gaccggagcc acagcatggg
    13141 tcgagcactt tgagttgaga caggttatcg ggccaggatt cgaaggtgcc agccgccggt
    13201 atccaggcac cattttcctg cctcacaaag cgcagatagt ccagcggcat cccggggatg
    13261 gcggctctgc gacgcagttc ctcttcgctg ctcgcgtttt taaggtcgtg ctcggtaagc
    13321 cgccttgtag cccaccaggc tcctagagaa ttgtcgagca ggaagctcac catgtaaggc
    13381 tcggtaaaga gttgagtgac ggcaggcagc tcgcgggagc caattttgac ttcagagtcg
    13441 tctatctctt tcttcttttt ggcttgccag aattgataaa cccaaccgag actgtcagag
    13501 gcagtgaaga cctccggtgg taagtcggct aacagacgct ctagtttctg ctgatgctcg
    13561 ggtggtaagc ttaattggaa aacgggtgaa tcgattcgga agatctgggg cagcatgcgc
    13621 gaggcaaatc gggcggctag ttcccaccca ttcgttgcgc cttcatcaac agcaaggtct
    13681 tcacattctt cgagagttac cggcactggc tcattcgggt caggatacat cagtaagttg
    13741 ttctcggcca gaaaccgggc aaataacatg cggtgccagt gctcgtaagc gacctcttcg
    13801 ataaggcggt ccatcgtctg tgcttcgctg ccatttaagg aatctccaag ctgacggccg
    13861 tgagcacgca gcctgcggcg tagcgctctc tcttgttcgc tcaggtgggc aaagggctcg
    13921 gcttccccca cagcaagttg ttccagcgca gcgcgggctg cgtcctcagc aatatcgcgc
    13981 gcatttctga tggtacgttc aagcaggttt cttaatgttt tgttcagtgg ctgcatgtgc
    14041 tatttctcct ggagcttgct tactgaatca ttaccggccc gttttgcagg gcagttttga
    14101 gttgttgttt taagtcctcg acccaggctt cgatatcctc ctcggtcttc agcgttcgcc
    14161 gcggaatttg aatgaactga accttgggct cacacaattc agcggcacgt cgggcgatat
    14221 tctcgaaacg agtgggcatg gccgcaaccc ggtcggaaaa cattgaaaga tcacagttgt
    14281 ctagggtggc gaggatgtct tctgtggatt gcacagcgat cttaggcttg tcattacggg
    14341 ccagtttttc ttcagctaaa agttgatttt gttgctccgg ctccaattgc ttccagttct
    14401 catcatcgaa taagcgtttg aggccttgct cataattaga gtcatattcg ctactgagct
    14461 tgttgatctc atctcgcaat cgctgggata ggccagcgat cagcggcatt acaggatcgg
    14521 gttcttcgag aagctgccgc tgttcttcaa ttgtttttat atgagccaaa attatctcag
    14581 tatccggaag cccagatgcg taggtcgcca gtcgctttaa gatttcccaa tttggccagc
    14641 gtttgttaat gcgagcagca agatccttcc aacggtcgat gtaatcactc agctcgtccc
    14701 gctgattata gagagccagt agttgctcat tacctccagt gaggcggata tcgtccagga
    14761 ccgcggtgtt tgatcgagta ggtttcggtg cctccccgcc tgcctggttg gcgagctctg
    14821 taattttttg taggaactgc gttatatagc tcagttcttc tccttgtttg gctgagatct
    14881 cgatcttttg caggagctta cggatctgga tacgttgggc ggtggtgata gtagcagatt
    14941 ctaccttgaa gactactttt ccaattgctt tgcgctccag ctctttgggg tcgagtatta
    15001 tgacatgctc atcttgtgcc cggattaatc cagcaaccaa tagaacttgc atggcgccgt
    15061 ctatcgcatc acgtggccat ccatagggtg gcgattcgaa ttttgtgcgg atatctgcgc
    15121 cttttttgcc accggcaatg aacgcgagaa tggctttgca aacgggatgt ttggctggtt
    15181 catcttcgta atcgacagca ttgagagcgt cgggagcgcc agtcttggct ttttcgtaca
    15241 cctttgccca acccgtgtgg tctgcaatct gaaattgtgg ataaagcctt tgcaatgcgt
    15301 ttgaggaggc ttccaatatc atttcctgca aatcgttccc agctatttca ttgcctccag
    15361 cttggaaaac tcgggcagca gaaaaggatt cttcgagcag ttccccgatt tttttctcgg
    15421 cggactgctt ggtggtctcc atcgcagctc gagcttcgat cccttctggg gtattgggaa
    15481 ctccacgctt gtccagtgtt gcgctcgcgg ctttgtaatc aatgaggtga tgccggaggt
    15541 catcagcgga acgtttaggg atgaacacaa aaaccgttgg ggattggata cccgcctgcc
    15601 gggcatcggc tcgcactgaa ttctcgtcaa tgctccagcc atcgcgaacc caaagataaa
    15661 tgcgctggtc agcgtcgtgg ggaagttggg catcaaataa tggcgaaatt tcacggatga
    15721 cctttgaatt tccttgggtc agcgagagct tacccacaat ctcgttaaat ttctttcgga
    15781 tgcgctcgtc tcgttcagcc tcgatacggt gtgtctcgtt ggacaaggca gagcgctggc
    15841 tcaaaaattc gtcgttccag gctgcgctct cttcggtctg gatgcggtat tcatcgccga
    15901 ctttcatgag aagctcacat gtatcgagca agctgggcaa tttgctgcgt agactgctac
    15961 taccgtcaga aaggttttcg accaataggt cggcgagggt gtcgatcgta gctcgaatgc
    16021 caatttcagt attctgacta ccaagcttat tgatgaggaa tactagtcca caagcacgag
    16081 ccatgagcct ttcatcttca gatcctctgc tccaaatcat ggttttctcg tggactttgc
    16141 ggggaagaat tcgtgactga agaagcttgt ctgcagaatc gaaataaagg tagtcagctg
    16201 gtacaacgtt ccccaaaggc ttatctaagt tggtttgaat gaccttgtga accatgctta
    16261 gctgattccg tagctggcta tcagttccag tctgatcgag tacccttaat gtgttttccc
    16321 aaaaacgccg acgcaccggc aaaaggggat aatcttggag ataattggag atgtcctcct
    16381 gccgatgacc gatggtcgtg cctgccagat gccgcgatat ctcaccaaga ttcaattgca
    16441 taatccgctc aatgggcgct ttggcttctg gtttcttggc cagaatgact tgacgaatga
    16501 ctgcatccac atcggcatcg gagagttcaa ggcggatagt aaaacgtcct tcgagtttct
    16561 tcaggttact agttccagtt accgcagttt gaccggtgcc gataaataga agtttgccgc
    16621 caaaattctt gcaacaggct tctaccacct cctgcacgtc gacggaacgt tgactgtctt
    16681 cgccgataaa ctgctggacc tcgtcgagta ccactaaggt cagagggaat ttgccatcct
    16741 tcgtcaacgc ctgccgaatg gctttgatca tatcatcgct ggaaatatcc tgaacgtagg
    16801 ggtaaagatt gttcaaggtt tcgacacaag aagaagatga tgaaaagaga ttgggctttg
    16861 cctgaacgag tgcatcatgg aggccttcag caacataaaa attatccagc tcttcgttcc
    16921 agtcgaaact tttctgctct accagagtgc gtacttgatc atagatacct tcatgtttga
    16981 gccacattac gaagcgggca acagggtact gctcgggcaa tcctgcggat ttgaaaatga
    17041 tacgtagtaa tgccagccgg acactaccat ttgcgctggc acccaaggta ccggaggcag
    17101 catgcagacc cccatgacgc ttgccttgaa tactcagttc tttgaggaga tcgcgaatat
    17161 tttgcggcag attggctatg ccgcgagccg tggctccatc atcgaaaaca gtatcgaccc
    17221 aaagtgcacg aagcattttt acgagatgag atttgcctga tccgtaaaaa ccgctgaccc
    17281 agacagctgg ttgctgtgct tggttgatat ttctcagata ggtgtcgaga atgtggtata
    17341 agcctttttc gtattggcct tcacagacaa aggtttccaa ttcgtatcga agcactgcca
    17401 aagcctgact ggtgttctcg tcgttgacgc ttgccacacc ctcgttgacc agctttcggg
    17461 aggatggatc tttttgatag atatcacgat tattcatcat aaacctcttg ttcaatattc
    17521 cttatccgca gttataggta cagcgagata gttccatccg tcatagccat ccagcagcct
    17581 gtaattgttg ttttcatagc tgcccgggaa aaagaccagt agccgtcctt tgaccatcgg
    17641 ggccaacttg tcgacgacct ctttcacttt cagaaatccg aaaagcgaac cgacgcctag
    17701 aagtgccacg acgcaatcgg acccaacttg gctatcgttg aggaaagtct caaacttatc
    17761 ggttatgaat gcaaggtatt tcggaagcag tgtggagagc agatgcggct tctggaagta
    17821 gctcttggcg tatcgttgcg atgcgagcca taaggcaaag gtgtctgtca gatcgaacat
    17881 ggcccactga tggctcgcgt ctcgtgtgac aatttcaaat tcctcgatct tagcccgcaa
    17941 caagcgttca tcgttttcgt tataaacgca gaaaaccaca cgctgcgcag cagcggcatc
    18001 atcgcgccat ggaatggaaa tgtattttcc gtatgattgt aatagttgtc caattctatt
    18061 cacggagcca ctccatttct tcttgattga taaggttcgg aaatagaacc tcgatgacgt
    18121 tacttactcg tttgaacacg acccagcctt tccgagaagc ctcttcggcg agttcaatag
    18181 cttggtcgat agagcaatcg agcaatttgg catattctgt ttggaaaaga gcttgccctc
    18241 gtacacctgt gagataacca agaagcaaag cgtaagagac gctaccagct gtaggatgag
    18301 cgcgagtgcg tatttttcga gcatgacctg agagatgtcc agactgagtc catgttgcat
    18361 ttatgttttg cgcggttgat tttaaagtcg ccttgctaaa gcggtctggt tcctgggagt
    18421 caaggaactc ctcaagagac tcacgagtaa ccgttacgcc ttccggaaat tgtagaataa
    18481 agggcgccgt agagcggaaa atggagtccc gagcgaaagt acagagcagc gcaagcaatg
    18541 gctgtccctc aatgtcacga taccaaaaat agagaagagc gcggaagagt agctttgtgc
    18601 gatcaagcga atacagatcg acgagatggc gataggtaag cgtacgactt ttccccgatc
    18661 gctttcccag acagttctcc gctactatcg cctttaggta gtcgctcttt tcagcctcgg
    18721 gccggttgac ataggctaat aaagctctga gttctgaaag catcattgtg cgagcggtat
    18781 gagcgcctcc gcgttcgaaa ctgaatccga acggggataa atgattaatt ttttccgtca
    18841 tgctcaccat tgggtaacct tgtaccgcca ttgcgaaccc actcgtcgat ttcatctttc
    18901 ttaaatttcc agagacgacc gactttattg gcaggcatag ctttttcgct gatccacttg
    18961 tagaccgtat cccgctttac gccgagataa gctccaatct catctacaga tagccatctg
    19021 tcttccattg ttgagactcc aaaaggatag atatattctc tcttgcatgc aatccaaaat
    19081 gaccaaggtc taattgcgtt ttctttctca tatggccttt tttcaatctc aatagtataa
    19141 ggctaagagt ctctttggca gttgtcaagc caatatatgt cattatagtt gagtttgttt
    19201 tgatagaaat ggcttttccc ctctatagcc ttcttattat aatttttaga tcgaactttg
    19261 aaatttcacc gcctaatgcg gaagtaatgt gctgactggt tataccttca gataaaacag
    19321 aaattccttt attcagaaga aaagatgcgg caaaatgtaa aataccaaga agagcgcgga
    19381 tgaatagagg agaatagctc caaggagtat gtcctctttt agaggccttt attttttggg
    19441 gccacaacct ttgctggtgg cagttaattc accgccgtga ggcagcaaga atagtcgagg
    19501 gcgctggtaa aagttggcac ccttgctttt acaaagagga catgagcctc aatataatca
    19561 atattatttc tgaacgagat tgcaaaatga aagcatgctt acattatata ttatatagac
    19621 attatatcta ttaaataacg cctctattta tcgcttgttg actatttgta gaatagatgt
    19681 ttttcaaatc acgaataatg ctgataataa aggctatttc actactatgc cgtgggtgtt
    19741 caagtcccac tgccggcaat ttttcttata gagcctttcg gttattgatg cg
//
LOCUS       DAOOWY010000028        19677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2758_length_19677_cov_11.701305, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000028 DAOOWY010000000
VERSION     DAOOWY010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 19677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19677
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2758_length_19677_cov_11.701305"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..394
                     /gene="rplS"
                     /locus_tag="PK105_03680"
     CDS             23..394
                     /gene="rplS"
                     /locus_tag="PK105_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOU60840.1"
                     /translation="MDLIQQIQAEQIRTDLSDFRVGDTVRVSFKIVEGKSERIQAYEG
                     LVIAIKNAGVGKTFTVRKNSYGVGVERVFPFNSPRVEKVEIIKAGKVRRAKLYYIRSK
                     VGKKAKVKTLVGGRKSQKQEA"
     gene            548..934
                     /locus_tag="PK105_03685"
     CDS             548..934
                     /locus_tag="PK105_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOU60841.1"
                     /translation="MLMSKAEGDKWKRKGREEEIMAGRYLESIGWNVLARNFRSRRGE
                     IDIVATRDNILSFIEVKSANQITERDLEHVIGAKKRNSIIETSKLFLVINRKYKQYRI
                     RYDVMLIQESACLRHIEGAFAEHDGE"
     gene            924..1145
                     /locus_tag="PK105_03690"
     CDS             924..1145
                     /locus_tag="PK105_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60842.1"
                     /translation="MENNKVSRDIPAWAGSTPDDLLYSDYDITEEMKATNWKRLYEIK
                     HKLVDIGYLDGAIASIAEMVSNTIRNKPK"
     gene            1142..1621
                     /locus_tag="PK105_03695"
     CDS             1142..1621
                     /locus_tag="PK105_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60843.1"
                     /translation="MNSEPNANKQPNNPPKHERPGKRIGSQHFRKHRSTRAGAQKTAD
                     RNQENHARMGEPSVESSRQESKICKVCGKPIFDLAGAIASREDGEPIHFDCAIEILSK
                     EENLAPDEKLIYIGSGFFAVCVQSSSDKLEIRRKIRWEISGTQHPWRKPMIFTPNLP"
     gene            complement(1688..2968)
                     /gene="serS"
                     /locus_tag="PK105_03700"
     CDS             complement(1688..2968)
                     /gene="serS"
                     /locus_tag="PK105_03700"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOU60844.1"
                     /translation="MLDIRFIKENLEAVRQNIRDRYMDADVDLLVSLYDKKNALLREL
                     EESRRLRNENAQAMKSNLSKEERAIRIEEGKRLKEIIARLEAELQEIERAFAEELAKI
                     PNMAHPKAPRGKEDKDNLEVKRVGSPTQFNFPPKDHVELGQSLDILDFDTATKVSGTK
                     FYYLKNEGTILELALVRYALDMLSKHGFTLMTTPDIAKSQILEGIGFIPRGPESNVYT
                     LEGEEACLIGTAEITLGGYHSDSVLEKSQLPIRYAGVSHCFRREAGAAGQFSKGLYRV
                     HQFTKVEMFVYCLPEDSELIHEELRSIEEEIFSSLDIPFRVVDTCTGDLGAPAYRKWD
                     LEAWMPGRNNGEWGEITSTSNCTDYQARRLNVRYRDKDGKNRFVHMLNGTAIACSRAI
                     IAILENNQQADGSVMIPKALAPYCGFDLIRPNRT"
     gene            3068..4126
                     /gene="prfA"
                     /locus_tag="PK105_03705"
     CDS             3068..4126
                     /gene="prfA"
                     /locus_tag="PK105_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOU60845.1"
                     /translation="MEQLRGRLAEIDGLVSNSDILRDKNNYRNLMKERAKISEILQEF
                     DEMQRLNSSILETEELIRETKDEELRELAEQEILELKKQYALAEERLETLLAPRDPMA
                     DKSVIMEIRAGTGGEEAALFAADLYRMYSRYADGRGWNVEIMSSNETELGGIKEIIFS
                     ISGDAAYERLRLESGVHRVQRVPVTEGSGRIHTSTVTVAVLPEIEETEIEIRPEDLRI
                     DVMRSSGPGGQSVNTTDSAVRITHTLSGIVVHCQDEKSQIKNKAKAMRILRARLFEIE
                     DKKRQAERSAERRNQVGTGDRSERIRTYNFPQNRLTDHRIDLTLYKLDLIMEGDLDEL
                     LDALIAWDREQALMAEER"
     gene            4132..5001
                     /gene="prmC"
                     /locus_tag="PK105_03710"
     CDS             4132..5001
                     /gene="prmC"
                     /locus_tag="PK105_03710"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOU60846.1"
                     /translation="MNYRELILNESSSFSDSDSPFLDAVLLMAHSLGITKEKLFSMLS
                     ERVDSVPQSFFDMTERRRNGESIAHIRGFREFYGREFLVNSDVLSPRQDTEVLVEAVL
                     ESSKLFEPSAGINGSLDVVREISILDLCTGCGAVAISLAAEMPNHKVTASDISSAALD
                     LAKKNAQRLLPAQSLIFIESDLFSEIDGKFEIIAANPPYVPHYQAEQLIAKGWKDPLI
                     SLDGGIDGMEYIRKIIMEGYEYLSHNGVLLLEMDPHQASESLALFGEMGYIDLKTWKD
                     LTGSIRVVGGRHG"
     gene            4994..7225
                     /locus_tag="PK105_03715"
     CDS             4994..7225
                     /locus_tag="PK105_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOU60847.1"
                     /translation="MDEFVNEFSSKIEHRFNDEEKNLLINALDHAHSVHGERKRASGE
                     PAYYHDIRVAEILLDLGMDLDSILAGLLHDTIEERISGFVQDANLDRNIEGKPANREK
                     SALEESRISPRGIKQTTKGEHALKKKSSNALSSLLAAPDPFIEERFGPQTAVIVAGVN
                     RLSSVKAKNKTVHAAETMRKMLFALTSDIRVILVKLADKLDSMRTLKYLPEDRQKEIA
                     TECIDIYAPLADRLGISWLKDELEDLSLKALNREVFDQIKEIVNSRKHERQDFLKKVT
                     DKILSTAAKEGIDVEVSARAKHFYSIYQKMRRRGKGAEELYDLLGIRIICNGINDCYA
                     LLGLVHRLWKPIDGRFKDYIAMPKANGYQSLHTTVIAYGGQLLEIQIRTREMHLVAEY
                     GVASHWLYKKGTGAEIPKLEDLPIMNKLRQWNQFIAEGDSYLEEIKQEFLGDSIFVFT
                     PKGDIIELPSGSTPIDFAFAIHSDVGAHCLGAKINGAIAPLDSELRNTQIVEIITSPN
                     ARPNLNWLKIARTSKARSKIRQLLVQTGQAVAIDKHIVAARKSEKAAQETVQARVHGA
                     DRSQIEARRGEFPSVVEFHSLKPGLVLRSEKVGVSVGGMRNLMIRIAGCCKPVTGDRI
                     VGYVSRGRGIIVHREDCRSIASIADFEERRIDVKWEDEAINTTARYRVTTKKSYDIFS
                     EIESTVRRCSGRLKEGKLGERGDGTLSGFFILEVDSREGLKRVAKALRTLPSILSIEE
                     EPI"
     gene            complement(7235..8659)
                     /locus_tag="PK105_03720"
     CDS             complement(7235..8659)
                     /locus_tag="PK105_03720"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU60848.1"
                     /translation="MILFLILSKGSSASAQYQIKTLANGMTVCLNPIEYEEDISIILA
                     YRAGANSQTASTAGLFKLLEYVLFNGPAANPGIPEPAAAIDVLEAEDIRGGLGIDRFE
                     FGISFKKENLVQAFDTLLYLFSQERREFIFAQKDGIELARQSILNLIQNETSDNDLVS
                     SMAINKKLYAKAPYRIDILGSENVLEKADDAQLKNIASSWLVPNNACLSIAGAFDPNQ
                     VLSLIEARFGLLQRAKNPWPANLAPFPRPGVTKPIFLVFPDSDISADHMKIEMRYRGP
                     DPKDTTSYFAALMLQQLANEPTSRFQMAVRKSMPKGSEPENIRIDYVPNTDSSWISVL
                     SIVPAGKKPAETVFAFKEAVRGTELYMVKANPSYFSAADYDRACQALLEEIMAIQGDA
                     MSSACHMATLWSWGIPSLIFQESDFIMKTRQKNISQFVDTYIQKNLEVVMVRLNPDLY
                     EVNKKSFSSYGFETVSTKNAFWWR"
     gene            complement(8721..9080)
                     /locus_tag="PK105_03725"
     CDS             complement(8721..9080)
                     /locus_tag="PK105_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU60849.1"
                     /translation="MDYVRYCFASNLRLRRAILQMSQEELAHLANLSPGFIANIETGR
                     NFPSSKGILKISSALKVEPWKLFLDPQKQDMFFTRDEVSHWLEDSRKRLFGDFSQGEY
                     HTEDDSEIDSNEITPQD"
     gene            9234..10652
                     /locus_tag="PK105_03730"
     CDS             9234..10652
                     /locus_tag="PK105_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOU60850.1"
                     /translation="MFIREIYIKRRRAFLDAIRDRGITSGKVLLLANENCPRNYPENY
                     YPFRQDSSWLYLIGLNIPSLACTLDIESGGNCLYADEPGLEEIIWTGVLPSPSELAYS
                     AGIDKVLPTSVLSNTVGRALKQREPVFILPPYRDRQIARFLDLFAVSVSNLEELIYRH
                     IDPRIILALVSVREIKEPEELSELERAVSITKIIHEELMHELKAGWTEKDAAGFVSSR
                     ASAQGCELSFATIATCRASVLHNSPTEHVTTEHDTFLLDAGVETTLGYAGDLTTTFPV
                     GPSFSHQSREIYQVLYETLQTATNALAPGVRFIDVHKIACLTITKGLLSLGLMKGDPV
                     EAVESGAHALFFPHGLGHQIGLDVHDMEGLGEDYVGYGDELKRSEQFGLSSLRLAKTL
                     KPGIVHSVEPGIYFIPQLIQKWRAERICESFLNYDMIEKWMPIGGMRVEEDWCITDKG
                     ARRLGPAFDRSIEAIQKVRANR"
     gene            10649..10876
                     /locus_tag="PK105_03735"
     CDS             10649..10876
                     /locus_tag="PK105_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60851.1"
                     /translation="MKTVFVSAFLEDSIVVKTMLESAGIPAEVLSDQMLDINPFYSIE
                     PKGAKVIVPDSSEEDAKAIVADYREHKQSNK"
     gene            10916..12130
                     /locus_tag="PK105_03740"
     CDS             10916..12130
                     /locus_tag="PK105_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU60852.1"
                     /translation="MKRKLFVPITITLASLLLIGLLIYSIQRHIAGRNFESVNDALSS
                     IIEIQAYAFYNGCLSNNQTYNLVIQGEFAEANALVQKATDSFSLIDLAEIVEGKPPSD
                     SFDIIMDGELLRIECPIRNDDETAIVPNMIGVAIIQAQRLLNTVSAKDFRIDLAHGRK
                     TTYGIPISANFTGNRLWDSIIIALLTGFVAIFIFQHFYRNANTFLDTRGLESIIYLFE
                     QTERISASHSRNVAMLALFVGKNLGFRGKRLKDLYIAALLHDIGKIGIPNNILTKNGK
                     LDPCEFVQMRQHPAISARILRSFKEFERLSPIVLYHHEREDGSGYPEGLTGRDIPFES
                     KIIAVVDVFEALIGERPYRDPICPLSAFQKMRSMPLDQKIVSLLMDHYGELDGFRPPR
                     WALSYSPWMLTS"
     gene            complement(12125..13222)
                     /locus_tag="PK105_03745"
     CDS             complement(12125..13222)
                     /locus_tag="PK105_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU60853.1"
                     /translation="MTEIYSRLEEAHLLFFEYGITALDAYLDEKPRPLKFLAVQCSLV
                     ELARVFDILEYPNLPYADASLCDEVRFICTEDALEENLGSPWMNFKRSPSTGVYYDKG
                     DIYNSLKARNIQPSSSEYSEIERSLFDFTLLATLSELPPKTIGSIALPPSPSVLFQRD
                     LLIGILSGPYPVSGFEVLKQSGFIAREWPEIALLDNVDHSKDFHPEGNGWNHTMQTFS
                     YRKTRDITLSLALLLHDIGKSESKSQKGHRFDRHAELGAKRAEKFLSRLGFSEEIQED
                     VKFLIRYHMMPAALPILPVQKTEALIRDPRFSVLLELFRCDEFSSFKNAERYYDACNM
                     YQSIRRNLRNPYRKADGSKIHASKRSYSHFS"
     gene            complement(13215..13670)
                     /locus_tag="PK105_03750"
     CDS             complement(13215..13670)
                     /locus_tag="PK105_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60854.1"
                     /translation="MMQTDVDAFWANFEQEIGDKIISKTMGQHFSSKKSQGEWGLLVL
                     TASSIRFRPTPGENWFQSLFRMAAPKVPKETASDIVLPLTLITSIELPKRHFFDFLFS
                     PPFTVFALTYRLGENEEHKILLGADPKSELFMKLISTIPVMQGKCKEND"
     gene            13822..14760
                     /locus_tag="PK105_03755"
     CDS             13822..14760
                     /locus_tag="PK105_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU60855.1"
                     /translation="MRRCYFSIMNRMGGILAGLGYSTIFGFSFLMTKEALEVLNPMEL
                     LAMRFIIAALLMSILVISGIIQVDFKGKPIGLLALMCLFQPIAYFTFETYGLANAATN
                     VAGIILGALPAGVAIMEALLLKERLSALQSAGLSISIVGVILVAVFGQGHNTETKPIG
                     IIFLTGSMLSAVFFNIASKKAAESFSPVERTFAMMWSAAICFFIPALFENIKGKGNIS
                     HLFGEWNRIAGVWGGILYLGILSSVGAFFLINYSLSQLKASQSAVFTNLVTVITVLAG
                     VLIRKEQFSWSQGLSVILIILGIAVVNSSFKEKAKS"
     gene            14786..16051
                     /locus_tag="PK105_03760"
     CDS             14786..16051
                     /locus_tag="PK105_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU60856.1"
                     /translation="MQDAAFYDIHCHLLNLSHPAFVSIIESMRHRPREVIYSQIASFD
                     YLASSLLKRGGENLRNLLAVMDNDCADILFLLEDDLSGQFKEPKKTPGFRQAPPLRNG
                     KLYIADQQFSSFVLTPLIMDFLAPSSFSPETYYNRPPQKWIEAQIIDVLFAICTYREK
                     RPSGFLKIYPFLGINTGHYTAEELSAFLEQWLGNYTKSENDIERRFLSYSFDAIADFQ
                     SYSIFSGIKLYPPMGFDPWPEDPAEREKVELLYSFAETKGVPITTHCDDQGYRIISLE
                     ESLKFTAPSRYRPVLQKYPRLILNFAHMGKRHIRTIRGNEQADWRKDIFDLIIEYPNV
                     YSDFSFTAGEPSFYEELASTLNHLKEPEQEKIIQRILFGSDFMVNLFKVRSYRDYLEY
                     FSESVLSVEKKRLFCSDNPRRFLFGDQKA"
     assembly_gap    16080..16179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16250..17002)
                     /locus_tag="PK105_03765"
     CDS             complement(16250..17002)
                     /locus_tag="PK105_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOU60857.1"
                     /translation="MTEPFEETSPFKKHEGSFLDRTKIIAGDDGLARIADSKVAVYGL
                     GGVGAACALDLVRAGVGYLHVVDYDIVEYSNLNRLAFGYNRYVGLAKTEAFSQAAKEI
                     NPAIQIMSVRSFFSAEAASQIIARECDFHADCIDSIMPKVILIASLYRERSAFISSMG
                     TAGRIRPESIKLGKMQEVKGCALARAVRLRLGKMEIPLDFPVVWSDEPPTKPIVQETK
                     RIIQGSMPFVPQTAGHMMASWIIRRILQKDAP"
     gene            complement(17113..17892)
                     /locus_tag="PK105_03770"
     CDS             complement(17113..17892)
                     /locus_tag="PK105_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU60858.1"
                     /translation="MHNIVMYCDAHLHLVGLEQYEPDFALRALPFDWRGAVVSHNPEE
                     FLQSEALRANLPSTIAGFGIHPQEPDMRHADFLSMLCEQRKVDFIGEAGFDFFGDGPL
                     RSRSSEAMHLQLEAFLFQVHLSAYTQLPLVIHIRKATDVIMAYGRELRRLPSIIFHCW
                     PGRLEEAQMILKKGINAYFSFGTPLLRDSRHAIESLVGLPTSRILSETDAPWQPPHGA
                     EYTSLEHITDVVEKMSSVLNMSSEELRHDLSVNFSAAYLHC"
     gene            complement(18011..>19677)
                     /locus_tag="PK105_03775"
     CDS             complement(18011..>19677)
                     /locus_tag="PK105_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU60859.1"
                     /translation="RGRVFSDRWPTIPQLFDITTDRFPDRACFTIYSPDRVSLNYRET
                     REIILRVASYLASIGLGPGSHIAVTGKNSPEWAVAYIAVLYAGAVVVPIDYQLSTQEI
                     MALIDAGDVTAAFIDEEKYDDIKKGRAGMPCFGLARSMETFVYDLEPPQGFAFSPPQV
                     EVQSTAAILFTSGTTGDPKGVMLSHENLVSDCFLAQANLNISEQDIFYALLPIHHSYT
                     MLAVFIESFSVGAETVFGKRMVVKQILSDLKEAKITMFLGVPMLFNKLLSGILKGVRE
                     KGALAYGLIRTLMAISGFIKKTFGVNPGRTFFGSILEKASLSSIRICISGGGPLDPKV
                     FRQYNQLGVDFVQGYGLTETSPIITLNPVEHYKETSVGKVLPETEMIIDNPNEDGIGE
                     IVVRGPMVMQGYYKMPEETAEVLSPDGWLRTGDVGKLDDENYLYLAGRIKNVIVTEGG
                     KNVYPEEIENEFQLFDEIDQILVRGYIPEGKAVAEEIESLIYPNQDWVSQNNLSQEQV
                     ETRIHDIVNQVNQRLRSYEKITRIHILKAPLEMTTTKKLKRHSAKE"
BASE COUNT         5444 a         4214 c         4619 g         5300 t
ORIGIN      
        1 gtgatagacg aagggggagg acatggacct catacagcag attcaagcgg aacaaattcg
       61 aactgacctg tctgatttca gggtcggaga taccgttcgc gtcagtttta agattgtaga
      121 aggcaagtct gagagaattc aagcctatga agggcttgtc atagcgatta agaacgcggg
      181 agtaggtaag acctttactg taagaaaaaa ctcatatggc gtaggtgtgg aacgcgtgtt
      241 tcctttcaat tcacctcgcg tcgaaaaagt ggagataata aaagctggta aagtgcgcag
      301 agctaaactc tactatattc gcagcaaagt gggtaagaag gctaaagtca agactcttgt
      361 cggaggtcgc aagagccaga agcaggaagc ataagaacca tttgatctct agagcgattt
      421 ggaaatcgag ctgcccaggc agaagccttg gcagcttttt tatataatcc taggctttcg
      481 cgaacatttt tgaatcgaga aatctatttt ggcgcgcatt aaatataaga atgcagattt
      541 ggacacgatg cttatgtcta aggctgaagg cgataaatgg aaaagaaaag gaagagaaga
      601 ggagattatg gcaggccgat atctcgagag tattggctgg aatgttcttg cgcgcaattt
      661 tcgtagcagg cgaggggaaa tcgacatcgt agcaacacgc gataatattc tgtcctttat
      721 agaagtcaaa tcggcaaatc aaatcaccga gcgcgatctc gagcatgtca taggagccaa
      781 aaagcgcaat tctataatag aaacctctaa actatttctc gttattaatc gaaaatataa
      841 acagtatcgc atccgttatg atgttatgct gatacaagag agtgcctgtc ttaggcatat
      901 agaaggcgca tttgcggaac acgatggaga ataataaggt ttctagagat attcctgcat
      961 gggctggttc cactccggat gatcttttat attcagacta tgatataacc gaagagatga
     1021 aagccacaaa ttggaagagg ctttatgaga tcaagcataa gctagtggat atcggctatt
     1081 tggatggggc gattgcttcg attgcagaaa tggtatcaaa tacgataagg aataaaccga
     1141 aatgaactcc gagccaaacg caaacaagca accgaacaat ccacccaaac atgaaagacc
     1201 ggggaaaaga attgggagcc agcattttag aaagcatcga tcaacacgcg ccggtgcaca
     1261 aaaaacagct gaccgcaatc aggagaatca tgcgaggatg ggggaaccgt cggtagagag
     1321 ctcaagacaa gaatccaaga tatgtaaagt ctgcggtaag cccatatttg atcttgcagg
     1381 cgcgattgct tcaagagaag atggcgaacc cattcatttt gattgcgcaa ttgaaatcct
     1441 ctccaaggaa gagaatctgg cgccggatga gaagctgatc tatattggga gcggcttttt
     1501 cgcggtttgt gtgcagtctt cttcggacaa actggagatc agacgaaaga tccgctggga
     1561 gatttctggc acccaacacc cctggcgcaa gccgatgatc tttacaccca atttgcctta
     1621 gtttccttag atgctctagg tgcttcttga tgttccattt ccacagatgt cttaaaagat
     1681 atatgcctca agtgcgattg ggacgaataa gatcgaaacc acagtaaggc gctagggctt
     1741 tcgggatcat tacagagcca tccgcttgct ggttattctc aagaatagcg ataatggcgc
     1801 gggagcaggc aattgctgtg ccgttcagca tatgcacgaa gcgatttttg ccatccttat
     1861 ccctatagcg aacattgagt cttcgagcct gataatcggt acaatttgag gtcgatgtta
     1921 tctctcccca ttcgccatta ttcctgcctg gcatccaggc ttccaagtcc cacttgcgat
     1981 aggctggggc accgagatct cctgtgcagg tgtccacgac cctgaatgga atatcgaggc
     2041 tcgaaaaaat ttcctcttcg atcgagcgaa gctcttcatg tattaactcg gagtcctctg
     2101 gtaggcagta tacaaacatt tctacctttg tgaactgatg cactctgtag aggccttttg
     2161 aaaactgacc ggcggcacca gcttcacgcc taaagcaatg agaaacgccc gcatagcgga
     2221 taggaagttg gcttttttca aggacagaat cgctatgata acccccaagc gtgatttccg
     2281 ccgttcctat taggcaagct tcttctcctt cgagagtata aacattcgat tcggggcccc
     2341 gcggaatgaa gcctattccc tccagaatct gtgacttggc aatatccggg gttgtcataa
     2401 gagtaaagcc gtgttttgat agcatatcca gcgcatatcg caccagggcg agctcgagga
     2461 tggttccctc atttttcaga taataaaatt tggtccccga gactttcgtt gctgtatcga
     2521 agtcgagtat atccagactc tggccaagtt ccacatgatc cttaggagga aaattgaact
     2581 gagttggaga accgaccctt ttcacttcga gattatcttt atcttccttg cctcggggag
     2641 ccttaggatg agccatattg ggtattttgg ccaattcttc ggcgaaggct ctctctattt
     2701 cctgaagttc tgcttcgagg cgagcgatta tttctttgag acgtttgcct tcttctatgc
     2761 gaattgcgcg ttcctctttg gatagattcg acttcatggc ttgggcattc tcgtttctga
     2821 ggcgcctgga ttcctcgagt tcgcggagca atgcattctt cttatcgtaa agggagacca
     2881 ataggtccac atcagcatcc atatatcggt cgcgaatatt ctggcgaacc gcttctaagt
     2941 tttctttgat aaaacgtatg tctagcatgg ctagatacta tccggatgag cagcttggcg
     3001 caagcgggag ttgaagtgta ggccataaac aggctaccat tcattcgtga tagaacacat
     3061 agaacatatg gaacaactgc gagggcgtct cgcagaaatt gatggactcg tttcgaattc
     3121 ggatatactt agggataaga ataattatcg caatcttatg aaggagagag caaagatttc
     3181 agagattctt caggaattcg atgaaatgca aagacttaac tcttcgatcc tggagacgga
     3241 agagctgatt cgagaaacaa aggacgagga attgagagag cttgcagagc aagaaattct
     3301 tgaattgaaa aagcagtatg ctcttgccga agaacggctc gagactcttc ttgcaccgcg
     3361 agatcctatg gccgacaaat cggtcataat ggagatacgc gcaggcacag gaggagaaga
     3421 ggcggctctg tttgctgctg acctatatag gatgtatagt cgttatgccg atggtcgcgg
     3481 gtggaatgtc gagataatga gttcaaacga aaccgagctt ggcggaatta aagagataat
     3541 tttctcgatt tctggagatg cagcatacga gaggttacgg ctggaatccg gagtccacag
     3601 agttcagcgt gtgccggtaa ccgaaggttc aggcagaatc cataccagca cggtgacggt
     3661 tgcggtactg cccgagatag aagagacaga aattgagata aggccagaag atctgcgcat
     3721 cgatgtaatg cgttcttctg gtccaggtgg ccaaagcgtc aataccactg actctgctgt
     3781 gcgcattaca catactttaa gcggtattgt tgttcactgc caagatgaga agagccagat
     3841 aaagaataag gcaaaggcca tgagaatcct cagagccagg cttttcgaga tcgaagataa
     3901 gaaacgccaa gcagagcgtt ccgcggaacg acgaaatcaa gtcggaaccg gagaccgctc
     3961 cgagcgcatc agaacctata attttccgca gaatcgcttg accgaccata ggatagattt
     4021 gactctatat aagctcgacc tcatcatgga aggtgatctt gatgaattgc tcgatgcgct
     4081 gatagcatgg gatagagaac aggcactgat ggccgaagaa cgctaattgt catgaattac
     4141 cgagaactta tcttgaatga atctagcagc ttttccgata gcgatagccc ttttctggat
     4201 gcagttttgc tcatggctca tagcctcggt ataactaagg aaaagctttt ctcaatgctc
     4261 tctgagcgtg tagattctgt cccacaatcc ttcttcgata tgacagaacg cagaagaaat
     4321 ggcgagagca tagcccatat tcgcggtttc cgtgagttct atggtaggga atttctagtc
     4381 aacagtgatg tattatcgcc ccggcaagac acagaggtct tggtagaggc tgtgcttgaa
     4441 tcttcaaagc tattcgagcc ctcagcaggc ataaatggga gtcttgatgt agtccgagaa
     4501 atttccattc tcgatctttg taccggttgc ggcgcagtgg caatcagtct cgcagccgaa
     4561 atgccaaatc ataaggtaac agcctcagat atatcttctg cagctctcga ccttgcaaag
     4621 aagaacgcgc aacggctttt gcccgctcag tccttgattt tcatagaatc cgatcttttt
     4681 tccgaaatag atggaaagtt cgaaattatt gccgcaaatc ctccttatgt tccgcattat
     4741 caagcggagc aattgattgc caaaggatgg aaggatcccc ttatctctct ggatggaggt
     4801 atagacggca tggagtatat ccggaaaata atcatggagg gatatgaata tttatcgcat
     4861 aatggcgtat tattattgga gatggatcca caccaggcat cggagtctct ggctctcttc
     4921 ggagagatgg gctatataga tcttaaaaca tggaaggatc tcacaggaag tataagggta
     4981 gtaggcggac gccatggatg aattcgttaa tgaattttct tctaagatcg agcatcgttt
     5041 caatgatgaa gagaagaatc ttcttataaa tgctctggat cacgctcata gtgttcatgg
     5101 agaaagaaaa cgagcatctg gtgagcctgc ttattatcat gatatcaggg ttgctgaaat
     5161 tctcctcgac cttggtatgg atcttgactc aatccttgct ggtcttctcc acgatacaat
     5221 agaggagcga atatcgggtt ttgtccagga tgcgaattta gatagaaata tcgaaggaaa
     5281 acctgcaaac agagaaaaat cggcattgga ggagtcccga atttcgccaa gagggataaa
     5341 acagactacc aaaggcgaac acgcattgaa gaaaaaaagc tcaaacgcat tgagttccct
     5401 tcttgcggcg ccagatcctt ttattgagga acgatttggt ccccaaaccg cggtgatcgt
     5461 tgctggagtc aacaggctgt catcggtcaa ggcaaagaac aaaaccgtgc atgctgctga
     5521 aacaatgcga aaaatgcttt tcgctttgac tagcgatatt cgtgttatcc tcgtaaaact
     5581 cgcagataag ctagatagca tgcgcactct gaaatatcta ccggaagatc gccagaaaga
     5641 gatagctacc gagtgcatag atatctacgc tcctcttgcc gatagactgg gtatttcctg
     5701 gctaaaggat gaattagagg atttgtccct gaaagctcta aaccgtgagg tgttcgacca
     5761 gataaaggaa attgtaaatt cgcgcaagca tgagcgtcag gactttctaa agaaagtgac
     5821 ggataagatt ctctcaacag ctgcaaaaga ggggatagat gtcgaggtca gcgctcgtgc
     5881 taagcatttt tactccatat atcagaagat gcgcaggcgc gggaaaggtg ccgaagagct
     5941 atatgacctt cttggcatac ggattatctg caatggcata aatgattgtt atgctctgct
     6001 tggactcgtg caccggctct ggaagcccat tgatggtcga ttcaaagact atattgccat
     6061 gccaaaagca aatggatacc aaagcctcca tacaacagtg atcgcatatg gggggcaact
     6121 gctagagata cagattcgaa ctagagaaat gcaccttgtg gcggaatatg gagtagcatc
     6181 gcattggctg tacaagaaag gtaccggggc agagattcca aagcttgaag atttgccgat
     6241 tatgaacaag ctaaggcagt ggaaccagtt tattgccgaa ggcgacagct atctagaaga
     6301 aataaagcaa gagttcctgg gtgattctat atttgtcttt accccaaagg gagatataat
     6361 tgagttgcct tctggttcga cacctataga ctttgcattc gctattcact ccgacgttgg
     6421 ggcacactgc cttggcgcaa agatcaacgg tgccatagca cctctcgatt ccgagcttcg
     6481 caatactcag attgtcgaaa tcattacttc acccaatgca agacctaatc tcaattggct
     6541 caagatagcc cgcactagta aagcacgctc aaagatacgc cagcttcttg tacagactgg
     6601 ccaggccgtg gcgatagaca agcacatagt tgcggctcgc aagagcgaaa aagctgctca
     6661 ggaaacggtt caggctcgcg ttcacggagc cgatcgatct caaatcgaag ctcgccgcgg
     6721 cgaatttcct tccgttgtgg agttccatag cctaaagcct ggccttgtgc ttcgcagtga
     6781 gaaagtgggc gtttcggtgg gaggcatgcg caacttgatg attcgcatcg caggctgctg
     6841 taaaccggtt actggcgatc gtatcgtagg ctatgtttcg cgtggtcgtg gaattatcgt
     6901 acatcgcgaa gactgcagat ccatagcttc gattgcggat ttcgaagagc ggcgcatcga
     6961 tgtcaaatgg gaagacgaag cgatcaatac tacggcacgc taccgtgtaa ctaccaaaaa
     7021 gagctacgat atcttctcag agatagaatc caccgtccgc agatgttcag gacggcttaa
     7081 ggaaggcaag ctgggagagc gtggcgatgg gacactctca ggttttttta ttctggaagt
     7141 cgattcacgc gaaggcctaa agcgcgttgc caaggcttta cgcacattac cgagcattct
     7201 ctctatcgaa gaagagccaa tataggcctc ttattcagcg ccaccagaag gcattcttgg
     7261 tgcttacggt ttcgaatcca tagcttgaaa aactcttctt gttgacctca tatagatctg
     7321 gattgaggcg caccatcact acctctagat ttttctgaat gtaagtatcg acaaattggg
     7381 agatattctt ctgtctggtt ttcattataa aatcgctttc ttgaaaaatc agtgatggta
     7441 ttccccagct ccatagtgtg gccatatggc aagcggaact catcgcgtct ccttgaatcg
     7501 ccattatttc ttctaatagc gcttgacaag ctcgatcata atcggccgcg gaaaaatagg
     7561 atggattggc tttaaccata taaagctcgg ttccacgcac ggcttctttg aaagcaaata
     7621 cagtttcggc gggcttcttg ccggctggca caatcgataa gacactgatc caggatgagt
     7681 ctgtgttagg tacatagtcg atcctgatat tctcgggttc cgatccctta ggcatgcttt
     7741 tgcgaactgc catctgaaat cggcttgtag gttcgtttgc aagttgctgc agcatcagcg
     7801 ctgcgaaata ggaagtagtg tcctttggat ccggacccct gtatcgcatc tctatcttca
     7861 tgtgatctgc tgaaatgtca ctgtcaggaa aaacaagaaa tattggcttc gtgacgcccg
     7921 gtctaggaaa tggagcgaga tttgctggcc atggattctt ggctctttgt agcaggccaa
     7981 agcgtgcctc aatcaaggac aaaacctgat tggggtcgaa ggcaccggca attgaaaggc
     8041 aagcattgtt gggaacaagc caggaagagg caatgttctt tagctgtgca tcatccgctt
     8101 tttccaggac attctcggat cccaatatgt cgattctata aggcgcctta gcataaagct
     8161 tcttatttat ggccatcgag cttacaagat cattatcaga ggtttcattc tgaatgagat
     8221 tcaagataga ctgccgagct agctctattc cgtctttttg agcaaagatg aattctcttc
     8281 tttcctgcga aaagagatag agaagagtgt caaatgcctg gaccaagttc tcttttttga
     8341 acgaaatacc gaattcaaat ctatcgatcc ccaatccacc tcttatatcc tctgcttcaa
     8401 gcacatcaat cgcggctgcg ggctccggaa taccaggatt cgccgcggga ccattaaata
     8461 gaacatactc caaaagcttg aatagaccag cagttgaagc tgtctgagaa tttgctcctg
     8521 cgcgataggc aaggattatg gatatatctt cttcatattc gatgggattg agacatactg
     8581 tcattccatt tgcgagtgtc ttgatttgat attgtgcgga agcagaggag ccttttgaga
     8641 gtattaaaaa taggatcact atccatagaa atctgcggct atgttccacc tgcttatagt
     8701 aaaaacagag caacttgttg tcaatcttga ggcgtaattt cgttactatc tatttccgaa
     8761 tcgtcttctg tatggtattc cccttgagag aaatctccaa aaagacgttt gcgagaatct
     8821 tcgagccaat gagaaacctc atcgcgggta aaaaacatat cttgcttttg aggatcgagg
     8881 aagagtttcc atggctcgac ctttagtgca gatgaaatct tcaagattcc tttactggaa
     8941 ggaaaattcc tgccggtctc tatatttgct ataaaaccag gagagaggtt agccagatgg
     9001 gcaagctctt cctgggacat ctgaagaata gcccttctca agcgaagatt agaagcgaag
     9061 caatatcgca cgtaatccat ttaacataca ctacctcaat atgagatatg tctccgcacc
     9121 gagagcatgc aaatcacctt ctatgacgaa ggatcgtgcc aataaatgta attccatcct
     9181 atattattct aagagcgatt gaccgctcca ttggaagtat ttacaattaa tgcatgttca
     9241 tcagagaaat atatataaaa aggaggagag cgttcttaga cgctatccgc gaccgaggaa
     9301 taacaagtgg gaaagttcta ttgctcgcaa atgaaaactg tccgcgcaat tatcccgaaa
     9361 actactaccc ctttagacag gactcttcct ggttatactt gatcggcctg aatatcccta
     9421 gtctcgcttg cactctggat attgagagtg ggggaaactg cctttatgct gatgagccag
     9481 gccttgaaga gatcatctgg actggagtat taccttctcc atcagagctg gcctatagcg
     9541 caggtataga taaagtattg ccaacttcgg ttctttccaa tactgtgggg cgagcgctta
     9601 agcaaagaga gcctgtcttt attcttccgc cttatcgaga tagacaaatt gcaaggtttt
     9661 tggatttatt tgctgtctct gtatcgaacc tagaggagct tatctatcgc catatcgatc
     9721 cgcgcataat tctggctctt gtaagtgtgc gtgaaataaa ggagcctgaa gaactatccg
     9781 aactcgagcg agcggtatct atcaccaaga tcatccatga agagcttatg catgaactaa
     9841 aggctggatg gacagaaaaa gatgcagctg gttttgtgtc atctcgcgcc agcgcgcaag
     9901 gctgtgaact cagctttgcc actatcgcaa cctgtcgcgc ttcggttctg cacaattccc
     9961 ccaccgagca tgtaaccaca gagcatgata catttctgtt agatgctggt gtggagacga
    10021 ctttgggcta tgcgggggat cttacgacga cctttcctgt aggaccgagc tttagccatc
    10081 aatcgagaga aatatatcaa gtgctatatg agaccctaca gacagcaaca aatgcgcttg
    10141 ctcccggggt aaggtttatc gatgtgcaca agattgcttg tctgaccata acaaaaggcc
    10201 tcttatctct aggccttatg aagggggatc cggtcgaggc agttgaatct ggtgctcacg
    10261 cactattctt tcctcatggt ttgggacatc agataggctt ggatgtacat gacatggaag
    10321 gtctaggcga agattatgta ggatatggag atgaactaaa gcgaagcgag cagtttggat
    10381 tgagctcgct caggcttgca aagaccctta agccagggat tgtccactcg gtcgagccgg
    10441 ggatatattt tataccgcag ctcatacaaa aatggcgagc tgagcggatt tgtgagagtt
    10501 ttctaaacta cgatatgatc gaaaaatgga tgccgattgg tgggatgcgc gtcgaggaag
    10561 attggtgcat aaccgacaaa ggagcccgga gattgggtcc ggcattcgac aggagcattg
    10621 aagctataca gaaagtcagg gcaaatagat gaagacagta ttcgtctccg catttctcga
    10681 ggattcaatt gttgtaaaga ctatgctcga atctgctggc attccagctg aagtactctc
    10741 tgatcagatg ctggatataa accccttcta ttcgatagaa ccaaaaggag caaaggtaat
    10801 tgtcccggat agttcggaag aagatgccaa agcgatagtc gcagactata gggaacacaa
    10861 acagtcgaat aagtaattct caatcatttt gcccattatt attaggaaat gttttatgaa
    10921 aagaaagttg tttgttccga tcacgattac gctggcaagt ttgcttttga tcggactatt
    10981 gatctattct attcagaggc atattgcagg tcggaacttc gagtctgtca atgatgcgct
    11041 ctcaagtatc attgagatac aagcgtatgc attctataac ggttgtcttt caaacaacca
    11101 gacctataac ttagttatac aaggggaatt tgccgaggcc aatgcccttg tacaaaaagc
    11161 aaccgattca ttttctctca tagatctggc agaaattgtg gaaggcaaac caccatcgga
    11221 tagttttgat atcattatgg atggagaact gttgcgcata gagtgtccaa tccgcaatga
    11281 tgacgaaaca gctatcgtcc caaatatgat tggcgtggcg attatccaag ctcagagact
    11341 attgaatact gtctcggcta aggatttcag aatcgatcta gctcatggcc gcaagactac
    11401 ttatggaatt ccaatatcgg ctaattttac cgggaatcgc ttatgggatt ccataattat
    11461 agcgctattg acaggctttg tagcgatttt catttttcag catttttata ggaatgcaaa
    11521 cacttttctg gacactcgcg gtctcgaatc tataatctat ctatttgagc agaccgagag
    11581 aatctcagcg agccattcaa ggaatgttgc catgcttgct ctctttgttg gtaagaatct
    11641 tggctttaga ggcaaaagac tcaaggatct ctatattgcc gcattgctcc atgatatcgg
    11701 caaaataggc ataccaaaca acattcttac taaaaacggc aagctcgatc catgcgaatt
    11761 tgtccagatg agacagcatc ctgccatttc cgcacgtatt cttagaagct tcaaggaatt
    11821 tgagcgactt agcccaattg tgctctatca tcacgagaga gaagatggtt cgggatatcc
    11881 ggaaggtttg acaggcaggg atattccatt cgagtctaag atcatcgctg tagtcgatgt
    11941 attcgaagca cttataggag agcgtcctta tcgcgatcct atatgcccgt taagcgcttt
    12001 ccagaagatg agatcgatgc cactcgatca aaaaatagta agtctgctta tggaccacta
    12061 tggagaattg gatggtttta ggccaccgcg ttgggccctg tcttatagtc catggatgct
    12121 cacttcatga gaagtgcgaa tatgagcgct ttgaagcatg aattttggat ccatcggctt
    12181 ttctatatgg atttctaaga ttacgacgaa tagactgata catattgcaa gcatcgtagt
    12241 atcgttcggc attcttgaaa ctcgagaatt catcacagcg aaacagctcc aacaaaacgg
    12301 aaaagcgggg atctcttatc agcgcttctg ttttttgaac aggaagtatt ggtaatgcgg
    12361 ctggcatcat gtgatatctg attaggaatt tgacatcttc ttggatttct tctgagaatc
    12421 ctagacgaga aagaaatttc tccgcacgtt tggctcccag ttcggcatgt ctgtcgaagc
    12481 gatggccttt ttggctttta gattctgatt tgccaatatc atggagaagt agagcaagcg
    12541 agagagtaat atcgcgggtt tttcgatacg aaaatgtctg catagtatgg ttccatccat
    12601 tgccctcggg atggaagtct tttgaatgat cgacattgtc gagaagcgcg atttcaggcc
    12661 attcccgagc aataaagccg ctttgcttga gcacctcgaa tcctgagact ggatagggcc
    12721 cagagagaat gcctattaga agatctcttt gaaaaagtac agatggcgaa ggcggaagag
    12781 cgatggatcc aattgttttc ggaggtaact cggatagggt agctagaagt gtgaaatcga
    12841 acaatgatct ttcaatctca gaatattctg agctggatgg ttggatattt ctggctttta
    12901 gagaattgta tatatcgcct ttatcataat aaacgccagt agaagggctt cttttaaaat
    12961 tcatccaagg gctgcccaga ttttcttcga gcgcatcctc ggtacagata aaccgtactt
    13021 cgtcgcatag agacgcatcg gcgtaaggca gattagggta ttctagaata tcgaatacgc
    13081 gggctagctc taccagcgaa cattgtaccg caaggaactt taatggccga ggtttctcgt
    13141 ccaagtatgc atcaagcgca gtaatgccat attcgaagaa aagcaagtgt gcttcttcga
    13201 gccgtgaata aatctcagtc attttccttg cactttcctt gcatgacagg aattgtcgaa
    13261 atgagcttca taaagagctc tgatttagga tctgctccca ataaaatctt gtgctcttca
    13321 ttctctccca agcgatatgt caaagcaaaa actgtaaaag gagggctaaa gagaaagtca
    13381 aagaaatgtc ttttgggcag ttcaatcgat gttatcaagg ttaagggcaa gactatgtcc
    13441 gatgccgttt ccttagggac cttaggtgcg gccattctaa agagcgattg gaaccaattc
    13501 tcgcccgggg taggcctgaa gcggatagac gaagcggtca atacgagaag gccccattcg
    13561 ccctgtgatt tctttgagct aaaatgttga cccatcgtct tagagataat cttgtctcca
    13621 atttcctgtt cgaaattagc ccagaaggca tcgacatcgg tttgcatcat gattttttcc
    13681 tttatctgac acagtcatag tctactataa atacaatttc atttcatctc tatgcaaacg
    13741 actcatcatg ctatggttta gtcatgaaaa cagctcattt tgcagacgat ctatcgaccg
    13801 gcccacatcc tggaattatc aatgagaaga tgctattttt ctatcatgaa tagaatgggc
    13861 ggtattctgg caggtctagg ttattccaca atattcggct tttcttttct tatgacaaaa
    13921 gaagccctag aagtgctgaa tccgatggaa ttgctcgcta tgcgatttat cattgctgcg
    13981 ctgttgatga gcatccttgt catttcaggg ataatacagg tggattttaa agggaaaccc
    14041 atcgggcttc tggcgctgat gtgcttattc cagcctattg cctacttcac ctttgagacc
    14101 tatggacttg ctaatgcggc gacgaatgtt gcaggcataa ttcttggagc attgcctgca
    14161 ggtgtggcaa taatggaagc tttgctgttg aaagagcgtc tcagcgctct tcaatcagct
    14221 ggtctttcga tatcgatagt tggggttatt cttgttgcgg tatttggaca aggtcacaat
    14281 accgagacaa agcctatcgg gattatattt ttgaccggat ccatgcttag cgcagtcttc
    14341 ttcaatattg ccagtaagaa agcggcagaa agcttttcgc cggtagaacg aacctttgcg
    14401 atgatgtggt ccgcagccat ttgttttttt atacctgcgc tctttgagaa tatcaaggga
    14461 aagggcaata taagccattt gtttggtgaa tggaatagaa tagcgggagt ctggggcgga
    14521 atactttatc taggcatact ctcttctgtg ggagcatttt tcctcataaa ttattctctc
    14581 tctcaactca aagcctcaca atctgcggtg ttcaccaacc ttgtaacggt cataacggtg
    14641 ctggctggag tcttgatccg aaaagaacaa tttagttgga gccaaggatt gagtgtaatt
    14701 ctgataatat tgggcatagc agtcgtgaac tcgtccttca aagagaaagc gaaatcctaa
    14761 ggcgcaattt agctgaggag gcacaatgca agatgctgcg ttctatgaca ttcattgtca
    14821 tcttcttaat ctatctcatc cagcctttgt atcgattatc gaatcaatgc gccataggcc
    14881 tagagaggtt atttattcgc agattgcatc attcgactat ctcgcttctt ccctgctcaa
    14941 aaggggcggt gagaatttaa ggaaccttct ggctgtaatg gataatgatt gcgctgacat
    15001 actctttttg ctagaggatg atctctctgg ccaattcaag gagccaaaga agacaccagg
    15061 ctttaggcaa gcaccgcctt tgcgcaatgg gaagctttat atagcagatc agcagttttc
    15121 ttctttcgtg ttaactcctc ttatcatgga ttttctcgcc ccttcatcct tttctccgga
    15181 aacctactat aatcgtccac cacaaaaatg gatcgaagcc cagatcatag atgtattatt
    15241 tgcgatttgc acataccggg agaaacgacc gtcgggcttt ctgaaaatct atccctttct
    15301 agggataaat acgggacatt atacggcaga ggagctttct gcttttctcg aacagtggtt
    15361 gggcaattat acaaagagcg aaaatgatat cgaaaggcga ttcctttctt attctttcga
    15421 tgcgattgct gattttcaga gttatagtat tttttccgga ataaaactgt atccccctat
    15481 gggctttgat ccatggccag aggatccggc ggagagggaa aaagtcgaat tgctatatag
    15541 ctttgccgaa accaaaggag ttcctattac aactcactgc gatgatcagg gttatcgcat
    15601 catttccttg gaggaatcat tgaaatttac tgctccatcc agatatagac ccgtcctgca
    15661 aaaatatccg aggctaattt tgaattttgc tcatatgggc aagcgccata ttcgaaccat
    15721 acgtggcaac gaacaggcag attggcgcaa ggatatcttc gacttaatta tcgaataccc
    15781 aaatgtatat tcggattttt catttacggc cggagaacct tcattctatg aggagctggc
    15841 ttctacgtta aatcacctaa aagagcctga gcaggaaaaa ataatacagc gaattctatt
    15901 tggttcagat ttcatggtga atcttttcaa ggttcgttcc tatagagatt atctggagta
    15961 tttttctgag agtgttctaa gcgtagagaa aaagcgcctt ttctgttcgg acaacccgcg
    16021 aagatttttg ttcggcgacc aaaaagcata atagatcagc gaccaataag cataataggn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttttgttcgg cgaccaaaaa
    16201 gcataataga tcagcgacca ataagcataa taggcaattt caagcctaat caaggagcat
    16261 ctttctgcag aattcttctt ataatccagg aggccatcat atggcctgct gtttggggaa
    16321 caaaaggcat cgaaccttga atgattcttt tggtttcttg aacgataggt tttgtaggag
    16381 gctcgtcaga ccatactaca ggaaaatcga gaggaatctc cattttgcca agtcgcaggc
    16441 ggactgcgcg ggcaagagcg cagcctttca cctcttgcat tttgccaagc ttgatcgact
    16501 cgggccttat tcggcctgcg gtccccatgc tagatatgaa agctgagcgc tcgcggtata
    16561 aagaggcgat aagaattacc ttaggcatga tagaatctat gcagtctgcg tgaaaatcgc
    16621 actcgcgagc aattatttgt gaagcagctt cagctgagaa aaatgatctt acactcatga
    16681 tttggattgc agggtttatt tctttggctg cctggctgaa tgcctctgtt ttagctaatc
    16741 ctacatatct attatagcca aaggcgagcc tattgagatt cgagtattct acaatgtcat
    16801 aatccacaac atggagatat ccgactccag cgcgaacaag atccaatgcg caagcagctc
    16861 cgacaccgcc aagtccgtag actgcaactt tggaatccgc aattctggca agtccatcat
    16921 caccagctat tatttttgtc ctatcgagaa atgatccttc gtgttttttg aaaggactgg
    16981 tttcttcaaa gggttctgtc atgtatttcg ctccgttctt catatattct atactgctac
    17041 gaaccttgtt tataaggatt gagaatggca ttaggaagcc ttctcatgca atttttatac
    17101 acaaggttcg tatcagcagt gaagatatgc ggccgagaaa tttacagaga gatcatgtct
    17161 caattcctca gagctcatat tgagaacgga ggacatcttt tcgacaacat cggtaatgtg
    17221 ttctaggctt gtatattcgg ctccatgagg cggttgccag ggtgcatctg tctcggaaag
    17281 aattcgtgaa gtaggcaggc ccaccaaaga ctcgatcgcg tgcctcgaat ctctcaataa
    17341 tggtgtgcca aaagaaaaat aagcgtttat tccttttttc aaaatcatct gagcttcttc
    17401 gagtctccct ggccagcaat ggaagatgat cgaaggcaat cgcctaagct cgcgtccgta
    17461 agccattatg acatcagtcg cttttcttat atgaattacg agcggaagtt gggtataagc
    17521 cgacagatga acttgaaaaa gaaaagcctc gagctgcaag tgcatggctt cggagcttct
    17581 tgaccgcaat ggtccatcac cgaagaaatc gaagccagcc tctcctatga aatctacttt
    17641 tctttgctca cataacatgc tgagaaaatc tgcgtgccgc atgtctggtt cttgaggatg
    17701 aattccaaat ccggcaatag tagaaggcaa gttggctcgc aaggcctcgc tttgcagaaa
    17761 ttcttcaggg ttatgagata caaccgcgcc tcgccaatca aatggcagag ctcttagagc
    17821 aaagtcaggt tcatattgct cgaggcctac caagtgtaaa tgcgcatcgc agtacatgac
    17881 aatattgtgc aactataaag catgctggtc aacgtttgta tcatcttcta atagaccgag
    17941 ttcaataacg aacagatcga aatgggtaaa ttcccgagac cgtaaaatcg tagctgccaa
    18001 gtcaagctta ctattctttt gcgctatgtc gtttgagctt ctttgtagta gtcatctcga
    18061 gcggggcttt gagaatatga atccgggtga tcttctcata agagcgcagc ctttggttta
    18121 cttgatttac gatgtcatga atccgcgttt cgacctgctc ttgggaaagg ttattctgcg
    18181 aaacccagtc ttggttggga taaataaggc tttcgatttc ctcagccact gccttccctt
    18241 caggtatata gcctcgaaca aggatttggt ctatttcatc aaagagctgg aactcgtttt
    18301 cgatttcttc aggatagaca ttcttgccac cttcggtgac gatgacattc tttattctgc
    18361 ccgcaaggta tagatagttc tcatcgtcta attttccgac atcgccagtt cgcagccaac
    18421 cgtcaggaga aaggacttca gctgtctcct ctggcatttt gtaatacccc tgcattacca
    18481 tcggacctcg gaccacgatt tcgccgatac catcctcgtt gggattatcg ataatcattt
    18541 ccgtctccgg aagcaccttc ccaaccgatg tttctttgta atgctcgact ggattgagag
    18601 ttattattgg ggaggtctca gtaagaccat agccttgaac gaagtcgacg ccaagctggt
    18661 tgtactgtct gaatactttc ggatcgagag gtccgccacc tgagatacaa atgcgaatcg
    18721 aagataggct agctttttcg agaatggaac caaagaaagt tcttccagga tttaccccga
    18781 aagttttctt gataaagcca gagattgcca ttagagtccg aataaggcca taggctagag
    18841 cacctttttc tctgactcct ttcagaatgc ctgataagag cttattgaat agcattggca
    18901 ctccgaggaa catagtgatc ttggcttcct tgagatccga taggatctgc ttgactacca
    18961 tgcgtttacc aaagacagtc tcagccccga ccgagaaaga ctcgataaag actgcaagca
    19021 tcgtgtacga atggtggatc ggtaaaagcg catagaagat atcctgttcg gaaatgttaa
    19081 ggtttgcttg agccaaaaaa cagtcagaga caaggttttc atggctgagc attactccct
    19141 ttggatcgcc agttgtgcct gaggtaaaaa gtattgctgc agtgctttgt acctccacct
    19201 gaggaggact aaaagcaaaa ccctgtggtg gctccagatc atagacaaag gtttccatgc
    19261 tcctggcaag gccgaaacag ggcattccag cacgaccttt tttaatatca tcgtattttt
    19321 cctcatcgat aaaagctgct gttacatctc cggcatcgat aagtgccata atctcttgtg
    19381 tagatagctg atagtcgatg ggaacaacaa ctgcgcctgc atagagaact gcaatatacg
    19441 ctacagccca ttccggcgag ttcttgcctg taaccgctat atgactgccc ggacccaggc
    19501 caatcgaagc gagatacgag gcaaccctca ggataatctc acgagtttcg cgataattga
    19561 ggcttactct gtctgggcta tatatcgtaa agcatgctct atcggggaag cgatctgttg
    19621 tgatgtcgaa tagttgtgga atagtaggcc atctgtccga aaaaacgcga cctcgat
//
LOCUS       DAOOWY010000029        19024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2904_length_19024_cov_10.577099, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000029 DAOOWY010000000
VERSION     DAOOWY010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 19024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19024
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2904_length_19024_cov_10.577099"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..2799
                     /gene="mgtA"
                     /locus_tag="PK105_03780"
     CDS             145..2799
                     /gene="mgtA"
                     /locus_tag="PK105_03780"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF011702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOU60860.1"
                     /translation="MTKFPLILPSLMKKTETKNGGVSSANENKLISICLAPLQSVFET
                     LESSQHGLSEKEAEKRLSEYGHNEIAQSEKLNFWEDMLDRLKNPLVIQLLVIATISAL
                     IGEMLSAAVVGAMILLSVGLSYVLDSRSNREVESLGKRVQSRTYVLRDGVEIEIKMSE
                     VVPGDIVLLQTGAIIPADVRVIWAKDFFVSESALTGESMPVEKSATLPTSTIKSAIEL
                     PNACFMGTSVTSGIARAIVVSTGINTLFGAISKKLIERREESSFDKGIRSFTWLMIRL
                     MLVMVSIVFLIVGMTKGKWLEALLFALSVAVGLTPEMLPMIVTVNLAKGALAMAKKKV
                     IVKRLPSIQNLGAINILCTDKTGTLTQDRVVLEHNMDIIGNKSDEVLNYAYLNSYFQT
                     GLKNLLDRAVLEHVDLNVDQCRLVDELPFDFQRRRMSVVVEYEGDNVLICKGAVEEIY
                     SCCSYYQIDEEIYPLIDMIRADLFEDVEKLNTEGFRVLGIAYREFPRQKTTFTVEDES
                     QLILLGYIAFIDPPKESATKAIELLSKTGVEVKVLTGDNGLVTKKVCRDVGIPFTASI
                     TGPELAALEKDEFSDIVRKCEVFVKLTPNQKEEIVRELRQQGNVVGYLGDGINDATAL
                     KAADVGISVDSAVDVAKEAADIVLLEKSLLVLEEGIMEGRRIFSNIIKYIRMGASSNF
                     GNMFSMLGASFLLPFLPMKPLQILANNLLYDISQTGIPMDNVDPELVAKPVRWDIDNI
                     KRFMMCIGPISSIFDYATFALMWFFFNAKDYLNPALSLAQRDAAASLFQTGWFVESLL
                     TQTLIVHIIRTKRIPFLQSRASAPMILASLLVMGIGIWLPYSPIAGILGLVPLPYIYW
                     LWIAAFLLTYSIITHKVKCWFLKHYEGA"
     gene            2802..3596
                     /locus_tag="PK105_03785"
     CDS             2802..3596
                     /locus_tag="PK105_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU60861.1"
                     /translation="MDSILDALQEGRLFELPENDKDHALQFLAHIIEAFPQIPTGTDI
                     VGNVMAREKSMNTALGRRWACPHARVEFEEDLMCVVGWSPTGIDYGAPDGRPVSIIVM
                     YLVPSNQRNHYLREISTLAKVLKSSPEIDKLSTIRELNDVRNYLLDLISVSKDTVGPD
                     ARARMIRLQAKAAMATQPVAELSNLIIEPVSIIAGMGLKPLALTQNLELMNFIETTAG
                     LTERIETEGKYQNGLWRIIRRSATAYQGGRTVFDCIALMPASTGQR"
     gene            3714..5810
                     /gene="fusA"
                     /locus_tag="PK105_03790"
     CDS             3714..5810
                     /gene="fusA"
                     /locus_tag="PK105_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU60862.1"
                     /translation="MSYTTEQIRNIAIIGHGSTGKTTLLEHILFQGGMISRPETVDSG
                     KTVSDYSEDEIARKISVRSSLTHVNTDGYKINFIDAPGSGDFVGEAILAIRSTEAALL
                     VIDGKSGVQIETVKLWRILERHKRPRMMFITRLDEDRADFSNALADIKEKFRVTPVPV
                     TIPMGEGFSFKGIIDVLNNKAYMRPVSHDQKEVPSEVPPEYSEAVESARALLSEAAAE
                     GNDDLMEKYLLEGELTQEETLLGLKEALAEGKIIPAFAGAGFVNSGTAALMDFIFQSA
                     PSPFARAPEKAKDQDGNDVEISIDPLKPASAYVFKTQIDQFSGRLSYIKAITGTILSD
                     IEMVVSRDLHRERIGKLYTMQGKKLEEIPSLPAGDIGVLAKISSLKTNDTISQFDKPI
                     SYEPLQLPSPVHMLAISAVNKKEEDKLNELLNRAAEEDLTFRVSYNPETRETVIAGMG
                     EQQINMILDKIKSQSKIAAETRIPRVAYRETITKKATAEYTHKKQTGGHGQYARVVFE
                     IEPLERGKDYEFENRIFGGAVSKGFMPGIEKGIHQAMEAGVLAGYPVVDTKTAIIDGK
                     EHTVDSSEMAFKLAARGAFREAMKLASPVLLEPIMNLSVFVEDKYLGDVMSDLSGRRG
                     KISGQNPIGGGIVQIDAQVPQAELLRYAIDLRSMTSGTGSFEVEFSHYAPISGKIAED
                     VIKAAQAFKIQEAEEE"
     gene            complement(5835..6302)
                     /gene="nrdR"
                     /locus_tag="PK105_03795"
     CDS             complement(5835..6302)
                     /gene="nrdR"
                     /locus_tag="PK105_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOU60863.1"
                     /translation="MKCPYCGSPEDKVIDSRTLANGEAIRRRRECISCGLRFTSYERI
                     EEKPLLVIKRDGRREPFERQKIERGLVRALEKRPISQMSIENLVNEIEDNAAEHAKAS
                     NEISSEELGRMVLQKLYSLDKVAYIRFASVYRKYDTLDEFIREIERLENGQTG"
     gene            complement(6299..7213)
                     /locus_tag="PK105_03800"
     CDS             complement(6299..7213)
                     /locus_tag="PK105_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOU60864.1"
                     /translation="MLWIYTIFAVLSAWWVVFIIIAAIRKQKPVFLFLLILFLVWGIS
                     EIILMMSSPPIAAILFIVPFLILIQMMRVVQEYQRGVLFRFGRLQKVMEPGFNLILPF
                     GIDSLRKVDMRTFTIDVPKQEVITRDNVPVIVDAVVYFNVFDSMLAVVKIADYTKGTS
                     LLAQTILRSVLGQHELDEMLTQRAQLGQVLQGLLDDATNPWGIRVSAVEIKAIELAET
                     MKRAMARQAEAERERRAKIIAAEGELQASEKLAQAADVLAKSPASLQLRYLQTLSEIA
                     VEKNSTIVFPLPMDLLNVISKVAEKSSE"
     gene            7422..7772
                     /locus_tag="PK105_03805"
     CDS             7422..7772
                     /locus_tag="PK105_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60865.1"
                     /translation="MDFSEKLKKFMENSAEASREFLEKAADQAQVWGEMGRLKIEIIQ
                     LRTKAQSLTAKLGTEVYNLLVEKNEPMIGSSTPEIEPIIRELQNLDRLIDEKENLYRS
                     KGGKDSDLNIKSKE"
     gene            7811..8482
                     /locus_tag="PK105_03810"
     CDS             7811..8482
                     /locus_tag="PK105_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOU60866.1"
                     /translation="MQKLQPELIIFDLDGTLVDTIVDIASSVNYVLSQFGLPNHEISD
                     YKQMVGNGFRVLVQRVLSPEKSNDDIFFDKILRLSMQRYEEHALDRTRPFPGTVETLE
                     SLYRNEIKLAVLSNKPDHLSKKIIESLFASVKFVAVWGDNKDRARKPDPSATLELCRN
                     ARVDPQKSLFVGDSAVDIQTAKAARIAAVGAVYGYRSREELELAGADYLITSPPELLS
                     IVEFK"
     gene            8623..10371
                     /locus_tag="PK105_03815"
     CDS             8623..10371
                     /locus_tag="PK105_03815"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOU60867.1"
                     /translation="MNHEEIILRAQEYIANETDPSFSEEIKELLQKGDWKELEDRFYR
                     DLEFGTGGLRGIIGGGFNRMNTLVVTRATQGLCDYIKEQFPQKQLSACIAYDSRRKSK
                     EFSLATALVFAANDIKAYIFPELKPTPVLSYAIRKLGADTGVVITASHNPPHYNGYKA
                     YWNDGSQVVPPHDSGIIEKVLKAKSAKQMSKTEARSKGLLVEISQEIDDDYVAMVKSH
                     LLRSYLFSEMGKSVNIVYSPLHGTGARLLERIMKELGLNVLTVPEQREPDGEFPTVSY
                     PNPEESAALAMAIELGKKTHADVVMATDPDADRLGIAVPDKAGEFVLVTGNQLGSLHL
                     DYIALTLKEINSMPPRPAAIRTIVTTELQKAIAEKYGIESFECLTGFKWIADLMRRFE
                     TENIDFIYATEESYGHLIEKEVRDKDGISAAALTAEMTLYWRSQGKSLLDRLEDLYKE
                     HGYYEEKGLSFYFEGEQGMRIMNSIMEDYRKQQPDQFGELAVICTRDVKAGTEWDRDG
                     RIRKIDLPQSDVIQWRLEDGTLLTVRPSGTEPKIKYYILCHDQSSELSLSKEITQKKI
                     ALIAEAITAMVDSHRK"
     gene            10599..11516
                     /locus_tag="PK105_03820"
     CDS             10599..11516
                     /locus_tag="PK105_03820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU60868.1"
                     /translation="MENDFYHNLSMLNSSNFSLLSMKQIEQLASSLCIQGMTMDLDNR
                     AMASLFLPSSGHVLAMLIDDQDSEEYQNLDGISLPARLSYNRIAALHPEMNALRPRYE
                     AFIDVFYRRLLESGISDFPAKWFEQNAQTWNGNVTLPGLSSLPRLDELDYSHTFALDI
                     FLQDVEYLSWNIQRGPVIHSLSNGIVVAAESGWLGFPRKSAALSYIKGGISPKSGNGV
                     IVYSPEEHRYYLYFHLYDLFVKKGDILLKGQAIGHGGNSGINARKKGGGDHLHLEIFD
                     SKSGRFLRNTQIIALLKDSSKNKTAVSPK"
     gene            11538..12632
                     /locus_tag="PK105_03825"
     CDS             11538..12632
                     /locus_tag="PK105_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HOU60869.1"
                     /translation="MNKLFLVGDVGGTNTNLALARHDESGIELIEKAQFSTQNEKSLL
                     DPIKRFLESRRVGGANHKIDFCCISGAGPVQANNSIQLTNAPWGIKASELYEFLRVPV
                     KLINDFTALSYAVTILDHKDKDKVTQLPHPDGSIYEPQDGIMLIIGAGTGLGVGFVDR
                     RGDSVMVFPSEGGHSELSCFDRLSDSFYAWLRKKYDYVPGAELAISGQGIANIFEFIC
                     TEDLAMSIVEGDYEISQKALEKGLSDTARNILDLPRFEWPALISQNARSDAFCNLTME
                     LFVRLYALKAANLVSIFLPAGGVWLAGGISSKNEDWLIEKSRFMRWFERNYATHIRDI
                     LCRTPVLIVKNYDISLMGAAIAALQFSTHH"
     gene            12644..14257
                     /locus_tag="PK105_03830"
     CDS             12644..14257
                     /locus_tag="PK105_03830"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOU60870.1"
                     /translation="MKEQDLHIELLADMKKKNIDIALTLDILNRYNAGQFDHYEKVIP
                     SGVPPLDGIHVIDIRNRSPNNILFKYPKQESILNLEHNDIFLHSAFPAGNKNIFEIRE
                     MGREMLCFTENALEEIGKHLLPKTAYGVLNGGSATSYIDIKKNGSIDESLFEAVRLTF
                     EDLAKDIENKPKGLTPAYINPDGSAGASFLELKMRSRLLIAKELGHGRLSRKPGALAS
                     SAYQKQPDFLPLFQMTSVGNHEELLRAYEEMRVSPYLSDLAHVVGLEPCEWVSGIQPL
                     IAAYTHSAEGLPKRVFDRAYGKHNSSLAIPGGHGQSFMILADIYRTLLKQGMRYAMLS
                     NVDNTGAYLDPIELAILAISGKPAGFDFAFRTPVDVKGGILILAPNGSRNVVDIGPAI
                     GLEEVECLEKSGSAILFNCATGIFDLEWLVPHIEEISRALPIRFSDQSKDAGNYSQAE
                     QVTWEVVSLLPDFIAFAVQKDRRFLAAKMLIEMLLASGFGIDKPSIDPSLKQLGMTMH
                     NGQSWLLENVYGLTKVNGRWLPQESALCI"
     gene            complement(14214..16412)
                     /locus_tag="PK105_03835"
     CDS             complement(14214..16412)
                     /locus_tag="PK105_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU60871.1"
                     /translation="MALLPFFCGKDCGGDACPLLAEVEDGHIRGMHLNPLAGRYIKPC
                     GKGFRAHKNHASPRRLKTPLLRTGRRGSGQFREIGWDEALSYIVQRLIDIRAKDGPGS
                     VLCIASSGSTGALHNSETLARRFFNSIGGCVNVKGNYSCNAATYALSRLLGASRMNSG
                     FDAETIRYSNLIVLWGSNPFEARLGAELPAYLIEAKKRGARIIAIDPRRSISARILNA
                     EWIGIIPGTDAVLGYALLFEMLQDARFDAEFISKYSIGFESLTDYVLGKIDGIPKTPL
                     WASSICGVDANVIIRLASFFWDEKPAMIIPGYSIQRVAYGEEAFRLTMAIQLATRNSG
                     LLGASTGSINNCLPGIKVGTLKELPSSLTKGAFIKEGFTKEAHLWEDNHKSQGIEAEL
                     FTKTVPILRWADAVLSPSSYGLGSIKALYSAGGNFLNQGANVTKNIKAFESVEFAVCH
                     ELFLTPSARYCDIVLPVSDGFEKEDIGIPWAGNYVLYKPEICRALAGTHSDYWIFSQL
                     SERFGLGSVFTEGRTESQWIEHFLAESEIADIEAFRIEGIFLKKGLLRGGLDDFIADP
                     VHCPIGTNSGKIEFASPLWNQNMTLHWQGLMKKGSTEESFREFRLLTPKVSNFIHSQR
                     IGQEDRSECAQIHINPKDMSYIGAQDGEILKVWNKNGTLMASALADDWVQPGTVWIEE
                     GIWTMPIDEVDLCGNPNMLTSDEGTLESISNIMHGIPICIGRIPEATIGH"
     gene            complement(16427..18775)
                     /gene="lon"
                     /locus_tag="PK105_03840"
     CDS             complement(16427..18775)
                     /gene="lon"
                     /locus_tag="PK105_03840"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOU60872.1"
                     /translation="MSASELIPIDQILPGKLPVITLIGKPIFPGIFTPIMVGKEPDIQ
                     LVEQAISADGMIGLVLQKDDSDEPSSDNLFHVGTAAKIIKKINLPDGGINIFISTFKR
                     FKIKKFISKEPPIITAVSYLDDENYDNDEVKALTRALISEMKQLSENNPLFSEEMRLN
                     MINIDNPGKIADFITSILNIEKKDQQDILETLDVRERMEKVLIHINREKELFRIQKKV
                     QAEINEKIEKSQREYFLREELKAIKQELGMAPDARSSDYQRFREKIDSFQFEGEIKEI
                     VEQELEKFNLMEPSSSEYMVTRNWLDLVCSLPWNTSITADFDIKHAKKVLEEDHYGLQ
                     DVKDRIIEYLAVRKLKKDTRGTILCLVGPPGVGKTSIGRSVARALGKQFFRFSVGGMR
                     DEAEIKGHRRTYVGALPGKIIQGLKIAKSRDPVFMIDEIDKMGISYQGDPSSALLEVL
                     DPEQNFSFRDHYLDLPFDISNIFFIVTANTLDTIPRPLLDRMEVIQLPGYVDVEKIEI
                     ARHFLIPRSLERNGLGKNQVKYTRGALLAIIDGYAREAGVRNLEKSLDKIHRKIAKSI
                     VIENRQEEEFKIDKHAVEKYLGKPIFRDEELKRASKPGMAIGLAWTSLGGDTLLIEAV
                     ANPGKEGFKLTGQMGPVMQESAGIAYTYVRNIAAQKYGIQAEFFESRQIHLHIPEGAT
                     PKDGPSAGITMATALLSLVINKRIRDRLAMTGELSLTGQVLPVGGLREKTVAAKRNKI
                     REIIIPAANEKDLDEIPEHVKKGLVFHPVSRMEEVIEIVFGS"
BASE COUNT         5351 a         4284 c         4213 g         5176 t
ORIGIN      
        1 agtcactgca tctttaccga gcccgaagat ccattcccgc gaccatctca cctgacagct
       61 ggtatcgaaa aagtatagaa ccacatattg ttctatgcat tcacctcctt ctgtcaggcg
      121 agcgacagaa atgtgaggtg aactatgacc aaattcccac tgattttacc ttctttaatg
      181 aaaaagactg agacgaagaa tgggggtgtt tctagcgcaa atgagaacaa gctgatttct
      241 atctgcttgg ctccccttca gagcgtattt gaaacgctcg aatcgagtca gcacggtctc
      301 tccgaaaagg aagccgaaaa aagactctca gagtatgggc acaatgaaat tgcacagtcg
      361 gaaaagctca atttctggga agacatgctc gatcgtctta aaaaccctct tgtcatccag
      421 cttctcgtca ttgcaacgat ttctgctctc attggcgaaa tgctatccgc agctgttgtt
      481 ggagcaatga tcctattgag tgtagggctc tcttatgtcc tggacagcag atccaataga
      541 gaagtcgagt ctctaggcaa gcgtgtgcag tctcgcactt atgtgctcag agatggggtt
      601 gaaatcgaaa tcaaaatgtc agaagtcgta cctggggata tcgtgcttct ccagactggt
      661 gcaataatac ccgccgatgt gagagtaatc tgggcaaaag acttctttgt aagtgaatcc
      721 gctttgaccg gagaatcaat gccggtggaa aaatctgcca ctctgccgac aagcacgata
      781 aagtccgcga tcgaattgcc taatgcctgc tttatgggca caagcgttac gagcggaata
      841 gctcgagcca tagtcgtttc cacaggtatc aacacgcttt ttggtgctat ctcgaagaag
      901 ctaatagagc gccgcgaaga aagcagtttc gacaagggga tacgctcctt cacatggctt
      961 atgattcgcc tgatgctcgt tatggtgagc attgtattcc tgatcgttgg aatgaccaag
     1021 gggaaatggc tcgaagcgct actcttcgcc ttatctgtgg ctgtggggct tactcctgag
     1081 atgcttccca tgatcgttac agtgaacctt gccaaaggag ctctggcaat ggcgaagaag
     1141 aaggtcatag taaagagact gccatctatc cagaatcttg gagctatcaa catcctttgc
     1201 accgacaaga caggtactct gacacaggat cgcgtagtac ttgagcataa tatggatatc
     1261 atagggaaca agagcgatga agtcctcaac tacgcctacc taaatagtta ctttcaaaca
     1321 ggtctcaaga atctcctgga tagagccgtc ttagaacatg tcgatctcaa tgtcgaccaa
     1381 tgcagacttg tggatgagct cccattcgat ttccagcgcc gccggatgtc ggttgtcgta
     1441 gaatacgaag gagataacgt cctcatatgc aaaggagcgg ttgaagagat atatagctgt
     1501 tgttcctatt accagatcga cgaggaaata tatccgctga tagatatgat ccgtgcagac
     1561 ctcttcgaag atgtagagaa actcaatact gagggattca gggttctagg catagcttac
     1621 cgtgagtttc cgcgccaaaa gactacattt actgtcgagg atgaaagcca gcttatactg
     1681 ttgggttata ttgcgtttat cgatcctccc aaggagtcgg ctaccaaggc catcgaacta
     1741 ctttccaaga ctggcgtaga agtgaaggtt ctaacgggcg acaacggcct cgtgaccaaa
     1801 aaagtatgca gagatgttgg aatccccttt actgcatcca tcactgggcc tgaacttgct
     1861 gcgctcgaaa aagatgaatt ctctgatata gtaaggaaat gtgaggtttt tgttaagctc
     1921 actccaaatc aaaaagaaga aatagtgagg gaattgcgac aacagggaaa tgtggtcgga
     1981 tatttgggag acggaataaa cgatgcaact gcccttaaag cagctgatgt gggaatatcg
     2041 gtggactcgg cggtagatgt tgccaaggaa gcagcagata tcgtattact tgaaaagagc
     2101 cttctggtgc tggaagaagg tatcatggaa ggccgacgta tcttctccaa cataatcaag
     2161 tacatcagga tgggagcaag ctcaaatttt ggcaacatgt tcagcatgct gggggcaagc
     2221 tttcttctgc ccttcctccc catgaagcct cttcaaatac tcgccaataa tctgctctat
     2281 gacatatcgc agacaggcat ccctatggat aatgtggatc cggaattggt ggcaaaacca
     2341 gtgagatggg atatcgacaa tataaagcga tttatgatgt gtattggacc aattagctca
     2401 attttcgatt atgctacttt cgcacttatg tggtttttct tcaatgcaaa agactacctt
     2461 aaccctgccc tttctttagc acaacgggat gctgcagcca gtctttttca gaccggttgg
     2521 tttgtcgagt cactactaac ccagactctc atcgttcaca taattcgcac taaacgtatt
     2581 cctttccttc agagcagggc atcggctcca atgattcttg catcattgct tgtcatggga
     2641 atcggaatct ggctacccta ctctcccata gcaggtattc ttggccttgt gcctcttcct
     2701 tatatctatt ggctttggat agcggcattt cttttgacct attctattat cacgcacaaa
     2761 gtaaaatgct ggtttttaaa acattatgaa ggagcataac catggactcg atactagatg
     2821 ccctccagga aggcagactt tttgagcttc ctgaaaatga taaggatcat gctctccagt
     2881 ttctagcgca tataatcgaa gccttccctc aaattcccac cggaacagat attgtgggta
     2941 atgttatggc aagagaaaaa tctatgaata cggctcttgg cagaaggtgg gcttgtcctc
     3001 atgcgcgcgt ggaattcgaa gaagatttga tgtgcgtagt gggatggagt ccgacaggta
     3061 tcgattatgg agctccggat ggtagacctg tctcgataat agtcatgtat ctggttcctt
     3121 ctaatcagcg aaaccactat cttcgagaga tatcaacttt ggccaaggtg ctcaagtctt
     3181 cgccggaaat agacaaatta tcaacaataa gagaattgaa tgatgttcgc aattatttgc
     3241 tggatctaat atcagtgagc aaggatacgg ttggaccaga tgcaagagcg cgcatgattc
     3301 gtctacaagc aaaggcagct atggcaactc aacctgtagc agagctgtcc aatctgataa
     3361 tcgaacctgt ctcgataatt gcaggtatgg gcttaaagcc acttgctcta acacaaaacc
     3421 tcgaactaat gaatttcata gagacaaccg ctggcttgac cgaaaggatt gaaactgagg
     3481 gcaaatatca gaatgggctt tggagaatta tcaggcgaag tgctactgct tatcagggag
     3541 gacggactgt attcgactgc attgcgctca tgcctgcaag caccggacaa agatgaatcc
     3601 caactatatt tgtcagactg ctgttgacac cgacgccttt tgaattataa tagctcgtct
     3661 tccttaaatt atgagaggaa attctaaacc atcccaccct gtgaggagtc attatgtcct
     3721 ataccacgga acagattcgt aatattgcta tcattggaca tggtagcacg ggaaaaacga
     3781 ctctgcttga gcacattctc tttcagggcg gaatgatatc tagacctgaa acggtagatt
     3841 cgggcaaaac cgtcagcgat tatagcgaag acgagattgc acgcaagata tcagttcgct
     3901 ccagccttac gcatgttaac accgatggat acaagatcaa cttcatagat gcaccaggct
     3961 ccggtgattt tgttggagaa gcaattctag ctattcgttc gaccgaggct gctctgctcg
     4021 ttatcgatgg caaatccggt gtacaaattg agacggtaaa actctggaga attctggagc
     4081 gccacaagag accaagaatg atgtttatca cgcggctaga tgaagataga gccgacttct
     4141 cgaatgctct tgctgatatc aaagaaaaat tccgagtcac tcctgttccg gtgacaattc
     4201 ctatgggaga agggttctct ttcaagggaa taatcgatgt actcaataac aaggcatata
     4261 tgcgacctgt ctctcatgat caaaaggaag tacccagtga agttccgcct gagtattccg
     4321 aagctgtgga atccgcccgc gcacttctct ctgaggcagc tgccgaagga aacgatgatc
     4381 ttatggaaaa ataccttcta gaaggcgagc ttactcaaga agagactctg cttggtctta
     4441 aggaagctct tgccgaaggc aagattattc cagcctttgc cggagcaggg tttgtaaata
     4501 gcgggaccgc cgcgcttatg gattttattt ttcaaagtgc tccttcccca tttgctcgtg
     4561 ctccggaaaa ggctaaggat caagatggca atgatgtgga aatatctatc gatcctttga
     4621 agcctgcatc ggcctacgta ttcaaaactc aaatcgatca attctctggt cgactttctt
     4681 atattaaagc tataactgga accatccttt ccgatataga aatggtagtc agccgtgatt
     4741 tacacagaga gcgcatagga aaactctata ctatgcaagg aaaaaagctc gaagaaatac
     4801 cttctttacc agcaggagat atcggagtcc ttgctaagat ttcctcactc aagacaaatg
     4861 atacaatcag ccaattcgat aagccaatct cctatgaacc gctgcagttg ccctctcctg
     4921 tccatatgtt ggcaatttcc gcagtgaata aaaaggaaga agataaactc aacgaattgc
     4981 taaatagagc cgcggaagag gacctcactt ttagagtgag ctataatccg gagacaagag
     5041 aaacagtaat cgcagggatg ggcgaacagc agatcaacat gatactggac aagatcaaat
     5101 ctcaatcaaa gatagcggca gaaaccagga ttcctcgcgt ggcttataga gagaccataa
     5161 ccaagaaggc aactgcggaa tatactcaca agaaacagac aggagggcat ggccaatatg
     5221 cccgagttgt attcgaaatc gaaccactgg aacgcggaaa agactatgaa ttcgagaata
     5281 gaatttttgg tggcgcggta tctaaaggtt tcatgccggg catagaaaag ggcattcatc
     5341 aagcaatgga agccggcgtg ttggctggct atcctgtagt cgatactaaa acagcgatca
     5401 tcgatggaaa ggagcacaca gtcgattcat ctgaaatggc attcaaacta gccgctagag
     5461 gagcctttcg tgaagccatg aagctagcaa gcccggtctt acttgagcca atcatgaatc
     5521 tctctgtctt tgtagaggat aaataccttg gcgatgtcat gtccgatctt tcggggcgcc
     5581 gcgggaaaat atccggccag aaccccatag gcggtggcat tgttcagata gatgcacaag
     5641 ttccacaggc agagcttttg cgctatgcga tcgatctgcg ttccatgacc tccggtactg
     5701 gttccttcga agtagaattc tcgcattatg cgcctatctc tggaaagatt gcagaagatg
     5761 tcataaaggc agcgcaagcc ttcaagattc aggaagctga agaagagtga taactagcaa
     5821 taatacgatc tccgctagcc ggtctgtccg ttttccaggc gttcgatctc gcgaatgaat
     5881 tcatcgaggg tgtcatattt tctatacacc gaggcaaaac gaatataggc aactttgtcc
     5941 agggagtata gcttttgcaa aaccatccga cccaattctt cgctcgatat ttcattagaa
     6001 gcttttgcat gttcggcagc attgtcttct atttcattga cgagattctc tatggacatc
     6061 tgtgagatag gccttttttc cagggcacgg acaagacctc gttcgatctt ctgtctttcg
     6121 aagggctcac gcctgccatc tcttttaata acaaggagag gcttttcttc tatgcgttca
     6181 taactggtaa aacgaagccc acaggaaatg cattctctgc gcctccttat ggcttctccg
     6241 ttagccagtg ttctagagtc gatcaccttg tcttcggggc ttccgcaata tgggcacttc
     6301 attctgaact cttttcggcg accttggaga taacattcaa tagatccatg ggcagcggaa
     6361 atactatagt tgagtttttt tcaaccgcaa tttccgatag agtctgaagg tatcgcagct
     6421 gcaagcttgc cggagatttc gctaacacat cggcagcttg ggcgagcttt tcggaagcct
     6481 gaagctctcc ttctgccgcg atgatctttg ctcttctctc tcgctctgct tccgcttgcc
     6541 tagccatggc tcgtttcata gtctctgcaa gctcgatcgc ttttatctcg actgccgaga
     6601 ctctaatacc ccaaggatta gtagcatcat ccaagagtcc ttgaagaact tgtcccagtt
     6661 gagctctttg agtgagcatt tcatcgagct catgctgacc aagaaccgat cggagaatcg
     6721 tctgggcaag gagactggtg cccttggtat aatctgctat ttttacaacg gcaagcatgg
     6781 aatcgaacac attgaaatac acaacagcat ccacaatgac aggcacattg tctcgggtta
     6841 taacttcctg ctttggaacg tctatggtga acgtgcgcat atcaaccttg cgaagtgaat
     6901 cgataccaaa tggtagaatg aggttgaagc ctggttccat cactttttgt aagcgaccaa
     6961 atcgaaataa aacccctcgc tggtattcct gaacgacgcg catcatctga atgagaatta
     7021 agaaaggcac aatgaacaaa atagctgcta tcggaggaga actcatcatc aatataatct
     7081 ccgaaatccc ccataccaag aatagaatta gaagaaatag aaagacaggt ttttgttttc
     7141 gaattgcagc gattataata aaaactaccc accatgcgga aaggaccgca aaaatcgtat
     7201 aaatccacaa cacctcatat accccctatt taaattaagt atagtgtatt tagtactatt
     7261 tcgcaaaagc taaacaattc tctggccaaa tgaatctact gcgctatcga catgcccagt
     7321 tcttttctgt tcgtacgttc cattatattg ctaatttttt tctctgggat tattgtatcg
     7381 atgatgaatt gcctatcata ctgtaaaaaa aggaggtcaa aatggacttt tccgaaaagc
     7441 taaaaaaatt catggaaaac agcgccgaag catctagaga attcttggaa aaagccgcgg
     7501 atcaagccca agtctgggga gagatgggta ggctgaaaat cgaaattata cagttaagga
     7561 caaaggcaca atccctcacc gcgaagctcg gcacagaagt ctataacctt ctggtagaga
     7621 aaaatgaacc catgataggc tcttcgacgc ccgaaattga gcctataata agagagctcc
     7681 agaacttaga tcggctcatc gatgagaagg aaaatctata tagatccaaa ggcggtaaag
     7741 atagcgacct caatataaaa tcaaaagaat aagcgaaaaa ccataatcta tagaacttgg
     7801 agtcctcata gtgcaaaaat tgcagcctga acttataata ttcgatctcg atgggacact
     7861 tgtcgatacc attgtcgata ttgcctcatc cgtaaattat gtgttatctc agtttggcct
     7921 tcctaatcac gagatttcag attacaaaca aatggtaggc aatggctttc gtgttcttgt
     7981 tcaaagagtc ctttcgccag aaaaatccaa tgatgatata ttcttcgaca agatactgag
     8041 actttctatg cagagatatg aagaacatgc gcttgatagg acaaggcctt tccctgggac
     8101 ggtagagact cttgaatccc tctacaggaa tgaaataaaa ctcgctgtac tctcgaacaa
     8161 gccagaccat ctctcaaaaa agatcatcga atctcttttt gcctctgtca aatttgtcgc
     8221 tgtctgggga gacaataaag accgagctcg taaaccagat ccaagcgcta cacttgaact
     8281 ctgtcgcaat gcaagggttg accctcagaa aagcctgttt gtgggcgact cggctgtcga
     8341 tatacagaca gcaaaagcag cacgaatagc agctgtgggt gccgtatatg gttatcgaag
     8401 cagagaagaa ctcgagcttg ccggagcaga ctatcttata acctcgccgc cagaactact
     8461 ctccattgtc gagttcaaat agctgagaac ccaaagatcg atctctcttt tgggaggctg
     8521 acttttctta ttagagcaca atagctataa ttagaaataa aataagaacg cttggaattg
     8581 ctctttatgc gttgcaggat ttggtcttat cgaggagtca ttatgaatca tgaggaaata
     8641 atactgagag cgcaggaata cattgctaat gaaaccgatc cgtctttttc tgaggagata
     8701 aaagagctcc ttcagaaagg cgattggaag gaacttgaag accgcttcta tagagatttg
     8761 gaatttggta caggagggct tcgcggcatt attggcggtg gcttcaatag aatgaataca
     8821 ctagtggtaa cacgagcaac gcagggacta tgcgattata ttaaggaaca attccctcag
     8881 aagcagcttt ctgcttgcat tgcctatgat tctcgtagaa aatccaaaga gttttcgttg
     8941 gcaacggcct tagttttcgc agccaatgat atcaaggcat atatattccc ggagctcaag
     9001 cctactcccg ttctatctta tgctatccga aagcttgggg cagacacagg tgtagtaata
     9061 acagccagcc ataatcctcc ccattataac ggctataaag cctattggaa tgatggctct
     9121 caggtcgtgc ctccccatga tagtggtatt atcgaaaagg tattaaaagc aaagtctgca
     9181 aagcagatgt ctaaaacaga ggctcgctca aaagggctct tggtagagat tagccaagag
     9241 atagacgatg attatgttgc catggtgaaa tcgcatcttt tgcgttcata tcttttctcc
     9301 gaaatgggta aatcggtcaa tatcgtttac tctcctcttc acggcactgg ggcaagacta
     9361 ttagagcgta taatgaaaga gctgggtttg aatgtcctta ccgtgccaga gcaacgcgaa
     9421 ccggatggcg aattcccaac cgtgtcttac ccaaatcctg aagaatcagc agcgcttgct
     9481 atggcaattg agttaggcaa aaaaactcat gccgatgtgg taatggctac cgacccagat
     9541 gccgataggc ttggtattgc cgttccggac aaagcaggag aattcgtatt ggtaacagga
     9601 aatcagctcg ggtcactgca tctcgattac attgcgctta ctcttaagga aatcaatagc
     9661 atgccaccta gaccggcggc tatccgcacc atcgttacca ccgaattaca aaaggctata
     9721 gctgagaaat atggcataga gagctttgag tgccttactg gattcaaatg gattgcagat
     9781 ctaatgcgac gcttcgagac cgagaatatc gatttcatct atgcaacgga agaatcctat
     9841 ggtcatctca tcgaaaagga agtgcgagac aaggatggta tttccgcagc ggcccttact
     9901 gctgaaatga ccctctattg gagatcacag ggcaaaagcc tcctggaccg ccttgaggat
     9961 ctatacaagg aacacggtta ttacgaggaa aaaggactaa gcttctattt tgaaggtgaa
    10021 caaggcatga ggataatgaa tagcatcatg gaagactata gaaagcaaca gccagatcaa
    10081 tttggagagc ttgcagtaat ctgcactcga gatgtcaaag ccggtaccga gtgggataga
    10141 gatggcagaa ttcgcaaaat cgatctgcct caaagcgatg tgatccaatg gcgcctagaa
    10201 gacggcacgc tactcactgt aaggccatct ggaactgaac ccaagataaa gtactacatt
    10261 ctctgccacg atcagagcag cgaacttagc ttaagcaaag aaatcacaca aaagaaaata
    10321 gctcttatag cagaagcaat tactgcaatg gtcgattcgc atcgcaaatg atcaataatt
    10381 cgacgaccct ctaaaagcaa tgatgatctt gagagattga ccttaaaggc aagcccgtgg
    10441 attttcgaat aatcaaacat gtaacgattt cggttatatc aattgtttgc ttattggtct
    10501 ttcgttgcgc gatgctttcg cttaatcctt attctgacac gaggcctata tcgcttgcag
    10561 aaattgctgc ttatcccaat aataatgagc gatcttttat ggagaacgat ttctatcata
    10621 acctctctat gttgaatagc agtaatttct cattgctttc gatgaaacaa attgaacaac
    10681 tagcgtctag tctttgcatt caaggcatga ctatggattt agacaatagg gcaatggctt
    10741 ctttgttcct tccatcttct ggtcatgttc ttgccatgct catcgacgat caagactccg
    10801 aagaatatca aaatctagat ggaatatcct tgcctgcaag actctcatat aatcgtattg
    10861 cagcattgca tccagagatg aatgcccttc gacctcgata tgaagcattt atcgatgttt
    10921 tttatcgaag attattagaa agcggtatat ctgacttccc tgcaaaatgg ttcgagcaaa
    10981 atgcacaaac atggaatggc aatgtgaccc ttcctggcct ttcctcactg cctcggcttg
    11041 atgagctcga ctattcgcat acattcgcgc tggatatttt tctgcaagat gtggagtatc
    11101 tctcctggaa tattcagcgt ggccctgtta tccattcatt gagcaatggt atcgtagtgg
    11161 ctgctgaaag cggatggctt gggtttccta gaaagagtgc tgctctttcc tatataaaag
    11221 gaggaatatc tccaaaatcg ggtaatggcg taatagtata ttcacctgaa gaacataggt
    11281 attatttata tttccatctc tatgatctct ttgtaaaaaa aggggatatt ctcttgaaag
    11341 gccaagccat aggtcatggg ggaaattcgg gcatcaatgc acgcaaaaaa ggagggggag
    11401 atcatcttca tctcgaaatc ttcgattcaa agagcggacg ttttctaaga aatacacaaa
    11461 ttatcgcgct tttgaaagac tcttcaaaga acaagacagc tgtttcacca aaataatttc
    11521 ttcgctatag tatagcaatg aacaaattat tcctcgttgg cgatgttgga gggacaaaca
    11581 ctaatctagc cttagccaga catgatgagt cgggcatcga attgatcgaa aaggcgcagt
    11641 tttcaactca gaatgaaaag tccctcttgg atccgatcaa gcgttttctt gaatcaaggc
    11701 gcgtaggcgg agctaaccat aagatcgact tttgctgtat ttctggggcc ggtcctgttc
    11761 aggccaataa tagcattcaa ctcaccaatg caccttgggg tatcaaagcc tcggagcttt
    11821 atgaattcct tagagtgcca gtcaaattga taaatgattt cacggcctta tcatatgccg
    11881 tgactattct tgatcataaa gataaggata aggttacaca gcttccacat ccggatggat
    11941 ctatctatga gccccaagac ggtattatgc ttatcattgg ggctggaaca ggtcttggtg
    12001 tcggctttgt ggataggaga ggcgacagtg tgatggtttt cccttccgaa ggtggacatt
    12061 ccgaattgtc ctgtttcgat aggctgagcg attcctttta cgcgtggctt cgcaagaaat
    12121 atgattatgt gccaggtgct gaactagcta tctcaggcca gggcattgca aatatatttg
    12181 aatttatatg cacagaagat cttgcaatga gtatcgtgga aggcgactat gagatttcgc
    12241 aaaaagcgct ggaaaagggc ctctccgata ctgctcgaaa tatccttgat ttaccacgat
    12301 ttgagtggcc ggctcttatt tcacaaaatg ccaggtctga tgcattttgc aacttgacaa
    12361 tggaactgtt cgttcggcta tatgcgctca aggctgccaa cctcgtgtcc atctttttgc
    12421 ctgctggagg agtctggctt gcgggcggta tatcatccaa aaatgaggat tggctcatcg
    12481 aaaagtcaag attcatgcga tggttcgaga ggaattatgc cacccatatt cgcgatatac
    12541 tctgtagaac tccggttttg atcgtgaaga attatgacat ctctctgatg ggtgctgcca
    12601 tagcagcgct tcaattctcc acacatcact aaggaaactc tagatgaaag aacaggattt
    12661 gcacatcgag ctactcgctg acatgaagaa aaagaacatc gacattgccc tcacgctgga
    12721 tattctcaat cgctataatg ctggccaatt cgatcactat gaaaaggtta taccttcggg
    12781 agtgccgcct ctggatggta tccatgtcat agatataagg aacagaagcc ctaacaatat
    12841 tcttttcaaa tatcccaagc aagaatccat tttgaatcta gagcataatg acatattcct
    12901 ccattccgca tttcctgctg gtaataaaaa tatcttcgaa ataagagaaa tgggaaggga
    12961 aatgctttgc tttaccgaga atgccctcga agagataggc aaacatcttt tgccaaaaac
    13021 cgcctatggt gttttgaatg gtggttctgc tacaagttat atcgacatca aaaagaatgg
    13081 ctctatagat gagtctctat tcgaagcagt aaggctgact tttgaagatc tggcaaaaga
    13141 tatagaaaac aaaccaaaag gattgactcc tgcttatatc aaccctgatg gcagtgccgg
    13201 agcaagtttt ctagagctaa agatgcgctc tcgtcttctt attgcaaaag aacttggtca
    13261 tggtagattg tcaagaaaac ctggagcgct tgcatcctct gcatatcaaa aacaacctga
    13321 tttcttgcct ctttttcaga tgacaagtgt gggcaaccat gaagaacttc tcagggctta
    13381 tgaagaaatg cgagtctcgc catatctaag cgaccttgca cacgtagttg gtctagagcc
    13441 ctgtgaatgg gtaagcggaa tacaacctct aatagctgca tatacccatt cggccgaggg
    13501 gctcccaaag cgcgtttttg atcgagctta tggaaagcat aattcttctc ttgcgattcc
    13561 aggtggtcat ggccagagct tcatgatact tgccgacata tatcgaactc ttctaaagca
    13621 aggaatgcgc tatgcaatgc tcagcaatgt ggacaataca ggggcttatc tagatccaat
    13681 agagcttgct atcctggcta tctcaggaaa acccgcgggt tttgattttg cattcagaac
    13741 acctgtggat gttaagggcg gtatactaat ccttgccccc aacggttcaa gaaacgtagt
    13801 agatatcggg cctgcaatag gacttgagga agtagaatgc ctagaaaaat cgggttccgc
    13861 catccttttc aactgcgcta caggtatttt tgatctcgaa tggctagtcc cacatataga
    13921 agagatctcc agagccctcc ctattcgctt ttctgaccag agcaaagacg caggaaacta
    13981 ttcacaagca gaacaggtta cctgggaagt agtctccctt ttgccagatt ttatcgcttt
    14041 tgcagttcaa aaagataggc gctttcttgc cgcaaagatg cttatagaga tgcttctcgc
    14101 aagtggcttt ggtatagaca aaccttctat cgacccaagc ctaaaacagc taggaatgac
    14161 tatgcataat ggacaatcct ggctattaga aaatgtatat ggactaacaa aagtcaatgg
    14221 ccgatggttg cctcaggaat ccgccctatg catataggta tcccatgcat gatattcgat
    14281 atgctctcaa gtgttccttc atccgaggtg agcatattgg ggtttccgca aagatcaact
    14341 tcatcgatag gcattgtcca gatgccttct tcgatccata cagtaccggg ctggacccag
    14401 tcatcggcta gtgctgaagc cataagcgtc ccgtttttat tccaaacctt gaggatttcg
    14461 ccatcttgag caccaatata ggacatatcc ttggggttga tatgaatttg agcgcattcg
    14521 gatcggtctt cttggccaat gcgctgagaa tggatgaagt tcgatacttt tggcgtcaac
    14581 agccggaatt ctctgaaaga ttcttctgtc gagcctttct tcattagacc ttgccaatgc
    14641 aaggtcatat tctggttcca taaaggactt gcaaattcta ttttgccact gttcgttccg
    14701 atcggacaat gcacaggatc ggcaataaaa tcgtccagcc cacctcgcaa taggcccttc
    14761 ttaagaaaga tcccctcgat cctaaaggct tctatatctg caatttccga ttcagcaaga
    14821 aaatgttcta tccattggga ttcagttctc ccttctgtaa agacagatcc cagaccaaaa
    14881 cgctcagata actgtgaaaa gatccaataa tccgaatgcg tgcctgctaa tgccctacat
    14941 atttccggct tatagagcac ataattgcct gcccaaggaa tgccaatatc ttccttttcg
    15001 aatccatccg aaacaggcag aacaatatcg caataccgag ctgaaggggt aagaaatagt
    15061 tcatgacaca cggcaaattc tacactttca aaggccttga tattctttgt tacatttgcg
    15121 ccttgattaa ggaagttccc cccagcagaa tataaagcct taatagagcc aagcccataa
    15181 gaagatggac ttagcacagc atcggcccat cgaagtatag gcactgtctt tgtgaacaac
    15241 tccgcttcta tcccttgaga tttatggttg tcctcccata ggtgggcctc cttagtaaag
    15301 ccctctttga taaaagctcc cttagtcaaa gaggaaggca attcctttag agtcccaact
    15361 ttgattcctg gaaggcagtt gtttatcgat cctgtcgatg caccaagcag cccactattg
    15421 cgagtagcaa gctgtatagc catagtcagc cgaaaagctt cttcgccata tgctaccctt
    15481 tgaatggaat aacccggaat aatcatggct ggcttctcat cccagaagaa ggaggctagt
    15541 ctaatgatca cgttagcatc gacaccacag atagagcttg cccatagagg agtctttggg
    15601 attccatcga tcttcccaag cacataatct gttaacgatt cgaaaccaat cgaatattta
    15661 gagataaatt ctgcatcgaa tcgcgcatct tggagcattt cgaatagcag agcatagccc
    15721 aggacggcat ctgtacccgg aattatgcca atccattcgg cgtttagaat tcttgccgaa
    15781 atactccgcc gtggatcgat agcgatgatg cgcgctcctc tcttttttgc ttcaatcaaa
    15841 taagcaggaa gttcggcgcc aagtcttgcc tcgaatggat ttgagcccca cagaactata
    15901 agattggaat atctaattgt ctctgcatca aatccgctat tcatccttga tgcgcccaaa
    15961 agcctcgaaa gcgcatatgt tgccgcatta catgaataat ttcctttaac atttacgcat
    16021 ccgccaatgg agttgaaaaa tcgacgagcc aaagtctcgc tattgtgcag ggctccagtc
    16081 gaaccactgc tggctatgca gagtacagaa ccaggcccgt ctttggctct gatatcaata
    16141 agtctttgca caatatacga taaagcttca tcccagccaa tttctctaaa ttggcctgag
    16201 cctcttcgcc ctgttcgcag aagaggagtc ttcaatcttc ttggcgacgc atggtttttg
    16261 tgagcgcgaa agcccttgcc gcagggctta atatatcgac ccgcaagcgg attgagatgc
    16321 atacccctta tatggccatc ttcaacttcg gccaaaaggg gacatgcatc tcctccacag
    16381 tcttttccac agaaaaaagg caagagtgcc atgttttgtt attctatcat gagccaaaca
    16441 ctatttcgat cacttcttcc attcgagata ccggatgaaa tacaagtcct ttcttgacat
    16501 gttcagggat ttcatctagg tccttctcgt ttgcagccgg tattattatt tcccttattt
    16561 tgttgcgctt tgccgcaact gtcttttccc gcaatcctcc aacaggtaga acttgcccgg
    16621 tgaggctcag ctccccagtc atagctaatc tatctcttat tcgcttgttg atcacaagcg
    16681 aaagaagagc ggtggccatg gtaattcccg cagaaggtcc atcctttgga gtggctcctt
    16741 ctgggatatg gaggtgtatc tgccttgatt cgaagaattc agcctggatt ccatacttct
    16801 gagcggcaat attccttaca taagtatagg ctattcccgc cgattcctgc ataacaggcc
    16861 ccatctgacc ggtcagtttg aatccttcct taccagggtt agctacggct tcaatgagca
    16921 aagtatcccc tcccaggctg gtccaggcca atccaatagc catgcccggc ttgctggcgc
    16981 gctttagctc ttcgtcacgg aatataggtt tgcccaggta tttttctacc gcatgcttat
    17041 ctattttaaa ttcttcttct tgtctgttct cgatgactat gctctttgcg atttttctat
    17101 gtattttgtc gagagacttt tcgagattcc ggacaccagc ctcgcgagca tagccatcta
    17161 tgattgcgag cagagcacct cttgtgtact tcacttgatt ctttcctagt ccatttcttt
    17221 caagacttct agggatgaga aagtgccgtg caatctctat cttctcaacg tctacatagc
    17281 cgggaagctg aatcacttcc attctatcga gcaggggcct gggaatagtg tccagagtat
    17341 ttgctgtaac aataaagaaa atgttcgaaa tatcgaacgg cagatcgaga taatgatccc
    17401 tgaaactaaa attttgctcc ggatcgagga cttctagcag ggcgctggat ggatctcctt
    17461 ggtaggagat gcccattttg tctatctcat ctatcataaa tacgggatct cggcttttag
    17521 cgatcttgag accttggatg atcttacccg gcaaagcccc cacataggtt ctgcggtgtc
    17581 ctttgatctc agcttcgtcg cgcattcctc ccacagagaa tctaaagaat tgcttgccta
    17641 gagcacgtgc tacagacctg ccaatgcttg tcttaccaac acctggcggc ccaacaagac
    17701 atagaatagt cccacgcgta tcttttttga gcttgcgcac ggctagatat tctattatcc
    17761 gatccttgac atcctgtaga ccatagtggt cctcttcgag tactttcttt gcatgcttga
    17821 tatcgaaatc agcagttatt gatgtattcc agggcaatga acagacaaga tctagccaat
    17881 tccgagtcac catgtattca ctggaagaag gctccatgag gttgaatttt tccagttcct
    17941 gctcgactat ttccttgatc tcgccttcaa actgaaatga atcgatcttt tctctgaagc
    18001 gctgatagtc agaacttctc gcatccgggg ccattccaag ttcttgtttg atagccttga
    18061 gctcttcccg aagaaagtat tctcgctggc ttttttcgat tttttcattg atctcagcct
    18121 ggactttttt ttgaatccta aatagctctt tctctctgtt gatatgaata agaacttttt
    18181 ccatgcgctc gcggacatct agagtttcga ggatgtcttg ctggtctttt ttctcgatat
    18241 tgagtatgct ggtgataaaa tcggcaatct tacctggatt gtcgatattg atcatgttga
    18301 ggcgcatctc ttcagagaag agagggttat tttcgctaag ctgcttcatc tcacttatga
    18361 gtgctcgagt taaagccttt acttcatcgt tgtcataatt ctcgtcgtcc aaataactga
    18421 ctgccgtaat tatcggcggc tccttagaga tgaatttctt aatcttgaaa cgcttgaaag
    18481 tcgatatgaa gatattgatg ccaccgtctg gaagattgat tttttttatg atctttgcag
    18541 cagtaccaac atgaaataaa ttatccgagg acggctcgtc agaatcgtct ttttgaagca
    18601 caagaccaat catcccatca gccgatatgg cctgttccac aagctgaatg tccggctcct
    18661 tgccaaccat tattggcgta aagatgcccg ggaatattgg ttttcctatg agcgttatta
    18721 cgggcagttt accaggcaga atttgatcta ttggtataag ctcgcttgcc gacatattgt
    18781 accacctaat agaattataa tgaaaatata ttaatgtttg cattgcggaa taagaaaccg
    18841 cattacaaat gtacggaaaa ataaggctat tcggcaagga ttctgtgaat ccattgcctt
    18901 taaggtcatt ttgcttgact catctggctg gctcatgcta gtgttcagcc gctatatgaa
    18961 tggattttag ggcgcttggc tcagttggtc agagcggctg gtttacaccc agtaggtcgg
    19021 cggt
//
LOCUS       DAOOWY010000030        18866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_2939_length_18866_cov_11.416512, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000030 DAOOWY010000000
VERSION     DAOOWY010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 18866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18866
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2939_length_18866_cov_11.416512"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PK105_03845"
     CDS             complement(<1..300)
                     /locus_tag="PK105_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HOU60873.1"
                     /translation="MKVLLVGSGAREHALAIAIAASAECERLVISPGNAGMADVGEIA
                     NIGLQDIEGLCSLAQNIPADLVVIGPEAPLAAGLVDRLTERGIACFGPSAGAAQIE"
     gene            complement(297..1709)
                     /gene="purF"
                     /locus_tag="PK105_03850"
     CDS             complement(297..1709)
                     /gene="purF"
                     /locus_tag="PK105_03850"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOU60874.1"
                     /translation="MIGQQEAKESCGVVAVVGEEAARLSFFGLFALQHRGQESAGIAT
                     LDEGNIYLHKDVGLVSQVFRDQNLAGLKGNMAIGHTRYSTTGRPSARNAQPLLIDTQY
                     GPLALGHNGNIANAPELRNQLLARGLGLMTGSDSELLAMMLAGTHGANWTERILSGMR
                     SWIGAFSLVILTHEGVFAARDPWGFRPLAWGRIASGGYAVASETSALRVMGCSELEEI
                     PPGMVLHLTEHGVVERGHADIQAQTAACSFEYVYFSRPDSVWNGKNIHAVRRKLGALL
                     ADEAPAEADLVIPVPDSSIAAAIGFSQRSGISFGEGLVKNRYIGRTFIEPTRALRQQG
                     VALKFSPLRETLEGARIVLVDDSIVRGTTTAQIIALCRRAGAREIHLRIASPRILYPC
                     YMGVDMGTKSDLMAVGRNLSEITKILGADSIAYLSIEGLSNAIGVDGLCKACFDGNYP
                     IPVNEGFQKECFECVSKGAL"
     gene            complement(1706..2233)
                     /locus_tag="PK105_03855"
     CDS             complement(1706..2233)
                     /locus_tag="PK105_03855"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOU60875.1"
                     /translation="MNRVRVIIFAGSPADKAHFETIQKALEVFGIPCLARIASAHKTP
                     KRLLDMIKSYDSEGIPTVYITVAGRSNALSGLVDAATQYPVLACPPPSEQWSAYDIWS
                     SLRMPSGIASALILDPVNAALCAAKLLALKDEALRENIRRFQQQQIDRLIEEDRLIEK
                     NETMEKKPMQERPRP"
     gene            complement(2235..3215)
                     /locus_tag="PK105_03860"
     CDS             complement(2235..3215)
                     /locus_tag="PK105_03860"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOU60876.1"
                     /translation="MVSEDLVRQLLPIAFRGTRKAEGEGKVREWWKLPSNKRLIATTD
                     RISAFDRVLAAVPFKGQVLNELSAFWFRKTEDIIANHLLSLPDPNCAVVKEVKPLPIE
                     LVVRGYITGVTSTALWRRYELGERIIYGQRFPEGMRKNEKLPHPVITPTTKGGPSGHD
                     ERLEPREVLEKGYLDAATWNDVQDAAFALFSTGTDCAERAGLILVDTKYEFGIAPDGS
                     LMLIDEVHTPDSSRFWLASSYQQRFEAGFEPESRDKEFVRLLYAELGYSGDGDIPKLP
                     DSVWIQTSILYQELYEKLTGLTFVPCSYPAEERILRTVEELKEKDAARRT"
     gene            complement(3228..4019)
                     /gene="purQ"
                     /locus_tag="PK105_03865"
     CDS             complement(3228..4019)
                     /gene="purQ"
                     /locus_tag="PK105_03865"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOU60877.1"
                     /translation="MENRKPGVASMKAIILKAPGTNRDYDVQSAIEQAGGYADILPIS
                     RLRSDARLLQNYGMLVIPGGFSYGDALGAGRLFALDFLRFFAEEVSRYVESGRPVLGI
                     CNGFQVLVRAGILPGKQFEPNKFTLAHNESGSFECRWVTLLAPASNSIWTRGIDFVFC
                     PIAHGEGRFRSSSAKALDELEEKGCVALQYADSSGKLAHSNFPENPNGSMRDIAGICN
                     PEGNVLGLMPHPENAVFEWQTLALASPAASGARRLFENGLRYAAL"
     gene            complement(3986..7075)
                     /locus_tag="PK105_03870"
     CDS             complement(3986..7075)
                     /locus_tag="PK105_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOU60878.1"
                     /translation="MANGENLYRFEVRTRGAGSPRIEALKADVRALGMGILRSARDTA
                     LYFVEGDLAPEELCLLGRFLLSDPVEETFEWREVSRQALHGSISEPDTVRIVEICRKP
                     GVTDTVANELVRAAREMGIPCLNRAATGIRWELEADWLNETSLLKLTRVLLANSLIDR
                     WEFGEINPIFPEGQLLVPPVEQYDLGIMDDDSLLSLSSERRLALDLTEMKAIQAWFVA
                     QRREITDVELEMIAQTWSEHCVHKSFKADIEVIRDSKPSDRESAFPRIIHSIFNTYIK
                     NTTEEIGAPWVKSAFVDNAGIVSFDDRWDLSFKVETHNHPSAIEPFGGANTGVGGVIR
                     DIMGVSAKPIAVTDILCFGPPDISEDELPQGTLHPRLVARGVVAGVEDYGNKMGIPTV
                     NGAVHFHPGYAANPLVYCGCIGLAPRGIHRNKPESGDHVIVLGGRTGRDGIRGATFSS
                     MKMDGSTGEVAGASVQIGDPIIQKKALDVLLAARDAGLYNAVTDCGAGGLSSAVGEMA
                     RELGAEIDLSGLGTKYPGLAPWELWLSEAQERMVLAIPPRHIKAFSELCSIFECEFWD
                     IGAFRSDGELKVRVKGELVLDLSMIFLHQGLPKKLLMAKEPLSKSPQERLQKSIEANV
                     YQARLDTLGIQTTEASNVHSELQGPDENRQASPGLIELKEQLLKLLGSREIASREKIV
                     RRYDHEVQGNSVIKPFVGAFYDSPSDAAVLKPKGAACEWGIALSNGFRSDYGELDPYR
                     GAWAAVDEAMRNIVASGGDPSRTAILDNFCMGDPNDPGVMWSLLESARGLREAALAFH
                     TPIISGKDSFYNEYLGADGSRHAVPPSLLISALAYVPKVERVVTTFFKHPADSIWLVG
                     DFKPSAIWQEEVPEISLRAQEVYSSLFDAIQEGEVLAAHDLSEGGLAVALAEMCIGGR
                     LGARIALSDIYEQRNIPISETTETDNAILDVLRLALFGETMGCLLVEVGAGKEEQFAA
                     RFAPNSSKTSLEQNNLAKRIGEVLKEPSLEIVDPFLFLVSIGEMLDAWKTASQELLP"
     gene            complement(7296..8573)
                     /locus_tag="PK105_03875"
     CDS             complement(7296..8573)
                     /locus_tag="PK105_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOU60879.1"
                     /translation="MDLFVLILLIVMYLGIILLPSKKAWISMGASVVLILGGAVTPAH
                     AFGSLVNWNILLIYLGSLILAELFIYSRVPAFIAQRIVERSPSIGIAITIILFLTGLL
                     SAFVENVATVLVIAPIMIELAKRGKRNLAPLMIGLAVMANLQGTATLVGDPPSMLFAN
                     FAGYSFNDFFFKSGKPSIFFAIQIGAIVGALYFYFYFRKTDSDIKVLPPEGIVAWFPS
                     VLLLSMIGGLALFSALSAQGGIHPASGIFVFVLALVGLAWLVLAKKEKEEAKNLIIHL
                     DWMTLVFLIGIFIVVGALAESSLLNQLAEQLAQWVKGDIFLGFALIVAISVLISGFVD
                     NVPYIAAMLPVANALANAMQIQPDLLMFGLLIGSCLGGNLTPFGASANIVAVGLSEKH
                     GCKVSFWRWIKLAGPFTIITTIAASAFVWLVWA"
     gene            9041..10123
                     /gene="ilvE"
                     /locus_tag="PK105_03880"
     CDS             9041..10123
                     /gene="ilvE"
                     /locus_tag="PK105_03880"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR01123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOU60880.1"
                     /translation="MSRFESMVQSGTIVPTALSAIDWNKLGFGGIITPFIGGSVALSD
                     GIFESISIVANGNISIPPLACCLNYGQELFEGMKANRGPDGRIRLFRIDANAARMAKG
                     AARFMLAAPSEELYRKAIIETVKANADYVPPYGKGALYVRPILAGVGMTLNPAPSDTT
                     IFLVTTVPVGMHYKGSSMVSIKVETDFQRAAAKGTGWVKAAGNYAPCFLPTSEAKHKG
                     FDDLLFLNQTGTHVEEVGTANFAMIKHGILYVADSPSILPGITRDSVMRIARELLDIE
                     VVFAPLELDRVLGLKEFAGEGPADEAFFTGTAAIISPISNINYEGKDYCLGENVGPFA
                     TKLREVLLGIQTGVMPDPFDWVTIIE"
     gene            10397..10469
                     /locus_tag="PK105_03885"
     tRNA            10397..10469
                     /locus_tag="PK105_03885"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10430..10432,aa:Ala,seq:cgc)
     gene            complement(10711..12108)
                     /gene="lysA"
                     /locus_tag="PK105_03890"
     CDS             complement(10711..12108)
                     /gene="lysA"
                     /locus_tag="PK105_03890"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOU60881.1"
                     /translation="MKDTPFSPAILIDGSKCYFDGVDLRYLAHEFGTPLYVMSETIIR
                     ERCEELRSAFLCRYPGTRAYYASKALQTLDVLKIIKEEGLGVDVVSGGELHAALNAGV
                     TPSDIIFHGNAKTDEELHYAISSKVGLIAVDNIEEIFRIEEEADRLSRDQRVILRVSP
                     GVDSHTHRFISTGSLDSKFGLPIEHSEVERYVKALALCPHVEFSGLHFHVGSQLLENT
                     SHLAALEIVLNFAAKLSKEFGLQVKEINFGGGFGIRYLPSDRAPALLDFIDPMMSMVK
                     EWSRKENLPMPVCAIEPGRWIVGEAGITLYKVIAVKEIPGVRTYVSVDGGMGDNIRPA
                     LYDARYHVETVSQACAMVPCEKEKNCSKVEVLRERDAEKALGMDSNREPILRKVTIAG
                     RYCESGDIIAEDVFLPDPKPLDILAVYSTGAYCFSMSSNYNRVPRPAMVITKSGDYRL
                     SVRRETYDDLLRREI"
     gene            12206..12943
                     /locus_tag="PK105_03895"
     CDS             12206..12943
                     /locus_tag="PK105_03895"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOU60882.1"
                     /translation="MKKPLIGITSSVDRSRQPVAYFSLKESYVKAIEMAGGTPLILAV
                     TSSAVMKALTSILDGLIFSGGGDIAPWLFDQEPILGLGSFDTLRDEWEIELCNQAWKA
                     RIPMLGICRGCQLMNVARGGSLLQDIERSNPAALLHNPQIAYDELFHHIAIEKESVLF
                     ELFKTERLLVNSFHHQAVDKTASDFRITARSSDGIIEALEPIDPERFALALQFHPEGL
                     FLRYGAFLAPFSALIEATKGSLSATRR"
     gene            complement(13018..14241)
                     /locus_tag="PK105_03900"
     CDS             complement(13018..14241)
                     /locus_tag="PK105_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOU60883.1"
                     /translation="MAQWKNLAFLQKEVFFESRAMEEWLIEMRRSIHRIPEPGNKEQK
                     TSSFICKMLEEMDIPHRSYGTAVVGLIEGKEPGRCVALRADMDALPLEEPSDRPYASL
                     HHGYMHACGHDAHMTVALGVARLLAAHRRDFSGSVKFLFQPAEETTGGAKAMIEAGCL
                     ENPHVDLVLGLHVVPELMAGMVEVKIGPFYGTSDSLEIIVKGRSSHAAYPEQGVDAIV
                     VSASIIQALQTIVSRSISALDNAVLTIGKIQGGVRSNILANEVIMIGTIRALSEDVQC
                     FMRTRVKEICTEIAAAFGASCEVHIRPSYPVMINDQSATELLRDTAISILGESNVIIR
                     QQPNLGVEDFAYFLRERPGTFWHLGCGKPGMEKIHPLHSAEFDIDESCLPIGVAIQAA
                     SALTFLHLRNQESCG"
     gene            complement(14243..15166)
                     /gene="dapF"
                     /locus_tag="PK105_03905"
     CDS             complement(14243..15166)
                     /gene="dapF"
                     /locus_tag="PK105_03905"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOU60884.1"
                     /translation="MKILQQGFHAHKYQALGNDYLVIDPAEFQMSFSASAISALCDRN
                     RGVGSDGILYGPILSQTSSVICSPFGLRIFNPDGSEAEKSGNGLRIFSLYLYERGFVG
                     KEPFEVQTKGGMVECRILATNSPCSGEDGYRKDDHREEGYEEEHYIEVSMGEARFLPG
                     SSTLAIDGIEFRTIRVSMGNPHCVLLGQYPTEELARRIGPLVENHPDFPNRTNVQFLE
                     VIDRQTIRIEIWERGAGYTLASGSSSCAAAAAARRLGLVENEVRVRMAGGTLYIDMRE
                     PNIKMTGPAEHSFDLVFSPSLMRLAMKDKGD"
     gene            complement(15169..16293)
                     /gene="alr"
                     /locus_tag="PK105_03910"
     CDS             complement(15169..16293)
                     /gene="alr"
                     /locus_tag="PK105_03910"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOU60885.1"
                     /translation="MDHRATTAWIDLAAIRANFRHLRDIAQGSIVCPVIKADAYGHGA
                     LAIAHELEREGAQFCSVALAKEALDLRESGIQMPLLIFGAPEHSSSSLAIRLECAQTI
                     YTEEHVRILASAAKSVGKKAKAHIKIDTGMNRQGLPWTKARSFAGLLADYPEIFVEGI
                     YSHFADADSPDPSFTDLQMKRFSIALESFGRVGITPKYRHIANSAGMVLYPKTRLDMV
                     RPGILLYGLRPAPNIELPEGFEPALALTTGIVHIREVPAEESISYGRTFTTKRFSRIG
                     LMPIGYADGYPRALSNRASVLIRGVRAPVVGRVCMDMTMIDLTGIAGARVGDEVLLFG
                     GRTLPVSEISNLLSTIDYEVVCMISKRVPRVHIDSKPQEE"
     gene            complement(16297..17571)
                     /locus_tag="PK105_03915"
     CDS             complement(16297..17571)
                     /locus_tag="PK105_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU60886.1"
                     /translation="MTYNHYDDTYILEGADLVLPDSILPKATLAVRGGKIAAIIPSGQ
                     FQRPEELNRSSQSQIDPAFENAPVIKAEDAFVLPSLAEMHIHGCASWGFERISEAGDL
                     FQIAALLEAKGVGCYVPTILWEERTLIELTETILASGISAPLLPGIYLEGPFVNHSKR
                     GGIMPDNIRAPDIALAEHILDISKGLLKICTMAPELDGIDQIYPVFQNKAVLVSLGHS
                     DAKLSSIHPPSRPFSITHLFNAMTGIDHREGGLANFAFSQSPDYVELNGDGIHVNESC
                     LKLCSRLIPENSLILISDAVIGAGLPYGEFSYYGRRVVSSERGVRYADSGVLMGSNML
                     GIDIVKNYYLKAEVPLWRAVRAMSLTPRKALGMDGEYGSIEVGKIADIFIWDKGLETA
                     VRPKALLASENDYKPNDLLSSKFKVHRVQKKD"
     assembly_gap    17634..17733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17734..18660
                     /locus_tag="PK105_03920"
     CDS             <17734..18660
                     /locus_tag="PK105_03920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU60887.1"
                     /translation="PCSSLIRDISYILLQLLIHKVRSSNDQLPTFIYYHHMTASKNLD
                     PSKLRFIAMDMDGTILDEGYQLSPHVADTLKKLQNQGKKLIIATGRIYKAAKIVLADS
                     FEPDAFVCTNGADIYNRDGTILASHHIPTEALPILTKVSRDHDVLFCCYVGERWIYDR
                     YSPGVDFYEKRTGMKGVRKNFDSLVGEDILKFLAVGPNEKLLEMRETILNSAPDLLDA
                     FFSHETMLEIVASGVSKRQGLEECLHYLGGSLEETIAFGDAENDLEMLKSVKVGVAMG
                     NAHSYIQTAVPYVTASVDDDGVARFLEELFGI"
BASE COUNT         4923 a         4835 c         4173 g         4835 t
ORIGIN      
        1 ctcgatttgc gctgctccgg ccgatggtcc aaagcaagct attccacgct ctgttagccg
       61 atcgacaagg cctgcggcta gaggtgcctc tggcccaatc acaacaagat cggcgggaat
      121 gttctgtgca agagaacaga gcccttcgat atcctgcaga ccgatattcg ctatctcgcc
      181 aacatccgcc atgcctgcat tgcctggtga aattaccagt ctttcacatt ccgctgaagc
      241 cgctatcgct attgcgagcg catgttctct cgcccctgaa ccgacgagca gcactttcat
      301 aatgccccct tacttacaca ctcgaagcat tctttttgaa agccctcgtt tactggtatg
      361 gggtaatttc catcgaaaca agctttacac aggccatcta cgccaatagc atttgaaagc
      421 ccttctatac tgagatatgc aatggagtcg gctccaagaa tctttgttat ctcagaaaga
      481 ttccttccta ccgccataag gtcactcttg gtccccatat cgacacccat atagcaagga
      541 tagagaattc tcggagaggc tatcctgaga tgtatctcgc gagctcccgc tcttcggcag
      601 agcgcaatta tctgagccgt tgttgtaccg cggactattg aatcatcgac tagcacaatc
      661 cgagctcctt caagggtttc tctgagcggg ctgaatttta gcgctactcc ctgctgacgc
      721 aaggctcttg tgggctcgat gaaggtacgc ccaatatatc tgtttttaac aagtccttcc
      781 ccaaaggaaa tacctgaccg ctgagaaaat cctatggctg cagcaatcga agaatcaggc
      841 acaggaatga caagatcggc ttctgctggt gcctcgtcag ccagtaaagc accgagcttg
      901 cgacgaacag catgaatatt cttgccattc cacacagaat ctggccttga aaagtaaaca
      961 tattcaaagc tgcatgctgc tgtttgggcc tgaatatcag catggccgcg ctcgacaact
     1021 ccatgctctg tgaggtggag taccataccc ggcggaatct cctcgagttc cgaacagccc
     1081 ataactctga gagccgaggt ttccgaagca acagcatagc cacccgaagc tattctaccc
     1141 caagcgagcg gcctaaagcc ccagggatcc cgggccgcaa agactccctc atgcgtcaag
     1201 ataacgagcg aaaatgcacc tatccaggat cgcattccac ttaggattct ttctgtccaa
     1261 tttgctccat gagttcccgc aagcatcatg gccagaagct cgctgtccga gcctgtcatt
     1321 agaccaagcc ctcttgcaag cagctgattc cttagctccg gagcattggc tatattgccg
     1381 ttatgcccta atgcgagagg cccgtactgt gtatcgatta gaagaggctg ggcgttgcgg
     1441 gcgcttggcc ttcctgtggt tgagtaacga gtatggccta ttgccatatt gcccttaagg
     1501 cctgcaagat tctggtctct gaagacctgt gatacaagac ccacatcttt gtggagataa
     1561 atgttcccct catcgagtgt ggcgatgcct gcagattcct ggccacgatg ctgaagggca
     1621 aagagaccaa aaaaggacaa tctcgccgct tcttctccca cgaccgccac tacgccacag
     1681 ctctccttgg cctcttgctg tcctatcatg ggcggggcct ctcctgcata ggctttttct
     1741 ccatagtctc gttcttttca atgagccgat cttcttcgat aagtcgatcg atctgctgtt
     1801 gctggaatct gcgaatgttt tcgcgcaatg cctcgtcttt cagagcgagc agcttggcgg
     1861 cgcagagagc cgcatttact ggatctagaa ttagagcgga agctatgcca gaaggcatac
     1921 gaaggctcga ccaaatatcg tatgcagacc actgctccga aggaggtgga caagctagaa
     1981 caggatactg agttgccgca tcgactaagc ccgataatgc attagatctg cctgcaacag
     2041 ttatatacac agtgggaatt ccctctgaat cgtaactctt tatcatatcg agaagccttt
     2101 ttggggtctt gtgcgcggat gcgatgcgcg ccaggcatgg aatcccaaat acttcgagag
     2161 ccttctggat tgtttcgaaa tgtgctttgt ctgcaggcga tcctgcaaaa atgataactc
     2221 tgacgcgatt catcttatgt cctccgagcg gcgtcctttt cttttagctc ttccacggtt
     2281 cgcagaatgc gctcttccgc agggtaagaa cacggcacaa aggtcaggcc ggttagtttt
     2341 tcatataatt cttgatacaa tatactagtt tgaatccata ccgaatcagg aagcttgggt
     2401 atatcaccgt cgccactata gcccaactcc gcatacaaga ggcgaacgaa ttccttgtct
     2461 ctgctttctg gctcaaaccc tgcttcgaat cgttgctgat agctagaagc aagccagaaa
     2521 cgcgaagaat caggcgtgtg gacttcgtcg atcagcatga gcgaaccgtc gggagcaatg
     2581 ccgaattcat atttggtatc caccaggata agtcctgcgc gctccgcaca atccgttcca
     2641 gtagaaaaaa gagcaaaagc cgcatcctgg acatcattcc atgtggcagc atccaggtag
     2701 cctttttcga gaacttctcg aggttcgagc ctttcgtcgt ggccagaagg gcctcctttt
     2761 gttgtgggtg tgatgactgg atgcggaagc ttctcattct tgcgcatacc ttcgggaaaa
     2821 cgctgcccat agattatgcg ttctcctagc tcataccgcc tccagagagc agtggaagtg
     2881 actccagtta tatagcccct gaccaccaat tcgatcggga gcggcttgac ctctttgacc
     2941 acagcgcaat taggatcagg caaagaaagt aggtgattgg caatgatgtc ctcagtcttt
     3001 ctaaaccaga aggctgaaag ctcattgagt acctgcccct taaaaggcac ggccgcaagt
     3061 accctatcga aggcggagat tctatctgtg gttgctataa gccttttgtt gctgggcagt
     3121 ttccaccatt ctctgacctt gccctctcct tcggcttttc ttgttcctcg aaaagcaatt
     3181 ggaagaagct gccttactag atcttccgaa accatgctgc ctccttttta caaagccgca
     3241 taacgcaatc cattttcaaa aagcctgcgt gcgccactcg cagcgggaga ggccagggct
     3301 agagtctgcc actcgaaaac cgcattctca ggatgaggca ttagtccgag cacattgcct
     3361 tccggattgc agatgcccgc aatgtctctc atagatccat tgggattctc cgggaaatta
     3421 ctatgggcaa gcttccccga agaatctgca tattgcagag cgacgcagcc cttttcctcc
     3481 agctcatcga gggccttggc cgagctggag cggaagcgtc cttcgccatg agctatcggg
     3541 cagaaaacaa aatctattcc ccttgtccaa atcgaattgg aagctggcgc caaaagagta
     3601 acccatctgc actcgaaact cccgctctca ttgtgcgcaa gagtaaattt gttgggttca
     3661 aattgtttgc ctggtaaaat tcccgctctc actaatacct gaaagccatt gcatatacct
     3721 agcaccggcc tgccagattc tacatagcgg ctcacttctt ccgcaaagaa gcgcaagaag
     3781 tctagagcaa acagccttcc agcccctaat gcatctccat aagaaaagcc tccaggaatg
     3841 accagcatac catagttctg tagtagcctt gcatcgctac gaaggcgaga gatgggcaga
     3901 atatctgcat agcctccagc ttgttctatt gccgactgaa catcataatc tcgatttgtg
     3961 cctggagcct tgagaataat ggctttcatg gaagcaactc ctggcttgcg gttttccatg
     4021 cgtcgagcat ttctcctata gaaaccaaaa aaagaaatgg atccacgatt tcgagagaag
     4081 gttccttcaa aacttcgcca atcctcttag ccagattatt ttgttccaag cttgtcttcg
     4141 aagaattagg agcaaatcgc gccgcaaact gctcttcttt cccagcaccg acctcgacca
     4201 gaagacaacc catcgtctca ccaaataggg cgagccgtaa aacatcaaga atagcattat
     4261 cggtttccgt ggtctcactt atgggaatat tcctttgctc atagatatcg gacagcgcga
     4321 tccgagcgcc tagccttcct ccaatacaca tctccgcaag agctactgca aggcctcctt
     4381 ctgaaagatc gtgtgcagcg agcacttcac cttcttgaat cgcatcaaat agggaagaat
     4441 agacctcttg cgctcttagg ctgatttcag gcacttcctc ttgccaaatt gcgctaggct
     4501 taaagtctcc cacaagccag atcgaatcgg ctgggtgctt gaaaaaagtg gtcactaccc
     4561 gttccacctt gggcacataa gccaaagccg agattagtaa agatggcgga accgcgtgcc
     4621 tgcttccatc tgcgcccagg tattcgttgt agaaagagtc tttaccggag ataattggag
     4681 tatgaaaggc taaggcagcc tctcttaagc cccgtgcact ctccagaagc gaccacatta
     4741 ctccaggatc attcgggtct cccatgcaaa aattatcgag aattgctgtc cggctcggat
     4801 cgccgccact tgccactata ttccgcatgg cttcgtccac cgcagcccat gcccctcgat
     4861 atggatccag ttccccataa tccgacctaa aaccattgga aagcgctatt ccccattcac
     4921 aggctgcccc tttaggcttc agaaccgcgg cgtccgaagg cgaatcgtag aaagctccca
     4981 cgaatggctt gataacgcta ttgccttgca cttcatgatc ataacggcgc actattttct
     5041 cgcgcgatgc aatttctctg ctgccgagca gctttagaag ttgctctttg agttcgatca
     5101 gtcctggact cgcttgtctg ttctcatccg gaccctgcaa ttccgaatga acattggagg
     5161 cctcggtggt ctgtatgccc agggtatcaa gcctagcttg atacacattt gcttctatcg
     5221 atttttgtag ccgttcttgt ggcgattttg atagcggttc tttcgccata agcagctttt
     5281 tgggcaagcc ttgatgcaag aatatcatgc tcagatcgag tacaagctcg cccttcaccc
     5341 tcactttcag ttctccatcg cttctaaatg ccccgatatc ccagaattcg cattcaaaaa
     5401 tactgcatag ctccgagaaa gccttgatat gccgaggagg tatggctaat accatgcgtt
     5461 cttgagcctc ggaaagccag agctcccagg gggcaaggcc tggatatttg gtgccaagac
     5521 cagatagatc gatttccgcc ccgagctctc ttgccatttc accaacagca gaagaaagcc
     5581 ctcctgcccc gcagtccgta actgcgttgt atagtcctgc atcccgcgca gccaggagca
     5641 catccagagc ttttttttgg atgataggat ctcctatctg taccgatgcg ccggcaacct
     5701 ctccggtaga accatccatc ttcatcgaag aaaaagtggc tccgcgaatt ccatctcggc
     5761 ctgtacgccc acccaagact atcacatgat cgcctgactc tggtttattt cgatgtatgc
     5821 ctcgaggcgc aagtccaatg caaccacagt acacaagcgg atttgccgca tagccaggat
     5881 gaaaatgaac cgctccgttg acagttggaa tacccatctt gttgccataa tcctcgacac
     5941 ccgctacaac gcctcgcgct acgaggcgcg gatgaagcgt cccctgaggt agttcgtctt
     6001 cggatatatc gggaggtcca aagcaaagaa tatcggttac tgctatgggc tttgcggaaa
     6061 cacccataat atcacgaata acgcctccta caccggtatt agcgccccca aatggttcaa
     6121 ttgcagaagg atggttatgt gtttctactt tgaatgaaag gtcccatcgg tcatcaaaac
     6181 tcacaatacc ggcattatcg acaaatgcgg actttaccca cggagctccg atttcctcgg
     6241 tggtattctt aatataagtg ttgaagatac tatgtataat tcttggaaac gccgattctc
     6301 tatcgcttgg tttggaatct cttattacct cgatgtcggc tttgaagctc ttgtgtacgc
     6361 aatgttcgct ccatgtctgg gcaatcattt caagctcaac atcggtaatc tcgcgtcttt
     6421 gtgcaacaaa ccaagcctgg atggccttca tctcggtaag atcgagtgcc aatcgcctct
     6481 cggaagatag agagagcaga gaatcatcat ccatgattcc aagatcgtat tgctcaaccg
     6541 ggggaacaag aagctgtccc tctgggaaaa tgggattgat ttccccaaac tcccatcgat
     6601 caatcaacga attcgccaag agcactctgg tgagcttcaa tagtgaggtt tcatttagcc
     6661 aatctgcctc cagctcccaa cggatacctg tcgcagccct attgagacat gggattccca
     6721 tctctctcgc tgctcttaca agctcatttg cgaccgtatc agtaacgcct ggttttcggc
     6781 aaatctccac aatccggact gtatctggct ccgagatcga gccatgtaac gcttgtcgcg
     6841 acacctctcg ccattcaaaa gtctcctcta ccggatcgga taaaagaaaa cgcccgagaa
     6901 gacaaagctc ctcgggcgcc aaatcgcctt ccacaaagta cagtgccgtg tcgcgcgcac
     6961 tgcgcaatat ccccatccct agcgcgcgta catccgcttt caatgcttct atgcgcgggc
     7021 ttccggctcc tctggttcgg acctcgaacc tatagagatt ttctccgtta gccatactac
     7081 tatctagcgc gatatggata tattgtcaag ccgccaagaa atcttaggca atggtggctt
     7141 ttttaatttg gaccaataat cgctttataa tacttgccaa ttccggcctt ttgaaattag
     7201 cgaccgatag tcttttctgg aatattggta aacagccgat tttatcatgc gcctttgacc
     7261 caatatatgg caacctataa ccctgggaaa taacctcaag cccacacaag ccagacaaag
     7321 gccgatgccg caatagtcgt tataatagta aagggccctg cgagcttgat ccatctccag
     7381 aaactaacct tgcatccatg tttttccgaa agtcctactg caactatgtt agccgaagcc
     7441 ccaaaaggtg taagatttcc accgagacaa gacccaataa ggaggccaaa catcaacagg
     7501 tcgggctgaa tctgcattgc atttgcaagc gcattcgcaa ccggaagcat cgcggcaata
     7561 tatggaacat tgtcaacaaa tcccgaaata agcacagata ttgctacaat tagcgcaaaa
     7621 cccaaaaaga tgtcaccctt aacccattga gccagctgtt ctgctagctg atttaggaga
     7681 ctcgattcag caagtgctcc caccacaatg aaaatgccaa tgagaaaaac aagtgtcatc
     7741 cagtctagat ggataatgag gttcttggct tcttccttct ctttttttgc aaggacaagc
     7801 catgccaacc ccacaagcgc aagcacaaag acgaatattc cactagcagg gtggatccct
     7861 ccttgagcag aaagcgcaga aaagagagcg aggcctccga tcatagacaa aagaagcaca
     7921 gatggaaacc aagcaacaat gccctctggc ggcaagactt tgatatcgct atcagttttt
     7981 ctgaaataga aatagaaata tagtgctccg actattgcac caatctggat agcaaaaaag
     8041 atcgatggct taccagattt gaaaaagaag tcgttgaatg agtagccagc aaaattagca
     8101 aagagcatag aaggaggatc gcccaccaaa gtcgctgtac cctgcagatt agccattaca
     8161 gcaagtccaa tcatgagagg agcaaggttt ctcttacccc gctttgccaa ctcgatcatg
     8221 ataggcgcaa taacgagtac cgtcgcaaca ttctcgacaa atgcggatag cagacccgtc
     8281 aaaaagagaa taatcgttat ggctatacct atcgaaggcg aacgttcaac aattcgctgg
     8341 gctataaaag caggaaccct cgaatagatg aaaagctcgg caagtattag gctccccaaa
     8401 tagattaaca gaatattcca gtttacaagc gaaccaaagg catgtgcagg agtaaccgca
     8461 cctcccaaga tgagcacaac agaggccccc atagaaatcc atgctttttt tgaaggcagg
     8521 agtatgattc ctagatacat cactatgagc aaaatgagca caaaaaggtc caaggtatcc
     8581 tcctttgaaa agcccaatgg catttcgcct gcagccaact tcttagccgt ttttggtcac
     8641 tgtacagtca aaaaaaagac cgcacggcca tgacaccgtg cggtcttgct caatcgtttt
     8701 ggatctgcga aaaccggaag caaagctccg tcttgacgat cttcgcacct gctcttcaaa
     8761 tgcaggaagc tactccccgc tccgctaaaa atactccatt cataaaatgc cagtcaatag
     8821 aaaatatgac acctcgatca tcttttcata atatgcctag ttgtattttt gaagatatgt
     8881 agatagctca gttttttagc tcttcataag actaatttac aaccaaatgt gccgaattga
     8941 gcattgcata tatattagct gattttgcat tttattgaac tgtcctattg tctcgattgc
     9001 tcattatgcg ttatcattcg aaaatttttg ggaggttctt atgagtcgat ttgaatcaat
     9061 ggtccaatct ggcacaatcg tccctacagc cctgtctgca atcgactgga acaagcttgg
     9121 ttttggagga ataatcacac cattcattgg aggaagcgta gctttatccg atggaatctt
     9181 cgaatccata tcgatagttg ccaatggcaa catatctatt cctccccttg cttgctgcct
     9241 caactatggc caggaactct tcgaaggaat gaaggccaac cgcggaccag atggccggat
     9301 cagactattt cgtatcgatg caaatgcggc gcgcatggca aaaggagccg cgcgctttat
     9361 gcttgcagct ccttcagaag agctgtatcg caaagccatt atcgaaacag ttaaagcgaa
     9421 cgccgactat gtgccgccat acggcaaagg agccctatat gtaagaccaa tcctggccgg
     9481 agtcggtatg accctcaatc ccgctccatc ggatacgact attttccttg tcaccactgt
     9541 tccggttggc atgcattaca agggaagttc tatggtcagc ataaaagtcg agacagactt
     9601 ccagcgagcc gctgcaaagg gcactggctg ggtaaaagcc gcaggaaact acgcaccttg
     9661 ttttcttccg acttctgagg caaagcacaa aggtttcgat gatttgctct tcctcaacca
     9721 gaccggcacc catgttgaag aagtgggcac cgcaaatttt gctatgataa agcatggcat
     9781 actgtatgtt gcagactctc cttcgatcct tcctggtatt acccgcgatt cggtgatgcg
     9841 catcgccaga gaattgctag acatcgaggt tgtttttgcg cctctcgagc tagatcgtgt
     9901 cctcggacta aaggagtttg ctggcgaggg gcctgcggac gaagctttct ttacgggtac
     9961 tgcagccata ataagcccta tatctaatat caactatgaa ggaaaagact attgccttgg
    10021 agagaatgtt ggtccatttg cgactaaact gcgcgaagtt ctgcttggaa ttcagactgg
    10081 agtcatgccc gaccccttcg actgggtaac cataatcgaa taataagcta atagaagaca
    10141 aacaagtaaa aaggcaaaag ctaaagaacc caagagagaa aaaatgccag aaagcccaat
    10201 atggaggaca ttgccccttg atagatgctt atgaaaagac cataaacagt gcaagagcga
    10261 ctagctcttg attctcgcct ttgctctttg ctatattcta tagtcgccaa tggtagagaa
    10321 cgcggaaagc aattagctgc ctaatagatc tgtggaactt gccatatctt tgtcttgctt
    10381 tttccaaggt ctttacgggg aattagctca gctggtagag cgataggttc gcaatctata
    10441 ggtcaggggt tcgaatcccc tattctccat taactctggg acgaaaacaa gggagcatat
    10501 tctactcctc ggtcggcgtt ctctatcgct caagagcttg agctcgatgc gaaaaagtac
    10561 ccttacaact cgatataatg agcccatctg tctggacaga tgggctcatt ataaacagcg
    10621 ttgtacagga atgccgtaat atttccatta tctctaagcc aagcagatat aattgcgcaa
    10681 ctttttctgc cacaacatta gttatgcctt tcagatttct cttctcaata gatcatcgta
    10741 tgtttcgcgc cttaccgaca gccgataatc cccacttttt gtaattacca tggcgggacg
    10801 cggcactctg ttgtaattgc tcgacattga aaaacaatag gcgcctgtcg aatagacagc
    10861 aaggatatcg agcggcttgg gatcaggcaa gaatacatct tctgcaatta tgtcacctga
    10921 ctcacagtag cgacccgcaa tcgtcacctt tcgcaagatt ggctctctat tcgagtccat
    10981 acctaaggct ttttcagcat ctctctccct taaaacctcg accttggagc aattcttctc
    11041 cttctcgcaa ggaaccattg cacaagcctg gctcacggtt tctacatggt atcgagcatc
    11101 atagagtgca ggcctgatat tatcgcccat tccaccatct acggagacat aggtgcgcac
    11161 accaggtatt tctttgacag caataacttt atagagcgtt attccggctt ctcctacaat
    11221 ccatcgtcct ggttcgattg cgcagaccgg catgggtaga ttttctttcc gactccactc
    11281 ttttaccatc gacatcatgg gatcaataaa atcaagaaga gcgggcgccc tatcggaagg
    11341 gagatagcga ataccaaagc ctcctccaaa atttatctcc ttcacctgaa gtccgaattc
    11401 cttcgaaagt ttcgcggcaa aattcaatac tatttcaagc gctgccagat gcgaagtatt
    11461 ttctaggagt tgcgagccga catggaaatg taagcccgag aattcgacat gagggcacag
    11521 agcgagagcc tttacatatc gctcaacttc cgaatgctca attggaaggc caaacttaga
    11581 atcgaggctt ccagtagaaa tgaatcgatg ggtgtgactg tctactccag gcgaaacgcg
    11641 aagaattacc cgctgatctc tactgagtct atctgcctcc tcctcgatcc taaaaatctc
    11701 ctcgatgtta tcgacggcga tcagtcccac cttagagcta atggcataat gtagctcctc
    11761 gtctgttttt gcattcccat gaaatatgat atcggaggga gtaactccag cattcagtgc
    11821 cgcatggagc tctcctcccg atacgacatc gactccaaga ccttcttctt ttattatctt
    11881 gagcacatcg agagtctgca acgccttgct tgcgtaatag gctcgagttc ctgggtagcg
    11941 gcaaagaaaa gcactacgga gctcttcaca gcgttctcgg ataatggttt ccgacatcac
    12001 atatagcgga gtgccaaact catgagccag atatctaaga tcgacaccgt cgaaatagca
    12061 cttagagcca tcgataagaa tagcggggga aaaaggcgta tctttcattg cttcctccat
    12121 ttccacttta ctgacccttt tctgattatg ctagcacttt gagcctccac tatgcctggc
    12181 aaaccggatt gactacaata actgtatgaa aaagcctctt ataggtatta cgagctctgt
    12241 ggatcggtct cggcaaccag tggcctattt ttctctcaaa gaatcttatg tcaaagcaat
    12301 agagatggct ggcggcacgc ctttgattct agctgttacg agttcagctg ttatgaaggc
    12361 gctcacttct attctggatg gtctgatctt ttcaggcgga ggcgatattg caccttggct
    12421 ttttgaccaa gaaccaattt taggccttgg ttctttcgat accctgcgag acgaatggga
    12481 gatcgagctc tgcaatcaag catggaaagc aagaataccc atgctgggga tttgtagggg
    12541 ttgtcagctt atgaatgtag cccgtggcgg aagcctcttg caggatatcg agcgcagcaa
    12601 tccagcggcg cttttacaca atccgcagat agcttacgat gaactgttcc atcacatagc
    12661 catcgaaaaa gaaagcgttc tgttcgagct tttcaaaacc gaaaggctcc tggtcaatag
    12721 ttttcatcac caggcagtgg acaaaaccgc gagcgatttt agaatcacgg cgcgcagctc
    12781 agatggcata atcgaagcat tggaaccaat agatcctgag cgattcgctc tagcgctgca
    12841 atttcatcct gaagggcttt ttcttcgcta tggtgcattc cttgcgcctt tctccgcttt
    12901 gatagaggct acaaagggat ctttatcggc gactcgtcgt tgagtcctcc ttgagctatt
    12961 cctgaattat tcttgagtta ttcttgagcc attccttatg aattcctagt tatacactta
    13021 tccgcatgat tcctgattac gcaaatgcag aaaagtcagc gctgaagccg cttgaattgc
    13081 gacacctatg ggcagacacg attcatctat atcgaattca gcggaatgaa gcgggtggat
    13141 tttctccatt cccggctttc cacagcccag atgccaaaaa gtccccggcc tctctctaag
    13201 aaaatatgca aagtcttcaa cgccaagatt aggctgttgg cgaataatca cattggactc
    13261 tcccagaatg gatatggcag tatcgcgcag gagctctgtc gcgctctggt catttatcat
    13321 aacaggatag ctcggacgaa tatggacctc gcaggaagca ccaaaggctg cggcgatttc
    13381 tgtgcagatt tctttgactc ttgtacgcat aaagcattgg acatcttctg aaagagccct
    13441 aattgtacca atcatgatga cttcgtttgc gagaatgttg ctgcgcactc ctccctgaat
    13501 cttgccaatt gtaaggactg cattatcgag ggctgaaata gagcgagaga ctatggtttg
    13561 gagggcttga atgatagatg ctgaaactac aatagcatct acgccttgtt ccggataagc
    13621 agcatgggaa cttctgcctt tgacaatgat ctccaggctg tcggatgttc catagaaggg
    13681 accaatcttg acttctacca tgccagccat aagttcaggc acaacatgga ggcccagcac
    13741 cagatcgaca tgcggattct cgagacagcc agcctcaatc atggccttcg caccaccggt
    13801 agtctcttct gcgggttgaa aaagaaattt caccgagcct gagaaatccc gcctgtgagc
    13861 cgcaagtaat cttgctacac cgagcgctac ggtcatatgg gcatcatggc cgcaggcatg
    13921 catataacca tggtggagcg aggcataggg ccggtcagaa ggttcctcta ggggcagagc
    13981 atccatatcc gcacgcaagg caacgcagcg tcctggctct ttcccttcga taaggccaac
    14041 aacggctgtt ccatagcttc tatgaggtat atccatttct tcgagcatct tacagatgaa
    14101 actcgatgtc ttttgttcct tattccccgg ttctggaatc cggtgtatag acctgcgcat
    14161 ttctatgagc cattcttcca tggctctaga ttcgaaaaac acttcttttt gcagaaatgc
    14221 cagatttttc cattgagcca tatcaatccc ctttatcttt catggcaaga cgcattaggg
    14281 aaggcgaaaa taccagatcg aaagagtgct cggctggacc ggtcattttt atatttggct
    14341 cgcgcatatc gatatataaa gttccaccag ccattcttac tcttacctca ttctcgacaa
    14401 gaccgagtct tctagccgct gccgcagccg cacaggaaga cgatccgctt gccagggtat
    14461 aacccgcgcc acgttcccag atttctatcc tgatggtctg tctatcgatt acttccaaga
    14521 attgcacatt cgttcggtta ggaaaatcag gatgattttc aactaaaggc ccgatccggc
    14581 gcgcaagctc ttctgtggga tactggccta aaagtacaca atgtggatta cccatcgata
    14641 cccttatcgt gcgaaactca atgccatcga tagcaagcgt ggaactaccc ggtaagaatc
    14701 tagcttctcc catcgatact tcgatataat gctcttcttc atacccttct tctctatggt
    14761 catctttcct atagccatct tctccagaac atgggctatt tgtagctaga attcggcact
    14821 caaccatgcc acccttggtc tgcacttcaa aaggttcttt tcctacaaat ccgcgctcat
    14881 agagatataa ggaaaagatc cttaggccat tgcccgattt ttctgcctcc gagccatctg
    14941 gattgaatat acgcaacccg aatggactac agatcaccga ggaagtttgc gacagaatag
    15001 gcccatatag aattccgtca gagcccactc cgcggtttct atcgcatagg gcagagattg
    15061 cagaagctga aaacgacatc tgaaattccg ctggatcgat gacaagataa tcgttgccca
    15121 aggcctgata tttatgggca tgaaaaccct gttgtaagat tttcataatt attcctcttg
    15181 cggttttgaa tctatgtgta cgcggggcac tcgcttcgag atcatacaca ctacctcgta
    15241 atcgatcgtg gaaagcagat tcgaaatctc cgaaactgga agagtccgtc ccccaaaaag
    15301 caaaacctca tcgccaactc gcgctcccgc tatgccagtc agatctatca ttgtcatgtc
    15361 catacataca cgaccaacca caggcgcgcg aactccccga atcaataccg aggcacgatt
    15421 cgaaagtgct ctcggataac catctgcata accgattggc attaggccaa ttctgctgaa
    15481 gcgctttgta gtgaaagttc gaccgtaact aatcgattct tctgcgggta cttctctaat
    15541 atgtacgatg cctgtcgtaa gagccagggc aggctcgaaa ccttctggaa gctctatatt
    15601 cggcgcaggt cttagcccat agagcagaat accaggcctc accatatcga gtcttgtttt
    15661 tggatacaga accattccag cactgttcgc tatatggcga tacttcgggg taattccaac
    15721 ccgtccaaaa ctttcgagcg ctatggaaaa acgcttcatc tgaagatcgg tgaacgaagg
    15781 atcgggactg tccgcatctg cgaagtgcga gtatatgcct tccacgaaga tttccggata
    15841 gtcagcaagc agccccgcaa atgatctggc ttttgtccat gggaggcctt gacgattcat
    15901 gccagtatct atcttgatat gcgcttttgc ttttttcccc acgcttttag cggcggaagc
    15961 cagtatacgc acgtgctctt cggtatagat tgtctgagca cattcaaggc gtattgcaag
    16021 ggaggatgag gaatgctctg gcgcaccaaa gataaggaga ggcatttgaa tgccagactc
    16081 tctcagatcg agagcttctt ttgccagggc aacagaacaa aactgtgctc cttcgcgttc
    16141 cagctcatgc gctattgcca gagccccgtg tccataagca tcggctttga tgacggggca
    16201 gacaatgcta ccctgagcta tatctctaag gtggcgaaag tttgctcgga tggcggcaag
    16261 atcgatccat gccgtcgttg cacgatgatc catgactcaa tccttctttt gtaccctatg
    16321 taccttaaat ttactactga gcaaatcgtt tggcttatag tcattttctg aagccagaag
    16381 cgctttgggt cttacggcag tctccaaacc cttgtcccag ataaaaatat cggctatttt
    16441 cccaacttct atcgaaccat attctccatc catccccagc gccttgcgcg gggttagcga
    16501 catcgctcgg actgcgcgcc agagtggaac ttctgccttt aaataatagt tctttacgat
    16561 atcaattccc agcatattgg agcccatgag gacacccgaa tctgcatacc tcacacctcg
    16621 ctcagaagag acaactctcc tcccataata gctgaattcc ccatacggaa gcccagctcc
    16681 tatgacggca tccgaaatca gtattagcga attctcagga atcaagcgag agcagagctt
    16741 tagacaactc tcgttcacat ggattccatc gccatttagt tcaacatagt ctggagattg
    16801 tgaaaaagcg aaattagcga gtcccccttc tctgtgatca atccctgtca tcgcattaaa
    16861 tagatgagtg atggaaaaag gtcgagacgg aggatggatc gaagataatt tcgcatctga
    16921 atgaccaagt gaaacaagaa cggctttatt ctggaaaact gggtatatct ggtcgatccc
    16981 gtctagctcg ggagccattg tacatatctt cagcaaccct ttggatatgt cgagaatatg
    17041 ctctgcgagc gcgatgtcag gtgctcgaat attatctggc ataataccac cgcgcttcga
    17101 atgatttaca aatggacctt cgagatatat cccaggaagc agaggcgcag aaatcccgct
    17161 cgccaagatt gtctctgtaa gctcaatgag ggttctttcc tcccagagaa tcgtcggtac
    17221 atagcatcct actccttttg cctcaagaag agctgctatt tgaaaaagat cccctgcctc
    17281 tgaaattctc tcgaaccccc aagatgcaca gccatggatg tgcatctccg ctagggaagg
    17341 aagcacaaaa gcatcttcgg ccttgattac gggagcattt tcaaatgctg ggtcaatctg
    17401 agattgagaa gatcgattga gctcttccgg ccgctggaat tgcccggaag ggattattgc
    17461 agctattttt cctcctcgca ctgccagcgt tgcctttggc aatatcgaat ccggaagtac
    17521 aaggtctgcg ccttcgagaa tataggtgtc atcataatgg ttgtatgtca tgaaagaaca
    17581 ttgtgccgct tctatgtcgg catgtaaata atactgctac gaaccttgtg tatnnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccttgct cttcccttat tcgggatatt
    17761 tcttacatct tacttcaact tcttatacac aaggttcgta gcagtaatga ccaattacca
    17821 actttcatat attatcacca catgacagcc tcgaaaaacc tagacccatc taaactcaga
    17881 ttcattgcaa tggatatgga tggaaccatt ctcgatgagg gatatcagct ttctccccat
    17941 gtggctgata cactcaaaaa gctccaaaac caggggaaaa aattgatcat tgcaaccggg
    18001 cgcatctata aggccgcaaa gattgtcctt gcggattcct ttgaaccaga tgccttcgta
    18061 tgcacgaatg gagccgatat ctataaccgc gacggtacga tcttggcttc acatcatatt
    18121 ccgaccgagg ctctgccgat actcacaaaa gtaagcagag accatgatgt gcttttctgt
    18181 tgctatgttg gagaacgatg gatctacgat cgctattcgc caggtgtgga tttttacgaa
    18241 aagcgaactg gcatgaaagg tgttcggaag aatttcgact ctctcgttgg cgaagatatt
    18301 ttgaaatttc ttgcagttgg cccaaatgag aaacttctcg agatgcggga aacgatatta
    18361 aactcagcac ccgaccttct tgatgcgttt ttttctcacg agacaatgct ggaaatcgtt
    18421 gcaagcggcg tttcaaaacg gcaaggtctt gaggaatgtc tacattattt gggcggtagc
    18481 cttgaagaga caatcgcatt tggcgatgct gaaaatgatc tggaaatgct aaaatccgtt
    18541 aaggttggtg ttgctatggg aaatgcccat agctatattc aaacagcagt gccttatgta
    18601 accgcctctg tggatgatga tggagtggcg cgttttctgg aagagctttt cggaatatga
    18661 tttgatctgt tatacaagat cggctataca agccataaaa aggcattaat cacaacatag
    18721 ccttgagtga cttcgatact tcgattgtct tcttgactgc tttttatgtg ctggctaaaa
    18781 tctagattcg aattgccggg gtggtggaat tggtagacac cagggactta aaatcccttg
    18841 ctgcgcaagc ggcgtgcggg ttcgat
//
LOCUS       DAOOWY010000031        18227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3074_length_18227_cov_11.635648, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000031 DAOOWY010000000
VERSION     DAOOWY010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 18227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18227
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_18227_cov_11.635648"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1131)
                     /locus_tag="PK105_03925"
     CDS             complement(28..1131)
                     /locus_tag="PK105_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60888.1"
                     /translation="MHPFSFCPNPACPHHQVAPEGSWYVALGFYYTKCFGDVPRYRCK
                     TCGRTFSSQTFSLDYFAKKRLDYRQLERLVSSSMSQRALSRHFKVSLGTINNRIQRLS
                     HQSLATHTLLRPQAAPREPVCIDGFVSFDRSQYFPNNITISLTAHSQFILSLTHATLR
                     RSGRCTDTQKHRKNLLYRSVTFEKAALQRSFSELLDQLYSERCTALTSPSLLITDEKP
                     DYAWALTRHISRFSLHHLTVNSKAPRTLHNPLFASNYIDRELRKDLADHRRETVCFPR
                     NVANMLNRLVVYLGWHNYEKPFRIGQSIESTHAEHAGIKKSAIYKARLTQFKQRAFLT
                     RTHLFPHERKLWLRGFPTPLKNKPEYVPAYAYA"
     gene            complement(1212..1865)
                     /locus_tag="PK105_03930"
     CDS             complement(1212..1865)
                     /locus_tag="PK105_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HOU60889.1"
                     /translation="MNKNQKESSSDSSKPGLPHINIRFQSPIDELRIELTRKSLHLLI
                     LFCIPLLDISPMLPVLALIAGMLLYSVSEILRHRGFNVPIISSITERAARRRDSNRFV
                     WGPITLALGATFSLLIFDPLQAKVAILALGLGDSFSSLVGKSFGRIPMPFSGGKSVEG
                     SILCFAIVLLAVYLLTGRFIPSMIIALATTFAEFWPTKDWDNILIPVAAGIIASLVL"
     gene            1940..2011
                     /locus_tag="PK105_03935"
     tRNA            1940..2011
                     /locus_tag="PK105_03935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1972..1974,aa:Val,seq:cac)
     gene            complement(2130..3365)
                     /locus_tag="PK105_03940"
     CDS             complement(2130..3365)
                     /locus_tag="PK105_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU60890.1"
                     /translation="MNALKRYFDVYRGLSQSLYILFFATIINSMGMFVFPFLTLYLTG
                     RLAMTQEKAGFFIMMLNLVYGPASLVGGKLADSFGRKRLMVFAQVLSGLCYIPCGFPS
                     VGSNIRWFLLASVFFDGLTDPARSAMMTDLTSPENRRPAFSLIYLGHNLGFAVGMLLA
                     GFLFENYTSWLFWGNAIAIFVAITLVGLKVTETKPTRGQIEKSFGTGRQDEAHRGNII
                     QALLSRPNLIIFTMLTGLYGFVYAQHRFILPLQTKEFFGASGSVIYGTLMTLNAAMVI
                     VLSTPIMTITQHWKPINAVALSGLLYALGFGFIGLRPSIMLIYITTAIWTLGEIVLAT
                     NEGTYVANSTPISHRARFQAFLPLLGGIGLTISPPIVGSFTDHFGLKMAWPLLGAIAA
                     VASLSIYYLGVMEEKANKK"
     gene            3590..4435
                     /locus_tag="PK105_03945"
     CDS             3590..4435
                     /locus_tag="PK105_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU60891.1"
                     /translation="MKGIIVAAGYGTRFLPATKTVPKELLPIGITPSIAFIVKEFVDS
                     GIQDIIIISSRRKKALEDYFDREIELEALFEREARQDQLRKILPFDAHITFVRQMEMR
                     GTGHALMQCANLLDGEFSIVAYPDDIVLGPKPLARQLIDMYEETGKCVLATILETGDV
                     SRYGVIDPAPDGVGVRGFIEKPEKGKEPSHEVSIGRYLYTPRFFEWLEEGWKHHGSGE
                     YYHTYALQKMISEGKVAFSRVQGQRLDTGEPAGYFEAQLHNALNDPSLKKVLAHFVEE
                     HKNEL"
     gene            complement(4604..7021)
                     /locus_tag="PK105_03950"
     CDS             complement(4604..7021)
                     /locus_tag="PK105_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOU60892.1"
                     /translation="MCGIVSLVYRDENPSIGKEAEALLKRLEYRGYDSTGAAFIGADK
                     RILLRKKVGAPSRVCPLLQIDSFSGQRFIGQVRWATYGSVTDVNSQPHHVHCKMELVG
                     AHNGNISNTDTLKTWLSARGHAVISDNDGEIIVHLIEEHYSAAQSLASSDLAYLRKAY
                     ANAGLAEGVPDGVLRMIEAIRRAQALAQGSYAAAIADPQLPGVFAIKSGSSLYAGVGS
                     DSFGNFIVLSSDLTSVLSKTRTLIPLSEGEGIWFTDKEYLVFNLQGNPFFSHPRLKRS
                     KLSIKDTQLSPEYQHYMEQEISSSMNNINQILRYYFRDSSTEPLSELFEDHKDDCKEA
                     AEKISSLSERFGNEALVSGMKEIFESPAWNEIEARVQNTGAAHFVGQDFISDETELLK
                     ELAALNPPMRGKLLLMDKVIVWRKRRAVLRYTGELKNAIRETTQSGGRIYFIASGTSY
                     HAALIAAYFFNSTAHIPVYPCNPGMFRSMYFNCMGKNDLIIGISQSGETKDLVDIFQD
                     VKKTNNSVRHVSIVNNENSRIPQELSDFYLPLLCGPEIAVAATKSFTSQIAILYLLAA
                     SFSMPEHKVRENLIKARDLMTETIETRMGDIESIAAHLFMASSIHILGTGLIGLAKEG
                     ALKIREIVLNHTEGYDAAEFKHGPNTILGRNTLLSLADLEKLVGGEPLTAITNDLFEK
                     LSHNYPLIFICPPEERDRRITISQIHTHKIRGADIILIAEPSQELALAVEGVPMGIEK
                     YWSKYLPLPQSGDPCIFVFSAIIALQYLAYRMSVRKMQWLDSLGIQNHGVHPDAPKNV
                     SKSITVD"
     gene            7250..8143
                     /locus_tag="PK105_03955"
     CDS             7250..8143
                     /locus_tag="PK105_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOU60893.1"
                     /translation="MIKPLEIHYEVRGYDCGYGGPLKPFALANFFQETAGVHATVLGI
                     GMEDMWARGLTWMLSRIDIRIEAMPRTGQRVIARTWPVGAKKIYAQRCLELLDESGFR
                     YAGALYEYIIVDMKTRRVVRPERTLPIDLTTDYPLPFVDLSPGVEDASFGAFPQATNH
                     SMQDKKDPYAEPKNTLLLNGFSEVFALEARTRHIDQNGHVNNAHFINWLCDAVPCADG
                     ETICRIKVDFVREILKGGKISVWIKPIHTISNSVSENALKQAEKIKAPHSSWLSALAC
                     DGILAAQAMITATNLVYNKLK"
     assembly_gap    8458..8557
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8558..9079)
                     /gene="tpx"
                     /locus_tag="PK105_03960"
     CDS             complement(<8558..9079)
                     /gene="tpx"
                     /locus_tag="PK105_03960"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOU60894.1"
                     /translation="MANITFKGNPIQTIGSLPAKGATASDFRLTKSDLSDVSLEDFEG
                     KIKILNIVPSLDTGVCAASARAFNKAASSIDDVVILTISRDLPFAQKRFCEAEGIDKV
                     ITLSELRTRNFGKAYGVEIISGPMAGLLSRAVVVLDKANKVIYTEQVPEIAQEPNYKA
                     ALEAARTATNLVYK"
     gene            complement(9161..9901)
                     /locus_tag="PK105_03965"
     CDS             complement(9161..9901)
                     /locus_tag="PK105_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU60895.1"
                     /translation="MANMLFSIVLAFSLSAMTGYAQGWPEGKESDFFDTLKGKLEIVF
                     LGHGSLLLAYNGSNIYIDPVSQYANFSKYPKADLILITHEHNDHLDPKAIAALSKPET
                     RIILSEASRKKLGHGEVLQHGHSIDVAGVTVLAVPAYNITSSRMGYHPRERKDNGYVL
                     SIGSLRIYIAGDTEPIPEMANIGKIDIAFLPMNLPFTMTPTQVAQAARIIKPRILYPY
                     HFGSTDTSEITRLLSSDKDIEVRIRNLK"
     assembly_gap    10278..10377
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10639..11286)
                     /locus_tag="PK105_03970"
     CDS             complement(10639..11286)
                     /locus_tag="PK105_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU60896.1"
                     /translation="MSRRYVVVAGNIGAGKSTLVSLLAEHLGFIAYLEPVLENPFLKD
                     FYANMSRWAFHSQLFFLSHRADMHRKLSEDPHSVVQDRSIYEDAEIFARNLYIQGIMA
                     EREWCIYKALYATLAEILPHPSLVIYIRASVSTLQSRIKQRGRDFESSIPDSYLEGLN
                     NLYEEWISSFTLAPILTIPGDSLDFVTESRDLQVILRTITKRLEDNQPYLFPYEM"
     gene            11484..12836
                     /locus_tag="PK105_03975"
     CDS             11484..12836
                     /locus_tag="PK105_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60897.1"
                     /translation="MKRFVILLFAWLAVIASIGAQQAPAVQSPYSDAAPQTKEIIDAA
                     RLLEAKGQYSKAWNLLAAFDPDSKDGYVLAEKIRIALEDSIGNGMLVSFSFVDLAEGQ
                     SIYEAFLNPPEKQTIVDFNPLDLVEALEKSSEAIPPILSLELAGYLYAVNQQYSDNWM
                     IDGYTIQQKAVENYDRALAYGLYNDKSLANHAELLMNLQQYEGAEKVLRQAISLFPEE
                     LSFAVSLANSLNYQGKFDQVYSVIDKVIEDPDVTSSTYNAYVEGIKAGLNSGDTQKTD
                     SYVDAMIERFPDEYAPMLIQHLVAVIMGEIEEANVSADSVTEKFNVDPNVVQSLLSTW
                     LGASDPVSGFDYLNRSLEKYTSDDQAMGTLFFYRALMYAQTARSADDLKLAIVDMEET
                     EKRFSAVYDKEDPVFQTIEQLLTEWKQIIEQLQESDSASSSEQSTPPEDSNTASGTSN
                     "
     gene            12860..14056
                     /gene="nagA"
                     /locus_tag="PK105_03980"
     CDS             12860..14056
                     /gene="nagA"
                     /locus_tag="PK105_03980"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOU60898.1"
                     /translation="MQSVFCIHNATVIAGYARMDRSAVLVEGDSIADVFSERRFAQKS
                     FGPQVHLIDAGGAYLTPGFIDTHIHGIGGFGTEDMSAEAILAMSEILPAWGVSSFVPT
                     LYPMPEEDMIRAIRAVVLAMGHETGAEIIGIHIEGPFISPAQLGVQRIEFVRPVDLEL
                     MQRLWDASEGHIVSMTVAPELKGMRELALFCNQLGIVLQAGHTDASYENMIEGMQAGI
                     RHSTHMFNAMSRLHHRNPNAVGAVLIQSDLSCEIIADGLHVHPDLIRLLFRDKPEGNI
                     VLVTDSLKPTKQAANAPMFANDEEVYLEDNLFYRARDGVIAGSSLTMIEGVLNLVRFG
                     VPLESAVKMASANPARIFGLRRRGMISPGYKADLVLANADFDVQMTIIDGKIKYDHNA
                     IERRII"
     gene            14053..14856
                     /gene="nagB"
                     /locus_tag="PK105_03985"
     CDS             14053..14856
                     /gene="nagB"
                     /locus_tag="PK105_03985"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOU60899.1"
                     /translation="MRVVIQQSYELMSKWAAYYLVQHINEHDSQKPFVLGLPTGSTPL
                     GMYRELITLCKAKKVSFKNVITFNMDEYVGLTSEHEQSYHRFMWDNFFSHIDIEPKNV
                     HILNGMAIDLQKECTAYEKAICDAGGIDLFVGGLGADGHIAFNEPGSSLSSRTRIKTL
                     TRDTKKANARFFGGDPEKVPSQALTVGVGTIMDAQEVMILVSGMEKARALKHGIEMGV
                     NHMWTISCIQLHPKAIVVCDEDATNELRVVTVRYFKEIEAPNLRNWDLD"
     gene            14901..15767
                     /locus_tag="PK105_03990"
     CDS             14901..15767
                     /locus_tag="PK105_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU60900.1"
                     /translation="MRSDIKGIMAMVATSVCWSLSGLFIKLIDWHPIAIAGARSLVAA
                     LFIFAVVRKPKLNFGKNQIIAAISYSCTMLLFVYANKNTTSANAILLQYGAPVYVMLL
                     SGVMLKEKPLPEQIGALIAIVIGMCLFFADSLSLGHIKGDVAAVLAGMTFAIHMLFMR
                     QQKEGSPIESLFISHCMTAIVSLLICIFLPPPVISKRSVAAILGLGIVQIGFSAVFFS
                     YAIKRITAIQSSLISIIEPALNPVWVYLAIGEKPSRMAILGGVIILSAVVISSIFSVS
                     RSARPSSMDGGT"
     gene            15905..16513
                     /locus_tag="PK105_03995"
     CDS             15905..16513
                     /locus_tag="PK105_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOU60901.1"
                     /translation="MRQSFLSVTILLILITDPLGNIPLFIASLKHVPKGRRSEVILRE
                     CLIAFLVLVGFLFFGEVFLSALNLSDDILRISGGVILFLIALNMIFPGTGGKLVEDEI
                     EGEPFIVPVAIPLIAGPSAITYVMLLMKSDTSRALEWVGAIFIAILVTMIAFFMSNKL
                     KEWLGPRALSAIERLMGLVLTTIAIEMLLSGLLQYLSSVQNL"
     gene            complement(16606..16878)
                     /gene="yccX"
                     /locus_tag="PK105_04000"
     CDS             complement(16606..16878)
                     /gene="yccX"
                     /locus_tag="PK105_04000"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOU60902.1"
                     /translation="MKAFHALIKGEVQGVGFRMSAVIRAERLGLKGWVRNTEDGDVEV
                     WAEGDPQALEQFYSWLQVGPAAARVDRVLKTDEIPKGNYIRFSVSF"
     gene            complement(16909..18117)
                     /locus_tag="PK105_04005"
     CDS             complement(16909..18117)
                     /locus_tag="PK105_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU60903.1"
                     /translation="MIVEKRLLSLYICLFAVFALFGISMTVIGATLPKIFAEFGWSYM
                     TAGMVIAAGSVGYFLSSYLAGILFPFLGLRFLISLSLILICCGLEFFAKTPTAWLNTM
                     LYFIIGVGQGGIEIAVDWAVLRMEGPGGGKAMSLMHGAFSIGAVVGPLVIAILISANL
                     EWTLVYRIMGVLFLGIAIFMQFMPFKLLGKGRGQITNRSRRDLFHQPMYWLGFFALFV
                     YVGVELGISNWIAEYFVSVFGAPIPTGSFMVSLFWAGLLMGRFGIPIIQKSIKRERIL
                     IALSTLMAISLIAFNMIGFIGDGKLASVIAGILVILAGFGCSIIYPTIMSIIGDYFLH
                     AQSEAVGFAAMGGGIGGFLFPYMMSAVSTGWGIRIGFAIYALFSLLMVVLNIFLVRAQ
                     EQKSIGVSEP"
BASE COUNT         5072 a         3944 c         4286 g         4725 t
ORIGIN      
        1 actttaggca ttctataagg aattgtctca agcataggca taggctggca catactctgg
       61 tttattttta aggggagtgg ggaagccccg gagccagagt tttctttcat gagggaacaa
      121 gtgtgttcga gtaaggaatg cgcgttgctt gaactgtgtt agccttgcct tataaattgc
      181 gctctttttg atacctgcat gttctgcatg tgtgctctct atggattgtc ctattcgaaa
      241 aggtttttca tagttatgcc agcctaagta tacgacaagg cgattgagca tattagcgac
      301 atttctgggg aagcagactg tttcacggcg atggtctgcg aggtccttac ggagctcgcg
      361 atctatatag ttggaagcaa acagtgggtt atgtagggta cgaggagctt tagaattgac
      421 ggtgagatgg tggagagaga aacggctaat atggcgagtg agtgcccatg catagtcagg
      481 tttttcgtcg gtgatgagca atgatggaga agtgagagca gtacagcgtt cgctatatag
      541 ctggtcgagg agctctgaga aagagcgttg gagggcagcc ttttcgaagg tgactgagcg
      601 atagagcagg ttttttctat gcttttgagt gtcagtgcaa cggccagagc gacgtaatgt
      661 ggcatgggta aggctgagga tgaactggct atgggcggtg agtgagatag taatattatt
      721 ggggaagtac tgggagcgat caaagctgac aaagccatca atacagacag gttctctggg
      781 tgcagcctgt gggcgtaaga gggtatgcgt ggcaaggctt tggtggctta atcgctggat
      841 gcggttattg atagttccca aagagacctt gaagtgtctg gagagtgcgc gctgactcat
      901 gcttgaagac acaagacgct caagttggcg atagtcgagc cttttcttgg caaagtaatc
      961 taaactgaat gtctgtgaag agaatgtgcg gccgcaggtt ttgcagcgat aacgaggtac
     1021 atcgccaaaa cacttggtgt aataaaagcc gagcgcgaca taccagctac cctcgggagc
     1081 tacttggtga tgaggacagg cgggattagg acagaaagaa aaaggatgca tggattacct
     1141 ccgtacatcc tctttatcgc cctcatctct tcatcttgct tcaacttctt atacaaggtt
     1201 cgtagcacta atcaaagtac cagagaagca attattcccg cagcaaccgg aataagaata
     1261 ttgtcccagt cctttgtagg ccaaaattcc gcaaaggtcg ttgccaatgc aatgatcata
     1321 gatggtataa agcgacctgt aagcagataa acggcaagca agactatggc gaaacacaat
     1381 atcgatcctt ctaccgactt tcctccggaa aacggcatcg gaatgcgacc gaaagactta
     1441 cccaccaggc tcgagaagct gtcgccaagg ccgagcgcga gtatagccac tttggcttga
     1501 agaggatcaa aaatgagcag agagaaagtc gctcccagcg ccagtgttat tgggccccat
     1561 acgaagcgat tgctatccct gcggcgtgcg gctctttctg tgatcgacga gattataggt
     1621 acattgaagc cccggtgtct tagaatctca cttacggaat agaggagcat tccggctatc
     1681 aatgctaaga ctggtagcat gggcgaaata tcgagaagag gtatacaaaa caaaatgagg
     1741 agatggagag attttcgcgt gagttctatg cgcagttcat cgattggaga ttgaaatcga
     1801 atattgatat gagggagccc aggtttagaa gaatcagaag aggattcttt ctggtttttg
     1861 ttcactttag ctcgcttcct cgactatcct tgacatattg cggcatgctt tgttaggatg
     1921 ccgcagaaga gtgttgattg ggcgattaac tcagcgggag agtgccacct tcacacggtg
     1981 gaagtcattg gttcgaatcc aatatcgccc atacagcact ctttttgtat cggcatgaat
     2041 tgcacaagag aaaatcgtta attctatgaa gttatgatat acagaaatca atcattgcaa
     2101 gcgcttacca agggctcatg tttgaacgat tatttcttat tggctttttc ttccatcact
     2161 cccagataat aaatagacaa actcgcaaca gccgctatag ctccaaggag gggccaggcc
     2221 atctttagtc caaaatggtc ggtaaaagaa ccaacgatgg gtgggctaat ggtcaagccg
     2281 attccgccta gaagtggaag aaatgcttga aatcttgccc tgtgcgaaat tggagtacta
     2341 tttgcgacat aggtaccctc gtttgtcgca aggacaattt cgcccaaagt ccagattgcc
     2401 gtggttatgt agataagcat tatggaaggt ctaaggccaa taaatccgaa tccaagagca
     2461 tacaataagc cggagagggc taccgcattt atgggcttcc aatgctgggt gattgtcatg
     2521 attggcgtgg atagcacgat taccatcgct gcattgagtg tcatcaaagt gccatagata
     2581 actgaaccgc tagcaccaaa gaattccttg gtctgaagtg gcaagataaa tcgatgttgt
     2641 gcatagacaa agccatagag ccccgtcagc atagtgaata tgattagatt aggccttgaa
     2701 agaagggcct ggataatatt gcctctatga gcttcatctt gcctgcctgt accaaagctc
     2761 ttttcgatct gcccgcgagt aggctttgtc tccgtaacct ttagtccgac cagtgttatg
     2821 gctacgaata ttgcgattgc atttccccag aaaagccagg atgtgtagtt ttcaaataga
     2881 aagccagcaa ggagcattcc tactgcaaag cccagattgt gacctagata aatcagagaa
     2941 aaagccgggc gtctgttttc gggggaagtg agatcagtca tcattgctga gcgggctgga
     3001 tcagtcaacc cgtcaaagaa aacacttgca agcaaaaacc agcgaatatt gctaccaacg
     3061 gatgggaaac cacatggaat atagcacaag cccgacaaga cctgtgcaaa taccatcaat
     3121 ctttttcgcc caaagctatc ggcaagcttt ccaccgacaa gagaagcagg accatagacc
     3181 aggttgagca tcataatgaa aaaaccagcc ttctcttgag tcattgcaag ccgtccagtc
     3241 agatacaaag taagaaaagg aaacacaaac atgcccatgg aatttattat tgtagcaaag
     3301 aagagaatat agagagattg cgagagccct cgataaacat cgaaatagcg ctttaatgca
     3361 ttcataaata gcaaacatta catggcttaa gatgaagagg tcaagggcag cctattatga
     3421 gcctcctgaa ttgcctcgaa aggcttaagc gtagaacaag agttttctgc aaatgccaca
     3481 gcagcgatgc ccaatggttt attttttatc tggattacat tcaagaataa ggacaattcc
     3541 gattcagata gaattgtcat gatttcaatc atattttaca atagctctca tgaaaggaat
     3601 aatcgttgca gcaggctatg gtacacgttt tctacccgca accaaaaccg ttcccaaaga
     3661 acttctgcct attggtataa caccgagtat tgcctttatt gtcaaggaat ttgtagattc
     3721 cgggattcag gatataatca taatctcgag cagacgcaag aaagctctcg aagactattt
     3781 cgatcgagaa atcgaactag aggcgctatt tgaacgagaa gctcgtcaag atcaattgag
     3841 aaagattctt ccttttgatg cccacataac gtttgtccgt cagatggaaa tgaggggcac
     3901 aggccatgct ctgatgcaat gtgctaatct tctcgatggg gagttttcca ttgtagccta
     3961 tcccgatgat attgtgctgg ggccaaagcc cctagctcgc cagctcatcg atatgtacga
     4021 agagacaggg aaatgcgttc ttgccacgat attggagact ggcgatgtgt cgcgctatgg
     4081 tgtaatagac ccagctccgg atggtgtggg agttcgaggc tttatagaaa aacctgaaaa
     4141 aggcaaagag ccaagccatg aagtctcgat tgggcgttac ctctatacac cgagattctt
     4201 cgaatggctc gaggagggtt ggaagcacca tggttcaggt gagtactacc atacatatgc
     4261 gcttcaaaag atgatcagcg agggcaaggt cgctttttcg cgagtccaag gtcaaagact
     4321 agacaccggt gaaccagctg gatatttcga ggctcagctt cataacgccc ttaatgaccc
     4381 ttctctcaag aaagtactag cccattttgt agaggaacat aagaatgagc tttgatcatc
     4441 ccctcataac aagcctgttc ctgtccgtgc acataatgcc tgctactgtc tatcaaggcc
     4501 taatgcttgc ttgcgcttat taaaaccgcc ctctgtgctt attgggagtc gaattctagc
     4561 aaaatttttc cgaccaactg tctcggccta tagccacttt cgattagtcc actgtaatcg
     4621 acttggagac attcttaggt gcgtcgggat gtacgccgtg attttggatt cccagtgaat
     4681 caagccattg cattttgcgc accgacatac ggtaagcaag atattgcagc gcgattatag
     4741 ccgagaatac aaagatgcag ggatcgccag attgaggcag gggaagatac ttagaccaat
     4801 atttctcgat gcccatcggc acaccttcca ctgcgagagc cagctcttgc gaaggctctg
     4861 cgatgagaat aatatccgct cctctgattt tgtgagtgtg tatctggctt attgtgatac
     4921 gcctatcgcg ctcttctggc ggacagatga atatgagcgg atagttatgc gaaagctttt
     4981 cgaagagatc atttgtaatg gcagtcaatg gctcgcctcc gacaagcttt tccaggtctg
     5041 ctagagaaag caatgtgttc ctgccaagga tagtattggg gccatgcttg aactctgcgg
     5101 catcatagcc ttccgtgtga tttaggacta tttcgcgtat ctttaaagcg ccttcttttg
     5161 ctagcccgat gagtcctgtt cccaggatat gaatagaaga agccataaag aggtgtgcgg
     5221 ctatactctc gatatcgccc atcctggtct cgatagtctc ggtcatcagg tctctggcct
     5281 tgatcaggtt ctctcgaact ttatgctctg gcatagagaa gcttgcggct aaaaggtaga
     5341 ggatggcaat ctggctcgta aaggatttcg ttgctgctac agctatctcc ggtccacaca
     5401 agagcggcag atagaaatcc gaaagctctt gaggtattcg gctattctca ttattcacta
     5461 tgctgacatg acgcaccgaa ttgttggttt tctttacatc ttgaaatatg tcgactagat
     5521 cttttgtttc gcccgactga gatattccga taatgagatc gtttttcccc atacaattga
     5581 aatacattga gcgaaacatg ccaggattgc agggatagac cgggatatgt gctgttgaat
     5641 tgaaaaaata agcggcaata agagctgcat gatacgatgt gccggatgcg atgaagtaaa
     5701 ttctgcctcc tgactgtgtg gtttcgcgga tagcattctt gagctcaccc gtatatcgca
     5761 gaaccgcgcg gcgctttcgc catacaatta ccttgtccat gagcagaagc ttccctcgca
     5821 taggcggatt gagggcagca agctctttga gcagctctgt ctcatccgag ataaaatctt
     5881 gaccgacaaa gtgcgctgca cctgtattct gaactcttgc ttctatttcg ttccaggctg
     5941 gagattcaaa aatctccttc attcctgaaa ccagagcctc atttccaaaa cgttcagaga
     6001 gcgaagaaat tttctccgcg gcttccttgc aatcatcctt atggtcttca aaaagctctg
     6061 aaagaggttc ggtagaggaa tctcggaaat aataacgtag aatctggttg atgttgttca
     6121 tgctcgatga gatttcctgc tccatatagt gctggtattc gggtgagagc tgggtatctt
     6181 tgatcgagag ctttgatctc ttgaggcgag gatgtgagaa aaaggggttg ccctgaaggt
     6241 tgaatacgag gtattcctta tcggtaaacc aaattccctc tccttcggaa agagggataa
     6301 gcgtgcgcgt cttagaaagc acacttgtta gatccgaaga aagaacgatg aaattgccaa
     6361 aagagtcact gccaacgccg gcatagagcg aagagccaga cttaatggcg aatactcccg
     6421 gcagctgagg atcagcgatg gctgcggcat aggaaccttg ggcaagcgct tgagctctgc
     6481 gaattgcctc gatcatgcgc agaactccat cgggcacgcc ttctgcaaga ccggcattcg
     6541 cataagcctt tctaagatag gcaaggtcag agctagccag cgattgggcg gcagaatagt
     6601 gctcttcaat gagatgcaca attatctctc catcgttgtc cgaaattacc gcatggccgc
     6661 gggcggaaag ccaggttttg agcgtatcgg tattcgagat attgccatta tgggctccaa
     6721 ctagctccat tttgcaatgc acatgatgag gctgactatt gacatcggta acgcttccat
     6781 atgtagccca gcgcacctgt ccaatgaatc gctgaccact aaaggagtcg atctgcagga
     6841 ggggacagac tctggaagga gcaccaactt tttttcgcag caagatgcgt ttatcagcac
     6901 ctataaaggc agcaccagtc gaatcgtaac ctcgatactc gagacgcttg agaagcgcct
     6961 ctgcttcttt tcctattgaa gggttttcat ctctatagac aagtgaaaca ataccgcaca
     7021 tagctttgta ttataccatg atattccatg gctgcggagt caatttgtcg gagttcatta
     7081 ttagagccca tctatcatag catattcata agaagcaaaa ggcttcggaa tctccataat
     7141 gcctttctta gaaacatgca acgaggtagt atatgaaatg tcagaaggat atcaacgcta
     7201 tttggtgatt agtgaataaa gaagaccagg aaaggatcag gatgcatcga tgataaaacc
     7261 tcttgaaatt cattatgaag tgagaggata tgactgcggt tatggcggac ctcttaagcc
     7321 ctttgcactt gccaatttct ttcaggagac agcaggagtc catgcaacgg ttttgggcat
     7381 cggcatggag gatatgtggg ctagagggct tacgtggatg ctttcgcgca tcgacatccg
     7441 tatcgaagca atgcctagaa ccgggcaaag ggttatcgcc agaacttggc ctgtcggagc
     7501 aaaaaaaatc tatgcacaga ggtgtctcga acttctggat gaatcgggct ttagatatgc
     7561 aggtgccctg tacgaatata tcatcgtgga catgaagact cggcgtgtgg ttcgaccgga
     7621 gagaacgctt ccaatcgatc tgaccacgga ttaccctcta ccatttgtgg atctctctcc
     7681 tggcgtcgag gatgcttctt ttggagcatt tcctcaagcg actaaccact caatgcaaga
     7741 taaaaaagat ccatatgcgg aacctaagaa tactctcctg ctaaatggtt tttccgaggt
     7801 ctttgcttta gaggctcgca cacggcacat cgatcaaaat ggccatgtca ataatgctca
     7861 cttcataaat tggctatgcg atgcggtgcc ttgcgcagat ggagagacta tctgtcgaat
     7921 caaagtcgat tttgtacgcg agattctaaa aggaggaaaa ataagcgtat ggataaagcc
     7981 cattcatact ataagcaact ctgtatccga aaatgcattg aagcaggcag aaaagattaa
     8041 agccccacac tcctcttggc tttcagcact tgcttgcgat gggattcttg cagcacaggc
     8101 tatgattact gctacgaacc ttgtgtataa taagttgaag taagaagcag gaaatagagc
     8161 caataaggga agagcaagga gctaactatg tagactttcc cttctcgata gagagcttag
     8221 aagagagctt agaaagccca ttgcggatca tcgcagagaa tcggtgtacc ttgcaaggaa
     8281 tgtgtccagt gtgctgcata gacttgtgct gtaaatgctg tatatataag aagaaagcag
     8341 atcatgtacc tgtctatgct tatgcatatg cataagaagc cttccttatg ccattctcaa
     8401 tccttataca caaggttcgt agcagtacga gcggcctcca aagcagcttt atagtttnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt atacacaagg ttcgtagcag
     8581 tacgagcggc ctccaaagca gctttatagt ttggctcctg agcaatttct ggcacttgtt
     8641 ccgtgtatat aaccttattg gctttgtcga gcacaactac cgctcgcgat aaaaggccag
     8701 ccataggacc agagataatc tcgacaccat aagccttgcc aaaatttctt gttcgcagtt
     8761 cggatagggt gataacctta tctattcctt cggcttcgca aaagcgcttc tgtgcgaaag
     8821 gcaggtccct actgattgtc aaaatgacta catcatcaat agaagatgca gccttgttga
     8881 atgctcgggc actggcggca cagacgccgg tgtcaagact aggaactata ttgaggattt
     8941 tgatcttccc ttcaaaatcc tctaggctta catcgctgag gtcagatttg gtcaatctaa
     9001 aatccgaggc cgtggctcct ttggcaggaa gagaccctat tgtttggatt ggatttcctt
     9061 taaacgtgat atttgccatt cttatactcc tatagatagc tatatgaaat aaatatatcg
     9121 aaaatcatat attatcggca aggtaagaat agctgctctg ttatttaaga tttcggattc
     9181 ttacttcgat atctttatcg ctggagagca atcttgttat ctcgcttgta tcggtcgaac
     9241 cgaaatggta aggatagaga atcctgggct tgatgattcg ggcagcttga gcaacctgcg
     9301 ttggtgtcat ggtaaagggc agattcattg gcaaaaatgc aatatcaatt ttaccgatat
     9361 ttgccatttc gggtatcggc tccgtatctc ctgctatgta gatacgcagt gaaccaatac
     9421 tcagaacata accattgtct tttctttctc ttggatgata gcccatccgg gacgaagtga
     9481 tgttataggc aggtactgcg agcacggtta ctcctgcaac atcgatcgag tggccatgct
     9541 gtagaacttc cccatgacca agctttttgc gggatgcctc cgacagaatt atgcgtgttt
     9601 caggcttgct gagagcagct atagcttttg gatcgagatg gtcgttgtgc tcgtgtgtta
     9661 ttaagattaa gtcggccttg gggtacttgg agaaattagc atattgactc accggatcta
     9721 tatagatatt agagccgttg taggctagaa gcaaagagcc atggccaaga aagactatct
     9781 ccaatttgcc tttcagtgta tcgaagaaat cggattcttt tccttctggc cagccctgag
     9841 cataacctgt catcgctgaa agcgaaaagg ccaatacaat agaaaagagc atatttgcca
     9901 tatcgcacct ctctattatt agattaatgc cagcgataag ccaaagtcaa taaaatctgt
     9961 tttcgagagg aaaactgatc tgcacaaata agaaatctag gcaaagatca tgttgttcat
    10021 tgcacaccta tttgatgata tggcagcgga accatgttct tagcattcgt gagtacggtt
    10081 acataggtcc gtatgatgca gtaggaaatg tggtggatct cgatcttccg gcagatattg
    10141 ctccctatcg agctggtgga gccggttatt ttggttttca gaagaatacc tcataggcct
    10201 ctttgtaggg cagcatagac ttgaaaagaa cctttagttt ctagtgcgaa cactacccat
    10261 tatcacggat gaactgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnact
    10381 gccgaatttc cgatagagaa gctctggcac gcattggagc taatgcacat ctgacgctct
    10441 atcgaacatg ggaaaatata atggacaaag ttacacagag atgtttttct atcttcgagc
    10501 gttgggagca atgaggcctt tgggaactct gtggcgcctt ggacgagagc ctttgcttgc
    10561 tatgtcagaa atgaatagca acgagaaata atataaatta tgtaatgtga caaagcataa
    10621 atcaccgcat aaatcacctc acatttcata aggaaagaga taaggttgat tatcttcgag
    10681 acgttttgtt atggttctta ggattacctg cagatcccgc gattcagtca caaaatcgag
    10741 ggagtctccg gggatagtaa gaattggggc aagagtaaaa gaggaaatcc attcctcata
    10801 gagattgttg agtccttcga gatacgaatc gggaatcgat gattcgaaat ctcgcccgcg
    10861 ctgctttatt ctcgactgca gagtagatac cgaagcacgg atgtatatca caaggcttgg
    10921 gtgaggcaag atttcggcaa gggttgcata tagggcttta tagatacacc attcgcgctc
    10981 ggccatgatt ccctggatat aaagattcct ggcaaagatt tcggcatcct cgtagatcga
    11041 tctgtcttga acaacggaat gcggatcttc gctcaatttg cggtgcatat cagctcgatg
    11101 agacagaaag aaaagctggc tatggaatgc ccatcgtgac atatttgcat agaaatcctt
    11161 gagaaaagga ttttccagga ctggctcaag ataagctata aatcctagat gctcggcgag
    11221 caggctcaca agtgtcgatt ttcctgcacc gatgttgcca gcaaccacaa catatctgcg
    11281 actcatttca atcgaagact aatctctaga aagcaaaggc gcaagcctat aaccaaggta
    11341 tggtgtaggt atggtgttca attgtaaagc atagcaaagc attgtaagca ttgcagcgca
    11401 ccgttgcttt ttaggacaaa gataagtatc ctctctcata tagtacgaga gaatatatcc
    11461 ctcaatggga aggagtctta ttcatgaaac gctttgtaat cttattattt gcatggctcg
    11521 ctgtgattgc ttctatcggt gcgcagcaag ctccagccgt ccagtcaccc tattcagatg
    11581 ctgcgcctca gacgaaagaa atcatcgatg ctgctcgtct cctggaagcc aaaggtcaat
    11641 attcaaaagc ctggaatcta cttgctgcct tcgaccctga cagcaaagat ggttatgttc
    11701 tcgccgagaa gattcgaata gccctggaag acagcattgg caatggtatg ctagtcagtt
    11761 ttagtttcgt cgatttagcg gaagggcaga gcatctatga agcatttttg aacccacccg
    11821 aaaagcagac aattgtggat ttcaatcctc tagatctagt cgaagcgcta gaaaaatcca
    11881 gcgaagctat accacccatt ctctcccttg aattagcagg ttacctttac gccgtaaatc
    11941 agcaatatag cgataattgg atgatcgatg gctatactat tcaacaaaag gcggtagaaa
    12001 actacgatag agctctcgct tatggcctat ataacgacaa gagtcttgca aatcacgccg
    12061 aacttctaat gaatcttcag cagtatgaag gtgccgaaaa ggtactaaga caagctattt
    12121 ctctatttcc cgaggagctt agctttgcgg taagccttgc aaacagtctc aactaccagg
    12181 gcaaattcga ccaagtttat tctgttatcg ataaggtaat cgaagatcct gatgtcacta
    12241 gttccacgta taatgcctat gtggaaggta tcaaggccgg cctcaattct ggtgatacgc
    12301 agaagactga ttcatatgtc gatgcgatga tcgagcgctt tccggatgaa tatgccccaa
    12361 tgctgattca gcatcttgtc gcagtaataa tgggcgaaat agaagaagcc aatgtctctg
    12421 cggacagcgt taccgaaaaa ttcaacgtcg atcccaatgt cgtacaatcg ctgctatcta
    12481 cctggcttgg tgcttcagac ccggtttcgg gcttcgatta tctaaaccgc tcgcttgaaa
    12541 agtatacgag cgatgatcaa gcaatgggaa ccttgttctt ctatagagca cttatgtatg
    12601 cacagacagc tcggtcagct gatgatctca agctagccat tgtcgacatg gaggaaacag
    12661 agaagcgctt tagtgccgta tatgataaag aagatcctgt cttccagact attgaacagc
    12721 tcctcacaga atggaaacag attatagaac agctccaaga gagtgatagt gcttcgtcct
    12781 ccgagcagtc cactccccct gaagattcga atacggcttc tgggacgagc aactgaactg
    12841 atatttgcaa aaagagacta tgcaatcggt cttttgcatt cacaatgcga ctgtcattgc
    12901 gggctatgcg cgcatggacc gcagtgctgt gcttgtcgag ggcgattcaa tcgccgatgt
    12961 tttctctgag cgcagatttg cgcaaaagtc ctttgggcca caggtgcatt tgatcgatgc
    13021 cgggggcgcc tatcttactc ccggctttat agacacccat attcatggaa ttggaggctt
    13081 tggcacagaa gacatgtccg ccgaggccat tcttgcaatg agcgagatac tacctgcttg
    13141 gggagtgagc tcttttgttc cgacattgta tcccatgccg gaagaagata tgatccgggc
    13201 aataagagcc gtggtcttgg cgatgggtca tgaaacagga gccgagatta ttggaattca
    13261 tatagaaggc ccgttcatct cacccgctca acttggggta cagagaatag agtttgtcag
    13321 gcctgtggat ctggagctaa tgcagcgcct ttgggatgct tcggaaggtc atattgtcag
    13381 catgaccgta gcgccggagc tcaagggaat gcgcgagctg gcgctgttct gcaatcagtt
    13441 gggcattgtg cttcaggcgg gccataccga tgctagctac gaaaacatga tagaaggcat
    13501 gcaagccgga ataaggcact ccacacacat gttcaatgcg atgagtcggc ttcatcatag
    13561 aaaccctaac gcagtcggag ccgtccttat ccagtcggat ttatcttgcg agattatagc
    13621 ggatgggttg catgtgcatc ctgatctgat tcgccttctt ttccgagaca aaccggaagg
    13681 caatatcgtg ctggttacag actcgctcaa accgacaaag caagcggcaa atgctcccat
    13741 gtttgccaat gatgaggaag tataccttga agacaatctc ttctatagag caagagatgg
    13801 agtgattgct ggcagttctc ttaccatgat cgaaggtgtc ctgaacctgg tcagattcgg
    13861 agtgccgctt gagagcgcag taaagatggc aagtgcaaat ccggctcgaa tattcggcct
    13921 tagaagacgt ggtatgatta gcccaggata taaggcggat cttgtgctcg caaatgcgga
    13981 cttcgatgtg cagatgacaa taattgatgg caaaataaaa tatgatcata atgctattga
    14041 aaggcgaata atatgagagt tgtcattcag cagagctatg aattgatgtc aaaatgggca
    14101 gcttattatc tggtacagca cataaatgaa catgattctc agaagccatt tgtacttgga
    14161 cttccaacag gctcaactcc gcttggaatg tatagagaac tgatcaccct ctgcaaagct
    14221 aaaaaagtca gctttaagaa tgtaattaca ttcaacatgg acgaatatgt tggccttacg
    14281 tccgaacatg agcagagcta tcatcgtttt atgtgggata attttttttc tcatatcgat
    14341 atagaaccaa aaaatgtaca tattctcaat ggtatggcca tagacctgca gaaagagtgc
    14401 actgcctacg aaaaggcgat atgcgatgct ggtggaattg atctattcgt cggcggctta
    14461 ggtgcggatg gacacatcgc gttcaacgaa cctggttcat ctctatcatc gcgtacaagg
    14521 atcaagacac tcacccggga tacaaagaaa gccaatgccc gcttctttgg tggagatcca
    14581 gaaaaagtcc cgtcacaggc tcttaccgta ggcgtaggta caataatgga tgcacaggaa
    14641 gtcatgattc ttgtatcggg aatggaaaag gcaagagcgc taaaacatgg catcgagatg
    14701 ggcgtgaacc atatgtggac aataagttgc attcagctgc acccaaaagc catcgtggtc
    14761 tgtgacgaag atgctacaaa tgagttgcgt gtagtcacag tacgttattt caaggagatc
    14821 gaagctccaa atctaagaaa ctgggatttg gattaaggtt tgaaacatca gctcagactg
    14881 gcttttgtac gaagattact atgagaagcg atataaaagg cataatggcc atggtggcga
    14941 cctctgtttg ctggagtcta agcggcttat tcattaagct catcgattgg catcctattg
    15001 caattgccgg agcgcgaagt cttgttgcag cactctttat ttttgcggta gtacgcaagc
    15061 caaaattgaa cttcggtaag aaccagatta tcgccgcgat atcttattca tgcactatgc
    15121 tcctattcgt ctacgcaaac aagaatacaa ccagcgcaaa tgcaattctc cttcaatatg
    15181 gtgcgccagt atatgtaatg ctcctttcgg gtgttatgtt aaaagaaaag cctcttccag
    15241 agcagatagg tgcgctcatt gcgattgtaa tagggatgtg cttgtttttt gccgattcgc
    15301 tcagccttgg ccatataaag ggcgatgtag cggcggtctt ggcaggtatg acctttgcaa
    15361 tccatatgct ttttatgcga caacagaagg aaggctcgcc cattgaatca ctgtttatct
    15421 cccattgcat gacagctatc gtttcacttt tgatttgtat cttcttgcct ccgcctgtca
    15481 tcagcaagag atctgtggct gccattctcg gccttggcat agtccaaata ggtttttccg
    15541 ccgtattttt cagttatgca ataaagcgca taaccgcaat ccagagttct ctcatatcta
    15601 taatcgaacc agcgctaaat ccagtatggg tatatctcgc tatcggcgaa aagccatcga
    15661 gaatggctat cttaggggga gtgattattc tttccgctgt agttatatct tcgatattta
    15721 gtgtctccag aagtgcgcga ccctcgagca tggacggagg aacctgaagc gaacctacat
    15781 gaagctgcat aacgagaaag tcatatgaag cagataattg cgctgctaac tagttgagct
    15841 gtaagctatt tataatagct aaaaagacta agaatataaa tcaaggaggc ggaagctaag
    15901 gactatgagg cagagttttc tttccgtcac aatcctcctc atactgatca ccgatccttt
    15961 agggaatatt ccactattca ttgcatcttt gaagcatgta cctaagggac ggcgttcgga
    16021 agtgatctta cgggagtgtc tcatcgcttt tctggtcttg gtgggatttc ttttctttgg
    16081 tgaggtattt ctaagcgctt tgaatctatc agacgatatc cttcgcatat caggcggggt
    16141 aatccttttc ttgattgctt tgaacatgat cttcccagga accggcggga agcttgtcga
    16201 ggatgagatc gagggcgagc catttattgt gcccgtcgcc attccattga tagcgggacc
    16261 aagcgccatt acctatgtca tgcttttgat gaagtccgat acttcgcgag cgcttgaatg
    16321 ggttggagcg atctttatag ccattcttgt caccatgata gcgtttttta tgtcgaacaa
    16381 attgaaggag tggcttggtc caagggctct ctcggcaatc gagcggctta tgggcttagt
    16441 gctgacaaca attgcgatag agatgcttct tagcgggctt ttgcaatatc tcagcagcgt
    16501 tcaaaatctt tgatgtattc taaaccggga catactgttg tagcctagac ctaagacatg
    16561 cccctgggga aatagagcta tatcaaaggc tctgaacatc aataatcaga agctaaccga
    16621 aaatctaatg tagttgccct ttggtatctc atcggtcttt aggactcgat cgacgcgagc
    16681 agctgcaggt cccacctgaa gccaggaata gaattgctcc agagcttgag gatctccctc
    16741 cgcccatact tctacatcac catcctcggt atttcgtacc cagcctttta accctaatcg
    16801 ttcagcacga atcactgcgg acatacgaaa tccaacgcct tggacttcgc cctttataag
    16861 cgcatgaaat gccttcatgc tacgattcct atcctattcc tatatagatc agggctccga
    16921 tacaccgata cttttttgct cttgagctcg tacaaggaag atattaagaa cgaccataag
    16981 aagcgaaaag agagcataga ttgcgaaccc tatgcgaatt ccccatcctg ttgagacagc
    17041 cgacatcata tatggaaata aaaatccacc tatgccacca cccattgccg caaaaccaac
    17101 agcctcactc tgagcatgaa ggaaataatc accaatgata ctcatgatag ttggatagat
    17161 aatagagcaa ccaaagccag caaggataac caaaattccg gcaataacac tggcaagctt
    17221 tccatctcca ataaagccga tcatattgaa agctatcaaa gaaatggcca tcagtgtcga
    17281 tagagctatc aagatccttt cccttttgat ggatttctga attattggaa ttccaaaacg
    17341 tcccattagc agccctgccc agaatagaga gaccatgaag gaacctgtgg gtatgggagc
    17401 tccgaaaaca cttacaaaat actcagcaat ccagttcgaa atacctagtt caacccccac
    17461 atagacaaac aaggcaaaaa atccaagcca atacatcggc tgatgaaaaa gatcgcgcct
    17521 cgatctattt gttatctgtc ctctgccctt tccgagcaac ttaaaaggca tgaattgcat
    17581 gaaaatcgca ataccaagga ataatacacc cataattcga taaacgagcg tccattccag
    17641 attggcactg atcaatatcg ctattactag tggtccgact acggctccaa tcgaaaaagc
    17701 accatgcatc aggctcatag cttttccacc cccaggacct tccatgcgga gtacagccca
    17761 gtcgactgca atttctattc caccctggcc tactccaatt ataaaataga gcatcgtatt
    17821 gagccatgct gtaggtgtct tggcgaagaa ttcaagcccg cagcatataa ggatcagcga
    17881 taaggaaatc aaaaagcgga gccccaggaa ggggaagaga attccagcaa ggtaactcga
    17941 tagaaaatat ccaaccgaac ccgccgctat gaccattcct gccgtcatat aagaccagcc
    18001 aaattccgcg aatatctttg gaagcgtagc ccctatgact gtcatggaaa taccaaaaag
    18061 ggcaaagaca gcaaagagac atatgtaaag ggataaaagg cgtttttcta ctatcatgat
    18121 gctatattgt gcgatattcc ttcagttttc gtcaatgaga gccttcaggg cagaggtcaa
    18181 agatggcgag gcaattaccc ttgcgcaaat ttgtcgcaca gacaaaa
//
LOCUS       DAOOWY010000032        18052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3124_length_18052_cov_10.867145, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000032 DAOOWY010000000
VERSION     DAOOWY010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 18052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18052
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3124_length_18052_cov_10.867145"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PK105_04010"
     CDS             <1..667
                     /locus_tag="PK105_04010"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOU60904.1"
                     /translation="DSHARGEGIPFRSFGDSRVTLIRGYPRPFYMLEGLDESFDLLML
                     VGYHAKIGALHGLMDHSYSSSCIYCVRLNGQEMSEADINGMLASHYGVPICFVSGDDI
                     LEKEIEEHFSILPIFVCTKQGLGRFAAKMYFEENLKPEFVEGAMQAIDRKDVFKPLNL
                     APVYDLEIDFASTAIADAVSVIPGLERMEGRRVLYRSTEMKSIYRMIHASAMLGGKFA
                     AFT"
     gene            781..2529
                     /locus_tag="PK105_04015"
     CDS             781..2529
                     /locus_tag="PK105_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU60905.1"
                     /translation="MKKFLSIVLICLALGGMVFAQAKKGPTLRNRDPDTLVFVLAAAP
                     QPLDPNIYTTLNEAQIMREINETLVGMKSDNTFYPVLAETIPTIQNGLVSKDGSVYTI
                     KLRKGVKFHNGNPFTADDVVFSFNRILHHPKSSSKSMFSAVLSVVKVDDYTVKITFGR
                     LKDPEYATIALTSWEDRAKYMTASPYGPALNILAHYCSGIEDKETVEAAGADYGTKIV
                     VGTGPYKFVSWPNPQEVQLTRNEEWWGGAKSVAYKNVVFRAMSEQPQVNNVLITGEVD
                     MAFNLSKLDIGNLDRNGLVITTKPGVTIWYGVFNMNSDLVGQKKNGKLDLSGQYTEGD
                     STHLRRAIFYSINPVPFIKQVDIFNGYAVPANQMMQPGFLGYVEGAPAGTPFAEFNEK
                     TWYFNREKARSEFAMLSPEFRAKLKPESVTLVVGNVTDRVKIANNIKDQVKTTLGVDL
                     IKVVPMVNAQIIQQSKTGDGYDMVVTAWFTPTMDPDYTCIIYGGDSIGTGMNGALYNN
                     DIVNKNIQIGRYSLDRKIREEAYIKVQTQLMTDKPYFPMVYENVLFASHPRVGNLDQA
                     IFKTKLVDIHLLTKLQ"
     gene            2634..3623
                     /locus_tag="PK105_04020"
     CDS             2634..3623
                     /locus_tag="PK105_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60906.1"
                     /translation="MFRYIMRRILMLIPVLLGVTFIVFSLMYITPSDPAQMMLGANAS
                     PAAYKQMREDMGLNAPFIVQYGRFLFGYKHPIFGYKGLLFGDLGRSYVSNRIIFELIF
                     SSFPNTLILAVGALILAIILGIPIGIISATKPYSVADMVFTVFALLGASMPVFWIAMV
                     LIYTFANNLRLFPAIANPRDLLSLVLPTITLSMNSMAIIMRMTRSSMLDVMQQDYIRT
                     ARVKGMDEKTVVFKHALRNALIPVVTVAGLQFGSLLGGAVLTEKIFSYPGLGSIMVDA
                     IFQKDTPQILGSVCFVAITFTIINLAVDILYGFIDPRIKSAFSKKKKTVRGDL"
     gene            3620..4540
                     /locus_tag="PK105_04025"
     CDS             3620..4540
                     /locus_tag="PK105_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60907.1"
                     /translation="MSNQSFLRFLKRQSKNISQFWRQFSRNKLAVASLILVLIFALVA
                     IFADILYSYDFVTSNSLENALLKPGETSTVYFQEKDIEHTFILGSDNYGRDILGRLVH
                     GARISMIIGFVTVAMAVVIGGILGAIAGYKGGLFDTLIMRLTDIILSIPTILMSIAIV
                     AALGNSFFNLLIAISTSLIPGYIRIVRASIISIKDQEYVEAARVIGAGDFRIVLRHIL
                     PNITSPIIVQSTLNVGSSILSASWLSFLGLGILPPKPEWGNMLSEAREFIQYAPHLLF
                     APGIAILLTVLAYNIIGDGLRDALDPKLRR"
     gene            4551..5525
                     /locus_tag="PK105_04030"
     CDS             4551..5525
                     /locus_tag="PK105_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60908.1"
                     /translation="MDKILEIKDLHVHYFSNRRTVKAVNDLSLDMQRGRVLGLVGETG
                     AGKTTLALSILNMIRAPQGKIISGSIRFNNTDILGMSNQELREMRGKDIAMIFQDPMT
                     ALNPVLTIGEQIAETIYKHEKISKAEAWSKSEEMLETVGIPGVRAKEYPHQFSGGMKQ
                     RVVIALSLACNPTLLLADEPTTALDVTIQAQVLDLMRGLIKTNNTSVLFITHSLGLVV
                     ENCDEVATMYAGEIVEYGTVEQVFGNPLHPYTRGLLGSLPDYSKGSPRLRPIPGLMPD
                     PTNLPKGCYFSPRCPNVMPECHEVHPQNTSIEGHLVKCHQCQENLHVE"
     gene            5515..6489
                     /locus_tag="PK105_04035"
     CDS             5515..6489
                     /locus_tag="PK105_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU60909.1"
                     /translation="MLNNQSELPILRVKNLKKYFHVARGTLHAVDDISFDIKRGGTLG
                     MVGESGCGKSTTGRLLVRLHESDGGSINYGGVEITKAPQKEMKTLRTKMQMIFQDPYS
                     SINPHHTVASIIGEPLKIYKKVRNMQEYEEKVRSLMETVGLADRLYDAYPHELDGGRR
                     QRIGIARALSLEPQFIVCDEPVSALDVSIQAQILNLLMDLQDSLNLTYLFITHDLSVV
                     KHISDEIVVMYLGQIVEKANTDELFKHPIHPYSKALESAIPIPRLNARKERIILKSEV
                     VSPIDPKPGCRFAPRCNYAVAACSESDPILREICPGRFVACHRAEMFV"
     gene            6526..7521
                     /locus_tag="PK105_04040"
     CDS             6526..7521
                     /locus_tag="PK105_04040"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOU60910.1"
                     /translation="MVPIFDMHADIIMDILRREDISDENSVLLRFKQHIDRMKRGAVQ
                     GAVLVDCRMAGESAETKNFEEFIAISKILRSHQDESYLIATSSEEFDNSIRDGRWVGI
                     LCYEGLRAANGNLLWLKRLYKEAGLRIAMLTHNDSNEFATGAADPNGNPASKNPLGLT
                     EAGQKAVELMNEMGILIDLSHASPMTRRDVLSYSKKPVMLSHTSSAEVYDNGRNLSDK
                     DIREIAEHGGLIGCMTSPAALADIRDRANHTLERYMHHLRHMIDVAGEDHVGLGLHFC
                     EYLYTQEEYPPVEGLEDASKAGVILKEISKWGLSTYQIEKIAYRNFARVFGGATD"
     gene            complement(7614..8351)
                     /locus_tag="PK105_04045"
     CDS             complement(7614..8351)
                     /locus_tag="PK105_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOU60911.1"
                     /translation="MFAQNLTFPHFEEQIKKIDTIVIPTGSLEAHGRHCPLGTDILIP
                     ERLCKDLELAMGDRILIAPSINYGYSPLLRAFPGTIHLSAELLISIYSEVAKGFIRWG
                     AKNIVFMNGHGGNIPMLSVACDRIAEAGGSAMVISWWATFSQEILGICSSQGHAGEDE
                     TSVMMAIDATLVDQKSRSSHVKKAFSLPLATPDQVAERYPDAMNGNSLLATKEKGERL
                     LAMMLEKNIEYIDRLRRKDLLVPIEIS"
     gene            complement(8382..9032)
                     /locus_tag="PK105_04050"
     CDS             complement(8382..9032)
                     /locus_tag="PK105_04050"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOU60912.1"
                     /translation="MKADNERQQERDFMVRFQIAARGIRDKRVLDAMRRVPRHLFVPS
                     SYRVEAYEDYPLPIGSGQTISQPFIVAFMAEALLLKGDERILEIGTGSGYQTAILSLL
                     AKEVYSIERIDALLDRARTILESLEITNVELRLADGFYGWEEKAPFDAIILSAAPFDI
                     PSLLLQQLADGGRLVGPVGPLGAQTLVRVTKHGQHFETETLLDVAFVPMRSGLSKV"
     gene            9217..10263
                     /locus_tag="PK105_04055"
     CDS             9217..10263
                     /locus_tag="PK105_04055"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60913.1"
                     /translation="MRLLVIMKRNLDQSLAEQRNARYLHMIEIYKHAGFEPYLSYAES
                     IDDIKLSLNNHLPDLVLCGIDHLPENGCNISHNVHAWFEEHNVNYIGSDYKVIELALS
                     KGLLKQKWQTYGIRTPVFGLIREEPEECNEDIESLLSLNAFPYILKPENLGNSRGIDE
                     NSIVWNEAELRKALDKMRLQFGGVILAEHYLGASADFREITCAMIEGSSGMLFMPAEV
                     SLVEPKRFHIITTRDKDKKGTIANPLDADTAASFLPFAERVFAIAGVRDYSRGDFILA
                     DGEFWAIEINGQPMIPDQWFGSAALFAGLSEEKYLVGIVASGYRRLKAEGRLAQVFPK
                     EAQALLKGTKLEVL"
     assembly_gap    10285..10384
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10385..12346
                     /locus_tag="PK105_04060"
     CDS             <10385..12346
                     /locus_tag="PK105_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60914.1"
                     /translation="EMYRVRQTDVVGIVKPAIDAHTLGILSFAQILRDCGIRVEIAGD
                     DVNKDIDRITNEGQLDSLVRWIRDRHIDILGFSYRLDPERGAEIFARLVERLRRLGLM
                     AREGGPLVALWFAGLPPACRRAAERVPMVDATFQGDESYEEVLEKLGLPAHLVPEQAS
                     VELAYDKARLELGKEIVKNRKYLEIKPIDRSGSKRFGLRGERVLDRVHHGIEQHLPPL
                     MRAHVGPYAEDREQAVALFLDWTKQLAKTRMLDILSIGSSQLTQSHFGEDWTGLSNGG
                     GVPINNPAEYREVWEAARPMLVRTYAGTKHVPEMAKMHEEALDICWHALSFWWFSLLD
                     GRGENTVLQNLEEHFEALRYIASTDKAMEPNVPHQFAFRGCDDVGYIVSGYVAAKAAK
                     AQGIRTLILQIMLNTPKYTWGIQDIAKARALRTLVGELEGPDFAVILQPRGGLDYFSS
                     DLEKARAQLAAVTMLMDDIEPDNPASPQIIHVVSYSEGVRLADPEVVEESVQITRYAL
                     KEYRRLKKKGDMPEYGKSAMVEARTRSLLKEAKNMIKFIEATIPNTYSPRGFYSMLRS
                     GVFPLPWLIACKDEFEAAVNQPVQFLHGGVHVVDREGKPLSVSQRLFGIARNLEDIGF
                     APSITHSADSSWLASGSGGGQKEQYGEKH"
     gene            12333..13475
                     /locus_tag="PK105_04065"
     CDS             12333..13475
                     /locus_tag="PK105_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOU60915.1"
                     /translation="MKNTDWEGIVTHLDHIAAKGKKMVWCVVGAGNGGIAMAGHLGIM
                     GFPVQMYNRTDEHLNAIRWYGGIDIEGMVTGFGPVRLASANMGDALEGSDVIMIVTPS
                     TAHYNLATLMAPFLKDGQIVVLNPGRTGGVLEFRAELERQKCEASVVLVEAQTFVYAS
                     RMITRNRAHIFSIKNGVPVSALPSFLTPAVLKVIDVAFPQFIAGSNVLATSIENIGAV
                     FHPALTLLNAGWIESTGGDFEYYLQGISPSVAMVLQAIDDERLALARALGIRTVSARE
                     WLYLTYDSVGDDLYTAIRSTLAYAGIKAPRSIEHRYIWEDVPMSLVPMASMAAMYGIE
                     TPAINLIIDLANLMLSQDYRANGRTVHSLGIEGMSVEELHRLVTEG"
     gene            13503..14186
                     /locus_tag="PK105_04070"
     CDS             13503..14186
                     /locus_tag="PK105_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60916.1"
                     /translation="MNETLIAITQWIDNLPSGIGAILILLVGWAIAKLLKLLVPIILA
                     WLKFDVLAEKIGIKEFLRKGNVRYSASKLIGVFLYWVVMLFALAEAASVLDKRAAETI
                     SSWLYGAIPSILATIITATIGIFIVNFLSNFVMTIMSNAGLSSAALVKSLIRVVGYII
                     VIIMVVEQLGFESIMNVLLLLLIGSAALGVAIAIGLGCKDIARQYVDSFLRSLRERER
                     ASHGSDLEG"
     gene            complement(14206..15135)
                     /locus_tag="PK105_04075"
     CDS             complement(14206..15135)
                     /locus_tag="PK105_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU60917.1"
                     /translation="MADQQNIDIKDKRYSIYNSEIPMPNPSTTTTTDNFDIEKEIDGN
                     RYAEIVTRSKKPFFAILRAAIKEDFRFMLPRILMRTAKTMRRIPRTFVIKTQEELDQY
                     VFETIFLWSRSLIKITKMEIEVRGADKILPDRTYLFASNHRSPADIPVLFASIPQPAA
                     FVANDLFGRIPALSYWMRASGSVFVNQGLPQSEVRALKAMEKRLKKGRSLILFPEGHM
                     HQGNGIDEFNRGGLFAAVRCAVPIVPICIYGTEKVMRPGSFHINPYRHVVVEFGEPIE
                     TKNLCRADKKGIHLAVHDIIKNMRTALEQEFSS"
     gene            complement(15245..16501)
                     /locus_tag="PK105_04080"
     CDS             complement(15245..16501)
                     /locus_tag="PK105_04080"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOU60918.1"
                     /translation="MPRTEQEYKAAREIYAELAIDTDQAIETLASIPLSIHCWQGDDV
                     IGFDGATSLSGGILSTGSYPGRARNAEELRSDAAFAFSLIPGKKRFNLHAMYAETGGA
                     KVDRCDLEPSYYEGWIEWAKQQHIGLDFNPTFFSHPLAESGWTLAHADNNIRDFWIRH
                     GKASRRIAQSIADALKDHVVNNLWVPDGYKDMPADRLGPRLRLKESLDAIYSEKLPND
                     RVLDSVESKLFGIGSEAYVAGSHEFYLGYASQKAIGLCYDMGHFHPTENVADKISSTL
                     LYVPYLILHLSRGLRWDSDHIVIWNDSLTDVCREIVRMHVWDRVHLALDYFDSSVNRI
                     SAWINGARSTQRALLYAFLEPLEPMRAAEEQADFGTRLSYIEESGSLPFGAIWHHYCE
                     TQNVPYGLGWLEDVKDYEKRVLAKRM"
     gene            complement(16530..17717)
                     /locus_tag="PK105_04085"
     CDS             complement(16530..17717)
                     /locus_tag="PK105_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOU60919.1"
                     /translation="MNLDSIIRLSRRYGADSRYVLAGGGNSSIKQDNVMAVKASGVSL
                     GTIDERGFVLLSLPKLINILEAPLSSDATDPEEIVLERLLASRLPGQTMRPSVETLLH
                     ALFPFRLVVHLHPALVNGLMCSVEALKRVPELFGEEAEVLPYTTPGYTLADRVRTLFR
                     TRKAMGRCPIKILFLRNHGVFVAADNADEIDDLYRYIFDTLGKWVRPLPLDDDSHSVA
                     SKVKNELPSEDIRVMIEAISSEFNVRFSFTLNSLLGYFLGSPEAFEPLSQPFTPDHIV
                     YAGAWPLFLSQSAASDLKQIKSAIHEYIANHGELPKILAIQRTGIFGLGKDDNAAERA
                     CLLFQDAAKIAWYAQSFGGVHPMESEDADFIRGWEVEKFRSGVAANVLTSPSSESAPI
                     LKK"
     gene            complement(17736..18050)
                     /gene="rhaM"
                     /locus_tag="PK105_04090"
     CDS             complement(17736..18050)
                     /gene="rhaM"
                     /locus_tag="PK105_04090"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOU60920.1"
                     /translation="MKRYAFVMQLKPGNEAEYKRRHDEIWPELKCELRKAGISNYSIY
                     LDPETLRLFAIQSLAEDETSDSLSELPIMRKWWDSMKDLMDTNADNSPKIWSLKEMFH
                     MD"
BASE COUNT         4787 a         3914 c         4354 g         4897 t
ORIGIN      
        1 cgactctcat gctaggggcg aggggattcc gtttagaagc tttggcgatt cgcgagttac
       61 cttgattcgt ggctatccac gtcctttcta tatgctcgaa ggcctggacg agagtttcga
      121 tcttttgatg ctcgtgggat atcacgcaaa aattggggcc ttgcatggat tgatggacca
      181 tagttattca tcctcatgta tttattgtgt acggttgaat ggccaggaaa tgtccgaagc
      241 ggacataaat ggcatgcttg cttctcatta cggagtaccc atatgttttg tgtccggcga
      301 tgatattctt gagaaagaaa tagaagagca tttttcgatt ttacctatat ttgtttgcac
      361 aaagcagggt cttggtcgat ttgcagctaa gatgtacttc gaggaaaact taaagcctga
      421 gtttgtcgaa ggcgcaatgc aagccatcga caggaaagat gtattcaagc cacttaatct
      481 agcgccagtt tacgatctcg agatcgattt tgcctctacc gcgatagcgg atgctgtttc
      541 tgtgatacca gggttggaac gaatggaagg caggcgcgtg ctctataggt ctaccgaaat
      601 gaaaagcata tatcgaatga ttcatgcatc cgcaatgcta ggcggtaagt ttgcggcttt
      661 cacttgataa aaaacaccta tacaagcttt ttattccttt tgggagaaaa acttatttat
      721 atggaaaaga aactaaagct gcgttgcagt tatagaatat cttcaagggg ggttattgac
      781 atgaagaaat ttctgtcgat tgtacttatc tgccttgctt tgggtggaat ggtttttgca
      841 caagcaaaga agggaccaac tctgcggaac agagatcctg atacattggt ctttgtcctt
      901 gctgctgctc cacagccgct cgatccaaac atatacacga ctctgaacga agcgcagata
      961 atgcgagaga tcaatgaaac actggtgggg atgaagtcag acaatacctt ttatccggtg
     1021 ttagccgaga cgattccaac gattcagaat gggcttgttt ccaaagacgg ttctgtctac
     1081 acgattaagc tccgaaaagg cgtaaaattc cacaatggca atccatttac cgccgacgat
     1141 gtagttttct cattcaatcg aattctgcac cacccgaagt cgagttccaa gagtatgttc
     1201 agtgcggttc tttccgtagt caaagtagat gattacacag taaaaattac ctttggcaga
     1261 cttaaagatc ctgaatatgc gactattgca ttgacctcat gggaagatcg cgcaaagtat
     1321 atgacggctt ctccatacgg accagctctc aacattctgg cacactattg ctcgggtatc
     1381 gaagataaag agacagtgga agctgcgggt gcagattatg gtacgaagat tgttgttgga
     1441 actggcccct acaaatttgt gtcatggcct aatccgcagg aagtccagct tacgcggaac
     1501 gaagaatggt ggggaggcgc caaatcagtt gcttacaaga atgtggtctt tagagccatg
     1561 tcagaacaac cgcaggtcaa caatgtcttg ataaccggcg aggtagatat ggctttcaac
     1621 ctctccaagc ttgatatagg aaatttggac agaaatggcc ttgttatcac aacaaaaccc
     1681 ggtgttacga tatggtacgg cgttttcaat atgaattccg atttggttgg gcagaaaaaa
     1741 aatggcaaac ttgatttatc tggccagtac actgaaggcg actccactca tcttagaagg
     1801 gcaattttct attcgataaa tccagtgccc ttcatcaagc aggttgatat cttcaatggc
     1861 tatgcagtgc cagccaatca aatgatgcag ccaggattct tgggctatgt cgaaggtgct
     1921 ccagcgggta ctccctttgc agaattcaat gaaaagactt ggtatttcaa cagggaaaag
     1981 gcaagatccg aatttgccat gctttcgccc gaattcagag caaagctaaa gccggaatca
     2041 gtcaccttgg ttgtgggcaa tgttaccgat cgagtgaaaa tcgctaataa tatcaaagat
     2101 caggtcaaga cgactctcgg agttgacttg ataaaggtag ttccaatggt gaacgcgcag
     2161 attatacagc agagcaagac tggcgatggc tacgacatgg tggtaaccgc ctggttcacg
     2221 cccactatgg atcccgatta tacatgtatc atttacggcg gcgactctat aggaaccgga
     2281 atgaatggcg cactctacaa caatgacatt gtcaacaaga acattcaaat tggccgctat
     2341 tcactcgatc gtaagatccg agaagaagcc tacataaaag tgcagaccca gctcatgacg
     2401 gataagccgt atttcccaat ggtatacgaa aacgtgttat tcgcgtcgca tccaagagtg
     2461 ggtaacctgg atcaggcgat attcaagaca aaattggtcg atattcatct tttgactaag
     2521 ctacaatagt ccttttgaga tgcatgcctg ctccatgcaa gcggggcagg tatgctttta
     2581 aaattctgat atcgccattc gttccagatc aatatttccg aaaaggttct gttatgttca
     2641 gatatataat gcgtcgaata ctgatgctta ttccggttct attaggtgtg acattcattg
     2701 ttttttctct aatgtatatt actccctcgg atcccgctca aatgatgttg ggagcaaatg
     2761 cttcacccgc agcatataag cagatgagag aggacatggg tttgaatgcg cctttcattg
     2821 tgcagtatgg aagatttctc tttggatata agcaccctat ctttggttat aagggactat
     2881 tgtttggaga tcttggcagg tcctatgtgt cgaataggat aatattcgag ctcattttca
     2941 gctcttttcc taatacgctt atccttgcag tcggagcgct aattcttgct ataatactgg
     3001 gtattccgat tggaatcatc tctgcaacaa aaccatactc tgtggctgat atggttttta
     3061 cggtttttgc tctcctggga gcatcgatgc cagtattctg gattgcaatg gtgctcatct
     3121 atacctttgc caacaactta cgcctttttc ctgccatagc caatccgcgg gatttattgt
     3181 ctcttgtgct gccaacaata actttgagca tgaattcgat ggccatcatt atgagaatga
     3241 cacgttcatc aatgctcgat gtcatgcagc aggattacat caggacagcg agagtaaaag
     3301 gcatggacga aaagacagtg gtctttaagc atgccttgcg taatgccctc attccagtag
     3361 tcacagtcgc aggtcttcag tttggaagtc ttcttggtgg agccgttctg actgaaaaga
     3421 ttttttccta ccccggactt ggttctataa tggtggatgc aatatttcaa aaagacacgc
     3481 cgcagattct cggatcggta tgtttcgttg caataacatt caccatcata aatcttgcgg
     3541 ttgacattct atatggattc atcgacccac gcattaaatc cgctttctcg aaaaagaaaa
     3601 aaacagtgcg aggcgaccta tgagtaacca gtcatttttg agattcttga agcggcaatc
     3661 aaagaatatt tctcaattct ggaggcaatt ctcaaggaac aaactcgcag ttgcaagctt
     3721 gattctagtt ttgatattcg cgcttgttgc tatttttgct gacattctct attcgtatga
     3781 ttttgttact tcgaattcgc tcgagaatgc gcttctcaag cccggagaaa ccagtacggt
     3841 ctattttcaa gagaaagata tagagcacac tttcatcctt ggctcggaca attatggtcg
     3901 tgatattctc ggcaggctgg tccatggtgc aagaatctct atgatcatcg gttttgttac
     3961 tgtagcgatg gcggttgtaa taggtggcat tttaggagct attgctggat ataagggagg
     4021 gttattcgat acactcatta tgcgacttac cgacataatc ctttctattc cgaccattct
     4081 aatgtcgatt gccattgtag ctgcgcttgg gaatagcttt ttcaatttgc tgattgctat
     4141 aagcacatcc ttgattcctg gctatatacg aatagtccgc gcttctatta tctcgatcaa
     4201 agaccaggaa tatgttgaag cagcgcgcgt aataggtgca ggagattttc ggatcgtgct
     4261 caggcacata ctgccaaata tcacctctcc gatcattgtt cagtctacgc tcaatgtagg
     4321 ctcatcgata ctctctgcct cttggttgtc ttttttagga ctaggaattc ttccgccaaa
     4381 accagaatgg ggcaatatgc tttccgaggc tcgcgaattc attcaatacg cgccgcatct
     4441 cctatttgca cccggaatag ccatcctgct caccgtattg gcctacaata tcattggcga
     4501 tggattacgc gatgctctag acccaaaact caggcgttag gaagctgatt atggataaga
     4561 ttctagaaat aaaagatcta catgtacact atttttcaaa tcgaagaacg gtcaaggcgg
     4621 ttaatgatct ttcgctggat atgcagagag gtcgagtatt gggtcttgtt ggagagacgg
     4681 gagcgggtaa gacaacgcta gcactttcga tactcaacat gatacgtgca ccccaaggca
     4741 agatcatctc cggatccatt cgcttcaaca atacagatat tctcggcatg tccaatcaag
     4801 agctgcgaga aatgcgggga aaagatatag caatgatttt tcaggatcct atgaccgctc
     4861 tcaatcctgt gctaaccatt ggagaacaga tcgctgagac aatctataag catgaaaaga
     4921 tatctaaggc tgaggcttgg tcgaagtctg aggagatgct cgaaactgtc ggcatacctg
     4981 gcgtcagagc aaaggaatat ccccaccagt tttcaggtgg aatgaaacag cgcgtcgtaa
     5041 tagccctttc attagcatgc aatccaacgc ttcttctggc ggacgagcct acaacagccc
     5101 tggatgtcac gattcaggcg caagttctcg atttgatgcg cggcctcata aaaaccaata
     5161 atacatcggt tctatttatt acccattcgt tagggctggt tgtcgagaac tgtgatgagg
     5221 tagcgaccat gtatgcagga gaaatcgtgg agtatggcac ggtagaacag gtgtttggga
     5281 atccgcttca tccttataca agaggtcttt tgggttctct ccctgattat agcaaaggct
     5341 ctcctcgtct ccggccaata cccgggctta tgcccgatcc aaccaatttg ccaaaaggat
     5401 gttatttcag cccgcgatgc cctaatgtaa tgcctgagtg tcacgaggtt catccgcaaa
     5461 acacttctat agaaggccat ttggtgaaat gccatcagtg tcaggagaat ttgcatgttg
     5521 aataatcaat ctgaattgcc cattttgcga gtaaaaaacc tcaaaaaata cttccatgtt
     5581 gctagaggga ctctccatgc agtggatgac atttccttcg atataaaaag gggaggaaca
     5641 cttggtatgg tcggcgaatc cggttgtggc aaatctacta ctggaagatt gcttgttcgc
     5701 cttcatgaat cagacggagg ttctatcaat tatggcggag ttgagattac aaaagctccg
     5761 caaaaggaaa tgaagacact gcgcaccaaa atgcagatga ttttccagga cccatattcc
     5821 tctatcaatc ctcaccatac tgttgcatcg atcatcggtg agccgctcaa gatatataaa
     5881 aaggtaagaa atatgcagga atatgaggaa aaagtgcgat cgctcatgga aactgtcggt
     5941 ctagctgata gattgtatga tgcctatcca catgaattag atggaggcag aagacagcgt
     6001 atcggcattg cgcgtgccct ttcccttgaa cctcaattca ttgtctgcga tgagccagtc
     6061 tctgccctgg atgtatcgat tcaagcacag atactcaact tgctcatgga tcttcaggac
     6121 tctttaaacc taacatatct ttttattact catgatctct cggtggtaaa gcacatatct
     6181 gatgaaattg ttgtgatgta ccttgggcag atcgtagaga aagcaaacac cgatgagctt
     6241 tttaagcatc caattcatcc ctatagcaag gctctggaat cggctattcc tattccaaga
     6301 ctcaatgcca gaaaggaacg gataattcta aagagcgagg ttgtgtcgcc aatagatcca
     6361 aagccaggtt gtcgctttgc accgcgctgt aattatgccg tcgcagcttg cagcgaatct
     6421 gatcctattc tcagagaaat atgtccaggt cgttttgtgg cctgccatcg cgcagaaatg
     6481 tttgtctgaa agtcagttct agcgctaaca gagctggtga aatcgatggt acccattttt
     6541 gacatgcatg ctgacatcat catggatata ttgcgaaggg aagacatctc tgatgagaat
     6601 tcagtgcttt tgagattcaa gcagcatatt gatagaatga aaaggggagc tgtccagggt
     6661 gctgtcctag tcgattgtcg catggctgga gaatccgccg aaacaaagaa cttcgaagaa
     6721 ttcatagcga taagcaaaat attgcggtca catcaagatg aatcatatct gattgcaacg
     6781 tcttctgaag aattcgataa ttcaattaga gatgggcgat gggttggaat cctatgctat
     6841 gagggcttgc gcgcggcgaa tggcaatctt ttatggctaa aaagattata taaagaagca
     6901 ggccttcgaa ttgccatgct aactcataac gatagcaatg aatttgcgac tggagccgcc
     6961 gatccaaacg gaaaccccgc atcgaagaac cctcttggct tgacagaagc gggacaaaaa
     7021 gcagtggagc tcatgaacga gatgggaatt ctcatagatc tgtcgcatgc aagtcctatg
     7081 acaaggcgag atgtcctatc gtattcgaaa aagcctgtca tgctgtctca cacatcgagc
     7141 gcagaagtat atgacaatgg cagaaacctt tcggataaag acataagaga aatagctgag
     7201 catggagggt tgataggatg tatgacaagc ccagcagcgt tggctgacat aagagacaga
     7261 gccaatcata ctcttgagag gtacatgcat catttgcggc atatgatcga tgtggctggc
     7321 gaagatcatg tgggactggg cttgcatttc tgtgaatact tatatacaca agaagaatat
     7381 cctccagtcg agggcttgga agacgcatcg aaggctggtg taattctcaa ggaaataagt
     7441 aaatggggcc tttctaccta tcaaattgag aaaattgcat atcgcaattt tgctagagtc
     7501 tttgggggag cgactgattg agtttgccct atctcagcat atgtttagga aatagaatat
     7561 taaaagcaga tgaaaatctg tacccgaatt ggagttggcc aaaaatataa atcctagctg
     7621 atctcaatag gcactagcag atcttttctg cgcaagcgat cgatgtattc gatgttcttt
     7681 tcaagcatca tagccagaag tcgctctccc ttttccttag ttgcgagaag tgagtttcca
     7741 ttcatggcat cggggtaacg ctctgctacc tgatcaggcg tagcgagtgg aagagaaaag
     7801 gccttcttca catgggagct tctggatttt tgatcgacca aagtggcgtc tattgccatc
     7861 attacagaag tctcatcttc tccagcgtgg ccttgggagc tgcaaatgcc aagtatctcc
     7921 tgagaaaatg ttgcccacca gctgattacc attgcggagc cgcctgcttc tgctatgcgg
     7981 tcgcaggcaa cagacaacat cggaatattg ccaccatgcc cattcataaa tacaatattc
     8041 ttagctcccc accttataaa gccttttgca acctcggaat agatcgatat tagaagttcc
     8101 gcagaaagat gaatggttcc ggggaaagcc ctcaaaagag gcgagtagcc ataatttatg
     8161 gaaggagcaa tgagaattcg atctcccata gcaagttcca gatccttgca aagacgctcg
     8221 ggaatgagaa tgtcggttcc cagagggcag tgtctgccat gagcttcaag gctaccagtg
     8281 gggatgacaa tggtgtcgat cttctttatc tgctcttcga aatgaggaaa tgtcaaattc
     8341 tgtgcaaaca taattctctc caatgattta gtattcctgc attagacttt gctcagcccg
     8401 gagcgcatag gcacaaaggc aacatccagg agagtctcgg tttcgaagtg ttggccgtgc
     8461 ttcgtaaccc ttacgagagt ctgtgctcca agaggcccga caggtccaac gagcctgccg
     8521 ccatccgcca gctgctgcaa cagaaggctg ggaatatcaa agggcgctgc gctcaatatt
     8581 atagcatcaa agggagcctt ctcttcccat ccataaaaac catctgccag acgaagctcg
     8641 acgtttgtta tttcaaggga ttctaggata gttcgggcgc gatctaaaag tgcgtcgatg
     8701 cgctctatac tataaacttc cttagcaagc aaggagagta tagcagtctg atagcccgag
     8761 cccgtaccta tttccagaat tcgctcgtct cctttgagca agagcgcctc tgccataaaa
     8821 gcaacaataa atggctgaga aatggtctga cccgagccta ttggcaaagg gtaatcctcg
     8881 taagcctcga ccctgtaaga cgatggcaca aagagatgac gaggaacccg gcgcatagca
     8941 tcgagcacgc gcttgtctct tataccgcgt gccgcaatct ggaagcgaac cataaaatcg
     9001 cgctcttgct gtcgctcgtt atcagctttc atcctttgcc tattatatca tgatagcgaa
     9061 ctcaggattt ggctttttat gtctcgaaat gtccatatat ttcaaaagtc cagaaaattt
     9121 tagatattga caaaaaaaat tagcatgttt ctccaaattg tgttacaaat ttatatatag
     9181 gaattgagca aaaggaaaat caacgcggag gaccagatgc gattacttgt aatcatgaag
     9241 agaaaccttg atcagagcct tgctgagcaa cgcaatgcaa gatacctaca catgatcgaa
     9301 atttataagc acgctggttt tgagccttat ttatcctatg cagagagcat tgatgacatc
     9361 aaattgagcc tgaataatca tttgccagat cttgtgcttt gcggaataga tcatcttcct
     9421 gaaaatggat gcaatatatc gcataacgta catgcctggt tcgaagagca taacgtcaat
     9481 tatattggct cggattataa ggttatagag ctggccttat ccaagggctt gctcaaacaa
     9541 aaatggcaaa catatggaat aaggacacct gtcttcggtc ttattagaga agaaccagag
     9601 gaatgcaatg aggatataga aagcctttta tcgctaaacg ctttccccta catacttaag
     9661 cctgaaaatc ttggcaattc gagaggcata gatgagaaca gcatagtctg gaatgaagcc
     9721 gaacttcgca aggctcttga taaaatgcgt cttcaatttg gaggagtaat tcttgcggag
     9781 cattaccttg gtgcttcagc cgattttagg gagatcactt gtgccatgat agagggatct
     9841 tcaggcatgt tgtttatgcc tgctgaggta tctcttgtag agccaaagcg ttttcatata
     9901 ataacgacgc gcgacaagga taagaaagga actatcgcaa atccactaga tgctgatacc
     9961 gccgcctctt ttctgccttt tgcggagaga gtttttgcta ttgctggcgt acgcgactac
    10021 tcgcgtgggg attttatctt ggctgatggt gaattttggg caatagaaat aaacggtcag
    10081 ccgatgatac cagatcaatg gtttggtagt gcggctctgt ttgctggatt atccgaagag
    10141 aaatatctag tgggaattgt tgcatccggc tatcggcgac tcaaggcaga aggccggctt
    10201 gcgcaagttt ttccaaagga ggcgcaagct ctgcttaaag ggacaaaact cgaggtcttg
    10261 tagagccctc ttttgacaga ggagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnngaaatg tacagagtaa gacaaactga tgtagttggt attgtaaaac ctgcaataga
    10441 tgcacataca cttggaattt tgtcgtttgc ccagatttta agagattgtg gcataagagt
    10501 ggagattgct ggagatgatg taaataagga tatcgacaga ataactaatg aagggcaatt
    10561 ggattctctt gtcagatgga tacgcgatag acatatcgat attttgggat tttcatatcg
    10621 gctcgaccct gagcgtggcg ccgagatttt tgcgcgcctt gtcgagcgcc ttcggcgcct
    10681 cgggcttatg gcaagggagg gtggaccgct tgttgctctg tggttcgcgg ggctgccgcc
    10741 cgcctgccgg cgggcggcgg agcgcgtgcc catggtcgac gctacgttcc agggcgacga
    10801 gtcttacgag gaggttctgg aaaagttggg cttacctgca catcttgtgc ccgagcaggc
    10861 gagcgtggag ctcgcctacg acaaggctcg cctagagctc ggcaaggaaa ttgtcaagaa
    10921 caggaaatat ttggagataa agcccatcga tcgctctggt tccaaaagat tcggcctccg
    10981 aggggagcga gtgctggatc gtgtacatca tggaatagag cagcaccttc cgccgcttat
    11041 gcgcgctcat gtaggccctt acgcggagga tcgcgagcaa gcagttgccc tatttctcga
    11101 ctggacaaaa caactggcca aaacaaggat gctcgatatt ctctctattg gaagttctca
    11161 gctaactcag agccattttg gcgaagattg gacaggcctt tccaatggtg ggggcgtccc
    11221 catcaacaat ccagcagaat atcgagaagt atgggaagct gcgcgtccca tgctagttcg
    11281 aacctatgca ggcacaaaac atgtgcctga aatggcaaaa atgcatgaag aagcacttga
    11341 tatctgctgg catgcccttt ctttctggtg gttttcgctg ctcgatggtc gtggggagaa
    11401 caccgtcttg cagaatctcg aagagcattt cgaggcattg cgctatatcg cgtccaccga
    11461 taaagccatg gaaccaaatg tgccgcatca gtttgctttt cgtggatgcg atgatgtggg
    11521 ttatatcgtt tctggttatg ttgcagctaa ggcagcgaag gctcagggga ttcgcacttt
    11581 gatccttcaa atcatgctca atactccaaa gtatacctgg ggaatccagg atattgccaa
    11641 ggcgcgtgcc ttaaggaccc ttgtagggga gctagaagga cctgattttg cagtaattct
    11701 gcagcccagg ggtggtctgg actacttttc gtccgatttg gagaaagcca gggctcaact
    11761 cgctgcggtg actatgctta tggatgacat cgagccagat aatcctgcaa gccctcagat
    11821 aattcatgtg gtgagctatt ctgaaggcgt aaggcttgcc gatcctgaag tggtggaaga
    11881 atcggtgcag ataaccaggt atgcgctcaa ggagtatcgc cgcctaaaaa agaaaggtga
    11941 tatgccagaa tatggcaagt ctgcaatggt cgaggctaga acgcggagct tattgaaaga
    12001 ggccaagaac atgatcaaat tcattgaagc tacaattcca aatacctata gtccgcgcgg
    12061 tttttattcg atgctaagat cgggcgtttt tcctctaccc tggcttattg cctgcaaaga
    12121 tgaattcgaa gcggctgtaa atcagcccgt gcagtttctg catggtggcg ttcatgtagt
    12181 ggatagagaa ggcaagcctc tctcagtgag tcagcgactc tttgggatag caagaaatct
    12241 cgaagatatt ggctttgcgc cttctattac acatagtgct gactcaagct ggttggctag
    12301 tgggagtgga ggtgggcaaa aggagcaata tggtgaaaaa cactgattgg gaaggcattg
    12361 taacacacct cgatcatata gccgccaaag gcaagaagat ggtctggtgt gtagtgggag
    12421 caggaaatgg cggtattgca atggcaggcc atctcggcat catgggtttt cctgttcaaa
    12481 tgtataatcg cacagacgag catctcaatg cgattcgctg gtatggaggc atagatatcg
    12541 agggaatggt gactggcttc gggcctgtgc gtctggcaag cgctaatatg ggagatgcac
    12601 ttgaaggttc ggatgttata atgatagtga ctccttcgac tgctcactat aatttagcca
    12661 ccttgatggc accttttctg aaagacggac agattgtagt tctaaatccg ggaaggaccg
    12721 gaggggtact ggaattcagg gcagagctag agcgccagaa atgtgaggct agtgtagtcc
    12781 tggtcgaagc tcagacattc gtctatgcct cgcgtatgat cactcgaaat agagcccata
    12841 tttttagcat caagaatgga gttccagtca gcgctctgcc ttcgtttctt acccccgccg
    12901 tgctaaaagt gatcgatgtt gcatttccac agttcattgc agggagcaat gttctggcaa
    12961 caagcataga gaatatagga gccgtgtttc atccagcgct tacgcttcta aatgctggat
    13021 ggatcgaatc cacaggtgga gatttcgaat attatcttca aggtatttct ccatcagtgg
    13081 caatggtgct tcaagcaatc gacgatgaaa gacttgcgct tgcacgggct ctggggataa
    13141 ggacggtctc cgctagagag tggctgtatc taacctacga ttcagtggga gatgatctgt
    13201 ataccgcgat tcgttctacc ctggcttatg cagggataaa agcgccgcgt tctatcgaac
    13261 accgctatat atgggaagat gtgcccatga gccttgtacc tatggccagt atggccgcaa
    13321 tgtatggtat cgagactccg gcgataaacc ttatcataga tcttgctaat ctgatgctca
    13381 gccaggacta tagagccaat gggcgcacgg tacattctct aggtatcgaa ggcatgtcgg
    13441 tagaagaact ccaccgtctt gtcaccgagg gctgagcggg gctcttaagg gaggtaaata
    13501 taatgaatga aactctaatt gcaataacac aatggatcga caatttgccg tcagggattg
    13561 gggctatcct tattctgtta gttgggtggg caattgcgaa gcttctgaag ctcctggtgc
    13621 caattatatt agcctggctt aaattcgatg ttcttgcgga gaagatcggt atcaaggaat
    13681 ttttgcgcaa aggaaatgtt cgatattcgg cttctaagct cattggcgtc tttctgtatt
    13741 gggttgtaat gctttttgcg ttggcagaag ccgcgtcagt gcttgacaaa agggctgcgg
    13801 agacaatttc ctcctggctc tacggtgcga ttccttcaat tctggccacc ataatcactg
    13861 cgacaatcgg catctttatc gtaaattttc tttcgaactt cgtaatgaca ataatgagca
    13921 atgcgggtct gagttccgcg gccttggtta agagcctcat aagagtggtg ggctatatca
    13981 ttgttataat catggtagtt gaacaacttg gattcgagtc gatcatgaat gtccttcttc
    14041 tattgctaat tggttccgca gcgcttggag tcgcgatagc gattggactt ggttgcaaag
    14101 atatcgccag gcaatatgtg gatagctttc tcaggtctct tcgggagcgt gagcgggctt
    14161 cgcatggaag cgatcttgag ggatagccgg ctgtagattc tttctttatg atgaaaattc
    14221 ctgctcgaga gctgttctca tgttttttat gatgtcatga actgcgaggt ggatgccctt
    14281 cttgtctgct ctgcaaagat ttttggtttc tatgggctca ccgaattcta cgaccacgtg
    14341 gcgatacgga ttgatatgaa aactgcctgg tctcatgact ttttcggttc catatataca
    14401 tataggcaca atgggcacag cacagcgaac cgccgcaaat aatccacccc ggttgaattc
    14461 atctattcca ttgccctggt gcatatgtcc ttcagggaat aatataaggc ttcggccttt
    14521 ctttaatcgc ttttccatgg ctttcagggc acgcacttct gattgcggaa ggccttgatt
    14581 gacaaagaca gagccgcttg cgcgcatcca ataagataga gcaggtattc ttccaaataa
    14641 gtcattagcc acaaaggcgg caggttgggg gatcgatgcg aagagcacgg gtatgtcggc
    14701 aggtgatcgg tgattagaag caaaaagata agtcctgtct ggaaggattt tgtccgcgcc
    14761 ccttacctcg atttccattt ttgtgatctt tattaagctg cgcgaccata aaaagatggt
    14821 ctcaaacaca tactggtcga gttcttcttg ggttttgatt acaaaagtgc gcggaattcg
    14881 acgcatggtt tttgctgttc gcatgagaat gcggggcagc ataaatctga aatcttcttt
    14941 gatagcagcg cgcagaatgg caaaaaacgg cttcttgctc cgggtaacaa tttccgcata
    15001 tcggtttcca tctatttctt tttcgatatc aaaattgtcg gttgtagtag tcgtggaggg
    15061 attgggcata ggtatttcag aattgtaaat tgaatacctt ttatctttga tatctatgtt
    15121 ctgctggtct gccatgcgtt cgctcaatgc ccctaataat agtatctata tttttgacac
    15181 gattagtcaa gaatcaaaat atctgtatag gctttgcgat ttacggctat caatttcttg
    15241 aagatcacat tctctttgct aagactctct tctcataatc ctttacgtct tcgagccaac
    15301 caaggccata agggacattt tgtgtctcgc aataatggtg ccatatggcg ccaaaaggta
    15361 gagagccgga ttcttcgata tatgacagcc ttgttccgaa atcagcttgt tcttctgcgg
    15421 cccgcatagg ttctaagggc tcgagaaaag catagagaag cgctctttgg gtgctgcgtg
    15481 caccattgat ccatgctgaa atacgattta cgctcgaatc gaagtaatcg agagcaagat
    15541 ggactctatc ccagacatgc attctgacaa tctctctgca gacatctgtt agcgaatcat
    15601 tccagatgac tatgtgatct gagtcccagc gcaatcctct gctcaagtgg aggatcagat
    15661 atggtacata gagaagagtc gaagaaattt tgtccgctac gttttcggtg gggtggaagt
    15721 ggcccatatc gtagcataga ccaatggctt tctgagatgc ataacctaga tagaactcgt
    15781 gagaacctgc cacataggct tctgatccta taccgaagag cttggattcg acgctgtcta
    15841 gcactcggtc gtttggcagt ttttcggaat atatggcatc gagagattct ttgagtctta
    15901 aacgcggacc aaggcggtcg gccggcatat ctttgtaccc atcaggtacc cagagattgt
    15961 tgacgacatg atccttgagc gcatcggcga tcgactgggc tatgcgtcta ctcgccttgc
    16021 catgccgaat ccagaaatct cgaatattat tatcggcatg agctagagtc cagccgcttt
    16081 ctgctagagg atgagaaaag aatgtcggat tgaagtcaag gcctatatgc tgttgtttgg
    16141 cccattctat ccaaccttca tagtagctag gttcaagatc gcaacgatct actttagcgc
    16201 cgcctgtctc cgcatacata gcgtgtaggt taaagcgttt cttgccgggg atcaggctga
    16261 aggcgaaggc ggcgtctgag cgcagctctt cggcattgcg cgcccgccca ggatagcttc
    16321 ctgtggagag tatgccgcca gagagtgaag ttgcaccgtc gaagcctatt acatcatctc
    16381 cctgccagca atggatggaa agtggtatag aggcgagggt ctctatggcc tgatcggtat
    16441 cgatggcaag ttcagcataa atctctcgcg ctgctttgta ttcttgctct gttctaggca
    16501 ttgggttctc cttccttcat agccagagat catttcttta gtataggggc actttccgaa
    16561 gaaggagagg tcagcacatt ggcagctact cctgaccgga atttttctac ttcccaacct
    16621 cgaataaaat ctgcatcttc tgattccatc ggatgaacgc ctccaaatga ctgagcgtac
    16681 caggcgattt ttgccgcatc ctggaagagc aggcaggccc gctcggctgc attgtcgtct
    16741 tttcctaggc cgaatatgcc ggttctttgg atggcgagga tttttggtaa ttcgccatgg
    16801 ttggcgatgt attcgtggat tgccgatttg atttgtttga gatcgctggc tgctgattgg
    16861 ctgagaaaaa gaggccaggc tccggcatag acaatgtggt cgggcgtgaa tggttggcta
    16921 agtggttcaa aagcttcggg tgaacccaaa aaatacccca aaagcgaatt taacgtaaag
    16981 gaaaatctta cgttaaattc cgaggatatc gcttctatca tcacccttat atcctccgat
    17041 ggaagctcat tcttgacttt gctcgccaca gaatgagaat catcatccaa aggcaacggt
    17101 ctcacccatt ttcccagagt atcaaaaatg tacctatata gatcatctat ttcatcggca
    17161 ttatccgcgg cgacgaatac tccatgattc cgcaagaaaa gaatcttaat tgggcatctg
    17221 cccatagctt tacgggttct aaacaatgtg cgaactctat ctgccagggt gtatccgggc
    17281 gttgtataag gtagtacttc ggcttcttct ccaaatagct caggtactct cttgagagct
    17341 tcgaccgagc acattagacc atttaccagc gccggatgca gatgcacaac taacctaaat
    17401 gggaataagg catgcagcag tgtttcaaca ctcggccgca tcgtctgtcc cggcagtctt
    17461 gaggcaagaa gcctttcgag cacgatttct tcaggatcgg tagcgtcgga agaaagcgga
    17521 gcttcgagaa tgtttatgag cttaggaagc gataacaata caaagcctct ctcgtcgatt
    17581 gtccccaatg agactcccga agcctttacc gccattacat tatcttgctt tatagagcta
    17641 tttcctccac cagccaacac ataccgggag tccgcgccgt aacggcgaga tagccttata
    17701 atagaatcga gattcatgca caaaccccca tgcgttcagt ccatatggaa catttctttg
    17761 agcgaccata tttttggcga attatccgca ttcgtatcca tgaggtcttt catcgagtcc
    17821 caccacttgc gcattattgg taattcggac aaggagtcgc tcgtctcatc ttcggctagg
    17881 ctttgaattg caaaaagcct taatgtctca ggatctaagt atatcgaata attcgaaatg
    17941 cctgctttcc gcagctcgca ctttagttcg ggccatattt catcatgtct gcgcttatat
    18001 tccgcttcat ttccaggttt gagctgcatg acaaatgcat agcgtttcat tt
//
LOCUS       DAOOWY010000033        16800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3446_length_16800_cov_7.694655, whole genome shotgun sequence.
ACCESSION   DAOOWY010000033 DAOOWY010000000
VERSION     DAOOWY010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16800
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_16800_cov_7.694655"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1480)
                     /locus_tag="PK105_04095"
     CDS             complement(5..1480)
                     /locus_tag="PK105_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU60921.1"
                     /translation="MILADVAAALPGERDFRLVDIFIKGGKIASIKEASIKKRIENPP
                     KIGDPGTNEAADPYDYEDILDAHGLLAFPGAIDPHVHFDEPGFTHREDFMHGSAESAQ
                     GGVTTIIDMPCTSIPPVISDDALEEKLSIVQKKALIDFGFYGGINGNMAPDSIPQVVA
                     SLAPRVLGFKSYFISGMDSFPAVNEAQFVAAAQACGKEGRPLLLHAEDPYVISEATKE
                     RQALRGTQKPQWIDYYASRPMDSEIAAVMNALKLAGQYAKYVHIVHVGTAKAAIAAVG
                     RGATCETCAHYLAFDETDFARFGAALKTAPPVKSPEQKAILWHLLANGKINFVTSDHA
                     GAPDYEKFTDDPLLAYGGIPGTGTLFPYILSEGLFAKRLSLDRFLEVTSGGAARRYGL
                     WQGKGSLAPRKDADIVLVDPYHTRRIDPSAMMSKNAITPFAGMVLSGRIEGTWVRGSC
                     VYASVRLAAALKAPKDFVFSNEEGMILAKPGYGTFLTWGYR"
     gene            complement(1511..3919)
                     /locus_tag="PK105_04100"
     CDS             complement(1511..3919)
                     /locus_tag="PK105_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU60922.1"
                     /translation="MNNKDILELDESLTRKANSDMSQAWAGKVELESGSSGLIPESMP
                     NVLGQTQYIDDITRPAGCLQAVVVLSQSAHGRIRKIHTEEALELDTSVRVILASDIPG
                     TNQIGFNKPDEPLLPESEWDYWGQPLAIVVANSRILARRAASLVRIEGENLPEVIDPR
                     EAAAKGDFIFPPRTIACGDVREAFSRCAFIVEGRVDSGGQEHVYLETQGAIAQVIDGR
                     RVLVISSTQGPTGVQKAVAQVLGLPMSMVEVEARRLGGAFGGKEDQGANWACMAAVAS
                     WVTGKPVEIYLNRRDDMRATGKRHPYSTDFRLGADASGKLIAFEADYYQNSGSTCDLS
                     AAILARTVLHATGAYQIPNVRVTGYMCHTNLPSFTAFRGFGGPQAFFVIESAIDALAQ
                     KMGIDAVEIKRNNLFKEGDVTYYGMTMERVRASDSVERLLCKVDYPALKAKIAEFNAS
                     HRLEKKGIGLIPVAFGISFTKLQMNQAGALVHIYTDGSVVVSTGAIEMGQQVARKIAI
                     IAAKTLGVSIDRVTVQRTTTLTVANTVPTAASTGSDMNGMAARIACLEIRGRLLKKAA
                     ELLMVDLDKVDIKDGAVLLNGAPSHISWEQLVEAMSASRQDLSAHGFYATPTLEYDMK
                     TERGKPFAYHVYGAALAEASIDVLRGTCTIDRVTIVHDIGESIDQSVDIGQIEGALAQ
                     GLGWALLEDLRFEPNGKPISDTLSTYKVPDIGFMPPGIDIEFLPGIDNPFAPYNSKAV
                     GEPPLQYGIAGYFAVLEAIRAATGKALEYYDLPLIPEKITKLLVLEQDNNKRATANRI
                     RTKA"
     gene            complement(3916..5442)
                     /locus_tag="PK105_04105"
     CDS             complement(3916..5442)
                     /locus_tag="PK105_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU60923.1"
                     /translation="MACLRIKVNNTWQDIRSDEGTPAIDIIRGELGLMATKEGCREGD
                     CGACAVLIGEYAAGQLRYLAVPSCLLALGELKGKHLITLEGLIEGTSGGLTPVMRAFL
                     DENASQCGFCTPGFIIALTSWLAEPRLLDLAGALRAIDGNLCRCTGYAAIRRAVRRLI
                     EQFKDLPLEPEARLNALVNAQVLPPSVREFADEASTQVLLNSNNDKENPELTPKQRMT
                     SPGFVILGGGTDYYVKNPEPNDGFSPFLIRMRPEFKEIDYLDENGQRWLEIGAAVTVR
                     EFFASELIRQEVPGIEQFETMFASTLIRNLATIGGNIVNASPVADITAMLLALNASIV
                     IASQIQEGFDLSLTRTCKLEEFFLGYKTIDLKPTEVIKAIRIPSCNDPTARKFSFEKA
                     SKRKNLDIAAVNTAISFRIEEQRFKDVHISLGGVAPTPTVNKAAMELLEGSSCPVSDG
                     KIMAELAKRVASSAEASVRPISDVRGSEEYRRRMVYRLMLAHFVRIFEDSGVAEELFP
                     "
     gene            complement(5606..7336)
                     /gene="ade"
                     /locus_tag="PK105_04110"
     CDS             complement(5606..7336)
                     /gene="ade"
                     /locus_tag="PK105_04110"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOU60924.1"
                     /translation="MKKDIIEAAMGKAQCDVNYTNCILVDVLSGRLLKDSVISIKNGY
                     VAGINDGLSAKETIDLKGMYLAPGLIDAHVHIESSLLTPAEYAKVVIPHGTTTVVADP
                     HEIANVMGYDGMRYMLNVSENIPLDVYFMVPSCVPATDFDTAGAALYASDMHQFLQEP
                     RVLGLGEVMNYPGVLAQDPQLMDKIALFKSNGRPIDGHSPGLSGARLSAYIAAGIGSD
                     HECTTAEEALEKVGKGMFIMLREGSTAKDLRNLLSAVRGENAGRFLLCSDDRHCNDLR
                     DEGHIDYSLRLMLDAGIDPIDAIRIASSNAAGWFGIPAQGAIAPGYKADFILFPSFEF
                     FEIKAVIKNGRIVARDGSLIEDFSAEPIAIRDSVNIKWLTAEDFVIPDKQSPVRVIRV
                     QPNSLLTIHGLERLPSENGALVSDLERDILKIFVIERHTGSGNIGKGFIQGLGLKRGA
                     IGSTISHDSHHMIIAGVDDLSIFKAARHLNKIKGGLVYAVGDQILLDLPLPVAGLMSD
                     KPADFVIEKLSDFDRLFLKEGLTATSPLMTLSFMALPVIPSLKITDKGLVDVEKFEKV
                     SLYVNETVKD"
     gene            complement(7333..8283)
                     /locus_tag="PK105_04115"
     CDS             complement(7333..8283)
                     /locus_tag="PK105_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60925.1"
                     /translation="MDFITSILTITIRAGTSLTIATIGEILTERTGILNLGVEGIMLM
                     GALTAFATVFYSHSIMLGILAAIAIGGILALLHAFLTTTMRANQVVSGLSITLFGTGF
                     SSFLGQRLGPESNNFRLVGLRAERILPFAPNWVKDIPILSAFLNQDIMTYFVYLFIPL
                     AWFFLYKTRPGLWLRSVGEDPYTADAMGIDVTKTRYFYTIIGGMLIALGGAHLSLSYT
                     PGWSENITGGRGWIVIALVIFSMWNPARAVWGSLLFGGINAVQFRLQASGTSVPANFL
                     NMLPYIGTILVLVAITWWEALSKRVGAPAALGSSYMREEK"
     gene            complement(8293..9381)
                     /locus_tag="PK105_04120"
     CDS             complement(8293..9381)
                     /locus_tag="PK105_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU60926.1"
                     /translation="MRLVFEKRKTTSISALILVPMVSFAVSLFLTALLLMIFEADPLK
                     TYTAMAIGAFGSLYGFAETLVKAIPLMMTGLGVAIAFRLKFWNIGAEGQLALGGVAAA
                     GVALFMEQFLPGPLLLVGALLGGLLAGALWAGIPALLKTSLNVDETLVTLMMNYIAIL
                     FSEYLYYGPWRDPKGYGFPGSRMFASNAWLPRIVGRAHIGVLIALFLALVLWFILKRT
                     RWGFELQIIGASQQAARYQGIAVERNIVLAITISGALCGLAGAFEVTGISHRLQQGLS
                     IGYGYTAIIVAWMSQLNPLAVPFVAVALAALSVGGDQVQMTMGLPAAMGTVMQGLILF
                     PMLAGSLFTEYRLMAVKDNINSACRDAT"
     gene            complement(9378..10877)
                     /locus_tag="PK105_04125"
     CDS             complement(9378..10877)
                     /locus_tag="PK105_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU60927.1"
                     /translation="MRRISKSFPGVVANDCIDLKLHKGEVLALLGENGAGKSTLMNIL
                     CGLYRQDQGEIYIDGQKVEIHSPRDAQKYGIGMVHQSFKLVNSMNVLENIILGLRDEP
                     FIPDMKRVRKQLMELASQYHLNIDPDAYIWQLSVGEQQRVEILKLLYRNAEVLILDEP
                     TAVLTPQEAHELNRVIKTMKQEGKSAIFITHKMEEVIEFSDRVMVLQKGRVVAETPTS
                     ETSPKDLARMMVGREVLFQIERKPYNPGKTILELSDIEALGDRGLPALKNISFSLRAG
                     EILGIAGVDGNGQRELAEVITGLRKSTKGTLKIADRDMTNKSPLEIICHGVAHIPQDR
                     ASVGAVGDLSVASNLAMKQYRSRPLAQGIILLPKRIIEFARKLIDKFRIATPSPGTQI
                     KFLSGGNVQKTILAREIGAARSLMVAVYPSRGLDVGATESVRRQLINMRDEGLGILLV
                     SEDLDELLQVSDRIAVMFEGRFMDIFDAEKADIENIGMLMAGMNAEAIR"
     gene            complement(10971..12044)
                     /locus_tag="PK105_04130"
     CDS             complement(10971..12044)
                     /locus_tag="PK105_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU60928.1"
                     /translation="MKKIIIGLFAILMALSFVLPVTAQAKKPLKVAFVYIGPPGDLGW
                     TYEHERGRLALQKHFGNKIETKYVENVPEGPDAERIIRQFAIQGFDVIFTTSFGYMDP
                     TYAVAQEFPNIIFEHCSGYKTAPNMATYFGRIEEARYLTGIIAGRMTKTNKIGYVAAF
                     GIPEVVRGLNGFALGVRSVNPNAKIQVVWTNTWYDPVKEREAAIALLDAGCDIIAQHQ
                     DTTEPQKAAQERGKLSIGYDSDMAKFVGDTVLASAIWNWGTYYIRTIQEVLDGTWKTH
                     SYWGGLKDDIVSLSDLSPLVPANVRKEVENAKKKILGGWSIFTGPIKDQNGKIVYKAG
                     EIIPDDKQLSMDWFVEGVVGKVQ"
     gene            complement(12283..13035)
                     /locus_tag="PK105_04135"
     CDS             complement(12283..13035)
                     /locus_tag="PK105_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU60929.1"
                     /translation="MSDHSRKLLWIAVAISGFALIVLLAVFIIFPPNQSSESRPFDLT
                     GRSPAKEISPNDFSAFPSIVVQSSEEEAQVSDTSKNGKTEPQKPESTVIIVPAPSSNQ
                     KTIAAPLQPSGSQSENATAAPKTSQTSQSAQTATQSSTKTSPATTPSASSTQKTTTQP
                     APSSTPKTNSSSMEYWIQVGSFSQQATAEKLRNEYMTRGMTAILSQKDVGGKTYYQVK
                     VGPYASSEEAKKWMLTVKSVKGASQDAFVTSR"
     gene            complement(13073..13696)
                     /gene="coaE"
                     /locus_tag="PK105_04140"
     CDS             complement(13073..13696)
                     /gene="coaE"
                     /locus_tag="PK105_04140"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOU60930.1"
                     /translation="MFILGLTGGYCAGKNEVAIILEKCGWRLTDVDELGHAALPLVCD
                     ELVDAFGSSILKEDGSIDRKRLGSLVFNDTEQLKKLENIVHPAMLSLLDKEIEKAQQN
                     IIEKLCINAALLYRFPQLDSCDAIIEVRAPIYLRIARAMQRDHLSFDEALHRISSQKD
                     LWRKRSSICVPIYMLWNLQSLDELEADTLQLLKRIEDEIKQKRSFIE"
     gene            complement(13714..16569)
                     /gene="polA"
                     /locus_tag="PK105_04145"
     CDS             complement(13714..16569)
                     /gene="polA"
                     /locus_tag="PK105_04145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOU60931.1"
                     /translation="MDNKSPLYILDVYAIIYRSYFAFIRKPLRNSKGENVSALFGFFR
                     FLFSLFEQRKPELFAAALDSIGPTFRHTQFATYKATRQKTPEDLTAQIPRIETILQAL
                     EVPAIRSEGYEADDIIATLADQCRKERRPCFIVSGDKDLLQLVGDDILALRPSENFTF
                     RELDAQGVFDEWGVRPDQIRDFLALTGDASDNVPGVKGIGEKTAQKLISMFGSLEAIY
                     DNLPAIEPESLRKKLEEGRESAFLSRELISLCYDVPNCPHDVAVLKPSLNIEAAAPLL
                     LREDIKSLIPDELRKRLHAKPIAGEEVASAESLQRISGQVRVAPSTNSAISLLAGSPG
                     AIQQASSMAPLESISGLSIGNPMQVKYEPVLSIERLDRIIDEAIARSVCAFDTETDSL
                     DVHKAGLVGFSLAFDADSAWYVPLKSPDAKCIPKDLALSSLSRLMKEQNMLLVGHNLK
                     FDINVLSTAGLEANLPCFDTMIAAWVLDAEAPSFSLSSVASRFLGISGLEYGDVVPKG
                     LTFADVPLATATQYASEDSHFSFLLYQMLSKEIETQSLHNIFYNIEMPLLPILAEMER
                     IGIRVDAEHLRAYGEELQIELSNIESQIFHLVGHEFNLASPKQLADVLFSERKLPVQK
                     RTKTGFSTDTSVLEELAPLDPVPQLILRQRLLTKLKTTYIEPLAELAMAQGRIHTTFM
                     QTGAATGRLSSRDPNLQNIPIREEEGRKIREAFIAEQGHLLISADYSQIELAVMAHLS
                     KDPNLIKAFKEGVDIHKRTASFIFGISEDQVDAERRRIAKTINFGVIYGMSAFRLARD
                     LGISNSLAKQFIDSYFSTYSGVADFIQKTIKEAQERGMTYTLFGRRRKILGINSKNKT
                     EQQAAQRVAVNTPIQGTAADIVKIAMIRVHRRLKKEMPEVHMLLQVHDELVFEAPKAL
                     VEGAMKLIKEEMEHAVLLDIPLRVSMESAASWGQMHL"
     gene            complement(16591..>16800)
                     /locus_tag="PK105_04150"
     CDS             complement(16591..>16800)
                     /locus_tag="PK105_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOU60932.1"
                     /translation="EAYRFLGLEPYAPFAQVKMRYKKLLKEHHPDRHNKSDSDTAEAN
                     AISAKINAAYQLIETWEESQAKSSK"
BASE COUNT         4251 a         4078 c         3792 g         4679 t
ORIGIN      
        1 ttgctcatcg atacccccaa gtaagaaatg tgccataacc aggtttggca agaatcatgc
       61 cttcttcatt agaaaataca aaatctttag gggcctttag tgctgccgca agtctaaccg
      121 aagcatatac gcaagaacct cgaacccatg tgccttcgat tcgtccggag agcaccatcc
      181 cggcaaaagg agttattgca ttcttactca tcattgccga aggatcgatc cgccttgtat
      241 gatatggatc gacaagtaca atatcggcgt ctttgcgggg agccagcgat cctttccctt
      301 gccatagtcc ataccttctt gctgcgcctc cgcttgttac ttcaaggaag cgatcgagcg
      361 agagtctctt agcaaacaag ccctcagata gaatatatgg aaataatgtg ccggtgccag
      421 gaatcccgcc atacgccaaa agcgggtcat ctgtgaattt ttcgtaatct ggcgctcctg
      481 catgatcaga tgtgacaaag ttgatttttc cattggcgag aaggtgccat agaattgctt
      541 tttgttccgg gcttttcacc ggtggagccg tcttgagagc agctccaaag cgagcaaagt
      601 ccgtctcatc gaatgccagg taatgagcac aggtttcaca tgtggcaccg cgccccacgg
      661 ccgcaattgc tgctttagca gtgccgacat gtacaatatg aacatatttc gcatattgtc
      721 ccgctagttt tagtgcattc atcacagcgg ctatttcgga atccatggga cgagaagcat
      781 agtagtctat ccattggggt ttttgtgtac cgcgtaaggc ttgcctttct ttggtagctt
      841 ccgagattac ataaggatcc tctgcatgca aaagaagagg ccgcccttct tttccgcaag
      901 cctgcgcggc ggcgacgaat tgagcttcgt ttacggcagg gaatgaatcc atgcctgata
      961 tgaaatatga tttaaaacca agtacccgag gagccagcga tgctaccacc tgaggaatag
     1021 aatcaggagc catgtttcca ttgataccac catagaagcc aaaatcgatc agcgcttttt
     1081 tttgtacgat tgatagcttt tcttcgagtg catcatcaga gatgacgggt ggaatggaag
     1141 tgcagggcat atctattatg gtagtcacac ctccttgcgc ggattccgca gagccatgca
     1201 taaagtcttc gcgatgtgta aagccgggct catcaaaatg aacatgggga tctattgcac
     1261 ctggaaatgc gagaagccca tgagcgtcta ggatgtcttc ataatcataa ggatcagctg
     1321 cttcatttgt cccaggatct ccgatttttg gcggattttc tattcttttc ttgatgcttg
     1381 cttccttgat agaagctatt ttgccgcctt ttatgaaaat atcgacaagt cgaaaatcgc
     1441 gttcaccagg caatgccgcg gctacatctg cgagtatcat tctgcttctc cctgtcaaaa
     1501 atattccgca ttaggctttt gtcctgatgc ggtttgcggt tgccctctta ttattgtctt
     1561 gctccaatac gagcaactta gttattttct ctgggataag aggcagatcg taatattcga
     1621 gcgcttttcc agttgcggct ctaatggcct cgagaactgc aaaatagcct gctataccat
     1681 attgcagagg gggctcgccg actgcctttg agttatatgg tgcaaaggga ttatcgatgc
     1741 ccggtaggaa ttcgatatcg ataccaggag gcataaagcc gatatccggc actttatagg
     1801 tagataatgt gtccgaaatt ggcttgccat tgggttcgaa gcgcaaatcc tcgagcagag
     1861 cccagcctag cccttgtgca agggctcctt cgatctgacc tatatcgacg ctttgatcga
     1921 tactctctcc aatatcgtgt acaattgtaa ccctgtctat tgtacatgtg ccccgcagga
     1981 catcaatgct ggcctctgct agcgctgcgc catatacatg ataggcaaag ggttttccac
     2041 gctcggtttt catatcatat tcgagtgtcg gcgttgcata aaagccatgt gcggataggt
     2101 cttgacgcga tgcgctcatg gcttcgacaa gttgctccca gctaatatgg ctgggtgctc
     2161 catttagtag tactgctcca tctttgatat ccaccttgtc cagatcgacc ataaggagct
     2221 cagcggcttt cttcaatagt cttcctcgga tttccaagca tgcaattctt gccgccatgc
     2281 cattcatgtc cgatcccgta ctcgcggcgg tcggcacagt attggctact gtcaaagttg
     2341 tagtgcgttg aaccgtaact cgatctatcg aaacaccaag agtttttgcg gcaattatcg
     2401 ctatttttcg agccacctgt tggcccatct caatagcccc tgtcgagacc actaccgatc
     2461 catcggtgta gatatgcact agagctcccg cttgattcat ctgtagcttg gtaaaactta
     2521 tgccaaatgc tacaggaata aggccaatac ctttcttctc aagtcgatga gaggcattga
     2581 attcggcaat tttggccttt agcgcaggat aatcgacctt gcagagaagc cgctcgaccg
     2641 agtcagacgc ccgcactcgc tccatagtca taccataata ggttacatcc ccttctttga
     2701 agagattgtt ccgctttatt tcgacagcat cgattcccat cttctgtgca agcgcatcga
     2761 tagcagattc gatcacgaag aatgcctggg gccctccaaa acctctgaac gccgtgaaag
     2821 agggcaagtt cgtatggcac atatagccgg ttacccgcac atttggaatc tgatatgcac
     2881 cggttgcatg cagcacagtt cgagcaagaa ttgctgccga tagatcgcag gtcgagccag
     2941 aattctggta atagtcggcc tcgaaagcga taagtttgcc actcgcatcg gcgccgagac
     3001 gaaaatccgt agaataagga tgtcgtttgc ctgtagcccg catatcatcc ctgcggttga
     3061 ggtatatttc gactggcttg ccggtgaccc aagaagcaac agcggccatg caagcccaat
     3121 ttgctccctg atcttccttg ccgccaaatg ctccaccgag gcgccttgcc tcgacttcta
     3181 ccatgctcat aggcaggcct agcacttggg caacagcctt ctggacgcct gtcggaccct
     3241 gtgtactcga aatgacaagc actctacgac catctatcac ctgagcaatt gctccctgag
     3301 tttcgaggta aacatgttct tggccgccag aatcgactct gccttcgacg ataaaggcgc
     3361 atctcgaaaa tgcttcgcgc acatcgccac aggctattgt tcttggcgga aagatgaaat
     3421 ctcctttggc agccgcttcc cttggatcta taacttcggg aaggttttct ccctctatgc
     3481 gcacaaggct tgccgctctt cttgcaagga ttcgagaatt tgccaccaca atcgccaaag
     3541 gctggcccca atagtcccac tcactctctg gaaggagcgg ctcgtcgggc ttattgaagc
     3601 caatctgatt agttccgggg atgtcacttg ccaggatgac ccgtacagaa gtatcgagct
     3661 cgagagcttc ttctgtatgg atcttgcgaa ttctaccatg tgcgctctga gatagaacca
     3721 caacggcctg gagacaaccc gctgggcgcg taatatcatc tatgtattga gtttgtccta
     3781 gaacgtttgg cattgattcg ggtatcaagc ccgacgagcc gctttccaat tctactttgc
     3841 ctgcccaggc ttggctcatg tccgaatttg ctttccgggt cagagattca tctagctcaa
     3901 gtatatcttt attgttcatg gaaatagctc ctctgcgaca ccgctatctt caaagatgcg
     3961 cacaaaatgt gcaagcatga gcctgtagac cattctgcgc cgatattctt cagatccgcg
     4021 cacatcactt atgggcctta ccgaagcctc tgccgagctt gcaactctct tagcaagctc
     4081 agccattatc ttcccatccg agacagggca ggatgagcct tcgagtagct ccattgctgc
     4141 cttattgact gttggtgtgg gcgctactcc tccaaggctt atgtgtacat ccttaaacct
     4201 ttgctcttcg attctaaaag atatggccgt attgacggca gcgatatcga gattcttgcg
     4261 cttgctggcc ttttcaaagg aaaatttccg cgcagtcgga tcgttacagg atggaattct
     4321 gattgctttg atgacttccg tgggttttag atctattgtc ttatatccca aaaagaattc
     4381 ttccagctta caagtgcgtg tcaaagacag gtcaaagccc tcttgtatct gagacgcaat
     4441 gactatgctt gcattgagag ctagcagcat tgccgttata tccgctactg gcgaggcatt
     4501 tacgatattt ccgccaatgg tggcgagatt gcgtatcagt gtgcttgcaa acatcgtttc
     4561 gaattgctcg attcctggaa cttcttgtct aataagctct gaagcgaaaa attccctcac
     4621 cgtgacggcg gcgccgattt cgagccatct ttgtccattc tcatctaagt aatcaatctc
     4681 tttgaattct ggcctcattc taatcaagaa tggcgaaaag ccatcgtttg gttcggggtt
     4741 tttcacataa tagtctgtcc ctccacctaa gataacaaag ccaggagatg tcattctctg
     4801 cttgggcgtt aattccggat tctccttatc attattagaa ttcaggagga cttgagtcga
     4861 tgcttcatcg gcaaattcgc ggactgacgg tggaagcact tgagcattca ccagtgcatt
     4921 caatctagcc tcaggctcaa ggggcaggtc tttgaattgc tcgataagcc tgcggactgc
     4981 tcttcgaatc gccgcatacc ccgtacatct gcacaaattc ccatctatgg ctcgcagtgc
     5041 tccagcaaga tccaatagcc ttggctctgc caaccacgaa gtcaaggcga tgatgaatcc
     5101 gggcgtgcaa aatccgcatt ggctggcatt ctcatccaga aacgcccgca taaccggagt
     5161 aagacctccc gaagtccctt cgattaggcc ttcgagggtt ataagatgtt tgcctttgag
     5221 ttcgccaaga gcgagcaagc acgaagggac tgctagatat cgcagttggc cggctgcata
     5281 ttctccgatc aataccgcgc atgcgccaca atcgccttct cgacagcctt cctttgttgc
     5341 cataagtcca agttcgcctc gaattatgtc gattgccggc gtgccttcgt ctgagcgaat
     5401 atcctgccat gtgttgttga cctttattcg caaacaagcc attttactct acctcttcaa
     5461 aaattatacc atagattttt gagataggca tttattacgg cggactcggc tctctgcatg
     5521 cctttattca tggaaatatg caagcttgtg gaaagaactt gccttatata taaggatata
     5581 aggctgccaa gatgccgaat cggtgctaat ctttgactgt ttcgttcacg tagagcgata
     5641 ctttttcgaa cttctcgaca tccacaagac ctttatctgt gattttcagg cttgggatta
     5701 ctggaagagc cataaaggag agagtcataa ggggagaagt tgctgtcagg ccttccttca
     5761 ggaatagtct atcgaaatcg ctcagctttt ctatcacaaa gtctgctggc ttgtcgctca
     5821 taagccctgc aacaggtaag ggcagatcca gcaaaatctg gtctcccacg gcatagacca
     5881 ggccgccctt gattttattg agatgcctgg ctgccttaaa gatcgataaa tcatcgacac
     5941 cagcaatgat catatgatgc gaatcatggc ttatggtgct gcctatcgct ccgcgtttaa
     6001 gcccgagtcc ttgtataaag cctttaccta tgtttcccga gcccgtgtgc ctctctataa
     6061 caaatatctt caggatatcg cgctctaagt ccgaaaccag tgccccattt tccgaaggta
     6121 gtcgctccaa accatgtatg gtcagaagcg aatttggctg gactctgatt actcttaccg
     6181 gagattgctt atccggaatc acgaagtctt ccgcagtcaa ccatttgatg ttgacagagt
     6241 ctctgattgc tattggctct gccgaaaaat cttcgatcag tgaaccatct cttgctacaa
     6301 ttcttccatt cttgatgact gccttgatct cgaagaattc aaatgaaggg aataagataa
     6361 aatcggcctt ataacctggt gcaattgctc cttgagcagg gattccaaac catcctgccg
     6421 cattactcga agctatgcga attgcatcta tgggatcgat acccgcgtct agcataagcc
     6481 ttagcgagta gtctatgtgg ccctcatcgc gtagatcatt gcaatgcctg tcatcggaac
     6541 agagcaggaa gcgccccgca ttctcgcctc gcaccgcgga taacagatta cgcagatctt
     6601 ttgccgtgga accttccctc aacattatga acattccctt gcccactttt tccaatgcct
     6661 cttctgcggt ggtgcactca tggtcagagc ctatgcctgc tgctatgtag gccgatagac
     6721 gagcgccaga tagtccgggt gaatgaccat cgataggtct tccattcgac ttgaacagtg
     6781 cgatcttatc cataagttgt ggatcttggg ccaaaactcc tgggtagttc atcacttcac
     6841 caaggccgag cacgcgaggt tcctgcaaaa actggtgcat atcagaggca tagagcgccg
     6901 cgcccgcagt atcgaaatcg gtggcaggta cgcaggatgg caccataaaa tacacatcga
     6961 gggggatatt ctctgaaaca ttcagcatat atcgcatgcc atcatatccc atcacattgg
     7021 caatctcatg aggatcggca actacagttg tggttccatg aggtattacg accttggcat
     7081 attccgccgg agtcagaagc gatgattcta tatgtacatg ggcatcgatg agaccgggtg
     7141 caagatacat tcctttcaga tctatagttt cctttgccga aagcccatcg ttgattcctg
     7201 ccacataacc attttttatc gaaataacag aatctttgag taaacggccg gaaaggacat
     7261 ctaccaatat acagttggta taattaacgt cgcattgtgc ctttcccatc gcggcctcga
     7321 ttatgtcctt tttcattttt cctccctcat gtatgaggat ccaagcgcgg cgggcgctcc
     7381 gactctcttg gataaggctt cccaccatgt gatagcgacg agaaccagta tggtgccaat
     7441 atatggcaac atgttgagaa agtttgcagg tacgctcgtt ccggatgctt gaagacgaaa
     7501 ctggacggca ttgatccctc caaacaacaa agatccccac acggcgcgtg cgggattcca
     7561 catcgaaaag ataacgagtg caattacgat ccatcctcgc ccgccggtaa tgttctccga
     7621 ccatcctggt gtgtaggaaa gagaaagatg agcaccacct agggctatca gcatgcctcc
     7681 gattattgta tagaagtacc tggtctttgt tacatcgatt cccatcgcat cagcggtata
     7741 gggatcttcc cccacagaac gaagccataa gcccggtctt gtcttgtaaa ggaaaaacca
     7801 tgcaagggga atgaaaagat atacaaaata ggtcattata tcttgattca aaaaagccga
     7861 aagaatcgga atgtccttta cccaattcgg agcaaagggt aggattcgct ctgctctaag
     7921 gcctacaagt ctgaaattgt tggattccgg tccaagcctt tgtccaagaa atgacgaaaa
     7981 tcctgttcca aatagcgtta tcgaaagccc tgagacgacc tgatttgccc tcatcgttgt
     8041 ggtaagaaaa gcatgcagca aggcgagaat gcctccgatc gcaattgctg ctaaaattcc
     8101 cagcatgatg ctgtggctat aaaaaacggt ggcaaaagct gttaaagccc ccataagcat
     8161 gattccctcg acaccgagat tcagaatgcc cgttcgttct gtgagtattt ctccgatagt
     8221 tgcaatggta aggcttgttc ctgctcgaat ggtaatcgtt agaattgaag tgataaaatc
     8281 catgcattgc tcctaagttg catctcggca ggctgaattt atgttgtctt ttactgccat
     8341 aagtctgtat tcggtgaata aggatcccgc aagcatcgga aatagtatga gaccttgcat
     8401 cactgtaccc atggctgcgg gtagccccat tgtcatctgc acctgatcgc ctccaacaga
     8461 tagtgcggcg agtgctaccg ctacaaaagg aacagcaaga ggattgagtt ggctcatcca
     8521 agcaactatt attgctgtat atccgtaacc tattgaaagg ccttgttgaa ggcggtgcga
     8581 tatacctgtt acctcgaatg ctccagcgag tccgcagaga gccccagaga ttgtaattgc
     8641 aagaacaata ttgcgttcaa cggcgattcc ctgatatctt gccgcttgct ggcttgcacc
     8701 gatgatctgt agctcgaagc cccaacgtgt gcgctttaga ataaaccata gaacaagcgc
     8761 aaggaacaag gcaatcaaaa cgccaatatg cgcgcgtccc actatgcgcg gaagccatgc
     8821 attggatgca aacatacgcg aaccagggaa tccatatccc ttaggatcac gccatggacc
     8881 atagtacagg tattcggaga acagaatagc tatataattc atcataagag taacaagcgt
     8941 ctcatcgaca ttgagagaag tctttagaag agctggtata ccagcccaaa gcgcaccggc
     9001 aagcaagcct cctaataggg cgcccactaa gagtaaagga cccggcagaa attgttccat
     9061 aaaaagagct actcccgctg ccgctacgcc tcccaatgca agctgacctt cggctcctat
     9121 attccagaac ttcaaccgaa aagcgattgc cactccaaga cctgtcatca ttagaggaat
     9181 agcctttaca agtgtctctg caaaaccata caaagatcca aaagcaccaa ttgccattgc
     9241 tgtataggtt tttaaagggt cggcttcaaa gatcataagc aacagggcag tcagaaacag
     9301 agagaccgca aatgatacca taggcacgag tataagagcg gaaatcgatg ttgtttttct
     9361 tttttcgaat acaagtctca tctgatggcc tccgcattca tgcccgccat aagcatccca
     9421 atattttcga tatctgcttt ttcagcgtcg aaaatatcca taaaccttcc ttcgaacatt
     9481 actgctatgc gatccgatac ttgcaggagt tcatccagat cttcggatac aagcagaatt
     9541 cccaggcctt catctcgcat attgatcagt tgacgcctta ctgattctgt agcccccaca
     9601 tcgagccctc gcgaaggata gacagcaacc atcaaagatc gtgctgcgcc gatctccctt
     9661 gccaaaatcg tcttttgcac atttccgccc gagagaaact tgatctgagt gccgggcgat
     9721 ggagtggcga tgcgaaattt gtctatcaat ttgcgggcaa attcgatgat tcttttggga
     9781 aggaggataa ttccctgtgc gagaggcctg gaacgatatt gcttcatagc aagattcgaa
     9841 gccacgctga gatctcctac agctcctacg gatgccctat cttgaggaat atgggctact
     9901 ccatgacaaa tgatctcaag tggagacttg ttggtcatat ctctgtctgc aatctttagc
     9961 gttccttttg tggactttcg cagtccggtt atgacttccg ccagctctcg ttggccattg
    10021 ccatcgaccc ctgctatgcc gagtatttct cctgccctaa ggctaaatga tatattcttg
    10081 agggcaggca gtcctctatc gccaagcgct tcaatatcgg aaagctccag tattgtcttg
    10141 cccgggttgt agggctttct ttctatttga aaaagaactt cacgtcccac catcattcgg
    10201 gctaagtctt taggactcgt ctccgaagta ggtgtttccg caacaactct ccctttctgc
    10261 aagaccatga cgcgatccga gaactcaata acttcttcca ttttgtgagt tatgaatatt
    10321 gcggatttgc cctcctgctt catggtcttt atgacgcgat tcagttcatg agcctcttgt
    10381 ggagtgagga ccgcggtggg ttcatccaga atcaggactt cggcatttct gtagagaagt
    10441 tttaggatct cgactctctg ctgttcccca acagatagct gccagatata ggcatctggg
    10501 tcgatattga gatgatattg agatgctaat tccattagct gcttgcggac tcgtttcata
    10561 tccggaatga atggctcatc gcggaggcct aggatgatgt tctcaagaac attcatcgaa
    10621 tttacaagct tgaaactctg atgcaccatc cctattccat atttctgagc atctcgagga
    10681 gagtggatct cgactttctg tccatctatg tatatttcac cttgatcttg cctatacaag
    10741 ccacacagta tattcatcaa ggtgcttttt ccagctccat tctctccaag aagcgctaag
    10801 acttctccct tatggagttt caaatcgatg caatcatttg caacgactcc ggggaaggac
    10861 ttggagatcc gtcgcatttc gattctcgga atgttcgaat caatcataga ttttatggat
    10921 agagaaagcc ggtatgtatt gcatagagca cacataccgg caataaagaa tcactgaacc
    10981 ttacctacta cgccttctac aaaccaatcc atcgagagtt gcttgtcatc tggaatgatt
    11041 tcgccggcct tatagacaat tttgccattt tgatccttta tggggccggt aaagatgctc
    11101 cagccgccca atatcttctt ctttgcattc tccacttctt ttctaacatt ggctggcacg
    11161 agcggactca gatcggagag gctgactata tcatctttta atccacccca gtaggaatgt
    11221 gtcttccagg ttccgtccaa tacttcttgg atagtcctta tatagtaggt tccccagttc
    11281 caaattgcac tggcaagaac agtatctcct acgaattttg ccatatctga gtcatagcca
    11341 atagatagct tacctctctc ctgtgctgct ttctggggtt ccgtagtatc ctggtgttgt
    11401 gcaatgatat cgcagcctgc atcgagaagt gctattgcgg cttctctttc ttttactgga
    11461 tcataccagg tatttgtcca gacgacctgg atcttggcat taggattgac agagcgtact
    11521 cccagagcga agccatttaa accccttaca acttcaggaa ttccaaaggc tgctacatat
    11581 ccgatcttat tggtcttggt catgcggcca gcaataatgc cagtcagata gcgagcttct
    11641 tcgattcgac caaaatatgt tgccatattt ggagctgtct tatatcccga gcagtgttcg
    11701 aaaattatat ttgggaattc ctgagccacc gcatatgttg gatccatgta accaaagctt
    11761 gtagtgaaaa taacatcgaa tccctgaatg gcgaattggc gaatgatccg ttctgcatcc
    11821 gggccttccg gaacgttttc tacatatttg gtttcgattt tattgccaaa atgcttctgt
    11881 aaagcgagcc gtcctcgttc atgctcatag gtccaaccga gatcaccagg tgggccgata
    11941 taaacaaatg cgacctttag aggttttttt gcctgagcag tgactggcag cacgaaggaa
    12001 agtgccataa gaatagcaaa caggccgata atgatctttt tcattggcga ctcctttatt
    12061 tgattgctaa aaacggaaaa atccgctggc ataaagcacc taaaaaagtt tagcttccaa
    12121 atcgcctgtt gtcaaatgaa atttcgggct cgattgcctt agggagaatt attatgcatt
    12181 ctcaggttct ttaatttatg agggttgaga gaaagaattg tcatttatga ttattgttat
    12241 atgttagatt ttgagggttg aattattttg cggatttaat gcttatcgac ttgttacaaa
    12301 agcatcctga cttgcgcctt ttacggattt taccgtaagc atccattttt ttgcctcttc
    12361 cgatgaagca taaggcccga ccttgacttg ataataggtt ttaccgccaa catctttctg
    12421 cgaaaggatc gctgtcattc ctcgagtcat atattcattt cgaagttttt ctgcagtagc
    12481 ttgttgcgaa aaactaccaa cctgaatcca atattccatg ctcgatgagt tggtttttgg
    12541 tgtggaagag ggtgctggct gggttgttgt cttctgtgtc gatgaggcgc ttggcgttgt
    12601 tgccgggctt gtctttgtag agctctgagt tgctgtctgg gcagattgag atgtctgaga
    12661 tgtcttaggt gcagcagtgg cattttcaga ttgcgaccca cttggttgta aaggagcggc
    12721 aatggtcttt tgatttgacg aaggagcggg aactatgata acagtgctct ccggtttttg
    12781 gggctctgtt tttccattct ttgaagtatc tgagacctga gcctcttctt ctgaggactg
    12841 aacgacgata cttggaaagg cggaaaagtc atttggggat atctctttgg caggcgacct
    12901 gcccgtcagg tcaaaaggcc tggattcaga tgactgattg ggtggaaaga taatgaatac
    12961 agcaagaagg acaatcaacg cgaagccact tatcgcgacg gcaatccata gcaattttct
    13021 tgaatgatcc gacatttcta gacccctatt taaataatat cggcaggatg aattattcga
    13081 taaagcttcg cttttgtttt atttcatctt ctattctctt gagcaactgc agagtatcgg
    13141 cctcgagttc atccagagac tggagattcc agagcatata tatagggacg cagatgctag
    13201 atctctttct ccaaaggtct ttttggcttg agattctatg aagagcttca tcgaaggaaa
    13261 gatgatctct ctgcatcgcc ctagcgattc gtaaataaat cggtgcacgc acttcaatga
    13321 ttgcatcgca tgaatcaagc tgtggaaaac gatagagcaa tgccgcattt atgcatagtt
    13381 tttctattat attctgctgt gctttctcta tttccttatc gagcagagag agcattgctg
    13441 gatgcacgat gttttcaagc tttttcaatt gctctgtatc attgaatacc agcgatccaa
    13501 gtctttttcg atctatggag ccatcttctt tgagtatcga gctaccaaaa gcatcgacaa
    13561 gttcatcgca taccagagga agcgccgcat ggccgagctc atcgacatca gtcagcctcc
    13621 agccacattt ttccagtatg attgcgactt catttttccc agcgcaatag cctcccgtta
    13681 ggccaagtat gaacatcgat ctttcctttt agattaaaga tgcatttggc cccaagatgc
    13741 ggccgattcc atgcttactc taagtggaat atcgagcaat acagcatgtt ccatttcctc
    13801 tttgatcagc ttcatggctc cttccaccag ggctttcggc gcttcaaaga caagctcatc
    13861 gtgcacctgt agcagcatat gcacctcggg catttccttc tttagccttc gatgcactct
    13921 tatcatcgct atctttacta tgtctgcggc tgtcccttga attggggtat tgacggcaac
    13981 tctctgtgcc gcctgctgct cggttttgtt ctttgaattg atgccgagaa ttttgcgtct
    14041 gcgcccgaac agagtataag tcatccctct ttcttgagct tccttgatcg tcttttgaat
    14101 aaaatccgct acacctgaat aggtggaaaa atatgaatcg atgaattgct ttgcaaggct
    14161 attcgaaata cccagatctc gtgccagcct gaatgcgctc atgccataga tgaccccgaa
    14221 attgatggtc ttggcaattc ttctgcgttc tgcgtcgacc tggtcttcgg atatcccaaa
    14281 gataaaggaa gcggttcgct tatggatatc gactccctct ttgaatgcct ttataagatt
    14341 aggatctttg gagagatgag ccatgactgc cagctcgatt tgagaataat cggcagagat
    14401 aagcagatgc ccctgctcgg caataaaggc ctctcgtatt tttctgcctt cttcttcgcg
    14461 tatggggata ttctggaggt ttggatctct gctggaaagc cttccggttg ctgcaccggt
    14521 ctgcataaag gtagtgtgaa ttcgtccttg ggccatcgcc aattctgcaa gaggttcaat
    14581 atatgtcgtc tttagtttgg tcaatagcct ttgtctaaga atgagctgag gaaccggatc
    14641 caggggtgca agctcttcca gcactgaagt atcggtggaa aagcctgttt ttgttcgctt
    14701 ttgtactggc agtttccgct cagaaaatag aacatcagcc agctgctttg gagaggctaa
    14761 attgaattca tggccaacca gatgaaaaat ctggctttcg atattcgaaa gctctatttg
    14821 cagctcttct ccataggcac gaaggtgctc tgcatcgaca cgaatcccta tgcgttccat
    14881 ctccgcaagg attggcaata aaggcatctc tatattatag aatatattat gaagactctg
    14941 cgtctcaatt tccttagaga gcatttgata gagaagaaaa gaaaagtggg aatcttccga
    15001 tgcgtattga gttgcggtgg ccaaaggcac atcagcaaag gtaaggcctt tcggtactac
    15061 atccccatat tctagtccag aaatcccgag aaaacggcta gcaaccgaag atagcgaaaa
    15121 cgaaggagct tcagcatcca gaacccatgc ggctatcatc gtgtcaaagc agggcaggtt
    15181 tgcttccaga cctgcagtgg agagcacatt tatatcgaac ttcagattgt ggccaacaag
    15241 gagcatgttt tgctccttca tgagtctgga gagggaagaa agggcaagat cctttggaat
    15301 gcattttgca tcaggactct ttaagggaac gtaccaggct gaatcagcat cgaaagcaag
    15361 agaaaaaccg acaagtcctg ctttgtgcac atctagagaa tcggtctcgg tgtcaaaagc
    15421 gcagacagat cgagcaatgg cctcatcgat aatcctatcg agtctttcga tcgatagcac
    15481 tggttcgtat ttcacttgca ttggattacc gatcgataat cctgaaatac tctcaagagg
    15541 cgccatggaa gaagcctgtt ggattgctcc aggagatccg gccaaaagag aaattgcaga
    15601 attcgtagaa ggcgcaaccc gaacttgacc tgaaattctc tgcaaactct cggccgaagc
    15661 gacttcttct ccagctatgg gttttgcgtg taatcgcttt cgcaattcat cggggataag
    15721 gcttttgatg tcttctctta aaagaagagg agctgccgct tcgatattca atgaaggttt
    15781 caaaacagcg acgtcatgcg ggcaattagg aacatcatag cacagagaaa taagctctct
    15841 agataaaaat gcgctctccc ttccttcttc cagctttttg cgtagagatt ctggctctat
    15901 cgcaggcaaa ttatcatata tggcttcgag tgaaccgaac atcgaaatca gcttctgagc
    15961 cgttttttct cctataccct ttacccccgg cacattatcc gaagcatctc ctgtaagagc
    16021 aaggaaatcg cggatttgat cgggtcttac cccccactca tcgaagactc cttgggcatc
    16081 aagctctcga aatgtaaagt tttccgaagg gcgaagcgcc aatatatcat ctcctacaag
    16141 ttgcagtaga tccttatccc cggaaacaat aaaacatggt cgacgctctt tccgacattg
    16201 gtctgccaag gttgctatga tatcgtcggc ctcatatcct tcagatcgaa tcgctggcac
    16261 ttccaatgcc tgaaggatcg tctcgattct tggaatctgt gcggtcagat cttccggtgt
    16321 tttttggcga gttgccttat aggtggcaaa ttgcgtatgg cgaaatgtgg gtcctatgga
    16381 atctagggca gcagcaaata gttctggttt cctttgctcg aatagagaaa aaagaaaacg
    16441 gaaaaaacca aataatgctg atacattttc acccttagag tttcgcagag gctttcggat
    16501 aaaagcaaaa taggaacgat agataattgc ataaacatcg aggatgtata aaggggattt
    16561 attatccata ttgttcttcc atatgtattc ctatttggag ctcttagctt gagactcttc
    16621 ccatgtttcg atgagctgat aggccgcgtt gatcttagcg cttatcgcat ttgcttccgc
    16681 ggtatctgaa tcggatttgt tatgtcgatc cggatgatgc tcttttaaga gttttttata
    16741 tcgcatcttt acctgcgcaa aaggagcata gggttctaat cctagaaatc tgtaagcctc
//
LOCUS       DAOOWY010000034        16441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3557_length_16441_cov_8.430245, whole genome shotgun sequence.
ACCESSION   DAOOWY010000034 DAOOWY010000000
VERSION     DAOOWY010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16441
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3557_length_16441_cov_8.430245"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PK105_04155"
     CDS             complement(<1..294)
                     /locus_tag="PK105_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOU60933.1"
                     /translation="MAEQKKKGALDWYFKSNLLTRILIGLVLGAIVGIILGFFPSSVK
                     PYVDNTKFFGDLFIRLLKMIIMPVILFSLIAGAASIAPSKLGRVGVKIMVYYLL"
     gene            463..1179
                     /locus_tag="PK105_04160"
     CDS             463..1179
                     /locus_tag="PK105_04160"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOU60934.1"
                     /translation="MKKATIGILGGMGPEATASFFSMLVRLDRATSDQDHIHIIVDSD
                     PSIPDRTKYLLEGGANPLPAMLGSARRLLAAGADIAGMPCMTAHALLDQLRHNCTLHF
                     LSAFEETGLAIRELSPPIRNLGVLATMGTRQAKLFETAFPDKNIIWPDEALHLSLVME
                     AIYGKRGIKAGYLGEEPKHLLLEAVGFLKKHGAEAIVAGCTEVPLVLSQKDLDMPFID
                     PMYYLANALIATSRKGILER"
     gene            1276..2145
                     /locus_tag="PK105_04165"
     CDS             1276..2145
                     /locus_tag="PK105_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60935.1"
                     /translation="MLFRDRCDGIRVTGQHALNALMPYMMRGRNESAVYYEKEINIEN
                     ALSYLKKIKTSQTSKKDQDRFTLFGLIMTVALRTITLYPQLNRFVHRRALYQRKHICF
                     SYIVKQDFTIEAPEVNAKIYLDPVDSVSSINARVSDAILEARIHGEGEGERFANIFHK
                     IPGGKAVLMKIYGLLDSLNLAPWSLLRMDPLYTSIYFANLGSLGLDAPYHHLYEWGTA
                     SIFVAIGKAFSKELWHGGMRTRQRYIDLKITLDERIADGFVFAQAASTFQRLISRPEL
                     LDLSLEDLRRTLD"
     gene            complement(2175..2486)
                     /locus_tag="PK105_04170"
     CDS             complement(2175..2486)
                     /locus_tag="PK105_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU60936.1"
                     /translation="MPTYEYECRECGYSFESFQSINDAPLETCPLCGGAVKRLIFGGS
                     GIIFKGPGFYITDSKKSQSAVTCSSSEKDSSFSENTSSSESTKNGTKETPKIDPSKKA
                     S"
     gene            complement(2674..3903)
                     /locus_tag="PK105_04175"
     CDS             complement(2674..3903)
                     /locus_tag="PK105_04175"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOU60937.1"
                     /translation="MYGKIRDELTKDLEELHTEGTYKDERVIVTPQGATIRVSDGAEV
                     LNFCANNYLGLANDPSIREAAVKAMEEFGYGLSSVRFICGTQVQHKKLERRIADFLGM
                     EDSILFSSCFDANGAVFEPLLDEESAIITDSLNHASIIDGVRLCKAKRWIYKHADMRD
                     IEERDPDTGKPLKGLERCLKEAQGSHRIMIATDGVFSMDGDIANLNAICDLAEKYGAL
                     VMVDDSHATGFMGATGRGTWEQCRVGGRVDIITTTFGKALGGASGGVIASCKEIVEYM
                     RQKGRPYLFSNTLAPAIVGGTLRALDLITSSSELRDRLDFNTKLFRSLMTEAGFDIRE
                     GEHPICPVMLYDEKLAHRMADELLSEGIYVIGFSFPVVPKGRARIRVQMSAAHTQEQI
                     IKAVEAFKKVGKELGVI"
     gene            complement(3934..4488)
                     /locus_tag="PK105_04180"
     CDS             complement(3934..4488)
                     /locus_tag="PK105_04180"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU60938.1"
                     /translation="MRVRYNAPVTLTFTLCAAIVLILSQTIMPAILPSIFSTPTPFRS
                     NEFMDYIKLFTHVLGHANIQHFISNFTMILLLGPILESAYGSGFLLISILITALVTGL
                     SNAFLFPNVVLLGASGVVFMMIMLSSITNFSKGEVPLTFILIMIIYLGGQIWDALTKQ
                     DNISQFSHIIGGLVGSFLGFYHKK"
     gene            complement(4562..5344)
                     /locus_tag="PK105_04185"
     CDS             complement(4562..5344)
                     /locus_tag="PK105_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOU60939.1"
                     /translation="MIKSFSWNVNGLRACAEKGFIDWVEMTAADFICIQEIKLQEPQL
                     TDELRHPMAFNGKRYDSYWAFSEKKGYSGTGLYVLREPVSVTGLGISEFDSEGRTVIA
                     DYGGFLIISAYFPNSQEAGMRLDYKLRFCDAILELCNFERSKGKHVIVAGDFNIAHKP
                     IDLARPEQNEGNAGYLPEERAWMDKFISIGYIDTFRWFCKEPGRYTWWSYRSPKAREK
                     NIGWRLDYHCVDPEFSSALLAADIHAEIMGSDHCPVSLFLDL"
     gene            complement(5527..5829)
                     /locus_tag="PK105_04190"
     CDS             complement(5527..5829)
                     /locus_tag="PK105_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60940.1"
                     /translation="MDSKTEQNIGLSLGKTHYVQPRWRSMLAEDIYRHYKNEEKDSYV
                     RALAKLQEEDEEILLPPERGPIVFRDGVFQIDMSRIEEKGDIDLELKKLIDTIMKP"
     gene            5913..8537
                     /locus_tag="PK105_04195"
     CDS             5913..8537
                     /locus_tag="PK105_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOU60941.1"
                     /translation="MQILVCPIFVTIEQTCYTRYMSKKQSAVMEAATRFTPEVQDELR
                     SIVLAMEGREVFAVGMLNSKGLVENLEILGRGTEGAVPAPFQRRSYAQVLIHNHPSGI
                     LFPSDADVVVAAEASAEGFGSYIVDNEVSHVLVVAEPIKPKLIRSLDADEIAAVLDSH
                     GKLSHIMPEFEARLSQVEMAHDVTEIISDGGILVAEAGTGVGKSFAYLIPALAWAIGN
                     SEPVVISTATINLQQQIYKKDFPIVSSLFKKPAKAVVVKGRGNYLCKRRLYAAIEEDA
                     LFSDSSMKLREILEWDNGGGSGDKSDLALPDDDPIWSRVCSESDYCLSLHCPYHDKCH
                     VIHIRLEAASAQLIIANHHVLLADLEAKRIREGIINTVLPSYQALVIDEAHALEASAT
                     SLFSETFSKRSIQRLLLRLSRHKKRIQVGILASLSKLPDIPSMLIDAARLQLENTESS
                     ADAFNAVAATCFLENESSVLVKNLSGLNRTMFLSALQNLEKEIALLVTRLGEISEAIA
                     QELEDEESVIELRITLRSLEEMAALLAQFIKPESEPGSIFWLQVDKKNPKEPIVICSA
                     TPLDVAPILSERLFEKIRSCICTSATLTINGSFQWWRSRVGLLERGISETRLLEAGQS
                     KSNRDLKNRLSGQTLERNSIQNPRILQKNYPSPFPYSKNAMLAIDTEATPPDSPEFQA
                     YLNTAVAKLLKASRGRALVLFTSHKALRDTYNAVSPLLEQEGVLALRQGQKDRYSLLR
                     TFVNDISSVLFATESFWEGVDAPGETLSLVIITRLPFRVPTDPIQSSRAAAIDARGGN
                     SFAEMSIPEAVILFKQGFGRLIRHSQDRGVVAVLDVRILKKAYGSLFISSLPRCRLAM
                     GALDSICRDVAEFLDNER"
     gene            8546..9799
                     /gene="manA"
                     /locus_tag="PK105_04200"
     CDS             8546..9799
                     /gene="manA"
                     /locus_tag="PK105_04200"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOU60942.1"
                     /translation="MNEEDDTIDAQRQRFQCIGLLKNSAQQYVWGDTQGITPFVEAHP
                     VPGYPIGEVWMGSHERAPSKLIFNNSELALDRLIRQDPTYWLGDHISTKYDDLPFLFK
                     VLAAGSPLSLQVHPNLEQAEIGFSREEAAGIGRLTPERTFKDPHHKPELAVALTSFKA
                     LAGFRPIEEICYLLGHRLCDLLDFVGKDRSELRSLIRRLFRIRGEEYSAFETELNYRA
                     KQLMYSKQEAVRDAAHWVLELQSRYPGDPGQFAPLFLDLLQLEPGQGLFVPAGVIHSY
                     LKGSILEIMACSDNVIRAGLTSKHMDVDLLCDILDPETKPMLISPEYQRFEWGTLAIY
                     KTPAKEFRLEYFEANSDASFFPIAIASKGPKILLCIKGSFRLCTREQIELSARKSCFI
                     AGSCEKIELRGQGKLWLATVGEIDT"
     gene            9796..10383
                     /locus_tag="PK105_04205"
     CDS             9796..10383
                     /locus_tag="PK105_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HOU60943.1"
                     /translation="MNSVSHPELTGSNLGQPSFSEEHGSDPTITIWVDADSLPKDIRP
                     MLIRRAMAKRKYDGVTILVRFVASQNLLEIPGDYLILVQPGEGAADLYIETHAEQNDI
                     AITRDIPLAERLLAHSVNPMNDRGDIFTAENISERRSLRDAMAYMRDSGIAPPSPKGN
                     LRKPSDIKSFADGLEKLIVQSVRSKRRCLENPPRS"
     gene            complement(10380..11468)
                     /locus_tag="PK105_04210"
     CDS             complement(10380..11468)
                     /locus_tag="PK105_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOU60944.1"
                     /translation="MKPESQSSEARALKVKKNGKARKPVWKILRIALLAILIVHLVFI
                     GLTSALIFAYKFANPPTTVLIIYRSAVSHWEVQKPIPLKTEQIPQYIRRMLISVEDGG
                     FYEHHGIDIEAIKRARELNEKVGKPMYGGSTITMQVARTLFLLPTKSYIRKYFEAITA
                     LELELILSKDRILELYFGYAEWGKGIFGIERASRVYFGKGVASINRDQAARLIALLSS
                     PIKYTPDTLYRSAILRQRYAYLVARYVAAVPVAVVSAETPPPSGIEPSADISELSDEE
                     EDSKTEQEVSPNDGELLEDTSSDLSPIDSSPTDSSPIDSDDGASGDTSSDLSPMDSVE
                     PSPEIDNLQETKDKEKADEPSISGTPEF"
     gene            complement(11486..12022)
                     /locus_tag="PK105_04215"
     CDS             complement(11486..12022)
                     /locus_tag="PK105_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU60945.1"
                     /translation="MTEKTFMTGFGGQGIISMGQLWVYCAMKEGLKVTFFPFYGAEKR
                     GGIARASVIVSDSEITSPIVSVADSVVIMNQDSVEIGEKVCKEGGIICVNSSLVKKDP
                     ARADAKIYHIPCNEIALKLGNIRVANMIMMGALSKITGAVKLQDLENILPNYFSSSKR
                     SIIEINIAAVEEGKKVVA"
     assembly_gap    12083..12092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12093..12776)
                     /locus_tag="PK105_04220"
     CDS             complement(<12093..12776)
                     /locus_tag="PK105_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU60946.1"
                     /translation="MEIAYGHPKSLKSIQTKYCPGCGHGVIHRIIAEVVDEMGLQSKT
                     IITNPVGCSIYADMYFDFDSVQPAHGRTPAASTGIKRMLPDHLVICYQGDGDLAAIGT
                     AEIIHAANRAEKFTTIFVNNAIYGMTGGQMAPTTLVGQHATTAPEGRDPINAQMGYPI
                     RVCELLASLEGTKYLARGAVNNMVNIRRTKKYIRKAFEAQMRGEGFSLIEILSQCPTN
                     WQMNPVESVE"
     gene            complement(12792..13859)
                     /gene="vorB"
                     /locus_tag="PK105_04225"
     CDS             complement(12792..13859)
                     /gene="vorB"
                     /locus_tag="PK105_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOU60947.1"
                     /translation="MAEEIRLMKGNEAIGEAAVRAGCRAYFGYPITPQNELTAYMAKH
                     MLARGRTFIQAESEVAAINMVYGAASTGARAMTSSSSPGVSLKQEGISYLCGADLPAV
                     VIDVARAGPGLGGISPAQGDYFQATRGGGHGDYYTIVLAPKGVQEAADLTYEAFGLAE
                     KWRMPVLILADGLIGQMMEGVVLPEPMDPANLPRKSWAVGHSVEMARPFNHISSINLI
                     PDELEAINRKRFERYAQIKAELIRYEELEVEDADIVFVAYGTSARVSHGALQMARAKG
                     MKIGLFRPITLWPFPYKRLSELAEQGKRFLCVEMSMGQMIEDVRLAVGGKAPVYFYGR
                     CGGNIPSQEEVFAEALRLSSK"
     gene            complement(13871..14086)
                     /locus_tag="PK105_04230"
     CDS             complement(13871..14086)
                     /locus_tag="PK105_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU60948.1"
                     /translation="MAIKGSIEIDRERCKGCLLCIRACPTKVLGVDNEPNSWGYFPSS
                     VVAQEKCIACGNCYDVCPDVAITVYKL"
     gene            complement(14103..15029)
                     /locus_tag="PK105_04235"
     CDS             complement(14103..15029)
                     /locus_tag="PK105_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOU60949.1"
                     /translation="MNHISDIIEEARKIGRRKFSVACAQEATVLEAVTEAYREGIGEP
                     ILVGNPKAIEEAAKEANEGKGVDISPFRIIEENNIEKAAAIAVGLIRSGEAEFLMKGI
                     IDSSLYLRAALNKETGLNAGKIACHVAVMEVPTYHKLFAITDIALNIAPDLPTFIDMI
                     NSSVKVMKIIGVNNPKVAILAAVEKVNPDKMPCTATAAILTQMNRRDQIKNCIVDGPL
                     ALDNAISCESVRIKKIKSEVAGDADILVTPEIESGNILYKCLLHLAGAKGAAVIMGAS
                     VPLVLTSRADDAETKLASIALASLLGAGGTQA"
     gene            complement(15026..16099)
                     /gene="buk"
                     /locus_tag="PK105_04240"
     CDS             complement(15026..16099)
                     /gene="buk"
                     /locus_tag="PK105_04240"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOU60950.1"
                     /translation="MQSVVLVINPGSTSTKFAVFHGDRCIFDQTLNHSAEELSHFATI
                     ASQYEFRESAIQEALKARNFDMSSLNAVVGRGGLLHPIPGGIYRINEGMKADLLAAKY
                     GEHASNLGALIADAFAKQFGIPAFIADPVVVDELEDVARVSGHKLFKRISIFHALNQK
                     AVARRFAKERGVCYEDLTLIVAHLGGGISIGLHRQGKVIDVNQALSGEGPFSPERSGT
                     LPAGDLARLCFSGQYTIQEVLKMITGKGGMVSFLGTNDMREVERMRDEGNKDADFYFQ
                     AFVYQVGKYIGSLAPVVEGKVDAIILTGGIAFWKELTERISQMVSFIAPVVVYPGEGE
                     LEALALAGQIALEGEADIKEYVP"
BASE COUNT         4348 a         4011 c         3641 g         4431 t
ORIGIN      
        1 gagaagataa tagaccatga ttttcacgcc tactcttccg agcttagatg gtgcgatact
       61 cgcggctcct gcgatgagcg aaaacagaat tacaggcatg ataatcattt tgagtaacct
      121 gataaacagg tcaccaaaaa atttcgtatt gtcgacatag ggttttaccg aggaaggaaa
      181 gaatcccaaa ataataccga caatagctcc caacaccagg ccgatcagta ttcgcgtcaa
      241 tagattggac ttaaagtacc aatccaaggc gcccttcttc ttttgttcag ccatgcagac
      301 ctccttgctt tattgatgtt cataaagcca tatgattatg gatccgaatt cgccagaccg
      361 caagagggaa aaatcttcaa gctataattt cattgcagtg gtatttggta taggcatctc
      421 ctaatttaca tggatccctg tgttatgtaa catgaatacg ctatgaaaaa ggcaacaatc
      481 ggtattctgg gtggaatggg gccagaggca acggctagct tcttttcgat gctagttagg
      541 ctcgatagag caacttccga tcaagatcat attcatatca tcgtggattc ggatccttcg
      601 attcccgata ggacaaagta tcttctcgag ggaggcgcaa atcccctccc tgcaatgctt
      661 ggctcagcca gaaggttgct cgccgcaggc gctgatattg ctggaatgcc ctgcatgaca
      721 gcccatgcac ttctggatca actccgccac aactgcaccc tgcattttct ctcggccttt
      781 gaagagaccg gcctcgctat tagggaattg tctcctccca ttcgaaatct tggtgttctt
      841 gccacgatgg gaacgcgcca ggcaaagctt ttcgaaacgg cttttccgga taagaatata
      901 atatggcctg atgaagccct acacctttcc ttggttatgg aggcaatcta tggtaagcgc
      961 ggtatcaagg caggctatct aggcgaagag ccaaagcatc tcttgctgga agcggttggc
     1021 tttcttaaaa agcacggcgc agaggctatt gtagcaggct gtacggaagt gcctcttgtt
     1081 ctgtctcaaa aagacctcga tatgccattc atagatccca tgtattatct agcgaacgcg
     1141 ctgatagcaa catcccgcaa gggtatcctt gaaagataat tggagctggt gaaaagatat
     1201 tcgatcgaga tagatgcagt ccggacactt gctccagttt actcatgtgt gcttttctca
     1261 tatatttgaa tgacaatgct gttccgagat cgctgtgatg gaatacgcgt aactggccaa
     1321 catgctctca atgcgctaat gccatatatg atgcgtggcc gcaatgaatc tgctgtctat
     1381 tatgagaaag agataaatat cgaaaatgcc ctttcatacc ttaagaaaat taagacaagc
     1441 cagacatcaa aaaaagacca ggatcgtttt accctttttg gcctcattat gaccgtagca
     1501 ttaaggacaa tcactcttta cccgcagctc aatcgttttg tccatagacg agcgctctat
     1561 cagcgaaaac atatttgctt ttcctatatt gtcaagcagg atttcaccat cgaggctccc
     1621 gaagtaaatg ctaagattta tctagatcca gtggattcag tctcgagcat caatgcaaga
     1681 gtgagcgatg caattcttga ggcacgtatt catggagaag gcgaaggcga acgtttcgcg
     1741 aatatcttcc ataaaattcc tggtggtaaa gcagtactga tgaaaatcta tggactgcta
     1801 gatagcctaa accttgcacc ctggagtctt ttacggatgg atcctctata tacaagcatc
     1861 tattttgcaa acctgggctc acttggtctc gatgctccct accatcacct ctatgagtgg
     1921 ggcaccgcca gcatatttgt cgctataggc aaggcttttt caaaagagct atggcatgga
     1981 ggaatgcgga ccagacagcg ctatatagac ttaaaaataa cgctcgatga acgtattgca
     2041 gatggctttg tatttgccca ggctgcatct acattccagc gcttaatttc taggcctgaa
     2101 ttgttggact tatctcttga agatctcaga agaacattag actaggctca gccgagccat
     2161 ttgttgcaac ttatctagct cgctttcttg gatggatcaa tcttgggggt ttctttggtt
     2221 ccatttttag tgctttcgga agagctggtg ttctcggaaa atgacgagtc cttctcagag
     2281 ctcgagcatg ttaccgctga ttggctcttc ttagagtcgg tgatatagaa tcccgggcct
     2341 ttgaatatta tgccgctgcc cccaaagatg agccttttta ccgctcctcc gcatagagga
     2401 caggtttcaa ggggagcatc gtttatcgat tgaaatgatt cgaatgaata gccgcattcc
     2461 ctgcactcat attcatatgt aggcatatcg ctcacctcta agagaaaaaa tcgatttaga
     2521 tttccataaa aaatatcaat aatccgaata aaggtaaaga gtatatgatc cgctggccat
     2581 agaattcaag agatctttaa catagaggag gtatataact cgcccctcac agaacccaag
     2641 ggctcttcaa catagagaag atcattcata agatcagatc actccgagct ccttacccac
     2701 ttttttaaaa gcttcgaccg ctttaatgat ctgctcttga gtatgagctg ccgacatctg
     2761 aacgcggatt ctagctctgc cttttggtac aacaggaaaa gaaaagccta ttacataaat
     2821 gccttcggaa agaagttcat cggccattct atgagcaagt ttttcgtcgt acagcattac
     2881 ggggcagatt gggtgttcgc cttctcttat atcgaaacct gcttctgtca taaggctacg
     2941 aaatagcttg gtattaaaat cgaggcgatc tcgcagctct gaagaactag ttataagatc
     3001 gagtgcccgc agagtaccac ctacaatggc cggggccagg gtgttcgaga aaagatatgg
     3061 acgccctttc tgtcgcatgt attcgacaat ttccttacag gaagcaataa ctccgcccga
     3121 ggctcctcca agtgctttcc cgaaggtcgt agtaatgata tctaccctac caccgactcg
     3181 acattgttcc caggtgccac gtccagttgc ccccatgaaa ccagtcgcat gcgaatcatc
     3241 caccatcacg agcgcaccat atttctctgc caaatcgcaa attgcattca gattggcaat
     3301 gtcgccatcc atggagaaca cgccatcggt agcgatcatt atcctatggc tgccttgggc
     3361 ttcctttaga cagcgttcga gcccttttag aggtttccct gtatcaggat ctctttcttc
     3421 gatatctctc atatcggcat gcttgtatat ccagcgtttt gccttacata gacgcacacc
     3481 atcgattatg gaagcatgat ttaggctatc ggttatgatt gccgattcct catcaagaag
     3541 aggttcaaaa acggctccat tcgcatcgaa gcaagatgaa aagagaattg aatcttccat
     3601 gccaagaaaa tctgctattc tccgctcgag ttttttgtgt tgtacctgcg ttccacagat
     3661 aaaacgaaca gaagagagtc catagccaaa ttcttccata gccttcacag cagcctcgcg
     3721 gatgctcggg tcattcgcaa gaccaaggta gttattcgcg caaaaattca gcacttccgc
     3781 accatctgaa acacgaatag ttgcgccttg cggcgtaacg atgacacgtt catctttgta
     3841 ggtaccttct gtatgaagct cttcgagatc cttcgtaagt tcatctctta ttttaccgta
     3901 cataatgaca cctcatattc gattttcttg tattcacttc ttatgataga aaccaaggaa
     3961 gctgccgaca aggcctccta ttatatggga aaactgagaa atattgtcct gttttgtaag
     4021 agcatcccaa atctgtccgc ccaaatatat gatcatgatg agaatgaatg taagtggcac
     4081 ctcacctttt gagaaattgg tgatagacga aagcatgatc atcatgaaca ccactcctga
     4141 agcgccaaga agcaccacat tcgggaaaag aaaagcattt gaaagccctg ttacaagcgc
     4201 tgtgatgagt atgcttatta gcaaaaaacc cgaaccatat gcgctctcca aaataggtcc
     4261 cagaagcaag atcatcgtaa agttgctaat aaagtgttga atattcgcat gcccaagtac
     4321 atgcgtgaaa agcttgatat aatccatgaa ttcattgctt ctaaatggtg tgggggtaga
     4381 aaaaatcgaa gggagaatcg caggcataat agtctggctc aatatgagca caatagctgc
     4441 acataaagta aaggtaagcg taaccggcgc attgtatcga acacgcatgc caaaccctcc
     4501 agaagaacaa aaaacaggaa gccgacttcg agccggtcat tttttattta attcgatgct
     4561 atcacagatc gagaaaaaga gacactgggc aatggtcgga tcccatgatt tcggcatgta
     4621 tatcagccgc aagcaaagct gacgagaatt ccggatcgac acagtggtaa tccaggcgcc
     4681 acccgatatt cttctctcta gcctttgggg agcgataact ccaccaggta tatctccccg
     4741 gctccttgca gaaccatcgg aatgtatcga tgtagcctat agagatgaac ttgtccatcc
     4801 aggcacgctc ttcgggcaaa tatccagcat tgccttcgtt ttgctcaggt cgggccagat
     4861 cgataggttt atgggctata ttaaaatcac ccgcaactat aacatgcttg ccttttgaac
     4921 gctcgaaatt gcatagctca aggattgcgt cacagaaacg gagcttatag tcgagccgca
     4981 tgcctgcttc ctgactgttg gggaaataag cacttataat gagaaatcca ccgtaatcgg
     5041 caatgacagt tctgccctca gaatcgaatt cggagatacc caggccggtc acggaaaccg
     5101 gctcgcgaag cacatagaga cctgttccag aataaccctt cttctccgag aaagcccagt
     5161 aactatcata tcgcttccca ttgaatgcca tgggatggcg aagctcatca gtaagttgcg
     5221 gctcctgtaa tttaatctcc tgtatacaga taaaatccgc agcagtcatc tctacccagt
     5281 ctataaagcc cttttcagcg caggcgcgga gaccattcac attccatgaa aagctcttga
     5341 tcataaacct tgtccctcag ttgtcttttg tcttatatgc tgcattggcc gcatggagag
     5401 ctttttgcgc atttcgcctt gtatttcgcg cgcatttcaa tctgtatttc gcccgcattt
     5461 caatctgcat ttcgctcgca tttcgccctg cattgacatc aaagacacat tcctaacatg
     5521 ccctcttcaa ggtttcataa tagtatcgat taattttttc agctcgagat ctatatcgcc
     5581 cttctcttct atgcggctca tgtcgatctg aaatactcca tcgcggaaga ctatgggtcc
     5641 tcgctcgggc ggcaagagaa tctcttcatc ctcttcttga agtttcgcca aagcgcgaac
     5701 atagctgtct ttttcctcat tcttataatg gcggtatata tcctctgcaa gcatagatcg
     5761 ccagcgaggc tgcacataat gggtcttgcc aaggcttaag ccgatgttct gttcagtttt
     5821 ggaatccatc tgagtctata ggatatactc ggagtcttag tcttcgcaac taccttgcac
     5881 agcatcgaac cttccatccg gatttacgat aagtgcagat tctggtttgt ccaatattcg
     5941 taacaatcga acagacatgc tatactcgct atatgagcaa aaagcagtct gcggtgatgg
     6001 aagcggccac gcgttttact ccagaagttc aggatgagct taggtctata gttctcgcca
     6061 tggaaggtag agaggtattc gcggtaggca tgctcaattc taaaggtctt gtagagaatc
     6121 tagagatctt aggccgtggc actgagggag cagtacccgc gccctttcaa agacgttcat
     6181 atgcacaggt gcttatccac aaccatccct ctggaatact attcccttcg gacgcagacg
     6241 tcgtggtagc ggccgaggca agcgccgaag gctttggcag ctatattgtt gacaacgaag
     6301 tctcacatgt gctcgttgtc gctgaaccga taaaacccaa actcatccga tccctggatg
     6361 cagacgaaat cgcagccgtg ctcgattctc atggaaaact gtcacatatc atgcccgaat
     6421 tcgaagcgcg gctctcacag gtagagatgg ctcacgatgt aacggagatt atctcagatg
     6481 gaggtattct tgttgccgaa gcgggtactg gcgtaggaaa atcctttgca tatcttatac
     6541 cggccttagc ctgggcaatc ggaaacagcg aacctgttgt gatctctacg gctactatca
     6601 atcttcaaca acaaatctat aaaaaggatt ttccgatagt gagctccttg ttcaagaaac
     6661 ccgcaaaggc agttgttgtc aaggggcgag ggaattatct atgcaagcgc cgactctatg
     6721 cggcaataga ggaagatgct ctcttttccg attcttcgat gaaactcaga gagattctcg
     6781 aatgggacaa cggtggagga agcggagata agtcagacct tgctctgccg gatgatgatc
     6841 ctatatggag tcgcgtatgc tccgaaagcg attattgtct atccctgcat tgtccttatc
     6901 acgacaaatg ccatgtaatc catataaggc tggaagcggc aagcgcacag ctcatcatag
     6961 ccaatcatca tgttttacta gcggacttag aagcaaagcg aatacgagaa gggatcatca
     7021 atacggtttt gccttcgtat caggcacttg tgatagatga agctcacgca ctcgaagcaa
     7081 gtgcaacgag tctcttttcc gagacttttt caaaaaggtc tatccaaaga ttgcttctta
     7141 gactttcaag acataagaag cgaatccagg tgggaatatt ggcttcgcta tcgaaattgc
     7201 ccgatattcc atcgatgctg atcgatgctg ctcgtcttca attagagaat accgaatcat
     7261 cagccgatgc tttcaatgct gtcgctgcaa cttgcttttt ggaaaatgaa tcaagcgttc
     7321 ttgtaaagaa tttgtctggc cttaatcgca ccatgtttct gtcagctctt cagaacctcg
     7381 aaaaagagat cgccttgcta gtaacgcgac ttggcgaaat ctctgaagca atcgcccagg
     7441 aactggaaga cgaagaaagt gttatagaac tcaggatcac acttcgatct ctggaagaga
     7501 tggcagctct attggctcaa ttcataaaac cagaatcaga acccggttcc atattctggc
     7561 ttcaagtcga taaaaagaat cctaaggagc ctattgtgat atgcagcgct actcctctag
     7621 atgtggctcc catactctcg gaacgacttt ttgaaaagat tcgtagctgc atttgcacaa
     7681 gcgcaactct cacgatcaac ggctcctttc aatggtggcg gagcagagtc ggattattgg
     7741 aaagaggaat atcagagact agattgttag aagcaggaca atccaaatcc aacagggatt
     7801 tgaagaacag actatccggc cagaccttgg aaagaaactc catacaaaac ccaagaattc
     7861 tgcaaaagaa ttatccttcg ccatttccct attccaaaaa tgcgatgctt gctatagaca
     7921 ccgaggctac tccgccggat agccctgaat tccaagccta cttgaacacg gccgtcgcta
     7981 aactgctcaa ggcttcgcgc gggcgtgcac tcgtgctttt taccagtcac aaggcacttc
     8041 gcgacacgta caacgcggtc tctccacttc tggaacaaga aggcgtattg gcccttcgac
     8101 agggacagaa agataggtat tctttgcttc gaacctttgt caacgacatt tccagcgtcc
     8161 tttttgcgac agaatccttc tgggaaggtg tagatgcacc aggcgagact ctttcgttgg
     8221 tcattatcac cagacttcca tttcgcgtac caaccgatcc aattcaatcg tcgagagcgg
     8281 ctgcaatcga tgctcgaggc ggaaattcct ttgcggagat gagtatccct gaggcggtaa
     8341 ttcttttcaa gcaaggcttt ggacggctta tccgccattc gcaggatcgt ggggtagttg
     8401 ctgttctcga tgttcgtatc ttaaaaaagg cctatgggag tctttttatc tcgagcctgc
     8461 ctagatgcag actcgcgatg ggggctctgg actctatttg tcgcgatgtg gcagaattcc
     8521 ttgataatga aaggtaatcg attcgatgaa cgaagaagac gatacaattg atgctcaaag
     8581 gcagcgtttc cagtgcatag gactactgaa aaattctgcc caacaatatg tatggggcga
     8641 tacacaaggg attacgccct ttgtggaagc acatccggta ccgggttatc ctattgggga
     8701 ggtttggatg ggaagccacg aaagagcacc ctctaaacta attttcaata attcggaact
     8761 ggctcttgat aggctaataa gacaagatcc aacttactgg cttggagatc atatttctac
     8821 gaaatatgat gatttacctt ttctattcaa ggtactggct gcgggttctc ctctatccct
     8881 tcaggtccat ccaaacctag aacaggcgga gataggattt tcaagagaag aagctgccgg
     8941 cattggccga ttgactccgg agcgaacatt caaggatccg caccataagc cagaactagc
     9001 cgtggcactt acatcattta aagctctggc tggtttccgc cccatagaag aaatatgcta
     9061 tcttttgggt cataggctat gcgatttact cgattttgta ggaaaagata gaagtgagct
     9121 ccgtagctta ataaggcgtt tatttcgcat tagaggtgag gaatactcag cctttgagac
     9181 cgaattgaat tatcgagcca aacaactcat gtattcgaaa caagaggccg tccgagatgc
     9241 tgcacattgg gtgttagagc ttcaatcgcg ctatccaggt gatccaggac aatttgctcc
     9301 cctttttctc gatttgctgc agctggaacc tggccagggt ctattcgtac cagctggtgt
     9361 gattcactct tacctgaagg gcagtatcct tgagattatg gcttgttcag ataatgtcat
     9421 aagggcaggt ctcaccagta agcatatgga tgtcgatctt ctatgcgata tcctcgaccc
     9481 ggagacgaaa cccatgttaa tctctcccga ataccaacga ttcgaatggg gaacccttgc
     9541 catctataag actccagcga aggaattccg ccttgagtat ttcgaggcga attccgacgc
     9601 atcgtttttt ccgatagcaa tcgcttctaa aggaccaaag attcttctat gcatcaaagg
     9661 aagctttaga ctttgtacca gagaacaaat cgaattgtca gctcgcaaat catgctttat
     9721 tgcaggatcc tgcgaaaaga tcgaactgag aggacaaggc aagctctggc ttgctaccgt
     9781 tggcgagata gacacatgaa ttcggtttcg cacccagagc taacaggctc taatctgggt
     9841 cagccctcct tctcagagga gcatggatct gatcccacaa ttactatctg ggtggacgcc
     9901 gattcacttc caaaagatat caggccaatg ttgataagaa gggctatggc aaaaagaaaa
     9961 tacgatggtg ttacgatcct tgtgcgcttc gtagcaagcc aaaaccttct ggaaattcca
    10021 ggagattatc tcatccttgt ccagcctggc gagggagctg ctgatctcta cattgaaact
    10081 catgcagaac agaacgacat cgccataacc cgagatatcc ctctcgcaga acgccttcta
    10141 gcgcattcgg ttaatccaat gaatgatcgc ggcgatatct ttactgcaga gaatatcagt
    10201 gaacgacgtt ctcttagaga tgctatggct tatatgcgag attcgggtat cgctccaccc
    10261 agccctaaag gcaacttgcg aaagccatcc gatataaaga gctttgcgga tggtctcgaa
    10321 aaactcatag tccaatctgt gagatcaaag agacgatgct tagagaaccc acccaggtct
    10381 taaaattcag gcgtccctga aatcgatggt tcatctgctt tttctttgtc cttagtctct
    10441 tgcagattat ctatctccgg agagggctca acagaatcca ttggggataa atccgaggat
    10501 gtatcgccag atgctccatc atcagaatct attggagatg aatctgttgg agatgaatct
    10561 attggagata gatccgaaga tgtgtcctct aatagttcac catcatttgg agagacctct
    10621 tgttctgttt tagaatcctc ctcttcatcg gatagctccg atatatcagc cgaaggctct
    10681 attccggatg gtggaggtgt ctcggcagag acaacagcta caggcactgc tgctacatat
    10741 ctcgcaacaa gataggcata acgttgccgc aagattgcag agcgatagag agtgtcgggt
    10801 gtatatttta ttggagaaga cagaagggct ataaggcgag ccgcttgatc ccgattgata
    10861 cttgctacac ctttgccaaa atagacccga gaagcgcgct cgattccaaa gatcccttta
    10921 ccccattccg cataaccaaa atagagttcg aggatgcgat ctttcgagag aatcaattcc
    10981 agctctagcg cagtgatggc ttcgaaatac ttgcgtatat agctcttagt aggcaaaagg
    11041 aaaagggtac gcgcaacctg catggtgatc gtgcttccac catacattgg tttccccact
    11101 ttttcgttga gctctcgtgc tcttttgata gcctcgatat cgatgccatg gtgctcatag
    11161 aatcccccat cctctaccga tattagcatc cttcttatat attgaggaat ttgctcggtc
    11221 tttagaggga tgggtttttg cacttcccaa tgacttactg ccgatcgata aataatgagc
    11281 actgttgtgg gaggattggc aaacttatat gcaaatatta atgcgctcgt caaacctata
    11341 aatactaaat gcacaatgag tattgccaat agggctatac ggagtatttt ccaaaccggc
    11401 tttcgagctt tgccgttttt ctttaccttt agtgctcttg cttctgacga ctgcgattca
    11461 ggcttcataa ggtaagagca ttatatcacg ctactacctt ctttccttcc tcaactgcgg
    11521 caatattgat ctcaatgatt gagcgcttac tcgaagaaaa atagtttggc aatatgtttt
    11581 ctaggtcttg tagcttaacg gctcccgtaa tcttgctcaa ggctcccatc attatcatat
    11641 ttgctacacg gatattgccg agcttcagtg ctatctcgtt gcatggaata tgatagattt
    11701 ttgcatcagc acgggcagga tctttcttga cgaggctgga attgacacag atgattccgc
    11761 cttctttgca aaccttctct ccaatttcca cggagtcctg attcattatc acgactgaat
    11821 cggcaacgct gacaataggg cttgttattt cgctgtctga gactataacc gatgcgcgtg
    11881 ctattccgcc tcgtttctcg gctccataga aggggaaaaa cgtcactttg aggccttctt
    11941 tcattgcgca ataaacccaa agctgcccca ttgaaataat accctggcca ccgaagcctg
    12001 tcataaaagt cttttcggtc atggaacact cctttcaatg cgcctgcgca ggggctgcag
    12061 ccgtgctgag agtatctttg atnnnnnnnn nnttcgacag actccacggg attcatctgc
    12121 cagtttgttg gacactgtga gagaatctcg atcagggaaa aaccttcgcc gcgcatttga
    12181 gcttcgaagg ccttgcgtat gtatttcttt gtccttctaa tattgaccat attgtttacg
    12241 gcgccgcgcg caagatattt tgtcccttcg agcgaagcca agagttcgca tactcgaata
    12301 gggtagccca tttgagcatt gataggatcg cggccttccg gcgccgttgt agcatgctgt
    12361 ccgacaagag ttgtaggagc catctgccct cctgtcatgc catagattgc attgtttacg
    12421 aagattgtgg tgaatttctc agcccggtta gcggcatgga taatctcggc tgtcccaatg
    12481 gccgcaagat caccatcgcc ttgatagcag attacgagat ggtcaggcaa catccgcttt
    12541 attcccgttg aagcagctgg cgtccggcca tgtgctggct gaactgagtc gaaatcgaaa
    12601 tacatatcgg cataaataga acagccaacc ggattcgtga taatcgtctt actttgcaac
    12661 cccatctcat ctacgacctc ggctatgatt ctatggatta ctccatgacc gcagccaggg
    12721 cagtattttg tctgtatcga tttaaggctc ttaggatgcc cataagcaat ttccataata
    12781 aatccctccc ctcatttgct cgaaagtcgt agcgcttcgg cgaaaacttc ttcctggctg
    12841 ggtatattcc cgccacagcg cccatagaag tacacaggag cttttccacc gaccgcgagc
    12901 cgtacgtcct cgatcatctg ccccatgctc atctcgacac aaagaaagcg cttgccttgc
    12961 tctgctagct cagaaagccg cttataagga aatggccaga gtgtaatagg tcggaaaagg
    13021 ccaatcttca tccccttggc tctagccatc tgtagagcac cgtgtgaaac gcgcgctgaa
    13081 gttccatatg ctacaaagac tatatcagca tcctcgactt ccagctcctc ataacgaatt
    13141 agttccgctt ttatctgtgc atagcgttca aatcgcttcc tgttgatggc ttctagctcg
    13201 tcgggtatga gatttatgct agatatatgg ttaaaaggcc ttgccatctc cacagagtgc
    13261 ccgacagccc aagatttacg cggcaaattg gctggatcca tgggctccgg gagtaccacc
    13321 ccctccatca tctgaccaat tagcccatcc gcgagaataa gcacaggcat gcgccatttc
    13381 tcggcgagtc cgaaagcctc ataggtcaaa tccgcagcct cctggacccc cttgggtgcg
    13441 agaacaatcg tataataatc cccatgccca ccacctcggg tcgcctggaa ataatctccc
    13501 tgagcaggcg atataccgcc caggccaggg cctgcacgtg caacatctat gactaccgct
    13561 ggaaggtcgg cgccgcagag atatgaaata ccttcttgtt taagtgaaac acccgggctc
    13621 gaggagctgg tcatcgcacg agcacctgtg gaagcagcac catataccat attgattgcc
    13681 gcaacttcag actctgcctg gatgaaggtg cggcctctag ctagcatgtg cttagccatg
    13741 taggcggtga gctcgttctg cggggttata ggataaccaa aataagcgcg gcaccccgct
    13801 cgcaccgccg cttcgcctat cgcttcattg cctttcataa ggcgaatttc ttcagccatt
    13861 tccttctccc tcagagtttg tatacggtaa ttgccacatc tgggcagacg tcatagcagt
    13921 tgccacatgc tatgcatttt tcttgagcga ccacactgga agggaaatac ccccaggagt
    13981 tcggctcgtt atcgacacca aggacttttg tcggacaagc gcgaatgcat aagagacagc
    14041 ccttgcatcg ctccctgtcg atctcgatag atccttttat cgccattaga tactccttat
    14101 attcaagcct gagtaccccc agctcctaga agagaagcca gggcaatcga ggccagtttg
    14161 gtctctgcgt catccgcacg gctcgtgaga actagaggca ccgaagcacc cattatcacg
    14221 gctgcgcctt tagctcctgc gaggtgcaat aggcacttat agagtatatt cccagattcg
    14281 atttctggcg tgacaagaat atctgcgtcg cctgcaactt ctgattttat tttcttaatg
    14341 cgaacacttt cgcagcttat cgcattgtct aatgccagag gaccatcgac gatgcagttt
    14401 ttgatctggt cgcgacggtt catctgcgtt aggattgcag ccgttgcagt gcaaggcatc
    14461 ttgtcagggt tgactttctc gacagccgct agtattgcaa ccttaggatt atttactccg
    14521 ataattttca ttaccttgac tgatgaattg atcatgtcga taaaagttgg aagatctggt
    14581 gcaatattga gagctatatc ggtaatagca aatagtttgt gataggtcgg tacttccata
    14641 acagccacat ggcatgcaat cttgccagcg ttgagacctg tctctttatt gagagcagcc
    14701 ctgagataga ggctagaatc gatgataccc ttcattagga attctgcttc tccggaacga
    14761 ataagaccga ctgctatggc cgctgctttc tctatgttgt tttcttcaat gattctaaag
    14821 ggcgaaatat ccaccccttt cccttcattg gcttctttgg ctgcttcctc gatagctttc
    14881 gggttaccta caagaatagg ctcacctatt ccttcgcggt atgcttccgt tacagcttcg
    14941 aggacggttg cttcttgagc acaagcaacc gagaatttac gcctgccgat cttccttgcc
    15001 tcttcgatta tatctgatat atggttcatg gcacatactc ctttatatca gcctctcctt
    15061 cgagcgcgat ttggcctgct agtgccaaag cttcaagttc accttctccc ggatacacca
    15121 ctacaggcgc aatgaacgaa accatctgac taattctctc agtgagttct ttccagaatg
    15181 cgataccccc ggtcaatatg attgcatcga cctttccttc gactactggt gcgagagaac
    15241 cgatatactt acctacttga tatacaaaag cttggaaata gaagtccgca tccttgtttc
    15301 cctcatcgcg catccgctcg acctcacgca tatcgttggt gccaaggaaa ctcaccatgc
    15361 cccctttccc ggttatcatt tttagtactt cttggatggt gtattgccca gaaaaacaga
    15421 gtcttgccag gtctcctgca ggcaaggttc cggagcgttc tggcgaaaat ggcccttctc
    15481 cgctcagagc ttgattgaca tcgataactt tgccttgtct gtgcaagcca atagaaatac
    15541 ctcccccaag atgagccaca attagcgtaa gatcttcgta gcaaacacca cgctcttttg
    15601 caaatcgacg cgccactgcc ttttggttta gcgcatgaaa aatgctaatt cgcttaaaga
    15661 gcttgtgccc cgagactcgc gcaacatcct cgagctcatc cacaaccaca ggatccgcaa
    15721 taaaggcggg aattccgaac tgttttgcaa aagcatctgc tatcagagca cctaggttcg
    15781 atgcatgctc gccatatttt gcggcgagca aatcagcctt cataccctcg ttgattctat
    15841 atattccacc aggaattgga tgaagaagtc ctcctcgtcc aaccaccgca tttagcgatg
    15901 acatgtcgaa atttctagcc ttgagtgctt cttgaatcgc actctctcta aattcatact
    15961 gtgaagctat agtagcaaaa tgtgagagct cttctgccga atggttcagc gtctgatcga
    16021 aaatacatct gtcgccatga aaaacggcaa atttcgtgct tgtagaccca ggatttatga
    16081 cgagaacgac agattgcatg caatttctcc tgaattggtg gcagttgact ttaagtatat
    16141 catgtgtcta aggaaacgca agaggaaatc ttgcagaaaa tgaaagaccg tttcaaatat
    16201 caaaacgatc tttatacgct cagagatgag ccagcctagt tcagaaaaga atgaacatgc
    16261 gaaaaataaa aaggccgccc tttcgattaa ggatggcctc ttatcaagat aatatgtgcg
    16321 gcgcgttcat ttgcgggtct tatggcgatt cagtcttctt ttcttttttc tcttatgcgt
    16381 cgctattttt tggagctttc gctttctacc acagggcacc ttttctctcc ttggaaacga
    16441 t
//
LOCUS       DAOOWY010000035        15840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3725_length_15840_cov_6.686411, whole genome shotgun sequence.
ACCESSION   DAOOWY010000035 DAOOWY010000000
VERSION     DAOOWY010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15840
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3725_length_15840_cov_6.686411"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PK105_04245"
     CDS             complement(<1..319)
                     /locus_tag="PK105_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60951.1"
                     /translation="MLKPHLISTLSGASYPFQQLNEFAENGESLEVVIPNIEHARPLS
                     TSTGICDRICASSKRKAGTLGEPMARGTLIRTQNENAAASDASIWQRYADFLPFQETY
                     PELS"
     gene            complement(531..1811)
                     /locus_tag="PK105_04250"
     CDS             complement(531..1811)
                     /locus_tag="PK105_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU60952.1"
                     /translation="MLAESVLLGSFIILILLGVPISFGLGISSLLSAFAAGLQPAIIA
                     QRVAAGMQSFPLLAIPLFILAGALMANGGVAKRMVDLAYVIVGPLPGGLAIVNCIDSM
                     FFGGVSGSAVADVSSTGPMIIPMMVEKGYDKDFAVALTVASASQGIIIPPSHNMVIYA
                     MVAGGVSVGRLFLAGYIPGMLLGIALAIACYFIALKRNYPCEKRPPLKESLRIILDGI
                     LALLCAAIIIVGIAAGIFTATEASAVAVLYAAFLGFFIFKELKLKDLWPIFMDSVKTT
                     SVVFFLIGCASAFGWMMTYLQIPLAVMNFFLSLTNNRFLILLLVDVLLLLLGMVMDVA
                     PLIVIVTPVLLPVVKAAGMSEITFGIVLMLNLGIGLTTPPVGASLFTGCMIGKTTIEK
                     ASKAMLPLWPAMLAVLFLVTYVPWFTEFLPNLFY"
     gene            complement(1801..2328)
                     /locus_tag="PK105_04255"
     CDS             complement(1801..2328)
                     /locus_tag="PK105_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOU60953.1"
                     /translation="MRKDLWHIVDRLERILTGFIAILMGIVTIVVCWQVFDRYVLHKS
                     PYWAEEFSVTIMMWMGLLGAACAVWTGDHMRLELLTKHLPARFGLILEILIDGAVIYF
                     SYFLFKQGIELVNAMWTSQLSTIRLSIGITYLVIPISAAMMILFSILKIVKKASGANA
                     ISSQQETKNGGEDAR"
     gene            complement(2460..3431)
                     /locus_tag="PK105_04260"
     CDS             complement(2460..3431)
                     /locus_tag="PK105_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOU60954.1"
                     /translation="MKKKLIALAALLLITASFAIAAPKYNWKLASVLPDSHPVHQALV
                     FFADELARKSNGDIKVTLFPAGQLGQEADYIQAIKIGSLEFSKVSSGPLGQYAPTLQV
                     VSLPLIWRDLDHQHKVLQGQVGKRLMNDLDKAGFKGLVFMDAGFRSITAKKPIYTPAD
                     LKGLKIRVMQSKPLIDTINALGATAVPMGQSEVYSALQTNVIDGWENNEPTVLSFNMQ
                     EVAKYFSYTRHSSIPDILIMNKKLFDGLPKNIQTMIEETAFEAMQKHNQLWGEMIDKN
                     VAELKAKGMIFNEVKDITEFQKACAGVWKEFEPIVGKDLIDAIVNTK"
     gene            3782..4579
                     /locus_tag="PK105_04265"
     CDS             3782..4579
                     /locus_tag="PK105_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU60955.1"
                     /translation="MSFGYIHLMESLMNSLKGKKALVTGGASGIGRAISLELSNMGAD
                     LIIHYHKSRELAESLAEQIRTSGGKADVVQADLSEEKGVQILADFIFERWNNLDVLIN
                     NAGDLVGRYRLEDVRMDFYRKVVAVNLDSNVLVTRAMIPLLKRSGCASIVNLASLAGR
                     KGGHGGSIVYSMTKGAILTLTRALSVELAPYGIRVNAVAPGFILGSAFHAIHTSEESK
                     QATIRDIPLKRAGTCEDVARAVAFLASEYDGFITGATLDINGGVYMA"
     gene            complement(4648..5394)
                     /locus_tag="PK105_04270"
     CDS             complement(4648..5394)
                     /locus_tag="PK105_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU60956.1"
                     /translation="MAAARRAIRIDDIYYSNRNDLALLSFDRSATIIIGSEGGCDISL
                     LNFLKKEKNAVCIDFDPDLKNIDVVCADFGKSIGTIAKRFAELGISRIGYIGGKEISP
                     LKGKEIIDPRSAQYIEEFGKLGIYRKEIFRAYGPYTPENAYQFTKELLSGTTKPEAIF
                     VASDNMALGVYKAICEANLSIPQDIKVISCNDQPVSAYMTPPLSTIRIPRAVMGSVAL
                     SLASSRAADFRELGVKVMVPTDVVFRKSFI"
     gene            5533..6315
                     /locus_tag="PK105_04275"
     CDS             5533..6315
                     /locus_tag="PK105_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60957.1"
                     /translation="MNTSYGAECDWQNHKLLHIDRLPGRSYIKRWKDVQGALRDDESE
                     QLGFRNLNGSWKFLFLEAPELSPFGFEKPDFDDGAWEDIEVPSHWQLKGHGRPHYTDL
                     YYPFPVRPPFVPRANPTGIYRRQFFLDDLSRRQILRFHGVDSAFHVWINGKLAGFSKG
                     SRLLSEFDITSLVRVGKNVIVVRVYQWSDGSYLEDQDMWWLSGIFRDVEIYEVPLVSL
                     FDVSHEVSFDDIYKNALLKVELQIENKTEDMSPGGRCFKMFF"
     gene            6315..6521
                     /locus_tag="PK105_04280"
     CDS             6315..6521
                     /locus_tag="PK105_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU60958.1"
                     /translation="MKQHNINAVRTSHYPNHPYFLELCDYFGLYVIDETDLETHGFEW
                     IDESTRLVNDPEYNPIYKTAGEWQ"
     gene            6544..6846
                     /locus_tag="PK105_04285"
     CDS             6544..6846
                     /locus_tag="PK105_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60959.1"
                     /translation="MTDRQGFGLAIISEKPIEFSYHDYDVDALEKACYDHEIAHTAYC
                     ILNLDFAQNGIGSNSCGQDQLPPYRLKPQEFDLGLEFYALDPETSFLANAKSIGES"
     gene            6850..8535
                     /locus_tag="PK105_04290"
     CDS             6850..8535
                     /locus_tag="PK105_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOU60960.1"
                     /translation="MDLDISKTPWSAFGARFSVAIGKDGSVYLKDIGGGDEIPSELFS
                     MKLLLGKEGLDQKEELEQVIEANENECRIRAKNSPDRYVLLKFLFSDVISIECCDIRL
                     RLEMHKSSHDFIQEKAKGIFIIQSCAKDHVYELDLRDAVYNVEAPWGRAGNEYIRIDI
                     IKGVGSIREYALVPCESDTKNTKFFDFTQWNQNYTYTGLESRYCKARDLASYILWSAF
                     VRPEGKLTRNSVYSSKNWMTNIWSWDNCFCALAMVESMPDLALDQLLVFADNQHKAGA
                     YPDCINDRFSLFSFCKPPIHGWTLLEMIKRNPTILQSSSESIEYLYNSISESTNFWLR
                     FRRLPSGAIIYRHGNDSGWDNATLFMHGGPVESPDLYAYLSRQCDSLAEISRILEHGI
                     EEAQNWTFLAESLAESMLNRLWDDNGFFARKGLLGERIEHRDSFLLRLPIVANKRLPE
                     EVIKKLISDISDESRFLTPFGLATESLDSSFMEPHGYWRGPIWAPVNYLVFDALRLLG
                     ELNLANRIAQRFVDLCAQKGMAENFDPIHGEGLDDPAFAWTSAVFLLFLKESI"
     gene            8595..9587
                     /locus_tag="PK105_04295"
     CDS             8595..9587
                     /locus_tag="PK105_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU60961.1"
                     /translation="MNKTAIRADLLLLLTAIIWGFAFVAQRVGMNYVGPFTFNAIRFA
                     LGSVSLLPLIMYARKRNRKNGSRGLDSSEELDRSGEAGCEQRSMLNEDQTRATSKQKL
                     FLSGSFVAGSFLFIAASLQQIGIQYTTAGKAGFLTGLYVVLVPIVGMVWGRSTGLPTW
                     VGAILALVGIYTLSAPDKLGRVNSGDIFVLISAFFWTFHVLVIDNLAKRLDPVELAAG
                     QFAWCSVFSFVAAMIFEKISSHAVLDALVPILYGGLGSVGVGYTLQVLAQRDAPPAHS
                     SIIMCLEGVFATLGGILLLSESPSLRTMLGSVLMLLGMLATQWDVIFSGRSKQA"
     gene            9773..11005
                     /gene="corA"
                     /locus_tag="PK105_04300"
     CDS             9773..11005
                     /gene="corA"
                     /locus_tag="PK105_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOU60962.1"
                     /translation="MFSKKQNSAEPNTVCRASSEVGEGMAFEQSVFEKKASRESAQSN
                     NEKAKAAKPKHHASKLPFSGVSVETAGLPPGTPHYIGDIEPTLASYSLYSYDETSAVM
                     SIPDDISELLALIDLDVVNWININGLSGGIVEKLCGMLHIHPLVIEDILNTEHRPKLE
                     NYDDYLFLITKMITAHEDGSVEYDQVSFILKGKLLISFQERPGDCFKAVRDRIMSGAG
                     RLRKHGADYLLYALLDSIVDNYFIVLEMLGDMLERFEEASDSESDQEFFHGIQQLKRE
                     FSRMRRIIWPVRDSIAALSRTESIQMDVSLSPYLRDLYENTVQAIEALESYREHATSM
                     IELHLSQVNTNMSRVMTLLTILSAIFIPITFIAGIYGMNFAYMPELNKPWGYPAALLL
                     MAAVVIAELIYFKKKKWM"
     gene            complement(11020..12393)
                     /locus_tag="PK105_04305"
     CDS             complement(11020..12393)
                     /locus_tag="PK105_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOU60963.1"
                     /translation="MIVDIEEFGGIDDGCTDNSKAFADAMAALSETGGGTLRIMGSAY
                     LTGPIELLSNISLEIGKNTTLSFVSDINRYPPVLTRWEGVVCYGMHPLLFARGAKNIE
                     LKGEGTIDGNGQPWWEALRRKKRKGQNKPLESFETELAELNKYNGGQPSGGGGREIQF
                     LRPPLVQFFGCSDVAIEGLKFQNSPFWTIHPVFSNRISIRNISIHNPSDAPNTDGIDI
                     DSCKDILVTGSTIDVGDDCLAVKAGSGEQGISEGKSSERIVIRDCHFKQGHGGVVIGS
                     ETAGGIYEILVSDCSFDGTDRGVRIKTRRGRGGTVSRLRFSDLHMKRALCPVAINMYY
                     RCGAKLEECSKLFSLEHQPVDALTPHIHDIEISNLRAQECRASAGFIIGLPESPIENL
                     RMRNCRIELSRNDMVAPSESEMFEGLPETQARGIRIRNANCDFDGLEVLGLPEGEAPY
                     FREPSPN"
     gene            12549..>15840
                     /locus_tag="PK105_04310"
     CDS             12549..>15840
                     /locus_tag="PK105_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU60964.1"
                     /translation="MQGHYQRIALLLASAHTGAARGLWPELIAAAQSHRCILFVLPGG
                     RLDAVSGFEYMRNGVYRYAKASNVDAALCWASTLSGYATESAVESFLNDTIDVPLVTF
                     GLKIDGRPFVGIDAYSGMKALIGHFIRSHKRKHIAFIAGPRQHSSAEERYRAYCDALK
                     EASIPYDPRLVCLDTPWSEGRRAMLNLIDDRGLVPGKDFDALCTSSDLLAFEAGMLLK
                     ERGIDIPSTLPIGGFNDSEESRIFSPPLTTVRMPFIRQVLSALRLLIDMRNGNSADDI
                     LLKTKLLVRSSCGCMPQALQLAGRTRAKAPKSLPPSLIEAFGASVASQKNNSFFEYLN
                     KIFNEDRASSSLETGISENMMTALRFEFLSRASSREDIDKTERLIHQAMVALGLSKER
                     RHEYLLWKEKEDEQKLNLFNQELLCAKDVLSIIRAAAGHLPGLGILSSYLVTKDKQGN
                     LMFKGGYRKGKDTPYAELIYPRPSREAIPRGCFLPDRIIPTEPGAFFVLPLFFESTEI
                     GHLVIQAADIDPSLYEKIRSILSSALRGVKLFEEGDEARKRAERAEKSMSDIVSVISR
                     EIQSSIEAFDNISKLDATGAQEVEVSRLKELVRYLIDLSQAKSESVLARAVLFDPYCL
                     CARWAEEQLKADKSNNKGSNRAESRFICARPSSAYPLLYGDPKQLERMLTIVAQLLEE
                     TFSIEGLEISIVPSIQSCELRLASASMIPKKIGATGLNRIEKDIRFELAKQICLLHGG
                     YILFSHEGGNLIVRIGIPYPTVSGSIHNISNREAELRVIGPQGLSDHLPCGFVNGLSG
                     KIIPYNSIFHESINPLQRILLYLDSMEIDHESSAALSFILDKEKFQNAECFIAQNAFS
                     IRETRLEHRPADVAGFLRSILVSDSPSAILVLGSEASLHKETPIIRDLVQKPGIRPVA
                     CTSPDAFEAALNRWRPMLFLILEPMISKNQEFSTLLQRFPSIPIMAICRFFGAKEEWA
                     GIIERPHVVFCNTGEVFSEVLAGLIQRSLESKKFLPSPTSGVVADAIFYMNKSYSEQI
                     SRWKMASYLNASEDYLSRIFHAQMGISLWNYLARLRVQHAIDLLYSTGAGLAEIADLT
                     GFQDEAYFC"
BASE COUNT         4287 a         3420 c         3747 g         4386 t
ORIGIN      
        1 gggatagctc aggataggtt tcttgaaaag gcagaaaatc ggcgtaacgc tgccagattg
       61 aggcgtcaga agcggctgca ttttcattct gggtgcgaat caatgtgcct cgagccatag
      121 gctctcctag cgtgcccgct ttgcgttttg agctagcaca aatacggtca catataccag
      181 tcgatgtcga gagtggccga gcatgttcaa tattagggat aaccacttcg agcgattcgc
      241 cattttcggc aaattcattc agctgttgga aaggatagct agccccggat agagtcgaga
      301 ttagatgtgg tttcagcatt gatcctcctt tgaagggtgc actatagagt aatactaaat
      361 ccttgattag tgctacgaac cttgtgtaag ataataaagc agaaatgcgg caaagagagc
      421 aaataagaaa taaataagtc gaaggaaata atattgatct tccttagttg tatttagcta
      481 taagtgagga gatttcttac tatcagccta acccatacac aaggttcgta tcagtaaaaa
      541 agattaggca agaactcagt aaaccatgga acataagtca caagaaataa cactgcaagc
      601 atcgccggcc agagtggaag cattgccttg cttgcttttt cgattgttgt tttgccaatc
      661 atgcagcctg taaagagact tgctccgacc ggaggcgtgg taagcccgat acccaggttg
      721 agcatgagca ctatgccaaa ggttatctcg ctcattccag ctgccttgac aacaggcagc
      781 aatacgggcg tcacaattac gataagtggc gccacgtcca tcaccatgcc taacaaaaga
      841 agaaggacat cgacgagcag aagtattagg aagcgattat ttgtcagact caggaagaaa
      901 ttcattacag caagaggtat ctgtaggtag gtcatcatcc aaccaaaggc cgaagcgcaa
      961 cctatgagga aaaaaacaac agaggttgtt ttgaccgaat ccatgaaaat tggccacaaa
     1021 tccttcagct tgagttcctt gaagataaag aagcctaaga aagccgcata cagaactgct
     1081 accgcagatg cttcggttgc agtaaaaatg cctgccgcaa ttcccactat tataatagca
     1141 gcgcagagga gggcgagaat tccatctaag atgatgcgca ggctctcttt aaggggaggt
     1201 cgtttttcgc atggataatt tcgctttagc gcaataaaat agcatgcaat ggcaagcgca
     1261 atgcccaaca gcattcctgg aatataaccg gctaagaaga gtctcccaac agagactcca
     1321 cctgctacca ttgcatatat caccatatta tgggaaggtg gaatgattat gccctggctt
     1381 gccgaagcca ctgtaagcgc taccgcgaag tccttatcat agcctttttc caccatcatc
     1441 ggtattatca ttggtccggt tgaggataca tcggccactg ccgatccaga aactccaccg
     1501 aaaaacatcg aatcgataca gttgactatg gcaagaccac caggcaaagg gcccacgatt
     1561 acataggcca aatccaccat tcgcttggct actcctccgt tggccatcaa tgctccggcc
     1621 aggataaaaa gaggaatcgc aagaagggga aacgattgca tgcctgcggc tacgcgctga
     1681 gctataatag ccggctgcag acctgcggca aatgcagaaa gcagggatga aatgcccaaa
     1741 ccgaaagaaa ttggtacacc aagcaagata aggattataa aggatcccaa cagcacagat
     1801 tcagcgagca tcttcgcctc cgtttttggt ttcttgttgg ctagatattg catttgctcc
     1861 gcttgccttc tttacaatct tgagaataga aaagagaatc atcatggcag ccgagatcgg
     1921 gatgaccagg tatgtaatac caatagaaag tcgaatggtg gataattggc tcgtccacat
     1981 tgcgtttaca agttcaatac cttgcttgaa taagaaatag gagaaataga tcactgctcc
     2041 atcgatcaat atctctaaaa tcaatccaaa acgagcaggc aggtgtttgg tgagcaattc
     2101 aagtctcatg tggtcgccag tccatactgc gcaggcggca ccgaggagcc ccatccacat
     2161 cattatggtt accgagaatt cctcagccca atagggactt ttatgcaata cataacgatc
     2221 gaagacctgc cagcacacca caatggtcac tatccccatt aaaatggcaa taaatccagt
     2281 taatatccgt tctaatctat cgacaatgtg ccaaaggtct tttcgcatat cgccttcctg
     2341 atgatcctgc attagaacaa tcctttctag ttttgaaaat gggcggtaga atatctgccg
     2401 cccatttttt tcctgtcatg atagacagga agtatttagc atctaagcaa cagcgccatc
     2461 tattttgtat tgactatagc gtctatcaaa tcttttccta cgatcggctc aaactccttc
     2521 cacactccgg cgcatgcttt ttggaattcg gtgatgtcct tgacttcatt gaagatcatg
     2581 cctttagctt ttagttcagc gacattctta tcgatcattt cgccccagag ctgattgtgc
     2641 ttttgcattg cttcgaaggc tgtctcttcg atcatagtct ggatgttctt gggtagacca
     2701 tcgaagagtt ttttattcat tatgaggata tcgggaatag aggaatggcg cgtataagag
     2761 aaatatttag caacttcctg catgttgaag gatagcactg tgggctcatt gttttcccag
     2821 ccatcaatga catttgtctg cagcgcgctg tagacttcag attgacccat tggaacggct
     2881 gttgcaccca atgcgtttat tgtatcgata aggggcttgg actgcatgac gcgaatcttt
     2941 aggcctttga gatcggcagg ggtgtagatg ggcttctttg cagtaataga tctaaaacct
     3001 gcatccataa agactagccc cttgaagcct gccttatcga gatcattcat caatctcttg
     3061 ccaacttgtc cttgcaacac tttatgctga tgatccaggt ctctccagat tagaggcaga
     3121 gagacaacct gaagtgttgg ggcatattgt ccaagtgggc ctgatgagac cttggaaaat
     3181 tccaacgatc caatctttat agcctgaatg taatccgctt cttgacccaa ctggccggca
     3241 ggaaagagag tgactttgat atcaccattg ctctttcgtg ctaattcgtc agcaaagaaa
     3301 accagagcct gatgaaccgg atgtgagtcg ggcagtactg acgcaagctt ccagttgtat
     3361 tttggagcgg caatggcgaa gcttgccgta atgagcaaaa gcgctgccag agcgattagt
     3421 tttttcttca taacaaaccc cctataattg atctttatgc ttattcagcc cttaggtcgg
     3481 tcttaaggcg actaaggcca aacaatatcc catagtctgg aatacattct cagaatgccg
     3541 caaaccatga gattcatcta tagaattcca caatatggaa tccaatttca tatgataaat
     3601 catcttttga acttgtcaag tgtgattcaa gagaatctcg ataaaaaata agcggaatcc
     3661 attattgaat cccgatccat ttacagagag tcatgcggcg agcacagccg acccagttcg
     3721 attgcctgtc atcttaagat cattcaactt gcgtggattc tagctcgcat ctatactagc
     3781 aatgagcttt ggctatattc atcttatgga gtcacttatg aacagtctga aagggaaaaa
     3841 agctctggta actggaggag catctggtat aggtcgcgct atttctctgg aattaagcaa
     3901 tatgggggct gatctcatta ttcattatca caagagcaga gagttggccg aaagcctagc
     3961 agagcaaata cgcacttcag gtggcaaggc agatgtggtg caagcggatc tttcggaaga
     4021 aaaaggtgtg cagatcctag cggatttcat atttgagaga tggaataatc tcgatgtgct
     4081 tatcaacaat gcaggtgacc tggttggacg ctatagactc gaagatgttc ggatggattt
     4141 ttaccgaaaa gtcgtggcag taaatctaga ctcaaatgtc ttggtcacgc gagccatgat
     4201 acctttattg aaaaggtcgg gctgcgcctc aatagtgaac cttgcgtcgc tggctggacg
     4261 caagggtggc catggtggtt cgatagtcta ttctatgaca aaaggagcca ttcttaccct
     4321 tacacgtgcg ctctccgttg agcttgcacc ctacgggatt agggtaaatg ctgtggcacc
     4381 tggttttatt cttggttcgg cttttcatgc cattcacaca agcgaagaat ccaagcaggc
     4441 aacgatacga gatatacctt taaagagggc tggtacatgc gaggatgtcg ctcgtgcggt
     4501 ggcttttctt gccagtgaat acgatggatt cattaccggc gccacattag atatcaacgg
     4561 tggtgtctat atggcatgag agcttccgta tttttcgatg cacgcgaaaa ataaaaaatt
     4621 atttatgggc tcgatcttaa tactccacta gataaaactc ttgcgaaaca ccacgtctgt
     4681 aggcaccatt actttaaccc cgagctcacg aaaatccgca gctcgcgatg atgccagaga
     4741 tagcgcaact gagcccatta ctgccctcgg aattcttatc gtgctcaatg gaggtgtcat
     4801 atatgccgat acgggttgat cattgcagct gataactttt atatcctgcg gaatcgaaag
     4861 attcgcttca caaattgcct tatagacacc tagagccata ttatcgctgg caacaaatat
     4921 agcctctggt ttggttgtgc cggataaaag ctccttagtg aattgatacg cattttcagg
     4981 ggtatatggc ccgtaagctc taaagatttc ctttctatag atacccagtt tcccaaattc
     5041 ctctatatat tgtgcgcttc tagggtcgat tatttctttc cccttgagag gagagatttc
     5101 tttgcctccg atgtaaccaa tacgcgaaat acccagctca gcaaatctct ttgcgattgt
     5161 tccgatagat tttccgaaat ctgcacatac aacatctatg tttttcaaat caggatcgaa
     5221 gtcgatgcat acagcgttct tttctttctt tagaaaattt agcaatgata tatcgcagcc
     5281 accctcgctg ccgataatga tcgttgccga tctatcaaaa gagagcaatg caaggtcatt
     5341 tctattacta tagtaaatgt catctattcg tattgctcgt cttgccgccg ccattttaag
     5401 ggcatgcacg aaatgcaggt tattccgatt atggctataa ccgcgattat gataatacca
     5461 cctatcatcg catttgcatt tgcacagaag cacataatag aaggcatgag tggagcactg
     5521 aaaggataga gcatgaacac atcatatggt gcagaatgcg actggcagaa tcacaagctt
     5581 ctccatatag atcggctacc tggacgatca tacataaaac gatggaaaga tgtgcaagga
     5641 gccttacgag acgatgaatc agaacaactg gggtttcgga acttgaatgg atcatggaaa
     5701 ttcctttttc ttgaggctcc tgagctttct ccttttggat tcgaaaagcc tgatttcgat
     5761 gacggcgctt gggaagatat cgaggtgcca tcgcattggc agctaaaagg tcatggaagg
     5821 ccacattata cagacctata ttatccattt cccgtgaggc cgccttttgt cccgagagca
     5881 aatccaaccg gaatatatcg gcgtcagttt tttttggatg atctgtcgcg tcgtcagatt
     5941 ttgagatttc atggtgtgga ttctgcattc catgtttgga taaatggaaa gctggcaggg
     6001 ttttctaaag gttcgcgcct gttgtcggaa ttcgatatca cttctttggt tagagttggc
     6061 aaaaacgtaa ttgtggtaag agtatatcag tggtctgatg gaagctatct ggaagatcag
     6121 gatatgtggt ggctctcagg aatattcaga gatgtcgaga tatacgaagt cccacttgtt
     6181 tcgttgttcg atgtgtcaca tgaggtatcc ttcgatgata tatataagaa tgcacttcta
     6241 aaggtcgaat tgcagataga aaacaaaacc gaagatatgt caccaggcgg acgatgcttc
     6301 aagatgttct tctgatgaag cagcataata tcaacgctgt ccgtacttcg cactatccca
     6361 atcatcccta ttttctcgag ttgtgcgatt attttggcct ttatgtcata gacgaaaccg
     6421 atctcgagac ccatggattc gagtggatcg atgagagcac aaggcttgtg aacgatcctg
     6481 aatacaatcc catatataag accgcaggag aatggcaata gatatggcac tgatgcaatt
     6541 tcgattacag atagacaagg ttttggatta gcgataattt ccgaaaagcc aatcgaattc
     6601 tcttatcatg attatgatgt agatgcattg gaaaaagcgt gttatgatca tgaaattgct
     6661 catactgctt attgtattct gaatctagat tttgcgcaaa atggtatcgg cagcaattca
     6721 tgtggtcaag atcagcttcc tccatataga ttgaagcctc aggaattcga tctaggcctt
     6781 gaattctacg cactcgatcc ggaaacttcg tttctggcaa atgcaaaatc tattggagaa
     6841 tcttgaaaaa tggatctcga tatttcgaaa acgccttggt cggcttttgg tgcgcgtttc
     6901 tctgttgcga tcggaaaaga tggttcggtc tatttgaaag atatcggagg gggagacgag
     6961 attccgagtg agctattctc gatgaagctt ctcttgggca aagaagggct cgatcaaaaa
     7021 gaagaactcg aacaagtgat agaggcaaat gagaacgaat gccggattcg cgctaagaat
     7081 tcgcctgatc gctatgttct tctgaaattc ctgttttccg atgtaataag tattgaatgc
     7141 tgtgatatac gtcttaggct ggaaatgcat aaatcaagcc atgatttcat tcaggagaag
     7201 gctaagggga tattcataat tcagagttgc gcaaaagatc atgtctacga actcgacttg
     7261 agagatgcag tttataatgt agaggcccca tggggaaggg ccggcaatga atatattcgg
     7321 atcgatatta tcaaaggagt aggcagtatt cgagagtatg ccttggttcc ttgtgaatcg
     7381 gacaccaaaa acaccaaatt tttcgacttt acccaatgga atcagaatta tacctacaca
     7441 gggcttgaat ctcgatattg taaggctaga gatctggcat catatatcct ttggtccgct
     7501 tttgtgaggc ctgagggaaa gctcacgcgc aactctgtat atagctccaa gaattggatg
     7561 acgaatatct ggagctggga caactgtttt tgtgcgcttg cgatggtgga aagcatgccc
     7621 gaccttgctt tggaccaatt attggtgttc gctgataacc agcataaagc tggtgcctat
     7681 cctgattgca tcaacgatcg cttctcgtta ttcagctttt gcaagcctcc cattcatgga
     7741 tggactttgc ttgaaatgat aaagagaaat ccaaccatac ttcagtcttc cagcgaaagc
     7801 attgaatatc tttacaactc gatttccgaa tcgaccaatt tctggctcag atttcgcaga
     7861 ttgccttctg gagccattat ctatcgacat ggcaatgatt ctggctggga caatgcaacg
     7921 ctgtttatgc atggaggacc tgtcgaatct cctgatctat acgcgtatct ctcacgacag
     7981 tgtgattctc ttgctgagat atcgcgtatt ctggagcatg gtatcgaaga agcgcagaac
     8041 tggacatttt tagcagaatc gttggctgaa tcgatgctga atcgcctttg ggacgataat
     8101 gggttctttg cacgaaaagg gctcttggga gaaagaatag aacacagaga ctcttttctt
     8161 cttcgcttgc cgatagttgc gaacaagcgc ttgccagaag aagtaatcaa aaagctcatt
     8221 tcagatattt cggatgaaag tagatttttg acaccttttg gcttggctac cgaatcattg
     8281 gattcttctt ttatggagcc tcatggatac tggagaggcc cgatatgggc gccggtgaat
     8341 taccttgtat tcgatgcact taggctgcta ggagaattga acttggcgaa tagaattgcg
     8401 caacggtttg tcgatctatg tgcacagaag ggaatggctg agaacttcga ccctatccat
     8461 ggcgagggat tggacgatcc agctttcgct tggacatcag cggtctttct tctttttctc
     8521 aaagagagta tatgaaaggg catagggaat tagccctgga gtcgagctag ttctgcttag
     8581 aggaatccaa taccatgaat aagaccgcaa ttcgagctga tttattgctt ttgctgacgg
     8641 caattatctg gggttttgca tttgttgccc agcgagtggg aatgaattat gtggggcctt
     8701 ttactttcaa tgcgattcgc tttgcattgg ggagcgtttc attgcttcca ttgataatgt
     8761 atgcccgcaa acgtaatcgc aaaaatggat cgagaggctt ggatagttct gaggaattgg
     8821 acaggtctgg ggaagccggg tgcgagcaga ggagcatgct gaatgaagac caaactcgcg
     8881 caactagcaa gcaaaagctc ttcttgtctg gttcttttgt agcggggagt tttcttttta
     8941 ttgctgcttc tttgcagcag ataggcattc aatacacaac ggcaggaaaa gcggggtttc
     9001 tgactggact atatgtggtg ctggtcccaa ttgtgggaat ggtctgggga agatctacag
     9061 gtttgcccac ctgggttggg gctattcttg cactagtagg tatttatact ctgagcgcac
     9121 cagataagct tggaagagtc aactcgggtg atattttcgt tctgataagc gcttttttct
     9181 ggacatttca tgtattggtt atagacaact tggccaagcg attggatcca gtcgagctgg
     9241 ccgctggcca gtttgcctgg tgctcagttt tttctttcgt agctgcaatg atttttgaga
     9301 aaatatcgtc tcatgccgtc ttagatgcgc tggttcctat tctatatggt gggttgggat
     9361 ctgtaggagt aggttatact cttcaggtat tggctcaaag agatgcaccg ccagctcatt
     9421 catcgatcat aatgtgtctg gaaggagtat tcgctaccct tgggggtata cttcttttat
     9481 cagaaagtcc gagcttgcgc acaatgcttg ggtcggtcct tatgctgctt ggaatgcttg
     9541 cgacacaatg ggatgtgatt ttctccggtc gatcaaagca agcatagtac tgttcttgag
     9601 tgataaaaca atgacaaata gcagcgcaca aaggacataa tttgcaggat cggcaaaaca
     9661 agtatcgatg aagaaagctt agcttagatg agcgatatga agaccatatc cagtcagggt
     9721 ttttccgggc ttgcttccat gcccccgaat agctagtcta taagatatga agatgttttc
     9781 caagaagcag aatagtgccg aacccaacac agtatgcaga gcttcttccg aagttggtga
     9841 aggcatggct tttgagcaga gtgtgttcga aaaaaaggcc tcacgcgaaa gtgcgcaatc
     9901 aaacaacgaa aaagcaaaag ctgcaaaacc taagcaccat gcttccaagc tgccatttag
     9961 cggtgtctcg gtggaaactg ctgggcttcc accaggcact ccacactaca ttggcgatat
    10021 agagcctaca ttagcgagtt attcattgta ttcctacgat gagacaagcg ctgtaatgag
    10081 tattccggat gatataagcg agcttctggc tctcatcgat ctagatgtgg tgaattggat
    10141 caacatcaac gggctttcgg gaggaatcgt tgaaaagctc tgtggcatgc tgcacattca
    10201 cccactggta atcgaagata ttttgaatac agaacatagg ccaaagctcg aaaactatga
    10261 tgattatctt tttctgataa caaagatgat aacagctcac gaagacggct cggttgaata
    10321 cgaccaggtc agttttatcc tcaaaggcaa attgctgatt tcctttcagg aaaggccggg
    10381 cgattgcttt aaggctgtgc gcgaccggat aatgtctgga gcagggaggc tgcgaaagca
    10441 tggcgcggat tatctgctgt acgcgctatt ggactcgatt gtcgataatt actttattgt
    10501 gctagaaatg ttaggagata tgctcgagcg attcgaggag gcgtccgatt ccgaaagcga
    10561 ccaggagttt tttcatggca ttcagcaatt aaagcgggaa ttttcaagga tgcgtcgaat
    10621 catctggcct gttcgcgatt ctatcgccgc gctttctcgt acggaatcca tccagatgga
    10681 tgtctctcta tctccttatt tgcgtgatct ttatgagaat acagtgcagg caattgaggc
    10741 gctcgagtca taccgagagc atgccacttc aatgatagaa ttgcatctga gccaggtaaa
    10801 tacaaatatg tcgcgagtaa tgacactact cacaatattg tcggctattt tcattccgat
    10861 aactttcata gcaggtatct atggaatgaa ctttgcctat atgcccgaat tgaacaaacc
    10921 ctggggatat cccgctgctc tattgcttat ggccgcagtg gtaatagcgg agttgatcta
    10981 tttcaagaag aaaaaatgga tgtgaaatcc cgggctcttc taattaggtg agggttcgcg
    11041 gaaataggga gcttctccct ctggaaggcc aagaacctcg agcccatcga aatcacagtt
    11101 tgcattcctt attctaattc cgcgcgcttg cgtttcaggc aagccttcga acatttcaga
    11161 ttctgatgga gcaaccatat catttctgct gagctcgatc ctacagttgc gcatgcgtag
    11221 gttctctatt ggcgattctg gcaagccaat aatgaatcca gcggaggcgc ggcattcttg
    11281 ggctcttagg ttgcttattt cgatgtcatg gatgtgggga gtaagagcat caacaggctg
    11341 gtgttccagc gaaaagagct tcgagcattc ctctagttta gctccgcagc gataatacat
    11401 gtttatagca actgggcata gtgctcgttt catatgcagg tcggagaatc taagcctgct
    11461 gaccgtccct cctctacctc gcctggtctt aatccgaaca cctcggtctg ttccgtcgaa
    11521 ggagcaatcg cttacaagga tctcatagat ccctcctgcc gtttcgctgc ctatgacaac
    11581 accgccatgt ccttgcttaa aatgacaatc gcgaattaca atgcgctctg aagattttcc
    11641 ttcgctaatt ccctgttctc ctgagcccgc cttaactgca agacaatcgt ctccaacgtc
    11701 gattgtggaa cctgttacca agatgtcttt gcatgagtca atatcgatac catccgtatt
    11761 tggcgcatca gaagggttat gtattgagat attgcgaatg cttatgcgat tcgagaatac
    11821 aggatgaatt gtccaaaatg gggagttttg gaacttgagg ccttcgatag cgacatccga
    11881 acacccgaaa aattggacta gtggaggtcg caaaaattga atttctctgc ccccgcctcc
    11941 tgagggctgg ccaccattat atttattaag ctctgccagc tctgtttcga aggattcaag
    12001 gggtttgttc tgtccttttc tctttttgcg cctcagggct tcccaccaag gctgaccatt
    12061 tccatcgatg gttccttcac ctttgagttc tatgtttttg gctcctcttg caaataaaag
    12121 agggtgcatg ccatagcaga caaccccttc ccagcgagtg agaactggag gataacggtt
    12181 tatgtcggaa acgaagctca atgtggtgtt cttaccaatt tctaagctga tattgcttag
    12241 aagttctatc ggacctgtga gatatgcgct gcccattatt ctgagcgttc cgccgcctgt
    12301 ctcgcttaag gcagccatcg catcggcaaa tgcctttgag ttgtctgtgc atccgtcgtc
    12361 gatcccgcca aattcttcga tatccactat catttagttt cctcttctat aattgagatt
    12421 tatagaggag ttttgaagag tcttcaattg cgtatgttgg ctgttaattg gacttttctg
    12481 tctttttgta agcaaaaaag aataaaatgt tgacaactcc cataagatga gctcacaatc
    12541 gataaagaat gcagggacat tatcaaagaa tagctcttct tcttgcatct gcacatactg
    12601 gtgctgcgcg aggtctatgg ccagaactca ttgccgccgc tcaatctcat cgatgtattc
    12661 tctttgtgct gccgggtgga aggctagatg ccgttagcgg ctttgagtac atgaggaatg
    12721 gcgtatatcg ctatgcaaag gcatcgaacg tcgatgctgc cttgtgctgg gcttccaccc
    12781 tgagcggata tgcgacagaa tcggccgtcg agagctttct taacgatacg atcgatgttc
    12841 cattagtgac ttttgggctt aaaatcgatg gacggccttt cgtcggaata gatgcatatt
    12901 ccggtatgaa agcccttata gggcatttta ttcgaagcca taaaagaaaa catattgctt
    12961 ttatcgcggg tcctcgacag cattcttccg ccgaagaaag ataccgtgcc tattgtgatg
    13021 ctctgaagga agcgagcata ccctatgatc ccaggcttgt atgcctcgat actccatgga
    13081 gcgaaggtcg ccgcgcaatg ctcaatttga tcgatgaccg cggccttgtc ccaggtaagg
    13141 atttcgatgc gctatgtacg tcgagcgatc ttctggcatt cgaggctgga atgttgctca
    13201 aagaacgagg catagacatt ccttcgaccc tgcccatagg cggattcaat gactcggaag
    13261 agagccgcat tttttcgcct ccgcttacta cggtgaggat gccttttatc cgccaagtgc
    13321 tttccgccct aaggcttctg atagacatgc gcaatgggaa ttccgcggat gatattttac
    13381 ttaagacaaa actcctggta cgcagttctt gcggatgcat gccgcaagct ttacaactag
    13441 cgggtaggac aagagctaaa gcgccaaaat cacttccacc tagccttata gaggcattcg
    13501 gtgcttctgt cgcctctcaa aagaacaatt ccttttttga gtatctaaat aaaatattta
    13561 atgaggatag agcatcgtct tctttggaga ctggcatatc agagaacatg atgacagcac
    13621 ttcgcttcga atttttaagc cgggcttctt ccagagaaga tatagataaa acagagagac
    13681 taattcacca ggcaatggtt gcgttggggc tctcaaaaga aagaagacat gaatacctcc
    13741 tatggaagga gaaagaggac gaacagaaac tcaatctctt caatcaagag ctgctctgtg
    13801 caaaagatgt tctgtcgata ataagagccg ccgctggaca tctacccggt ttgggaattc
    13861 tctcgagtta tcttgtgact aaggataagc aggggaactt gatgttcaaa ggcggttata
    13921 gaaaaggcaa ggatactcct tatgcagaat tgatatatcc gaggccctcg cgggaagcca
    13981 tccctagagg ttgctttctg ccggatagaa ttattccgac tgagccggga gctttttttg
    14041 tcttgccttt gttctttgaa tcgaccgaga ttggccattt ggttattcag gccgccgata
    14101 tagacccttc actctatgag aaaattcgct cgattttatc ttcggcactg agaggagtga
    14161 agttgtttga agaaggcgat gaagcaagaa agcgcgccga gagagcggag aaatccatgt
    14221 cggatatagt ctccgtaata tccagggaaa tccagagctc gattgaggca tttgataata
    14281 tttcgaagct cgatgcaaca ggcgctcaag aagttgaagt atcgagactc aaagaacttg
    14341 tgcgttattt gatcgatctt tctcaggcaa aatcggaatc cgtgcttgcc cgggcagtgc
    14401 tttttgaccc ttattgcctt tgtgctcggt gggctgaaga acaattgaaa gccgataagt
    14461 cgaacaacaa gggaagcaat agggcagaat ctagattcat atgcgctaga ccttcttctg
    14521 catacccgct tctatatggt gatcctaaac agcttgaacg aatgctgacg atagtggctc
    14581 aattactgga agagaccttc agcatcgaag gactggaaat ctcgattgtg ccatcgatac
    14641 agagttgcga attgaggctg gcctcggcat ctatgattcc gaagaaaatt ggtgctaccg
    14701 gattgaatcg gatcgaaaag gatatccgtt ttgagctagc gaagcagatt tgtctgcttc
    14761 atggaggtta tattctattc tctcatgaag ggggtaatct tattgtgagg ataggcattc
    14821 cttatcccac cgtaagcgga tccatacata atatttcgaa tagagaggct gagcttcgtg
    14881 tcataggacc acaaggcttg tcggatcatt tgccctgtgg attcgtgaat ggattatctg
    14941 ggaagataat tccctataac tctatatttc atgagtcaat aaatccttta cagagaatat
    15001 tgctatacct ggattccatg gagatcgatc atgaaagctc cgcggctctc tctttcatac
    15061 tcgataagga gaagttccag aatgccgaat gtttcatagc ccagaatgct ttttctataa
    15121 gggagactcg cttggagcat cggcctgcgg atgtggctgg attcttgcga tcgatacttg
    15181 tgtcggattc tccttcggcc attttggttc tgggaagcga agccagcctt cataaagaaa
    15241 cacccattat tcgagatctt gttcaaaaac ctggaataag gccggttgcg tgtacatctc
    15301 cggatgcctt cgaagcagcc ttgaatcgct ggagacccat gctttttcta atcctagagc
    15361 cgatgatatc gaaaaaccaa gagttcagca cacttttaca gcgatttcct tccataccga
    15421 tcatggctat ttgcagattt ttcggggcaa aagaggaatg ggctggaatt atcgagcgcc
    15481 cccatgtggt tttttgcaac acgggcgaag tattctctga agttttggca ggcctgattc
    15541 aaagatcact ggagagcaaa aaattcctgc caagtccaac aagtggcgta gtcgctgatg
    15601 ccatattcta tatgaataag tcgtatagcg aacagatttc gcgatggaag atggcttctt
    15661 atctcaacgc gagcgaagat tatctcagca gaatatttca tgcgcagatg ggaatatcgc
    15721 tctggaacta ccttgcaagg ctaagggtcc agcacgccat cgaccttctg tattctacgg
    15781 gagctgggct tgcagagatt gcagatttga ccggtttcca ggacgaagca tatttctgca
//
LOCUS       DAOOWY010000036        15779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3736_length_15779_cov_11.922094, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000036 DAOOWY010000000
VERSION     DAOOWY010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15779
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3736_length_15779_cov_11.922094"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1472)
                     /locus_tag="PK105_04315"
     CDS             complement(15..1472)
                     /locus_tag="PK105_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOU60965.1"
                     /translation="MHFKSILRMLSIILLIVSIFSLICGIYSIVEDLSWRVSFSLFIP
                     TIISLAFFIVMYLMTRHDERPFLTNKDSFLFVAFSWVFSSALGAIPFTFSGYIPSYID
                     AFFETVSGFTTTGASILTDIEIIPRPLLLWRATTHWLGGMGIVVLLVAILPSLGFSAI
                     RIMEAESPSPEVDRITPRISKTAKTLWLIYCGFTVCEIILLVLGGMPLFDAICHSFAT
                     MATGGFGTKNASIAYYKGGFIQWVITIFMFLAGTNFTLHYHFLKGRMSTILLDTEFRV
                     YLGIFAISSVIIAVDLFVSGVFVNIGDSLRHASFQVSSILTTTGFASTDFGQWPQLSQ
                     TVLFFLMFIGGCSGSTGGGVKVIRLVVLFKMAVNEMRYVTNPRGVYTIFLGNRALRKN
                     VIYDISGLVFLYFAFFFLSVLVIAFSGVDLLTNITAVIANLGNIGPGLGKVGPAFNYA
                     LFPWWAKLWLSLDMLVGRLEVYTVLALFTRKFWRA"
     gene            complement(1474..3045)
                     /locus_tag="PK105_04320"
     CDS             complement(1474..3045)
                     /locus_tag="PK105_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOU60966.1"
                     /translation="MQNANEAQYVDVDAPIYRAFSIFGAAQLLGIQQALHPSPVIVKI
                     ALGRIYFSSMKIAILGAGLLGSLIASELIAENKDVVIIEKNLNAAKSIADKLDCIVQE
                     GDGENIETLKAAGVEDADWFIACTGSDEANIVSCGLVAENFKDVRTIARARNPYFASF
                     KNAGKKILGVDYIINPEAETAESIAGTIFRGMSPEIIDVKEAGIQLRRILCKKEPRFV
                     HQSLANIRTSIGADFMVPAVLREEELIVPGGDFVFEEEDAAYILGEPSSLDRLFGPCK
                     DTLRKFSSIVILGTGTLTTLLLKELGMRQISAFSGSQRDKGYDRGALSLLGNPKIKVI
                     YGDRESLKILSATFPDIEAINHQLSDEHLIREENIGSADIVLCLASEQSINILSALLA
                     QEAGAKRSLALITNDLYTPLLYLLDINIVVNEKIVMSGAILDKVRKAKIRRLYSFPHN
                     EYELIEIQITDSFEQLEVEIRNMNLPKGLLICFVIHRGKTCIPTGSTKIFENDLVGLI
                     IKKEHIGKLESIFGA"
     gene            3070..4389
                     /locus_tag="PK105_04325"
     CDS             3070..4389
                     /locus_tag="PK105_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOU60967.1"
                     /translation="MRNRLGKEKQIPLLYFLGTILAGSFLLVLPISSEEGNLSYLDAL
                     FEATSAVCVTGLAVVDTTRLSRFGQTVILFLIQLGGLGIVTFSTLYIFMPRRTVSVTT
                     HGMIKDYTLSSVEYRTRRTIREIIAWTASFELIAAFVIWPTLAKNGYSFYDALFHAIS
                     AFCNAGFSTLRSGMESFRDDVLLNVVTMLLIVSGGIGFIVLQDIMRFVRKEKRHLSYH
                     SSVVLRTTAVLIVLGAIVFYVLEQNHAYKEMSQGSKVMAALFQSVTPRTCGFDTVSQA
                     NLSSSSQLVTIILMFIGASPGSTGGGIKTVTLYILLLVALRMREGGDTIVDRYRTVTH
                     NAVLKAITALVRGATIVLVATTILLVSERAAGHTVGLETAIFECVSAFGTVGLSIGIT
                     PYLTAISKLVLIGAMFMGRAGLFILAIPPSRYSPEPFAKYPKADLLL"
     gene            4415..5182
                     /locus_tag="PK105_04330"
     CDS             4415..5182
                     /locus_tag="PK105_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOU60968.1"
                     /translation="MKQFAFIGLGPFAMSMLERISEISGQIIAADESPERIEHVKEMV
                     SVAFTMNLLDEEAFERAFHEPVDVAIVDIESNGPELLLVTFRLKKLGVPEIIVKSNSE
                     EYEEILRLVGATRVVNSDREAALRVTPLVLSSSLTNFMPIGGDLVLAEVIAPDFVVGK
                     SVIEGDLRNTRHINVVAVKYASEMDSSGSQSAIFRNLDTKYRFKDGDVLLVTGKENDV
                     FEFSGIQKTIEHEKERAKTISFLKGMFSRKKKDVPKK"
     gene            5348..6526
                     /locus_tag="PK105_04335"
     CDS             5348..6526
                     /locus_tag="PK105_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU60969.1"
                     /translation="MDIFQKCFDFDLDKQAMEKGLYPYFRPLDGLEGTEAVFNGRKLI
                     MIGSNNYLGLTMHPKVKEAARKGLEEFGPSCTGSRFLNGTLKLHNELEERLATFVGKE
                     AALVFSTGMQTNLGAISSLVDRNDVVIIDKDDHASIVDGCRLSMGEMKRYLHADLDHL
                     ERILQKVPSESGTMVVVDGVFSMGGDIVDLPGLVRLCQKYGARLYVDDAHSIGVLGDG
                     HGTAWHFGLTDKVDIIMGTFSKSFASLGGFVAGDRDVINYIKHTARSFMFSASLPAPN
                     TMAALAALDIMETEPEHVKHLWDNANFMMKGFRELGFNIGKTQTPIIPVIIGEDETCF
                     RFWKELFDEGLYTNPVISPAVPEGMALLRTSYMATHTREQLQRALDIFEKAGRKFGII
                     "
     gene            complement(6752..7459)
                     /gene="radC"
                     /locus_tag="PK105_04340"
     CDS             complement(6752..7459)
                     /gene="radC"
                     /locus_tag="PK105_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOU60970.1"
                     /translation="MKEWQAYEKMGQKFESNSSSLDIRERMLKLGSGSISNLELIVAI
                     LGSGVPGKPVRKLAKEVLNQITKSSDSCDLDQLMLISGMGEAKSCAVAAALELGRRIF
                     STRGTRIAMPKDAYPLLIHFADIRQEHFIVISLNGGHEVNAIREITKGLINKTVVHPR
                     EVFADPITDRACAVVVAHNHPSGNLEPSDEDVDITKRLRQSGDILGIPVLDHLIFSES
                     GYFSFMEHGLIVPTKGN"
     gene            7815..8390
                     /locus_tag="PK105_04345"
     CDS             7815..8390
                     /locus_tag="PK105_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOU60971.1"
                     /translation="MKSIKGTKTEANLLTGFAGESQARNRYTYFASKAKEEGYIQIQL
                     IFEETANQEKEHAKRLFKFLEGGTAKVSAEFPAGVIGTTRENLYEAAGGENYEWQEMY
                     PTFAKVARDEGFEAIASVFDSIAVAEKQHARRYEALMTNIDEGRVFKREEPVIWRCIN
                     CGYVYEGTEPPKACPACAHPQDYFELLAENW"
     gene            8409..8783
                     /locus_tag="PK105_04350"
     CDS             8409..8783
                     /locus_tag="PK105_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOU60972.1"
                     /translation="MTERKQVYKCMKCGNIVEVLHAGQGQLVCCGEPMRLMVENTSDG
                     AKEKHVPVLEKTADGWWVMVGSILHPMEEKHYIEWIELISDEHSYRVYLAPGQEPKAF
                     FPVKAEKVQAREYCNLHGLWKT"
     gene            8811..8972
                     /locus_tag="PK105_04355"
     CDS             8811..8972
                     /locus_tag="PK105_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOU60973.1"
                     /translation="MNKYVCDVCGYIYDPAEGDPEGKIPAGTPFEQLPDDWVCPMCGA
                     SKDSFSPMD"
     gene            9111..9401
                     /locus_tag="PK105_04360"
     CDS             9111..9401
                     /locus_tag="PK105_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60974.1"
                     /translation="MISRDDFVFCVGYDGDTAIIDGKAKKEFCGLSTMELAEKGLYRA
                     AFASALYSQNPEEMKVFIDFFNKKAGTAYTEAGQLSRLFGVYLETISKVKAL"
     gene            9445..10938
                     /gene="radA"
                     /locus_tag="PK105_04365"
     CDS             9445..10938
                     /gene="radA"
                     /locus_tag="PK105_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOU60975.1"
                     /translation="MGASIEPTLRLIYDYGNMKKPSTIYRCSACGHEEPKWLGRCPEC
                     GQWNTMVEAKTIGRFEKDKTAFSVPLESVDPALGARVSSGISELDRVLGGGFMRGSAV
                     LLGGEPGIGKSTLLLQACSKLGAKGKALYISGEESAAQIRLRAERIKALSKQIEIFCG
                     NDMHACLSVMDSAHPLLTVVDSIQTVHSEEAGAVPGTPNQIKFCTQEFVEWAKSHDSI
                     VVLVAHVTKDGMIAGPKAAEHLVDAVISFEQAENALRVLRTSKNRFGSSDELGFFLMS
                     EGGLSELQDPSGIFMIRREGQLPSGIALAIVYEGSRILLAEVQALTIPSKSGIMRVYS
                     DRIDPLRVSRIAAVLEKQTRLDFSSQEIYVNVAGGLRLTEPAVDLPLACALYSARSGQ
                     PLPEGSALAGELSLAGEIRPVRSMDRRTRAAHQLGFARIIGPKALPAGAEVPAKRGAA
                     KAEVVGAEAAKSGTVRVEAAALSGQALPWLGASSLRESLRILWGQGT"
     gene            10940..13156
                     /locus_tag="PK105_04370"
     CDS             10940..13156
                     /locus_tag="PK105_04370"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOU60976.1"
                     /translation="MQFINNGQRIAGLVVPLLSLKKNENAACGVFSDLVSLGKIAKKW
                     GLSLTQLLPLNDTGNGPSPYAAISAFALHPIYISLSDTIDTMALLGLPFKDEVAKELV
                     YAEKALDRRFGNGGRIQRVQFEAVLQRKIDALRKAWEASRQICSPLAEAFASRESWAK
                     PYACFVALREKFGLAPWWEWPEWQNVLPADIDKLWSNQELAEEERFRLWLQVLAQDQL
                     RHAAQTAHELGIDIMGDIPILLAKDSADVWFQREIFILDKQAGAPPDMYSPRGQNWGF
                     PLYDWNALRARNYDFWRQRLQAAENFYSGYRIDHVLGFFRIWAISIHESDAYLGAFEP
                     SGYLEPAELNSMGFSDERLTWLSKPHIPEWKIQKAEKRCLDAGMPLLAESEASGIENK
                     QKGKREENREDNREDERADIRALFGKRLEELRKSCFVRIKNEPLFLFKPEIRGTADLN
                     ALFGPLWSEFPEAAPIIDEYAGIMRDWWIDRTLYRYEPDRFVFQWSYRETTSWKSLSL
                     QEQQALEAKAKALSEESLNIWEKRGRKLLSMLVSASEMQAFAEDLGAVPPCVPKVLRE
                     LKIPGLRVLRWEREWDKPNQPYIPFDRYEALSISCTSVHDSTSLRQWWQEEADRSQIW
                     AICQVLAEHDELLKSLLQRCGDTAPEQLGSKEAAVLVRALALSGSIVAAYPIQDILAC
                     HTEWRLPNAQDERINVPGTTLPTNWMYRMPIDLQALAKDEDFAGFISKVMARGGQA"
     gene            13153..14103
                     /locus_tag="PK105_04375"
     CDS             13153..14103
                     /locus_tag="PK105_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOU60977.1"
                     /translation="MSNSAQRMQQIAHTTIKRTIDTSLFLIKIMVPVSLLVALLGWSG
                     LLEKISVVLHPIMRLLGLPGEAALVYISGALLNNYSAIAVMSSMNLSLHHAIILAVMC
                     LISHNLIVETTVMKSIGSSGTKMVILRVGVAFLAGFILNLILPSSYSEVQLFRTTAVA
                     HSPFWPMIGAWAISTLKLIGRIILYLLILMLAETLLEQFNLMERLSRWLGWLMRIFGL
                     KPSMSFLWIVINIVGYAYGAGIIKAARDEGRMTAKEGDLFNHHAAISHSILEDTVLYG
                     AISLPVFWLILPRILLSIIVVWLERLRRELYRRSLKAGVA"
     gene            14210..14800
                     /locus_tag="PK105_04380"
     CDS             14210..14800
                     /locus_tag="PK105_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOU60978.1"
                     /translation="MKFAEPQTTETESKKSEESLNERFLKTRTVLLVGEIDKDLSEKV
                     VRQLLLLESISKEPITLLIDSPGGDVYAGFSIFDVIRFIKAPVRIVGIGLVASAAALI
                     LIAVSKDHRFGLPNSSYLIHQPLSGMNGVATDIEIHAKELERTRLHINELLAEATGQA
                     LEKIIKDTDRDFWMNAQEAMQYGLIGKVIVSRQELD"
     gene            14910..>15779
                     /locus_tag="PK105_04385"
     CDS             14910..>15779
                     /locus_tag="PK105_04385"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU60979.1"
                     /translation="MRPAFLFLSVLAMLAAGCQCSLPALRTDGEINIVSYNVHNLFDA
                     EESGKEYPEFRPSKGKWTKELYAKRLGNVVEAVRSLSNTKDGEGSPGSDPDILCFQEL
                     ENEKVLFDLAKHLGHGKLRYWAMSGPEESIIHTGILSRFPIVAVHSHSVMDAWGLGML
                     RDVLEVEFECGSMGRMVIFACHWKSKLEGEAETEPARRAAAALVANRIRLLREERPFV
                     PIIVCGDFNESPDEYLRTNRCYPTAIMYSGVGVPPMAIEGEPIWACSSLEEIGDESSE
                     LAESHSQVGIINGE"
BASE COUNT         4160 a         3498 c         3972 g         4149 t
ORIGIN      
        1 tgtctaatct atccttaggc cctccaaaac ttccttgtaa aaagcgcaag caccgtatac
       61 acctcaagcc ttccgacaag catatccagc gaaagccaga gctttgccca ccaggggaaa
      121 agcgcatagt taaaggctgg cccgaccttt cccagtccag ggccaatgtt gccaagatta
      181 gcgatgactg ctgttatatt cgttagtaaa tctacgccac taaaggctat tacaagtacc
      241 gaaagaaaga aaaaagcaaa gtagaggaaa acgagaccgg atatatcata gatgacattt
      301 ttcctcagcg ccctgtttcc caaaaaaata gtgtatacac ctcgtggatt tgtaacatag
      361 cgcatctcgt ttactgccat tttgaagagc actacaaggc gaatcacttt gaccccacct
      421 cctgtggagc cggaacatcc gccgataaac atcaaaaaga aaagtacagt ctgagatagc
      481 tgtggccatt gcccaaaatc cgtggaagca aaacctgtgg tcgtaagaat cgaggatact
      541 tgaaaagacg catggcgcaa cgaatctccg atatttacaa atactcccga tacgaatagg
      601 tcgactgcaa taattactga tgatatagca aaaattccta agtaaaccct aaattcggtg
      661 tcaagtaaga ttgtgctcat tcgtcctttt aggaaatgat agtgcagggt aaagtttgtg
      721 cctgcaagaa acatgaaaat cgtaataacc cactggataa atccgccttt gtaataggca
      781 atgcttgcat ttttggtacc gaagcctcct gtggccatgg tggcaaaact atgacagata
      841 gcgtcgaaaa gtggcatgcc acccaagaca agaaggataa tttcgcagac tgtaaatcca
      901 cagtaaatca accatagggt ttttgcggtc ttggaaatac gtggggtaat cctatccacc
      961 tcaggactgg gggactccgc ctccataatt cggattgcgc taaagcccag actaggtaga
     1021 atagcaacca aaagcacaac aattcccata ccaccgagcc agtgcgtggt tgctcgccag
     1081 aggagcagag gtcgaggtat tatctcaata tctgtcaata tgcttgctcc tgtcgtggta
     1141 aagcccgata ctgtctcaaa gaatgcgtct atataggaag gaatataccc ggagaaggta
     1201 aaaggaatgg cgcctaaagc cgaagaaaac acccagctaa aggccacaaa aaggaaacta
     1261 tctttgtttg taagaaaggg tctttcatca tgccttgtca ttaaatacat tactataaaa
     1321 aatgctaggc tgatgatggt aggtatgaag agcgaaaagc tcacccgcca ggataggtct
     1381 tcgacaatag agtaaattcc gcagatcaag ctaaaaatcg agacaatcaa aagaataatg
     1441 gaaagcattc tgagaatcga tttgaaatgc atatcatgcc ccaaaaatgc tctcaagctt
     1501 gcctatgtgt tcttttttga taattagtcc cacaagatcg ttttcgaata tctttgtaga
     1561 gcccgtggga atgcaggttt ttcctctatg gatgacaaaa caaatcagca atccctttgg
     1621 aagattcatg ttgcgtatct ccacttcgag ctgctcgaaa gagtccgtaa tctgaatctc
     1681 gatgagctca tattcattat gtggaaagct atagagtctc cttattttgg ccttccttac
     1741 cttgtcgagt attgcgcccg acataacgat tttttcattc acgacgatgt tgatatcgag
     1801 caaatagaga agcggagtgt agagatcgtt tgtaatgagc gctaggcttc gctttgctcc
     1861 tgcttcttgg gctagaagag cgctcagaat atttatgctc tgttcagatg caaggcagag
     1921 aacaatgtcc gcgcttccga tgttctcctc cctgatcaaa tgctcatcgc tcaactgatg
     1981 atttattgct tctatatcgg ggaaagtagc cgagagaatc tttaagctct ctcgatctcc
     2041 gtatattacc ttgatttttg gatttccaag caatgagagg gctcccctat cgtacccctt
     2101 atcgcgctgt gaaccggaaa aagcagaaat ctgcctcatt ccgagctcct ttagcaagag
     2161 tgtcgtaaga gtgcctgtac caaggattac gatagagctg aatttcctga gagtgtcttt
     2221 gcatggtcca aaaagtctat cgaggctaga gggctcgcct aggatatagg ccgcatcttc
     2281 ttcttcgaaa acgaaatcac cgcccggaac gattagttct tcttcgcgca gcactgcggg
     2341 taccataaaa tccgccccaa tgctagtgcg gatattggcc agcgattggt gtacaaacct
     2401 tggctctttc ttacatagaa tgcgtcggag ctgaattcct gcttctttta catcgataat
     2461 ctcgggcgac atgccacgga aaatggtgcc cgcaatactc tcggcagttt cggcctcggg
     2521 gttgataata taatcgacac ctagaatctt ctttcctgca ttcttaaagg atgcaaaata
     2581 agggttgcgg gctcgggcta tggttcttac gtctttgaag ttctcggcaa ctaagccaca
     2641 ggagacgata ttggcttcat cgctgccggt acaagcgata aaccagtctg cgtcttcgac
     2701 accagcagcc ttaagagtct cgatgttttc gccatcgcct tcttggacga tgcaatccag
     2761 cttgtccgcg atcgattttg ccgcattcag atttttctct attataacga catctttgtt
     2821 ttccgctatg agttcgctgg caataagaga accgagcaaa cctgcaccaa gaatagctat
     2881 tttcatcgat gaaaagtata tgcgaccgag tgctattttg acaatgacag ggctcggatg
     2941 aagcgcttgt tgtatgccca acaattgggc ggcgccaaag atcgaaaaag ccctataaat
     3001 aggcgcatca acatcgacat attgagcctc attcgcattt tgcatactat aatgcgacag
     3061 gagcctcagt tgagaaaccg tttgggcaaa gaaaagcaga ttcctctatt atatttttta
     3121 ggcactattt tggccgggag cttccttttg gtgttgccaa tatcgagcga ggaaggcaac
     3181 ctctcatacc ttgatgcact tttcgaggcg accagcgcgg tctgcgttac cggtcttgct
     3241 gtcgttgata cgaccaggct ctctcgattt ggccaaacag tcatcctttt cttgattcag
     3301 cttggaggct tgggtatagt taccttttcc accttataca tttttatgcc aaggcgcaca
     3361 gtgtcggtga caactcatgg tatgataaag gattatactc tatcgtcggt tgaatatcgc
     3421 acgcgcagaa cgatccgaga gattatagca tggaccgcca gtttcgagct catcgctgct
     3481 tttgtgatct ggccaacgct tgcaaaaaat ggttactcct tctatgacgc gcttttccat
     3541 gcaatctcag ccttctgtaa tgctggattc tcgactttgc gatcgggcat ggagagcttt
     3601 cgggatgatg ttcttttgaa tgtggtgacg atgctgctca tagtctctgg agggataggc
     3661 tttatcgtgc ttcaggatat tatgcggttt gtcaggaaag aaaagcgtca tctttcctat
     3721 cattcctcag ttgttttgcg cactaccgcg gtgctaattg tgcttggggc tattgttttc
     3781 tatgtgctcg agcagaatca cgcatataag gaaatgagcc agggttcgaa agtgatggca
     3841 gcgctttttc aatcggtaac tccaaggacc tgcggtttcg ataccgtgtc acaggcgaat
     3901 ctctcgagtt caagccaact tgttacaata atcctcatgt tcattggcgc ttcgcctggc
     3961 tctactggtg ggggcatcaa aactgtaacg ctatatattc ttcttcttgt cgcattgagg
     4021 atgagagaag ggggagatac gattgtggac cgatatagga cagtgactca caatgcggtg
     4081 ctcaaggcaa tcaccgcgct tgtgagaggc gcaaccattg tgttggtcgc caccacaatc
     4141 ctcctcgtct ctgagagagc agccgggcac acggtaggac ttgagactgc tattttcgaa
     4201 tgtgtttctg cctttggaac cgttggctta tccataggca taactccata ccttacggct
     4261 atttcgaagc ttgtccttat aggcgcgatg ttcatgggga gggccggatt gtttattctc
     4321 gcaattccac cttcgagata ttcaccagag ccttttgcaa aatatcctaa ggcagacctg
     4381 ctgttataat ttgttcttat atggaaggta taatatgaaa caatttgcgt ttattggtct
     4441 gggaccattt gctatgtcaa tgttggaacg gatttccgag atcagcggcc agattattgc
     4501 cgcagatgag agccctgagc gcatcgaaca tgttaaggag atggtctcgg tggcttttac
     4561 aatgaatcta ctagatgaag aagcattcga acgggcattt cacgagccag tggatgtggc
     4621 cattgtcgat atagagtcga atggtccgga gctcttgctg gtgactttca ggctcaagaa
     4681 attgggcgta cccgaaatca tagtaaaatc gaactcggag gaatatgaag aaattctgcg
     4741 gctggttggt gcaactcgcg tagtaaactc ggacagagaa gctgccctaa gagtaactcc
     4801 acttgtgctt tcttcttctc tcacaaactt tatgcctata gggggtgatc ttgtacttgc
     4861 agaagtcata gctcccgatt ttgttgtggg taagagcgtc atcgagggtg atctgcgcaa
     4921 tacgcgtcat atcaatgttg tcgccgtaaa atatgcttcg gagatggatt cctccgggtc
     4981 acaaagcgca atctttcgta atctcgatac aaaataccgc tttaaagatg gcgatgtgct
     5041 gcttgtaacg ggcaaagaga acgatgtttt cgagttttca ggtattcaaa agactatcga
     5101 acatgaaaaa gaaagagcca agacgataag ttttctcaaa ggcatgttca gccgcaagaa
     5161 gaaggatgtc ccaaagaagt agcctttggc ttgtgcgagc ctgcgcgctc gttgattcca
     5221 ttcgctcgaa ccattacaat agaaaagcgg ctcaggcatg atatacagcc tgtgcgctcc
     5281 ggcggcaaat agccgttccg gcaaactgcg ttgtggaaag gatccgcgcg cgcaatggag
     5341 gtcttttatg gacatttttc aaaagtgctt tgattttgat ctcgacaagc aggccatgga
     5401 aaaagggtta tatccctatt tccggccgct tgatgggctc gagggaaccg aggctgtctt
     5461 taatggtcgc aagctgatca tgatcggttc taacaattat cttggactca caatgcatcc
     5521 aaaagtcaag gaagccgcta gaaaaggtct ggaagaattt ggtccttcct gtacaggaag
     5581 ccggttccta aatggtacgc tcaaacttca caatgagctc gaagagcgcc ttgccacttt
     5641 tgttggaaaa gaagctgccc tggttttttc gaccggcatg cagactaatc ttggagctat
     5701 ttcatcgttg gtagatcgca acgatgttgt cattattgat aaagatgacc atgcttctat
     5761 tgtggacggt tgtaggctca gcatgggtga aatgaagcgc tatttgcatg ccgatctgga
     5821 tcatctcgaa cgcattctgc aaaaggttcc cagcgaatca ggaacaatgg tcgttgtcga
     5881 tggtgttttc tccatgggag gagacattgt cgacttaccg ggcttggtaa gactctgcca
     5941 aaaatatgga gcgcggctct atgtggacga tgcacattcc attggcgttc tgggcgacgg
     6001 ccatggtacg gcctggcatt ttggtcttac cgataaagtc gatatcataa tgggcacctt
     6061 ttcaaagagc tttgcatcgt taggtggttt tgtggcaggt gatagagatg taattaatta
     6121 tataaaacat acagcccgct cttttatgtt ttctgcttct ctgccagcac ctaataccat
     6181 ggcagccctt gctgctctag atatcatgga gacagagccc gagcatgtga aacatctttg
     6241 ggataatgcc aatttcatga tgaaaggctt tcgagagctt ggttttaata tcggaaaaac
     6301 tcagacccca attatccccg taattatagg tgaggatgaa acatgcttcc gtttttggaa
     6361 ggagctcttc gatgaggggc tctataccaa tccagtgata agccctgccg taccagaagg
     6421 aatggctcta ttgcgcactt cctacatggc gactcacact agagagcaac tgcagcgcgc
     6481 gctcgatatt ttcgagaaag caggcaggaa gtttggtatc atctagtcaa taatatccga
     6541 agttagcttg cggcggtcaa aatggcattt caggcgcgga tagcaaaata ggcggcagca
     6601 gatggcaaaa tcgacagcat cggctggctt ttggactatc tgctcaggct atctgcgccc
     6661 ttttttattt tatatttcgt ttggcagagt cttatcggta cttttctgtc tcctttgagc
     6721 tctgcgattg gccagagctt gctagcgcgg actagtttcc cttggttgga actatgagcc
     6781 catgttccat aaaactgaaa tagccggatt cagagaagat aagatgatcc agcacgggta
     6841 tacccaggat atcacccgat tgccgaagcc ttttggttat gtccacatct tcgtccgaag
     6901 gctccagatt cccgcttgga tgattgtgcg caactacgac cgcgcaggct ctgtcggtta
     6961 taggatcagc aaagacctcc ctgggatgca caaccgtttt atttatgagt cctttggtta
     7021 tctcgcgaat ggcattaacc tcatgaccgc catttaacga tatgacgatg aaatgctctt
     7081 gtcttatatc cgcgaaatgg ataaggagtg gataggcatc cttgggcatt gctatgcgag
     7141 tgccgcgagt cgagaaaatc cgtctcccaa gctccaacgc ggcagctacc gcgcaactct
     7201 ttgcttctcc cattccagat atgagcataa gttgatcgag gtcgcatgaa tcgctgcttt
     7261 tggtaatctg gttcagtacc tctttggcaa gcttgcgaac gggtttgcct ggaactcccg
     7321 aacccagaat tgcaacgatc agctcgagat tagatatcga gccgctgcca agcttcaaca
     7381 ttctttccct tatatccagc gaagaggagt tagattcaaa tttttgtccc attttttcgt
     7441 atgcttgcca ttccttcatt gtgtctccta atgtctatga cgcatcaaga gtgattctcg
     7501 attcaatgtt tttcggctta gatatcatcg aaatagcatc caaatagcat ccaaaatgct
     7561 attctatcac tatattagct ccaaaaggct caagaatgcc caaagtagcc ctataaaagt
     7621 acaatgaact cataagaaag ccctaaacct aaaggaaccg caaggtcaca catgtagaga
     7681 tcagtacaaa taagaatccg ctgaaagtat catcaattgg ccgatcaggc atttgcgttt
     7741 cgagatcggc tggaatataa tcaaaagtga tacttgattt ttgggaaaat tcccaatttc
     7801 gagaggagga gattgtgaag agcataaagg gcaccaagac agaagcaaat cttcttactg
     7861 gattcgcggg agaatctcag gcgcgcaatc gctatactta tttcgcctcg aaggcaaagg
     7921 aagaaggtta tatccagata cagcttatct tcgaggaaac ggcaaatcaa gaaaaagagc
     7981 atgcaaagcg attattcaag tttctagaag gtggaaccgc aaaagtttcc gccgaatttc
     8041 cagccggtgt aatcggcaca acgcgagaga atctctatga ggcagcgggc ggagagaatt
     8101 acgaatggca ggagatgtat ccaacttttg ccaaagtggc aagagatgag ggttttgagg
     8161 caattgcgag cgtatttgat tccattgccg tggctgaaaa acagcatgcc aggagatatg
     8221 aagcgcttat gacgaacatc gatgaagggc gtgtcttcaa acgggaggag cctgtgattt
     8281 ggcgctgcat aaactgcggt tatgtctacg agggaaccga accgcctaaa gcatgtcctg
     8341 catgtgctca tcctcaagat tatttcgagc tattggccga aaattggtaa tttaggaggc
     8401 tatggtatat gacagagaga aagcaggtct acaagtgcat gaagtgcggg aatatcgtag
     8461 aagtgcttca tgcaggccag gggcagcttg tatgctgcgg agaacccatg agactcatgg
     8521 tagagaacac gagcgatggt gcaaaagaaa agcatgtgcc tgtacttgaa aaaactgccg
     8581 atggttggtg ggtaatggtt ggttcgatct tacatccgat ggaagaaaag cactacatag
     8641 aatggattga gcttatctct gatgagcatt cctatcgggt ctaccttgcg cccgggcaag
     8701 aacccaaagc ctttttcccc gtaaaggcgg aaaaggtgca agcaagagaa tattgcaatc
     8761 ttcacggatt gtggaagaca tgagagatcc taattcttaa ggagcgcatt atgaataagt
     8821 atgtatgcga tgtctgtggt tacatttatg atcctgcaga aggagatcct gaggggaaga
     8881 tacctgctgg gactccgttc gaacagctgc cggacgactg ggtctgccct atgtgcggtg
     8941 caagcaagga ttcattttcg ccaatggatt gaaaaagagc tctgcccgat atttggcggg
     9001 tattctttcc agaatggcac aataagaaag agggagtctg cgctccctct tatttaattt
     9061 tatatatcgc attattctaa ggcattattc tctgagagct tggagtgtct atgatttccc
     9121 gcgatgattt tgtgttctgt gtgggttatg atggcgatac cgcgattata gatggcaagg
     9181 ccaagaagga attttgtggg ctttccacca tggagcttgc tgaaaaaggt ttgtaccgtg
     9241 cggcttttgc gtccgcgctc tattctcaaa atcccgaaga gatgaaagtc tttatcgatt
     9301 ttttcaacaa gaaagcaggc acagcctata cagaggccgg gcagctttcc aggctttttg
     9361 gtgtgtatct ggaaacaatt tcaaaggtaa aggcattata gctcgtgccc ctctcgatga
     9421 gtcgaaaaca gatcatgggg gtcattgggc gcgtctattg aacctacgct tcgcctcata
     9481 tacgattatg gcaatatgaa aaagccttcg accatatatc gctgttcagc ctgtggccat
     9541 gaagaaccaa aatggcttgg acgttgtccc gagtgcggcc aatggaacac catggttgaa
     9601 gccaaaacca tagggcgctt tgaaaaggat aagactgcat tttccgtacc gctggaatcg
     9661 gtggatccag ctctgggggc gcgcgtatcg tctggaattt cggaactgga tcgagtgctt
     9721 ggtggtggct ttatgcgagg ctcggcagtg ctcttgggtg gtgagcccgg gatagggaaa
     9781 agcacccttc ttctgcaagc atgctcgaag ttgggggcaa aaggaaaagc gctatatatt
     9841 tccggagaag aatctgcagc ccagatacgc cttagagccg aacgcatcaa ggctctttcg
     9901 aagcagatag agatattttg tggcaacgat atgcatgctt gtctctctgt gatggattca
     9961 gcacatccgc ttcttactgt tgtggattcg atccagacag tgcattcaga ggaggcgggg
    10021 gctgtgccag gaacgccgaa ccaaatcaaa ttttgcaccc aggaattcgt cgaatgggca
    10081 aaaagccacg attctattgt cgtccttgta gcgcatgtga caaaggatgg aatgatagcc
    10141 ggacccaagg cagcagaaca tctcgtggac gcggtaataa gcttcgagca agcggagaat
    10201 gccctacgag tgctacgaac cagcaagaat cgctttgggt cttcggatga gctaggattt
    10261 tttctgatga gtgaaggggg gctttcggag ctgcaggatc catctggaat atttatgatc
    10321 aggcgcgagg gacagttgcc ttcgggcatt gccctggcaa ttgtatatga aggatcgcgg
    10381 attctattag cggaagtaca ggctttaacc atcccttcga aatcgggcat catgagagtg
    10441 tactccgata gaatcgatcc tctgcgtgtc tctcggattg cggctgtgct cgagaagcag
    10501 acaaggctag atttttccag ccaagaaatc tatgtgaatg tcgcgggagg tttgcggcta
    10561 acagagcctg ccgtcgattt gccactggca tgtgctcttt attccgcacg atcgggccag
    10621 ccattgccgg agggttccgc tcttgcaggt gagctttcgc tcgcagggga aatcaggccg
    10681 gtgcgttcta tggatcgtag aacaagggcg gctcatcagc ttggctttgc gcgtattatt
    10741 ggaccaaaag ccttacccgc tggcgcggag gtcccggcaa agagaggtgc agcaaaagcc
    10801 gaggtcgttg gagcagaagc ggccaagtct ggaacagtca gggtggaagc ggccgctctt
    10861 tcgggacagg cattgccctg gctgggggct tcttctcttc gagaatcgct gcgaattcta
    10921 tggggtcagg ggacctaaaa tgcagtttat caataatggg caacgaattg caggacttgt
    10981 cgtgccgctt ttatcgctta agaagaatga gaatgctgca tgcggagtgt tctcagatct
    11041 ggtgagtctt gggaaaatag caaagaaatg gggactttct ttgactcagc ttctgccgct
    11101 caacgacacg ggcaatggac catcacccta tgcggccata agtgcctttg cactacaccc
    11161 aatctatatt tctctctccg atacgatcga tactatggcc ttactcggtc tgccttttaa
    11221 ggacgaagta gcgaaggaac tggtgtacgc cgagaaggct ctagatcgta gatttggaaa
    11281 cggcgggcga atccaacgag ttcaattcga agcggtgcta cagcgcaaaa tagatgcgct
    11341 tcgcaaggca tgggaagcaa gccggcaaat ctgtagtcct cttgcggaag cctttgcaag
    11401 ccgagagtcg tgggcaaaac cctatgcctg ttttgtcgcc ttgagagaaa aattcggtct
    11461 agcaccctgg tgggagtggc cagagtggca aaatgtcttg ccggccgata tagacaagct
    11521 ctggtctaac caagaattag ctgaagagga gcgatttcgg ctttggcttc aagtcctggc
    11581 tcaggatcag ctaaggcatg cagctcaaac agcacatgag cttggcatcg atatcatggg
    11641 cgatatacct attctccttg ccaaggattc agctgatgta tggtttcagc gagagatttt
    11701 catcctcgat aaacaagccg gagcgcctcc ggacatgtat tcgcccaggg gacaaaactg
    11761 gggctttcca ctgtatgatt ggaatgcgtt gcgtgcgcgg aattatgatt tctggcgcca
    11821 acgcctgcag gctgctgaaa atttctacag cgggtatcgc atcgatcatg tgctgggatt
    11881 tttccgtatc tgggctataa gcatccatga gagcgatgct tatcttggcg cctttgagcc
    11941 ttctggctat ttggaacctg cagagctcaa ttcgatgggt ttttcagacg agcgccttac
    12001 atggctatca aagcctcata ttcccgaatg gaaaatacaa aaggctgaga aaagatgtct
    12061 cgatgcagga atgcccctgc ttgcagaaag cgaggcttca ggcatagaga ataaacaaaa
    12121 agggaagcga gaagaaaatc gagaagataa tcgggaagat gagcgagccg atataagggc
    12181 gctatttggc aagcgcctcg aagaactcag gaaatcctgt tttgtgcgca taaagaatga
    12241 gccactcttt ctattcaaac ctgaaatccg aggcaccgca gacctaaatg cactctttgg
    12301 gcctctgtgg agcgaattcc ccgaggctgc accaattatc gatgaatatg ccgggataat
    12361 gcgagattgg tggatagaca gaacactata tcgatatgag ccggatcgct ttgtctttca
    12421 gtggagttat agagaaacga caagctggaa aagcctttcg ctccaagaac aacaggcctt
    12481 agaggcgaag gcaaaggcac tcagtgagga atcgttgaac atatgggaga aacgaggccg
    12541 caaacttctt tcgatgcttg tatcggctag cgaaatgcaa gcctttgcgg aagacctcgg
    12601 cgctgttcca ccctgtgtcc ccaaggtatt gcgtgaactc aaaatcccag ggctgcgagt
    12661 attgcgctgg gagcgagaat gggacaagcc aaatcagcca tatataccat ttgatagata
    12721 tgaagcgctc tctatctctt gtacatcggt gcacgactcc acaagcttgc gacaatggtg
    12781 gcaggaagaa gccgatagat cgcagatatg ggcaatatgt caggttctag cggagcacga
    12841 cgagcttttg aaatccttgc ttcaacgctg cggagatacg gctcctgaac agctcggctc
    12901 taaagaagct gccgttctgg tgcgcgcact ggcactatca ggctccattg ttgccgccta
    12961 tccaattcaa gatattctcg catgccacac ggagtggcga ttacccaatg ctcaagatga
    13021 gaggataaat gtgcccggta ctaccctccc aacaaactgg atgtacagaa tgcctatcga
    13081 tctacaggcg cttgccaagg atgaagactt tgcaggcttt atttctaagg ttatggctcg
    13141 aggagggcaa gcgtgagcaa ctcggcacaa agaatgcagc agatcgcgca cactacgatc
    13201 aagagaacaa tcgatacatc gctgtttttg ataaaaataa tggttccggt tagcctattg
    13261 gttgcacttc ttggctggag tggattattg gaaaaaatct cagtagtctt gcatccgatt
    13321 atgcgacttt tgggtttgcc cggagaggct gcacttgtct atatctccgg agcccttctc
    13381 aacaattatt ctgctatagc cgtcatgagc tcgatgaacc tcagtctcca ccatgctatc
    13441 atcctggctg tcatgtgcct catttctcac aatctgatcg tagagaccac ggttatgaaa
    13501 tcaattggat cctcaggaac caagatggtg atcttgcgtg taggtgttgc atttctggct
    13561 ggctttatcc ttaatctcat acttccttcg tcctattcgg aagtgcagct tttccgtacg
    13621 actgccgtag cccatagtcc cttttggcca atgatcggcg cttgggctat atccactttg
    13681 aaactcattg gacgaatcat tctatacctt ttaatattga tgcttgcaga gacgcttttg
    13741 gaacaattca atctaatgga aaggctctcc cgttggcttg gatggctaat gcgcatattt
    13801 ggactaaaac cttcgatgag ttttctatgg atagtgatca acatcgtagg ctatgcctat
    13861 ggagcgggaa tcatcaaggc cgcgcgcgat gaggggcgca tgaccgcaaa ggaaggggat
    13921 ctcttcaatc atcatgcggc gatttcgcac tcgatccttg aagacacggt gctctacggg
    13981 gctatttcct tgccagtttt ttggcttatc ttgccgcgca ttttgctttc gataattgtc
    14041 gtatggctgg agcgcttgcg gcgagaactg tatcgacgtt ctctcaaggc aggagttgca
    14101 tgagtttttt aaattgtcag tatcgttgcc gttttatctc gaaatcgtta ctataaaatc
    14161 gagtatagat aaagagtctc ttacattatt gcaaggagtc ttataagaaa tgaaatttgc
    14221 agagccacag accactgaaa cggaatccaa gaaatcagaa gagtctttga atgaacgttt
    14281 tctcaagacc cgcaccgtgc ttctggtagg tgagatcgac aaggatcttt cggaaaaagt
    14341 ggtgcgtcag cttctattgc ttgaaagcat ttcgaaagaa ccgattaccc tgctcatcga
    14401 ttctcctggc ggagatgtat atgccggatt ttccattttc gatgtgattc gcttcataaa
    14461 ggcgccggtc aggattgttg gtatagggct tgtcgcttct gcggcagcgc tcatactgat
    14521 agcggtctct aaggatcacc ggtttggttt accaaattct tcatatttga ttcatcagcc
    14581 attatccgga atgaatggtg tcgcgacaga tatagagatt catgctaagg aactcgaaag
    14641 gacgcgtctg catatcaatg agctgcttgc cgaggcaaca gggcaagcgc tggaaaagat
    14701 tataaaagat accgatagag atttttggat gaatgcccaa gaagcgatgc aatatgggct
    14761 cattggaaaa gtgatagttt ccagacaaga gctcgactga agagttgggg catgtcttat
    14821 ccgggaatta ttgctgtttc ttgattttgg cgcgtgaatt ctgaagcgct tataatgccg
    14881 gatagcgtct ttcttattaa ggaggaaaga tgcgtccggc atttcttttt ctttctgtgc
    14941 tcgcaatgtt ggcggcaggt tgccagtgtt ctctgccagc cttgcggaca gatggtgaga
    15001 tcaatatcgt gtcatacaat gtgcacaacc ttttcgatgc agaagagagc ggaaaggaat
    15061 accctgagtt taggccttcc aaaggaaagt ggactaaaga gctctatgcc aagaggctcg
    15121 gaaatgtcgt agaggccgtt cggtcgcttt ccaacactaa agatggcgaa ggttctcctg
    15181 gttcggatcc tgatattcta tgtttccagg aactcgagaa cgaaaaggtt ctcttcgatc
    15241 tcgcaaagca ccttggtcat ggaaagctcc gctattgggc aatgagtggg cctgaagaga
    15301 gcataatcca taccggcatt ttgtcccgtt tccctatcgt ggccgttcat tcgcattcgg
    15361 ttatggacgc gtggggtctt ggaatgttgc gggatgtcct ggaggtggaa ttcgaatgcg
    15421 gtagtatggg cagaatggta atcttcgcct gccattggaa gtccaagctg gaaggagaag
    15481 cggagacgga accagctcga cgggctgcag cggcattagt cgcgaataga atacgattgc
    15541 ttcgagaaga gcgacctttt gttcccataa tcgtctgtgg cgatttcaat gaatcaccgg
    15601 atgaatacct ccgcacaaac agatgctatc ctacggcgat catgtattca ggtgtgggag
    15661 ttccacccat ggctatcgaa ggagagccaa tctgggcttg cagtagtcta gaggaaatag
    15721 gagacgaaag ctcagaactg gccgaaagcc atagccaagt tggcataata aatggcgaa
//
LOCUS       DAOOWY010000037        15765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3743_length_15765_cov_17.209293, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000037 DAOOWY010000000
VERSION     DAOOWY010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15765
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3743_length_15765_cov_17.209293"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            767..1069
                     /locus_tag="PK105_04390"
     CDS             767..1069
                     /locus_tag="PK105_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60980.1"
                     /translation="MPISDVSVKGSYIQVFDGAGKKVSEMFASGKEVVGTASDFFVVV
                     SGSYIQTYDEKCKKIAEMLSSGKEPRGASGNSFTVKSGSYIQTYDKNCKKISERFA"
     gene            1224..2237
                     /locus_tag="PK105_04395"
     CDS             1224..2237
                     /locus_tag="PK105_04395"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOU60981.1"
                     /translation="MQVWVFNSPDEKATDFIYSSLLKGFSRFGWSYIDTADLNILERK
                     RWEELNDDETECYRKTSFLLNIEIGDWIVHVNIPEWGKCTAAQVISTYEFDKEHNSIG
                     IDYTNFGDYRHLIKIDVNTLITFDRNNHNVLPYVSRRLKLQGHYWTIKKTDDFLQSIN
                     NLKSNAVNLEGNITHGLYHLRNDIRKSLKDISRMIQENHPGKQLEEFIALIFERVPNV
                     VDVKRNGSGWGTDYGADLIITYNTGIPILDFQTSEIIVVQVKSFTGLHDDINTIEQIA
                     TAIDKYNAKSGIIFTTAVVTDSFIAKVNDKSKSIGKAIGIISGDDVASFVLKYGSDLL
                     FDI"
     gene            2585..3688
                     /locus_tag="PK105_04400"
                     /pseudo
     CDS             2585..3688
                     /locus_tag="PK105_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739851.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
     gene            3829..3981
                     /locus_tag="PK105_04405"
     CDS             3829..3981
                     /locus_tag="PK105_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60982.1"
                     /translation="MGLSMQERHRVIAETAARYRAARKKEKCHILDELTALTGYDRKY
                     ALHLLT"
     gene            4026..4268
                     /locus_tag="PK105_04410"
     CDS             4026..4268
                     /locus_tag="PK105_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60983.1"
                     /translation="MIVIGTVQHRKKRTGKKKYSQELYEALQCIWATFDCMCDKRLAV
                     FIRENIAFLARHEEYAITDTLRAQLTTISPRHDYCQ"
     gene            4749..4889
                     /locus_tag="PK105_04415"
     CDS             4749..4889
                     /locus_tag="PK105_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60984.1"
                     /translation="MEDEETKPILLSPIEVRELEGMALAMESIAKGIRDILARGQEGE
                     KD"
     gene            5140..6039
                     /locus_tag="PK105_04420"
     CDS             5140..6039
                     /locus_tag="PK105_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOU60985.1"
                     /translation="MKKYLFVFALLLCVGSLFGQDLGAEVNARDKNGKTILMYAAMYS
                     QNPESITAWLNAGSEVNARDKDGSTALMLAAGFNQNPEVITTLIKAGAKLDDRDTAGR
                     TTLMYAAELNSNPEVITTLLKAGAKLDDRDTNGKTALMLAACYNPNPEVITTLINAGA
                     EVNAREEILGLMSLMWAAANNPNPEVITILINAGAEVNARNKDGITALMLAAMNNPNP
                     EVITTLIKAGAKLDDRDTNGMTALMVAAHFNPNPEVITILINAGADGKLKSSKGKTAF
                     DYAKDNPKIKDTAAYWALNDARF"
     gene            6208..6480
                     /locus_tag="PK105_04425"
     CDS             6208..6480
                     /locus_tag="PK105_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60986.1"
                     /translation="MNKKRLTDDQGLKMELAGVMCRLSSSVSILAGNLVVSIEVQQGE
                     SGTMAVKDEVDHTPPRALTNASAFFVRDEHSQRQKIGGKGSFLQVL"
     gene            complement(7246..9567)
                     /locus_tag="PK105_04430"
     CDS             complement(7246..9567)
                     /locus_tag="PK105_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU60987.1"
                     /translation="MKMNWMHLPLWRLARLGFVSLLICFAVTWPAVADGSEVQTPRPK
                     IAVVLSGGSAFGIAHAGVLEKIEEAGIPIDMILGTSMGSIVGGLYAAGYSPQDMQELI
                     SDIDWNSLFMDSKALPQNMFQRAVESMYALRIGFDSKGPNIGAGLLEGQNILAFFTAR
                     TIHMATKRNFDEFPVPYRAIAANILNGDTVVFDQGSLAEAMRSSMSIPVVFMPYEHNG
                     QLLVDGGIVDNLPVNIAKEMGADIVIAVVSRGKAPDSIDDLNTSVEIASQTGNLFIMQ
                     NMRPNLEAADLVITPNLEEFTTASFARAKEIIARGEEAGDIAMPQLRELATKIGEQRA
                     LVSPSEQANRKAFAPPPMFSSLRVEGGSESDRDKIAGIFAPLIGKAYSREALENALHA
                     AFIAGDYSLVKFDLESLKDGEQSESLRGVVSIVPQKPTQDELFASLGFESAISNDISS
                     NLVASLAFLAKELTGPGSALFSSVSFVNKTSASLEYFQPFGSFFILPWARFRFEYDMF
                     VSESLPVAIASKFRTYGAGAWGGLALGNVGDIMAGYSFENVLTGDDWTNLAAQNAGAL
                     RAALRLDTRDRTVFPRNGLSLNAFGRWFSPNFGGQFSFSQIEINASAAIPVGQTDALH
                     LSLFGGTDFAGLVDKAQPAKISYYSTLNQPLMFYGLIYTQDDCSGNTVLAGSLEFRHR
                     IRVINELTGGDIYIFANGSAGAVAQYDDPSTYAMWPLKWSATLGASVSIAPHFGIFLG
                     ASVLGNIDDTAAIVPAFVIQVGSFNDLSIIDKR"
     gene            complement(9741..>10671)
                     /locus_tag="PK105_04435"
     CDS             complement(9741..>10671)
                     /locus_tag="PK105_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60988.1"
                     /translation="IKEEAMKRFLWLGLLVVAIFLVGCPINPPDTENTNITIDDIKGE
                     WDFSNVKINGKVYNPVHVFIEKGAHGTESQYHNRVDITIGDSSYYWSDGDFDDNVYKG
                     EYTYGVKDDQGNIHQYDEPQDIEITFSLIDGKLRMQVQADGLLGSFTIELFEGGENQE
                     NEGESPVLTPAEYSQVEGNWTFTNVWIPNQPEGVGFSFFTTCTLNIYTEHIGESSIET
                     MHFTGVDNHGYTCKYDGQFASSEGMIYQYSYNYDIKDAGGNLKAESDYAKTITVKFFL
                     TGDGKLFVEFDSDGQPDPGPLDGVMLTYGEKQD"
     assembly_gap    10672..10771
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10968..12044
                     /locus_tag="PK105_04440"
     CDS             10968..12044
                     /locus_tag="PK105_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOU60989.1"
                     /translation="MTTIVLSGGGGTRLWPLSRQAIPKQYCNLLGGLTLFERTIKRNI
                     KLSDNIIVVTNVAHFDIAKSQFRKVCPDKQVHFILEPCGRNTAPGIALACLELDPEEN
                     VLVVPSDHIILDEEAYQDAVNRALVAANLGYLVTFGLKPEYAETGFGYIEADGDKETA
                     GEGFLLVKAFKEKPDRATAEEYIAAGRYYWNSGIFSFLAGKYLYELGNTAPEILLASK
                     KAWEAARKSISQNTEDVIAIPEEAMLSIPAESIDYAVMEKSQNVAVVPCSLGWSDLGS
                     WDAIWKIRERSGLEALEGSSTISIESKRNLVVGSERIVALVDVEDLIVVDTPDALLIS
                     KRGSSQNVKRIVEQLKEKGKTHLL"
     gene            12174..12779
                     /locus_tag="PK105_04445"
     CDS             12174..12779
                     /locus_tag="PK105_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU60990.1"
                     /translation="MGRERLFADKKEIIDAAIELLSENGYESFSTRKLARRLGVSPMT
                     LYNYFSSRNEIIEAAIEAAYERVFQEFYNDLSSYLEKESFCPLCGFMEIGKKLLLFSR
                     EKPKIYALVFIMNIMPYREQPSVQRLFGITLSKVRARLTAEEISQDLQNHIYLFMLLV
                     LALVQNVYNGNSSYDIRAFEANLRIAYNQLLKPFEKYFSFA"
     gene            12899..13630
                     /locus_tag="PK105_04450"
     CDS             12899..13630
                     /locus_tag="PK105_04450"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOU60991.1"
                     /translation="MGLFQKKKHFLAFIPCIFGLVIWSLIPIAAGAQSEPGHSIQVYG
                     QASMYVEPDVANLLIGIEIQADSAAKAQEQLRYQASVVFDAISQASIQQPKMKTSSYR
                     ISPLYSNEKGKEHIIEGYKAETSIAVEISDLSSVAIIIDKAIEAGANIVRSVYYDRRD
                     LEAFKAQLIKSACIEASSKAEIAASALGFKLGAPIRVSVQDRFSAPDTGNMIMKASAF
                     GSESLRPGELEISVTVSAEFELMSD"
     gene            13727..14581
                     /gene="murI"
                     /locus_tag="PK105_04455"
     CDS             13727..14581
                     /gene="murI"
                     /locus_tag="PK105_04455"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOU60992.1"
                     /translation="MNEKIGVIDSGMGGLTVVRSLFLRNVPADIVYVGDNANVPYGNR
                     PLDEIVELTLRMLKVLSEHNIRIVAIACNTISATIERLKPYWVYPIIDIISPVAQFLA
                     NCGESDIGLFATEFTVRSGLHAKLVGEINPGVHIHGVPSRRLAALIDESIEDESAIRE
                     EISSMLAQLAALYPVHKVLLGCTHYPIVMDLFQKLAPEKEYIDPADLQAKAIIDLLNK
                     TELKSRSIENGREGVSTEIGKQAKGVLFSETAKPSLEIVTSGTEDTYRTMLSRLGIPK
                     ARSIDRLL"
     gene            14596..>15765
                     /locus_tag="PK105_04460"
     CDS             14596..>15765
                     /locus_tag="PK105_04460"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOU60993.1"
                     /translation="MEIERIDPFSYIIPVREGMRVPARIYASESLFSLLHEDKSIEQL
                     VNVARQPGIVKEALGMPDMHQGFAFPIGGVAAFDIDEGIVSPGGVGFDINCGVRVILT
                     SLDEKIVRPKLESIGKKLFARVPSGVGSKGVLKVSAKEARKAMDNGLRWTLAKGFAEN
                     IDLESVESGGYLEEADERAVSERAIDRGKEEFGTLGAGNHFLEISTVERIFDPARAKA
                     YGLFEGQVLIWIHTGSRGLGHQVATDYIARMRPRMTHYKIPLYEHDFVSIPISDPIAK
                     EYLSAMAAAANFAWINRQMITVAVREVFAQEFGEPWDSLGMRLLYDVAHNIAKFETHI
                     VEGMEKKLLVHRKGATRAFPAGHPELSLHAREVGQPVLLPGDMMRGSYILVGGPRA"
BASE COUNT         4502 a         3312 c         3784 g         4067 t
ORIGIN      
        1 cgcttgacag ctgcttctga gggtgctgca attatgcaag tacccttggg ggaataagca
       61 tgacccttcc gggaggataa gatgagccac gacaatatga accaaaaaat ctaattcttg
      121 ctaaaaagct atctatctat tttgattaat cttatatcgg tataattttt taattttgca
      181 ttttgctttt cttgtgcagt tttccgatgt agaatatgtg ttcgacagga gataaagatg
      241 gaaacgagat tgcttgcttg caaagtgctt ttaagccttc ttggggcagg aatctcaacg
      301 ggaaaggagt agaaagaaat ggaacctatc tatgaccaac agggggaaac tgttggttgg
      361 ctcaatgaca gtgttatata tgatcgcgcc aacaagtatc gagcattcgt gcaagacggc
      421 aacatcttca cgtacaaaag taaacacctt ggtaccctca atagaggttt ctttcgagac
      481 aagaaaggca actgtattgc gttcatgaaa ggggctaaag gtggaccaat cccaccgata
      541 ccggagattt cccccattcc accaattccc ccgattccac ctatacctcc catacctcca
      601 atcgcaccaa tcgggtcact taattggagc aagctgacat gggaagggtt cttgaatggc
      661 tgaaaggaat tttcgaacaa gccaaatcca gcgtatgcga taaatcgcgc cgctgatttg
      721 cggcgttagg ttgattccgc ttcgcggagc aaaaaaggag gggtttatgc ctatttctga
      781 cgtttctgta aaaggcagtt acattcaagt ttttgatgga gcaggaaaaa aagtctccga
      841 gatgtttgca tccggaaaag aggttgttgg aacagcttcc gatttctttg ttgtcgtaag
      901 cggatcgtat attcagactt acgatgaaaa atgcaagaaa attgcagaaa tgctttcatc
      961 tggaaaagaa cctcgtgggg catcaggcaa tagttttaca gtcaaatctg gaagttatat
     1021 ccaaacatat gataagaact gcaaaaaaat aagcgaaagg tttgcataag acattttcac
     1081 ctagcaactg cttcaacctg acaatgcctg ttgtcatggt ttgtgctgtc gcttcactca
     1141 gcacagacca cgccaagctt ttcgggccgg aattgcaggt taagccaatg taaaactggt
     1201 gtcataagcg cagaggagtt aatatgcaag tatgggtttt taattctcct gatgaaaagg
     1261 ctactgattt tatttattct tcattgctta aaggattttc tcgttttggc tggagctata
     1321 ttgatacggc agatttaaat atcttagagc ggaaacgatg ggaagagtta aatgatgatg
     1381 aaacagaatg ctatcgcaaa actagtttct tattgaatat tgaaatagga gattggatag
     1441 ttcacgtaaa tatacctgag tggggaaaat gtactgcagc acaagtaatt agcacatatg
     1501 aattcgataa agaacacaat tctattggta ttgattatac aaatttcggc gactatcgtc
     1561 atttaataaa aatagatgtt aatacattaa ttacttttga tagaaacaat cataatgtac
     1621 taccatatgt tagcagaaga ctgaaacttc agggacacta ttggacaatt aaaaaaacag
     1681 atgatttcct tcaatcgatt aataatctaa aatctaatgc tgtaaatttg gaaggtaata
     1741 ttacacatgg gctttatcac cttagaaatg atattagaaa gagtttgaaa gatatttcaa
     1801 ggatgattca agaaaaccat ccaggaaagc agttggaaga atttatagcg ctgatatttg
     1861 aaagagtgcc taatgtagtt gatgtaaaaa ggaatggttc tggttggggc acagattatg
     1921 gtgccgattt gataataact tataatactg gcataccaat acttgatttt caaacgtctg
     1981 aaatcattgt tgttcaagtg aaatcattta caggattgca tgacgatata aatactatcg
     2041 aacaaattgc aactgcaata gataagtata acgcaaagtc aggtattatt tttaccactg
     2101 ctgttgtaac agatagtttt attgctaaag tcaatgataa atcaaaatca ataggtaaag
     2161 ctattggaat tatttcaggt gatgatgttg ctagctttgt gcttaaatat ggctctgatt
     2221 tattatttga tatttagcct aacacacata aggcttggat agtcaagcag ttcctgaaag
     2281 aaaaaaatac agtgaccttt ccgggaatgg agaattcaaa tctcggatcc agctgcttga
     2341 ccgctgcttc tgagggtgcc acgattatac aaataccgta gcagaaatta gtatgacact
     2401 gccggggaaa tacgatggcc cccgacattt ataagggagg caaaaaatgg aatggcgcaa
     2461 ggcatattca aaagcaagca actttcttgc tgtggctctg atagcaacga tcttttcagt
     2521 aatcgtttca tgcaatattt tgggggaata taacaatcct gaaaatcatg gaaacactaa
     2581 ctactattct gtaacttacg atgcgaacgg tgcaacgagc ggcacggtac cggtggatag
     2641 caatagctat gcggcaggtg caacggtgac tgtcttgggg aacacggggg gccttgccaa
     2701 gacagggtat agttttgcgt ggtggaatac aaaagcggat gggacgggga caaactatac
     2761 ggcgggcggc acatttacga tgggatcggc gaatgtgacg ctatatgcac aatggattat
     2821 gcaattctca gctatttctg cgggctattt tcacaccatg atcctgaaga ccgacggtac
     2881 gctgtgggca acagggtata atggatatgg tcagcttggt gacggaacaa cgacaagccg
     2941 gagcacgccc gtgcaggtga tgtgcgatgt acaggcggta gctgtgagaa gcgatcacac
     3001 catgatcttg aagaccgacg gtacgctgtg ggcaacaggg tataatgaat atggtcagct
     3061 tggcgacggt accacaacga accggagcac gcccgtgcag gtgatgcgcg atgtgcaggc
     3121 ggttgctgcg ggagtctggc acaatatgat cctgaagacc gacggcacgc tctgggcaac
     3181 agggtataat tattttggcc agcttggcga tggcacgaaa actgaccgaa gcaaacccgt
     3241 gcaggtgatg agcgatgtgc aggcggttgc tgcgggtggc tgtcatacca tgattttgaa
     3301 gaccgacggt acgctgtggg caacagggga taatgaatat cgccagcttg gcgacggtac
     3361 cacaacgaac cggagcacgc ccgtgcaggt gatgcgcgat gtacaggcgg tggctgcggg
     3421 agtctggcac aatatgatcc tgaagaccga cgggacgctc tgggcaacag ggtataacct
     3481 taatggccag cttggcgacg gaacctggac gaaccgaagc acacccgtgc aggtgatgcg
     3541 tgatgttaag gctgctgccg cgggatacag acataccatg atcctcaaga ccgacggtac
     3601 gctgtgggca acaggctgca atagtgatgg cgagttcggc gacagcaccg cgacgtaccg
     3661 aagcaccccc gtacagataa atttttaatg ctcagtactt ccggtcgcgc catttctgga
     3721 caccaatcca tttttcaata atgcctcatc gaaaatctaa ccgaaacgct accgatgcct
     3781 catcatcaca gctaacccaa attacattca tggggctgga ggcatcatat ggggctcagt
     3841 atgcaagaac ggcatcgggt catcgcagaa accgcggcac ggtaccgtgc agcacgcaag
     3901 aaagaaaaat gccacattct tgatgaactg acggcattaa ccggctacga ccgcaaatat
     3961 gccctgcacc tgctcaccta gtggggtaag acagtgtcga gagagcggtg ggaggcacac
     4021 gccttatgat tgtcattggc actgtccagc atcgcaagaa gcgtaccggc aagaaaaagt
     4081 attcgcagga gctctacgag gcactgcagt gcatatgggc aacttttgac tgcatgtgcg
     4141 acaagcggct tgcagtcttt atccgcgaaa acatcgcttt ccttgcacgc cacgaagagt
     4201 atgccattac cgataccctc cgcgcacagc ttaccaccat cagcccgcga catgactact
     4261 gccaatgatc tgccaagaag caatacagat tacctctgct atgacatcaa caaatctcat
     4321 catatgtaaa actcaaatag ctggctttcc agctcctcag atggtttgca tttcagtgct
     4381 tggcacagta gtataacctg atttttgagc atatctttga gtcgatataa gatctacttc
     4441 gttttgctat aaatgtggct tagtttttta gctcacttca gagaaatagc tactaagacc
     4501 ggatggttgc caaggcaacg atgaaagaga actggacgga ggagtggggg atataccggt
     4561 ttgacgacgt ggaaggattt gggaataggg tagtcaagcc tggcgacata cataagcgac
     4621 cactatcctg tctgggcaat tttttatgct gataaagata gcgattagta tgccgcttct
     4681 aagcttaata tggggaaatc cgaatcttgc attgtataat ttgcgggtct agggtttatc
     4741 attctggcat ggaagatgaa gaaacaaaac ccattttgct atcaccgatt gaagtgcgcg
     4801 aacttgaagg catggcgctg gcaatggaga gcattgcgaa agggatccgc gacattctgg
     4861 cacgtgggca agaaggcgaa aaggattgaa tgctggggcg aaggcttcag ccctttttgt
     4921 tatctagagt atctgctttg caagtgaaat ataggacggc aggaagcgca aaaaaggctg
     4981 caaagggctt ctgaagcggc gggtgataaa aaaagagaca cagaggagaa taggaaacgc
     5041 gtctatggcc tgccatttca aaataggggc aaaacaggga tgtgttctct tgacgatgcg
     5101 gtaatagcgt acaaaaataa aaaagcagag gataaggtta tgaaaaaata cttatttgtt
     5161 tttgcattgc tgctttgtgt aggaagtctg tttggacaag acctaggtgc agaggtaaat
     5221 gccagggata aaaatggcaa gactatcttg atgtatgcag ccatgtatag ccagaatccc
     5281 gaatctatta ccgcttggct caacgccggt tcagaggtga atgcacgcga taaagacggc
     5341 agcaccgctt tgatgttggc agctgggttt aatcagaatc ccgaagttat taccacactt
     5401 atcaaagccg gcgcaaaact ggatgaccgc gatacggctg gccggactac cttgatgtat
     5461 gcagccgaac taaattcgaa tccagaagtc attactacgc tgctcaaagc tggcgcaaaa
     5521 ctggatgacc gcgatacgaa tggcaagact gccttgatgt tggcagcttg ctataacccg
     5581 aatccagaag tcattactac acttatcaac gccggcgcag aggtgaatgc ccgagaagaa
     5641 atattaggct taatgtcttt gatgtgggca gccgcaaata atccgaatcc agaagtcatc
     5701 actatactta tcaacgccgg cgcagaggtg aatgcacgca ataaagacgg cataaccgct
     5761 ttgatgttgg ctgccatgaa taatccgaat ccagaagtca tcactacact tatcaaggct
     5821 ggcgcaaaac tggatgaccg cgatacgaat ggcatgactg ccttgatggt ggcagctcac
     5881 tttaatccga atccagaagt catcactata cttatcaacg ccggcgcaga tggaaaatta
     5941 aaatccagca aaggcaaaac cgcctttgac tatgcaaaag ataatccgaa gattaaagat
     6001 accgcagcat actgggcatt gaatgatgcg agattttaat atttagaaga aggctacaag
     6061 caataaagat taaagtggaa ggaattaact tccactataa gcctaagatc aaattgatat
     6121 ggaggcgaag acattgcgtg agcttgacag aagcgatatt aaagccatga tcgaggatgc
     6181 tgaccggatg gctaccggct ggatgtgatg aacaaaaagc ggcttactga tgatcaggga
     6241 ctcaagatgg aactcgcggg ggtgatgtgc cggctttctt cgagcgtatc gatccttgcg
     6301 gggaatctag ttgtgagcat cgaggttcag cagggagaga gtggtacaat ggcggtcaag
     6361 gacgaggtag atcatacacc gccgcgggcg ctgacgaatg ccagcgcttt ttttgtgcgt
     6421 gatgagcatt cgcaaaggca gaaaatagga ggaaagggtt cttttttgca ggttttgtag
     6481 gaaatatgta ggaaacctta tttttatgtg cgaaatattg catactatca aggaaatttg
     6541 aaactacagc aaggcagagt cccccgtctt ggctactatc atgcgggttg tgtaaagcag
     6601 actggtaaaa ttgagagcat tgtaaaaggg attcgcgaca ttctggcacg agcacaagcg
     6661 agcgagcatg aatgaaggcg caggcttcgg ctgccacttt tctttatgca aagcgcaatt
     6721 ttacactatg ccacttgcaa agcggaaccg ccacaccaac cgccacaata ttatctatat
     6781 atgcgatata aaattatgat acttttatgg cggattggcg gttgaagaga ggagaaagga
     6841 gctacgcact catggccggg tactagaaag catgacgaat ggtattcgcg acattctgct
     6901 tggcagctac ttctgatggt gttgtaacta tgcaagtacc cttgggggaa ttaccatgac
     6961 catggacatc aaagtgatat cgaaaagtga catttttgtc caaattaaac tgaaagtgac
     7021 attgaaaagt gatattttga cttctttacg aagatgctat ctgatgatat tatattagtt
     7081 atagtgatat agaaatacat atagggcaat taaatataat tgtcttatat gctttgaatc
     7141 acattatctg atgtcgtatg atatctgctt gatggtgaat tcaatagctt gaggtgctag
     7201 tgccgagagg catggaggtt caagtcctct tcgccgcaga acgaattatc gtttgtcgat
     7261 tatgcttaaa tcgttgaatg aaccaacctg gatgacgaat gcgggcacga tagcggctgt
     7321 atcatcaata tttccgagaa cgcttgcgcc taggaatatg ccgaaatgcg gcgctatgct
     7381 cacgctcgct ccgagtgttg cgctccactt gagtggccac atcgcatagg ttgatgggtc
     7441 atcgtattgt gcaaccgcgc ccgcacttcc atttgcaaaa atatatatat cgccacctgt
     7501 caattcattg ataaccctta tgcgatgccg gaattcaagg cttccagcaa gcactgtatt
     7561 ccccgaacag tcatcttggg tataaatcag gccgtagaac ataagcggct ggttcagtgt
     7621 cgaataatag gaaattttcg ccggctgggc tttatccact aaaccagcaa aatctgttcc
     7681 accaaaaagg ctcagatgca gtgcatcggt ttgcccaaca ggaatggcgg ccgaagcatt
     7741 gatctcaatt tgtgaaaacg agaattggcc gccaaaattg gggctgaacc agcgcccaaa
     7801 cgcgttgaga gagagcccgt tgcgagggaa tacggttctg tctcttgtgt cgagtctcag
     7861 cgcggctctg agcgcgcccg cgttctgcgc tgccaggttt gtccagtcat cgccagtgag
     7921 cacgttttcg aacgagtagc cagccatgat atccccaaca tttcccagcg caaggccgcc
     7981 ccatgcgcca gcaccgtatg ttctaaattt cgaagctatt gccacaggca aggattcgga
     8041 cacgaacatg tcatattcaa agcgaaagcg agcccagggc aggatgaaaa aagatccgaa
     8101 aggctgaaaa tactctaggc ttgccgaggt tttgttcaca aaggagaccg aagaaaacag
     8161 cgcagaacca ggccctgtga gctccttggc cagaaatgcc aaggatgcca ccaaattgct
     8221 cgaaatatca ttcgaaatgg cgctctcaaa gcccaaggag gcaaagagtt cgtcttgcgt
     8281 aggtttttgc ggcacaatgg atacaacgcc gcgcagagat tcagactgtt cgccatcctt
     8341 caggctttct aggtcgaatt tgacgagcga gtaatcgcca gcaataaatg cagcatggag
     8401 ggcattctca agggcctctc ttgaataagc cttgccaatg agcggagcaa aaatgccggc
     8461 aattttatct ctatccgatt ccgagcctcc ttcgaccctc agcgaggaaa acatcggagg
     8521 tggggcgaaa gccttccgat tggcctgctc agaaggagat accagggctc tctgctctcc
     8581 gatttttgta gcaagttcgc gcaattgcgg cattgcgata tcgccggctt cttcgccacg
     8641 ggcgataatc tcttttgcgc gtgcaaagct cgcggttgta aattcctcga gattcggagt
     8701 gataacgagg tctgctgctt cgagatttgg cctcatgttc tgcatgatga aaaggttgcc
     8761 tgtttggctt gctatctcta cagaggtgtt cagatcatcg atcgaatcag gagccttacc
     8821 tctcgaaact actgcaatga ctatatcggc gcccatctct tttgcgatat tgacgggcaa
     8881 attatcgacg atacctccgt ctaccaaaag ctggccatta tgttcatacg gcatgaagac
     8941 aacaggaatc gacattgagc tgcgcatcgc ttccgcaaga gagccttggt cgaacaccac
     9001 cgtatcgccg ttgagaatgt tagcggcgat tgcgcgatag ggaacgggga attcgtcgaa
     9061 gttccgtttt gttgccatgt gaatggtacg cgcagtgaag aatgcaagga tattttgccc
     9121 ctccagaagt cctgcgccta tgtttggtcc ttttgagtcg aatccaattc tgagcgcata
     9181 catcgattcg actgctcgtt ggaacatatt ctgtggaagg gccttagagt ccataaagag
     9241 cgaattccag tcgatgtcgg atataagctc ttgcatgtcc tgcggcgaat aaccagccgc
     9301 atagagaccg cctacaattg agcccatact cgtgccaaga atcatatcga ttgggatgcc
     9361 tgcttcttca atcttttcga gaacacctgc gtgggcaatg ccgaacgccg agccgccgga
     9421 gagcaccacg gctattttgg ggcgcggcgt ctgtacctca gagccgtctg cgaccgctgg
     9481 ccatgtaaca gcaaagcaaa ttaaaaggct cacaaagcca aggcgcgcca ggcgccagag
     9541 aggaaggtgc atccaattca ttttcataag gagcatcata gatactagtc tcttgtttgc
     9601 gcaagcctaa atgcgcaaga atgccactat gccccactat ctttattctt aaaaaacaaa
     9661 gaggcaggtt ctgtgtcctg cctcttttgt gtagaatgat ttttacccct ctctatgcgc
     9721 cgcggacatg cggctgactt ttagtcttgc ttttctccat aagtgagcat aacgccatct
     9781 agagggcctg ggtcaggctg accatcgcta tcgaattcaa caaagagctt gccgtctcct
     9841 gtcaggaaga actttactgt gatagtctta gcataatctg attctgcttt taggtttcct
     9901 ccagcatctt ttatatcata gttatatgag tattgataaa tcatgccttc agagcttgca
     9961 aattgtccat catatttgca agtataacca tgattatcaa cacctgtaaa atgcattgtt
    10021 tcgatggagc tttctccaat gtgctctgta tagatattca acgtacaagt tgtaaaaaaa
    10081 ctaaagccta ccccttctgg ttgattcggt atccaaacat ttgtaaacgt ccaatttcct
    10141 tctacctgcg aatattccgc aggcgtcaat actggagact caccttcgtt ttcttgattt
    10201 tcgccacctt caaatagttc aatagtgaac gagccgagca ggccatcagc ttgtacctgc
    10261 attcttaatt tgccgtcaat taaggaaaaa gtgatttcta tatcttgagg ctcatcatac
    10321 tgatggatgt tgccttgatc atcctttact ccataggtgt attctccttt atatacattg
    10381 tcatcaaaat ccccgtcgct ccaataataa gaactgtcgc cgatagtaat gtctactctg
    10441 ttatgatatt gcgattctgt accatgagcc cctttttcaa taaaaacatg tacaggatta
    10501 taaaccttgc cattaatttt cacattgctg aaatcccact cgcctttgat atcatcgatg
    10561 gtgatgtttg tgttttctgt atctgggggg ttgattgggc aacctaccag aaatatcgct
    10621 accactagca agcctaacca tagaaaccgt ttcatcgcct cctcctttat cnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgccgcaa tctagtttgg tctgcacaag
    10801 cttattatcg ctcctgaatg cagaaaccgc gggacgctgg gaatgggcgc gaattatggt
    10861 ttcctcatct tgtcagagta acgaaatttg ccattgcatc ctggaaaatt ctgcattaca
    10921 gtctattcac ccgatcatcg aatcaaagaa gaggaaaatc accgagaatg acaacaatcg
    10981 tcctttcggg aggcggtggt actaggctat ggccgctttc cagacaagct attccaaaac
    11041 agtattgcaa tcttttaggc ggacttacgc ttttcgagcg cacaatcaag agaaatatca
    11101 agttgtctga taacatcata gtagtcacca atgtcgcgca tttcgatata gcaaaaagtc
    11161 agtttagaaa agtctgtcct gataaacagg tccattttat ccttgaacct tgtggccgca
    11221 acacagcgcc ggggatcgca ctggcctgtc ttgagctaga tcctgaggag aacgtgcttg
    11281 tcgtgccatc cgatcatatc atattggatg aagaagccta tcaagatgcg gttaatagag
    11341 ctcttgtggc agcaaacctt ggataccttg taacctttgg gctaaagcct gaatatgctg
    11401 aaacaggttt cggctacatc gaagctgatg gagataaaga gaccgcgggc gaaggttttc
    11461 tgcttgtaaa ggcctttaag gagaaacctg acagagctac cgccgaagaa tacattgcag
    11521 caggaagata ttattggaac tccgggatat tttcatttct ggctggcaaa tacctttacg
    11581 agctgggcaa tacggcgcca gagatactct tggcatcaaa gaaagcctgg gaggcagcta
    11641 gaaaatctat ttcccaaaat actgaagatg taatagccat tcctgaggaa gcgatgcttt
    11701 cgatacccgc agaatccata gactatgcag tcatggaaaa aagccagaat gttgcggtgg
    11761 tgccttgttc gctgggctgg agcgaccttg gaagctggga cgctatctgg aagattcggg
    11821 agagaagcgg cttagaagct ctagaaggat cgtcgactat ttccattgag tcaaagagaa
    11881 acctcgttgt gggctctgaa cgtattgtcg cccttgttga cgtagaggat ctgattgtag
    11941 tcgatactcc cgatgctctt ctgatttcaa agcgcggatc gagtcagaat gtaaagagaa
    12001 ttgtcgagca gttaaaagag aaaggcaaga ctcatctctt gtaaaaaaat cccaaaatcg
    12061 atgtaaagaa taattaccta agcatctgtt ttgttatcaa ttatttctta cacaataaat
    12121 aatcttttat atattgactt atcatcttgc ttccttcaaa ataaaataaa aatatgggaa
    12181 gagaacgtct ttttgccgat aaaaaagaaa ttattgacgc cgcaatagag cttctaagcg
    12241 agaatggata tgagagtttc tctacacgaa agcttgccag gagacttggt gtatctccca
    12301 tgacgctata taattacttt tctagtagaa atgaaataat cgaagctgcc atcgaagctg
    12361 cctatgagag agtgtttcaa gaattctata atgatctttc atcatatctt gaaaaagagt
    12421 ctttctgccc tctctgtggc tttatggaaa taggcaagaa actactattg ttttctcgtg
    12481 aaaagcccaa gatatatgcg ctcgtattta taatgaatat catgccatat cgcgaacaac
    12541 ccagtgtaca aagacttttt ggtattactt tatcaaaggt tcgagctcgc ttaactgcgg
    12601 aagaaatcag ccaggatctt cagaatcata tctacctttt tatgttgctt gtcttagcac
    12661 tggtacagaa tgtgtacaat gggaatagct cgtacgatat ccgagctttc gaagccaatc
    12721 ttcggattgc gtataatcag cttctaaaac ctttcgaaaa atatttttca tttgcataag
    12781 catagcaggg tacaaagtct gctttattcc tatacacaag gttcgtagca gtatagtagc
    12841 agcaacagct atatcaaaga cctctagatc aacttataat agcaaaggag agtagactat
    12901 gggcttattt caaaagaaaa aacactttct tgcctttatt ccttgcatct ttgggcttgt
    12961 catatggtct ttaataccca ttgcagcggg tgctcaatcg gaaccaggcc acagcattca
    13021 ggtgtatggt caagcgtcga tgtatgttga gcctgatgtg gccaatttgc ttatcgggat
    13081 tgaaatacaa gccgatagcg cagcaaaggc tcaagagcaa cttcgatatc aagcatcagt
    13141 ggttttcgac gctatatctc aagcaagtat ccagcaaccc aagatgaaga ccagttctta
    13201 ccgtatatcg cctttatata gcaatgaaaa gggcaaagag catatcatag aaggctataa
    13261 ggcggagaca agcattgcgg tggagatttc ggatctgtca tctgtggcaa ttatcatcga
    13321 caaggcaata gaagccgggg cgaatattgt acggtcagtc tactatgatc gtagggattt
    13381 ggaggcattt aaggctcaac tcataaagtc agcctgcata gaggctagtt caaaggccga
    13441 gattgccgcc agcgcgctag gctttaaatt gggagctccc ataagagtca gcgtacagga
    13501 taggttttcc gcccccgata ctgggaatat gattatgaag gcgtcagcct tcggctcaga
    13561 gtctctaagg ccaggagaac tcgagatcag cgttacagtg tctgctgaat ttgaactcat
    13621 gtcggattag caaggcagtg cgataattga gctattatat aaggaagaga tttttgctct
    13681 atggcattcg attgacagaa aacaatagtc atgtatcaat gtattcatga acgagaaaat
    13741 tggagtcatc gatagtggca tgggcgggct cacggttgtt cggagccttt tcttaaggaa
    13801 tgtgccagcc gatattgtct atgtcggtga taatgcaaat gtgccttatg gcaataggcc
    13861 attggatgaa atagttgagc tcactctgcg catgctgaaa gtcctttccg aacataatat
    13921 tcgaatcgta gcaatcgcat gcaacactat ttccgcgaca atagagcgcc tgaagcctta
    13981 ttgggtatat ccgataatcg atataatttc tccagtagcg caatttctgg ctaattgtgg
    14041 agaatcggat ataggcttat ttgccacgga gttcaccgtc agatcaggct tgcacgcaaa
    14101 attggtaggt gaaattaatc ctggcgtaca cattcatgga gtaccaagcc gcagattagc
    14161 tgcccttatc gatgaatcga tagaggatga atcagctatc cgtgaggaga tatcatccat
    14221 gcttgctcag cttgccgcgc tttatccagt tcataaggta cttcttggct gtactcatta
    14281 tcctattgtt atggacttgt tccagaagct ggcaccagag aaggagtata tcgatcctgc
    14341 ggatctacaa gccaaagcca taatcgacct attgaataag accgagctga aatctagatc
    14401 tatagaaaat ggaagagaag gtgtaagcac tgaaattgga aaacaagcca aaggagtcct
    14461 ttttagcgaa accgcaaaac cttctttgga aatcgtcact tctggtacag aggatacata
    14521 tcgtactatg ttgagcagat taggaattcc aaaggcccgc agtatagatc gtttattata
    14581 gaagcagagg ggcatatgga aatcgaacgg atagatccgt tctcctatat aatccccgtt
    14641 agggaaggaa tgcgtgttcc tgcacgcatc tacgcttctg aatcgctttt cagccttctt
    14701 catgaagata aatccataga gcaactagta aatgtagctc gacagcccgg aatagtaaaa
    14761 gaagctcttg gcatgccgga tatgcatcaa ggttttgctt ttccgatagg aggcgttgcc
    14821 gcattcgaca tagatgaagg cattgtatcg cctggcggtg tgggattcga cataaattgc
    14881 ggtgtaagag taatcctcac ttctctcgat gagaaaattg tacgtccgaa acttgaatcc
    14941 attggcaaaa aacttttcgc acgcgtgcct tcaggagttg gttcaaaagg ggtgttgaag
    15001 gtatctgcta aagaagctcg caaggcgatg gacaacgggc tgcgctggac tttggctaag
    15061 gggtttgcgg agaatatcga cctcgaatcc gttgaaagcg gaggatatct ggaggaagca
    15121 gatgagcgag cagtaagcga aagagctata gatagaggaa aggaagaatt tggaactctg
    15181 ggggcgggca accattttct cgagatatcc acggtggaac ggattttcga cccagcacgc
    15241 gctaaggcct atggcctatt cgaggggcag gtgctcatct ggatccacac cggatcaagg
    15301 ggtttaggac atcaggtcgc cacagattac atcgcgcgca tgcgcccgag aatgacacat
    15361 tataagatac ctctctatga acatgatttt gtcagcattc ctattagcga tcctattgcc
    15421 aaggaatatc tttcggctat ggcagcggct gcaaactttg catggataaa caggcagatg
    15481 ataaccgtcg cggtgcgtga ggtttttgcc caggaatttg gagagccctg ggattcgctt
    15541 ggtatgcgac tattatatga tgttgctcat aacattgcaa aattcgagac tcatatcgtc
    15601 gaagggatgg aaaagaagct tcttgttcac agaaagggcg ctacgcgtgc ttttcctgct
    15661 ggtcacccag agctatccct acatgcgcga gaagttggcc agcctgttct tttgcctggt
    15721 gatatgatga gaggcagcta tatattggtt ggcgggccta gagcc
//
LOCUS       DAOOWY010000038        15755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3747_length_15755_cov_9.086115, whole genome shotgun sequence.
ACCESSION   DAOOWY010000038 DAOOWY010000000
VERSION     DAOOWY010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15755
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3747_length_15755_cov_9.086115"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK105_04465"
     CDS             <1..543
                     /locus_tag="PK105_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOU60994.1"
                     /translation="AAASSKPVIVYNVPGRTGLNLAPSVLKQLWEIENIVAVKESSGN
                     LAQIGEICRTLPEGKTVLSGDDALALASISVGAEGLVSVAANVLPRRFRALVDAARTG
                     RRQDAMAMQRELLPFMDALFVESNPIPLKTALKFCGLTGDRLRLPLVSAARATKELME
                     SVLAALGCGYGYGRCDELLQ"
     gene            509..1369
                     /locus_tag="PK105_04470"
     CDS             509..1369
                     /locus_tag="PK105_04470"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOU60995.1"
                     /translation="MDMGGVMNSCNEMNSGAEVNNAAQILEGIFLEQGRFDREQWALF
                     LELLENGKIRVVDRLLDGSWKVNAWVKQAILSGFRAGGIAEWSWPAEGFYDRPAFPPR
                     HFARSDGVRIVPGGSAVRRGAYIAQGVVIMPPSYINTGAFVDQDTMVDSHVLVGSCAQ
                     IGKKVHLSAGAQIGGVLEPPQANPVIIEDEVFIGGMCGIFEGILVKKRAVLAPGVIIT
                     KGTRIFDLVRERELYGEVPENAVVVPGARPARGSYAKDRGISLAAPCIVKYRDEKTDS
                     AVILESALRG"
     gene            1381..>1785
                     /locus_tag="PK105_04475"
     CDS             1381..>1785
                     /locus_tag="PK105_04475"
                     /inference="COORDINATES: protein motif:HMM:NF022187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOU60996.1"
                     /translation="MQYKLGIIGAGKIGEAILAGSLSKGLLTPSDVILSVRTEQHRKN
                     LEDRYNVTAVLRNREVARQSEIILIAVKPRTIFDVLQEIADIISDESVVVSTVAGVTL
                     DSMQKKLARKVALIRTMPNLGVLVGEGMTALAP"
     assembly_gap    1786..1795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1796..2189
                     /locus_tag="PK105_04480"
     CDS             <1796..2189
                     /locus_tag="PK105_04480"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HOU60997.1"
                     /translation="EESKIQKVKQLFESVGRALVLDEQYMDAVTGLSGSGPAYVYLII
                     EALTDGGVKMGLPREVSIELAAQTVLGAAKTVLVTGEHPAKLKDQVTTPAGSTIDGLM
                     ELEDGGLRVTLIKAVVKATERANKLLQK"
     gene            complement(2311..3459)
                     /locus_tag="PK105_04485"
     CDS             complement(2311..3459)
                     /locus_tag="PK105_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60998.1"
                     /translation="MLIERLAKMYVQWGAPKQSIESDRRRNRQIQAVREFLEIIQKTG
                     ENRKELFEVSQQSKRKATKLSYLLLASDTEPAAVRDSFLRSGLLFKGQQNNKPELLMW
                     LAGTEACARGIHPYIVFLIMSAYSGLEAANSEFLWLRERSKLQNAKLTEYIVPGDLTD
                     NIEEALSEPLRFKRTIRIAGMPLAASAFAGCSLYYIEQILALVGPIGSFILTEMIAAA
                     RKRLISDEICTAQQAFLDLYSRQESESSALDKGLMTGMEAFEESSQPDPDLIRTTTDI
                     IMSAEAKILKATLSSMSDNEIASLLRCIEPIAHERLLSLISQGRQRRILTAIQHTDNV
                     TGEKMLRDAQLFAQKLLSSYAPRNLRPGETLKIPELLRNLISSLLGRE"
     gene            complement(3449..3865)
                     /locus_tag="PK105_04490"
     CDS             complement(3449..3865)
                     /locus_tag="PK105_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU60999.1"
                     /translation="MIYWNADLAAKIACSSEDMKILPSYIDYLVDSAKKIAQGVMLPD
                     SEQLSSEKDDFFRYGLLLVSEGLSGEILEEILAVLLYVSKVEGIEFLKQCVAAEAILS
                     IANGEDEEIMIRKLLPYCGIDAALDTVAQRKSEHAD"
     gene            4217..4981
                     /locus_tag="PK105_04495"
     CDS             4217..4981
                     /locus_tag="PK105_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOU61000.1"
                     /translation="MILAEFDNWARSFLEIDQLRQIDDSLNGIQIACSPSKPIQKMAV
                     AVDACAESIRRAHNVGADLLFVHHGLFWGKPEPIIGSLRERLKILIDSDMALYACHLP
                     LDKHSEVGNNARLADLLELKDRKPFGIYHGISIGWSGVLPKPAKLDKIIAKILPDGSA
                     AKSVIAAGPKEINTVAIVSGGAPFESLEAIREGIDLYITGEPSHSIYHYLIEGGINFI
                     AAGHYATEIWGVKAIAEKAHAELGLDTIFLDIPTGL"
     gene            4983..5519
                     /gene="lspA"
                     /locus_tag="PK105_04500"
     CDS             4983..5519
                     /gene="lspA"
                     /locus_tag="PK105_04500"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOU61001.1"
                     /translation="MKKNVPLVPILFSGIIIIVDQITKALVVKYIPEGRVFARILGDF
                     VWIVHTRNRGAAFSIGALSPQPLRFLFFICLPIVVLIALLFYYFKSERLSPILRYSIG
                     LIVGGGIGNLIDRILRPQGVVDFISVNMYGFLGMERFATFNIADSAITIGEILLILGF
                     LILEFHKPKAQSSQSEKG"
     gene            5528..5803
                     /locus_tag="PK105_04505"
     CDS             5528..5803
                     /locus_tag="PK105_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61002.1"
                     /translation="MNKKQANKGKVALFIVGATVANILLMAICFVLFMLLYSVAFSKF
                     LPQEALIWAIGIAFLLSLLVSSLIYRRLLKLLRERYHLDDYLGLKAK"
     gene            5852..7210
                     /locus_tag="PK105_04510"
     CDS             5852..7210
                     /locus_tag="PK105_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOU61003.1"
                     /translation="MRERLRDFGISVPEIYLPRQGIDYRKWAVIACDQYSSEREYWED
                     VAREVNDAPSTLRLIFPECFLEDSDIDQRILAVHRTMHEYLEQGLITSRGEAFVLVER
                     TTCLQEKPRLGLIVAIDLEQYQYSEDLKTLIRPTEATIKERLPPRMRIRRGAPLELPH
                     IMLLVDDPKKTLIEPLYERRYYLEKAYDFDLMQNSGHIRGWFVREDREFDRIAQALEN
                     LSDPCTFRARYSHDNGADDVLLFAVGDGNHSLATAKAIWEEIKNAYRDKPDAQRILSE
                     HPARYALVELVNLHDEGLPFHAIHRVLFNIDASDFLRMLSAVGAEIQYRSDARQAFAE
                     VDSEQDSHIFAFVTENKSGTIRFDKPKTLLAVSTIQEYIDEYRKVHPGFGIDFIHGGT
                     SIETIGRKNGNMGIYLPPIKKESLFSIVIHEGLMPRKTFSLGEASEKRFYIESRIISP
                     QQ"
     gene            7329..8201
                     /locus_tag="PK105_04515"
     CDS             7329..8201
                     /locus_tag="PK105_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOU61004.1"
                     /translation="MLILLADAFTPDLPERLAEFGEVTSDMSRVAEAEVLIVRSRTKV
                     DGAMLDRAARCRYIVRGGVGLDTIDAEYARTKGIIVDNTPEASSIAVAELAFAFMIAM
                     PNHLVRADISMKEGKWLKKELERSELYGKTLGLVGNGRIGRELAHRAKAFGMSVIAFD
                     KYVMSSDIVRIVCFDELLALSDYISLHIPLIDETRGMIDASIISRMKNGVYLINTCRG
                     QVVVEKDIAEALKCGKIAGYATDVYSKEPPEGSPLFGAPNVLMAPHIGASTEENLLRL
                     ADCIVARLKKYVGR"
     gene            8218..9315
                     /gene="serC"
                     /locus_tag="PK105_04520"
     CDS             8218..9315
                     /gene="serC"
                     /locus_tag="PK105_04520"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOU61005.1"
                     /translation="MKRVINFNAGPAALPMEVLRKAQEEMLDWQSTGMSVMEVSHRSS
                     EYEAMHNESQELFRKLAGMGPEWKILFMTGGASTQFFMVPMNYLFGKRKATYVVTGHW
                     SKAAKREAQHFGSVDVISTENKDGSFTRIPKQEELKIDSSSTYVHMTSNNTIYGSQWQ
                     YWPELEGVPLVCDMSSDIFSRPFPVDKLALIYAGAQKNLGPSGVTVVAIREDFLDIAR
                     EGSKLPTMLSYRTFSENNSLYNTPPCFSIYILNLMLKWLLYRIGGLQKMQEINEEKAR
                     ILYEAIDLSDNFYRGPVEKGSRSQMNVVFRLKTPEMEDTFIEKARSAGIVGVRGHRST
                     GGIRFSIYNANLVDNVRKAAEFMEEFQRINR"
     gene            9419..12205
                     /locus_tag="PK105_04525"
     CDS             9419..12205
                     /locus_tag="PK105_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61006.1"
                     /translation="MAKSRLHRLYNKFISSLSFSAELRKMRRELNVKIDQPESITAPP
                     PFHPQAANRWFKRRRISIAESYLMVVRDLDSRNSSRRLTALKNLADVAFRSSSIDYPL
                     NTARVQSALVKEVVKHRSNKRRQLELLYDFSMSTQGQHQVIRKLCDELNIIELPENGM
                     KIGELGYAWDDHVHDVATSGRKNPTQLVLDAFIKGISSLTVAYGLVSDIDLMEEVLEA
                     GNILGLKVNIGLEFSVLVEGARYHFIAELPHFSSKEELRSFFSDHALDMDKFFKGLEI
                     NRESRLDAVRRLLENFNQNTLPKINEGFENKPEYCLAPLSLEVLLATIPNVNITPLHL
                     AEFMYVRYRPILQKRVWYYKVLREKARYIAQNPSGLQQDQTNGELQREEIEASYSELK
                     KELNRLSPDLVLSAYFDSPHIISYQSAFDDLESISQMLKRAGCSIRFVQPLEHGLELA
                     KQALIKWSRCIDAVEIYNIQDCIGRNPESIEDFARFVNELNKSAAAEGRSFLKPICGS
                     DATGRNPKIPGMGFIFEDQIIGNLRKRYIKRHMRLPSLISAMIRSSESPVDEASLQNA
                     AVPSIVCMGKVSGSWNRAITGDEERINPLRAWRYFNPTFKNAIRAIIGFTVATAYIGP
                     AYALLWLGITGFRNSIADLISYRGPKLSQWKLKSINFDNVGQSLFWTGFSVPLMGYAK
                     STFDILWPLAPETFLFNLVKFFVISIVNGFYLAAHNTLRGFDKNVVRANLFRSILAWP
                     LATIFAPIGNSLSIPSIVQTKIWSDVVAGFIEGGNKYRKVLQLRQKTLEELIPTVINA
                     SGNAQYIATMDVLYLFSQEPRTRTTIKAILSPYAFFTRKLKENSSLRLNMLLEFNDKM
                     SKESLWTDLVDYIVANYEEDMADDLVDLVVDGLPELLGDLSGLIEKYGKDPSLLAKLA
                     AKASGTIRGSGQAK"
     gene            complement(12427..13533)
                     /locus_tag="PK105_04530"
     CDS             complement(12427..13533)
                     /locus_tag="PK105_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOU61007.1"
                     /translation="MDNEVTWWKFDEVDAFMRAGFEAVGVAPEVATVCSDVLISADKR
                     GVDSHGVGRYKPIYLDRIWAGILDPRTTFDVIRETPTTAVIDGHNGMGLYIAKRSMEL
                     AIEKADKFGIGMTVCRNSTHYGAAFYYARQAVEHGMIGMTTTNARPSIAPTWGVEPML
                     GTNPMTWGMPSDEDFPFMLDCATSVTQRGKIELYDRLGKDLPDGWVIGQDGKYRHDTH
                     QVLIDLGQDKAALTPLGGLGEDFGGYKGYGYAMVVELLSSALSQTNFMKALLGVDSNG
                     KPKPIELGHSFIAINISAFCDLEDFKHHVGEVSRQLRASRKAPGAQRIWTPGEKEHET
                     WLYRKDKGVPFNPPLKKAFKEVKERCKLDIELPF"
     gene            13782..>15755
                     /locus_tag="PK105_04535"
     CDS             13782..>15755
                     /locus_tag="PK105_04535"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOU61008.1"
                     /translation="MSAHPYTITLDFENLPAELVFEPGVRRAPKRDAELTPSQERIAL
                     KNALRYIPARWHERLAPEFLAELRQRGRIYGYRFRPQGRIIGLPIDNYSGRCIEGRAL
                     RVMIDNNLDFDVALYPYELVTYGETGQVCQNWMQYRLICKYLEVLDENSTLVVESGHP
                     VGIFASHPFAPRVIITNGLMVGMYDNLKDFNIAAAMGVANYGQMTAGGWMYIGPQGIV
                     HGTYNTILNAGRLKLGISQDKDLSGRLFVSSGLGGMSGAQGKAAMIANAASIIAEVDY
                     SRIETRLKQGWVSCETDSCEEAVHLAHAALDKGEPIAVAYHGNIVDLLEYIDSHDIHV
                     DLLSDQTSCHVPYDGGYCPVGISFEERTRLLAEDRHYFRALVDASLRRHFEVISRLVK
                     NGTYFFDYGNSFLKAVFDAGVKEISRNGIDEKDGFILPSYVEDIMGPELFDYGYGPFR
                     WVCLSGKKEDLHKTDLAAMSCIDPDRRGQDRDNYIWIRDAEKNKLVVGTQARILFQDA
                     FGRMNIALKFNKMVRNGEIGPVMIGRDHHDTGGADSPFRETSNIKDGSNVMADMATQC
                     FAGNAARGMSLVTLHNGGGVGIGKAINGGFGLVLDGSEKVDQVIRMALPWDAMGGVAR
                     RSWARNVHAMEVADQFNCEYGEYGTITMPHLVDE"
BASE COUNT         4132 a         3438 c         3918 g         4257 t
ORIGIN      
        1 gctgcagcga gctctaagcc tgttattgtc tacaatgtcc cagggcgcac cggcctcaat
       61 cttgcgcctt ctgtgctaaa gcagctttgg gagatcgaga atattgtggc ggtcaaggag
      121 tcctccggca acctggctca gataggtgag atttgtcgca ctctacccga aggaaagaca
      181 gtgctatccg gcgatgatgc tctcgcgctt gcttctattt ccgtaggagc agaaggtctt
      241 gtcagcgtag ccgcgaatgt actgccaagg cgatttagag cgctggtgga cgctgcgcga
      301 acagggcgcc gccaggacgc gatggctatg caaagggaac ttctgccatt tatggatgcg
      361 ctttttgtgg aatcaaatcc cattccacta aagaccgcac tcaagttttg cggactaaca
      421 ggagatcggc tgagactgcc gcttgtatcg gcagcaaggg caacaaagga actcatggag
      481 agcgtcctgg cggcgctagg ctgcggttat ggatatgggc ggtgtgatga actcttgcaa
      541 tgaaatgaat tctggcgcag aagtgaataa tgctgcccag atccttgagg gaatttttct
      601 tgagcaaggt cgctttgata gggaacaatg ggctctattc ctcgaattgt tggaaaatgg
      661 caaaataaga gtcgtcgata gacttttgga tggctcttgg aaagtcaatg cttgggttaa
      721 gcaagccatt ctttcaggct ttcgagcagg cggcatagct gaatggtctt ggccagcaga
      781 aggtttttac gatagaccag cctttccgcc tcggcatttt gctcgttccg atggtgtgcg
      841 gattgtacct ggaggaagtg cggtgcggag aggcgcttat attgcccaag gagtcgtgat
      901 aatgccgcct tcgtatatca atacaggtgc ctttgtagat caggacacaa tggtggatag
      961 ccatgttttg gttggctctt gtgcgcaaat aggcaagaag gtgcatctat cggcaggggc
     1021 acagatcgga ggggtgctcg aaccaccgca ggcaaatccc gtgattatcg aagacgaagt
     1081 ctttatcggt gggatgtgtg ggatcttcga aggcattctg gtaaagaaaa gagcggttct
     1141 cgctcctggc gtgattatca ccaagggtac aaggatcttc gatttagtta gagagagaga
     1201 actctatggt gaagttccgg agaatgcggt tgtcgtgccc ggcgctcgac ctgctcgcgg
     1261 ttcttatgcg aaggatcggg gcatttcact tgcggcgcct tgtattgtaa aataccgcga
     1321 tgaaaagact gatagcgccg tgatcctgga atcagctttg cgcggctgaa gggaggagtc
     1381 atgcaataca agctcggcat aattggtgct ggtaaaattg gagaggcgat tctcgcgggc
     1441 tctttatcaa aagggctgct gactccttcc gatgtaattc tgtctgtacg aaccgaacag
     1501 cacaggaaga atctagagga tagatataat gtgactgcgg tgcttcgaaa ccgcgaagtg
     1561 gcaaggcagt ctgagatcat tctcattgcg gtcaaaccca ggacaatctt cgacgtactt
     1621 caagaaatcg ctgatatcat ttccgatgaa agtgttgtcg tctcgaccgt tgcgggtgtc
     1681 acgctcgact cgatgcagaa aaagcttgct cgaaaagtgg cactcattcg cactatgcca
     1741 aatcttgggg tgcttgttgg tgaaggcatg accgcactag caccannnnn nnnnnagaag
     1801 aatcaaaaat ccagaaagtc aagcagcttt tcgaatcggt tggccgtgcc ctcgttttag
     1861 atgagcagta tatggatgcg gtgacaggtc tctccggttc tgggccagcc tatgtttacc
     1921 tgataattga agcactgacc gatggtgggg taaagatggg gctgcctcgt gaggtttcca
     1981 tcgagctggc agcacagact gtgcttggtg cggctaagac cgtgcttgtt acaggggaac
     2041 atccggctaa actcaaagat caggttacaa ctcctgctgg gtctactatc gatggcctta
     2101 tggaactcga agatggcgga ctacgggtga cattgatcaa ggccgtggta aaggcaactg
     2161 agcgagctaa taagcttttg cagaagtgaa taccagctca atggttcaag attgttttca
     2221 ggctttgcca ttggttttgt ctctctagag ctattttgcc ttgattatta gcgctttgct
     2281 ctcggcaccg cttagaggga agtctatctt ttattctctt ccaagcaatg aagaaatgag
     2341 atttctcaaa agctccggaa ttttgagggt ctcgccaggc cgaagatttc ttggagcata
     2401 tgatgaaagc aatttctgcg caaaaagctg ggcatcgcgg agcatttttt ctccagtcac
     2461 attatccgta tgctgaatgg ctgtgagtat acgcctctgt cgcccttgcg aaatcaggct
     2521 taagaggcgt tcatgtgcaa tgggctcgat gcatcgcaag agggaagcaa tttcgttatc
     2581 gctcatggaa gagagagttg ccttgagaat tttcgcttcg gcggacataa ttatatcggt
     2641 cgttgtacgg attaggtcag gatctggctg actagattct tcaaaggctt ccattcctgt
     2701 catcaggcct ttatctaaag ccgagctttc ggattcttgc cgagaatata aatcgaggaa
     2761 agcttgctga gcggtacata tttcatcgga aataagtctt ttccgcgctg cagctatcat
     2821 ttctgtgagg atgaatgaac caataggccc aacaagcgcg aggatctgtt caatataata
     2881 gagtgagcat ccagcaaagg cggatgcagc caaaggcata cctgcaattc ggatggtacg
     2941 cttaaacctc aaaggctcgc ttagcgcttc ttcgatgttg tcggtgagat ctccaggcac
     3001 gatatattcg gtaagcttag cattttgaag ctttgacctc tccctgagcc ataggaattc
     3061 cgaattagct gcctcaaggc cggaatatgc tgacataata agaaatacga tataaggatg
     3121 gatgcccctg gcgcatgctt ctgtcccggc caaccacatg agcagctcag gtttattatt
     3181 ttgctgccct ttgaacagga ggccagagcg caaaaaggaa tctcggactg ctgcaggttc
     3241 ggtgtcgctt gcaaggagga gataagacag cttggttgcc tttctcttgg attgttgtga
     3301 tacttcgaac agttccttgc ggttttcgcc cgttttttgg atgatttcca ggaattcgcg
     3361 gactgcttga atctggcgat ttcttctccg atccgactcg atagactgtt taggcgctcc
     3421 ccattggaca tacattttgg cgaggcgttc aatcagcatg ctctgatttc ctttgtgcta
     3481 cggtgtcaag agctgcatct attccacagt atggcaagag tttgcggatc attatttcct
     3541 catcctcgcc atttgctatg gaaagaatag cctcggcggc aacgcattgt ttcagaaatt
     3601 cgataccttc gaccttgctt acgtatagaa gcacggcgag aatctcttct aatatttcac
     3661 ctgatagacc ttcgcttacc agcaggaggc catatcggaa aaagtcatct ttttcggaag
     3721 ataattgctc tgaatcaggc aacatgactc cttgcgcaat cttcttggcc gagtctacca
     3781 ggtaatcgat ataagaaggc aagatcttca tatcttccga actacatgct attttggcgg
     3841 cgagatcggc attccaatag atcattttat ctccgcatat tcctgtttat gccagattat
     3901 agtatgaaat atgggtccga agaaatgggg ctcaaaggca cagctatcaa aacggattag
     3961 gtttcttcct attgcacaaa agcgcatatt atggtatatt ttgtgagcta ttgtgaaatc
     4021 taacaatatt tggtaagatt ttcataattt ttcatagcca atgtagtctc agaatattga
     4081 cgcaatttat agaatgtgat atattcaaat tgaaagcaat aagcgagtgt cggtggcgaa
     4141 acgacattat atagcttttg aaccctttat ggtctgcctc cttccatgaa gggttctttt
     4201 tttttatact agttccatga tactagctga attcgacaat tgggctcgat cttttcttga
     4261 aatcgatcag cttcgacaga tcgatgattc cctcaatggc attcagattg cctgcagtcc
     4321 ttcaaaaccc atacaaaaga tggcggtcgc ggtggatgcc tgcgctgaaa gcattagaag
     4381 agcgcacaat gtgggtgctg atcttttatt tgtccatcat ggtctgtttt gggggaagcc
     4441 agaaccgata ataggcagcc ttagagaaag gctcaaaatt ctaattgatt ccgatatggc
     4501 tctctatgca tgtcatcttc ctctagataa acattccgag gtaggcaaca atgcccgtct
     4561 cgcggatctg cttgagctta aggatagaaa gccttttggc atatatcatg gcattagcat
     4621 tggatggtcg ggtgttctac caaagccagc caaactggat aaaatcatag ctaagattct
     4681 tccggatggg tcggctgcga aatccgtaat tgctgccggg ccgaaagaga tcaatactgt
     4741 tgccattgta tcgggtggag ccccattcga gtctctggaa gccataaggg aaggtatcga
     4801 tctctatata acaggtgagc cttcgcattc tatctaccat tatctcatag aaggtggcat
     4861 aaactttatt gccgctgggc actatgctac agagatctgg ggtgtcaagg ccatagcgga
     4921 aaaagctcac gcagagctgg gtctcgatac tattttcctc gatataccca ccggattgtg
     4981 agatgaagaa aaatgtgcct ctcgttccta ttttgttttc tgggattata attattgtag
     5041 atcagattac aaaggcgctc gttgtaaagt atattccaga aggaagggtt tttgcacgaa
     5101 ttctgggaga ttttgtgtgg atagtgcaca ctaggaacag gggggcagcc ttttccatcg
     5161 gtgctctttc gccgcagccg ctcagatttt tgttttttat ttgcttgcct attgttgtgc
     5221 ttatagcatt gcttttctat tatttcaaat cggaacgcct ttctccgata ctcagatatt
     5281 ctataggtct tatagtgggt ggaggaatag gaaatctgat cgatcgaatt ctacgacccc
     5341 aaggggttgt ggattttatt tcggtcaata tgtatggttt tcttggaatg gagcgatttg
     5401 cgaccttcaa tattgccgat tctgcgatta ctattggcga gatactcttg atacttggat
     5461 ttctgattct agagtttcac aaaccgaagg cacagtcgtc gcagtctgaa aagggatgag
     5521 gatcgatatg aataagaaac aagccaataa aggcaaggtt gctctcttta tagtcggagc
     5581 aaccgtggcg aatattcttc tgatggcgat ttgttttgtg cttttcatgt tgctctattc
     5641 ggttgctttt tctaagtttc ttccgcaaga agccttgata tgggcgatcg gcatagcatt
     5701 tttgctttct cttctcgtat cctcgctcat ctatcgtcgt ttgctgaaat tgttgagaga
     5761 gcgctatcac ctggacgatt atcttggcct aaaggcaaaa tagaaagaca ccgatatcta
     5821 atcgaatgtt ggatgattaa ggcaagcaaa aatgcgcgaa agactaagag attttggtat
     5881 ctcagttcct gagatatatc tgccaagaca aggcatcgat tatcggaaat gggctgtgat
     5941 agcatgcgat caatattctt cagaacgcga atattgggag gatgtggctc gcgaagtaaa
     6001 cgatgctcct tcaaccctta gactgatttt tcccgaatgc tttctcgaag atagtgacat
     6061 cgatcagcga atcttggctg tacataggac aatgcatgag tatttggagc aaggtttaat
     6121 cacctcgagg ggggaagcct ttgtgctggt ggaacggacg acatgcttgc aagaaaagcc
     6181 cagacttgga cttattgtgg caatcgatct ggaacaatat cagtatagtg aggatttaaa
     6241 aacgctgatc cggcccacag aagccacaat taaggaacgt cttccacctc gtatgcggat
     6301 taggcgtggt gctccgctgg aactaccaca cattatgctc ttagttgatg atcccaagaa
     6361 gactcttata gagccgctct atgaacgaag gtattacctc gaaaaagctt atgatttcga
     6421 tttgatgcag aactcaggcc atatccgtgg ttggtttgtg agagaagaca gggagtttga
     6481 cagaattgca caggctctcg aaaacctctc ggatccttgt acattccgtg cgaggtatag
     6541 tcatgacaat ggtgcggatg atgttttgct ctttgcggtt ggcgatggaa atcattcgct
     6601 tgctacggca aaggctattt gggaagagat aaagaatgca tacagagata agcccgacgc
     6661 ccagagaatt ctctcggagc atccagcacg ctatgcgctg gtagagctcg tgaatctcca
     6721 tgatgagggg ctcccatttc atgctattca tcgcgtactc tttaatatcg atgcttcgga
     6781 tttccttcga atgctgagcg ccgtcggcgc agagatacaa tatagatccg atgcaaggca
     6841 ggcatttgcg gaggtcgatt cggagcaaga ttctcacatc tttgcatttg taacggagaa
     6901 taagtcggga acgattcgat ttgataaacc taaaacgctg ctcgcagtct ccaccattca
     6961 agagtacatc gatgaatacc gcaaggttca tccagggttt ggtatcgatt tcatacatgg
     7021 cggcacaagc atagagacta ttggccgcaa aaatggaaat atgggaatct atcttccgcc
     7081 tataaaaaaa gagtcgcttt tcagcattgt gattcatgag ggcttaatgc ctcgaaagac
     7141 attttcgctg ggcgaggctt cggagaagcg cttctatatc gaatctagga tcatttcgcc
     7201 gcaacaataa cataccgctc cgaacctttt cttatacaca aggttcgtag cagtagagaa
     7261 ggtctgcata tatattctat tctagatttc caaagagtgc tattatcctt cgaaaccggg
     7321 aggtgcaaat gctaatactg cttgcagatg cctttacgcc tgatttgccg gagcgacttg
     7381 cggaatttgg cgaggttact tcggatatgt cgcgggttgc tgaggccgag gtgttgatag
     7441 ttcgctccag gacaaaggtc gatggagcca tgctcgatag ggcagctcgt tgtagataca
     7501 ttgtaagagg aggtgttggg cttgatacca ttgatgccga atacgcaaga actaaaggta
     7561 ttatcgtgga taatactccc gaagcctcca gcatcgcggt tgccgaactt gcctttgcgt
     7621 ttatgatcgc aatgcccaat catcttgtac gagccgatat atctatgaaa gaaggaaaat
     7681 ggcttaagaa agaacttgaa aggagcgaac tctatgggaa aacactcggt ctcgtaggca
     7741 atgggcgaat tggtcgggag ttggcgcaca gggcaaaggc ttttggtatg tccgtgattg
     7801 ctttcgataa atatgtaatg agttccgata tagtaagaat tgtctgcttc gatgagcttt
     7861 tggcgctatc ggattatatt agcctacata ttccacttat agacgagaca agaggcatga
     7921 tcgatgcgtc cataatctcc cggatgaaga atggagtcta tcttataaat acatgcagag
     7981 gccaggttgt tgtggagaaa gacatcgccg aagccctcaa atgcggcaaa attgcaggct
     8041 acgcgaccga tgtatattcg aaggaaccac ccgaaggctc tccactcttt ggagcgccta
     8101 atgtgcttat ggcgcctcat attggagcat ctaccgagga aaatcttctg agattagcgg
     8161 attgcatagt tgcaagactc aagaaatatg tgggtagatg agaaaggaat taatttcatg
     8221 aaacgagtga taaatttcaa tgccggacca gctgcgcttc ctatggaagt attgcggaag
     8281 gctcaagagg aaatgctgga ttggcagagt acgggcatgt cagtcatgga agtctctcat
     8341 cgatctagcg aatacgaggc gatgcataat gaatcacagg agctttttcg caaactcgca
     8401 ggtatgggac ccgagtggaa gatcctattc atgaccggtg gagcttcgac gcagttcttt
     8461 atggttccga tgaattacct ctttggcaaa cgtaaggcaa cttatgttgt tactggacac
     8521 tggagcaagg ctgctaagag ggaagcacag cattttggat ctgtcgatgt catcagtacc
     8581 gaaaacaagg atggtagctt tacacgaatt ccaaagcagg aagagctaaa aattgactct
     8641 tcgtcgactt atgtgcacat gactagcaac aacacaatct atggttctca gtggcaatat
     8701 tggccagagt tggagggagt ccctcttgta tgcgatatgt ccagcgatat tttttctcgt
     8761 ccgtttccgg tcgataagct tgccctcatc tatgcgggcg ctcagaagaa tcttggccct
     8821 tcaggtgtca ccgttgtcgc aatcagagag gacttcctgg atattgcacg ggaagggtcc
     8881 aaattgccca caatgctttc ctatcgcact ttttcggaga acaattcgct atataataca
     8941 ccaccatgct tctcgatata tatacttaac cttatgctaa aatggctgct ctatagaatc
     9001 ggcggtctcc aaaaaatgca agaaatcaac gaagagaagg ctcgtattct ctatgaagcc
     9061 atcgatctat cggataactt ctatcgaggg ccggttgaaa agggctcccg ttcacagatg
     9121 aatgttgtat ttagactcaa aacgccggaa atggaagata ccttcatcga gaaggcaagg
     9181 tcggctggaa tagttggagt tagggggcat cgttcgacag gtggaattcg gttttcaata
     9241 tataatgcca atcttgtcga taatgttaga aaagccgcag aattcatgga ggagttccag
     9301 cgtatcaacc gctgaatcct gttagatttc caattcagta ggctggtcta aggattcgga
     9361 ggccagcctt tttatttttg actgatgttt tatatgaaag gataggttat attgaattat
     9421 ggcgaaatcc aggctgcaca ggctatacaa taagtttatc tcttctctca gtttttcggc
     9481 cgagctgcgc aagatgcgcc gtgaactcaa cgtaaagata gatcagcctg aaagtataac
     9541 tgcgcctccc cctttccatc ctcaggcagc taaccgctgg tttaagcgcc gaagaatttc
     9601 catagcagaa tcttatctga tggtggttag agacctcgat tctcgcaact ccagcaggcg
     9661 cttgacagcc ctaaaaaacc tcgccgatgt cgcctttcgt tcatcgagca tcgactatcc
     9721 attgaatacg gcaagggtcc agtcggctct ggtcaaagaa gttgtaaaac accgctcgaa
     9781 taaacggcgt cagttggaat tactctatga tttttcgatg tccacacaag gacaacacca
     9841 ggtgatacgt aagctctgcg atgagttaaa tatcatagag ctgccagaga atggcatgaa
     9901 gattggagaa cttggatatg cctgggatga ccatgtccat gatgttgcaa catccggaag
     9961 aaaaaatccg acccagctcg tactggatgc ctttataaag ggcatatcgt cgttgaccgt
    10021 agcttatggc ctggtttccg atatcgatct gatggaggaa gtgctggaag caggcaatat
    10081 ccttggcctt aaggtaaata ttggactcga gttcagcgtt ctggtggaag gggctcgcta
    10141 ccattttata gccgagcttc ctcatttttc gtcgaaggag gaattgcgct cctttttctc
    10201 ggatcatgca ttagacatgg ataagttctt taagggactc gaaatcaata gagaaagcag
    10261 actcgatgcg gtacgtcgtc tactagaaaa tttcaatcaa aatactctgc caaaaataaa
    10321 tgaaggcttc gagaataaac cagaatactg ccttgcccct ctttcgctgg aagtattgct
    10381 tgcaactatt ccaaacgtga atatcacgcc tcttcatctt gcagaattca tgtatgtgcg
    10441 ctataggcca attctccaga aaagagtgtg gtactataaa gtccttcgag aaaaggcaag
    10501 gtacatagcg caaaatcctt caggccttca gcaagatcaa actaacggtg agttgcaaag
    10561 agaagagatc gaagccagtt attccgaact taaaaaggaa ttgaataggc tttctcctga
    10621 tttggtgctt tcggcctatt tcgattcccc tcatataatt tcctatcagt ccgcttttga
    10681 cgatcttgaa tctataagcc aaatgctaaa aagagcagga tgctccataa ggttcgtcca
    10741 gcctcttgag catgggcttg aactagccaa acaagccctg atcaaatgga gtcgatgcat
    10801 cgacgcggtg gaaatctata acatacagga ttgtatagga agaaatccag aatcaataga
    10861 agactttgcg cgttttgtca acgagctcaa caagagtgcg gcagctgaag gtcgaagctt
    10921 tctaaagcct atctgtggga gtgatgccac agggagaaat ccaaagatcc caggcatggg
    10981 attcatattc gaggatcaga ttatcggaaa tttgcgcaag cgatacataa aacgacatat
    11041 gcgtctccca tcgcttattt ctgctatgat tcgatccagc gaatctccgg tggacgaggc
    11101 aagtctacaa aatgctgctg tcccttctat tgtctgcatg ggcaaggttt cgggtagctg
    11161 gaatcgcgca attacaggcg atgaagagag aataaaccca ttgcgcgctt ggcgatactt
    11221 caacccgaca ttcaagaatg cgattcgtgc gattattggc tttaccgtag ctacagctta
    11281 tataggccct gcttatgccc tgctttggct tggtatcacg ggttttcgaa atagtatcgc
    11341 agacctaatt tcctatagag gtcccaaact cagccagtgg aaacttaaga gcatcaattt
    11401 tgacaatgtc ggtcaatcgc ttttctggac tggcttttct gtacccctta tgggatatgc
    11461 aaaaagcact tttgatatat tgtggccttt agctcctgag acattcttat tcaaccttgt
    11521 aaaattcttt gttatttcga ttgtaaatgg cttctacctt gcggcgcaca acaccttgag
    11581 gggctttgat aagaatgtcg ttagagccaa tctattcaga agtattctgg cctggcctct
    11641 ggctaccata tttgcgccaa tagggaattc tctatctatt ccaagcatcg tgcagaccaa
    11701 gatatggtca gatgttgttg ccggctttat agaaggtgga aataaatatc gcaaggtact
    11761 acaactgcgc cagaagaccc tggaagaact gattcccact gttataaatg ctagcggcaa
    11821 tgcgcaatac attgcgacga tggatgtcct ttacctcttt tctcaagaac cgcgcactag
    11881 aactacgatc aaggcgatct tgagtcccta cgctttcttt actcgtaagc taaaagaaaa
    11941 ttcctcattg cgcttaaata tgctcttgga attcaatgat aaaatgagca aggagagtct
    12001 gtggacagat ctcgtcgatt acatcgtggc aaattacgaa gaggatatgg cagacgattt
    12061 ggtagacctt gttgtggatg gcctacctga gcttttggga gatctaagtg gcctgataga
    12121 aaagtatggg aaagatccaa gccttcttgc aaaactggct gcaaaagcca gtggaacaat
    12181 tcgaggctcg gggcaggcta aataaaatac ctatggctta aagagtccgg aaggcaatta
    12241 ttgctcataa gttccataca tataaatcat cgatagactc taaatcataa acaaaccaat
    12301 aagcaacaaa ttcagagcta taaagtcttg tggtcttttt ttgtgatttt gactcgcggc
    12361 cttatagctc tgagtaatgc cttgcatgca atgcatgctg catggatcgc ttaggaatat
    12421 cgcaaatcag aagggtagtt ctatatcgag tttgcagcgc tccttgactt ctttgaaggc
    12481 cttcttaagg ggcggattga agggcacgcc cttgtctttt ctgtatagcc atgtctcatg
    12541 ctctttttcg ccgggcgtcc atatgcgttg agctcccggg gctttccttg acgctctgag
    12601 ttggcggctt acctctccaa catgatgctt gaaatcctcc aggtcgcaga atgcgctgat
    12661 gttgatggca ataaagctgt gccctaattc gatgggcttg ggcttgccgt ttgagtcgac
    12721 acccagaaga gccttcatga agttggtctg agagagggcg cttgaaagca gctctaccac
    12781 catggcatag ccatagcctt tgtagccgcc aaaatcctca ccgaggccgc caagaggagt
    12841 tagagccgcc ttgtcctgac ctagatctat caagacctgg tgagtatcgt gtctgtattt
    12901 tccatcctgt cctatcaccc atccatccgg aagatccttg ccgagtctgt cgtagagctc
    12961 gattttgccg cgctgtgtca ctgaagtggc gcagtccaac ataaagggaa agtcctcgtc
    13021 cgaaggcatg ccccatgtca tgggattagt gcctagcatg ggctcgactc cccaggtggg
    13081 agcgatggaa ggccgtgcat tagtggtggt catgccaatc ataccatgct caacggcttg
    13141 gcgagcatag tagaaagctg cgccgtagtg tgtcgaattt cgacacaccg tcatgcctat
    13201 accaaattta tccgcttttt ctatggccaa ttccatggac ctttttgcga tatacagacc
    13261 cataccgtta tgaccatcga ttactgccgt agtcggagtc tcccttataa catcgaaggt
    13321 cgttcttgga tctagaattc ccgcccaaat acgatcgaga tagattggtt tatatctacc
    13381 cacgccatga gaatcgacgc cgcgcttatc agcggagatt agtacgtccg agcatacagt
    13441 cgcgacctcg ggagccaccc cgacagcctc gaatccagcc ctcataaagg cgtctacttc
    13501 gtcgaatttc caccatgtga cttcgttgtc cattattctt tctccttatg aatagatttg
    13561 tagttcaagg ttctgtagaa catgatcttt ccaacgatat acatcataag actaaaggcg
    13621 agatattgac aattagcgtt cggaggacct tgaacaagaa ttgaaatgaa aagccgagac
    13681 ataaaatcga gaaagattct tcgtttcaat tctcattatt gatgatacaa ttctattgat
    13741 acaagatata taaacaaacg atagaaaagg aggtcagtat aatgagcgct catccatata
    13801 caataacgct agattttgag aatcttccag ccgagctggt tttcgagcca ggggtcagac
    13861 gagcgccaaa gcgcgacgca gaactaacac ctagccagga acggatcgca ttaaaaaacg
    13921 ccttgcgcta tattccagct cgctggcatg aaagacttgc tccggaattt ctggcggagc
    13981 ttagacagcg aggtcgcatc tatggatatc gttttcggcc tcagggaagg attatcggcc
    14041 ttcccatcga caactattcc ggcaggtgca ttgagggtcg tgctctccga gtaatgatcg
    14101 acaataatct cgacttcgat gtggcactct atccgtacga gctcgtcacc tatggcgaga
    14161 ccggacaggt atgccagaac tggatgcagt atcgacttat ttgtaagtac ctcgaagtac
    14221 tagacgaaaa cagtacgctt gtagtggaat ctggccatcc agtgggcatt tttgctagcc
    14281 atccttttgc gccgcgtgtg atcattacaa atgggctcat ggtgggcatg tacgataatc
    14341 tcaaggactt caatatcgcc gcagcaatgg gtgtagcaaa ctatggccaa atgaccgcag
    14401 gcggctggat gtatattggg ccacagggta ttgtgcatgg cacctataac acgatcctga
    14461 atgcaggcag actcaaactg ggcatctcgc aggataagga tctttctgga agactatttg
    14521 tgtcttctgg gctaggcggt atgtctggag ctcagggcaa ggcggctatg atagctaatg
    14581 cggcctctat catcgctgaa gtggattatt cgcgaatcga aacaagactc aaacagggtt
    14641 gggtttcttg cgaaactgat tcatgcgaag aagctgtcca ccttgctcat gccgccctgg
    14701 acaagggaga gccaattgcg gtcgcatatc atggcaacat tgtcgatctt cttgaatata
    14761 tcgatagcca cgatatacat gtggacctgc tttcagatca gactagctgc catgtcccat
    14821 atgatggcgg ttattgcccg gtcggcataa gcttcgaaga gcgtacaagg cttttggctg
    14881 aggataggca ttatttccgg gctttggtcg atgcttctct gcggcggcac ttcgaagtca
    14941 tatcgcgcct tgtcaaaaat ggcacatatt tcttcgatta cggaaattcg ttcctcaagg
    15001 ctgttttcga tgctggcgta aaagaaatct caagaaatgg gatagatgaa aaagatggat
    15061 tcattttacc gagttatgta gaagatatca tgggccccga gctcttcgac tatggatatg
    15121 ggcccttccg ctgggtatgc ctttctggca aaaaagaaga cctccacaaa accgacctcg
    15181 ccgcgatgtc ctgcatcgat cctgatcgcc gaggccagga tcgcgataac tatatttgga
    15241 tacgcgatgc ggagaaaaac aagcttgttg taggaactca agcacgcatt cttttccagg
    15301 atgcctttgg ccgcatgaat atcgcgctga agttcaataa aatggtgcgc aatggcgaaa
    15361 ttggcccggt tatgataggt cgtgaccatc acgacaccgg cggcgcagac agccccttcc
    15421 gcgagacgag taatatcaag gacggctcga atgtcatggc ggatatggca acccaatgtt
    15481 ttgccggcaa tgcagcaagg ggtatgagcc tcgtaaccct acacaatggc ggcggtgtag
    15541 gcatcggcaa agccataaat ggtggatttg gccttgtgct cgatggctcg gagaaggtcg
    15601 accaggttat acgtatggcc ttaccatggg atgcaatggg cggcgttgcc cggcgctctt
    15661 gggcgcgcaa tgtccatgcc atggaggtag ccgatcagtt caattgcgag tatggcgagt
    15721 atggcactat tacaatgccc catcttgtgg atgag
//
LOCUS       DAOOWY010000039        15656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3768_length_15656_cov_18.414397, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000039 DAOOWY010000000
VERSION     DAOOWY010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15656
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3768_length_15656_cov_18.414397"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PK105_04540"
     CDS             complement(<1..394)
                     /locus_tag="PK105_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61009.1"
                     /translation="MRTSSDNEKANSYLRRGLHELSRHKPAKALGLFRKSIELTPPSC
                     EKRLSRAFYWLSIALLQLNKRDLAVRSLANAQRMNRKGYIRRFYVRHVNGYGMIKQPT
                     KELDDLYAFLSIQLSFYLVKRPNYRFSSE"
     gene            complement(419..2692)
                     /gene="glgX"
                     /locus_tag="PK105_04545"
     CDS             complement(419..2692)
                     /gene="glgX"
                     /locus_tag="PK105_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOU61010.1"
                     /translation="MNDALKSLPRKLTLMPGNPGLLGAIPKAEGVYFSIFSRNARGMI
                     LCLYEKPHDDIPAASLQLDPMRNRTGDVWHCFIPGLKSEVLYLWRAEGPFAPHEGHRF
                     NPYKVLIDPYAKAITDGSLELCSALAYDEESPDKDLSLSTIPNDGSMPKCVVVDDEYN
                     WENDTPLNYPLKNCIIYETHVRGLTKSLTAKVKHHGTYRGVVEMIPYFKSLGITSLEL
                     LPVQEFDSSERFRRNPITGERLSNYWGYSPIAFFAPESSYAFDEGIDHDSYCPSHPVK
                     EFKYMVKELHKAGLEVILDVVFNHTAEGNELGPTLSFRGLDNSIYYMLAEDKRYYRNY
                     SGCGNTLNCNHPVLRTFIMECLHYWVVTMHVDGFRFDLGSILGRDSQGNLLANPPVIE
                     SIAEDPILRDTKIIAEAWDAGGAYQVGSFPGGRWAEWNDRYRDDVRRFWRGDEGFAPL
                     LATRITGSSDLYLNNGRRPFHSINFVTSHDGFTMNDLVSYNYKHNEANGENNRDGIAE
                     NFSFNYGTEGPSNAPFIEETRNRQIKNFFATLLLSIGTPMILGGDEFRRTQNGNNNAY
                     CHDSELNWYDWSLLKKHKDIYRFVKGLIALRKNHDAFRREDFFRGVGKRSIGTFSAAG
                     QSRQNKAKSDRLSSTETPPDILWYDENGQAPKWNKIGKVLVARINEHFSSSELPCSYL
                     LAFNASTEAIQVCLPHDNEHDIWKRVIDTSLPPPHEIEEKEQPVLLDQQKYLMAPRSF
                     ILLISICEPVSDRNLPL"
     gene            complement(2689..3927)
                     /gene="fabF"
                     /locus_tag="PK105_04550"
     CDS             complement(2689..3927)
                     /gene="fabF"
                     /locus_tag="PK105_04550"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU61011.1"
                     /translation="MAKRKVVITGLGLVSPFGNDVPTFWQNVKANRSGVGPITLIDSS
                     DLAVKIAGEVKNFDPSKKMDPKDAKKMDRFAQFAVYAALEALESSNIPREYLDPIRTG
                     VCLGTGQGGTNTIEESIMRLAEKGPNRVPPMTVAKGLANFGAAQIALSLGVHGPNMTV
                     VTACAAATDAIGQAMHLIRDGHADIMFTGGSEASIARMCMASFINIQALSVRNDEPEK
                     ASRPFDRDRDGFVMSEGAGILVLEEYEHAKARGAKIYAEIAGFGSTCDAHHLTAPDPE
                     GLWVSKAIEIALNDAQMKPEDIDYISAHGTSTPLNDPIETKAIKNAFGAHAYKLKISS
                     LKSMIGHCIGAAGALETIAAVLGMNENYVHSTINLDNPDPECDLDYVPHKGINMPVRA
                     FIKNSMGFGGQNAVLVITKP"
     gene            complement(3930..4640)
                     /gene="fabG"
                     /locus_tag="PK105_04555"
     CDS             complement(3930..4640)
                     /gene="fabG"
                     /locus_tag="PK105_04555"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOU61012.1"
                     /translation="MEGTLSGQVCLVTGASRGIGLAIATRFKQEGAIVYGLSRTKAEA
                     DIDWISCDVTDELSVESAIDQIFKIENRIDVLVNNAGMTKDGLIMRMKTEDWEAVLNA
                     NLRSAFFLSRAVSRIMLKQRSGCILNMSSVVGLHGNGGQANYAASKAGLIGFTKSLAK
                     ELGSRNIRVNAIAPGYVETSMTQVLPETAKENLKSSIPLGRPGVPEDIAEAALFLCSS
                     HAAYITGVVLNVDGGMGM"
     gene            complement(4710..5630)
                     /gene="fabD"
                     /locus_tag="PK105_04560"
     CDS             complement(4710..5630)
                     /gene="fabD"
                     /locus_tag="PK105_04560"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOU61013.1"
                     /translation="MKNCFLFPGQGAQYPGMAKDFYDASKAVRELFNEASEAAAMDLK
                     KLIFEGDEETLRQTRNTQISMVVAEAASALMAREHGIMPGGAAGFSVGEWPALAVAKV
                     ISSFDMFRLVSERGRLMEEAGNRAGSTTMSAVLYLSPEKIEQVIHEAGLRQCWIANYN
                     SPVQCVISGTEADIAVAEERLRQAGAKRVIRLKVSGAFHSPLMQDAYESFKELVEATQ
                     FSDPKINLYSNVTGKKMLSKEDAKKYASLQIISPVRWIDEEKSIAADGYDYCFETGPG
                     TVLAGLWKASGSAIPCIAAGTLQDLAAFSL"
     gene            complement(5762..6679)
                     /locus_tag="PK105_04565"
     CDS             complement(5762..6679)
                     /locus_tag="PK105_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61014.1"
                     /translation="MFQLTDAILERIIFAMEDQSKKFLIDLETGDLIDKTALENDDTS
                     KPYLEIGQRFVEPPSWDSHRGFSILEAFAATVTSPASLKIELNAALRRGKGVFRQFRQ
                     VLSKDQELFMRYQEFKLAAMRPIVERWLETLQEGEMLMALKEEPDDLRDIIASDIGFM
                     ISGPDEIAFELESYIDCCIEESFGSLPIAIKRRSLSDLFKEVRTDKNSLFVAFGTIDG
                     TSPLIAGLFLLQTYRGSLMAVVHGVFAAKESAMLDLEWPLIECISDYCARIGATQILL
                     EGPFFSSLLCDNALEHGFFRSGTVFYRVL"
     gene            6748..7668
                     /locus_tag="PK105_04570"
     CDS             6748..7668
                     /locus_tag="PK105_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU61015.1"
                     /translation="MLSNLHLHSRFSDGMSWPEDIALEAARAGLDIVALTDHDTMGGT
                     ERFIQACEARSLKAIQACEIDICEPQIEYKSELLAYFPKADLDSSILYTKRLLQKSLE
                     QRRRRLEFFIESAQELYPDKELSFQDLFRDKTGLDFDEELARKISWSKVDLFLYLKAH
                     RCIKPEMNYKEFKKRFFASGLLKKFKLDKPDVATVVKAVHGDGGFVVIPHLGHFWDDE
                     LPLFQKEAEKLRLLLAWFRRAGVDGVELYWYNSEFKSASFNEQLREIARPQGFFFTYG
                     SDCHGPGSGKHTIMKFKGPFDGFPQKKSIS"
     gene            7665..8237
                     /gene="pth"
                     /locus_tag="PK105_04575"
     CDS             7665..8237
                     /gene="pth"
                     /locus_tag="PK105_04575"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOU61016.1"
                     /translation="MIRLCAFLGNQGQEYRYHRHNVAWLFLDSMRIASELRWSSKFKG
                     AFTQIELGSTIIYFLKPHTFVNLSGESVGEAVHFYKLGLDEILIVHDELEMPFGAFGY
                     KFSGGLGGHNGLRSLEKHLGTRDFWRLRFGIGRPEHDDVAGYVLSPFNPEQLEVLNKK
                     VFPLAERTFYELFYEGIEGFDVRYKKVLPP"
     gene            complement(8295..9170)
                     /gene="era"
                     /locus_tag="PK105_04580"
     CDS             complement(8295..9170)
                     /gene="era"
                     /locus_tag="PK105_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOU61017.1"
                     /translation="MPKSAFVAIIGRPSVGKSTLLNALCGAKVSIVSSVPQTTRNAIR
                     GIITRPEGQLIFVDTPGYHISDKKLNLRLREIVLRSLSEVDLILYLIDATREPGKEEE
                     AIAELLSKDIHRIVVAINKIDDPEARPLLIEEFIIPRLRGAKRVSISAVEKTNLESLI
                     EVLFSLAPEGPFWYPEDIYTDQEPVFRIGELVREKAILNTYQELPHAIAVEYRESSKR
                     ADGVLFARFDILVERDSQKAIVIGRQGSIIKRIREEAEADLKELFDYPIKLQLQVRVD
                     PDWRKSDSALSRIIF"
     gene            complement(9242..10768)
                     /locus_tag="PK105_04585"
     CDS             complement(9242..10768)
                     /locus_tag="PK105_04585"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOU61018.1"
                     /translation="MEKNDRPKICLSMTGKTIEEDLRVLEQYRSVVDCVELRADCLDP
                     SERFFIRDFPEKAGLPCILTIRRLKDGGKFEGGEGTRLVLIARALSFPKPDARANYAY
                     VDLEDDFRAPVVEESCRTFGTRIIRSHHCLGGMPDSLDAAYEELTSEEDEIPRLTVNP
                     QNSADFIRFLEWCLQKPRCDQILIASGDYGIASRILSWRLGSLWTYASPLRGGMDAAA
                     PGHIDPFDLVNIYNFDLVDNDTMIYTLMGQNSIAKSLSPYLHNSAFRKMGKNALLIPT
                     PVDNCEDGLKMLEILGGTGAAITVPFKQDILPYLSFHSTDVQLIGACNTLVLRESGWA
                     GYNTDADGFERSLLEFLGKQDISGCKATIIGAGGAAKSVALSLFRMGARGLVLNRTYS
                     IGRDLARKYNFLYGHLDDRAIDLLLEYSDLIVQATSVGMENEGDPISFYEFKGSEAVF
                     DLVYYPIKTLFLKRAEAAGCRIMNGFKMLCYQAAGQYKLWMGEPPPAIYSQLEQPEQI
                     "
     gene            10928..11620
                     /locus_tag="PK105_04590"
     CDS             10928..11620
                     /locus_tag="PK105_04590"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HOU61019.1"
                     /translation="MPDNSDAITSRFNEKALKAFLDGLLDELRHPQDPKLLEDVRRAF
                     RKKIPLHLRSYAAALMILRAAGLSRAKPQQKMPQQKASPQKSPTPKVPPQPQKKSARN
                     TNWQQGKSSFTAVPSTSASIRSTPSSASSSDSPSSTQELPSKETVSLFVSMGKRHHLK
                     PQELRNEIAEHTGLSLDEIGRIHLLEKYSFVEVPEREAQKVIAAMNGAELNGHVLEIK
                     PAKKRAKTSFRG"
     gene            11627..12319
                     /locus_tag="PK105_04595"
     CDS             11627..12319
                     /locus_tag="PK105_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU61020.1"
                     /translation="MASTIWRLKVGLIGENCYIVESEGKILLIDPGAEAEKIDDFMRY
                     KGIKPSLIILTHGHLDHTAAIPDLFEIWGTELPIAIHPQDAAYLGKDSEQTNRLLFEA
                     IKAPSYFHASWKTMPPPGIFLTDGMAVPGTSLQVLHTPGHSRGSICLYEPELEAGSYG
                     YEEKSMVQGANPSCIISGDTLFRDGVGRTDAPDSDAYALEASLRRLARFSPDTLVFPG
                     HGPRTTIGRELS"
     assembly_gap    12327..12426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12484..13527
                     /locus_tag="PK105_04600"
     CDS             12484..13527
                     /locus_tag="PK105_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOU61021.1"
                     /translation="MESSKFISSHFLVISFVLACTIASRPSFLYAKELSKTDFALGTL
                     CTIRLVEGGNSATLDQAFLRLRTIEDRMSVNKEGTEVSKINEMAGKASVEVSSDTFYV
                     ISKAIDYARLTGGAFDPSIGPLTKLWNIGAGGEKVPPKENILSAKALVDWRNVVLDAS
                     ANKVFLKEEGMSLDLGAIAKGYAADEIEKILRLAKVKAAVIDLGGNIFVYGTKKDKTL
                     WRVGIQNPESERGDYLGIVEGPQMTIVTSGIYERCFIENGKRYHHILDTKTGFPVDNG
                     LVSVSIIGQSSIDADALSTSLIALGIEKGLELLKRFPNTYAIFIDKDKMVYLSPGAEK
                     VFTLLDKNYTLVR"
     gene            complement(13509..14417)
                     /locus_tag="PK105_04605"
     CDS             complement(13509..14417)
                     /locus_tag="PK105_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU61022.1"
                     /translation="MSYVESMHAAEAQIILQIQTFLGLGLRIPMTIVSLFASEAFVIA
                     IVPIVYWCVNRKKGAELGLLVLVSTFINLWLKQIFGWPRPFDIIPSVGLASETSYGMP
                     SGHSQLSVVFAAYIIQFLPKKWRPLMLIGFPLLIGFSRIYLGVHFLTDVLGGYLAGSL
                     FFILYKLGGPSIEKILQKADVRGRVILAAALSFVMNLILPKETMISGAFFGAAIGFTF
                     AARSLAFNEKDRLKIKISRYLVGLITTGIVYVLLKSASGPINNLLGNNQENLLRFLRY
                     ALVGGWVSFGVPFLFLKVGLAHRTSV"
     gene            complement(14519..15448)
                     /gene="speE"
                     /locus_tag="PK105_04610"
     CDS             complement(14519..15448)
                     /gene="speE"
                     /locus_tag="PK105_04610"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOU61023.1"
                     /translation="MRTIIEHNNPHYGFFYEIGSVIVHRKSKWQDIELAESPEFGKVL
                     LLDGITQVGLKDEERYHETLVIPAMIAHPRAREILIIGGGDGCVLREVLACKGVRQVI
                     MADIDAEVISVCREYLPELNKGAFDDPRLRLEIGDGRTFVEHCPDDSFDVVIMDMTDP
                     FGPSERLYTKEFFMQVQRILKGPEGIFAMHGESSIARPIAWACIYKTLNTVFPIVRTA
                     FSFVPMYGTLWSFQFSSCTTDLLTFSNPTMKPTDELKACIAEKRSDEPRFAKAAMWPA
                     LFAEDPSTKAALGLAQSRVITDIHPHFPDVFEP"
BASE COUNT         4218 a         3640 c         3543 g         4155 t
ORIGIN      
        1 cctcagagct aaaacgatag ttcgggcgct tgaccaaata aaaagagagc tggatactta
       61 gaaaagcata tagatcatcg agttcctttg ttggttgctt gatcatgcca tatccattga
      121 catgtcggac atagaacctg cgtatatagc cctttctatt cattctttga gcatttgcaa
      181 ggcttcttac tgcaaggtcg cgcttattca gctgtagaag agcaatagac aaccagtaaa
      241 aggccctaga aagccttttt tcgcaggaag gaggcgtaag ttcgatcgac tttcggaata
      301 atcctagtgc ttttgctggc ttgtgtctgg aaagctcgtg aagacctctt cttagatatg
      361 agttggcttt ttcattatct gatgatgttc tcacgtgtta taagttatta atgaaatttc
      421 ataaaggcaa gttacgatcc gagacaggct cgcatatcga aatcagcaag ataaatgatc
      481 gtggcgccat aagatatttc tgctggtcta atagaacggg ctgctctttc tcttcgattt
      541 catgtggcgg tggcaaagag gtgtctatta ccctcttcca aatatcatgt tcattatcat
      601 gaggaaggca aacctgaatg gcctcagtcg aagcgttgaa agcaagaaga taagaacaag
      661 gcagctctga cgacgagaaa tgttcgttaa tacgcgcgac aagcaccttg cctatcttgt
      721 tccacttcgg agcctggccg ttttcatcat accaaagtat atcgggagga gtttctgtac
      781 tagagagcct gtctgatttg gctttatttt gtctagactg cccagcggcg ctaaatgtac
      841 cgatgcttct ttttcccaca cctctaaaaa aatcctctct tctgaatgca tcgtggtttt
      901 tgcgaagagc gatgagaccc ttgacgaaac gataaatgtc cttatgcttt tttagaagac
      961 tccagtcgta ccaattgagc tcactgtcgt ggcagtaggc attgttgttg ccattctgtg
     1021 ttcgacgaaa ctcatcgcca ccgagaatca tgggcgtgcc tatggaaagc aagagtgttg
     1081 caaagaaatt cttgatctgc cgatttcgtg tttcttcgat aaaaggggcg tttgaaggtc
     1141 cttcggtgcc atagttaaag gagaagtttt ccgctatccc gtcgcggtta ttctctccat
     1201 ttgcctcatt atgcttataa ttataagata caagatcatt catagtaaaa ccatcatgcg
     1261 acgtaacaaa gttaatcgaa tggaatggtc gtctgccatt attgagatag aggtcgctcg
     1321 aaccggtaat tcttgttgct agaagcggag caaatccttc atctcctctc cagaatcgcc
     1381 ttacatcatc acgatatcga tcattccatt ctgcccatct cccaccaggg aaagatccga
     1441 cctggtaagc tccacctgca tcccaggctt cggcaatgat tttcgtgtct cggagaattg
     1501 ggtcttcggc gatagattcg atgacagggg gattggctag cagattaccc tgtgaatccc
     1561 ggcccaaaat cgaacctaga tcgaatcgga acccgtctac atgcatggta acgacccaat
     1621 aatgcaggca ttccattatg aaggtgcgta gtacaggatg attgcaattc agggtattcc
     1681 cacaacccga gtaattgcga taatagcgct tatcctccgc caacatataa tagatcgagt
     1741 tatccagtcc tcggaagctc aaagttggcc ctagctcatt cccttcggca gtatgattga
     1801 acaccacgtc cagaattacc tcgaggcctg ccttgtggag ctcttttacc atgtacttga
     1861 actcctttac aggatgagag gggcaatagc tatcgtggtc aataccctca tcgaaggcat
     1921 aagaactctc cggagcaaag aaagcgatcg gagaataccc ccaatagttc gaaagtctct
     1981 ctccagtaat aggatttcgc ctaaagcgct cggaagagtc gaattcctgt accggaagca
     2041 gctcgaggct cgtaatacca aggctcttga aatatgggat catttcgacc acgcctcgat
     2101 aagtaccatg atgctttact tttgcggtaa gagactttgt caatcctcgt acatgagtct
     2161 catatatgat gcaattctta agtggatagt ttaagggagt gtcgttttcc caattgtatt
     2221 catcatccac aacgacgcac tttggcatag aaccatcgtt tggaattgtc gaaagggaaa
     2281 ggtctttatc cggcgattct tcatcgtagg caagagcgga acagagctcc aaagaaccat
     2341 cggtaatggc tttagcataa gggtctataa ggactttata gggattgaag cgatggcctt
     2401 cgtgtggagc aaatgggcct tccgcgcgcc atagataaag aacctctgac ttaagaccag
     2461 gaataaagca gtgccagaca tctcctgtgc gattgcgcat tgggtctagc tgcagagaag
     2521 ctgcaggaat atcgtcatgg ggcttttcat ataagcagag aatcataccc ctggcgtttc
     2581 tggaaaatat cgagaagtac actccctctg ccttgggaat agcacccaag agtccaggat
     2641 tgcctggcat cagggttagt tttcgaggta gtgacttcaa tgcatcattc atggttttgt
     2701 tatgactaga actgcgtttt gtccgccaaa acccatcgaa ttcttgataa aggctctaac
     2761 cggcatgttt atgcccttgt ggggcacata atccagatcg cattcggggt ctggattatc
     2821 gagatttatg gttgaatgca cataattctc gttcatacca agaacagccg caatagtctc
     2881 gagtgcaccg gcagccccaa tgcagtggcc tatcatggat tttaggctgg aaatctttaa
     2941 cttatatgca tgggcgccga acgcattctt tatggctttt gtttcgatag ggtcgttaag
     3001 aggtgttgaa gttccatggg cactaatata atcgatatcc tcaggcttca tctgtgcatc
     3061 gttaagggca atttcgattg ccttggagac ccaaagaccc tcgggatctg gggctgtgag
     3121 atgatgggca tcgcaggtag aaccaaagcc cgctatctcc gcatatattt tggctccacg
     3181 agctttggca tgttcatatt cctcgagcac caggatgcca gcaccttcgg acattacaaa
     3241 gccatcgcga tctctatcga atgggcgcga cgccttttca ggctcatcgt ttcgtaccga
     3301 aagagcttga atattgataa agctagccat gcacatacga gctatgctag cctccgagcc
     3361 tcctgtaaac attatatctg catggccatc tcggataaga tgcatagcct gcccgattgc
     3421 atcagttgct gcggcgcaag ccgtgactac ggtcatgtta ggcccatgta cacccagaga
     3481 tagagctatc tgtgcagcac caaagtttgc aaggcctttt gcgacagtca ttggagggac
     3541 cctattgggc cctttctctg cgagacgcat aatagattct tctatggtgt tggttccgcc
     3601 ctggccagtg cctaaacaga ctccagtcct tattggatcc aaatattctc taggaatatt
     3661 ggacgactcc aatgcttcaa gggctgcata gacggcaaat tgagcgaatc gatccatttt
     3721 tttggcatct tttgggtcca ttttttttga cggatcgaaa ttcttgactt cgcctgctat
     3781 cttaactgca aggtctgatg aatctatcag cgtaataggc cctacgcctg atctgttagc
     3841 tttgacattc tgccaaaacg taggaacatc atttccaaat gggctaacca ggcctaggcc
     3901 tgtgataacc acttttcttt ttgccataat tacatcccca tgccaccatc aacatttagc
     3961 acaacacctg taatatacgc tgcatggcta ctgcagagaa agagggcagc ctctgcgata
     4021 tcttcgggaa cacccggacg gccgagagga attgaagact ttagattctc cttggctgtt
     4081 tccggtaata cttgagtcat agatgtctcc acatagccgg gcgcgattgc atttacacga
     4141 atattgcgtg atccaagttc ttttgcaagg ctttttgtaa aacctataag accagcttta
     4201 gatgcggcat agttggcttg gcctccgtta ccatgcaggc ctaccacgct tgacatgttg
     4261 aggatacagc ctgaacgctg ctttagcata atgcgtgaga cggcgcggct aagaaaaaaa
     4321 gcgctacgaa gattggcgtt caatacagcc tcccagtctt cggttttcat tctcatgatt
     4381 agaccatctt tggtcatgcc agcattgttt actaaaacat ctatgcgatt ttctattttg
     4441 aaaatttgat cgatagcgga ttccacagat aactcatctg tgacatcgca tgaaatccaa
     4501 tctatatcgg cctctgcttt tgtgcgggat aaaccataga ctatggctcc ttcttgtttg
     4561 aatcgggtag ctatggcaag gcctatgcct ctggaagcac ctgtaacgag acagacctgc
     4621 cctgatagag tgccttccat aatattcact ccttatattt cactatatta tcgagacaac
     4681 taaagtatcg aaccttttgg tcagataatt tatagagaaa aggctgcaag gtcttgcaga
     4741 gtaccagctg cgatacatgg aatagctgag cctgaagctt tccaaagacc tgcaagaacc
     4801 gttcccggtc cggtctcgaa acaatagtca tagccatccg cagctatact cttctcttca
     4861 tctatccagc gtactggcga aatgatttga agcgacgcat atttttttgc atcttctttg
     4921 ctcagcatct tcttgcctgt aacattggag tagaggttga tcttcgggtc tgaaaattga
     4981 gtagcctcta caagctcctt aaacgactcg tatgcatcct gcataagtgg tgaatgaaat
     5041 gcgcccgaaa cctttagtct tatgacacgc ttggctcccg cctggcgcag cctctcttcc
     5101 gctactgcaa tatctgcttc ggttccggaa ataacgcact ggactggact attgtaattt
     5161 gctatccagc attgtctaag gccagcttca tgtataacct gttctatttt ttcgggggag
     5221 aggtataaca ccgcgctcat cgtagtgctg ccagcgcgat tgcctgcctc ttccatcagt
     5281 cttcctcgct ccgagacaag gcgaaacata tcaaagctgc taatgacctt tgccacagca
     5341 agggctggcc attcgccaac agaaaaacca gccgcacctc ctggcattat gccatgctct
     5401 ctggccatga gagcagaagc tgcctcggcc acgaccatgg aaatctgggt gtttctcgtc
     5461 tgcctgagcg tttcttcatc tccttcgaag ataagcttct taagatccat ggcagcagct
     5521 tctgaagctt cgttgaacag ctcgcggaca gctttgcttg catcatagaa atctttggcc
     5581 attccggggt attgcgctcc ctggcctggg aaaagaaagc aattcttcat aagtcctcct
     5641 attagcggca tcttcgatag acattcatca ttgtggttct tagtcctttt tcatgcttat
     5701 atttctcggc catctcttca taagcacata ttttacattc ttaccagatt tgtcattgtt
     5761 ttcaaagaac cctgtagaag acggtgccag atcggaagaa tccatgttca agggcgttat
     5821 cgcacaataa tgaagaaaag aaaggccctt cgagcaaaat ctgtgttgct ccgattctcg
     5881 cacagtaatc agaaatgcat tcgataagag gccattctaa atccagcatt gcactttctt
     5941 ttgctgcaaa tacaccgtgt accacagcca tgagtgagcc cctataggtt tgaagcaaaa
     6001 acagccctgc tattaagggt gaggtaccat cgattgtacc aaatgcgaca aacagcgagt
     6061 ttttatcggt tcttacttcc ttaaataaat ccgaaagcga tcgccttttg atagctatgg
     6121 gcaaggatcc gaaggattcc tctatacagc aatcgatata ggattccaat tcgaaagcaa
     6181 tttcatcggg accggatatc atgaagccga tatcggaagc gattatatct cgcagatcat
     6241 ctggctcttc tttgagagcc attagcattt ctccctcctg aagcgtttcc agccaccttt
     6301 ccacaatggg acgcatagcc gcgagcttga attcctggta tcgcatgaag agttcctggt
     6361 ctttggatag cacctgacgg aattgtctga aaactccctt acctcttcta agtgccgcat
     6421 ttagctctat cttgagacta gccggcgagg taaccgttgc agcaaaggct tcgagaatgg
     6481 aaaagccgcg atgagaatcc catgaaggcg gttccacaaa tcgctggcca atctctagat
     6541 atggtttgga tgtatcatca ttttcaagtg cggttttgtc tatgagatcc cctgtttcaa
     6601 gatctatgag gaattttttc gactgatctt ccatggcgaa gatgattcgc tccagtatgg
     6661 catcggtcag ctgaaacata gtttcattct aatcgatatt ccttcgtcta tggaagggca
     6721 aatttcaata ttgtatacta gggtcaaatg ctttcgaact tgcatctgca ctcgcgcttt
     6781 tcggatggaa tgtcctggcc ggaagatatc gccttggaag cagccagggc tggcctggat
     6841 attgtagctc taaccgacca tgatacgatg ggcggtactg aaaggttcat ccaagcctgc
     6901 gaagccagat cgctgaaggc cattcaggct tgcgaaatag atatatgtga gccacagatc
     6961 gagtacaaga gcgagctttt ggcctatttc ccgaaggctg atctcgattc ttcgattctc
     7021 tataccaaac gcctccttca aaaatcactc gagcagcgca gaagacggtt agaattcttc
     7081 attgaatcgg ctcaagaatt atatccagac aaggagcttt cttttcagga cctttttagg
     7141 gacaagaccg gccttgattt cgatgaagag ctagctcgta agatttcgtg gagcaaggta
     7201 gatttgttct tgtatctgaa ggcgcatcgc tgcatcaaac ccgagatgaa ttacaaagaa
     7261 ttcaagaaga gatttttcgc aagcgggctt cttaaaaagt tcaagcttga caaacccgat
     7321 gttgcaactg tggtcaaggc ggtgcatggg gacggaggtt ttgttgtgat tcctcatctt
     7381 ggtcatttct gggatgatga acttcccctt tttcaaaaag aagccgaaaa attacgactt
     7441 cttcttgcat ggtttagacg cgcaggagtg gatggcgtcg agttgtactg gtacaatagc
     7501 gaattcaaga gcgctagttt caacgaacag ctaagagaaa tagcaagacc acagggattt
     7561 ttcttcacct atggttccga ctgccatggg ccgggttcgg gcaagcatac gatcatgaaa
     7621 ttcaaggggc catttgatgg tttcccacaa aaaaagagca tatcatgata agattatgtg
     7681 cattccttgg gaatcaggga caggaatatc gctatcatag gcataatgtg gcttggcttt
     7741 ttcttgattc gatgcgtata gcctcggaac tgcgctggag ttcaaagttc aagggcgcgt
     7801 ttactcaaat agaattaggt tcaaccatta tttatttttt gaagccacac acctttgtga
     7861 atttatctgg cgagagcgta ggagaagcag tccatttcta taaattagga ttggatgaaa
     7921 tactcattgt ccacgatgag ctcgaaatgc cgtttggcgc ctttggctac aaattctctg
     7981 gcggtcttgg cgggcataat ggattgcgct ctttggagaa gcatctggga accagggact
     8041 tttggaggct tagattcggc attggtagac ccgaacacga tgatgtggcg ggatatgtac
     8101 tatcgccctt caatccagag cagcttgagg ttcttaataa gaaggtattt cctcttgcag
     8161 aaagaacttt ttatgagctc ttttatgagg ggatagaagg attcgatgtc cgatataaga
     8221 aggtcttgcc gccatgaagc gatagagata cgctatcgta atggccagaa ccaaaacatc
     8281 aaaatctcga aatatcaaaa gatgattctc gaaagcgcgg aatcgctctt tctccaatcg
     8341 gggtcgaccc tgacttgaag ctgcaacttt atcggataat cgaaaagctc tttgagatca
     8401 gcctccgctt cttcacgaat gcgttttatt atcgagcctt ggcgtcctat gactatggcc
     8461 ttttgagaat ctctctctac aagaatgtcg aagcgagcaa agagaactcc atcggcacgt
     8521 tttgatgatt cgcgatactc taccgcgata gcatggggta attcctggta tgtattgaga
     8581 atagcctttt ctctgacgag ctctcctatg cggaaaacag gctcctggtc ggtgtaaata
     8641 tcttctggat accaaaacgg tccttcagga gcgagggaaa acagtacctc aatcaaggat
     8701 tcgagattcg tcttttcgac agcggaaatg gaaacgcgct ttgctccgcg gaggcgaggt
     8761 ataatgaact cctcgatcaa taggggcctg gcttcaggat catcgatttt attaatagcg
     8821 acaactattc ggtggatatc tttcgaaaga agctctgcaa tagcttcttc ctccttgcca
     8881 ggttcgcgcg tcgcatctat gagatagaga ataaggtcga cttccgaaag ggatcttaga
     8941 acaatctctc gaagcctcag attgagcttc ttgtccgata tatgatagcc aggagtatcc
     9001 acaaaaataa gttgcccttc aggtcgggtt attatgccac ggatcgcatt tcgtgtcgtt
     9061 tgggggacgg agctgactat agaaaccttt gctccgcata gtgcattcag cagcgtagat
     9121 ttgccgacag aaggcctgcc tatgatcgcg acaaatgcag acttgggcat ttttgcctcc
     9181 tttgcattgt tgaatcatct taaacccttg ctcgcctcga tacctgtata tacttatcga
     9241 tctatatttg ctcaggctgt tcgagttgag agtagattgc gggtggaggt tctcccatcc
     9301 agagcttata ttgaccagct gcctggtaac agagcatttt gaatccattc atgattcggc
     9361 agcctgcagc ttcagcccgc tttagaaata aggtcttgat cggataatag actaaatcga
     9421 aaacagcttc ggaccctttg aactcataaa aacttatagg atcgccctcg ttttccatgc
     9481 ctacactcgt ggcctgtacg atcaagtcgg aatattccaa gaggagatcg atagccctgt
     9541 catcgagatg tccatatagg aagttgtatt ttcgcgccag atcgcgtcct atactatatg
     9601 ttctattgag aacgaggccc ctggcgccca ttctaaacag agaaagagca accgatttgg
     9661 ccgcaccccc tgcccctata attgtcgcct tacatccgga aatatcctgt ttgccgagga
     9721 attccaggag actgcgctcg aagccatcag cgtcggtgtt atagcccgcc catcctgatt
     9781 cgcgtaagac aagagtattg caggctccta tgagttgcac atcggtggag tggaaagaaa
     9841 gatatggcag aatatcttgc ttgaatggta cggtaattgc cgctcctgtg cctccgagga
     9901 tttcgagcat cttaagccca tcttcgcagt tgtctacggg cgtgggaata agaagagcat
     9961 tcttacccat ttttctgaat gcgctgttgt gcaaatatgg cgaaagcgat ttagcgatcg
    10021 agttttgacc catgagcgtg taaatcatcg tatcgttatc aacgaggtcg aaattataga
    10081 tgttgacgag atcgaagggg tcgatatgac ctggcgcagc agcgtccatt ccccctcgca
    10141 aaggactcgc ataagtccat aatgagccta aacgccaaga tagaatgcgc gaggcgatac
    10201 cataatctcc gctagcgatg aggatttgat cacaccttgg tttttgcaga caccactcta
    10261 gaaaacgaat gaaatctgcg ctgttttgag gatttacggt gagcctgggt atttcgtctt
    10321 cttcagaagt cagttcttca taagcagcat cgagactgtc cggcattcca ccgagacagt
    10381 ggtgagatcg aataatacga gtaccaaatg tgcgacagga ttcttccacc actggcgccc
    10441 taaaatcatc ttcgagatca acataggcat aattagctcg agcatcgggc tttggaaagg
    10501 agagtgccct tgctatgaga acaagccgag taccttctcc tccttcaaac ttgccaccat
    10561 ctttcagacg ccttattgtt aagatgcaag ggagaccggc tttctcaggg aagtcacgaa
    10621 taaagaaccg ctcacttgga tcaaggcaat ccgcacgtag ctctacacaa tctacaactg
    10681 aacgatattg ctcaaggaca cgcagatcct cttcgattgt cttgcccgtc atactgaggc
    10741 agatctttgg cctgtcattt ttctccatat ttcctcatcg gttgaaagct tgtataactc
    10801 ttccttattg ttatgcagcg aggctttctt gtctatagtt tctaatacta tagtttacat
    10861 agtggcaaag actgaagtat attagcgcat ataagccatt attccatcaa atagcaggag
    10921 ttgattcgtg ccggacaata gtgatgcaat aacctccaga ttcaacgaaa aagctctaaa
    10981 agcctttctc gatggcctgc tcgatgaact cagacatccg caggacccga aacttctcga
    11041 agacgttcgc agagcattca gaaaaaagat tcccctccat ctccgatctt atgccgcagc
    11101 actcatgatc cttcgtgccg caggcctatc cagggcaaag ccacagcaga agatgccgca
    11161 acaaaaagcg tctccccaaa aaagtccaac tccaaaagta ccgcctcagc cccaaaaaaa
    11221 atcagcccga aacacaaatt ggcaacaggg gaagagctca tttacagcag ttccttcaac
    11281 ttcggcgtcc attaggagca ctccttccag cgcaagctcc tccgattcac catcctcaac
    11341 gcaggagctg ccatcgaagg agacagtatc tctttttgtc tccatgggga aacgacacca
    11401 cttaaaaccc caggagctga gaaatgagat agcagagcac acgggcctat ctctcgatga
    11461 aatcgggcga attcacctgc tcgaaaaata ctccttcgta gaagtgccgg aaagagaagc
    11521 gcaaaaggtt attgcggcaa tgaatggtgc agagctaaat ggccatgttc tcgagataaa
    11581 gccggcaaag aagcgcgcaa agacatcttt cagaggataa taggacgtgg cttcgacaat
    11641 ctggcgtctt aaagtggggc taatcggaga aaattgttat atcgtcgaat ccgagggcaa
    11701 aatactcctc atagatcctg gtgcagaggc cgaaaaaata gatgatttta tgagatataa
    11761 agggattaag ccttctctta tcatattgac acacggccat ctcgatcata cggcagctat
    11821 tcccgatctt ttcgaaatat gggggacgga gcttccaata gctattcatc cgcaagacgc
    11881 cgcttatctt ggaaaagata gcgagcaaac caaccgcctg cttttcgaag caattaaagc
    11941 tccatcctat tttcatgcct cctggaaaac catgccgcct ccggggattt tcttgaccga
    12001 tggcatggcg gtgcctggta ccagtctaca ggttcttcat acaccaggtc acagcagggg
    12061 ttctatttgt ctatatgaac ctgaacttga ggctggttct tatggctatg aagaaaagag
    12121 catggtgcaa ggggctaatc cttcctgcat catctctggg gacactctat ttcgcgatgg
    12181 agtaggacgc acagatgcgc cggattcaga tgcatatgcc ctagaagcta gccttaggag
    12241 acttgcgcgt ttctcgccgg atacgcttgt ctttcctgga catggccctc gaacaaccat
    12301 aggccgagaa ctcagctgag cattatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnncatt tgctgccctg ggaatagttt caaatgaaat tctccatgat aaagccttta
    12481 aagatggaat cttctaaatt catctcgtcg cattttttgg tgattagctt tgtactcgca
    12541 tgcactattg cgtcaagacc ttcttttctc tatgcaaaag agttgagcaa aactgatttt
    12601 gccctcggca cgctctgcac gatccgtctt gtcgaaggag gaaacagcgc tacattagac
    12661 caggcctttc tgcgcttgag aacaatagaa gatcgcatga gtgtgaacaa agaggggaca
    12721 gaggtttcga aaatcaatga aatggcaggg aaggcttcgg tcgaggtttc gagcgatacc
    12781 ttttatgtga tttccaaagc catcgactat gcaaggctca ccggtggcgc attcgaccct
    12841 agcattggcc ctctaacaaa actttggaat atcggcgcgg gcggagaaaa agtccctccc
    12901 aaagaaaaca tcctgtctgc caaagctctg gtcgactggc gcaatgtcgt cctcgatgca
    12961 tccgccaata aggttttctt gaaagaggag ggaatgagcc tggacctggg cgccattgca
    13021 aaaggatatg cagctgatga aatcgaaaag attctgcggc tggcaaaggt caaagcagca
    13081 gtaatcgatc ttggcggcaa tatctttgta tacggaacta agaaagataa aaccctctgg
    13141 cgtgtcggaa tccagaatcc ggaatcagag cgaggagatt accttggcat tgtggaaggc
    13201 ccccagatga ccatcgtaac atccggcatc tatgagcgtt gcttcattga aaatggcaag
    13261 agataccatc acattctaga tacaaagaca ggattccccg tggataatgg cttagtatct
    13321 gtttcgatca ttggccagag cagcatcgat gcggatgcgc tttcgacttc gctaattgct
    13381 ttaggcatcg aaaaaggctt ggaattattg aaacgattcc ccaataccta tgcgatattc
    13441 atcgataaag acaaaatggt atatcttagc ccgggcgcgg agaaagtgtt tacgctgctg
    13501 gataagaatt acacgcttgt acgatgagcc agccccactt ttaagaataa gaaagggact
    13561 ccaaagctaa cccagccacc aacaagcgca tagcgcagaa agcgaaggag gttttcctga
    13621 ttattgccca agaggttatt gataggaccg cttgccgatt ttagcaagac gtacacaatt
    13681 ccagtcgtga tcaaccctac gagatatcgg gaaatcttga tcttgagtct atccttctcg
    13741 ttgaaagcga gactgcgcgc tgcaaatgta aaaccgatag cggcgccaaa gaaagctcct
    13801 gagatcatcg tctctttagg cagaatgaga ttcatcacaa aagaaagtgc cgcggccaat
    13861 ataacacgcc cacgcacgtc tgccttttgg agaatttttt cgattgaagg tccgcccagc
    13921 ttatacagga taaaaaagag acttccagct agatagcctc ctagaacatc ggttaaaaaa
    13981 tgtacaccga ggtatatcct cgaaaatccg ataagaaggg ggaagcctat gagcattaga
    14041 ggtcgccatt ttttgggtaa gaactgtata atataagcgg caaaaactac actcagctgg
    14101 gaatgacccg aaggcatacc atagctagtt tcgcttgcaa gtcctacgct tgggatgata
    14161 tcgaaaggcc ttggccatcc gaaaatctgc ttgagccaga gattgataaa agtagaaacc
    14221 agcaccaaaa ggccaagctc agcccccttc ttgcgattta cacaccagta cacaatagga
    14281 actatcgcga ttacaaaggc ttctgatgcg aataatgata caatcgtcat agggattcga
    14341 agaccaagac ccagaaaagt ctgaatctga aggataatct gcgcttcggc agcatgcatt
    14401 gattctacat acgacataac gctaactata atcgcaataa ggaaaaaaat aaaggataaa
    14461 aatcaaaaaa ggcttctgat tgggcaaaac atactaaatg gaaccgagaa acctagagtc
    14521 agggctcaaa gacatccgga aaatgcggat ggatatctgt tatgacgcgt gactgggcaa
    14581 gaccaagagc tgcctttgtc gaagggtctt ccgcaaatag ggcaggccac atggctgctt
    14641 tagcaaaacg cggttcatcg gaacgctttt ccgcaatgca ggccttcaat tcatccgtag
    14701 gcttcattgt cggattcgag aacgtgagaa gatcagttgt acaggaggaa aactgaaagg
    14761 accacagagt cccatacatt gggacaaaag aaaaagcggt tcgcactatg gggaaaacgg
    14821 tattaagtgt cttatagata catgcccatg ctattggcct tgctattgac gattcaccat
    14881 gcatggcaaa tatgccttcc ggacccttca aaatccgttg aacttgcatg aagaattcct
    14941 tggtgtagag tcgctcagaa ggtccgaaag gatcggtcat atccataatc acaacatcga
    15001 aagaatcatc aggacaatgc tctacaaaag tcctaccatc gcctatttca agcctgagtc
    15061 gaggatcatc aaaggctccc ttattcaact ccggaagata ctctctgcaa acagatataa
    15121 cctctgcatc gatatctgcc attataacct gtcggactcc tttacatgcc aatacctctc
    15181 ttagaacgca accatctcct ccacctataa tgagaatctc tctcgctctt ggatgggcga
    15241 tcatggccgg gatgaccaga gtctcatgat agcgctcctc gtctttgagg cccacttggg
    15301 tgattccatc gagaagcaag acctttccga attctgggct ctctgcaagc tcgatatctt
    15361 gccatttgct cttacgatga acaattacag aaccaatttc gtagaaaaaa ccataatgcg
    15421 gattattatg ctctattata gtacgcatgc aatttcctcc ttttcttgag agtaatatct
    15481 tcttctaaat accgactttt cagattctgc cgctgcgcat ctcgagattt tggcactcga
    15541 atatttcttg aatctcatga attagggcgc gcttaatacg gtttttttct ttggccgata
    15601 agttcttgac ttcatcgcca aaaagaaaat ctttgaggcg gatctctttt ttgcgc
//
LOCUS       DAOOWY010000040        15580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3800_length_15580_cov_6.234332, whole genome shotgun sequence.
ACCESSION   DAOOWY010000040 DAOOWY010000000
VERSION     DAOOWY010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15580
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3800_length_15580_cov_6.234332"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1554
                     /locus_tag="PK105_04615"
     CDS             145..1554
                     /locus_tag="PK105_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61024.1"
                     /translation="MPAKQDIRIEAISLYFLPVKTRIPLKFGIETTTHVCCARVRVIV
                     SSRKGKRAEGWGETPLSAAWAWPSDFSYDARLTAMQDFCVILAQVWNDFKAWGHPMEL
                     GHAFLNDILPEVLASFNEKRMAINLPEMPWLSALLCTASFDIALFDAYGNLHGLPIFE
                     TLNSNFMNKDLAWFYGRKYAQLFKNKYPEDYLVRPAPQTLRAWHLVGGTDILTKDDLT
                     GAEPNDGYPVVLSDWIIRDGLKCLKIKLTGTDAEWDYKRICKVGNIAIQYGVDWLSTD
                     FNCTVTDPRYVCEILDRLMKDAPRIFGMILYIEQPFPYDLDKNRIDAHSVSARKPLFM
                     DESAHDWKYLALGRVLGWTGVALKTCKTFTSALLSLCWAKAMGMTMMVQDLTNPMLAQ
                     IPHLQLAAHAGTIMGVETNAMQFYPDASAIEATVHPGVYRRKNGCVQLDSLGPTGFGY
                     KVKEIGRPLPKAAWEGTQE"
     gene            1551..2339
                     /locus_tag="PK105_04620"
     CDS             1551..2339
                     /locus_tag="PK105_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61025.1"
                     /translation="MSRKRSSLGHTLLNLLYIAIAIAAWQAIVVIFHVKEFVVPSPVA
                     VFKCLFFTEKVAENTWWAHISTTLLEIIVSFAFTAAIGFLLAILICWSKILRSLIMPI
                     IEVLNSLPKIALAPLFLIWLGYGFKANVTIAIICAFFPIVLNTTVGLDSVDEDLLDLV
                     RYLNASKLQIFLKIRIPNSLPFVFSGLKVSATMTVVGTIVGEFVASSKGLGYLIKDSQ
                     AMMNTPPMFAALFLVSAIGLGLFAFIGSLEVKLMPWNRGAEAKS"
     gene            2336..3142
                     /locus_tag="PK105_04625"
     CDS             2336..3142
                     /locus_tag="PK105_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61026.1"
                     /translation="MNKRKSVSFNAARIWEKIKTPAVVLALLILWELCVRIFKIKEYI
                     LPSPIVVLQHLFLKQPDANYNWLIHIRITLTELGISFAITSALGILIAIVLAWSSTLK
                     KIFMPVFVFLNSLPIVAIAPLLVLWFGYGLKTNILIAFLVSFFPIVINTTVGLTEIDE
                     DLLDLVKYLNGTKLQIFLKLRIPNSLPYIFAGLKISSTMCVVGAIVGEFIASDKGLGF
                     IIIASQYTMDSPPIFASLILVSVFGVALFGIVAALERMCMPWYFRVRERG"
     gene            3215..4213
                     /locus_tag="PK105_04630"
     CDS             3215..4213
                     /locus_tag="PK105_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61027.1"
                     /translation="MGKRIFSILAVSFLILAGLTGLSAETTKINFLLNWTISGDHAPY
                     YVAMDKGWFKEEGLDVNVMIGQGSGFSVQMVDAGKAQIGISDAPVPIEARAKGARIKI
                     VGIIFDKHPNCMFFWKDSGIKVPQDIKGKTVAVPASDGHKVMWPAFAKMIGLEPDDVK
                     FINIDPTAKPAALGTRKADVVFELYTGKPFMEKAVPADQLGYILWADYGFNAYAHSYI
                     ANEDFMKNNPEALRKFLKVSYRAWQYTLNNIPEAITILSKYHPINRDDFVANLTAVKE
                     FFKTDRYKNYGIGYIDPARMQDTIDIVKEKGVQMNPDPKYYYTSEFLPSPPYKFDF"
     gene            4333..5100
                     /locus_tag="PK105_04635"
     CDS             4333..5100
                     /locus_tag="PK105_04635"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61028.1"
                     /translation="MSDEQKPIISLKNVKKTYNANGRELEALGDVSFDVFPHEFVSIV
                     GPSGCGKTTLLRIIAGLLPKSSGEIIVDSNNFEITREVGFVFQKPLLLDWRKAIDNVL
                     LPIEVLKLDRKAMMQRAQDLLALVGLEGFEDRYPGELSGGMQQRVSIARALIHDPKML
                     LMDEPFGALDAITRERLNIELLRIWEETRKTILFITHDINEAVFLSDRVIVLSARPSR
                     MVESLDIELPRPRAQDVRAKPKFGEYSLQIYKSLEIG"
     gene            5112..6158
                     /locus_tag="PK105_04640"
     CDS             5112..6158
                     /locus_tag="PK105_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU61029.1"
                     /translation="MSQKCVYGVGMRGAGQVARQHAAAILSNPKLRLVAVSSRSHESA
                     QQFAHECAAASKGMPITIYDRYEDLLDDVNVDIVSECMPNYLHAHEGAQALQAGKHLI
                     LEKPAGISKEELSVLREAATHSQSKTVVSFVLRWHPLVMNLRNLLRKKSIGDVYYAEF
                     DYWHGIKPSFSSYNWIRKREFAGGVMITGGCHVADLARYLHGEAEEVFAYATKKREDF
                     DYPTTIIAAIKFKDGSVGKISASLDGINIPYQLNIDLLGTEGAFRDNKIYSKVLFPYA
                     ENFAVIPCETPNSGSVEHHPFKQEIDNLVDCIDQGIPVLSDVLDACKSMEIALAITES
                     AAIRKPVKVFQEHT"
     gene            6158..6934
                     /locus_tag="PK105_04645"
     CDS             6158..6934
                     /locus_tag="PK105_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOU61030.1"
                     /translation="MEGFQEPIERRIDRMTSATFAEAIKSCGALIIPLGSVEETGNHC
                     PLGTDLFVAESASLRVAEKANCLVSPPIPFGDTHELDFWPGNVHVDAPILGAYIEAVA
                     RSFVRQGFKNIVFFCCHSLTMKCVDLLCRRLNREGVKVCAIDWWKVAVDVARGETSSE
                     EPFGHGGEVITSIALALEPDLVQLTKATDEPSLPALSRINAHSIGSPFIEYADFRKYC
                     ISGAWGAVRATASQEKGERWFLKAISASADFIQSWRENNS"
     gene            6994..8187
                     /locus_tag="PK105_04650"
     CDS             6994..8187
                     /locus_tag="PK105_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61031.1"
                     /translation="MDIIRGRNAITGQPVEVIIEDQNIFKTNICEDKIDLPYISPGFL
                     DMQVNGYYGIDYSLDNLDEDQILSLVECLGKSGTTRHVPTFVSMPNEKLIRNIQTIVK
                     ARKHSPLLKTSIVGLHIEGPFISREEGPRGAHDIQFVRNPDFDLFRKWQEAAEGTIKY
                     ITLAPELDGAIEFIEKAVASGVKAAIGHTAASPEQIRAAIKAGATLSTHLGNGSHTML
                     PRLRNYLWEQLAADELASSIICDGDHLPPAVVRVFLRTKGLERLILVSDGALLGGYPP
                     GIYRWGNLDVQVYNDGHLGIPGTTILAGAAHLLDWDIAKFIEFTGHSLAETIRLCTSN
                     PSRYLNLNEAPENFLKEGEMANFCMFYHRAEQKRLQILRTQLGKHKSYIEGHISDAAG
                     RSSIR"
     gene            complement(8136..8882)
                     /locus_tag="PK105_04655"
     CDS             complement(8136..8882)
                     /locus_tag="PK105_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOU61032.1"
                     /translation="MRICIYKDALDMGKHAAMIAALHIRHAIEKKAECSLILATGTSQ
                     FTMLDALVKEPNIDWSKVEVFHLDEYVGIPDTHNASFRKYLRERFAERVPALQAFHYI
                     HADAPNLSQELTRLSRLIASKTIDVACIGIGENGHLAFNDPPAQLTTKEPYLVVQLDE
                     ACRKQQVGEGWFKDISEVPLQAISMSIPQILKSECIVCSVPDARKAEAVYMAVTAPID
                     PMHPCASLRAHSNCYLMLDRPAASLICPSI"
     gene            9224..10237
                     /locus_tag="PK105_04660"
     CDS             9224..10237
                     /locus_tag="PK105_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOU61033.1"
                     /translation="MKKSLYFVLICMLLFCIGIQGAFAQATWKPTKPINIIVPWGAGG
                     ATDQATRVAAAELEEVLGQKIVIVNQPGASGSVGTKNALEAKRDGYTWTAGAASDLAT
                     YKVLGMLDTDIQKDWEIFLTVANVSVVCVNPNSPYKTFDDLLAAFKAKPGKITVATAG
                     QSSAGHIAIELIRSYTGIEYKHVTYDGGNPAVIACVSGETEVTTQLASEEVDMIRGKK
                     LRPLVVLWDKPLSLEGYGEIPPITNWIKDFQIGLNYFGIFIPKGVPQEVIDTVQKAWR
                     DVIPNSQKLKDYANSRGAIFDPSYGEEAQKRAYSYYQWVAWVYWDAGKAKVKPDTVGI
                     PRP"
     gene            10345..10914
                     /locus_tag="PK105_04665"
     CDS             10345..10914
                     /locus_tag="PK105_04665"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOU61034.1"
                     /translation="MTESTMNKADFYTSIVLLIISLGIIIISIQMPSMTERNESQWSN
                     PGVVPTFIGIALFLLSGSMLLRAIKRGVLKKNENINSDSKNGTISVEEASSISKSPDK
                     NSLFSNPYFRILITIVLCSLYASVLGKIWFPFATFVFIVLFIVVFEFDPKKSLRSQGK
                     KLLWAMVIAACTSAAVTIVFQYLFLVNLP"
     gene            10925..12436
                     /locus_tag="PK105_04670"
     CDS             10925..12436
                     /locus_tag="PK105_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOU61035.1"
                     /translation="MFQGLELFGTAIVSFLNINALLNALWATGLGIIIGMLPGLTATM
                     GVALLTTLTFKMQANDAILVLICMYVGSIYGGSRSAILLNIPGTPANAATTLDGYPLA
                     LQGRAGKAIGIATTGSFLGSMIGMIAMLIFTPVIGNIALQFQSYEFVWFSIFGIIICG
                     NLTAPKDPLKGWIAGFIGLFVAMIGMDGIHAYVRFAFGNTDLAGGISLIPAMVGTFGF
                     AEIISVMRYQRYEVVKTKIERVIPKLSEVFKYWKTIIRSGLIGTLIGAIPGVGEDIAA
                     WVSYDFAKRGARPEEKEKFGKGSVEGLLASETGNNACVPGAIIPVLTLAVPGSAPAAV
                     LLGAMLIHGVRPGPLIMVEFPTFIYECVAMVLIATVMMFILGLTMVRPLVKVLQIPRQ
                     KLMPIVFTLCLIGSYALQSRLFDVTIMVVFGIIGFVLREMEYPMAPLVLGIILGDILD
                     KNLRRALILSNGSFAPFFTRPICLILFALTMLVVLSRAKWVHQLIGSLRKKGS"
     gene            complement(12480..13469)
                     /locus_tag="PK105_04675"
     CDS             complement(12480..13469)
                     /locus_tag="PK105_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOU61036.1"
                     /translation="MIDKHLLGIGKSPQMPYFMLGGFADEAADSIEDQVAATKDLGWK
                     YIEARSVNGKNIHDISNQEFMHVQRILEKNDIRVSCFGSTIANWGASVLQDFSETMET
                     VKRAIERMKRLDVKLIRIMSYAVLTDAEGNPLPDQRVSLRFEYLRAICDEFLAQDMIP
                     VHENCFNYGGLSISHTQELLAAVPGLKLVFDTGNPCLTQDYDKPAPRPYQDAWSTWLA
                     LKTNVVHLHVKDGWYDKATDKEIYVYPGEGPCHVRDILEDCKHSGYGGFLSIEPHMAV
                     VFHDSRVRNSDEMRRSIYREYGRRLEALLQSIGFEIRKGVAYGAMHEPKNELS"
     gene            complement(13477..14523)
                     /locus_tag="PK105_04680"
     CDS             complement(13477..14523)
                     /locus_tag="PK105_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU61037.1"
                     /translation="MNFGIVGTGMIAKMHAKAISEIDGSRLVACFDIIPERARQFAEV
                     YKCRFYNELNKFLDDPEIEVINICTPSGLHSEAAIPAAESGRHIIVEKPLEITVERCL
                     KIVEVAEKNNVVLSGIFPSRFSDASQTVKKACEAGRFGVLSQGNAYVKWWRDQQYYSQ
                     SGWKGTKALDGGGALMNQSIHAVDLLLWFVGPISSLSAYISTVGHNKIEVEDNASAAI
                     EFENGALGAIQGTTAAWPGFLKRLEILGISGSAIMDEDTLSFWKFMEESAEDEKIRKL
                     FSKPASTSGGAADPAAISYLGHKRQLIDVIDSIKKRRKPLVDGIEATKAVACIEAIYR
                     SAELDGAPQNPAKL"
     gene            complement(14535..15377)
                     /locus_tag="PK105_04685"
     CDS             complement(14535..15377)
                     /locus_tag="PK105_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOU61038.1"
                     /translation="MRYAVCNELFGQMPFSKACSLAAQEGFRGVEIAPFSVFENFSQL
                     TIKKKITEIRSILEKENIEFVGFHWLLAKPEGLHIASPNLVIRRRTWDHMRRLLECAS
                     ALGGGKLVLGSPRQRSTPPGMTSDEVKNILREELARIAGFAASCNAMILVEPLSSDQC
                     DVVTSLDEAVNIVKDIDQASIRTMFDFHNAREETKPAHILIEEYWPYIAHIHINEKEG
                     GAPGTGTTNFVPSFRTLLNHGYSGWLSMEIFDVPKKPDLLLHTAISAMRKAEMSALLT
                     GGVD"
BASE COUNT         4235 a         3373 c         3509 g         4463 t
ORIGIN      
        1 cagagtctgc aatagaagaa gcaatcgagt atcgacagtt tggcgatgga gatgcagttt
       61 ggcctttttg aaatatcctg aacttataat ttgagagtca atgcgaaagc ttgactaatc
      121 tcaaggctat ggtatcatca atcgatgcca gcgaaacagg atattcgtat tgaggctatt
      181 tctctttatt ttttgcccgt aaaaacgagg ataccgctaa aatttggcat tgaaactacg
      241 acgcatgtgt gctgcgctcg cgtaagagtc attgtatcat ctcgaaaagg aaaacgagca
      301 gaaggctggg gagaaacccc cttgagcgca gcgtgggcat ggcctagcga tttttcttat
      361 gatgctcgtc tcaccgcgat gcaagacttc tgtgtcattc ttgcacaggt gtggaatgac
      421 ttcaaagcgt ggggacatcc tatggaactc ggccatgcct ttctgaacga tatcctgccg
      481 gaagtgcttg cttcctttaa tgagaagaga atggcaatca atcttcccga aatgccgtgg
      541 ctttcagcgc ttctctgtac ggcttcgttt gatatcgcgc tctttgatgc atatggaaat
      601 cttcatggtc tgccaatctt tgaaacactc aattccaatt tcatgaataa ggatcttgcg
      661 tggttctatg gccgcaagta cgctcaatta ttcaaaaaca aatatcctga agattatcta
      721 gttcgaccag cgcctcaaac cctgagagca tggcacctcg ttggcggtac tgatattttg
      781 actaaagacg atttgacggg agctgagccg aacgatgggt acccggttgt cctctctgac
      841 tggatcattc gcgatggctt gaaatgtctt aaaataaaac taacggggac tgatgcagaa
      901 tgggattata agcggatctg caaagtggga aatatagcga ttcaatatgg tgtggattgg
      961 ctcagcactg acttcaactg taccgtaact gatcctcgct atgtatgcga aattctcgat
     1021 cgtcttatga aagatgctcc tcgcatattt gggatgattc tctatattga gcagcctttt
     1081 ccttatgatc ttgataagaa tcgtatcgat gctcattctg tcagtgcccg caaacctctc
     1141 tttatggatg aaagcgccca cgattggaaa tacctcgctt taggcagagt cctcggttgg
     1201 actggtgtag cactgaagac ctgcaaaact ttcaccagcg ctcttctcag cctctgttgg
     1261 gcaaaagcaa tgggcatgac catgatggtg caggacctta caaatcctat gctagcgcag
     1321 atacctcacc ttcagctcgc tgctcatgcg gggacaatta tgggagtgga gacaaatgca
     1381 atgcagtttt atccagacgc ttctgcaatt gaggctacgg tgcatcctgg tgtgtataga
     1441 agaaaaaatg ggtgcgtgca gttggattct ctgggcccta caggttttgg ttataaagta
     1501 aaagagatag gtcgaccact tcctaaagcc gcatgggaag gaactcaaga atgagcagaa
     1561 agagatcttc tctagggcat acgcttctca atctgcttta catcgcgatt gctattgcag
     1621 catggcaggc aattgtcgtg atctttcatg tcaaagagtt tgttgttcct tcgcctgtcg
     1681 cagtattcaa atgtctcttc tttaccgaaa aagtcgctga gaatacgtgg tgggcacata
     1741 tcagcacaac gcttctggag atcatagtaa gttttgcttt tactgcagcg ataggttttc
     1801 tcctcgcaat ccttatttgc tggtcaaaaa tcctcagaag cctcattatg cctatcattg
     1861 aggtgctcaa ttctttgcca aagattgcac ttgcgcctct tttcctcatt tggctcggct
     1921 atggattcaa agcgaatgtg actattgcaa ttatctgtgc ttttttcccc attgtgctca
     1981 acaccactgt agggcttgat tctgtcgatg aggatcttct tgatttggtc agatatctca
     2041 atgcctcaaa gcttcagatc tttctcaaga tacgaatacc aaattcgctt ccctttgtgt
     2101 tttctgggct taaggtgagt gcgacaatga cggttgttgg cacgattgta ggagaatttg
     2161 ttgcatcgag caaaggattg gggtacttga taaaagattc tcaggcgatg atgaatacgc
     2221 ctccaatgtt tgcggcgctt ttcttagttt ccgcgattgg gttaggtttg tttgcattta
     2281 ttggcagctt ggaagtgaaa cttatgccat ggaatcgagg ggctgaggca aaatcatgaa
     2341 taaaagaaaa agtgtttcct tcaatgccgc tcgcatatgg gaaaagatta agacgccggc
     2401 cgtggtcctt gcattgctta tactctggga attatgcgtt cgtattttta aaataaaaga
     2461 atatatctta ccgtcaccta ttgtcgtgct gcagcatctg ttcttgaaac agccagacgc
     2521 aaactataat tggttgattc atattcgtat cacattgaca gaattaggta tttcattcgc
     2581 aattacctca gcattgggca tccttattgc cattgtccta gcatggagct cgactctgaa
     2641 aaagatcttc atgccggtat ttgtctttct taattcgctt cccatcgtcg caattgcacc
     2701 gcttcttgtt ctttggttcg gctatgggct taaaactaat attcttattg catttcttgt
     2761 atcatttttt ccgattgtca ttaatacaac agtaggcttg acagaaattg atgaagacct
     2821 cttagacctt gtcaaatacc tcaatggaac aaagcttcaa attttcttga agctccgcat
     2881 acccaattct cttccctata tttttgctgg gttaaagatt agctcgacga tgtgcgtcgt
     2941 tggagcaatt gtaggagaat tcattgcttc agataaaggc cttggtttta ttattatcgc
     3001 ctcgcagtac accatggatt ctccgccaat ttttgcaagc ctcattcttg tatcagtctt
     3061 tggtgttgcc ttgtttggaa tagtggctgc actcgaaagg atgtgcatgc cgtggtattt
     3121 ccgagtgaga gagcggggat aaataaattt gtgcatgtgg ggttgtacaa ttaaccctat
     3181 gctactttga ttttgagata aggaggtttc taaaatgggg aaaaggattt tttcaattct
     3241 cgcggtgtcc tttctcatcc tggcaggact cactgggctt tcggcagaaa caacgaaaat
     3301 caacttcttg ttgaattgga caatttctgg cgatcacgct ccctactatg tcgccatgga
     3361 taagggttgg tttaaggagg aagggctcga tgtcaatgtg atgattggtc aaggatctgg
     3421 ctttagtgtt cagatggtcg atgcaggtaa agcacaaatt ggtatctctg atgctccggt
     3481 gcctatagaa gcaagggcaa aaggtgctag aattaagatc gtcggcatca tttttgataa
     3541 acaccctaac tgtatgttct tctggaagga ctcaggaatc aaggttccgc aggatatcaa
     3601 aggcaagact gtggcagtgc ctgcctccga tggccataaa gttatgtggc ctgcatttgc
     3661 gaagatgatt ggccttgagc cagatgatgt gaaatttatc aatatcgacc ctacagcgaa
     3721 accggctgca cttggcacaa gaaaagccga tgtggtattt gagctgtata cagggaaacc
     3781 atttatggaa aaagcagttc ctgctgatca gctaggctat attctctggg ctgactatgg
     3841 tttcaatgcc tatgcgcata gctatatcgc aaacgaagat tttatgaaaa acaatcctga
     3901 agcgctgcgc aaattcctca aggtctccta ccgagcatgg cagtatacac ttaacaacat
     3961 tcccgaagcc atcactattc tttctaaata tcatcctatc aatagggatg actttgtcgc
     4021 aaaccttact gcagtgaagg aattcttcaa gaccgatcgg tataagaact atggcattgg
     4081 ctacatcgat cccgcaagaa tgcaggatac tatcgatatc gtgaaggaaa aaggagttca
     4141 gatgaatccc gatccgaaat attactacac atctgaattt ctcccatctc ctccctacaa
     4201 atttgatttc tgatcgatat caaaaagcat agacgccggg cttgcgatgc agtttctcgt
     4261 ttttgcaact gcccggcaga gcaagatatc gatatatcca aatatccaaa aaagaggaaa
     4321 ggtggatgca tagtgagtga cgaacaaaag cctattattt cactcaagaa cgtgaagaag
     4381 acctataatg cgaacggaag agaacttgaa gcccttggtg atgtaagctt tgatgtcttc
     4441 cctcatgaat ttgtttcgat tgttggacct tctggctgcg gtaagacgac cctcttgagg
     4501 attatcgctg gattacttcc aaaatcctca ggggaaatca tcgtagatag caacaatttt
     4561 gagataacgc gggaagttgg ctttgtattt caaaaacctc tgctcctcga ttggcgaaaa
     4621 gcgatcgata atgtgctgct ccctattgag gttttaaagt tggatcgaaa agcaatgatg
     4681 cagcgggcac aagacctgct ggctcttgtt ggactagagg gatttgagga tcgttaccct
     4741 ggcgaacttt ccggtggtat gcaacagcgc gtttcgatag cacgagcgct catccatgat
     4801 ccaaaaatgc tccttatgga tgagcctttt ggcgcgttgg acgctattac tcgcgaacgg
     4861 ctcaatattg agctcttgcg aatatgggaa gagacaagaa aaacaattct attcatcact
     4921 catgatatca acgaagcagt attcctttcg gacagagtga ttgtactttc agctcggcca
     4981 tcgcgaatgg tagagtcgct cgacatagag ttgccccgac cgcgagcaca ggatgtgaga
     5041 gctaagccga aatttggaga atactcgctt caaatttata aaagccttga aatcggctaa
     5101 ggaagcataa agtgagccag aaatgtgtct atggtgtagg aatgagagga gcagggcaag
     5161 ttgcgaggca gcacgcagca gcaatacttt ccaatccaaa attgcggctc gtggcagtat
     5221 cctcacgttc tcatgagagt gcacaacaat tcgctcatga gtgcgcggct gccagtaagg
     5281 gaatgccaat taccatctac gatcgatatg aggacttact cgatgatgtg aatgtcgaca
     5341 tagtgtcgga gtgcatgcct aattatttgc atgcacacga aggtgctcaa gcacttcagg
     5401 cagggaagca tctcatcctt gaaaaaccgg caggcataag caaggaagag ctctctgtgt
     5461 tgagagaagc agcgacacat tctcagtcta aaaccgtagt gagctttgta ttgcgctggc
     5521 atcctctcgt catgaatctg agaaacctcc tcagaaaaaa atcaattggc gatgtgtact
     5581 atgcggaatt cgattattgg catggcataa agccttcatt ttcgagctat aactggatta
     5641 gaaagaggga atttgcaggc ggggttatga ttacaggtgg ttgccatgtt gcagatcttg
     5701 cgcggtacct tcatggcgag gctgaagaag tctttgcata tgcaacgaaa aaacgcgagg
     5761 attttgatta cccaacaacg attatcgctg cgataaaatt taaagatgga tcggtaggga
     5821 aaatatcagc atcgctggac ggcatcaata tcccatatca actcaatatc gacctattgg
     5881 gaacagaagg ggcttttcga gataataaaa tctattccaa agtcctattc ccttatgccg
     5941 aaaattttgc tgtgatccct tgtgagactc caaattctgg ctccgttgag catcatcctt
     6001 ttaagcaaga gattgacaat ctcgtcgact gcattgatca aggtatacct gtgctatctg
     6061 atgttttaga tgcatgcaaa tcgatggaaa tcgcgcttgc gattactgaa tcggctgcta
     6121 ttagaaaacc agtaaaggtt tttcaggagc atacttaatg gaaggctttc aagagcctat
     6181 agagcggcga attgacagaa tgacatctgc gacatttgcc gaggcaataa aatcttgtgg
     6241 cgcccttatt attcctcttg gttccgtcga agagaccgga aatcactgcc ctctaggcac
     6301 agatctgttc gtcgcggaat cagcgtcttt gcgcgtcgca gaaaaagcta attgcctggt
     6361 ttctccgccg ataccatttg gtgatacgca cgaactcgat ttctggccag gcaatgttca
     6421 tgttgatgca ccaatcctag gcgcatatat tgaggcggtc gctcgatcgt ttgttcgtca
     6481 gggctttaag aatattgtct tcttttgctg tcattcgctc acgatgaaat gtgtggatct
     6541 tctctgccgc cgtcttaatc gcgaaggagt aaaggtgtgt gcgatcgatt ggtggaaagt
     6601 tgccgtcgat gtagcgcgcg gggaaacatc ctcagaagaa ccatttggcc atggaggcga
     6661 agtgattacc tcaatcgccc ttgcgctcga acctgatctt gtccagctta caaaagccac
     6721 cgatgaacct tccttaccag cacttagtcg catcaatgct cactcaatcg gatcgccctt
     6781 tatagagtat gctgatttca gaaaatattg catttcgggc gcttggggag ccgtgagagc
     6841 gaccgcctcg caggaaaaag gcgaacgttg gtttctaaag gctatttctg cttcagcaga
     6901 tttcatacaa tcttggcgtg aaaacaatag ctgatactaa gcccgcgtat tgcatcttgt
     6961 tttgaagagc ctctttcgtg taggaatatt tttatggata tcattagagg cagaaatgct
     7021 atcacagggc aacctgtcga ggttattatc gaggatcaga atattttcaa gacaaacata
     7081 tgcgaagata aaatagattt gccatatata tcgccaggtt tcttggatat gcaagttaat
     7141 ggatactatg gtattgacta tagtcttgat aatcttgatg aagatcaaat tctttctctt
     7201 gtcgagtgcc ttgggaaatc aggaacaact cgccatgtgc ctaccttcgt atcgatgccg
     7261 aatgagaaat taatccgaaa tattcaaacg atcgtcaaag caagaaagca ttctccattg
     7321 cttaaaacat caattgtggg attgcatata gaagggccat tcatttcaag agaagaggga
     7381 cctcgcggtg cccatgatat tcaatttgtc agaaatcctg atttcgatct atttagaaaa
     7441 tggcaagaag ctgctgaagg tactataaaa tatataacat tagcacctga attggatggt
     7501 gcgatcgaat ttatcgaaaa agctgttgcc agtggggtca aagccgcgat cgggcatact
     7561 gcagcgtcgc cagagcagat tagagcggca atcaaagcgg gagctacgtt gtctacgcat
     7621 ttgggcaatg gaagccatac aatgttgccg cgattgcgca actacctttg ggaacagctt
     7681 gctgctgatg aactagcttc cagcatcatc tgtgatggtg accatcttcc tcctgctgtc
     7741 gttcgcgtgt ttcttcgtac taaaggcttg gaaagattga ttcttgtcag tgatggagcc
     7801 ctccttggag ggtatcctcc tggaatatat agatggggta atcttgatgt gcaagtttat
     7861 aatgatggac atctaggtat accaggtaca acgattctcg caggtgctgc acatctattg
     7921 gactgggaca tagcgaaatt tatagagttt accggtcatt cattagctga aacaatcagg
     7981 ctctgcacat cgaatccttc gcgctacctc aatctcaacg aagctccaga gaattttctc
     8041 aaagaaggcg aaatggctaa tttttgcatg ttttaccaca gagcggagca aaagagactt
     8101 cagattctga gaactcaatt aggcaaacac aagtcttata tcgaagggca tatcagcgat
     8161 gctgcaggac gatcaagcat aaggtagcaa ttcgaatgcg ccctaagcga ggcacaagga
     8221 tgcataggat caataggagc agtaactgcc atatatactg cctccgcctt tctcgcatcg
     8281 ggtactgagc acacaataca ctcgcttttg agaatctgag ggatgctcat ggaaatagcc
     8341 tggagtggca cctcagagat atctttgaac catccttccc ccacctgctg tttcctacaa
     8401 gcctcatcga gttgaacaac gagatatggt tcctttgtgg tgagttgagc tggtggatca
     8461 ttgaatgcga gatgtccatt ttctccgata ccgatacatg cgacatcgat agtttttgaa
     8521 gcgatgaggc gagaaagcct tgtaagctct tgagaaagat taggagcatc agcgtgaata
     8581 taatgaaaag cctgcaaggc tggtaccctt tctgcaaagc gctctcgtaa gtatttgcga
     8641 aaactggcat tgtgggtatc gggtatccct acatattcat ctaagtgaaa cacttctact
     8701 ttactccaat cgatgttcgg ttctttcaca agcgcatcga gcattgtgaa ctggcttgtg
     8761 cccgtagcaa gaataagaga acattcggcc tttttctcta ttgcatggcg aatatgcaat
     8821 gcagctatca ttgccgcatg ctttcccatg tctaaggcgt ccttgtatat gcaaatcctc
     8881 atgacgactt tccttttcag cctattttta attataacta actatctttg ctgaaggaat
     8941 agcccatatt gattcattaa aaatctgaca ctataggatt tgtcgcttcg cgacaaatct
     9001 caactgcggt gttgcctttt ggcacaggta catttgattt tctcatgcca gttataagtc
     9061 ttctccgcat gtgcatcatt ttgccatatg aagagtacaa atcctttacc caaatggaga
     9121 atcaaacaat acaaagatat ggatcagtac tttttgggct attgacttat ataaaatggc
     9181 atctataatt tttttagaat tctttaagca ttaggaggct tttatgaaaa agagtctgta
     9241 tttcgtactt atttgcatgt tgctcttttg cataggaata caaggagctt ttgcacaggc
     9301 tacttggaaa ccgacaaagc caataaacat catagtacca tggggtgcag gaggcgcaac
     9361 tgatcaagcg acgagagtcg ccgcagctga gcttgaagag gttctcggcc agaagattgt
     9421 tattgtcaac cagcctggtg cttctggttc tgttggaaca aaaaatgcac ttgaagcaaa
     9481 gcgcgatggt tacacatgga cagcaggtgc tgcatcagac ctcgctacgt ataaagttct
     9541 gggaatgctc gatactgaca ttcaaaagga ttgggaaata ttccttacag tggcgaatgt
     9601 cagcgtagtc tgcgtgaacc caaacagccc ctataagaca tttgatgatc tgcttgcagc
     9661 ctttaaggca aagccaggca agattaccgt agcaactgca gggcagagtt cagcaggcca
     9721 tattgcgatc gagctaattc gatcctatac aggtattgag tataaacatg ttacatatga
     9781 cggtggtaat cctgctgtca ttgcatgcgt atctggagaa accgaggtca ctactcaact
     9841 agcctccgag gaagtagaca tgattcgagg caaaaaactt cgtcccttag tggtgctatg
     9901 ggacaaaccc cttagccttg aaggttatgg agaaattcca ccaattacaa actggatcaa
     9961 ggatttccaa attggtttaa attactttgg aatcttcatt ccaaaaggag ttcctcagga
    10021 ggttatcgat acagttcaaa aagcatggag agatgtgatt cccaattcac agaaactgaa
    10081 agattatgcg aattcccgtg gagccatttt cgatccttca tatggtgagg aggctcaaaa
    10141 gcgagcctat agctattatc aatgggttgc ctgggtgtat tgggacgcag gtaaggcgaa
    10201 agtaaaaccc gacaccgtag gtattcctag gccatagctt acctctctaa aggcaagaaa
    10261 ggcgcaaaca ccgtgcttct ttcttgcctt attttccttg caaataaatc caaaactcta
    10321 tatagagtag aggagatact tataatgacc gaaagtacaa tgaataaagc tgacttctat
    10381 acatcaattg tcttacttat catcagcctc gggatcatta ttatttcaat tcagatgcca
    10441 tcaatgacag aaagaaacga aagtcagtgg tcaaatcctg gagttgtacc aacttttata
    10501 ggcattgcac tatttttgct ctctgggtcg atgttgttgc gcgcaattaa gcgaggggta
    10561 cttaagaaaa acgaaaacat aaattccgat tcaaaaaatg ggactataag cgttgaggag
    10621 gcctcatcaa taagtaaatc tcccgataag aactcgttgt tctcgaatcc atattttaga
    10681 attctcatca caatagtact ctgctcactc tatgcatctg tgctaggcaa aatatggttc
    10741 ccttttgcaa cctttgtatt tatcgtctta ttcatagttg tctttgaatt cgatcctaaa
    10801 aaatcattgc gatctcaagg gaaaaaattg ctatgggcaa tggtgattgc agcttgtaca
    10861 tctgcagcag tcactatcgt attccagtat ctattcctcg taaacttacc ataaggagta
    10921 caagatgttc cagggtctgg aattatttgg tacggcaatt gtttctttct taaatataaa
    10981 tgcgctgtta aatgctcttt gggcaaccgg attaggtatt atcattggga tgctgccggg
    11041 tcttactgca acaatgggtg ttgcgcttct tactaccttg acattcaaga tgcaagcaaa
    11101 cgacgcaata ctcgtgctta tctgtatgta tgtaggctct atttatggcg gaagtcgaag
    11161 cgcaattctc ttgaatatac cgggaactcc tgcaaatgct gcaactacat tggatggata
    11221 tcctttagct ttgcaaggca gagcaggaaa agcaatcggc attgccacta cagggtcctt
    11281 tcttggttct atgatcggca tgatcgcaat gctcatcttt actccggtta ttgggaatat
    11341 tgctcttcaa ttccaatcct atgaattcgt atggttttca atattcggta ttattatatg
    11401 tggcaatctc actgctccaa aagatcctct taaagggtgg attgccggct tcatcggtct
    11461 tttcgtagca atgataggca tggacggaat tcatgcttat gtgagatttg catttggaaa
    11521 cacagaccta gctggcggca tttctttgat ccccgctatg gtaggtacct ttggatttgc
    11581 tgagatcata tctgtcatgc gctatcaacg gtatgaagta gtcaagacaa aaattgagag
    11641 ggtgattccc aaattatctg aagtattcaa gtattggaaa acaataatac ggtctggatt
    11701 aattgggaca ctgattggcg ccattcctgg tgtcggcgag gatattgcgg cgtgggtatc
    11761 gtatgatttt gcaaaaagag gagcaagacc tgaggaaaag gagaagtttg gcaagggatc
    11821 agtcgaggga ctcctcgctt cagaaaccgg aaacaatgct tgcgttccag gagcaattat
    11881 tcctgtgctt actcttgctg tacctggttc agcacccgct gcagttcttt taggcgctat
    11941 gcttattcac ggagtacggc ctgggcctct cattatggtt gaatttccaa ctttcatcta
    12001 tgagtgcgta gcgatggtat taatagctac tgtcatgatg tttattcttg gacttaccat
    12061 ggtgcgacct ctcgtaaaag tgctgcaaat tccaaggcaa aagttgatgc caattgtgtt
    12121 tactctctgt ctaatagggt cctatgcgct tcaatcgagg ctcttcgatg taaccatcat
    12181 ggtcgtattt ggtattatcg gatttgtgct tcgtgagatg gaatatccta tggcacctct
    12241 tgtgctaggt atcattctag gagatattct cgacaaaaat ttacggcgag cacttatcct
    12301 ttctaacggt agttttgcgc cattttttac aaggccgatt tgccttattc tattcgcgct
    12361 tactatgtta gtcgtgttga gcagagcaaa atgggtacat cagcttatag gaagcttgcg
    12421 gaaaaaaggc tcatgaagcc cattctgcac agtgtatgtg ggcaaattca ttatcgcgtt
    12481 taggataatt cattctttgg ctcatgcatt gctccatacg caacaccttt cctaatttca
    12541 aagccaatgc tttgaagcag tgcttcgagc ctcctaccat attctcggta aatggatcgg
    12601 cgcatctcat cgctatttct cacgcgtgaa tcatggaata ccactgccat atgaggctca
    12661 atactaagga agccaccata accagaatgc ttgcaatctt cgagaatatc ccttacatgg
    12721 cacggcccct ctccgggata tacatagatt tctttatcag tagccttatc ataccatcca
    12781 tctttgacat gcagatgtac aacattcgtt ttgagagcta accaagttga ccaagcatcc
    12841 tgatagggtc tgggcgctgg cttgtcataa tcttgagtaa ggcaaggatt tccagtatca
    12901 aatacaagct tgaggccagg aaccgctgcg agcaattcct gggtatgcga tatgctcaaa
    12961 cccccgtaat tgaagcagtt ttcgtgaaca gggatcatat cctgggcaag aaactcatca
    13021 cagattgccc tgagatattc aaaacggaga gatacacgct gatcaggaag gggattgcct
    13081 tcagcgtcgg tcaacactgc atagctcatg atgcgaataa gcttgacatc gaggcgcttc
    13141 atcctttcga ttgcacgctt caccgtttcc atggtctcag aaaaatcttg caaaacgctt
    13201 gcgccccaat tcgctatggt ggatccgaag cagctcacgc gtatatcatt tttttctagt
    13261 atcctctgga catgcataaa ttcctgattg ctgatgtcat gaatattttt gccattcact
    13321 gaacgtgctt cgatatactt ccagcctagg tcttttgttg ccgcgacctg atcttcgatg
    13381 gagtcggcag cttcatcagc aaagcctcca agcataaaat aaggcatctg aggtgattta
    13441 ccaattccca gtaagtgttt atcaatcata tctgcctcat agctttgcgg gattctgcgg
    13501 tgcaccgtca agctccgcac tgcgataaat ggcttcaatg caggcaaccg cctttgtcgc
    13561 ctcaatacca tcgacgagcg gttttcgtct cttttttata gagtctatta cgtcaattag
    13621 ctggcgctta tggccgaggt aactgattgc tgcgggatca gcggcaccgc cagacgtcga
    13681 agcaggctta gagaataatt tgcggatttt ttcatcttca gccgattcct ccatgaattt
    13741 ccagaaacta agagtatcct cgtccataat tgcactgcca ctaatcccca ggatttccag
    13801 acgtttgaga aaacctggcc atgcagcagt agtgccctgg attgcaccga gtgcaccatt
    13861 ttcaaattcg attgctgcgc ttgcattatc ttccacttca attttgttgt ggccaactgt
    13921 tgagatgtag gcactgaggc tactgatagg gccaacaaac cataatagga gatcaactgc
    13981 gtggattgac tggttcatca gggcaccacc tccatccagc gctttggttc ccttccagcc
    14041 gctttgcgaa tagtattgct gatctcgcca ccattttaca taggcattcc cttgagaaag
    14101 cactccaaaa cggccagctt cacaggcttt cttgactgtt tgggaagcat cagaaaatcg
    14161 actcggaaaa ataccagaaa gaactacatt gtttttctcc gcaacttcaa cgattttcag
    14221 gcatcgttct actgtgatct ctagaggctt ttccacaatg atatgcctcc ctgattctgc
    14281 tgcaggaatc gctgcttcgc tatggagtcc cgaaggggtg caaatattga tgacctcaat
    14341 ttctggatca tcgagaaatt tattaagctc attgtaaaat ctgcatttat atacttccgc
    14401 aaactgtcta gcgcgttcag gtatgatatc gaagcatgcg acaagccgcg acccatctat
    14461 ctctgaaatc gctttcgcat gcatttttgc gatcatgcct gtgccaacaa taccaaaatt
    14521 cattgattac ctccttagtc gactcctcca gtcaataatg cggacatttc agcctttctc
    14581 atcgcagaga ttgcagtatg gaggagtaaa tcgggcttct tgggaacatc gaatatttcc
    14641 atgcttagcc aaccactata tccatgattg agcagagtgc gaaagctagg cacgaagtta
    14701 gtggttcccg tacctggagc tccaccttct ttttcattga tatgtatatg agcaatatag
    14761 ggccaatatt cttctataag aatatgcgca ggttttgttt cctctcgagc attatggaaa
    14821 tcgaacattg tgcgaataga tgcttgatcg atatctttta cgatattcac tgcttcgtcg
    14881 agactagtaa cgacatcgca ctggtcagaa gagaggggct cgacgaggat cattgcatta
    14941 catgaagctg caaacccagc aatacgagca agctcttcac gcaaaatatt cttcacctca
    15001 tcagaagtca tgccgggagg agtcgatctt tgccggggag accccaaaac aagctttccc
    15061 cctccaagag cagaagcaca ttcgagcaat ctgcgcatgt gatcccatgt acgccttctg
    15121 atgactaaat tagggcttgc aatatgcaaa ccttctggtt ttgcaagaag ccagtgaaaa
    15181 ccgacaaatt cgatattttc cttttcaaga atactcctga tttctgtaat cttctttttg
    15241 attgttaatt ggctgaaatt ttcaaacact gaaaatggcg caatctcaac acctctgaac
    15301 ccttcttgag ctgcaaggct acatgcttta gaaaaaggca tctggccaaa cagttcatta
    15361 caaaccgcat aacgcataat taattctccg attagaaaat agaagcaccg cggagtgaaa
    15421 atcagtattt cactggtaca cctttcttgt tcgatctgta aattccttca atgatgcgcg
    15481 ccacctggag accatcggag ccgcttacag agggagcttg cccctccaaa atggcgcgag
    15541 caaaatcttc ttcttgctgt gcgaagaaat accatgttgc
//
LOCUS       DAOOWY010000041        15402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3863_length_15402_cov_9.255555, whole genome shotgun sequence.
ACCESSION   DAOOWY010000041 DAOOWY010000000
VERSION     DAOOWY010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3863_length_15402_cov_9.255555"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PK105_04690"
     CDS             <1..206
                     /locus_tag="PK105_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61039.1"
                     /translation="GWKYSSGAIQEALASACGTRIDEKLYVFDYYDSVLEAIGENLEI
                     DFSRQSLTTQEIRHLLAQTKQRT"
     gene            complement(339..1160)
                     /locus_tag="PK105_04695"
                     /pseudo
     CDS             complement(339..1160)
                     /locus_tag="PK105_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679811.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1222..3264)
                     /locus_tag="PK105_04700"
     CDS             complement(1222..3264)
                     /locus_tag="PK105_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU61040.1"
                     /translation="MEEFSLDIENHTLQCFAYNTIIVGSGAAGLCAAERLFSFGQEDV
                     AMVTENLLGGTSRNAGSDKQTYYKLSLGGDSPDSVTELAQTLFKGGCVDGDLALVEAA
                     LSVRAFFHLVELGVPFPRDRYGQYIGYKTDYDPKQRATSAGPYTSKLMTERLEAAIVR
                     KGIKVFDRLQAIKVLTEEKKKVIGLLCLDLDHIDDPDTRYVVFSCTNLIWATGGPACI
                     YSDSVYPISQTGSTGIALEAGVMGKNLTEWQYGLASIRPRWNVSGSYMQALPRFFSTD
                     AFGGNEIEFLDQYFANRNDMLSAIFLKGYQWPFDASKVWGGSSLIDIYVYIERCLKGR
                     QVFLDFRKNASDLSIEITNLIPEAAEYLKKAGGTGKTPLERLTIINKPAIEFYREHGV
                     DLAKEPLEIFMAAQHCNGGLSIDSNWQTNIEGFFAIGEAAGSHGIHRPGGSALNAGQV
                     GALRATEYIAQKRKSSPQKQAPFRNSAEAQISHTITEGEALFLRQKRNENQKNIMQED
                     NGDKENSHIETLRKEASERMTKVGAAFRNLDDIATALTLTHNALTTLNKIATPRSPSE
                     LSAFYHYKDMLLTQLACLASMKDYLEKNGNSRGGALYHSPAGNLPHPTLPDMFKSKKD
                     GDILQDKVQEVTIENLKPHLTWSITWRKVRPIPTVDDIFENIWKEFREENKINKSL"
     gene            complement(3339..4106)
                     /locus_tag="PK105_04705"
     CDS             complement(3339..4106)
                     /locus_tag="PK105_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOU61041.1"
                     /translation="MKKTALITGGCRGIGYAISKQLGLDGYNIVIMASSPVDNYRHAL
                     EELTQAHISYLYVQGDISRSAERANCINKAIAHFGRIDVLVNNAGVAPSQRVDLLDMT
                     EESFDRVVGINTKGTMFMTQAVARQMLTQEFCGPRRGVIINISSISAVVSSTNRGEYC
                     ISKAGVSMLTTLFADRLAAEGIFVYEVRPGVIDTDMTAKVRKKYDELIEQGAFPIKRW
                     GRPEDVASVVSAFCSDKFLYTTGNYIDVDGGFHIQRL"
     gene            complement(4118..5155)
                     /locus_tag="PK105_04710"
     CDS             complement(4118..5155)
                     /locus_tag="PK105_04710"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOU61042.1"
                     /translation="MAEILNTLGIVKEPGVIEFQTKKMGDVPDAKVRIKIISSAICGS
                     DLHIFKGMHPSVQLPVTIGHELSGEIVALGKGCTKFNIGQAVTIEPVITCGKCQACLA
                     GDYGYCENISFTYRNGDGGMANYIDVFEKNVYLLPKGMTYDQGALIEPLSVATHAVRR
                     ASIGLGDKIIIFGAGAIGLFVTALSKKNGAAEVLVVDYSQNRLDFALGLGATYVINPA
                     KESVEEAIAKVSGGTGMDKAFECVGKEETFVQAMTSIRKNGLVTIVGIFENQHITIPA
                     SRFVTHEIHIQGAQGYCRDFPIALHFATELPLERLITHHFALAALQKAFETAFDKSEN
                     PIKIIVHPWAE"
     gene            complement(5148..6503)
                     /locus_tag="PK105_04715"
     CDS             complement(5148..6503)
                     /locus_tag="PK105_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOU61043.1"
                     /translation="MSEFEGFNFKILEDIQRKANEVKADIQFTEDLSPLLQKVKIGPK
                     QAPNSFAILPMEGCDSELDGTPSDLVRRRYRRYASGGAGLIWWEACAVLQEAKANPHQ
                     MSLTSANVGSFASLLHETIKIAQETFGPSHRPINILQLTHSGRYSRPEGKATPLVPQH
                     DPILDPPLGISADSPLISDAYLDDLIGAYVQSALLAQEAGYDGVDIKSCHRYLISELL
                     ASYTRSGRYGGSFENRTRFPLTIIEEIRNAVGSDFIIASRINVFDAHPFPYGWGVDEK
                     DFLKPNLNEPLKYIGLLKKQGINLLSTSAGNPYSPIAYITRPFDQSIIGGKKPHEHPL
                     ESVARLFQFTRMAQAEMKDIPVIGNGYSWLRQFLPYAGAANIANQSCTMVGLGREAFA
                     YPDAPRDIMEKGKMNPRKVCITCSKCTQLMRDHSYTGCVVRDAEIYLKMYNEARKQAN
                     G"
     gene            complement(6559..7410)
                     /locus_tag="PK105_04720"
     CDS             complement(6559..7410)
                     /locus_tag="PK105_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOU61044.1"
                     /translation="MVELIGISLWNWIPIFMEKDISLIEHVASLGFGVVEIGMDSLDF
                     DHKKVGQIAKSLGITIDLCGAFTKGRDLSNFDPSIRESTKKYVIGCLNAAEEMGSKLF
                     AGPVYAGGGKAHLLPLEEKKREWDFAVESLKEITHEAMERGVTIGLEPINRYRTSVIN
                     TVQEALCMVADVDSPALGILFDTYQANIEESNMQEALDTACREHRLVHFHASENHRGP
                     PGSGHIDWNALFSVLATSNYSGHIVMETFAPGGLDAPWTQSIMAPDARASSGLAFLSN
                     LVNRHRL"
     gene            complement(7423..8418)
                     /locus_tag="PK105_04725"
     CDS             complement(7423..8418)
                     /locus_tag="PK105_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU61045.1"
                     /translation="MINLKDVGLRAGVSASTVSRVLRDKGNVNEKTKEKVLDAIKTLK
                     YSPNVLAQGLKLGKTNTIALLVPSIHNEIFPVIARGVENVARKKGFTTILCNTDDDIA
                     IEKEYVAKLKTRYIDGFIVASMLPESDYIRALHEEGFPVVLISRYYEEKIDAVIIDNY
                     QAAYDGVSYLIRTGHRKIAIACGPRELSIYKHRYQGYVDALKAAEIPFDPALVLMETA
                     GNNSFYYLMQNLLKKGLRPDAVFCTSDPKAIIIMRAIRDMRLDIPADISVLGFDNIEM
                     SAFVDPPLSTISQPLYEMGSLGAHKLIDLIGGDGDRREPSVDVLATDLIIRKSTK"
     gene            complement(8424..10085)
                     /gene="ilvD"
                     /locus_tag="PK105_04730"
     CDS             complement(8424..10085)
                     /gene="ilvD"
                     /locus_tag="PK105_04730"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOU61046.1"
                     /translation="MIQEWNEESLDHRNALFYAMGIREEEAKNPVIGIINSWNEMNPG
                     HFHFKHILDAIKGEINAQGGLPRELPVTGICDGICSNTPGDRYTLPARDLVSSEVETI
                     AELNKLDGMVLLGSCDKVVPGMLMGIFRTNIPSVMLTGGYMLPGNHHGKMITLTHTKQ
                     AYAAYVAGTIPHDEYKDIVRHACPTPGICPFMGTAHTMCAIAEILGLTPHGNASVRAE
                     SDEWRQMAAVAARRIMELVHDEIRPLDVVKQPNFENAVAYMMATGGSTNSILHIPAIA
                     RQADIAITPEVFDKISRKVPVISTIYPNHEKYTMVEFSEAGGLGAVMAELARKGLVDV
                     SAKGMFGTLAEKIALSRNYNMEVIHTVDDPILFEGGLAVLSGNIATESAIVKFSAVDR
                     EAWHFRGPARIYESQDEAWQAALKDEIHEGDLVIIRYEGPKGAPGMPHMETFMAAILG
                     KGLGKKIALVSDGRFSGATGGLAIGHVSPEAYEGGNIALLKNGDIIDIDIEARKLMAL
                     VTDEEFAERRRYWKRIEKPSSGWLSLYKKNCSSAHRGASVYWDLG"
     gene            complement(10099..10482)
                     /locus_tag="PK105_04735"
     CDS             complement(10099..10482)
                     /locus_tag="PK105_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61047.1"
                     /translation="MESSNQISEDHFSFEIAHIGINLPNAQEAKKTAALFSSLFHQKV
                     GKESRSSLYAGDFIELMKAPGRGTHGHIAIATNDIEGAKRWFESQGFLFAEDSIKRNQ
                     NSDMTVIYFKDEIAGFAIHLLKRED"
     gene            complement(10496..11194)
                     /locus_tag="PK105_04740"
     CDS             complement(10496..11194)
                     /locus_tag="PK105_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61048.1"
                     /translation="MLHIRNLSASYGHIEALRDVSIDIGKGQIVSIIGSNGAGKTTLL
                     RCISGLIKPTSGSIEFMGKPISQKPHLNVAAGIVHVPEGRKCFSGLTIQDNLLVGGYL
                     IRGKKLLQNLRRAYDLFPILEQRRNQYAGTLSGGEQQMLAICRGLMSDPKLMLLDEPS
                     LGLAPLVMRQVFDLVEQIRNSGITILLVEQNAQKALCACDKAYVLENGRIAICGSGAE
                     LLASPAVKKAYLGE"
     gene            complement(11194..11961)
                     /locus_tag="PK105_04745"
     CDS             complement(11194..11961)
                     /locus_tag="PK105_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61049.1"
                     /translation="MLLQIEKISKSFGGVHALVDFSLSMDRAKIIGIIGPNGAGKTTL
                     FNVISGVYPPDKGKIYLNDKDISGKEQFIITRAGIARTFQNIRLFTGLSVIDNVMCAF
                     DPTSRYGIFGGLFPTPMRIREEKRGHKLSEDYLQLVDLLKYRDEKPENLPYGLQRRLE
                     IARALACKPKILLLDEPAAGLNPKEVSDLMELIHRIRDERGLGILVIEHRLELVMGLS
                     EEIYVLDFGHTIAIGSPSEIKSNPAVIKAYLGEEECN"
     gene            complement(11933..12826)
                     /locus_tag="PK105_04750"
     CDS             complement(11933..12826)
                     /locus_tag="PK105_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61050.1"
                     /translation="MELYLSVFTFALISVVSVCGIYVLTGLTGMFSLGQASFMAIGAY
                     ISGVLVVRLNMPFWPATIIAVLVSIMIGFIVGLPTVRLRRDYISLVTLGFGEAITALL
                     NQSVQITGGAMGFSGIPQKTNFALVIISVIISITLVAFLKISKYGRQCIALKSDELAA
                     KAMGIDVPKMKMIAFLLSVGLTAYSGVLYAFYTTYVDPSMFGWRKSAEWVIMVFFGGV
                     NSLTGSIIATLLLSALPEMLRFASEYRMFFYSVLVLLIINFKPSGLFEEFELSPAFIA
                     KLFKKGNKNGVINATTDRKNQ"
     gene            complement(12838..13710)
                     /locus_tag="PK105_04755"
     CDS             complement(12838..13710)
                     /locus_tag="PK105_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61051.1"
                     /translation="MSLQIIINGIALGSVYALIAVGFALIFNILKFSNFAHGSIMTAS
                     AFLGYLFAIKTKLGLFPVIIVSMLSGGILAILGELIAFRRLVVRKASPIYYFVSSITL
                     GSLYEAIVTIGAGPNFYNYPRFFKKLVISIGNIVISRSDMIMFLTSWLALGLLVVMLA
                     KTRIGRAVRAVSFDRGTASLMGIDTIHHIQLAFFLSGALGGLSGVFLGINYTLYPQLG
                     NLVTKGFIASVIGGLGSMSGAVIGAVLLGLTETLLINFIGSGYAPVFTFLIMLVFLLV
                     HPQGISGSNVQEKA"
     gene            complement(13800..14948)
                     /locus_tag="PK105_04760"
     CDS             complement(13800..14948)
                     /locus_tag="PK105_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61052.1"
                     /translation="MKKTIAILLLALAVAVSANAAPASNEVLVGCLQDITGPTSTLGK
                     MIKEGAQWAVDEINKSGGIKGRQVKLIVYDTRGDVQEAINAFKRLCTSDKVSAIIGPP
                     VANIGIAIAPISEQYKVPVLGFAIDDRATIKPSGQPYKNMFLFQPSSDQQGAIMADFA
                     VRERNLKNFGIIYNQGNAYSVSLVGPFKDTLKNLPGTNVAIEVPYQPNDKDFKTMLSK
                     IKAANVDAIYAPNYTQELILISQQARAIGFNGPLIFGLDACPPFASLCGPEADGVIYI
                     NNITESDPQIQDISQAYLQKTGNIATNKFFLGYDVMKIIAKIVETVGDDPEAVQKAVE
                     NLTGYVGLTGTITINPATHQTIGLEMIMHEIKNGQPVVLKRYTAPTKK"
BASE COUNT         4021 a         3609 c         3389 g         4383 t
ORIGIN      
        1 ttgggtggaa gtattcctca ggggcgattc aagaagcatt agcgagtgcc tgtggcacaa
       61 ggatagatga gaagctctat gtgtttgact attatgattc ggtccttgag gcgattgggg
      121 aaaaccttga gattgatttt tctcgccagt cgctcactac ccaggagatt cgccatcttt
      181 tagcccagac aaagcaaaga acctaggaga tggactacac tctatatcgt aaagacattc
      241 tcactattca ttatagcatc attgattata agctattttt aggaaataaa gtggaaaagt
      301 cagattgtca agactcgatt aaaatgtcgc ctttccattc aggctgagcg agtggccagt
      361 tcctccttgt gtggtagttg ctcgagttcg acatactcga gttcagtgtt ctggtagagc
      421 agcgtgattc gcccatcgag cagttctctg accgttagct gtgcccgtgg cttggggagg
      481 actcgtgcag tgcgcttgat ctgtaccagc cggcgtttgt agctgacgac atagtcattg
      541 ctcaccacac gaggcgtttc atagcacacg atgtcctcaa gcgaggttgg tgtcgtgagc
      601 ggcacatgag catcctgtgg ctgtgcaggt gccttcgcaa acttctcgtt gatcttcgga
      661 agggagactt ccttgagata ccgagtggca tcctcaatcg tcgagatgcc ggccagcctg
      721 agttctttca ccagccggtg ctgatacacc ccatggcttc gttcaacccg ccctttcatc
      781 ggacgtggtt tcttcttcac agaaaaatga gaagatgatt cctgtcgcgt catccaccat
      841 attcatcagg caccaggcac tccttcgccc ttcaaaccaa tcgtgatggc tcccgtcaaa
      901 ctgcaagagc tccctaaacc attcccaccg cctttcgcat ccccgcatca taggccctcc
      961 ctgaggcctt cccttggtta cggtgcagca ccccttcgtc gccttcctcc agatatcgcc
     1021 ggtagatccg tttcgattgc cgatacgaca ctttaagctt tactgccgcc tcttttcagt
     1081 gaaagctcct tttgtactac ctgttccatc acttttgccc gtaccagctc tttttgtccc
     1141 atcgctaatt tccccgccat cttcgctcct ggctgttatg ctactaaggg gacattatca
     1201 tggagtttca acataaaaat tttaaagaga tttatttatc ttattttctt cgcgaaattc
     1261 cttccaaata ttttcaaaaa tgtcatctac agttgggata gggcgcacct ttctccaggt
     1321 aatgctccat gtgaggtgtg gcttgagatt ctctatggtg acttcttgta ctttatcttg
     1381 caaaatatct ccatctttct ttgacttgaa catgtcagga agcgtgggat gaggaaggtt
     1441 tcctgcagga ctgtgataga gggctccgcc tctgctattg ccattctttt ctagatagtc
     1501 tttcatcgaa gcaaggcagg caagctgggt aaggagcata tctttgtaat ggtagaatgc
     1561 tgatagttca gatggcgatc gaggagtagc tatcttgttt agtgtggtga gtgcgttatg
     1621 agtgagagtc aatgctgttg caatatcatc taaattcctg aatgcagctc ctaccttggt
     1681 cattctttct gaagcttcct tgcgcaaggt ttctatatgc gaattttctt tatcaccatt
     1741 atcttcttgc ataatgtttt tttgattttc gtttcttttt tgacggagaa ataaagcttc
     1801 gccttctgtt atcgtgtggg atatctgggc ttcggcagaa ttgcgaaaag gtgcctgctt
     1861 ttgaggggat gatttgcgct tttgtgcaat gtattcggtg gcgcgcaaag cgcctacttg
     1921 gccagcattg agagcagagc ctccaggtcg atgaatacca tggcttcctg cagcctcacc
     1981 gatagcaaag aatccttcga tgttggtttg ccaatttgag tctattgata atccaccatt
     2041 gcaatgttgt gcagccataa aaatttcaag gggttctttt gcaagatcaa caccatgttc
     2101 tctatagaat tcgatggcag gtttattgat gatggtcagc ctttcgagtg gcgtcttgcc
     2161 cgttcctcct gcttttttga gatattctgc tgcttcggga atgaggttcg ttatttcaat
     2221 agacagatca ctcgcatttt tacgaaaatc caaaaatact tggcggcctt tcagacatcg
     2281 ttcgatataa acatagatat cgatcaatga agaaccgccc catacttttg aagcgtcgaa
     2341 aggccactgg tagcctttga gaaaaatagc cgaaagcatg tcattgcgat tagcaaaata
     2401 ttggtcgagg aattctatct cattgccccc aaatgcatcg gtagaaaaaa agcgcggaag
     2461 tgcctgcata taggaaccag aaacattcca gcgtggcctt attgaagcaa gcccatattg
     2521 ccactcggtg agattcttgc ccataactcc ggcttctaag gcaataccag tagaacccgt
     2581 ctggcttatg ggataaacag aatccgaata gatacatgct ggtcctcccg tagcccatat
     2641 gagatttgtg caggaaaaaa cgacatagcg cgtatcagga tcgtcgatgt ggtccaaatc
     2701 gagacataag agcccaatta ctttcttttt ttcttctgtc aatactttaa tcgcttgaag
     2761 cctatcgaaa actttgatcc cttttcgcac tattgccgct tcgagtcgct cggtcatgag
     2821 ctttgaggta tagggtcctg ctgaagtagc tcgttgtttg gggtcataat ccgttttgta
     2881 gccaatgtat tggccatatc gatcacgagg aaagggaact cccaattcga caaggtgaaa
     2941 aaaggctctg actgaaaggg cagcttctac taatgcaaga tcgccatcga cgcatccccc
     3001 cttaaaaagc gtttgtgcaa gctcagttac tgaatcaggg ctatctcctc ctaagctaag
     3061 cttataataa gtctgtttat ctgatcctgc attgcgcgaa gtacctccca ataggttttc
     3121 tgtcaccatt gcgacgtctt cttgaccaaa agagaaaagc cgctcggctg cgcaaaggcc
     3181 cgctgcccct gatcctacga tgatggtgtt atatgcgaaa cattggagag tatgattctc
     3241 gatatcaaga gaaaattctt ccattgtggc cccctaaact ttcaagataa tcgattcaga
     3301 actttgaaga tgatcgcccc ctaaccccaa gctcttacct aaagtctctg aatatggaat
     3361 cctccatcga catcgatata attgccagtg gtatagagga atttatccga gcagaaagca
     3421 gagaccacgc ttgcaacatc ttcagggcgt ccccagcgct ttatggggaa agctccctgc
     3481 tcaatgagct catcatattt ttttcttact tttgcggtca tatcggtatc gattacccct
     3541 ggcctcactt catagacaaa gattccttca gcagcgagcc tgtcggcaaa gagcgtagtg
     3601 agcatggaaa cgccagcttt ggatatgcaa tattctcctc gattggtact cgaaaccacc
     3661 gctgatatgg atgaaatatt gatgatgaca ccccgtcgag gaccacagaa ttcctgtgtt
     3721 agcatttggc gcgcaacggc ctgggtcatg aacattgtcc ctttcgtgtt gatcccgact
     3781 actcgatcaa agctctcttc ggtcatatcg aggagatcga ctctttgaga aggagcaacg
     3841 ccagcattat taacgagcac atcgattcgt ccaaagtggg ctattgcctt gttgatgcaa
     3901 tttgctctct ctgcgctgcg cgatatatct ccctgtacat aaaggtagct gatgtgtgcc
     3961 tgagttagtt cctccagcgc gtggcgatag ttatcgacag gcgaacttgc cattatgacg
     4021 atattatagc catccaaacc aagctgtttt gaaatagcat aaccaattcc gcgacatcca
     4081 ccggtaatta aagctgtctt tttcatcgaa attctcgcta ttctgcccag gggtgcacaa
     4141 taatttttat tgggttttcg gacttatcga atgcggtctc aaaagctttc tgtaaggcgg
     4201 caagcgcaaa gtgatgcgtg atgagtcttt ctagtggcag ctccgtggca aaatgaagcg
     4261 caataggaaa atctcggcaa tatccttgtg caccttggat atgtatttca tgcgtgacaa
     4321 aacgactcgc ggggattgta atgtgctggt tttcaaaaat gccgacgata gtcaccaatc
     4381 cattcttgcg aatgcttgtc atagcctgga caaaggtctc ttctttgcct acacattcga
     4441 aagccttgtc cattcctgta ccccctgaaa cttttgctat cgcctcctct acagattctt
     4501 ttgcgggatt gatgacatag gtggcaccaa gaccaagtgc gaaatccagc ctattttgag
     4561 aatagtccac cacgagcacc tcagctgccc catttttttt gctgagtgcg gtgacgaaaa
     4621 ggccgattgc tcctgcgcca aaaataatga ttttatcacc aaggcctatc gaagctctgc
     4681 ggactgcatg cgtggctacc gaaagaggct cgataagcgc accttgatcg taggtcatcc
     4741 cttttgggag taagtacacg ttcttttcaa atacatcgat gtaatttgcc atacctccgt
     4801 cgccattccg ataggtgaaa cttatatttt cacaatatcc atagtctcca gcaaggcaag
     4861 cctggcattt tccacaggta attacgggct cgatggtgac tgcctgacca atattgaatt
     4921 tggtgcagcc ttttcccaaa gctactatct ctcccgaaag ttcatgtcca atagtgacag
     4981 gcaactgtac cgaagggtgc atgcctttga agatgtggag atcgctccca catatggcac
     5041 tcgaaatgat tttgattctg acttttgcgt ctggaacgtc tcccatcttt tttgtttgga
     5101 attctatgac acccggctct tttactatgc ctaatgtgtt caatatctca gccatttgct
     5161 tgtttccttg cttcattgta catcttcaaa tagatctccg cgtctcgaac gacgcagccc
     5221 gtataactat gatctctcat caattgggta cacttgctgc aggtgatgca cacttttctg
     5281 gggttcattt tgcctttttc cataatatcg cgtggtgcat ctggataggc aaacgcttct
     5341 cttccaaggc ctaccatcgt gcacgattga ttcgcaatat ttgctgctcc cgcataaggg
     5401 aggaattgac gaagccaact atagccattg cctattacag gaatatcctt catttcggct
     5461 tgagccatgc gagtgaattg aaagagcctc gcgacgcttt cgagcgggtg ctcatgaggc
     5521 tttttgcctc ctatgatcga ctggtcaaaa ggacgagtaa tgtaggcgat aggagaatag
     5581 ggattacccg cggaagtcga aagaagattg ataccttgtt tttttaatag tccaatatat
     5641 ttcagcggct cattgagatt gggtttcagg aaatcttttt catcgacacc ccatccataa
     5701 ggaaaaggat gtgcatcgaa cacattgatc ctggaggcaa tgataaaatc actccctact
     5761 gcattgcgaa tttcctcgat tattgtaaga ggaaatcgtg ttctgttttc aaaacttcct
     5821 ccatatctgc ccgatcttgt ataggatgca agaagttcgg agataagata acgatggcag
     5881 cttttgatat ccactccatc gtagcctgct tcttgtgcaa gcaaggctga ttgcacataa
     5941 gctccgatga gatcgtcgag atatgcatca gagatgagcg gcgagtcagc tgaaatgcct
     6001 aatggtggat cgaggatcgg gtcgtgctgt ggcacaagcg gtgtggcttt cccttctggc
     6061 cgcgaatagc gacccgaatg agtaagctgc aagatattga ttggtcgatg cgatggaccg
     6121 aaagtctctt gggctatttt gattgtctca tgcaagagtg aggcaaagga accaacattt
     6181 gccgaagtta aactcatttg atgtggattg gctttcgctt cttgcagcac cgcgcaggct
     6241 tcccaccaaa tgagtccagc tcctccagat gcataacgtc gatatctgcg tcgaacgaga
     6301 tcagaaggag ttccatcgag ctcgctatcg catccttcca taggaaggat agcgaaggaa
     6361 ttgggggctt gctttggacc gattttgact ttttgaagga gaggtgacaa atcttctgta
     6421 aattgaatgt cagcctttac ttcgttcgct tttctctgta tgtcctcaag aattttgaaa
     6481 ttgaatccct cgaattctga cattattttc tcctgattcc cttctatatc tatattttta
     6541 tatcaatgat cgtgtatttc ataatctgtg gcgattcaca agatttgaga gaaacgccaa
     6601 tcctgaggat gctctggcgt ctggagccat gatcgattga gtccaaggag catcgaggcc
     6661 accaggagcg aaagtttcca taactatatg accgctgtag ttcgaagtag caagcacaga
     6721 aaagagtgcg ttccaatcta tatggccgct tcctggtgga cctctatggt tctcgcttgc
     6781 atgaaaatgt accaatcgat gttctctaca tgctgtatcc aaggcttctt gcatattgct
     6841 ttcctctata ttcgcctgat aggtatcgaa taagattcct aaggcgggac tgtccacatc
     6901 tgcgaccatg cagagagctt cttgcactgt attgatcacg ctcgtgcggt agcggttgat
     6961 aggctcaagt ccaatggtga cccctctttc catagcttca tgcgttattt ctttcaagct
     7021 ctccacggcg aaatcccatt cgcgcttttt ttcttctagt ggtaataaat gcgccttgcc
     7081 gccgccagca tagactggac ctgcaaaaag ctttgagccc atttcctcag ccgcattgag
     7141 gcatccaata acatactttt tggtggattc tcttatagag gggtcgaagt tcgacagatc
     7201 tcgacccttg gtaaaagcac cacagagatc aatcgtgatt ccaagcgact ttgcgatttg
     7261 ccccactttc ttatgatcaa agtcaagcga gtccatgcct atttcaacta caccaaaacc
     7321 gagcgaagcg acatgctcga tgagggatat atctttttcc atgaaaatgg gaatccagtt
     7381 ccagaggctt ataccaatga gctctaccat ggttttatac ctctacttgg tggatttgcg
     7441 aattattaga tcagtggcca gaacatccac cgatggctct ctcctatctc catctcctcc
     7501 gatgagatct atcaatttgt gggcacccaa tgaccccatt tcatagagag gctgggatat
     7561 cgtagataga ggaggatcga cgaaagcgct catctcaata ttgtcaaagc ccagtacaga
     7621 gatatcagca ggaatgtcga gtctcatatc tcggattgcc cgcattatga ttatcgcttt
     7681 aggatcgctt gtgcagaata cagcatccgg cctaagaccc ttttttaaaa gattttgcat
     7741 gagatagtag aaactattat ttcccgcggt ttccataagg acgagcgcgg gatcaaaagg
     7801 tatttcagca gctttgagag catcgacata cccttggtaa cgatgcttat agatcgatag
     7861 ctcgcgtggc ccacaggcga tagctatttt gcggtgacca gtgcgtatga gatagctcac
     7921 tccatcatat gcagcctgat agttatctat tatcactgca tcgattttct cctcatagta
     7981 tcgagagata agaactacgg ggaagccctc ctcatgaagt gctctgatat agtcactttc
     8041 tggaagcatt gaggcaacta taaatccatc aatatagcgg gtcttgagct tggcaacgta
     8101 ttccttttcg attgctatat cgtcgtcggt gttgcataga atagtagtaa aacctttctt
     8161 tcttgccaca ttttctacgc cgcgagcaat aacggggaaa atttcattat ggatgcttgg
     8221 gacaagcagg gctatcgtgt tggttttgcc caatttaagc ccttgcgcaa ggacattagg
     8281 ggaatatttc aaggtcttga ttgcatcgag aactttttcc ttagtctttt cattgacatt
     8341 gcccttatct ctcaataccc ttgagactgt gcttgctgag acaccagccc gaaggcccac
     8401 atctttgaga ttgatcacag ggctcaccct aaatcccagt agacgctcgc tccgcggtgg
     8461 gcagaggaac aattcttctt gtagagcgag agccatccag aagaaggttt ttcaatcctc
     8521 ttccaatatc gacgcctttc tgcgaattcc tcgtcggtca cgagggccat gagttttcgc
     8581 gcttctatgt cgatatcgat gatatcgcca tttttgagga gcgctatatt tcctccttca
     8641 tatgcctctg gcgatacatg gccgatcgcg agtccccctg ttgctccaga aaatcgccca
     8701 tcggaaacga gtgcaatttt ctttcctaag ccttttccta gaatagcagc cataaaggtc
     8761 tccatgtgtg gcatgccagg cgctcctttg gggccctcgt atcgtatgat cacaaggtcg
     8821 ccttcatgga tttcatcttt tagtgctgcc tgccacgctt catcctgaga ttcataaatt
     8881 cttgctggcc ctcgaaaatg ccaggcttcc ctatccactg ctgaaaattt gactattgca
     8941 ctttcagtgg caatattgcc cgatagcact gcaagtccgc cttcgaagag aatgggatcg
     9001 tctacagtat ggattacttc catattgtag tttctggaca gagcaatttt ttcagcaagt
     9061 gtgccaaaca tgcccttggc agatacatcg acaagccctt tgcgagccaa ttccgccatt
     9121 acagctccaa gccctcctgc ttcagaaaat tctaccatcg tatatttttc gtgattggga
     9181 tagatagtag aaataacagg cacttttcgg cttattttgt caaatacttc tggcgttatt
     9241 gcaatatctg cctgccgagc aattgcagga atatggagaa ttgaatttgt ggatccgcct
     9301 gtagccatca tgtaggccac agcattctcg aaattgggct gttttaccac atcaagaggc
     9361 cgtatttcat catggacgag ttccatgata cgacgagccg caacggcggc catctgtcgc
     9421 cactcatcgc tctcggcacg tacgctcgcg ttcccatgcg gcgtaagacc aagtatttca
     9481 gcgatcgcgc acattgtgtg tgcagtaccc atgaatgggc agattcctgg agtagggcaa
     9541 gcatggcgca ctatgtcttt atattcatca tggggaatag tgccagcgac ataggcagca
     9601 taggcctgtt ttgtgtgggt caatgtaatc attttgccat gatggttgcc aggaagcata
     9661 tatcctccgg tgagcatcac cgaagggatg tttgtcctga aaatacccat gagcattcca
     9721 gggaccacct tatcgcagct cccgaggagc accattccat caagcttgtt gagttctgca
     9781 atggtttcca cttccgaaga gacaagatcg cgcgcaggca gagtataacg gtcgccagga
     9841 gtattggagc atattccatc gcagataccg gtaacgggga gttcgcgagg gagccctccc
     9901 tgagcgttaa tttcgccttt tatggcatca aggatatgct tgaaatgaaa atgccctgga
     9961 ttcatctcat tccaagaatt tataatccct atgacggggt tctttgcttc ttcttcgcga
    10021 atgcccatag cataaaaaag cgcatttcta tggtcgagcg attcttcgtt ccattcttgt
    10081 atcatgatag aggactccct aatcttctct tttcaggaga tgaatcgcga aaccagcgat
    10141 ttcatcctta aaatagatta ctgtcatatc actattttga ttgcgcttta tggaatcttc
    10201 tgcaaaaaga aatccttgcg attcgaacca tctctttgct ccttcgatat catttgtcgc
    10261 tatagcgata tgaccatgtg ttcctctccc cggagctttc ataagttcga taaagtcgcc
    10321 tgcataaagc gaacttctgc tttctttccc taccttctga tggaataaag aagaaaaaag
    10381 ggctgcagtc ttttttgctt cttgtgcatt aggaagattt attccaatat gcgctatctc
    10441 gaagctgaaa tggtcttcag aaatttgatt tgatgattcc atattctcaa atacctcatt
    10501 cgccaagata ggctttcttt actgcgggag aagcgagaag ttcagcacca ctcccacaaa
    10561 tagcgattcg cccattctcc agtacatagg ctttatcaca cgcacagaga gccttctgtg
    10621 cattttgttc gacgaggaga atagtgatgc ctgaattcct tatttgctcg acaaggtcaa
    10681 agacctgtct catcacgagc ggtgcaagtc ctaaagatgg ttcatcaagc agcataagct
    10741 ttggatctga catgagacca cgacatatcg caagcatttg ctgctctcca cctgaaaggg
    10801 tacccgcgta ttgattgcgc ctctgttcca aaatggggaa cagatcgtat gctctcctga
    10861 gattctgcag tagtttcttt cctctaataa gatacccgcc tacaagaaga ttatcctgga
    10921 ttgtcagtcc agagaagcat tttcttccct cggggacatg gacaataccg gcagccacat
    10981 tgagatgagg cttttgggaa ataggtttcc ccataaattc gatgcttcct gaggtgggtt
    11041 ttatcaatcc tgatatacaa cggagtaagg tagtctttcc cgctccgttt gagccaataa
    11101 tacttacgat ttggcctttt ccaatatcaa tggaaacatc tctaagtgct tcgatatgcc
    11161 cataggatgc gcttagattt cttatgtgca acatcaattg cattcctctt caccaagata
    11221 agctttgata acagccggat ttgactttat ttcggaaggg cttcctatcg ctatagtgtg
    11281 gccaaaatct aaaacatata tttcttcaga aaggcccatc acgagttcaa gacgatgctc
    11341 aataacaaga atgcccaatc ctcgttcatc ccgaattcta tggatgagtt ccatgagatc
    11401 gcttacttct ttgggattga gacccgctgc tggttcatcg agcaagagta tttttggctt
    11461 acaggcaagg gcgcgcgcaa tttcaagtcg tctttgtaga ccataaggaa gattctctgg
    11521 tttctcgtcc ctgtatttca atagatcaac aagttggaga taatcttcac tcaatttatg
    11581 tcctcgtttt tcttccctta tcctcatagg cgtagggaaa agaccaccga aaataccata
    11641 gcgagaagta ggatcgaaag cgcacattac attatcaatg accgataacc ctgtaaaaag
    11701 tcttatattc tgaaatgtcc tcgcaatacc tgcacgcgtg ataatgaatt gctcttttcc
    11761 tgaaatatcc ttatcattca aatatatttt gcctttatct gggggatata ctccagaaat
    11821 aacgttgaag agcgtggtct ttcctgcacc attaggacca atgataccaa ttatctttgc
    11881 gcgatccatt gaaagagaaa aatccacaag tgcgtgtaca cccccaaagc tcttactgat
    11941 tttttctatc tgtagtagca tttataactc catttttatt gccttttttg aacaatttgg
    12001 caataaatgc agggctgagt tcgaactctt cgaataagcc agagggttta aaattgatga
    12061 tgagaagcac aagaacagaa taaaaaaaca tgcgatattc gcttgcaaag cgcagcattt
    12121 caggcagagc tgataataga agagtcgcga taatggaacc agtgaggcta ttcactcctc
    12181 caaagaatac catgattacc cactctgcgc tcttcctcca tccaaacatg ctgggatcga
    12241 catatgtcgt ataaaatgca taaagaacgc cagaatatgc agtgagccct acggagagca
    12301 gaaaggcaat cattttcatt ttaggtacat ctatacccat ggcctttgct gcaagctcat
    12361 cgctctttaa ggcaatgcat tgacgtccat atttagagat cttcaagaaa gcaacaagcg
    12421 taatagatat aattaccgag ataatgacaa gagcaaaatt ggttttttga gggatgccag
    12481 aaaagcccat cgctccaccg gtgatctgaa cagactgatt gagcagtgcg gtgatggctt
    12541 ctccaaaacc gagagtcacg agagaaatgt aatcgcgacg gagccgcacg gtaggtaggc
    12601 caacaataaa gccgatcatt atgcttacca acaccgcgat gatggtagca ggccaaaatg
    12661 gcatattaag cctcaccacc agcacccctg aaatatatgc accaatcgcc atgaaagaag
    12721 cttggcccaa agagaacatt ccggtgagac ccgtgagcac atatatccca cagactgaga
    12781 ctaccgagat aagcgcaaat gtgaatactg ataaatataa ttccattgat gtactcctca
    12841 ggctttttct tgcacgttgg acccggatat tccttgtgga tgtacaagca gaaatacaag
    12901 cattatgaga aaagtaaaca ccggcgcata tcctgatcct ataaaattga taagcagagt
    12961 ctcagtgagc cctaagagca ccgcacctat cacagcacca gacatcgacc ccaatcctcc
    13021 aatgaccgaa gcgataaatc ctttggtcac taaattgcct agctgcggat agagcgtata
    13081 attgattcca agaaaaactc cagaaagccc gcctaaggct cctgatagga aaaaggccag
    13141 ctgaatgtgg tgtatagtgt ctattcccat gagactcgcg gtgccacggt caaaagaaac
    13201 tgctcgaaca gcccgcccta tccttgtttt tgcgagcatc accactaaca gaccaagagc
    13261 aagccatgaa gtcagaaaca tgatcatgtc tgatcgtgaa atcactatat tgccaataga
    13321 tatcactagt tttttaaaga atcgcggata gttataaaag ttcggccctg ctccaatagt
    13381 cacaatggct tcatataagg atcctaaagt gattgaggat acaaaataat agatcggaga
    13441 agcttttctg acaacaagcc ttcgaaatgc gatgagttcg cccaggatag ccaaaatgcc
    13501 tccagaaagc atggaaacga ttatgacagg gaaaaggcct aatttcgtct ttattgcgaa
    13561 caaatagcct agaaaagcac ttgcggtcat aatactgcca tgagcaaaat tggagaattt
    13621 cagtatgttg aagatcaacg cgaatcccac tgcgattaag gcatacactg aacctaaagc
    13681 gataccattt attatgattt ggagcgacat ctaaattcct ttagcgcact aagtaaagct
    13741 cgcgcttccc gctcaaagga gcgggaagtt ttattataag gctattcctc catgatgatt
    13801 tatttcttag tgggggctgt ataccgtttc agtactacag gctgaccatt tttgatttcg
    13861 tgcataatca tctcaagccc tattgtctga tgagtggccg gattaatcgt aatcgtacca
    13921 gtaaggccaa catagccagt gaggttttct acagcctttt gtactgcttc agggtcgtcg
    13981 cccactgttt ccactatctt tgcgatgatt ttcataacat cgtagccaag gaagaatttg
    14041 ttcgtcgcaa tgttgcctgt tttttgcaga tatgcctggg agatatcctg aatttgtgga
    14101 tcactctcgg tgatattgtt gatataaatg accccgtcgg cctcgggacc gcaaagagaa
    14161 gcgaacggtg ggcaggcatc tagtccaaag ataagaggac cattgaaccc aattgcgcgg
    14221 gcttgttgtg agatgaggat gagttcctgg gtgtagttag gggcgtaaat tgcatcgaca
    14281 ttggctgcct tgatcttgct gagcattgtt ttgaaatcct tgtcgttcgg ctgataagga
    14341 acttcaatgg ccacattggt acctggtaga tttttgagag tatccttaaa gggccccacc
    14401 agcgagactg agtacgcatt cccttggtta tagataatgc cgaaattctt aagattgcgc
    14461 tctctcacag caaagtcagc cataattgcg ccttgctgat ccgaagaagg ctggaaaagg
    14521 aacatgttct tgtaaggttg tccggatggc ttgatggtgg ctctatcatc aattgcaaag
    14581 cccagcacag gcactttgta ctgctcagaa ataggggcaa tagcaattcc aatattggca
    14641 actggtggtc cgatgatcgc gctgaccttg tccgaggtac ataagcgctt gaatgcattg
    14701 atggcttctt gcacgtcgcc tcgtgtatcg tacacaatca atttgacctg gcgcccttta
    14761 ataccgcccg atttgttgat ctcatcgaca gcccattggg ccccctcctt aatcatcttg
    14821 cccagcgttg aagtcggacc agtgatatcc tgcagacaac cgacgagcac ttcgtttgat
    14881 gcaggcgctg catttgcgct gactgcgact gcaagcgcaa gcaagagaat tgcaatcgtt
    14941 ttcttcatcc ttgcacctcc tagaaaatta tgaaaatcaa tatagagcac tcttcatctt
    15001 ataaaataaa gaattaagtg ctcattttga tttttattaa agcaaagtat aagcccatta
    15061 ttcttcgctg tcaataaaat cttcacaatt tatgcaaagg ttttcataac gagagctcca
    15121 tgacggtgat tgaaaatgcc gtggagagag aagctgaggc aatgatgccg ttctcactcc
    15181 tgagttctca tttttttaaa aaatcgcaag attaatcaaa aattttgaat tatttttcaa
    15241 aacactcttg ccaaagaatt tttggtcgat tattatacgc caattcaagg ttggttggta
    15301 ccgccttcgg aggcaggcaa tgaaacggca cgtggtgtcg gcacagggaa aacccacatg
    15361 gcaatcgccc tcggcattgc cgcctgcgaa aaggggcttt cg
//
LOCUS       DAOOWY010000042        15270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3906_length_15270_cov_9.809004, whole genome shotgun sequence.
ACCESSION   DAOOWY010000042 DAOOWY010000000
VERSION     DAOOWY010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15270
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3906_length_15270_cov_9.809004"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PK105_04765"
     CDS             complement(<1..330)
                     /locus_tag="PK105_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61053.1"
                     /translation="MKKNNRESWFFELKKSLRSVQPPLWFFFVAIAIAAIGLILVGPS
                     SISSRSSLKAIKVGEIAETDIYAGKDTIYIDKEATQRKIQAEERLVLAVFVMDDKITA
                     SIREKAEE"
     gene            complement(317..1309)
                     /locus_tag="PK105_04770"
     CDS             complement(317..1309)
                     /locus_tag="PK105_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOU61054.1"
                     /translation="MSPSHIVLEDTEVFAELCGANDYNLSVIGSLLGTHVLTRGNELF
                     VDSDDPGILNLFSRIISAIGDSIKEGVPATPELIIALHAELTPDGSQSFKATIDNADE
                     DNESVISRFLENCIQIPNGFGKVFPRSSGQSLYLKGIASHDLIFSIGPAGTGKTYLAM
                     AYALRELLTRSKRKLILTRPVVEAGESLGYLPGDLEQKINPYLRPLYDAMEALVPYET
                     IRRLEESRAVEVAPLAYMRGRSLANCIVILDEGQNTTKEQMKMFLTRLGEHTKAIVTG
                     DITQIDLPKKTDSGLIHAITLLGNIDEVFISRLDASDVVRSSLARKIIEAYEKE"
     gene            complement(1485..4460)
                     /locus_tag="PK105_04775"
     CDS             complement(1485..4460)
                     /locus_tag="PK105_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61055.1"
                     /translation="MRRIIGIIFIFRILLVPCAAQAQIQNDNRAYKPEEVVQKSDSDI
                     TLTIEQAASAEYFSLENGTKELRLTGPLIIRLIDSGGVTHSIKAQYVVYNRDTKEVKA
                     YGDVEYTRRTESKTDIYKGESIAVDLDDNSGVFIDGSFDMESTTPGSRKLIVHFESLI
                     SRSEEVIALANGSLTACDAIKPHYILRAKKIWLFESGDWAASNAILYVGAIPVIWLPM
                     FYYPSKASKFHPVVGFRSRAGAFLQTTTYLKGAQSSEVQKSSLLSTTEGTSGSFGSYI
                     SKTKPADESKNQTNIAILGDVYSALGVFAGLRGELANTYPYDISWLIGAAFSRSVFLE
                     STGYYSPYDSAGDYASVWNRWKLSSIDLPMRILMNFEASSKKNKAGIKWQISLPFYSD
                     PFVEQDFMDRNESYDFFSILGSSSLKTIDERSSFSQKASLSWSWSPFIEKNSFAFTLS
                     NLSSSFNWKRKYASTAGMNPTEKRLNAVDPQRYFFYPDSARIIDTAFSMSGTILQSKN
                     MSLKWTNTNSSFLEDRFYNAKWQKPQDIDFESWYWLYGGRSNTNLSSTYSIDSVGLTF
                     QISTGILGQLQYRPYLYDERVNATTVHPFLIADYSYNTANWNTGTKLSWEPFIKSDTF
                     SASKVSYSIQGKVAKLAYEGLDGSGVDAYPIYKLYWLSWDPNMISDHSILAELAAKLG
                     STTERLSFKATLPPILESYTLSGSSALGFATVGASYVISRKSSLEDLKSTSLMGNLAL
                     RPIKKIEISANAAWDFDTQAPLSLSANLSAWSFNARFVAQKANGYVFKNSSWIQDGTQ
                     YFRPSTLSLSWKPKIRYSSPLFSTEMMSWYVEADGLLTLNQNLIQFTNSTFGANLGLS
                     IKNSAGLSLGFTLSSVNSSFWRYYASLFPKSDDFDPEDYSKNLFADLWDSVSIWDANK
                     LKNTLFKLQGLKLELSLDAHDWVLSANILAEPTLVTASSTQPLPHYEMEVAFNLAVTW
                     KDISAIKSSINYSEGAFKQ"
     gene            complement(4520..7342)
                     /gene="uvrA"
                     /locus_tag="PK105_04780"
     CDS             complement(4520..7342)
                     /gene="uvrA"
                     /locus_tag="PK105_04780"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU61056.1"
                     /translation="MDKLIIKGAREHNLKNIDLELPRNRLIIISGLSGSGKSSLAFDT
                     IFAEGQRRYVESLSAYARQFLGRMDKPDVDYIEGLSPAISIEQKTTSRNPRSTVGTVT
                     EVYDYLRLLFARIGVPHCPSCGREIREQSVDQILDVILSWPMGTKIQVLAPVIRSQKG
                     EHKKILEDALRQGFLRARIDGQFIDIESPPVLEKQRKHTIELVVDRILLSEESRRRVA
                     EAVETSLGIANGIVIIVREMDGQRKEDFFSQKGACPICGISLPEMEPRLFSFNAPQGA
                     CPACSGLGMNMEFDPSLVIPDRSLSFNEGGCLAYNPNSAWNRSRFEALADHYHFSLDT
                     PFDKLPSYVMNAILYGSDDNIHVRYNNRDKTGYFEYESRFPGILADLRRRYMESTSND
                     IKDWLESFMVEKECEVCHGKRLKPEALAVTIGSKNIYEISCMPVEDSVQFFKSLRLTD
                     TEAQISKLILKEIIARLGFLQNVGLEYLTLERQASTLSGGEAQRIRLATQIGSSLVGV
                     LYILDEPSIGLHQRDNQRLIDTLTYLRDLGNTLIVVEHDEQMLRTADYIVDLGPGAGE
                     HGGYIVAQGSVQEVVANPQSITGQYLAGSLKIEVPIERRKGNGACITLKGASEHNLKN
                     IDVKIPLGRFVCITGVSGSGKSTILSDVLYPAISNRVMHSAIREGSYKSLEGIEYIDK
                     IINIDQSPIGRTPRSNPATYVGVFTPIRELFASLPESKARGWKPGRFSFNVKGGRCEH
                     CQGDGTIKIEMNFLPDVYITCEVCHGKRFNSDTLDVRFKGKNIADVLNMTVEEARTFF
                     AHIPSIAHKLQTMVDVGLGYIRLGQSALTLSGGEAQRVKLSLELARRATGRTLYFLDE
                     PTTGLHFADVRQLLEVIRRLVDSGNTVVMIEHNLDVIKQADWIIDLGPEGGERGGMVI
                     AEGTPEEIALCRDSYTGQYLRRLLNI"
     gene            complement(7417..8523)
                     /gene="ychF"
                     /locus_tag="PK105_04785"
     CDS             complement(7417..8523)
                     /gene="ychF"
                     /locus_tag="PK105_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOU61057.1"
                     /translation="MPLNCGIVGLPNVGKSTIFSALTRAKVDTANYPFCTIDPNVGIV
                     PLPDKRLDRLFDIFKPKRKVPAVVEFVDIAGLVRGASKGEGLGNQFLSHIREVGMIAH
                     VVRCFDDPNVVHISGKIDPADDIEIIHTELALADLESIDRRLDKLSRQVKVQDPNLKK
                     DIEKEIAVLQKLRPILEEGRPASTAILEPEEVDYARRSFLLTLKKELFVCNVDESGMA
                     NNKYVDIVKSIAEKQGSEAVVICGKYEAELADIEDSEERAEFLHSIGLEEPGLDVLAH
                     AAYRLLGLRTFFTTGEDECRAWTIKAGDKASTAAGVIHSDFEKGFIKAEVYHYNDLID
                     YGSETAVRAAGKMRQEGRDYVVQDGDIMFFKFNV"
     gene            8708..9955
                     /locus_tag="PK105_04790"
     CDS             8708..9955
                     /locus_tag="PK105_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOU61058.1"
                     /translation="MHDLSVYLSANAHNMKKSVIRELLKLTNQPDIISFAGGLPAPET
                     FPVEDLREASDQVFRKYGDKILQYGTTEGDNDLKARIIEYESSHGVNLDPDNILITSA
                     SQQALDMVPKLFLDPGEYVIAERPTYLGAIQAIQSYQGKVLGIPFSHANDGLDMDELE
                     KEYARAKAENKRIKYIYVIPDFQNPTGVCWSLEKRKALIEFAYREELFIVEDAPYREL
                     RFMGESIPSLYQLELEMQNLGIVVGLKTFSKILAPGTRIGWILARKDIIQKLVVAKQA
                     VDLCTSPLTQKIVAQFLATGKLEEVLKNTCAIYRDKRNFMLQKLETYMPKRPDLTWTK
                     PEGGLFLWLSLPSYVDTDKMFYKAIEKKVAYVVGSSFYYDDPEVNSMRINFSYSSYEQ
                     IEEGVKRLADTFKEEIEEHEGHL"
     gene            10209..10946
                     /locus_tag="PK105_04795"
     CDS             10209..10946
                     /locus_tag="PK105_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61059.1"
                     /translation="MPIIQVIDVKKDYYLGNVVVPALRGISFAIERGDFVSIAGPSGS
                     GKSTILNIIGLIDIPTSGELIINEKNTKGLSDRELTRFRHEVLGFIFQSFNLIPVLNV
                     YENIEFPLLLGKTKIPKSEWKSWIDFLIDEVGLSDWRNHKPNELSGGQRQRVAIARAL
                     VTKPSIVLADEPTANLDSTTGEQIIDLMKKINRELETTFIFSTHDAKIVNIADHVIRL
                     RDGMIIGNKRNGEDILEENASIASAEN"
     gene            10949..12451
                     /locus_tag="PK105_04800"
     CDS             10949..12451
                     /locus_tag="PK105_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU61060.1"
                     /translation="MPIIFKIAFRNIKAHRSKSLIIGVLLAMGAMVLVLGTAFINATQ
                     EGVRSTFSDVYTGDIFISGISSDGPVSLFGVTSTSGMATTPIIPEYDKVISIVKSTPH
                     LEKTSSLATGFSQVIRENGDQETEEDSQKKIKPQDRFLFLFGIDAASYWDLFETVEIV
                     AGEKLKPGQSGIMINESQFHKFSDWFEKPLSIGDPLLIQGFSTIGFRLRELPIVGIFR
                     LKEQGTAPEQLAYIDIESLRVMSGMTVGANEPIQLKPEQTSMLSIEDPDELFSDDIIV
                     TSETSKHFDVASISRELSRPSEIDPLKPDEGAWQFIVGKADSSQNIDRIISSLNKAFE
                     KEEIQAIAGNWQVAAGPYGQSVDIVRIIFAVAIVILSIVAIIIIMNTFVISVIERTSE
                     IGTMRAIGAGKGFVRTMFAAEALALALVSASIGAGLGLLIIEILKSLRIEATNPFFLV
                     LFGGQYLRPIATAGNVIFAIILMIVVGFIAHLYPVSLALKIQPVRAMQEE"
     gene            12462..13787
                     /locus_tag="PK105_04805"
     CDS             12462..13787
                     /locus_tag="PK105_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU61061.1"
                     /translation="MNLVLLRIAARNLMRQRKRTMLLAGAIAFGIMIVTILNGFAGSF
                     VKNISDNFSYLMGGHVFVSGTEVTPSGKRISIIRDDSVIMQALEDSGQKWVSLQKTSQ
                     TIATLIFEGKSIQQTLTGIDLTHSGMIKERLALVDGNWENLSWSNAIIISQSIARKLN
                     VLPGDVLVAQLQTITGQNNVGEFRVAAITIDSSFVGSIMTYVQLEYLNSLIGLGKNEY
                     MSLGIMLEKLEKSGAFANALYTSMKGMGLNLFDRSTKDENGSMTPFQAMLLEQRTEQW
                     TGTKYRLYTIDDVLAQAKQIVVAIDTGSTVILIVLFAIVMIGISNTFRMAMYERIREI
                     GTMRAIGVQRNEIRSMFLYEALLLSLAGAIAGIVLALIAMGLLTLISFDPLSPVFLIL
                     KRGHFSFYVPPLRAIGNILLIAVLTLVAVYAPANAAAKLPPAEALRTVK"
     gene            13804..14601
                     /locus_tag="PK105_04810"
     CDS             13804..14601
                     /locus_tag="PK105_04810"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOU61062.1"
                     /translation="MYSNVSRKAKSLRRRILSILSLLLLFFTGLNPLLAQSKPDPREL
                     IKQVDDLSDFSGKDFSAVFTIVTQKPGEKDSVTQARVFRRDLKKQFVILILLPEVNKG
                     QGYLREDDNVWFYDPTSRKFSHSSMKENIQNTKAKNSDFTLSTFAEDYTVTKMTESMV
                     GKFPVWVLDLKARTNEVAYDRVVLYIRKDRTMLLKREDYSVNGRLMRTTAYPKYVELE
                     GKLLPSQILIVDEVNKGEKSQMTMTEQSVSPLPDRVFTKAFLEQVSR"
     gene            14631..>15270
                     /locus_tag="PK105_04815"
     CDS             14631..>15270
                     /locus_tag="PK105_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61063.1"
                     /translation="MSLLRNLGAIGILCVIIMAAPFSISSQEESNDIDALFEDEIIVQ
                     QDSDTLGQEESQDSFIGQTNPLQSFLKTETVKIGGNISGTVSASWKWNDPWTVGFDLA
                     NPDSHKLAPALNSLLYFDARPEEDFRVHGSFKTAWPFAETNSLLDSNNKTTLVSVPDV
                     RIFELFSDFQLGDNAYLRFGKATVKWGVGYFFSPADIINLESINILDPTAQRE"
BASE COUNT         4290 a         3462 c         3316 g         4202 t
ORIGIN      
        1 ttcttcagcc ttttcgcgaa tagatgcggt gatcttgtca tccatcacaa agacagcaag
       61 caccaatctc tcctcagcct gtatctttct ctgtgtcgcc tctttgtcga tataaatagt
      121 gtctttgcca gcataaatat cggtctcggc aatctcacct acttttattg ctttaagcga
      181 gctacgactt gaaatcgatg atggtccgac caggatcaga ccaattgccg ctatggcaat
      241 tgctacaaag aagaaccata agggcggttg taccgagcga agagattttt tcagctcaaa
      301 gaaccaggat tctcgattat tctttttcat aggcttctat aattttcctg gctagagagc
      361 ttcgaaccac gtcgctagca tcgagcctgc ttatgaatac ttcatcgata tttccgagca
      421 gagttatggc atgaataaga cctgaatctg tcttcttcgg gagatcgatc tgtgtgatat
      481 ctcctgtaac gatcgctttt gtgtgctctc caagccgagt aaggaacatc ttcatttgct
      541 cttttgttgt gttctgacct tcgtccagaa tgactatgca attagcgagg cttctgccgc
      601 gcatataagc aagtggtgca acttctactg ccctgctctc ttcgagtcgc ctaatggtct
      661 cgtatgggac caatgcttcc atagcatcgt agagggggcg gagataggga tttatcttct
      721 gctcgaggtc acccggaaga taaccaaggc tttctcccgc ctctactact ggtcgcgtta
      781 aaatcagttt tctcttcgat cgcgtcaaga gctcccgcag cgcataggcc atggcgagat
      841 atgttttgcc ggtccctgct ggccctatag aaaagattag gtcgtgtgac gcaatgcctt
      901 taagataaag actctgtccc gaagaccgtg gaaatacctt accaaaacca ttcggtatct
      961 gtatgcagtt ttcgagaaac ctggaaatta cgctctcatt atcctcatca gcattatcta
     1021 tcgtagcttt gaaggattga cttccatccg gtgtaagctc cgcatgcaga gcaataatga
     1081 gctccggagt agccggtaca ccttccttta tcgaatctcc aattgcgctg ataatgcgtg
     1141 agaaaagatt gagtattccc gggtcgtcgc tatccacaaa aagctcattg ccccttgtta
     1201 gcacatgagt ccccaatagg ctcccaataa cagatagatt gtaatcgttt gcgccacaaa
     1261 gctcggcaaa tacctcggta tcttcgagca caatatgtga aggactcaat cgcactcctg
     1321 tctaagtata atattcttta ataatatact aatacctgga ggctctttcc agccttcaga
     1381 tctcatccgc acttttggct gttgtttctt ttctcttgac tgttggtctc ttagatcttg
     1441 agcctctatg ctcgacgaat tcccaataag actagggctt atattcattg cttgaatgca
     1501 ccttctgagt aatttatcga actctttatt gcagagatat ccttccacgt aaccgcaagg
     1561 ttgaatgcca cttccatctc gtaatgtggc aatggttgag tcgaagatgc cgttacgagc
     1621 gttggttcag ccagtatatt tgcggataat acccaatcat gagcatccaa tgaaagctct
     1681 agctttagac cctgcaactt aaaaagagta tttttaagct tgttagcatc ccagatagac
     1741 accgaatccc aaaggtcagc gaagaggttt ttagaatagt cctcgggatc aaaatcatct
     1801 gattttggaa acagagacgc ataatatcgc cagaatgaag agttcacaga agacagcgta
     1861 aaacctaaag ataaacctgc agaattcttg atggaaagac caaggtttgc tccaaatgta
     1921 gaattcgtaa actgaattag attctgattg agagtaagta atccatctgc ctcgacatac
     1981 cagctcatca tctcagtaga aaatagcggt gaactgtatc gaattttggg tttccaagat
     2041 agagataatg ttgaaggcct gaagtactga gtcccatcct gaatccatga actgttcttg
     2101 aaaacatagc cgtttgcttt ttgtgccaca aagcgtgcgt tgaatgacca agccgacaga
     2161 ttggcactga ggcttaaggg agcttgagta tcgaaatccc aggcagcatt cgcagatatc
     2221 tcgatttttt ttattggcct aagagcaagg ttacccatca atgatgtcga ttttaggtct
     2281 tccagtgagc ttttgcgaga aatgacatag ctggctccca ctgtcgcaaa accaagagcc
     2341 gatgaacccg aaagagtgta gctctcgaga ataggcggta gagtggcctt gaaactcaag
     2401 cgttcggtcg tggacccaag ctttgcggcc agctccgcaa gtatagaatg atcagatatc
     2461 atatttggat cccatgataa ccaatacaat ttataaatag gataggcatc tactccgctg
     2521 ccatcgaggc cttcgtaggc caatttggct accttccctt ggatagaata agagactttt
     2581 gaggcagaaa aggtatcgga tttgatgaac ggttcccatg acaacttggt cccggtgttc
     2641 cagttagccg tattgtaact gtagtccgca attagaaaag gatgtacagt tgtggcattt
     2701 acgcgttcat cgtagagata gggtctatat tgcagctgcc ctaatatacc ggtcgaaatc
     2761 tgaaaggtca atccaacgct atctatcgaa taggtagagc tcagattagt gttgctgcgg
     2821 ccaccgtata accaatacca gctttcaaag tcgatatctt gaggtttctg ccatttggca
     2881 ttatagaatc gatcttccaa aaaactggaa ttcgtattag tccatttcag cgacatgttt
     2941 ttgctctgca gaatagtacc agacatagag aaagctgtat caataatgcg cgcactatcg
     3001 ggataaaaga agtatctttg cggatctacc gcattgaggc gcttctctgt aggattcata
     3061 ccggctgttg aagcatattt tcttttccaa ttgaaagatg acgatagatt ggagagcgta
     3121 aaagcaaaac tatttttctc aataaaagga ctccacgacc acgaaaggct tgctttttgt
     3181 gaaaaagaac tgcgttcatc tattgtcttg agggaagaac tgccaaggat agaaaaaaaa
     3241 tcataggatt catttcgatc cataaaatcc tgttcaacga atggatcgga ataaaaaggc
     3301 aaactgatct gccactttat tcccgcctta ttctttttag aagaggcttc gaagttcatt
     3361 agaatgcgca tcggaaggtc tatcgatgag agcttccatc tattccagac cgaagcatag
     3421 tcaccggcac tatcataagg agaatagtaa cccgtcgatt ccagaaaaac agaacgcgaa
     3481 aaggcagctc caataagcca agaaatatcg tatggatagg tatttgccaa ttctcctctt
     3541 agcccagcaa aaacccccag ggctgaatag acatctccca aaatcgctat atttgtctgg
     3601 ttcttgcttt catcagcagg cttagttttg gaaatataag aaccaaacga gcctgatgtt
     3661 ccttcggtag tggatagcag ggaagatttc tgtacttcag aactctgtgc tcccttaaga
     3721 taggtggttg tctgcaaaaa agctccagca cgagatctaa aaccgaccac tggatgaaat
     3781 ttcgatgcct tagagggata atagaacata ggaagccata tgactggtat cgcacccaca
     3841 tatagtatag cgttcgatgc tgcccaatct ccagattcga acagccagat cttttttgct
     3901 cgcagaatat aatgaggctt tatagcgtcg caggcagtaa gagagccatt ggctaatgca
     3961 atcacctctt cggatctgga aatcaaagac tcaaaatgta ctatgagttt gcgggaacct
     4021 ggggtggtag actccatgtc gaaagaacca tcgataaaga caccagagtt atcatctaga
     4081 tctacggcta tggattctcc tttgtagata tctgttttgg attcggtccg acgcgtatat
     4141 tctacatcgc cataagcttt tacttctttt gtatcacgat tatagacgac atactgagcc
     4201 tttatcgaat gcgtcactcc gccggagtca attaaccgga ttattagagg ccctgtaaga
     4261 cgaagctctt ttgttccgtt ttcgaggcta aaatactcgg cacttgcagc ttgctcgatg
     4321 gttaaagtta tatcgctatc ggatttctgc acaacctctt caggtttata agctctattg
     4381 tcattttgaa tctgagcttg ggcagcacaa ggcacaagca agatccgaaa tataaaaata
     4441 atgccaatta tgcgccgcat cgattcgtat ggtatatgga aaaatgcggg gttggcaaga
     4501 ataccatttc tctactagct catatgttca gcaatctgcg caagtactga cctgtatatg
     4561 aatccctgca aagggcaatt tcctcaggag tcccttccgc aattaccata ccgcctcttt
     4621 cacctccttc gggtccgagg tcgataatcc aatcggcctg ttttatgaca tcgaggttat
     4681 gttcaatcat gactacggta tttccactgt ctacaagtct acgaattacc tcgagcaatt
     4741 ggcgcacatc agcaaaatgc agccctgtag taggttcatc cagaaaatag agcgtacgcc
     4801 cagtggctcg gcgtgccagt tcgagagaga gctttactct ttgtgcttct cccccggaaa
     4861 gcgtcagggc agattgtccc aaccgaatat aaccaagccc cacatcgacc atcgtctgaa
     4921 gtttatgcgc aatcgaaggg atatgagcaa agaatgttct tgcttcttca accgtcatgt
     4981 tgagcacatc ggctatgttc ttgcccttaa aacgcacatc cagggtatcg gaattaaatc
     5041 gttttccatg gcagacttca caggtgatat atacatctgg cagaaaattc atctcgatct
     5101 tgattgtgcc atctccctga caatgctcac agcgcccacc tttgacattg aaactaaacc
     5161 tccctggctt ccagcccctt gctttcgatt cgggcaagct tgcaaacagt tccctgatgg
     5221 gtgtaaatac accaacatag gtggcgggat tcgatctagg agtccttcct atggggctct
     5281 gatcgatgtt gatgatttta tctatgtatt ctattccttc gaggctctta taggatcctt
     5341 cacggatcgc ggagtgcatc acacggttgg aaatagccgg atataataca tcagacaaga
     5401 tagtgctctt tcccgagcct gatacacccg tgatacacac aaaccttcca agaggaatct
     5461 ttacatcgat attcttgaga ttgtgctcgc tggcgccctt gagggtgatg caagcgccat
     5521 tcccttttcg ccgttctata ggaacttcga tcttgagaga acccgcgaga tattgccctg
     5581 taatgctctg gggattggca acaacctctt gcacgctgcc ttgagctact atatagcctc
     5641 cgtgctcgcc cgctccgggt ccaagatcca caatatagtc cgctgttcga agcatttgtt
     5701 catcatgttc tacaactatg agggtatttc caaggtctct aagatatgtg agtgtatcga
     5761 tgaggcgctg attgtctctc tgatgaagac caatagatgg ctcatccaga atatagagca
     5821 cgcccacgag agaactgcct atctgtgtgg caagcctgat tcgctgggct tcaccacctg
     5881 agagagtcga tgcctggcgc tcaagcgtga ggtattcaag ccccacattt tgcaaaaaac
     5941 caaggcgtgc aataatctcc ttgagaatca gcttagaaat ttgagcttca gtatctgtta
     6001 gtctgagaga cttgaagaat tggacagaat cctctacggg catgcatgaa atctcgtaga
     6061 tattcttgct cccaatggtc acggctagag cttccggctt caatcttttc ccatgacata
     6121 cctcacactc tttttcgacc atgaatgatt ctagccaatc ctttatgtcg ttgcttgtcg
     6181 attccatata gcgacgacgg aggtccgcca aaattccggg aaacctagac tcatactcga
     6241 aatatccggt cttatcccta ttgttgtagc gaacatgtat attatcatca gaaccataga
     6301 gaatagcatt catcacataa gaaggtagct tatcgaaagg agtatccagg ctaaaatgat
     6361 aatgatcggc tagcgcttcg aatcgtgagc ggttccatgc cgaattagga ttataggcta
     6421 gacaacctcc ttcgttgaat gacaagcttc tatctggaat aaccaagctg ggatcaaact
     6481 ccatgttcat gcccaatcca ctgcaagccg gacaagcacc ttgtggagca ttgaaagaga
     6541 aaagccttgg ctccatctct ggcaaagaaa taccacatat cggacaagcg cctttttggc
     6601 taaaaaaatc ttcttttctt tgaccatcca tctcgcgcac gatgatcaca atgccatttg
     6661 ctatgccgag cgaagtctcg accgcctcag caactctgcg gcggctctct tctgataaca
     6721 aaatgcgatc caccacaagc tcgatcgtat gctttctctg cttttcgagg actggaggag
     6781 attcgatatc tatgaattgc ccatcgatcc gtgccctcaa gaagccctgt ctaagagcat
     6841 cctccagtat ttttttatgc tcgcctttct ggcttcgtat taccggagct aaaacttgta
     6901 tctttgtgcc cataggccat gagagaataa catcaagaat ttggtcgaca ctttgctcgc
     6961 gaatctccct accgcaggaa ggacaatgag gaacacctat tcttgcaaat agaagtctca
     7021 gataatcata tacctcagtc accgtcccga cagtcgatct gggattgcga gatgttgtct
     7081 tttgctcgat cgaaatggct ggagaaagcc cctcgatata atcgacatca ggtttgtcca
     7141 tccgcccaag aaattgcctt gcgtatgcag acagagattc cacatagcgc cgctggcctt
     7201 ccgcaaaaat cgtatcgaaa gccagagagc tctttccaga acccgacaag cccgaaatga
     7261 taattagcct atttctcggg agttcgaggt cgatattttt cagattgtgt tcccgtgcac
     7321 ctttgatgat cagtttgtcc attgtttgaa aatcatactg tggtccctgg cataaagaca
     7381 agtatgcttg acagatgtat agctattcag gctctgctag acattgaact tgaagaacat
     7441 tatgtctccg tcttgaacca cgtagtcgcg cccctcctgt cgcatcttgc ctgctgcccg
     7501 tacagcagtc tccgaaccat aatctataag atcattgtaa tgatagactt cagccttaat
     7561 aaagcctttt tcgaaatcgc tatgaataac gcctgccgcc gtcgatgcct tatctccagc
     7621 cttaattgtc caagcgcgac attcgtcttc tcctgttgtg aaaaaagtgc gaagacccaa
     7681 tagtctgtat gccgcatggg caagcacatc aagccctggc tcctcgagcc ctatgctatg
     7741 aagaaactca gctctttctt ctgaatcctc tatgtctgcg agctctgctt cgtatttgcc
     7801 gcagattaca accgcctccg aaccctgttt ttctgcaatc gattttacta tatctacata
     7861 tttgttattg gccattcccg attcatcgac attgcagacg aataattcct ttttgagagt
     7921 caatagaaag ctgcgccgag cgtaatctac ctcttcaggc tcgagaatag ccgtagaagc
     7981 cggtcttccc tcttccaaga taggccgcag cttttgcagc acggcaattt ccttttcgat
     8041 atccttcttg agattagggt cttgaacctt gacctggcga ctcaacttat ccagccttct
     8101 atcgatgctc tccaaatcag ccaatgcaag ttcggtatga ataatctcga tgtcatcggc
     8161 tggatctatc ttgcctgata tatgaaccac attaggatca tcaaaacatc gtaccacgtg
     8221 tgctatcatg ccaacttcgc gaatatggga aaggaactga ttaccaagac cctctccctt
     8281 cgaagctcct cgaacaaggc ctgcgatatc gacaaattcg accaccgcag gtacttttct
     8341 ttttggtttg aatatatcaa agagcctatc caagcgctta tccggcagtg gaacaatacc
     8401 gacattggga tctatcgtgc agaaaggata attcgctgtg tccacttttg cccttgtaag
     8461 agcggaaaaa atggtggatt tgcccacatt gggcaaaccc acaatcccac agttgagcgg
     8521 cattagggca ttcctctaat tgctgtattg attaccaaag tatagcaagg acatattata
     8581 caacaagtac catgcccgcc atctagaaaa atgaaactca ggaatataat tttgaaaaca
     8641 tatatttacg aatcataaaa actgaagtag gatgaaacaa gaaccacaaa tcaaggagga
     8701 cttatcaatg catgaccttt ctgtgtattt gtccgcaaat gcccataata tgaagaaatc
     8761 tgtaatcaga gaattgctca agctcactaa tcaaccagat atcatctcat ttgctggtgg
     8821 gcttcctgcg ccggagacct tccccgttga agacctcaga gaagcaagcg atcaagtctt
     8881 tagaaaatac ggtgacaaga tcctccaata tggcacaacg gaaggcgata atgatctcaa
     8941 agccaggatt attgaatacg agtcatctca cggcgttaat ctcgatccag ataatattct
     9001 cataacttcg gcgagccagc aggctctcga catggttcca aaactctttc tcgacccagg
     9061 agaatatgta attgcggagc gtcctaccta tcttggagcc attcaggcaa ttcaatccta
     9121 tcaaggaaag gtgcttggca ttcccttctc tcatgccaac gatggcctgg atatggatga
     9181 actcgagaaa gaatacgctc gcgctaaggc tgagaataag cgcataaaat acatctatgt
     9241 aattccggat ttccagaatc ccaccggagt atgctggagc cttgaaaaac gcaaggctct
     9301 tatagaattt gcatatcgag aagaattatt catcgttgaa gacgctccat atcgagagct
     9361 tagattcatg ggtgaatcca ttccttcctt gtaccaacta gaactggaga tgcagaacct
     9421 tggcattgtg gttggcctta agaccttctc taagatactt gcgcctggta caagaatagg
     9481 atggattcta gcgcgtaagg atatcattca aaaactggtc gttgcaaagc aagccgtgga
     9541 tctctgcaca agccccctca cgcagaagat tgtcgcccaa ttcctggcaa caggcaaact
     9601 cgaagaagtc ttgaagaata cttgcgccat ctatcgcgat aagcgcaatt tcatgctgca
     9661 aaagctggaa acctatatgc ccaagcgtcc agatctcact tggacaaaac cagaaggtgg
     9721 cttattcttg tggctctctc tgcctagcta cgtcgatacc gataagatgt tttacaaggc
     9781 aatcgaaaag aaagtcgcat atgtggtagg gagttcattc tattatgatg atcctgaagt
     9841 aaattccatg cgcatcaact tctcctattc gagctacgaa cagatagagg aaggagttaa
     9901 gcgccttgcc gatacattta aagaagaaat tgaagagcac gagggtcatc tctgaagcat
     9961 gagaccgatc tctggcgcat gaggttatct cttaggccat tatagtctaa gaagaaggat
    10021 tgagtcagaa tatacagctc tatattggat taagcgcaac gaacatgcaa agataggtct
    10081 atctgtatgc actcgaaatt ataaaatcgg tgataaaata atattttatt ttgaactcat
    10141 attgtcacaa cccatagaaa ttgctatttt tctctagcaa cagctatctt tttcaaaggt
    10201 gaaaaaccat gccgataatt caggtgatcg atgtaaaaaa ggactattat ctggggaatg
    10261 tcgttgttcc cgcacttaga ggaatctcct ttgctattga aagaggcgat tttgtctcta
    10321 ttgcagggcc ttccggctcg ggaaaatcga ccatactcaa tataattggc ctaatagaca
    10381 ttccaacttc aggtgagcta atcatcaatg agaagaatac taagggcctt tctgatagag
    10441 agttgactcg ctttcgacat gaagtactag gatttatttt ccaatccttc aatctaattc
    10501 ctgtcttgaa tgtctacgaa aatatcgaat tcccgctgct gctcggaaag accaaaatcc
    10561 ccaaaagcga atggaaaagc tggatagatt ttctcataga cgaagtaggt ctttccgatt
    10621 ggcgaaatca taagcccaat gagttatctg gaggacaacg ccagcgcgtt gcaatcgcaa
    10681 gagctcttgt tactaagcct tctattgtac tggccgatga gccaacggcc aacctcgatt
    10741 ccactaccgg cgaacagata atcgacctta tgaaaaaaat aaaccgagaa ttggaaacaa
    10801 cctttatttt ttccactcat gatgcaaaga tcgtcaacat tgccgaccat gttattcgct
    10861 tgcgcgatgg catgatcatt ggcaataagc gaaatggtga agatatcctt gaagaaaacg
    10921 catctatcgc gtctgcggag aattgagcat gcctatcatc tttaaaatag ctttccgcaa
    10981 tatcaaagcc catagatcca aaagcctcat catcggagtc ttgttagcaa tgggagccat
    11041 ggtattagta cttggcacag catttatcaa tgcaacccag gaaggagtgc gctcaacatt
    11101 ctcagatgta tatactggag atatttttat ctccggaatt tcgagtgatg gccctgtatc
    11161 tctgtttggc gtgacttcta caagtggcat ggcaacgacg cccataattc ctgagtatga
    11221 caaagtgatt tccatcgtaa aatcgactcc acatctagag aagacttctt cgctcgcaac
    11281 gggcttttct caggtaattc gcgaaaatgg tgatcaggaa acagaagaag actctcaaaa
    11341 gaagataaaa cctcaagatc ggtttctgtt cctttttgga atcgatgcag cgagctattg
    11401 ggatctcttt gaaaccgtag aaatcgttgc gggagagaaa ttgaaacctg ggcaatctgg
    11461 catcatgatc aatgagagcc aattccataa attctccgat tggttcgaaa aacctctttc
    11521 gataggcgat cctttgctta ttcagggttt ttctacaatc ggtttccgct tgagggaact
    11581 tccaatagtc ggcattttcc ggcttaaaga acagggtacg gcacccgaac aacttgctta
    11641 tatagatatc gaatctctcc gcgttatgag cggtatgact gttggagcca acgaaccaat
    11701 acaactcaag cccgaacaga caagtatgct ttcgatagaa gatcccgatg agcttttcag
    11761 cgatgatata atcgtgactt cggagacttc aaaacatttc gatgtcgctt caatctctag
    11821 ggaattatcc aggcccagcg aaatagatcc tctaaagccc gatgaaggag catggcagtt
    11881 cattgtaggt aaggctgatt cttctcagaa tatagatcgc atcatctctt ctttgaataa
    11941 ggctttcgaa aaagaagaga tccaagctat tgcaggtaac tggcaagtag ccgctggccc
    12001 atatggtcag tcggtagata ttgttcggat tatatttgct gttgccattg ttatcctctc
    12061 tatcgtcgcc atcataatca taatgaatac ctttgttatt tctgtaatcg agagaacaag
    12121 cgagattggc accatgcgcg ccattggagc tggaaagggt ttcgtgcgca ctatgtttgc
    12181 ggctgaggca ttggctctag cattggtttc tgcttcgatt ggggcaggac taggcctcct
    12241 gattatcgaa atccttaaat cgctccgaat cgaggcgaca aatccatttt ttcttgttct
    12301 atttggcggg caatacttaa gaccaattgc cacggcagga aatgtaattt ttgcaataat
    12361 actgatgata gtggtaggct ttattgccca cctttaccct gtttcgctgg ctttgaaaat
    12421 tcagccagta agagcgatgc aggaagaata ggagggaata catgaattta gtattgctcc
    12481 gcatcgctgc acgaaaccta atgcggcaaa gaaagagaac tatgctcctt gcgggagcaa
    12541 tagcctttgg aataatgatc gtaacgatcc taaacgggtt tgccggatca tttgtcaaga
    12601 atatctcaga taatttctcg tatttgatgg gcggccatgt cttcgttagt ggaactgagg
    12661 ttacaccatc gggcaaaaga atttcgataa ttcgcgatga ttcggtgatt atgcaagcat
    12721 tagaagattc tggacaaaaa tgggtcagct tgcagaaaac cagccagacc atagccacat
    12781 tgatcttcga gggtaagagt atacagcaga cgctgactgg tatcgatttg actcattccg
    12841 gaatgataaa agaacggctt gctctcgtcg acgggaattg ggaaaatctc tcctggtcta
    12901 acgcaatcat aatttcccag agcattgcta gaaaactcaa tgtcctgcct ggagatgtgt
    12961 tggttgcaca gttgcagacc ataacaggcc aaaacaatgt tggagaattt cgagtcgctg
    13021 caataaccat tgattcaagc tttgttggct ctattatgac ctatgtccaa cttgaatatc
    13081 tcaatagtct tataggtctg ggaaaaaatg aatatatgtc gcttggtata atgctggaaa
    13141 aactggagaa atccggagct tttgccaatg ctctctatac ttcgatgaag ggtatggggc
    13201 tcaacctttt cgatcgttca acaaaagatg aaaatggttc gatgacacca tttcaggcta
    13261 tgcttctcga gcagagaaca gaacaatgga ccgggactaa atataggctt tatactattg
    13321 atgatgtgct tgcccaggca aagcagattg tagttgcgat cgataccgga agcacagtga
    13381 ttttgatcgt cttgttcgcc attgtgatga ttggtatatc caatactttc cgaatggcaa
    13441 tgtatgagcg aatccgagaa attggaacaa tgcgcgctat tggtgttcaa cggaatgaaa
    13501 taagatctat gtttctatat gaggcactat tgctttcgct ggcaggtgct attgcgggaa
    13561 tagtcttggc acttatcgcc atgggcttgt tgacgcttat cagttttgat ccgctttcgc
    13621 cagtattctt gattctgaaa cgcggtcatt tttcattcta tgtgccgccg cttcgtgcta
    13681 taggcaatat cctccttatc gcggtgctta cactcgttgc agtatatgca cccgcaaatg
    13741 ctgcggcaaa attgccaccg gcagaagcct tgagaacagt aaaataaata ggagtctatc
    13801 agcatgtatt cgaatgtatc ccggaaggca aaaagcctta ggagaaggat tttatccatc
    13861 ttgtctttat tattgctgtt ttttacggga ttgaatcccc tgctagccca atcaaagccg
    13921 gatcccagag agctcatcaa gcaagtcgat gatctgagcg atttttctgg caaggatttt
    13981 tcagctgttt tcaccattgt cactcaaaaa ccaggggaga aagacagcgt cactcaggcc
    14041 cgagttttcc gcagagattt gaaaaagcaa ttcgtaattc tgatcttgct cccggaagtc
    14101 aacaaaggac agggttatct cagagaagat gataatgtct ggttttatga tccaacaagc
    14161 cgcaagtttt cgcattcttc gatgaaagag aatatacaga acacaaaagc gaagaattct
    14221 gattttactc tcagcacctt tgctgaggat tataccgtga caaaaatgac cgagagcatg
    14281 gtagggaaat ttccggtttg ggtattggat ctaaaagcga gaacaaatga agtagcatat
    14341 gatcgggtag tgctctatat ccgcaaagac aggacaatgt tgctcaaacg agaagactat
    14401 tcggtaaatg ggcgccttat gcggactact gcatatccga aatacgtaga gctggaaggg
    14461 aaactccttc cttcgcagat attgatcgtt gatgaagtca acaaaggaga aaagagccag
    14521 atgaccatga cggaacaatc tgtgtctccc ttgccagata gggtgtttac aaaggctttc
    14581 ctcgaacaag taagcaggta atccaggagg taataaagga ggatgtagcg atgagtcttc
    14641 ttcggaactt aggtgcaatc ggtattctat gtgtgattat tatggctgcg cccttcagca
    14701 tatcatctca agaagagagc aatgatatcg atgcgctctt tgaggatgaa ataatagttc
    14761 agcaagacag cgatactctc ggtcaagaag aatcccaaga ttcattcata ggccagacaa
    14821 atccacttca gagttttcta aaaaccgaga ctgtaaagat cggcggaaac attagcggga
    14881 ctgtgagcgc ttcctggaaa tggaatgatc cgtggacggt gggttttgac ctcgcaaatc
    14941 cagattcaca taaattggcc ccagccttga atagtctctt gtacttcgat gcacggccag
    15001 aggaggattt ccgcgtacat ggctcgttca agaccgcttg gccgtttgca gagacaaata
    15061 gcctcttgga ttccaacaac aaaactacat tagtctctgt cccagatgtt cgcattttcg
    15121 aactgttttc ggatttccag cttggagata atgcttatct gcgtttcgga aaagccacgg
    15181 ttaaatgggg tgtcggatac ttctttagtc cagcggatat aatcaacctg gaatcgataa
    15241 atattttaga tccaacggcg cagcgtgaag
//
LOCUS       DAOOWY010000043        15038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_3988_length_15038_cov_8.721484, whole genome shotgun sequence.
ACCESSION   DAOOWY010000043 DAOOWY010000000
VERSION     DAOOWY010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15038
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_15038_cov_8.721484"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PK105_04820"
     CDS             complement(<1..554)
                     /locus_tag="PK105_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61064.1"
                     /translation="MEEIKGTEALEREILEDARKRAERIIRKAEESARLLGVQTEKKI
                     EEATTALVGEYQAKKRIAELEMLSRLPLEKARLDISYRDEMLRKALKGALESMNPRLF
                     GLWCVKRLACQAELVRNSRARVLVHGLDSETMRDIEALFGQGSDISIEEAPAMKVRGL
                     VVEPMDTSYRISLTENELLEWLLD"
     gene            complement(577..924)
                     /locus_tag="PK105_04825"
     CDS             complement(577..924)
                     /locus_tag="PK105_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOU61065.1"
                     /translation="MAEIFVLAEEELLIGFGMIGIKGAAIFDRDDAITAFRSVVRDKR
                     YSHEGQTFDLSDCKMLILSEDVSDMIGEELLEWQLSGDFPLIVEIPPLAGSSAQHTRL
                     VDAVRKAIGIKIE"
     gene            complement(996..1301)
                     /locus_tag="PK105_04830"
     CDS             complement(996..1301)
                     /locus_tag="PK105_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOU61066.1"
                     /translation="MKKKLLFGVMAFMASAAVFAQTQAEPAAVSSSVQMVKYIAAALA
                     VGIAAIGGAMAVAKIGAAAMGAMAEKPDLSGKALPYVGLAEGICLWGFLVAVLILFL"
     gene            complement(1344..3503)
                     /locus_tag="PK105_04835"
     CDS             complement(1344..3503)
                     /locus_tag="PK105_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOU61067.1"
                     /translation="MSSVSMRKVELLLLKSDIDAVLKYLATKRCFQIIYPDEIERLAR
                     EKRASFGLGGNEKLLASDDAFNKAPTAASTIARPAMNVKRFDESHLEERLINEPDLDE
                     ERLDDAQRKLDFISQFLGIESPKDIIEDSRLPDEDMLSKLEVLFDRCSQLKVSLDEEQ
                     AKLDGLAESIHEAKAFASLSRPFEEFEKFSYVSIQIGKVAKDKIEELSQMLGDRAVII
                     PLDEEGTILAASSRKGRFAFETALSKVGFEKKKPPEGLKGIPSETMNALEGAYQVGKL
                     HFEKLLTEKKALKDQYSDLLQIIASSIRLKKAIALVESKLEKTRWAYRLSGWVPENMV
                     DKLVKDLLTMLGNRISIRVYDPPELDLAGTKEKQEGVPVLLEHNSFVSAFQGIVLSYG
                     TPLYGDIDPTPFVAFFFTLLFAIMFGDLGQGMVIASLGLAMHKAKKGLLASYKKYAHA
                     FIAAGVGSMIMGLLVGSFFTSDTALIPIERALTGFFLGKPADRFLQIMPRENVSAMFY
                     FFGFTLGVGVIINSTGIVINIINLIRRKEYGEAFFSKTGLAGALLFWWAIVMVVRIIL
                     GNKLGIVDVAGIGLPLLAIIFAEPIKSFIDKTYGKPHTEEASNWLIGGIIELLDIVSY
                     FASNTFSFLRVGAFALAHAVLSFVIFTMGSLVSGKSASGLALEIIVFLFGNAVIIVLE
                     GLIVTIQVIRLQYYEFFSKFFTRTGEPFKPVSFAGKV"
     gene            complement(3500..4501)
                     /locus_tag="PK105_04840"
     CDS             complement(3500..4501)
                     /locus_tag="PK105_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOU61068.1"
                     /translation="MAETLDIAYLYARVCGAFSNMRLGDKAGELMQNSGGIVGLWRLL
                     FDEEAPSIAEARLVFEAERRIIKRAIDNFVRLTKPFSDSSEFIRALISKYEISTIKSM
                     LFRLRSGEPKPEATTYFSNIIGQALARWPRITDMFSDTPYSWLDSHWLDKIAMAENEL
                     DKQYYLELWKAALGLPRQKVGAIPELIRWEVIYQNIVWAIRTSRYYGLSKEDIADTLV
                     YLPQEDSTSLALETFSYDLERIGSFDSWRLKKLLSNQTGMVLDVPALEIRVLEDLFFM
                     VRRSLHLYPFSYTPIYCYFKLLDYETSLLLAVLESTRLSVTPEEKIKYVWIPGGGTA"
     gene            complement(4503..4811)
                     /locus_tag="PK105_04845"
     CDS             complement(4503..4811)
                     /locus_tag="PK105_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61069.1"
                     /translation="MDSADSIDRLLEIEQKAAQIIQEAEEKASGLLVEAKKRSEIVQN
                     QKISQMRRELEADFAAYIDSLTQKSHKEIEEYRLSLNSIPLNRKSLLETLEALLQTEN
                     "
     gene            complement(4821..5297)
                     /locus_tag="PK105_04850"
     CDS             complement(4821..5297)
                     /locus_tag="PK105_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU61070.1"
                     /translation="MILQNAFKPATIKIGLESEYKDELLEELVDILAKDYPKDRIFPR
                     EEALRALRKREEKMSTGIYKGVAVPHATFDSIDKLRGVIGLSKNGIDFESLDGNPVYL
                     VFMLVSPPSQAEAHLEALRQIALLIKDHLLLENLMKATTPEKVFALIREFEDSLNP"
     gene            5460..6317
                     /gene="uppP"
                     /locus_tag="PK105_04855"
     CDS             5460..6317
                     /gene="uppP"
                     /locus_tag="PK105_04855"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HOU61071.1"
                     /translation="MLIIQSIILGMVQGLAEFIPISSSAHLIIIPWLFGWNNPALTSL
                     TFDVALHLGTLLAVIVFFASDWARLISAWFKSVIQFKIGEDPDRLMAWYILLACIPGG
                     VSGLLMESKINEAFHSNPIPRSSMLFMAGSIALLALLLLIADKYAQHRRSFNQIKARD
                     ALCIGLAQALAVIPGVSRSGSTITAGLALGLEREAAARFSFLLSAPIIAGAGLKSLYD
                     FSEQIGAGTIGKSELSIFPIGFIVAAVSGFFCIKYLLSYLKKHSAELFVWYRFALAAI
                     VVVVSLIRG"
     gene            6476..7834
                     /locus_tag="PK105_04860"
     CDS             6476..7834
                     /locus_tag="PK105_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOU61072.1"
                     /translation="MRIVLIGAGSAQFGFGTLGDIFSSTLLKGSHIALVDIDGEALGN
                     VYKAAQAFAEANGLNFTLSAHTDRREALKGADFVIISIEVGNRFELWDQDWKIPLQYG
                     IPQVYGENGGAGGLFHALRIVPPILEICQDCAELCPDATIFNFSNPMTSITTTVLRKF
                     PGLKVIGLCHEIASLARYIPPMLGVPLEKLELRAGGLNHFSVLISARYRETGEDAYPE
                     ILAKAPAFFERMIGYSDILEYIQETGNIPKTEGETDLPDIGRTLSSRQWADRKLFRQI
                     LEHFHLLPITSDSHIGEYIPWAHSVADNKGILDFYLLYSSSLSLLQPKIELKVKERLV
                     PIIEGILQDSGYEEAAVNIMNNGLIPSLPGNVTVEVPARVHRKGIEGVAFPDYPRAFA
                     ALLRNYTGVYDLTAEAILQKSKDLVIQALLVNPVVPICEQVPELVDVMISRQAQWLGY
                     LR"
     gene            7949..9355
                     /locus_tag="PK105_04865"
     CDS             7949..9355
                     /locus_tag="PK105_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU61073.1"
                     /translation="MEDVQHESVYVAPAHDAARIEVFRSASIPSAVASLALPTVISQL
                     VTMIYNVTDTFFVGQLGDPRKVAAVSLVMPAYTMLTAIANLFGVGGSSVISRFLGSGQ
                     PAKAKQTSSFCLLSAIFVAAIFSILAFIFQRPLLMLLGANQETYDFASNYIIWVLVIG
                     GIPTVMGMLFGHLVRSEGGAKQASIGMSFGGILNIILDPIFIFTFGLEVKGAAIATLL
                     SNCAVVCYFVVYLAYKKGKTIISFVPRDLNQEFSIATKVVSVGLPASLQNILSLTSNT
                     VLNNLTAKYGSAALAAIGIAKKIDMLPMNVTMGISQGVLPFIGYNYGAKRFDRVHQAN
                     KFARTVAVCFSLLCIIIFEIFAEPIVRLFINDPETIRLGANFLRILCLMTPMMAISYL
                     ITTMFQATGQGKRALTISVFRKTTIDVPLMFLMDKLIPLYGLMTVQPIVDTLSVGLAF
                     ALYRDFSRKLAAQTDQLK"
     gene            9364..10380
                     /locus_tag="PK105_04870"
     CDS             9364..10380
                     /locus_tag="PK105_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU61074.1"
                     /translation="MIQCLLKKKRPAGTERWETTMFSALQSVFSVIIMIAIGFGLSKA
                     RWFEDGGSVLVSRLVVNVALPAYMISNLMGGYDRQTLLSMLPGLVIPFAVMIVSYLMA
                     MMVGKLLKVKEDRKGTFRSMFALSNSVFIGLPVNLLLFGDESLPYALLYYIANTTLFW
                     TIGVYGIAMDGALRNSRPKPSLVSLSGLKRILSPPLIGFLIAVLFILTGIMLPKPIMD
                     TCKYLGNMTTPLSMLFIGIVIARVDWKELKFEKDFIAVLVGRFLITPMFMFMVVRSLD
                     IPLLMKEVFVMQASMPAMTQTPILAETYKSDTEYAAIGTSLTTVASMITIPFYMSIMR
                     VLFD"
     gene            10514..11704
                     /gene="carA"
                     /locus_tag="PK105_04875"
     CDS             10514..11704
                     /gene="carA"
                     /locus_tag="PK105_04875"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOU61075.1"
                     /translation="MSVHFKSACLVLEDGSTFEGYSIGADISAEGEVVFSTGMVGYNQ
                     SLTDPSYCGQILVFAYPLIGNHGVPRMRKNAFGIPIDFESEKIQVSAVVIAEACSEPS
                     HYSAERSFSKWLNDENVPGIAGIDTRRLIRTLREEGVMHGKILIEGTPQPIRDEFKVG
                     NPVRLVSPSSPKRYIPENYQLTDRSSTKKWEKYGSVENSSKMSKRPTIALVDCGAKAN
                     ILRILLDAGSEVIRLPWDYPLDHIEYDGLFVSNGPGDPKACTRTIAHIRHALMESKPI
                     FGICLGTQLLALASGADTYKLKYGHRGQNQPVIETKSRRCYITSQNHGYAICEDSLPT
                     GWEPWFVNGNDETIEGIRASKAPFRAVQFHPEGCPGPRDTQFLIQEFLQEVAVASSKT
                     RYES"
     gene            11701..14970
                     /gene="carB"
                     /locus_tag="PK105_04880"
     CDS             11701..14970
                     /gene="carB"
                     /locus_tag="PK105_04880"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOU61076.1"
                     /translation="MKKVLVLGSGGLKIGQAGEFDYSGSQALKALREENIRTILINPN
                     IATNQTSEGMADTTYFLPLTPEFVTQVIEKERPDGILLSFGGQTALNCGLALCKAGVL
                     YKYAVDVLGTPIEAIELTEDRELFANHLRKLGLKTPESKAAANIVEALTCANTIGYPV
                     MLRAGFALGGAGSGLCKNERELRIRAEEALALSPQLLIEEWLGGWKEIEYEIVRDKTD
                     NCIAICNMENIDPLGIHTGESIVVAPSQTLTDEEFHNLRSIALKLVRSLGIVGECNIQ
                     YALDPRCGDYRIIEVNARLSRSSALASKATGYPLAYVAAKLALGYSLIDLHNRITGVT
                     TAFFEPALDYCVIKMPRWDLAKFKEVDTHIGSEMKSVGEVMAIGRSFEEALQKAIRMT
                     GASQFGLQSDGGRMPIEPKKLRASLSKPTDKRLFAVYQALAEGWSIQKINRLTGIDPW
                     FIEKIDHIRQCEVEMYKSAESACQAQVAPSVPILDDKTIAVLLDAKRLGFSDAGIAHI
                     FGTNELEVRRWRQIMGLRPSVRQIDTLAAEYPAQTNYLYVTYAHSFASPLLASNSVIA
                     SPDDVKPSHGSILVVGSGPYHIGSSVEFDWCAVSSIETCRALGHPTIMVNCNPETVST
                     DYDVCDRLYFEELSFERIMDIYEFEEPSGILLSMGGQVANNLALPLFDAGALILGTSP
                     QNIDKAEDRHKFSSLLDELGIEQPAWSELTTNEEAFAFAESVGYPVLVRPSYVLSGAA
                     MNVAWDDKSLATFLGLAADISQEHPVVISKFIEHAKEIEIDAVAKHGKLLYHAITEHL
                     ENAGVHSGDATVVFPAQRLYVETVRKIIRITEKIAIALNITGPFNIQFVAKQSRVMVI
                     ECNLRASRSFPFCSKVSRVNMIDLATRAMLSQAVETAQNALYDQPWVGVKAAQFSFAR
                     LHGADPVLGVEMTSTGEVGCIGSELNDAFMKAMVSVGYSSKVKKLLLSTGPLEDKLDF
                     LESAQKLVEMGCTLYASHGTAKFLKKYGLEAQALHWPLETAEPNILTLMRSHAFDLVI
                     NIPKNNRKHELQNDYLIRRMAIDLNIPLFTNIKTARQYIESLAYTKIHGLEIKPWEEY
                     R"
BASE COUNT         3968 a         3691 c         3426 g         3953 t
ORIGIN      
        1 tcatctaaga gccattccaa aagctcattt tcggttagcg aaatgcgata gcttgtatcc
       61 atgggctcga ctaccaggcc gcgaaccttc atggctggag cttcctcgat cgagatgtca
      121 gaaccttgcc caaacaatgc ttcaatgtct ctcatcgtct ctgaatccag gccatgtaca
      181 agcactcttg cgcgcgaatt gcgaacaagc tcggcttggc aggcaagcct tttcacacac
      241 cacaggccaa aaagacgagg attcatgctt tcaagtgccc ccttaagcgc cttgcgaagc
      301 atctcgtcgc gatacgaaat atcgagcctt gccttttcga gaggaaggcg cgaaagcatt
      361 tcgagttctg cgatcctctt tttggcttga tactctccta caagagcagt tgtggcttct
      421 tctatttttt tctcggtttg tacaccaagg agtctggcgc tttcctcggc ctttcgaatg
      481 atgcgttctg cgcgtttgcg cgcatcttca aggatttctc gttccagggc ttctgtgcct
      541 ttgatctctt ccatgctttt tcctaattct gctttgctat tcgattttga ttccaatcgc
      601 cttccgtacg gcatcgacga gacgagtatg ttgagccgag gaacctgcga gcggaggaat
      661 ctcaacaatc aaaggaaagt ctccgctcaa ttgccattcc aaaagttctt cgccgatcat
      721 atccgataca tcttcgctaa gtatgagcat cttgcagtcg gagaggtcaa aagtctggcc
      781 ttcgtgcgaa tagcgtttgt cgcgcaccac gcttcgaaat gccgtaattg catcatctcg
      841 atcgaatata gccgctcctt ttatgcctat catgccaaag cctatgagaa gctcttcttc
      901 agctagcaca aaaatctctg ccatagtacc ctctagaata atgctctctg gcatccatga
      961 gccttgccag gggcatcatg ccaacctatt ctcccttata ggaacagaat aagcacggca
     1021 accaagaatc cccacagaca aatgccttcc gcaagaccga cataaggcaa agccttgcct
     1081 gaaaggtctg gcttctctgc catggctccc atcgctgcgg cacctatctt tgcgacagcc
     1141 atggctccac ctatcgccgc tatgccaacc gcgagagcag cggcaatata ctttaccatc
     1201 tgtaccgagg acgatacggc tgcaggttca gcctgggtct gagcaaaaac agcagctgaa
     1261 gccataaagg ccataacacc gaatagcaat ttcttcttca tacaaaatct ccttctaaca
     1321 taatgttata gatcatgact tcattatact ttacctgcaa agctaactgg tttgaatggc
     1381 tcacctgtgc gggtaaagaa tttcgagaaa aactcataat actgaagcct gataacctga
     1441 atagtcacaa ttaaaccttc cagcacaatt atcactgcat tgccaaataa aaatactatg
     1501 atttctaatg ccagcccaga agcggattta ccgctcacaa gactgcccat agtaaatatc
     1561 acaaacgaaa gcacagcatg ggcaagtgca aaggccccaa ccctcaaaaa gctaaatgta
     1621 ttcgaggcaa aatagctgac tatatccaat agttcgataa ttccgccaat tagccaattc
     1681 gaagcttctt ctgtatgtgg ctttccataa gttttgtcga taaaggactt tattggctcg
     1741 gcgaatataa tagccaggag tggtaatcca atgcctgcaa catcgactat acccagcttg
     1801 ttccctaaaa tgatcctaac taccattact attgcccacc aaaacaatag cgcgccggca
     1861 aggccagtct tcgaaaagaa ggcctcacca tattcttttc tgcggatgag gttgattata
     1921 ttgataacaa tcccggttga gttgattata acgcccacgc ccaatgtgaa tccaaagaaa
     1981 taaaacatgg cactcacatt ttctctaggc attatctgca aaaaacgatc ggcaggtttg
     2041 cccaagaaaa aacctgtcaa agctctctcg atcggaatga gagcggtatc gctcgtaaag
     2101 aaagaaccta ccagcagtcc cattatcatc gaaccaacgc cggcagcgat aaaagcatgc
     2161 gcatatttct tgtaactcgc cagaagacct ttctttgcct tgtgcattgc aagccccaga
     2221 cttgcgatca ccataccttg acccaaatcg ccgaacatta tcgcgaacag caaagtgaaa
     2281 aagaaagcaa caaatggggt gggatctatg tctccatata gaggcgtgcc ataggatagg
     2341 acaataccct gaaatgccga tacgaaagaa ttgtgctcta agagaaccgg cactccttct
     2401 tgcttttcct ttgtgccggc aaggtccagc tcaggcggat cgtagacacg aatggagatc
     2461 cggtttccaa gcatagtgag gaggtctttg acaagtttat cgaccatatt ttcaggcacc
     2521 catcccgaaa gtcgataggc ccaacgcgtt ttttcgagct tactctccac caaagcgatg
     2581 gctttcttga gccgaatgga ggaggctata atttgcaata aatcactgta ttgatccttt
     2641 aatgcctttt tttcggtcaa gagtttctca aaatggagct ttcctacctg ataggcacct
     2701 tcgagtgcgt tcattgtctc cgacggaata cccttgagcc cttccggtgg tttctttttt
     2761 tcgaatccca ctttggaaag cgcggtctcg aaagcaaagc ggcccttgcg gctagaagca
     2821 gcaagaatag tcccttcttc gtccagagga attataactg ccctatcgcc aagcatctga
     2881 gaaagctctt cgattttatc ttttgccacc ttgccgatct gaatcgagac ataggaaaat
     2941 ttttcgaatt cttcaaatgg ccgagaaagg ctggcaaaag cctttgcttc atggatggac
     3001 tctgcaagcc catccaattt cgcctgctcc tcatctaagc tcaccttgag ctgcgagcag
     3061 cggtcgaaga gcacttctag ctttgagagc atatcctcat cgggcaaacg gctatcttcg
     3121 ataatatctt ttgggctttc gatgccaagg aactggctta taaaatcaag tttcctttgc
     3181 gcatcatcta gcctttcctc atcgagatca ggctcgttga ttagtcgttc ttcgagatgc
     3241 gactcatcaa agcgcttcac gttcattgca ggcctggcta tcgttgaagc agccgtaggt
     3301 gctttattaa acgcatcgtc actggccaac aacttttcgt tgccccccag tccaaagctc
     3361 gccctctttt cccttgcaag ccgctcgatc tcatcgggat aaatgatctg aaagcaacgc
     3421 ttggttgcaa gatatttcag caccgcatcg atatcggatt tgagaagcaa tagctcgacc
     3481 ttgcgcatgc tcacgctgct catgctgttc ctcctcctgg aatccacaca tattttatct
     3541 tttcctctgg ggtcacactt agcctggtac tctccagtac cgccaagagc agcgatgtct
     3601 catagtccag tagcttgaaa tagcagtaaa tcggcgtata gctaaatgga tagagatgca
     3661 ggcttcgcct aaccataaaa aatagatctt caagaacacg aatctccaaa gcggggacat
     3721 ctagaaccat ccctgtctgg ttggacagta gtttcttaag tcgccatgaa tcgaaacttc
     3781 cgattctctc aagatcataa gaaaaagtct ccagagcaag cgaagtactg tcctcttgcg
     3841 gcaaatagac gagtgtgtcg gcaatatcct ctttcgacaa tccataatag cgcgacgtac
     3901 gaatcgccca cactatattc tgatagatga cctcccatcg tatcagctcc ggaatcgctc
     3961 cgactttctg ccttggcaag ccgagcgccg ccttccacaa ttccagataa tattgcttgt
     4021 cgagctcgtt ttcggccatt gctatcttgt cgagccaatg gctgtcgagc catgaatatg
     4081 gagtatctga gaacatatcc gtaatgcgag gccatcgagc cagggcttgc ccgatgatat
     4141 ttgaaaaata ggtagtcgcc tcaggctttg gttcgcctga acgaagtcta aatagcatcg
     4201 attttatggt gcttatctca tatttcgaaa tcagtgcacg gatgaattcg ctggaatctg
     4261 aaaagggttt tgtaaggcga acaaagttat ctatggcgcg ctttattatt ctacgttcgg
     4321 cctcgaagac aagccttgcc tcggcgatac ttggcgcctc ctcatcaaag aggagtctcc
     4381 atagccctac gatacctcca gagttctgca tgagttctcc ggccttatcg cccagtctca
     4441 tgttcgaaaa agccccacat acgcgagcat agagataggc gatatcgagc gtttcggcca
     4501 tgtcagtttt ccgtttggag aagagcctcc aatgtctcca aaagcgattt ccggtttagg
     4561 ggaatggaat taaggctcaa gcgatactct tcgatctcct tgtggctctt ttgggtaaga
     4621 gaatcgatat aagctgcaaa atctgcctcg agttccctgc gcatttgaga aattttctga
     4681 ttttggacta tttccgaccg tttttttgct tcaactaaca atccggaagc cttctcttcg
     4741 gcttcctgga ttatctgagc tgctttctgc tctatttcga gcaatctatc gatgctatct
     4801 gctgaatcca taacacaacc tcatgggttg agactatctt cgaattctcg aatcagggca
     4861 aacaccttct cgggtgtagt agctttcatg agattttcga gcaagagatg atctttaata
     4921 agaagggcga tctgtcgaag agcctcgaga tgggcttctg cttggctggg aggagaaacg
     4981 agcatgaata ccaggtacac cggatttccg tccagagatt cgaagtctat accattttta
     5041 gataaaccaa tcacgccacg aagcttatcg atgctatcaa atgtcgcatg aggaaccgct
     5101 actcctttat aaataccggt agacatcttc tcttctcttt ttctaagagc tctcaaggct
     5161 tcttctcttg gaaagatccg atctttcgga tagtcctttg caaggatatc taccaattct
     5221 tctagaagtt catctttata ttcgctttcc agcccaatct ttatcgtagc cggcttgaat
     5281 gcgttctgaa gtatcatagc agtttcctat ctcctgattg gcctatctat gggctcagga
     5341 agattagctt taatgatagt gaaagacacc ccggatgaca aggctgtatg ccatattgcg
     5401 gataaacctt aaacagagta aaaattagaa tgctatagcg ttacccaaga ggaaaatgca
     5461 tgctcattat tcagtctata attcttggaa tggtccaggg cctcgccgaa tttattccta
     5521 tatcaagctc cgcgcacctt ataatcattc catggctctt tggatggaac aatcctgctc
     5581 tcacaagcct tacctttgat gtagcgcttc atctcggaac gctactggca gttattgtct
     5641 ttttcgcctc cgattgggcg cggctcatct cggcttggtt caagagcgtg attcaattca
     5701 aaataggcga agatcctgat cgactcatgg cctggtacat cttgctcgct tgtatccccg
     5761 ggggagtctc gggccttttg atggaatcca agataaacga agcttttcat tccaatccta
     5821 taccgcggag ctcgatgcta tttatggctg gatctattgc tctgcttgca ctccttctct
     5881 tgattgcaga taaatacgca cagcatagaa ggagttttaa ccagataaag gcccgcgatg
     5941 ctctctgtat tggccttgca caggctcttg cggtcattcc gggtgtatcg cgctcgggtt
     6001 cgactattac tgcgggactt gcgcttggcc tcgagaggga agcagcggcg cgtttttcct
     6061 ttctgctttc tgcgcctata atcgccggag cagggctaaa aagtctctac gatttttcgg
     6121 aacagatagg tgcaggaacc attggtaaaa gcgaattatc tattttcccg ataggattca
     6181 ttgtggcagc cgtgagtggt tttttttgta tcaaatatct tttgtcctac cttaagaagc
     6241 attcggcgga gctgttcgta tggtatagat tcgcgctcgc ggctatagtg gtagttgtat
     6301 ctctgattcg gggatagtct atttgcagat agtggatcta aagggattct ctcttttgga
     6361 tacttaaatc ggggacggtt ttggttcgcg aataattcat tgagaagtaa agcgatgcac
     6421 gcctcccctt tatatatcga tagtattctg gatcgagatt attggaggaa taacaatgag
     6481 aatcgtactt attggcgctg gaagcgcaca gtttggattt ggcacgctgg gcgacatttt
     6541 ttcgagcact ttgctcaaag gttctcatat tgcgcttgtc gatatcgatg gagaagccct
     6601 tggcaatgtc tataaggccg cacaagcttt tgcagaggct aatggactca attttaccct
     6661 ctcagcgcat acagaccgaa gagaggctct caagggagcg gattttgtca ttatctccat
     6721 agaagtaggt aatcgattcg aactgtggga tcaagattgg aagattccat tacaatatgg
     6781 catacctcag gtctatggtg agaatggcgg cgcaggcgga ctttttcatg cgcttcgaat
     6841 tgttccgcct attctcgaaa tttgtcagga ttgtgcagag ctctgtcccg atgcgacaat
     6901 tttcaacttt tccaatccta tgacttccat caccactaca gttcttcgaa aattcccagg
     6961 gctgaaggtc atcggattat gccatgagat agcctcgctc gctcgctata tccccccaat
     7021 gcttggtgtc cctctcgaaa aactcgagct tagggcaggc ggactcaatc attttagcgt
     7081 ccttatctct gcccgctatc gcgagacagg agaagacgca tatcccgaaa tcctagccaa
     7141 agcgccggcc tttttcgaaa gaatgatcgg ttattcggat atcctggaat acatccagga
     7201 gacaggaaat atacccaaaa ccgaaggtga gactgatttg ccagatattg gtcgtactct
     7261 ttcatcgagg caatgggccg atagaaagct cttcagacaa atattagagc atttccacct
     7321 cctgcctatc acctctgaca gccatattgg agaatatatt ccttgggctc acagtgtcgc
     7381 agacaataag ggaatactcg atttctatct gctgtatagt agctccttga gcttgctcca
     7441 gcccaaaatc gagcttaagg taaaggagcg cttggtgcca attatcgaag gaatactcca
     7501 agactcgggt tatgaggaag cggctgtcaa tataatgaac aatggactca tcccaagctt
     7561 acccggaaat gtaaccgtgg aggtacctgc tcgcgtgcac agaaagggta tagaaggtgt
     7621 ggcatttccc gattatccaa gggcttttgc cgctctcttg cgcaattaca ccggtgtcta
     7681 cgatctcacc gccgaggcga ttctccagaa aagcaaggac ctcgtaattc aggctctttt
     7741 ggtcaatccg gttgtgccta tctgcgagca agtcccggaa cttgtggatg ttatgatttc
     7801 gcgacaggca caatggctgg gttatcttcg atgagatcct tttccgggat ttcataaccc
     7861 ttttagatta tttgtgtatt aggataatct agggttctat gttattcctg ggaatggaaa
     7921 aacgaataag aaaggtggtt cgtgcgattt ggaagatgtt caacatgaaa gcgtatatgt
     7981 agctccagcc catgatgcag caagaataga agtattccgt agtgcttcga tcccatctgc
     8041 agtagctagt cttgccttgc caaccgtaat aagtcaactc gtgacaatga tctacaatgt
     8101 cactgatact ttcttcgttg gtcaattggg cgatccaaga aaggtggctg cggtctcgct
     8161 cgtcatgccc gcatatacaa tgttaacggc tattgccaat ctctttggag tgggtggcag
     8221 tagtgtcata tcgcgattcc ttggatcagg ccaacccgcc aaagccaaac agacatctag
     8281 cttttgcttg ctttccgcaa ttttcgttgc cgccatcttt tcgatactgg catttatatt
     8341 ccagagaccg ctgctcatgc tccttggagc taaccaggag acctatgatt ttgcgagcaa
     8401 ctatataata tgggttctgg tgatcggagg catacctacg gtaatgggta tgctttttgg
     8461 acacctggtg cgctccgaag gaggagcaaa acaggcgagc atcggaatgt cttttggagg
     8521 tatcctcaat ataatcctgg atccgatatt catctttaca tttggcctgg aagtaaaggg
     8581 tgcggctatc gcaactcttt tatcgaactg tgcggttgtt tgttatttcg ttgtatatct
     8641 tgcatacaag aaaggcaaaa caatcatctc ctttgtacca cgggatctga atcaggaatt
     8701 cagcattgca accaaagtgg tctctgtcgg attgccagca tctctacaaa atattctatc
     8761 gcttacttcc aacacagtcc taaataacct tacggcaaag tatggctccg ctgccctggc
     8821 tgcgatcgga attgcaaaaa aaatcgatat gttgccgatg aatgtgacca tgggaatctc
     8881 tcagggtgta cttcctttta taggatataa ttatggagca aagagattcg acagagttca
     8941 ccaggcaaac aagttcgcgc gtaccgtcgc ggtatgcttc tcgcttctct gcattattat
     9001 cttcgagatt tttgcggagc ctattgttag gctctttata aatgatccgg agaccattag
     9061 gctgggcgca aactttctgc gaattctatg tcttatgacg cccatgatgg ccataagcta
     9121 tctcattaca acaatgttcc aagccacagg tcaaggcaaa cgagcactaa ccatctcggt
     9181 attccgcaaa acgactatcg atgtgccgct catgtttctc atggataaac taattccctt
     9241 atatggcctt atgacggtac aaccgattgt tgacacacta tcagtcggcc ttgcatttgc
     9301 gctttaccgg gatttttcga ggaagttggc ggctcagacg gatcagctaa aatagtatgg
     9361 ataatgatac aatgcctctt aaaaaagaag agaccggctg gcacagagag gtgggaaaca
     9421 accatgttta gtgctctcca gagtgtattt tccgtaatca taatgattgc catcgggttt
     9481 gggctttcga aggcccgatg gttcgaagac ggaggaagcg tcctcgtttc tcgccttgta
     9541 gtcaatgtag cgcttcctgc atatatgata tcgaatctta tgggaggcta tgacaggcag
     9601 acgctcctct cgatgctgcc tggccttgtt attccttttg ccgttatgat agtaagctac
     9661 ctcatggcca tgatggttgg taagttgctc aaggtaaaag aggatagaaa aggcacattt
     9721 cgctcgatgt ttgcgctatc gaattcggta tttatagggc taccggtcaa tctattgctt
     9781 tttggagatg aaagccttcc ctatgcccta ctatactata tagccaacac cacgctcttc
     9841 tggactatag gtgtatatgg cattgctatg gatggtgccc tgcgcaatag caggccaaag
     9901 ccttcgctcg tctcgttatc ggggctaaaa cgcatactgt cacctccgct cattggcttt
     9961 ttgattgcgg ttttatttat cttaaccgga ataatgctcc cgaaacccat catggatacc
    10021 tgtaagtatc tgggtaatat gacaacaccc ctttcaatgc tattcattgg catagtgatt
    10081 gcgagggtag actggaaaga gctgaagttc gaaaaggatt tcatagccgt gcttgtaggg
    10141 cgtttcctaa taacgcccat gttcatgttt atggttgtca ggagcctaga tataccactt
    10201 ctcatgaagg aagtgtttgt gatgcaagcc tcgatgcctg ccatgaccca gacacctatc
    10261 cttgcggaaa cctataaatc cgatactgag tacgcagcca ttggaacatc gttgaccacg
    10321 gtcgcttcga tgatcacgat tcccttctat atgagcatta tgagggttct cttcgattga
    10381 gtattgctat gaatctatga tcctctcatt atatatgaat tatatatgaa tatttatcct
    10441 attttgatgc ataaagccct tgaaatattc agaatatttg gataaattcg caaaaagctt
    10501 gcataggagt tatatgtcgg tacatttcaa gtcagcatgc cttgtgctcg aagatggttc
    10561 gacgttcgag ggatattcga tcggagcaga tatctctgca gaaggcgaag ttgtattttc
    10621 aacaggaatg gtcggttaca accagtcttt gacagatcct tcatactgtg gacagattct
    10681 tgtattcgcc tacccactca taggaaacca tggagtccct cgcatgcgca aaaacgcctt
    10741 tggtattcct atcgatttcg agtccgaaaa aatccaggtt agcgccgttg ttatagccga
    10801 ggcttgttcg gaaccgagtc attacagcgc tgagcgatct ttctcaaaat ggctaaatga
    10861 cgagaatgtt cctggaattg caggaatcga cacaagaaga ctgattcgca ctttacgcga
    10921 agagggagtg atgcatggca agatactcat cgaaggaacg ccgcagccca taagagacga
    10981 attcaaggtc ggcaacccag tcagactcgt ctcaccatcg agcccaaaga gatatatacc
    11041 agagaattat cagctcacag atcgctcatc gacaaaaaag tgggaaaaat acggctcagt
    11101 agaaaatagc tcaaagatga gcaaaagacc tacaattgcg ctggtcgatt gcggcgctaa
    11161 ggcaaatatc ctgcgtattc ttctggatgc tggttcggaa gtgatccgcc tgccttggga
    11221 ctatcctcta gaccacatcg aatacgatgg cctttttgtc tcgaatgggc caggagatcc
    11281 aaaggcatgt acaaggacca tcgctcatat caggcatgcc cttatggaat ctaagcctat
    11341 tttcggaata tgccttggaa cacagctcct tgcccttgca tctggcgcag atacatataa
    11401 gctaaaatat ggccaccgcg gtcagaatca gcctgtaatc gagacaaaaa gccgacgctg
    11461 ctatatcaca agccagaacc atggctacgc tatctgcgaa gattctctgc ccacaggatg
    11521 ggaaccatgg ttcgttaatg gaaacgatga aacgatcgaa ggcattcgag cctcaaaagc
    11581 accttttcgc gcagttcaat tccacccaga aggttgccca ggcccccgcg atacccaatt
    11641 cttgatccag gaatttttgc aggaagtagc cgtcgcaagc agcaaaacga ggtatgaatc
    11701 atgaaaaagg tgctggtgct tggctcagga ggactcaaaa tcgggcaagc tggcgaattc
    11761 gattattcgg gatcgcaagc cctcaaggct ctccgcgaag agaacatccg aactatactt
    11821 atcaatccta atatcgccac taatcagaca agcgagggca tggcagatac aacctatttc
    11881 ttgcccttga ctcccgaatt cgtaacgcag gtcatcgaaa aagagcgacc tgatgggata
    11941 ctactctcct ttggtggcca gacggcactc aactgtgggc tcgctctatg caaagccggc
    12001 gttctctata aatatgcggt agatgtactt ggcacgccga ttgaagcaat cgaattgacc
    12061 gaagaccgcg aactctttgc aaaccatctg cggaaactgg gtctcaagac gccagagagc
    12121 aaggcagctg ccaatattgt agaggcgctc acatgtgcca acaccatagg ttaccccgtg
    12181 atgcttaggg cgggctttgc gcttggcggc gcaggttccg gactgtgcaa aaacgaaaga
    12241 gagcttcgca taagagcaga agaagccctt gccctttctc cgcagctgct aatcgaagaa
    12301 tggcttggtg ggtggaagga aatcgaatac gaaattgtgc gagacaaaac tgataactgc
    12361 attgctattt gtaacatgga gaacatcgat ccactgggaa ttcacaccgg cgagagcata
    12421 gtcgtagccc catcgcagac cttgaccgat gaagaattcc acaatttgcg aagcatcgct
    12481 ctcaagcttg ttagaagctt gggtattgta ggtgaatgca acatccaata cgccctggat
    12541 ccgcgatgcg gtgactatcg tataattgaa gtcaacgccc gactttcgcg cagttcggct
    12601 ctcgcctcca aagccaccgg ttacccgctg gcatatgtag cggctaaact agctctcgga
    12661 tattctttga tcgacctgca caataggata acaggtgtca caacggcttt cttcgagccc
    12721 gccttggatt actgtgtgat aaagatgcca cgctgggatc tggcaaaatt caaagaagta
    12781 gatacacaca ttggcagcga gatgaagtcc gtaggagaag tgatggctat aggtcgcagc
    12841 ttcgaagaag cactacaaaa ggccatccgc atgacaggcg ctagccaatt cggtctgcag
    12901 agcgatggcg gtcgaatgcc aatcgaacca aaaaaacttc gcgcttcgct ctcgaagccc
    12961 accgacaaac ggctttttgc agtgtatcaa gctcttgcgg aaggctggag catacaaaaa
    13021 atcaatagac ttacaggtat cgatccatgg ttcattgaaa aaatagacca tatcaggcaa
    13081 tgcgaagtcg agatgtataa gtctgcggag tctgcctgcc aagcacaagt cgcaccgtca
    13141 gtgcccatac tcgacgacaa gaccatcgct gtgctcttag acgcaaagcg ccttggcttt
    13201 tccgatgcag gaattgctca tatttttggt accaacgagc tggaagtgcg acgttggcgc
    13261 cagattatgg gattgcggcc ttcggtgcgt caaattgaca ctctggccgc ggaatacccc
    13321 gctcaaacga attatctcta tgtcacctat gcgcattctt ttgcatcacc gctgttagca
    13381 agcaattcgg ttatcgcttc acccgacgat gtaaagccat cgcatggcag catcctagta
    13441 gtcgggagtg gaccttacca tataggttca agcgttgaat tcgactggtg cgctgtcagc
    13501 tcgattgaaa catgccgcgc tctcggccac cctacgatca tggttaactg caaccccgag
    13561 acagtcagca ccgactacga tgtctgcgat aggctatatt tcgaggagct gagcttcgag
    13621 cgaatcatgg acatctacga attcgaagaa ccatcgggaa tactgctttc catgggcgga
    13681 caggtcgcca ataatctagc cctgcctcta ttcgatgcag gtgcgctcat tctaggcaca
    13741 tccccgcaga acatcgacaa agcagaagac aggcataagt tctcctcgct gctggacgag
    13801 ctaggcatcg aacagcctgc ctggagcgag ctcactacca acgaggaggc attcgccttt
    13861 gcagaatcgg ttggctaccc cgtattagtg cggccgagct atgtgctctc aggcgccgcc
    13921 atgaacgtag cctgggatga caagagcctt gcgaccttcc ttggactagc cgcagacatc
    13981 tcccaggagc acccggtcgt catatccaaa tttatagagc atgccaagga aatagaaatc
    14041 gatgcagtgg cgaagcatgg aaagctgcta taccatgcca taaccgaaca tctcgagaat
    14101 gctggcgtgc attctggcga cgccaccgta gtctttccag cacaacgact ctatgtagaa
    14161 acggtgcgca agatcatccg aataaccgaa aaaatagcaa tcgccctgaa tatcacgggt
    14221 cctttcaaca tccaattcgt cgcaaaacag agccgcgtta tggttatcga atgcaacctg
    14281 agagcgagca gatcctttcc tttctgttcg aaggtctcgc gagtcaacat gatagatctg
    14341 gcaacgcgag ccatgctaag ccaagcggta gaaacggcac agaacgcgct atacgatcag
    14401 ccctgggtgg gagtcaaggc agcacaattc agctttgcgc gccttcatgg agcggatccc
    14461 gttctcggag tggagatgac atcaacagga gaagtgggct gcatcggcag tgaactcaac
    14521 gatgctttta tgaaagccat ggtttcggta ggctattcat ctaaagtaaa aaagctgctc
    14581 ttatctacag gcccgctcga agacaaactc gatttcctgg aaagcgcgca gaaactcgtc
    14641 gagatgggct gcaccctcta tgcatcacat ggcactgcaa agttccttaa aaaatatggc
    14701 ctagaagcac aggcgcttca ctggccactt gaaacagcgg agcccaatat cctcacccta
    14761 atgcgcagcc atgccttcga cctcgtaatc aacatcccaa agaacaatag aaaacatgag
    14821 ctccaaaacg actacctcat ccgcaggatg gccatcgacc tgaacattcc gctttttacg
    14881 aatatcaaga ctgcgcgcca gtacatcgaa tcgcttgcgt atacaaaaat tcatggcctc
    14941 gaaataaagc cctgggaaga atatcgataa atagctgcaa atagttgcct gaaaaatcat
    15001 gagcagattg cgaagaaggt cggctttaga cgaatcgt
//
LOCUS       DAOOWY010000044        14998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4008_length_14998_cov_8.214348, whole genome shotgun sequence.
ACCESSION   DAOOWY010000044 DAOOWY010000000
VERSION     DAOOWY010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 14998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14998
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4008_length_14998_cov_8.214348"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PK105_04885"
     CDS             complement(<1..615)
                     /locus_tag="PK105_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU61077.1"
                     /translation="MPKSLPIKPEDVRKPGMLEIPSIPINQYKRNIKEERKRFGNDGL
                     IAMLHDMIVVREFETMLNTYKTMGSWEGIPYSYKGPAHLSIGQEAAVVGQAAALEPED
                     FIFGSHRSHGEIIAKCLSAARKMDEDKLISIMESWLDGETLSYAKRMPYADAFSLARN
                     FIVFGTLGEIFARKAGFNRGMGGSMHAFFLPFGSMPNNAIVGGSA"
     gene            complement(656..2149)
                     /gene="lpdA"
                     /locus_tag="PK105_04890"
     CDS             complement(656..2149)
                     /gene="lpdA"
                     /locus_tag="PK105_04890"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOU61078.1"
                     /translation="MTDYDVIILGSGPGGYLAAERLAAKKLKVALVEKDDVGGTCLNI
                     GCIPTKTLLNSAKLYAHLRDASSFGIETEGVHINWRQVMAWKEEVSKRLRRSLELTLK
                     KAGVEIIRGVGKLTDGHSVIVFPVNAESDALQPSLYQAQTSQSSDYQSPDSQSSNSAF
                     MIRGQKIIIATGSSPIIPPIRGIKDNPAVLDSTGLLSIGEIPSSLCIIGGGVIGIEFA
                     SLFSAFGSSVTVIEMMDEIVPVMDSQMAVELRKALKNVTFMLGTKVTELEGKKVLFEN
                     KNGEKGSTEADIILLSVGRKANIEDWGAQESGLEIKNRAIATDDRMRTNLPGVWAIGD
                     VTGKSQLAHAAYRMAEVAVSDILASQNSERSNQIFVPETVPWALYSLPEAAGAGLTEQ
                     DARARGYDTHVIRMPYNVSGRFVAENGFSAPGSIKIIIEKSSERLLGIHLLGAYASEQ
                     IWGAALALERRLPLSAFRNMVFPHPTVSEVIREAAWSANPLSGTHIH"
     gene            complement(2228..3535)
                     /locus_tag="PK105_04895"
     CDS             complement(2228..3535)
                     /locus_tag="PK105_04895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOU61079.1"
                     /translation="MAEKIIMPKLGNTVESSVILSWKVKPGDTVTKDTILCEIETDKA
                     TMEVPAGVDGTVLALLKREGDDVPVLEPIAIVGAPEEVWENAADSSQANTASIPISAS
                     TSTVFEQEKEATMLQEANGKEPHSSPRARMAMHNLGVDLGAIDKGNGPGGRILEKDVI
                     EASIDEKSNQEVSMQEPPIQGAFGKILQATQTQTIKEEPPTHEKTEFQSIPLIGIRKR
                     IAGRMHDSVQTTAQYTEHSSADAEHLVALRARLKAQENPNIAHITIGDLVLTAVIKIL
                     PEFPEFNAHLEGNELRLFKPIHLGVAVDTPRGLMVPVIRNAQDLSLLGVSMEVKRLAK
                     ACQSGRADPASLSGSTFTVTNLGAFGVEWFTPIVNTPETAILGVCTIRPTPVKTDKGI
                     ENRMRIGLSLTVDHQVIDGAYAARFLRRLSEIIADIDIMPFSL"
     gene            complement(3569..4324)
                     /locus_tag="PK105_04900"
     CDS             complement(3569..4324)
                     /locus_tag="PK105_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOU61080.1"
                     /translation="MLLDLSEREKSILELLADRNYLSVSELSSTLGVSEVTIRADLSS
                     LENRGYLMRTRGGALPSIHRSILEHQRLHIEEKQRIAKKAAALVCDGDRIMIEAGTTT
                     AFITRYLIGRQDVQIVTNSMLAFSYARINPLLNIILTGGTFRRETESLVGPVAVQNLD
                     NFNARLAFVGTDGFTIQRGMTTQFTEGAEIVRAMNKRAETTWLVADSSKFGKIGFVSV
                     LPLLAVHGIITDGGLPKEALEALQTEGIEVILA"
     gene            4670..6190
                     /gene="gguA"
                     /locus_tag="PK105_04905"
     CDS             4670..6190
                     /gene="gguA"
                     /locus_tag="PK105_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU61081.1"
                     /translation="MATKLLSMEKIDKRFTGVHALRQVDFDLDEGEIHALVGENGAGK
                     STLMKVLVGINPKDSGQIYYMGHLFNPRDPRHALEMGIGIIHQELNMMEHLTVAENIF
                     IGREFTRRNGIILDKREQNKRAEELFTRLKMDIDPTEKVGRLTVGKQQMVEIAKAVSH
                     RLRILILDEPTAALTDAEIEELFAIMRDLKNQGVGMIHISHRLEEIHQIADRVTVMRD
                     GEIVSTKKTNNVTRNQVINMMVGRVIYEQPKVKSMVAPGAPVVLRTHRLNAGKLVHNV
                     SFELRKGEILGFAGLIGAGRTETARAIFGADPIQSGWIEVNGRRVRINSPQDAVAKGI
                     GYLSEDRKRFGLAVNLCLRDNIVMASYDQFTRGLFVDAKKAEDVTEHYIERLRIKTPS
                     VDQLIRNLSGGNQQKVVIAKWLICNCEILIFDEPTRGIDVGAKSEIYAFMNELTREGK
                     SIIMISSELPEILRMSDRIIVMCEGRITGELPIEEANQEAIMHLATMGRQAVLARE"
     gene            6195..7475
                     /locus_tag="PK105_04910"
     CDS             6195..7475
                     /locus_tag="PK105_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61082.1"
                     /translation="MALETVEESRMQRFKERGLKYALAPAALIILYVFFGIFGRNYFS
                     YQTLVNIIDAAYYIGFISMGVTFVIISGGIDLSVGTVMMASTLIGGVALKNGVPMVPS
                     LLIIILVGTAFGFFNGIIVSRLRLPPFIATLGTMMISMGVGSIVSNVQSATFPIRGQP
                     GGWYKNLFLYLSPTGGMIPTGAILLLLVAVICHLILSNTKLGRYTYAIGSNIEAARLS
                     GVDVQKWQMWVYVIAGVTAGIGGISYAAIYTTILPAQGQGFELYAIAAAVIGGTSLSG
                     GVGTIWGTIIGVFIMSVLSTGLPAMNLQAHWQTFFTGVIVIGAVLLDMYRTRKAAEIK
                     IETAADIYKAEMLEKIASMQRELSALKASGDTAKAAELQEKIAATRREMKATYATKKA
                     EEKAELARIRAEERAADHEFTEMLKQTDHILDKK"
     gene            7560..8648
                     /locus_tag="PK105_04915"
     CDS             7560..8648
                     /locus_tag="PK105_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61083.1"
                     /translation="MHKTRRVGTVIVTLILLAAITVPVFAAGPIFIPMISKGFQHQFW
                     QTVMKGAKDAADKYNVVMTFEGPPTEADIQQQVQMLVNAMAKSPSAICLAALDTNSVM
                     DQLNEAMRRKIPIIGFDSGVPNAPKGAIYATAATNSYAAAGLAAQKMFPVIKDKIKAA
                     TPSNPITIIDFNQDATSQSVTDRGKGFRDMMIKLITTEGGRSLSDIKVTGNRAYIAPD
                     SPTSGKAIILDMVVPASPKDTDATATAQSILNRVKSDHIVGIFCSNEGTARALLSASN
                     DGAALPTQYPGLVVIGFDAGKAQKAAVRNRYFFGAITQDPYMIGYYAMELAYKAVMGQ
                     PVADIDTGAKFFDYSNMDQPDIAQLLYD"
     gene            8827..9819
                     /locus_tag="PK105_04920"
     CDS             8827..9819
                     /locus_tag="PK105_04920"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOU61084.1"
                     /translation="MIAACGESLIDMVPSEQGADLFEACPGGCPYNSAIAAARLCVPT
                     WFIGKTSIDFLGDKIIDKLRSSGVDLSMLSRSEQAVTLAFVERDAGGNAKYAFYSEGA
                     ADRFYYPSDLPVTLPESCRFLLVGSISLAQEPSGSSICSLIEREHIARLVSFDPNIRP
                     NLISDKVRYIERFEWICQRSAIVKASDSDLEWLYEMPGRKAAQKVLEMGPELVAFTLG
                     DRGAVLMTKARQVEMPAKSVPVVDTIGAGDSFHAALLVGLGWLKVSTRKDLSALSEEA
                     MQSILRLAISEAALDCTKRGAEPPTLRELALFDPEAVKIAPGIMSSFNTRGVIC"
     gene            9813..10250
                     /gene="fucU"
                     /locus_tag="PK105_04925"
     CDS             9813..10250
                     /gene="fucU"
                     /locus_tag="PK105_04925"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HOU61085.1"
                     /translation="MLIGIKPCISAELLSVLYRMGHGDELVLADAFFTGDSLNSRVVR
                     ADGIRIPDLLDGILRLINLDTYVDDPVVMMLPSKGDLLDPEVEQRYREVIDRYWPKTP
                     PIARMERFAFYERARKAFAVVMTGETVKYGNIILKKGVPPVSD"
     gene            10305..11336
                     /locus_tag="PK105_04930"
     CDS             10305..11336
                     /locus_tag="PK105_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61086.1"
                     /translation="MEQALGSLLSIAVLMVAGYLLAKQGLLPEKAVEGLRLLITNLTL
                     PLVMFRAFLLLHLEGRNLALAVVVFFACAAMGIVGAAIGRLMHAPSPETRFLYQGFEA
                     GMLGYVLFSGFYGAEQLPAFAALDIGQVIYVFTVLMVQMNMAEQARTSRIQELQEKAD
                     MALASKGHGASSQEERCKMKKRSIAGTVFPWRDIVNSKVLWAICLGVVLSIAAPEFAQ
                     RLGEKNVVMNMLFAMVGGLTTPLVCLVIGASLSGGLVLSREVVWVVATRLVVALAMGF
                     GMAHWVVPALGFGVWQRRAALIMFVLPPPFIIPVYYKGNIQFVSSVLTLFTLIAIVTV
                     ALLVLLGVV"
     gene            11336..12343
                     /locus_tag="PK105_04935"
     CDS             11336..12343
                     /locus_tag="PK105_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU61087.1"
                     /translation="MSKATVTIRHVAERAKVSTATVSRVLNNNPCVRQRTRQTVLEAI
                     DSLGYKLNVVARSLKTSRTHTIGVLAPDLSGDFFMFIAESMNRELARRGYSLVVGTSQ
                     SSEEEEAKQVRHLAERLVDGIVLIPVGGSNAYLPQISDFNIPIVFVDRILKGYRADKV
                     LVDNEEGACKAVSALIGEGHHRIGYLGGNPEVTTAEERYKGYCRAMKEAGLEIEQKYV
                     RFGNPTLPFGYRAMEDIIKDPYSPNTWFIVNIFTHLGATNYLISEGEERTRQITFASF
                     DEMPYSPLLRYCRYAVQQPIAEMGKAAANLIIDRIEGIGPPEPQTLRFKTRLIQHKLV
                     R"
     gene            12369..14183
                     /locus_tag="PK105_04940"
     CDS             12369..14183
                     /locus_tag="PK105_04940"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOU61088.1"
                     /translation="MEQSKDFFLQPPKNRWKESPPKIGIRPAIDGRRRGVRESLEEVT
                     MGMAKAAARLISENIRHPTGEPVECVIADTTIGGVAEAAACAEKFAREGVAVTLTVSP
                     CWCYGSETMDMDPLTIKGVWGFNGTDRPGAVYLAAVLAAHSQMGLPAFGIYGRNVQDI
                     SDAQRIPEDVAEKILRFARAGIAAAWMRGKSYLSVGSVAMGIAGSIVNPDFFREYLGM
                     RNEYVDMSEIVRRIEEKIYSEAEFRRAMQWVKTYCKEGQDNNPLDEQHSRVRKDRVWE
                     TCVKMAMIVRDLMVGNPDLRRKGREEEALGHNAILAGFQGQRQWTDHFPNGDFLETIL
                     NSSFDWNGIREPYLVATENDSLNGAAMLFGHLLTGSAQIFSDVRTYWSPEAIRRVTGW
                     QPTGLAEEGIIHLINSGATCLDGTGCQRINGQPAIKPWWQVEPEEAKACLEATQWRYA
                     SLGYFRGGGYSSDFLTEGGMPITMTRINIIKGIGPIIQIAEGHTVTLPQEVHDTLDLR
                     TDPTWPTTWFVPRLTGIGPFKDVYSVMANWGANHGASCYGHIGADLITLASMLRIPVA
                     MHNVPSEKIFRPSFWNAFGMDPEGADYRACSALGPLYR"
     gene            14477..>14998
                     /locus_tag="PK105_04945"
     CDS             14477..>14998
                     /locus_tag="PK105_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOU61089.1"
                     /translation="MQKKLNSNDYLSTIMHYLSRYRKLALPGVGVMIAIAGGQLAGPY
                     ILKQIIDVAVPKGDTSLLLAYGAAFLVIVSVTGALSYLGMILLARLGLSIVTHIKQDI
                     FLHLLKLPVSYFDKHPVGELMSRTETDTERVRDMFSTLGANLIVNVLTMIGIFLVTFA
                     LMPALALIMVGISL"
BASE COUNT         3770 a         3452 c         3867 g         3909 t
ORIGIN      
        1 tgcggagcct cctacgatag cgttgttagg catggagcca aagggcaaaa agaaggcatg
       61 catcgatccg cccatacctc ggttaaagcc ggctttgcgt gcaaagatct ctccaagcgt
      121 tccaaaaacg ataaaattgc gtgcgagtga gaatgcatct gcataaggca tgcgtttagc
      181 ataagagagt gtttctccat ccagccacga ttccattata gatattagct tatcctcatc
      241 catcttgcgg gctgccgaga ggcattttgc gataatctct ccatggcttc tatgggaacc
      301 aaagataaag tcttccggtt caagtgctgc cgcttgtcca actacagccg cctcttgacc
      361 aatggaaaga tgggctggac ccttgtagct gtatggaatc ccctcccacg aacccatggt
      421 cttataagta tttagcattg tctcaaattc tcgcaccacg atcatgtcat ggagcatggc
      481 aatgaggcca tcattcccaa agcgctttcg ttcttccttg atatttcgct tgtactgatt
      541 gatcggtata gaagggattt cgagcatccc aggtttcctg acgtcttcag gtttgatggg
      601 caatgatttt ggcatattat gactccttat gggctcattc cgagccaatt ttcagttagt
      661 gtatatgtgt tccgctgagc ggatttgcgc tccaagctgc ctcgcggatg acttccgaga
      721 cggttggatg cgggaatacc atgttccgaa aagcagaaag cggtagtctt cgttcgagcg
      781 ctagcgccgc tccccaaatc tgctcggaag cgtaggctcc cagcagatga attccaagaa
      841 gcctttcgct tgatttctct atgataatct ttatagatcc aggagcagaa aaaccatttt
      901 cggctacaaa tcggccagag acgttatatg gcattcgaat gacatgggta tcatatcctc
      961 ttgcgcgagc atcctgttcg gttagcccgg cacctgcagc ttcaggcagc gagtaaagag
     1021 cccatggcac tgtttccggc acgaagatct gattgctgcg ctccgaattt tgacttgcaa
     1081 ggatgtcaga gacagccact tccgccatgc ggtaagccgc atgggctaac tggctctttc
     1141 ctgtaacgtc gccaatcgcc cagactcctg gtagattagt tcgcatcctg tcgtctgtgg
     1201 ctatagcgcg attcttaatc tcgagtcctg attcctgagc accccaatcc tctatattgg
     1261 ctttgcgtcc aacggagagc aaaatgatat cagcttctgt agagcctttt tcgccatttt
     1321 tgttctcaaa gagtaccttt ttgccctcga gctccgttac ctttgtgcca agcatgaaag
     1381 tcacattttt tagtgctttt ctcagctcga cagccatctg actatccatt accggcacga
     1441 tttcgtccat catttcgatg acggttacac tcgagccaaa cgcagagaac aagctggcga
     1501 actcaattcc tattacgcct ccaccaatta tgcatagact tgatggaatt tcgccaattg
     1561 ataacaaccc tgttgaatcg agtacagccg gattatcttt tatgcctcta atcggaggaa
     1621 ttatcggaga agagcctgtt gcaatgatga ttttctggcc tctgatcatg aaggcagaat
     1681 ttgaggattg ggaatctgga gattgataat ctgaggattg agaagtctgt gcttgatata
     1741 aagagggttg aagcgcatcg ctttctgcgt taaccggaaa gacgataaca gaatgaccat
     1801 ctgtcaactt tccaactcca cggatgatct ctacgccggc tttcttgagg gtcaattcca
     1861 aggatcttcg cagtctcttc gagacttctt ctttccaggc catgacctgc cgccagttta
     1921 tatgtacacc ttcggtttct ataccaaatg aagaagcgtc tctcaaatga gcatatagtt
     1981 tggcgctatt gaggagagtc ttggtgggaa tacaacctat attgaggcaa gtcccgccta
     2041 catcgtcttt ttcaacaagt gcgactttga gtttttttgc cgcaaggcgt tccgcagcaa
     2101 gatagccccc ggggcccgat ccgaggatga tcacatcata atcggtcata cattttctcc
     2161 atagaagtca tgcatatatt aaaagctata ttcgaagcga catacaaaac tatattcaaa
     2221 gcgatattca aagagaaaag ggcataatat cgatgtctgc aatgatctcg gaaagccgtc
     2281 ttagaaaacg cgcggcataa gccccatcta ttacctgatg atctacggtg agtgaaaggc
     2341 ctattctcat tcgattctca atgcccttat cggtttttac cggagtgggt cggatcgtac
     2401 atacgcccag tattgcggtc tccggagtat ttacaatagg agtgaaccac tccacaccga
     2461 acgcacccag attcgtaaca gtgaaggtag aaccagaaag agaggcggga tctgcgcggc
     2521 cgctttggca ggcttttgcc aggcgcttca cttccatgga aacgccgaga agggagagat
     2581 cttgagcatt tcgaatcacc ggaaccataa ggcctcgcgg tgtatcgacc gcaacaccaa
     2641 ggtggattgg cttgaaaagt cttagctcat ttccttcaag gtgcgcattg aattcgggaa
     2701 actcagggag aatcttgata acggccgtaa gaacaagatc tccaatagtg atgtgtgcaa
     2761 tatttggatt ctcttgcgct ttgagacgcg ctctcagtgc aacaaggtgc tccgcatctg
     2821 cagaactgtg ctcggtatac tgggcggtgg tttggaccga atcgtgcata cggcccgcaa
     2881 tgcgctttcg tatgccaata agggggatgc tctgaaattc ggttttttca tgagttggtg
     2941 gttcttcttt tatagtctgc gtctgtgttg cttgcagtat tttgccaaaa gctccctgga
     3001 tagggggttc ttgcatagag acttcttggt tggatttctc gtcaatcgag gcctcgataa
     3061 catctttttc aaggattctt ccaccaggac cattcccttt atcgatagcc cctagatcca
     3121 caccaaggtt gtgcatagcc attcgggcgc gtgggctcga atgaggctct ttgccattag
     3181 cctcttgtag cattgtggct tccttttctt gctcaaagac agtggaagtg cttgcagaaa
     3241 taggaatcga tgccgtattt gcctgggagg agtctgcggc attctcccac acttcctcag
     3301 gcgcaccaac tatcgcaata ggctccaaaa ccggcacatc atcgccttct cttttaagaa
     3361 gcgctagcac tgtaccatca actcccgcag gaacttccat tgtcgccttg tcggtttcga
     3421 tttcgcatag gatagtgtcc ttcgtaacag tgtctcccgg ctttactttc catgatagaa
     3481 taacgctcga ttcaactgtg ttgccgagtt tcggcatgat tatcttttcc gccattttgc
     3541 tatccctctc tttaattctt ctatttaatt atgccaaaat tacttctatg ccttctgtct
     3601 ggagcgcttc cagcgcttcc tttggcaagc caccgtctgt aatgatccca tgcaccgcta
     3661 acaagggcaa tacacttaca aaaccaatct tgccaaattt ggaggaatcg gcgacgagcc
     3721 atgttgtttc cgctcttttg ttcattgccc ggacaatttc tgctccttct gtgaattgag
     3781 tagtcattcc tcgctggatc gtaaagccat cggttcctac aaaagctaga cgagcattga
     3841 aattatccag gttttgtact gctacaggtc ctacaagaga ctccgtttcg cgtcgaaatg
     3901 tgccccctgt tagtatgata ttcagaagag gatttatgcg cgcataagag aaggccagca
     3961 tggaattagt tacgatttgt acatcctgtc gaccaatgag gtatcgggtt ataaaagcgg
     4021 tggtagtgcc ggcttcgatc ataatgcggt cgccatcgca gacgagtgca gcggcctttt
     4081 tggcgatacg ctgtttttct tctatatgaa gtctttgatg ttcgaggatg ctccgatgta
     4141 tcgaaggaag tgcgcctcct cgagttctca taaggtaacc tcggttttca agagaagata
     4201 ggtctgcacg tatagtgact tccgaaacac ccagagtgct tgaaagctcg gagacagaaa
     4261 gataattgcg atcagccaga agttccaaaa tcgacttctc tcgctcgcta agatctagaa
     4321 gcatttcaaa cctccggaaa acttaaaaat gcctttctaa tatctttcga taatatttcg
     4381 taaagctttc ttttaatata tagccagtct ctaaaacatg caagaccctt aattgaataa
     4441 atgatgtaat cgattgcatt ttatcgcttg acagaataca aaaaccatgt aatcgtttac
     4501 ttatcgctat tgagtcggaa agagcgccta gcaggcaggg cacatcgata gattttatgg
     4561 gttgcaaggc tgagtgcacg gttgtgcacg gctgtgcatg atttagacga gaatatggcg
     4621 atacgtgtat gacctttaag acctaaagca ggggaaagga gtcgcagcgg tggccactaa
     4681 actattgtcg atggagaaaa tcgataagcg ctttacaggc gtacatgcgc ttcgccaggt
     4741 ggacttcgat ctcgatgaag gggagataca cgcgctcgtc ggggagaatg gtgcaggcaa
     4801 atctaccctt atgaaagttc ttgtcggcat taatccaaag gactcaggtc aaatctacta
     4861 catggggcat cttttcaatc cgcgcgatcc aaggcatgcg ctcgaaatgg gcatcgggat
     4921 tatccatcag gaactaaaca tgatggagca tctcaccgtt gctgaaaaca tctttatcgg
     4981 aagagagttc actcggcgga atggcatcat cctggataag cgcgaacaga ataagagagc
     5041 cgaggagctc ttcactcggc tcaaaatgga catcgatcca acagaaaaag tagggcgtct
     5101 cacggttgga aaacagcaga tggtagaaat cgccaaggca gtgtcccatc gcttgagaat
     5161 actcattctc gatgagccca ctgcggcgct caccgatgca gagatcgaag agctctttgc
     5221 aatcatgcga gacctcaaga accagggcgt tgggatgatc catatttccc atcgtttgga
     5281 ggagattcat cagattgccg atcgcgtaac cgtgatgaga gatggcgaaa ttgttagtac
     5341 aaaaaagacg aacaatgtca caaggaatca ggtcatcaat atgatggtgg ggcgcgtgat
     5401 ctatgagcag ccaaaagtca agagcatggt tgcgccaggg gctcctgtcg tgttgcgcac
     5461 acataggctc aatgcaggca aactggtgca taatgtgagc tttgagctcc gaaagggtga
     5521 aattcttggc tttgcagggc ttatcggagc aggccgcacc gaaaccgcac gcgctatttt
     5581 cggcgctgat cctattcaga gtggttggat cgaagtcaac ggcagaagag tcaggatcaa
     5641 ttcaccccaa gatgcggtgg cgaagggcat tgggtatctt tccgaagatc gtaagcgctt
     5701 tggtcttgcg gtcaatcttt gccttagaga caacatagta atggcatcct acgatcaatt
     5761 tacacgtggt ctattcgtcg atgctaaaaa agcggaggat gtgaccgagc actacatcga
     5821 aaggcttcga attaagacac cttctgtcga ccagctcatt cgaaacctat cgggcggcaa
     5881 ccagcagaaa gtcgttattg caaaatggct catttgcaac tgtgaaattc tcatttttga
     5941 tgaacccacg cgcggaatcg acgtaggagc aaagagcgaa atctatgctt tcatgaacga
     6001 gcttacaaga gaagggaagt cgataatcat gatctcttcg gagttgccgg agatcctgcg
     6061 catgagcgat agaatcatcg ttatgtgcga aggacgcata accggagagt tgcccataga
     6121 agaggctaac caggaggcta ttatgcatct tgccacaatg ggtcgccaag ctgtccttgc
     6181 aagggagtga acacatggct ttagagactg tggaagagag ccgcatgcag cggttcaagg
     6241 aacgaggttt gaaatatgcg cttgccccag ccgcattgat aattctgtat gtgttctttg
     6301 gtattttcgg aagaaactac ttctcgtatc agaccttggt aaacatcatc gatgctgcct
     6361 attacattgg attcatctct atgggagtga catttgtcat aatttctggt ggcatagatc
     6421 tctctgtagg aacggtgatg atggcatcca cgctcattgg tggagttgcg ctcaagaatg
     6481 gcgtgcctat ggtcccatcg ctgcttatta tcattttagt gggcactgca tttggattct
     6541 tcaatgggat cattgtctct agactaaggt tgccgccttt cattgcaacc ctcggtacga
     6601 tgatgatctc tatgggcgtg ggttctatcg tttcgaatgt ccagagcgct acgttcccaa
     6661 ttcgtgggca gcccggcggt tggtacaaga acctttttct ttatttgtca ccgacaggcg
     6721 gcatgatacc aacaggtgct attcttttgc ttcttgttgc tgtgatttgc catctcattt
     6781 tgtcaaacac aaaacttggt cgctatacct atgcaatagg tagcaacata gaagcagccc
     6841 gactttctgg cgtagatgtg cagaagtggc agatgtgggt ttatgtcatt gcgggggtaa
     6901 ctgcaggaat tggtggcatt tcctacgcag caatctatac gactattctc cctgcgcaag
     6961 ggcagggttt tgagctttat gctattgccg cggcagttat cggtggtacc tcgctttccg
     7021 gaggcgtcgg tactatttgg gggaccataa tcggcgtatt catcatgtct gtgctttcga
     7081 cgggcttgcc agctatgaat cttcaagccc attggcagac cttcttcact ggcgtgattg
     7141 ttataggtgc tgtgctcctc gatatgtatc gaacaaggaa ggcggcggag ataaagattg
     7201 aaactgcggc cgatatctat aaggcagaga tgctggagaa gatcgcctct atgcagcgcg
     7261 agctttctgc attgaaagcg tctggcgaca cagcaaaggc cgctgaactt caagagaaaa
     7321 tcgcagctac ccgacgcgaa atgaaagcaa cctatgcgac aaagaaagca gaagaaaaag
     7381 ctgagctcgc caggattcgt gccgaagagc gggctgcgga ccatgaattc acagagatgc
     7441 tcaagcaaac ggatcatata ttggataaga aatagaatcg caatggagtc cgcgttgcgg
     7501 gcttaattgg cagataagct tcggagacaa tccggagcat taatgtagag gaggtaggta
     7561 tgcataagac aagaagagtt ggaacagtta ttgtgacgct cattctcctg gctgcaatta
     7621 ctgttcctgt gtttgctgca ggcccgattt ttatccccat gatctccaag ggctttcagc
     7681 atcagttctg gcagacggtc atgaagggag cgaaagacgc cgccgataaa tataatgtgg
     7741 taatgacctt cgaaggacca ccgacagaag ctgatatcca gcagcaggtt cagatgctcg
     7801 tcaatgcgat ggccaagagc ccatccgcca tctgcctagc agctctggac accaattccg
     7861 ttatggatca gctcaatgaa gcgatgaggc gcaaaatccc gatcattgga tttgactcgg
     7921 gcgtacccaa tgcgcccaag ggagccatct atgcaaccgc tgctacaaac agctatgctg
     7981 ctgcagggct tgccgctcag aagatgttcc ctgtcatcaa ggacaaaata aaagcagcaa
     8041 ctccgtcgaa ccccatcacg atcatcgact tcaaccagga tgcgaccagc cagtccgtga
     8101 ctgaccgtgg caaaggattc cgcgacatga tgatcaagct catcaccacc gaaggtgggc
     8161 gctcgctcag cgacatcaag gtgactggca accgggcata cattgcaccc gacagcccca
     8221 ccagcgggaa agcgataatc ctggacatgg tagttcctgc ttcgccaaaa gacaccgatg
     8281 caacggccac tgctcaatcg atcctcaata gagtaaagag cgaccacatt gtggggattt
     8341 tctgctcgaa cgaaggaacg gctcgcgcct tactttctgc atccaatgat ggcgcagcgc
     8401 tgccaactca gtatccaggc ctggtggtga tcggattcga tgctggcaaa gcgcagaaag
     8461 ccgcggttcg caatcggtat ttctttggag cgatcacgca ggatccctac atgatcggct
     8521 attatgctat ggaactcgcc tataaggcgg tgatgggcca gcctgttgcg gatatcgata
     8581 ctggtgcgaa atttttcgac tacagcaaca tggaccagcc agacatagcg cagcttttgt
     8641 atgactgaaa aatcttctga gttctaagaa gatctgtctg gtttaagggt attacatggc
     8701 gtagaaaggg gctgctcatt cttttttggg cagccccttt tgcctaataa ttgaatcaat
     8761 tagtaagagt taagaggaag agaaaaggca gggtatgtgt ctgcttttct tgaggagcaa
     8821 cggagtatga tagcagcgtg tggcgaaagt ctgatcgata tggtgccctc ggagcaaggc
     8881 gcagatcttt ttgaagcatg ccccggtggc tgcccatata atagcgctat tgcggctgcg
     8941 cgcctctgtg taccaacctg gtttatcggc aagacttcga tagacttttt gggagataaa
     9001 ataatcgaca agctgagaag ttcgggtgta gatttgtcga tgctttcacg tagcgagcag
     9061 gcggttacac ttgcatttgt cgagcgagat gctgggggta atgcaaaata tgcgttctat
     9121 tcggaaggcg ctgccgatcg tttttattac ccgagcgatt tgcctgtaac gcttcctgaa
     9181 tcatgccgat ttttgctagt tggctccatt tctcttgcgc aggagccttc cggctcgagc
     9241 atctgctcgc ttattgaaag agaacatatt gcgaggcttg tgagcttcga tccgaatatt
     9301 aggcctaacc ttatttccga taaggttcgc tatattgaga gatttgagtg gatatgccaa
     9361 agaagcgcta tagtcaaagc gagcgactca gatctcgaat ggctctatga gatgccgggg
     9421 cgcaaggctg ctcaaaaagt gctggagatg ggccctgagc tcgttgcgtt cactttgggc
     9481 gatcgcggtg cagtccttat gactaaggcg agacaggtcg agatgccagc aaaatctgtg
     9541 cctgttgtcg atacgatcgg tgcaggtgac agctttcatg ccgcgttgct tgtgggcttg
     9601 ggttggctca aagtcagcac tcggaaggat ctgagtgcac tcagcgaaga ggccatgcaa
     9661 tcgatattgc gccttgccat ttcggaagca gcgctggatt gtactaagcg cggtgcagaa
     9721 ccacctacgc ttcgcgagct cgctttgttc gatcctgaag cagtgaaaat tgctcccgga
     9781 atcatgtcga gctttaacac gaggggagtc atatgctgat tggaatcaag ccttgtatct
     9841 ccgcagaatt attatcggtg ctctatagga tggggcatgg cgacgaatta gtcctcgccg
     9901 atgcattttt tactggagat tcgctcaact cgcgcgtggt acgagctgac ggaattcgga
     9961 ttccagattt gctcgatgga attctgcgtc ttatcaacct ggatacatac gtcgatgacc
    10021 ctgtggtgat gatgcttcca tcaaaaggtg atttactcga cccagaagtg gaacagcgct
    10081 atcgggaggt gatcgatcgc tactggccta agacgccacc catcgcgcga atggagcgat
    10141 ttgccttcta cgaaagagcg cgcaaggcct ttgctgtcgt catgacagga gagacagtca
    10201 aatatggcaa tatcatcctt aaaaaaggag tgccaccagt ctccgattga gagcttgtgt
    10261 ataacagtct ctaaagaaca atctccgaat gaggattgat aaagatggaa caggcgcttg
    10321 gttcgctact ttcaattgct gtgctcatgg ttgcaggtta tctacttgca aagcaggggc
    10381 tgttaccaga aaaagcagtg gaaggtttgc ggctactcat cactaatctc accctgccgc
    10441 ttgtgatgtt tcgagctttt ctattgctac atctggaggg aaggaatctt gcactggccg
    10501 ttgtagtatt ttttgcatgt gcggcgatgg gaatagtcgg tgcagcgata gggcgcctta
    10561 tgcatgcgcc aagcccggag acgaggtttc tttaccaggg atttgaagcg gggatgcttg
    10621 gctatgtgct gtttagcggc ttttatggag ccgagcaatt gcctgcattt gcggcactcg
    10681 atatagggca ggtaatctat gtgttcaccg tgctcatggt ccagatgaat atggcagagc
    10741 aagcaaggac aagccgaatc caggaattac aagaaaaggc tgatatggcc cttgcttcga
    10801 aaggacatgg cgcatctagc caagaagagc gctgtaagat gaaaaagagg agcatcgcag
    10861 ggactgtctt tccatggcga gatatcgtga actcaaaggt gctatgggct atttgccttg
    10921 gtgtggtgct ttcgattgca gcgccagaat tcgcacagag gctcggggaa aagaatgtag
    10981 tgatgaacat gctttttgca atggtagggg ggcttaccac accactcgtg tgtcttgtaa
    11041 ttggcgcatc gctcagcgga gggcttgtgc ttagccgcga ggtggtttgg gtggtagcga
    11101 cgcgccttgt tgtagcgctg gctatggggt ttggaatggc acattgggtt gtgccggcgc
    11161 tggggtttgg ggtgtggcag agacgcgcgg ctttgattat gtttgtgcta ccgccgccct
    11221 ttataattcc cgtatattat aagggaaata ttcagtttgt aagcagcgta ctcacgcttt
    11281 ttacgcttat agcgattgtg actgtggcac ttttggtgct gcttggagtc gtataatgag
    11341 taaggctacc gtgacaattc ggcatgttgc agagagagcg aaggtttcga ctgcaacggt
    11401 gtcgagagtg ctcaacaata atccttgtgt gcgtcaaagg acgcgccaga cagtgcttga
    11461 agcgatagac tcacttggct ataaactcaa tgttgtcgcg cgtagcctca agacaagtcg
    11521 cactcacact attggagtgc ttgcgcccga cctctctggc gattttttca tgtttatcgc
    11581 agagagcatg aacagggagc ttgcgaggcg gggatattct ctggttgttg gcaccagtca
    11641 aagttcagaa gaggaagaag caaagcaggt gcgtcatctc gcggagcggc tagtcgatgg
    11701 aatagtactt ataccagttg ggggttccaa cgcttatctt cctcagattt cagatttcaa
    11761 tattccgatt gtctttgtag accgaatact caaaggctac agagcagata aggtgctggt
    11821 cgacaatgaa gagggagcat gcaaggcggt cagtgctctg ataggagaag ggcatcatcg
    11881 cattgggtat ttggggggaa atcccgaagt cactacggca gaagagcgct ataaaggcta
    11941 ctgcagggct atgaaggaag ccggactgga aatcgaacag aaatatgtgc gctttggaaa
    12001 tccgacgctt ccttttggtt atcgggctat ggaagacata ataaaagacc cctattcgcc
    12061 taacacctgg tttattgtca acatttttac ccatctaggc gcaaccaatt acttgatttc
    12121 tgagggagaa gagaggacta gacagatcac ctttgcatct ttcgatgaga tgccatattc
    12181 acccctattg cgctattgtc ggtatgcagt tcagcaaccg attgcggaga tgggaaaagc
    12241 ggcagccaat ctcatcatcg atagaatcga aggcatagga ccacctgaac cccagacgct
    12301 ccggtttaag acaagattga ttcagcataa acttgtgcgc taaacaacat attcgttgga
    12361 ggaactgtat ggaacagtca aaagattttt ttcttcagcc acccaagaat aggtggaagg
    12421 agagtccacc aaagataggt attcgccccg ccattgatgg ccgtcgcagg ggtgttcgcg
    12481 agtcactgga agaggtgacg atgggcatgg ccaaggcagc ggcgagacta atctctgaga
    12541 atattcgcca tcctactggt gagcctgttg aatgcgtaat cgcagatact acgatcggag
    12601 gcgttgccga agcagccgct tgtgctgaga aatttgccag agaaggagtg gccgttaccc
    12661 ttacagttag cccttgttgg tgctatggct ctgagaccat ggacatggat cctctgacaa
    12721 tcaagggagt gtggggattc aacggcacgg atcggccagg agcggtatat cttgcggcgg
    12781 tattggcagc tcattctcag atgggcttgc cagcttttgg catctatggt cggaatgtcc
    12841 aggacatttc ggatgctcaa agaattccag aggatgttgc agagaagatt ctgcgcttcg
    12901 ctcgcgcggg tatcgctgct gcatggatgc gtggaaagag ctacctctcc gtaggctccg
    12961 tagccatggg tatagcaggc tcgatcgtca accccgattt ctttcgagaa taccttggca
    13021 tgcgcaacga atatgtcgat atgagcgaaa ttgtgcggcg tatcgaagaa aaaatctatt
    13081 cggaagcaga atttcgtcgg gcaatgcaat gggtaaagac ctattgcaaa gaaggtcaag
    13141 acaacaatcc tctcgatgaa cagcattctc gagtccgcaa agacagggta tgggaaacct
    13201 gcgtcaaaat ggcgatgatc gtgcgcgacc tcatggttgg caatccagat ctccgcagaa
    13261 agggaagaga agaggaagct ctcggtcata atgcgattct agcgggcttc caagggcagc
    13321 gccaatggac cgatcacttc cccaacgggg attttctcga gacaatactc aattctagtt
    13381 tcgattggaa tggaattcgc gaaccttatc tggtcgccac cgagaacgat tctctcaatg
    13441 gagctgcgat gctgtttggc catcttttga ccggcagtgc tcagattttc tcagatgtgc
    13501 gaacgtattg gagccccgaa gcaatacggc gtgttacggg ttggcagccc actggtctgg
    13561 cagaggaagg cattatacac ctcatcaatt ccggcgcaac ctgtctcgat ggcaccggct
    13621 gtcaacgaat caatggtcag cccgcaataa aaccatggtg gcaagtagag ccggaagaag
    13681 caaaggcatg tctcgaagcg acacagtggc gctatgcgag ccttggatat ttccgaggag
    13741 gcggatattc ttcagacttt ttgaccgaag gcggaatgcc tatcaccatg actcgcatca
    13801 atattatcaa gggtattggc cctataatcc aaattgcaga aggccacacc gtaacgctac
    13861 cgcaagaagt ccatgatacc ctggacttgc gcaccgatcc tacctggcct accacatggt
    13921 ttgtcccaag gcttacagga attggtcctt tcaaggatgt ctattcggtc atggcgaact
    13981 ggggagccaa ccatggcgcg agctgttatg gtcatatcgg tgcggatctc attactctcg
    14041 cttcgatgtt gcgcattccg gttgccatgc acaatgttcc gtccgaaaag atattcagac
    14101 caagcttctg gaacgccttt ggtatggatc ccgaaggggc tgactatcgt gcctgctcgg
    14161 cgctcgggcc tttgtataga tagactaatg aagagcattc cattcttcta ctagctatca
    14221 gtattatgaa ggtgtaaggc attttataag ggaaaaaact aatacaagat gccgggtcta
    14281 ctagtcgtgg tcccggcacc tttcttgtca attcgttcaa atatcgattc gattgcctgg
    14341 aagcccatct tgagctaggt tatgattgaa gtatacaatt aagcgttttc gagctgtcct
    14401 gaaatatgag ctacggcttt gactcatcgg ggctatccga aagcaagaga ccctctgaag
    14461 gttgctaatt cattatatgc aaaaaaaatt gaattcaaat gattatctaa gcaccattat
    14521 gcattacctg tcgcgctata gaaagctagc gcttcctggc gttggcgtca tgatcgccat
    14581 tgcgggaggg cagctcgctg gaccatatat tctcaaacag ataatcgatg ttgcggtacc
    14641 aaagggcgac acttcgcttc ttttggctta tggggctgcg ttccttgtta tagtgtctgt
    14701 aacaggagcg ttgagctatc tgggcatgat attgcttgct cggctcggac tatcgattgt
    14761 aactcatata aagcaagata tttttttaca ccttttaaag cttccggtgt cttatttcga
    14821 taagcatcct gtcggagagc ttatgtctcg cacggagaca gacaccgaaa gggtgcggga
    14881 tatgttttcg actcttggcg caaacctcat tgtaaatgta cttaccatga tcgggatttt
    14941 cttggtgacc tttgccttga tgcctgcgct cgcacttatc atggtgggca tttcgttg
//
LOCUS       DAOOWY010000045        14599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4160_length_14599_cov_9.302943, whole genome shotgun sequence.
ACCESSION   DAOOWY010000045 DAOOWY010000000
VERSION     DAOOWY010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 14599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14599
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4160_length_14599_cov_9.302943"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK105_04950"
     CDS             complement(<1..183)
                     /locus_tag="PK105_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61090.1"
                     /translation="MGYDASRTTEPLAFRMRPRILDEFVGQEHIVGQGRLLRRAIQKD
                     QLSSVIFAGPPGTGKTT"
     gene            complement(215..1438)
                     /locus_tag="PK105_04955"
     CDS             complement(215..1438)
                     /locus_tag="PK105_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61091.1"
                     /translation="MLNKKRDREFKFIIDRKARENSRKDIPVQSKKRQYLLAAILIIL
                     TLSSCDNTFSIFQSIGQEVKQLGAELFKGTTVRAMGSDATNYYALMSKVVYKPKSGVN
                     WDVLPIDGDTDYFSPGLASDGNTIYVAKADLNANLKDIYSSSDGGKNWTGMDAKSDIQ
                     NVIGAGNNFCVDWLKCENNTLFVEVHDSELAYYLFYYDKLSDSFENAFTSTISSLSEP
                     LVDIVWDGSTSAPGKYWLISSSKVYSGVEGSIDEDSSLNNPALSKGLKGIASDGAGRI
                     LVSRSDGKIYDYASGNWSNFLVKSSSELGPLILLDQPSTSKRILVAMGTSTSGYMECD
                     ENGSIVYENGVGFISTSQSIYNSTVRAKQVEGFWQPVDDSNTLFALLAAGSEGSYALY
                     RNTYSEGAWSGWIAE"
     gene            complement(1449..2045)
                     /locus_tag="PK105_04960"
     CDS             complement(1449..2045)
                     /locus_tag="PK105_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61092.1"
                     /translation="MLKKQMLVTLMISALALGLSAQEKIEDPFEATRVKGDQSISIQI
                     GGFLPLFVIDNAGTPISPSNMSIGSAFSVQYRYMLGKNFSIGGSGAGSFVTTVGGGTL
                     FMAPIGITGAWIWGREPMEYEVSAELGMNIMRLYGNGLISPIAKFGGGISRFVDSSWS
                     IGFKLNWWFVPEIHLGAFSNLTSYANFLEFSIGANYHF"
     gene            complement(2055..2606)
                     /locus_tag="PK105_04965"
     CDS             complement(2055..2606)
                     /locus_tag="PK105_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61093.1"
                     /translation="MLKRKSFDWTPRNSHDVLMALPEGIVCGLFEHRNGQRIDHKNRD
                     FLEHVQRTLVRHSQEICKSFPDIFALSAVEKNDDHTILKLDKAIDSQNLDIALELFAE
                     DADLKVSDNALFYGNKMREGIWFGNGCAVSLDAAISFKKYELMLYLAELPEASFCGFH
                     FRAFARVYCREKMHSHSKEDQII"
     gene            2782..3552
                     /locus_tag="PK105_04970"
     CDS             2782..3552
                     /locus_tag="PK105_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61094.1"
                     /translation="MKRKSEEISCFLIDTISRWPGVDCISMDKRSQQDELDPHYALVL
                     DVYYRRAIPTMEKRQMLFDNPGAFESARGGMKDRFFLDEIPIRVEYKHIPSIQNLVEH
                     PLNHIKLLKNSGTYPLYRLLHFSLVFSRSEWIERMRSNLTNFPEEAWNTLFDSFAAKM
                     EHYLSDFGAASVSGNQFFRLMSHSGFLRYAGASIFMVNHVFEPSHAEFESKLKAQPRL
                     PANFMNLWDSIAGDKNGISEFKKFELARLLAREIFELR"
     gene            3585..5348
                     /locus_tag="PK105_04975"
     CDS             3585..5348
                     /locus_tag="PK105_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61095.1"
                     /translation="MSKIQIAHKPVRWFAHENKGLFRNILTLCVCLCFLFLLLFSSCR
                     SREVHAFGWYDKDGQSLAFTSLLIDKNVGIFERQGEKEKYNLIKAIEIPEGYGIRLLV
                     SSLSNETEFLLNFGEERGKSKSIHCSISEAGSYAFVIPLSEIESIKWLEIKVLKLGSN
                     MSEGNINSLKTASKDDSNAAIDGSSDLSLFSVKELRFVPPFRGMRIQNDITEISPHFL
                     YEEQGEKKHYEIMAPFSGLSPELSSRMKVEIILEGSPGLATFFWGNQKISYLHQGKRS
                     LFVIPYTIFENSPGKIAMDVENDIRASVFCVDPLQSAEEPPSIDPGLLVFHSPLIADP
                     YYLARWDQQPEVLLFLFKDYSVQDRYLKRLAFFVEKMGFVGSLAQDSQIASLHGWNAH
                     DYRAEDLARFFDAAATQNFSLSNEEIELRELLIKNGIILKSGGKYRPGIGAVVSISQE
                     SPSYLQYRLLNHELSHALFFTDTSYRDLVISLYQRLTNDERWFLDRYFRWMRYNVNSS
                     YLMANEMQAYLVQQPIQDLEKYFSRTLANRLVEEHPELTEDINFYMEEHLDALVSCAE
                     QLGSFLESTYGFEPGMLFRAR"
     gene            complement(5469..6074)
                     /locus_tag="PK105_04980"
     CDS             complement(5469..6074)
                     /locus_tag="PK105_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61096.1"
                     /translation="MGKNTKIPPPSKKHEDLLEGLFISPLDDSESLKKMETEEMKILS
                     PLEGRAPLEESHSIPFDDSQGKRVFPLDNTNKREAPEISTRENPSGPESSAQEQLLLR
                     FANELKSIKQDLLSIKEHFKVIREPQIQPSVSPTSVESPSDRLSGASEVQQQALIDEV
                     KRLLLYLDRLLESLPEEKIEEFANSEYFNLYRHVFDELNLS"
     gene            6162..6710
                     /locus_tag="PK105_04985"
     CDS             6162..6710
                     /locus_tag="PK105_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOU61097.1"
                     /translation="MPKIWLMYSPHSSFKTSKRLLGSLSYGLFSFLIAYCFISVVAGK
                     AGILAYKDLALQKQRIQIAIYSLQTQNENKMNTIEELKSNPYAAAEMAAALGYIHQGE
                     VLITLPESWRDAGQIKNNEVNTPILAGESTGLPDPLIRVMSAITGILVFLAIQLFQYK
                     SIEQSAKRISLENRTEPIRTQI"
     gene            complement(6743..9175)
                     /locus_tag="PK105_04990"
     CDS             complement(6743..9175)
                     /locus_tag="PK105_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOU61098.1"
                     /translation="MRFRKPKAIYVFIGLMLVLTATLGAGLGLALAETVNTMRTENFT
                     EFETALPTKIYDINGRLITEFFAEEQRIPISIKELPDHLIEAFITREDQSFYSHNGFN
                     LRSIMRAAIGQILGKSLGGGSTITQQLAGTLYADRRIVSLERKLKELWWALQLERRFT
                     KEEILEMYLNRMIMGPAVYGVEAASRYFFGHPAKECTPAEAAILAIQLSSPSRYNPFR
                     NPMLARERSKEILDQMVDRRVISRAEADESFDAYWASFDYSRIAVSAFYRREDKAPWF
                     SEYVRRQLEDMFYGSIDMYSDGLSVYTTLDLDKQAAADLYMKRGIETANASFKTTMSQ
                     RLTEAERTYVPIIELLGLAFDIPMLYAPQSRSESASQNYFFDIINPILDATSLMLNIP
                     SLKPYVGAASAKIKTELEKSTVEGALVTIDNNTGYILALVGGSDYSQANQLIRATQSK
                     IMPGSTFKPLYYSAAIDSKKITEATYILDEPTTFYNEDGTPYSPQNFKGEWKGSVLAW
                     QALAHSMNVASVKVLQTVGFDAAISRAAALLDITDPIEIRKTFPRYYPLALGVIGVTP
                     LKMARAYAVFANGGKAITPIAIRYIEDRYGNIIAEPEKDALQTLRQKSPQVISSQNAA
                     IMIDMLKRVVFSGTLAGTTQSGSIFKQKTADGHSYVIPIAGKTGTTENWADAWTIGSS
                     PYYTTAVWLGFDRPGNSLGVSQTGATLAAPVWANYMRDIHLGLQYKNFPKPDSGLISA
                     TVCSLSGQLPTEFCSDGTINLLFLEGTEPTHFCELHHPVIAPEPIDSTDRLDFEEPIL
                     SRPEESPIFQNR"
     gene            9375..10460
                     /gene="gap"
                     /locus_tag="PK105_04995"
     CDS             9375..10460
                     /gene="gap"
                     /locus_tag="PK105_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOU61099.1"
                     /translation="MKVAINGFGRIGRLVFQSLVDQNLLGHGEDKIDVVAVVDIVTDA
                     QYFAYQLRYDSVQGKMKADIASKKSDPSQLEDDILVVNGHEISCIMAEKELKNLPWGK
                     LGVDYVIESTGIFTDEKAYGHLEAGAKKVIITAPAKSKAEDKKIPTFLMGVNNDKYDS
                     AKHNVVSNASCTTNCLAPLVYVLLREGIGIETGLMTTIHSYTATQKVVDGVSKKDWRG
                     GRAAAINIIPSTTGAAKAVGEVLPEIKGKLTGMSFRIPTPAVSVVDLTFRSVKETSIK
                     EIDELLKKASSTYLKDILGYTDEELVSTDFIHDSRSSIYDSLATLQNNLPGEKRFFKV
                     ISWYDNEWGYSNRVVDLLRYMASKDNF"
     gene            10482..11741
                     /locus_tag="PK105_05000"
     CDS             10482..11741
                     /locus_tag="PK105_05000"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU61100.1"
                     /translation="MIKTVRDVSLTGKRIIMRVDFNVPMKNGVVQDDTRIVAALPTIR
                     YILDQKPKSLVLMSHLGDPEKDAAKAKEKAEKEGKPFDLEEYLSGKHRMKPVADYLSK
                     LLGVEVVFLPSCFGQKAAIDALPAGSVAMLENTRFHKEETAKDLALQEVLAKELASYG
                     DIYVNDAFGSAHRAHASTATVAKFVPIALGGLLMEKEVANLEPMLHNPPKPMVAIIGG
                     AKVSSKIGVLENLLKNASALIIGGGMAYTFLRAIGVAIGNSLVEEEYIETASELLKKA
                     AAQDVKVVLPVDHIAADHFAPDAKPIIVDTQALPDTLIGLDIGPKTLALYAETLKDAR
                     SVLWNGPVGVFEFDAFAKGTMEVAKLVAEATERGALTVVGGGDSVAAVNKFGLASKIS
                     HVSTGGGASLEYLEGKVLPGIACLEQK"
     gene            11885..12826
                     /gene="cysK"
                     /locus_tag="PK105_05005"
     CDS             11885..12826
                     /gene="cysK"
                     /locus_tag="PK105_05005"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOU61101.1"
                     /translation="MRIYENITDLIGNTPMLYLNKLGKDLPCKIAAKLEFENPGHSIK
                     DRPALSMVLEAEKNGQLHSGMTILEPTSGNMGISLAMIAAVRGYRCLIIMPESMSFER
                     QAVMKAYGAELILTPENQGMSGSIAEAKRLLSMYPDKYFMPMQFENPANTDAHRSGTA
                     QEIFRDTDGKVDIVISGVGTGGTLTGIGESLKEIKPELEVIAVEPAGSPVLSGGSPGS
                     HGIMGIGAGFVPSILNTSIYSEVIAVTDEDAYIMARRAAKEEGLLVGISSGAVIYAAL
                     ALASRPQNRGKLIVGIFASSGERYLSTPLFSDSKAQP"
     gene            12856..13602
                     /gene="cysE"
                     /locus_tag="PK105_05010"
     CDS             12856..13602
                     /gene="cysE"
                     /locus_tag="PK105_05010"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR01172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOU61102.1"
                     /translation="METDIIGNRTSLLRIIRADIETVLQRDPAARTRIEVLLAYPGLH
                     ALWSYRFSHFLWNRGSKLTARILSNTARFLTGIEIHPAARIGTGFFIDHGMGVVIGET
                     AIIGKNVTLYHGVTLGGTSLEKKKRHPTIGDRVTIGAGAKILGDISIGEDSRIGANAV
                     VVKSVPPNSVVVGIPGQVIRRDIPHKAQDKPDLHHERIPDVIGQRISELMQRVSALEE
                     ALTAIEKKGITERRIGNEEDQTDSLGHEVP"
     gene            13712..14461
                     /gene="tpiA"
                     /locus_tag="PK105_05015"
     CDS             13712..14461
                     /gene="tpiA"
                     /locus_tag="PK105_05015"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOU61103.1"
                     /translation="MKRYFIAGNWKMYKTIPEAVELASELKKELADCKEKLMIAPPFT
                     ALHSVAQVIKGSNILLGAQNMGPEESGAHTGEISVLMLKDIGVRVIILGHSERRHTYG
                     ESDALINTKVRLALKHDLEVILCVGETLEEREQGVLENVIRRQLAEGLIGVDASMLAN
                     VTIAYEPVWAIGTGKTATPEDADSVHAFCRRVIAELYGSDIAKNLVIQYGGSVKPENV
                     VALMTKKNVDGALVGGASLKAESFVPIAKFR"
BASE COUNT         4077 a         3047 c         3408 g         4067 t
ORIGIN      
        1 ggttgttttg ccagttccag gtggacccgc aaaaataacg gaactaagct ggtctttttg
       61 gatggcccgg cgtagaagtc ttccttgtcc tacaatgtgt tcttggccta cgaattcatc
      121 gaggatgcgc gggcgcatgc ggaaagcaag gggctctgtt gttctcgaag catcatagcc
      181 tatgtcagaa ctgaaaagag ggtgaggaga gttgctattc tgcgatccag ccactccagg
      241 ctccttcgga ataggtattc cgatagagtg cgtaggaacc ttcgcttcct gcggcaagaa
      301 gagcgaataa cgtgttagaa tcgtctactg gttgccagaa accttcgacc tgctttgcac
      361 gcacggtgct gttgtaaatg ctctgagatg tggagataaa tcccactcca ttttcgtaga
      421 caatagatcc gttttcatca cattccatat agcctgatgt tgaagttccc atagcaacga
      481 gaatacgttt agaggttgaa ggttgatcca aaaggataag aggcccaagt tctgaagagc
      541 tctttactaa aaagttggac cagtttcccg atgcatagtc atagattttt ccatcgctcc
      601 tcgaaacaag gatacgacca gcaccatcgg atgcaatacc cttaaggcct tttgaaaggg
      661 ctggattatt cagacttgag tcctcgtcaa tggagccttc tactccactg tatactttac
      721 tggatgatat aagccaatac ttgcctggcg cggaggtgga tccatcccat acaatatcta
      781 cgagaggctc ggaaagactg gaaatagtgc ttgtaaaggc attctcaaat gagtccgaaa
      841 gcttgtcata atagaacaga taataagcta gttctgagtc gtggacctcg acaaaaagcg
      901 tattgttctc gcatttgagc caatcaacgc aaaaattatt gccagcacca attacatttt
      961 ggatgtcgct ctttgcgtcc ataccagtcc agttcttacc tccatcgcta ctcgaatata
     1021 tatctttcaa attagcgttt aaatctgctt tggcaacata tatagtattg ccatcggatg
     1081 caagacctgg tgaaaagtaa tctgtatctc catctatagg aaggacatcc caatttacgc
     1141 cgctttttgg cttgtatacc accttagaca tgagggcata ataatttgta gcatccgagc
     1201 ccattgcgcg cacggttgtt cccttaaaaa gctcagcgcc caattgcttc acttcttggc
     1261 ctatgctctg aaaaatcgaa aaagtattgt cacagcttga caaggtcaga atgatcagaa
     1321 ttgcagctaa aagatattgc ctctttttgg actggactgg gatatccttt ctggaattct
     1381 ctcgagcttt tctatcaata ataaacttga attctctgtc tctcttctta ttaagcatgt
     1441 ggcacccctc agaaatggta gttagcacca atggaaaact ctagaaaatt ggcatagcta
     1501 gttaaattgc taaaggctcc aagatgtatc tctggaacga accaccaatt taacttgaat
     1561 ccaatactcc atgaggaatc tacgaatctg gagattcctc ctccgaactt ggcaatagga
     1621 gaaataagac cattaccata gagtcgcata atattcatac ctagttcggc agaaacctca
     1681 tattccattg gttcgcgacc ccatatccat gctccggtta ttccaatcgg agccataaaa
     1741 agcgtgcctc cgcccacggt agtcacaaag gagcctgcac ccgaaccgcc tatggagaaa
     1801 ttctttccca gcatatagcg atattgaact gaaaatgccg atccaatact catgtttgat
     1861 ggtgatatag gcgttccagc attgtcgatc acaaaaaggg gcaaaaagcc tccgatttgt
     1921 atgcttattg attggtcccc tttgactcgt gtcgcctcaa aggggtcttc gattttttct
     1981 tgcgcactaa gaccaagcgc caaggcggaa atcattagag taaccaacat ttgttttttc
     2041 agcatcagcc ctccctaaat tatctgatcc tctttcgaat gagaatgcat cttctctcta
     2101 cagtagacac gagcaaaagc tctgaagtga aaaccacaga acgatgcttc tggtaactcc
     2161 gctagatata gcataagttc atatttcttg aaactgatgg ctgcatcgag agaaactgca
     2221 catccattgc caaaccagat cccttcacgc atcttattac catagaaaag agcattgtcg
     2281 gatactttca gatcagcatc ttcagcaaaa agctcaagag caatgtcaag attttgcgaa
     2341 tcgattgctt tgtcaagctt caatatcgta tgatcatcat tcttttccac tgccgatagc
     2401 gcaaagatgt cagggaagct cttgcagatt tcttgagagt ggcgcacaag ggttcgctga
     2461 acatgctcca agaaatcgcg attcttatga tctattcttt ggccatttcg gtgttcgaat
     2521 aagccacata cgatgccttc aggcaatgcc atcagaacat cgtggctatt tcttggagtc
     2581 caatcaaagc tctttctctt gagcatccat atctgttttc taatatttcc ataaggcctt
     2641 aaaattagta caaagatctg catgtcaatt gagtatatag tcacttaggc tcttggttaa
     2701 catgggtgaa agagtaccat cgaagatgca tggcagaaag ccagcgtcca tgatataatt
     2761 ggccttggaa gggtagtgta catgaagcgc aaatctgaag agatatcgtg ctttcttatc
     2821 gatacgattt ctcgctggcc aggtgtggat tgcatatcta tggataagcg ctcccaacaa
     2881 gatgaattag acccacacta tgcattggtt ctcgatgtat actatcggcg cgccattcct
     2941 accatggaaa aaagacaaat gctcttcgac aatccaggtg cgttcgagtc ggctcgtggt
     3001 ggtatgaagg ataggttttt tctagatgaa atacccataa gggtagagta taagcatata
     3061 ccaagcattc agaatctggt agaacatccg cttaatcata taaagctcct aaagaattca
     3121 ggtacttatc ctctctatcg cttacttcat ttttcgcttg ttttttcgag gagcgaatgg
     3181 atcgaacgca tgcggtctaa tctcaccaat ttcccggaag aagcttggaa tacgctattc
     3241 gatagctttg ctgcgaagat ggaacactat ctctccgact ttggggcagc atcggtatca
     3301 ggtaatcaat tttttcgctt aatgtcgcac tcaggatttc taagatatgc aggtgcttcg
     3361 atttttatgg tgaatcatgt ttttgaacca tctcacgccg aattcgaatc aaagttaaag
     3421 gctcagccaa gactcccggc gaattttatg aacctatggg attcaattgc cggcgataag
     3481 aatggtattt cggagttcaa gaaattcgag ctcgctcgat tgcttgcacg agaaatcttc
     3541 gagctccgat agacaaaagc cttagataga gatcttagat agagatgagt aaaatacaaa
     3601 tagcgcataa gccagtccga tggtttgcgc atgagaataa gggtctattt cgcaacatac
     3661 tcactctctg tgtatgtttg tgtttcttat ttctccttct ttttagctcc tgcagatcta
     3721 gagaagtcca tgcttttgga tggtatgata aggatgggca aagtctagca tttacatccc
     3781 tcctgatcga taagaatgtt ggtatcttcg agagacaggg cgagaaagaa aaatacaatc
     3841 tgattaaagc aatagaaatt cctgaaggat atggtattcg tctactggta tcatctttga
     3901 gcaatgagac agaattcttg ctcaattttg gcgaggaaag aggtaaatca aagtcaatac
     3961 actgctcgat ttcggaggct ggaagctatg cttttgtcat tcccctttca gagatcgaat
     4021 ccatcaaatg gctcgagata aaagtgctaa aacttggttc caacatgagt gaaggcaata
     4081 ttaatagcct taagacagct tccaaagatg attcgaacgc agcaattgat gggagctccg
     4141 atttgtctct tttctctgtt aaggagctac gttttgtacc tccgttccgc ggtatgcgga
     4201 tccagaatga cattacagaa atatcgcccc attttttata cgaagaacaa ggcgaaaaaa
     4261 aacactacga aatcatggct cctttttctg ggctttctcc tgaactttct tcaagaatga
     4321 aagtggaaat catattggaa ggaagtccag gtctggctac ctttttttgg ggaaatcaaa
     4381 agataagcta tcttcatcaa ggaaagagaa gcctctttgt aattccttat acgatttttg
     4441 agaacagccc cggtaaaata gccatggatg ttgagaatga catcagagcc tcggtttttt
     4501 gtgttgatcc attgcagtcg gccgaggaac cacccagtat tgacccgggt ttgctcgttt
     4561 ttcattctcc gctgattgca gatccatatt accttgcgcg atgggatcaa cagcccgaag
     4621 ttcttttgtt cttattcaag gattattctg ttcaagaccg ttatctaaag aggcttgctt
     4681 tttttgttga aaagatggga tttgttggaa gcctggcgca agacagccag atcgcgagcc
     4741 tccatggctg gaatgctcat gattatcgag cagaggacct ggcgaggttt tttgatgcag
     4801 ccgctaccca gaatttctcg ctatcgaatg aggaaattga attgagagag ctattgataa
     4861 aaaatggaat tatcttaaaa agcggaggca agtatcgacc aggcataggg gcagtagtat
     4921 cgatttcgca agaatctccc agctacctgc aatatcgcct gctcaatcat gagttgagcc
     4981 atgccctttt ttttacagat accagctacc gcgaccttgt catttctctc tatcagagat
     5041 tgactaatga tgaaagatgg tttctcgatc gttatttccg ctggatgcga tataacgtta
     5101 attcatcata tttgatggct aacgaaatgc aggcttattt agttcagcag ccaattcaag
     5161 atttagagaa atatttttca agaacattag cgaatagact tgtagaggag catcctgagc
     5221 taacagaaga tatcaatttt tatatggaag agcatcttga tgcacttgtg tcatgtgctg
     5281 aacaactggg ttcttttcta gagtctactt atggatttga gccaggcatg ctttttagag
     5341 caaggtagag caaggtagcc gcactgggat tttttgcgct attgaatatc ggccttcatt
     5401 tctggttttt cctttatgca tgctatgatt ctgttgtgga tttctcgaat tatagtttcc
     5461 aagattgctt aagaaaggtt cagctcatca aatacgtgtc tatatagatt aaaatattca
     5521 gaatttgcaa attcttcgat tttttcttct ggcaatgatt ccaggagacg atcgaggtag
     5581 agcagcagtc tcttcacttc atcaatcagg gcttgctgtt ggacttccga agctccagat
     5641 aatctatcgg aaggagattc cacggatgtg ggagataccg aaggttggat ctgcggttcc
     5701 cttataacct taaaatgttc ttttatagat agaagatctt gcttgattga tttcaattca
     5761 ttggcaaatc tcagtaaaag ctgttcctga gccgagctct ctggtccgga tggattttct
     5821 cttgtgctga tttcaggcgc ttcccttttg ttcgtattgt ccaaagggaa gacccgcttt
     5881 ccttgagagt catcgaaagg aattgagtga gattcttcta aaggagctct tccttctaac
     5941 ggagagagaa ttttcatttc ttcggtttcc atttttttga gagactccga atcatcaaga
     6001 gggcttataa aaaggccttc gagcaagtct tcatgctttt tgctcggtgg tggtatcttg
     6061 gtatttttcc ccatcgcggt cttcctcttt aagaatacta caccctttaa ctcatacagg
     6121 gaactacttc ctgcagaaat gcctcatcag aattgatatt tatgccgaaa atttggttga
     6181 tgtattcgcc tcattcttca ttcaaaacaa gcaagaggct gcttggctct ctttcttatg
     6241 gcttattctc atttctgatt gcgtactgct ttatctctgt cgttgcaggc aaagcaggaa
     6301 tactagccta taaagatcta gccttgcaaa aacaacggat tcaaatagca atctattctc
     6361 ttcagactca aaatgagaac aagatgaata caatcgagga acttaaaagc aatccctatg
     6421 ctgccgcaga gatggctgcg gctttaggat atatccatca aggagaagta ctgatcacac
     6481 ttccggagtc atggagagat gccggtcaga taaagaacaa tgaagtaaac actcccattt
     6541 tggcaggaga gtctacagga ctaccggatc cactgattcg cgttatgagt gcaatcacag
     6601 gaatactcgt ttttttggcc atacagcttt tccagtacaa atccatagaa cagtcagcaa
     6661 aaagaataag cctagagaat cgaaccgagc cgatccgcac ccagatttaa taagtttgtc
     6721 aacttaagtc ctactcatat tcttatcgat tttgaaaaat aggtgattct tcaggtcttg
     6781 aaagaatagg ttcttcaaaa tctagccgat ctgtcgagtc aataggctca ggagcaatga
     6841 cagggtgatg cagttcacag aaatgagtgg gctctgtacc ttccagaaaa aggagattta
     6901 ttgttccatc agaacagaat tcagttggga gctgtccgga gagggagcaa accgttgcag
     6961 aaatcaggcc tgaatcaggc ttggggaagt tcttatattg caggccaaga tgtatatcgc
     7021 gcatataatt agcccaaact ggagctgcaa gtgtggctcc tgtttgagaa accccaagag
     7081 agttgcctgg acgatcgaag ccaagccata cggcggttgt gtaatatggg cttgaaccga
     7141 tagtccatgc atcagcccaa ttctccgttg tgccagtctt acccgcaatg ggaattacat
     7201 atgaatgtcc atccgctgtt ttttgcttga agatagagcc tgattgtgtt gtgccggcaa
     7261 gagttcccga aaatacaact ctctttagca tatcgatcat tatcgctgca ttctgcgatg
     7321 atattacttg cggggatttt tgccggaggg tctgtagtgc atctttttca ggttcagcaa
     7381 tgatattgcc ataacgatct tcgatatatc ttatggcaat gggtgttatt gcttttcctc
     7441 catttgcaaa tactgcatag gctcttgcca ttttgagggg tgtgacaccg attactccta
     7501 gcgctagtgg ataatatcga gggaaggttt tacgtatctc tattggatcc gttatgtcga
     7561 gaagagccgc tgctcgcgat atagctgcat cgaagccaac tgtttgaagc accttgaccg
     7621 atgcgacatt catagaatga gcaagggctt gccatgcgag aacagacccc ttccactcgc
     7681 ccttgaaatt ttgaggagag tagggggtac catcttcatt gtagaaagtt gttggttcat
     7741 cgagaatgta agtcgcttcg gtgatttttt tggaatctat ggctgcagaa tagtataggg
     7801 gcttgaaggt tgatccaggc ataattttag actgggtcgc acgaatgagc tgatttgcct
     7861 ggctataatc tgagcctcca acaagcgcaa ggatatagcc tgtattgttg tcgatcgtca
     7921 caagtgcacc ttccacggtg ctcttttcga gttcggtctt tatcttggcg gaagctgctc
     7981 caacataagg ctttagagaa ggaatgttga gcattaagct tgttgcatcc aaaattggat
     8041 taatgatgtc aaagaagtaa ttctgtgaag ctgactcgct tcgactctgt ggagcataga
     8101 gcatcggaat atcaaaggca agacccaata gttcgataat gggtacataa gttctctctg
     8161 cttctgtgag gcgttgcgac atggttgtct taaaggatgc attggcagtc tcgatacctc
     8221 gcttcatata gagatcagct gccgcctgtt tatctagatc tagcgtggta tagaccgata
     8281 atccgtcgct atacatatct atggaaccat aaaacatgtc ttccagttga cgtcttacat
     8341 attcgctgaa ccatggagcc ttatcttcgc ggcgatagaa tgcagataca gctattcttg
     8401 aataatcaaa agaagcccaa taagcatcga aggattcatc ggcctcagct ctagaaatga
     8461 cacggcgatc caccatctga tcgagtattt ccttcgaacg ctcgcgagcg agcatgggat
     8521 ttcggaaagg attgtaacgc gatggacttg agagttgaat tgcaagaatg gccgcttccg
     8581 caggagtaca ctcctttgct ggatgaccaa aaaagtagcg gctggctgcc tcaacaccat
     8641 agacggctgg tcccattatc atgcggttga gatacatttc tagtatttct tccttcgtga
     8701 aacggcgttc cagctgtaga gcccaccaga gttcctttaa ctttcgctcc aggcttacta
     8761 ttctacgatc agcatagaga gtaccggcta gctgctgagt tattgtagat ccacctccaa
     8821 ggcttttgcc cagaatctga ccaatagccg cgcgcataat ggatctgaga ttgaatccat
     8881 tatgcgaata gaatgactga tcttctcgtg taataaaagc ctcgataaga tgatctggta
     8941 attcctttat cgaaatagga atcctttgct cctcagcaaa gaactctgta attagtctgc
     9001 cattgatgtc atagatcttt gtgggaagcg cagtttcgaa ctcagtgaag ttctctgtcc
     9061 tcattgtatt gacggtttcc gccaaggcaa ggccaagtcc agctcccagc gttgccgtca
     9121 gaaccaacat tagcccaatg aagacatata ttgccttggg ctttcgaaat ctcatggctt
     9181 tagattatag atatgatgat gttttggtca atcttacata atgaagaagg tatagaagtt
     9241 ttcaggaaat ttgagtagat tgatgcgaat cgattgactc ttttgcctaa tcgatactat
     9301 tttattttta ttctgagccg atcacaggtg ccttttgagc cccgtgtcaa acccagttcc
     9361 cgaggaggaa catgatgaaa gtagcaataa atggttttgg aagaattgga aggctggttt
     9421 tccagtctct tgtagatcag aatcttcttg gccatggaga agacaaaata gatgtggtgg
     9481 cggtggtcga tatcgttact gatgcccaat attttgctta tcagctgaga tatgattctg
     9541 tccaaggaaa gatgaaagca gatatagcct cgaaaaagag cgatccatcg caattagaag
     9601 atgacatcct tgtagtaaac ggccacgaga tttcctgtat catggccgaa aaggaactaa
     9661 agaaccttcc atgggggaaa ctaggggttg actatgtgat tgaatccacc ggtatcttta
     9721 cagatgaaaa agcctatggc caccttgaag ccggagccaa aaaagttatc ataactgcgc
     9781 cggcaaagag caaggctgag gataagaaga ttccaacatt cctcatgggt gtcaacaatg
     9841 acaaatatga ttctgcaaaa cacaatgttg tttcgaacgc ttcgtgcacc acaaattgcc
     9901 ttgctcctct cgtttatgtg cttctcagag aaggtatagg cattgaaact ggcctcatga
     9961 ctaccataca ctcctatact gcaacgcaga aggtggtcga tggagtgtcc aagaaggatt
    10021 ggcgcggcgg tagagcagcc gctatcaata tcattccttc tactaccggc gctgcaaagg
    10081 cagtggggga agtgcttcct gaaataaaag gaaagcttac aggcatgtcc ttccgcattc
    10141 ctacgccggc agtctcggtt gtcgatttga catttcgctc ggtaaaggag acctccataa
    10201 aagagatcga tgagctattg aagaaagcct ccagtaccta cttgaaggat attcttggat
    10261 ataccgatga agagcttgtc tccactgatt ttatccatga tagtcgttct tcgatctacg
    10321 acagcctggc tacgcttcag aacaacttgc ccggagagaa gcggttcttc aaggtgattt
    10381 cttggtatga taacgaatgg ggatattcga atagggtagt tgaccttctt cgctatatgg
    10441 cgtcgaagga caatttctag gatccgtcta aggagatcgc aatgatcaag acagtccggg
    10501 atgtttcctt aacaggcaag aggataataa tgcgtgttga tttcaatgtg cccatgaaga
    10561 atggggttgt acaggatgat acgcgtattg tagccgcgct gccaactata aggtatatcc
    10621 ttgatcaaaa gccaaaaagc cttgttctga tgtcacatct tggagatccg gaaaaagatg
    10681 cggcaaaggc taaagaaaaa gccgaaaagg aagggaaacc tttcgatctg gaagaatacc
    10741 tatccggcaa acaccgcatg aaacctgtag cggattatct ttcaaaactt cttggtgtcg
    10801 aggtggtctt tttgccatct tgttttggtc aaaaagccgc gattgatgct cttcctgcgg
    10861 gatcagtggc gatgctcgag aatacgcgct tccataaaga agaaacggca aaggacctgg
    10921 cattgcagga agtcttggca aaagagcttg cttcctatgg tgacatttat gtaaatgatg
    10981 cttttggatc tgcccataga gcccatgcca gcactgcgac agttgctaag tttgtgccga
    11041 tagcgcttgg tggtttatta atggagaagg aggtggcaaa tcttgagccc atgcttcata
    11101 atcctcctaa accgatggtc gcaattatcg gaggagcaaa agtatcctca aagataggag
    11161 tgctggaaaa tctactaaag aatgcttctg ctttgataat tggaggaggt atggcttata
    11221 cctttctcag agcaatagga gtggctattg gcaacagtct ggtggaagaa gaatatatag
    11281 aaacagcaag tgagcttctc aagaaagctg ccgcgcaaga tgtaaaagtt gttcttccag
    11341 tagatcatat tgctgctgac cattttgcgc ccgatgcaaa gcccataatc gtagatacgc
    11401 aagctttgcc tgatactctc atcgggctcg acatcggtcc aaagacgctg gctctgtatg
    11461 cggaaactct caaggatgca aggagtgtcc tatggaatgg accagtgggg gttttcgaat
    11521 tcgatgcttt tgcaaagggc accatggagg ttgccaaact tgttgcagaa gcaacggaga
    11581 gaggggctct tacggttgtg ggaggaggcg attcggttgc cgctgtcaac aaattcggac
    11641 ttgcctccaa gattagccat gtttcgacag gtggaggagc atctctggaa taccttgaag
    11701 gcaaagttct tcctggtatt gcgtgtctcg aacaaaaata ggcctccctt tcggttgagt
    11761 aagcatatag cggccgcccg aaagggtggc tgttttttta tctaatgtga tcgattcgga
    11821 ttccgatccg cgccgaccat tattcatgat tgaacgatag attttataga ggaggagtcc
    11881 aaaaatgcgt atttatgaga atatcacaga tctaattggc aatactccga tgctttatct
    11941 caataagctt ggcaaggatt tgccatgcaa gattgcagca aagcttgaat tcgagaatcc
    12001 aggccattct ataaaggata ggcctgcgct atccatggtg ctggaagctg agaaaaacgg
    12061 gcaattacat tccggaatga ccattttaga gccaactagc ggcaatatgg ggatttcgct
    12121 cgctatgatt gcggcagtgc gcggctatag atgccttata atcatgccgg agtcaatgtc
    12181 attcgaacgc caggccgtta tgaaagccta tggagcggag ctgattctaa cgccggaaaa
    12241 ccagggaatg tcgggatcca ttgctgaggc taagcgatta ttatcaatgt atccagataa
    12301 atatttcatg cctatgcagt tcgagaatcc cgctaatacc gatgctcata ggtcgggcac
    12361 agcgcaggag atttttcgag atacggatgg gaaggtcgat attgtaattt cgggagttgg
    12421 aacaggaggg actctaactg gaataggtga atcattgaag gagataaaac cagaattgga
    12481 ggttatagcc gtagagcctg cgggatctcc ggttctctcc ggcggatctc ctgggtctca
    12541 tggaataatg ggaattggtg cgggcttcgt accatcgatc ctgaatactt ctatctatag
    12601 tgaagtaata gcggtcaccg atgaagatgc ttatatcatg gctagacgcg cagcaaagga
    12661 agaaggactg ctagtaggaa tctcttcagg agctgtcata tatgctgcgc tcgcgcttgc
    12721 ctctcgaccc cagaacagag gaaagctaat tgtgggaatc tttgcttcaa gcggagaacg
    12781 atatctttct acaccgctct tttcggattc gaaggctcag ccataaagtg aatagagata
    12841 ataaaaggaa ttcagatgga aacagatatt ataggcaata ggacatctct tttgagaata
    12901 atcagggcgg atatcgaaac cgttctccag cgcgatccag cagcaaggac aagaattgag
    12961 gtattgcttg cttatccagg tttgcatgcg ctctggagct ataggttttc tcatttttta
    13021 tggaatcgtg gatcaaagct tactgctcgt attctgtcca ataccgcaag atttctcacg
    13081 ggcattgaga ttcaccctgc cgcaagaatt ggaacaggct ttttcatcga tcatggcatg
    13141 ggagtggtaa taggagaaac cgccataata ggcaagaatg taacgcttta tcatggtgtc
    13201 actctcggag gtacttctct cgagaagaaa aagcgtcatc ctactattgg agatagagtt
    13261 actattggag caggagcaaa gatcctaggc gatataagca taggcgagga ttcgcgtatt
    13321 ggggctaatg ccgtggttgt aaagagcgtt ccacccaatt cagtggttgt tggaatacct
    13381 ggtcaagtaa ttaggcgcga tatcccacat aaggcccaag ataagccaga tcttcaccat
    13441 gagagaattc cagatgtaat tggacagcga attagcgaac ttatgcaaag agtcagtgct
    13501 cttgaagaag cgctgacagc cattgagaaa aagggaataa cagaaagaag aattggaaat
    13561 gaggaggacc aaaccgactc tcttgggcat gaagtgccat agctttacca aaaaaatcga
    13621 taagtatagt atgcatgaaa taagcttgct tctctctggt taactgaggg cagctactat
    13681 gtaagcatca tctcgaggga gaaataaaac gatgaaacgc tattttattg caggcaattg
    13741 gaaaatgtac aaaaccatac ctgaagccgt cgaattagca tccgaactaa agaaagagtt
    13801 agctgattgc aaagagaaac tcatgattgc gccgcctttt acggcgctgc attcggttgc
    13861 tcaggttata aagggcagca atattttgct tggggcacag aatatgggcc ccgaggagag
    13921 cggggcgcat actggcgaga tttcggtgct tatgctaaag gatatcggag ttcgggtaat
    13981 aatactgggg cactcagagc gcagacatac ttatggtgag agcgatgcgc tcataaacac
    14041 aaaggtaaga cttgcactaa agcatgatct cgaagtaatt ctctgtgttg gcgagacact
    14101 cgaggagcgc gagcaaggcg ttctagagaa tgtaatccga cggcagctgg ctgaaggact
    14161 cataggggtc gatgcttcca tgcttgccaa tgtgaccatt gcttatgagc ctgtctgggc
    14221 tatcggcaca gggaaaaccg ctacaccgga agatgccgat tctgttcatg cattttgtcg
    14281 ccgagttatc gctgaacttt atggctccga tatcgcgaaa aacctagtca tacaatatgg
    14341 cggctcagta aagccagaga atgttgttgc tcttatgaca aagaagaatg tcgatggcgc
    14401 attagtggga ggcgcaagcc tgaaggctga gagctttgtg cccatagcta agttccgttg
    14461 attattagtc caatagcgag gtattgcttc attggggact ttgcgctagt atggcttttc
    14521 ggattgcgaa tttacctgaa aagctttttt ctgcagtcaa atgaacttgc aactggagag
    14581 agagaatggg tttcttcag
//
LOCUS       DAOOWY010000046        14337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4252_length_14337_cov_11.528567, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000046 DAOOWY010000000
VERSION     DAOOWY010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14337
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4252_length_14337_cov_11.528567"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PK105_05020"
     CDS             complement(<1..684)
                     /locus_tag="PK105_05020"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOU61104.1"
                     /translation="MTQELWLFILMVGAFASGCFLFKWPVSVAMLVASALAAIVGGVQ
                     DPIRHLVEGTFGYLDTILTIATAMMFMTGFRDSGALQALTAAVIKKFNKQPALLMILL
                     TLVIMVPGMITGSSTASVLTAGAVVAPMFIAMGMSTIDAGALISIAAILGMIAPPVNI
                     PVMIIGGGVDMPYVGFEIPLILLTFPLAIIFALWFGWRNVRKVDRSNVLATLDFSELN
                     KYGGLRLFSP"
     gene            complement(695..1276)
                     /locus_tag="PK105_05025"
     CDS             complement(695..1276)
                     /locus_tag="PK105_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOU61105.1"
                     /translation="MRNARRIIPILLVPLLFFGGGAILPLAAYNLKAAFAPQPALLTS
                     IGQSADVEMAKTIMARAKLNFTMDPTIKAQALASTNAKTLVIVIGGSSKGLGAAGISA
                     EAELERTKALLAEAKKRGMKIIGLHIGGEARRGELSDRFINEAVPSCDYFIVVEEGNK
                     DGLFTKLCGNKIPLDIVQKISQIGEPLAAAFLK"
     gene            complement(1477..3162)
                     /gene="ggt"
                     /locus_tag="PK105_05030"
     CDS             complement(1477..3162)
                     /gene="ggt"
                     /locus_tag="PK105_05030"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOU61106.1"
                     /translation="MKKSILALMLLIAAIATISAQLPVNLYGRAAVGENGVVAAAKPE
                     ASQVGIDILKKGGNAVDAAIATGFALGVLEANANGIGGGGFMIIKMVDMAEPVVIDFR
                     EKAPGKATPTMYLGPDGKVIPNSTIEGGLSVGVPGEVKGLLYALENYGSGKLSRADII
                     QPAIQWALQGIPVTVNLESIIKDNYGKLVKYENGAQIYLKDGLPYEVGDVIYNPDLAR
                     TLAKIVKEGADAIYKGPIAQAMVDEVQKRGGILTLEDLANYEIKVRKAVEGEYRGYKV
                     YSVPPASSGGTHLIEILNILENFDVVKLGFQTAQASHLWSEILKLTFADRAKYMADTD
                     FVQVPLKGLTNKEYAKQQATRINMNASMSSPQPGDPFKYESGSTTHFSVMDKAGNMVA
                     VTKTINYFFGSGVVIPGWGFIMNDEMDDFVPTPGSVNSVEPGKRPLSSMSPTLVLDPE
                     GRSFMAIGSPGATRIFPTVAQVISNVIDFGFPIQEAILAPRLFQAASGSLNLEGRYPV
                     SALDGIKKLGHDVTIRGDWDSYFGGVHAVLYDYNEGLLYGGADPRRDGQAAAF"
     gene            complement(3401..4705)
                     /locus_tag="PK105_05035"
     CDS             complement(3401..4705)
                     /locus_tag="PK105_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HOU61107.1"
                     /translation="MCDTLILHNSRGNPFIFGKNSDRHPEEPQALVYIPAQEPKVAKL
                     GEGIEYPDMSYDFLLSKPSWIDGGEMGLNNRGLAIGNEAVFSRFKPDKKGVLGMDILR
                     AALSVCATAKEALNFISSFVERFPQGGNGAYKGKLFYNNSFLIADPDEAYILETAGPR
                     WAWRKAMIADAISNIYCIEDDFTCIDAMTRKEIALVNDKGDCSDETDRDRKEQRGSWK
                     KYVEDKKYLFFTQGEQRRSTSLAGLLRIAAESDKTEGGQILFSSAIEPFFAILRSHKG
                     ASKPGAFLNRMKNVCIHPGIFPKSATTASMVIEYCKGGAIIWYTDSSFPCVSLYKPVL
                     LKDGHFYALWKPLLTENNAEKSYSYWEARKNWASKPRKLELSSQQAFVQSRDIAQKSI
                     VEIAHQAFPAMIKEKTSTERMLSVYASEVAAIVGEWEEHWIN"
     gene            4901..5746
                     /locus_tag="PK105_05040"
     CDS             4901..5746
                     /locus_tag="PK105_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61108.1"
                     /translation="MNSLLHAQTNSVVLKESCQSSPPKNPAQIGKFQFDSQGFLSSNQ
                     DLLPGIPFPKGMMPPEVEAFLELRDNVYENAAPESVEAQAVSLLKRLSSLPVSISNQA
                     LLEARIAYLVGRAWNDHKDKNKAIPWFERAVEAAQRMVNLEGETPTALVVLAEPLGEL
                     SILKDLGFLIKNGPKVGQYASKALELDPKNIKAWLLKASALAYPPPIWGGNYKKALET
                     YASVLLIAQNGLPKDVLFDLRVGIATAYSNLKLSEHATWWFDAALLLYPKNLYAKSEL
                     EKLSL"
     gene            5743..6882
                     /locus_tag="PK105_05045"
     CDS             5743..6882
                     /locus_tag="PK105_05045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU61109.1"
                     /translation="MISTLLNAYGLIVFAACVFFFFITVSNILWLMHTLTIERKKNGP
                     SVAVLIPARNEALRIRPCIDSLLSQDYKSYQIYVIDDNSDDETWNILKSYMIRFPSKF
                     KAFKAEPLPENWYGKPHALQELATHVKENYLLCTDADTIHKPDSISKAMAAAEKYKAD
                     LITGYAHHLMPTFAEAAIVPSMYILTMLGMPLYLIPNTKSPLFSHAIGQFMLFKRSFF
                     EKIGGYEKVKHEATEDVRLSRIIKENGGKIAFIDLKDIVECRMYTTYKEAIQGIAKNA
                     YDYLGKNLLILFSGTVAVPLVFYVPLIVLFIDVPWLGQAIPYLKASAIITFYSWVLET
                     IDRRLPWYVPFIYPLIFVNTMSALWRGFHLVNKEGGIEWRGRKVK"
     gene            6879..7664
                     /locus_tag="PK105_05050"
     CDS             6879..7664
                     /locus_tag="PK105_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU61110.1"
                     /translation="MRYRKGASLVSDTAISRLIGDVLLAFIGLIAWPLCHLLYNIKIE
                     KIGRAKTERLPSRAVLVSNHCMPLDPVFHGLSIFPRRTFFTLLEETCEAPVIGPLVRL
                     LGGIPLPRNRNRLGDIEMAVEHALNTRGLVHFYPEGECFLGNQDIFPFKAGAAYFAIK
                     FGVPVIPIVTILKRANGRKSKRIQVNVHMLEPIMPLALLDKPLSLSSMAKNPAQENSR
                     EMLSRAIECTRKFHALMQAEIDFSGCDKSLYKGPMPRIKGVND"
     gene            7856..9586
                     /locus_tag="PK105_05055"
     CDS             7856..9586
                     /locus_tag="PK105_05055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU61111.1"
                     /translation="MERFLERSGYAEDRILQFFARLIGDQFFVQKKDILVHDEDLGYR
                     SPLITALIESYIEDSKTSATSKISGSEEETREKQARIRALVLTEHEEVLIDLQKLFAI
                     ASFDSLVISSDTALKEANMPLSERTVVAMPTSLFLAATQVPEFKAREFGLVVLEGADL
                     FGDTPGEVQRRIWGYLLPPWQRRAAIFAKHIGIRTRNTALDFANDPKTIVLKKAQASL
                     SQISTYMYRLSSQHKFRVLLAMIQENFQEDRRAIIVFCNLRQTSMELEFRLKLNNIKA
                     KQVSISAPKAERKELLLEFAEIEKKAEGGQFVLVVSNDNLSVLPSELANLAIHYDIPL
                     DPDIYLERVRIMRPSSSTIIGLVCERYEVGLSAINSKFGMQFELREPSYDMLEYRDES
                     EGVPLKIEYERRSSEYKPRRRIEQSKDKTEDAKRIKKTQHRELKENFHQGQKAQHNRK
                     NSSPKKSPGVDQNSELYSMSTDERLAYFRQKYRGILNSPIEPKPHPKKGEKTISRSAS
                     EPLSFENTIPTKNADGERSISAADSIEVRKGDKKQASGMESDNPSIVKRFIEKLFPGT
                     NEGTDEGIKE"
     gene            9769..12309
                     /gene="glgP"
                     /locus_tag="PK105_05060"
     CDS             9769..12309
                     /gene="glgP"
                     /locus_tag="PK105_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOU61112.1"
                     /translation="MKILSHTVKPALKGRLTKLDLIARNLWLSWNFDAVSLFTRIDRG
                     LWSASGQNPVRMLGMLSQEKIDELASDPAFLSDLDEVYSNFQRYLKNRIWYRGPRDRV
                     IAYFSMEYGLDVSLATYSGGLGVLSGDHLKTVSDLGLPLVAVGLLYRQGYFKQYLNAD
                     GYQLETYPENDWYNMPVERCVDAQDAPVLIQVEMAGRPISAGIWRVDIGRAQLYLLDT
                     NIPENKPEDRIITATLYGGDMEMRIKQEILLGIGGIRALKALGIHVGATHMNEGHSAF
                     LALERIRVLMEEYGLDAHSAIQAFIPTNVFTTHTPVPAGNERFGIELMEKYFRSFVTA
                     LGLDWNEFLGMGRENPLDKQESFCMTVFALRLSVRSNGVSKLHGEVSRRMWKGLWPEL
                     DLKEIPIGHVTNGVFPRTWISHDMLSLLDKFLGPKFELEPDNPDVWAAIDQVSDEELW
                     RTHERRRERLVAISRQRLAATYKRLSLPDGEIVRSSDVLSPYALTLSFARRFATYKRG
                     TLLLKDPDRLIRLLNDKDKPIQIVIAGKAHPHDMAGKELIREIIHFSRREEVFGRLVF
                     IEDYDMAMGRYMTSGSDVWLNTPRRPQEASGTSGMKAGMNGVLNCSVLDGWWAEAYSS
                     EIGWAIGSGEEYKDEALQDEIEAEDLYDLLEHEILPLFYTRGRDNIPRGWLTKMRSSM
                     KTIGSKFATHRMLKEYYTNYYEPALRESKTLEADNYRASVSLAQYIEKVKRVWNGVRI
                     VEFSDDGAPVVPRGSAIEVRALVDLAGLLPDDAAVECYLGRLSSKGEILEGTRIPMTL
                     LGPEGQYYRYQCDIRSEITGQIGYSVRVLPAHPALDGRFVPGLVRWAQ"
     gene            12473..13078
                     /locus_tag="PK105_05065"
     CDS             12473..13078
                     /locus_tag="PK105_05065"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOU61113.1"
                     /translation="MRKGFFILLLVALLSCIGPFSVFAAISGDPIKQYGLPFLITNAG
                     QGPGGKMARLLVSQTKTLELDKDFFYLAEPVEADLKARPYKALMVVIGSSDKGLGASG
                     ITIDQEIARLQRIVKASKDMKLPIIAVLLEKDKRSNVATNPNERCIDTICPYAEWMIV
                     VKDGNTDKRFDKIKEKYHIPLTIIDNAIDFTSLCKQIFIKK"
     gene            13128..>14337
                     /locus_tag="PK105_05070"
     CDS             13128..>14337
                     /locus_tag="PK105_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61114.1"
                     /translation="MYLKTSIVLAAMVVSFALSSWKLKSPEISMVITAIVGALVAGLG
                     LPVRILVEGLFTNFDLAILFVCASVFINIYSYSGSINIVTRKLVQRVDNKWVLLICMA
                     VFMLIPGALTGAGSVSVFVVGGLVATVVRYMGLSDRKTAAFVFIFAILSAAAPPINIW
                     TMMMTAQANMPYVGFEKLLLIPILIVGAFTIIYLGHGTKPESKEKILAELPEAASDMG
                     WFRILCPPVVLIALILLSLYTPFSIPVLGLPLMFVIAAIAAILCNPKKVSFKEYYGVI
                     AGTIEQIFPLVATVISVGVLINVMAATGVRGLLSITFITLPMILIFFTVLLFCPFAQG
                     CLSYGSAIIFGTPLIFMFNAAGMDTTVICAALSLIFPIGDCLPPSRIVGRVSIETVGY
                     KGSYMSFLKQI"
BASE COUNT         3929 a         3298 c         3340 g         3770 t
ORIGIN      
        1 gggagaaaat agcctgaggc cgccatattt attcagctcg ctgaaatcga gggttgcaag
       61 cacatttgaa cgatcgacct tacgcacatt tctccaaccg aaccagaggg caaatattat
      121 tgcaagcggg aacgttagga gaataagagg tatttcgaaa ccgacataag gcatgtcgac
      181 gcccccaccg atgatcatta cgggaatgtt cacgggcggc gcaatcatcc ccaaaattgc
      241 cgcaatcgaa ataagcgctc ccgcatctat ggtagacata cccattgcaa taaacatcgg
      301 agcaactaca gcaccggctg tcagcaccga tgccgtagaa ctacccgtga tcatccctgg
      361 taccatgatc accagggtca gaagaatcat cagaagcgca ggctgcttat tgaacttctt
      421 gatcactgcc gccgtcaacg cttgcagggc tcccgaatcg cggaagccag tcatgaacat
      481 catggctgtc gcaatcgtga ggattgtatc gagatagcca aatgtacctt ctaccaagtg
      541 ccgaatcgga tcttgtacgc caccgacaat tgctgcaagt gctgaggcga ctagcattgc
      601 aactgacacc ggccatttga ataaaaaaca tccagaagca aatgcgccga ccatcagaat
      661 gaaaagccat aattcctgtg tcacagagac ctcgctattt taggaaagcc gcagccaaag
      721 gctcgccgat ctgtgagatt ttttgcacaa tatcgagagg aatcttattt ccgcacagct
      781 tggtaaaaag gccatcttta ttgccttcct cgacaacaat gaaatagtca caggaaggga
      841 ctgcctcatt tatgaagcga tctgaaagct cgccacgccg agcttcgcca ccgatatgca
      901 ggccgattat cttcataccg cgttttttag cctcggcgag cagggctttt gtccgttcga
      961 gttctgcttc tgccgagata ccggctgcgc ccagtccctt ggatgagcct ccaataacta
     1021 ttaccaacgt cttggcattc gtgctcgcaa gggcttgagc cttgattgtc ggatccatgg
     1081 tgaaatttaa ttttgccctt gccatgatag tctttgccat ttccacatca gcgctctgac
     1141 ctatagaagt gagcaaagct ggctgcggcg caaaagccgc cttgagatta tatgcggcga
     1201 gcggcagtat agctccacct ccaaaaaaca acagaggcac taagagaatt ggaataatcc
     1261 ttcttgcatt tctcatttta attccctttc ttccttagaa tatagtcttt ccaaataaaa
     1321 caaaaataag atctctttat gcgatacaga accaatgaag ctccagagaa atatagactg
     1381 gaacaaagct ctaaataaag ccggcctgct atctcagcgg ccggctctat tgtctaatac
     1441 agtctaatgc gatgcagttt tgccgtgatt attagctcag aaggccgctg cctgcccatc
     1501 tctacgaggg tccgcacctc catagagcaa tccttcgtta taatcataca gcactgcgtg
     1561 cactccgcca aagtaggaat cccagtcgcc gcgaatagta acatcatgcc ctagcttctt
     1621 gattccatcg agtgcgctga ctggataccg cccttcgaga ttcaagcttc cggaagcagc
     1681 ctggaaaagc cttggcgcaa gaatcgcttc ctggatcggg aatccaaaat cgatgacatt
     1741 cgagatgacc tgagcaacag tggggaatat tctcgtcgcg ccaggagatc caattgccat
     1801 aaaagagcgg ccttctggat cgaggacgag agtcgggctc atactggaaa gaggccgctt
     1861 gcctggctct acagaattta cggaaccagg agtcggcaca aagtcgtcca tctcgtcatt
     1921 cattatgaat ccccagcctg gaataacgac gcctgagccg aagaagtagt tgatagtctt
     1981 ggtaactgct accatattac ctgctttgtc catcacagag aagtgagttg tggagcccga
     2041 ttcatatttg aatggatcgc ctggctgggg agagctcatc gatgcattca tattgatcct
     2101 tgtcgcctgc tgcttggcat actccttatt agttagtcct ttgagcggga cctggacaaa
     2161 atcggtgtcg gccatgtact tcgctcggtc tgcaaaggtg agcttcagta tttctgacca
     2221 gagatgagat gcctgcgctg tctggaagcc gagctttaca acatcgaaat tctcaaggat
     2281 attgagaatt tcgataagat gagtgccgcc agaggaggcc ggtggcacgg agtagacctt
     2341 gtatcctcta tattcgcctt ctactgcctt acgaactttt atttcgtagt tagcaaggtc
     2401 ttcaagggtg aggatgcctc cgcgtttctg aacttcatcg accattgcct gagcgattgg
     2461 acctttataa atagcatcag cgccttcctt tacaatcttt gccaatgttc tggccaggtc
     2521 gggattgtat ataacatcgc caacctcata aggaaggccg tcctttaggt agatctgggc
     2581 gccgttctca tatttaacga gcttgccata gttatctttt atgatggatt ctagattgac
     2641 agtgaccggt atgccctgca atgcccactg gattgctggc tgtatgatat cggctcggga
     2701 taatttgcct gagccatagt tttctagtgc atacaataag ccctttacct ctcccggtac
     2761 tcctacagaa agcccgcctt ctatggtcga attgggaata accttgccat ccgggccaag
     2821 atacatagtt ggagtagcct tgccaggagc cttctcgcgg aaatcaatca ctaccggctc
     2881 agccatgtct accattttga tgatcataaa gccgccaccg ccgatgccat tggcatttgc
     2941 ttcgagcaca ccgagtgcga agccggtcgc aatagcggca tcgacagcat tgccgccttt
     3001 cttaagaata tcgattccga cctgggatgc ttcaggctta gctgcggcaa caactccatt
     3061 ttctcctact gccgcgcggc catataagtt gaccggtaac tgggcggaaa tagtagcaat
     3121 tgctgctatt aacaacataa gcgcaagaat agattttttc atatgcgcct cctttcattc
     3181 ttctcttata gtatttagca atttttagct tcttatagtt tttggcaggt gtcaaccctt
     3241 tgtcggtaaa ttgggctcaa tactcacaaa ccgatcgagc tcgatctaat tctcaatccg
     3301 tatatactgc tacaaacctt gtttataaga gttgttaatg gcataagaaa gccttctcta
     3361 aggaaaagaa caggtataga gacgcactct ctatccattc ctaatttatc cagtgttctt
     3421 cccattcgcc gactatagcc gcaacctcgc tagcatatac agaaagcatg cgttccgtag
     3481 atgttttttc ctttatcatg gcggggaaag cctgatgagc gatttcgact atcgatttct
     3541 gtgctatatc tctcgactgt acaaaggctt gttggcttga aagttcgagc ttgcgtggct
     3601 tggaggccca gttttttcgt gcttcccagt aggaatagct tttttcggca ttattctctg
     3661 ttaagagagg tttccagagc gcataaaagt ggccgtcttt caagagaacc ggcttataga
     3721 gagaaacaca ggggaaggag ctatctgtgt accaaataat cgcgccacct ttgcaatact
     3781 ctattaccat agaagctgta gtagcgctct ttgggaaaat accaggatgg atacagacat
     3841 ttttcattcg gttcaggaat gcgcccggtt tcgaagctcc tttgtgcgag cgcagaattg
     3901 caaagaaagg ttctatcgcg ctcgaaaaga gtatttggcc tccttcagtt ttgtctgatt
     3961 ctgccgctat gcgcagaagg ccagcaagtg atgtggatct ccgctgctct ccctgagtaa
     4021 agaaaaggta ttttttatct tccacatatt ttttccagga gccacgctgt tctttgcgat
     4081 cccgatctgt ttcatcagaa caatctccct tatcatttac cagagcaatc tccttgcgcg
     4141 tcatagcatc gatacatgta aagtcgtctt ctatacaata gatattggat attgcgtcag
     4201 caatcatcgc cttccgccat gcccagcgcg gtccggccgt ctcgaggata taggcttcat
     4261 cggggtctgc tataagaaaa ctgttattat aaaaaagctt gcccttatat gctccattgc
     4321 ctccttgcgg gaatctttcg acaaaagaac ttatgaaatt gagagcctct ttagcagtag
     4381 cacagaccga tagcgcagct ctgaggatat ccatgccaag cacgcctttc ttatctggct
     4441 tgaagcgcga aaagactgct tcgttgccaa tagcaagacc tctattattg aggcccattt
     4501 cacctccatc gatccaagaa ggtttggaga gaagaaaatc atagctcata tccggatact
     4561 caattccttc ccctaattta gcaaccttgg gttcttgagc aggaatatag acaagagctt
     4621 gaggttcttc tgggtgtcga tcggaattct tgccgaatat aaagggattt ccccggctat
     4681 tgtgcagaat cagagtatca cacataggct tgcatacctc ctcggggatt aaaccccgcc
     4741 gcaagtcgat gtattatcgt atatcaactt tcaatattcc cgcaagcgca aaagtctgct
     4801 atcctggctt ataatggatt caaataaggc acgctttgat ctgatattac gaatcgcgat
     4861 ttttgttcta gcgatgatga atatctcgaa attaatatcc gtgaatagtc tgctccatgc
     4921 tcagaccaat tcggtcgttc ttaaggaatc ctgccaatcc agtccaccaa aaaacccagc
     4981 tcaaatcggc aagttccaat tcgatagtca ggggtttcta tcttccaacc aggatctttt
     5041 gcctggaatc ccttttccaa agggcatgat gccacccgaa gttgaagcct ttcttgagct
     5101 tcgcgacaac gtgtatgaaa acgctgctcc ggaatctgta gaggcacaag cagtttcact
     5161 gctaaagagg ctaagcagcc tgcctgtatc catctccaac caggcactgc tcgaagcgcg
     5221 tatagcatat ttggttggtc gggcttggaa tgatcataaa gacaaaaaca aggccattcc
     5281 ctggttcgag cgcgcagtgg aggcagctca gagaatggtc aacctggaag gggagacacc
     5341 tacagccctc gtagtgcttg ctgagccatt gggcgagctt agtattctga aagatcttgg
     5401 attcctaatc aaaaatggtc ccaaagtagg ccaatatgcc agtaaggctc tcgaattgga
     5461 tccaaaaaac atcaaagcct ggcttctcaa agcgagtgct ttggcttatc ctccgcctat
     5521 ttggggcgga aattataaaa aagccttgga aacctatgcc agcgttctat tgatagccca
     5581 gaacggcttg cccaaagatg ttctattcga tttacgtgtc ggtatcgcca cggcatacag
     5641 caatctcaaa ctctccgaac atgcaacatg gtggtttgat gccgcgcttc tactttatcc
     5701 caaaaacctc tatgcaaaaa gtgaattgga gaaactgtca ttatgattag cactttgctc
     5761 aatgcttatg ggttaatcgt ctttgccgca tgtgtgtttt tctttttcat cacggtttcc
     5821 aacatcttat ggcttatgca cacactcact atagagcgaa aaaaaaacgg gccatctgta
     5881 gccgtactca tcccggcgcg caacgaagcc ttgcgtattc gcccttgcat cgattctctg
     5941 ttatctcagg attataagtc ctaccagatc tatgttatcg atgacaactc cgacgacgag
     6001 acttggaata tcctgaaaag ctatatgata cgatttccat ccaaattcaa ggcattcaag
     6061 gccgaacctc ttccggaaaa ttggtatggc aaaccccatg cactacagga attagctact
     6121 catgtcaaag aaaactactt gctatgcaca gatgcagaca cgatccataa accagattcc
     6181 ataagcaaag caatggcagc tgcggaaaaa tacaaagccg atctgatcac tggctatgct
     6241 catcatttga tgcccacatt cgcagaagcg gccattgttc cctcaatgta tatccttact
     6301 atgctgggaa tgccactcta cctgattcca aatacaaaat ctccactttt cagccatgct
     6361 atcgggcagt tcatgctttt caagaggtct ttttttgaaa agattggtgg ctatgaaaag
     6421 gtaaaacatg aagcgacaga agatgtgaga ctatcgagga tcataaagga aaatggcgga
     6481 aagattgcct ttatcgatct aaaagacatc gtggaatgcc gaatgtatac gacctataag
     6541 gaagccatcc agggtattgc caagaatgcc tatgattatc tgggcaaaaa cctcttgatt
     6601 cttttctccg gaacagtggc tgtgcccctt gtcttttatg tcccattgat tgtgcttttt
     6661 atcgatgttc catggcttgg gcaggccatt ccttatctca aagcttcggc aattatcaca
     6721 ttttactcct gggttcttga aacaattgat cgtcggctac catggtatgt gccatttatc
     6781 tatccactca tttttgtcaa tacaatgtca gcgctttggc gcggattcca tcttgtaaac
     6841 aaggaaggtg gaatcgaatg gaggggtaga aaagtaaaat gagataccga aaaggggctt
     6901 ctcttgtatc agacacagcc atatcgcgtc tcattggcga tgttctcctg gcatttattg
     6961 gcttgatagc atggccactc tgtcatcttc tatataatat caagatcgaa aaaatcggca
     7021 gggcgaagac agaaaggctt ccatctcgag ctgttctggt gagcaatcac tgcatgcctc
     7081 tggatcccgt ctttcacggt ctttccattt ttcctcgccg cacttttttc actctccttg
     7141 aggaaacatg cgaggcaccg gtgatcggcc ctttagtgag actgctcggt gggattccct
     7201 tgccaagaaa cagaaatagg cttggagata tcgagatggc cgtagagcac gctcttaata
     7261 caagagggct agtccatttc tatccagaag gcgaatgttt tctgggaaat caggatatat
     7321 ttccatttaa ggcgggcgct gcatattttg ctataaagtt tggcgtgcca gtgataccta
     7381 ttgttacgat cttaaagcgc gcaaatggga ggaaatcaaa gcgcatccag gtcaatgtgc
     7441 acatgctcga gcctatcatg cccctggctt tgctagacaa gcccctatcc ctatcgagca
     7501 tggccaaaaa tccagcccaa gaaaattcac gcgagatgct ctctagagca atcgagtgca
     7561 cacgaaagtt ccatgctctt atgcaggcag aaatagattt ttctggttgc gacaagtcac
     7621 tctacaaggg acctatgccg cgaataaaag gagtgaatga ttagtgctgc tacgaacctt
     7681 acaaggttcg tagcagtatg ggcatgcagc cattgcataa tcagatctta tctattctct
     7741 acaatatacc taatgccgat tatctaatat gataggtatt tctttcttat gattatgtta
     7801 caaagatgca ccggtgcatt atatatagat attgcaatat agggatggtt tggaaatgga
     7861 aagattcttg gagcgtagtg gatacgcaga ggatcgaatt cttcaattct tcgcacgcct
     7921 catcggtgat cagtttttcg ttcagaaaaa agacatcctt gtacacgatg aagacttagg
     7981 ttatcgatcg ccactcatca ctgctcttat cgagagctac atcgaggatt ccaagacctc
     8041 tgcgacctcc aaaatatctg gaagcgaaga agagacgcga gagaagcaag ccagaatacg
     8101 agccttagtg ctcaccgagc atgaagaagt attgatcgat ctgcagaagc tatttgcaat
     8161 tgctagtttt gatagtttgg tgatatcgtc ggatacagcc ctgaaagaag caaatatgcc
     8221 tctctccgag aggacagtag ttgcaatgcc tacatcgctg tttctggctg cgacacaagt
     8281 gcctgaattc aaggcgcgcg aatttggcct tgtcgtgctc gagggagccg acctattcgg
     8341 agacacgcct ggcgaagtgc agcgtagaat atggggatat ctccttccac catggcagcg
     8401 gagggcagcc atatttgcca agcatatcgg gataaggaca aggaatacag cgctggattt
     8461 tgcaaatgat cccaagacga tcgtattaaa aaaagcccaa gcaagcttaa gccaaataag
     8521 cacctatatg tatcgtctaa gttcgcaaca taaattcagg gtgctacttg caatgattca
     8581 agagaatttt caagaagata gaagagctat tattgtattc tgcaatttga gacagacatc
     8641 gatggagctg gaattcaggc taaagctcaa taatatcaaa gctaagcaag tatccatttc
     8701 ggctccgaaa gcagaaagaa aggagcttct cctggaattt gccgagattg agaagaaagc
     8761 agaaggaggc caattcgttc ttgttgtctc caatgataat ctgagtgtgc tgccttccga
     8821 attggcaaat ctagctattc attatgatat ccccttagac cctgatatat atcttgagcg
     8881 agtgcgaata atgcgaccaa gtagctcgac cataataggc ctagtatgcg aacgctatga
     8941 agtcggctta tcagcaatta attccaaatt tggcatgcaa ttcgaactaa gagagcccag
     9001 ctatgacatg ttggaatacc gcgatgagag cgaaggggta ccattgaaaa tagaatatga
     9061 aagacgatct tcggaataca aaccgagacg caggattgag caaagcaaag ataaaacgga
     9121 agatgcaaag aggattaaaa agactcaaca tagagaatta aaggaaaatt tccatcaagg
     9181 tcaaaaagcc caacataata gaaaaaacag ctcgcctaag aaaagtccag gagtagacca
     9241 gaatagcgaa ctctattcca tgagcaccga tgagcgcttg gcttatttcc gacagaaata
     9301 ccgtggcatt ctgaattcgc ctattgaacc aaaaccgcat cccaaaaaag gagaaaaaac
     9361 gatatccagg tcagcatcag agccattgtc tttcgaaaat acaattccta ctaagaatgc
     9421 agatggagag cgatcgatca gcgccgcgga ctcaattgag gttagaaagg gagataagaa
     9481 acaggctagt ggcatggaat cggataatcc aagtatagta aaacggttca tagaaaaact
     9541 gttcccagga acgaacgaag gaacagatga aggaataaag gaatagatga aggcgcaaat
     9601 tagcgagcag acaagcgaac aaatgcttga gtcatccttc ccggacatgg cctttcctga
     9661 gtccgatagc aacaaacaat aggaaagcag aaacatgaga gcagatagac cagaccggga
     9721 aagtgaaggc aggaatccta ttctaaaaac gaaaagaggt atttataaat gaaaatattg
     9781 tcgcatacgg tcaaaccagc gcttaaaggc cgtctgacca aactcgattt aattgcgcga
     9841 aacctatggc tttcgtggaa ttttgatgca gtgtcactct ttactcgcat cgatcgaggg
     9901 ctgtggtcgg cctcggggca aaatccggta agaatgctgg gcatgctgag tcaggaaaag
     9961 atcgacgagc ttgcctccga ccctgctttt ctctcggacc tggatgaggt ttattctaat
    10021 ttccagcgat acctaaagaa cagaatctgg tatcgcggac cacgcgatag agtaattgcc
    10081 tatttttcta tggaatatgg cctcgatgtc tccttggcga cctattctgg cggactaggt
    10141 gtgctctcgg gtgatcatct aaaaaccgtg agcgatctcg gattaccgct agtggctgtt
    10201 gggcttcttt atcgtcaagg ctatttcaag caatatctga atgccgatgg ctatcaacta
    10261 gaaacatacc ctgagaacga ctggtacaat atgccggtgg agcgctgtgt ggatgcccag
    10321 gatgcacctg tgctcatcca ggtcgaaatg gcaggccgcc caatcagcgc cggaatctgg
    10381 cgtgtagata ttggaagagc tcagctttac ctccttgaca caaatattcc cgagaataag
    10441 ccagaagatc gaatcatcac agcgacactt tatggtggcg acatggaaat gcggataaag
    10501 caggagatat tgttaggcat aggaggaatt agggcactca aggcactcgg tatccatgta
    10561 ggtgcaaccc atatgaacga agggcattcc gcattcctag ccctggagag gattagagta
    10621 ctcatggaag aatatggcct ggatgcacat tcggctattc aagcatttat acctacgaat
    10681 gtcttcacga cacatacgcc agtacctgca ggtaacgagc gcttcggaat agagttaatg
    10741 gagaagtatt tccgctcttt tgtaactgcg ctaggcttag actggaacga attcttaggt
    10801 atgggccgcg agaatcccct ggacaaacag gagagtttct gcatgactgt ctttgccctg
    10861 cgtttgtccg ttcgatcgaa tggcgtgtcc aaattgcatg gcgaagtctc gcgcagaatg
    10921 tggaaaggct tgtggccaga gctggatctg aaagagatac ctattggaca cgtaacaaat
    10981 ggggttttcc cccgaacctg gatcagccat gacatgctca gtcttcttga taaattcctt
    11041 gggccaaaat tcgagctgga gcccgacaat cctgatgtat gggctgcaat agatcaggta
    11101 tccgacgaag agctatggag aacacatgaa cgtcggcgag agcgccttgt cgcaataagt
    11161 cgccagcgcc ttgccgcgac atataagcgc ctcagtctac ccgatggcga gatcgtccgc
    11221 tccagcgatg tgctttctcc ttatgcgctc acattgagtt ttgctcgtcg ctttgcaaca
    11281 tataagcgag gaaccttgct tctcaaagac cctgatagat taataaggct tcttaacgac
    11341 aaggataagc ctattcaaat agtaatagcc ggaaaagcgc accctcatga tatggctggc
    11401 aaagagctta ttcgagaaat catacatttc tctcggcgtg aagaagtctt tggtcgcctt
    11461 gtgtttatag aagactatga tatggcgatg ggtcgctaca tgacgagtgg ctcagatgtt
    11521 tggctcaaca cgccgcgccg tccacaggaa gcgtccggaa cttccggcat gaaggctgga
    11581 atgaatggcg tactcaactg ttcggttctg gatggttggt gggctgaggc ctatagttcc
    11641 gagatcggct gggctatcgg ttctggtgag gaatacaagg atgaagctct tcaggatgag
    11701 atagaagcag aagatctcta cgacctgttg gagcatgaga ttttgccgct cttttatacc
    11761 agagggcgag ataacattcc gcgaggctgg ctcacgaaaa tgcgctcttc gatgaagacc
    11821 attggctcta agttcgcaac gcaccgaatg ctcaaggaat actataccaa ctactatgag
    11881 cctgctctta gggagagtaa aacactggag gccgataatt atcgcgccag cgtatccctg
    11941 gctcagtaca tagaaaaagt caaacgtgtt tggaatggag ttcggatcgt cgaattcagc
    12001 gatgatggag ctcccgttgt gccaagagga agcgccatcg aagtgagagc tctcgtcgac
    12061 cttgctggtc ttctgccaga tgatgctgcg gtcgaatgct acttaggcag actctcgagc
    12121 aagggagaaa tcctggaggg aacgcgcata ccaatgaccc tattgggacc tgaggggcaa
    12181 tactatcgat accaatgtga cataaggagc gagataacag gccaaatagg ttattcagtc
    12241 cgcgttttgc cggcgcatcc cgctttagac ggtcgttttg tgccaggcct tgttcgctgg
    12301 gctcaatagg cagattaagg aaaaacgaat aaatgagaat atcgcgttga caaaagcgaa
    12361 gtctgctcat agaattctat attgccaggt gtaaatcgac tggtctaaca gattttgatt
    12421 tggttttcag gtaactccaa taattcaaaa aaccaaacga aaggaattat atatgagaaa
    12481 aggatttttt atcctgttgt tggtggcttt gctgtcctgt atcgggccct tttcggtttt
    12541 tgctgcgata agcggtgatc ctataaagca atatgggctt cctttcctta tcacaaacgc
    12601 agggcaaggg ccgggtggca aaatggcgcg tcttttggtc tcgcagacta agacgctgga
    12661 attagataag gatttcttct acttggccga accggttgaa gcagatctta aagcaagacc
    12721 atataaggct cttatggtgg tcataggatc ttcggacaag ggtctaggtg cttctggaat
    12781 aaccatcgat caagagatag caaggctgca gcgaatagta aaggcgtcga aagatatgaa
    12841 gcttccgatt atcgctgtgc ttctcgagaa agacaagcgt tcgaatgttg ctacaaaccc
    12901 aaacgagcgc tgcatagata cgatttgccc ttatgccgag tggatgattg ttgtaaagga
    12961 tggcaatact gataagcgct tcgacaagat caaggagaaa tatcacatac cattgaccat
    13021 catagataat gcaatagatt tcacctcgct ctgcaagcag atcttcatca aaaaataata
    13081 agagctcgta tcaaactcgg gcatcagctt gataaagaag gataactatg tatcttaaga
    13141 catcaatagt tttggctgcc atggtcgtct cattcgcgct ttctagctgg aagctaaaat
    13201 cacctgagat ttcgatggtg ataaccgcta ttgtgggtgc gctggttgcg gggcttggct
    13261 tgcctgtacg gattctcgta gaaggactgt tcacaaattt cgatctcgct atcttgtttg
    13321 tctgtgcgtc agtattcatc aatatctatt catattcagg gtcaattaat atagtgacaa
    13381 gaaaattagt ccaacgcgta gacaataagt gggtactcct gatctgtatg gccgtcttta
    13441 tgctaattcc gggagcgctg acaggagcgg ggagcgtctc ggtatttgtg gttggcggtc
    13501 ttgtggctac ggttgttcga tatatggggc tttcggacag aaagaccgcg gctttcgtat
    13561 tcatctttgc cattctctct gccgctgctc cacccatcaa tatttggacc atgatgatga
    13621 ctgcgcaggc aaacatgcca tatgtgggat ttgaaaagct tctgcttatt cccattctga
    13681 ttgtaggtgc atttacaata atctacctcg gccacggtac aaagcctgaa agcaaagaaa
    13741 aaatacttgc ggagcttcct gaagcagcat cggacatggg ctggtttaga attttatgtc
    13801 caccagttgt tctaatagct ttaatacttc tgtctttgta cacgcctttt tctataccgg
    13861 ttctggggct tcctctaatg tttgtgattg ccgcgatcgc cgctattcta tgtaacccga
    13921 aaaaagtatc gtttaaggag tattacggcg ttattgcggg caccatcgag cagatttttc
    13981 cattggttgc tacggtaata agcgttgggg tacttataaa cgtaatggca gcgaccggtg
    14041 tgcgcgggct tcttagcatt acttttataa cgctgccaat gattctcata ttcttcactg
    14101 tgctattgtt ttgccccttt gcacaaggat gcctcagcta tggaagcgcg atcatattcg
    14161 gtactccgct tatttttatg ttcaatgcag caggcatgga tactacagtg atatgtgctg
    14221 cgttaagcct catcttccct atcggcgatt gtcttcctcc ctcgagaatt gtaggtcgtg
    14281 tctccatcga gacagtgggt tacaaaggct cttatatgtc tttcctaaaa cagatat
//
LOCUS       DAOOWY010000047        13830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4455_length_13830_cov_14.906134, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000047 DAOOWY010000000
VERSION     DAOOWY010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 13830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13830
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4455_length_13830_cov_14.906134"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PK105_05075"
     CDS             complement(<1..362)
                     /locus_tag="PK105_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61115.1"
                     /translation="MRYLYSTMLDNEQEKDNEGGTQDSKSNLSHLFRIALGFAVVSAL
                     ILIYLLVPRSGPAPRILAGGLASGPSMIVQELSVLRSAYPNIKIENIFDPIKSDWKII
                     ISKDDKTMELYWAEGRYLP"
     gene            complement(847..2235)
                     /locus_tag="PK105_05080"
     CDS             complement(847..2235)
                     /locus_tag="PK105_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU61116.1"
                     /translation="METKTEVARTPSFLNAQTPAEMRRVILKLAMPAMIEFGLMMLVQ
                     IIDMIQVGVLGPWALAATGLVAQPTMLIFSVFSALVVGTTALVARAAGAGDSEYASRV
                     TRQSFVIVITMSLITTAVCIPASGSIVRLMGADIDTIEPATIYMKIVMAGTIFTVGNM
                     VLAAALRGAGDMVTPMISNLIANAFNLFGNWVLINGKLGFPRLEVAGAAIATTFSRVI
                     AFSITLHAVYRRDRFIHISIHDSYKVEPEILKKVLTIGLPAAGEQLIMRGGALLFARL
                     IAGFGTIMFAAHQIVINIDGLCLVPVMAFQMATTTLVGQGLGAKNPEFSEKATRQSTI
                     VSVSIMMLIGVLLFFFGNYAVFVFTRDKQVIEFGANAIKILAISQPIAAVFSVLGGAL
                     RGAGDTKYTMYISAIGVWVVRLGLGYLLADALGLALIGAWIAVVADMLVRALLAQRRF
                     SSGNWHKDIKVG"
     gene            2890..3579
                     /locus_tag="PK105_05085"
     CDS             2890..3579
                     /locus_tag="PK105_05085"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOU61117.1"
                     /translation="MKTIGLIGGMSWESTVTYYSIINSRINAELGKNHSAQCIVYSFD
                     FQNIEELQYAGKWAELEVELIKAAKALKAAGADFLVICTNTVHKVADNIEKEVGLPLL
                     HIADVVGEAVQTDGRQRIGLLGTIFTMEEDFYVRRLESKFGLDVLVPEKNDRQVINNI
                     IFSELVKGIVKERSKNEYLRIMDILKNQEAEGIVLGCTEIGLLIKEYDLPLYDSAVLH
                     ANKAATMSLIP"
     gene            complement(3775..4998)
                     /locus_tag="PK105_05090"
     CDS             complement(3775..4998)
                     /locus_tag="PK105_05090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU61118.1"
                     /translation="MNKLKDKLITGIFNDSFAPITDGVAMVTKQYALWLNRKAGPSCV
                     VTVDSSDYYDNEEFPVYRIPSVALAARPPYRAGITILGELAEKRLRGGILKEKLTINV
                     FDIPFDLVHSHCPFASGMLAQNIARRRDIPLVTTFHTKYRPEFEQALKVKPLVAIAMQ
                     YVREYYKRADFVWVPNKETIPILRDYGYSGDLEVMPNGTDLFADVSEIPLLRQEGGVL
                     LNVPDDRFVFIYVGQHIPEKNVDLIIEALNVLNQRGIPFKMVFIGDGKYRGAMEKRVG
                     ELGLSQKVEFRGIVRDREVIKRAYARADLMLFPSLYDTSSLTIKEAAALNLPAVVIRN
                     SPTAEGIVDGQNGFLGDNDQRDYAETIARAIQNESLRKAAGEQARSTLYSSWESVIDI
                     VQNRYAEIVRSWHRR"
     gene            complement(4995..6098)
                     /gene="glf"
                     /locus_tag="PK105_05095"
     CDS             complement(4995..6098)
                     /gene="glf"
                     /locus_tag="PK105_05095"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HOU61119.1"
                     /translation="MKYFEALVVGAGLAGSTAARILAEQGRKVLVVEKHRHIAGHCHD
                     ARNEAGITIHTYGPHIFHTNNKTVWDFVHRFTEFRYFQHRVLSYVDGRFIPFPIQADT
                     INELFGTSIATQEVSDFLAEEVRQSKFQTLPATFRDVVVSQVGERFYSLFFENYTRKQ
                     WGLDPTLLSAEVASRIPVRTNRDGRYFSDRYQGIPAHGYTAMVDEMLNHPNISVLLGL
                     DWFNIKDEIAADLTVFTGELDQFFDYKHGKLEYRSLDLVYQTFDQERYQPAAVVNYPN
                     DYDWTRITEYKYFLEEKSPKTTVCFEYPKASGEPYYIVMTKDNMEKRALYMQEVETLE
                     SQGHWRFIGRLAEYKYYNMDQVIAAALQKIAGV"
     gene            6933..7274
                     /locus_tag="PK105_05100"
     CDS             6933..7274
                     /locus_tag="PK105_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOU61120.1"
                     /translation="MNEDAGGNVPQVADEGGCVSCRYKNTPREEQTLKQLQNRLNRII
                     GQLNGIKTMLDDNRYCGDVLTQVAAIESALQSLGYIILRNHLETCVVEEIKKDNMQIL
                     DEALELIRKLK"
     gene            7309..9747
                     /locus_tag="PK105_05105"
     CDS             7309..9747
                     /locus_tag="PK105_05105"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOU61121.1"
                     /translation="MTKFSVTGMTCAACSAHVEKAVAAIPGVKSVSVSLLTNSMSVEF
                     ASPASAESICAAVDKAGYGAAPEDVDSGLSAADAARKRASERDALDDHETPRLVKRLI
                     ASLILLVPLLYVSMGHLMWKWPLPAVFSENPAAIGLYELLLTLAIMMINQRFFTSGFK
                     SFVHGAPNMDTLVALGSGASVVYSVVVLFLITGAMLSGMLHDAHEYLHDLYFESAAMI
                     LTLITVGKTLEAYSKGKTTNAIKSLIDLAPDTAHVIRDGKELTIPAEQVRTGDTFIVR
                     PGERIPVDGKVISGTSAVNESALTGESLPVDKSAGSDVSAATLNQNGFLTCTATKVGS
                     DTTLSQIITMVENAAATKAPVARLADKVSGIFVPIVIVLSVITGIIWAVAGGEFGFVL
                     ARAVSVLVISCPCALGLATPVAIMVGSGLGAKHGILFKTAAALEAAGKTDIVVLDKTG
                     TVTEGKPTVTDVIPADGVTERELLALAGALEEKSEHPLARAVLEKVRAENIAYSPAED
                     FTALPGYGVKGVVPPSGNDSGAVAPVAGVPASGGNAALMKDQRLLGNEMKQRGMELAG
                     EGKTPLYFAAGGKMLGIIAVADVAKKDSADAVQKLNALGVQVVMLTGDNRRTAEAIAK
                     QVGITHVVSDLLPGGKDAVVAKLSAFGKVMMVGDGINDAPALTRADTGVAIGAGTDVA
                     LDAADVVLMKSTLVDVPAAIKLSRQVLTNIRENLFWAFIYNIIGIPVAAGALIPAFGI
                     VLNPMLGAAAMSLSSFCVVTNALRLNLFNMYKSGRNGKRKTIQLPDDIFDGRNISHNI
                     QNLPDNFVTQSPHV"
     gene            complement(9756..10997)
                     /locus_tag="PK105_05110"
     CDS             complement(9756..10997)
                     /locus_tag="PK105_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61122.1"
                     /translation="MGKLKHIIRHEFRLTTANKAFVILTIIGPFLIFAITVLPTLLAQ
                     SSSPVASDKPLALFGAPPRVEQAISAAFSGMKLQVEHVEDIEAAKASVIDGSRVALLE
                     LEAGWPDTGKAIWYSKSSAEIGLYSTASAVLEAVAREERIRESGIDPALAAKLLSKPT
                     FEVIKLEKGGEEKAQGQGDQAFLETLMTVLVFIMLLYMTILLYGQMIGRSVVTEKTSK
                     TVEIMLSSVTSRDLMIGKILGLGLAGMLQYAVWISMAFVLNHFLGPLFGLALPVAVSM
                     QNMMWLFVFFILGFFLYSSAYAALGAASEDEQHLGQIAWPLLMFLIIPMVMLNTLVTS
                     PDSTAVVIMSFFPLTSPIVMLSRILVASPPVWHLVLCIGILVASIVGMMVFAGKVFRV
                     GILMTGKRPKLKEVLRWVKVK"
     gene            complement(10990..11847)
                     /locus_tag="PK105_05115"
     CDS             complement(10990..11847)
                     /locus_tag="PK105_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU61123.1"
                     /translation="MEGLSFEAVPGEIFGLIGPNGAGKTTTIRMIMNIIVPDSGIIRF
                     NGAPLSEADKDHIGYLPEERGLYRKAKLGEVLEYLAAIKGAKANEYRLSINAWLERFG
                     LSEWKNRKISDLSKGMAQKAQFIATVAHNPSVVLFDEPFSGLDPMAQDDLLSVMVELK
                     HKGAIVLFSTHIMEHAEKICEKILLMNKGKAIVYGSMADIKAQYGRNAVQLEFDGDAS
                     FVQSLDFVDSVVSYPRWIEVELKEGTDSDLLFKALAGRLKVRRFETVAPSLHKIFIHL
                     VGGEEIENG"
     gene            complement(11917..12090)
                     /locus_tag="PK105_05120"
     CDS             complement(11917..12090)
                     /locus_tag="PK105_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOU61124.1"
                     /translation="MEGLEKDNEKKQILLRLSPTLWKELAAWAADDFRSINGQIEYLL
                     TECVRQRKKKKIE"
     gene            complement(12201..13217)
                     /locus_tag="PK105_05125"
     CDS             complement(12201..13217)
                     /locus_tag="PK105_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU61125.1"
                     /translation="MNQSPLNEEKSAPSLSGMAMLILDVFLMIVTVAGFVFGTSSGKT
                     DAGTIVLMVVCGLYAFLIGPILFAGLKVLKPNEAMVLTLFGNYHGTLRGPGFFFVNPF
                     VASVCPSNVQLSSGSIPLPIGETGSSSAAHKTHGASSKQASKKISLKAMTLNNEKQKV
                     NDALGNPVIISIVVIWRVVNTAKAVFNVDNYFEFLSIQCDSAARNIVRLYPYDASEEE
                     GEKSLRGSCQEIAESLKEEVQKKVDMAGLEILEARITHLSYAPEIAAAMLQRQQASAI
                     IAARQMIVEGAVGMVEMALAKLSDRNIVNLDEERKATMVSNLLVVLCANKDPQPIVNT
                     GSIY"
BASE COUNT         3701 a         3361 c         3212 g         3556 t
ORIGIN      
        1 ggcaaatacc gtccctccgc ccagtaaagc tccatcgtct tatcgtcttt gctgatgata
       61 attttccaat cgctctttat tggatcgaag atgttctcga tcttgatgtt aggataggca
      121 gagcgaagga cactaagctc ttgcactatc atggaagggc cgctggccag gcctccggcc
      181 aggatcctgg gtgctggacc tgatctgggc acaagcaagt atattagaat gagtgcggag
      241 acaactgcaa atccgagagc gattctgaat agatggctca gattggattt tgaatcctgc
      301 gtccctccct cattatcttt ttcctgctca ttatcaagca ttgtgctata aagatagcgc
      361 atctgccttg cgctatgtca agagagcaga tttctttcat gtcaaaatct tttgattagc
      421 ggcagaactt tgtaacacaa tcgcttcatg catagatcaa cccctatttc gcctttaggc
      481 tgcgcaatag ctttttgatt gatacgtatt ctattttttg cacattttga gagatcgaaa
      541 aaggacgccc tattctggtt aagttcaatg accagaccaa gcttgaccaa gaaggggtga
      601 cgccatgaac ccaattggag aaagccaatg cggtcgccga gaaactcaaa acttatgctt
      661 gtcgttgacg cctgaaatac atctcgggac gaaaattgaa tacgctgcga tacatgaaca
      721 tggatctctt attcaaagaa acaatcggct accatatcct agatgctcag cataaagcca
      781 gatgagggcg acctgggaaa tgtgctataa gattgttgac aataccaata aatacggcct
      841 ctaattttac cctaccttaa tatctttgtg ccaatttcca cttgaaaatc ttctttgggc
      901 aagcagtgct ctgacgagca tatcggcaac aacagcaatc catgcgccta tgagtgcaag
      961 accaagagca tccgccaaca aataaccaag tcctaaccgt acaacccaca ctccaattgc
     1021 acttatatac atggtatact tggtatctcc ggcaccgcgt aaggctcctc caagtaccga
     1081 aaaaactgca gctattggtt gagatattgc tagtattttt attgcgtttg ctccaaattc
     1141 gatcacctgt ttatctctag taaaaacaaa tacagcataa ttgccaaaaa agaataacag
     1201 cactccaata agcatcataa tactaacact aacgattgtt gattgacgtg ttgctttttc
     1261 agaaaattct ggattttttg cacccaaacc ttgaccaaca agtgttgttg ttgccatttg
     1321 gaaagccatt acaggcacga gacacaaccc gtctatattt attactatct gatgagcagc
     1381 aaacattata gtgccaaatc cagcaattaa tcttgcaaaa agcaatgctc cacccctcat
     1441 aataagctgc tctcctgccg caggaagtcc gatagtcaaa acttttttaa gtatttcagg
     1501 ttcaacttta tacgaatcat gaatggaaat atgaataaat ctatctctgc gataaactgc
     1561 atgaagcgta atagaaaacg ctataactct tgaaaatgta gtagctattg ccgcgccggc
     1621 cacctccaat cttgggaacc caagctttcc attaataagt acccaattgc caaatagatt
     1681 gaatgcgttt gcaatcaaat tagaaatcat cggtgttacc atatcgcctg cacctctcaa
     1741 tgcagctgcc aacaccatat tgccaacagt aaatattgtg cctgccatca ctattttcat
     1801 ataaattgtt gcaggctcaa ttgtgtctat atctgctccc attaatctta ctatgcttcc
     1861 tgaggcggga atgcaaactg cggttgtaat aagtgacata gtgatcacta tcacaaagga
     1921 ttgccttgtc acacgcgaag catattcact gtcgccagca ccagccgctc tcgcaacaag
     1981 ggcggtagta ccaactacta acgcactaaa cacggaaaat attagcatgg tcggttgggc
     2041 aactaaacca gtagcagcaa gagcccaggg ccctaagact ccaacctgaa tcatatcaat
     2101 aatctggacc agcatcataa ggccaaattc aatcattgca ggcatagcca atttcagtat
     2161 tactcttcgc atctcagcag gagtttgagc gttcaaaaat gacggcgttc tagctacttc
     2221 agtttttgtt tccattaaat ctccatactt atactcatta aattcgttta tctcttcgta
     2281 tactgcagaa ttaagtttgc aatttggtag ttgaagaagg tcattggaag ctccagaatg
     2341 ttgttatcta aactaacaaa aaaaggacaa ccaacgacca aggccaaaga tagcacattc
     2401 tttgaaaata ggctattgga tttcattgca gaacgagacc cgctattttt cgtgatttag
     2461 tggctcattg aatacctcgc gattgatgtc tggcatcatg actatggtcg gctcaagatc
     2521 gaatcggaat gaatcctctt ctgttaagcc aatctcgccc catgctggct ttatttttcg
     2581 aagactccga gcgcaaaacc ggcctttgca agatctgcgg cagcggctgg cccgcccatt
     2641 cctcaaccca caacgatagc gcttttcact gttttcccat tcttgtcgtc cacattcgtt
     2701 ttccttaatt ataataatta tatatagaaa aaagctcatc aacatcaggc tgtctaaaca
     2761 gaattgtgcg cactcacaag tggagatcgc cctcaacctg cgaagtaccg caacatcctt
     2821 ggccttaaaa ctattgactc tagccccttt ggttctatta taggggagtc tgactagaag
     2881 gtgataaata tgaaaaccat tggtttgatt ggcggcatga gctgggaatc gacggttacc
     2941 tattattcaa ttatcaattc gaggattaac gccgagctcg ggaagaacca ttctgcccag
     3001 tgcattgtct attccttcga cttccagaac attgaagagc ttcaatacgc gggcaagtgg
     3061 gcggaacttg aagtggaact cataaaggcg gcaaaagcgc tcaaagcagc cggagccgat
     3121 tttttggtga tttgtaccaa taccgtccac aaagtggccg acaacatcga aaaggaagtc
     3181 ggcctgccac ttttgcacat tgccgatgtc gttggcgagg ctgtccaaac cgatggccga
     3241 cagagaatcg gtttgcttgg tacgatattc accatggaag aagatttcta cgtaagaaga
     3301 ctcgaatcga aatttgggct ggatgtcctc gtgccagaga agaacgaccg acaggtcatc
     3361 aacaacataa tcttttccga gctcgttaaa ggcatcgtca aggaaaggtc caagaatgaa
     3421 tatttgcgca taatggacat actgaaaaac caagaggccg aaggcattgt cttgggctgt
     3481 acagaaattg ggcttttgat aaaagaatac gatctgcctc tttacgattc cgccgttctg
     3541 cacgccaata aggcagcaac tatgagtttg ataccttgaa ttcttgaagc tcgaaacaat
     3601 gttctggggt tgggctggtc catgaaccaa agcctagccc attcccctgg gagaaattga
     3661 aattgaagta ggtacactga tgagactttg gaccttactt ccatgttatc ttgttatctt
     3721 gaccaaaaag gccttactgc tgtgcggcgc gagggtttgc ttgccatggc acgcctagcg
     3781 cctatgccat gagcggacaa tttcggcata tcggttttgt acgatatcga tcacggactc
     3841 ccaggaagag tacaaggtac ttctcgcttg ctcgcctgct gccttgcgca gcgactcgtt
     3901 ttgaatcgcc cgtgcaatag tttcggcata atcgcgctgg tcgttatcgc ccaagaagcc
     3961 attctgacca tcaacgatac cctcggcggt gggcgaattc cttattacga cggccggcag
     4021 attgagcgcg gccgcttcct ttattgtcaa gctcgatgtg tcataaagag aagggaagag
     4081 cattagatct gctcgggcgt acgctctttt tatgacctct ctatcgcgca caatgccgcg
     4141 gaattccact ttctgggaaa gacccaactc accgaccctc ttctccattg ctccacgata
     4201 tttgccatca ccgataaaga ccatcttaaa aggaatacct ctttggttaa gaacattcag
     4261 cgcctcaata atcaggtcta cattcttctc tggtatgtgc tgtccaacat agataaaaac
     4321 aaacctgtcg tccggaacat tcagaagtac cccaccttct tgtcgcaaga gaggaatttc
     4381 ggagacatcg gcaaaaaggt cggtgccatt cggcattacc tcgagatcgc ccgaatatcc
     4441 ataatcgcga agaatcggaa ttgtttcttt atttggtacc cagacgaaat ccgccctttt
     4501 gtaatactcg cgcacatact gcatggcgat tgcaactagg ggtttgacct tgagcgcttg
     4561 ttcaaattct ggtcgatact tggtgtgaaa ggtagtaaca agtggaatat cccgacgcct
     4621 cgcaatattc tgtgctagca ttccagaagc gaacggacaa tgcgaatgca caagatcgaa
     4681 gggtatgtcg aagacgttta tagtgagttt ttctttcaag attccgccgc gtaagcgctt
     4741 ctccgcaagc tcgcccagaa tggtgatccc cgctcggtag ggcggccgag cagccaaggc
     4801 taccgaaggt attctataga cagggaattc ctcgttgtca taataatcgg atgaatcgac
     4861 tgtgacgacg caagaaggac ccgcctttct attgagccac aacgcatatt gcttggtaac
     4921 catggctacg ccatcggtga ttggagcaaa agagtcgtta aagataccgg ttatcaattt
     4981 gtctttcaac ttgttcacac tccagctatt ttctgcaaag cagccgcaat tacttggtcc
     5041 atattgtaat acttgtattc tgcaagccgg ccaatgaagc gccagtgccc ttgggattcc
     5101 agagtctcga cctcctgcat gtacagagcg cgtttttcca tattatcttt tgtcataacg
     5161 atataatatg gctcgccaga agcctttggg tattcgaagc ataccgtggt cttaggtgat
     5221 ttttcttcga gaaagtactt atactctgta atgcgtgtcc aatcgtagtc gttgggataa
     5281 ttgaccacgg cagcgggttg gtagcgctct tgatcgaaag tctggtaaac gagatcgagc
     5341 gatcgatatt caagctttcc atgcttatag tcaaagaatt gatcgagttc tcccgtaaaa
     5401 acagtaaggt cagccgctat ttcgtccttg atattgaacc aatcaagccc caagagaacg
     5461 ctgatattgg ggtgattgag catttcgtcc accattgccg tgtagccgtg cgccggtatg
     5521 ccctgatatc ggtccgaaaa atagcgacca tctcgatttg tccgtaccgg aattcgcgaa
     5581 gcaacttctg cggaaagcag tgtcggatcc aggccccatt gtttgcgtgt gtaattttca
     5641 aaaaaaagcg aataaaatcg ttctccgacc tgggacacta ccacatctcg aaaggtagcg
     5701 ggaagagtct gaaatttcga ttgccgaacc tcttccgcaa ggaaatccga aacctcttgg
     5761 gttgcaatgc tcgttccaaa aagctcgttg attgtgtccg cctgaattgg aaatggaata
     5821 aaccgaccgt ctacgtaaga aagcacacga tgttgaaaat agcggaattc ggtaaaacga
     5881 tgcacaaaat cccacacggt cttgttattg gtatggaaaa tatggggccc ataggtgtga
     5941 atggtaatcc ccgcttcatt cctggcgtcg tggcaatgtc cggcgatatg tcgatgtttt
     6001 tcgaccacca gaaccttgcg gccttgttcg gccaaaatac gtgccgcggt gctgccggca
     6061 agcccggcac caacaacaag agcttcgaag tatttcatcg atcctcctta gataatacta
     6121 atcacaaaag ccgtgcagat ccagctcata agatctttca taaaaaatgt ttaagagcgg
     6181 taacaaaaaa tctttattca taggaactgt catttctgga attttgcggt ttttggatgg
     6241 aattcgcgtt tgccccgaac tgcaaccgat cccgctaccc aaaggactgc tccttccaaa
     6301 tttttcggta acgccagcac aaaaaataaa tctagtctgc ttctatcgtc aacccttgtt
     6361 ttagtacaat tcaaaaaatc gcatgaatac ggctagcgcc accaccgact ttgtcctctt
     6421 gtctccttgt ccccttgcat tttggccaga caattggatc cacttcgtag accttctgca
     6481 aaagtcgtgc ccgactttgc ctgcgaagct tcgcccaagt atctgggact tctacagatt
     6541 tcgcatccat ccttggcatt tccgtagtta tttgggactt agagagatgg ccttgctgcc
     6601 agagataaaa actccgagaa caagaaaaat ggcaacttgg acacttctcc gaacaggcgc
     6661 ttgttgtatt tgaagtagga ccttggaatc tctggtttgg taaacacaaa cttcattttg
     6721 tacagatgcg cctattaaga gttgttcggc gaccgggaat gcatagggtt cggcagagaa
     6781 tgccggcgtg ggtggagccg taatgctggt ggggaatacg ccttttggga cgtcttttct
     6841 tttgtacttg atagataccc ctatggggta ttatatttat tctaaacgaa aagatacccc
     6901 atgggggtat ataaaagaca ggaggcagcc aaatgaatga agatgctggt ggaaacgtcc
     6961 cgcaggtagc ggatgaaggc ggttgtgtga gctgccgcta caaaaacact ccgcgcgaag
     7021 aacagacgtt gaagcaactt cagaacaggc ttaaccgtat aatagggcag ctcaacggca
     7081 taaaaacgat gctcgacgac aaccggtact gcggcgatgt gctcacgcag gttgcggcca
     7141 ttgaaagtgc gcttcagagt cttgggtata tcattcttcg gaaccatctc gaaacatgcg
     7201 tcgtggaaga aataaagaaa gataacatgc agattctcga cgaagccctc gaactgatta
     7261 gaaaattgaa ataggaagta ataactttca aggatggtag atatacatat gacgaaattc
     7321 tctgttaccg gaatgacatg cgccgcgtgc agcgctcatg tcgaaaaggc ggttgccgcg
     7381 attcccggag taaagagcgt gtccgtcagt cttcttacga attcgatgag cgttgagttt
     7441 gcatctcccg cttcggcgga atcgatatgt gctgctgtgg acaaggcagg atacggtgcg
     7501 gcgcccgaag atgtggactc aggtttgtcc gctgcggacg cggcaaggaa gcgggcttct
     7561 gaacgggatg cgctcgatga tcacgagacg ccgcgtctcg tgaaacggct tatcgcttcg
     7621 ctgattctgc ttgtgccgct cttgtatgtt tcgatgggac acctcatgtg gaagtggccg
     7681 cttcccgctg tattcagcga aaatccagct gcaatagggt tatacgaact gcttttgaca
     7741 ttagcaatca tgatgataaa ccagcgtttc tttactagcg gtttcaaaag ctttgttcat
     7801 ggcgcaccga atatggacac gctcgtcgcg ctgggcagcg gagcgtcggt cgtatatagt
     7861 gttgtcgttc tttttcttat aaccggtgcc atgctttccg gcatgttgca tgatgcgcat
     7921 gagtatctgc acgatctgta tttcgaatca gcagctatga ttctgacgct tataactgtc
     7981 gggaaaacac tcgaagcgta cagcaaagga aagacgacga acgcaatcaa aagtctcatt
     8041 gatcttgctc ccgacacggc gcacgtcatc cgcgacggaa aggaacttac gattcctgcg
     8101 gaacaggtgc ggaccggcga tacgtttatc gtgcgccccg gcgagcgtat ccccgtagac
     8161 ggaaaagtga ttagcggaac gagcgcggtg aacgagtcgg cgcttaccgg cgaaagtctt
     8221 cccgtcgaca agtcggccgg aagcgacgtg agcgcggcga cgctcaacca gaacggtttt
     8281 cttacgtgta cggcgacgaa agttggcagc gacacgacac tcagccagat aatcaccatg
     8341 gttgaaaacg ccgccgcgac aaaagcgcct gtcgcaagac ttgcagacaa agtgtcgggc
     8401 atattcgtcc ccatcgttat tgtactttcg gtgattaccg gaataatatg ggctgttgcc
     8461 ggaggagaat tcggcttcgt gcttgcgcgt gcagtcagcg tgctcgtaat aagctgtccg
     8521 tgtgcgctcg gtcttgcgac gccggtggca atcatggtcg gaagcgggct cggcgcaaaa
     8581 cacggcattc tgttcaagac ggccgccgcg cttgaagctg ccggaaagac tgatatagtc
     8641 gtgcttgata agacgggcac ggtcacggaa ggaaagccca cagtgacgga cgtaattccc
     8701 gcggacggag taactgaacg ggaactgctt gcgcttgcag gtgcgcttga ggaaaaaagc
     8761 gagcatcccc ttgcacgcgc agtgctggaa aaagtacggg cggaaaatat tgcatacagc
     8821 cctgcggagg attttaccgc gctgcctggg tacggcgtaa agggagtggt cccaccgtca
     8881 ggaaatgatt ccggagctgt ggctcctgtc gccggcgttc cggcatcagg cggaaatgcg
     8941 gcgcttatga aagaccagcg gctgctcggc aacgagatga aacagcgcgg tatggagctt
     9001 gccggcgaag gaaagacgcc gctttatttt gcagctggcg ggaaaatgct cggaattatt
     9061 gcggttgcgg atgtggcaaa aaaggacagc gccgacgcgg tgcagaaact caacgccctc
     9121 ggggtacaag tagtcatgct cacaggcgac aacagacgta cggcggaagc catcgcgaag
     9181 caggtaggca ttacacatgt cgtatcggat ctgctcccgg gcggcaagga cgccgtcgtc
     9241 gcgaagcttt cggcgttcgg gaaagtgatg atggtgggcg acggcataaa cgacgcacct
     9301 gcccttacgc gcgccgacac cggagttgcg atcggcgcgg gaactgacgt cgcactcgac
     9361 gctgcggatg tcgtgctcat gaaatccacg cttgtcgatg ttccggccgc aatcaaactg
     9421 tcgcgtcagg tacttacgaa cattcgggaa aacctgttct gggcgttcat ctataatatc
     9481 atcggcatac cggttgcagc gggagcgctt atacccgcat tcgggatcgt gcttaatccg
     9541 atgctcggag ccgccgcgat gagtctgtcg agcttctgcg tcgtgacgaa cgcgctccgc
     9601 ttgaacctgt tcaatatgta caagtcgggg cggaacggta agagaaaaac aatacagctg
     9661 ccggatgata ttttcgacgg ccggaatata tcacacaata ttcaaaactt gccagataac
     9721 tttgtaacac aatcgcctca tgtgtaaacc aaaccctatt tcacctttac ccagcgcaac
     9781 acctctttga gcttaggcct tttccctgtc atgagtatgc caactcggaa aacctttcct
     9841 gcaaatacca tcatgcccac aatcgacgca acaaggatgc caatacaaag tactagatgc
     9901 catactggag gcgatgcaac aaggattctg gatagcatca cgataggact cgtaagtgga
     9961 aaaaacgaca tgattacgac agccgttgaa tcggggctcg tcacgagcgt attgagcata
    10021 accatgggta ttatcaagaa catcaataat ggccatgcta tctgccccag atgctgctca
    10081 tcctcgctgg ctgctcccaa agctgcgtat gctgaggaat agagaaaaaa gcccaggata
    10141 aagaaaacga acagccacat catattttgc atagatactg ccacgggcaa agcgagtccg
    10201 aagagcggcc ccagaaagtg gttgaggaca aacgccatcg atatccaaac cgcatattga
    10261 agcataccgg cgagccccaa acccaatatt ttgcctatca tgagatctcg actcgtcacg
    10321 ctcgagagca taatctccac ggtcttcgag gttttttcgg ttacaacaga gcgccctatc
    10381 atctggccat agagcaatat agtcatatag agcaacatta taaatactag aactgtcata
    10441 agcgtctcga gaaaggcctg atctccctgg ccctgagcct tctcctcgcc tcccttttcg
    10501 agctttatta cctcgaaagt gggcttagag agcagctttg ctgccagagc tgggtctatt
    10561 ccgctttccc ttattcgctc ttcgcgcgct acagcctcta agaccgctga agccgtgcta
    10621 tataagccta tttctgcgct gctctttgag taccagatag cttttcctgt gtcaggccat
    10681 cccgcctcaa gttcaagcaa ggcaactcga gaaccatcga ttaccgaagc tttagctgct
    10741 tctatgtctt cgacgtgctc gacctggagc ttcatgccgg aaaatgcagc tgaaatagct
    10801 tgttcgaccc taggtggagc accgaagagg gcaagcggct tatccgatgc tactgggctc
    10861 gaagattgag cgagcaaagt gggcaggaca gttattgcaa agatgagaaa aggtccaatg
    10921 atggtgagga tgacaaaggc tttgtttgca gtggtgagcc tgaattcatg ccgaataata
    10981 tgttttagct tacccattct caatctcctc tcctcctacc agatggatga atatcttgtg
    11041 caaggatggg gctactgttt caaagcgccg aaccttgagc ctgcctgcca aagccttaaa
    11101 taaaaggtcg gagtcggtgc cttcctttag ttcgacttcg atccagcgcg gataggacac
    11161 cactgagtct acaaaatcaa ggctctgcac aaaagaagca tctccgtcaa attccagctg
    11221 aaccgcattg cgcccatatt gcgccttgat atcagccatc gatccatata ctatggcctt
    11281 gcctttattc atgagaagga ttttttcgca gatcttttca gcgtgctcca tgatgtgagt
    11341 agaaaagagt actatcgcgc ccttatgctt tagctcgacc attacggaaa ggagatcatc
    11401 ttgtgccata gggtcaaggc cggagaaggg ctcatcgaat aatacaaccg aaggattatg
    11461 agccactgtc gcgatgaatt gagctttttg cgccatgccc ttggatagat ccgaaatctt
    11521 gcgattcttc cattcggaga gcccgaatcg ctcgagccat gcatttatgg aaagacggta
    11581 ttcattcgcc tttgctcctt tgatggcagc gaggtattcc agaacttcgc ccaacttggc
    11641 tttgcgatac aggcctcgct cttcgggtaa atagcctatg tgatctttgt ctgcttcgga
    11701 caaaggtgct ccattgaagc gaatgatccc cgaatcaggc acgattatgt tcattatcat
    11761 acggatcgtt gtcgttttgc ccgctccgtt agggccaata agaccaaaga tctcaccggg
    11821 cacagcttcg aaggataaac cttccacagc cttaatgctt tcgtaggtct tgcatatttt
    11881 ctcgacggtt atcgtagctg acctccttac agtatcctat tcaattttct tcttcttacg
    11941 ttgacgcaca cattccgtta agagatactc aatctggcca tttatggagc gaaaatcatc
    12001 tgcagcccat gcagccagtt ctttccaaag ggtcggagag agtctaagga ggatctgttt
    12061 cttctcgtta tctttttcca atccttccat acttttacct cgacttagat tcccaaagtc
    12121 ctctcaaagc cgcgcctctt aaatcatgcc tcttgacctt aaccccttgg ttctaatctc
    12181 ctaactctaa cgcattaacc ctaatatata gaaccagtat tgactattgg ctgcggatcc
    12241 ttattagcgc acaatacgac aagcaaattg ctgaccatag tagcttttcg ttcctcgtcc
    12301 aagtttacga tattcctgtc gctcaattta gcaagagcca tctcgaccat gcctacggca
    12361 ccctctacga tcatctggcg agccgcaatg atagctgaag cttgttgacg ctgaagcata
    12421 gcggcagcaa tctcgggagc ataagaaaga tgagtgatcc gcgcttccaa tatttccaac
    12481 cctgccatgt ccactttctt ctggacctct tctttaaggc tctctgctat ctcctggcag
    12541 cttccgcgaa gggacttttc cccttcttct tcggaagcat cgtagggata gagcctgacg
    12601 atattgcgcg cagcggagtc gcactgaata gagagaaatt cgaaataatt gtccacattg
    12661 aatacggctt ttgcggtgtt gacgactctc catatcacta cgatactgat gatcacagga
    12721 ttgcctaagg catcgttgac cttctgcttc tcattgttga gagtcatggc cttgagtgat
    12781 atttttttgg aagcttgttt ggaagaggca ccatgggttt tgtgagcagc actgctagaa
    12841 cccgtctcac ctattggaag ggggatggat cccgatgaaa gctgtacatt agaaggacaa
    12901 accgatgcaa caaagggatt tacaaagaag aagccaggcc ctcttagcgt tccatgatag
    12961 ttgccaaaga gcgtcagaac catcgcttcg ttaggcttaa gaaccttgag gccagcaaag
    13021 agaataggcc cgatcaagaa tgcgtatagg ccacagacaa ccatgagcac gatcgtaccc
    13081 gcatccgttt ttcccgaaga tgttccaaaa acaaaaccag caacggtcac gatcataaga
    13141 aagacatcga gaataagcat cgccatgcca cttaagcttg gtgctgactt ttcttcattt
    13201 aggggacttt ggttcataat ggcctccctt tttcgaatta atgatatcat attgatatcg
    13261 atataatatc tcatttagtc ttggattttg tcaagggctt atatgcgctg gcggaaagaa
    13321 taggtgcttc gatgaaaatt ttcactccac atcggtgacc acatttctca ctccgacatc
    13381 tcaaaaatag tatttcgcac tcttgctaat ttcacctctg agattgcgca aaatgcacag
    13441 attttctgac gatgaaagcc tttttccagt aaatcgagat agcgtgctat agtgttcttg
    13501 ctagggccaa gcacttcagg atggagccca aagacaaagt atcattcgat ttctcgatat
    13561 atatcgatcc attcctgttt gagtttcagt tataagagcg aaatgtggaa aaggcatcgc
    13621 tgaatacttc gttcggcgat tagtgttctt caaaacctca tctcatcagt gccatcaata
    13681 gggcgccata tgcaattaca ataatgagaa aaattgcgag cggcaaagca catttcagaa
    13741 tcacctttat cagtgacgac ttaaagtgct gagcggtcac caccatgcag acatgaagtg
    13801 gcgaaagcat cattcctgtg taaccaaagg
//
LOCUS       DAOOWY010000048        13720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4493_length_13720_cov_10.907647, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000048 DAOOWY010000000
VERSION     DAOOWY010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4493_length_13720_cov_10.907647"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PK105_05130"
     CDS             <1..409
                     /locus_tag="PK105_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61126.1"
                     /translation="DPEWRTLSPMKEIGSITASELNDAQNYMDEYIRANDIPGIRMLV
                     VHQFADKMIQSREEVISHRDRVILIHTADGFGAPRLKKATYQRNADAENMPVKGFKLF
                     FKSDFALAGFDMPLMSPSEVMQLDPRPSLIIYQ"
     gene            487..>3925
                     /locus_tag="PK105_05135"
     CDS             487..>3925
                     /locus_tag="PK105_05135"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU61127.1"
                     /translation="MKKARVSAMMLILVSFSIVGVYAYDPPKGASLLGRIYGPWTMGG
                     GLSLTEKGDNTWNFLLNPASTSEEELPILALSYTGIADTSAGGPQGWGSAAMAAFSLP
                     KAYGVWNGSVRLFSAPSSMTSMPFGTFATIGGGFSKKISSGLSVGASAWLAMGGNGSF
                     GWGLWSDIGIVEEFGDMGFLKSPRLGFAFSGIGKEFNYETPPIGMSPGSTPSSGFPSA
                     FSPSLGFSADLLNDYNLRIMANIDLRAPSFADLEGEIGLGISYRNMLNLRISAAESLY
                     DIQKKTGRSLWPSITISGSIPLGSKDPQNKGVSAILPAFGFMPLYDSLNSFSIGAQIA
                     WRQHDMSPPEVKLELPPSVGGPPVAYISPNDDSKQDKLEIPLSVKDEQGIVAGWLFKI
                     EDRSSGKVVRTITEKSDLPDHIDSFSSIKKGFGYTKQNVPVPNILDWDGRDDEGNPVP
                     DGTYIVSFHAWDDLGKTNANYDSCMNVVVDRSAPIVNAWVLGSEQLLQGFDVMIFSPD
                     GDNSKDTISFRVQGSIEDNWKYEILNENNVAIRTMEIKARSAPRDFVWDGTDDRGKRV
                     PDGYYRFRLSTMDPAGNTGYKIIGISDDKSDWIVVDTSRPAVKVVADKKAFSPKKEGE
                     AGTAQISFELESLDNLVTWKEFIQDQNGKTVWTSSGDGSAPPPESFRFGGRDPSGEVL
                     PDGQYRAGIEMKYRNGYSPLSYSESFILDATPPFATIVLDDIRAIFSPDGDGRRDTLA
                     FSLSGSKEDTWKLSIFDEIGKPVYTREYREALPERFEWDGTDSTGRRVIDNKYTLEIE
                     SVDVAGNEFKVGFEPVVVDTRTPSAFFVLNREAFSPNDDGIADILGVKLKIDSIDGLL
                     DWKLYVSSALNGSVNAEKTGRQIALLQGDGTNSPPTDFQFDGKGEGALLKEGTYRFEL
                     FLEYENGWKTTKLSPEFVIDITAPRAQISRSREFFNPRGETKQSQVIISQIGSREALW
                     IGELRDNQGVLRCKWESKDSQPSEILWDGRTAEGALLPDGIYSYRLFCIDLAGNSFAS
                     EPILIGIDTVVKQSSLSADTMAISPNGDGIQDKMTLSISATAMESVQDWQLAILAGQE
                     RVMEWKGTGKVPQTIIWEGISLTRLPVPDGSYAAIFRAEYPNGDSTEASLGPLIVDRI
                     "
     assembly_gap    3926..4025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4026..7351
                     /locus_tag="PK105_05140"
     CDS             <4026..7351
                     /locus_tag="PK105_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU61128.1"
                     /translation="PQATVSLSRLIFSPNGDGINDTMRIEQDSVGKDRWQGSIFDLAG
                     KAVCTWSWEEAVRSFEWDGRDSAGNVAVDGKYRYQLVSKDAAGNGFSYISPIFEIETE
                     KKAVRLTVGDRAFSPNGDGVKDVQTIKAEVVSPEKVKEYVLQIVAQDGPAALSAVRTW
                     KGETPRSVYEWKGETDAQIQAPDGHYAASMKVVYRNGDEAEGATGTFLLDRQYPRIEV
                     HIQGSIFSPDGDGRSDTILITQKSLPGDDWKGAIKDSNGNIVQSWNWQNEAKDLVWDG
                     SNMSGSTVSDGYYVYSVESEDIAGNKTVVGPFQIQVETGRRTVQLRLSDKAISPNGDG
                     IKDELVINVDVDARDRIKQFNLAIRSKDGAVMKAWTGGADLSREYRWTGTNEAGAAVP
                     DGDYTVSLEVLYLNDAFAKAGPMAVLVDRIAPQATVSLSRLIFSPNGDGRADTVELIQ
                     SSVYGDRWNGQIISETGKIVRTWEWYPMLADVLWDGKDQSGKLVSDGVYYYELRSIDA
                     AGNFFILPRQQIIVDAAQKNVSFKVEPTAFSPNDDGVKDLIYLNVNAPKPDTLLSYSL
                     SIFAGSKAAAGQAPVRSWKGKIDIKSQYVWDGMTDAGLKVPDGYYSTYLELEYANGDL
                     FNFGPIQILVDTVPPRISISADPLLFSPNGDGIKDTITIAQESVPGDDWSGRIRSASG
                     ATVRSYTWKGQAKNFTWDGKDSNGKLVPNGAYSYEVVSVDAAGNSASASIKGIMVDAT
                     KPRVFVTASDNGISPNSDGIRDEVSFSLTVENREGVDSWRFSLLDSKGVERSFFGGSG
                     SDVPTRLVWDGRDLNGQVIEDTFTGKLIVRYMKGDIAEATSSKIVVKIAPPQVEIKVK
                     PEYFSPDDDGVDDMLTFGIVADKNAGITEWKLEVLETAIVESAALQESKPTRSFMLWS
                     GKGMPPSQITWNGKSPKGELVESATDYPFEFTCWDALGNQTKISGIIAVDVLVIRDGD
                     RLKIRVPSIVFRANHADFIGLDSEIVARNEKVVTRIAQILGKFPDYRIRVEGHGNNVG
                     KMLGYSASRIQQEEINELIPLSTERAEVVRKMLVENGVDARRLTVVGLGSSEPVVPFT
                     DVQNRWKNRRVEFVLIKNQ"
     gene            complement(7503..8450)
                     /gene="murB"
                     /locus_tag="PK105_05145"
     CDS             complement(7503..8450)
                     /gene="murB"
                     /locus_tag="PK105_05145"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOU61129.1"
                     /translation="MKNLWQIAQKINTDHCLILFDEPMKEHTTFKIGGPADLFIRPRS
                     ISALEEVVELLKIENVPFFILGSGANILVGDRGVRGAVIDTSLLSSIMHLKREERIDP
                     VLLYAECGVRLMTLCEEAQICGLSGLENFYGMPGSLGGSVYMNARCYEDDIATHIQDL
                     TIIDNQGRLKIISACAQSWSYKRSPFMRGGELESLIICAASFRLIPDDPEKIAERMRL
                     CMRDRMQKRHFNFPSAGSMFKNNHDFGKPTGKILDELGLRNYRIGDAAISPWHANIFV
                     NIGKASARDMRKLIEYAQKVVYDATGFCIEPEVLFIGEF"
     gene            8517..9944
                     /gene="cysS"
                     /locus_tag="PK105_05150"
     CDS             8517..9944
                     /gene="cysS"
                     /locus_tag="PK105_05150"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOU61130.1"
                     /translation="MHDVHIFNTMGRKIQLFTPLHEGNVGFYGCGPTVYNYAHIGNLR
                     TYIFEDTLVRMLRRFGYRVTHVMNITDVGHLSGDDDTGEDKMLKSAREKGQSVLEIAQ
                     FYTNAFFKDTERLNIRQPDIVCKATDHIQDMIELIKRIEANGYSYFSGGNLYFDVSKF
                     DHYGELAGLQLDQLKAGARVELDPNKRNPFDFVLWFTKSKFDKQALIWDSPWGIGYPG
                     WHIECSAMSMKYLGEQFDIHAGGVDHIAVHHTNEIAQSEAATGHRWVNYWMHAEFLVL
                     DKGKMSKSSGSFLTLESLIDKGYDPLDYRYFCLGGHYRSQLSFSWEGLDQARSARLSL
                     LDRVLALKEKAVKPASISELGQKALRYLTAFDDALADDLAMPRALAQVWSLLKDNDVP
                     AQDALALILNIDEVLGLDIAKAQRREVSVDTDTVQWINSQIQRRAAAKKARDFATADA
                     IRSELVARGIQLEDRPEGTVWRLIE"
     gene            9981..10577
                     /locus_tag="PK105_05155"
     CDS             9981..10577
                     /locus_tag="PK105_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU61131.1"
                     /translation="MFQNKDDGPISDYNLIFAQNVDLLLRIAVHITNDWESAEDIVQD
                     AFGKLIEKQMQFPSEEDARFWLIRVVKNGAINWSRRKVREFKAYEHWWNAETTQAKIS
                     ADALDSRDMQNQHAGTDQSADHELMMKECAQEVRDALMRIPEKLRMVLILKEYEGMNY
                     KEIAKVLGITEGNVKVRAFRAREALLSILNEGGSYVSR"
     gene            10564..11184
                     /locus_tag="PK105_05160"
     CDS             10564..11184
                     /locus_tag="PK105_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61132.1"
                     /translation="MCPDESLLTAYIDNEVPSPWKERIEMHLCQCERCANRVAQYRAL
                     KVALKAADVLEASKRQEAAKRIQASLKSITDESSRPLRTEGIIERYPVFSTFMSKRIS
                     VPLPILAASLLIMVFFAGLAFGIFGAHRTTGQALALSTRLPEESASNIESIVSLLSQT
                     DPNQFVTIHAPGNISQPLVNTTPVYVIYNSSGRKPTVMAFPVEGER"
     gene            11186..11749
                     /locus_tag="PK105_05165"
     CDS             11186..11749
                     /locus_tag="PK105_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61133.1"
                     /translation="MKRSTLTLAAISILNVAWLFAQTPSIPTAPSSIAIPDSPSSNIP
                     VFQFQIRTAFTLTPGMVSAIQSASLGSQGSKAAQGESIEKANSPKWEDAIVREVSLAS
                     PLGIKIQNDRFIAIIVLMPIELIKKDITMLVQNHIYMRSQDNSIQLNTSVHTVRIPVD
                     SVFYYYPLGGDSKQGAPMVIEILVNSK"
     gene            11754..13388
                     /locus_tag="PK105_05170"
     CDS             11754..13388
                     /locus_tag="PK105_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU61134.1"
                     /translation="MKQRRIITLCILIFLVAAGIIIYRNMQPRLLAIDPVIVMSGDLV
                     NIAGRNLGKKEGELLLDNVPLPSQAILFWSPFLINFRMPSNYDSAVVRVKTGLGVSNP
                     LMVALASKVPSKAENNLIQQHNPSITSIKPSTNAQIGKLIELRGSHFGMPEEESAVLF
                     SKVPIISTLEAIDADNFARVESISLLVDKWNDSYIAAYVPDGTENGYVLVRTRNGLSN
                     AFPISISRNPGRLSRGDSVTYTLKDTLVVHIAASLPDGRVSFILSKPPVTQYQSTMAT
                     IEAGADHLRAETAVWQEFRLGAADARLGRIELQRRLIIDVCEITADITIGTLQPLGTK
                     PPAFLSPYLAEDALVPSTNPSIISAALSMKKKEKNPFRQIMLATQWTFSNIKLKQEKQ
                     SFGDDPIDALKSKAGGIRSLVLINCALLRALNIPAIPVAGFLVTEDMRLIPHYWSEYY
                     LIGIGWIPFDPALAIGMIPSGFRPVFSNRSNYYQGIDNKHIAMARGYQLLPSIPAEAQ
                     RKSKIPWSLFEYDEVAHGLSYTSTWNPPILLSTTIQ"
     gene            13474..>13720
                     /locus_tag="PK105_05175"
     CDS             13474..>13720
                     /locus_tag="PK105_05175"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307286.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOU61135.1"
                     /translation="MSYIKSMDPELYAAMEAEASRQREKIELIASENYTSKAVMEAVG
                     SVLTNKYAEGYPGKRYYGGCEFVDIAENLARDRAKKLF"
BASE COUNT         3778 a         2849 c         3429 g         3564 t
ORIGIN      
        1 agatccggag tggagaacgc tcagccccat gaaagaaata ggatctatta ccgcctctga
       61 gctcaatgat gctcaaaatt atatggatga gtatatcagg gcgaatgata ttcctggcat
      121 acgtatgctt gttgtgcatc agttcgcaga taagatgatt caaagccgcg aagaggtgat
      181 atcgcacagg gatcgtgtca tccttattca taccgctgat ggtttcggtg ctccaaggct
      241 gaagaaagcc acctatcaga gaaacgcgga cgcggaaaat atgccggtaa agggttttaa
      301 gctctttttt aaatcggatt ttgctctggc gggattcgat atgccactta tgtcgcctag
      361 cgaagtcatg cagcttgatc caagaccaag cctgatcata tatcagtgat gagacagccc
      421 tgaagttggg ctcttgctgt gatagattga atttgaaggt ttctttttat agagaagggg
      481 atatagatga aaaaagcccg ggtatctgca atgatgctta tattggtctc ttttagtata
      541 gtcggagtct atgcctatga tccgcctaag ggagcatcgc tgcttggccg tatttacggt
      601 ccttggacaa tgggcggggg attatcgctc accgaaaaag gggacaacac atggaatttc
      661 ctattaaacc cggcttctac ttctgaagaa gaacttccta ttctcgcttt atcttacaca
      721 ggcatcgctg atacctccgc tggtgggcct caaggttggg gcagcgcagc aatggcagct
      781 tttagcctgc caaaggccta tggagtatgg aacggatctg ttcgcctttt tagcgctcca
      841 agttcaatga caagcatgcc atttgggacc tttgccacta ttggaggtgg attttccaaa
      901 aaaatctcaa gcggtctatc cgtgggagct tcggcttggc ttgcaatggg aggaaatggc
      961 agctttggat gggggctttg gtccgatatt ggaattgtag aagaattcgg cgatatggga
     1021 tttctaaaaa gtccaagatt aggttttgcg tttagcggaa ttggaaagga gttcaactat
     1081 gagacaccgc caataggcat gtcgccagga agcacgccat cttctggttt tccttctgct
     1141 ttcagcccaa gtcttggatt cagtgcagat cttctcaacg attacaattt gcgcataatg
     1201 gccaatattg atttgcgcgc gccgtcattt gcggatctag agggtgagat aggattaggt
     1261 atttcttatc ggaacatgct taatcttcga atctctgcag cagaaagcct ctatgatatt
     1321 caaaagaaaa ctggacgaag tttatggcct tctattacga tatcgggcag catacctctt
     1381 ggcagcaaag atcctcagaa caagggagtt tcagcgatac ttccggcctt tggattcatg
     1441 cctctttacg atagcttgaa ttcattctct attggtgctc agattgcttg gagacaacat
     1501 gatatgagcc ctcccgaagt gaagctcgaa cttccgcctt cggtaggcgg tccgcccgta
     1561 gcttatattt cgccaaatga cgatagcaaa caggacaagc tagagattcc tctttcggta
     1621 aaggatgaac agggaattgt agccgggtgg ctcttcaaga ttgaggaccg ctcttcaggt
     1681 aaggtggtac ggacaattac tgaaaagtct gatcttcctg atcatatcga ttctttttct
     1741 tctataaaaa agggatttgg ttatacaaag cagaatgtgc cggttccgaa tatcctggat
     1801 tgggacggca gggatgatga aggcaatcca gtaccagatg gaacttatat agtgagtttt
     1861 catgcttggg atgatcttgg aaaaacaaat gcaaattatg actcttgcat gaatgtcgtt
     1921 gtggaccgaa gtgcaccaat agtaaatgca tgggttttgg gcagcgaaca gcttttgcaa
     1981 ggttttgacg taatgatttt tagccctgat ggtgacaata gcaaggatac aatctccttc
     2041 cgtgttcagg gctctataga agataactgg aagtacgaaa ttctcaatga aaacaatgtc
     2101 gccattagga caatggaaat caaagccaga agtgctccgc gcgactttgt ctgggatggt
     2161 actgatgata gagggaagag agtccccgat ggttactatc gtttcaggct ctctactatg
     2221 gatcctgctg gaaacactgg ttacaaaatc atcggcatat ctgatgataa gtccgactgg
     2281 atcgtagtag atacaagcag acccgcagtt aaggttgtag ctgacaagaa agcctttagc
     2341 ccaaagaaag aaggagaggc gggtactgca caaatttctt ttgaacttga gtctttagat
     2401 aacctggtta cctggaaaga gttcattcag gatcaaaatg gcaagacagt ctggacctcc
     2461 tccggcgatg gctcagcacc tcctcctgag tctttccgat ttggtggacg tgatccttct
     2521 ggagaagtgc ttccggatgg acagtataga gcaggcattg agatgaaata tcgaaatgga
     2581 tattctcctc tcagctactc ggagtctttc atactcgatg ctacgccgcc attcgccaca
     2641 attgttcttg atgatattag agctattttc agccccgatg gagatggaag aagagataca
     2701 ctggctttta gcttatcagg aagcaaagaa gatacatgga aacttagcat cttcgatgaa
     2761 attggtaagc ctgtctacac acgagagtat cgcgaagcac ttccagagcg ctttgaatgg
     2821 gatgggaccg attcgacagg ccgtcgcgta attgataata agtatacgct agaaatcgaa
     2881 agtgtagacg ttgcgggcaa tgaatttaag gttggttttg aaccggttgt tgtagataca
     2941 agaacgccaa gcgctttttt tgtgctcaat agggaagcct ttagcccgaa tgatgatggt
     3001 atagcagata ttcttggtgt caaattgaaa atcgacagca tcgatgggct tctagactgg
     3061 aagctctatg tttcatctgc cttgaatggt tcagtgaatg ctgaaaagac aggcagacaa
     3121 atagcccttc tccagggtga tggaaccaat tctccaccca ctgatttcca attcgatggt
     3181 aagggagagg gagcattgct aaaagaaggc acttatcgct ttgaactctt cctggaatac
     3241 gaaaatggct ggaagacgac aaaattgagt ccagaatttg taatcgatat cactgcacca
     3301 agagcccaga tttcgcgttc gagagagttc tttaatccgc gtggcgagac taagcagtca
     3361 caggtaatca tctctcagat tggctcgaga gaagcactat ggataggaga gttgcgggac
     3421 aatcagggtg ttctgaggtg caagtgggaa tctaaggaca gtcagccatc tgaaattctc
     3481 tgggatggta gaactgccga aggagcgctc ctccctgatg gaatctatag ctatcgccta
     3541 ttctgtatag atctggccgg caacagcttt gcctcggagc ctatcttgat tggcatcgat
     3601 actgttgtca aacagtcctc gctgtctgct gatactatgg caattagccc aaatggagat
     3661 gggattcagg acaaaatgac cctttccata agcgcaacgg cgatggagag cgtccaggat
     3721 tggcaacttg caatacttgc cggacaagag agggtcatgg agtggaaagg taccggaaag
     3781 gtgccacaga ctattatctg ggaagggata agtctgactc gtttgcctgt tcctgatggc
     3841 agctatgcgg ctattttccg agccgaatac cccaatggag actccaccga ggctagtttg
     3901 ggccccctca ttgttgatcg tatagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnncgcct caggcgacag taagtctttc tcgtttgata ttttctccga atggagatgg
     4081 gataaatgac acgatgcgca tagagcaaga ttcggtaggg aaagacagat ggcaagggag
     4141 tatttttgat ttagcgggca aggctgtgtg tacgtggagt tgggaagaag cggttcggtc
     4201 ttttgaatgg gatggtagag acagtgctgg caatgtggct gttgatggga aatatcgata
     4261 tcagcttgta tcgaaagatg cggcgggcaa tgggtttagc tatatcagtc caatttttga
     4321 aatagagact gagaaaaagg ccgtgcgtct tacggttggg gatcgtgctt ttagtcccaa
     4381 tggtgatggt gtgaaagatg tgcagacaat aaaagcagaa gttgtctcgc ctgaaaaggt
     4441 aaaagagtac gtgctacaga ttgtcgcaca ggatgggccg gcggcattga gtgcagtaag
     4501 gacatggaag ggtgaaacgc cacggagtgt ctatgagtgg aaaggtgaaa cggatgctca
     4561 aatacaagcg cccgatgggc attatgctgc atcgatgaag gttgtgtatc ggaacggtga
     4621 tgaggccgag ggtgccactg ggactttttt gctagacagg caatatccac gaatagaagt
     4681 gcatattcag ggtagtatat tttctccgga tggggatggc agaagcgaca ctattcttat
     4741 aactcaaaaa tcattgccag gcgacgactg gaaaggggcg ataaaggata gcaatggcaa
     4801 tatagtgcaa agctggaact ggcaaaatga agccaaggat ctggtatggg atgggagtaa
     4861 tatgagtgga tctacggttt ccgacggcta ctatgtttat tcagttgaat cggaggacat
     4921 tgccggcaac aagaccgtgg ttgggccatt tcagattcag gttgagactg gaagacgtac
     4981 tgtacagctt cgtctcagtg ataaggcgat aagtcccaat ggtgatggaa taaaagacga
     5041 gcttgttata aacgtcgatg tggatgcgcg ggatcggata aaacaattca atctcgctat
     5101 tcgttccaaa gacggtgcgg ttatgaaggc atggactgga ggtgccgatc tttcccgaga
     5161 gtatcgctgg actgggacca atgaggcagg ggctgcggta ccggatggag actacactgt
     5221 gagtcttgag gttttgtact tgaatgatgc atttgccaag gctgggccta tggctgtact
     5281 agttgatcgt atagcgcctc aggcgacagt aagtctttct cgtttgatat tttctccgaa
     5341 tggagatgga cgagccgata ctgttgaatt gatacagagt agcgtatatg gggatcgatg
     5401 gaatgggcaa ataatctcag agacaggaaa gattgttcgc acctgggagt ggtatccgat
     5461 gctcgccgat gttttatggg atggcaaaga ccagagtggt aagcttgtat ccgatggtgt
     5521 ttattattat gagctgagat cgatcgatgc ggccggcaat ttcttcatcc ttcctcgtca
     5581 gcagatcata gtcgatgcag cacagaaaaa tgtcagtttc aaagttgaac ccacggcctt
     5641 cagcccaaat gacgatggcg tcaaggatct aatttactta aatgtcaatg cgccaaaacc
     5701 ggataccctg ctttcttact cactttccat atttgctggt tctaaagccg cggcaggtca
     5761 agcgcccgta cggagctgga aaggaaaaat cgatattaag tcgcaatatg tctgggatgg
     5821 tatgacggat gcgggattga aagtgccaga tgggtattat agtacgtatc ttgagcttga
     5881 gtatgcaaac ggcgatctat tcaatttcgg ccctattcag atacttgtgg atacagttcc
     5941 gccaaggata agcatatccg cggatccctt gcttttctct ccgaatggcg atggcatcaa
     6001 ggataccata accattgcgc aggaatcagt gccaggtgat gattggtcag gcagaattcg
     6061 gagtgcatcc ggggcaacag tacgaagcta tacctggaaa ggacaagcaa agaactttac
     6121 ctgggatggc aaagactcga acgggaaact tgtgccaaat ggcgcttatt cctacgaagt
     6181 agtcagtgta gatgctgctg gtaatagtgc ttcagcttca atcaagggta ttatggtaga
     6241 tgctacaaag ccgagagtct tcgtaactgc ttcagacaat ggaatttcgc ctaatagcga
     6301 tggaattcga gatgaagtat ccttctcgct tacggtagaa aatcgagagg gtgtcgattc
     6361 gtggcgattc tcccttcttg attctaaagg tgttgaacgc tccttctttg gtggaagtgg
     6421 ttcagatgtg cctacgcggc ttgtatggga tggccgagac ttgaatggcc aggtgataga
     6481 ggatactttc acaggcaagc ttatcgttag atacatgaag ggtgacatag cagaggcgac
     6541 aagcagcaaa atagtggtca agattgcacc tccgcaggta gagattaagg tcaagccaga
     6601 atatttcagc cctgatgacg atggtgtgga tgatatgcta accttcggta tagtagcgga
     6661 caagaatgcg ggcatcacag agtggaagct cgaagtgctc gaaaccgcca ttgtcgaatc
     6721 tgccgccttg caggaaagca agccaacgcg atctttcatg ctctggagcg gcaagggaat
     6781 gcctccttct cagatcacct ggaacggaaa atcccccaag ggagaattag tcgaatctgc
     6841 aaccgattat ccattcgaat tcacctgctg ggacgccctc ggcaaccaga ctaagataag
     6901 cggcataatt gcggtggatg ttcttgtcat acgcgatgga gacaggctca agattagagt
     6961 gccctctata gtctttaggg cgaaccatgc ggacttcatt ggcctggata gtgagattgt
     7021 ggctcgaaac gaaaaagtag taactcgaat tgctcagatc ctaggcaaat tcccggatta
     7081 taggataagg gtcgaaggtc atggaaacaa tgtcggcaag atgcttggat acagcgcatc
     7141 gcgtattcag caagaagaga ttaatgagct gataccatta tctaccgagc gagcagaggt
     7201 agttcgtaag atgctggtgg aaaatggagt agatgcgcgt cgcctgactg tggtaggact
     7261 tggttcgagc gaaccagttg ttcccttcac cgatgttcaa aaccgttgga agaatagacg
     7321 tgtggagttt gtgctgatca aaaatcagtg agcttttatt gctaatcgat ataattgagg
     7381 ggctgctcat ttagttgggc agcccttttt ttctgcttat atatgccact ttcgcgctag
     7441 gaaaccttgc tgcattttag cgagaacatt ttagcgagaa taagaatata ccaaagtgct
     7501 aatcagaact ctcctatgaa taggacttct ggctcaatgc aaaaccctgt agcgtcataa
     7561 acaaccttct gagcatattc tattagtttt ctcatgtcgc gagcgctggc ttttcctata
     7621 ttgacaaata tgtttgcatg ccacggagag atagctgcat cgccgattct atagtttcgc
     7681 aagccaagtt catccagtat ttttccggtt ggcttgccga aatcatgatt atttttgaac
     7741 atagatcctg cgcttggaaa gttaaaatgc cgcttttgca tgcgatctcg catacataag
     7801 cgcatacgct cggcgatttt ttcgggatca tctggaatga gcctaaacga tgctgcacag
     7861 attattagag attctaattc gccgccgcgc atgaagggtg agcgcttata ggaccaggac
     7921 tgagcgcagg cggaaatgat tttgagtctt ccttggttat cgataatggt aagatcttga
     7981 atatgcgtgg ctatatcatc ttcataacag cgcgcattca tatagaccga acctccaagg
     8041 cttccaggca ttccatagaa attctcgagc ccagaaagac cacaaatctg agcctcttcg
     8101 cacagagtca tcaaacgaac tccacactcg gcatatagga gcacagggtc gattctctct
     8161 tcccgtttca aatgcatgat cgaagagagc agactggtat cgatcaccgc tcccctaaca
     8221 cccctgtctc ccacaagtat attagctccg cttccaagta tgaaaaaagg tacattctca
     8281 atctttagca gctccactac ctcttcaaga gccgatatgg agcgcggtct tataaagaga
     8341 tcggcaggac caccgatctt aaaagttgta tgctccttca taggctcgtc aaaaagaatc
     8401 aagcaatgat cagtattgat tttttgagcg atttgccata ggtttttcat gtaattccag
     8461 tatcttagct taaagaggca aaagcaagaa ggtccgactg acgaaaagga gcacatatgc
     8521 atgatgtcca catttttaat acgatgggtc ggaaaattca gctttttacg cccctgcacg
     8581 aggggaatgt gggattctat ggctgcggac ctaccgtcta caattatgct cacataggaa
     8641 atctcagaac atatatcttc gaagatactc ttgtccggat gctaaggcga tttggatatc
     8701 gggtgacgca tgtaatgaat attactgacg tgggtcatct ctccggtgat gatgatacag
     8761 gcgaagacaa aatgcttaaa tcagccagag aaaaaggcca atcagtgctt gaaattgccc
     8821 aattctatac caacgctttt tttaaggata ctgagcgctt gaatattcgg caacccgata
     8881 tcgtctgcaa agcaacagac catattcagg atatgatcga actcatcaag cgcattgagg
     8941 caaatggtta cagctatttc tccggaggga atctctattt cgatgtctcc aaattcgacc
     9001 attatggcga acttgccggg cttcagctag atcagctcaa agcaggcgca cgcgtcgagc
     9061 tggatcctaa taaacgcaat cctttcgact ttgtgctttg gtttacaaaa tcgaaattcg
     9121 ataagcaagc gttaatctgg gatagcccat ggggaatagg atatccgggc tggcatatcg
     9181 aatgctcggc catgagcatg aaatatctgg gagagcaatt cgacatacac gcgggagggg
     9241 tcgatcatat agccgtgcat catacaaatg aaattgctca atccgaagcc gcaacaggcc
     9301 acagatgggt caactattgg atgcatgcgg aattcctcgt tcttgataaa ggcaagatgt
     9361 ccaaatcttc gggcagcttt cttacgcttg agtctctcat cgataaaggc tacgaccctc
     9421 tggattatcg gtatttctgt ctgggtgggc attatcgaag ccagctctca ttttcctggg
     9481 aaggactgga tcaagcgaga tcagcaaggc tctcgctact ggatagggtt cttgcactca
     9541 aagaaaaggc agtgaagcca gcatccatat cggagctagg tcaaaaagca ctccgatatc
     9601 tcactgcctt cgacgatgcc ttggccgatg acctagcaat gccgcgtgcg ctagctcagg
     9661 tatggagctt attgaaggac aatgatgttc cggctcaaga tgctcttgcg ctcattttga
     9721 atatagatga agttctgggt ctggatattg ccaaggctca gagaagagaa gtctcggttg
     9781 atacagatac cgttcaatgg atcaatagtc agatacagag aagagcggca gccaaaaagg
     9841 cacgagattt tgcgacagcc gatgcaattc gcagcgaact tgtggcaaga ggcattcagc
     9901 ttgaagatag acctgaggga acagtatgga gattgatcga atgattatga aaggctttct
     9961 gtaacaaaag gattgatgga ttgttccaaa ataaggatga tgggccaata tcagactaca
    10021 acctcatatt tgcgcagaat gtggacctac ttctgcgcat agccgtgcat atcaccaatg
    10081 attgggaatc tgcagaagac atagtccagg atgcctttgg taagttgatc gaaaagcaaa
    10141 tgcaattccc ttcggaagag gatgctcgat tctggctcat tcgagtggta aaaaatgggg
    10201 ctataaactg gtcaaggcga aaagtgagag agttcaaggc ctacgagcac tggtggaacg
    10261 ccgaaacaac tcaggcaaag atttctgcag atgctcttga ttcgagggat atgcaaaatc
    10321 aacatgcagg aacagatcaa tccgcagatc atgaacttat gatgaaggaa tgcgctcaag
    10381 aagtgcgaga tgctctgatg aggattcccg aaaagctgcg aatggtactc attctcaaag
    10441 aatatgaagg catgaattat aaagagattg ccaaggttct aggcattacc gaaggaaatg
    10501 tcaaagttag ggctttccga gctagagaag ctcttctttc gatattgaat gagggaggat
    10561 cttatgtgtc ccgatgaaag tctgttgacg gcttatatcg ataatgaagt accatcacca
    10621 tggaaagagc ggatcgagat gcatttgtgc caatgtgagc gatgtgcaaa tcgtgttgct
    10681 caatatcgcg ctctaaaggt agcattaaag gccgccgatg ttttggaagc ctcaaaacgg
    10741 caagaagctg ccaaacggat acaggcttcg ttgaagagca ttacagatga gtcttcgcgc
    10801 ccacttcgca cagaaggtat aatcgagaga tatccagtat tttctacgtt catgtccaag
    10861 cgaatatctg tgccgcttcc tattctagct gcatcgcttc tcattatggt attctttgcg
    10921 ggcttggctt ttggtatttt tggagcccat agaaccacag gccaagcatt ggctctgagc
    10981 acaaggctcc ccgaagagtc cgcatcgaac atagaaagta tcgtgtcctt gctttcccag
    11041 acagatccga accagtttgt gacaatacat gcgccgggta atatatctca acctctggtc
    11101 aatacgacac ctgtgtatgt gatctacaat tcctccggcc gcaagccaac agtcatggcc
    11161 tttccggtgg aaggggagag ataagatgaa gcgttcgact ctcacccttg ctgcaatttc
    11221 gattctgaat gttgcatggc tttttgctca gacgccctca ataccaacag caccttcttc
    11281 gattgcaata cccgattcac catcttcaaa tattcccgtt ttccaatttc agattagaac
    11341 tgctttcacc ttgacacctg ggatggtatc tgcaatccag tcagcttcgc ttggatctca
    11401 agggtcaaaa gcagctcaag gagaatccat cgagaaagca aattctccca agtgggaaga
    11461 tgcgatagtt cgtgaagtaa gcctcgcttc tccgcttggt atcaaaattc agaacgatag
    11521 attcatcgca ataatagtat tgatgcctat cgaactcata aaaaaggaca tcaccatgct
    11581 tgtccagaat cacatctata tgcgaagcca agataattcg attcaattga atacctcggt
    11641 tcatacggtg agaataccgg ttgattcggt attttattac tatcctttgg gtggagattc
    11701 caaacagggt gcacccatgg tgatcgagat tcttgtcaat tcaaagtaag tccatgaaac
    11761 agcgtaggat tattacgctt tgtattttga ttttcttagt cgctgcagga ataatcatct
    11821 atcgaaacat gcagccgagg cttttggcaa ttgatcctgt aattgtcatg tccggcgatc
    11881 ttgtcaatat tgcgggacgc aatcttggca aaaaggaagg cgagctcctt ttggataatg
    11941 ttcccctgcc ttcacaagct atactcttct ggagcccttt tcttataaat ttccggatgc
    12001 ctagcaatta tgattcggcc gttgtgcggg taaagacggg gctgggggta tcgaatccac
    12061 ttatggtggc gcttgcttcg aaagtccctt caaaggcaga aaataatcta attcagcagc
    12121 acaatccaag cattacaagc ataaaacctt cgacaaacgc tcagatagga aaacttatcg
    12181 agctcagagg cagtcatttc gggatgcctg aggaagaatc tgcggtgctg ttttcgaaag
    12241 tgcctataat ttcaacgctt gaagcgatag atgcggacaa ttttgcaaga gtcgaatcga
    12301 ttagtcttct tgtagataaa tggaatgact cttatatagc ggcttatgtg ccggatggta
    12361 ccgaaaatgg gtatgtctta gtccgaacaa gaaatggtct ctcgaacgcc tttcctattt
    12421 ctatttcgcg taatcctggc agattgtcca gaggagattc cgtaacatat acgctcaaag
    12481 atacgctcgt ggtccatatt gccgcatctc tgccagatgg cagggtatcc ttcatcctct
    12541 ccaaaccgcc tgtaacgcag taccagtcga caatggcgac gatcgaggca ggagccgatc
    12601 atctgagagc cgaaacggcg gtatggcagg aattcagact gggtgcggcg gatgcaagac
    12661 tggggagaat agaattacag aggcgattga ttatagatgt atgcgagatc acagcagata
    12721 ttaccatagg cacgctacag ccactcggaa caaagccccc tgcttttctt agcccttatc
    12781 ttgcagagga tgctctggtt ccttctacta atccttcgat aatctcggcc gccctgtcga
    12841 tgaagaaaaa ggaaaaaaat ccttttcgac aaattatgct ggctacccag tggacattct
    12901 cgaacataaa gctaaagcaa gaaaaacaga gctttggaga tgatcctatc gatgcactca
    12961 aaagcaaagc cggtggtata cgttctcttg ttttaatcaa ttgtgctctt cttagagcat
    13021 tgaatattcc agcaatcccg gtagcgggct ttcttgtgac cgaagatatg cgcttaattc
    13081 ctcattattg gtcagaatat tatcttatcg gaataggatg gataccattc gacccagccc
    13141 ttgcaatagg aatgattcct tcgggttttc gtccggtttt ttcgaataga tccaactatt
    13201 accaaggaat tgataacaag catattgcaa tggctagggg gtatcaactt ctaccttcta
    13261 ttccagcaga ggcacagaga aaaagcaaaa tcccctggtc acttttcgaa tatgatgaag
    13321 tagctcatgg attaagttat acttcgacct ggaatccgcc gattcttctc tcgaccacga
    13381 ttcagtaatt cttacaggaa gatgggcaca cgttagtcgg ttttttgtag tatactaata
    13441 aatgccatga tacaataatt cgcgggagga gatatgtcct acattaaatc gatggacccg
    13501 gaactatatg ctgcgatgga agccgaggca agccgtcagc gtgaaaaaat cgagctaata
    13561 gcaagcgaaa actatacctc caaagctgtt atggaggctg tcggttcggt ccttacaaac
    13621 aaatacgccg aaggataccc tggaaagcgc tactacggtg gctgcgaatt tgtcgatatt
    13681 gctgagaatc tagcccgtga cagggcgaag aagctttttg
//
LOCUS       DAOOWY010000049        13545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4572_length_13545_cov_5.344181, whole genome shotgun sequence.
ACCESSION   DAOOWY010000049 DAOOWY010000000
VERSION     DAOOWY010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 13545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13545
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4572_length_13545_cov_5.344181"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..1654)
                     /locus_tag="PK105_05180"
     CDS             complement(347..1654)
                     /locus_tag="PK105_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOU61136.1"
                     /translation="MNSQDEYNRKLISIPEAVSKIKSNDNIVVAMCASEPQGSMMQFH
                     TVADKVENVRVFSCLTLKPYDFYMKPEMKGHFELASWFHAPGPRAALKANVGTVTYVP
                     NMLHRAAADFIYARKPNIFWGTCTPPDKHGFVSLSLGITYEKDILEASDLVILEVNPR
                     LPRTFGDTHLHTSEVDYFVECDQPVPEQPSPQPNELDLTIGNYIGDLIENGSTIQLGI
                     GGIPNAAALALEHKKDLGVHTEMIVDSMMELYEMGVITNSEKAFHKGKFVATFSMGSR
                     KLYDWLDDNVAVEFMRGSWVNHPSILAQNSKMVSINTCLTVDLTGQVGSESLGPVQYS
                     GTGGQTDTAEGAAEGFDGKGKSIIACYSTAKHDSISTIVPTLMEGTAVTLHRSLTDYV
                     VTEYGIASLRGKTLRERARELISVAHPKFREELTWKAKELGWL"
     gene            complement(1750..2757)
                     /locus_tag="PK105_05185"
     CDS             complement(1750..2757)
                     /locus_tag="PK105_05185"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOU61137.1"
                     /translation="MAKAVIVGTGIYVPGAPIDNKTVMKLTGIEFDAARHEQKIGIKQ
                     RHISRLSGLQETTADFAEKAARDALKAAKIDAEDIGLFIVATDTPEHISPATSIIVQG
                     RLQGGQTYAKSFDLGASCASFVEALDVAARNLMTDTSVHYALVIGVYNMPAFIRDDDS
                     FGWSIFADGAGAVVLERQEESNSGYIDGTFITDGTQWNFLGVYAGGTRFPITHERLNK
                     GEYGLQLLQKMPGDRNIKLWPPLIQHLLDKVHLPQSEVDHYLFTQINKSVIEQVMEIL
                     GEPIEKTTMVMDRYGYTGSGCIPMAMHVGLQEGRIKKGDLVVLVASGAGLAVGASLLR
                     I"
     gene            complement(2798..3910)
                     /locus_tag="PK105_05190"
     CDS             complement(2798..3910)
                     /locus_tag="PK105_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU61138.1"
                     /translation="MSADTRQALIDSAISLFSTKWYATVSVAEICRKARLSNGAFYRH
                     FHSKEEIFCTILDYVVDQIEKNLEVLRYHAPQDKTRSFVTIIYDFSQEHTALVRVFRE
                     GQYRLFEYERKLKSVYERAFEFVYSRHPTTAEYLFAVAGLRFASIRAAMHQLPVSTDE
                     LVSILENGVFEPQPIDLDRVFSTSITPLSIELLPDTRKRLLSEGRALFGQKGYFETNI
                     QEVTSNAGLAIGSFYRYFDSKESFYRQVIRDVGKDVRHFISINLGKEINRVERELRGL
                     WLFILFLSMDQHCYNIVREAEFVLPDEVRDYYDAFHNGYVKRDDKSSKHSSPTYVEFM
                     IGIAHYLGIEVIFNKSPEIARNTIEEIGNFYMHGLG"
     gene            4202..5215
                     /locus_tag="PK105_05195"
     CDS             4202..5215
                     /locus_tag="PK105_05195"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOU61139.1"
                     /translation="MHIGMAGIGLYLPQHFATAADLAKATSVPEDVIALKFGIKKKPI
                     ANPAETTSYMGLKAAEAALEDAKVKPEDIDLVIWCGAQHKDYPCWLAGLYVAYQLGAF
                     RAWSFDMEAMCGSMMAALDVAKSLMLGRDDLNTVLLVSGYRNNDLIDLSYPPTRFMMD
                     IGSAGSACVLKKNFGHNAILGSAFRGDGSLSEMCIVPVLGSKAWPPNPEDVHNASFKV
                     PDEKAFKQKLNDVTMPNFYAVIHEAMRKSGFKEDEIDYLAILHFKRSAHLAALQELGL
                     KEDQSTYLDEYGHLGQNDQLLSIKLGLESGKIRNGSRIAMVGAGLGFVWASTVMQWGP
                     YKE"
     gene            5316..6521
                     /locus_tag="PK105_05200"
     CDS             5316..6521
                     /locus_tag="PK105_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61140.1"
                     /translation="MKRMCLVLAVFAVFAGSAFAQGTIKIGGIWTLADITGKQGSAAA
                     QMAIEEINASGGVLGKKLELIVVDDEGKADKAAAAVEKLATVDKVEVFVGGMASGAEM
                     GKVPTYKKYGKIVMATGAAGNAVEKALGPSPGSDFYFHLHPWDYNQGASYVEGWTAIQ
                     QKYSKIKIKKIFLAYEEGAFGKSSWDATKELFGKSDYIVDGASFKSALLGGGDYTAVL
                     EAAKAFKPDFYLWAGYDADALPMLEQAKAMNFAPPIYLGAPPGWPIDFGKSKLANNVW
                     LYGMWAPSMNKTNPVSEKFYKNYIAKYKEEPATYFAPLAYDAIFILANGIKAAGTTDP
                     SSLMKALEQTKYASTLGETITFKPSEIIKHQGITRQKILQWQNGEQEVIWPFEAQTTE
                     PVYPFPAWK"
     gene            6624..7550
                     /locus_tag="PK105_05205"
     CDS             6624..7550
                     /locus_tag="PK105_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61141.1"
                     /translation="MKAKQRSNSIRAAIIAAVAIALILWKPTVLIYGLQSAGLYAAIA
                     IPMGLVLGIVHIVNLAHGEFMMLAAYAAYNACKAFGMDPLMAIIPTAAVTSLFGWVIF
                     QLTIRKALKAPELNQLILTFGLAIAFTQIINLIFTSQTYKIPLDYVSASMTIGNLTFG
                     IWSFVFVPVAIAFAYGLQYFLTKTNTGKAALAVGQNTKGAAIVGIDVYRTYGLVFAIA
                     TALVGAMGALFLTKSAIFPAVGSPFTMKSFCLIAMAGIGNIPGILGASVLLGVAESFL
                     RAFRGTRDWAEIVFFVLIIVVIMSRSLKGRKS"
     gene            7547..8482
                     /locus_tag="PK105_05210"
     CDS             7547..8482
                     /locus_tag="PK105_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61142.1"
                     /translation="MKRNASKMVALVFGIMAIMLPFFAGDYPLQVGRNIMMYMALAIS
                     WDMLLRSGQVSFGIAGFFGLGAYASILSVVHAGIPSWLSILFAAVFSGLIAFAIGLLI
                     LRLRAMYFSIVTLALGEIFRIILQNMHDFTGGPEGIVMQGVIFGGNANRLYWLTLTGL
                     CISLAGSYWFENSKIHFAITAIRNNEISAQSSGVDIFKWLLLAFVVTSAIQGLLGGIF
                     VQSYGFATPDTVFSADFMLLPIAMALLGGIYSTTGPVLGALLLGIVSEWLKLKIPYGH
                     LVVYGIMIIIVILFMPRGLKQLVRQSPKNAERGKA"
     gene            8479..9219
                     /locus_tag="PK105_05215"
     CDS             8479..9219
                     /locus_tag="PK105_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61143.1"
                     /translation="MIRLTTKNITKRFLGLVAVNNVSFSMEEGEILGIIGPNGAGKTT
                     FINLISGIIMPSEGVIEFKGKDITYMPAHERARLGIARTYQLIHPLENLTMIENIMVG
                     SIFAKGHSLKEARIIALGLCDELSLRDVDRDTSRLTILEIKKMEIARALANEPEVLFL
                     DEVMAGLNTEETKDIIAVVHKLARERNVAIGVIEHVMGVIRELTHRVMVMEAGEIIAE
                     GKYEDISKNPRVIEAYLGGATDAETTKS"
     gene            9197..9898
                     /locus_tag="PK105_05220"
     CDS             9197..9898
                     /locus_tag="PK105_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61144.1"
                     /translation="MLKLQNLECGYGRIKVLQGIDFAVGSESVGLFGPNGAGKTTLIS
                     AIMGMLKPWKGSITLEGKEIAGQETHNIARMGVALVPQERELFPGMSVEDNLILGAVY
                     IAHAKNDIPSQLEKVYQLFPILKERHAQIAGTLSGGQQRMLAIGRALMSKPRLLILDE
                     PSLGLQPSIVAEVFEVLKALKTSVSILITEQNVRESLRAIDRGYVLENGRIVLEDTAK
                     GLMTNPHVIKSYLGI"
     gene            10309..11082
                     /locus_tag="PK105_05225"
     CDS             10309..11082
                     /locus_tag="PK105_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU61145.1"
                     /translation="METDFVSVGLLSMYYMEMGTGKPIVFVHGNTASSLWYERVINIP
                     GYHGLAPDMPCFGRSSPFDEEITIDRYSESIAGFMDVKGIKDAVVVGHSLGGCVVQML
                     AINHPDLVKAMVLIDSGAPTGLVTPKDHHPFIQLMRTDRKVLEQALRAVVPTLKEEAF
                     FQKLVDEAQLMAPQAWIGNAEALSHFDISSRCSEYRGSVLVIRGALDGLITDEMAQQT
                     ARAYSKAKLVTLDGIGHSVIVEDPALFQEILQEFLAGIK"
     gene            11179..12438
                     /locus_tag="PK105_05230"
     CDS             11179..12438
                     /locus_tag="PK105_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61146.1"
                     /translation="MTNVSIIGAYNSQFGNFVKKNKETGEITDLKSIYDLILEAGKGA
                     LADANIDSHDIDGVWLGSCAPGLFANQEHLAAYATEIDPDGLRYKPMNRCEDACASGS
                     VALYNALYALEAGRAKIALVIGVEKMNLLDTKGVTHALATCSYWPEEGALGYTFPGLF
                     AEYARGYSEHYGFNSSQLAQIFATVSALNYRNGIDNPLAHFGKGALADRLGLTSADAI
                     LALPDDKNPIIAEPLRLHDCSLVTDGAAAVILVRNDISLAKSSKAVRIAGIGHVNERL
                     PLSGRTNMYELIAGKKAVQIAFAEAGITIDDVDITEVHDCFTINQLLCTEALGLSADG
                     RSGYDYLEGRFTRDDENCAINLSGGLKAKGHPVGATGVSMHALIYKQLIGQPIGATLT
                     KKQSSVGVTFNIGGSAVTNCVTVLRKD"
     gene            12518..13315
                     /locus_tag="PK105_05235"
     CDS             12518..13315
                     /locus_tag="PK105_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU61147.1"
                     /translation="MKLDEVVAVITGGASGIGEAVSKYLASLGAKIVIGDIVQESIEK
                     AVKDIETAGGSAAGLKTDVTKDAEVAALMDFAISKFGAINVVVPCAGIIRDGVMINTD
                     RETGKVKRVMETDQFRSVVEVNLIGSFITLREAARRMVDNKWTGVLFTISSINKLGQV
                     GQMNYASTKAAVAIWPKILVGEFQMKGIKGIRVMSIAPGYVGTPMVKGMNQDALADIL
                     KDVHIGRLIEPEEIAHAISMIIENEAFDGTCLEITAGLTYGSKSIAK"
BASE COUNT         3617 a         2927 c         3243 g         3758 t
ORIGIN      
        1 tcctaacttt tacagttggt atcctaaaaa gatattataa atcattatac tataatgaat
       61 agtgagaatg tcatcatgat atagactgta gtgcgtctcc taggtttttt gctttgtctg
      121 ggctaagagg tggcgaatct cctgggtagt gagcgactgc ctagaaaaat caatgccatg
      181 tcgaatttct caaattcagg tgctttttct ggtagtcggt acatgttttc ccttcaagtg
      241 cacgggtttt aatccttttc gctcgttttt cgtgcccttt atggaattac tttagcatat
      301 attctcttat attacaatgc tttagtgatt tttcagaagc ctctgcttag agccaaccga
      361 gctcttttgc cttccaggtt agctcttccc taaatttagg atgcgccacc gaaatgagtt
      421 ctcgcgctcg ctctcggagg gtcttgccac gcaggcttgc gattccatat tcagtaacca
      481 catagtccgt aaggcttcgg tgcagagtaa ctgctgtacc ttccatcaag gtgggcacaa
      541 tagtcgagat gctatcatgt tttgcggtgc tgtagcaggc tataatactt ttcccttttc
      601 catcgaaacc ttcagccgcg ccttctgctg tgtctgtttg accgcctgtt ccggaatatt
      661 gcacgggccc caggctttcg ctacccacct ggccagttag atcgacagta aggcatgtat
      721 taatcgatac catttttgaa ttctgcgcaa gtatagaagg gtgattaacc caacttccgc
      781 gcatgaattc cacggcaaca ttgtcatcga gccagtcata gagcttgcgc gaacccattg
      841 agaaagttgc tacgaatttt cctttatgaa atgctttttc actatttgtg attacgccca
      901 tctcataaag ctccatcatc gaatcgacaa tcatttccgt atggacgccc agatccttct
      961 tgtgctctaa ggcaagcgca gcagcattcg gaataccgcc aattccaagc tggattgtcg
     1021 aaccattttc gataaggtcg ccgatataat tccctatagt gaggtccaat tcgtttggtt
     1081 gcggagaggg ctgttcaggg actggttgat cgcactccac gaaataatca acttcagaag
     1141 tatgaagatg agtatcgcca aaggtacgtg gcaagcgtgg attgacttcc aggataacta
     1201 gatcagaggc ctccagaatg tctttttcat aggtaatacc cagagaaagc gacacaaacc
     1261 catgcttgtc tggaggtgta caggttcccc agaaaatatt tggcttgcgt gcatagataa
     1321 aatccgctgc cgcacgatgg agcatattgg gcacataagt tacagttcct acattggctt
     1381 taagggcagc tcttggtcct ggagcatgaa accagcttgc cagttcgaaa tgacccttca
     1441 tttccggttt catatagaaa tcgtaaggct taagagtgag gcaggaaaaa accctaacat
     1501 tttccacttt atcagcgact gtatggaatt gcatcatgct gccttgtggc tctgatgcac
     1561 acattgctac aacgatgttg tcgttgcttt tgatttttga aacagcttcg ggaatcgaaa
     1621 tgagcttccg attgtattca tcttgcgaat tcataaaacc tccatattct tagctgccca
     1681 tgcgtttgct gtgctatgga tctgatccat agcgcttggg ctacgactca atcgcgggct
     1741 atgaaagact catatcctca gaagcgacgc acccactgca agacccgcac ccgaggctac
     1801 aaggacgacg aggtcgcctt ttttgatgcg gccttcttgt aaaccgacat gcatagccat
     1861 gggaatacat cccgaacctg tgtagccata ccgatccatc accattgtcg ttttttcgat
     1921 tggctcccca aggatctcca taacctgttc gataaccgat ttattaatct gggtgaagag
     1981 gtaatgatcc acttcggatt ggggcaagtg gaccttatcg agtaggtgct ggatcagggg
     2041 tggccaaagt tttatattac ggtcgcccgg cattttttgc aggagctgaa ggccatactc
     2101 gcctttatta agccgctcat gcgtgattgg aaatcgcgtt ccacctgcat agacacccag
     2161 gaaattccac tgcgttccat cagtgataaa tgtgccgtca atgtagcctg aattgctttc
     2221 ctcttgcctt tcgagcacca ccgctcctgc tccatcagcg aaaatcgacc atccaaaaga
     2281 atcatcgtct ctaatgaagg caggcatatt gtatacgcca atgaccaacg catagtggac
     2341 agaagtatcg gtcattagat tccgcgctgc tacatcaagg gcttcgacaa agcttgcaca
     2401 ggaagcgcca agatcgaagc tctttgcgta tgtctgccct ccttgtagtc ggccttgaac
     2461 aataatgctt gtagcagggg agatatgttc aggagtatcg gttgccacga tgaaaagccc
     2521 aatatcctct gcgtcaattt tggcagcttt aagcgcatct cgggctgctt tttctgcaaa
     2581 atcagcggtt gtctcctgca gaccagagag acgggagata tgtctttgct tgatgcctat
     2641 cttttgttca tgacgggctg catcgaactc aatacctgtc aatttcatga cagtcttgtt
     2701 gtcaataggg gcaccaggca catagattcc tgtgccaaca atcactgctt ttgccatata
     2761 atccttcctt aattaagaag attatttccg cataaaccta gcctaatcca tgcatataga
     2821 agttcccgat ttcttcaatt gtgttgcgag cgatttcagg agatttgttg aagatgactt
     2881 ctatgcctaa atagtgtgct atgcctatca tgaattcgac ataagttgga ctactatgct
     2941 tgcttgattt atcatcccgc tttacatagc cgttgtggaa agcatcatag taatctcgta
     3001 cctcatctgg aagtacgaat tccgcttcgc gtactatgtt gtagcaatgt tgatccattg
     3061 aaagaaacaa aataaagagc cataaacctc gcaactctcg ttctacacga ttgatttctt
     3121 tgccaaggtt tatgcttata aaatggcgta catctttgcc tacatctcga atcacttgtc
     3181 ggtaaaatga ttctttgctg tcgaagtagc gataaaatga gccaatagcg agcccggcgt
     3241 ttgatgtcac ttcttgtatg ttggtttcaa aatagccctt ttggccaaaa agcgctcgac
     3301 cttctgagag cagacgtttg cgcgtatctg gcaacagctc tatggagagt ggagtaattg
     3361 aggttgaaaa caccctatct aaatcgattg gctgcggctc gaaaacgcca ttttccagaa
     3421 tagaaacaag ctcatcggta ctcacaggaa gctgatgcat tgcagcacgg atgcttgcaa
     3481 atctcaatcc tgcaactgca aagaggtatt ctgcggtggt cggatgcctg gaataaacga
     3541 attcaaaagc tctttcgtag acgctcttaa gcttgcgttc atattcgaaa agtcggtatt
     3601 gcccttctct gaatacccgt acaagagcag tatgctcttg agaaaaatcg tagatgattg
     3661 tgacaaagct cctggtttta tcctggggcg catggtaccg caaaacctct agattttttt
     3721 cgatttgatc tactacataa tcgagaattg tacaaaaaat ctcttctttg ctatgaaaat
     3781 gcctatagaa tgctccattg gagagtcttg cctttcgaca tatctctgca acagagacgg
     3841 tggcatacca ttttgtggag aaaagagaga tagcgctatc gatgagcgct tgacgtgtat
     3901 ctgcactcat tcacatggcc tctggtaaga agcgattcac atcttactac atttgagtat
     3961 actctcgttt atgcctctat gcaagcgaaa tttatcaaat atcgaaaaat taggcagatt
     4021 tccggttatg acatctattt gatgatataa acaataaata aggtaatcat cataaacata
     4081 tcgatgatat ctacagagaa ttatcatttt tgcccttgac ggattagaaa aagtgacaca
     4141 caatattgta agaggtgatt cagctcttac agatgcaaat acattttctt aggagcgtat
     4201 gatgcatatc ggaatggcgg gtattggact ctatttacca caacattttg caacagccgc
     4261 agatctcgca aaggctacct cggtacctga agatgtaatt gccttgaaat tcggaattaa
     4321 aaagaaacct attgccaatc cagcggaaac aacaagctat atgggcttaa aagctgcgga
     4381 agcagcgctc gaagacgcta aggtaaaacc cgaggatatc gatttggtga tttggtgcgg
     4441 tgctcagcat aaagactacc cttgttggtt ggctgggctc tatgtggcat atcagcttgg
     4501 cgcgtttcgg gcatggagct ttgatatgga ggcgatgtgt ggctccatga tggcagctct
     4561 cgatgtcgct aagtcgctca tgctggggcg ggatgatctg aatacggtgc ttctcgtttc
     4621 tggatataga aataacgacc tcatcgatct ttcatatcct ccaacccgat tcatgatgga
     4681 tattggttcg gctggatcag cttgtgttct caaaaagaat ttcgggcaca acgctatatt
     4741 agggtccgca tttagaggag atggctcgct ttcagagatg tgcatcgtgc cggtactcgg
     4801 ctcaaaagca tggccgccga atccagaaga tgtccataat gcatctttta aggtacccga
     4861 cgagaaggcc ttcaagcaga aactaaacga tgtcacaatg cccaattttt atgctgttat
     4921 tcacgaagca atgaggaaaa gtggcttcaa agaggatgag atcgattatc ttgccattct
     4981 gcatttcaag cgaagtgcgc acctggcagc gcttcaggag ctaggtctta aagaagatca
     5041 gtcgacctat cttgatgagt acgggcatct gggacaaaac gaccagcttc tgtcaataaa
     5101 gctggggttg gagtcgggaa agattagaaa cgggagccgt atcgctatgg taggagcggg
     5161 actcggtttt gtgtgggcga gcacggttat gcaatggggt ccctataaag agtgatctat
     5221 tacaatctgg ccaatggatc cggttacttc cgggcggttt tatccgcgcc ggttaaaatt
     5281 gccgcagtat tgaaatgcgg tataaggagg cattcatgaa acgaatgtgt ttagtattgg
     5341 cagtgtttgc tgtttttgcg ggcagtgcct ttgcgcaagg caccataaaa atcggtggca
     5401 tttggacgct ggctgatatc acgggcaaac aaggaagcgc agccgcgcaa atggcaatag
     5461 aagagataaa tgcttcaggc ggcgttttgg gcaaaaagct cgaacttatt gtggtagatg
     5521 atgaaggtaa agccgataag gctgccgcag cggtagaaaa gcttgccact gtcgataagg
     5581 tcgaagtctt tgtcggtggc atggcatctg gcgcagagat gggcaaggtg cccacctata
     5641 agaaatatgg aaaaattgtc atggctactg gtgccgcagg caatgctgtt gaaaaagcgc
     5701 ttggtcccag cccggggtcg gatttttatt tccatcttca tccatgggac tacaaccagg
     5761 gagcttccta tgtcgaagga tggactgcca ttcagcagaa atattcgaag atcaaaataa
     5821 agaagatatt tctagcttat gaagagggag ctttcggaaa atcctcatgg gatgcgacaa
     5881 aggagctatt tggaaagagc gattatatcg tggatggtgc ttcttttaag agtgcacttc
     5941 tgggtggcgg cgattatacc gctgtgctcg aggcagcaaa agcctttaag ccagatttct
     6001 atctctgggc aggttacgat gcagatgcac ttccaatgct cgagcaagca aaagccatga
     6061 attttgcgcc accaatctac cttggagctc ctcccggttg gcctatcgat ttcggaaaat
     6121 ccaagcttgc gaataatgtt tggctctatg gcatgtgggc tccatcgatg aacaagacca
     6181 acccagtcag tgagaagttc tacaaaaatt acattgctaa atataaagaa gaacctgcaa
     6241 cctattttgc gccccttgct tacgatgcaa tctttatcct tgctaatggt atcaaggctg
     6301 ctggtactac agatccttct tctctaatga aggcgcttga acagactaaa tatgcatcta
     6361 cactgggaga aacaataacg tttaagccat ctgagatcat caagcatcaa ggcattacgc
     6421 ggcagaaaat acttcagtgg cagaatggcg aacaagaagt aatctggcca tttgaggcac
     6481 agacgacaga gcctgtctac ccattccccg cgtggaaata gccttttaaa tcaggcttga
     6541 aatcgtagtc taaagcaccg ccgccataag ggacctattg taccccctac gcggcggtaa
     6601 cttttgaaaa acaggcggaa aagatgaaag caaaacaacg aagtaattcg atccgcgccg
     6661 caattattgc tgcagtggcg attgctctaa tattgtggaa accaaccgtg ctcatctatg
     6721 gactgcaatc cgcagggctc tatgcggcta ttgcgattcc catgggtctc gtgctcggca
     6781 ttgttcatat tgtaaacctt gctcatggcg aatttatgat gctcgcagcc tatgctgcgt
     6841 acaatgcatg caaggctttt ggcatggatc ctcttatggc gataattcct actgcagctg
     6901 taacttcgct gtttggttgg gtcatttttc agcttacaat tcgcaaggcg ctaaaagccc
     6961 ccgagctcaa tcagctcatt ttaacatttg ggcttgccat cgcattcact cagatcatca
     7021 atcttatctt tacttcgcag acctataaaa ttccactcga ttatgtatct gcctcgatga
     7081 ccattggtaa tcttactttt gggatatggt cctttgtctt cgtgccagtt gcgatagcct
     7141 tcgcgtacgg cctgcaatat ttcctgacaa agacaaatac tggtaaagct gctcttgccg
     7201 ttggccagaa tacaaaggga gcagcaatag ttggtattga tgtctaccgg acatatggat
     7261 tggtattcgc aattgccact gccctcgtgg gggctatggg agcattattc cttactaaat
     7321 cagcaatttt ccctgcagtt ggctctcctt ttactatgaa gagcttttgc cttattgcca
     7381 tggctggaat cgggaatata cctggaattc taggcgcaag tgttcttcta ggggttgcag
     7441 agagctttct ccgggctttt cgcggtacgc gtgactgggc tgaaattgtc ttctttgttt
     7501 tgatcattgt ggtcatcatg agtcgttcat tgaaaggaag gaaatcatga agcgcaacgc
     7561 atcaaagatg gtcgcattgg tttttgggat aatggcaata atgcttcctt ttttcgctgg
     7621 ggattatccg cttcaagttg gccgtaacat aatgatgtat atggcactcg caattagctg
     7681 ggatatgttg cttcgctctg ggcaggtatc ttttggcatt gcaggattct ttgggctagg
     7741 tgcgtacgca tcgatcttga gtgtcgtaca tgctggtatt ccatcatggc tttcgattct
     7801 attcgcggca gttttctccg ggcttatagc atttgctatc ggtttgctta ttttgcgcct
     7861 tagagcgatg tatttttcga ttgtgacgct tgcgctgggt gaaatcttcc ggatcatact
     7921 tcaaaatatg catgatttta ccggagggcc agaaggcata gtcatgcagg gagtcatctt
     7981 tgggggaaat gccaatagac tgtattggct cacgcttacc ggtctgtgta tctctcttgc
     8041 tggatcatat tggttcgaaa acagcaagat ccattttgct atcaccgcaa tacgcaacaa
     8101 cgagatttct gcacagtctt ccggagtaga tatcttcaaa tggctgctcc tggctttcgt
     8161 ggtcacctcc gcaatccagg gcttgcttgg tggcatcttt gtacaatctt atggatttgc
     8221 aacaccagat acagtgtttt ctgctgattt catgcttttg cctattgcta tggcattgct
     8281 aggcggtatc tacagtacta ctggtccagt ccttggtgcc cttttgcttg gtattgtatc
     8341 tgaatggctc aaattgaaaa ttccatatgg ccatcttgtt gtctatggca tcatgatcat
     8401 aattgtaatt ctctttatgc ctcgggggct caaacagctt gttcgacaga gtcccaaaaa
     8461 tgcagagaga gggaaggcat gataagactc acaacgaaaa acatcaccaa gcgttttcta
     8521 ggcctcgttg ctgtgaacaa cgtgtcgttt agcatggaag aaggcgagat tttaggaatt
     8581 attggcccaa atggcgctgg aaagacgaca tttatcaacc tcatatccgg aatcattatg
     8641 ccaagcgaag gagttatcga attcaaaggg aaagacatca cctacatgcc agcccatgaa
     8701 agggcaaggc tcggtattgc aagaacttac cagcttatcc atcctcttga aaatcttacg
     8761 atgatagaga atatcatggt aggatcgata tttgcgaaag ggcatagcct taaagaagct
     8821 cgcataatag ctctaggact ctgcgatgaa ctaagtttgc gcgatgtaga tagggataca
     8881 agtcgcctga cgatcctcga aataaagaaa atggaaatcg cccgtgcgct tgcaaacgaa
     8941 ccagaggtgc tctttttgga tgaggtgatg gctggtctga atacagaaga gacaaaagac
     9001 atcattgccg tggtgcataa acttgcgcgc gagcgaaatg ttgctattgg cgttatcgag
     9061 catgtgatgg gtgtaatccg tgagctcacc catcgtgtca tggttatgga agccggagaa
     9121 atcatcgctg aaggcaaata cgaggatata tccaaaaatc cgcgtgtcat agaagcctac
     9181 ctaggaggag ccaccgatgc tgaaactaca aaatcttgaa tgtggttatg gccgaataaa
     9241 agttctgcaa gggatcgatt ttgcagtcgg ttcggaatcg gtgggccttt ttggtcctaa
     9301 cggcgcaggc aagacgacct tgataagcgc tattatgggt atgctgaagc cctggaaggg
     9361 gagcattaca ttagaaggca aagaaatcgc tggccaggag acccataata tcgcaagaat
     9421 gggcgtagct ctcgtgcctc aggagagaga actttttcct ggcatgtccg tcgaggacaa
     9481 ccttatactc ggcgcagtct atattgctca tgcaaagaac gacattccat cacaactgga
     9541 aaaagtctac cagctatttc ctattctgaa agagcgccat gcgcagatag cgggtactct
     9601 ttcaggcggt caacaacgca tgcttgctat agggagagcg ctcatgtcga aacccagact
     9661 cctaattttg gatgaaccgt cgctgggttt acagccttcg attgtcgccg aagtgtttga
     9721 ggttcttaag gcactcaaaa cttcggtttc catattgatt acagagcaga acgtgcgaga
     9781 aagtttgcgt gctatagatc gaggttatgt cttagaaaat gggcgaattg tgctcgagga
     9841 taccgccaag ggtcttatga caaaccctca tgttatcaaa tcatatttgg ggatctaggg
     9901 gcatttaatg gcaaggcaaa agatgaatgg aatcaagata gccttcgggt tttttggtaa
     9961 acaagtcgaa ttgttatctg gggttccaat tatctttctc gatggtattg cgatgtcgat
    10021 ctctcattgg aggtctctta ttgggatacc tccctcatgt actccttgcc tctgcaacga
    10081 ctttcaaggc cagatgcata gcgaaagagc aagaggacta tctttgctta ccctcaactc
    10141 agaaaacctc gtatctcgat atttttgtgg cgagataagc ggggaggtcg acactcccat
    10201 cagaatgatc tctggattta ttaaaatatg tcagtgtctt cagggtatca attaaaacat
    10261 cagccacgct cgggtaaatg agccgactga atccactatg gaggaaatat ggaaactgat
    10321 ttcgtctctg taggacttct ttccatgtac tacatggaaa tgggaacagg aaagcccatt
    10381 gtctttgtgc atgggaatac agcttcatca ttgtggtatg agcgcgtaat caatatacct
    10441 ggctaccatg ggttagcgcc agatatgcca tgttttgggc gttcatcgcc ctttgatgag
    10501 gagatcacca ttgatcgcta ttctgagtcg atagcaggct ttatggatgt taagggcatc
    10561 aaggatgccg ttgtggtggg tcattcatta gggggctgtg tggtccagat gcttgcgatc
    10621 aaccatccgg atctagtaaa ggcaatggtg cttatagatt cgggagcgcc tacgggcctt
    10681 gtcaccccaa aagaccacca tccttttatc cagctcatgc gaacagatag aaaagtgctt
    10741 gaacaagcac taagagccgt tgtacctaca ttaaaagaag aagcattttt ccaaaagctt
    10801 gtagatgagg cgcagctcat ggcgccacaa gcttggatcg ggaatgcaga agcactgtcg
    10861 catttcgaca tcagctcaag atgctctgaa tatcgaggaa gcgttcttgt aattcgaggt
    10921 gcgctagatg ggctcattac tgatgaaatg gcacaacaga ctgcccgcgc ctattcaaaa
    10981 gctaagcttg tgacactgga tggcataggg cattcggtga tagttgaaga cccggcacta
    11041 tttcaagaga ttcttcaaga attccttgcg ggaataaaat gacatagtgc tgcattatgc
    11101 aattatttgc tgtcgtcaat atctttaccg gaaaaatgca agcgaatatc acagagccgc
    11161 aacggaagga aaatcagtat gactaatgta agtataattg gtgcatataa ttcgcaattt
    11221 ggtaattttg tcaaaaagaa caaagagacg ggagaaatca cggatctcaa atcgatctac
    11281 gatctcatat tagaagctgg caaaggagca ctcgcagatg cgaatatcga tagccacgat
    11341 atcgatggtg tatggcttgg gtcttgtgct ccaggccttt ttgccaatca ggaacacctg
    11401 gctgcatatg ctactgagat cgatcctgat ggattgcgtt ataaaccaat gaatcgatgc
    11461 gaagatgctt gtgcctcagg ttccgttgct ctctataatg cactttatgc ccttgaggcg
    11521 gggagagcga agatcgctct tgtaatcggc gtagagaaga tgaaccttct cgatacaaag
    11581 ggcgtgactc atgcattagc cacttgttcg tattggcctg aagagggagc tttgggttat
    11641 actttccccg gtctgtttgc tgagtatgca agaggctatt cggaacacta tggcttcaat
    11701 tcctcccagc ttgcacagat attcgcgacc gtatcagcgc ttaattaccg taatggaatc
    11761 gacaatccac ttgcacactt tggcaagggt gccttggctg atcgtctagg ccttactagc
    11821 gcagacgcta ttctagcgct tccagatgat aaaaacccca taattgccga gcccttgagg
    11881 ctgcacgatt gttcgcttgt taccgatgga gcagcagcgg tgatattggt gcgcaatgat
    11941 atatcgctcg cgaaatcttc aaaggcagtg agaatagcag gcatagggca tgtaaacgaa
    12001 cgcctaccct tgtccgggcg cacgaacatg tacgaactta ttgcgggcaa aaaagctgta
    12061 cagatagcat ttgcggaagc gggtatcacc attgatgatg tcgatattac ggaagttcac
    12121 gattgcttta caatcaatca gcttttgtgc accgaagcac ttggcctatc tgcagatggc
    12181 cgttctggat atgactacct agaaggtcgc tttactcgag atgatgaaaa ctgcgctatc
    12241 aatctttctg gaggcctaaa agcaaaaggc catccagtcg gtgcaaccgg tgtctcaatg
    12301 catgcgctta tttataaaca gctcattggt cagcccattg gcgcgacact caccaagaag
    12361 cagtcgtcag ttggtgtgac ttttaatatt ggaggttctg ctgtaacgaa ttgcgttact
    12421 gtcttgagaa aagactaatt taggctttat gaattttgca taacgatatt atctcatttt
    12481 catcaagagt tactgttagc aagggaggga aattagcatg aaactagacg aggtagttgc
    12541 tgtgattacc ggtggcgcga gcggtattgg cgaagcggtt tccaaatatc ttgcctcttt
    12601 gggtgcgaag atagtcatcg gcgatattgt tcaagaaagc atcgagaaag cggttaaaga
    12661 catcgaaaca gctggaggaa gtgccgctgg cctaaagacc gatgtaacca aagatgctga
    12721 agtggcagcg ctcatggatt tcgctatctc gaaatttgga gcaatcaacg ttgtagtgcc
    12781 ttgtgccgga ataattcgcg atggagtcat gataaacacc gatagagaga caggtaaagt
    12841 caagcgagtt atggaaaccg atcagtttcg atcggttgtc gaagtgaatc taatcggctc
    12901 ctttattaca ttgcgggaag ccgcgcgccg aatggtagat aataaatgga caggtgtgct
    12961 ttttaccata tcaagtatca ataagctagg ccaggtagga cagatgaatt atgcttctac
    13021 aaaagcagcc gtcgcaatct ggccaaaaat ccttgttggt gaatttcaga tgaaaggaat
    13081 taagggaatt cgcgttatga gcattgctcc aggctatgtc ggtactccta tggtcaaagg
    13141 aatgaatcaa gatgctctag ccgatattct gaaggacgta catataggaa gacttattga
    13201 accggaagag attgcacacg cgattagtat gatcattgaa aatgaggctt ttgatggcac
    13261 gtgccttgag ataacggctg ggctaaccta tggctcaaaa tcaattgcaa agtgatgttg
    13321 atggatcgtg gccaagaaag ctgagctccg gtatgttctt atgagcatca attaagaggc
    13381 taaatactat cagctcttag gattacaaga agcgtatctt agcattggat cgggatgata
    13441 acatgatgaa gaaggaagcg catcatgtta tcttaacatt ttcttccgac ttaccaattg
    13501 ttccgctttt tcggctatgg cgtccgcatc catgccaaat ttttt
//
LOCUS       DAOOWY010000050        13391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4637_length_13391_cov_10.018671, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000050 DAOOWY010000000
VERSION     DAOOWY010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 13391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13391
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4637_length_13391_cov_10.018671"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /gene="nusA"
                     /locus_tag="PK105_05240"
     CDS             <1..1367
                     /gene="nusA"
                     /locus_tag="PK105_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOU61148.1"
                     /translation="KTLEEAILAAYKKKFGTIDNAIVRMKENYEGIEIYAKKTIVSDE
                     DFEDEVLEIPLEEAKKFADEAEIGDILEISIDPSEFDFQSVMLAKQTARQSLRDISRD
                     TLYAEFKSKVGEIVIGYFQRERNGTIFVDLGKIEGIFPRKYQSPRETYHVGDRIKALI
                     VEVEKTKTGFQVVLSRTHAEFVRKLLELEIPEIYDKTIEIFKIVREPGYRTKVAVYAK
                     RSDVDPVGACVGPKGMRSQLISQELEGEKIDFIRYDINPKEFIKNALAPAQVKEVYIL
                     DEAKHMALAVVDEHELSIAIGKSGQNVKLANRLCDWNIDVMTEEQYLQDSRNIELKKA
                     ADRLFSVEEEEGGEFLIRDLPNLEQTWIDALESAGIRLIEQFLELDEEEIARNTALSA
                     TDISAIRDIIEDNVEIIQEEVEEEGIVSYSCPECGSPITIDMDHCPSCGVALSFEAEE
                     EQDE"
     gene            1377..4124
                     /gene="infB"
                     /locus_tag="PK105_05245"
     CDS             1377..4124
                     /gene="infB"
                     /locus_tag="PK105_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU61149.1"
                     /translation="MTESNDKDLKKVHLIKQPRQDSPEQPQEMSAQNPLDQGAEKKKV
                     VVVVKKKTITTKKVQAKAMIPGTPKDQKIDEEQSALSTQPHSEVGQNKAQIAKEDQVA
                     SPSPITPSTTTLPSSKSPSNVPSMKLSEHKQEIGQDETIISSQRRYSSDGRAGNLAQG
                     RPSSTFRNSHDRGSSRPANGSYQGRAYGQPSGRSYPSSSSRSGQSGSQQFSRGNRPSG
                     GFQSQGGTGSGGRTAYPYAKTGQPGSGQRPGGYIGARQGPAGGRYGLRQTPAPVPSTG
                     NERPASRRAPVKKKGVFNKREEETELEKQIQLKKKAEAKLAAVPKSIDIMENISISEL
                     AKKMNLKPADLISKLMSLGVMATINQKIDADTAAILAAEYSCEVKVVSLYDETVIEKA
                     VDRPEELRPRPPIVTVMGHVDHGKTKLLDAIRKTDVVSQEFGGITQHIGAYMAEYPNG
                     KISFLDTPGHAAFTKMRARGANITDIVVLVVSATEGVMMQTKEAIDHAKAANVPIIVA
                     INKIDLPEANPDRVKTQLSELGLIPEEWGGQTQFVEVSALQKIGIRELFDAILLQAEM
                     LDLKANYDRLAEGKIIESRIDQGRGIVATLMVQNGTLHVGDPFVAGIFPGRVRAMFDD
                     KGNRIEQATPSMPVEVIGFEGMPEAGDPFEVVEDEKFARQISTKRQELKKYEEGRNVK
                     KVTLDNLYETISQGEIKELKVIIKGDVHGSVEALKGMLEKLSTKEVHLNVIRAAAGAI
                     TEDDVMMASASNAIIVGFNVRPTSNAKQLAEREKVDIRKYNLIYRAQEEIKRAMEGLL
                     APELKEQEVGKAEVRNIFRVPKAGIVAGCFVSEGIVKRNCQVRVIRDNIEIFQGNLSS
                     LKRFKDDVKEVAAGYECGIGIENCNDLKEGDVLEFYETIEVARTLESGDKNEPGAQAS
                     S"
     gene            4096..4446
                     /gene="rbfA"
                     /locus_tag="PK105_05250"
     CDS             4096..4446
                     /gene="rbfA"
                     /locus_tag="PK105_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOU61150.1"
                     /translation="MNQVRKRRLEELIREEISKMITFGEIKDPRVNSFLSITRVEASQ
                     DGSHAKVFVSSLDDIEGHLDEAIVGLSHAAGFVQSLIAKRVRLRLTPVLTFVADHSLK
                     EGFELTEKLKDLLK"
     gene            4446..5381
                     /gene="truB"
                     /locus_tag="PK105_05255"
     CDS             4446..5381
                     /gene="truB"
                     /locus_tag="PK105_05255"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOU61151.1"
                     /translation="MQHSGFFLVDKPKGFTSNRVLQDIRKSLSVQSSAYPQKYGHAGT
                     LDSFASGLLIILTGKITRLSRWFMHQNKEYEAVFRFGEETDTLDPQGKIMAIGELPAR
                     ETLERIIPSFCGNIEQIPPSYSAVHVGGERSYKIARAGNKPEISPRSIIIHDIELESF
                     QEKEATLRIRCSSGTYIRSLARDIGHACGSCATVISLRRTVIGPFSISEAHSPQECNE
                     KSIRVFSPDIAQSLGLATAVIKLDFVEHFIHGGTLPAKAVTLDCDQMTLFRNPDFSFE
                     SKPVALFSEKGELLGIAEQKDKEYAPRVVMAEEPR"
     gene            5381..6187
                     /locus_tag="PK105_05260"
     CDS             5381..6187
                     /locus_tag="PK105_05260"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HOU61152.1"
                     /translation="MKVLTWQEFQNNHSQEKLAATVGVFDGLHYGHQVLIDTIKLQSP
                     ALKPCIITFKENPKKILNPSSYKGSLLTLEQKLSGFQHMGIEYCVMIDFSDNFAKLSG
                     REFLAALYIANVQFIAVGVNFQFGHNLDTNAAKVEKLSEEIGLRSCIVENVMYRGHAI
                     SSSRIRHAVLEGRLGEAAEMIGRPYQVLARRETSVIDEKAEFFLPEDDLLIPPEGQYE
                     VFIKDNDTVQKTKVNVVHNYIALSLRMRSDNIRLAFVDKAVQQKENVVWL"
     gene            6178..6447
                     /gene="rpsO"
                     /locus_tag="PK105_05265"
     CDS             6178..6447
                     /gene="rpsO"
                     /locus_tag="PK105_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOU61153.1"
                     /translation="MALSKEDKAQLVLEYGKDSKNTGAIETQVAIISARIAYLTEHFK
                     THKKDTNSRRGLLKLVGQRRKLLKYLQRTDLESYRAIMEKLQIRK"
     gene            6516..8696
                     /gene="pnp"
                     /locus_tag="PK105_05270"
     CDS             6516..8696
                     /gene="pnp"
                     /locus_tag="PK105_05270"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOU61154.1"
                     /translation="MINKLECKIGNENLVFETGRMAKQANGAVFATYGGSAVIATVCC
                     SPTPIEGLDFVPLTVEYSEKYYAAGKIPGGFIKRETKPKDREILVSRIIDRPMRPLFH
                     KEFGREIQIVPTCISADQINPPDVIAANAASAAIHISDIPFDGPVGCVRVALVEGSYI
                     LNPTYEQIDHAKLEIVVAGTAQGITMVEGGSHEASEEEMIQAIEMAREPISQICSTIE
                     ELRRIAGKEKLAPAPFSIELNNKDEIRKCAYELLSRALFTKIKQERAKAVSEAISAVK
                     EKFADDLDEEIQMKLFSKLMDDLQYEILRSSILDKGLRVDGRGLEEIRPITCEIGVLP
                     RTHGSALFTRGETQVLAVTTLGTVFDEQIFDDIEGDRRERFMLHYNFPPFAVGEVGRL
                     GTGRREIGHGDLARRSIEPMLPPKERFPYTIRVVAEVLESNGSSSMATVCSSSMSLMH
                     AGVPFTKPVAGIAMGLVTDDTRYAVLSDILGDEDHLGDMDFKVAGTKDGITGFQMDIK
                     ISSVSTEILRKALDQAKCGRLHILSIMEKTIAEPQSEISPYAPQVINARIDPEKIGLV
                     IGPAGKNIKALSEKYDVQINIDEDGYLTVYGKDQKSTYSARDAILGMVEEPEVGKIYN
                     GTVKRIMDFGAFIEILPGREGLCHISKLSKEHVDKVSDLLKEGDIIKVKLMEIDHLGR
                     LNLAAVDALDDKGQIAQRDARRNDRPSDRQRDMRSSRRDSHWDR"
     gene            8807..9262
                     /gene="dut"
                     /locus_tag="PK105_05275"
     CDS             8807..9262
                     /gene="dut"
                     /locus_tag="PK105_05275"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOU61155.1"
                     /translation="MVKPEDRAIEVLIALSEGARIPQYATDNAAGADLHAVLQKPLVL
                     EPLSRALISTGLRIALPSGYEAQIRPRSGWAYRHGVTCLNAPGTIDSDYRGEIKVILI
                     NLSDKPFVIENGDRIAQLVIAPVVRADFSISSHLDDTTRGAGGFGSTGR"
     gene            9288..10625
                     /locus_tag="PK105_05280"
     CDS             9288..10625
                     /locus_tag="PK105_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU61156.1"
                     /translation="MKERPSFTLWRITISEIMTSFLGTFLFFFIVFLINQVLLFAEDI
                     LSRGADLLSVVKLLFYSLPTILAITIPFSILAATLMTSSRQNSDNELLASSSLGIRPL
                     WLYVPFLIIGLAISIGSFCFNEWSIPRAAQGFKNEYAELIKKSAKIELAPYSISKYGD
                     KLLVTGGSEEGSSTSRLQDVLIIDQSGGYDSDTVIADDVGIEFSKDSLMAILSMNSLT
                     ELKRLNDASAGDFSVTMAKSAEIRIRIREPLMNYSSTAPSEMSLTILAKKIKEKERIL
                     DDRIKKKDFQKDTALDRLRLNYDLLAIETQQERSLKTKGSLTSLTLLQNDLISLTNKI
                     KSLENQKINDTSLQIYRLEYEKKLVLPSACFFFALLALPLGIGSKRAGRAAGFGIALL
                     LSVIYWALLFLGQTLGYRQNVNPVFSMWMPNLVMLLATISLWVVRKIKTGHFL"
     gene            10652..11767
                     /locus_tag="PK105_05285"
     CDS             10652..11767
                     /locus_tag="PK105_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU61157.1"
                     /translation="MAQQSSAYQQDTRFLPIILWRALLGKVFIWTIGGTLFLSLVISL
                     VELFSLLWKFLAQNALFWDIIFWISLGITDHIVDALPVAFLFAIVFVLSDWHANHELE
                     AVFSAGLSLQRLLIPILSISILFCAAEFYLTDFASIPFSRSRNDLQSRILKESAGKQT
                     VPGLIVDSGKQVYTYRYFDEKNLRLYDVSVIERDAYGNLTRKISAESAKWEQGTWVFA
                     NSIIYSRQDNDWTFSKFEEYSEPVINEPPSSFTRPSMNPRFLTMRELNNHIEFLKASG
                     LPVVDAEVEKQKRLSFSLTPLIVVGLAAAFSGRFKKSVFLLSLLLSLCSATLYYVAQM
                     IASVSARSGLISPRISMWAVIIVFGALSTISYLSART"
     gene            11807..12958
                     /gene="tgt"
                     /locus_tag="PK105_05290"
     CDS             11807..12958
                     /gene="tgt"
                     /locus_tag="PK105_05290"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU61158.1"
                     /translation="MIKSIFQEKHRDPSCSARTGLISLPHGDVQTPAFMPVGTNATVK
                     AVEPRDLESMGFSLILSNTYHLFLRPGPEVISKAGGLHGFSGWQRNFLTDSGGFQIFS
                     LSQFRKILDDGVRFRSHIDGSSHILTPENVVDIQTAFNSDIQMALDICAPWGESENKA
                     YKATALTYDWAKRAKTQWLCQRERGYQGYLFGIVQGNFYRELRKMSVEQIASLDLPGI
                     AIGGLSIGEPKEAYMEYLDFTAALLPSDKPRYIMGIGTPDYIIEAVRNGIDIFDCVYP
                     TRTARNGLLFTSRGQIVIKKAIYKKDFRPIDTDCSCRVCKTYSRAYLHHLFRNNEILY
                     SMLASQHNLHFMADFASKIRQAIQEDGFESFARAFLDRYTGMEEKEEAQ"
     gene            complement(12971..>13391)
                     /gene="greA"
                     /locus_tag="PK105_05295"
     CDS             complement(12971..>13391)
                     /gene="greA"
                     /locus_tag="PK105_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOU61159.1"
                     /translation="IMEVDVPANQKEIAYALSLGDLRENAEYKAAKERQDELNAKVGK
                     LKSELDRAQIIDSDQVDSTSISFGTKIALKNLITGEDEFFTILGPWESDPEHQVISYL
                     SPLGKHLLNHKTGEKLNFTINERKFSYEVVKVEKADF"
BASE COUNT         4005 a         2668 c         3147 g         3571 t
ORIGIN      
        1 ttaaaacgct cgaagaggcg attctagcag cctacaagaa aaaattcgga accatcgata
       61 atgctatagt ccgaatgaag gaaaattatg aagggatcga aatctatgca aaaaagacta
      121 tagtctcgga tgaagatttt gaggatgaag tcttggagat tcctcttgag gaagccaaga
      181 agttcgcaga cgaagccgaa atcggagata ttctagaaat ctcaatcgat ccatctgagt
      241 tcgattttca atcggttatg cttgcaaaac agacagctcg acagagcctg agggatattt
      301 ctcgcgacac tctatatgca gagtttaaat cgaaagtagg agaaatagtc attggctatt
      361 tccagcgaga gcgaaacggg acgatatttg ttgatttagg caagattgaa ggtattttcc
      421 cgcggaagta ccagtctcca agagaaacat atcatgtggg cgatagaatt aaagccttaa
      481 ttgttgaagt ggaaaagacc aagactggat ttcaggttgt cctttcaagg actcatgcag
      541 aatttgtacg caagttgctg gaactggaga taccggagat ctacgacaag accattgaga
      601 tattcaaaat tgtccgggag cccggctata gaacgaaagt agctgtatat gcgaaacgaa
      661 gcgatgtgga tccggtaggt gcttgtgtag gtccaaaggg catgcggagt caactgatca
      721 gccaagagct tgagggtgaa aaaatcgact ttattcgata tgatataaac ccaaaagagt
      781 ttattaagaa tgctcttgcg cccgcacagg taaaagaagt atatatactg gacgaagcca
      841 aacacatggc gctagccgta gtcgatgagc atgaactttc catcgctatt ggaaagtcag
      901 ggcagaatgt caagctagca aatcggctat gtgactggaa tatagatgtc atgacagagg
      961 aacagtattt gcaggactcc agaaatatcg agttaaaaaa ggccgcagac cgtctatttt
     1021 ctgtagaaga ggaagaaggt ggagaattct taattagaga tttgcctaat ctagagcaaa
     1081 cttggataga tgctctggaa tcagcaggca ttcgcttgat agagcagttc ctggaattgg
     1141 atgaagaaga gattgcacgg aacactgctc tgagcgctac agacatcagc gcaattcgtg
     1201 acataatcga agataatgtg gaaatcatac aggaagaggt agaagaagaa ggaatcgtgt
     1261 cttatagttg tccggaatgt ggatcaccaa taactataga catggatcat tgcccatcct
     1321 gtggagtagc cctgagcttt gaagcagaag aagagcagga tgaataaagg aagaatatga
     1381 cagagagcaa cgataaagac cttaagaagg tgcatttaat caaacaacct aggcaagata
     1441 gtcccgagca gcctcaggaa atgtcggctc agaatcctct cgatcagggc gcagaaaaaa
     1501 agaaggttgt tgtggtggta aagaaaaaaa caatcaccac aaagaaagtt caggcaaaag
     1561 caatgatccc aggaactcct aaagatcaaa aaatcgatga agaacaatcc gcattgtcaa
     1621 cacagccgca ttccgaagta ggacaaaata aagcgcagat agcaaaagag gatcaagtcg
     1681 catctccttc tccgataacg ccctcgacaa cgacgcttcc atcatccaaa tcaccatcaa
     1741 atgttccatc aatgaaattg tccgaacaca agcaggaaat agggcaagat gagacaatta
     1801 tcagctctca gcggcggtac tcttcggatg ggcgcgcagg aaatcttgct caaggcagac
     1861 ctagttctac atttcggaat agtcatgata gaggaagttc gcgtcctgct aacggatctt
     1921 accaaggcag agcatatggt cagccatctg gaaggagcta tccatcatca tccagccgat
     1981 ccggacaatc cggatctcag cagttttctc gtggaaatcg tccttctggt ggctttcaga
     2041 gtcaaggtgg aacaggctct ggtggtagga cagcatatcc ttatgcaaaa acaggtcagc
     2101 ccggaagcgg ccaaaggccg ggtggatata ttggagcaag acagggccct gcaggcggca
     2161 gatacggatt acgtcaaact cccgcaccgg ttccttccac tgggaacgaa aggcccgctt
     2221 cgcgtagagc accggtcaaa aagaaaggag tattcaacaa gcgcgaggaa gaaacggagc
     2281 tcgagaagca aattcagctc aaaaaaaagg ccgaagccaa actggcagct gttccgaaat
     2341 caattgacat catggagaat atcagcattt cagagctggc aaagaaaatg aatctaaaac
     2401 ctgctgacct gataagtaaa ttgatgtcac ttggcgttat ggcgactatc aatcaaaaaa
     2461 tagacgccga tacagccgca attctagctg ctgaatatag ctgcgaagtc aaagtagtaa
     2521 gcctctatga tgagacagta atcgaaaaag cagtggatag accagaagaa ctcaggccaa
     2581 gacctccaat cgtgactgta atgggacatg tcgaccatgg aaaaaccaaa ctcctggatg
     2641 cgattcgcaa aacggatgta gtgtctcaag aatttggtgg tattactcag cacatcggtg
     2701 catatatggc tgaataccct aatggaaaga tatcttttct tgatactcca ggccatgctg
     2761 cattcacaaa aatgcgagct cgaggagcaa atatcacaga tatcgtagta ctagtagttt
     2821 cagctacaga aggcgtcatg atgcagacca aggaggcgat agatcatgca aaggctgcaa
     2881 atgttccaat tattgttgcg attaacaaaa tcgatctgcc ggaagccaac ccagacagag
     2941 taaaaacgca gctctctgaa ttgggcctta ttcctgaaga atggggaggt cagacacagt
     3001 tcgtcgaagt ctccgctctc caaaagatag gcattaggga actctttgat gcaattctcc
     3061 tgcaagctga aatgctcgac ttaaaagcaa attacgacag gctcgctgaa ggcaagatca
     3121 ttgaatcccg tatagaccaa ggccgtggaa ttgtcgccac acttatggta caaaatggaa
     3181 ctttgcatgt cggggatcct tttgttgcgg gtattttccc aggtcgagtc agagccatgt
     3241 tcgatgataa aggaaatcgc atagaacagg ctacgccttc gatgccagta gaggtaatcg
     3301 ggttcgaggg catgcccgaa gctggcgatc cattcgaagt ggtggaagat gagaaatttg
     3361 cgcgtcaaat ctcaaccaag cgccaagaat tgaagaaata cgaagaaggc cgcaatgtca
     3421 aaaaagtgac tctggataat ctctacgaga ccataagcca gggagagatc aaagagctga
     3481 aggtcataat aaagggagat gttcatggta gcgtagaagc tctaaaaggc atgcttgaaa
     3541 agctatcaac aaaagaagtt catttaaatg ttataagagc ggcggcaggc gctatcacag
     3601 aagatgatgt catgatggcc tcggcatcca atgccatcat cgtcggattt aatgtccgcc
     3661 cgacatccaa tgcaaagcag ctagctgagc gcgaaaaggt cgatattcgc aagtacaatc
     3721 ttatatatcg cgcccaagaa gagataaaac gcgcaatgga aggcttgtta gctcctgaac
     3781 tcaaagaaca agaagtcgga aaagctgaag tacgaaatat cttcagagtg ccaaaagctg
     3841 gcatcgttgc aggttgtttt gtttcagagg gtatagtcaa gcgaaattgc caagtcagag
     3901 ttatccgcga caatatcgag atattccaag gcaatctatc ttcccttaag cgattcaagg
     3961 atgatgtcaa agaagtagcc gcaggttacg aatgtggtat cggcatagag aactgcaatg
     4021 atcttaaaga aggcgatgtc cttgaatttt atgagacaat tgaggttgct cgaacgctcg
     4081 aatcgggaga taaaaatgaa ccaggtgcgc aagcgtcgtc ttgaagaatt aattcgagag
     4141 gaaatttcta agatgatcac ctttggtgag attaaagatc ctcgtgtcaa tagtttttta
     4201 tcaatcacaa gggttgaggc aagccaggat ggatcgcatg cgaaagtatt tgtaagtagt
     4261 cttgatgata tagaggggca tcttgacgaa gcaatcgtcg gcctatctca tgcggctggt
     4321 tttgttcaat ctctcatcgc aaaacgcgtt cgtctgcgat tgactcctgt tctcaccttt
     4381 gtggctgatc acagcctcaa agaaggattc gagcttactg aaaaactgaa ggatcttctg
     4441 aaataatgca gcatagcggt tttttcctcg tggataaacc taaaggcttc acttcgaata
     4501 gagtattgca ggacattcga aaaagcttat cggttcaatc ctctgcgtat cctcagaagt
     4561 atgggcatgc tggtacgctg gattcttttg cttcaggttt attgatcata ctcactggca
     4621 agataacacg tcttagtcga tggttcatgc atcagaacaa ggaatatgaa gcagttttca
     4681 gattcggaga agagacagat actctcgatc ctcaaggcaa aatcatggcc ataggtgaat
     4741 tgcccgcgag agaaaccctc gaaagaatta ttccttcttt ttgtggaaat attgaacaga
     4801 tacctccatc atatagtgct gtgcatgtcg gcggggaaag atcatataaa atagccagag
     4861 ccggtaataa acccgaaata tcgccgagat ccataataat acatgatata gagttagaga
     4921 gtttccaaga gaaagaagcg actcttagaa ttcgctgctc ttctggcact tatattcgtt
     4981 ctcttgcaag agacatcggc catgcatgcg gctcatgtgc aacggtgata tcgttgagaa
     5041 ggacagtaat tggacctttc tccataagcg aagcacattc tccgcaagag tgcaatgaga
     5101 agagtatcag agtcttctca ccggatatcg cgcagagctt aggtcttgca acagcagtaa
     5161 tcaaattgga ttttgttgag cattttattc atggagggac attgccagct aaagccgtaa
     5221 cattagattg tgaccagatg acattattta gaaacccaga cttttccttt gaatcaaaac
     5281 cagtggcgct tttttccgaa aaaggggagc ttctgggtat agcagagcaa aaggataaag
     5341 aatacgcgcc gagagtggta atggcagagg agcctcgata gtgaaagttc tgacctggca
     5401 ggaatttcaa aataatcact ctcaggaaaa gcttgcagct acagtcggcg tctttgacgg
     5461 actccattat gggcaccaag tactgataga tactataaag ttacagagcc ccgccctgaa
     5521 gccgtgtatt atcactttta aggaaaatcc caagaaaata ctcaatccat cgagctataa
     5581 aggtagcctc ttgactctag agcagaaatt atcaggtttt cagcatatgg gtatagaata
     5641 ttgtgtgatg attgactttt ctgataattt cgctaaactc tcgggaaggg aattccttgc
     5701 tgcactgtat attgctaatg tacagtttat agcggtgggt gtgaattttc agtttgggca
     5761 caacctcgat accaatgctg ccaaagttga gaagctgagt gaagagattg gcttgcgttc
     5821 atgtattgta gagaatgtca tgtaccgagg ccacgcaata agctctagca gaattcgcca
     5881 tgccgtgctc gagggaaggc ttggagaagc cgcagaaatg attggtaggc catatcaagt
     5941 actagccaga cgggagacaa gcgttataga cgaaaaagca gaattctttt tgcccgaaga
     6001 tgatttgctt ataccaccgg aagggcagta tgaggtgttc ataaaggata acgatacggt
     6061 gcagaagacc aaagtgaacg ttgttcacaa ttatatagca ttgagtttac gcatgcgatc
     6121 ggataatatc cggttggcgt tcgttgataa ggctgttcaa cagaaggaga atgtagtatg
     6181 gctttgagca aagaagacaa ggcccagtta gtgctcgagt atggcaagga ttcaaagaat
     6241 acaggtgcga ttgagaccca ggttgcaatt atatctgcga ggatcgcata tctgaccgag
     6301 cattttaaga ctcataagaa agacacgaat tcaagaaggg ggcttcttaa gcttgtcggt
     6361 cagcgtcgaa agctcttaaa atacctacag agaaccgatc ttgaatctta tcgtgcaatt
     6421 atggaaaaat tgcagatccg aaaataataa tccaatccga gcaatattaa ttgatccgga
     6481 ttaaatcgga tatgcgaatc tagagaagga atgtaatgat aaacaaactt gaatgtaaaa
     6541 ttggcaacga gaatcttgta tttgaaacag gaagaatggc aaaacaggca aatggagctg
     6601 tttttgcgac ttatggtggt agtgctgtta tagcaactgt ttgctgttcc cccacgccaa
     6661 tagaaggttt ggattttgtt ccgctgactg ttgagtattc tgagaaatac tatgcggcag
     6721 gcaagatacc aggcggtttc ataaaaagag aaacaaagcc caaagataga gaaatcctcg
     6781 tttccaggat aatcgatcgg cctatgaggc cattatttca taaggaattt ggacgagaaa
     6841 ttcagattgt gccaacctgc atctctgccg accagataaa tccgcctgat gttatagcgg
     6901 caaacgcagc aagcgccgca atccatatct ccgatatacc ctttgatggg cctgtgggat
     6961 gtgtacgtgt ggcgcttgtt gaaggttcct atattcttaa ccccacttat gaacagatag
     7021 accatgccaa gctggaaatc gtagttgccg gtacagctca aggtataact atggtggaag
     7081 gtggatctca tgaagcttcg gaagaagaaa tgattcaggc tatcgagatg gcaagagaac
     7141 ctatttcgca aatctgttcg accatagaag agctgcgtcg gattgccggt aaagaaaaac
     7201 tagcaccagc accattttcc atagagttga acaataaaga tgaaattcgc aaatgcgctt
     7261 atgaattatt atcaagagct cttttcacga aaataaagca agagcgtgca aaagccgtat
     7321 cagaggcaat atctgccgtc aaggaaaaat tcgcggatga tcttgacgaa gaaattcaga
     7381 tgaagctttt ttcaaaattg atggatgatc tgcagtacga aattctacgg tcctcgatac
     7441 ttgacaaagg gttgcgtgtc gatggtcgcg gtctggagga aataaggcca ataacttgcg
     7501 aaatcggcgt attgccacga acccatggtt ctgcattgtt tactcgtgga gagacccaag
     7561 tgctcgcggt gacaactctt ggcactgtat tcgatgagca gatattcgat gatatcgaag
     7621 gagatcgacg cgagcgattt atgcttcatt acaattttcc tccctttgcc gtgggagaag
     7681 ttggcaggct tggaaccgga aggcgggaga ttggacatgg cgatctagct cgcagatcta
     7741 tcgaaccgat gttgccgcca aaggagagat ttccatatac tattcgcgtt gtggcagaag
     7801 tgctcgaatc caatggttct tcctctatgg ccacggtgtg ttcttcatcc atgtcgctta
     7861 tgcatgctgg tgtgccattt accaaacctg ttgcaggtat agccatgggt ctagttactg
     7921 atgatacgcg ctatgctgta ctttcggata tcctgggtga cgaagatcat cttggtgata
     7981 tggatttcaa agtagcaggc acaaaagatg gtatcaccgg ctttcaaatg gatatcaaga
     8041 tatcaagcgt ttcgacagag attcttcgca aggctctaga ccaggccaaa tgcggccgac
     8101 ttcacattct ctctattatg gaaaagacta tcgctgaacc acaaagcgaa atatctccct
     8161 atgcccctca ggtgattaac gcgcgaatcg atcccgaaaa aatcggcctc gtgatcggcc
     8221 ctgcaggcaa gaatatcaag gcactctctg agaaatacga tgttcagatc aatatcgatg
     8281 aagatgggta ccttacagtc tacggcaaag accagaaatc tacctatagt gctcgcgatg
     8341 ccatcttggg aatggtagag gaaccggaag tcggaaagat ttataacggc acggtaaagc
     8401 ggatcatgga tttcggcgct tttatagaaa tacttccggg tcgcgaggga ttatgtcata
     8461 tttcgaaact ctcaaaggaa catgtagaca aggttagcga tctgcttaaa gaaggcgata
     8521 tcataaaggt taagcttatg gaaatcgatc atcttggaag gctcaatctt gctgcagtgg
     8581 atgcgcttga tgacaaggga cagatagcgc agcgagatgc tagacggaat gacaggcctt
     8641 ctgatcgcca gagagacatg aggtcatctc gccgggattc gcattgggat aggtaatcct
     8701 cgatcgatat gatataattt aatatcaatt cgctctttgt agtcgatctc tatggatcgg
     8761 atatatcatg agatgatctt gatttagtga ttaaggatat tttgacatgg tgaagcccga
     8821 agatagagcg atagaagtac taatagcgct ctccgaagga gcacgaattc ctcaatatgc
     8881 tacagataat gctgcaggag cggatctcca tgctgttctt cagaagcctc tggtgctcga
     8941 acctctctca agggcattga tatcgacggg tcttagaatt gctttacctt cgggctatga
     9001 agctcaaatc aggcctagat caggctgggc gtataggcat ggtgtcacct gcctaaacgc
     9061 tcctggcact attgatagcg attatcgtgg agaaatcaag gttattctca tcaacctgag
     9121 tgacaaaccg tttgtcatcg agaatggtga tcgaattgca cagttggtca ttgctcctgt
     9181 tgttcgcgct gatttctcga tttcctctca tctcgatgat actactcgtg gggctggcgg
     9241 ttttggatca actggccgct gaggataaga ctgagatgtg agctgagatg aaagagcgac
     9301 cctccttcac actctggcga ataacgattt cagagataat gacttctttc ttgggcacat
     9361 ttctattctt ctttattgta tttcttatca atcaagtctt gctctttgcc gaggatatcc
     9421 tatccagggg tgctgatctt ctatcggttg tcaagctact tttttattct ctacctacta
     9481 tactcgcgat cactattcct ttttccattt tagcggccac tctcatgaca tcatcacgac
     9541 agaattccga taacgagctt ctggcaagtt cttccttggg tataagacct ctttggttat
     9601 atgttccttt tctgataatt ggattagcca tttctattgg atctttttgc ttcaatgaat
     9661 ggtctatacc ccgtgccgct caaggcttca agaatgaata tgcagagctt attaagaaat
     9721 ctgctaaaat tgagctagct ccttattcaa tcagtaaata tggagataaa ctgcttgtca
     9781 ccggtggatc ggaggagggt tcttccacaa gtcgattaca agatgtacta attatcgatc
     9841 agtctggagg gtatgattct gataccgtga tagcggatga tgtgggaata gaattctcta
     9901 aagattctct gatggctatt ctaagcatga atagtcttac agaactaaag agactaaatg
     9961 atgcctctgc gggagatttt tctgtgacta tggcaaagtc cgccgaaatt cgaattagga
    10021 tccgcgaacc cttaatgaat tatagtagta cggctccttc tgagatgtct ttgacaatat
    10081 tggcaaaaaa aataaaagaa aaagagcgga ttcttgatga tcggattaaa aaaaaggatt
    10141 ttcaaaagga tacagcgctt gatagattaa ggctgaatta tgatttgctt gccattgaga
    10201 ctcagcaaga gagatcattg aagacaaaag gctcactgac ctcactgacc ttgttacaaa
    10261 atgatctgat atctcttacc aacaagataa agtcacttga gaatcaaaaa atcaacgata
    10321 catcacttca gatttatcgc cttgaatatg agaaaaaatt ggtcctgcct tccgcttgct
    10381 ttttctttgc acttttagca ctcccattag gaattggatc aaaaagagca gggcgcgcag
    10441 ccggttttgg tattgcactg cttctttcag taatctattg ggcattgtta ttcctaggtc
    10501 agactctggg ttaccgccaa aatgtcaatc ccgtgttttc tatgtggatg ccgaatcttg
    10561 tcatgttgct tgccacaatt tctctatggg ttgtcagaaa aataaaaacc gggcattttt
    10621 tatgatgcct aaaaaggaag tggaatcgaa catggctcaa caatcatcag cttatcaaca
    10681 agacactcga tttttgccga ttattctctg gagagctctg ttgggaaaag tatttatctg
    10741 gactattgga ggaactctat ttctttcact tgtaatttct cttgtagaac ttttttctct
    10801 tttatggaaa tttcttgctc aaaatgctct tttttgggat atcatctttt ggattagcct
    10861 gggaattact gatcatattg tcgatgcttt gccggtagct tttctttttg caatagtctt
    10921 cgttttgagt gattggcatg caaaccatga acttgaagct gtctttagtg caggattatc
    10981 actgcaacgc ttgttgattc ctattctgag tatttcgata ttgttttgtg ctgctgagtt
    11041 ttacttgaca gactttgcat ccattccatt ttctagatcg agaaatgatc tgcagtctag
    11101 aatactcaaa gaatccgcag gaaaacaaac agtgcctggg ctgatcgttg attcaggaaa
    11161 acaggtctat acttacagat atttcgatga aaagaatctt cgcctatatg atgtttctgt
    11221 aatcgaaaga gatgcctatg gaaacctaac aagaaaaatc tcggcagaaa gtgcgaaatg
    11281 ggagcaagga acatgggttt ttgcaaattc gataatatat agcaggcaag acaacgactg
    11341 gacattctca aaattcgaag agtattccga accagtgata aatgaacctc cttcgagctt
    11401 cacaagacca agcatgaatc ctcgttttct gaccatgaga gagctcaaca atcatatcga
    11461 attcttaaag gcttccgggc ttcccgttgt ggatgcagag gttgaaaagc aaaaaaggct
    11521 atctttttcc ttgactcctc tgattgtggt tggtcttgca gccgctttct caggccgttt
    11581 caaaaagagc gttttcttac tgagcttgct tcttagcctt tgtagcgcaa ctctatacta
    11641 tgttgcgcag atgatagctt ccgtatctgc tagatcaggc ttgatcagcc cgagaatatc
    11701 catgtgggcg gtaattattg tctttggtgc tttatcaaca atctcttatt tgagtgcaag
    11761 aacttaaata tgtattattt tgaataatat tggagtagcc ctatagatga ttaaatcgat
    11821 ctttcaagag aagcacagag atccttcatg ttctgcgaga accggtttaa tatcgcttcc
    11881 gcatggagat gttcagactc ctgctttcat gcccgtaggt acaaatgcca cggtcaaggc
    11941 agtggaacca agggatctag agtcgatggg attttctttg attctttcta atacatatca
    12001 tcttttcctg aggccaggtc cggaagtcat atccaaggct ggcggtttgc atggtttctc
    12061 tggctggcaa agaaatttcc ttacggattc tggtgggttt cagatatttt cactgtctca
    12121 attcagaaag attctagatg atggagtgcg ctttagatcg catatcgatg gttcttctca
    12181 cattctaact ccagagaatg tggtcgatat tcagactgca ttcaattccg atatacaaat
    12241 ggcattggat atttgtgcgc cttggggtga aagcgagaat aaggcatata aagcaacagc
    12301 cttgacttat gattgggcaa agcgagccaa aactcagtgg ctttgtcaga gagagcgtgg
    12361 gtatcaaggt tatctttttg gtatcgttca gggcaatttt tacagagagc ttagaaaaat
    12421 gagtgtggaa caaatcgctt ccttggattt gccaggtatt gcgatagggg gactttccat
    12481 cggagaaccg aaagaagcat atatggaata ccttgacttt actgcggctc ttttgccctc
    12541 agataaaccc cgatatatta tgggcattgg aacaccagat tacatcatag aggcagtgcg
    12601 gaatggtata gatatattcg actgcgttta tcctaccaga acagctcgga atggtttatt
    12661 attcacttct aggggccaaa ttgtaatcaa gaaagctata tataaaaaag attttaggcc
    12721 tatagatacc gattgtagtt gccgagtgtg caagacatat agccgtgctt atctgcacca
    12781 tctattcagg aacaacgaaa ttctatactc aatgcttgca agtcaacaca acctgcattt
    12841 tatggcagat tttgcttcga agattcgtca agcaatccaa gaagatggct ttgaatcatt
    12901 tgctcgagcc tttcttgatc gctatactgg catggaagag aaagaagaag cgcagtaata
    12961 acaagaaacc ctaaaaatcc gctttttcca cctttacgac ttcataactg aatttacgct
    13021 cattgattgt gaagttcagc ttttcgcccg tcttatgatt gagcaagtgc ttgcctagtg
    13081 gagaaagata agaaattacc tggtgctcag gatcggattc ccatggtccg agaatagtaa
    13141 agaattcatc ttcgccggtg attagattct tgagcgcaat ttttgtacca aaagaaatac
    13201 tggtgctgtc tacctgatcc gaatcgatga tctgggctcg atctagttcg cttttgagtt
    13261 tgcccacttt ggcgttgagt tcgtcttgcc tttcctttgc agctttgtat tctgcatttt
    13321 ctcgcaaatc gcccagtgag agggcatagg ctatttcttt ctggtttgca ggaacatcta
    13381 cttccattat a
//
LOCUS       DAOOWY010000051        13020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4804_length_13020_cov_9.589510, whole genome shotgun sequence.
ACCESSION   DAOOWY010000051 DAOOWY010000000
VERSION     DAOOWY010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4804_length_13020_cov_9.589510"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PK105_05300"
     CDS             <1..1285
                     /locus_tag="PK105_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61160.1"
                     /translation="VNSCAQAFDRVGLPEGQLHLVHATLYCALAPKSNSILCYFDALE
                     AVQSEQAEVPDHLKDANRDKVAFGHGEGYQYPHAYREHWVAQQYLPFSLMGRLFYIPG
                     ELGYEGTKRSDVLIRREAQLAIQPEDLNLIEDVNLIWSKKGKSRLSWRIRSESSTTDR
                     LLAIRTAFFNHLPLKPFDNIIVVDPRSGFYTMEALRRTREGKAFVLLGNKEAKMELEN
                     LSKTEEEIIQPEILTYGNFTTSGATSAALESFFSEAKPSFIILCEFCRVPIDVSEFLA
                     GLAGNVENGLLSFLAFDIDVETSSRLSSALLAMENLSNKEKEFCERLLAFENELGHGP
                     TYARLDSEKCSKQEWFEKKSSMGSSGFRKAILRASYSRPIDEKELERWLDSSTFYGSN
                     FQKAFSESETVMLKSLVSKFNRDPQWPLEILCFEF"
     gene            complement(1268..2290)
                     /locus_tag="PK105_05305"
     CDS             complement(1268..2290)
                     /locus_tag="PK105_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61161.1"
                     /translation="MRNIHAAEERQPAKSPIHRRGRGWGSSQKALSQSYESTKIDQPL
                     HLHDKQVVVIIESEGAVFNIHKYWHEMAYLPSFIGCFGGNVDPSIVGEVWRTVALETS
                     LRGENPIVILLAALRVLNALFPSMQRAVIIKTLEKYSLSEKTLEDLFNLNEKNPAEMM
                     IIDWFSMAEGLIQEIGRVPHFSTAEEFLHSVKYVAPRSEVLVYSRLAELSAMRMWQNA
                     GLGECFYRLAGRERGKPGDYLRAALSAGFERSSMVAIGASAAMFRAAQLAGIRFYPIT
                     PGLEEDAWKTLAEHWFPAYLRGEAWKVDMHAEPFYEQMCQEFNVMKTSRYIATRLHLT
                     SSKFEA"
     gene            2410..3783
                     /locus_tag="PK105_05310"
     CDS             2410..3783
                     /locus_tag="PK105_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOU61162.1"
                     /translation="MSNRHFSSIDGVYDFVLGFVNVEKGQATEFKLDRMHWMAAQLGN
                     PHLGRLTVHVAGSKGKGSVATLIAKTLESSGLRTGLYTSPHILSWKERITESGIEMAD
                     ELLLKAAEEVYSLVEGRTQEDFPGNELPTFFELTTLIGFCAFRLAGFEAQVIEVGLGG
                     RLDSTNIVSPNVCVITPIELEHTQFLGSTIPLIAAEKAGIIKRSVPVCTIQPKTEAFR
                     VISKKAEDIGAPCLLVGRDISLSDIKINIHGTCCRLSATDTTPSSLLRLMRGASIEIS
                     TPLVGSIYAGNMAVSALALSQLDIELNTEHMLQGFSSSSMPARFEIVSHSPFVVLDGA
                     HTPESIRTNLSTFLELSKAPRILLFGCAHDKKHDEIAKIISPYFDEIIITRPGTFKQS
                     EPAIVFDSFQKQMSNSIISDRYLIEDTNSAIAKALSDAREKNASLLVTGSFYLCAEFK
                     KSRLCSE"
     gene            complement(3755..5437)
                     /locus_tag="PK105_05315"
     CDS             complement(3755..5437)
                     /locus_tag="PK105_05315"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOU61163.1"
                     /translation="MEFFKAWILPPLVGAIIGYFTNWLAIKMLFRPYKTIKFAGIALP
                     FTPGLLPREKDKLAVSLGETVSKELLTAEVITQRIQSADIKQTILKAIKSALASFFNQ
                     DAGRIFSQIGEPGLEEKKDDEPSLLLLESNGQKITKEVQSGNGTTIDSEVSALQRNII
                     EALQHLVERKSVQSSIEGIFAEFLNRIGKLSFKELVSKEQFIASVLKACSTLQTRVLS
                     ESNHEQSLQSSKSKSSRFNPLLKAVIDLPPDVTIMAFTDALVPQVYTWFLDYIDSFFH
                     SEGFRARLETEAQAFVKRALDRLGPIQRLFISIAGYDAKIAQAMPENIEDLIQTIERI
                     LKDPSTPQSISKTISRAIINWRSKDEAETTKTGPEIYQRADDSIGSKSRVVSEQIVLS
                     LKDSSEDLRLRAEAIYHSLADLEFGEILGIRSNEEAISGFVFSIIVPTMHVSDSSASN
                     TMGSIFIEVLKDVSKGKTFADILKIQDSEIERISVSLTDGLIELIQSRISTLIDAFDI
                     TSMVSERIDSLDMKEVERIVLQVVKKELAWITWLGGILGGMIGFIQSIISTF"
     gene            complement(5440..5661)
                     /locus_tag="PK105_05320"
     CDS             complement(5440..5661)
                     /locus_tag="PK105_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU61164.1"
                     /translation="MKKDPTNSSQNLSILRKGTVLRIIESTYSTSESDRGTLWFRIQE
                     AGQTGWVPALEVMTYSSEKQARNAALRME"
     gene            5872..6525
                     /locus_tag="PK105_05325"
     CDS             5872..6525
                     /locus_tag="PK105_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61165.1"
                     /translation="MNMTIGKAKAKPFLFLVFMVCKLGATQAIGALPALEFGMGFAYA
                     RTKVESAATGYHHGWNVSADANWLFRISLEDENLIKAKAEKETSSRVNMIFSPGLHAQ
                     FITMSASNWLSDGSRYRAWDSIGLGPELNLGLEFTNSMTMHKNGFLFSMAYLASFSNY
                     TQTTLYSAYNSWFLKVSWNARIDNGAAFFAALPLEYAFRADGNSLLSGLVIGMRYEF"
     gene            6515..7552
                     /locus_tag="PK105_05330"
     CDS             6515..7552
                     /locus_tag="PK105_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU61166.1"
                     /translation="MNSKTLHTSSKYTYMFMCIKMKDFAKETSKYGGLMKGAFFLLVL
                     WPLLASCEILAPTNATFEGEKYAVVVGINDYIDNTNNLIRDLDFCAADAEAMKDMLTG
                     AGWQVELITAEANESIKKNATKEKIKTAFQKDKDGLTAFLFYFSGHGDVHEDQAYIVP
                     SDAYRSGNSVEKSSMISATELSMWLKDLNANKIMIFDSCYSGGFVNPGDCIDAAPGNI
                     ESDQISTNIEMFFRFGEILAQNSASAARSPSTAPLVISASGSNEASMEDANERPTHGS
                     GHGFFTYYFLQSAEASKDGSMKGDLDGDGILSCLEAYNYAKNALEKDWIASQLPHITG
                     GLRDFALIDLR"
     gene            complement(7594..8082)
                     /locus_tag="PK105_05335"
     CDS             complement(7594..8082)
                     /locus_tag="PK105_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOU61167.1"
                     /translation="MRYNLTTAYFLWLISGFGALGFHRFYLGKTGTGLLWMFTGGLGG
                     LGGLYDLITLPRQVREANLAYSAREAIGHENYGQWGEFIPHIRQSETPERVILRLAKA
                     NNGMVTAGEVAIEANIPIEEAQRQLDNLAKKSIAQVRVRSSGVLVYFFPEFSKEDTDF
                     ID"
     gene            8215..9615
                     /gene="asnS"
                     /locus_tag="PK105_05340"
     CDS             8215..9615
                     /gene="asnS"
                     /locus_tag="PK105_05340"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOU61168.1"
                     /translation="MTLLPTIKQLLSMEAVGQPVIIRGWVRTKRETKQAIFIEVNDGS
                     CLSNIQCVLDSSLEKNQSIAETILRLSTGASVEIHGELVPSPAKGQKVEVKCQQIKLI
                     GEAPSERYPLQKKAHSLEFLREIAHLRVRTNTFAAVARVRNRMAFAIHQFFQERGFYY
                     IHTPIITTSDAEGAGAMFQVTSLDIEELSKSGTLIDYDKDFFGRKAFLTVSGQLNVET
                     YCQALSRVYTFGPTFRAENSNTTRHLAEFWMIEPEMAFAHLDDNIALATEFIQYLVAT
                     AIRDCAEDVAFFDQRIQPGLRESLERIVQSSFARMSYTEAIRELEASNANFEFKPYWG
                     CDLQSEHEKFLTEKVAGGPLVVTDYPKEIKAFYMKQNEDGKTVAAMDVLVPRFGEIIG
                     GSEREWRLDLLEKRMEELGLDKEPYQWYLDLRRYGSTPHSGFGLGFERLLLYITGMTN
                     IRDVIPFPRAPRQADF"
     gene            9633..10925
                     /locus_tag="PK105_05345"
     CDS             9633..10925
                     /locus_tag="PK105_05345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOU61169.1"
                     /translation="MKLLTAIKRHQTCINKASLLIIAIISCFFIFDFPAAANSIESFS
                     RLGVEIPNPPSLNARSAILIDAATGTVLFEKEADIRLPPASLTKLVTLHIVMEEIKSG
                     RLSPDEIVEIDPQDCSPSIPYGSSLMYLQPGMKVSVKDLMLGAAVVSGNDAAYSLARR
                     IAGSNEEFAEMMNKEVKAMGFQSLCFVEPSGLSEFNLVTARDFAFFCKKYIELHPKAL
                     KELHSVHSIQFPRPEHATSQYRPNGIVLQYNRNPLIFSYEGADGLKTGYIIEVGYNMA
                     ATAMRNGNRYIAVLLGGSSTPYADGAALRTKDTKALFDWAFANFATARPGYIMPKPLR
                     VWFGKSIWIDPEPEGEAAATVPVVYKDRVTARIETVKGIKAPVYKGQKLGEIVYEIDG
                     TKINSVNLVSGQDIPRGNLFRTLLDSIQYFFAYIFGKL"
     gene            complement(10998..12032)
                     /locus_tag="PK105_05350"
     CDS             complement(10998..12032)
                     /locus_tag="PK105_05350"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOU61170.1"
                     /translation="MKNVVAALFIISILSFPFSAFGEITLKLAHLNTQAPFEVVSAAM
                     SAVFKSEVESKSDGQIKVDIYPDGVTGKEIETLIQVKSGQVQSSISTSGAIAQFYPLI
                     DVTNIPFAFSSYNVAYHVYDGQFGKELAADIEEKAGFHVLGFAESGGFFAITNSKRPI
                     RSPGDLKGLKVRTMAIPLHQEIIRALGATPVTIAWSELYASLLAGVVDGQMNPVSIIV
                     MDRLYEVQRYATLCNLIYSPYVWVINMEFYNSLSKELRIVVDDAARTAIVAGRGLSRI
                     IDSTERGLPMLSGKMQVYVPSGAVMKQFKDIVVPAANKFLLTQYKEEGSIWMNKFVAA
                     IDKAEKELGY"
     gene            complement(12043..12453)
                     /locus_tag="PK105_05355"
     CDS             complement(12043..12453)
                     /locus_tag="PK105_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOU61171.1"
                     /translation="MFQVPTKTQYAIRALVHLVHVKSASVASIAEAECIPSKYLEAIF
                     GKLKNAGLLIAERGRNGGYRLAKDPALVRMSEVVRATEGEIRIVECVDNAAICLLGQG
                     CLPRHFWLGLKDAVDLYLESVTLAEIANPDMSSA"
     gene            complement(12513..>13020)
                     /locus_tag="PK105_05360"
     CDS             complement(12513..>13020)
                     /locus_tag="PK105_05360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOU61172.1"
                     /translation="CHEAGVTEPVERFMEPMIVDVRSDAESGNYYFYQAATGQIVFCI
                     TPRPQIWGRDKDSTSTFLPLVVRRMLELYPRLRNLRVRRTWRGMYPMTPDGLPIIGYP
                     DLAVNFLQLVGMCGQGFMMGPGLGSIIARSIAANGCHERPEGFTDYGFLFEELSINRS
                     FDHAELLS"
BASE COUNT         3688 a         2981 c         2801 g         3550 t
ORIGIN      
        1 ggttaattcc tgcgcccaag cctttgatcg tgtgggcttg cctgaaggtc agctccatct
       61 tgtgcatgca acactctact gtgcacttgc tccaaaatcc aactccattc tttgctactt
      121 cgatgcctta gaagctgtgc aatcagaaca ggccgaggtt ccagaccatt taaaagatgc
      181 aaatcgcgat aaagttgcat tcggccacgg agaaggctat caatatcctc atgcatatcg
      241 agaacactgg gtagcccaac aatatctgcc tttctccttg atgggaagac ttttctatat
      301 tccaggcgag cttggatatg aaggtacaaa gcgcagcgat gtcttgatcc gaagagaagc
      361 gcaactggca atacagcccg aagatctaaa cttgatcgaa gatgtgaacc ttatatggtc
      421 taaaaaagga aaatctcgac ttagctggag gattcgcagt gaatcttcga caaccgatcg
      481 cctccttgct atacgcacgg cttttttcaa tcatcttccg ctcaagccat ttgataatat
      541 tatagttgtc gatccacgat ccggattcta taccatggaa gcgctgagaa ggactcgcga
      601 aggtaaggct tttgttctgc tcggcaataa agaagccaag atggagcttg aaaacttgag
      661 caagacagaa gaagaaataa tccagcctga aattcttaca tatggaaatt ttacaacatc
      721 tggtgctaca agtgctgcac ttgaaagctt cttttcggag gctaagccat cttttattat
      781 cttatgtgaa ttctgccgtg tccccatcga tgtaagcgag tttttggctg gccttgcggg
      841 aaatgttgaa aatggattat taagcttttt agctttcgat atagatgttg aaacatcgtc
      901 gcgactttcc agcgcactac tggctatgga gaatcttagc aacaaagaaa aggagttttg
      961 cgaacgtctc ttggcttttg aaaatgaact tggacatggt ccaacctatg caagacttga
     1021 ctcggaaaaa tgttctaaac aggaatggtt cgagaagaag tcgagcatgg gctcttccgg
     1081 ttttcgaaag gccatactca gagcttccta ttctcggcct atagatgaaa aggaattgga
     1141 gagatggctg gattcctcga ctttctatgg ttccaatttc caaaaagcct tctccgagag
     1201 cgaaacagtg atgctcaaga gtctcgtaag taaattcaac agagatccac aatggccttt
     1261 agaaattcta tgcttcgaat tttgatgatg tcaaatgcaa acgtgtagct atgtaccttg
     1321 aagtcttcat gacattgaat tcttgacaca tctgctcgta gaatggctca gcatgcatat
     1381 cgactttcca tgcttctccg cgaagatagg ccggaaacca atgttcagcc aatgtcttcc
     1441 aggcatcttc ttccaggcct ggagtgattg gataaaagcg aatgcctgca agttgagctg
     1501 cgcgaaacat agctgccgat gctccgatag ctaccatgct cgaacgctcg aaacctgcag
     1561 atagagcggc tcggagataa tctccaggct ttcctctttc tctccctgca aggcgataga
     1621 agcattctcc aagtccagca ttttgccaca ttctcatcgc cgaaagctcg gcaagacggc
     1681 tatatacaag cacctctgaa cgaggagcaa catactttac gctatgtaag aattcttccg
     1741 ctgtgctgaa atgaggtact cttccgatct cttgaatcaa gccttcggcc atagaaaacc
     1801 aatcgatgat catcatctcc gcaggatttt tttcgttcag attaaaaaga tcttcaagtg
     1861 ttttctcgga gagactatat ttttccagag tcttgatgat cacagctctc tgcatggaag
     1921 ggaatagcgc atttagcacc cgcaaagccg cgagaagaat aacaatcgga ttttctcccc
     1981 gaagagatgt ctcaagagct acagtcctcc agacctcacc cacaatcgat ggatcaacat
     2041 tgccaccaaa gcaaccaata aaagacggca gataagccat ttcgtgccaa tatttatgaa
     2101 tattaaaaac ggctccttca gattcgataa taaccactac ctgcttgtca tgcagatgca
     2161 gaggttggtc tatttttgta gattcatagg attgagaaag tgctttctgc gaagatcccc
     2221 aaccgcggcc ccttcggtgt atcggggatt ttgctggctg cctttcttct gcagcgtgaa
     2281 tatttctcac atcatcctca tctaagctta tcggcaagtt gaagctagcc ttttatcaaa
     2341 gcacaaatct attttaaaca atagacaatc tcccttgacg aatttaagcg cattgcgcat
     2401 gctctatccg tgagcaatag acatttttca tccatcgatg gggtctatga tttcgtcctt
     2461 ggatttgtca acgtcgaaaa gggtcaggca accgaattca agctggatcg catgcactgg
     2521 atggccgcac agcttggcaa tccgcatttg ggaagactaa ctgtacatgt agcgggttcg
     2581 aaaggcaagg gctcggtagc aacattgatt gcaaaaacac tcgaatcatc cggactgcga
     2641 accggactgt atacatcacc ccatatcctc tcttggaaag aacgaatcac agaatcaggc
     2701 atagaaatgg ctgatgagct cttgcttaag gctgcggaag aagtctattc ccttgtcgaa
     2761 ggcaggactc aagaagactt tcctggcaat gaattgccaa cattcttcga attgaccacc
     2821 ctgataggct tttgcgcctt tcgtctagct ggtttcgaag cacaggttat cgaggtagga
     2881 ttaggaggac ggctggattc taccaacatc gtatcaccca atgtatgcgt aataacacct
     2941 atagaactcg agcatactca atttcttggt tcgaccatac ctctcattgc cgcagaaaaa
     3001 gcgggaatca taaagagaag tgtacctgtc tgcactattc aaccaaaaac agaggcgttc
     3061 agagtgatca gcaagaaagc cgaagatata ggagctccct gccttctggt cgggagagat
     3121 atctctttgt ccgatataaa gatcaatatc catggcactt gttgtagatt atccgcaacc
     3181 gatactacac catccagcct tcttcgactc atgcgaggtg cttcgattga aatcagcacg
     3241 cctttggtgg gctcgatcta tgctggaaat atggccgttt ctgccctcgc cctttctcag
     3301 ctagacatcg agctgaatac ggaacacatg cttcagggct tttcttcatc atctatgccc
     3361 gcccgatttg agatagtctc acattctcca ttcgttgtgc tggatggagc tcatactcca
     3421 gaatcgattc gaaccaatct ttccacattc ctagagcttt cgaaggctcc acgcatttta
     3481 ttgttcggat gcgctcatga taaaaagcat gatgaaatcg cgaaaataat ctcaccatat
     3541 ttcgacgaaa taatcataac aaggccaggc acatttaaac aaagtgaacc agctattgta
     3601 ttcgatagtt ttcaaaaaca gatgtcgaat agcattatat cagatcgcta tctaatcgaa
     3661 gatacaaata gcgcaatagc caaagctctt agcgatgcaa gggagaaaaa tgctagcctt
     3721 cttgtaacag gctcattcta tctgtgcgca gagttcaaaa agtcgagatt atgctctgaa
     3781 tgaagcctat cattcctccc aaaatacctc caagccatgt aatccaagcc aattcttttt
     3841 ttactacttg aagaacaatg cgttcgacct ctttcatgtc caggctatcg atccgttctg
     3901 agaccatcga agttatatcg aatgcgtcta ttaaagtcga tattctggat tgaatgagtt
     3961 cgattaaacc atcggtaagc gataccgaaa ttctctcgat ttcggaatct tgaattttga
     4021 gaatatccgc gaaggtcttg ccttttgaaa catcctttaa gacctcaata aaaatcgatc
     4081 ccattgtatt ggaagcgctg ctatcggaga catgcatggt tggaacgatg attgaaaaga
     4141 caaatccaga tatggcttcc tcatttgacc ttattcctag aatttcccca aactccagat
     4201 ccgcaagaga gtgatatatg gcttcagctc gcagtcgcaa gtcttcggaa gaatccttta
     4261 gagacagaac aatctgttca gatacaactc tgctcttaga gccgatggag tcatcagcgc
     4321 gctgatatat ctctggaccg gtctttgtgg tttcagcttc gtccttcgaa cgccaattaa
     4381 taattgccct ggaaatagtt ttggaaatac tttgaggagt ggagggatct ttcaagatgc
     4441 gttctatggt ttgtattaga tcctcgatgt tctcgggcat ggcttgagca attttggcat
     4501 cgtatcccgc aatagagata aaaagccttt ggatgggccc tagtctatcc aatgctcttt
     4561 ttacaaatgc ttgagcttcg gtttctagcc ttgccctaaa tccttcacta tgaaaaaaac
     4621 tatcgatata gtccagaaac caggtatata cttgcggaac aagagcatct gtaaaagcca
     4681 taatcgttac atccggagga aggtctataa cggctttcaa aagcggatta aaacgtgatg
     4741 atttagattt cgaggattga aggctctgct catggttaga ttcggaaagc actcttgttt
     4801 gcaaagtaga acaagctttg agtacagatg ctataaattg ttctttagaa actaattctt
     4861 taaaggagag cttgccaatt cgattcagga attcggcgaa gattccctct atggagcttt
     4921 gaacgctctt gcgctccact agatgttgta aagcctcaat tatattgcgt tgaagcgcag
     4981 agacttctga atctattgta gttccattac cagactggac ttctttcgtt attttctggc
     5041 catttgattc cagcagaaga agtgagggct catcatcttt tttttcctcc aggcctggct
     5101 caccaatttg ggaaaaaatc cgtcctgcat cctgattgaa aaaactagct aaagccgatt
     5161 tgatagcctt caagatagtt tgctttatat cagcagactg aattctttga gtgatcacct
     5221 ctgcagtcag gagctcctta gagactgtct ctcctaaact tacagcaagc ttgtccttct
     5281 cgcgcggcag caagcctgga gtaaagggca aggcaatgcc cgcaaatttg attgtcttgt
     5341 atggtctgaa aagcatcttt atagcgagcc agttagtaaa atacccgata attgcaccga
     5401 caagcggtgg aaggatccat gctttgaaaa attccatgct tattccatcc tgagagctgc
     5461 atttcttgct tgcttttcgc tggagtaggt cattacctcc agggctggaa cccatccggt
     5521 ttgcccagct tcttgtattc tgaaccagag tgtccctcga tcgctttccg atgtactata
     5581 cgtgctctct attatccgca aaacggtccc ttttcggaga atggagaggt tttgtgaact
     5641 attggtagga tcttttttca tttgacaata ctgggaagat ataacaagcc attgcgcatt
     5701 tccgcccaga ctattatcct ccggtagccg caagcgaggt ctacatcctg acatggcgca
     5761 caaaacaaat gaagaaatta gaattaatga tgaataaaca aatatcttct tcatactgac
     5821 ataccgcttg atgacgctgc gaaggaaacc atgtagtatt aaacaataac tatgaatatg
     5881 acaatcggca aggcaaaagc aaagccgttt ttgttcttgg tgtttatggt ttgcaaactt
     5941 ggcgctacgc aggcaatcgg ggctcttccg gctctggaat tcggcatggg atttgcttat
     6001 gctaggacga aagtcgaaag cgccgcaaca ggatatcacc atggatggaa tgtctcagct
     6061 gatgcgaatt ggttattccg tatatctctc gaagatgaga atcttataaa agcaaaagca
     6121 gaaaaagaga cttcatcgcg cgtcaatatg atattttcac cgggactgca tgctcaattt
     6181 ataaccatgt cagcttctaa ctggctctcg gatggttcac gctatagagc atgggattct
     6241 attgggcttg ggccagaact gaatcttggc ttagaattca ccaattccat gacaatgcat
     6301 aaaaatggct tcttgttttc tatggcatat cttgcaagct tttctaatta tacacaaaca
     6361 acgctttatt cggcatataa ttcgtggttt ttgaaagtat cttggaatgc cagaattgac
     6421 aatggtgcag cttttttcgc agccctgccc ttggaatatg cattcagagc ggatggcaac
     6481 agcctattat ctgggcttgt aattgggatg agatatgaat tctaaaactc tacatacatc
     6541 cagcaaatat acatatatgt ttatgtgcat aaagatgaaa gatttcgcaa aagagactag
     6601 caaatatggc gggttaatga agggagcatt ttttctgctc gttctatggc cactattagc
     6661 aagctgtgag attttagctc caactaatgc cacattcgaa ggggaaaaat atgcggttgt
     6721 ggtggggata aatgattaca tcgacaatac aaacaatctg attagggatt tagatttctg
     6781 tgccgccgat gccgaggcca tgaaagacat gctgacaggc gcaggctggc aggtggaact
     6841 tatcacggcc gaagcgaacg aatcaataaa gaaaaatgca accaaagaaa aaatcaagac
     6901 ggcatttcaa aaggacaaag atggcttgac agcgtttctc ttctattttt ccggccacgg
     6961 tgatgtacat gaagatcagg cttacattgt gccaagcgat gcctatcgat ctggcaattc
     7021 cgtagagaaa tcaagcatga tctcagctac tgaattgtct atgtggctaa aggatttgaa
     7081 tgccaacaaa atcatgatct tcgactcatg ctactcgggc ggatttgtga atccgggaga
     7141 ttgtatagat gctgctccgg gaaacattga atctgatcaa atttccacaa atatcgaaat
     7201 gtttttccgc tttggcgaga tactcgctca gaactctgct tccgcagcca gaagcccatc
     7261 tacggctcct cttgtgatat ccgcttcagg ctcgaatgaa gcctccatgg aagacgccaa
     7321 tgaaagacca actcatggaa gtggccatgg attctttact tattattttc ttcaatctgc
     7381 cgaagcatca aaagatggct caatgaaagg agatcttgat ggcgatggaa tactctcctg
     7441 tctagaagca tataattatg caaaaaatgc gctcgaaaaa gactggatag cttcccagct
     7501 accgcatatt acaggcggtc ttagggattt cgctctgatc gatttgcgct aggacctata
     7561 acagataagg catgataata attcgaatag ccttcaatcg ataaaatccg tatcttcctt
     7621 tgagaattcg gggaaaaaat agacaagtac accggaactc cgcacgcgca cctgtgcaat
     7681 gctcttcttt gcaagattat cgagttggcg ctgtgcctct tctataggga tgttggcctc
     7741 aatcgccact tcgcctgccg tgaccatgcc attgttagcc ttagcgaggc gaagaataac
     7801 ccgctctgga gtttcggact gtcgaatatg tggaatgaac tcaccccatt ggccatagtt
     7861 ttcatgtcca atagcctctc tcgcagaata ggcaagattc gcttctcgaa cttgtcgagg
     7921 cagagtaata agatcatata ggcctcctaa tcctcctaga ccacctgtaa acatccacaa
     7981 aagcccagtt ccggtcttcc ctaaatagaa acgatggaag cccagggctc caaagccaga
     8041 aataagccag aggaaatagg ctgttgtaag attgtatctc acgctccgct cccaaaaaga
     8101 tcttaattaa actctatggt atttgaccat tatagctatg tcgtgctaaa aaggagtata
     8161 tgaattttat cgcatgacga aaagccatgc accactcttt ctatggagct tttcatgact
     8221 cttcttccaa caataaagca acttctttcc atggaagctg taggtcagcc tgtgatcatc
     8281 aggggatggg tccgcactaa acgcgagaca aaacaagcta tttttatcga agtcaacgat
     8341 ggttcttgcc tgtctaatat ccaatgcgtc ctggattcct ctctcgaaaa aaaccaatcg
     8401 atagctgaga ctatcttgag attatctacg ggagcaagcg tggagataca tggagagctc
     8461 gttccctctc ctgctaaggg tcaaaaggta gaagtcaaat gccaacagat caagctaatt
     8521 ggtgaagcac catcagagcg ctatccgctt cagaaaaaag cacatagcct cgaatttctg
     8581 cgtgaaattg cgcacctcag ggtgcggaca aatacttttg ctgcagttgc ccgcgttcga
     8641 aatcggatgg catttgccat acaccagttt tttcaagagc gtggcttcta ttacatccac
     8701 acaccaatca ttaccacaag tgatgccgag ggagctggcg caatgtttca agttactagc
     8761 ctcgatatcg aagaactctc gaaatcaggt actctcatag actacgataa ggattttttt
     8821 ggtagaaagg cttttctgac tgtatcaggg caactaaacg tcgaaaccta ttgccaagct
     8881 ctttcgcgcg tttacacttt cggccctacc tttcgcgctg aaaactctaa taccacgcgc
     8941 catcttgccg aattctggat gatcgaaccc gagatggcat ttgctcatct agatgacaac
     9001 atcgctctgg caaccgagtt cattcaatat cttgttgcca cagctatccg agattgtgcc
     9061 gaagatgtag ccttctttga ccagcgcata caaccaggct taagagagtc cttagagaga
     9121 attgtacagt caagctttgc tcgaatgagt tataccgagg caattcgaga actcgaagcc
     9181 tcgaatgcaa atttcgaatt taagccctat tggggctgtg atcttcagag tgagcatgaa
     9241 aaattcttaa ctgagaaggt tgctggcgga ccgctggttg taacagacta cccaaaagaa
     9301 atcaaagcct tttatatgaa gcagaatgag gatggcaaga ctgtggcagc aatggatgtg
     9361 cttgtacctc gttttggcga gatcatcggg ggttccgagc gcgaatggag actcgatctt
     9421 ctggaaaagc gaatggaaga gttaggcctt gataaggaac cctaccaatg gtatttagat
     9481 ctgcgacgct atggctctac ccctcattct ggctttggtc ttggattcga gcggctactc
     9541 ctctatataa caggaatgac aaacattcga gatgtaatac ctttcccaag agcaccgcgc
     9601 caagctgatt tctgatcaac aggaaaacat cgatgaagct tcttacagca atcaaaagac
     9661 accaaacatg cataaataag gccagccttt taattatagc tattatctca tgctttttca
     9721 ttttcgattt tccggctgca gccaattcaa tcgagtcgtt cagtagattg ggtgtcgaga
     9781 ttcccaaccc gccttcgctc aatgcgagat cagcgatact tatagatgcc gcaactggga
     9841 ctgtgctatt cgaaaaagag gcagatataa gacttccgcc cgcaagctta accaagcttg
     9901 tcactcttca tatcgttatg gaagagataa aatcaggaag actttcaccg gacgaaattg
     9961 ttgagatcga tccgcaagat tgttcacctt ccatacctta tggttcttcg ctcatgtatc
    10021 tgcagccagg aatgaaggtg agtgtgaaag atctcatgct gggggccgct gtagtatcag
    10081 gaaatgacgc ggcctattcg ctggctcgcc ggattgcagg gtcgaacgaa gaatttgccg
    10141 agatgatgaa caaagaggtc aaggcaatgg gcttccagag cctttgcttt gtggagccca
    10201 gcggactttc ggagttcaat cttgtaaccg cgagagactt tgcttttttc tgcaaaaaat
    10261 acattgaact acatcctaag gcactcaaag aacttcattc tgtacattca attcaattcc
    10321 ctagacctga acacgcaaca tcacaatatc gtcccaatgg tattgtcctt caatataacc
    10381 gcaatcctct tattttcagc tacgaaggag ccgatggcct caaaacaggc tatataatcg
    10441 aagtaggata taatatggct gctacggcga tgagaaatgg taatcgctat attgccgtgc
    10501 tcttgggagg atcttctacc ccttacgccg atggcgctgc cttgcgcaca aaggacacaa
    10561 aggctctctt cgattgggca tttgcaaatt tcgctactgc aagacccggc tacataatgc
    10621 caaagccact tcgtgtctgg tttggcaaat caatttggat agacccagag cctgaaggtg
    10681 aagcagcggc tacggtcccc gttgtttata aagaccgagt aaccgctcga atcgagactg
    10741 ttaaaggcat caaagcccct gtatataaag gacaaaagtt gggtgagatc gtctatgaaa
    10801 tagatggtac gaagataaat tcggtcaatc tggtttcagg tcaggatatt ccgcgtggta
    10861 atttattcag gactctcttg gatagtatac agtatttctt tgcttacata ttcggaaagc
    10921 tctgaactca tcgaaaaaat gacctgttct aagaacaggt cggctctaaa ccggacgcca
    10981 gataaagaaa taagatttca atacccgagt tctttctctg ctttatcgat tgcggcaaca
    11041 aacttgttca tccagatact gccttcctcc ttgtactgcg taagtaagaa tttattcgcc
    11101 gcaggaacta ctatatcttt gaattgtttc attactgctc cggatgggac atagacctgc
    11161 attttcccag aaagcatggg aagacctctt tctgttgagt ctattattct tgagagtccc
    11221 cttcctgcca caattgctgt tcttgcggca tcatccacga ctatcctgag ctctttcgat
    11281 aggctgttgt agaactccat attgataacc catacatagg gagaatatat cagattgcat
    11341 agtgtcgcat atctctgtac ttcgtatagt ctatccatga ctattataga taccggattc
    11401 atctgaccgt ccacgacccc tgctagaaga cttgcatata gttccgacca ggcaatggta
    11461 accggagttg cgcctagcgc tcttataatc tcctggtgaa gtggaatagc catagtcctt
    11521 actttgaggc cttttaagtc tccaggtgat ctaattggcc tctttgaatt cgtgatagcg
    11581 aaaaatcctc ccgattccgc aaaaccaagg acatgaaaac cagctttctc ttctatgtca
    11641 gcagcaagct ctttcccaaa ctgtccatca tatacatgat aagccacatt gtaactcgaa
    11701 aatgcaaaag ggatgttagt gacatcgata agaggataga actgagcaat ggcgcctgag
    11761 gtagagatag aggattgtac ctgcccggat ttgacctgaa taagtgtttc aatttctttc
    11821 cctgtcacgc catcagggta aatgtccact ttgatctgac catcggactt gctctccact
    11881 tcgcttttga ataccgctga catagcagcg gatacaacct caaaaggtgc ctgagtattc
    11941 aggtgtgcta gttttagagt gatttctcca aacgcggaaa atggaaatga tagaattgat
    12001 atgataaaca aggcagctac cacatttttc atggatgcct ccttaagccg atgacatatc
    12061 aggattcgca atttctgcta gagtaacgga ttcgagatat agatcaactg catctttgag
    12121 tcctagccaa aaatgccgag gcagacagcc ttgacctaat aagcagatcg ccgcattgtc
    12181 gacacactcg acaatcctta tctcgccttc cgttgcccgt accacttcgc tcatccgaac
    12241 aagcgccgga tcttttgcaa gcctgtatcc tccatttcta ccccgttcag caatcaagag
    12301 ccctgcattc ttcagttttc caaaaattgc ttctaggtat ttagacggaa tacactctgc
    12361 ttccgcgata ctcgctacac ttgcggattt tacatgcacc agatgtacaa gcgcgcgaat
    12421 agcatattgt gtctttgttg gaacctggaa cattaaaatt cctactaaat aagtctgaat
    12481 atttaatatc gcattaatgg agactttgtc aatcagctca gaagctcagc gtgatcgaag
    12541 ctgcggttga tcgaaagctc ttcaaacaaa aagccataat cagtgaaacc ctcaggcctt
    12601 tcatggcaac catttgcggc aatagatcgt gcaattatac tgcccagacc aggccccatc
    12661 ataaagcctt gtccacacat gccgacaagc tgaaggaaat tcacggctaa atcggggtaa
    12721 cctataatcg gaagaccatc aggagtcatg ggatacatgc cgcgccatgt tcttcgtact
    12781 cgtagattgc gaagcctagg atacagctca agcattcggc gaacaacaag tggaagaaaa
    12841 gtagaagtag aatccttgtc tcgaccccat atctgtggac gaggagtaat acaaaacact
    12901 atctgcccag tagcagcctg atagaaataa taattgccgg actcggcgtc ggagcggaca
    12961 tcgacaatca ttggttccat aaaacgctcg accggttctg tcacacctgc ctcgtggcaa
//
LOCUS       DAOOWY010000052        12912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4845_length_12912_cov_13.008556, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000052 DAOOWY010000000
VERSION     DAOOWY010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 12912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12912
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4845_length_12912_cov_13.008556"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..556)
                     /locus_tag="PK105_05365"
     CDS             complement(128..556)
                     /locus_tag="PK105_05365"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOU61173.1"
                     /translation="MQLAELCPGQRFKVTNVAVGGEIGKRLADMGLVSGAEGEVVRAA
                     FFRGPIQIRLGDYDLIMRRYEARLVDVELLPEAAPQVAGRPPKKALSTMYVDLRPRER
                     RRSHGATTTPSTHGASGGHGIQDAHGKAHGAHDKDPSCLD"
     gene            complement(558..1001)
                     /locus_tag="PK105_05370"
     CDS             complement(558..1001)
                     /locus_tag="PK105_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOU61174.1"
                     /translation="MNELDRFETFLRGRGQKITKPRRIILEAFLNTEGHLSTEDILHE
                     AKKRDPGIGQATVFRTIRLIEDAGLAREAVQEDGARTFEHLADHPHHDHLLCTACGKV
                     IEFLSPAIEKEQQKIFAKYGFKPTGHTMELLGLCPECQAKEKGVQ"
     gene            1181..1699
                     /locus_tag="PK105_05375"
     CDS             1181..1699
                     /locus_tag="PK105_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOU61175.1"
                     /translation="MGKLYYSYQMIHKLAQSIAEQVMNSGFSPDIIVAIGTGGFIPAR
                     ILRTYLGKPILALGIAYYDLNDKPMDYPRKVQWIEEAEKQLAGKRILLVDEVDDSRLT
                     LEYCTKELLAYRPAALAVSVLHNKQKEKRGAFPPGVQYVFVGENLGDDWIVYPWDARD
                     IDEHDKNAALEH"
     gene            1744..3237
                     /locus_tag="PK105_05380"
     CDS             1744..3237
                     /locus_tag="PK105_05380"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU61176.1"
                     /translation="MRHKRSWFCILLSLFLMFLTLDTLVAQQAQAVRNATLENGLEVF
                     VVENHVVPLVTVCIAFRGGAIAHSPETAGLFHLYEHMMFNGNDKYPTKDAFSAALNRM
                     GTTSWNGATGREYINYFISVPSEKLTDAIDFWAHAVINPTLDARVLENEKQVVLNEIR
                     GYHSDPSQIALNGLESRMFRDFPWRKNIDGPESNITRANVTALRDIQKTYYVPSNMAL
                     LIGGDTSLEEVVKLANESFGSWARAPEPLLAEPPHGPIPDGIRLVTSEDQFYRGIAQV
                     EFRWRGPDVTRQTYDTYISDVLLFLLSSPSGPFKSEIMRKVYGLYDAEYIDFVYPTSR
                     DGGNYIFATLMLVQRPAEEEPVLQRVENLKSVVLDEFALIAKDPSAYFGGDEALEEAK
                     TKLVDQNIYALESSGSFVTDTLTFWWSTAGTEYFFDYEENCRKVSWEDISSLIERYLA
                     GESNPNVATLVRLRTSTLNSDPRMDAKISELGYSRLTAQNAFWWQKQ"
     gene            3248..>3562
                     /locus_tag="PK105_05385"
     CDS             3248..>3562
                     /locus_tag="PK105_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61177.1"
                     /translation="MRHFNNDVSRMFVVVVLMVSVAIGACAHTPYVRENAPTYMKTVL
                     SNGIPLYMKVQRANQVFHVSLVLNGASLVTPPEQAGWEKIALATMARSSKNYPYEKAA
                     SIL"
     assembly_gap    3563..3638
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3639..4687
                     /locus_tag="PK105_05390"
     CDS             <3639..4687
                     /locus_tag="PK105_05390"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU61178.1"
                     /translation="LWSDMLVNPSFDQSDFDREKENALLALQAIGQNPWTMTQKIMNE
                     AFFKGHPYGVNPEGTEESIAPMMAGDARDWYKEHFSADRIFLVAIGDFNPAELAYKLE
                     KLLGDIPDLNLGPISKPQEFRIGLSGFGSSSESGLPSGSLITQAHDQSKGIIYLRGDF
                     AAPAPGSDDYFATTLATRIFSDLLFAIVRDKYGAVYTPSAAIRSYTANYGSIMIYKSD
                     APEKIKQYIDEAAALMAQGRVVSVDPTKTEADGYMALSDALDTYKQLYINNYFEAVRT
                     NAAIASMMIRSIKLTGDPADWLKDQVRIEMLDASAIQNAFEKYVINGTFLWVAVGDQA
                     LLDRLDRRAFESIR"
     gene            complement(4732..7758)
                     /locus_tag="PK105_05395"
     CDS             complement(4732..7758)
                     /locus_tag="PK105_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU61179.1"
                     /translation="MPFVLQKESSVPELDSIIRLYRHERTGARLLSVINKDENKVFGI
                     SFRTPPERNNGVVHIIEHSTLCGSRKYPVKEPFVELMKGSLNTFLNAITFEDKTCYPV
                     ASTNLKDFYNLIDVYLDAVFYPNLTEYTFMQEGWHYEVDPVTRELSYKGVVFNEMKGA
                     YSDPDSLHDHLCKCSLFPDTCYGLDSGGDPLEIPTLSYEEFIAYHKKYYHPSNSFIYF
                     YGDDDPEKRLSFMDEWLSPFEAASIDSLPAIQQTFPEPLLIKRNFQANSKEAAKAYTA
                     VNWMLYEHGDMLLSMQALVLFHILTGTPASPLRKALIESGLGEDIAGFGFYEEMRQTA
                     FSIGLKGVEPHNLAKVEELIFSTLERLACDGIDPDTIAASLNTIEFALRERNTGSFPR
                     GLAIMLDALNEWLYDLDPISALSFAEPLEQTKRAAKENHRLFSELIDSLILKNKHRST
                     VRFLPSSGLKNEREEAEKAMLALALASLDDEALRKIEDTAKNLKELQDKPDSPEALAT
                     IPVLSLDDIPGEAPVLPSEPLESATEEFKGSGIYGYYHELSTSGILYLDLGFGFGKLP
                     SRLLPYVSLLGRFLIETGTEKYDFVQLIQRIGMHTGGIRSMVISTTHWAEHRPVPWFF
                     LRAKALPEKVGILSDILLDILTSAQLDNRERVRQIVLEEKAQAEAMLIPASARIVGLR
                     LRSRFNSAYWASERLMGIERLFFLRKLLRNIEEDWPSVLSEIREVHSELIWRDNLLVN
                     ITSDKAILEASAESILRIAQSLPMQAMLAQSLPHRSFPQLDSWVNEASRACYPSFSQG
                     HEDSSPKNEDAHSARIPLETIELQTQVNSVGMIMPLKGLGPIAGGALAATKYLDTSYL
                     WEQIRVIGGAYGGFSSLDLASALLMFLSYRDPNFNQTIEAYRKVATFLESCNLPREEI
                     QKSIIGTIGDMDAYQLPDAKGFNALMNILAGYTQETRQHIRDQILAANMDDFRSFGKL
                     LSEALDRSMIVVMTSPEQAEKALSTLPAPIARLSLQ"
     gene            8053..8589
                     /locus_tag="PK105_05400"
     CDS             8053..8589
                     /locus_tag="PK105_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOU61180.1"
                     /translation="MKASRKAIRFLMVGAMLMAVTASTFAQGFGGMSNQRAKTPGYGY
                     QSVPPVAPKADPYGNRFGDGSRGFANQEKSFLGNKRSMGAGGYGVYHYFQYQKLSAED
                     RAKVDKIIEDSASKVLPLQNELRAAKLNLDNLLWSANPDKAKIDEAIAKIVDIQKQIQ
                     EIRATNILEINKVFQSTN"
     gene            8756..9319
                     /locus_tag="PK105_05405"
     CDS             8756..9319
                     /locus_tag="PK105_05405"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOU61181.1"
                     /translation="MIPMKTNQARLSLFLILIVGIAFLGIFPVAAQAGYGKGMGNQGV
                     MGPTVVREWFRSLNLTDQDLDKLEKILQSRELELVKAQNEIRILQTQIANILVDPDPD
                     LKAIDEAISKSLQYEKIVRMIQIDRQVAIRKVFGEDRWQTILFLVHEARMSEKAGRFA
                     NSFSTKGLSAEEANRYGRLLGMLRRIM"
     gene            9393..10040
                     /locus_tag="PK105_05410"
     CDS             9393..10040
                     /locus_tag="PK105_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU61182.1"
                     /translation="MSSAKKDRVRIEQDKAEAPSLALSSEEETLDFELAARAAKGDKG
                     AFERLIWRWWDRIRGYCASFVGFDEELSEEAVQESLIRIYKALPKWRKESLLGGYIYG
                     ICRTSCADVMRVNARHHARNLSVEEFDSLSMESPHGLGEEDMLRKEASQMLELALKML
                     EPEDRSMLYLHEVDGMGLSELGRMYNLPIGTVKSRLFRARDKMSAMLKEMGYELS"
     gene            10027..10413
                     /locus_tag="PK105_05415"
     CDS             10027..10413
                     /locus_tag="PK105_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61183.1"
                     /translation="MSSAERNKIDLRADLKDTAMRKAPAFPDFAQVYKKAEEQPRKRQ
                     KILISTAIAITALVASFFVGGLWESRSSADVALIDSWSVTPAPIAIGISALEDEDDQI
                     ILTSSKNSTLNLYIQGLWESTSTNGL"
     gene            complement(10467..11924)
                     /gene="proS"
                     /locus_tag="PK105_05420"
     CDS             complement(10467..11924)
                     /gene="proS"
                     /locus_tag="PK105_05420"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOU61184.1"
                     /translation="MPEKITPREADYSQWYIDIVLNAKLADYSPVKGSMVIRPRGYAI
                     WEKIRNEFDRRFKETGHENAYFPLLIPMSFLKKEAEHVEGFAPELAVVTHGGGEELEE
                     PYCIRPTSETIIWAMYRNWIQSWRDLPLLINQWANVMRWEKRTRLFLRTSEFLWQEGH
                     TAHETEKEARQETLRILEIYRDVVESCLAVPVVTGMKSESEKFAGAVETYTIEAMMQD
                     KKALQAGTSHFLGQNFARAFDVQFQNRQGQLDYVWATSWGVSTRLIGAVIMTHSDDKG
                     LVLPPALSPEDAVIIPIYRNDSKAAVIAYAEKLEADLKAAGFRVVLDIDDSYSPGWKF
                     TEWELRGTPVRIEIGPRDIKSNQVVMVRRDTSEKLTTSNTEAASKLRELLKFIQDSLF
                     ERARSFRQENTKPISDLGALIEFFGTETAGTEGAPGGFAESLWCGSPTCEASIKEKTK
                     ATIRCLPLDRQDQIEGPCAICGAPAKYLAIFARNY"
     gene            12015..12842
                     /locus_tag="PK105_05425"
     CDS             12015..12842
                     /locus_tag="PK105_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61185.1"
                     /translation="MTRRNFRFTYWLFSSISLVILVTSIVLTVRIGNDKRLPFFLADL
                     NLYTEYFFLDSPIGMQLAGALECCFLVFAATFMLIAVLFTFQKTVSAEIYLIALWIFL
                     VEAEALRLPMLIIAQRSTHTGSLISLTRLIYGARFSGLISLFISGLYAVGIGKERPQT
                     GCLLALLLGSVFAMLLPISTDRFQSSFMLQAGYSELTIIVTASLIVLNAIDYFVAARQ
                     KEEKSYVFVGITLTVAASSWVWLWNTRDFKWGLIVVIAFGLCIFASIKILHYIYIWK"
BASE COUNT         3376 a         2824 c         3186 g         3450 t
ORIGIN      
        1 gtttcctacc ttgtggtgtg cgcctgttat agcattgaat acactggtct ttccagaatt
       61 tggattgcct gcaagcgcaa tggtgagaat gactctatcc tgcatggatt actcctgaaa
      121 aagtctttta atcgaggcag ctaggatcct tgtcatgggc gccatgagct tttccatgag
      181 catcttggat gccatggccg ccagaagcgc catgcgtgct aggagtggta gtggcgccat
      241 gagaacgcct tcgttcgcga gggcgcaaat cgacatacat tgtggatagt gctttcttag
      301 gcggcctgcc tgccacctgt ggcgcagctt ccggaagaag ctcaacgtcg accagacgag
      361 cttcatatcg gcgcattata agatcgtaat ctccaagcct tatctgtata ggaccgcgga
      421 agaatgctgc acgcaccacc tctccctccg cacctgagac gagacccata tccgcaagcc
      481 gtttcccgat ctctcctcct accgcaacgt tcgtgacttt gaatctctga cccgggcaaa
      541 gttcggcaag ctgcatatca ttggactcct ttttcttttg cctggcactc agggcagagg
      601 ccgagcaatt ccatagtatg acctgtgggc ttaaatccat attttgcaaa aatcttctgt
      661 tgttctttct ctatggcggg gctcaagaat tcgatcacct tgccacaagc agtgcagagc
      721 agatggtcgt gatgagggtg atcggcaaga tgctcgaatg tacgagcgcc atcttcctga
      781 actgcttccc gagctagacc cgcatcttct attaggcgaa tggttctgaa caccgtagcc
      841 tgacctatgc ctggatcgcg tttttttgct tcatgtagaa tgtcttcagt gcttagatgg
      901 ccttcggtgt tgagaaatgc ttcgaggatt attctgcgcg gtttcgtgat tttttgaccg
      961 cgcccacgca gaaaagtttc gaatctatcg agttcattca tttcgattgc cttatccaat
     1021 atcataaatg aaaagaggaa acgaaaagcg ataagcagcg aaaaaaataa tcattctatc
     1081 gctcttcgag aatgataatc attctcagtt aaaagatcaa gatgagttgt cactcaggcg
     1141 atctcttgtc tatgttatgc cattaccgta gaatcgttat atgggaaagc tctattatag
     1201 ctatcagatg attcataagc tggcgcagtc cattgcggag caggtcatga atagcggctt
     1261 ttctcctgat atcattgtag ctatcggtac cggcggtttt attcctgcgc gcattctaag
     1321 gacctatctt ggcaagccga tacttgctct cgggatagcc tactatgact tgaatgataa
     1381 gccaatggat tatcctcgca aggtacaatg gatcgaagaa gccgaaaagc aattggcagg
     1441 caagagaatt ctattagtgg acgaagtgga tgattctcgg ctaactctag aatactgcac
     1501 taaagagcta ctcgcatata gacctgcagc gctcgcggtc tcggttctgc acaacaaaca
     1561 aaaagagaaa cgcggcgcat ttcctcctgg tgttcagtac gtctttgttg gagaaaacct
     1621 tggagacgat tggattgtgt atccttggga tgctcgagac atcgatgagc acgataagaa
     1681 tgctgcgctg gaacattagc ttaaaaactg ggatattagc tcagaaagga gagtaaaaag
     1741 ccaatgagac acaaaaggtc ttggttttgc atattgttgt ctcttttttt gatgtttctg
     1801 acactcgata cccttgttgc ccaacaagca caagccgtca gaaatgccac gctcgagaac
     1861 ggacttgagg tctttgttgt ggaaaatcat gtagtgcccc ttgttacggt gtgtattgcc
     1921 tttcgtggag gcgctattgc tcatagtccg gaaactgccg ggcttttcca cttgtatgaa
     1981 catatgatgt tcaatggcaa cgataaatac ccaaccaagg acgccttctc agcagcgcta
     2041 aatcgtatgg gcacaacaag ttggaatgga gcgacgggca gagaatacat aaactatttt
     2101 atcagcgtgc cttcggaaaa actcacggat gctattgact tttgggcaca cgccgttatt
     2161 aatccaaccc tggatgccag agtcctggag aatgaaaagc aagttgtact taatgaaatc
     2221 cgaggatacc atagcgaccc gtcgcagatc gccttaaatg gcttagaatc gcgcatgttc
     2281 agagattttc cgtggcgaaa gaatatcgat ggtcctgagt cgaatataac aagagcaaac
     2341 gtgacggcgc ttcgagatat tcaaaaaaca tactatgtgc cttctaatat ggcgctcctc
     2401 ataggagggg ataccagtct agaggaagtg gtaaagctag ccaatgaatc ctttggaagt
     2461 tgggcaagag ctccagagcc tttgttagcc gagccgccgc atggacctat cccggatggt
     2521 attcgtcttg taacttcgga ggatcagttc tatcggggca ttgcccaagt ggaattccgg
     2581 tggagaggac cggatgtcac gcgccagacc tacgacacgt acatatcgga tgtgcttctg
     2641 ttcttattga gctcaccatc cggacctttc aaatcggaaa taatgcggaa agtctatggg
     2701 ctctatgatg ctgaatatat agattttgtt tatccgactt cgcgcgatgg gggaaattat
     2761 atttttgcca ctctcatgct agttcagagg cctgcggaag aagaaccggt acttcagcga
     2821 gtggagaatc ttaagtctgt agtcctcgat gaattcgcac tcatagccaa ggatccatca
     2881 gcttattttg gaggcgatga agcgctcgag gaggccaaga caaagctggt agaccagaat
     2941 atctacgcac tcgaatcatc gggatcattc gttaccgata cattgacatt ctggtggtct
     3001 accgcaggca cggaatattt tttcgattac gaggaaaact gccgtaaggt gagctgggag
     3061 gatatttcta gcctaataga gagatattta gcaggagagt cgaatcctaa cgtagctacg
     3121 ctcgtgcgtc tgcgcacaag caccttgaat tcggatccgc ggatggatgc gaagatatcc
     3181 gagctaggct attcgcgctt gactgcccag aatgccttct ggtggcagaa acaatgaggg
     3241 ggcaggcatg aggcatttta acaatgatgt ttccaggatg tttgttgttg tcgtactcat
     3301 ggtatcagtg gcaattggag cttgcgcgca tactccctat gttcgcgaaa acgctcctac
     3361 gtacatgaag acagtgcttt ccaacggtat tccgctatat atgaaagtgc agcgcgcaaa
     3421 tcaggtcttt cacgtttcgc tcgtattgaa tggcgcttcc cttgttacgc ctccggagca
     3481 ggcgggctgg gagaaaattg ctctggcaac catggcgagg agttcgaaga attaccctta
     3541 cgagaaggcc gcttcgatcc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ctctggagcg atatgctcgt
     3661 aaatccttct ttcgatcagt ccgattttga cagagaaaaa gagaatgcct tgcttgcgct
     3721 gcaggcaata ggacagaacc cttggacaat gacacagaaa ataatgaacg aggctttttt
     3781 caaggggcat ccatatggag tcaaccccga aggcaccgaa gaaagtattg cgccaatgat
     3841 ggctggagat gctcgagatt ggtacaagga acatttttct gcggatagga tttttcttgt
     3901 tgctatagga gatttcaatc cagccgagct tgcttacaag ctcgaaaagc ttcttggtga
     3961 cattcctgac ttgaatcttg gaccgatatc aaagccgcag gagtttcgaa ttgggctttc
     4021 gggattcgga tcttcatcgg aatcaggatt gccatcgggg tctctcatta cgcaagccca
     4081 cgatcaatcg aaggggataa tttaccttag gggagatttt gcagcgccag cacccggaag
     4141 cgatgattat tttgcgacaa ccctggcaac acgtattttt tcggatttgc ttttcgcgat
     4201 tgtgcgcgat aaatacggag cagtctacac tccttcggct gccattaggt cctacacagc
     4261 aaattatggc tccataatga tttataaatc agatgcgccg gaaaaaataa agcaatacat
     4321 agacgaagcc gctgctctca tggcgcaggg aagagtggtg tcggtggatc cgaccaagac
     4381 agaggccgac ggctatatgg cgctctcaga tgcgctcgat acctataagc agctctatat
     4441 aaataattat ttcgaagctg ttcgaaccaa tgctgctatc gcctcaatga tgattcgctc
     4501 gataaaactg acaggagacc cagctgattg gcttaaagac caggttcgca tagaaatgct
     4561 agatgcctcg gcgattcaga acgccttcga gaaatatgtg attaacggca ctttcctatg
     4621 ggtagcagtt ggagatcagg ctttgctcga taggcttgac cggagagctt tcgaatctat
     4681 tcgctgatta tgttcagtca gctgggctgg tgggtgctgt ccgtggcgct atcactgcag
     4741 agaaagccgt gcaatcggag ccggcagcgt tgaaagcgcc ttctccgctt gctctggtga
     4801 ggtcattacc actatcatcg aacgatcgag agcctccgaa aggagttttc cgaagctgcg
     4861 gaaatcgtcc atgttcgctg caagaatctg gtcgcggata tgttgacgcg tctcctgagt
     4921 atacccggcc aggatattca taagggcgtt aaatcctttg gcgtctggca gttgataggc
     4981 atccatatct ccaatcgtac caatgatcga tttttggatc tcttctcttg gcaaattaca
     5041 ggattcaagg aaagtcgcta cctttcggta ggcttctatt gtctgattga aatttgggtc
     5101 gcgataagaa aggaacataa gcaaagcgga ggccagatcg aggctggaaa accctccata
     5161 cgctccacca ataactctta tttgctccca gagataggaa gtatccaagt attttgttgc
     5221 cgcaagagct ccaccggcta tagggcctaa cccttttagg ggcataatca tgcctacgct
     5281 attaacctgg gtctgcagct ctatcgtttc gagaggaatg cgagcgctgt gggcgtcttc
     5341 gtttttcgga gaagagtcct catgcccttg agagaaggaa ggataacaag cgcgcgatgc
     5401 ttcgtttacc catgaatcga gttgtggaaa agacctatgg ggtaaagatt gagcgagcat
     5461 cgcttgcata ggcagagact gcgcaatacg gagaatggat tccgcggaag cctcgagaat
     5521 ggctttatcc gaggtgatat tgacgagcag attgtcgcgc catatgagct cgctatgcac
     5581 ctcgcgtatt tctgacaaca ccgaaggcca atcttcttca atgttacgca agagcttgcg
     5641 aaggaagaag aggcgttcta tgcccataag cctttccgaa gcccagtatg cagaattgaa
     5701 tcgagagcga agcctcagac cgacgattcg agcagaagca ggaatcagca ttgcctctgc
     5761 ttgtgctttt tcctcaagga caatttggcg gactcgttcg cggttatcta gttgcgcgct
     5821 ggtcagaata tccaaaagta tgtcgctaag aatgccgact ttttcgggca gggctttagc
     5881 ccggaggaaa aaccaaggca ccggcctgtg ttcagcccaa tgcgttgtag atataaccat
     5941 gcttctaatt ccgccagtat gcatgccaat gcgctgaata agctggacga aatcatattt
     6001 ttctgtgcct gtctcaatta agaaacgacc cagaagactg acatagggca aaagcctaga
     6061 aggcaacttt ccaaaaccga agcctagatc caagtaaaga atgccgcttg tcgaaagctc
     6121 gtgatagtag ccatagattc cagacccttt gaattcttcc gttgctgatt cgagaggctc
     6181 tgaaggcagt acaggggctt ctcctggaat atcgtccaga gagagcacag gaatagttgc
     6241 aagcgcttca gggctatcgg gcttgtcttg aagttccttg aggtttttgg cagtatcttc
     6301 gatttttcta agtgcttcgt catctaagct ggctagggct agtgcaagca tggccttttc
     6361 agcctcttcg cgttcgtttt ttaggccgga tgagggcaga aatctgaccg tgcttcggtg
     6421 tttattcttt aagattagcg aatctatgag ctcactgaat agtctatggt tttctttggc
     6481 agctcttttg gtttgttcaa gcggttcggc aaaagaaagc gctgatattg gatcgaggtc
     6541 gtataaccat tcatttagag catccagcat gatggcaagg ccacggggaa aagacccggt
     6601 gttgcgttca cgcaaggcga attctatggt gttgagcgaa gccgcgatag tgtcgggatc
     6661 gatgccatcg caagcgaggc gctcaagtgt tgagaaaatg agctcttcga cctttgcaag
     6721 attgtgaggt tcaacacctt taagccctat ggagaaagcc gtctggcgca tctcttcgta
     6781 gaagccaaaa cccgcaatat cctctccgag cccggattct atcaatgcct ttcgtagagg
     6841 agaggctggc gtaccagtca agatatggaa gagaacgagg gcttgcatcg aaaggagcat
     6901 atccccatgt tcatatagca tccagttgac cgctgtataa gctttagccg cttcttttga
     6961 attggcctga aaattgcgtt tgatgagtag cggctccgga aaagtctgtt ggatagctgg
     7021 aagtgaatca atggaagcgg cttcaaatgg cgatagccat tcatccataa aggaaaggcg
     7081 cttttccggg tcatcatcgc catagaaata aataaaagaa ttcgaaggat gataatattt
     7141 cttgtgatat gcgatgaatt cttcgtagct aagagtggga atctccagag ggtcgccacc
     7201 ggaatcgagt ccatagcaag tatcgggaaa caggctgcat ttgcataggt gatcgtggag
     7261 gctatctggg tctgaatagg cgcctttcat ttcattgaat actacaccct tatagctgag
     7321 ctctctggta acaggatcta cttcgtagtg ccagccctcc tgcatgaaag tatattcggt
     7381 caaattcggg tagaataccg catctaagta gacatctatg agattataga aatccttgag
     7441 attggtgctt gccaccgggt agcaagtctt atcctcgaac gttattgcgt tgaggaaagt
     7501 atttagtgag cctttcatca gctcgacaaa aggttccttt accggatatt tacgggaacc
     7561 gcaaagagta gaatgctcga taatgtggac tacgccattg tttcgttcag gtggagttct
     7621 gaaacttatc ccgaagacct tgttttcatc cttgttgatg acagatagaa gtcgggctcc
     7681 cgttcgttcg tggcggtata gccgaatgat agaatcgagt tcaggcaccg aggattcttt
     7741 ctgaagcaca aatggcatgc gatactcctc tggaaaatga ttattgaaga gagtatagag
     7801 aagcaaaaag cgcaaggttt cgtctctctt tcctcatagc ttaatgcaat gcgtcataat
     7861 agaaaagtgt ctgcagcata agaaggcttt aacagataac aaagttagat gaggatgacc
     7921 agcaaaaaat aatagttata tcctgtcgag atttattcgc aaaatcacgt caaattatga
     7981 tttttcatgc aaccgatttt tcctttctta cgacattata gtgaacgcaa gaaccttcaa
     8041 gggaggaagg aaatgaaagc atcgagaaag gcaatcaggt ttcttatggt aggtgccatg
     8101 ctcatggcag taactgcgag tacttttgct caaggttttg gtggaatgtc aaatcagcga
     8161 gcaaagactc cgggctatgg atatcaaagc gtgcctccag tagctccaaa agcagatcct
     8221 tatggcaaca gatttggcga tggatcaaga ggattcgcaa atcaagagaa gagttttttg
     8281 ggcaacaagc gatcgatggg tgctggtggc tatggggtat atcactattt tcagtaccag
     8341 aagctatcgg ccgaagacag agccaaggta gataagatca tcgaggatag tgcctccaag
     8401 gttcttcctc tccaaaatga acttcgcgct gcaaagctca atttggacaa tcttctttgg
     8461 agcgcgaatc ccgacaaggc caaaatagat gaagccatag ccaagattgt ggatattcaa
     8521 aagcaaatac aagagattcg tgcaactaac attctagaga taaataaggt atttcaatca
     8581 actaactagt tgaaaaacag ctcagcctag aggctgactg acagactaag cgagtcggtc
     8641 catactaagg aaatgttgct gcaaatggga taccttgtaa aaggccacgt ggctatcgac
     8701 gccgcgtggc tcattataca gaacgatata taagatatat aattggaggt ggcggatgat
     8761 tccaatgaaa acaaatcagg caaggctatc gctttttctc atcttaattg tcggaattgc
     8821 gtttctcggt attttccctg tggctgccca agctggttat ggaaaaggaa tgggaaacca
     8881 gggtgtcatg ggccctacag tggttcgcga atggttccgc agcttgaatc ttacagacca
     8941 agacctcgat aagctcgaaa agattctaca atccagggag ctcgagcttg tcaaagctca
     9001 gaacgagatc agaatacttc agacacagat agcaaatatc ttagtcgacc cagacccaga
     9061 tctaaaagcg atagatgaag cgatctcgaa aagccttcag tatgagaaga tcgtaagaat
     9121 gatacagatc gatcggcaag tcgctatcag aaaagtattt ggcgaagatc gctggcagac
     9181 cattttattt cttgtccatg aagctcggat gtcggaaaaa gcagggagat ttgcgaattc
     9241 ctttagtact aaagggctta gtgctgaaga agcaaatcga tacggccgtc ttcttggcat
     9301 gcttcgcaga atcatgtgat gaaaaatatg aaaaagcatt atattaggaa aagcactata
     9361 ttatgaatag agacaataga atggtttgga ctatgagctc cgcaaaaaaa gatagggtcc
     9421 gaattgagca agacaaggcc gaagctccgt ctcttgctct ttcgtccgaa gaggaaacgt
     9481 tggatttcga gcttgcagca agagcagcaa agggagataa gggagcattt gagcgcttga
     9541 tatggcgttg gtgggaccgc attcgtggct attgcgcgag ctttgtcgga ttcgatgaag
     9601 agctttccga ggaagcagtg caagagtcgc ttattcgtat ctataaagcc ttgcccaaat
     9661 ggcgcaaaga gagtctctta ggcggctata tctatggtat ttgccgaaca agctgcgccg
     9721 atgtcatgcg tgtcaatgcg cgtcatcacg ctcggaatct ttctgtggaa gagttcgatt
     9781 ctctttctat ggagagtccg catggattgg gcgaggagga catgctaaga aaagaggcca
     9841 gtcagatgtt ggaacttgcc ttgaaaatgt tagagcccga ggatcgctct atgctctatc
     9901 tacatgaggt tgatggcatg gggctctcag agcttgggcg catgtacaat cttcctatag
     9961 ggacggtcaa gtcaaggctt ttccgtgcac gagacaagat gagcgcgatg ctcaaggaga
    10021 tgggatatga gctcagctga aaggaataaa atcgatctaa gggctgatct caaagataca
    10081 gccatgagga aagcgcctgc ttttcctgat tttgcgcagg tttataaaaa ggcggaagag
    10141 caacctcgga aaaggcaaaa gatattgatt tcgacagcta ttgcgataac cgcccttgtt
    10201 gccagctttt ttgtaggggg cttatgggaa agccgttctt ctgctgatgt tgcattgatt
    10261 gactcatggt ctgtaacacc agccccgata gctattggca taagcgcatt ggaagacgaa
    10321 gacgatcaga tcatccttac ttcttcgaag aattccacac tgaatctcta tattcagggt
    10381 ctgtgggaga gcacatcgac aaacgggcta tgaaggcctt tgtttgcatt tcatcatcta
    10441 catatcccat atacaaatat taacgctcaa taattgcgcg caaagatagc tagatacttt
    10501 gcaggcgctc cgcagatcgc acaaggtcct tctatttgat cttgtctatc cagcggcaag
    10561 cagcgaattg tagctttagt cttttctttg atagaagcct cacaagtggg agaaccacac
    10621 cagagggatt ccgcgaatcc accgggcgct ccctctgtgc cagcggtctc tgtgccgaag
    10681 aattcgatga gggctccaag atcggaaatg ggctttgtat tttcttggcg gaaagatcgg
    10741 gctcgctcaa acagagagtc ttggatgaac ttaagcaatt ctcttagttt gcttgcggct
    10801 tcggtgttgg aggtagtcag tttttcgctt gtatctcttc gcaccattac aacttgattt
    10861 gattttatat cgcgaggtcc tatttcgatt cgaactggtg taccgcgaag ttcccattca
    10921 gtgaatttcc atccggggct ataggaatcg tcgatatcca acacaactct aaagccagcg
    10981 gctttaagat cagcttcgag cttttctgca taagcaataa cggcagcttt cgaatcgttg
    11041 cgatagatgg gaataatgac ggcatcctca ggagataggg cgggtggcag cacaagcccc
    11101 ttgtcatcgg agtgtgtcat tatgaccgca cctatgagtc tagtggaaac gccccagctc
    11161 gtcgcccaca catagtccag ctggccttgc cgattttgga attgcacatc gaaagctcgg
    11221 gcaaaatttt gtccgagaaa gtgactcgtg cctgcctgca gggctttttt gtcctgcatc
    11281 attgcctcga ttgtgtaggt ctccactgcc cccgcaaatt tttcgctctc gctcttcata
    11341 cccgtgacga ccggtaccgc aaggcagctc tcaacaacat ctcggtatat ttctagaatg
    11401 cgcaaggtct cctggcgggc ttctttttct gtttcatggg cagtatggcc ttcctgccat
    11461 aggaattccg aggttcttag aaagagccgc gtgcgctttt cccagcgcat tacattcgcc
    11521 cactgattta tcagcagagg cagatcgcgc caggactgaa tccagtttcg atacatcgcc
    11581 cagataatcg tttcgcttgt ggggcgtatg cagtatggct cttctagctc ttctcctccg
    11641 ccatgggtca ctacggcgag ctcaggagca aagccttcga catgttctgc ttctttcttt
    11701 agaaagctca ttgggatgag caaggggaaa taagcatttt cgtggccggt ttccttgaat
    11761 cgcctatcga attcgtttcg aattttctcc cagattgcat atcctcgcgg acggatcacc
    11821 atactgcctt tgacaggtga ataatcggca agctttgcat tcaaaacgat atcgatgtac
    11881 cactgcgaat aatcggcttc gcgcggcgtt attttttctg gcatttgcaa cacctcttcc
    11941 agtcgcgagg attcgtcata ctatagtgaa aagaagtgct tcaatcaatt gctggaatga
    12001 agtaggatat aaatgtgaca aggcgcaact ttcgctttac atattggctt ttttcaagta
    12061 ttagcttagt gattcttgta acctcaattg tgttgactgt tcgcattgga aacgataaga
    12121 gacttccttt ttttcttgct gatttgaatc tttatacgga gtattttttc ctagattcac
    12181 cgataggaat gcagttggcc ggagctctgg agtgctgttt tcttgtattt gcagcgactt
    12241 tcatgctgat tgctgtgctt tttacattcc aaaaaacagt ctctgcggag atatatctga
    12301 ttgctctttg gatttttttg gttgaagccg aagctctaag gctcccaatg cttatcatag
    12361 cgcagcgaag cacacacacc ggttcgttga tttcgctcac gagactgata tatggcgcaa
    12421 gattttcagg gcttatttcg ctctttattt ccggtttata tgccgtagga atcggaaaag
    12481 agaggccgca aacaggctgt ttattagctc tccttttagg atcggtgttt gctatgctct
    12541 tgcctatcag caccgatcgc tttcagtctt cgttcatgct tcaggcaggg tatagcgagc
    12601 tcacaattat tgtaactgcc tcgcttatag tcttgaatgc tatagattat tttgttgcag
    12661 caagacaaaa agaagagaaa tcttatgtgt ttgtaggaat tacgctcaca gtggcagcct
    12721 cttcttgggt atggctttgg aacacaagag acttcaaatg gggattgata gtggttattg
    12781 catttggact ctgcattttt gcgtctataa aaatccttca ttatatctat atatggaaat
    12841 agcgtttact gcaacgaacc ttgtgtatac tttaggcatt ctataaggaa ttgtctcaag
    12901 cataggcata gg
//
LOCUS       DAOOWY010000053        12884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4851_length_12884_cov_10.484917, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000053 DAOOWY010000000
VERSION     DAOOWY010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 12884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12884
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4851_length_12884_cov_10.484917"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..242
                     /locus_tag="PK105_05430"
     CDS             93..242
                     /locus_tag="PK105_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61186.1"
                     /translation="MMTGLTGFLFLGKEKKANGAGGPWLRNIEQPGIGALRVGLVLRF
                     WVAWA"
     gene            complement(308..1186)
                     /locus_tag="PK105_05435"
     CDS             complement(308..1186)
                     /locus_tag="PK105_05435"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOU61187.1"
                     /translation="MADTLAALVAKRHPRSLEEYDQSLREVIQQFILLALWRGKFFNE
                     AAFYGGTALRLFHGLDRFSEDLDFTLLAPSLGFRFDPWFEYIKKELAAQGLEVKIENS
                     KKARTIQPAFLRTETKKALLIVGVADKLAAAVPSNKLIKIKFEADTEPPLGFTTENKF
                     LLEPIPFSVKVLTPESLFAGKFHAILEREWKQRVKGRDWYDLVFFVRNNIPVKLDYLS
                     KKLRAFNEQSKSLGYDPNIPLSNDRAISMLEQRIQQLNVESAKNEVYPFVEDKDQLNL
                     WSQEFFLDIAHRIRFV"
     gene            complement(1179..1811)
                     /locus_tag="PK105_05440"
     CDS             complement(1179..1811)
                     /locus_tag="PK105_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61188.1"
                     /translation="MQYSTFKYPELDYLQLIEHLKGYNYPRDRITKLLASGQLIRIKK
                     GLYVDPHSAIPYSKEILANLIYGPSYVSLEYALQQHGLIPEAVRVITSVTTKRKKRYH
                     TPIGDFDYLHLPERYFSPGVEYYAIDSERGYMMAGPEKALFDMLYLHYPDLEYAEIEA
                     HLFENMRIEEADFSRLDFSKIESLLASCHRRSIISLHRFIKSRSRKNENG"
     gene            complement(1984..3171)
                     /locus_tag="PK105_05445"
     CDS             complement(1984..3171)
                     /locus_tag="PK105_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU61189.1"
                     /translation="MRIEHEIITNLEEAMQRRLSILPSALRPFTREYHDLPQTLVLTG
                     PRGVGKTTFLLHHATGKRFLYLSMDNPSLANFPLYDLVRAIFLEGYEGVIIDEVHFAA
                     NWSLHLKALSDDFPRHSIWASDSSSLVLRRGVGDLSRRFVPISMPLLSFREFLALETG
                     QNYPACDPFSPNVPLPLAPTPELLTAFQRYKQRGTRPFYAQSEFEERMMAILDKSLYA
                     DVPFFLPGVTDGNLRLMKAIIGTLAHATIPRVQVRSLCADWSIGSEKLYQILEIMQSV
                     GVLRIIRLENDTKAKTAGQKLFFADPVLYELLAGNAGTAREAFVAFLCESAGWSIEAC
                     RDETKADFVISSPLGIKPVRKYTLEVGGSSKSAKHADFMIRDDADYPARNAIPLWFLG
                     FLY"
     gene            3457..4653
                     /locus_tag="PK105_05450"
     CDS             3457..4653
                     /locus_tag="PK105_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU61190.1"
                     /translation="MERIIEQYRRRIAATNQEFIRSIADTIAWDARLVGIRGARGVGK
                     TTLLLQHIAEAFSNNLNAALYASLDNLWFANHSLIELADNFVKRGGTHLFLDEVHAYP
                     QWSQTIKNIYDDYPDLHMVFTGSSLLEILNAHVDLSRRAMVYTMQGLSFREFLNLQTG
                     ENFPVISLPELLQHHETLAPEIVAHVHPFQYFSGYLQTGYYPYFLEGLDVYPQRLEET
                     VQMILELELPQLRGIEIAYVPKLKQLLHIISESAPFIPNISKLSERIGINRHTLLAYL
                     HYLHEAKLIICLYRDAHGISALQKPDKLYLENTNLMYLLKDARPDIGTIRETFLANQL
                     SYGHELEYSETGDFLVDGQYTIEVGGKNKTRAQIKTAADAYIAADNLEYGNDRKIPLW
                     MFGFLY"
     gene            complement(4937..6313)
                     /locus_tag="PK105_05455"
     CDS             complement(4937..6313)
                     /locus_tag="PK105_05455"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU61191.1"
                     /translation="MERKALDVLIEWKARANRKPLIIRGARQVGKTELVRIFARREFD
                     SLIEINLDEYPQKASLFSDDDIQNVFRFIEIDANQKIVPGKTLLFLDEIQSAPMVLAR
                     LRYFYEKMPALHVICAGSLLDFILSEPDYSVPVGRIEFLYLGPMTFEEFLRARGQEQL
                     HEYLNSYTLATPMPEAIHTKLLGFLREYFVVGGLPGVVKAYIESSLDPIVTSREQQNI
                     LQTYYVDFGKYKKKVNVSFLQDIFKKIPTQIGKTVKYTALNPLAKSLLVKESLDLLEK
                     ARLLYRVFHSDANGVPLGAEVNPSYFKLIFLDTGLVSAALGLKLTDFLPYIELTLVHS
                     GAVAEQFVGQHLLYTNEPWAEPSLYYWNRPSKGSTAEVDYLIQYDGHILPIEVKAGAT
                     GRLKSLQMFVQERHAPLALRFNSDIPSLHHTHTAIAGKEAIPFELLSIPLYLVEQTCR
                     LVAASSKG"
     gene            6515..6820
                     /locus_tag="PK105_05460"
     CDS             6515..6820
                     /locus_tag="PK105_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOU61192.1"
                     /translation="MEQILIQIDDSQIATIAHKFSIQKISIFGSAIRDDFSPNSDIDL
                     LIELSPDRAYSYFDVLEIQEQFERIFQRKVDIVEKNSLRNPFRRESILRSAKEIYAI"
     gene            6810..7163
                     /locus_tag="PK105_05465"
     CDS             6810..7163
                     /locus_tag="PK105_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOU61193.1"
                     /translation="MQYSIEDKAYLWDIVDACEDILQFTKCISFEQFESDKIRRFAVE
                     RQLLVIGEASKRLSSSAKDNFPTVERKKITGLRNIIAHEYGEIITERIWIVVQRNIPE
                     LYKLLKAEALSKVIK"
     gene            7311..8510
                     /locus_tag="PK105_05470"
     CDS             7311..8510
                     /locus_tag="PK105_05470"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU61194.1"
                     /translation="MYELEPGSLLRKGKVVVLYGARRVGKTMLMERVIANLQSPSAQR
                     ALQGSQTAGTGAFNGSIVKVFRGVGDDFELASILGSRKIETYRLFFSPYDIIFIDEAQ
                     YIPQIGACAKLLIDLFPEKSIVLTGSSMLTLSQGSSEPLTGRSVERRLFPISLLELRQ
                     EHEWADIYREIEAYLVFGLYPEVLGLGSAAEKAEYLVNLRNSYLFKDILALEQLRNSQ
                     KLQDIVRLLAFQVGNKVSLNELATQTALSKNTVERYLDLLEKAFIIKRVGAFSRNLRS
                     EIRKSAKYYFYDVGVRNAVINDFRPIQLRNDVGALWENFVVMELLKKYEYEHRYANFY
                     FWRTYDQKELDLVIEENGALSGYEIKLRKSAAKVPKLWLDTYKSEVRVITKEALPTLL
                     EPSTVQV"
     gene            complement(8541..9782)
                     /locus_tag="PK105_05475"
     CDS             complement(8541..9782)
                     /locus_tag="PK105_05475"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU61195.1"
                     /translation="MIPRKDLDRLLKQATNHSLSIIIGARQVGKSTLLGMIRAQLAQQ
                     SEMFNLENPLHLSLFNEGYTSFIRTIRTKLIFIDEFQYCSNISSILKAVYDLNPQIKI
                     YATGSSSMEIQTHLKQSLAGRKLETVLYPLTYGEWLYQEDASQRPPLSPHIEEVTPPD
                     ELEAHRARLREFVRYGALPGLTALSDDLEKREYLFGIYQTYIAKDIKAFLKEESVLSF
                     NKAITWLALNNGAQLKKSTLCSVAGISSRQIERYLDVLAGTFVLALVPPLSTNKGKEL
                     TKTPKFYLYDQGIINSIIQDFRDIDRRPDAGILREQFVFWELKKHLDIRYSLYYWCTA
                     DGKEVDFVLKKDQQLLPIEVKSSWQPPLLPSGLRHFLSFYPETKNAVVLYDGPEYTSR
                     HDHCTIYFAPIHKAFAALSLL"
     gene            9949..10584
                     /locus_tag="PK105_05480"
     CDS             9949..10584
                     /locus_tag="PK105_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61196.1"
                     /translation="MKFEQLLEKLGDHPMFDFAMVCTLFEEKKETVRTGLSRLKRAGK
                     ILELKRGLYTFAEPYRRATLNAAYIANMLYAPSYLSERWALSWYSVIPEKTAWYTSIT
                     TRPTRTFENRFGVFRYRTIKPSLFDSYRIETILSQEVRVATPEKALLDLWYLEGGEWT
                     AARMESFRFEPKAIDTALVIDFARASKIPRLIRAAKYWQTYADSVSGGVLL"
     gene            10581..11372
                     /locus_tag="PK105_05485"
     CDS             10581..11372
                     /locus_tag="PK105_05485"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOU61197.1"
                     /translation="MKHELIALARTVSDPTDRLNVVREYLQALIMRSLHESQAFRSIV
                     FMGGTALRFLYNLPRYSEDLDFSRESAEGYEPVLWMRKIKRDLSYQGYEVSVTWNATH
                     TVHVAWVGFAGIMKEVGLSGHASEKISIKIEIDTNPPQGATMSSSIVNRHMLFAVRYH
                     DLASLMAGKIHAILTRPFVKGRDWYDLLWYESRRPPIEPNPILLRAAMAQSGIEDFKD
                     WRIMIKERSAQVDFGAIASDVAPFLEHHEDARLLTRENLMSLLGV"
     gene            11476..12699
                     /locus_tag="PK105_05490"
     CDS             11476..12699
                     /locus_tag="PK105_05490"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU61198.1"
                     /translation="MKRPENRIIVLTGGRQTGKTTLARTLFPDYQFLSIEDPILRGTY
                     ARLTAAQWRELYPKAILDEVQKEPSLIESIKSVYDQWTEPRYIFTGSSQLLLLEKVRE
                     SLAGRCIIVELYPLTVPELETENWAEPVVPSPFQKLLSAPDNTQEYIKSLLPDFALDP
                     AYAKKLRAWDFYTRLGGYPALVNPELDDEDRYLWLLQYVRTYLERDVRDLAALRDLEP
                     FVRLQRYVAFQTGAILNYSGLAQQIGISTKTVQRYIRYLEMSYQTLILPAWERNKSKQ
                     LVKAPKLHFLDYGVLQAITQKRGTPSGQEFESLVIAELFKQERCIFVDAHFFYLRTFD
                     GKEIDLLIELPEGYFAFEIKMAERALATDTRHLRGLQEMLDKPLLHSFVLSNDPQTKS
                     LGEHITAVHAAYFLT"
BASE COUNT         3515 a         2742 c         3206 g         3421 t
ORIGIN      
        1 tctgtgatca tatattatca aatttgataa taagacgtca agggagaaga aagataaacg
       61 ggataaacag gatttttaga cgggatgaca ggatgatgac tggattgacg ggatttttgt
      121 ttttagggaa agagaaaaaa gcgaacggcg caggtggtcc ttggttaagg aatattgagc
      181 agcctggaat tggcgcgctt agagtggggc ttgtcttgcg attttgggtt gcctgggctt
      241 gagccggacc ggtctggctt tggtctttct cttgcctatc ccgccctatt ggaatatccc
      301 gtaaatccta cacgaacctg atacgatgtg cgatgtcaag aaaaaattct tgtgaccata
      361 agttaagctg atccttgtct tctacaaagg gatacacctc attctttgca gattccacat
      421 taagctgttg tattctttgc tcgagcatcg atatcgctct atcattggaa agaggtatat
      481 tgggatcata gccaagactc ttcgattgtt cattgaaggc tctgagcttt ttggaaagat
      541 aatccaactt tactggtata ttgttgcgca caaagaatac aaggtcgtac caatcccgtc
      601 cttttacgcg ctgcttccat tctcgctcca agatcgcgtg gaattttcct gcgaacaaag
      661 attcaggagt aagtaccttt acgctaaaag ggataggttc aagtagaaac ttgttttcag
      721 ttgtaaagcc tagaggaggt tcagtatcag cctcgaactt tatcttgatg agtttatttg
      781 aaggtactgc tgctgcaagc ttatctgcaa ctcctacgat gaggagtgcc tttttggttt
      841 cagttctaag aaaagcaggc tgaatagtgc gagctttttt gctattttca atctttacct
      901 cgagaccctg ggcagccaat tctttcttga tatattcaaa ccaaggatcg aagcgaaagc
      961 cgaggcttgg tgctagcaag gtaaagtcca ggtcttcaga aaatcgatcg agcccatgaa
     1021 agagacggag cgcagtacca ccatagaacg ctgcttcatt gaaaaatttg ccacgccaca
     1081 gcgcaagcaa gatgaattgc tggatgactt cgcgtaggct ctgatcgtat tcttcaaggc
     1141 tccttggatg tcgctttgcg acgagcgcgg caagtgtatc agccattttc atttttcctt
     1201 gagcgagatt tgatgaatct atgtagggag ataatcgaac gacgatgaca ggatgcgagg
     1261 agtgactcaa tttttgaaaa gtcgagtcga gagaaatctg cctcctcaat gcgcatattt
     1321 tcgaacagat gggcttcgat ctcggcatat tcaaggtcgg gataatgcaa atagagcata
     1381 tcgaatagcg ctttttctgg tccggccatc atatagcctc gttcagagtc gatcgcataa
     1441 tattctactc ctggtgaaaa atatcgttct ggcagatgca gatagtcaaa atcaccgatt
     1501 ggtgtatggt agcgcttttt tctcttggtg gtgactgagg ttatgacacg cactgcttca
     1561 gggatgagtc catgttgctg aagggcgtat tccaaggaaa cataggaggg gccatagatg
     1621 aggtttgcga ggatttcctt ggaataggga atagcggaat gagggtctac atagagccct
     1681 ttcttaatgc gaataagctg gcccgatgcg aggagcttcg tgattctatc gcgaggataa
     1741 ttgtagccct ttagatgttc tatgagctgc aggtaatcga gctctggata tttaaacgtt
     1801 gaatactgca tcaagcatcc tcagattttg actccaatgt cgacagtata tcgactttcg
     1861 agtcaaatag caagaagagc ttagaccaga gtatgtgcct gcctatgccg atagcgtttc
     1921 agggggagtg ctgctatgaa gcacgaacgc attgcattgg ctcacaaggt tcgtagcagt
     1981 atttcaataa agaaagccta aaaaccaaag gggaatggca tttcgggcag gataatcggc
     2041 atcatcccga atcataaaat cggcatgttt tgcagatttc gaggaaccac caacttcgag
     2101 cgtatatttt cttaccggtt ttatgcccag aggagaagag atgacaaaat ctgcctttgt
     2161 ttcatcacga cacgcttcga tgctccaccc cgcactttcg cacaaaaagg caacaaaggc
     2221 ctctcgcgct gttcctgcat tgcctgccaa aagctcgtag agcacaggat cagcaaaaaa
     2281 gagcttttga cccgcagttt tcgccttggt atcgttttcc agcctgatga tgcgcagaac
     2341 acctactgat tgcataattt cgagaatttg atagagtttt tcgctcccta tcgaccaatc
     2401 tgcacacagc gaacgtactt gcacacgggg gatagtcgca tgcgcaagcg ttccgatgat
     2461 cgctttcatg agcctcagat tgccgtcggt gacaccaggc aggaaaaatg gcacatcggc
     2521 atagagcgat ttatccaaga tcgccatcat tcgctcttca aattccgact gtgcataaaa
     2581 tggacgagta cctctttgct tatagcgctg aaatgccgta agaagctctg gagtaggagc
     2641 caggggcaag ggaacatttg gcgagaaagg gtcgcaggct ggatagttct ggcctgtttc
     2701 gagggcaagg aattctcgaa aggagagaag cggcatcgaa atcgggacaa agcgacgaga
     2761 gagatcgccc accccgcgcc gcagcacgag cgaagatgaa tcgctcgccc atatcgaatg
     2821 gcgtggaaag tcatcgctga gcgccttgag gtgaaggctc cagtttgcag caaaatgcac
     2881 ttcatcgata atgacgcctt cgtatccttc caaaaaaatg gctcggacga gatcatagag
     2941 gggaaagtta gcgagcgaag gattatccat tgaaagataa agaaagcgct tgcctgtcgc
     3001 atgatggaga agaaaggtcg tcttgcccac gccgcgcgga ccagtgagca ccaatgtttg
     3061 aggaaggtca tgatattctc gcgtaaatgg acgaagcgcg gaaggcaaga tggaaagccg
     3121 ccgctgcatg gcttcctcga ggttggtaat tatttcatgt tcgatacgca tagtagtgat
     3181 tatatgtgaa ctttgtatct gtgacaagta caaaattcat aaatcgtatc tgtgatagat
     3241 acaaatagca atctgggcgc tgcggacttt ttggcttttt ggctctccta ctctataaaa
     3301 tatcctgtaa atcaagtaaa attctgttgg cctgtcgaag catcctgtgt atccttcaaa
     3361 ggttctgtgg cattttgttt atccaaataa ccaaaaatag agcaaaaatg gttattgcac
     3421 tattcaatct tcttgagttt gtgatatagt ttgatcatgg aacgaattat agaacagtat
     3481 aggcgaagaa tcgctgccac aaaccaggaa tttattcgct cgatcgccga cacgattgcc
     3541 tgggatgctc gccttgtcgg tatacgcggc gctcgcggag tggggaagac gacactattg
     3601 ctgcaacata tcgcggaggc ttttagcaac aacctcaatg ctgcgctcta tgcgagcttg
     3661 gataatctat ggttcgcgaa tcatagcctc attgaactcg ctgataattt tgtaaagcga
     3721 ggtggaactc acctttttct cgatgaagtg catgcatatc cgcagtggtc gcagacaatc
     3781 aagaatatct atgatgacta tcctgatctg catatggtat tcactggctc ttccttgctg
     3841 gaaattctca atgcgcacgt agatttgagt cgacgcgcga tggtgtatac gatgcaggga
     3901 ttatcgttta gagagttcct gaatctccag acaggggaaa atttccccgt gatatctttg
     3961 ccagaactcc tgcagcacca tgaaactttg gctcctgaga ttgttgctca cgtgcatcct
     4021 tttcaatact tctctgggta tctgcagact ggatattacc cttattttct tgaggggctc
     4081 gatgtgtatc cgcagcgcct tgaagagaca gtacagatga ttttggaatt ggaattaccc
     4141 caattgaggg gcatcgagat tgcgtatgtc ccaaaactca agcagctttt gcacatcatt
     4201 tcagaatctg cgccatttat tcccaatatc tcaaaattga gcgaacgaat cggtatcaat
     4261 cgacacacgc ttcttgccta tttgcactat cttcatgaag caaagcttat catctgcctg
     4321 tatcgcgacg cccatgggat cagtgcgctg caaaagcccg acaaactcta tcttgaaaat
     4381 accaacttga tgtatctctt gaaagatgca aggccagata taggaacaat tcgagagact
     4441 ttccttgcga atcagctttc ctatggacat gagctcgaat attcagaaac gggtgatttt
     4501 cttgtcgatg ggcagtatac catcgaggtc ggcggaaaaa acaagacacg agcgcaaatc
     4561 aaaaccgctg ctgatgcata tatcgcggcg gacaatcttg agtacgggaa cgatcgaaag
     4621 attccgctct ggatgtttgg gtttttgtat tgaattttgg tcgggatgaa caggatattc
     4681 ttcaacagga tggcatgatg aagactggat tgacgggatt tttctttggc aaaagaaaga
     4741 gaaaaagggg gcagcgcgcg caggctctgg ttgatgagta ttgagcagcc tggacttggc
     4801 gcgcttagat aggaagggaa gatacagacg atcatttaca aaaatataca tttatgtgta
     4861 ttatcgtaat cgatgaaatt tgagcaatta taagaaagcg tatacacaag gttcgtagca
     4921 gtattataag aatccgttat cctttcgagg aagctgcgac aaggcggcaa gtctgttcta
     4981 cgagatacag aggtattgag agaagctcga aggggattgc ttcctttccg gcgatcgcgg
     5041 tatgagtatg atggagtgaa ggtatatcac tattaaaacg gagcgcgagg ggagcgtgcc
     5101 tttcctggac aaacatctgc aaagatttga gccttcccgt tgcacctgcc tttacctcaa
     5161 tcggaaggat atgcccatca tactgaatga ggtaatctac ttctgcagtc gatcccttac
     5221 tcggtctatt ccagtaatag agcgaaggct cagcccaagg ctcattggtg tacaaaagat
     5281 gctggcccac aaattgctct gcaactgctc cagaatggac aagggtgagc tcgatgtatg
     5341 gcaaaaaatc ggtgagtttg aggcccagcg cagcgcttac gaggcctgta tcgagaaaaa
     5401 tgagcttgaa ataggaagga ttgacttctg cgcctaaggg gacgccattt gcatcggaat
     5461 ggaatacacg gtatagaagg cgtgcctttt ctagaagatc aaggctttct ttgacaagaa
     5521 gcgattttgc aagcggattc aatgcagtat atttgaccgt ttttcctatt tgtgtaggaa
     5581 ttttcttgaa tatatcttgc agaaaagaca cattcacttt ctttttgtac tttccaaagt
     5641 cgacatagta ggtttggagg atgttctgct gttcccgaga agtgacaatg ggatcgaggc
     5701 tcgattcgat ataggctttc accacaccag gaagaccacc taccacgaag tattctctta
     5761 aaaagcccag caattttgta tgaatcgctt cgggcattgg cgtggcaagc gtatacgaat
     5821 tgagatactc atgaagttgc tcctgccctc gcgcacgcaa gaattcctcg aaggtcatcg
     5881 gccctaagta cagaaactca atgcgtccaa cgggcaccga atagtcaggt tcagagagga
     5941 taaaatctag gagcgagcct gcacagatga catgcagcgc gggcatcttt tcgtagaaat
     6001 agcgcaggcg agcaagcacc attggcgctg actgaatctc atcgagaaag aggagcgttt
     6061 tgccaggcac aattttttgg ttcgcatcga tttcgatgaa gcgaaagacg ttctgtatat
     6121 catcgtctga aaagaggctt gctttttgag gatactcatc gagattgatc tcgatgagcg
     6181 agtcgaactc ccggcgagca aatatacgaa cgagctcagt cttgccgact tgtcgagcgc
     6241 cgcggattat gagaggcttt ctattggctc tcgccttcca ttcgatgagc acatcgagtg
     6301 ccttacgttc cataggagat attataggac atttttatat ataatttcaa gcgatttagg
     6361 atattttgat aggataaata aatagataga tagataaata gatagatagc gatgcgcaat
     6421 gattgctctc catacctaag acttgaaaaa tcgtcaaata ataaggaatt ttgaggtaat
     6481 tgacaaaata taagaggaga aagatatttg atttatggaa caaatcttga tccaaatcga
     6541 tgattcacaa atagccacaa tcgctcacaa gttttctatt cagaaaatat ccatatttgg
     6601 atctgctatt cgcgatgatt ttagtcctaa tagtgatatt gatttgctta tagaattatc
     6661 ccctgatagg gcatattctt attttgatgt tttagagatt caggaacaat ttgagagaat
     6721 atttcaaaga aaggtggaca tagtagaaaa gaatagtctg cgaaatcctt tcagaagaga
     6781 atcgattctt agatctgcaa aggagattta tgcaatatag cattgaagat aaggcttact
     6841 tatgggacat agtcgatgca tgcgaagata ttctacaatt cacgaaatgc attagttttg
     6901 aacaatttga aagtgataaa attcgtaggt ttgcagtaga acggcagctt ttggtcattg
     6961 gagaggcatc aaaaaggcta tcttcatcgg ccaaggataa ttttccaaca gtcgagagga
     7021 agaagatcac ggggttaaga aacattattg ctcatgaata tggagaaatc ataacggaaa
     7081 gaatctggat cgtcgttcaa agaaatattc cagaactata taaattatta aaggccgagg
     7141 ctctttcaaa agtcattaag taaagcagtt ttctaatgca aagcggggac gagccataga
     7201 taatataatt aatattgtca tttaactgac aatattttgc ctaatttggt caatttattg
     7261 acaatttttc tattaagcgc gatacttgcc gtatgcaatg gattccgcgg atgtacgaat
     7321 tggagcctgg ctcgcttctt cgaaaaggca aggtggtagt gctctatgga gcacggcggg
     7381 taggcaagac aatgttgatg gagagggtaa tcgcgaacct gcaaagtccg tcggcgcaac
     7441 gtgcgcttca gggttcgcag accgcgggta ccggcgcctt caatggttca atagtaaaag
     7501 tatttcgagg tgtaggcgac gacttcgagc tcgcttcgat tctcggttcg agaaaaatcg
     7561 aaacctaccg gcttttcttt tcgccatacg atatcatctt catcgacgaa gcacagtata
     7621 tccctcaaat cggcgcgtgt gcaaagctgc tcatcgatct ctttccggaa aaatcgattg
     7681 tgctcactgg gtcgagcatg ctcacactgt ctcaagggtc ttctgaaccg ctcacaggaa
     7741 gaagtgtaga gcgacgcttg ttcccaattt ctcttttgga attgagacag gaacacgaat
     7801 gggccgatat ttatcgcgag atcgaggcgt acctcgtctt tggactctat cctgaggtgc
     7861 ttggcttggg tagcgccgca gaaaaggccg aatacctggt gaatctacgc aatagctatt
     7921 tgtttaagga cattttggcg ctggaacagc ttcggaattc acagaaattg caggacattg
     7981 tgcggctgct tgccttccaa gttggaaaca aagtttctct caacgagctt gccacacaga
     8041 cagccttgag caaaaacacg gtagagcgct atctcgatct gcttgaaaag gcgtttatca
     8101 tcaaaagagt tggggctttt tcgcgcaatc ttaggagcga aatacgcaag agcgctaaat
     8161 attatttcta tgatgtgggt gtgaggaatg cggtgatcaa cgactttagg cctatccagc
     8221 tgcgcaacga tgttggtgct ttgtgggaga atttcgtagt gatggagctc ttaaaaaaat
     8281 atgagtatga gcaccgctat gccaattttt atttttggcg aacctacgac cagaaagagc
     8341 tcgatcttgt gatcgaggaa aacggcgcac tttctggcta cgagatcaaa ttgcgcaaaa
     8401 gcgcggccaa ggttccgaag ctatggctcg acacctacaa gagcgaggtg cgcgtgatca
     8461 ccaaagaagc tctgcctacg ctcttggagc cgagtaccgt gcaggtgtag aaaatctaag
     8521 acacaacaat cccgttgcca ttataaaagg ctgagggctg cgaaggcttt gtggataggc
     8581 gcaaaataga tcgtacaatg gtcatggcgg cttgtatatt ctgggccatc gtaaagcacc
     8641 accgcatttt tggtctctgg gtagaagctc agaaaatggc gtaagccaga aggaaggaga
     8701 ggaggctgcc aggaggattt gacctcaata ggcaggagct gctgatcctt tttgagcaca
     8761 aagtcgactt ccttgccatc ggcagtgcac cagtaataga gcgaatagcg aatgtcgaga
     8821 tgttttttga gttcccagaa gacaaattgc tctctgagga tccctgcatc gggccttcga
     8881 tcgatatccc taaaatcctg gatgatgcta ttgataattc cttgatcgta gagatagaac
     8941 ttcggtgttt tggtgagctc ttttccttta ttggtggaaa gcggtggcac gagcgcgagc
     9001 acaaaagtac ccgcaaggac gtcgagatag cgctcgatct gtcggctcga gatgcctgcg
     9061 acgctgcaaa gcgtactttt cttgagttgt gcaccattgt tgagcgcgag ccaggtgatc
     9121 gctttattaa acgagagtac cgattcttcc ttaagaaaag ccttgatgtc ttttgcgatg
     9181 taggtttgat agattccgaa gagatactct ctcttttcaa ggtcatctga aagcgcagtg
     9241 agccctggaa gtgcaccata gcgcacaaat tcacggaggc gtgcgcgatg cgcttcgagc
     9301 tcatcgggcg gtgtgacttc ctcgatatgg ggcgataaag gcggcctctg cgatgcatcc
     9361 tcttgataga gccattcgcc ataggtcaaa ggatagagga ctgtctcgag ttttcgacct
     9421 gcaagcgact gcttgagatg cgtttgaatc tccatagagg atgaacctgt cgcataaatc
     9481 tttatttgag gattgagatc atataccgct ttcagaatag aagaaatgtt gctgcagtac
     9541 tggaattcat cgatgaaaat aagttttgtg cgaatcgtgc ggataaatga ggtatatcct
     9601 tcattgaata aggagagatg aaggggattc tcaagattga acatttcaga ttgctgcgca
     9661 agttgcgctc gaatcatgcc cagcagtgtg gatttgccga cttgtcgcgc accgatgatg
     9721 atgctgagag aatgattcgt agcctgcttt agaagcctat cgaggtcttt acgagggatc
     9781 atgcggatat tttatacgaa agcgtgaaaa atatccagat attttttgcg atttcgtgag
     9841 aaatatctcg acaggatgga gcagtctgga cttggcgcgc ttagatagga agggaagata
     9901 cagacgatca tttacaaaaa tatacattta tgtgtattat cgtaatcgat gaaatttgag
     9961 caattgttgg agaagcttgg cgaccatccg atgttcgatt ttgcgatggt ctgcacgctc
    10021 tttgaggaaa agaaggagac cgtgcgaact ggactctcga ggctgaagag agctggcaag
    10081 atcctagaac tcaagcgagg gctctacaca tttgcagagc cgtatcgccg cgctaccctc
    10141 aatgcagcgt atatcgcgaa tatgctctat gcgccttcct atctctctga gcgatgggcg
    10201 ctctcttggt atagtgtgat ccctgagaaa actgcatggt acacgtccat cacgacgcga
    10261 cccacacgga cttttgagaa tcgctttggc gtgtttcgct atcgaaccat aaaaccatcg
    10321 ctttttgatt cctaccgcat cgagactatt ctctcgcaag aggtcagagt ggcaacacca
    10381 gaaaaggcat tgctcgatct atggtacttg gaaggtggcg agtggacggc agcacgcatg
    10441 gaatcgtttc gattcgagcc aaaggcgatc gataccgccc ttgttatcga tttcgcacga
    10501 gcctcgaaga ttccaaggct cattcgggca gcaaaatact ggcaaaccta tgccgatagc
    10561 gtttcagggg gagtgctgct atgaagcacg aactcattgc attggctcgc acggtttctg
    10621 acccaaccga tcggctcaat gtggtacgag aatatctcca agcgctgatc atgcgaagtc
    10681 ttcacgaatc gcaggccttt cgctcaatcg tgtttatggg aggaacggcg ctgcgatttt
    10741 tgtataatct gccgcgctat tcagaggacc tcgatttttc gcgtgaatcc gccgagggat
    10801 acgagcctgt gctttggatg cgtaaaatca aaagggattt gagctatcag ggctatgagg
    10861 tctcagtaac atggaatgct acacataccg tccatgtcgc gtgggttggt tttgcaggaa
    10921 ttatgaaaga agtggggctg tcgggccacg catctgaaaa gatttcaatc aagatcgaga
    10981 tcgataccaa tccaccacag ggcgcaacga tgagctcgag catcgtgaat cgccacatgc
    11041 tttttgcagt gcggtatcat gaccttgcat cgctcatggc gggcaagatc catgcgatcc
    11101 tgacgcggcc gtttgtaaag ggccgcgatt ggtatgatct gctctggtat gaaagtaggc
    11161 ggcctcctat agagccgaat ccgattctgc tgcgtgcagc catggcgcaa agtgggattg
    11221 aggattttaa ggattggcga atcatgatca aggaacgcag cgcacaggta gattttggag
    11281 cgattgcctc ggatgttgcg ccttttcttg aacaccatga ggatgcacgt ttgctcacca
    11341 gagagaactt gatgagcttg ctgggtgttt gaaatgtatt ccataaattg gcctaattta
    11401 tggacttttg ctccataata tattataatt ttaggcatga agaaccgtac aatcgtttct
    11461 tttttgatga atcgcatgaa aaggccagaa aatcgcatca tcgtcctgac aggggggcgc
    11521 cagaccggta agacaacgct cgcgcgcacg ctttttccag attatcagtt tctgtccatc
    11581 gaggatccga ttttgcgagg cacttacgca cgcctcactg cagcacaatg gagagagctc
    11641 tatccaaagg ccattctcga tgaggtccag aaagagccat cgctcattga gagtatcaaa
    11701 tcggtctacg accagtggac tgagcctcga tatatcttta cgggctctag ccagcttctt
    11761 ctattggaaa aagtccgcga aagccttgcc ggtcgctgta tcattgtgga actctatccg
    11821 cttacagtgc cagaactcga aactgaaaat tgggctgagc ccgtggtgcc ttcgccattt
    11881 caaaaactgc tgagcgcacc agataatact caagaatata tcaaatcgct gttacccgat
    11941 tttgcgctcg accccgcata tgcgaaaaaa ctcagggcgt gggattttta cacgcgcctt
    12001 ggcggctatc cggcgcttgt aaatcccgaa ctcgatgacg aggatcgcta tctgtggctt
    12061 ttacagtatg tgcgaaccta tcttgaacgc gatgtgcgcg atctcgcagc gttaagggat
    12121 ttggagcctt ttgtaaggct tcagcgctat gtcgcgtttc agacaggtgc tattttgaat
    12181 tattcaggcc tcgcacaaca aatcggcata tcgaccaaga ctgtccagcg ctatattcgc
    12241 tatctcgaaa tgagctatca gacgctgatt ttgcctgcct gggagcgaaa caaaagcaag
    12301 cagcttgtta aggcaccgaa attgcatttt ctcgactatg gggtgctcca agcgatcaca
    12361 caaaagcgcg gcacccctag tgggcaagaa tttgaaagcc tcgttatcgc ggagcttttt
    12421 aaacaggaac gctgcatctt tgtggatgcg cacttctttt acttgcggac ctttgatggt
    12481 aaagagatag acctcctcat tgagctaccg gaaggttatt ttgccttcga aatcaagatg
    12541 gcggagcgcg cgctcgcgac cgatacccgt catcttcgtg gtctgcaaga aatgcttgac
    12601 aagccgcttc ttcattcgtt tgtgctttcc aatgacccgc aaacaaaaag tctaggggag
    12661 catatcaccg cggtgcatgc ggcgtatttc ttaacatgat tactgctacg aaccttgtat
    12721 aaaaagtaga agcaggatgg agttatgagg gcgataaagg caatataaag aaaactctgg
    12781 ctcccttcct tccccactcc ccttaaaaat aaaccagagt atgtgccttc ctatgcctat
    12841 gcttgagaca attccttata gaatgcctaa agtatacaca aggt
//
LOCUS       DAOOWY010000054        12617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_4979_length_12617_cov_28.920713, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000054 DAOOWY010000000
VERSION     DAOOWY010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12617
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4979_length_12617_cov_28.920713"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK105_05495"
     CDS             <1..194
                     /locus_tag="PK105_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M24"
                     /protein_id="HOU61199.1"
                     /translation="QTREIIRENQAFTWNPSITGAKSEDTMLATKNGPIVLSEPSIFP
                     VLTIERNGYVFKRPDILEL"
     gene            320..1291
                     /locus_tag="PK105_05500"
     CDS             320..1291
                     /locus_tag="PK105_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOU61200.1"
                     /translation="MKAKKAVLLISVLLLLSTLCLGAQPKEKFPTREIEIMVPWAAGG
                     ATDLMVRIFSPVIQKYLGVPILITNKPGGAAVPGYVEAMTKKPDGYYLVAWATPSITV
                     THMQKTPYDANSFEPIINIVTAPVWFLAPASSPYNNLKDLVEDAKKRPGRINLGNAGA
                     GGGTHMIALAFESAVGVKFNHVPYSGGAPAVTAAVAGQVDVITCGPPEGVPQLAAGQL
                     KCLGVFSEKRLEEFPNYPTAKELGYNFAMGQWRGLAAPKGTDPERIKIIHDAFKAAMS
                     DPEVIKLAQQSGLLFDYRNTEDFKKLIQEEDAYYMKIVKENKLGSKY"
     gene            1482..1985
                     /locus_tag="PK105_05505"
     CDS             1482..1985
                     /locus_tag="PK105_05505"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOU61201.1"
                     /translation="MAKADIVLGIIFAALSIVFYIGTLSFPKFSIGINPKVYPLFIII
                     STFALSVMLMVQGIIKLRKERPPSTKTSAEKGISKPSSCDVSLPKGKTALKLIALAIV
                     ILAYVIGFEPLGYIIVTPLLMAFTMYLFGERKPLRIIVVSILVPVILYWVFRTVFRVP
                     LPRSIIW"
     gene            1999..3480
                     /locus_tag="PK105_05510"
     CDS             1999..3480
                     /locus_tag="PK105_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOU61202.1"
                     /translation="MTAIINALLYVLANLHFLIIGVAGGIIVGAIPGLSGSTGIILML
                     PFLIYIDPPAALIMMSGLFCGSMFGGSIASILVSTPGTPSAAATVLDGYPLAKKGEAG
                     KAMGVALIASTLGGIFSTLCLIFLSPQLAKIALKFGPPEYFALMIFGLTIMASVSSAS
                     LTKGFLSGFFGLLLSMVGLNPTLGTERFTFGVTRLLTGFSMMPVLIGLFAVSEVFAQL
                     ELMSEGKIVRLSKKHKIGSIMPTKKEMRGLWKVILGSSVIGTIIGIIPATGGSIASFM
                     AYNEAKRFSKEPDSFGTGNLAGIAAPEAANNATTGGAMIPLLTLGIPGDVVTSVMLGA
                     LMIIGVQPGPLLFSQSPQIITDLFIGFLLAQFIMVALGYLSIRASNLILRVPLGILYP
                     TILCLCLLGAYSLGNSVYDVMLALVFGIVGYFMKKYGFSAPSVILGLILGPIAEQELS
                     RALIISHGDWTVLIRSPLAIMFYAFAVASIFYSFRSFKRSKTK"
     gene            complement(3586..4311)
                     /locus_tag="PK105_05515"
     CDS             complement(3586..4311)
                     /locus_tag="PK105_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOU61203.1"
                     /translation="MYVNLSSKKQRSQEVASHIKELIRDEKLKPGEKLPNELELCSLF
                     SVSRPTIREAVKILISQGIIEIRRGKGTYVTEVPGIAQDPLGLDFITTPNLRLDIIEA
                     RLLIEPGAARLAAQKADSNDIAKIAASVKAMEEVVYRHKVEINAELDFHRSIAEATRN
                     PVIMRIIPIIMESIEKTYQDAPRTSEDHRHAFEEHKLIYKAIADHDADGSFAAMQSHL
                     EASYKRTLARQVPSAKNKKGASD"
     gene            4427..5458
                     /locus_tag="PK105_05520"
     CDS             4427..5458
                     /locus_tag="PK105_05520"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOU61204.1"
                     /translation="MKQNEITRPIELLDDEGHITEEGWARRPFWRYDRSRIKAPWYRI
                     KEWDYYCVLSQKRGYGIAFTVSDLGYAGLSSIVWLDFVKRTFTPVDSLKLLTLGKLGH
                     SPVPERGDLHYKDKKLNISYLIGDGERRIVAESPSFGEGKGLKCDIRLAQDMSADSMN
                     IATSWKENRRAFYYNHKVNCMPALGEVIIGNETFKFEPEDSFGVLDWGRGYWTYRNRW
                     FWGSASGLYEGVPIGWNLGYGFSDRTPASENMIFYNGRAHKLEEIEFHIDPENYLAPW
                     KITSSDGRFEMNFEPVLNRHSKFKVLMLSSIQNQLFGYYSGQLILDDGRTLHIDKILG
                     LAEDVLNQW"
     gene            5545..9339
                     /locus_tag="PK105_05525"
     CDS             5545..9339
                     /locus_tag="PK105_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU61205.1"
                     /translation="MRIIADLHIHSRFSRATSPKLTLPYIERWARIKGINLVGTGDCT
                     HPAWLKELNEQLEPTEGGFFKLKDSVREEFDASKALMEGLPDSKLWAAWNERLQPGRP
                     SQGSVRFVLTGEISTIYTRDGKTRKIHHIVILPDLKAAAAFQARLSRIGNISSDGRPI
                     LGMDSRDLFTALLDSDERSILIPAHIWTPWFSALGARSGFDSIEECYGGELSSRIGAI
                     ETGLSSNPPMNWAVSSLDEFAIISNSDAHSPEKLGREATIIDMEESFASLSEALTRKE
                     SDSTETHSNAAGQAAASATRAAIDAVAAATTGTVLGTIEFFPQEGKYHYDGHRACRIF
                     LSPQESARLSGRCPVCGKPITPGVMRRVTELADRPVDENAPCPADFEGTNRRPYWQLI
                     PLPEMLAELLGTGSGSKKVAAAYRELLEQAGSEFALLMEKSEKEIEAMHIVGLSGELL
                     ALAIGRMRAGKVSIKPGYDGEYGIIHVFAPGERIQAKAEHSLFFEEKASAEPFCKETQ
                     INNQFEKTLEEQSQESNLAATRTSVPEKDKEDQPNPHEKEKEKAQNSFKPSNSDRNAD
                     SVFRISPEQKAAINHYGGPALVVAGPGTGKTAILALRIARLVGQGIDPSSILAITFTN
                     KAASELRRRIEESMGAEKAAQIKAKTFHSFCLSVLREHAAEASLPQDFAVLDEEERSS
                     FLKSALESDQDGNEQKKASDSLEPSPSDIPGLNLPSEPPTTTSRSRKIKRLASYIEER
                     KRFLLLPGDEIPRLGPCSPAGLAELATELGCPPSNGPKPELELAYARYRILLKQAHAL
                     DFDDLVAGTVRLLAARPALLESYRKRFRAIFVDEYQDVNFAQYALIRLLAPNHEIEEL
                     DLCARELYVIGDPNQAIYGFRGSDRRFIERFIVDYPGAAIYRLLKSFRCTPGIIAAAG
                     RLVNADLSGSGKTIALSRSEFATEASEAEGIAREIDRLIGGTSFFAIDSGVAGSFDFR
                     EAPRPMESHINTSISEKASPDSGDGSNGIDSSDTIDSDTIYIKERPALSSLGESAILI
                     RAATLALPVEKALIDHGIPYRFIGDKPWWEEEPTRSVLIFLRTSIRTDSLDASLLKNP
                     AEAIRFAIDLMKNKKGIKKPKGASLNEPQSSFERLTTQASLYNDLSSFLDDLALGSPQ
                     DGYEASSQCVSLLTIHAAKGLEFDYVFVPCLEDGILPFTIFENEDYRKNQIQEERRLL
                     YVAMTRARIGLYLSWARSRRLFGRKLSLPPSRFLSEIEELVPLVKNNAPGKPKDIQLT
                     LF"
     gene            complement(9560..11911)
                     /gene="feoB"
                     /locus_tag="PK105_05530"
     CDS             complement(9560..11911)
                     /gene="feoB"
                     /locus_tag="PK105_05530"
                     /inference="COORDINATES: protein motif:HMM:NF007105.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HOU61206.1"
                     /translation="MKEKGTTIAVVGNPNCGKTTLFNALTGSDQRVGNWPGVTVEKKE
                     GFLRLGKEKVSLIDLPGIYALFAASEDERIARDYILSGEPGLIVDIVDATNIERNLFL
                     TTQLLDLQIPLIVVVNMVDLAEGRGIYIDYQALSDRLGCPVVGTVASDKRQSAKVLVA
                     IERAWKAKRKPSILVEAPYQAELFAKNWSERLSKVAEALGTNGRWLAFKLLERDEWGV
                     HRVVSSGVATLSEIEAEILKVEKNLAESADIVLAEARYEFIKKVISESVRHIERKRTA
                     TEAIDKVVLNRWLGIPIFLVIMYLLFWITINVGGAFIDFFDGLGGTLFVDGLGHLLDG
                     LGTPTWLRVIIANGIGGGIQTVGTFVPIIFVMFFMLSLLEDSGYMARAAFVMDRFMRW
                     VGLPGRSFVPMLVGFGCNVPAIMATRTLENKRDRYLTIFMNPFMSCGARLPVYALFGA
                     AFFGAASGAMVFSLYLIGILVAIGTGLILKRTLFKGEASFFVMELPQYHKPLMSNVMQ
                     YSGRRLGAFISRAKYIIPIVTILAVLNSLGVDGSFGNEDNPKSVLSQVGRAITPIFEP
                     MGVEKENWPASVGIFTGILAKETVIGTLSSLYSQKAAAIAEENLAAIDAEDLSANGDL
                     DLWDSILAAIGTIPQNLSGIGSSLGDPLGIGMVSGDQEAVAEEVGADTGVYAGLRANF
                     TGGPLQAYAYLLFVLLYLPCVAAFGAMTREMGLKYTLIATAYISVTSWTSATLFYQIS
                     LGHNISWIIFSVLLFAAMIIALKALGKEAKDAVIDPSAKAVGV"
     gene            complement(11908..12144)
                     /locus_tag="PK105_05535"
     CDS             complement(11908..12144)
                     /locus_tag="PK105_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOU61207.1"
                     /translation="MKDKLRDYEVGDSVVILGYTEGDRRYRNKLLSMGLTKGCVIKLV
                     KVAPLGDPVEIEVRGYGLSLRKDEADALIVGGKI"
     gene            complement(12141..12488)
                     /locus_tag="PK105_05540"
     CDS             complement(12141..12488)
                     /locus_tag="PK105_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOU61208.1"
                     /translation="MNRKREHERKDKRALHHLCGECSFSQRSGIALENVLSEARPGEY
                     EFIGILSDRPAFRCRMMSFGLLPGTKIRILEGGRGRPFLVEIRGGKFMLDLRSATMVA
                     IRQIPPVTEGGKQ"
BASE COUNT         3307 a         3133 c         2937 g         3240 t
ORIGIN      
        1 cacaaaccag ggagatcatt cgggaaaacc aggcctttac ctggaatcca tcgattacgg
       61 gagctaaaag cgaagatacg atgcttgcca caaagaatgg tccaattgtc ctctccgaac
      121 cctcgatttt tccagtactc actattgaaa gaaatggcta tgtattcaaa aggccggaca
      181 tcttggaact ataagaagga ggtctctgcc tgcgaagaga aaaaaggaac tgtaagaagg
      241 ggcgcttcgc cctaaaacat tgaataacaa cttttaggag gggactaaag tccccacaac
      301 atatttaagg aggtttccta tgaaagcaaa aaaagccgtg cttttgattt ctgtgctctt
      361 gcttcttagt accttatgcc ttggagcgca gccaaaggaa aaattcccca caagggagat
      421 cgaaatcatg gtgccatggg ctgccggcgg agctactgat ctcatggtgc ggattttctc
      481 accggtgatc cagaaatatc ttggcgtccc gattctcatc accaacaagc caggaggggc
      541 tgcggttcca ggttatgtgg aagctatgac aaaaaagccc gatggctatt acctggtagc
      601 atgggcgacg ccatccataa ccgttacgca tatgcagaag actccctatg atgccaacag
      661 cttcgagccg atcatcaaca tagtcactgc accagtgtgg ttcttagcac cggcttcttc
      721 tccctacaac aacttgaagg atcttgtgga agatgcaaaa aagaggcccg gaaggatcaa
      781 tctaggaaat gccggggcgg gcggaggtac ccatatgata gcactggcct tcgaatcggc
      841 cgtcggggta aagttcaatc atgtaccgta ctcgggagga gcgccagcag ttaccgcagc
      901 tgtcgcgggg caagtcgatg taattacctg tgggccacca gagggcgtgc cgcaactggc
      961 tgccggacag cttaaatgtc tgggtgtttt ctccgaaaaa cggctcgagg aattccccaa
     1021 ctaccctaca gccaaggaac tagggtataa ttttgccatg ggacaatgga gagggcttgc
     1081 ggctcccaag ggaaccgatc ccgaaaggat caagataatt cacgatgcct ttaaggcggc
     1141 aatgtccgat ccggaagtca taaagcttgc tcagcaatcg ggcttattgt tcgattaccg
     1201 caacaccgag gatttcaaaa agcttataca ggaagaagat gcctattata tgaagatcgt
     1261 gaaggaaaac aaactgggga gcaagtactg agaatacatg acaggagagc gggatacatc
     1321 cggccaggcc atgattgtga tgtttcaagt aatcctggca ttatagggct ttgagccgcc
     1381 gcggcgttgg ccggaatgct ctctggtagc attctgtgca cagagattgc tctctatatt
     1441 agcatctcat acacatggtc aagttggagg atttcataag aatggccaag gcagacatag
     1501 tattaggaat aatttttgcg gctctttcta tagtattcta tattggaact ctttcttttc
     1561 cgaaatttag cattggtata aatcccaagg tttatccgct cttcatcatt atctctactt
     1621 ttgcgctctc agtgatgcta atggttcaag gtattattaa gctccgaaaa gaaagaccgc
     1681 cttccacaaa gacatccgca gagaaaggta tctcaaaacc tagctcttgt gatgtaagcc
     1741 ttccgaaggg caagacagct ctaaaactca tcgccttggc cattgttata ctggcctatg
     1801 taattggttt cgaacctcta ggctatataa tcgtaactcc gctcttgatg gcctttacga
     1861 tgtacctctt tggtgaaaga aagccgctcc gaataatcgt tgtatctata ctcgttccgg
     1921 taatcctata ttgggttttt cggactgtgt tcagggtacc cctgccgcgt tctattatct
     1981 ggtaagagga aacctaccat gacagcaata atcaatgcgc ttctctatgt cctcgcaaac
     2041 ttgcatttcc ttataattgg cgtggctgga ggtatcatcg tgggggcgat ccccggcttg
     2101 tccggatcca cgggtataat tctcatgttg ccttttctga tctacataga tccgcccgca
     2161 gctctaatca tgatgagtgg acttttttgc ggttcgatgt ttggtggttc gattgcatct
     2221 atccttgttt cgacacctgg cacaccttct gcggcagcaa cagtgctgga tggctatccg
     2281 ctcgccaaga agggagaagc aggaaaggcg atgggtgttg ccctaatcgc ctcgacattg
     2341 ggtggcatat tttccactct ctgcctaatc tttctttcgc cccagcttgc aaagattgcc
     2401 cttaaattcg ggccacctga atattttgca ctaatgattt tcggcctcac aataatggct
     2461 agtgtctctt cggcgtctct gactaagggt tttctatcgg gcttctttgg acttctgctt
     2521 tcgatggtcg gcttgaatcc cacactgggc accgagcgct ttaccttcgg agttacacga
     2581 cttctcacgg gcttttctat gatgccagta ctcatcggtc tctttgcggt ctccgaagtc
     2641 tttgcccagt tggaacttat gagtgaggga aaaatcgtcc ggctttcaaa aaaacacaag
     2701 atcggatcta tcatgcctac aaaaaaggaa atgaggggtc tatggaaagt gatcttagga
     2761 tcttcggtca tagggaccat aattgggata attcctgcta ctggtggttc tattgcttcc
     2821 tttatggctt acaacgaagc aaagcgattc tcgaaggagc ccgattcctt tggtacaggt
     2881 aatcttgccg ggatagcggc gccagaagcg gctaacaatg caactacggg cggagcaatg
     2941 attcctcttc tcaccttagg aatcccagga gatgtcgtaa cctctgtaat gctgggcgct
     3001 ctaatgatta tcggcgttca gcccggacct cttttgtttt ctcaatcgcc tcagataatc
     3061 accgatctat tcatcggatt cctgcttgca caattcatca tggtggcttt gggttacctc
     3121 agcattcgcg cttccaatct gattctgaga gtgcctctgg gtatcctata tcccacaatt
     3181 ctgtgtctat gccttctggg ggcttattct ctgggcaata gcgtctacga tgtcatgctt
     3241 gcgcttgtat tcggaattgt tggctatttt atgaagaaat atggattttc cgcgccatcg
     3301 gtgatattag gtctcattct tggacctatt gcagaacaag agctcagcag agctctgatc
     3361 atatcacatg gggattggac agtattgatc cgttcgccgc ttgcaatcat gttctatgcc
     3421 tttgcggttg cctcgatatt ctattccttc agaagcttca agaggagcaa aaccaagtaa
     3481 aaatcgaaat ttagagtcta gctctaagaa gccttcgtaa gaagtctccg tcaagagcct
     3541 ccgtaagaaa cttctaagca gcagagctat ggaaggcgag cttgcctagt ccgatgcgcc
     3601 ttttttattc tttgctgatg gcacttgcct cgccaatgtc cgcttgtagc tggcttctaa
     3661 atggctttgc atcgcagcga aagatccatc ggcatcatgg tctgcgatag ctttgtagat
     3721 caatttgtgt tcttcgaaag catgacgatg atcttcgctt gtccttggtg catcctggta
     3781 ggttttctct atcgattcca tgataatagg gatgattcgc attattacgg gattccgggt
     3841 agcctcggca atgctcctat ggaagtcgag ctcagcgttt atctccactt tatgccgata
     3901 gacgacctct tccatggctt ttacactagc cgctattttg gctatgtcat tgctgtcggc
     3961 tttctgagcg gcaagacgag ccgcacccgg ttcgatgaga agccgcgcct cgataatatc
     4021 gagacggagg ttgggagtgg taataaaatc caagcccagg ggatcctgtg caatacccgg
     4081 cacctccgtt acatatgtgc cttttcctcg gcgtatctcg atgatcccct gcgagatgag
     4141 gattttaacg gcctctctta tggtggggcg cgaaacagaa aaaaggctac agagttcgag
     4201 ttcgttgggg agcttctcac caggtttcag tttctcatcg cggatgagtt ccttgatatg
     4261 agaagcgact tcctgagatc gctgtttttt agaagacaga ttgacgtaca taattcctca
     4321 tacccgatag aagtgccaaa aaagcatagt aacctagatt atactgctta aaataaaatc
     4381 aacaagctgt attgacttca gccagatata agatatcatc aattttatga aacagaacga
     4441 aataactagg ccaatcgaac ttttggatga cgaggggcat atcaccgaag agggttgggc
     4501 gcgacgccct ttctggcgct atgacagatc gcgcataaaa gcgccatggt atcgaattaa
     4561 ggaatgggat tactattgtg tgctctcgca gaagcgcggt tacggtattg cgtttacagt
     4621 gtcggatcta ggctatgcgg ggctttcgag catagtgtgg ctagattttg taaagcgtac
     4681 atttacacct gtggatagct tgaaactgct tacgctaggc aagctcggac attcgccagt
     4741 cccagaacga ggagatctgc attataaaga taagaaactc aacatctctt atcttatcgg
     4801 agatggcgaa aggcgtatcg tggctgaaag tccctctttt ggcgaaggga aaggcctcaa
     4861 gtgcgacata aggctcgcac aggatatgtc cgccgacagc atgaatatcg ccacatcttg
     4921 gaaggagaac cggcgagcct tctattacaa tcacaaggta aattgcatgc cagcgctcgg
     4981 cgaagttatc attggcaacg agaccttcaa attcgagccg gaggattcgt ttggtgtctt
     5041 ggactgggga aggggctact ggacatatcg caatcgctgg ttctggggct cggcttctgg
     5101 tctatatgaa ggcgttccta tcggctggaa tctgggatat ggattttccg ataggactcc
     5161 tgcttcggag aacatgattt tctacaatgg cagagcccat aagctcgaag agatagaatt
     5221 tcatatcgat ccggagaatt atctggctcc atggaaaatc accagcagcg acgggcgctt
     5281 cgagatgaat ttcgagcctg tgcttaaccg ccatagcaaa ttcaaggttc tgatgctctc
     5341 ctcgatacag aatcagcttt ttggatatta ttctgggcaa cttatcctcg acgatggacg
     5401 gacattgcat atagacaaga tacttgggct tgcagaagat gttctgaatc aatggtgatt
     5461 taattcccaa cttatcgacg aatttgcccg attacctgac tgcctgcctg aaaagctatc
     5521 ggccgagagg agcttcttta cagcatgaga ataattgccg atcttcatat acactctcga
     5581 ttttctcgcg ccaccagccc caaactgacc ttgccctaca ttgaacgctg ggctcggata
     5641 aagggcataa accttgtagg gaccggagat tgcacacatc cggcttggct caaggagcta
     5701 aatgagcaac ttgagcctac agaaggcgga ttctttaagc tcaaggattc tgtcagggaa
     5761 gaattcgacg caagcaaagc tctgatggaa gggctgcctg attcaaaact atgggctgct
     5821 tggaatgaga ggctgcagcc cgggcggcct tctcaaggtt ctgtgcgctt tgtgctgacc
     5881 ggagaaataa gcaccatcta cacgagagat ggcaaaacca gaaaaatcca tcatatcgta
     5941 atattaccgg atctaaaggc tgcggctgcc tttcaggcgc gactttcacg gataggaaac
     6001 atctcttccg acgggcgacc catacttggt atggattcgc gcgatctctt tactgcactt
     6061 ttggattctg atgagcgttc gattcttata cccgcccata tctggacacc atggttctcc
     6121 gcgctgggag cgcgttcggg cttcgattcc atcgaagagt gctatggtgg tgagcttagc
     6181 tcgcgaattg gtgcaataga gacaggtctg tcttccaatc ccccgatgaa ctgggctgtg
     6241 agctccttgg atgaatttgc cattatatcc aattcggatg ctcattcgcc agagaagcta
     6301 ggccgtgaag cgacaattat cgacatggaa gaatctttcg ctagcctttc cgaggcactc
     6361 acaagaaaag aatcagactc aacggaaaca catagcaacg ctgcaggaca ggccgcagcc
     6421 tcagcaacaa gagcggccat agatgcagtt gcggcggcaa ctacagggac ggtcctggga
     6481 acaatagaat ttttccccca ggaagggaaa tatcactatg acggtcaccg agcctgcaga
     6541 attttcctct cacctcagga aagtgcaagg cttagtgggc gctgtcctgt atgcggcaag
     6601 cccattacac caggggtcat gcgccgtgtt acagagctag ccgatcgccc agtggacgaa
     6661 aatgctccct gccctgctga ctttgaaggc acaaatcgac gaccttactg gcagttaata
     6721 ccccttcctg aaatgctagc agaactactc ggtaccggaa gcggttccaa aaaagtcgcc
     6781 gccgcttacc gcgaactcct agagcaagct ggcagtgagt ttgccttact catggaaaag
     6841 agcgaaaaag aaattgaagc tatgcatatt gttggcctat caggagagct tctcgcgctg
     6901 gccataggac gaatgagagc tggcaaagtc tcgataaagc ctggatacga tggagaatat
     6961 gggataatcc acgtttttgc acccggagaa cgaatccagg caaaagcaga acacagcctt
     7021 ttcttcgaag aaaaggcgtc tgccgagcct ttttgcaaag aaacgcagat caacaatcaa
     7081 ttcgaaaaaa cactagagga acaaagccaa gagagcaatc tagctgcgac cagaacctct
     7141 gtccctgaga aggacaaaga agaccaacca aatcctcacg agaaggaaaa agaaaaagcg
     7201 caaaattctt ttaagccttc taatagtgat cggaacgccg attcagtctt ccggatcagc
     7261 ccagaacaga aagccgctat caatcactat ggaggaccag cccttgttgt agccgggcct
     7321 ggcacaggca agactgccat actcgccctt cgtatcgctc gccttgtagg gcaaggtatc
     7381 gacccgtcat cgattctcgc gatcacattt acaaacaaag ctgcaagcga actccgaagg
     7441 cgcatagaag agtccatggg agctgagaag gcagcgcaga tcaaggcaaa gacattccat
     7501 tctttttgtc tttcagtctt gagagaacat gcggccgagg cctctcttcc gcaggacttc
     7561 gcggtactcg acgaagaaga aagatcttcc tttttaaaat cggcacttga atccgatcaa
     7621 gatggcaacg agcaaaaaaa agccagcgac tcgctagaac catcgccttc cgatattcca
     7681 gggctcaatt tgccttccga gcctcccact acaacttcgc gttcccgcaa aatcaagcgc
     7741 cttgcctcat atatcgaaga gcgcaaacgc tttcttcttc taccaggcga tgagatacca
     7801 cgccttggac cgtgctctcc cgctggtctc gcagaattgg ctaccgagct cggctgcccg
     7861 ccatctaatg ggcccaaacc agagcttgag ctcgcatatg ctcgctatcg catcctgctc
     7921 aagcaggcgc atgccctcga tttcgatgac cttgtggccg gcaccgttcg acttctagca
     7981 gctcggccag ccttgcttga gtcatatcgc aagcgattcc gagccatatt tgtcgacgaa
     8041 tatcaggatg tcaactttgc tcagtacgct ttgatacgac tccttgcgcc taatcacgaa
     8101 attgaagagc tagatctctg cgcgcgggag ctctatgtaa ttggcgaccc aaatcaggct
     8161 atctatggtt ttcggggctc tgacaggcga ttcatcgagc gattcatcgt ggactatcca
     8221 ggcgccgcga tctatcgtct tctaaagagc ttccgctgta ctccgggaat tatcgctgct
     8281 gcaggtcgtc ttgtgaatgc cgatctaagc ggctccggga agactattgc gctttcaaga
     8341 tcggaattcg caaccgaagc gtcggaggca gagggcatag ctcgtgagat agatcgtcta
     8401 ataggaggca ccagcttctt tgcaatagat tctggcgttg cggggtcttt cgattttaga
     8461 gaggctcctc gcccaatgga atctcatata aatacaagca tttcggaaaa agcttcgcca
     8521 gatagcggcg atggctccaa tggcatcgat agctccgata ccatcgattc cgataccatc
     8581 tatataaaag agcgcccagc gctctctagc ctaggggaga gcgcaatcct catccgtgct
     8641 gccacgctcg cgcttcctgt cgaaaaagcc ctcatcgatc atggcatacc ctatcgattt
     8701 atcggagata aaccctggtg ggaagaagag cccactagat cagtgctgat tttcttgcga
     8761 acttcgattc gaacggattc tctagacgcc agtctgctca aaaatccagc agaggcaatt
     8821 cgcttcgcaa tagacctcat gaaaaacaag aaaggcataa agaaacccaa aggagcctct
     8881 ctaaatgaac cccaatcttc ttttgagcgc ctcacaaccc aagcttcgct atataacgac
     8941 ctttcatcct tcctcgacga cctagccctt gggagccccc aggatggtta cgaagctagc
     9001 agccagtgcg tctctctatt gacaatccat gctgcaaaag gcttagagtt cgattatgtt
     9061 ttcgttccct gccttgaaga tggaattctt ccttttacaa ttttcgagaa tgaggattac
     9121 cgcaaaaacc aaatccagga agaacgcagg ctgctatatg tggccatgac tcgagcccgc
     9181 ataggactct atctctcctg ggctcgctcc aggcgccttt tcggacgcaa gctcagcctc
     9241 ccgcccagcc gctttctttc cgaaatcgaa gaacttgtgc cgcttgtcaa gaataatgct
     9301 cctggcaagc caaaggatat tcaactcacg cttttttaga gcgagctctt ttggagagcc
     9361 cgggtatatt cggacaggat cgatccgcta cgcgtgtcgc ggatcgctgc ggtgcaggaa
     9421 tgaaagccga ttgatgattc tttgaatgac aagaggctga tgctcataca gagatctctg
     9481 atttctgatt aaaaaatcaa taagcagcat tccccaatga gtagttataa atgaaaaacc
     9541 ggtcttctta tcaagtttct cataccccga ctgcttttgc cgagggatca ataactgcat
     9601 ctttggcttc tttaccgaga gcctttagcg caatgatcat cgcagcgaac agaagaacag
     9661 agaatattat ccaggaaata ttatggccaa ggctaatctg gtagaatagc gttgcgcttg
     9721 tccaagaagt gacagaaata tatgcggttg ctatgagagt atatttcaat cccatctccc
     9781 tggtcatcgc gccaaaggct gctacacagg gcaaatacag aagaacaaag agaaggtatg
     9841 cataagcctg aagcggacct ccggtgaaat tcgctcttaa acccgcatag acaccagtat
     9901 cggcgcctac ttcctctgct acggcctcct ggtcgccgct taccattccg attcctaggg
     9961 gatcgccaag actgctacca atgcccgata aattttgggg tatagtgcca attgcagcaa
    10021 gaatactgtc ccataaatca agatcaccat tggccgatag atcttcagca tctatggcag
    10081 ccaaattctc ctccgctata gcagcggcct tttggctata aagcgaagac aaggtgccta
    10141 tcaccgtttc tttggccaga atgccggtaa aaatgcctac agacgctggc cagttctctt
    10201 tttcaactcc catgggctcg aaaattggag taatggctct tcctacctgc gagaggacgg
    10261 acttgggatt atcctcgttg ccaaaacttc catcgacacc aagggaattg agaacagcaa
    10321 gaattgtaac gatgggaatt atatatttcg ctcggcttat aaaagcccca agtcttcgac
    10381 ctgaatattg catgacattg ctcatgagcg gcttatggta ctgaggaagt tccatgacga
    10441 aaaaagaagc ttctcctttg aatagagtac gcttcagtat gagcccagtg ccgattgcca
    10501 ccagtattcc gataagatag agagaaaaga ccatagcgcc cgatgctgct ccgaagaaag
    10561 ctgcaccgaa aagagcatag accggcaatc gtgcaccgca ggacatgaaa ggattcatga
    10621 aaatcgttaa gtacctatcg cgcttgttct ctaaggtacg agttgccatg atggctggca
    10681 cattgcaacc gaagcccacc agcattggaa cgaaagatct gcctggcaat cccacccatc
    10741 gcatgaatct gtccattacg aaagccgctc ttgccatgta tcccgaatct tcgagaaggg
    10801 aaagcatgaa aaacatgacg aatatgatag gaacaaatgt gcctactgtt tggatccctc
    10861 ctcctattcc atttgcaatg atgactctga gccaggttgg tgtaccaagt ccatcgagaa
    10921 gatgcccaag gccatccacg aataaagtac ctcccaagcc atcgaaaaaa tctatgaagg
    10981 cgcctccaac attgatcgtt atccagaata gcagatacat aattacaaga aagattggga
    11041 tacccagcca tcggttcaat actactttgt ctattgcttc tgtcgctgtt cttttgcgct
    11101 cgatatggcg cacgctttct gagatgactt tcttgatgaa ctcataccga gcttcagcta
    11161 aaacgatatc tgcagattca gcaaggtttt tctcaacttt caaaatctca gcttcgattt
    11221 cggataatgt tgcgacacca gaggatacca ctcgatgaac cccccattcg tctcgctcaa
    11281 gcagcttgaa tgcaagccag cgaccattcg tgccaagggc ttcggcgacc ttggaaagcc
    11341 tttcgctcca attcttcgca aaaagctcgg cttgatatgg agcctcgacc aatatcgagg
    11401 gctttctctt tgctttccat gctctctcta tagcaacaag cacctttgcg ctttggcgtt
    11461 tgtcgctggc aacagtacca acgacaggac agccaagccg gtcggaaagg gcttgataat
    11521 cgatataaat gcctcgccct tcagccaagt ctaccatatt gaccactacg atcagaggaa
    11581 tctgtagatc tagcaattga gtagtaagaa agaggtttct ctcgatattt gtagcatcca
    11641 cgatatcgac gataaggccc ggctcaccgg agagaatata atctctagct attcgctcgt
    11701 cttcggaagc tgcgaataaa gcataaattc caggtaaatc tattagagac actttctcct
    11761 tgcccagtct tagaaaacct tctttcttct ctactgtcac acctggccaa ttgccgactc
    11821 tctgatcgct tcccgtgagc gcgttaaaga gagttgtctt gccgcagtta ggatttccaa
    11881 ctactgctat ggtagttcct ttctctttca tatttttcct cctactatga gggcatcggc
    11941 ttcatccttt ctcagagaaa ggccataacc tcgtacttca atctctacgg ggtcgcccaa
    12001 tggggctact tttaccagct ttatcacaca tcctttagta agccccatag aaagcaactt
    12061 attcctatag cgacgatcgc cttcggtata acccaatatc accacgctat cgccgacctc
    12121 gtaatctctg agtttgtctt tcattgtttt cctccttcgg taactggcgg tatttgcctt
    12181 atcgctacca tggttgcgct tcttagatcc aacatgaatt tgcctcctct tatttccaca
    12241 aggaaaggcc tccctctacc gccttcaagt atgcgtatct ttgttcctgg aagaaggcca
    12301 aatgacatca ttctgcatcg gaaagcgggt ctgtcggaaa gaatgccaat gaattcatac
    12361 tcaccaggcc ttgcttcaga aagaacattc tcgagcgcaa tccctgatct ttgagaaaaa
    12421 ctacattcgc cacaaagatg atggagcgcc ctcttgtctt ttcgctcatg ctcccgcttc
    12481 cgattcattc ttgcaccgtg aaaaccaaat ctatccattt attgcctttc gaatattgga
    12541 taatctcgat tattaaaatc gccttattga taatgataat cattatcaat ataattgaaa
    12601 cacgacaact agaaact
//
LOCUS       DAOOWY010000055        11427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_5655_length_11427_cov_10.710605, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000055 DAOOWY010000000
VERSION     DAOOWY010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5655_length_11427_cov_10.710605"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /gene="nusG"
                     /locus_tag="PK105_05545"
     CDS             <1..547
                     /gene="nusG"
                     /locus_tag="PK105_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOU61209.1"
                     /translation="WYILHVYSGYENKIEKTIRMLIDSGELSRDIVTDVKVPAEEVSE
                     LKDGKKRTISRKILPGYILVEMDLPEDGWKTTCAIIRNISGVTGFVGSSSNARPQPIT
                     MEEVKRIFQRTGELKGERPVYFRQSFSVGEQVKITQGPFESFSGIIDEVNTEKNKIKV
                     SVQIFGRSTPVEVDMTQVEKI"
     gene            717..1148
                     /gene="rplK"
                     /locus_tag="PK105_05550"
     CDS             717..1148
                     /gene="rplK"
                     /locus_tag="PK105_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOU61210.1"
                     /translation="MAKKKITAIVKLQCPAGKATPAPPVGPALGPHGVSAPMFCQQFN
                     ERTKNMEPGLVIPAIITIYQDKTFTFVLKTPPASVLIKKACGIEKGSSFPQKDKVAKL
                     SKAKLEEIAKQKLPDLNANDLEAAKRIVAGTARSMGVDVEL"
     gene            1150..1836
                     /gene="rplA"
                     /locus_tag="PK105_05555"
     CDS             1150..1836
                     /gene="rplA"
                     /locus_tag="PK105_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOU61211.1"
                     /translation="MKRGKKYIEVSKKVDHTQAYEIGDACALVKELKTAKFDETVEVH
                     IRLNLKKSQSVRDTVVLPHQFRAEKRILVFCKPDRVKEALEAGAAYAGSDEYIEKIKG
                     GWLDFDIAVATPDMMKDVGKLGMVLGRRGLMPNPKTGTVTNDLGATLGELRKGRTEYR
                     TDKLGIIHLPVGKVSMEAEKIAENLSTLVEEVNRKRPSDVKGDFVASIFLTPTMGPSV
                     KVVLNKSEKR"
     gene            1841..2392
                     /gene="rplJ"
                     /locus_tag="PK105_05560"
     CDS             1841..2392
                     /gene="rplJ"
                     /locus_tag="PK105_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOU61212.1"
                     /translation="MAIKASKIQPRKVKAIGMLKEMISGSSDFIFTEYRGLTVEQITT
                     LRRQLREKGVELHVVKNNFARLAFEELGYSHAVEPVLRGPTAVAFIKTDSNEVAKILL
                     DFAKETPALVVKGAMIDRQFMDSDRVETFSKLPGRNQLIAMLMSAMQAPAQNLVYVIN
                     AIPTKLVRVLKAIEEKKANEGVL"
     gene            2521..2907
                     /gene="rplL"
                     /locus_tag="PK105_05565"
     CDS             2521..2907
                     /gene="rplL"
                     /locus_tag="PK105_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOU61213.1"
                     /translation="MAALTKEQIIDAIASMTVLEISELVKAMEEKFGVTAAAPVAVAA
                     PAASAAAAPVEEKTEFTVILKGNVPADKKIAIIKEVRSITGLGLKEAKDLVEAGDKAL
                     KENISKEEADKIKKQIEAAGGTVEIK"
     gene            3111..6605
                     /gene="rpoB"
                     /locus_tag="PK105_05570"
     CDS             3111..6605
                     /gene="rpoB"
                     /locus_tag="PK105_05570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOU61214.1"
                     /translation="MTYSGQKIKRIYIGKEFHDVMDIPDLIDIQLSSYEHFLQRDKLR
                     KGEPLELIGLEEVFRSTFPIESPSGDLVIEYERYSLDEKAIKYSELECKQKTLTYSVP
                     LKAVINLVFKRTGEIRQKEIYLGDIPLMTDRGTFIINGAERVIVSQIHRSPGVIFSHD
                     KGVFSSRIIPYRGSWLEFEIDQKHELIHAKIDRKRKILGTMFLRAIGFHSREEIIAAF
                     YTTKKAELSEDQNNKDALVGKILAKPVYMEIDNEQKRLFRAGDKIHLHEIDELINLGI
                     DSIEIIDFEDDNSLHNEMILNCFDREDVKLVKETPDQDEPTKEDAISAVYSVLRPGEP
                     ISLENAERDLHNMFFTARQYDLGDVGRYKLNKKFDFDIKTNTLTKEDIVATMKHLINI
                     YYGEANIDDIDHLGNRRVRAVGELLANVMKVAFSRMERTAKDRMTGKDLETAKPQDLI
                     TIKPIVAAIKEFFGTSQLSQFMDQVNPLAELTHKRRLNALGPGGLNRERAGFEVRDVH
                     YTHYGRMCPIETPEGPNIGLIVSLATYTTVNEYGFLEAPYRRVRNGIVTDEIEYLSAI
                     DEDKYYIAQASAHIDKSGKFLDQTVSCRHQGDYTMRSPAEIQFMDVSPKQIISVSAAL
                     IPFLEHDDANRALMGSNMQRQAVPLVFTEPPRIGTGMEWKTAYDSGVLVKAKRSGIVD
                     YVDANRIVIRPEKPESPDDTDVYQLIKYQRTNQDTCFNHRPIVKRGEKVLKGQVVADG
                     PATNQGELALGRNILAGFVPWNGYNYEDAILISERVVKEDLFTSIHIKEFILEVRDTK
                     LGPERITRDIPNTSEKMLDNLDGQGVIRIGAKVKSGDILVGKVTPKSDSDTTPEFKLL
                     NSIFGEKAKDVRDSSLRVPHGIEGTVIDVQRMSRANNDDLQPGVEETVKVLVAVKRKL
                     KEGDKMAGRHGNKGVVARVLPIEDMPYLDNGTPLDICLNPLGVPSRMNIGQIMETELG
                     WAASTLNEWYECHVFQSPSQEQIEVKLREAGLPATSKVMVRDGKTGEFFKNPITVGIV
                     YFLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAANTLQ
                     ELLTIKSDDMTGRSKIYEAIVKGEPHTAAGIPEAFNVMVQELRGLALDIKVYDSKGKQ
                     IALTERDEDIIKKQTGAF"
     gene            6740..11089
                     /gene="rpoC"
                     /locus_tag="PK105_05575"
     CDS             6740..11089
                     /gene="rpoC"
                     /locus_tag="PK105_05575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU61215.1"
                     /translation="MRDIQDFDNISIHLASPEMIRAWSYGEVKKPETINYRTLRPERD
                     GLFCERIFGTTKEWECYCGKFKSIRYKGVICDRCGVEVTNSKVRRERMGHIELASPVS
                     HIWFYRSVPSRMGLLLNLPMATLKSVLYYEKYIVIDPGETELKKMQLLTDEEYREAMD
                     RYGGAFTAGMGAEAIRSLLEQIDLDKMSQELRQKMIERGSKSDKRLLKRIELVEAFRS
                     SGNRPEWMILTVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLLQLNA
                     PDIIIFNEKRMLQEAVDALFDNTRRRRAVKGAGNRPLKSLADMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKMWQCGLPTKMALELFKPFIMKKLVEKDVVYNIKKAKMLVES
                     ETPEVFAVLDEVVKEHPVLLNRAPTLHRLGIQAFEPLLVEGKAIKLHPLVCKAYNADF
                     DGDQMAVHIPLTHAAQAECWTLMLSSRNLLDPASGKTIVYPSQDMVLGINFLSRHRPG
                     AKGEGKRFSSTDELLLACEYGSCEYQALVYVAVRKEPVWNSYEKEAEPSKNHFIKTTG
                     GRILLNEALPKEVPYINYAMTDKDIRMLIEHVYRNTSPFITVNMLDTIKEMGFKYATF
                     FGATIGMDDIVIPKEKAEMIEKANKEVEAIQQQYLAGHITQDERYNRVVEVWSKTNEE
                     LTAVMMKTLEKDRDGFNNIYMMANSGARGSKNQIRQLAGMRGLMAKPSGDIIELPIRS
                     NFREGLSVIEFFISTNGARKGLADTALKTADAGYLTRRLVDIAQDVVINEDDCGTING
                     SIYTALKDGDEIVETLAERIIGRFTLDPIRHPITGQIIVGTNEEITEEISEEIEEAGI
                     GEVSVRTVLTCEAKHGVCRKCYGRNLASGRTVDIGEAVGIIAAQSIGQPGTQLTMRTF
                     HIGGAATKAAEENRISLHYPVYIADIEGSYVKTELGRLLFTRRGYIYVNRVFGVFDFT
                     DEDTLLVKDGQRVLKDDSVIKKADGSYIKSPSISYAKVLGNRLLLISQEQKVEIRNGS
                     EMVVSRGQILLANETIATFDPFNDPIISEHDGIVRYEDIITGVTLNEEINAETGNVEK
                     IIADAGNERDTKEPRIIITDEAGNDIETYYLPEGAYVNVEDGESVRAGRMLAKILKES
                     AKAMDITGGLPRVGELFEARKPKSPAVLALVSGTVTFGGTLKGKRVVNIRDSFGKTHK
                     HLVPKNRRLLVRNGDKVVAGEMLCDGVKNPHDILAILGEQECQRYLMDEVQQVYRAQG
                     VTINDKHIGVIIRQMMKKVEIVNPGDTVFIYGQQVDKHKFHEENRRVMEEGGQAAVAR
                     PILLGITRASLKIDSFFAAASFQETTRVLTDAAIKGEVDQLRGLKENVIIGHLIPAGT
                     GMKQYRQLRLFDAENDDLNALVEEVLEKRKQERELAPPAILQDRTSEISFEETTIFDN
                     DEGFSEEDQSLEE"
BASE COUNT         3288 a         2617 c         2954 g         2568 t
ORIGIN      
        1 ctggtatatt ctccatgttt actccggtta tgaaaataag atagagaaga ctatccgaat
       61 gctcatcgat tctggcgagc tttcgcgcga tatagtcaca gatgtaaagg ttcctgctga
      121 agaggtgtca gaactaaaag atggaaagaa gcgcaccata tctcgcaaga ttcttcctgg
      181 atatatcctt gtagagatgg acttgcccga agatggctgg aagacaacct gtgcaataat
      241 acgaaatatc agcggtgtta caggttttgt ggggtcgtct tccaatgcaa ggcctcagcc
      301 catcacaatg gaagaagtca aacgcatatt ccagcgcacg ggcgagttaa aaggcgagcg
      361 tcccgtctac ttccgtcagt cgttctctgt gggcgagcag gtcaaaataa cacaaggacc
      421 ttttgagtcg ttttcgggga ttatcgatga agtgaatacc gaaaaaaaca agatcaaggt
      481 cagtgtacag atttttggaa gatcgacgcc tgtagaagtc gatatgacac aggtcgagaa
      541 aatataggtt ctcatcccgt aagaggatgc ggagatcttt ataaagcggc ataccagcat
      601 ggtgtctgat atgcttctca gataagggat ttttgctcta atgatgggag tctccatggt
      661 atggagacgc tatcccggct atcgagtgcc gggtatacca caacaggaga aaggctatgg
      721 ccaaaaagaa aattactgca atagtcaaat tgcagtgtcc ggctggcaaa gcgacaccgg
      781 ctccacccgt aggacctgcg cttggtcctc atggtgtctc ggcgcccatg ttctgtcaac
      841 agttcaatga gcgtacgaaa aacatggagc ctggattagt aataccagct atcatcacga
      901 tttatcagga taagacattc acatttgtcc tgaaaacgcc tcccgcgagt gtgctaatca
      961 aaaaagcttg cggtattgaa aagggttctt cgtttcccca gaaggacaag gttgcaaagc
     1021 tttcgaaggc aaagctggag gaaatcgcca agcagaaatt gcccgacctc aatgcaaatg
     1081 atctcgaagc cgcgaaacgc atagttgcgg ggaccgcaag gtccatgggc gtggatgtgg
     1141 agttgtgata tgaagcgcgg taagaaatac atcgaagtat ccaagaaagt ggatcatacg
     1201 caggcctatg agattgggga tgcatgtgca ctcgtaaaag aactcaagac agccaagttc
     1261 gatgaaacgg tggaagtgca cataaggcta aaccttaaga agagccaatc tgtgcgcgac
     1321 acagtcgttc tcccccatca attccgagca gagaagagaa tccttgtgtt ttgcaaaccc
     1381 gatcgcgtca aagaagctct cgaggcgggg gctgcctatg cagggagcga tgaatacata
     1441 gaaaaaatca agggtggatg gctagatttc gatatcgccg ttgcgacacc cgatatgatg
     1501 aaagatgtcg gaaaattggg tatggtgctt ggacgccgcg gccttatgcc gaaccctaag
     1561 acaggtacgg ttacaaacga tctgggcgcc acgcttggcg aactgagaaa aggccgcacc
     1621 gaataccgca ccgacaagct cggcattatt cacctacctg tcgggaaggt atccatggag
     1681 gctgagaaaa ttgccgagaa tctaagcacg cttgtagaag aagtaaatcg caagcgtcct
     1741 tctgatgtaa agggcgattt tgttgcttcg atattcttga cgccaaccat ggggccaagc
     1801 gtcaaagtcg tactgaacaa atcggagaag aggtaagact atggcaatta aagcatcgaa
     1861 aattcagcca aggaaagtca aagccatagg catgctcaaa gaaatgatct cgggctcaag
     1921 cgattttatc tttaccgagt acagaggcct aacggtcgaa cagatcacaa ccttacggag
     1981 gcaactgcgc gagaaaggtg tagaactcca tgttgtcaag aacaactttg cgcgtttggc
     2041 ctttgaggag ctcggttatt cgcatgcggt cgagcctgtg ctaagagggc ccaccgcggt
     2101 tgcttttatc aagactgact cgaatgaagt tgccaagatt ttgctcgact ttgccaagga
     2161 aacaccagca ctggttgtaa agggtgcaat gatcgataga caattcatgg attctgatag
     2221 ggttgaaaca ttcagcaagt tgcctggccg caatcagcta atagcaatgc taatgtctgc
     2281 catgcaagca ccagcgcaga acctcgtata tgtcatcaat gcaattccga ccaagttggt
     2341 tcgagttctc aaggcgatag aggaaaagaa ggccaatgaa ggggttcttt gatcgtttat
     2401 ttggcatgct ttttggaaaa agcgagcatc cgtcaggctt cggacgctgc cgttcctgtc
     2461 ggagttcgat agaaggtcca ttcgttgtga tggattacca tgagattagg agaagataat
     2521 atggcagcac tcacgaaaga acaaatcatc gacgcaattg cttcgatgac agtcctcgaa
     2581 atttccgaac tggtaaaagc gatggaagag aagtttggcg taaccgcggc cgcacccgtg
     2641 gcagttgccg ctcccgctgc ttctgctgca gctgctcctg ttgaggagaa gaccgagttt
     2701 acagtgatcc tcaagggcaa tgtgccagct gacaagaaaa tcgctattat taaggaagtc
     2761 cgttcgatca ccggtcttgg cctgaaagag gctaaagatc tggtcgaagc tggagacaag
     2821 gctctcaagg agaacatctc taaagaagaa gcggacaaga tcaagaagca gatcgaagcg
     2881 gctggtggaa cagtcgagat aaaataagtc tgggcttgga gttagagcct tagtcgatga
     2941 aaagaagggg gattgagatc ccccgttttc gggaagcatt ccagccgatt gaatcttgca
     3001 aaaaagagtg cttcccgatg acggaataaa ataaagcacc gatgttcgat atatcggcat
     3061 cggcgcatga acctttgacc cgagcgctaa aggcgcatgg agggtaatga atgacatatt
     3121 caggacagaa gatcaagcgg atttacatag gaaaggaatt ccacgatgta atggatatac
     3181 ccgatctcat cgatattcag ctctcatctt acgagcattt tctccaaaga gataaactcc
     3241 gtaagggaga gcctctcgaa ttgatcggcc ttgaggaggt attccgcagc acttttccta
     3301 tagaaagtcc atccggtgat ctggtgatcg aatatgaacg atattctcta gatgaaaaag
     3361 ccatcaagta ttctgaacta gaatgcaagc agaagacact gacctattcc gtgcctctaa
     3421 aggcagtcat aaaccttgtg ttcaagagga ccggagagat aagacagaaa gagatctact
     3481 tgggcgatat cccgctaatg accgaccgcg gtacatttat catcaatggg gcggaacgag
     3541 tcatcgtttc tcagattcat cgttcgccgg gcgttatttt tagtcatgac aaaggcgtat
     3601 tttcctcacg cataattcca tatagaggaa gttggctcga attcgagatt gaccagaagc
     3661 acgagctcat tcacgccaag atagatcgca agcgaaagat cctgggaacc atgttcttgc
     3721 gcgcaattgg ctttcattcg cgcgaagaga tcatcgcggc attctataca accaagaaag
     3781 cggagctctc ggaagatcag aacaataaag atgctcttgt tggcaaaata cttgcaaaac
     3841 ccgtatatat ggagatcgat aacgagcaaa agcgcctttt ccgagccggc gacaagattc
     3901 atctgcacga aatcgacgaa ctcatcaacc taggcataga ttctatcgaa atcatagatt
     3961 ttgaagacga taattcgctg cacaacgaga tgatcttgaa ctgcttcgat agagaagatg
     4021 tcaagcttgt aaaggaaaca ccggaccagg atgagccgac taaggaagac gcgatctcgg
     4081 cggtttattc tgtgctcaga ccaggtgaac ccatatctct ggagaatgcc gagcgcgatc
     4141 tacataacat gttttttacc gccagacagt acgatctggg cgatgtaggc cgctacaagc
     4201 taaacaaaaa attcgatttc gatatcaaga cgaatacact caccaaggaa gatatcgtcg
     4261 caacgatgaa acacctcata aatatctatt atggggaggc gaatatcgat gatatagatc
     4321 atctaggaaa tcgccgcgta cgcgcagttg gcgagcttct tgcaaatgtc atgaaggtag
     4381 cttttagccg tatggagcgc accgcaaagg acaggatgac cggcaaagac ctcgaaacag
     4441 caaagccaca agacctcatt accatcaagc caatcgtagc ggctatcaaa gaattctttg
     4501 gtacctcgca gctttctcag tttatggacc aggtcaaccc gctggccgag ctaacccaca
     4561 agcgcagact gaacgccctt gggcctggtg gactaaaccg cgaacgcgcg ggcttcgaag
     4621 tgcgcgatgt ccactacacc cattatggac gaatgtgccc catcgagacg ccagaaggac
     4681 ccaacatcgg gttaattgta tcgcttgcaa cctatacgac ggtcaacgag tacggattcc
     4741 ttgaggcacc gtatcgccgc gtaagaaatg gcattgtaac cgacgaaatt gagtaccttt
     4801 ccgctatcga cgaagacaaa tattatatcg cacaggcttc tgctcatatc gacaaaagcg
     4861 gtaaatttct cgaccaaacc gtatcctgcc gccatcaggg cgactacaca atgcgatctc
     4921 cagcggaaat acaattcatg gatgtctcgc ccaagcagat aatatcggta tcggcggctc
     4981 tgattccatt cctcgagcac gatgatgcta accgagctct tatgggttcg aatatgcagc
     5041 gccaggcggt gcctttggtt tttaccgaac ctccgcgcat cggcactggt atggaatgga
     5101 aaacggctta tgattcgggt gttttggtca aggccaaacg ctccggaatc gtcgattatg
     5161 tggacgctaa cagaattgtc attcgacctg aaaaacccga atcgcccgat gatacggatg
     5221 tatatcagct cataaaatat caaaggacca atcaggatac ctgtttcaac cacaggccga
     5281 ttgtaaagcg cggcgagaag gtgctgaaag gccaagtcgt tgcggatggg ccggctacta
     5341 accagggaga gctggcactt ggccgcaaca ttcttgctgg attcgtgccc tggaatggct
     5401 ataactacga ggacgccatc ctgatatccg aacgtgtagt aaaggaagac ctcttcacct
     5461 ctatccatat aaaggaattc attttagagg tgcgcgacac aaagctgggt ccagagcgca
     5521 taacgcgcga cattccaaat acaagcgaaa aaatgctgga taatctagat ggtcaaggcg
     5581 ttattaggat tggcgccaaa gtaaagagcg gagacatttt ggtaggaaaa gtaaccccaa
     5641 aaagcgactc ggatacgact ccagaattca agcttctaaa ctcgatattc ggcgaaaagg
     5701 ccaaagatgt ccgcgattcc tcgctccgcg tgccacatgg catcgagggg acggtcatag
     5761 acgtacagcg catgtcacga gcaaacaacg atgatctcca gccgggcgtc gaagagaccg
     5821 taaaggttct ggtagcagta aaacgaaagc tcaaggaagg cgacaagatg gcaggtcgtc
     5881 atggcaataa gggagtcgtt gcacgcgttc tccctattga ggacatgccc tacctggata
     5941 atggtacgcc gcttgacatc tgcctaaacc cgcttggtgt cccgagccgc atgaatattg
     6001 gccagatcat ggagaccgag ctcggttggg cagccagcac tctaaatgaa tggtatgaat
     6061 gccatgtatt ccaatcgccc tctcaagaac agatcgaagt aaagctaaga gaagcggggt
     6121 tgcctgccac atcgaaggtt atggttcgcg atggcaagac gggagaattc ttcaagaatc
     6181 cgataacagt cggaatcgta tacttcctga aattacacca cctcgtagat gacaagatgc
     6241 atgctcgatc gactggccct tattcgctgg taactcagca gcccctgggc ggcaaagcgc
     6301 aatttggcgg ccagagattg ggcgagatgg aagtgtgggc actcgaggca tatggggcag
     6361 caaacaccct gcaagagctt cttacgatca agtcggacga catgacagga cgctccaaga
     6421 tctacgaggc aatagtcaag ggggaaccac atacggcagc cggaattccc gaggccttca
     6481 atgtcatggt gcaggagctt cgaggcttgg cgctggacat caaggtgtat gactctaagg
     6541 gtaaacaaat tgctttgact gagcgcgatg aagatattat caagaagcag accggagcat
     6601 tctaggatgg cagatgttgc tgttttgcaa tccatgtccg tcagaccttg cacggtcttc
     6661 tatgcgcctt tttgggtacg gggcgctatg gaaacaatgt tcccaggcat gagagatgcc
     6721 tgcaacccga gggggtaaca tgagagatat tcaggatttt gacaatattt ccattcattt
     6781 ggcaagtccg gagatgattc gggcttggtc ttatggagag gtcaagaagc ctgagacgat
     6841 caattacagg accttgcgcc ctgagagaga tggccttttc tgtgaacgaa tctttggtac
     6901 taccaaggaa tgggagtgct attgtggcaa attcaagtct atcagatata agggagtcat
     6961 ctgcgaccgc tgcggtgtcg aagtgaccaa ttccaaggtg cggcgggagc gcatggggca
     7021 tatagagcta gcgagcccgg tatcgcacat ctggttttac cgctccgtgc ccagccgtat
     7081 gggtcttttg ctcaatctgc cgatggcgac actcaaatct gtcctatatt acgaaaaata
     7141 tatcgtaatc gatccaggag agaccgagct caagaagatg cagctgctca ccgacgagga
     7201 atatcgtgag gccatggaca gatatggtgg tgcttttacc gcaggcatgg gggctgaagc
     7261 tattcgatct cttctcgagc agatagacct cgataagatg tctcaggaac tcagacagaa
     7321 gatgatagag cgcggaagca agtccgacaa gcgccttttg aagcgtatcg agctcgtaga
     7381 agcatttaga tcttccggaa atcggcctga atggatgatc ctaaccgtga ttcccgtaat
     7441 tccgccggaa ttaagaccca tggttcagct cgatggtggt cggttcgcca ctagcgacct
     7501 aaacgatctc tatcgtcgag tcataaaccg caataatcgc ctcaagaagc ttttgcagct
     7561 aaacgcgccg gatatcatca tctttaatga gaagcgcatg ctccaggaag ccgtcgatgc
     7621 gctattcgac aacactcgtc gccgcagagc ggtaaaggga gcgggcaata ggccgctcaa
     7681 atccctggca gatatgctaa agggaaaaca ggggcgcttc cgtcagaacc tattgggtaa
     7741 gcgagtcgac tactctggcc gatcggtaat agttgttggg ccagagctca agatgtggca
     7801 gtgcggtctt cctaccaaga tggctctcga gctattcaag cctttcatca tgaaaaaact
     7861 ggtggagaag gatgttgtat acaacataaa gaaagccaaa atgctggtag aatcagagac
     7921 gccagaagtc tttgccgtac tggacgaggt tgtaaaggag catccggtgc tccttaatcg
     7981 cgccccaact ctgcaccggc ttggcatcca ggcttttgag cctcttctcg tagagggcaa
     8041 ggcgataaag ctgcatccgc tggtgtgcaa ggcctataat gccgacttcg atggcgatca
     8101 aatggcggtt catattcctc taacccatgc tgcccaggct gaatgctgga ctcttatgct
     8161 ttcgagtcgc aacctgcttg atccagcaag cggaaagacg attgtctatc cttctcaaga
     8221 catggttctc ggaatcaact ttctaagccg ccatagaccg ggggcaaaag gcgagggcaa
     8281 gcgattttca agcacagatg agctccttct tgcctgcgaa tatggttcat gcgaatatca
     8341 ggctcttgtc tatgtggcag tacgcaagga accagtgtgg aattcctatg aaaaagaagc
     8401 agagccttcc aagaaccact tcataaagac gacgggaggc cgcattcttc taaacgaggc
     8461 tctgccaaaa gaggttcctt acataaacta tgcaatgacc gataaagaca ttcgcatgct
     8521 gatcgagcat gtctatcgga acacgagccc ttttatcacc gtgaatatgc tcgacacaat
     8581 caaagaaatg ggctttaagt atgcgacttt ctttggcgct accatcggca tggatgacat
     8641 cgtcattcca aaagaaaagg ccgaaatgat agagaaggcc aataaggaag ttgaggcaat
     8701 ccagcagcag tatcttgcag gccacataac tcaagatgag cgctacaacc gtgtcgtcga
     8761 ggtgtggtca aagacaaacg aagaactcac tgctgtgatg atgaagacgc tggaaaaaga
     8821 ccgtgatggc ttcaacaata tatacatgat ggctaattca ggggctcgcg gaagcaaaaa
     8881 ccagatccgc cagctagcag gaatgcgtgg gcttatggct aagccatccg gagatataat
     8941 cgagcttcct attcgttcca atttccgtga aggtctttcc gtcatcgaat tcttcatctc
     9001 tacaaatgga gcccgtaaag gtctagcaga cacggctctc aagaccgcgg atgcaggtta
     9061 ccttacaagg cgcctcgtag atatcgcaca ggatgtagta atcaacgagg atgactgtgg
     9121 aaccataaat ggttccatct atacggcact caaagatggc gacgaaatcg tcgaaacact
     9181 agccgagcgc ataatcggtc gctttaccct agaccctatc cgccatccca taactggcca
     9241 gatcatagtg ggaaccaacg aggagattac cgaggagatc tccgaggaga tagaagaagc
     9301 aggaatcgga gaagtctcgg ttcgcaccgt tttgacttgc gaggctaaac atggcgtatg
     9361 tcgcaagtgc tatggccgca accttgccag cggcagaacc gtagatattg gagaagctgt
     9421 tggaataatt gcggcacaat ccataggcca accgggcacc cagctcacca tgcgcacttt
     9481 ccatatcggt ggtgcggcaa caaaagccgc ggaagagaac cgaatttcgc ttcattaccc
     9541 agtatacatc gcagatatcg aaggttctta tgtaaagacg gagcttggtc gtctgctatt
     9601 cactcgccgc ggatatatct atgtgaatcg agtctttggc gttttcgatt ttactgatga
     9661 agatactctc cttgtcaaag acggccagcg cgtcttgaaa gatgattcgg ttatcaagaa
     9721 agccgatggt agctatataa aatccccgag catctcttat gccaaggttc tcggcaacag
     9781 actcttgcta atctctcagg agcagaaggt agagattcga aacggttccg aaatggtggt
     9841 atcgcgcggt cagattcttc ttgctaatga gactatagcg accttcgatc cctttaacga
     9901 cccgataata tccgagcatg atggcattgt tcgctacgaa gacatcataa ccggcgttac
     9961 actgaatgaa gagatcaacg ccgagaccgg caacgtggaa aagatcattg cagatgcagg
    10021 caacgagcgt gataccaaag agcctcgaat aataataacc gacgaggccg gaaacgatat
    10081 cgagacctat tacctccctg aaggcgccta tgtaaacgtc gaggatggcg agagcgttcg
    10141 cgcaggcagg atgctggcaa agattctaaa ggaaagtgcc aaggctatgg atatcacagg
    10201 cggtctacct cgggttggtg agctcttcga agcgagaaaa cccaaatccc cagccgttct
    10261 cgcattggtc tctggaactg ttaccttcgg gggcacactc aaaggcaagc gcgtagtcaa
    10321 tatccgcgat tcctttggaa aaacccataa gcacctcgtg ccaaagaatc gacgactttt
    10381 ggtgcgcaat ggggataaag tggtcgcagg cgagatgctc tgcgatgggg taaagaatcc
    10441 acatgatatc cttgcgattt tgggtgaaca ggaatgtcag cgctacctca tggatgaagt
    10501 gcagcaagtc taccgtgctc aaggcgtaac catcaacgat aaacatatag gagtcatcat
    10561 ccgacaaatg atgaagaagg tcgaaatagt aaatccaggt gacacggtat ttatttatgg
    10621 ccagcaagtg gataagcata aatttcatga ggaaaacaga cgggtgatgg aagaaggcgg
    10681 acaggctgca gtagcgcgcc cgattctgct tggtataacg cgtgcatctc taaagatcga
    10741 ttccttcttt gccgcggcgt ctttccagga gacaacaaga gttctgacag atgcagccat
    10801 aaagggagag gtggatcaac tgcgagggct caaggagaat gttatcatag ggcatcttat
    10861 accagcggga accggaatga aacaataccg tcaactgagg cttttcgatg ctgaaaatga
    10921 tgatctcaac gccttggtag aggaagtgct cgaaaagcga aagcaggaac gcgaactagc
    10981 tccacctgct atattgcaag atcgtacaag cgagataagc tttgaggaaa cgacgatctt
    11041 cgacaatgac gaaggattca gcgaagagga ccaatccttg gaggagtaag atcccaaggg
    11101 tttttgagca tgcccaattg tataacttta tggtacaggc attgctctgt atgtgcttga
    11161 tatggccact gcgatttaac ttcggctaga atatagtttt taggcgatgg cttattatcg
    11221 gccagaaaac tttttaggca attcgaggcc ttgacatgtg atgggtgaga aaggtattct
    11281 acaccgctgc taaaacagac accgcagttt ttgcagaccg gtaatttaaa ttatattgaa
    11341 gaaaaccgcg cgcgatgctg acgcgagcgg atgaaaggga gtctagagct tatatgccga
    11401 cgattaacca gctaatccga aatggaa
//
LOCUS       DAOOWY010000056        11425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_5658_length_11425_cov_17.118382, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000056 DAOOWY010000000
VERSION     DAOOWY010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11425
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5658_length_11425_cov_17.118382"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            952..1980
                     /locus_tag="PK105_05580"
     CDS             952..1980
                     /locus_tag="PK105_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU61216.1"
                     /translation="MNKQKISIHEVARLAGVSKSTVSRVISEKGGSVSQETAKRVHTA
                     IDQLGYRKNIVAAGLRTKTTYMVLLMIPDVANPFWSEIARAVQDSLEPEGYSVVIGST
                     DFSEEREARYYELARMSRFDGVILNSVTDNIDLIKNLGIPSVLIGERTAAQHIDTVGT
                     DTLQATKIGMEYLYETGHRKIALATSDHGSERYLSLRARAYKDFLHEKALIFDPSLLF
                     SVNLNEEGGKELARKLLTINNWKNQIDSLFCGNDIVAISAMNELRKFGIIAGVDISII
                     GMDDIPASSQTYPPLTTVRKPRQLIGVAAAHLLLKRIANPQDSTEKHLFPGELVIRDS
                     VINRRKGK"
     gene            1977..2972
                     /locus_tag="PK105_05585"
     CDS             1977..2972
                     /locus_tag="PK105_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU61217.1"
                     /translation="MKSRADILAQREIFESRAIGAFIGLAVGDAVGDLGRNQEYRNRY
                     GLITRLFEDGKSTDDTEFGVLTARMLLDAKANLTPQKTANAWRHYILERGGAKKRAGR
                     PLYGAIENLSRGMEPPLSGRFNVMNIDDGAAMRAIPHGILFAGNPSRAAASAVADACV
                     SHDADGIWAAQAVAAAIAVAIADGTTEEIVHTARSMLPLGSWIAHAMDIAMSICERAQ
                     NIEDAYEDLHIKLWTPEHATAAEAIPQVFAIFKLCNGEPHHSLLWSANFGRDADTISG
                     LVCSFSGAMHGDTAFPNEWIERLRKPSGTCLEFAMEEDIVQLAKDLVALSCSLEE"
     gene            2977..3867
                     /locus_tag="PK105_05590"
     CDS             2977..3867
                     /locus_tag="PK105_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU61218.1"
                     /translation="MHSLSKKKDWGLVLLLAPALIYFLVYHIIPILGMSMAFQDYRII
                     GKSPFVGLKHFRILFSSPAFFQVLRNTLIISFMKIILYFPLPIIFALMLNEVRSGFIR
                     KFIQSTTYLPHFLSWVVIAGIWIAFLSPTNGGFNQLRGFFGLPPHDYMTDKSAIRWVL
                     FASEAWRSMGWDSIIYLAALIAISPSLYESADLDGASTLQKMRFITLPELLPTMVTVF
                     ILNMGFFLNAGFDQVFNMMNDSVISVIDILDTYVYRIGLVNMQYSFSTAASLFKGVIG
                     LMLIVSAHCGSKKITGKGLW"
     gene            3861..4778
                     /locus_tag="PK105_05595"
     CDS             3861..4778
                     /locus_tag="PK105_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU61219.1"
                     /translation="MVKDKYPPKKVRIPRAELFSRWCFTILLLLASILVLVPLLNLLA
                     LSLSDPSRVSEVRGLTIVPKGFSLINYKVLYANPLFMRSILNSVFITIVGTALNLIVT
                     SMAAFALTRPKLPFKRFFMVLTIVIMILEPPLVAEYLVIKKIGLMGSLWAVILYKTVN
                     VYYLIIMMRYFEEIPSSLIEAAEVDGATNMTILRNIVFPLSKSAFATIGLFYGVFHWN
                     EYFRASIYLTDPMKYPLQLILRQFVVLDDTASLIGSGALYSYNEAARLSYNALQASTI
                     IVAILPVLIIYPLVLKYYTRGVMEGGVKE"
     gene            4788..6212
                     /locus_tag="PK105_05600"
     CDS             4788..6212
                     /locus_tag="PK105_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU61220.1"
                     /translation="MKKNKSMLMFAVAIAILGCAVAGAQPKVRLVLKDLDTDPNTPQF
                     IQLIEKGMAAAGTPVKIEVVKVPSGSYAEKVSLMITSGDIPDLIYFQGSDEKIAMQGL
                     LEDLRPYVNKSKYIKNVLDPHSKTRLENYPYLVWVAPPRVRVPVIRKDWYDSLPSAKA
                     LVAKPTIDAYYALFKDLVQKKGAQYGISITGATNGAEELDSIFDAAFGITSTWMKGSD
                     GQWVYCRVTPNEKEKLTFYAKLYKEGLLDPEYLTKKWDTKEQAFYEGKAGMVAGVAPS
                     SVDVYANKMMKTQNTTIVPLPPPTGKSQGLGPFVDVIKESRGFAIPVTSKVKDVAFEV
                     LDFMWGPAGLKIAKLGIPGVHYIEEPNRYVLTDRYPEWVNGWFGDSFNGFNPDKPLSR
                     PIMTDAAVEAGKLAVMYMMPDKTFLIPEQYITNWDAMSNLYKEYEADIITGKKPISAF
                     DEFAQKWNRAGGTELTKYAQTVLK"
     gene            6363..8555
                     /locus_tag="PK105_05605"
     CDS             6363..8555
                     /locus_tag="PK105_05605"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU61221.1"
                     /translation="MASFIPDDYVERVYAGLLGKNIGIRLGAPVEPAIWTYQRIGEIY
                     GDITSYLKAYRNFAADDDANGPVYFVRPFLEKVEEMLKHEPQEARLGDMQLEAQEEGR
                     AWLNYAREGVGMFWWGGYGRSTEHTAYLNLKNGIPAPESGSVRRNGKVVAEQIGGQIF
                     VDTWGLIWPGNPKKASEFAARAASVSHDGEGLNGARFIAASIAAAFDAADVLSIVDTG
                     LGLIPQDSAYASVVQAVKQFHSLYPADWRQCQEYLISEWGYDRWPGLCHIIPNAGVCA
                     LALLYGEGSFARTVEIATMCGWDTDCNAGNVGTIAGVLYGLDSLPEHYRKPINDSIVL
                     SGLPGVLNVLDIPTFADRLARVGYAMSGESIPNYLVESLWKGDGEQALCFDFQLPGST
                     HGMRASDAIRFATGHREIEHNGINRGALEFRFERLTREESGTCYFKPYYRRSDFDDER
                     YSPVFSPLARPGQHVDISFCLEQWEGQRIGIEGYVRDTWSRKEIITTPTLFFDMGKDM
                     TGQPKEEYRVSFDIPSMQGSFPDEVGLKVTSFSGKGKRDSGRLFVLQFTLSGKASYDI
                     DFSKQTIEFGSVTPFSHEGGTWSIENNRLHLMTPTRAAAYTGGYRVANQKVEATVTPL
                     AGDSHMLLVRAKGAQQAYWGGIDDKGKLAVYRQNFGFEKLAETDFAWDHDTDIQMTLS
                     AIGDRISVRIDDEKLLEVRDNELRSGMVGCGSLRGSRTLWGPFAIEEL"
     gene            8571..9818
                     /locus_tag="PK105_05610"
     CDS             8571..9818
                     /locus_tag="PK105_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOU61222.1"
                     /translation="MSNSEQSEAGFLSKSDCIVRYEPSQEPLSIEVRSSVRALFETQI
                     RQCVLRIFQEFDVTKGRIAIEDDGALDLVISARVEAVLRQAGYLSQMKTMSPEKGKYG
                     VSLVSSNGAGFTQSSRRVRLYMPGDQPHFAVNADFYGADILIFDLEDSVAQSRKCEAR
                     ILVRRFLENSFLFRKSARAVRINPLSTEESMLDLAEIVPAKPDIVLLPKCETADDIKR
                     LDALLSEHENRCGTMPGGIQIMPIIETAKGVLAARGIACASKRTSGLCFGYEDFSRDI
                     QSSSDILGKALLMHSGSMHQIDSLLARETIVLAARAAGIEPYDSSYGDIDNTEGLLMS
                     CYEARALGFAGKSVIHPSQIPYVRKAFMPTAEELHQAQAIVAAYEESFSTGKGAIVVD
                     GQMVDIPVVEKARRIIEEFDYGE"
     gene            9808..11388
                     /gene="citF"
                     /locus_tag="PK105_05615"
     CDS             9808..11388
                     /gene="citF"
                     /locus_tag="PK105_05615"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOU61223.1"
                     /translation="MGNDTTWTDNALGRKVPSRIAGKPCRSFLGAFIDSTQAKHEKTK
                     NGQLRRSSGMTQRQKPNKLVASWDELFERLNIQDGATISFHHHLRNGDRIVNEVVGRL
                     AAKGVCDLTLAPSALFPVHNALLPYIRSGVITKIEGSMNGTIGAACSRGEIPKTCVLR
                     SHGGRQRAIQEGDLPIDVAFIAAPCADSFGNANGLYGPSACGPLGYAKPDSEYAHQVV
                     VITDNLVPYPCSSWSIFGGNVDYVLQVESIGDSSQIVSGTTLLTKSPTQLVIARLAAR
                     FIEAAGIMQEGFSFQAGAGSISLATIVFLAERMRKAGVKASFAHGGATKVLVDLLKEG
                     LLGSIVDLQSFDLDAVRSCAENARHIESDPYSSYGPYARGCVANMLDVAVLGATEIDL
                     DFNVNVVTHSDGMLLHGIGGHSDVCSSAGCTIITAPSFRKRIPIIKEQVTTITCPGEA
                     VDVLITERGISINPNREDLIWRAKKFDLPCVDIAQQMNDVYRITGKPEKPRFTELPVA
                     LVEWRDGSIIDTVWQMAE"
BASE COUNT         3139 a         2421 c         2767 g         3098 t
ORIGIN      
        1 ctacttttcg cgagccaaga atcgaagcaa gtcacgatcc aattcgtaca tccgaagatt
       61 ccattgcata gagtaaatat cgcacactat cataaaactg tagataaact gaccaaatta
      121 tgccaaattt agtcagtttg atgacaattt tatagaattg ccaattcaag tctgaagtac
      181 aaaaaggata aatgtagaaa aatggcatta gagaatttgg taggatgtga atatcacaca
      241 gacatccata ccggaagtca caaatgacat gtcagttttt gcaagtcatg agcaagatca
      301 gtttcaggtt ctgatctcgg gcaaaccata aaagtgaggt atggctagga cacttgagga
      361 acatgtcttt tatagctaca gagagatcgt aaggaaccag aaagagggct accatttcgc
      421 ccatcatgca tacactacag cacaacagcg tcgactaaaa ccaaccttca gtataaaacg
      481 agcgaatata gagttaatgc ataaggttta gtaaaggatt ctcaacgagc actcttccat
      541 caggggaata atgaacatcg aaaacgccag tcagacaaat ataaacgagt ggttatatac
      601 aatcggtgct ttattgatga atgtgctcaa attgtaaatt tgaagaatag cttcggtgac
      661 tgagaggaga taatatcgag aaaagttttc tttaatctcc cttttcaacc tcagatttaa
      721 agtagtctca cttaaagagc tttaaaagag ctcagattgt ttgttatctt aaaacattca
      781 ttaaacttga gctttttatt ttgattctaa tatatccaaa aattcaaaaa atattgaatg
      841 cctcatttct ttcttataat tagcttagta ctataaatat agggaacgtt cccaattatg
      901 acttgcatct tcagaaaggt gtgttataat actaatggga acgataccaa tatgaataag
      961 caaaagatta gtattcatga agtggcgcgt ctagcagggg tctcgaaaag cacagtctct
     1021 agagtgattt cggagaaggg cggatctgtc agccaagaga cagccaagcg tgtacatacg
     1081 gcaatcgatc aacttgggta tcgcaagaat atcgtagcag caggcttaag gacaaagaca
     1141 acatatatgg tcctgctcat gattccagat gtagcaaatc cattctggtc agagattgcg
     1201 agagcggtcc aagattcgct cgaaccagaa ggttattcgg tggttatcgg tagcactgat
     1261 tttagtgaag agcgagaagc acgttactat gagctcgctc gaatgtctcg atttgatggg
     1321 gtcattctta atagcgttac tgataatatc gatcttatca agaatctggg tataccctca
     1381 gtcctaattg gtgagcgaac tgctgcacag catattgata cggtagggac cgataccctt
     1441 caagcaacca agattggcat ggaatacctc tatgaaacag gtcatagaaa aatagccctt
     1501 gcaactagtg atcatggtag cgagcgctat ctttctctta gagcacgagc atataaagat
     1561 tttcttcacg aaaaagctct tatttttgat ccctctcttc tattctctgt gaatttgaat
     1621 gaagaaggtg gtaaggagct agcaagaaaa ttgctcacca tcaataactg gaaaaatcaa
     1681 atcgacagct tgttctgtgg caatgacatt gttgcaattt ctgcaatgaa tgagctcagg
     1741 aagtttggca ttattgcggg agtggatatt tcgatcatag gtatggatga tatccctgca
     1801 tcttcccaga catatcctcc gctgacaacc gtacgcaagc ctcgccaact cattggtgtt
     1861 gctgctgcac atttattgtt aaaacggatc gcgaatccgc aggactcaac agaaaaacat
     1921 cttttccctg gtgaacttgt gatcagagat agcgtaataa atagaaggaa aggaaaatga
     1981 aatcacgagc agacattctt gcacaacgag agatatttga atcaagagct attggagctt
     2041 ttattgggct ggcagtcgga gacgcggttg gcgatcttgg gagaaatcag gaatatcgta
     2101 atcgatatgg tcttattact cgtctgtttg aagatggaaa aagcaccgat gataccgaat
     2161 tcggtgtact tactgcacgt atgctactcg atgccaaagc aaatctcaca ccccagaaaa
     2221 ctgcaaatgc atggcgccac tacattcttg aaagaggtgg agcaaagaag agagcagggc
     2281 gaccgcttta tggtgcaata gagaatctca gtcgcggtat ggaaccgcct ctatctggca
     2341 gattcaatgt aatgaatatc gatgatggtg cagcgatgcg agcaatacca catggcattc
     2401 tctttgcagg aaatccttcg cgcgctgctg cttccgcagt agctgatgca tgtgttagtc
     2461 acgatgccga tggtatttgg gcagcgcaag ctgttgctgc agcgatagcg gtagccattg
     2521 cagatggaac cacagaagaa attgtacata ctgcgcgatc gatgttgcca cttgggagct
     2581 ggattgcgca tgcaatggac attgccatga gcatctgcga gcgcgctcaa aatatagaag
     2641 atgcatatga ggacttgcat ataaagcttt ggactccgga gcatgcaacc gctgctgaag
     2701 caattcctca agtgtttgca attttcaagc tttgcaatgg tgagccccac catagtcttc
     2761 tttggagcgc aaattttgga agggatgcag atacaatttc tggtctggtt tgctcattct
     2821 caggcgcgat gcatggcgat accgctttcc caaatgaatg gattgaaaga ttgagaaaac
     2881 cgtctggaac ctgtctcgaa tttgccatgg aagaagatat tgtgcagctc gcaaaggatt
     2941 tagttgcttt atcttgcagt ctagaggaat aagagtatgc attcgctatc aaagaaaaaa
     3001 gattggggcc ttgtactttt gcttgctccc gcactgattt atttccttgt ataccacatt
     3061 attcccattt tggggatgtc aatggctttt caggattatc gtattattgg caaaagccct
     3121 tttgttggac ttaagcattt tcgcatactt ttttcgtcac cggcattctt ccaggtcctt
     3181 cgcaacaccc tcatcatcag ctttatgaaa attatattgt atttcccctt acctataata
     3241 tttgcactta tgctgaatga ggtccgttca ggatttattc gcaaatttat tcagtccacg
     3301 acctatttac cccactttct ctcttgggtt gttattgctg gtatatggat agcttttctt
     3361 tcacctacaa atggtgggtt caatcaactt agaggatttt ttgggcttcc gccacacgat
     3421 tacatgacag acaagtctgc tatccgatgg gtgctgtttg cttctgaggc atggcgcagc
     3481 atgggatggg actcgatcat atacctagct gcattgattg cgatcagccc aagtctctat
     3541 gagtctgctg atctagatgg tgcaagtact ttgcagaaaa tgcgttttat tacacttcct
     3601 gagctccttc caacaatggt gactgtcttt attctcaata tgggcttttt tttgaatgca
     3661 gggttcgatc aggttttcaa tatgatgaat gattctgtaa tcagtgtgat agatatcctt
     3721 gatacctatg tgtacagaat tggccttgtc aatatgcaat attcattttc aacagcagcg
     3781 agtcttttta agggcgttat cggccttatg ttaattgtgt ctgcacattg tggatcaaaa
     3841 aaaataactg ggaaaggttt atggtaaaag ataaatatcc ccctaaaaaa gtgcgaatac
     3901 ctcgagcaga gctcttttcg agatggtgct ttacaatcct attgctattg gcatctatct
     3961 tggtgcttgt tccgttgctc aatttgcttg cgctttctct ttctgatcct tcaagagtct
     4021 cagaagttag aggacttaca atagtgccta agggcttttc gcttatcaac tataaggtac
     4081 tctacgcaaa cccacttttt atgcgcagta ttttaaattc agtctttatc accattgtag
     4141 gaacagcgct gaatcttatt gtgacttcca tggcagcttt tgcattgaca agacctaaac
     4201 tgccatttaa gcgctttttc atggtgctta ctattgtcat tatgattctc gaacctcctc
     4261 ttgttgcaga atatcttgtt atcaaaaaaa ttgggctaat gggcagctta tgggcagtta
     4321 tcctctataa aactgtcaat gtgtattatc tcattattat gatgcgatac tttgaggaaa
     4381 ttccttcttc tcttatagaa gctgcagaag tcgatggtgc aaccaatatg acaatcctaa
     4441 gaaatattgt atttccatta tcaaaatctg catttgcaac aataggactt ttctacggag
     4501 ttttccattg gaacgaatat tttcgcgcaa gcatatatct caccgatcca atgaaatatc
     4561 ctcttcagct tattctacgg caatttgtag tacttgatga tactgcgtcg ttaatcggtt
     4621 cgggagcact atatagttac aacgaagctg caaggcttag ctacaatgca ctacaagcga
     4681 gtaccatcat tgtagcaatt ctaccggttc tcattatcta ccctctagtc ctcaaatatt
     4741 acacacgagg agtaatggag gggggagtaa aagaatagga ggttcttatg aaaaagaaca
     4801 aatctatgtt aatgtttgct gttgcaatag caattctcgg atgtgcagtt gcgggagcac
     4861 aaccaaaggt tcgtttggtc cttaaagacc tcgatacaga tccaaataca ccacagttca
     4921 ttcaacttat cgaaaagggc atggctgcag cgggcacgcc tgtgaagatc gaagtagtca
     4981 aggtaccatc tggtagctat gcggagaagg tgagcctaat gatcacgtcg ggagatattc
     5041 cagacctcat ttatttccaa ggcagcgatg agaaaattgc catgcagggc ttgcttgaag
     5101 atttaagacc atacgtgaac aagtcgaagt acataaagaa tgtactcgat cctcatagta
     5161 aaacaaggct tgaaaattat ccttaccttg tatgggttgc tcctccacgc gtacgcgtac
     5221 cagtgataag aaaagattgg tacgactcgc tgccttctgc aaaagcgctc gttgcaaaac
     5281 ctactataga tgcctattat gcgcttttca aagatctcgt acaaaagaag ggtgcacagt
     5341 atggcatttc tataacaggc gcaaccaatg gtgcagaaga actcgattcc atttttgatg
     5401 cagcctttgg tatcacttct acttggatga aggggagcga tggtcagtgg gtttattgta
     5461 gagtaacacc caatgaaaaa gaaaagctta cattttatgc aaagctttat aaggaaggtc
     5521 tacttgatcc ggaatatctg accaagaaat gggacacaaa ggagcaagcg ttttatgaag
     5581 ggaaggcagg aatggtcgct ggtgtcgcgc catcaagcgt tgatgtctat gcaaataaga
     5641 tgatgaaaac ccaaaataca acgatagttc ctctccctcc cccgaccggt aagtcgcaag
     5701 gtcttgggcc atttgtggat gtgattaagg aatcccgtgg ttttgcaata ccagttactt
     5761 caaaggtaaa agatgtagcg tttgaagttt tggactttat gtggggtccc gctggtctta
     5821 agattgctaa gcttggtatt ccaggtgtcc attatattga ggagccaaat cggtatgtat
     5881 tgacagacag atatcctgaa tgggtgaatg gctggtttgg cgattcattc aatggtttta
     5941 atcctgataa acctctttcc cgtcctatta tgaccgatgc tgcagtagaa gcaggaaagc
     6001 tcgcggtcat gtatatgatg cctgacaaaa cattccttat tccagaacaa tatattacaa
     6061 attgggatgc gatgagcaat ctctacaagg aatatgaagc ggatatcatt actggcaaaa
     6121 agccaatctc agcattcgat gagtttgctc aaaaatggaa tcgagctggc ggtactgaat
     6181 tgacgaagta tgcccagact gtgctgaaat agcacatcgt catgtccgat ataatgaaga
     6241 atcgtttaac agaattgaga gtatgagttt tggggcacag gcatcgccgc gattgagtag
     6301 caagagccaa aaagcacgaa gatgctctgt gccgctatta aataaggcag gtatctacat
     6361 ttatggcctc gtttatccca gatgattatg ttgagcgcgt ttacgcaggt ttgcttggta
     6421 agaatatcgg gattcgtctt ggtgcgcctg ttgaaccggc aatttggaca taccagcgta
     6481 tcggtgaaat ttatggcgac attacatcgt acctgaaggc ttatcggaat tttgccgccg
     6541 atgacgatgc aaatgggccg gtatattttg ttcgcccctt cctggaaaag gtagaagaga
     6601 tgctaaagca tgaaccgcag gaagctcgac taggcgacat gcagctcgaa gcgcaagaag
     6661 aaggaagagc gtggctcaat tatgcccgcg aaggcgttgg aatgttttgg tggggaggat
     6721 atgggcgcag taccgaacac accgcctatc taaatctcaa gaacggaatc cctgcccctg
     6781 aatctggatc ggtacgcagg aacggaaagg tcgttgccga acaaatcggt ggccaaattt
     6841 ttgtagacac ctggggtttg atctggcctg gtaatccaaa gaaggcctcg gaatttgccg
     6901 cgcgcgcagc ctcggtctct cacgacggag aaggcctgaa tggtgcccgt tttattgcag
     6961 cgagtattgc cgccgctttt gatgcagctg atgtgctctc tattgtggac actggccttg
     7021 gccttatacc gcaagactcg gcctatgctt cggttgttca agccgtaaaa caattccatt
     7081 ccttgtaccc ggcagattgg cgacaatgtc aagaatatct catctccgaa tggggatacg
     7141 atcgatggcc ggggctctgt cacataattc ccaacgcagg agtttgtgct cttgcacttc
     7201 tttatggcga gggtagtttt gcgcgcaccg ttgaaatcgc gacgatgtgc ggttgggata
     7261 cggactgtaa tgcaggtaat gtgggtacta ttgcaggagt gctttacggc ctcgattcgt
     7321 tgcctgaaca ttatcgtaag ccaataaacg atagcatcgt gctgtctggg ctgcctggtg
     7381 ttttgaatgt tctcgatatt cctacatttg cagatcggct cgcaagggtt ggctatgcca
     7441 tgtccggaga gagcatcccg aattaccttg tggagtcgtt gtggaagggc gatggcgagc
     7501 aagcgctttg ctttgatttc cagcttcctg gttcgactca tggcatgcga gcctccgatg
     7561 caattcggtt tgcaactggg catcgagaga tagaacacaa tggtataaac cgaggggcct
     7621 tggaatttcg ctttgaacgg cttacacgcg aagaaagtgg aacttgttat ttcaagccgt
     7681 attatagacg atccgatttt gatgacgaac gttacagccc tgttttttct ccgctggcaa
     7741 ggccaggtca gcacgtagat ataagttttt gcctcgaaca atgggaaggt caacgtattg
     7801 gaatagaggg ctatgtgcgc gacacctggt ctcgcaaaga aatcatcacg acgcctactc
     7861 tctttttcga tatgggaaaa gatatgactg gccaaccgaa agaggaatat agagtttctt
     7921 tcgatattcc gtcgatgcaa gggtccttcc ctgacgaagt tggtcttaag gtcacgagtt
     7981 tttcgggcaa gggtaagcgt gacagtggga ggttattcgt tctccaattt accttgagtg
     8041 gaaaagcaag ctacgacatc gatttttcga agcagacgat agagtttggt tctgtgacgc
     8101 cgttttctca tgaaggcggg acatggtcaa ttgagaacaa taggctccat cttatgacgc
     8161 ctacaagagc agccgcgtat acaggcggtt acagagtggc aaaccaaaag gttgaagcaa
     8221 cggtcactcc tcttgcaggg gattctcaca tgctattggt aagggcaaag ggcgcacaac
     8281 aggcgtattg gggcggcatc gacgataaag ggaaacttgc agtttatcgg caaaactttg
     8341 gatttgagaa gctggccgag actgattttg catgggatca cgatacggac atccaaatga
     8401 cgctgtcggc gataggcgac agaatctccg ttcgtatcga cgacgaaaaa ctgctagaag
     8461 tgcgcgataa tgaattgcga agcggaatgg tcgggtgcgg tagtctgagg ggatcgcgaa
     8521 ccctctgggg tccgtttgcg attgaagaac tatgagtaat tcaggacaat atgagtaatt
     8581 cagaacagtc cgaagcgggc tttctttcaa aatcggattg tatcgttcgg tacgaacctt
     8641 cccaagaacc tttgtcgatt gaagttcgtt cgagtgttcg ggcacttttt gagacgcaaa
     8701 tcagacagtg cgtcttaaga atattccaag aattcgacgt aacaaaagga agaattgcga
     8761 tagaagacga tggtgctctc gaccttgtca taagcgcaag agttgaggcg gtgcttcgac
     8821 aagccgggta cctgtcgcaa atgaaaacaa tgtcacctga gaagggaaaa tatggcgttt
     8881 ctcttgtctc ctcgaatggt gccggtttta ctcaatcgag taggcgcgtg cggttgtaca
     8941 tgcctggaga tcaaccgcat tttgcggtaa atgccgattt ctatggtgcg gacatcttga
     9001 tattcgatct cgaggattcg gtggcgcaat ctaggaagtg cgaagcccgc atacttgtac
     9061 ggcgattcct ggagaattct tttctcttta ggaagagcgc acgggctgta aggataaatc
     9121 cactatcaac cgaagaatcg atgctcgatc tcgcggagat tgtccctgca aagcctgata
     9181 ttgttctgtt gccaaagtgc gaaacggccg atgatataaa gcgcctagat gcgctccttt
     9241 ctgagcacga gaatcggtgc ggaacaatgc ctggcggcat tcagattatg ccgatcatcg
     9301 agaccgcaaa aggggtgctt gcagccaggg gaattgcctg tgcatcaaaa cgaactagtg
     9361 ggctttgttt tggttatgaa gatttttcgc gcgacatcca atccagtagc gacatactag
     9421 ggaaggcctt attgatgcac tccggttcta tgcatcaaat agattcgctt ctcgcgcggg
     9481 aaacaatcgt gctcgctgca cgggcagcag gtatagagcc ctacgattcc tcttacggtg
     9541 acatagacaa caccgaaggg cttcttatgt cttgctacga ggcgcgagca cttggttttg
     9601 ccggaaaatc ggtcatccat ccttcgcaaa ttccttatgt aagaaaggct tttatgccca
     9661 ccgccgaaga gttacatcag gcacaggcta tcgtggcggc atacgaggag tcgttctcga
     9721 ccggcaaggg agcgatcgtc gtcgatgggc aaatggtcga cattcctgtc gtggaaaaag
     9781 caagacgcat catagaggag tttgactatg gggaatgata caacatggac ggataacgcg
     9841 ctcggcagaa aggtaccatc tcgcatagct ggcaaaccgt gtaggtcctt tctgggtgcg
     9901 ttcatcgaca gtacccaagc caaacatgaa aagacgaaaa atggacagtt gcgacgatca
     9961 agcggaatga ctcaacgtca aaaacccaac aagctcgttg cttcgtggga tgaactcttc
    10021 gaacgtctca atattcaaga cggcgccacc atttcttttc atcatcacct aagaaatggc
    10081 gacagaatag tcaacgaagt agttggtcgg ctcgctgcaa agggtgtctg tgacctaaca
    10141 ctggcgccat cggcgctttt tcctgtacac aacgcccttt tgccgtacat aagatctggc
    10201 gtcattacca aaatcgaagg ctctatgaat ggtaccatcg gggccgcttg ctcgcgcggc
    10261 gaaataccca aaacctgcgt tttgcgctct catggcgggc gtcaaagggc tatccaagag
    10321 ggggaccttc ctatcgacgt tgcgtttata gcagcgcctt gtgccgattc gttcggcaat
    10381 gcaaatgggc tttatggtcc ttcggcttgt ggaccgcttg gctatgcaaa acccgactct
    10441 gaatatgctc atcaggtcgt ggttatcacc gacaacttgg taccgtatcc atgcagctca
    10501 tggtcgattt tcggcggcaa tgtcgattat gtcctccaag ttgagagtat cggcgacagt
    10561 tcgcaaatcg tgtcggggac aacactgctg acaaaaagtc ccacacagct cgttatcgct
    10621 cggcttgccg cgcgatttat cgaggccgca ggtattatgc aagagggttt ttcgttccaa
    10681 gcgggagcgg gaagcatatc acttgcgacg atcgtctttc ttgccgaaag aatgcgaaag
    10741 gccggcgtta aagcatcatt tgcacatggt ggggcaacaa aggttcttgt cgatcttctt
    10801 aaagagggat tgttgggttc gatcgtcgat cttcaaagct tcgatcttga tgcagttcga
    10861 tcctgcgcag aaaacgcaag acacattgag tccgacccat acagctcgta tggtccctat
    10921 gcgcgcggct gtgtggccaa tatgttagat gtcgctgttc taggcgcaac tgaaatcgat
    10981 cttgatttca acgtcaatgt cgttacgcac tctgacggca tgctcttgca tggtattggc
    11041 ggtcatagcg acgtatgcag ttccgcggga tgcaccatta tcacggcgcc atcttttcgc
    11101 aagagaattc caatcattaa agagcaagtc acgacaataa cctgtcctgg ggaagcggtc
    11161 gatgtattga taaccgagcg cggaatttcc ataaacccca accgcgaaga cctcatctgg
    11221 cgcgcaaaaa agtttgattt gccatgcgtt gatattgctc agcagatgaa cgacgtatat
    11281 cggatcacgg gcaaaccaga aaaacctcga tttaccgagc tgccggttgc ccttgttgaa
    11341 tggcgcgatg gcagcataat cgacacagtg tggcaaatgg cagaataacc tgatttcccg
    11401 ctttttgtaa ctaggtcgac gaggg
//
LOCUS       DAOOWY010000057        11350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_5707_length_11350_cov_20.155201, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000057 DAOOWY010000000
VERSION     DAOOWY010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11350
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5707_length_11350_cov_20.155201"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PK105_05620"
     CDS             <1..608
                     /locus_tag="PK105_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU61224.1"
                     /translation="LSAIDHYDVTKGYHFISYAVWWIRQAILKAICEKSRMIRLPLNR
                     ANELVQIEKARSLFEGEISEEDEIREVARFLNMEPEHIKDIISISRDLISLDAPVYDE
                     RNSTTVGELIENEKHESPEQNAIQSSLRDDINEVLSTLTAKEREIVEYRFGLNGKRPM
                     SLKEIGDRMHLTKERIRQIEKAALKKVAVPETMEMLQDYVA"
     gene            766..1863
                     /locus_tag="PK105_05625"
     CDS             766..1863
                     /locus_tag="PK105_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU61225.1"
                     /translation="MAEIFLKNEKLFVNLPELGQWLSNEMQDFACSGKMLRGMLAVVG
                     SRLLHDERILVADYEDHKAISAAAGLELLHAGLLVHDDIMDHDHKRRGKPTFHLRIKD
                     ILEKIHANSAAAHAFSIAEAQGICVGDLFFFLGWQEISGLDLSGLDLDISSLFARELA
                     LVTIAQIRDVELGYDKSIPDIDEVIEVYRHKTARYTIVLPLVAGVLVAQSSLARASAS
                     QMARIMDKSIIPLLEDFGESIGIVFQLQDDRIGLFGNEEETGKPVGSDLKEGKKTPYI
                     ILLANRLEKGERERFDSIFASPTQDLKDIEWIRNTVMAKGVEADIQKMIDSYLSKAQA
                     DLNELSALPSIESKTISLLEGLIQYSGSRSS"
     gene            complement(2024..4453)
                     /gene="gyrA"
                     /locus_tag="PK105_05630"
     CDS             complement(2024..4453)
                     /gene="gyrA"
                     /locus_tag="PK105_05630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HOU61226.1"
                     /translation="MAESSNIIPVPIEEEVKSSYLNYAMSVIVSRALPDVRDGLKPVH
                     RRLLYAMDELGLRSNATTKKSARITGDAMGKYHPHGDQSLYDALVRMAQPFSLRYPLV
                     QGQGNFGSIDGDPPAASRYTEAKLSKIGEEMLTDLDKETVDFVPNYDESLKEPSVLPS
                     AIPNLLINGSSGIAVGMATNMPPHNLREIALAIEAFIDDPDITIDVLMKNIKGPDFPT
                     GGIIYGLQGIKDAYETGRGRLTVRGRFIIETMKSGREQIVFTEIPYALNKTMLVTRIA
                     ELVREKQIDGISDLRDESDREGIRIVLELRKGAITKIVLNQLFMRTPLQSTFGVINLA
                     LVDGVPKCLNLKELIYYFVQHRFEVVTRRSKFELKKAEERAHILRGLVIALGNIDEVV
                     AIIKSSKNIDTAKTRLSERFELTEAQAQAIVDMRLGRLTSLETEKILAELKEIEARIE
                     YLKGLLSDPNSIRQVIKQEIHDLAEKYGDDRRTEIVPNQVEQINIEDLIKPEEMVILI
                     SNKGFIKRISVNQYKSQGRGGKGSNSAALIEEDFVEQLFIANTHDYILFISSWGKAYW
                     LKVLEIPETSKQARGPHIRSLLAISEDEEISAIVDFSDFSEKEYILMGTLKGVVKKVA
                     TSEFANAKTRGIIGITLDKGDRLISAVLTQGSDEVVLISRRGQALRIHESQVRKMGRT
                     ARGVRGISLHESDELAAMLRVEPNESMLIITQNGFGKRVSYDLFNVHSRGTKGQTIYS
                     PDEFSGEVVKGVSVQEEDEVMVITSTGKTIKLSVSSVRQMGKGARGVRIVNIEPPDFV
                     IGIDKIVQEQE"
     gene            complement(4482..6398)
                     /gene="gyrB"
                     /locus_tag="PK105_05635"
     CDS             complement(4482..6398)
                     /gene="gyrB"
                     /locus_tag="PK105_05635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOU61227.1"
                     /translation="MESKTSSYSANTIQVLKGLEAVRKRPGMYIGTTGSEGLHHLVYE
                     VVDNSIDEALAGFCKKIVVALERQDIIRVEDDGRGIPVDIHPTEGISALELVMTRLHA
                     GGKFDKKNYKVSGGLHGVGVSVVNALSAWLEVYVHNDGKVFYQRYVRGIAEKPVAFIG
                     DTDRTGTIVRFQADPDIFEETVYSFDVLSNRLRELAFLNKGIKISLIDERLSQPKRIE
                     FHFEGGLQEFVEYLNKNKTVIHKDVIYFLGTRDEVEIEIGMQYNDGYNETMFTYVNGI
                     NTKEGGTHLIGFRNALTKVVNDFFKKSKYSKKIEETLSGDDVREGLTAVLSIKVLEPQ
                     FEGQTKSKLGNTEVKGIVEGFFSEQLDLYFQKNPEVIDIILGKCATAARARIAARQAR
                     ELTRRKSLLEASSLPGKLADCQEKDPSKCELFIVEGDSAGGSAKMGRDRLFQAILPLW
                     GKMLNVEKARLDKVIGNDKLQPIIASLGTGIGEDFDISKLRYHKVIIMADADVDGSHI
                     RTLLLTFFYRYMTELIERGHVYLAMPPLYRINYDKEIHYAYSDEDKDKLLLQSNKDPS
                     KISVQRYKGLGEMNPEQLWETTMDPSRRIIKRITMEDAILAEEMFVTLMGEQVEPRRR
                     FIEENALEVSNLDV"
     gene            6825..8216
                     /gene="dnaA"
                     /locus_tag="PK105_05640"
     CDS             6825..8216
                     /gene="dnaA"
                     /locus_tag="PK105_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOU61228.1"
                     /translation="MSDDNGTIWQKALEIAKSQVPESEFIMWFRLSYIGYENGTILLQ
                     ANNTFLRDQFVRKYSQFMREIISSLLGMPVELSVEAQSKTIDHHIAPQVSPRKAKNNI
                     GAEKVIEAQPGISRSARPSNLQSRYTFESFVVGENNNFAFNAAIAIADKPGSSYNPLL
                     IYGGVGLGKTHLMHAIGNRIVERHADFRIICVTAEEFTNEFIKTIHDRTTNDFKNKYR
                     NVDVLLIDDIHFFQSKHGVQEELFHTFNALYDTNKQLVFTCDRPAAELKDFSERLKSR
                     FMMGLKTDLTTPSFETRAAIVRKKLELYKRHFGEDVINLIAKSIETNVRDLDSCINQI
                     IAYAELIKVEPTFEIAQNVIKQLTNSFMPSAIEVSSIIKTVADYYKLSISDLKGKKRS
                     KNIALARQIAMYIIRETTDYSTTEIGSEFNGRDHTTVMHSCQKIEDLIKFDASFSSLV
                     NHLIKECKEKSSL"
     gene            8543..9643
                     /gene="dnaN"
                     /locus_tag="PK105_05645"
     CDS             8543..9643
                     /gene="dnaN"
                     /locus_tag="PK105_05645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOU61229.1"
                     /translation="MRFVCDRDTFAKEIAIAQDIIASKNAFSIMSNVYLEVIDSRLII
                     KATDAKVGFETEIPVSDSEPGAITVFCDKLLAITNSIPEGDMILEQHDSVIEIKTLSK
                     NVRFQLKTIAGDKYPVIPDIEDNQYFPLRARDLRKMISQTIFSVSNDETRFFMNGVFI
                     EKLPENELVMVATDGRRLAYMSIEEGNIPDFRPAIIPPKVLSVILKRASDEGQIYMAI
                     SEKNIFFRFGNYQLTSYLIEGRFPNYQKVIPQNQRQKFSVSKDELLSALRRVSLFVER
                     SNKVIFDISRRGLVIKSEETELGAAREELSCEYDGDDVVIFLNLRYLEDPLKVMDSEN
                     ITIEFSDPSRAITLRPEPASNYFHVMMPIQQP"
     gene            9644..10762
                     /gene="recF"
                     /locus_tag="PK105_05650"
     CDS             9644..10762
                     /gene="recF"
                     /locus_tag="PK105_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOU61230.1"
                     /translation="MAFEHIRIAQFRNIEEAELELGTGNIFLLGENGQGKTNFLEALY
                     CLSYGNSFRTHQDRDLIMHERSDFLLRAEWSEPGYIVRDQISLRYLPEGRKEILLNQK
                     RLLDRKELVAHNPSVVFCHEDLAFASGAPEERRFFFDQCAGMLWLEYIDLLRSYRRLL
                     KHRNIALKTDTFGVLDTLDEQLARYGSELMFYRKELSRLFEDRFPERYEQVSQLGTEV
                     SIQYRPNWPEEASISEIVETLAHRRQKDIEAGTTLSGPHRDRWVFTMEGRDFSAFAST
                     GQLRLASLILRVVQVYLFSELSQGGKPRFPVLLVDDVLLELDVAKRRRFFSLLPEKEI
                     GAQSVFTFLPEEPWREYANWTTIVYKVSHGRFSREESI"
     gene            10746..11066
                     /locus_tag="PK105_05655"
     CDS             10746..11066
                     /locus_tag="PK105_05655"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOU61231.1"
                     /translation="MKKASEILSRFFDDNMFEAATQFGAFRSTWKKIVGERLADHSRP
                     KGIVHHSLIISADHSGWIQLLQLNQERILARISKSYPELEINGLAFMIEDTKEASPRA
                     EPTT"
     gene            11211..>11350
                     /gene="rpmH"
                     /locus_tag="PK105_05660"
     CDS             11211..>11350
                     /gene="rpmH"
                     /locus_tag="PK105_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011772387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOU61232.1"
                     /translation="MKRTYQPSRVKRNRKFGFRARMKTKDGRKILSRRRAKGRIKLSV
                     SD"
BASE COUNT         3158 a         2567 c         2428 g         3197 t
ORIGIN      
        1 ttctgagcgc tatcgatcat tatgatgtta caaaaggcta tcattttata tcttatgcgg
       61 tgtggtggat acgccaagca attctcaaag cgatctgcga gaaaagccgc atgattcgcc
      121 ttcctctaaa tagagcgaat gaacttgtgc agattgaaaa ggctcgttcg ttgttcgaag
      181 gcgagatttc cgaagaagat gagatacgag aagtagccag attcctaaat atggaacccg
      241 agcatataaa agatatcata tctatttcgc gggacctgat atcgcttgat gctccagtat
      301 atgacgaaag gaattccaca actgtaggcg agttaattga gaatgaaaaa catgaaagcc
      361 ccgaacaaaa tgctattcaa tcaagcctgc gagatgatat taacgaggtt ctttcaaccc
      421 tgaccgctaa agagcgtgag atcgtggaat atcgctttgg gctgaacggt aagcgtccta
      481 tgagccttaa ggagatcgga gatcgcatgc atcttacaaa agagagaatt cgacagattg
      541 aaaaagcagc cttaaagaaa gtggccgttc ccgagaccat ggaaatgctc caagactatg
      601 tggcttaaag ccttgctagc agtttgatgt cttaagccct ggttggtatt cagccagggc
      661 ttttttagtg catctcatcg cgctatactg ctttttatgc aaacatctga tgagatttgc
      721 tttcatgatt tcgctaaaaa atgcaaagat catatctctg agagaatggc ggagattttt
      781 ctaaaaaacg agaaattgtt cgtcaatttg cctgaattgg gccagtggct atccaatgag
      841 atgcaggatt tcgcatgttc aggaaagatg ctaagaggca tgctcgctgt tgtgggatct
      901 cgacttttac atgacgaacg tatacttgta gccgattatg aagaccataa ggctatatcc
      961 gctgccgcgg gcttagagct tttgcatgcg gggctgcttg tccatgatga tattatggat
     1021 cacgaccaca aacgccgagg gaagccaact tttcacctac ggataaaaga tattctcgaa
     1081 aaaatacacg ccaattccgc tgcagctcat gccttttcca tagccgaggc tcaaggtatc
     1141 tgcgtgggag atctgttttt ctttcttggt tggcaagaaa tttctggtct cgatttatct
     1201 ggactcgatc tagatatatc ttctctattc gcaagagaac tcgcgctggt gactatagca
     1261 cagattcgag atgtggaatt gggatatgac aaaagcattc cggatatcga cgaagttata
     1321 gaagtatatc gccataaaac cgcgcgctat actattgttt tgcctctagt cgcgggagtt
     1381 ttggtagccc aatcttcatt ggctcgggct tcggcatctc agatggcgcg aatcatggat
     1441 aaatcgatca ttcctttgct tgaggacttt ggagaatcca taggcattgt atttcaactc
     1501 caagacgaca ggatcgggtt attcggcaat gaggaagaga caggaaagcc cgttggttcc
     1561 gaccttaagg aaggcaagaa gactccatat atcatccttt tggcaaacag gctggaaaaa
     1621 ggagaaagag agcgttttga ttctatcttt gcatctccaa cgcaagattt gaaggacatt
     1681 gaatggatac gcaatactgt catggccaag ggagtagaag cagatattca gaaaatgatc
     1741 gattcatatc tatcgaaggc tcaagctgac ctgaatgagt tgtccgcttt accatcgata
     1801 gaatcaaaga ccatttctct attagaaggc cttattcaat attccggcag ccgatctagt
     1861 taaaaaatca tactgctacg aaccttgttt atgaggattg ggaatggcat aagagagcct
     1921 tctcatgcat aaacatagtg aagtaaaatt cactccgatt catattgttt cacgtgaaac
     1981 aatatagcca ttctgcatag taaagaaggc aataaccgat ttcctattcc tgttcttgaa
     2041 cgatcttgtc tatacctatg acgaaatcgg gcggctcaat atttacaatc cgtacgccac
     2101 gagcaccctt ccccatctgg cgcaccgagg aaacgctgag ctttattgtt ttacctgttg
     2161 aggttatgac catgacctca tcttcttcct gcacagacac gccctttacc acttcaccgg
     2221 agaattcgtc tggcgaatag attgtttgtc ccttagtgcc gcgcgaatga acattgaaaa
     2281 gatcataact tacacgctta ccaaatccat tctgagttat gatcagcata gactcattgg
     2341 gctctactct cagcatagca gcaagctcgt ctgattcatg caaggatatt cccctcacac
     2401 cacgggcggt acgacccatc tttctgacct gagattcatg aattcgcagc gcctgtcctc
     2461 ttctcgatat cagcacaact tcgtcggagc cttgtgtcaa aacggcggat ataagcctgt
     2521 ctcccttgtc gagcgtaata ccgattatgc cgcgtgtttt tgcatttgca aattcgcttg
     2581 ttgcgacctt cttgacaact cctttgagcg tgcccatcaa tatatattcc ttttcggaga
     2641 aatcgctaaa atcgacgatt gcgcttatct cctcgtcttc agaaatcgca agaagcgatc
     2701 gaatatgagg tcccctggct tgcttgctgg tctctggaat ctcaagcact tttaaccagt
     2761 aggccttacc ccagcttgag ataaacagga tataatcgtg ggtgttggca atgaatagct
     2821 gctcgacaaa gtcttcttcg attaaagcag cggaattaga gcccttccct cctcttccct
     2881 gagatttgta ttgattcacc gagatgcgct taataaagcc cttgttagag atgagtataa
     2941 ccatctcttc tggtttgatt agatcttcaa tgtttatctg ctctacttgg ttaggcacaa
     3001 tttcggtgcg tcggtcatcg ccgtattttt ctgccagatc gtggatttct tgtttgatca
     3061 cctgccggat ggaatttggg tctgaaagta atcccttcag atactcgatt cgagcttcga
     3121 tttcctttaa ttccgccaga attttctctg tctcaagact tgtcagtctt cccagtcgca
     3181 tatctacaat ggcctgagcc tgagcttcag tcaattcgaa gcgttcggac aagcgagtct
     3241 ttgccgtatc gatattcttg ctcgatttga ttattgctac tacctcatcg atatttccta
     3301 gagcaattac caggccacga agaatgtgtg cacgctcttc ggcctttttg agttcgaatt
     3361 tggaacggcg agtaactacc tcaaacctat gctgcacaaa atagtaaatt agctctttta
     3421 agttgagaca ttttggtacg ccatccacta gcgccaggtt gatcactccg aatgtgctct
     3481 gtaaaggcgt tcgcatgaat agctgattta gcacaatctt tgttatggct ccttttcgga
     3541 gctccagaac gatgcggatt ccttctctgt cgctctcatc acgaaggtct gagattccat
     3601 cgatctgctt ttcgcgcaca agctccgcaa tgcgggttac gagcattgtc ttgttgagag
     3661 cataaggaat ttctgtaaaa acaatctgct ctcggccact tttcatcgtc tctatgataa
     3721 accgtcctcg caccgtcaac ctgccgcgac ccgtttcgta ggcgtcctta atgccttgca
     3781 agccatagat aataccccct gtaggaaaat cggggccctt aatgtttttc attaaaacat
     3841 caatagttat gtctggatca tcgataaagg cctctattgc gagcgcaatt tcgcgtaagt
     3901 tgtggggagg catattcgtt gccataccta cggctatccc gctcgagccg tttataagca
     3961 gatttggaat agcagatggc aaaacgcttg gctctttcag gctttcatca tagttgggaa
     4021 caaaatccac ggtctccttg tcgagatcag tgagcatctc ttctccgatt ttcgagagct
     4081 tggcctctgt gtagcggctg gccgccggag gatccccgtc tatagaacca aagttcccct
     4141 gaccctgcac aagtgggtat cgcaatgaga aaggttgagc catcctaacg agcgcatcgt
     4201 agagcgactg atctccatgc ggatggtatt tacccattgc atcgcctgta atgcgagcac
     4261 ttttcttggt tgtcgcgttg ctgcgaagtc caagttcatc catagcatac aaaagccgac
     4321 gatgcacagg cttgagtcca tctcgaacat ccggcaacgc gcgcgagacg attacagaca
     4381 tcgcatagtt gagataagaa ctcttgactt cttcttctat gggaacagga atgatattgc
     4441 ttgattccgc cattctaatt tgcctttatt ctaatttgcc tttacacatc tagattcgat
     4501 acctcgagcg cgttttcctc gatgaaacgg cgccttggtt ccacttgttc gcccataagg
     4561 gttacaaaca tctcttctgc aaggattgca tcttccatgg taatccgctt gatgattctt
     4621 ctgctggggt ccatggtggt ctcccaaagc tgttcgggat tcatttcacc caagccctta
     4681 tatcgctgca ccgaaatttt tgaagggtct ttattcgact gcaataaaag cttatccttg
     4741 tcctcatcgc tataagcata atgtatttcc ttgtcgtaat ttatgcggta taaaggaggc
     4801 attgcaaggt agacatggcc cctctctata agctctgtca tgtagcgata gaagaaagtg
     4861 agaagcaagg tccttatatg agagccgtct acatctgcat ctgccattat tatcacttta
     4921 tggtaacgaa gtttcgaaat atcgaaatct tctcctatgc cagtgccaag actcgcaatt
     4981 attggttgta gtttgtcgtt gccaataacc ttgtcaaggc gggccttttc cacattaagc
     5041 atcttgcccc atagaggcag aatggcttga aataacctgt ccctgcccat ctttgcagat
     5101 ccaccggcag aatcgccctc tacaatgaat aattcgcatt tcgatggatc cttttcctga
     5161 caatccgcga gtttgcctgg caggctggat gcttctaaaa ggcttttccg cctggttagt
     5221 tcccgggctt gtctcgctgc tatgcgagcc cttgctgctg tagcgcactt tccaaggata
     5281 atgtctatga cctctggatt cttctgaaag taaagatcga gctgttcaga gaaaaagcct
     5341 tccacaatgc ccttgacttc ggtattgcct agcttacttt tggtttggcc ttcgaattgg
     5401 ggctctaaca cttttatcga tagaacagcg gtaaggcctt ctcgcacatc atcgccagaa
     5461 agagtttcct ctattttctt actatactta gacttcttaa agaaatcgtt gacgactttt
     5521 gttaaagcat tcctaaatcc aataagatgg gtaccgcctt cctttgtgtt tatgccgttg
     5581 acataagtga acatcgtctc gttgtagcca tcgttatact gcatgccaat ttctatctcg
     5641 acttcatcgc gagtacccag gaaataaatc acatctttat gtattacggt cttgttttta
     5701 ttgagatatt ctacgaactc ttgaaggcct ccttcgaagt ggaattcgat tcgtttgggc
     5761 tgagaaagcc tttcatctat aagggatatc tttatgccct tattcaggaa ggcaagttct
     5821 cgtaagcgat tgcttagcac atcaaatgag tagactgtct cttcgaatat atccggatca
     5881 gcctgaaacc gcactattgt gcctgttctg tctgtatcgc caataaatgc aaccggtttt
     5941 tccgctatgc cgcgcacata tctctgatag aatacctttc catcgttgtg cacgtatacc
     6001 tcgagccagg ctgacagtgc attgaccaca gagactccta cgccatgaag cccacctgaa
     6061 accttgtaat tcttcttatc gaattttccg ccagcatgta gacgagtcat aacaagctcg
     6121 agggcggaaa tgccttcggt aggatgaata tctaccggaa tgccccggcc atcatcttct
     6181 acccggataa tatcctgtct ctctagcgcc acgacgatct tcttgcagaa gcctgccagg
     6241 gcttcgtcaa tcgaattatc gacgacctca tataccagat gatggagacc ttcagagccc
     6301 gtagtaccta tatacatacc cggcctcttg cggaccgctt ccaacccttt gagcacttga
     6361 atggtatttg cggaataact gctagtctta gattccatgg ttatcctgaa ttgaattgca
     6421 gaaaataggg gtaagatttg cgataaatct catttcttat cataccaaat agagagaatt
     6481 gagtaaagct caacagcaaa aggtcgtcaa aagctagctc ttctgcattt gtggaaaacc
     6541 tgtgcataac ttgttatctc tttgtggtta gcctgtgtat ttgtgatata atttctttag
     6601 cttacaagag aatccttcaa aataaccggt tcctaaaaaa ttcaataaat aaatagccct
     6661 ataataggcc gcatttcccg tgtttcctaa acaaatagtc aaaaaatatc gaactttgcc
     6721 ctctcggtat cttgcactct ccggtgcttt ggtatacaaa aaatctatgg cttcttgtgg
     6781 aaaacttgtg gaaatgtaca atgatgtctt ggagatttcg acacatgagc gatgataatg
     6841 gtacaatctg gcaaaaagct cttgaaatcg ccaagtcgca ggttccggag tccgaattca
     6901 tcatgtggtt tagactatca tatatcggat acgaaaatgg aaccatcctg cttcaagcaa
     6961 acaatacctt ccttagagat caatttgtcc gaaaatacag ccaattcatg agggagatta
     7021 tttcatccct tctgggaatg cctgtagaat tgtccgtaga ggcgcagtct aaaactatcg
     7081 atcatcacat tgcccctcag gtctcccctc ggaaagcaaa aaacaatata ggtgcagaaa
     7141 aagtaattga ggcacagcct ggaatctctc ggagcgctcg ccccagcaat cttcagtctc
     7201 gatatacctt tgagagtttc gttgttggag agaacaacaa tttcgctttc aatgcagcaa
     7261 tcgcaattgc agataagcca gggtcttctt acaaccctct tctcatttat gggggagttg
     7321 gccttggtaa aacacacctg atgcatgcca ttggaaaccg gatcgtcgaa agacatgccg
     7381 atttccggat catttgcgtt acagccgagg agttcaccaa cgagtttata aagaccatac
     7441 atgatcgcac aaccaacgat ttcaagaata agtatcgcaa tgtcgatgtc ttgctaatcg
     7501 atgatatcca cttcttccaa agcaaacacg gagtccaaga agaactcttt cataccttca
     7561 atgccctcta tgatacgaat aagcagctgg ttttcacctg cgatcgccca gccgcagaat
     7621 taaaggactt ttctgaacgc ctaaagagca gattcatgat gggactaaag accgacctaa
     7681 ctacccccag tttcgaaacg cgagccgcta ttgtaagaaa aaagctcgaa ctctataaac
     7741 gacattttgg cgaagatgtc atcaatctca tagcaaagag catagaaacc aatgtgcgcg
     7801 atctagattc atgtataaat cagatcattg cctacgcaga actcataaag gtagagccaa
     7861 ccttcgaaat cgcacaaaat gtaattaagc agctcacaaa ttcattcatg ccttccgcaa
     7921 ttgaggtatc ttcaataata aagactgtgg ccgactacta taagctctcc atttccgacc
     7981 tcaaaggaaa aaaaagaagc aaaaacatcg cactagccag acagattgca atgtacataa
     8041 tacgagaaac aacagattac tccacaaccg agataggctc cgaattcaat ggtcgagatc
     8101 acacgacagt tatgcattca tgtcaaaaaa tcgaggatct tatcaaattc gacgcttctt
     8161 tctcatcctt ggtaaatcac cttatcaagg aatgcaaaga aaaatcatct ctatgagatt
     8221 cttaatgagg ttcttatcct gttacaaacc tgtggaaaac tcaacaaatc tgatgaagaa
     8281 gaatcaaatc tgtggaaaaa gtgaattact atcaatactt tgccacaagt tttgcacata
     8341 cctaactcta tatttataaa atagatacag tagttatcaa catattcacc gcgcttacta
     8401 ctactattac tatttaatat ataaatagaa ttagtaataa tagtcccaaa cagattgtgg
     8461 agtgtttatc attctaaaac atgctgttta tttttccttt atgagctgct aatataaaac
     8521 ttctgaatgg aggaaaagcc acatgagatt tgtatgcgat cgagatacct ttgcaaaaga
     8581 gatagcaatc gcacaggaca tcatagcttc aaaaaatgca ttttctatta tgtccaatgt
     8641 ctatctcgaa gttattgatt ctagacttat cataaaagcc acggacgcta aagttggctt
     8701 tgagactgag attcctgttt cagacagcga accgggagca attacggtat tctgtgacaa
     8761 actactggca atcaccaatt cgattccaga aggagacatg attctagaac agcatgattc
     8821 ggtaattgag attaagacgc tctccaagaa tgtgcgattt cagctaaaga caattgctgg
     8881 ggataaatat ccggttatcc cagatattga agacaatcag tattttccat tgagagctag
     8941 agacttgcgt aagatgatca gtcagaccat tttttctgtt tctaatgatg aaacgaggtt
     9001 ctttatgaac ggagttttca tagaaaaact tcccgaaaac gaattagtta tggtagccac
     9061 agatggtcgt agactcgcct atatgtctat agaggaaggg aatattcctg attttcgccc
     9121 cgcgataatt ccgccgaagg tgttgtccgt aattctaaag cgtgcttccg atgaaggcca
     9181 aatatatatg gctataagcg agaagaatat tttcttccgt tttgggaatt accagttgac
     9241 aagctatcta atcgaaggca gatttccgaa ttatcaaaaa gttattccgc aaaatcagcg
     9301 ccaaaagttt agcgtgtcaa aagatgaatt gctatctgcc cttaggcgtg tctctctatt
     9361 cgtggagaga tcaaataagg ttatctttga tatttccaga cgaggcctgg ttattaagtc
     9421 cgaagagaca gagctcggcg cagctcgcga agaactgtct tgtgaatacg atggcgatga
     9481 cgtggtaatc ttcctgaact tgaggtattt agaagatccg cttaaggtga tggacagcga
     9541 aaatattact attgagttca gcgatccatc tagagcgatc actctcagac cagagccagc
     9601 ctcgaactat ttccatgtca tgatgcctat acagcagcca taaatggcgt tcgagcacat
     9661 acgaattgcc caattcagga acatagaaga agcagagctc gagttgggca ctggcaatat
     9721 ctttcttctt ggagaaaatg gtcaaggaaa aacgaacttt cttgaagctt tatactgtct
     9781 gagctatggc aattccttcc gaactcatca agacagagac cttataatgc acgaaagatc
     9841 agattttctc ctgcgagctg aatggagcga gccaggatac atagtacgcg atcaaatatc
     9901 gctcaggtat cttccagaag gccgcaagga aatattgctc aaccaaaagc gactcttgga
     9961 caggaaagag cttgtcgctc acaatccttc tgttgtgttc tgtcacgaag atttggcctt
    10021 tgcttccgga gcgcctgagg agcggaggtt ttttttcgat cagtgcgctg gaatgctttg
    10081 gctcgaatac atcgatttgc tccgttctta cagacgattg ctgaagcacc gcaacatagc
    10141 tttgaaaacc gatacctttg gtgtcctgga tactctcgat gaacagctcg ctcgatatgg
    10201 ttctgagctt atgttttacc gcaaagaact ttcacgcctt ttcgaggatc gttttccaga
    10261 gagatatgag caagtctccc aactggggac agaggtttct atccagtaca gaccaaactg
    10321 gcccgaggaa gcatctatat ccgagattgt cgagactcta gcacatcgac gccagaaaga
    10381 tatcgaggct ggcacgacat tgtccggacc acaccgcgat agatgggtat tcaccatgga
    10441 aggccgggat ttttctgcct ttgcctcgac aggtcaactt cgacttgcct ctcttatctt
    10501 gcgcgtagta caagtctacc ttttttcgga actgtctcaa ggcggaaaac cgagatttcc
    10561 ggtattgctg gtagatgatg tacttttgga gctcgatgtg gcaaagcggc gaaggttttt
    10621 ttcgcttctg ccagaaaagg aaattggtgc ccaatcggtt ttcacctttt tgcccgaaga
    10681 accatggcga gaatatgcaa attggactac aatagtttac aaggtaagcc atggaagatt
    10741 ttcgcgtgaa gaaagcatct gagattcttt cccgtttttt cgatgacaat atgttcgagg
    10801 ctgccacaca attcggagct tttcggtcga catggaaaaa aatagttggc gagcggcttg
    10861 ctgatcattc gaggcctaaa ggaatagttc atcactctct catcatttct gccgaccatt
    10921 ctggctggat tcagcttcta cagctgaacc aggagaggat tttggcgcga atatcgaaga
    10981 gctatcctga attggagata aacggcctcg catttatgat agaagacacc aaagaggctt
    11041 cgcccagggc agagcccacg acctaagcag ccgaaaggcg gttgaccaaa ggctcttttg
    11101 cagtctatac tccagcgctt gattgtacaa ttttccggtt gtggcgagag atcctggaag
    11161 agaaatcttg ggaagagctc ttgcaaccgt ttatgcaagg agaatagacc atgaaacgca
    11221 cctatcagcc gagccgagtt aaacgcaata gaaaattcgg ttttcgtgct agaatgaaga
    11281 ctaaagacgg ccgcaagatt cttagcagaa gaagagcaaa aggtaggata aagctctctg
    11341 tctcagatga
//
LOCUS       DAOOWY010000058        11211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_5786_length_11211_cov_12.050376, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000058 DAOOWY010000000
VERSION     DAOOWY010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5786_length_11211_cov_12.050376"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1326)
                     /gene="argJ"
                     /locus_tag="PK105_05665"
     CDS             complement(109..1326)
                     /gene="argJ"
                     /locus_tag="PK105_05665"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOU61233.1"
                     /translation="MKQSRGGVTAAKGFLASGVHVGIRKNKEKKDLALIWSEVPCVAA
                     AVYTTNRVKGQTLIVTQEHLADGRAQAIIVNSGNANTCTGEEGLKAARRMAELVSQYL
                     PVKTEDVVVASTGVIGQQLDVSVIEAGMPDLVKGLSRGGSIDAREAIMTTDTMKKEIA
                     VIEELDGVPVTIGAMAKGSGMIHPNMATMLCFITTDCAIAEPLLRKALKEVVHVSFNR
                     VSVDGDTSTNDMVVVLANGKANNAYIDKEDDNYSDFRDALQFVCVELAKMVARDGEGA
                     TRLIECTVGGAASEEQAESFAKGIITSSLVKTAMFGADANWGRILCAMGYTRSDFDPD
                     KVDISFESPRGYIEVCKAGAPLPFSEEKAKKILNNQEVEIIVDLHLGEAEATAWGCDL
                     SYEYVRINGDYRT"
     gene            complement(1341..2390)
                     /gene="argC"
                     /locus_tag="PK105_05670"
     CDS             complement(1341..2390)
                     /gene="argC"
                     /locus_tag="PK105_05670"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOU61234.1"
                     /translation="MIHVGILGATGYAGLELTRILLQHPEVTALSASSVSFEGKRFEE
                     VYPSMINLQSDVALPMLEKAEDVICKADVIFSCLPHGHAEEAGQQCFEASKPFIDISA
                     DFRFGVYEDIFKKWYGVPYKYPKLHEYAVYGLPELKREIIKSAVLIANPGCYPTSAEL
                     GLIPAIQQGLVDISSIVITSMSGVTGAGREPSQTTHYPEAADAASPYKIGSHRHQPEI
                     DLLLSEIAGEQVQSVFTPILAPMNRGIVSMICFRLLRAVSDGELHSIYTEHYADEPFV
                     RVLPLGATASNRFVKFSNYCDISVHLSADKERAVIISTIDNMVKGAAGQAVQNMNILL
                     GFEETDGLRFIPPAF"
     gene            2831..4906
                     /locus_tag="PK105_05675"
     CDS             2831..4906
                     /locus_tag="PK105_05675"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOU61235.1"
                     /translation="MLSSGVFVAIITALAALGYSLITSSKIKKIPLSNPAVMRISGYI
                     EEGAHAFLAREYKAVGFIIPVVAVLLLCLNSGALKLQAVSFLIGSVLSALSGWFGMSI
                     AVKANARTAEAAQKGILPALRIAFSSGSVMGMTVVGLVLLGIGAILGLLLFMPDAEQI
                     LAQTIFPIVSGFSLGASMMALFARVGGGIYTKAADVGADLVGKVEVGIPEDDHRNPAV
                     IADNVGDNVGDVAGMGADLFESYAGSIVGCMVLGIAISASPELQQRLAILPLIAAGTG
                     VVASLIGTLFVHLSRAKHPQNALNAGSIGASIIAIVLLIPVVRFIIGEGRFSIGSREA
                     GWLGVYLACITGLVAGTVIGLITEYYTGSNSPPVRKIVKSCTTGAATTIISGLGVGMI
                     STVFPILVIGLAIGLSNRLAGLYGIGIAGLGMLLTLGIQLSVDAYGPVADNAGGLAEM
                     AEMEPEVRRITDSLDAVGNTTAAIGKGFAIGSALLTSLILIVAFLSAAELPVEMMNLA
                     NPSVLIGLFIGGMIPFFFSSLAMDAIYRAAFSMIEEVRRQFRAKPGILEGTDEPDYRT
                     CVDISTQTALKSMILPGISAIVAPVLAGFIGGPAVLTGLLVGATVSGGLLAIFMSNSG
                     GAWDNAKKFIESRSEEGKGSSSHRAAVVGDTVGDPFKDTAGPSLNILIKLMAVISLVL
                     TPVLKAVWG"
     gene            5010..5798
                     /locus_tag="PK105_05680"
     CDS             5010..5798
                     /locus_tag="PK105_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61236.1"
                     /translation="MDYEKHLYMIVHPNHSLIASQLEPDRFIKHYVQGSTRYFEGRLI
                     FVEVDPEFRNPYFDIDTAYSELKPHEDGRPKATKFIKSYRVLEHMDYDAFGKLYISNS
                     LGDYVELESKSHEPSTDAEEFRIMLEVNPVRFIVLTKLNYHDFAQFITDPRNTKGAPK
                     TAFTLLEFDSQQFMKEYEENPLIRCYVPGIHPARLAAAIEEVRNTPGKVVKGISLDCP
                     IDHISYKYLKEGFVFAEQNRPCKFYPYLDLGTIERKFYRFWKSM"
     gene            complement(5809..6213)
                     /locus_tag="PK105_05685"
     CDS             complement(5809..6213)
                     /locus_tag="PK105_05685"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOU61237.1"
                     /translation="MELKEGIIAEKEIIVEEKYIASHLGSGGVPVYATPMMVLHMEET
                     SRLAVDHLLEPGKATVGAFIAVKHLAPTPKGMRVRIRSKLAKIEGRMLTFEVEAWDEI
                     EKVGEAEHIRSIIDIARFGERIEKKKALAASK"
     gene            complement(6239..7231)
                     /locus_tag="PK105_05690"
     CDS             complement(6239..7231)
                     /locus_tag="PK105_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU61238.1"
                     /translation="MWLIILVYAGLVCTLTAVSYALMFRPKCKSLEFTRQASLERGEF
                     DENLLNLDWQEWSFASPYGYALKGQYLQGKKNAPAALFVHGITWSRYGMVKYMRPFIE
                     RGWNVAAFDLAGHGASIAPRRFYPSYGFYEKYDIKAGVEALHSLFSDARLYGLFGESL
                     GAASALQYAPLAKEASSRKIDFIVADCSFSSAWEELLEQYREIHIPNFIAWPSAHMTR
                     FFVRLLRGFDLKEASPSKAVMLTDMPIIFAHGMEDSYVPTIMSVQMASARMSNRIGLT
                     DLVLIPGAGHAVGILTDKARWLAAVDSFIDRVFYEEDPMRNSVVCNEYMDEKNK"
     gene            7433..8479
                     /gene="trpS"
                     /locus_tag="PK105_05695"
     CDS             7433..8479
                     /gene="trpS"
                     /locus_tag="PK105_05695"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOU61239.1"
                     /translation="MQKKRILTGDRPTGKLHLGHYVGSLANRVRLQDEYECFFIIADL
                     HTLTTRPEKENIERLPANIYSAVLDYLSVGIDPNKSVIYLQSAVPEVTELSLFFGNLI
                     TVPRLSRVPSLKEMAQNAHIEEMPFGLLGYPVLQAADILLPRAHLVPVGKDNEAHVEV
                     TREIARRFNYLYGETFPVPEVMVSEYGSLVGTDGNTKMSKSLNNAIFLSDDAKTVNKR
                     VMSMYTDPKRIRADIPGTVEGNPVFIYHDAFNQDKAEVEELKARYRAGTIGDVEVKER
                     LARVLNDFLGPIRERRAYYESQKGLVEEIIYEGTLRMRQEARITISMAKKAMGMSSIW
                     NRISRKAEERKKDA"
     gene            complement(8499..9779)
                     /locus_tag="PK105_05700"
     CDS             complement(8499..9779)
                     /locus_tag="PK105_05700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOU61240.1"
                     /translation="MLKVIEDFPVHESDRERANLQLQRIWSGQTETFIDAFRLASKDK
                     STYRWLRFTIKVLLRNPNKRPRIIVGHVVDLDDLINSQEEIRERLVEIDTMRELFTAI
                     NKSLDFGETFSRTTSQLRRIIPFDRACAESLEDGRLVVVAGYGYNESDLSGLVFPAHG
                     VDNPAVRAIEERRIVICNDVRQSFPGFINATEDFATMSWLGIPLIYEKKVIGLLTLDN
                     KELNAYNDQHVRIASELSEYIAIALEHARRHQLVTRQAMTDKLTGLANRYGLETHGIS
                     LFQKAIEEEHSIGVLILDIDHFKQINDTYGHAYGDVVLQTIAMTIRGQIRTRDYAIRY
                     GGEEFVVLLPDLGTREALIVAERLRNKIAQIKMEENKKMPTVSIGIYAAIPGTLDILH
                     EYIRKADMALYAAKEAGRNRSRVWSISPEFYGER"
     gene            9974..11197
                     /locus_tag="PK105_05705"
     CDS             9974..11197
                     /locus_tag="PK105_05705"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HOU61241.1"
                     /translation="MKKPLNEISNSAFYARFNGWFETSSAIGLHLSNKRNISSVSKNL
                     RFLQRGLTGNRGLIASSYMDEWSILQPYLLYYWPISFYEVSAILYELKTRAVLPDINS
                     VLDLGSGPGPASFAAQTFGAMRASLVDKSKRALAMASAIAAKGRRKNLNLEIIEYCAS
                     IEDFCAPKGESYDLIIAANSLNELWADSPDGILRRRDLVLRFLPNLQPEGILIIVEPS
                     AHYTSIPLLELRDSLLAADLNGFSLACLGPCPHSFACPMLDQVKKPCFSEWQWNLPPS
                     VRELADCAGLDRDSLKASWVALKKQKNRGKDKEALQDNKIGSFDSADLRGRIVSEPML
                     NKAGRVRYIVCTNRGLRVTISAPRNDPTAENLGFFGLGRGDLIAANGLEIRGESHFGI
                     APGSQLEVLMRAPRI"
BASE COUNT         2924 a         2694 c         2551 g         3042 t
ORIGIN      
        1 cagagcctct atcaagacat ccgcattgaa atgtttatcc ataaagcacc tcgtaaataa
       61 tcatatctat atctaaggtt tgcgcaaacg gactagcctc attgcatgct atgtgcgata
      121 gtcgccattg atccgcacgt attcataaga aagatcgcaa ccccaggcgg ttgcctcagc
      181 ctctcccaga tgcaggtcta cgatgatttc tacttcctgg ttgttgagta tctttttagc
      241 tttttcttca ctgaaaggta agggcgcgcc tgccttgcag acttcgatat atcctctcgg
      301 gctctcgaaa gagatgtcca ctttgtctgg atcgaaatcg ctgcgcgtat atcccatggc
      361 acagaggatt ctgccccagt tcgcatccgc gccgaacatt gcagtcttta ccaggctcga
      421 tgtaattatg ccttttgcga aagattcggc ctgttcttcg gatgctgctc ctcccacggt
      481 gcactctatc agcctggttg cgccttctcc atcgcgagct accattttag ctagttccac
      541 acagacgaat tggagagcat cgcgaaaatc gctgtagttg tcatcttcct tgtcgatata
      601 cgcattgtta gctttgccat tggcaagaac taccaccata tcgttcgtgg aagtatcacc
      661 atccacgctg acgcgattga agctgacgtg gacaacttct ttaagtgctt ttcttagaag
      721 aggttccgca atcgcgcaat ctgtcgttat aaagcatagc attgttgcca tgtttgggtg
      781 tatcatgccc gaacctttag ccatagctcc tatagtaacc ggcacaccat cgagttcttc
      841 gattaccgcg atttcctttt tcatcgtatc ggtcgtcata atagcctctc tggcatctat
      901 cgagccgccc ctagaaaggc cttttactag atcgggcatg ccagcttcta tgaccgagac
      961 atctagctgt tggccaatta caccggtaga ggctacgact acgtcttcgg tcttgactgg
     1021 aagatactgg gatacaagct ccgccatgcg ccgtgccgct ttgagacctt cttctccggt
     1081 gcatgtgttc gcattgcccg agttgacaat tatcgcctga gcgcggccat ctgcaaggtg
     1141 ttcctgagtt actataaggg tctgaccctt tactctattc gtcgtataca cagccgcagc
     1201 cacacatggg acttctgacc atatcagtgc taaatccttc ttttccttgt tctttcgaat
     1261 tccgacatga acaccggaag ccagaaatcc ttttgcggca gtcacgccac cccgactttg
     1321 tttcatcgca tcctccttac ctagaatgca ggtggaatga acctgagccc atctgtttcc
     1381 tcaaagccca ggagaatatt catattctga acggcctgac cagccgcacc ctttaccata
     1441 ttatcgatag tagagattat tacggctcgc tccttatccg cagataagtg aaccgaaata
     1501 tcgcaataat tcgagaattt gacaaagcga ttagaggcag ttgccccaag gggcagaacg
     1561 cgcacaaagg gttcgtctgc atagtgctct gtatagatcg aatgaagctc tccatccgag
     1621 accgcacgca gaagtcgaaa gcatatcatg ctcacaatac ctcgattcat aggagcaagg
     1681 atcggtgtaa atacgctctg cacttgctct ccagctatct ccgacaacaa aagatcgatt
     1741 tccggttgat gcctatgaga gccgatctta tagggcgaag ccgcatctgc tgcttcggga
     1801 taatgcgttg tttggctcgg ttcgcgacct gctccagtca ctcccgacat cgaagtgatc
     1861 actatgctgg aaatgtcgac aagaccttgc tgaatagcgg gaataaggcc tagctccgca
     1921 gaggtagggt agcatcctgg gtttgcgatt aaaaccgccg atttgattat ctcacgcttt
     1981 agctcaggca aaccatatac tgcatattca tgcagttttg gatatttata cggtacacca
     2041 taccattttt tgaatatatc ttcatagact ccaaatctaa aatcagcaga tatgtctata
     2101 aaaggcttcg atgcttcgaa gcattgttga cccgcttcct ctgcatgccc atgcggcaaa
     2161 caggaaaata tgacatcggc tttgcatata acatcttcgg ccttttctag cattggaagc
     2221 gctacatcgc tctgaaggtt tatcatagat ggatagacct cttcgaatct tttgccttca
     2281 aagctcacag atgaagcgct caatgcggtt acttctgggt gctgcaagag gattcgggtt
     2341 aactcgagcc cagcatagcc agtggctccc aatataccca catgaatcat gggcatctcc
     2401 tttcaataac aagtgtaatg agaaatggga ttaattgcac gtaaaagtgc aaggttgtca
     2461 gcgacgatag cagaatagat aaagacgacg ggaatggaac gcaagaaatg ttcttcgcag
     2521 agttctcaaa gatggtgagg aaataggtat gagaagtgag cattccatgt gcccctccca
     2581 ataagaaaag tatttcaata aaaatacaaa ttataaattt tgtcaaggat acagatggat
     2641 agcagtttca ttaattttgc ataaaactgt ggtttggccg atacacaatt accatgtttt
     2701 gattttcata ggcattgtca tatctttgtt tgaggtcttg tatttatttt caatattgcc
     2761 agatccgaaa aatcagcctt ataatgaaac gtaaagtaca atttggcttt tcgcaatgag
     2821 gaggtcgccg atgctctcta gtggagtatt cgttgcaatc ataacagccc tggcggcgct
     2881 ggggtattcc ttgattacga gctcgaagat caaaaagatc cccctttcca atcctgccgt
     2941 aatgcgaatt tctggctata tcgaagaagg agctcatgct ttcctggcta gggaatacaa
     3001 ggcagtgggc tttatcattc cggtggttgc tgttctcctt ctttgtctca atagtggcgc
     3061 actgaaattg caggctgttt cctttcttat tggttcagtt ctgtccgcgt tgtccggctg
     3121 gttcggtatg agtattgcgg tcaaggccaa tgccaggact gcggaggctg cccagaaagg
     3181 tattttacct gcgctcagaa ttgctttttc aagcgggtct gttatgggca tgacagtagt
     3241 ggggcttgtg ctcttgggaa taggtgctat cctgggcctt cttcttttca tgccagatgc
     3301 tgagcaaata cttgctcaaa cgattttccc aatagtatcg ggtttttcgc ttggagcctc
     3361 gatgatggcg ctttttgcac gtgttggcgg cggcatttac actaaagcag cggatgttgg
     3421 tgccgacctg gtaggaaagg tcgaagtcgg tatacccgaa gatgatcaca gaaatcctgc
     3481 tgtcattgca gataatgtcg gtgataatgt gggcgatgtt gctggtatgg gagcagacct
     3541 tttcgaatcc tatgccggat ctatagttgg ctgcatggtg cttggaattg caatttccgc
     3601 cagcccggaa cttcaacaga gattagctat attgccactc atcgccgcgg gaacaggggt
     3661 ggtggcttca cttatcggaa cgcttttcgt tcacctaagc cgagccaaac acccccagaa
     3721 tgcgcttaat gcgggctcta taggagcatc tataatagcc atagtccttt taataccagt
     3781 cgttcgcttc attatcggag agggccgttt ttcgattgga tctagagaag cggggtggct
     3841 aggagtctat cttgcatgca taacaggctt ggtggcgggt acggtaattg gtctaataac
     3901 cgaatactac acgggctcga attctccgcc ggttagaaag atagtaaaat cctgtacaac
     3961 cggagccgca accacaatca tctctggcct gggtgtcggt atgatttcaa cggtattccc
     4021 tatcctggta attggactgg ctatcgggct ctcgaaccgg cttgcagggc tctatggcat
     4081 agggatcgct ggcctcggaa tgctgctcac tctcggaata caactctctg tcgatgctta
     4141 cggtcctgtt gcggataatg cgggaggtct tgcggagatg gcagaaatgg agccggaagt
     4201 acgaagaata accgattcgc tagacgccgt tggtaataca acggcagcca ttggcaaggg
     4261 ctttgccata ggatccgcgc ttctcacttc tctcatccta attgttgcct tcttgagtgc
     4321 cgcagaattg cccgtagaaa tgatgaacct tgccaacccc agtgtgctca ttggtctttt
     4381 cattggcgga atgattccct tctttttcag ctcgctagcc atggatgcca tctatcgagc
     4441 cgcattctcg atgatcgaag aggtgagaag gcagttccgc gcaaaaccgg gtattctcga
     4501 aggaacagat gaacctgatt accgaacctg tgtcgacatc tctacacaga cagcactaaa
     4561 gagcatgata ttgccaggta tttcggccat agtcgcgccc gtcttggcag gcttcatcgg
     4621 tgggcctgcc gtgctcaccg gattattggt cggcgctacg gtctccggag gtcttcttgc
     4681 catatttatg tcgaattctg ggggagcctg ggacaatgcc aagaaattca tagaatcgcg
     4741 tagcgaggaa ggcaagggct ctagctctca ccgcgcagcc gtagtaggag atacagtagg
     4801 cgatcccttc aaggatacgg ccggtccctc tctaaatatt ctgataaaat tgatggcagt
     4861 gatttcgctt gtacttactc ctgttctaaa agcagtatgg ggttgatgag tctgcgatcg
     4921 atgttttgac ctattgcaga aatttcttta aatatgtaag aattgcgata atatatttag
     4981 gctgtttgga accttaggag gttcacccta tggattatga aaaacatcta tatatgattg
     5041 tccatccgaa tcactcgctc atagcttcgc agctcgagcc agaccgtttt ataaaacact
     5101 atgttcaggg ttccacgcga tacttcgaag gcaggcttat ctttgtagaa gtcgatcctg
     5161 aatttagaaa cccctatttc gacatagata ccgcttattc ggaactaaaa cctcatgaag
     5221 acggtaggcc aaaagccaca aaattcataa agagctatcg ggttttagag catatggatt
     5281 acgacgcatt tggaaagctt tatatcagca attcgctggg agattatgtg gaactagaat
     5341 ccaaatctca cgaaccttcg acagatgcag aagaattccg tatcatgcta gaagtcaatc
     5401 cagtgcgctt cattgtgcta acaaaactca actatcatga ctttgcccaa ttcataaccg
     5461 acccgcgcaa cactaaaggt gcaccaaaga ctgctttcac actcctcgag ttcgatagtc
     5521 aacaattcat gaaagaatat gaggaaaatc ctcttatccg ctgctatgta cctggcatac
     5581 accccgcaag gctcgccgca gccatagagg aagtccgaaa cacgccaggc aaggttgtca
     5641 agggcatttc cctggattgc ccgatcgacc atatctcata taaatatctc aaggaaggat
     5701 tcgtcttcgc tgagcagaat agaccatgca aattctatcc ctatctcgac cttggcacta
     5761 ttgaacgaaa attctataga ttctggaaga gtatgtgatt tagagtacct atttcgaagc
     5821 ggcgagagct ttctttttct cgattcgctc tccaaatctt gcaatatcta taatggaacg
     5881 aatatgttcc gcctcgccga ctttttctat ttcatcccag gcctcgacct cgaaggttag
     5941 catgcgccct tcgattttcg caagcttaga tcgaatgcgt actctcattc cctttggagt
     6001 cggagcaagg tgtttgacgg caataaaggc gcccaccgtg gctttgcctg gttcaagtag
     6061 atgatccacc gcaagtcgag aagtctcttc catgtgcaac accatcatcg gtgtcgcata
     6121 gacagggacg cctcctgatc ccagatgcga agcaatatat ttttcttcca cgattatttc
     6181 cttttcggca ataatccctt ccttcaattc catttctgct ctcctcgcta ttagctctct
     6241 atttgttctt ttcatccata tattcattgc atactacgct attgcgcatt ggatcttctt
     6301 cataaaatac tcgatctata aaagaatcga cagccgccaa ccatcgagcc ttatcggtca
     6361 aaattccaac cgcgtgtcct gcgccaggaa ttagaactag gtccgtcaat ccaatgcgat
     6421 tcgacatccg agcgcttgcc atttgcacac tcattatggt tggtacatag ctatcctcca
     6481 ttccgtgagc aaagataatt ggcatatcgg tgagcattac tgcctttgaa gggcttgcct
     6541 cttttagatc gaagcctcgc agaagacgga caaaaaatct ggtcatatgg gcactaggcc
     6601 atgcaatgaa gttgggaatg tggatttccc tatactgttc gaggagctcc tcccaggccg
     6661 aagaaaatga acagtctgct acaataaaat cgatttttct ggaggatgct tcttttgcaa
     6721 gaggtgcata ttgaagggct gaagccgccc caagcgattc accaaaaaga ccatagagcc
     6781 gagcatcgga aaatagcgaa tgcaacgctt caactccagc tttaatatca tatttttcat
     6841 aaaaaccata ggatgggtag aaacgccttg gcgcaatact ggctccatgg ccggcaagat
     6901 cgaaagccgc aacattccag ccgcgctcta taaatggtcg catgtatttt accattccat
     6961 agcgcgacca agttatgcca tggacaaaga gcgctgccgg tgcattcttt ttgccctgaa
     7021 gatactggcc tttcaaggca tatccatagg gcgaggcaaa ggaccattcc tgccaatcga
     7081 gatttagaag attctcgtcg aactcgccgc gctcaagaga ggcttgcctt gtaaattcaa
     7141 gactcttgca ttttggccta aacataagtg cataagacac cgctgtaagt gtgcatacca
     7201 gaccggcata aaccaagatt atcagccaca tacaagcatt ctagcgcatt ttggattggc
     7261 tgggcagggc cgcaaattac cacgcgcgtc tagatccaag cctatatccg cgtcaagacc
     7321 tgctgccata ctattatgaa ccacaatttc tttgctcatt gccctttttt ttctgtttcg
     7381 ctacactgtt tcaatacttc atacttaaga gaaataggtt cggagaatta tcatgcaaaa
     7441 aaagcgcatt cttacaggcg atagacccac tggaaaattg catctcggtc attatgtcgg
     7501 ttcactggca aatcgtgtgc gcctgcagga cgaatatgaa tgcttcttca taatcgcgga
     7561 tctacacaca ctcactacca gaccggaaaa ggaaaatata gaacgccttc ccgcaaatat
     7621 atactctgcg gtcctggact atttatcggt tggcatagat ccaaataaat cggtaatcta
     7681 tttgcagtca gcggttccag aagtgaccga gctctcgctc ttcttcggta atctaataac
     7741 tgtacccaga ctctctcgcg taccaagtct caaggaaatg gcacagaatg cgcatatcga
     7801 ggagatgcct tttggccttt tgggatatcc ggtgctgcag gcagccgata ttctactccc
     7861 tcgcgcacat ctcgttcccg tgggcaaaga caacgaagct catgtcgaag ttacgagaga
     7921 aattgcaaga cgttttaact atctatacgg tgagaccttt ccagtgccgg aagtgatggt
     7981 ctcggaatac ggctcgctcg ttggtaccga tggaaatacc aagatgtcca aaagcttgaa
     8041 caatgccatc tttctttccg atgatgcaaa aaccgtgaac aaacgtgtca tgtcgatgta
     8101 taccgatcca aaacgtataa gagcggatat tccaggcacc gtcgagggaa atcccgtctt
     8161 catatatcac gatgctttta atcaagataa agccgaagtc gaagagctca aagcgcgcta
     8221 tagggctgga accatcggcg atgtcgaagt aaaagaaagg ctagcccgag tcctgaatga
     8281 ttttctgggt cccatacgcg aacgtcgagc ttactatgaa tcacaaaaag gccttgtcga
     8341 ggaaatcata tacgaaggca ctcttcgtat gcgacaagag gcaagaatca ctatttccat
     8401 ggcaaaaaaa gcgatgggca tgagctcaat ctggaatcgt atttccagaa aggcagaaga
     8461 gcggaaaaag gacgcataaa gagcctagag tatgctgctc atctttctcc atagaactcg
     8521 gggctgatcg accagactcg agagcgattt ctgcccgcct cttttgccgc atatagagcc
     8581 atatcagcct tccttatgta ctcatgcaga atgtcaagtg tgccgggaat ggctgcatag
     8641 attcctatgc ttactgttgg cattttttta ttttcttcca ttttgatttg agcaattttg
     8701 ttgcgaagcc tctccgcaac aatcaaggct tcgcgcgtgc ctagatcagg aaggagcaca
     8761 acaaattcct cccctccata gcgaattgca tagtcccgtg tacgaatctg cccacgaata
     8821 gtcatagcta tcgtctgaag aaccacatca ccataagcgt gtccgtatgt gtcgtttatc
     8881 tgtttgaaat gatcgatgtc caggataagt acgccgattg agtgctcctc ttctatggcc
     8941 ttttgaaaaa ggctgattcc atgagtctct aaaccataac ggttagccag ccctgtcagc
     9001 ttgtcggtca tggcctgtct tgtcacgagc tgatgcctgc gcgcgtgctc tagagcaatg
     9061 gcaatgtatt ctgatagctc gcttgcaatt ctgacatgct gatcgttgta ggcgttcaat
     9121 tccttgttat ctagagtcag caatccaatc accttctttt catatataag aggaataccg
     9181 agccaagaca tagtggcgaa atcttctgta gcattgataa aacctgggaa tgactgccta
     9241 acatcgttgc agatgacaat cctccgctct tcaatggcgc gcacagccgg attatcaacc
     9301 ccatgagcag gaaaaacaag gccactgaga tcgctttcat tgtagccata acccgcaacg
     9361 acaaccaatc ttccatcttc gagggattcc gcgcaggctc tatcaaaggg aataatgcgg
     9421 cgcagttggc tagtggttct cgaaaatgtc tcaccaaaat cgaggctctt attgatggcg
     9481 gtaaaaagct ctcgcatggt atcgatctcg acaagacgct ctcgtatttc ttcctgagaa
     9541 ttgatgagat cgtcgagatc tacgacatga cccactatta ttcttggcct tttattggga
     9601 ttccgcaaca gaacttttat cgtaaagcga agccatctat aggtcgattt atccttcgaa
     9661 gcgagtcgaa aagcatcgat gaaagtctct gtttgacctg accagatacg ctgtagttgt
     9721 agattcgccc tctctctatc ggactcatgg acaggaaagt cctctatgac cttcagcaag
     9781 tccgagtctt catatccaaa agatcgaaga gttgtacagg attgcgcggt gtgagataga
     9841 aagtcaatat aaaagatatc ggtttcctta aagaaatccg aaagtgaaga atctaggaaa
     9901 aaatccctta agatagaagc ttcgagtttc actatactat aaaattatat gttatgatga
     9961 tgattctgta tatatgaaga aaccactcaa tgagatatca aattcggctt tctatgcacg
    10021 gtttaatggc tggtttgaaa caagttcagc gataggccta catttatcca ataaaaggaa
    10081 tatctccagc gtctccaaaa accttcgatt tcttcagcgt ggccttacag gaaatcgagg
    10141 actcatagcc tcttcatata tggacgagtg gagcattttg cagccttatt tgctatacta
    10201 ttggcctatc tccttttacg aagtatcggc aatactctac gagctaaaaa cgagagcggt
    10261 gcttccagat attaattccg tattggattt agggagcgga cccgggccgg cttctttcgc
    10321 cgctcagacg tttggagcca tgcgggctag tcttgtggat aaaagcaagc gtgcactggc
    10381 gatggcctcc gccatagctg ccaaaggaag gcgtaaaaat cttaaccttg agatcataga
    10441 atattgtgct agcatcgagg acttctgcgc tccaaagggc gaatcctatg atctgatcat
    10501 cgcggctaac agcctcaatg agttatgggc cgattcgcca gatggaattt taaggcgacg
    10561 ggatcttgta ttgcggttct tgcccaattt gcagcctgag ggaatcctca taatagtgga
    10621 gccttccgct cactatacga gcattccgct ccttgagttg cgcgatagtc tgcttgcagc
    10681 agatttgaac ggcttttctc ttgcctgctt aggcccctgc ccgcattcct tcgcttgccc
    10741 aatgctggat caagttaaga aaccttgttt ctcagaatgg caatggaatc ttcccccttc
    10801 ggttcgcgag cttgcggatt gtgctggact tgatcgcgat tctctcaaag cctcctgggt
    10861 agccttgaag aaacaaaaaa atcgagggaa agacaaagag gctctgcaag acaataagat
    10921 aggctctttt gattccgctg atcttcgcgg tcgcattgta tctgaaccca tgctcaacaa
    10981 ggctggaaga gttcgctata tcgtgtgcac aaatagaggt ttgagggtta cgatttcagc
    11041 gcctcgaaac gacccgacgg ctgaaaacct agggtttttc gggcttggac gcggcgacct
    11101 cattgctgca aatggtttag aaattcgggg ggaatcgcac tttggtattg cacccggctc
    11161 acagctcgaa gtattgatga gagccccaag gatttagaaa gcaaacaaaa t
//
LOCUS       DAOOWY010000059        11115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_5851_length_11115_cov_7.616094, whole genome shotgun sequence.
ACCESSION   DAOOWY010000059 DAOOWY010000000
VERSION     DAOOWY010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5851_length_11115_cov_7.616094"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK105_05710"
     CDS             complement(<1..225)
                     /locus_tag="PK105_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61242.1"
                     /translation="MRMKHPWNANWWFRQGPVEEEFLNEDWPGPRNANSAENRAITNT
                     ELSKTSDVQINVEGWKAIHLPHNMTEAPFNS"
     gene            complement(222..1358)
                     /locus_tag="PK105_05715"
     CDS             complement(222..1358)
                     /locus_tag="PK105_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61243.1"
                     /translation="MMNERGEIPVEIVHLYAYDVGRSIDLDKVASLVPAHRDIALAKR
                     RDTPAYLTLPKPLVLSISTEECNSKLFGKISAMAKIYEDGAITMIVRYCTMSTFEDLP
                     AKAHMPIRDVSGSSSIEQFAEASFRMVREALRSAIVGYKELSEGDRETYIAFCLLECP
                     GDDPQAFVNAHKDMAASLISGETVGTLHISQIEQILAKPFSYRKNDIAIFDLDRCLII
                     DPSADYDDVLMMAEHANYRLIELRELDFLLDEWLGEAERDIRRLYFSSRRKRSSERAL
                     RLKLAQIQGLRFDALFTLENLDNSSKIIGDYFLGSIYHRLCEIFNTDEWKFSVERRLN
                     ALQNIYELLKSDTTEQRMMTLEMVFIAVCIIFPILQILQVMLVK"
     gene            complement(1355..2461)
                     /locus_tag="PK105_05720"
     CDS             complement(1355..2461)
                     /locus_tag="PK105_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOU61244.1"
                     /translation="MAKKDKKDIKDFTKILKETASKAPQKVSKVAKTVADSASEQHEH
                     ISKWTKEQKRTFVWFAIILFLVMVIVAVLVFFLALRGEERTMVPDVRRMDLADALVKL
                     QQRELYPRLTLRFTDNPLDRNLVLEQSPPPGSIVKAGRRINLVVSRGAVLDRVEDYSG
                     RNIDDLRLYLQGISTTTKTLVSIREPPLYVFDNSAPGTIIDQEPKPGKEISGPTVLDL
                     VVSKGPESRNIAMLSLLGLSMREMASKASTIPLVFSYKMRSAHEKETPGTVVEQSVQP
                     DTNLKQFDSVQITIATPEARKGYVSGVFEYSLPGYPYVVPVGLDVISPDGTRNSIYSV
                     KHPGGVFSAPFSVQTGSVLVLMVSGAEVTRKEVK"
     gene            complement(2551..3513)
                     /gene="fmt"
                     /locus_tag="PK105_05725"
     CDS             complement(2551..3513)
                     /gene="fmt"
                     /locus_tag="PK105_05725"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOU61245.1"
                     /translation="MRVLFAGNPDIAVPSLESLYDSSHEVVGILTNPETKAGRGLRTN
                     STPVAQAALRLWGDSVPVLTFETLKSEARAAVLAILPDILVSFAYGRIFGPRFMELFP
                     KGGINVHPSLLPRWRGSSPIPHAIMFMDEETGISIQTIAPEMDTGELLNTHRIPLTGR
                     ETSASLSHLCAQLAGPMVVEVLDEIENGIAESRPQLGEPTYCRKITKKDGHIDWHRTS
                     REIDARIRAFDPWPGSFAFLRDMRLCILEAEPFDDFDPMSKAGYVTNPSPGTIIAVVM
                     QKGIIIRTGDGYIAVKRLQMATRKAVGFQEFACGARDIIGAVLH"
     gene            complement(3532..4020)
                     /gene="def"
                     /locus_tag="PK105_05730"
     CDS             complement(3532..4020)
                     /gene="def"
                     /locus_tag="PK105_05730"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOU61246.1"
                     /translation="MFNILTYGESILSEKAHEVTEFDHSLEQLIQGMHEVMRRNNGIG
                     LAASQVGIPRRLFIVGIGDEPLRVFINPHIIARSQETTEFEEGCLSFPGLYFNVVRPL
                     AVDIEAVDMHGKPLRISADGLLARVIQHEYDHLDGILFIDRVSPVKRKRALAHYGRML
                     NM"
     gene            complement(4107..4592)
                     /locus_tag="PK105_05735"
     CDS             complement(4107..4592)
                     /locus_tag="PK105_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOU61247.1"
                     /translation="MATEMPTRYLSKGLSIMPFMVILILFAACNSSQGTDRPNPKLKT
                     VDLTIGNAKIKAEVALTEGERNRGLMYRSSLKDGEGMLFVFESDQKLAFWMKNTKLPL
                     SLAYILSDGTIVQIIDLVPFSEESRPSIRSVRYALEVPQGWFERSGIKVGDKVRIPSL
                     E"
     gene            complement(4766..4972)
                     /gene="rpsU"
                     /locus_tag="PK105_05740"
     CDS             complement(4766..4972)
                     /gene="rpsU"
                     /locus_tag="PK105_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOU61248.1"
                     /translation="MSQITVEDNEPLEKALKRFKRMVEKEGIIREWKRREYYEKPSAI
                     RNREKKALERKLMKKTRRSQEGRY"
     gene            5106..6506
                     /locus_tag="PK105_05745"
     CDS             5106..6506
                     /locus_tag="PK105_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61249.1"
                     /translation="MLFSLGNLITLAIVIAMFVAYHLWTADNRSLEKLKRLGDKLKDE
                     LGSYVESKAEEIKHYGIDLDVQQKAAKVVLEKIQEAQATLDEKSDSITEIADCFKKYD
                     NVLSQLMDMTKRVDQNIAHLAEKNDFIQAITRRLEDADKEIDIIEKEMPKLKEQFAHD
                     AQQILDGFRDDILDQLHERLTGIINLFEKSRSDAESAVTEAASLKENIHEMVEAALSA
                     ATSRAQSIEDSAFATLRAHIANNLEELSHQTDARIEGLSQRIGELVNAINAKLTTLAS
                     ETASQLAESDRRLSEARAALSAMTSDMARIQKESAEITVALSKEFKAERSSLEEQFEQ
                     FGQAFEIHRASFEREFLDEISALRKSLEDAKANSEELRSAAQGNIAEALDELERNVAR
                     ELESRRSALDERIDEWLNSMDAKIRKVSQDALEQRKAEEARLAQEVNAELKRLKDSLY
                     TQTQRIERDIEAFKSV"
     gene            complement(6510..7214)
                     /gene="deoD"
                     /locus_tag="PK105_05750"
     CDS             complement(6510..7214)
                     /gene="deoD"
                     /locus_tag="PK105_05750"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU61250.1"
                     /translation="MSIHIAAQKGQIADRILLPGDPLRAKFVAENYLEGAFCFNEVRN
                     MFGYTGTYRGKPISVMGTGMGMPSLSIYVNELIREYDVNKLIRIGTCGSMLEYLNVRD
                     IVLAMSACTDSAANHLRFKGMDYAPTASFRLLKAAWDAATSRGLRVFAGSILSSDMFY
                     TEDPEQWKLWAKFGVLAVEMETAELYTLAAKFGCEALSVLTVSDDLVKGEITSAEERQ
                     SSFNEMVELALDAIFA"
     gene            complement(7434..8348)
                     /locus_tag="PK105_05755"
     CDS             complement(7434..8348)
                     /locus_tag="PK105_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61251.1"
                     /translation="MRRNFRVSSALFLILMMAMVLMVVSCKKAKVVEKPLEGGTDKTA
                     AQDIAAMPGPRPEAGANLPEIKKQNENILIVYKHTNPGYAEAYGFDTLILRALTDGSG
                     MIVGGTLYQRTASGEREFAQFNASISGDTISLAASSSEQKPRSYTIKTKADRVEVSGA
                     RDVVATLNKSLVFSSPDGSYKEIYSIDSSKENLLSEIQKANVTLEKGSWTYPENGKAV
                     YNQTKPEDTQNIEGFQITLWYQDKGDYRFATEGPEPLNEVYASGFAEALAGDYGLVNA
                     VMLDLMLSESTYLRPIYAFAISRKGLGK"
     gene            complement(8545..9288)
                     /locus_tag="PK105_05760"
     CDS             complement(8545..9288)
                     /locus_tag="PK105_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61252.1"
                     /translation="MRTKKAILIAACLLLGAMLQPFAYAVQGEAIIPGMRLLNLGEPL
                     GQDECAAVTGGDVRMSLNRDRTKLKVNVIDNEYEARLGRIPPKEVFEIDVHNRVVDTQ
                     KAPFMPADATGRKFASSGAVTSKPSQFPEGYWSVTSVKPREDKYGPYMISTNAVGKVE
                     TYVNDGKGNYNASGVYKDTGYAVHSNTNSFDVSKSHGCLIIKQDDLSRVAGVLKKDRE
                     ENPKAVQKILVSPPATKKPRRLFGNRDDL"
     gene            complement(9360..10103)
                     /locus_tag="PK105_05765"
     CDS             complement(9360..10103)
                     /locus_tag="PK105_05765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU61253.1"
                     /translation="MYTRFEPGYILDAHLIEELQGVKADGAFFATLDHDLDVFILRHG
                     QSKGNASNTYQGRYDFPLTAQGEKEARQAGEWLSQFKPDYILASPMLRAKRSAEIVSS
                     VIDLAHIEFDDILIEMDTGVFSGIDPSTAAQRYPDIWEDFYRRSWDAIPAAESSKSIY
                     ARSIIAWQHIRDIATRGASRIICITHGGLLQWLIRSTLGVHDWLPLLPITNCCISKYE
                     IEIIKTGVIAFAQWTLVNLHPLESSCKIR"
     gene            complement(10152..>11115)
                     /locus_tag="PK105_05770"
     CDS             complement(10152..>11115)
                     /locus_tag="PK105_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU61254.1"
                     /translation="LAYNLYTSEISQTPGMASTVSIILMLCASVVIVLQQWVSSRRKY
                     ASMLVNRPVIKQIKGIKSVLAHILCYGIVFFSTLPLAVIVYFSFRKTSGPVFKPGFSL
                     DSYRQIFFDVPKTVTNSFLYSITAVLLIAFVGTLLGFVIARKRNAAVRILDPLLMIPY
                     MVPGTVLGIGFIVAFNKPPIILTGTAAIIILTYFIRRLPYSVRSAASILKQIDPALEE
                     AGINLGSPPGRTFRTITLPLMQSGIISGAIMSWVTSMNELSASILLYVGKTMTMPIKI
                     YISVADGYFGTASAMSTILLFVTGIAMFIVNKYFNRGSETFIAA"
BASE COUNT         2928 a         2693 c         2442 g         3052 t
ORIGIN      
        1 agagttaaaa ggggcttcgg tcatgttatg gggcagatgt atcgctttcc agccttcaac
       61 atttatctgc acatcgcttg tcttggataa ttctgtgttt gtgatagctc tgttttcggc
      121 agaattggca ttccgtggac ctggccaatc ttcatttaag aattcctctt caaccggccc
      181 ctgcctgaac caccaattgg cattccatgg atgcttcatt ctcatttaac cagcattacc
      241 tgaaggattt gcagtattgg aaagataatg cataccgcaa tgaaaaccat ttccagggtc
      301 atcatgcgct gctctgtggt atcgctcttg agtagttcat agatattttg taaagcattg
      361 agacgccttt ctaccgaaaa tttccattcg tcggtattga aaatctcgca tagcctgtga
      421 tagatcgagc ccaaaaaata atctcctatt atcttggagg aattatcgag attttcgagc
      481 gtgaaaagtg catcgaaacg caagccttgt atctgagcaa gcttgagtct cagggctctt
      541 tcgctagacc tttttcgcct gctggaaaaa tacagtcgtc ggatgtcacg ttcggcttct
      601 cccaaccatt catcgagcag gaaatcgagt tctcgcagtt caatgagtct gtagttcgca
      661 tgctctgcca tcatgagcac atcgtcgtaa tcagcagagg gatctatgat gagacagcga
      721 tcaagatcga aaatcgcaat gtcgtttttg cgatagctaa agggcttggc tagtatctgt
      781 tctatctggc ttatgtgcag tgttcctacc gtctctccag atatcaagct tgctgccata
      841 tccttatgag cattgacaaa agcttgcgga tcgtctccag gacattccag cagacaaaag
      901 gctatataag tttcccggtc gccttcgctt agttccttat atccgactat agcgctacgc
      961 aaggcctctc ttaccattct aaaggaagcc tcggcaaact gctctatgga agaggagccg
     1021 ctcacatccc gaattggcat atgtgctttg gccggtaaat cttcgaaagt agacatggtg
     1081 caatagcgca caatcatcgt aatagcccca tcctcataga tctttgccat tgcggatatt
     1141 ttgccgaata gcttcgaatt gcattcctcg gtgctgatgg aaagcacaag aggtttgggc
     1201 agagtcaaat aagctggtgt gtcgcgccgt ttggctaagg caatgtctcg atgcgcggga
     1261 actaaagaag cgactttatc gaggtctatc gatcgcccta catcgtaagc ataaagatga
     1321 acaatctcga ctggtatctc gccacgctcg ttcatcattt cacctccttg cgtgtcacct
     1381 ccgctccact gaccatcaat accagcacag agccagtctg tacagagaat ggcgcgctga
     1441 atacgccacc cggatgcttc accgaataaa ttgaattcct tgttccgtcc ggagaaatga
     1501 catcgagccc aacaggtacg acatagggat acccgggcag ggaatattcg aatacgcccg
     1561 atacatagcc cttccgcgct tcaggtgtag cgattgtgat ctgcacagaa tcgaattgtt
     1621 tgagatttgt atcgggctgg accgactgtt ccacgacggt acctggcgtt tctttctcat
     1681 gcgctgaacg cattttgtag ctgaatacta gcggaattgt ggatgcctta gacgccatct
     1741 ccctcatgga gaggccaagg agcgaaagca ttgctatatt cctggattct ggccccttgc
     1801 ttaccacaag gtctaagaca gtcggacccg atatttcttt gcccggcttt ggttcctgat
     1861 ctatgattgt gcccggcgct gaattgtcga aaacatagag aggcggctct cggatgctca
     1921 cgagggtctt tgtcgtggtg gaaattcctt gtaggtagag cctgaggtca tcaatgttgc
     1981 gacctgaata atcttcgact ctgtcgagca ctgcgccgcg gctcacgacg agattgatgc
     2041 gtcgtcctgc ctttacaata cttccaggcg gaggtgactg ctccagcaca agattgcggt
     2101 ccaggggatt atcggtgaac cgtagagtca atcgagggta tagttcgcgc tgttgaagtt
     2161 tgacaagagc atccgcaaga tccatacgtc ttacatccgg aaccatcgtt ctctcttctc
     2221 ctcgaagcgc gagaaaaaat accaatactg ccacaataac cattactaag aacaggatta
     2281 ttgcgaacca tacaaaggtt cgtttctgct ctttggtcca tttcgagata tgttcatgct
     2341 gctcgcttgc gctatccgcg actgtctttg caactttgga taccttttga ggggctttcg
     2401 atgcagtttc cttcagtatt tttgtaaaat ctttgatatc tttcttgtct ttcttggcca
     2461 tgctctagaa ttccttcatc tgcaattatc ggcaaatatt ataaccatcg ctgagatgga
     2521 gcctttttcg gaaagctctc taaaagctct ctaatggaga accgcaccaa taatgtctct
     2581 cgctccgcaa gcaaactcct gaaacccaac agccttgcga gtcgccatct gtagtctttt
     2641 taccgcaatg tagccatctc cggtccttat aataattccc ttctgcataa ctaccgcaat
     2701 tatagtcccg ggagaaggat ttgtcacata tccagccttg ctcataggat caaaatcatc
     2761 gaaaggctct gcttctagaa tgcaaaggcg catatctcta agaaaagcaa atgagcctgg
     2821 ccatggatcg aaggcacgta tgcgagcatc gatttcgcgc gatgttctat gccaatcgat
     2881 atggccatct ttctttgtta ttttcctgca ataggtgggc tcccctaact gaggcctgga
     2941 ctctgcaatt ccattttcta tttcatccag gacttcgacg accattgggc ctgcgagttg
     3001 tgcgcagaga tgagaaagag aagcgcttgt ctcccggcca gtcagtggaa ttcgatgagt
     3061 attcaagagc tctccggtgt ccatttcggg agctatcgtc tgtatcgata ttcctgtttc
     3121 ctcatccatg aacattatgg catgaggtat gggcgaactg ccccgccatc ttggcaaaag
     3181 gcttgggtgc acatttatgc cgccttttgg gaaaagctcc ataaatctgg gtccaaaaat
     3241 tcgtccgtag gcaaaagaga caagtatatc gggtagaatt gccaaaactg cggctcgcgc
     3301 ttcggacttg agtgtctcga aagtgagaac cggcacgcta tctccccata agcgtaatgc
     3361 cgcttgcgct acgggagtac tgttcgtcct aagacctcga cctgctttgg tctctgggtt
     3421 cgtcagtatt ccgacaacct catgtgacga atcatacaag ctctctaagc tgggaactgc
     3481 aatatcggga tttcctgcaa ataaaacgcg caccggcgac tcctagtcgt tttacatatt
     3541 cagcatgcgg ccgtagtgtg caagcgctcg ttttcttttg acgggcgata cgcggtctat
     3601 aaataaaata ccatccagat ggtcatattc atgctgaata actctggcca ataagccatc
     3661 tgcgctgatt ctgagaggct taccatgcat atcaacggct tctatatcta cggccaaagg
     3721 acgaaccacg ttgaaatata agcccggaaa cgagagacaa ccttcttcga attctgtggt
     3781 ttcctgactt ctggctatga tgtggggatt gataaaaacg cgaagcggtt cgtcaccgat
     3841 gccaacgata aagagccgcc tgggaattcc cacttgagaa gcagcgagac caattccatt
     3901 gttgcgccgc atgacttcat gcattccttg aatgagctgt tccagactat gatcgaattc
     3961 ggtaacctca tgcgctttct cgctgagaat cgattcacca taggtcaata tattgaacat
     4021 atcaatttcc tatattaggc atatttgcgt ttcaggtata acttaacgca aaacaagact
     4081 tcttggcaat gttccgcgct ttcgttttat tccagggatg gaatccgtac tttatctccc
     4141 actttgatac cagagcgctc gaaccagcct tgaggtactt cgagggcgta tcgtacgctt
     4201 ctaatggagg gtcgagactc ctccgaaaaa ggcacaagat cgataatttg cacaattgtt
     4261 ccatcagaga gaatataggc caacgatagt ggcaacttgg tgtttttcat ccagaaagca
     4321 agcttctgat cgctttcgaa tacaaagagc atgccttctc catcctttag tgagctgcgg
     4381 tacataaggc cacggtttct ttcgccttcc gtaagcgcaa cttcggcctt tatttttgcg
     4441 tttccgattg tgagatcgac tgttttgagc ttaggatttg gtctgtccgt gccttggctg
     4501 gagttgcatg ctgcaaaaag aatgagaatt accataaatg gcataattga taaaccctta
     4561 gacaagtatc gagttggcat ttctgttgcc atagtctacc atttccttta tccataagct
     4621 acttcagact tgagttggcg tacaccactc taaatctgaa gttgtgctgc cttaagcgca
     4681 gccataataa acaactaaaa aaaataaaaa agccgcttag gcagaagcgg cttttctaca
     4741 tgtgtcatac tctagtcctc gaagatcaat atctgccttc ttgtgatctt cgagtttttt
     4801 tcatgagctt ccgttcaagc gctttctttt ctctattgcg aatagcggat ggcttttcgt
     4861 aatactcgcg ccgcttccat tcgcgaatga taccttcttt ttcaaccatt ctcttgaagc
     4921 gctttaatgc cttctcgagt ggctcattat cttcaacagt gatctgagaa atgattctca
     4981 cctcccttct atacgcggaa tgcattgtaa ctgaagcaaa tattttgtca agcgctttag
     5041 tttatatgtt gcagctcttt tgtaaatcta atttgaacga tatactttca gcgaggagtt
     5101 cttagatgct gttttcactt ggaaatctga taacgctagc tattgttatt gcgatgtttg
     5161 tggcctacca tctatggacc gcagataatc gttctctcga aaaactgaaa cgcctgggag
     5221 acaagctcaa ggacgaactt ggctcctatg ttgaatcaaa ggccgaggaa ataaagcact
     5281 atggaatcga tctcgatgtg caacagaaag cggcgaaggt cgtgctggaa aaaatccagg
     5341 aagcccaggc aactctcgat gagaaatcgg attcgatcac cgaaatagcc gactgtttca
     5401 aaaaatacga caatgtttta tcccagctca tggatatgac aaagcgtgtt gatcaaaata
     5461 ttgcacatct tgccgaaaag aatgatttca tccaggcaat cacgcgacga ctcgaagatg
     5521 ctgataaaga aatcgatatc atcgaaaaag aaatgcctaa actcaaagag cagttcgctc
     5581 atgatgctca gcaaattcta gatggatttc gtgacgatat tctcgatcag ctccatgaac
     5641 ggctgacggg aataatcaat ctattcgaaa aatctcggag cgatgcagag tcggcggtga
     5701 ctgaagctgc ttcgctcaaa gaaaatatcc atgagatggt tgaggctgcc ctctctgcag
     5761 cgacatctcg cgcgcagagc atagaggatt cggcctttgc cacccttcgt gcccacattg
     5821 ccaacaactt agaggaactc tcacaccaga ccgatgctcg tatagaaggg cttagccagc
     5881 gcattggtga gcttgtgaat gccataaacg ccaaattgac cactttggcc tcagaaaccg
     5941 catctcaact tgccgagtcc gatcgcaggc tttctgaggc aagagctgcc ttgagcgcaa
     6001 tgacctccga catggcgcgc attcaaaagg aatccgcgga aatcaccgta gcactttcta
     6061 aagaattcaa agccgaacgc agttcgctcg aagaacaatt cgagcagttt ggtcaagctt
     6121 tcgaaattca tcgggcatcc ttcgaaagag agttccttga cgagatatcg gcactacgca
     6181 aatccctgga ggatgcaaag gccaatagcg aagagctcag aagcgcagcc cagggcaata
     6241 ttgccgaggc tctcgatgaa ttggagcgga acgtagccag ggaattggag tcgcgaagat
     6301 cggccttgga tgagcgtata gatgaatggc taaattcgat ggatgctaag atccgcaagg
     6361 tctctcagga tgctctggag cagcgcaagg cagaggaagc ccgcctggct caggaggtca
     6421 atgccgaact taagcgtctc aaagacagtc tctatacaca aacccaaaga atagagcgcg
     6481 atatagaagc ttttaagagc gtctaagaat caagcaaata ttgcgtcgag cgctagttct
     6541 accatctcat tgaaggagct ttgacgttct tccgcgctgg ttatctcgcc ctttacgaga
     6601 tcgtcggaga ccgttagcac agagagagct tcacagccaa acttagcggc caatgtatag
     6661 agctcagcgg tctccatctc tacggctaga acaccaaact ttgcccagag cttccattgc
     6721 tcgggatcct ccgtatagaa catgtcggaa ctcaatatcg aaccagcaaa gactctcagc
     6781 ccgcgagatg tagccgcatc ccaggcggcc ttaagcaggc ggaatgaagc cgtaggcgca
     6841 taatccatgc ccttaaatcg caggtggttc gctgccgaat cggtgcaagc actcatggcc
     6901 agtacaatgt cccggacatt taggtattcg agcatgctac cacaggttcc aatgcgaata
     6961 agcttattta catcatattc acggatcaat tcgttgacat atatggaaag gctgggcatt
     7021 cccattcctg tgcccattac tgaaataggc tttcctctgt aagtacctgt ataaccaaac
     7081 atattgcgca cttcattaaa gcaaaaggcg ccttcgagat aattctcagc gacaaattta
     7141 gctctgaggg ggtccccggg caaaagaatt ctatcggcga tttggccttt ctgcgccgcg
     7201 atatgaatgc tcatatggca gtccttttct atccaagaga ttttacctac tggcttatgt
     7261 attcaatcat aaagccaaac agaactcaat cccttttcag gacggcaaca accgcggcag
     7321 cattttcggc cttaattctt atttcatttg cagcctaata tccttaattc ctatctcatt
     7381 ctcagcctta tattcagtgt attctgaccc acgcttcaga cttgctttat gctctacttc
     7441 cctaagcctt tgcgcgatat ggcaaaagca tagataggcc tcagataggt cgactcgcta
     7501 agcatcaaat cgagcatcac cgcattgaca agtccataat cgccagccag agcctccgca
     7561 aatccggaag catacacctc atttaggggc tctggacctt ccgtggcaaa gcggtaatct
     7621 cctttgtctt gataccataa agttatctgg aatccttcga tgttttgggt atcctcgggt
     7681 tttgtttgat tgtacacggc ctttccgttc tcgggataag tccatgagcc tttttccaaa
     7741 gttacatttg ctttttgtat ttctgagaga aggttttctt tggaggaatc gatggaatag
     7801 atttccttat agcttccatc gggcgaagaa aacacaagcg acttgtttag agttgcaaca
     7861 acatcgcgcg ctcctgatac ctctacccta tccgccttgg tttttattgt ataagaccgt
     7921 ggtttttgct ccgagctcga tgctgctagc gatatagtgt ctcctgaaat actcgcattg
     7981 aattgtgcaa attctctttc tccgctcgct gtacgctggt ataaagtccc tcccactatc
     8041 attcctgatc cgtcagtcag tgcacgaaga attaatgtat cgaagccata ggcttccgca
     8101 tatcccgggt ttgtgtgctt atagactatg aggatattct cattctgctt ctttatctct
     8161 ggcagattcg cgccagcctc aggacgcggc cccggcatgg cggcaatgtc ctgggcagca
     8221 gttttatcgg ttccaccttc tagaggcttc tctactacct tagctttttt gcatgataca
     8281 accataagta ccatagccat cattaagatc aaaaacaagg cgcttgaaac acgaaaattc
     8341 cttctcatac agtacctccc ctcaatactc atgactatac tacattgatt ctgctcagat
     8401 aagagccgaa tctaatttat tacactgtat attattaaaa tccaactcaa aaagccgcca
     8461 tcttctctgt catatacccc ttaggcggat agatggcggc aaagacacat ctcttattcg
     8521 atttttatat cgattttctc cgatctacag atcatctcta tttccaaaca atcttcgcgg
     8581 ctttttcgtg gcaggtgggc ttaccaatat cttctgcacg gcttttggat tctcttctct
     8641 atctttcttt aagaccccgg ctactctgga cagatcatcc tgtttgatta ttaggcatcc
     8701 atgagattta gagacatcga aagaattagt attagagtgt acggcatatc cggtatcttt
     8761 atagacgccg ctggcattat aatttccttt gccatcattg acataagttt cgacttttcc
     8821 aactgcatta gtggaaatca tatatggtcc atatttgtct tcccgaggtt ttactgaagt
     8881 tactgaccaa tagccttccg gaaattgaga aggcttactt gttaccgctc cagatgatgc
     8941 gaatttgcgc ccggttgcat ctgccggcat aaatggcgct ttttgtgtgt ctacgactcg
     9001 attatgaaca tcgatttcaa acacctcttt tggaggaatt cgccccaatc ttgcctcgta
     9061 ctcattatcg atgacattca cttttagttt tgtccgatca cgattcagcg acatacgcac
     9121 atcccctccc gttactgcgg cacactcatc ctgaccaagg ggttcgccaa gattgagaag
     9181 ccgcatacca gggatgatcg cttcaccctg tactgcatag gcgaagggct gcagcatcgc
     9241 tcccaaaagc agacatgcag caattaagat tgctttcttt gtgcgcattt agcacctcct
     9301 cattgtatag acgccaagaa ggtataaata cgcttcaaag tttgattttc tttgaaaaat
     9361 tatctaattt tgcagctaga ctctaaagga tggagattta ccaaagtcca ctgagcaaaa
     9421 gctatgacac ccgtcttaat gatttctatc tcgtacttag aaatacaaca gttagtaatc
     9481 ggaagaagag gcagccaatc gtgaactccg agcgtagatc ttataagcca ttgcaaaaga
     9541 ccgccgtggg taatgcagat tatgcgagaa gctcccctgg tggcgatatc tcgaatatgc
     9601 tgccaagcta taattgaccg tgcgtaaata gatttcgaag attcggctgc gggaatggca
     9661 tcccaacttc gcctatagaa atcctcccat atatctggat atcgctgagc agccgtgctg
     9721 gggtcaatgc cagagaagac accagtgtcc atctcgatga gaatatcgtc gaactcaatg
     9781 tgggctagat ctataactga agagactatt tcggccgatc ttttagctct caacattggg
     9841 cttgctaaga tataatcagg tttgaattgg ctcaaccatt ctcctgcttg cctagcttct
     9901 ttttcgcctt gtgcggtgag cggaaaatca tatcgccctt gataagtatt gctcgcattt
     9961 cctttactct gtccgtggcg aagaataaag acatctagat catgatcaag agtggcaaaa
    10021 aaagcaccat cggcttttac accttgcaat tcttcgatga gatgcgcatc gagaatatag
    10081 cccggctcga atcgcgtata catgttatcc cctggattta aggctcataa tcaatgaggt
    10141 ctcagaagac ctcaagcggc tatgaatgtt tcgcttcctc tattgaagta cttatttacg
    10201 atgaacattg caatacccgt gacaaagaga aggatggtcg acatcgcgct cgctgtgcca
    10261 aaataaccat cagccaccga aatgtagatc tttatgggca ttgtcatggt tttccctaca
    10321 tataggagaa tagaagcaga cagctcattc atagatgtaa cccaactcat tattgcacca
    10381 gatattattc cgctttgcat caagggcaaa gtgatagtcc taaatgttcg ccccggcgga
    10441 gagcccaggt tgataccagc ttcctcgaga gccggatcga tttgcttgag aatagaggcc
    10501 gcagagcgaa ccgagtaggg caagcgccga atgaaataag tcaggatgat tatggccgcc
    10561 gtgcccgtca ggattattgg cggcttgttg aatgcaacta taaacccgat acctagcacc
    10621 gtgcccggaa ccatatatgg aatcattagc aatgggtcga gtatcctaac ggcggcattt
    10681 ctttttctag caattacaaa tcccagaagt gttccaacaa aagcaataag gagcacggct
    10741 gttatggagt atagaaagct atttgtcacg gtcttaggaa catcgaagaa aatctgtcta
    10801 tagctgtcca ggctaaaacc tggcttaaag accggccccg aggtctttcg gaaactaaaa
    10861 tatacaatca cagcaagagg aagagtcgag aaaaagacaa taccatagca caatatatga
    10921 gcgagcaccg attttatccc tttaatctgc tttatcacag gcctattgac cagcatgcta
    10981 gcatatttgc gcctcgaaga gacccactgc tgcaacacga tcacaaccga cgcacagagc
    11041 atcagaataa tcgatacggt tgaagccatt cctggagttt ggctgatttc gctcgtatag
    11101 agattgtagg caagc
//
LOCUS       DAOOWY010000060        11050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_5893_length_11050_cov_9.590814, whole genome shotgun sequence.
ACCESSION   DAOOWY010000060 DAOOWY010000000
VERSION     DAOOWY010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5893_length_11050_cov_9.590814"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..913
                     /locus_tag="PK105_05775"
     CDS             176..913
                     /locus_tag="PK105_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HOU61255.1"
                     /translation="MYKRQSCRKLILVAVFLISIAATATAFTFMPMSITISPSGANSI
                     ASFRITNDSSQQIAITIKAMMREIDENGNETNKPADNLFAIFPTRVVVQPNSFQNIKV
                     QYKGNPSLSREVAYRIIAEQVPIDFSQQQTSGVKVMFRYIAALYVAPPNVSHKVSVTK
                     VEYGEQESKKGFFVTITNSGTRHAIINDPVMKISGSSFTITLKDESVSAINGQNLLAG
                     NVRKFFIPCEDAVPGKNYTGTLTATIE"
     gene            990..3440
                     /locus_tag="PK105_05780"
     CDS             990..3440
                     /locus_tag="PK105_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HOU61256.1"
                     /translation="MLKKKNVYVLVWILFLWLPPIDAQIPQTPIVHDSIVPELEIQFS
                     ISINGKPIGDIETGIWHNEPILNKEIASRLLINYLRPDIYSVIFDTLFKELDWLTAND
                     FSLAGISFLFNELQLSLSIVIPPEYSPVVDIDFVPEQILNYKPILRPEPFAGYIQNES
                     DLHVSDISEGKVLFSTRLNSMIDVKGVHLFSDNFFSYAFGPSQVFSWNMNSVYLLWDN
                     TAIHSRTKIGKISIPAVSNQSQPSLYAMSLESFEDRQYMVRTGYIDNQTEFTIHKTAR
                     VSVEANGRLIRQVILEPGNYRILDLPFTTGLNEFVLRIEEIDGNVQVFRRIIPREASI
                     MQPGTSKYALSAGTSRTDFSEFIASGYYLYGFLSTLSGGINFQVDKRSAMGGLTFSTA
                     LPIGTLNGSGSIVGRWDGWGPVISPAATLSYLFSMPSKIYLPIFGISATFRGEGFLSP
                     NISSPSGTVPSAFLSFSTNFYTKIFANIGIGLSYSLYYTYSSPAKMTHEVFASISTAI
                     AAGGNLGLSGRLSFAESSAPTFSLNLSFTIMPKDDTTRVLNFMQSSGGQTNISINDKL
                     YMLGRVFDTNFGASDLLPGASKDGSMSLGIRNSSEYFDVSANGTFNYSPSSESYSLGG
                     YTQFRSSMAFAGTHVAFLRQIPDSFLLITASPELKDKDIVYRVSNGSQYQAAGGKNIV
                     VPIQSYETTILSIDLRATELNVAPRYSFITLSPAFRSGILFESDVIKRYFINGRLINQ
                     EGKPISYLPGDVFDFGGSLVTSTFTDELGDFEIYDILPGSYQIQWPEGFGTTQFELPD
                     TDEDRIDLGDIKLHIESK"
     gene            3467..4384
                     /locus_tag="PK105_05785"
     CDS             3467..4384
                     /locus_tag="PK105_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HOU61257.1"
                     /translation="MRRRFWLFLIICFAIVISMSAEKLTLDPISAPSPVTYDPFSGND
                     TRGQAFTFTVRMDKSTGTTSNFSVVINGESSPSSRSLKSSSGSIEIGFYKDSSHTTEI
                     RSTADYSPSYYISGSFDPKPDVLTQTFTIYPRLVKGQSAPYGTYTGTFTVLLYKKSSP
                     GQGNPEDTISFTYTAAVDKAVDVRVGSRNANYDTGATSYNIDLGEISNGASADFGIFI
                     RGTTGYTLTMHTASGGFLTSSNSEDKIQYTIIVDGKEYYLNSSIILDQETANCLYSKA
                     LLGTIKIPGDHGAKAGIYSDTVGFSMAVN"
     gene            4475..6118
                     /locus_tag="PK105_05790"
     CDS             4475..6118
                     /locus_tag="PK105_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOU61258.1"
                     /translation="MKLLVLAAGIGSRFGGAKQIIEVGPNGETLLEYSIYDARKAGFD
                     EIIFLIRPDIEADFRSNILSRLPSDLRYSLAYQTLDFLLDKALAEINGEKQLDPRELG
                     QREGGLAESKRVKPWGTAHALLCAGELFKQGEAFAVINADDYYGRRAFEAVGSNLFNN
                     PGELCFAGYRLEDVVPASGTVSRAICTLSGDGYLEKIIEHKKVWREGKRFFSEQGESI
                     VELSPDAAVSMNFWGLDYSIFEYAWQGWKEFLSRYIESPTQEFLLPDLIQSIVEKKRA
                     RVRMLPASERSFGLTNPEDLQEVRRRIFGFVAEGVYPSPLFGSWKIKSFPRLWHFADN
                     EYGECAEFEDLFMQAAMPWDVLKLLDSYLRSRLDAVSGEKIRSSIPKGVHIEGDVFID
                     EGCIIEEGAFIRGPAWIGKGCEIRSHCYIRGGLLAGSGVVLGHASEFKHCILLERAQA
                     PHFNYVGDSVLGHHAHIGAGVILSNFRLDGKPIRAKCLDSREKFETELTKFGALIGDN
                     CEIGCNSVLNPGTILGERSIVMPMSLVSGTWPLESLIDI"
     gene            complement(6167..6982)
                     /locus_tag="PK105_05795"
     CDS             complement(6167..6982)
                     /locus_tag="PK105_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOU61259.1"
                     /translation="MPLPIVSQKLKDEALFKPLDFLAYLDKAGIALSQRPAPQTIVLS
                     YQKSLCDFVRATFPCERAKGYFGRFVHYVDVSDMDDSRDKHSPIGNKTIAIASDFGVG
                     APAAAVMLEELIAWGVKEFISIGFAGSLREDVPPGTLVVCDGAFRDEGTSYHYLDSNM
                     SVHPEPRLTEALENSLKRAGLAYAKGPTWTTDAIYRETKSEVQYFRDQGALVADMEAA
                     ALFAVAQYRNVSIASCFSVSDTLAYLEWRPEFHAEPSMQGLEMLFSCAIKALQ"
     gene            7182..8069
                     /locus_tag="PK105_05800"
     CDS             7182..8069
                     /locus_tag="PK105_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOU61260.1"
                     /translation="MHNVVSKVQLSEEVFRIEVEAPLVARERKPGQFVIVMCDDEYSE
                     RIPLTIADADPDKGTVTLIFQTVGASTHKLALKNPADKIILLGPLGNPTHIECYGWTV
                     CVGGGIGSAPLYPIAKALRQAGNRVTVISGARTKDLLIMQDELRAIADEYIVVTDDGS
                     FGRKALVTEPLDELCRRAPPPDLVVAIGPPIMMKFCAETTRPYGIKTLASLNTIMLDG
                     TGMCGGCRVSIGGKTKFVCVDGPEFDAHLVDWDGMMTRLGTYRDIEKEAHDRCHLELQ
                     LEKLDAAEISASRPKESAQ"
     gene            8090..9544
                     /gene="gltA"
                     /locus_tag="PK105_05805"
     CDS             8090..9544
                     /gene="gltA"
                     /locus_tag="PK105_05805"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOU61261.1"
                     /translation="MEEAELELSRLRSGPITPKMRMQIPPQAMPAQDPIERRSNMNEV
                     ALGYSSAQAMLEAERCLQCKNAPCIAGCPVRVDIPGFIGKIKEGDFLGAARIIKRTNL
                     LPSICGRVCPQETQCQAPCTVGKALHGVEKAVQIGRLERFAADYERSRGAVEIPHVAQ
                     KTARKVAIVGSGPAGLTCAVDVRREGHSVAIFEAFHKPGGVMVYGIPEFRLPKVLVQA
                     EAEVLESMGVDFELNFLVGRTRPLLSLIKEDGFDAIFIGTGAGLPKFMDIPGENLVGV
                     FSANEYLTRTNLLKAYLKGRAGTPIYYSRRVAVLGGGNVAMDSARMALRLGAEEVRVL
                     YRRTRSEMPARAEEVEHAMEEGIILDFLKSPTRVLGDDRGRVAALEVLSYELGEPDES
                     GRRKPVPIHGSEEIIPFDTVIVAIGNESNPLISKTTPELQVDRHGHIIVDDRQRTSID
                     RVYAGGDIVLGSATVILAMGEGRRAAQSINEIIQ"
     gene            9564..10271
                     /locus_tag="PK105_05810"
     CDS             9564..10271
                     /locus_tag="PK105_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61262.1"
                     /translation="MSQGRRHPLLAGYYWILLFLGWITIAILPLGAQDSYSAALSLFW
                     DGRRLEMAETRKAESQLAFQRSLAMTERLLSKEPSNPDLQTLKTWNLFRLHRYVDTVV
                     YAQSVLNTREDHRVLETMAEALFFLNKNEEALSAFGRYFTIAPENDDRRSSAFYYVGE
                     IYFRIKKYEHADIAFSTAVSMEKNMYYWWYRLGLVKEILGQYRRAYEAFNTSLSLKSN
                     FKPALEARERVKAKSSL"
     gene            10351..10962
                     /locus_tag="PK105_05815"
     CDS             10351..10962
                     /locus_tag="PK105_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU61263.1"
                     /translation="MYKAVIFDFGNVLCGVERENFVLKAAIHAKAQNAQDLLRTLWGT
                     PIEYEFETGIIDSHEYYRRVQRLVELEPGYSYEEFVRDYQSIIVPNPDGEQALKTAAS
                     MGARTFVLSNTSFLHASLIFDNEVLGTYPEIYIFSYKIGVMKPDPRIWMKLLEYIRLD
                     PGDCLYIDDIADYCKAAQDLGMDAFCYDFRIHNLSLELQNMLQ"
BASE COUNT         3006 a         2443 c         2631 g         2970 t
ORIGIN      
        1 gcgaccccca gcgtgtcata ctggtgctct aaccaactga gctaaccgcc cgctgcatgc
       61 cgcgaacgac agaagctctt ataccagcag atagagagat tgtcaagcga tctcggaatg
      121 caattgcact tatcctcttt tacatataga ataataaaaa tagtgggagg aatgcatgta
      181 taagagacag tcttgtcgta agctcattct ggtcgcggtc tttctaatct caatcgcggc
      241 aacggcaaca gcatttactt tcatgccaat gtcgataacc atatcgccca gcggtgcaaa
      301 cagtatcgcg agttttcgaa ttacaaatga cagttcgcag cagatcgcga taaccattaa
      361 agctatgatg cgagaaatcg acgagaatgg caacgaaacc aataagcccg cagataatct
      421 attcgctatt ttcccgactc gcgttgtcgt acagcctaac tcctttcaga atatcaaggt
      481 acaatacaag ggtaacccat cgctttcaag agaagtcgct tatcgcatta ttgcggagca
      541 ggtacccatc gactttagcc agcagcagac ttccggcgtc aaggtgatgt ttcgatacat
      601 tgcggctctt tatgtagccc cgccaaatgt tagccataag gtaagtgtca caaaggttga
      661 gtatggagag caagaaagca aaaagggttt ctttgttaca ataaccaata gtggtacgcg
      721 tcatgctatt atcaacgatc ctgtaatgaa gatctctggc tcctctttta caatcacgct
      781 gaaagatgaa tctgtcagtg caatcaatgg tcaaaacctg ctggctggca atgtgcgaaa
      841 attctttatt ccctgcgagg acgcggttcc tggcaaaaac tatacaggca cgctgaccgc
      901 aacaatcgaa tgaatctctt atgcagttct acatgatctt tggaagcgct gagcctcaag
      961 gctttttgta aggccagagt gagcaagaaa tgttgaagaa aaaaaatgta tacgttcttg
     1021 tttggatttt gtttttatgg ctccccccta tagatgcgca aatcccccaa acacctatcg
     1081 ttcacgatag cattgtgcca gaactggaga tacaattctc catcagtata aatggcaaac
     1141 cgataggaga tatagaaaca ggaatatggc acaatgaacc tattctcaat aaagaaatag
     1201 cctcgcggct tctcataaat taccttcgtc ccgatatata ttcggttata ttcgacacgc
     1261 tattcaaaga gctcgattgg ctcacggcca atgatttttc tcttgctggt atttcctttt
     1321 tatttaatga attgcaactt agtctttcga ttgtgatccc gccggaatac agccctgtgg
     1381 tcgatatcga ctttgtgcct gaacaaattt tgaattacaa gcctattctc cggcctgaac
     1441 cttttgcagg ctatatccag aatgaatcgg acttgcatgt ctctgatata tcggaaggaa
     1501 aagtcctatt ttctacacgt ctcaactcga tgatcgacgt aaagggggtt catctcttct
     1561 cagacaattt tttttcatat gcgttcgggc cttctcaggt tttttcctgg aacatgaact
     1621 cggtttattt gctttgggat aacacagcaa tacattcaag gacgaaaatt ggaaagatca
     1681 gcatcccggc agtgagcaat caatctcaac catcgcttta tgcaatgtct ctcgaaagct
     1741 tcgaagatag acaatatatg gtacgcactg gatacataga caatcaaacg gagtttacga
     1801 tccataagac tgcaagggtg tctgtagaag ctaatggaag gcttattcgg caagttattc
     1861 tcgaacctgg caattatcgg attctcgatc tgccctttac aacagggttg aatgaatttg
     1921 ttctgcgcat agaagagata gatgggaatg tccaggtatt tcgtcgcata attccgagag
     1981 aagccagtat aatgcaaccc gggacatcga agtatgctct ttccgctgga acatcgcgta
     2041 ccgacttcag cgaatttatc gcctcaggtt attatctata tggctttttg tccactcttt
     2101 cgggcggcat aaactttcag gtcgacaagc gctccgcgat gggtggactg actttttcta
     2161 cagcactgcc aatcggcaca ctgaatggtt ccggctctat tgtagggcga tgggatggct
     2221 ggggacctgt tatttcacca gcggcaacgc tgagctatct cttttccatg ccttcaaaga
     2281 tatacctacc catctttgga atctctgcca catttcgagg agaaggattc ttatctccta
     2341 atatatcttc tcccagcgga acggttccca gtgcttttct ctctttctcg accaatttct
     2401 acacgaagat ctttgcaaat attggcatag gattgagcta ttccctttat tatacatact
     2461 catcgcctgc taaaatgact catgaagtct ttgcttcaat cagcaccgca attgcggctg
     2521 gcggaaatct tggtctttca ggacgccttt ctttcgcaga atctagtgca ccaacgtttt
     2581 cactaaattt gtctttcacg ataatgccca aagacgacac aacgagagtt cttaatttca
     2641 tgcagagttc cggaggacag acgaatatca gcatcaacga taagctctac atgcttggca
     2701 gagtttttga tacgaatttt ggcgcaagcg acttacttcc tggtgcatcg aaagatggct
     2761 cgatgagtct gggaatacga aattcatcag aatatttcga tgtaagcgca aatggaacct
     2821 tcaactattc ccctagctcg gagagctatt ctctgggtgg atacacccaa tttagaagct
     2881 cgatggcttt tgctgggact catgttgcgt ttttgcgcca gataccggat tctttcctcc
     2941 tgattacggc ttcgccagaa ttgaaagaca aggatattgt ctatagagtc agcaatggat
     3001 ctcagtatca ggcagcaggc ggaaaaaaca tcgtcgttcc tattcaaagc tacgaaacaa
     3061 caatactatc gatcgatctt agagccactg agctgaatgt tgctccgcgc tactccttta
     3121 ttacgctctc ccctgctttc aggagtggta ttctattcga aagcgatgtt atcaaacgat
     3181 atttcattaa cggtagactt atcaatcaag aaggcaaacc aatttcatat ctgccgggtg
     3241 atgtcttcga ttttggaggc tcgcttgtga cttctacctt tactgatgaa ctgggggatt
     3301 ttgagatcta tgatattctt cccggaagtt accaaataca atggccagaa ggctttggaa
     3361 ccactcagtt cgaattgccc gacaccgacg aggatagaat agacttaggt gatataaagc
     3421 tacacatcga gagcaaatag gactataatg aataaaggaa tagcatatga gaagaagatt
     3481 ttggttattt ttgataattt gtttcgctat cgtcatttct atgagtgcgg aaaaattgac
     3541 cttagatccg atatctgctc cgagtcctgt tacttatgat cctttttctg ggaacgacac
     3601 tcgtggccag gcgtttacct ttacggtgcg aatggataaa agcacaggca ccacaagcaa
     3661 tttttcagtt gttataaatg gagaatcgtc tccctcttca agaagtctta aatcatcttc
     3721 ggggtctata gaaataggct tctacaaaga ttcctctcat acaacagaga tcagaagcac
     3781 tgcagattat tcgccttctt attatatctc aggatccttc gatccaaagc cagatgttct
     3841 tactcaaacc tttacaatat atcctcgcct tgtcaaaggc caaagcgcac catatggtac
     3901 ctataccggg acttttactg tattacttta taaaaagagt tccccaggac aaggcaatcc
     3961 ggaagatact ataagtttca cttacacagc agcagtcgac aaagcagtag atgtgcgcgt
     4021 tggttcgcgg aatgccaatt atgataccgg agcgacttca tacaatattg atcttggcga
     4081 gatatcaaat ggcgcaagcg ccgattttgg gattttcatc aggggaacaa ctggatatac
     4141 cctaacaatg catacggcct ccggtggatt tcttacctcc agcaattctg aagacaagat
     4201 acaatacacg ataatagtcg atggcaaaga gtattatctt aattcaagca taattctcga
     4261 tcaagagacc gctaattgtc tttatagcaa ggccttattg ggcactatca agatacctgg
     4321 cgatcatggt gctaaagcgg gaatatactc ggacactgtc ggtttttcta tggcagtcaa
     4381 ctgattcttg tcaactaatc cttgctgcca taaacaaggt tcgtagcagt atttgcggct
     4441 tgaccagggc catcagaatg cgcatcattc agccatgaaa ctattggtac ttgcggccgg
     4501 tatcggctcc agattcggtg gagcaaagca gattatagag gttggtccta atggagagac
     4561 cttactcgaa tactccatat atgatgctcg aaaagcaggc ttcgacgaaa ttatatttct
     4621 gattcggcca gacatcgagg ccgatttcag atcgaatatc ctttcgcgct taccctcgga
     4681 tctacgctat tcgctcgcct atcagacact cgacttccta ttggataagg ctcttgccga
     4741 gatcaatgga gagaagcagc tagacccaag ggaattaggc caaagggaag gtggccttgc
     4801 agagagcaag cgtgtcaaac cctggggaac ggctcacgcg cttctctgtg ctggagagct
     4861 tttcaaacaa ggtgaggctt ttgcggttat caatgctgac gattactatg gccggcgtgc
     4921 attcgaggca gtaggctcaa atctatttaa taatccagga gaattatgct tcgctgggta
     4981 tcgattagaa gatgtcgttc cagcttctgg aacggtatcg cgagcaatat gtaccttatc
     5041 aggcgatggt tatcttgaaa agataataga acacaagaag gtatggcgtg aaggaaagcg
     5101 ctttttttcc gaacagggcg agagcatagt tgagctgagt cccgatgctg ctgtctccat
     5161 gaacttttgg ggcttggatt attcgatttt cgaatatgca tggcaaggct ggaaggaatt
     5221 tctgagtcga tatatcgaat ctcctacgca ggaattcctg ttgcctgatc tgatccagag
     5281 tatagtggag aaaaagagag cacgagtacg catgcttcct gcgagcgagc ggagttttgg
     5341 ccttacaaac ccggaagatt tgcaagaagt aaggcggcgg atcttcgggt tcgtagcaga
     5401 aggggtttat ccatcgcctc tttttggtag ttggaaaatc aagtcatttc ccagactatg
     5461 gcactttgca gataatgaat acggagaatg tgccgaattt gaagatctgt tcatgcaagc
     5521 cgccatgccg tgggatgtgc tcaagttact tgattcttat cttcgatcta gactcgatgc
     5581 cgtatctgga gagaaaatcc gatcctctat tccaaaaggc gtacacattg aaggtgatgt
     5641 tttcatagac gagggttgca ttatagaaga gggggctttt atccgcggac cagcctggat
     5701 tggaaagggt tgtgaaatcc gaagccactg ctacattcga ggcggattgc tcgctggttc
     5761 aggcgttgtg ttggggcatg cgagcgaatt caagcattgc attctcctgg aaagagctca
     5821 ggcaccacac ttcaactatg tgggagattc tgtgctgggg caccatgcgc acataggagc
     5881 aggagtcatt ctgtcgaact ttagactcga tggaaagccc attagggcaa aatgtctaga
     5941 ttctcgagaa aagtttgaaa cagagcttac caaatttggc gcacttatag gtgataattg
     6001 cgaaattggc tgcaattcgg tattaaatcc aggcactatc ctgggagaga gaagtatcgt
     6061 tatgcccatg tctcttgtat ccggcacatg gccgctagag agtctgatag acatctaaat
     6121 atgaattttc catattgccc ggtgttattg cattagctga acaacattat tgtaacgcct
     6181 ttatggcaca agagaacagc atttcgagcc cttgcatgct tggttcagcg tgaaactcag
     6241 gcctccattc gagataagcc aatgtatctg atacagaaaa acaagaagct attgacacat
     6301 tgcgatactg cgctaccgca aagagtgccg ctgcttccat atccgccaca agggctcctt
     6361 ggtcgcggaa gtactggact tctgacttag tctcgcggta aatggcgtca gtggtccatg
     6421 ttgggccttt ggcatatgct agtccagctc tcttgagcga gttttcaagt gcctctgtga
     6481 gtctcggctc ggggtgtacc gacatattgc tgtctagata gtggtaggaa gttccctcgt
     6541 cgcggaaggc gccatcgcag accactagtg ttccaggcgg aacatcctcg cgcaaagaac
     6601 cggcaaatcc aatagagatg aattccttta ctccccatgc tatgagttct tctaacatga
     6661 ctgcggcagc cggtgctccg acaccgaagt ctgatgcgat agcgatggtc ttattgccga
     6721 tcggcgagtg cttatctcgt gaatcatcca tatctgatac atctacataa tgcacgaagc
     6781 ggccaaaata gcctttggct cgctcacaag gaaatgtggc gcggacaaaa tcgcagagac
     6841 ttttctggta acttagaacg atggtttggg gcgccggcct ttgggatagg gcaataccgg
     6901 ccttatccag gtaagccaga aaatcgagag gtttgaatag agcttcgtct ttaagctttt
     6961 gggatacgat tggcaggggc atcggtactc cttcttcttc ttataatcgg tcttatattg
     7021 tactattctc ttcttaagat ggagtattgt ataggtatat tcaaggtata gctgcaaaaa
     7081 gcggagatta tagaaggaat aagcacagag agaataatct ggtttatgga caaaaatcac
     7141 taatccccgc taaaatacat gcggagttga gaatgagaag catgcataat gtagtttcaa
     7201 aagtacagct ttcggaagag gtcttccgaa tcgaagtaga ggcacctctt gttgcacgag
     7261 aacggaagcc ggggcagttt gtcattgtta tgtgcgatga tgagtattcg gaacgaattc
     7321 cacttactat cgcagacgca gatccagata aaggaacagt gactctgatc ttccagaccg
     7381 ttggtgccag cacgcacaag cttgccctca agaatccggc agacaagatt atcctgctcg
     7441 gtcctctggg aaatccaacc catatcgaat gttatggctg gacagtatgt gtgggcggcg
     7501 gcataggatc agctccatta taccccattg ctaaagctct aaggcaagcg ggaaataggg
     7561 ttacggtcat ttcgggggct cgaacaaagg atcttctcat aatgcaggac gagctcagag
     7621 caattgcgga tgaatatatt gtggttacag acgatggctc cttcggacga aaagctctgg
     7681 tcacagaacc gctcgacgag ctctgtagga gagcacctcc gcctgatctc gtggtcgcta
     7741 taggcccgcc tattatgatg aaattctgtg ctgaaacaac caggccttac ggtatcaaaa
     7801 cgctggcctc tcttaacacg ataatgctcg atggcacagg tatgtgcgga ggttgccgcg
     7861 tatccattgg cggaaagaca aaattcgtat gtgtggatgg ccctgaattc gatgcccatc
     7921 ttgtcgattg ggatggaatg atgacccgtc ttggaacata tcgagacata gaaaaagaag
     7981 ctcacgatcg ctgtcatcta gagctgcagc tcgagaagct cgatgcagca gagatatcgg
     8041 cgtctcgtcc caaggagtct gcacaatgag ccataaagaa gaatctacaa tggaagaagc
     8101 tgaactcgaa ctttcccggc tgcgctccgg accaattacc ccaaagatga gaatgcagat
     8161 acctcctcag gcaatgcccg ctcaggatcc tatcgagcgc cgcagcaaca tgaatgaagt
     8221 agctctgggg tacagttctg ctcaagctat gttagaagca gagcgctgtc tgcagtgtaa
     8281 gaatgctcca tgtatcgcgg gttgcccagt gagagtcgat attccagggt ttatcggcaa
     8341 gatcaaggaa ggagatttcc ttggcgccgc cagaataata aagagaacca atttgcttcc
     8401 ttcgatatgt gggcgcgttt gcccccagga gacgcaatgt caggcgccat gcacggttgg
     8461 caaggctctt catggtgttg aaaaagcggt tcagataggc aggctcgagc gctttgccgc
     8521 agattatgaa cgcagcagag gcgccgtaga aatacctcat gttgctcaaa agacagccag
     8581 aaaggtagca attgtcgggt caggccctgc aggccttacc tgcgcagtcg atgttcgaag
     8641 agaaggccac agtgtcgcga ttttcgaagc cttccataaa ccaggaggcg ttatggtcta
     8701 tggaattccg gagttcaggc ttcccaaagt cctcgtgcag gctgaagcgg aggtcctaga
     8761 atcgatgggg gtcgattttg agctcaattt cctggtcgga agaactcgtc cgcttctttc
     8821 cctaattaaa gaagatggtt ttgatgctat attcataggc acaggggcgg gcttacccaa
     8881 atttatggat atcccgggag aaaaccttgt aggagttttc agcgcaaatg aataccttac
     8941 gcgaactaat cttcttaagg cttacttgaa aggtagggct ggcaccccca tctattattc
     9001 aaggcgtgtc gctgttttgg gcggaggcaa tgtggccatg gactcagctc gcatggcgct
     9061 aaggttagga gcagaggaag tacgtgttct atatcgacgc actcggtcag aaatgcccgc
     9121 acgggctgag gaagttgaac atgccatgga agagggaata attcttgatt ttctaaagag
     9181 cccgacgcgt gtccttggag atgatagagg aagagtcgcc gccctcgaag tgctgtctta
     9241 tgaattggga gaaccagatg aatccggcag aagaaagccg gttcccattc atgggagcga
     9301 agagataatt ccatttgaca cggtgatagt agccattggc aatgaatcca atcctctgat
     9361 ttctaagact actcctgagc tccaggtaga tcgtcatggt catatcatag tcgatgatcg
     9421 acaaaggacc agtatcgatc gcgtctatgc cggcggagat atagtgctgg gttctgcgac
     9481 cgtcatactc gctatgggcg agggtaggcg tgccgctcaa tctataaatg agattattca
     9541 ataaaggaga ggaggcgagt ggagtgtcac agggcagaag gcatccttta ctagcaggat
     9601 attattggat tcttcttttt cttggatgga taaccatcgc aattctgcct ttaggagcac
     9661 aggatagtta cagcgcagcc ctatcacttt tttgggatgg tcgccgactc gaaatggcgg
     9721 agactcgcaa agcggaaagt cagctcgctt ttcagcgttc tcttgctatg acagaacgct
     9781 tattatctaa agagccttcc aatcctgatc tgcagaccct caaaacctgg aatctgtttc
     9841 gtttgcatcg atatgttgat acggtcgtat atgcccaatc tgtgttgaat acaagagaag
     9901 accatcgtgt ccttgagacg atggccgagg cgctcttttt cctcaataag aatgaggaag
     9961 ctctatctgc cttcggtaga tattttacaa ttgctccaga aaacgacgac cgacgctcca
    10021 gcgcatttta ttatgttggt gaaatttatt tccggataaa gaaatatgag catgcggata
    10081 tagcattttc gacagctgtt tcaatggaga agaatatgta ttactggtgg taccgcctcg
    10141 gacttgtaaa ggaaatcctc ggccagtatc gacgcgctta cgaggccttc aatacttctc
    10201 tttctctaaa atctaatttc aagcctgcgc tcgaggctag agagcgagta aaagcaaaat
    10261 cttcacttta agttcagttt tcaaaattac aatgtgaacg gatcttatga agtattttct
    10321 agggaatcat taatttttta aggacttgcc atgtataaag ctgttatttt tgacttcggc
    10381 aatgtgctat gcggtgttga gcgagaaaac ttcgtgctga aggccgctat ccatgcaaaa
    10441 gcacagaatg ctcaggatct tctgcgtacc ctctggggta cacccatcga atatgagttc
    10501 gagacgggta ttatcgattc ccacgaatac tatagacgcg tgcagaggct tgtagagctc
    10561 gagcccggct atagctacga ggaatttgtt agagactatc aatccattat cgtgcctaat
    10621 ccagatggcg aacaagctct taagaccgca gcaagcatgg gtgccagaac ttttgtttta
    10681 tccaatacct cttttcttca tgcttcgctg attttcgata atgaagtgct gggtacttac
    10741 cccgaaatat atattttttc ctataaaata ggagtcatga agccagatcc tcgtatctgg
    10801 atgaagctgc tggaatacat tcgccttgat ccaggggatt gtctatatat cgacgatatc
    10861 gcggattact gcaaagccgc gcaagatctc gggatggatg ctttttgtta tgattttcga
    10921 atacataatc tctcacttga actgcaaaat atgttacaat agataaagcg aacaaaatgc
    10981 acattgcggc gcgacgggtg tctagaatgg cagccgcagg tacaagagga ggttctatga
    11041 aacgatttgt
//
LOCUS       DAOOWY010000061        11024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_5915_length_11024_cov_12.318899, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000061 DAOOWY010000000
VERSION     DAOOWY010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5915_length_11024_cov_12.318899"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..3166)
                     /locus_tag="PK105_05820"
     CDS             complement(161..3166)
                     /locus_tag="PK105_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOU61264.1"
                     /translation="MYFNELVSTYDSQRRERNIYHDLMRDRVREILLVGSLYDSFVVE
                     SDGVLTEQIYGEYFKLNLNAVPRITCAYTEESAMDLFRDGRFDLVVVMASTEFDMPLR
                     LAVSMKSTWPDIPILLMVTDNSSLAMLDMSRPELAVFNRIFVWNGYSKLFVGMIKYIE
                     DWRNARADTHAGQVRVILLIEDSVRYYSRYLPVLYRVVLKQTQALIEGERIAETNKLM
                     SIRARPKVLLANTYEDALEVFESYKPYLLTVITDIRFRRNGEFDDNAGFDFLKFVKQE
                     LPDLPMLMQSSETNVREKAYAFGASFIDKNSESLEMELAAFFEANLGFGNFVFRMPDG
                     AEIGQAGNMSEFIQKLNEIPIESLLYHAGHNHFSAWLMARGEINVAKIMKPYRITDFG
                     SPEELRQFIVNMIDQIRSVHSRGIVPELDPEMVKHSRYLCKIADGSVGGKGRGLLFIR
                     SLLENFDFPQYVPGIEISMPNTVFIGIDEFEHFLESNGLWSWAYYDKEVQDLRSVFVQ
                     KPLSQKLENNLRQLLSLSKRPLAVRSSGLFEDMLMVPFSGVYDTYILPNNHPDSEVRF
                     KQLSTAVKLIYASLFSKEARAYFEATNYNLEEERMAVIIQELAGNRHGDYFYPHISGV
                     AQSYNYYPISYVKPDDGLCIAALGLGTYLVTGGTAFRFCPRYPKLDIGPLEYISENTQ
                     RYFHVLSLRDKDPNLLKGEMASLFELPIDAAESDRYFSMFASTWDSLDQRFVPGVSTD
                     GHRIIDLANILKHEAYPFAKAIDIALDIGKRSMGTPVEIEYALNFDDDKQEPTLYLLQ
                     LKPLIHLEDQIEIDLSSIQKDACFLLSRNCMGNGRDLSIHNIVWVDPRKFQRNETLEI
                     AAEIEELDVLARKENFDYLLIGPGRWGTRDRWLGVPVSFDQISHAKVIVEMDLPGFVV
                     DSSLGSHFFHNLTTMRIKYMKINQGSADDHIDLEWLYSLKPRVGTRHCVLTELNTSLD
                     MRFDGRSGKGAIFKSTESSNLKRAIETGN"
     gene            3300..4241
                     /gene="aroF"
                     /locus_tag="PK105_05825"
     CDS             3300..4241
                     /gene="aroF"
                     /locus_tag="PK105_05825"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOU61265.1"
                     /translation="MFKNIKLAARNPGQKTTVIDISGVKVGDDLVVIAGPCSVETEKQ
                     TIETAIAVKNAGAKMLRGGAFKPRTSPYAFQGLGLKGLKILDKARRETGLPIVTEVVD
                     TRDVSWVGEYADVLQIGARNMQNFSLLREVGKSGKPILLKRGMYSTLEEWLNCAEYIL
                     AEGNPNVILCERGIRTFEAYTRNTLDLSMIPAVHRESHLPIFVDPSHGTGILSLIEPM
                     SLAAVAAGVDGLEIEVHIDPKSALSDKDQQLTTVMFESLMKKLRGLKHFLDESNVASD
                     ESSDEYASEECISSSHDRTKASNSVGTLTDAFSEAVD"
     gene            4252..5310
                     /locus_tag="PK105_05830"
     CDS             4252..5310
                     /locus_tag="PK105_05830"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOU61266.1"
                     /translation="MQRNQSIIRFGKTSELLSLIPIEKAIFVTSEALYGQALEMLSSS
                     SLDAKEGLCWIKVPDGEACKNFSVLETVCRQMIDLDANPDTVLVAIGGGSLSDLVGLT
                     AHLWMRGIDIALVPTTLLAMIDASIGGKNAIDIGPTKNLIGSFHIPRLILCDVGWLET
                     LPSRDLLSGMAEAIKHAVIDSEEHFRFLEGIANSEESLTEINAELWEELVQRSQQVKI
                     RYVDADPYDNYLRHALNYGHTFGHAIELITEIPHGFAVATGIGMANDLAVSRGALDPD
                     IAARINSLLHSIGLPANVAEVSAIAGCNIRPAAIYDLIRADKKRRGESIDLALLRNIG
                     DIRIEPVALQYIAEFLGA"
     gene            5338..6360
                     /locus_tag="PK105_05835"
     CDS             5338..6360
                     /locus_tag="PK105_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOU61267.1"
                     /translation="MNTIGKIFCISLYGESHGKAVGVVIDGCPAGISLTEEDFAEDLN
                     RRKAGAEGTTTRTESDKPILLSGVFDGHTNGAPIHIAFRNENIRSSDYEQFIDIPRPG
                     HADFSAMMKFQGWQDPRGSGHFSGRVSIGLVAAAVVAKKILNSFSDIVFRTRLLEAGG
                     SRDIDKAVYQAKESGDSIGALVEIRVSGVPAGWGEPFFDTAESIISHYIFAVPAVRGV
                     EFGDGFAAAKMRGSQHNDPFIDLHGRTARNGAGGINGGITNGNDILLRVAVKPASSIA
                     STQNTLDFSRDEMVNLEIHGRHDACIALRGAVVLEAATACALADLALVARAEAPFISN
                     IPWRKS"
     gene            6357..7415
                     /locus_tag="PK105_05840"
     CDS             6357..7415
                     /locus_tag="PK105_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOU61268.1"
                     /translation="MNLDDIRSDIDRIDAKILSLLNERMEKSLIARHFKAVALDPVRE
                     QIVLEKVRRSSQCLLDPHFSVHLYEQIISECRHLQDAGLKTVAFQGEHGAYSEVALRL
                     LMPTAATIPCCEFSDVFDGVEKGIYDYGIVPVENTLGGIVGPVNSILIYTRLKIVAAI
                     DLPIRHCLLTIPGADHRELRIARSHTQALTQCRNFLLRNHLDPEPFFDTAGAAKALAE
                     ERPKGVSVVASKFAADLYGLEIIKEDIQDSPHNRTRFFIIATEENGVEGDKCSAVFTA
                     GNKAGALFSVLEVFAEENINLTRIESVPDVPEKYAIFIDFEGSLSSAPVQRALEKVPE
                     IAEDFKILGCYHEMRVES"
     gene            7412..8188
                     /locus_tag="PK105_05845"
     CDS             7412..8188
                     /locus_tag="PK105_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOU61269.1"
                     /translation="MRIAILGAGRMGAWLTEELCWNNEVMVHDSDLLKMKYFIKVHRA
                     LAIEEFADFKPELFINCVPLGLTLDSFDAVLPYLPSQCIISDIASVKTGFREYYENCR
                     HPFVSSHPMFGPTLANIRDLHEESAIIISESSKEGADFFRRFYSSLRICIFEYSFEEH
                     DKTVAYSLATPFASTMVFAACMKKQDAPGTNFKRHLAIAKGLLSEDDRLLTEIMFNPY
                     TIRQLELINSKLAYLTHIIRQRDYDEMKKFLDKLRANIEQ"
     gene            complement(8294..9634)
                     /gene="aroA"
                     /locus_tag="PK105_05850"
     CDS             complement(8294..9634)
                     /gene="aroA"
                     /locus_tag="PK105_05850"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOU61270.1"
                     /translation="MGTRVAKPGKVCGSIQAPASKSALQRAIACAILARGMSHIEASP
                     VCSDARAAIIIAKELGAKVKEEKGILEIEGSPLFHKDAKGKLATTSQKVCYPGLKNNG
                     FQPCLPLVLPCGESGLCMRMFSPIVALLDREVELRGEASLSSRPMNMVEDSLKSLGIS
                     CISRGGVQPIRIQGPLHPLKSRIDALGSSQFITGLLMALPLLDGDSELIVDNLVSSGY
                     LDLTIEICARFGVEIKRNANGAGFWIPGNQVYKATDLIIEGDWSAGAFLAVGAAIAGS
                     EEGLEIHGLHQDSTQPDRAIVDVLTEAGAQVQFKGSDCIVRASALAPFDFDATNCPDL
                     FPPLVAMAAAIQGRSCIRGTKRLLSKESNRSVALCKTFRKLGVDVTLQNDAMYIVGGS
                     IHGATVESYGDHRIAMAASIAALVASGEVSIEGAECVSKSWPGFFDDLSSIVVS"
     gene            complement(9660..11006)
                     /gene="gdhA"
                     /locus_tag="PK105_05855"
     CDS             complement(9660..11006)
                     /gene="gdhA"
                     /locus_tag="PK105_05855"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOU61271.1"
                     /translation="MNSYIKEVLDGLHARYPWESEFLQATEEVLESITPLIEAEPKYR
                     QQKILERIVEPERVVIFRVPWVDDKGEYHVNRGYRVQFNSAIGPYKGGIRFHPKVTLS
                     TLKFLGFEQVFKNSLTGLPMGGGKGGSDFDPNGKSDNEIMRFCQSFITELFRYIGADT
                     DVPAGDIGVGAREVGYMFGQYKRIANEFTGVFTGKGLTYGGSLVRPEATGFGAVYFAE
                     EMLKTMGDTLEGKTVAISGFGNVAWGACMKASQRGAKVVTISGPDGYIYDPDGVGTKE
                     KWNYLNEMLIIDRNKVESYVRKFPNAVFHEGKKPWEQKVDLAMPCAIQNEMSGEDARM
                     LVENGVKVVVEVSNMGCKPEAWKYFIEKKIPFGPGKAANAGGVATSGLEMSQNSMRMS
                     WSAEEVDRRLHEIMTNIHKSCIETAAKYGFEGNYVVGANIAGFKKVADAMIAQGLV"
BASE COUNT         2941 a         2614 c         2486 g         2983 t
ORIGIN      
        1 cgagaacttg tttgactcgc tgactatcga ttatctcgcc ctcgtcgaat gctagaataa
       61 tcctgacttc gagcggatgc agtatccggg ccgcactttg atcaatcatc ttttcctgcc
      121 ttgttgttag agtgaatatt gccggaaatg ctcttgctgg tcaattgcca gtctctatcg
      181 ctcgcttcaa attgcttgat tcggtagatt tgaaaatagc acccttgccg ctccgaccat
      241 caaagcgcat atcaagagag gtatttaact ccgtaagaac acaatgcctt gttcctaccc
      301 tgggcttcaa tgaatagagc cattccagat cgatatgatc atccgctgaa ccttgattta
      361 tcttcatata ttttatacgc atggttgtaa gattatgaaa aaaatgtgac cccagtgagc
      421 tgtccactac aaatcctggc agatccattt cgactataac ttttgcatga gaaatctgat
      481 caaaagatac aggcactcca agccatctat ccctcgtacc ccatcttccc ggcccaatca
      541 acaaataatc gaaattctcc ttccgtgcga ggacgtcgag ttcttctatc tccgccgcga
      601 tttccagagt ctcatttctc tgaaatttgc gtggatcaac ccagactatg ttatgaatgc
      661 tgaggtctct gccattaccc atgcagtttc gagaaagcag aaaacatgca tccttctgaa
      721 tagaactcaa atcgatttca atttgatctt caagatgaat gagcggcttc agctgtagaa
      781 gatacagagt tggttcctgc ttgtcgtcgt caaaattgag tgcatattcg atctccacgg
      841 gtgtacccat agaacgcttc ccgatgtcga gcgctatgtc tattgctttt gcaaatggat
      901 atgcttcatg tttcagtata ttggccaaat ctattatgcg atgtccatcg gtcgaaactc
      961 ccggcacaaa gcgctgatcc agtgaatccc aggtagaagc gaacatggag aaatatctat
     1021 cactctcagc cgcatctatc ggcaattcga atagactagc catctctcct ttaagaagat
     1081 tcggatcttt gtctctaagc gaaagaacat gaaaatagcg ctgtgtattc tccgatatat
     1141 actctagcgg tcctatatcg agctttggat atctggggca gaaacgaaat gccgttcctc
     1201 ctgtaactag atatgttccc aacccaagag ccgcgataca gagtccgtcg tcaggcttta
     1261 cataagagat cggataataa ttgtatgatt gagcaactcc agatatatgt ggataaaaat
     1321 aatctccatg tcggttgccc gccagttctt gtataattac cgccattcgc tcttcttcga
     1381 gattatagtt agtagcctcg aaataggcgc gagcttcctt ggaaaaaagg ctcgcgtaga
     1441 tcaactttac cgctgttgat agctgcttaa agcgtacttc gctgtcggga tgattgtttg
     1501 gtaatatata tgtatcgtat acaccagaaa atggcaccat gagcatgtct tcgaaaagac
     1561 cgctcgaacg cacagccagt ggcctcttgc taagcgatag caactgcctc agattattct
     1621 caagtttctg agaaaggggc ttttgaacaa agaccgagcg gagatcctga acctccttgt
     1681 cgtagtaagc ccaagaccat aggccattcg attcaagaaa gtgctcgaat tcatcgatac
     1741 ctataaacac ggtattcggc attgagatct ctattcctgg cacatactgc ggaaaatcga
     1801 aattctctaa gagcgaacga atgaatagaa gtcccctgcc cttgccgccc acagaaccgt
     1861 ccgctatttt acataaatac ctggagtgct tgaccatctc aggatcgagt tcaggcacta
     1921 tccctctgga atgaaccgat cgaatctggt cgatcatgtt cacaatgaac tgccgtaatt
     1981 cttccggtga accgaaatca gtgattctgt atggcttcat gatctttgct acatttattt
     2041 ctccgcgtgc cataagccaa gctgagaaat gattatgtcc cgcatggtaa agcaggcttt
     2101 ctatgggaat ttcattcaat ttctggatga attcgctcat attacctgct tgtccgatct
     2161 cggctccatc gggcatgcga aagacgaaat tcccaaagcc tagatttgcc tcaaagaaag
     2221 cggcaagttc catctccaag gattccgaat ttttgtcgat aaaactggca ccaaaagcat
     2281 aagctttttc gcgcacattt gtttcactag attgcataag cattggcagg tccggcaatt
     2341 cctgcttcac aaatttaaga aaatcgaaac ctgcattatc atcgaattca ccgtttcttc
     2401 ggaagcgaat atcggtgatc accgtaagaa gataaggctt atagctttca aatacctcaa
     2461 gcgcgtcttc ataggtatta gcaagaagca ccttcggtct ggctcttata ctcatcagtt
     2521 tattagtctc cgcaatccgc tctccttcga taagggcttg agtctgttta agtacaactc
     2581 tatataagac aggtaaatag cgagagtaat atctgaccga atcttctata aggagaatta
     2641 ctcgtacttg tccagcgtga gtgtcagccc tggcatttcg ccaatcttcg atatatttga
     2701 tcattcccac aaagagcttg gaataaccat tccagacaaa gattctattg aagactgcaa
     2761 gttcggggcg gctcatatcc agcattgcta aactggaatt gtccgttacc atgagaagaa
     2821 tgggaatatc tggccaagta ctcttcatcg agactgcaag tcttagtggc atatcaaatt
     2881 ccgtgcttgc cattacgact accaaatcga acctgccatc tctaaaaaga tccatagcgg
     2941 attcttcggt ataagcacag gtgattcttg gaaccgcatt cagattgagt ttgaaatatt
     3001 ctccatatat ttgctctgta agcactccat cagattcaac aacaaaagaa tcatagaggc
     3061 tccctaccaa tagaatttct cgtacgcggt cgcgcattag atcatgatag atattgcgct
     3121 cacgccgctg actgtcatag gtcgaaacga gttcattgaa atacatggct caattttatt
     3181 catattttgc attaaaatga agattagcaa accttgaccc tgtttctatg catagctatg
     3241 attgccgaaa cccctcagag gtttttcgat agatagggat tgaatataag gagatcgccg
     3301 tgttcaagaa tataaaacta gcagcccgca atcctgggca gaagactacg gtcatcgata
     3361 taagcggcgt aaaagtcggt gatgaccttg ttgtcattgc tggaccatgc tcggtggaaa
     3421 cagagaagca gaccattgaa accgctattg cggtaaaaaa tgctggagca aagatgctgc
     3481 gcggaggagc tttcaagcct cgaacttctc catatgcctt ccagggtctt ggattaaaag
     3541 gccttaagat cctcgataaa gcgcgccgtg agactggact gcccatagtc actgaggttg
     3601 tcgacacgcg cgatgtctct tgggttggag agtatgctga tgtactacaa attggtgcac
     3661 gaaatatgca gaatttttcg cttcttcggg aggtaggcaa gtctggaaaa ccaatcctgc
     3721 ttaagcgtgg catgtattcc actttggaag aatggctcaa ttgcgctgaa tacatactcg
     3781 cagaaggcaa tcccaatgtg attctatgtg agcggggaat tcggactttt gaggcttata
     3841 cgcgaaatac actcgattta tctatgattc cggctgttca ccgagaaagt catttgccaa
     3901 tcttcgtcga tccctcccat ggaacgggca ttctatccct tattgagccg atgagtctgg
     3961 cggcggtagc ggcaggagta gatgggctgg aaattgaggt ccatatagat cctaaatccg
     4021 cgctaagcga taaagatcag caactcacca ctgtaatgtt tgagtctctc atgaaaaagc
     4081 ttcgagggct caagcacttc ttggatgaat cgaatgttgc gtctgatgag tcgtctgatg
     4141 agtatgcttc cgaagagtgt atttcatctt cacatgatag gacaaaagcg tcgaattctg
     4201 ttggcacatt gaccgatgcc ttctctgaag ccgtagatta ggaaaaacct aatgcaaaga
     4261 aatcaaagca taatacgatt tggaaagacc tctgagttgc tctcgctaat tcctatagaa
     4321 aaagccatct ttgttacaag cgaggctctt tatggacagg cattagaaat gctgtctagt
     4381 tcaagtctag atgcaaaaga agggctctgc tggatcaaag tccccgatgg cgaagcctgc
     4441 aaaaattttt cggttttgga gactgtgtgt cgccaaatga tagatttgga tgctaatcca
     4501 gatacagtgc ttgtggccat tgggggtggt tctctaagcg atctggtggg gcttacggct
     4561 catctatgga tgcgaggaat tgatatcgcg cttgttccga ccaccttgct tgcaatgatc
     4621 gatgcatcta tcggagggaa aaatgccatc gatataggtc ctacaaagaa tcttattggc
     4681 agctttcata ttcctagact gattctttgt gatgtcggct ggctggaaac cctgccttca
     4741 agagacttgt tatcgggcat ggcagaggct attaagcatg ctgtcatcga tagtgaagag
     4801 catttccgct ttctagaagg aattgcgaat tcggaagaat cgctcaccga aataaatgcc
     4861 gagctttggg aggagctcgt tcagcgatct cagcaggtca agatccggta tgtagatgcc
     4921 gatccctatg ataactatct taggcatgct ttgaattatg gccatacttt tgggcacgca
     4981 attgagctta ttacagagat tcctcatgga tttgcggtgg caactgggat tggtatggca
     5041 aacgatcttg cggtatctcg cggcgctctg gatccggata tcgctgcccg cataaattcg
     5101 cttttgcata gtattggtct gccggcgaat gttgcggaag tttctgcgat tgctggctgc
     5161 aatattcgtc cagcggcaat atacgatctt atacgggctg acaaaaaaag gcggggagag
     5221 agcatcgatc tcgcactgct acgaaatatt ggcgatatcc gaatcgagcc tgttgcacta
     5281 caatatatag cggaattctt gggagcttag gcatttctgg agttaataag gggaatcatg
     5341 aacacgatag gcaaaatatt ctgtatttct ctctatggtg agtctcatgg taaagccgtt
     5401 ggagttgtaa tcgatggctg cccagccggt atttcgctaa ccgaagaaga ttttgcagaa
     5461 gatctgaatc gaaggaaggc aggggcggaa ggaacaacga caagaacgga atcggataag
     5521 ccgattcttc tatcgggagt ctttgacggt catacaaatg gcgcgcctat tcatatcgca
     5581 ttccgaaatg agaatatccg atcctcagac tatgagcaat ttatagatat tccgcgacca
     5641 ggtcatgctg atttcagcgc aatgatgaaa ttccagggat ggcaagatcc aagaggatcc
     5701 gggcatttct cagggcgtgt atccattggc cttgtcgccg cagccgttgt cgcaaagaaa
     5761 atcctcaaca gcttttccga tattgttttt cggacaaggt tgctcgaagc cggtgggtct
     5821 agagatatag acaaagcggt ttatcaagca aaagaatctg gagattcgat aggcgctctt
     5881 gtggagatta gagtatccgg agtaccggcc ggatggggag agcctttttt cgatactgcc
     5941 gagagcataa tcagccatta tatttttgca gtgccggccg tacgtggcgt ggaatttggc
     6001 gatggatttg ctgctgcaaa aatgcgcggc tcccagcaca atgatccatt catagacttg
     6061 catggaagaa cagcacgcaa tggagcgggc ggtatcaatg gcggtattac caatggaaat
     6121 gatattttgt taagagttgc ggtaaaacca gcatcatcca ttgcctctac gcagaacacg
     6181 cttgattttt ctcgcgatga gatggttaat cttgaaattc atggacggca tgatgcctgc
     6241 atcgctcttc gaggcgcggt agtcttggaa gcagccacgg cctgtgctct ggccgatctc
     6301 gctcttgtgg cacgcgcaga agctccgttt atatccaata tcccatggag gaaatcatga
     6361 atctcgatga tattcgaagt gacatcgatc gcatagatgc caaaatactc tcgctcctaa
     6421 atgagcgaat ggaaaaatca cttattgcga ggcacttcaa ggcagtagcc ctggatccgg
     6481 tgcgcgagca gattgtcctt gaaaaggttc gccgctcgag tcaatgcctc ttggatccgc
     6541 atttctcggt tcatctctat gagcagataa tatcggaatg ccgacacctc caggatgccg
     6601 gccttaagac cgtagcgttt caaggcgaac acggagcgta ttcggaagtt gccctgcgat
     6661 tgcttatgcc aacagcggcg accattccgt gttgtgaatt ctcggatgtc ttcgatggtg
     6721 tcgagaaggg tatctacgac tatggcatcg ttcctgttga gaatacgctt ggaggcattg
     6781 tagggccggt aaattcgatt cttatatata cacgcttaaa aattgttgca gctatcgact
     6841 tgccaattcg acactgtctg ctcacaattc ccggagccga tcatcgtgaa ttgagaatag
     6901 ctcgatctca tacgcaagct ttaacacaat gccgcaattt tttgctaagg aaccatctgg
     6961 acccagagcc tttttttgat acggcgggcg cggcaaaagc ccttgcggag gaaagaccca
     7021 agggtgtatc cgttgtggca agcaaatttg cagcagatct ctatggcctg gaaataatca
     7081 aagaggatat tcaagattct ccgcataatc gaacgcgctt ctttattatc gcgacagagg
     7141 aaaacggggt tgagggcgac aagtgttccg ctgtttttac ggctggaaac aaggccggtg
     7201 ctctattctc agtgctcgag gtattcgctg aagaaaatat aaacctaacg cgaatagaat
     7261 cagtgcccga tgtgccggaa aaatatgcga tattcataga ctttgagggt tcactctctt
     7321 cagctccagt gcaacgggcg ctggaaaaag ttccggagat tgccgaagat tttaaaatcc
     7381 tgggatgcta tcatgaaatg agggtagaat catgagaatc gcaatactcg gcgcaggccg
     7441 aatgggcgcg tggctcacgg aggagctctg ttggaataat gaagtcatgg ttcatgacag
     7501 cgatctcttg aagatgaagt attttatcaa ggtacaccga gcgcttgcca tagaggagtt
     7561 tgccgatttc aagcccgagc tctttataaa ctgtgtacct cttggcttga cacttgattc
     7621 ctttgacgca gttcttccat atttgccttc tcaatgcatc attagcgata tagcaagcgt
     7681 aaaaacaggc tttcgagaat actacgaaaa ttgcagacat ccatttgttt cgagccaccc
     7741 catgttcgga ccaacccttg caaatatcag agaccttcat gaagaaagtg caatcattat
     7801 ttcggaatca agcaaggagg gcgcggattt ctttcgtcga ttctattcaa gcctaagaat
     7861 ttgcattttt gaatatagct ttgaagaaca tgacaagact gtcgcctatt cgcttgctac
     7921 tccttttgct tctacaatgg tatttgcagc ctgcatgaaa aagcaagatg cgcctggaac
     7981 gaactttaaa cgacacctgg caattgcaaa gggcttgcta tcggaagatg atcgtctttt
     8041 gacagagatt atgttcaatc cctataccat acgccaactg gagcttataa actctaaatt
     8101 agcttatctg acccatataa ttcgacagcg agattatgat gaaatgaaga aattccttga
     8161 taagctgcgc gctaatatag aacaatagac aagaatctct gaatcgccgc taacccttca
     8221 catggatcga atcgatattt attggactcc taagctcttt tggcacttca gccttaaact
     8281 agcgaagtga taatcaagaa accactatag aagaaagatc atcaaaaaag cccggccagg
     8341 acttggatac acactctgcc ccttcgatgg atacttcgcc acttgctaca agagctgcga
     8401 tggaggctgc catagcaata cgatgatctc catatgattc aacagtagct ccatgaatag
     8461 atccacctac tatgtacatc gcatcgttct gcaatgttac atctacacct agcttccgaa
     8521 atgtcttgca caatgctact gagcgattgc tttctttgga taaaagccgc tttgtccctc
     8581 tgatacagga cctgccctga atggcagctg ccatggctac gaggggagga aaaaggtccg
     8641 gacaatttgt tgcatcgaaa tcaaagggag caagggcgct tgctcttaca atacaatccg
     8701 agcctttgaa ttgcacttga gcccccgctt cagttagaac atccacaatg gcgcgatcag
     8761 gctgtgttga gtcttgatgc aaaccatgga tctcaaggcc ttcttcactt cctgctattg
     8821 cagcgcccac agccaggaaa gcaccggcac tccaatcgcc ctcaataatc aaatcagtag
     8881 ccttatagac ctgattcccc ggtatccaaa agccggcacc attcgcattt ctttttattt
     8941 cgaccccaaa tctggcacat atttcaatgg tgagatcgag atatcccgag cttacaagat
     9001 tatctacaat tagctctgaa tcaccatcca aaagaggcaa agccataaga aggccggtaa
     9061 tgaactgaga gctccctaga gcatcgatcc tgctcttcaa gggatgaaga ggaccctgga
     9121 ttcgtatagg ctgaactccg cctctcgata tacaagagat gccaaggctt tttagagaat
     9181 cctcgaccat attcataggt cgactagaaa gagatgcttc tcccctaagc tctacctctc
     9241 tgtccaacag agcaacaatg ggagagaaca ttctcatgca aagccctgat tctccacatg
     9301 gcaggactag gggtaagcat ggttgaaatc cattgttttt caggcctgga tagcatactt
     9361 tttgcgatgt agtggccaat ttgcctttgg catctttatg aaacaggggc gatccttcga
     9421 tttcgagaat ccctttttct tccttaacct ttgctcccag ctcttttgca atgataattg
     9481 cagcgcgcgc atcgctgcat acaggacttg cctctatatg gctcattcct cgtgctagaa
     9541 tggcacaagc gatcgcgcgc tgaagggcac tcttcgatgc cggggcttgt atgctaccgc
     9601 aaaccttccc cggtttcgcg accctggttc ccatagaatc tatacaaaaa cctctacaac
     9661 tacacgagtc cttgcgcaat catagcatca gcaactttct tgaatccggc aatgtttgcg
     9721 cctactacat agttgccctc gaagccatat tttgcagcgg tttcaataca actcttatgg
     9781 atgtttgtca ttatttcgtg caatcgtcgg tcaacttcct cggcgctcca ggacatacgc
     9841 atcgaattct ggctcatttc aagcccgctt gtcgctacac cgcccgcatt tgccgctttg
     9901 ccggggccaa aaggaatctt cttctctatg aagtacttcc aagcttctgg cttacaaccc
     9961 atattcgaga cttcgacaac gaccttgaca ccattttcga ctagcattct tgcatcttcg
    10021 ccactcattt cattctgaat agcacaaggc atcgctaggt ctaccttctg ctcccacggt
    10081 ttcttgccct cgtggaacac ggcgttgggg aattttctga catagctctc aaccttgttg
    10141 cggtctatta tgagcatctc gttgagatag ttccactttt cttttgtccc tactccatcc
    10201 ggatcataga tatagccatc cggcccggaa atggtaacca ccttagcgcc gcgctggcta
    10261 gctttcatgc atgcgcccca tgcaacattg ccaaacccag atatggccac cgtcttgcct
    10321 tccaatgtgt cacccatggt cttgagcatt tcttcagcga aatataccgc accgaagcca
    10381 gtggcttccg gcctgacaag cgaacctcca taagtcaagc ccttgcctgt aaagacaccc
    10441 gtgaactcat tcgcgattct cttatattgg ccaaacatgt agccaacctc acgggcacct
    10501 actccgatgt cgcccgcagg cacatccgta tctgcgccta tatagcggaa tagttcggta
    10561 atgaagctct ggcagaagcg catgatctca ttgtcgctct tgccattcgg atcgaaatcc
    10621 gaaccgccct tgcctcctcc cataggaaga ccggtgagcg aattcttgaa tacctgctca
    10681 aatccaagga acttgagagt ggagagagtg actttggggt ggaatctaat accaccctta
    10741 taaggcccaa tggcggagtt gaactgaacg cgatagcctc ggttgacatg atattcgccc
    10801 ttgtcatcta cccatggcac acggaatatc actactcgct cgggttcgac gatccgctca
    10861 agaatctttt gctggcgata ttttggctct gcttcgatga ggggagtaat cgattcgagg
    10921 acttcctcag tagcttgcaa gaattcgctc tcccatggat atcgagcgtg aagcccatcc
    10981 agcacttctt ttatgtagga attcatttct ccctcctttt aggg
//
LOCUS       DAOOWY010000062        10841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_6053_length_10841_cov_16.473855, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000062 DAOOWY010000000
VERSION     DAOOWY010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10841
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6053_length_10841_cov_16.473855"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..320
                     /locus_tag="PK105_05860"
     tRNA            248..320
                     /locus_tag="PK105_05860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:281..283,aa:Ala,seq:cgc)
     gene            433..1683
                     /locus_tag="PK105_05865"
     CDS             433..1683
                     /locus_tag="PK105_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOU61272.1"
                     /translation="MARKPVSVFKRPTTKKGQFRYYIKVWDENKGRYSSPRSAISIAR
                     ELGLDEKRYPPTSRTGALLIGQELLKRGGILSKKSDPLFADYCASIWDWDTSPYIQGR
                     IARGLRIGKEHAKHCASYIEHYVRPAFPSLKLSALRPYMLESFVLSLKKNSGLRNRSI
                     NAIIDAIRTPLKEAVRLGLIAVDPSASLQKLSNDRRVKGIPTEEELSAILALDLDLRI
                     RCAILLGAVCGLRLGEIQALKLEHIKDNTLHIVASWGKIDGLKETKTGRSRIVPLPAI
                     IKDALLELSQKNPHGPDGFLIYGFRPDAPLDCRAIERGFDHALVKLILGEKYANASRE
                     EKAAALRTWRERNITFHSLRHFANAALRGSVPDETLRKLTGHSTEAMTNHYDHTTKSD
                     LEALAEAQEKRILPLIKRHRVTTT"
     gene            1804..2277
                     /locus_tag="PK105_05870"
     CDS             1804..2277
                     /locus_tag="PK105_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61273.1"
                     /translation="MLVVVSFQPPPMIQIAIKPGDEGIPFGAIGTDRELGSKVFLKNG
                     KPVTRRVIQYWRQGKKRIPEWAQRQLSNIYWRKVILNNMLLQYGQNGPPPSPEIFPYL
                     IPGMEGMLMNPMYWKRVRRDGRPIYTPNNATVPAAAQLALSNFPQRILSMQPKTN"
     gene            2449..2643
                     /locus_tag="PK105_05875"
     CDS             2449..2643
                     /locus_tag="PK105_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HOU61274.1"
                     /translation="MGFNKKFLSRQEAADALGISLVTLARRLRDQSIPYIKIGARVLI
                     PAAFIDQLLEKALSDREVRL"
     gene            2640..3077
                     /locus_tag="PK105_05880"
     CDS             2640..3077
                     /locus_tag="PK105_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61275.1"
                     /translation="MRCAGKKKPAASRAGLRNWATSKIYNVNLTEEASSVKLPAWLVE
                     KLGRIAVRRDIWQFEQEEAILLAESIRLFPDIAAILPEDFRNRASKGIPRELTPYERA
                     AVLYVADCWRKEVCDIWEGCTLEEKIRARLEALMELLREGGAV"
     gene            3074..4132
                     /locus_tag="PK105_05885"
     CDS             3074..4132
                     /locus_tag="PK105_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU61276.1"
                     /translation="MNSFDDFEALVERLPQEEVQRRERLAKVQIMNGAELMLKRFPEP
                     QYLWDSVLPDAGLAVMAASKAAGKTLLLLQLANAISRGREFLGLPTRLTKVLYLQLEL
                     SERRTQQRLLKMGIVANEYLHFAHRWLCGEEGLLTLADAIKEHRFGLVIVDVMQLLWP
                     MDADTNSYQDVYSVLGPLRQLANDLGVMILLVTHRRKAETADYLDGVIGSVGITANAD
                     VVFSLIRNRGEDEAVLHIDGNDIESRKIALRFSADPLGFTLSDAQPGEIGLTAERREV
                     LEAIRKLGGCARTHDIALSIGKKDDTTSHLLDKLADLGLLVRMGYGQWAIRNSIQSDQ
                     SIQTDPITLSRLNTLNGT"
     gene            complement(4611..5265)
                     /locus_tag="PK105_05890"
                     /pseudo
     CDS             complement(4611..5265)
                     /locus_tag="PK105_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5363..6580)
                     /locus_tag="PK105_05895"
     CDS             complement(5363..6580)
                     /locus_tag="PK105_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU61277.1"
                     /translation="MIQRELYLKKIRPFMNTPVVKVFTGIRRCGKSVLLQLVQDELRQ
                     QGVGPECILALNFESAADSRIRSTASVLEAVHEQQQRCGPGRLYLFFDEIQELSGWET
                     LVNALLVDTDADIYITGSNARMLSSELATYLAGRYVEIRVYPFSFAEVVTLLSQQGGR
                     EIRPEDAFKLYLRRGGFPFLYNYPFTDADANQYLNDIFDSTILKDIAQRNAIRDIAQL
                     RRLVLYFIANIGNTFSATSLVKYLKNQRRSISTETIYNYIEYCRAACLLHLAQRQDLA
                     GKALLTTQEKIYLVDHGIREAIYGNNQRDINQVLENIVYMELLRRGYDVTVGKTKNAE
                     VDFRASRGQDVVYVQVCYLLADESTVEREFSALESITDNYPKYVVSMDEINRSRNGII
                     HLNIRDFLLGDAV"
     gene            6756..7055
                     /locus_tag="PK105_05900"
     CDS             6756..7055
                     /locus_tag="PK105_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU61278.1"
                     /translation="MRKRYDKEFKAKVAVEALKGDKTLQELAITYSVHPNMIALWKRQ
                     LLEHASMLFEKEGKDKASEEAERQKGELFKQIGQLQVENEFLKKYKQLYGTDPKW"
     gene            complement(7840..10575)
                     /locus_tag="PK105_05905"
     CDS             complement(7840..10575)
                     /locus_tag="PK105_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOU61279.1"
                     /translation="MIQRHVFQRSRSQPGTQFLASRLQGAVRYDRIAGYFDSSLFELA
                     GEAFEQVRGSIRIVCNSDIRQQDIEAASAAAREQAQRLSFFKHDPEELAQSGTERLER
                     LVRLLRGEGRARLEVRVLPDDVFGLIHGKAGVITYADGRRTSFLGSANETLAAWTLNY
                     ELMWEDDSPEACDWVQAEFDRLWHHPLAMPLTQAIAKEVERLAHRTEVAIDEWREKPE
                     AASVAIESPVYREQFGLWPHQQYFVSLAWRAHQAHGARFILADQVGLGKTVQLGMVAQ
                     LIALTSDKPVLAILPKTLMEQWQVELWDLLQVPSARWNGSSWVDETGFVYPAATSEPL
                     LACPRKIGLVSQGLVVHSPESLHCLLERDWSCVVVDEAHRARRRKLPAPDEYGPAIRN
                     PDTECNRLYAFLYKLASRTESMLFATATPVQLHPIEAWDLLFLLSQNNPHVLGEIGSI
                     WLNPEQAIPAMLGIREPPQEASEVWPWVKNPFPPSWESPHAKALRMDTQMTEHDAVCK
                     ASFHELRPASRTRAVSLCTVFFDQHLPFVRSIVRRTRSYLESTINPDTHEPYLTPIGV
                     ELFGEDDPIPLTGYMADAYEEAEHFCQALSRRIRAAGFLKTLLLRRIGSSIEAGRRTV
                     DAMLAKTLGQVAEDDEDQEGVQDADAQADTLSELYPFTHEEQEILVRCAKLLDTATEE
                     DPKWDVVLHYLKDEGWGQEGCVVFSQYYDTASWVAKKIATAFPGQPIGLYAGSGKSML
                     LQDGRELRKEREELKQMVGRHELTILIGTDAASEGLNLQALGTLINIDLPWNPTKLEQ
                     RKGRIQRIGQKRASVKILNLRYKDSVEDRVHQVLAGRLQEIYRLFGQIPDVLEDVWID
                     VALGEVEKARQELDRLPVRNPFDERYGTLQRVTDWETCSQVLNRHEKLELLKKGW"
     gene            complement(10572..>10841)
                     /locus_tag="PK105_05910"
     CDS             complement(10572..>10841)
                     /locus_tag="PK105_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU61280.1"
                     /translation="TQQVTQQVTQQVTQQVTSEETQRRFAAVLEFCEIPRTRKEIQDM
                     LGLKDRENFRVTILTPLLNSGQLELLYPDRPRSSHQKYVRKRLQP"
BASE COUNT         2670 a         2690 c         2833 g         2648 t
ORIGIN      
        1 agcccggtag agaaaagatt gcaaaatggc ccaataggca agagaatgct gcttgctgcc
       61 tagagggacg gcgcaaatag aaaaggctat aaagggcaca gaagcgactg gcccttgatt
      121 atggcctttg ctctttgcta tattttatat tcgcctttgg caaaagaaaa cggaaagcaa
      181 gtggctgccc gatggatata tgcagcagcc aaccaaattt ccgttttact tcttataagg
      241 cctttgcggg gaattagctc agctggtaga gcgataggtt cgcaatctat aggtcagggg
      301 ttcgaatccc ctattctcca tcagataagt cattgataga ttatgactta tagaaagaca
      361 gaaaaaccgg atttcgattc aattccttta ctgttaccca tttgttaccc gaattggtaa
      421 tggagggatg gaatggctcg gaagccggtt tctgttttta agcgacctac caccaaaaaa
      481 ggacagtttc gttactacat caaggtctgg gatgagaaca agggcaggta ttcttctcct
      541 cgttctgcaa tctccattgc ccgagaactt ggacttgatg agaagagata tccacctacc
      601 tctagaactg gggctcttct cataggtcaa gagcttctca agcgtggcgg catactatcc
      661 aagaaaagcg atccgctctt tgctgactat tgcgcctcaa tctgggactg ggacacatcg
      721 ccctacattc aaggccgcat tgctcgagga cttcgcattg gtaaagaaca cgccaaacac
      781 tgtgcgtcct atattgagca ttatgtacgg cctgcgtttc cttcgctcaa gctttctgca
      841 cttcgtccct acatgctgga atcattcgtt ctatcgctca agaaaaattc aggacttcga
      901 aaccgaagca tcaacgcgat catcgatgct ataaggaccc cactcaagga agcagtccgt
      961 ttggggctca tcgcagtaga tccctcagcc tcactgcaaa agctcagcaa tgatagaaga
     1021 gtaaagggga ttcccactga ggaagaactc tcagcaattc tggcgctcga tcttgatttg
     1081 cgtatacgat gtgccatcct gttgggagct gtctgcgggt tgcgattagg agagattcaa
     1141 gccctcaagc ttgaacatat aaaggacaat accctccata ttgttgcttc ctgggggaaa
     1201 atagatggcc ttaaagaaac aaaaaccggc agaagcagga ttgtgcccct ccctgcgata
     1261 atcaaagatg ctcttctcga actctctcaa aagaaccctc atggtccgga tggctttctc
     1321 atctatggtt tccgtcccga cgctcctctc gattgccgag cgattgagcg aggtttcgat
     1381 catgctctgg taaagctgat tctgggcgaa aagtatgcaa atgcgagcag ggaagaaaag
     1441 gctgctgcgc ttcgtacatg gagggagcga aatatcacgt ttcattccct caggcacttt
     1501 gcaaatgcag cattgcgtgg gtcagtcccc gatgagacat tgcggaagct caccggacac
     1561 tccacagaag ctatgaccaa tcactatgac catacaacaa aatcggatct tgaagccctt
     1621 gcggaagctc aggagaagcg tattctacct ttaataaaac gacaccgagt tactaccaca
     1681 taacatcttg actgatactg atacaaaatt gtatggagta tgcgaattgt aactttctaa
     1741 ggggcgtgct tgttagtata attttgaaag ccgctcagaa tatgtcatag tatggaggtt
     1801 taaatgctag tagtggtatc atttcaaccc ccgcctatga tacagatcgc catcaaacct
     1861 ggcgatgagg ggataccatt cggcgcaata ggtactgata gggaattagg tagcaaagta
     1921 ttcttaaaaa atggcaaacc tgtaactcgt cgtgttattc agtactggcg ccaagggaag
     1981 aaaaggatac ctgaatgggc acagcgtcag ctcagcaata tctactggcg taaagttatt
     2041 cttaataata tgcttttaca atatggccaa aatggtcctc ctcccagtcc agagattttc
     2101 ccttatttaa ttccgggaat ggaaggaatg ctgatgaacc caatgtattg gaaaagggta
     2161 cgtagagatg ggagaccaat atacacccct aacaatgcaa cagtaccagc tgcagcacaa
     2221 cttgctctca gcaattttcc acaacgtatt ttgagcatgc agcctaagac aaattaaaca
     2281 ctgatttggg gaaatcaggc tattgaggga aatagaaatt gccgtaaggc gagcgtcccc
     2341 caatagtgtg cctctcgcct cgtagagtgt cgaaggcact agttgaccgt tttgtaggcg
     2401 actccttatc tcgcccctca taaagagggg tgatattagg agtcccacat gggattcaac
     2461 aaaaaatttc tctctcgtca agaggcagcc gatgcactcg gtattagcct cgtcaccttg
     2521 gcgcggcgtc ttcgcgacca atcaatcccg tacatcaaga taggcgccag agtcctcatt
     2581 ccagcagctt tcatcgatca gctcctcgag aaagctctct ctgaccggga ggtgcgtcta
     2641 tgaggtgcgc gggcaagaaa aagcccgcgg ctagcagggc gggtcttcga aactgggcaa
     2701 cgagcaagat ttacaatgta aatttaacag aagaagcatc ttctgtcaag ctacctgcgt
     2761 ggcttgtcga gaagctaggg cgtatcgctg tgcggcgtga catctggcaa ttcgagcagg
     2821 aagaagcgat tctgcttgct gaatcaatca gactatttcc cgacatcgcc gcgatcctac
     2881 cggaagattt tagaaatcgc gcatcgaaag gcattccgcg cgagctcaca ccatacgaac
     2941 gggctgcagt tctctatgtc gctgattgct ggcgcaaaga agtatgtgac atctgggagg
     3001 gctgcacgct cgaagagaag atcagagcac gactggaagc actcatggag cttctccggg
     3061 aaggaggcgc cgtatgaaca gctttgacga cttcgaagcc ttggtcgagc ggctgccgca
     3121 ggaagaagtg cagcggcgtg agcggctggc aaaagttcag atcatgaatg gggcggaatt
     3181 gatgctgaaa cgctttcccg aaccgcagta cctgtgggat tcagttctcc ctgatgcggg
     3241 ccttgccgtg atggcagcat cgaaggccgc aggcaagacg ctgctgctct tacaactggc
     3301 caatgccatt tcacgaggaa gggaattctt agggcttcct acacggctaa caaaagtgct
     3361 gtatctgcag ctggagcttt ccgaacgccg cacgcagcaa cggcttttaa aaatgggaat
     3421 tgtcgcaaac gaatacttgc acttcgcgca tcgctggcta tgcggcgaag aagggctgct
     3481 cacgcttgcc gatgcgatca aagaacatcg cttcgggcta gtgattgtcg atgtcatgca
     3541 gctgctctgg ccgatggatg ctgataccaa cagctatcag gatgtgtatt cagtcctagg
     3601 cccgcttcgg cagctggcga acgatctggg cgtgatgatc cttttggtga cgcatcgaag
     3661 gaaagctgag acggccgatt atctcgatgg cgtcataggc agtgtgggca tcacggcgaa
     3721 tgccgatgta gtcttttcgc tgatccgaaa ccgcggcgaa gacgaagctg tactgcatat
     3781 cgacggcaac gacatcgaaa gccggaagat cgcgctgcgc ttttctgctg atcctttggg
     3841 ctttacattg agcgatgcgc agcctggtga aatcgggctg accgctgaac gccgtgaggt
     3901 gctggaagcg atccgaaaac tgggtggctg cgctcgaacg cacgacattg cactttcaat
     3961 cggcaagaaa gacgacacga cttctcacct gctcgacaaa ctggctgatc tgggcttact
     4021 ggtgcgcatg ggctacggcc aatgggcgat tagaaacagc attcaaagtg atcaaagtat
     4081 tcaaaccgat cctatcactt tgagcagatt gaacactttg aatggtactt agaagcattt
     4141 gttttgtcgg caccatgttt ttgcttcgct ttttgagcat tttaagaaat atttttctta
     4201 aatagcaaaa acgaaatgta tagcattaac aaataatata gtgccccctt ccgagttcat
     4261 tataatgagt atgaacgaag agggggacac tatacttctt ccattctcgt tcatcaaatt
     4321 ttttcttaga attggtctgg ctcgagaagc tcagcatagt ctgtctaatt atttgataga
     4381 aaagcgcatt agaattggga tgaatactgc gaatatattt acaggagatc aagcgattat
     4441 ttttaagata aaaagcccta atagctttgc ttgagtcttt ttgagactgt aaatctacaa
     4501 taagttgcag ctatctatta agcatatcat ttgtattttt ctagtaaagc caaaaatttt
     4561 ttctcatttt ttctcatttt ttattcttag gggctctagt agataagact ttaaaccgaa
     4621 caccattccc ttaggacctt gagttgattt ttgggtgttc catagttgaa acggaactca
     4681 cattctttca aaaacaaggg aaagtatttc ttatcgatgc cattgtactt tcttaagact
     4741 cgtttggctt gattccagaa gttttctatg ccgttgatat gatgcttctc atcagcaaat
     4801 ccctctgtat gattgattct gtaatgatga aactcactca catccaatgc atcgtagcta
     4861 tgataggcgt ctgtataaac cacgctatct ggcttgatgt taagggctat ttcgttcatg
     4921 agcgtctttg tcttggtatt ctctaccacc acggtataca ccttgccatg gcgcttgagc
     4981 atgccaaaga cgaccacttt cccggcagcg cctcgaccgc gtttaccttt gcggactcca
     5041 ccgaaatagc gttcatcgat ttcaatgtcc ccttgaaata tttcatgggc ttcttttgct
     5101 agattagaag caataacttg acgaattttt cgatagaaaa gagctgctga attagggtgt
     5161 attcccagta tatttgctgc agatcttgcc gttacttcca ggacaaagaa ttctaaaagt
     5221 cttgtctgag tattttttcg ttaatctaca atgacttaag cccactttct aagtatatca
     5281 tcgcgcttta tctagtacag ccccaaacaa taaaatgacg aataaggtct aatagcgtcc
     5341 ttcgcggatt cggtgatttt tgtcatacag cgtctcccag gaggaaatcg cggatattga
     5401 ggtggataat gccgtttcgg ctgcggttta tttcgtccat ggacaccaca tatttggggt
     5461 agttatccgt gatactctcg agggccgaga attctcgctc gacggtactt tcgtcggcaa
     5521 gaaggtagca gacctgaaca tagacaacat cttgcccgcg gcttgcacga aagtccacct
     5581 cggcgttttt tgttttcccc acggtgacat cgtaaccacg gcgtagaagc tccatataca
     5641 caatattttc cagaacttga ttgatgtccc gctggttgtt gccgtagatg gcttcgcgga
     5701 tgccgtgatc caccagatat attttctctt gcgtggtcag cagggctttc cccgccaggt
     5761 cctggcgttg cgcaagatgc aaaagacagg cggcgcggca atactcgatg taattgtaaa
     5821 tggtttcggt agatatgctg cggcgctggt ttttcaggta tttcaccagg gatgtggctg
     5881 aaaaggtgtt gccaatattc gcgataaaat acagcactaa gcggcggagc tgggcgatat
     5941 cacgaatggc gttgcgctgc gcgatatctt taagaatcgt tgaatcaaag atatcgttca
     6001 gatactggtt tgcgtcggca tcggtgaaag gatagttgta caggaaggga aaaccgcccc
     6061 tgcggaggta gagtttaaag gcatcttccg gccggatttc cctgccgccc tgctgggaca
     6121 agagcgttac gacttcggcg aacgaaaagg gatacacccg aatttccaca tatcgtccag
     6181 ccagataggt tgccaattca ctggagagca tgcgggcatt ggagccggtg atgtagatgt
     6241 cggcatcggt atccacgagc agcgcattga cgagggtttc ccagcctgag agttcctgga
     6301 tttcgtcaaa aaaaagatag agccgtcccg gaccacaccg ttgttgctgc tcatggaccg
     6361 cttcgagaac ggacgcggta ctgcggatcc ggctatcggc agcggattca aagttcaggg
     6421 cgaggatgca ctccggtccg acaccctgct ggcgaagctc atcctgaaca agctgtaaaa
     6481 gcaccgattt accgcaacgg cggatcccgg taaatacctt gaccaccggg gtattcatga
     6541 agggacggat ctttttcaga tatagttcac gttggatcat gctctaatag taattcaagt
     6601 atatcgaaaa aacaatggta attttattaa ttttttcgat atggcgaaat tttcgtttga
     6661 tagccacagt ataatgagcc gcgaaaactg gacaggaata gagagatcat aaggtggacc
     6721 gctcctcctt gcagaatgga aagaaggagt tagctatgag aaagcggtac gacaaagaat
     6781 tcaaggccaa ggtagccgtg gaagccctca agggcgacaa gacattacag gaattggcaa
     6841 ttacctattc ggttcatccg aatatgatcg cgctctggaa aaggcagctt ctggagcatg
     6901 catcgatgct ctttgaaaaa gaagggaagg ataaggcctc agaagaggcg gagcggcaga
     6961 agggtgagct atttaagcaa ataggccaac ttcaggttga gaacgagttt ctaaaaaaat
     7021 acaaacaact gtacgggaca gatccgaaat ggtagaagcc gatcatcctg agctctcaat
     7081 agcgcaagca atacaggatt ctggggatca gtagatcatc ctactactac gagcctaagg
     7141 aggttcctga agatccagaa ttggccattt tagaagcgat tcttgaggtt ctgaaagaaa
     7201 agccattcta cggttacagg agaatcgcca gggaagttgg ttacatgggc gttacgcgaa
     7261 agcaaattgt caatgccgat ttacatttgc cggttttcgc ctgaactgac cccctgcaag
     7321 tggacacaag agggggtata gaatgaagta ccgtaagatc gagaggaggc ccatgaagta
     7381 tcctctggaa ctcaagatga aggtactcaa ggattatttc gatggcgtag atggcatccg
     7441 cggtctggag cgaacctatg gcattaagca ccagctgatc ctttgttgga tcaagaactg
     7501 tggagaccct cctggattta ttcggccatc aaaagaaaca aaggaagcct gtttacacct
     7561 ccacgaacct ccagcatttc aagatccgcg aaaagagctg gaatatctcc gaattcctcc
     7621 catgttgcaa gtctttctta tttcagatta tacgttaccc tcattccatg ctgcaatgcc
     7681 gaaaaacccc gcttcggtac cgcgaagatc tgcgaagagt ctgctccacc ttcgccatca
     7741 gaacggcccg aaccggatcg ccaccgccag cgcgagaaag gccgaggtga ccgcgaggat
     7801 aatgagttgc ggtatcgcag ggactaggat gatagcaccc taccacccct tctttagcag
     7861 ctccagcttc tcatgccggt tgagcacctg ggagcaagtt tcccaatcgg tgacgcgctg
     7921 cagtgtaccg tagcgttcgt cgaagggatt gcgcaccggc aggcggtcga gctcctggcg
     7981 ggctttttcg acttcgccga gggcgacatc gatccagacg tcttccagca cgtcgggaat
     8041 ctgaccgaag agccggtaga tttcctgcag tcggccagca agcacttggt gcacgcggtc
     8101 ctcaaccgag tccttgtacc gcagattgag tatcttcacc gatgcccgct tctgtcctat
     8161 ccgctggatg cgccccttgc gttgctcaag cttggtaggg ttccatggca ggtcgatgtt
     8221 gatgagtgtt cccagcgcct gcaggttgag gccctcgctc gcggcgtccg tgccgatgag
     8281 gatggtcagc tcgtggcgcc cgaccatctg cttaagctct tcacgctcct tgcgcagctc
     8341 acgtccatcc tgaagcagca tggatttgcc cgagcctgcg tagagcccga tgggctggcc
     8401 cgggaacgcg gtggcgattt tcttggctac ccaggaggcc gtgtcgtagt actgcgagaa
     8461 gaccacgcag ccttcctgtc cccagccctc atccttcagg taatgcagca cgacgtccca
     8521 tttggggtct tcttccgtag ccgtatcgag gagcttggcg caacgcacga ggatttcctg
     8581 ctcttcatgc gtaaagggat agagctcaga cagggtatct gcctgcgcat ccgcatcctg
     8641 tacgccctcc tgatcttcat catcttccgc tacctggcca agcgttttcg caagcatggc
     8701 atcgacggtc cgtctgccag cttcgatcga gctgccgatg cgccgcaata ggagcgtttt
     8761 caaaaagccc gcagctcgaa tgcgccgaga gagggcctgg cagaagtgct cggcttcctc
     8821 gtaggcgtct gccatgtagc cagtaagggg aatggggtca tcttcaccaa agagctctac
     8881 accgatcggc gtaagatagg gctcgtgcgt atcggggttg atggtcgact cgaggtagct
     8941 tctggtgcgg cgtacgatac tgcgcacaaa ggggaggtgt tggtcgaaga acaccgtgca
     9001 caggctcact gcccgagttc gtgaagcggg ccgcaattca tggaacgaag ccttgcagac
     9061 tgcatcatgt tccgtcatct gggtatccat ccgaagcgcc ttcgcatggg ggctttccca
     9121 tgagggcggg aagggattct ttacccatgg ccatacttct gacgcctcct gaggcggttc
     9181 ccgtatcccc agcatggccg ggatcgcctg ttcgggattg agccagatgc tgccaatctc
     9241 gccgagcaca tgcggattat tctggctcag caagaaaagc aggtcccatg cctcgatggg
     9301 atggagctgc accggtgtgg cggtggcaaa cagcatactc tcggtgcggg aggcgagctt
     9361 gtacaggaag gcatagagcc tgttgcattc agtgtcggga ttgcggatgg caggcccgta
     9421 ttcatccggc gcggggagct tgcgccgtcg tgcccggtgg gcttcgtcca caacgacgca
     9481 ggaccagtcg cgctcaagga ggcagtgcag cgattcagga ctgtgcacca ctagcccttg
     9541 agacacaagg ccaattttcc gcgggcatgc gaggagcggt tcgcttgtcg ctgccgggta
     9601 cacaaagcct gtctcatcga cccaggatga gccgttccag cgcgcgctgg gcacctgcaa
     9661 aaggtcccac agttccacct gccactgttc catcagcgtt ttgggcagaa tcgccagcac
     9721 cggcttgtcg gaagtgagcg caatgagctg agcgaccatg ccgagctgca cggttttgcc
     9781 gagcccgacc tggtctgcca ggataaaacg cgctccgtgt gcctgatgcg cgcgccaggc
     9841 aaggctcaca aagtactgct ggtgcggcca gagtccaaat tgctctcgat acaccggcga
     9901 ttcaatcgcg acagaagcgg cttctggctt ttctcgccac tcgtcgatcg ctacttccgt
     9961 gcgatgcgca aggcgctcta cctccttggc gatcgcctgg gtcagcggca tggccagtgg
    10021 atggtgccaa aggcgatcaa actccgcctg cacccagtcg caggcctcgg ggctgtcgtc
    10081 ttcccacata agttcgtagt tgagtgtcca tgcggcgagc gtctcattgg cactgcctaa
    10141 aaagctcgtg cgcctgccgt cggcataggt gataacgccc gccttcccgt ggatgaggcc
    10201 gaagacatcg tcggggagca cccgcacctc aagccgcgcg cgcccttccc ctcgcaggag
    10261 gcgcacgagc cgctcgagcc gctcagtgcc gctttgggcc agctcttctg gatcgtgctt
    10321 gaagaatgac agccgttgtg cctgttcgcg ggctgccgcg gaggctgcct caatgtcctg
    10381 ctgcctgata tcggaattgc agacgatgcg gatgctccca cgcacctgtt cgaaggcttc
    10441 acccgccagt tcgaagagcg agctgtcaaa atagccggca atccggtcat agcgcaccgc
    10501 gccttgaagc cgcgaggcga ggaactgggt gcctggctga cttcggctgc gctggaagac
    10561 atggcgctgg atcatggctg gagcctcttt cgaacatatt tctggtgcga acttcttggt
    10621 ctgtcggggt agagtaattc aagttgccca gaattcagga gtggcgttaa aatagtcacc
    10681 cgaaagtttt ctctatcttt gagtcccagc atatcttgta tttcttttct tgtccttgga
    10741 atctcacaga attcaaggac tgctgcaaat cttctctggg tttcctccga ggtgacttgc
    10801 tgggtgactt gctgggtgac ttgctgggtg acttgctggg t
//
LOCUS       DAOOWY010000063        10646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_6222_length_10646_cov_8.923331, whole genome shotgun sequence.
ACCESSION   DAOOWY010000063 DAOOWY010000000
VERSION     DAOOWY010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6222_length_10646_cov_8.923331"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="PK105_05915"
     CDS             <1..1553
                     /locus_tag="PK105_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU61281.1"
                     /translation="QEDIPCEGMYFSSGYAKAENGERYTFVWNEKKFPHAEGFIQNLR
                     NRGYRICCNVKPGILLDHPWYDELAKDGVFIPDNDGSPLRSYYWGNSASFVDFSRKEG
                     FDWWVKSLKKYILEKGISGVWNDNNEFEIEDKSLPIQAVKNILPIKMAEASYSALRSL
                     YPGKRPWLISRSGYAGLQRYARTWTGDNVSSYDTFRFNIAMGMNLGLSGLPFYGHDIG
                     GFVGPTPNEELLLRWCQSAVFQPRFVMHSWKPDGSITEPWLYPNRLSDIRNFIRMRYR
                     FLPYIYDLAIRASETGIPMESPVALEFPEDISLSLDSLDRMAGDAILVPAPPEQGKDE
                     VSVLLPKGINWFDPIHQIIYGGGEKLSFDYYPHDIQYFFRCGTVIPTFHKIEAIGKSL
                     LNSYEFIVIPPAEKSDIGLLPIACIHSEDDGESDFIPGSHWRWMMEYTVEGENDYCLH
                     IVQTEGVADKYDSSVFGKEWRFCVPDGFMLYDKNEMKMGTSLVFDLDKAPEHLSLRVK
                     GAYRNKHR"
     gene            1571..2560
                     /locus_tag="PK105_05920"
     CDS             1571..2560
                     /locus_tag="PK105_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU61282.1"
                     /translation="MVTIRDVAEHAGVSVSTVSHAISGKRPISQVTRQRIFQAIEQLG
                     YEPNPAARALRTKSTGVIGFFAFDITEVFAARIIHGVERIVREKNCFLLFTSGVGFNY
                     DLAQGIDFLRKRRVDGIIIAYGVRQEISPKTFDLSNIPLVTINTFVDLTIPSLHPDDF
                     QGGKEAALHLVSKQARHPAMIAGPENRLASKERLLGFQKGLEECGMPFDASERVMYGD
                     FGAESGSYCFDALLKACPDLDAVFCANDYMAAGAINRAITSGISIPQDMHIIGYDDRE
                     FAAFWPIPITTFAQPLEYMGELGATMLFERIDGHNPEPSMIKLAPTLIARQSS"
     gene            2686..4149
                     /locus_tag="PK105_05925"
     CDS             2686..4149
                     /locus_tag="PK105_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOU61283.1"
                     /translation="MASHDSRFFDSENKKDVFPFADNDNPLDLVIAYIGGGSRGWAWT
                     LMSDLALEGSLRGIVRLYDVDKKAAETNAQIGNALRERDDVLGKWRYEIAEDLESALR
                     DADFVIISILPGTFAEMASDVHSPEEYGIYQSVGDTTGPGGILRALRTIPMYEVIGRA
                     ICEFCPKAWVVNYTNPMALCLRALYEVFPDIKAFGCCHEVFGTRKLLAAMVRERLGSA
                     GIPDSRDATMQQIEVNVLGVNHFTWIDRASFGTVDLIPHYRAFVDEHFELGYEGAEGE
                     SWLNSYFASAERVKFDLFRRYGLIAAAGDRHLAEFMPGRMYLRDPNTVRSWKFSLTPV
                     SWRIENAERLRQKAANYAEGRDRFELKHSGEEGVKLIKALCGLGSLVTNANLPNHGQI
                     KGLPLGAVVETNALFQRDCVQPVDAGRLPPQIEALVAPHAIAQDIVLDAALRRNAWAA
                     FSAFLHDPLMTATPLEAEALFSSMLRATANFLPDYTL"
     gene            complement(4332..5588)
                     /locus_tag="PK105_05930"
     CDS             complement(4332..5588)
                     /locus_tag="PK105_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU61284.1"
                     /translation="MKRTSLALLIILMSAAMVFGQQQITIRWAYWGSESRVKRSQEAI
                     NLFEAANPGIKVNPEISGGTGDHFNKVDTQLAGGSGPDIIQMGGNINDYVKREVLLPL
                     EKYAGTILNTAVIDPSAIESGTIDGHLYGVSTGVTMPALVYNKSLIQRVGAPLPKVSM
                     TYEEFRNYLVMLKSKLPSGTYPMQDIGALSSNSTPFGYWTRYNGTPLYNAATKTTAVR
                     PADAQKYLELFKDYRNNGLIPPADIAAGYAETNADTSMVIAGKAAIGFIWTNQLAGYQ
                     AATTDELCLMEFPGAAATKALWQAPSQFYTVNKNSKHPEEAVKFINFLVNSPEAAKVL
                     GNDRGASASATARAAGAADINDRKVLEYLDVAGPHSSKETDHVPNDTEFNSTLFLIYQ
                     KVAFGQISTAAGGQQIYDLIVRLIKK"
     gene            complement(5620..6468)
                     /locus_tag="PK105_05935"
     CDS             complement(5620..6468)
                     /locus_tag="PK105_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU61285.1"
                     /translation="MKRRTTSLIKSILTNILILLLGMCMLYPVVWLIAASFKESNTIF
                     SDPGLWPKAFTLQNYVKGWQGIGIVGFATFFKNSFIISGLCVIANVIFCSLTAYAFAR
                     LKFAGRNIWFAIMMVTMMLPTHVTTIPRYIMFRKFGWIDTFLPLVIPKFFATDAFFIF
                     LLVQFIRSLPKELDESALIDGCGKFGIYTRIVMPLTKPALITTVIFTFLWTWDDFFNQ
                     LLYLNSPSKYTVPMGLRLFLDSSGISSWGPMFAMSVLSLLPCLILFFSLQKYFVQGIA
                     TTGIKG"
     gene            complement(6465..7391)
                     /locus_tag="PK105_05940"
     CDS             complement(6465..7391)
                     /locus_tag="PK105_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU61286.1"
                     /translation="MNERARKKAIRENLTSYAFLVPWLLGFFVLTLYPMIYSFFLSFT
                     EFNILQPPKWLGLRNYFVMFIGNEQFPRDERFLNSLLVTFKYVFISVPLKLIFALGIA
                     MLMNRDLKLIPFYRTLYYIPTLLGGSVAIAALWRRLFAGDGVLNMILRSAFNLNPPAW
                     ISNPKYALYTLILLSVWQFGSPMIIFLAGLKQIPKEYYEASAVDGAGKMRQFFSITLP
                     CLSPIIFFNLVMQMINAFQSFTQAYIISGGQGGPLDSTMFYSLYLYLKGFAFSEMGYA
                     SAMAWVLLIIIAALTALAFKVSGSLVSYGSGE"
     gene            7874..8767
                     /locus_tag="PK105_05945"
     CDS             7874..8767
                     /locus_tag="PK105_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOU61287.1"
                     /translation="MKRENIQIRDPFVLPVKDEMRYYLYGTTDSDPWKAPGIGFDAYI
                     SENLEEWEGPYPVFRPQSDFWASHNFWAPEVYKYGKSYYMFASFKSSSHERATQILRA
                     ASPFGPFVQHSNEPVTPASWECLDGTLWEEEGQPWLVFSREWVQVNDGEVWAAPLSID
                     LDRLDGEPILLFRGSDASWTRKIKRRNGSGFEDARVTDGPFLYKTDQGSLFMLWSSIG
                     ESGYAMGYARSESGKLIGPWLQQSKPLVDRGRGHGMIFSGFNGRTYLAYHFPNETPYE
                     RFCYREIEVLSTSIAFIGEEL"
     gene            8771..>9399
                     /locus_tag="PK105_05950"
     CDS             8771..>9399
                     /locus_tag="PK105_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61288.1"
                     /translation="MAIERQSLVRRHNPVLQKYDPFSPLSVGNGEFTFTADITGLQSL
                     ISDIPGITPLCTMSQWGFHSYPEREDCPRDEKRLRLSYFDPIRNTGGYMIDPTGQEEL
                     FNELRINPHRFNLARVGFVFEGEKLEARDISEIEQVLDLWKGELHSRFSYKDQPVDVF
                     TICDPEVDALAIRVFSPLLDQGRLCVRIAFPYGSHEIDASDWIAEQRHST"
     assembly_gap    9400..9499
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9500..>10646
                     /locus_tag="PK105_05955"
     CDS             <9500..>10646
                     /locus_tag="PK105_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61289.1"
                     /translation="RSASFRSIGYRAARHWEEFWSTGGAIELIESRDERAMELERRIV
                     LSRYLTAIQCAGSYPPAETGLTCNSWYGKFHLEMHYWHAAHFIVWGKPDLFERSFEWY
                     RSILPSARRRAHEQGYAGARWPKMTDPSGRDSPSLIGPLLCWQQPHPLMYIELLRRAY
                     LDRPVISEYRDIIGATADFMASFAQWNQSKQCYELGPPLIPAQENHKPEDTLNPSFEL
                     EYWRWGLSAAIRFFEMIDLPVNPHWREVASRLAPLPADMASHVYLAHENCPRTFDRFA
                     ADHPEMLFALGMLPGSSIDKTIMSDTLDMVLKTWDLESLWGWDFPAMAMTAFRLGRKK
                     DAIDLLLMETPKNTFRANGHNPQLPRTDLPVYLPGNGALLLAISLIAQ"
BASE COUNT         2902 a         2249 c         2524 g         2871 t
ORIGIN      
        1 aacaagaaga tataccctgc gaaggcatgt atttttcttc aggctatgca aaagccgaaa
       61 atggcgagcg ctatactttt gtatggaatg aaaagaaatt cccccatgcg gaaggtttca
      121 ttcagaatct cagaaacagg ggatatcgaa tttgctgtaa tgtaaagcca ggcatactct
      181 tggatcatcc atggtatgat gagcttgcaa aggatggggt ttttatacct gacaatgatg
      241 gctctcccct aagaagctat tattggggca acagcgcgtc attcgtcgat ttttcccgaa
      301 aagaaggttt tgattggtgg gtcaaatcgc taaagaaata tatcctcgaa aagggaattt
      361 cgggagtgtg gaatgacaat aacgaattcg aaatcgagga taagtctctt ccgattcagg
      421 ctgtcaagaa tattctgcca ataaagatgg cggaagcatc ttatagcgca ttaaggtcct
      481 tatatcctgg caaaaggcct tggctgatca gtcgctccgg atatgccggg cttcagcgct
      541 atgcccgcac atggaccgga gataatgtgt ccagctatga tacattccgc ttcaatattg
      601 caatggggat gaatctaggg ctcagtggcc taccattcta tggccatgac attggtggat
      661 ttgttggccc aacgccgaac gaggagctat tgctgcgttg gtgccagagt gctgtctttc
      721 agccacgctt tgttatgcac tcatggaaac ctgatgggag cataaccgaa ccatggctat
      781 atccaaaccg gctttcggat atccgcaatt ttatacgaat gcgataccga tttttgccat
      841 atatctatga tttagcaatt cgcgcaagtg aaactggcat tccaatggaa agccctgttg
      901 cgcttgagtt tccagaggat atttcgcttt cattagatag cctggatagg atggccgggg
      961 atgctattct tgtaccagcc ccgcctgaac aaggaaaaga cgaagtatcg gtgctgcttc
     1021 ctaaggggat aaattggttt gatccgattc atcaaattat atatgggggc ggagagaaat
     1081 tgagcttcga ttattaccct catgacattc aatacttctt cagatgcggt acggtaatcc
     1141 ccacgtttca taaaatagaa gcaattggca aatctctttt aaatagttat gaattcattg
     1201 ttattcctcc agcagaaaaa agcgatatcg gcctactacc tattgcttgt atccatagcg
     1261 aagacgatgg agaaagtgat tttattcctg gaagtcattg gcgatggatg atggaatata
     1321 cagtggaagg agagaatgat tattgtctcc acatagtgca gaccgaaggc gttgctgata
     1381 aatatgattc ctcagtcttt gggaaagaat ggcgattttg cgtacccgat ggttttatgc
     1441 tctatgacaa gaatgaaatg aaaatgggta cgagccttgt atttgatctt gacaaagccc
     1501 cagagcacct ttctcttaga gtcaagggag cctatcgcaa taaacataga taagatgata
     1561 ttctagatga atggtaacta ttcgcgatgt agcagagcat gcaggagttt ctgtctcgac
     1621 ggtgagccac gcaattagtg gcaaacgtcc tataagtcag gttacgagac agagaatatt
     1681 tcaagcaatt gagcaactcg gttatgagcc caatcctgcc gcacgtgctc ttcgaaccaa
     1741 gagcacgggg gttatcggtt tttttgcttt tgacattaca gaagtctttg ccgcaagaat
     1801 aattcatggt gttgagagaa tagttcgtga gaagaattgt tttttgcttt ttacaagtgg
     1861 tgtcggcttt aattatgatt tagctcaggg tatagatttc ttacgcaagc gaagggtcga
     1921 tggcatcatc atcgcttatg gtgtgcgtca ggaaataagc ccgaaaactt tcgatctttc
     1981 caatatacct ttggtgacaa taaacacttt cgtagatctt actattccat ctctccatcc
     2041 agacgatttc cagggtggaa aagaggctgc gttgcatctg gtttctaagc aagcacggca
     2101 tcctgctatg atagcaggcc ccgaaaacag gcttgcaagc aaagaacgcc ttttgggttt
     2161 tcaaaaaggt ctggaagaat gcggtatgcc ttttgatgca tcggaaaggg tgatgtatgg
     2221 ggattttggc gctgaatccg gttcttattg ctttgatgcc ttattgaaag cctgtcccga
     2281 tctggacgcg gtcttctgtg caaacgatta tatggccgct ggagcaataa accgagccat
     2341 aacttctgga atctcgattc cccaggatat gcatatcatt ggatatgatg atcgtgaatt
     2401 tgccgctttt tggcctattc caataactac atttgcgcag cccctcgaat acatgggaga
     2461 actcggtgca actatgcttt ttgaacgcat cgatgggcac aatcctgagc caagcatgat
     2521 caaactggcg ccaaccctca tagctcgtca atcgtcatag gattagattg cattggtggt
     2581 gcgagcctta tctttatcca cgattttttt gctcgtcctt ttcatttaag agcaaatgta
     2641 gtattgttgg gcacttacat ttaagttcta tgcaaggaat aatgaatggc cagtcatgat
     2701 tctcgctttt ttgattctga gaataagaaa gatgtctttc cttttgcaga caatgataat
     2761 ccattagatc tcgtcatagc ttatatcggt ggaggctcac ggggatgggc ttggacacta
     2821 atgagtgatc ttgccttgga aggatcgctt agaggaattg ttcggctcta tgatgttgat
     2881 aaaaaggcag ctgaaacgaa tgcgcaaatt ggaaatgctc ttcgagagag agatgatgtg
     2941 cttggaaaat ggcgatacga aatagcagaa gatctcgaga gcgccctgcg cgatgctgat
     3001 tttgtaatca tttctattct tccgggtacc tttgcagaaa tggcttcaga tgttcattcg
     3061 cccgaagaat atggaatcta tcaatctgtg ggcgatacaa cgggaccagg tggaattttg
     3121 cgagcgctcc ggactatccc aatgtatgaa gtgatcggaa gagcgatatg cgaattctgt
     3181 cctaaagcat gggttgtaaa ttacacgaat ccaatggcac tttgccttcg cgcactttac
     3241 gaagtctttc cggatatcaa ggcctttgga tgttgtcatg aagtatttgg gacgcgcaag
     3301 ctgctagcag cgatggttcg cgaacgcctt gggtccgccg gtataccgga ttcaagggat
     3361 gccactatgc agcaaatcga agtcaatgtg cttggagtaa atcattttac ctggatagat
     3421 cgtgcatcat ttggcacagt tgatttaata cctcactata gagcatttgt agatgaacac
     3481 ttcgaattag gatacgaagg tgcagagggc gaaagctggc tcaattccta ttttgcttcc
     3541 gcagagcgtg tcaaattcga tctatttcgg cgttatggtc tgatagcagc ggctggcgat
     3601 aggcatctag ccgaattcat gccaggaagg atgtacctgc gagatccaaa caccgttcga
     3661 tcgtggaaat tctctttgac acctgtatca tggcgtatag aaaatgccga gaggctcagg
     3721 caaaaagcag cgaactacgc tgaaggcaga gatagatttg aactgaagca ttccggggaa
     3781 gaaggcgtaa agctcataaa agctctctgc ggattaggtt cgcttgttac gaatgccaat
     3841 ttgccaaatc acggccagat aaaagggctt ccattgggag cagtggtgga gaccaatgct
     3901 ctttttcagc gcgattgcgt tcagcctgta gatgcaggaa gacttcctcc tcaaatcgaa
     3961 gctctggttg cgccccatgc cattgctcaa gatatagtct tggacgctgc tctgcgacgc
     4021 aacgcttggg cggcattttc tgcattcttg catgatcctc tgatgacagc gacgccttta
     4081 gaagccgagg ctcttttttc tagcatgcta agagcgacag ccaattttct gccggattac
     4141 acgctataaa aagatgaaaa gcccctagaa taaagagaat tatccagggg caatccaaag
     4201 agtgaactaa ttatagaaga agcaagaagg ggtgcagcat atccaaagcc gcgccccttt
     4261 cttagaactg ctcaaatatg gcccttcgag atagaacaag cattttcttt atgggctcca
     4321 tcaatcatgg ttcacttctt tatgagtctt actataagat catagatttg ttgtccgccc
     4381 gcggctgtag atatctggcc aaatgccacc ttttgataga taaggaacaa ggtgctattg
     4441 aattccgtat cgttgggcac atgatccgtt tcttttgaag aatgtggccc tgcaacgtcc
     4501 aggtattcta gaacctttct atcattgatg tcggctgctc ccgcagctct agcggtagcc
     4561 gaggcagagg caccacgatc atttccaagt acttttgccg cttctgggct attaacaagg
     4621 aagttaatga actttacggc ttcctctgga tgcttagaat ttttgttaac cgtgtaaaat
     4681 tggctcggag cctgccagag cgccttagta gccgccgcac cagggaattc catcaggcac
     4741 agctcatcgg ttgtggctgc ctgataacct gcaagctggt tcgtccaaat aaatcctatg
     4801 gcagccttac cggcaatgac catcgatgtg tcggcatttg tctctgcata acccgcggcg
     4861 atatctgccg gaggaatgag cccattgttg cggtaatcct taaagagctc gagatacttt
     4921 tgtgcatccg ctggtcttac ggcggtagtc ttggtcgcag cattgtagag aggagtgcca
     4981 ttgtagcgag tccaatatcc aaaaggagtc gagttggacg aaagagcccc gatatcctgc
     5041 attggatatg ttcccgacgg aagttttgat ttgagcatca caagataatt gcgaaattcc
     5101 tcataagtca ttgagacctt tggcagaggc gcaccaaccc tttgtataag cgatttattg
     5161 tagacaagcg ccggcattgt tacacccgtg gaaacaccat agagatgtcc atcgatagtg
     5221 ccagattcta ttgcacttgg atcgatcacc gccgtgttga gaatagtccc agcgtatttt
     5281 tccaggggaa ggagcacttc tcttttgaca taatcattta tgttgccacc catctggatt
     5341 atgtcagggc cactcccgcc agctagctgc gtatcgacct tattgaagtg atctcctgtt
     5401 ccacctgaga tttctggatt aactttgatg cccggattag ctgcctcgaa gaggtttatg
     5461 gcttcctgac ttcgctttac tcgggattcg cttccccagt aagcccaacg aattgtgatc
     5521 tgttgttgtc cgaataccat ggcagcgctc attaaaatta tgagcagtgc caaactcgtt
     5581 cttttcatga tctacctcct atcgaaagaa taaaaatact tatcccttaa tgcccgtcgt
     5641 agctattccc tgcacaaagt acttctggag cgagaaaaag aggatgaggc aaggaagcag
     5701 agaaagaacc gacatggcga acataggccc ccaagatgag ataccagagg aatcaaggaa
     5761 gagcctgagc cccatgggga ctgtgtattt agacggagaa ttaaggtaaa gcagctgatt
     5821 aaagaagtcg tcccatgtcc acagaaaggt gaaaatcacc gttgttatca gagctggttt
     5881 ggttagtggc ataacaatcc gtgtatagat tccaaatttg ccacaaccat cgattaaagc
     5941 cgattcatct agttctttgg gaagactgcg aatgaattgc acaagcaaaa aaatgaaaaa
     6001 tgcatcagta gcgaagaatt tgggaataac aagtggaagg aaagtatcta tccatccaaa
     6061 tttcctaaac atgatatatc gtgggattgt tgtcacgtgg gttggcaaca tcatagtaac
     6121 catcatgatg gcaaaccaaa tatttcttcc cgcaaactta agcctcgcaa atgcataggc
     6181 agtgagggaa cagaatatca catttgcaat tacgcataac ccggatatga tgaaggaatt
     6241 cttaaagaaa gtggcaaacc ctactatacc aattccttgc cagcccttga catagttctg
     6301 caaagtgaag gccttaggcc atagcccagg atcgctgaag atagtattgc tctccttgaa
     6361 tgatgcagcg attagccata cgactggata caacatgcac ataccgagca agaggatgag
     6421 gatatttgta agaatagact ttatcaagct cgtagtgcgc cttttcattc tccgctccca
     6481 tagctgacca gtgagcctga taccttgaag gcaagcgcgg taagagccgc gattatgata
     6541 agaagaaccc aggccatagc tgacgcgtac cccatctcag agaatgcaaa accctttaag
     6601 taaagataga ggctgtagaa catcgtggag tctagcggac cgccttgtcc acctgagatg
     6661 atataagctt gtgtaaaaga ctggaaagca ttgatcatct gcataacaag attaaaaaaa
     6721 atgattggcg ataggcaggg tagggtaata gagaagaatt gccgcatctt gccagctcca
     6781 tctaccgccg aagcctcata gtattctttt ggtatttgct taagacctgc aagaaaaatt
     6841 atcataggag atccaaattg ccacaccgat agcagaatca aggtatagag ggcatactta
     6901 ggattcgata tccatgctgg cggattgagg ttgaaagcgc tgcggagaat catattcaat
     6961 acgccatctc cagcaaagag ccttctccat aaggcagcaa tggcgacaga accacccaga
     7021 agggtgggaa tatagtagag agttcgatag aatgggatga gtttgagatc tcgattcatc
     7081 aacatcgcta tgcccaacgc aaatattagt ttaagaggaa cagagatgaa cacatatttg
     7141 aacgtaacga ggagagaatt cagaaatcgt tcatccctgg ggaattgttc atttccaatg
     7201 aacattacaa agtaattgcg caggccaagc catttgggag gctgaaggat attgaattcg
     7261 gtaaaggata gaaagaaaga ataaatcatt gggtaaagag ttagcacaaa aaagccaagt
     7321 aaccatggaa ctaggaaagc atacgatgtc aggttttccc ggatggcctt tttcctggct
     7381 ctctcattca ttttctttct cttctctgga tttatggctt aatccctcat acatcatctg
     7441 tagtgcaaag atgctcctga aaaccgattg acctcatcaa cgacaaaagc atatttctca
     7501 tttctattat taggatcctt ttgtgcaaaa tgccatgtaa aatattgaca atagatatct
     7561 attattgact tttttcatac ccctttgatt tcaaaagcag catcatatat atatataaat
     7621 atatgtatat tccggtcatg atcttttttg atgagcatga atttccaaat tgcttttaga
     7681 tgagggtata attatttctt gcataggcaa agagcttttc tgtatcgctc cggcaggctc
     7741 ataataagag aaaaagagca gcagattggc atttctaacg gaattgctct agaaaaacat
     7801 atctgttatt atgctaatat tcaataggat tgaagacttc aagcgcatag atatagatat
     7861 aagagagaaa gccatgaaga gagagaatat ccaaattcgc gatccatttg tgttgccagt
     7921 aaaagatgag atgcgatatt acctctatgg gactactgat agcgacccct ggaaagctcc
     7981 aggcattggc ttcgatgcat atataagcga aaatctcgaa gaatgggagg ggccatatcc
     8041 tgtttttagg cctcagtctg atttttgggc aagccataat ttctgggcgc cggaagtcta
     8101 caagtacgga aaatcctact atatgtttgc aagtttcaag tcatctagcc atgagcgggc
     8161 aacgcaaatt ctccgcgcag cttctccttt tggtccattt gtacaacatt caaatgagcc
     8221 ggtgactcca gcgagctggg aatgcttaga tggcactctc tgggaagaag agggtcagcc
     8281 ttggctggtc ttttctaggg agtgggtcca ggtaaatgat ggtgaagttt gggcagctcc
     8341 tctttcgatt gatctagatc ggctggatgg cgaacccatc ttgctttttc gaggttcgga
     8401 tgcgtcctgg acaaggaaaa ttaaaagacg aaatggctca ggctttgaag atgctcgagt
     8461 gaccgatgga ccctttcttt acaaaacaga ccagggatcg ttattcatgc tctggtctag
     8521 tattggagaa tcgggctatg caatgggata tgctcgctct gaatcaggaa agctcattgg
     8581 accgtggctt cagcaatcaa agccccttgt cgatagaggg cgaggccatg gcatgatttt
     8641 ttctggcttt aatggaagaa catacctggc ttaccatttc cccaatgaaa ctccctatga
     8701 gcgcttttgt tatcgggaga tagaagtatt gagtacaagt atcgctttta ttggcgagga
     8761 gttgtagatc atggctatag agcgccaatc tcttgttcgt agacataatc cagttcttca
     8821 aaagtatgat ccattctctc ctctatcggt tggcaacggc gaattcacct ttactgcgga
     8881 tatcaccggc ttgcagagtc ttatttccga tatacctgga attacgccac tttgtacgat
     8941 gtcgcaatgg ggattccatt cttatccgga acgcgaagat tgccctcgcg acgaaaaaag
     9001 gctcaggctg agctacttcg atccaattag aaatactggc ggctatatga tcgatccaac
     9061 agggcaggaa gagttattta atgagcttcg aatcaatcca cacaggttta accttgctag
     9121 agtcggtttt gtctttgagg gggaaaagct ggaagctcgg gatatatcag aaatcgagca
     9181 ggttctcgat ctatggaaag gtgagctgca tagccgattc tcttataagg atcagcccgt
     9241 agatgtcttt actatctgtg atcctgaagt ggatgcgtta gctatccgtg tcttttctcc
     9301 acttcttgac cagggtcgtc tttgtgtgcg tattgctttt ccctatggtt cgcacgaaat
     9361 tgatgcttct gattggattg cagaacagcg ccatagcacn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnng cgctcagctt cttttcgttc tataggctat agagccgctc
     9541 gacattggga ggaattctgg tctaccggag gggccataga gctaatcgag agcagagatg
     9601 agagggcaat ggagctcgag cggaggatag ttctttcccg ataccttacc gcgatccaat
     9661 gtgctggatc ctatccgccc gcagagacgg ggcttacctg caatagctgg tatggcaaat
     9721 ttcatcttga gatgcattat tggcatgcag cgcacttcat tgtatgggga aaacccgacc
     9781 tattcgaacg aagtttcgaa tggtataggt cgattcttcc ttccgcaaga aggcgggcgc
     9841 acgaacaagg gtatgcaggt gctcgatggc caaagatgac tgatccatcg gggagagata
     9901 gcccgtcgct aattggaccg cttctatgct ggcagcagcc tcatccgcta atgtacatag
     9961 aactcttgag aagagcttat ctggatcgac cggttatttc cgaatatcga gatatcatcg
    10021 gagcaacggc ggactttatg gcgagctttg ctcaatggaa ccagtctaaa cagtgctatg
    10081 aacttgggcc gcctctcatt cccgcgcagg aaaaccataa gccggaagac acactcaatc
    10141 caagctttga gctggaatac tggcgttggg gtttatctgc ggccattcgt tttttcgaga
    10201 tgatagatct gcctgtgaat ccccattgga gagaagtagc aagtagactg gctccgcttc
    10261 cggccgacat ggcaagtcat gtatatctcg cacatgaaaa ctgtcccagg acttttgatc
    10321 gatttgcggc cgaccacccg gagatgcttt ttgcccttgg aatgctgccc ggctcttcca
    10381 tagacaaaac tataatgtcc gatactctag atatggtatt aaaaacctgg gatctggaat
    10441 ctctttgggg ctgggacttt cccgcaatgg ccatgacagc ttttcgcctg ggaaggaaaa
    10501 aggacgcaat cgatcttctt ctcatggaaa cacccaagaa cacctttaga gccaatggtc
    10561 ataatcctca gttgccacgt accgatctac cggtttatct gcctggcaat ggtgcgcttt
    10621 tattggctat ctcgctaatt gcccag
//
LOCUS       DAOOWY010000064        10203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_6573_length_10203_cov_10.301537, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000064 DAOOWY010000000
VERSION     DAOOWY010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6573_length_10203_cov_10.301537"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1117)
                     /locus_tag="PK105_05960"
     CDS             complement(248..1117)
                     /locus_tag="PK105_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOU61290.1"
                     /translation="MQESSKKLGYTEGIASIIINTMLFGLKIWVGTASSSVAMTADAW
                     HTLSDTLTSIVVIFGFWFSSRPEDEKHPFGHGRAELIATIIIATLLAVVGLNFLRDSI
                     QQLIIRKSANFSTLGLVVFAVSVVIKEGLARFSIWAGKKTNSQSLLADGWHHRSDAIA
                     SLIIVIGAVLGKYLWWIDGVLGIGVSGLVLYAAYDIAHTASDSLMGEKTSDDLTKRIK
                     RIASEASPELKDIHHIHIHRYGDHLEITLHARLDKKTDIEDAHALSTRLEQRLRNELR
                     ADTTVHIEPVKEA"
     gene            complement(1354..2166)
                     /locus_tag="PK105_05965"
     CDS             complement(1354..2166)
                     /locus_tag="PK105_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU61291.1"
                     /translation="MKRYIRKILFFILIIAIVVPLLFPFVWMLSSSFKTQVDIVAWPP
                     KLIFSPTLQNYKRVFSEQNFLKYFVNSSIIGISSVFFSLLMGLPAAFSIARFGQKKLS
                     IFILIARLMPGISFLMPWYIIFSRLRLMDSYVALILSHMLITLPIIVWIMESYFETIP
                     LEIEESAMVDGATRQRAFLAIVLPIAAPGIVTSITLSFIFSWNNFMFAQVLSMERTKT
                     LPLAIYNFVSYAEVDWGAVMAAAVAIMAPAIILTMIFQKYVVQGLTMGAVKG"
     gene            complement(2186..3076)
                     /locus_tag="PK105_05970"
     CDS             complement(2186..3076)
                     /locus_tag="PK105_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU61292.1"
                     /translation="MVSKRNRGSFIEKNLHIIFPLPAVVFIIVMMLFPVLYTFFMSFT
                     NWNLTSGMPLSFVFLSSYKRVLQEPRFIKALGRTFSFTALAVSIETVFGMVIALILNR
                     EFKAKGFTKLIMLLPLVSTPVAIGIAWNLFYDPTIGLMNFMLTQLGLPKLAWTGSAST
                     VIPSLALVDIWQWTPMISLIMLSGLASLSNEPYESARVDGATEWQIFWHITLPMVMPT
                     LLTAIILRTIDALKTFDIIYAMTGGGPGYASETLNIMAFKYSFEYFRMGQASVVLIIL
                     FLLVLAMSLLIVRMRKSLEL"
     gene            complement(3139..4389)
                     /locus_tag="PK105_05975"
     CDS             complement(3139..4389)
                     /locus_tag="PK105_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU61293.1"
                     /translation="MKRKLMLVLVALLALFAVSAQQKEIRVLLANHPYGDLLKSAIPE
                     YEKATGVKVNVESLQESQLTTKLTTEFATRSSTVDVFMTRPLQEGKMFYKNGWYEPIT
                     GYDFSDYPKNAMDVASFGNKAYIVPLVTEWEVLYYRIDLLKKAGITVPTNFAELEAAA
                     KKLHSGDIVGFASRGKGAAAVTQLSSYVYNYGGAYLDKGKAVFDSKEALEAIRFYGRM
                     LANYGPAGVTNMSWENIMPLFQAGKIAMWTDASVFYGQIVDPSKSQVPAENVGIANFP
                     AGPKGNTPFIVVSWGMSIAKQSKNKELAKDFIKWATSKDLAKRGMLANITMARGSVWK
                     DAEVRAKIHPSLIATQEYAAKYGIPYDRPYMSAVGEARDLIGEVVIESINTAGTSPKL
                     EALAKEKAAAVNALLEDTGEYGVY"
     gene            4613..5464
                     /locus_tag="PK105_05980"
     CDS             4613..5464
                     /locus_tag="PK105_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOU61294.1"
                     /translation="MERSAAPSCLYLIHSLPNSLSERDRRIADYILNDPAKAVHLSIE
                     ELAEAAQVSVSTLVRFVKKLGFRGYQQFRIALASEALAPEARIYETIVDSGEDPVRLA
                     FSSASKALEITSSMIDSQDLYKLASRIIEANCVYLFGLGGSLIVAKDALHKLVRTGIR
                     CIGAEDYHMQLMIASQMNQQDTAIIVSHTGANKDAIGLAETIKTTGAFLCVITTYPRS
                     ALSRMADMRFISASSGSQAISEAFSARIAQLALIDSLYIAIMKQLGEEGIKNVEKMRA
                     TIAKRRI"
     gene            5481..6932
                     /gene="gnd"
                     /locus_tag="PK105_05985"
     CDS             5481..6932
                     /gene="gnd"
                     /locus_tag="PK105_05985"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOU61295.1"
                     /translation="MKADIGLIGLAVMGENLVLNMESHEFTVAVYNRTVEKVDSFLKE
                     RGAGKRILGAHSPKELVEMLKKPRIVMLMIRAGTAVDEMIAQLIPFLEPGDIIVDGGN
                     SNYEDTMRRFSELESKGFLFVGTGISGGEEGALTGPSIMPGGSEAAWPHVKPIFQAIA
                     AKVDNQSPCCEWVGPSGSGHFVKMVHNGIEYGDMQLISEVYQIMRDRLSMSPDEMAMV
                     FDEWNKGELDSYLTSITSDILRYKDEDGTPLIEKILDAAGQKGTGKWAGINALNFAVP
                     LTLIVEAVFARCISAMKDERLRAVKVLGRPTGTEYSGSHEKALKDLGAALYAAKIISY
                     AQGFMLMREASTAYDWKLNYGSIALMWRGGCIIRSRFLGRIKEAFDVDPGLSNLVFAP
                     FFAERLLKNEKAIRNVVTEAVAGGIPVPALSSALAWFDALRTERLPANLIQAQRDYFG
                     AHTYERIDKPRGQFFHTNWTGHGGSTSASTYIV"
     gene            6983..8068
                     /locus_tag="PK105_05990"
     CDS             6983..8068
                     /locus_tag="PK105_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOU61296.1"
                     /translation="MNPIRLLPEEDVKLDLLSLGGLVMRMDPGIVPFAFAENYSVHVS
                     GGEYNVAANLARCFGKRTAIATAIVDNPLGEKIAAEVRKMNVLGIYKHFKSDGVRGPN
                     MALVWSDRGHGVRPPVVFYNRSNEAAALLKPNDFDFEPIFKEGVRWFHSGGIFAALSD
                     STPQLIIEAMKIAHDHGALVSFDLNYRAKLWAAANTGEKHSDVFGRIVEHVDVLLGNE
                     EDLQMGLGLPGPDIHAVSKLDPSSFLKTIEQVHQRWPNIKAVATTLRDVKSTNRHLWS
                     AVLWIEGNHWIAPTMELDIYDRVGGGDGFASGLIYGLLEGEDPEEALRLGWAHGALLT
                     TYPGDTTMASLEQVKALARGQSARIQR"
     gene            8098..9132
                     /locus_tag="PK105_05995"
     CDS             8098..9132
                     /locus_tag="PK105_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOU61297.1"
                     /translation="MKAMILKEYKHLEIKDIPMPHIESADEVLIRVKATAICGSDIHG
                     FDGSTGRRQPPLVMGHEASGVVESVGPEVKTLKIGDRVTFDSTIWCGQCYFCRQGQVN
                     LCDARRVLGVSCDEYRQDGTFAEYVIVPERIVYRLPDNVAFEEAALTEPVSVAMHAFE
                     ITDMKPGESAAVVGTGLIGLLLIQIIRAYSPHPILAFDTDEKRRKAAIEAGADVALDP
                     QDPDVHSTVLSMTNDRGVDRAFEAVGASAAIGTAIESVRKGGSVTLIGNISSKAEIPL
                     QSVVTRQISLLGSCAIAGEYPISLALMANRNINVRQVISNIVPLEEGPLWFDRLYKRE
                     PGLLKIVLTP"
     gene            9231..>10203
                     /locus_tag="PK105_06000"
     CDS             9231..>10203
                     /locus_tag="PK105_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU61298.1"
                     /translation="MHNKVRFGIIGYGKVAALHAKALETTEHAELVSVSGHRQERRDA
                     FAHQWGLNSRNSVEEMIKEDGIQAVVIATPHLQHYSDAFSALNAGCHVLVEKPLALSV
                     AEAETLIIEANKRNLLLSVIAQRRWYPSCKRIRHAIDEGYLGTPALAQLTILGWRDEA
                     YYSSDPWRGSWEHEGGGVIVNQAPHQFDLLCWYMGEVAEVYGRWANINHPYIEVDDSA
                     VATVLFKSGGLASIFVSNSQRPGIYAKVHIHGSSGASAGVQTDGGAMFIAGRSSVLEA
                     PYNDIWTIPGQEECRAQWQKEDEEFFSHIDATWYFFAQQEEDFARAIL"
BASE COUNT         2856 a         2276 c         2418 g         2653 t
ORIGIN      
        1 taacctaaag agcgctcgat gaaactacca aatctatcga tagttgtcaa gtgctccaaa
       61 agctatctct atcttatttt tcgctttctc gccattacgc tcgtggcatg aagctccatt
      121 attattataa cgattttgag caaaaaagtc tctatactct atagcagact aatgcttaga
      181 cagcatttct tccattatta gacattcaag gaaactgtga aaagcctgga ccatttgcca
      241 tagctcttca agcttctttt accggctcga tatgcacggt cgtatctgct ctgagttcat
      301 ttcttagtct ctgctcaagc cttgtagaaa gcgcatgtgc atcttctata tcggttttct
      361 tatcgagcct tgcatgcaga gttatctcta aatgatctcc gtatctatgg atgtgtatat
      421 gatgaatatc cttaagttcc gggctggcct cacttgcaat tctcttgatt ctcttagtca
      481 aatcatcgct agtcttctca cccataagag aatcagaagc agtgtgagcg atgtcataag
      541 ctgcatagag aaccaggcct gatacaccta tgcccaggac tccatcaatc caccatagat
      601 atttccctag cactgctccg ataacaatga tcaaagaggc gattgcatcg ctcctatgat
      661 gccagccatc ggcgaggaga gattggctat ttgttttctt tccagcccaa atcgagaatc
      721 tcgcaagccc ttccttaatt acaacagaga ccgcaaacac cacaagacct aaggtactaa
      781 aattcgcgct ttttcggatt atcaactgct gaattgaatc tctaagaaag ttcaggccaa
      841 caactgctaa aagagtagca attattatag tagcaatgag ttcagctctt ccatggccaa
      901 aaggatgctt ttcgtcttca ggacggctgg agaaccaaaa accaaatatc actactatgg
      961 aggtaagagt atccgataga gtatgccatg catcagccgt catggctaca ctagacgaag
     1021 ccgtgccaac ccatatcttg agcccaaaaa gcattgtatt gatgattatt gatgctattc
     1081 cctcggtata accgagcttc tttgatgatt cctgcatgta gaacctccaa aattgaaaga
     1141 catctgctat atcgttttcg catatccgca gatatttcca ttggtcccgc aggaataatg
     1201 tcccgctgcg caaaagcccg gtcaactctt ttcgacaaga gaaacctgat ccatctatta
     1261 ttgcatgtat atgccaccgg tctatctagg tcaagtagca aaatacacat aggattcaaa
     1321 gagtctatag ctataatagg ctggctgtct ggcttatccc tttaccgcac ccattgttag
     1381 gccttggaca acatacttct ggaaaatcat ggtcagaata attgcaggag ccataattgc
     1441 cactgcggcg gccattactg caccccaatc gacttccgca taggagacaa agttgtatat
     1501 tgcaagtggc agtgttttcg tcctttccat gctcaaaacc tgggcaaaca taaaattgtt
     1561 ccaggagaat ataaaggaga gtgttataga agttacgatt cctggagccg ctataggcag
     1621 aacaattgca agaaatgctc gttgcctggt tgctccatcc accatcgccg attcttcgat
     1681 ctcaagcggt attgtttcaa aatagctctc catgatccag acgataatcg gaagagttat
     1741 caacatatgc gaaaggatga gagcaacata gctgtccatg agccttaatc tcgagaagat
     1801 tatataccag ggcatcaaga aagatattcc cggcatgagg cgcgctataa gaataaagat
     1861 cgaaagcttt ttctgaccaa acctggcaat tgagaatgcg gctggcaatc ccataagaag
     1921 agaaaagaat accgaggaaa tccctataat tgacgaattg acgaagtatt taagaaaatt
     1981 ctgttcgcta aaaacccgtt tgtaattttg caatgtcgga ctgaatatca actttggcgg
     2041 ccatgccacg atgtctacct gagtcttgaa tgaagaagag agcatccata caaaaggaaa
     2101 aagcagcgga acaacaatcg caatgattag aataaaaaat aatatctttc ttatatatct
     2161 tttcatccaa aacctcgttc tatcattaca gttccaaaga tttccgcatt ctaacaatca
     2221 agaggctcat agcaagcacc aataagaaga gaattattaa cactactgaa gcttgtccca
     2281 ttcgaaaata ctcaaaacta tacttgaacg ccatgatatt gagcgtctca gaagcatagc
     2341 ccggccctcc acctgtcata gcatagatga tatcgaaagt ctttagcgca tcgattgtgc
     2401 gcaaaataat ggctgtcaaa agagtgggca taaccatagg cagagttatg tgccaaaata
     2461 tctgccattc cgtggctcca tcgactcgtg ctgattcata aggctcatta gacaagcttg
     2521 ctagccccga gagcatgata agagatatca tcggtgtcca ttgccagata tcgaccaacg
     2581 ccagagatgg aataaccgtt gaggcagatc ctgtccatgc cagcttcggc aagcccagct
     2641 gagtaagcat gaagttcata agccctatgg ttggatcata aaagaggttc catgcaatac
     2701 caatagcaac aggagtactg acgagaggca gaagcatgat tagctttgta aaaccttttg
     2761 ccttgaattc gcggtttaga atgagagcaa tcaccatgcc aaacaccgtc tcgatagaga
     2821 cagccagagc cgtaaaggaa aaagtcctgc caagagcctt gataaatcgc ggctcttgca
     2881 ggactctttt ataggagctc aaaaaaacaa aggagagtgg catccccgag gtgagattcc
     2941 agttcgtaaa actcataaaa aacgtgtaga gtacgggaaa cagcatcatt actatgatga
     3001 aaaccacagc gggaagcggg aagattatat gtagattctt ctcgatgaag cttcctctat
     3061 ttcgcttaga aaccatgatc tatttcacct cggggactaa aataatagct ctatcagaaa
     3121 acagtttctg atcgctcgtc aatacacacc atattctccg gtgtcttcca ggagagcatt
     3181 tactgcggca gctttttctt ttgccagtgc ttcgagcttg ggcgatgtgc cggccgtgtt
     3241 gatcgattcg attacgactt ctcctattag atcacgagct tcgcccaccg cactcatata
     3301 ggggcgatca tacggaatac cgtatttcgc agcatattct tgtgtagcta taaggctggg
     3361 atggatcttg gctcgaacct ctgcatcctt ccacacagaa ccgcgagcca tggttatgtt
     3421 agctagcatg ccacgttttg cgagatcctt gcttgtcgcc cacttgatga agtctttggc
     3481 caactctttg ttttttgact gcttggcgat actcataccc caggaaacca caatgaaggg
     3541 tgtgttgcct ttcggtcctg ctggaaagtt ggctatgcct acattctccg cgggtacttg
     3601 gcttttagag ggatctacga tctggccata gaagacagaa gcgtctgtcc acattgcaat
     3661 tttgccagcc tggaaaagtg gcatgatatt ttcccaggac atattggtta caccggccgg
     3721 cccataattg gccagcattc tcccatagaa acggatagcc tccagggctt ccttgctgtc
     3781 gaaaactgct ttgcctttgt ccagatacgc tccgccatag ttgtacacat agctcgagag
     3841 ttgggtcact gcggcagcac ctttccctct cgatgcaaaa cctacgatat ctccagaatg
     3901 tagctttttc gccgccgcct ccagttcggc aaagtttgtt ggaactgtaa tgccggcttt
     3961 cttgagtaga tctatgcggt agtaaagcac ttcccactcg gtgaccagag gaactatgta
     4021 agctttgtta ccaaaagaag ctacatccat cgcatttttc ggataatcgg agaaatcata
     4081 tccggttatt ggctcatacc agccattctt atagaacatc tttccttctt gtagaggtct
     4141 cgtcataaag acatccacag tggaagacct ggtcgcgaat tccgttgtca gttttgttgt
     4201 gagctgactc tcttgaaggc tttccacatt gacctttact cccgttgctt tctcatattc
     4261 cggaatggcc gatttaagca gatcgccata ggggtggttt gccagcaaga ctcggatctc
     4321 cttttgttga gcgcttacag caaatagagc aagcagagcc actaagacaa gcatgagttt
     4381 tcttttcatc atgacctcct aaatagaaat gtgttgcaat tcgataataa tccgccctcc
     4441 agtctgagct tgtttcaggc caggcggatt tgacaacttt aatactaaga gccaaaagag
     4501 cattttgtca acacatgaag gtaatttttt ttcaagtgaa tggaaatttt tgccagtcct
     4561 ctgtacttat ccgagaatct aatataagat agggaaactt gatggtattt ctatggaaag
     4621 atccgctgcg ccgagctgtt tatatctgat ccactcgctc ccgaactcac tgagcgagcg
     4681 agatagacgc atagcagatt atattctgaa tgatccagcc aaagcagtgc atctcagcat
     4741 cgaggagtta gcggaagctg cccaggtttc ggtctctaca ctggtacgct tcgtaaaaaa
     4801 gctaggtttt agaggctatc agcaattcag aatagctttg gcttctgaag ctctggcacc
     4861 agaggcaaga atctatgaaa ccatagtcga tagcggagaa gatccggttc gcttggcttt
     4921 cagctcagcc tcgaaggcgc tcgaaatcac ttcatctatg atagacagcc aggatttgta
     4981 taaattggct tcgcgaatca tcgaagccaa ctgtgtctac ctttttggat taggtggatc
     5041 tttgatagtt gcaaaagatg cattgcataa gcttgttcgc acaggaatcc gctgcatagg
     5101 tgccgaggat tatcatatgc agctcatgat agcttcacag atgaaccaac aggatacagc
     5161 catcatagta tcccataccg gggctaataa ggacgccatc ggacttgccg agacgatcaa
     5221 gacaacgggg gcttttctgt gcgtgattac cacttatcca cggtccgctc tatccaggat
     5281 ggctgatatg cgcttcatat cggcctcttc ggggtctcaa gccatatcgg aagccttttc
     5341 tgccagaatc gcgcagctcg cgctaatcga ttcgctctat atcgcaataa tgaagcagtt
     5401 gggagaagaa ggcataaaga atgtggaaaa aatgcgcgct accatcgcaa agcgtcgtat
     5461 ctgatcatag aggaggattt atgaaagccg acataggatt gattggacta gcggtgatgg
     5521 gcgaaaacct tgtactgaat atggaaagcc acgagttcac tgtagcagtc tacaaccgaa
     5581 cagttgaaaa agtggattct ttcttaaagg aaagaggagc gggaaagcga attcttggcg
     5641 cccattctcc aaaagagctc gtagaaatgc ttaagaaacc aagaattgtt atgctaatga
     5701 tacgagctgg cacggctgtc gatgagatga tcgcacagtt gatacccttc ctggagcctg
     5761 gtgacatcat cgtagatgga ggaaactcaa attatgagga tacgatgagg cgcttttccg
     5821 agcttgaatc aaaaggcttc ttatttgttg gtaccggaat ctctggaggt gaagagggag
     5881 ctcttaccgg cccttcgatc atgcctggag gttccgaagc agcatggcct catgttaagc
     5941 caattttcca ggcgattgca gcaaaagtgg ataaccaaag cccttgttgt gaatgggttg
     6001 gaccaagcgg atctgggcac ttcgtcaaaa tggtccataa tggtatcgaa tatggagaca
     6061 tgcagctcat atctgaggtc taccaaatta tgcgagatag actaagcatg agtcccgatg
     6121 aaatggcgat ggtcttcgac gaatggaaca aaggagagct cgatagttat ctaacttcta
     6181 ttacaagcga tattcttcga tataaagatg aagatgggac ccctctaata gaaaagatac
     6241 tcgatgcggc aggacaaaag ggaacgggaa agtgggcggg aatcaacgct ctcaattttg
     6301 ctgtaccgct tactttgatc gtcgaagccg tatttgcccg ctgtatctct gctatgaagg
     6361 atgagcgact tagagcggta aaagtgctgg gaagaccgac cggtacagaa tactcaggct
     6421 cacatgaaaa agccttaaag gacttgggtg cagcgctata tgcggcgaaa atcatctcat
     6481 atgctcaggg ctttatgctg atgcgtgagg cttcgacagc atatgattgg aaactaaact
     6541 atggctcaat tgctcttatg tggagaggtg gttgcatcat acgttcgcga ttccttggta
     6601 gaataaaaga agccttcgat gtagatccgg ggctgagcaa tctagtgttt gctcctttct
     6661 ttgccgaacg gcttctcaag aatgaaaagg ccataagaaa tgtggtaaca gaggctgtgg
     6721 ctggaggaat accggtacca gctctatctt ctgccctcgc ttggttcgac gcgcttagaa
     6781 ccgagagact gcccgcaaat ctaattcaag cacaacgaga ttatttcggt gcacacactt
     6841 atgagcgaat agacaaacct cgcggtcagt tctttcatac aaattggacg ggtcatggag
     6901 gttctacatc tgcgagcaca tatatagtct gatattcgcg tatttacgag acataattat
     6961 tcaatatgag gagattgaaa gcatgaatcc aatcagattg cttccggaag aggatgtgaa
     7021 gctggacctg ctatccctgg gaggccttgt catgaggatg gatcctggca tcgtgccttt
     7081 tgcctttgcc gaaaattata gtgtccatgt ctctggcgga gaatataacg tagctgccaa
     7141 tctggcgcga tgctttggaa agagaaccgc aattgcaact gctatagtcg ataatccatt
     7201 gggggagaag atcgccgcag aagtccgaaa aatgaacgtc ctaggtatat ataagcattt
     7261 caaatccgac ggagtgcgag gcccaaacat ggctcttgta tggtcagatc gaggtcatgg
     7321 agtccgtccg cctgtagtat tctacaatcg ttccaatgaa gcagcagcct tattgaaacc
     7381 gaacgatttc gattttgagc caatcttcaa agaaggcgta aggtggttcc attctggagg
     7441 catcttcgca gctttatccg actcaactcc tcagctgatt atcgaagcta tgaaaatagc
     7501 tcacgaccat ggagccctag tatctttcga cctcaattac cgagctaagc tctgggctgc
     7561 ggcgaataca ggcgaaaaac actcagacgt gtttggtaga atcgtggaac atgtcgatgt
     7621 tcttttgggc aatgaagaag atttacagat gggactgggt ttgccgggac cagatataca
     7681 tgccgtatcc aagctcgatc cctcttcctt ccttaagacc atcgagcaag ttcaccaaag
     7741 atggcctaat ataaaggctg tcgcaacaac attgcgcgat gtcaaatcga ctaatcggca
     7801 tctttggagc gcagtgctct ggatagaagg aaaccattgg attgctccta caatggagct
     7861 cgatatctac gatcgcgtgg gcggcggaga tgggtttgct tctggtctaa tttatggact
     7921 gctggaaggg gaagatccag aagaagcact tcgactggga tgggctcatg gtgcgcttct
     7981 gactacttat cctggcgata cgactatggc aagtctcgag caagtcaagg cacttgcacg
     8041 tggccaatcc gcacgcattc agagatagcg gaatcgcatt ggtaagaagt gacagctatg
     8101 aaggcaatga tattgaaaga atacaagcac ctggaaataa aagatattcc gatgccacat
     8161 atcgaatcgg ccgatgaggt gctcattcgg gtaaaggcaa cggcaatttg tggttctgac
     8221 attcatggtt ttgacggttc aaccggaagg agacagcctc cgcttgtcat gggacatgaa
     8281 gcttccggcg tagttgaatc agtggggcct gaggttaaga cactcaagat aggagataga
     8341 gtaacctttg attcgacaat ttggtgtggc caatgctact tttgcagaca ggggcaggta
     8401 aatctgtgcg acgcaaggcg cgtacttgga gtctcctgtg atgaataccg acaggatggc
     8461 acatttgcag aatatgtaat tgttcctgaa agaattgtgt atagattgcc ggataatgta
     8521 gcatttgagg aagccgcact cacggagccc gtcagtgttg ctatgcacgc gttcgaaatc
     8581 acagacatga aaccaggaga aagtgcggca gttgtaggca cagggcttat tggactgttg
     8641 cttatccaga taatccgcgc atattcgccg cacccaatac tagcgttcga cacggatgaa
     8701 aagcgaagga aagcggccat agaggcagga gccgatgtcg cgctcgaccc tcaagatccg
     8761 gatgtccata gcacggtgct ttcaatgaca aacgataggg gtgtcgaccg agcgtttgag
     8821 gcggttggtg caagtgctgc tattggtacg gctatagaaa gtgtacgaaa gggaggttcg
     8881 gtgaccctaa ttggcaatat cagcagcaag gccgaaattc cattgcagtc ggttgtgaca
     8941 cgccaaattt ccttgctggg ttcttgtgcg attgctggcg agtatccaat ttcccttgca
     9001 ttgatggcga atcgaaacat caacgtcagg caggtaataa gcaacattgt tcctctcgaa
     9061 gaaggtccgt tgtggtttga tagactatat aaaagagaac ccgggcttct aaagattgta
     9121 ttgactcctt gatgagtgga tcgaattccg cgttattctt gttgaccgca ttaggcgggt
     9181 ttcataacat attctcaaat atagggttcc cccatccata ggagaaagac atgcataata
     9241 aggttcgatt tggaataatc ggatatggaa aagtcgcggc actccatgca aaggcactgg
     9301 aaacaactga gcatgctgag cttgtatctg tgagcggtca tagacaggaa aggcgagatg
     9361 cttttgccca tcaatggggg ttaaattctc gcaatagtgt agaagagatg ataaaagaag
     9421 acggcattca agctgtggtg attgcaacac cccatctcca gcactattca gatgcatttt
     9481 cagcgctcaa tgcaggctgc catgtgcttg tagaaaaacc acttgcgctc tcggtagctg
     9541 aagccgagac attgattatt gaggcaaata agcgaaatct gcttctcagt gttatagcgc
     9601 agcggcgctg gtatccatcc tgcaagcgta ttcgtcatgc aatagacgag ggatatctcg
     9661 gcaccccggc acttgcccag ctcacgatcc tgggctggcg agatgaggct tattatagct
     9721 ctgatccatg gcgaggctcc tgggaacatg aaggcggcgg agtaattgtc aaccaggccc
     9781 cccatcagtt cgatctttta tgttggtata tgggagaggt ggcggaagtc tacggtagat
     9841 gggcgaatat caatcatcct tacatagagg tcgatgattc ggcagttgca acggttcttt
     9901 ttaaatctgg aggtcttgca tcgatatttg tttcaaactc acaaaggcca ggaatttatg
     9961 cgaaagtcca tattcatggc agttcgggcg cctctgcagg agttcaaacc gatggtggag
    10021 cgatgttcat tgccggtcgc agtagcgtac tagaagcacc atataacgat atttggacca
    10081 ttcctggaca ggaagagtgc cgtgcacagt ggcaaaaaga agacgaggaa tttttttcac
    10141 acatcgatgc aacatggtat ttcttcgcac agcaagaaga agattttgct cgcgccattt
    10201 tgg
//
LOCUS       DAOOWY010000065         9843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_6870_length_9843_cov_11.906416, whole genome shotgun sequence.
ACCESSION   DAOOWY010000065 DAOOWY010000000
VERSION     DAOOWY010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9843
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6870_length_9843_cov_11.906416"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK105_06005"
     CDS             complement(<1..192)
                     /locus_tag="PK105_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61299.1"
                     /translation="MKIPSKGVHFKAANDRCNKENLGLPTGPFHAGIDYSYGNLARDT
                     TNNENASQLVVEMVPGLDLI"
     gene            361..>1757
                     /locus_tag="PK105_06010"
     CDS             361..>1757
                     /locus_tag="PK105_06010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU61300.1"
                     /translation="MFIKIGRLIAVLCIWGFALSALPAQNILDPSWSGVADEIRPKIQ
                     QRMSESDIKGLMIVLVDEEKTVWSEGFGFSDISTNKKVDADTMFEIGSVSKTFSGLMV
                     MQLYEQGKLDIDKPLVTYIPEFKLMPPVGNFPRVDRPITVRDVLTHHSGIPGDLMNGA
                     FGIKYDKSFLDTLLAWLANDSATYPPGYRWAYSNTAVALLERVIENASGRPFAEYSQD
                     FLDSLGMAPASYFRDNPDLLNRLSRAYIGDVDIGMLQLNVPSSGSIAASANQMGHYLR
                     MLLNEGTLDGRVIVQPETFRAMIQVQNADNALDLTRKMGLSFVLDDADLAWSGPLLWH
                     NGETTAFTSHMEALRNHDIAVFVSVNTKDENNVAGDIAHAALESALAIKRGLARPAQH
                     VSDVSLKSISLPLAFLENFSGSYAGDDLGQVQILRPSNGGLSWDQLEIGSAGQIERNN
                     QGTLTLWSDGFFRTSA"
     assembly_gap    1758..1857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2095..>3691
                     /locus_tag="PK105_06015"
     CDS             2095..>3691
                     /locus_tag="PK105_06015"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU61301.1"
                     /translation="MMFERTVSGYRTQLRILVVLMLFALVVASAFGVSFSNLQIEAIA
                     QEGSAAVQSIMEKKHATAVTVILADANGIIWQGAYGEMEHSKGIPPTKDTLFGICSVS
                     KTFTAVAVMKLVDRGMVSLDEPVVTYIPDFKMEDPRYRDITVRMLLNHSSGLPGQSIL
                     GGMTLSKPYPDYAEGVIRAQWSQHLKFEPGTNAVYTNDGFSILEVLVKRVSGLRFVDF
                     VQREIFDVLGMNSSRFLVSQLPENSYAVPNPADDFSTDAYLNMYGTGGIFSSAADMAK
                     FATSFLTPGRLLSAESIKEMGEDQTKNLFYPIGRDDGFRYGLGWDSYSQPGMAAVGID
                     AWQKGGDLGFYGATLVVLPKEGLTATVLGASNFSSSDAGAIAERVLLKALVEEGRLKA
                     MPKPSPLSAARAALPDFRERAMIAGYYAGMPYSLTRAVFDDEGRLTIEAKQGESWVPS
                     FAMLEKRVDGWYSNDDSPVSVKFVPHEGKVFYVIRFPSGYEHYVTSAPLAQKLDSGPV
                     LPEAWKNRCDSEWLLASDIVDAED"
     assembly_gap    3692..3791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3792..4725
                     /locus_tag="PK105_06020"
     CDS             <3792..4725
                     /locus_tag="PK105_06020"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOU61302.1"
                     /translation="QLMEGMAETSGLSLAEHKLLDASFYLLPDFVIEAAQARAACSGI
                     AVSSPRTADGKLYFARNWDMTQDAMRPYLRYMALVAFNPTKSEVPTGSGSGASGPSLA
                     FANVRPIGQVYVETGINERGIFVELNNGSASDPNTNPEARFSVASLFEFLRTSETFDQ
                     MVQNIVNTPMDASYIIQVASAERAVSVEKPTFDARVIEQHDGALYALNNFARPTYEPW
                     KGKIVELPDNAFDDRQVCLDRMFASQEWQKGVDLAMVKAMMDKTIQQGGPAVEGDLFG
                     TVLQVIVIPEDMRLLFRGYGYSDWADIDLLEIFG"
     gene            complement(4923..5432)
                     /locus_tag="PK105_06025"
     CDS             complement(4923..5432)
                     /locus_tag="PK105_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61303.1"
                     /translation="MNKQLLSLIAIGLLSTFACFAQNPLPLYGNSAAKADTQLTIESM
                     LSYAIQDEYVAEAEYEAIMEKFGYMAPYSNIKEAEGQHIAWLKLLFTSYSLKVPENNA
                     SSYIYVPTTLKEAAETGIRAEIDNIAMYERFLAQPILADPRYADIVAVFVRLRDASKN
                     HLEAFRRQA"
     gene            complement(5792..6598)
                     /locus_tag="PK105_06030"
     CDS             complement(5792..6598)
                     /locus_tag="PK105_06030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU61304.1"
                     /translation="MKEYYENKVAVVTGGASGIGLALCELLLSFGAKAVVLADINADK
                     LKEESARLENEYPGKVLGIQTDVTKQEKVYEMIQKAAKFGSGQIHFLFNNAGLALFKA
                     FDDTTDADWKFAFDINFFGALYGIRAVLPIMRAQGGGHIANTASGAAFSPMAYQSMYT
                     ATKSALVGLTGSLRYEFWDENIRFSTVIPGTVATPIYDKVGGPPESAITPDESARGIL
                     KGLAANERVIIVTEPDRNGAINAFHPETAKYTDEILLDIARQRRRGNIGF"
     gene            complement(6689..7165)
                     /locus_tag="PK105_06035"
     CDS             complement(6689..7165)
                     /locus_tag="PK105_06035"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOU61305.1"
                     /translation="MVWENKTTPILTGNMMGIPKIYADIEDLHILADAYRTRLSFEGS
                     TFLELEMTQLKPLDMEQVNAMNTDINTFGWRYIPKVGEPGADLSQPILFPMHNEAKAA
                     CLGSGKVKWTESTWDQNPMQFYIINALAELPIIEMKPAILMQGREILTEARGRVLK"
     gene            complement(7132..8343)
                     /locus_tag="PK105_06040"
     CDS             complement(7132..8343)
                     /locus_tag="PK105_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61306.1"
                     /translation="MISREAKLPFEVVMLIIAAVVLLVTGVMLFLIYNGILPYYENGL
                     YGLLLIIFALNTITLGRTPFGDIPRSKLLLVLGLLVAVIGMVTCLIPISIRFPRVLLF
                     FCLGPGGLLLFLGMLLAKDKFRSWVKYGGIFRHLMLACSAVYILSIAIALLIWKQDLL
                     TTPMTAVVILFFGAAVFYLAIVLGEIDRTYPEAARLPIGDIKLSIEQTMIIFTSVFMI
                     IIGLVLIPVNLGMLPFSSSAQLGILMVIFAIQMLAFGNTPIGPFPRTWLMIGLGILAV
                     VLGAISSIIPGILVSLLTILIGLLNILGGIIALTKILVQGHRQEAVSRDPSPPILRRL
                     FITQLSLNLLQILFGTSMLIPGLVPGMIIGVILAANGAVLLYLVSILSSTIDWKDRFP
                     LWFGKTKPRLF"
     gene            complement(8803..9540)
                     /locus_tag="PK105_06045"
     CDS             complement(8803..9540)
                     /locus_tag="PK105_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOU61307.1"
                     /translation="MERRFHQVIWAAAIMAVALVVMAVIVVNGFTKVRAAQDTISVTG
                     SAKEPVESDISVWNFSVNIRGNSLQAVYADLKTAIPKIDAFLATQGIPAEEIVASAIN
                     AGSNIYFDEDQQGRRIQHVTFFANRSYKVTSTDIDRLERVSRSAGDLIAQGIALDSVS
                     LEYMYTKLADLRTNLLERATEDAKKRAEAIASSSGAKIGAIKSARMGVFQITPRYSTM
                     VDDYGINDTSSRLKDVTAVVGAEFRLK"
BASE COUNT         2517 a         2215 c         2308 g         2603 t
ORIGIN      
        1 gatgagatct aggcctggta ccatctccac aacgagttgt gatgcatttt cattgtttgt
       61 agtatcgcgc gccaaattac cataggaata atcgatacca gcatggaaag ggccggtggg
      121 taggccaaga ttctctttat tgcacctatc gtttgcagct ttgaaatgca cgcctttaga
      181 tgggattttc acatggtttt gatttgcagt gatcctatag cggatctcct ttttcagctt
      241 tactcaatga ccattgtgat ttacacttat aatgactttt cttaaaaatg ttgtaaattc
      301 tcgctcattt tcatcagatc aaaaagaaga ttagtggcct gttctctgag ggggaggtat
      361 atgtttatta agatagggcg tttgatcgca gtcctatgca tatggggttt tgcgctctcc
      421 gcgctgcctg cgcaaaatat attggatccc tcgtggagtg gtgtagcaga tgaaattcgc
      481 ccaaaaatcc aacagaggat gagcgaatct gatataaaag ggctcatgat cgtgcttgtg
      541 gatgaagaga aaactgtgtg gagcgaaggt tttggatttt ccgatatcag cacgaacaag
      601 aaagtagacg cggatacgat gttcgaaatt ggttctgttt ccaagacttt cagtgggctc
      661 atggttatgc aactctatga acaaggcaaa ctcgacattg ataagccgct ggtcacctat
      721 attccggaat tcaagctcat gccgcctgtg ggcaattttc ctcgcgtcga taggccgata
      781 acggtgcgag acgtactcac gcaccactca ggaattcctg gtgatttaat gaatggcgct
      841 ttcggtatca aatacgataa gagcttcctc gacaccttgc tcgcatggct tgcaaatgac
      901 tcggcgacct atccgccagg gtatcgatgg gcatattcga acaccgccgt ggcactcctg
      961 gagcgcgtta tagaaaacgc ctctggcagg ccgttcgcgg aatactcgca ggattttctt
     1021 gattccttgg ggatggctcc tgcatcctat ttcagagata atcccgacct tctaaatcga
     1081 ctttcaagag cctatattgg agacgttgat attgggatgt tgcagctcaa tgttccttcc
     1141 tctggctcta tcgcagcaag tgcaaaccaa atggggcact accttcgaat gctgctaaac
     1201 gaagggacgc tggacggcag agttattgtt caaccagaga cctttagggc gatgatacaa
     1261 gtccaaaatg ccgataatgc actcgatttg acgcgtaaaa tggggctttc tttcgtgctc
     1321 gatgatgctg accttgcatg gtcgggtccg ctgctatggc acaatggaga aacgacagca
     1381 ttcacctcgc acatggaagc gttgaggaac cacgacattg ctgttttcgt tagtgtcaat
     1441 acaaaggacg aaaataatgt tgctggagac atcgcacatg cggcgttaga gagtgcgctc
     1501 gccataaagc gtgggctcgc tcggcctgcg cagcacgtct cagacgtttc actcaagtca
     1561 atttcacttc cgcttgcgtt tctggaaaat ttctctggca gttacgcggg agacgattta
     1621 ggtcaggttc agattttgcg accatcgaac gggggccttt cttgggacca gctcgaaatt
     1681 ggatcagcag gccaaattga gcggaataat caaggcacac taacactctg gtcggatggg
     1741 ttctttcgaa catcggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct
     1861 gtaagcgatg aggtcggtta tatacgcgga ttggggcgga ttggggggag cgcacttcgc
     1921 tttcaaaacc gcgatgggga acaagaacta cgtttcatga tggatgtgtt tcgttttggt
     1981 gctaaataaa atagatgtgg tgagtagatg cttgtgataa agcttcgtaa aagaggataa
     2041 aaagctaaga agcgacacgg tgagtttatg aataagctca gcgaggagaa gaacatgatg
     2101 tttgaaagaa cagtctctgg atatcgtacg cagctgagga ttttggttgt ccttatgcta
     2161 ttcgcgctag tcgtggcatc tgcgtttggt gtttcgttca gtaatctaca aatcgaagcc
     2221 atcgcgcagg agggatctgc ggcggttcaa tcgataatgg aaaaaaaaca tgcaactgct
     2281 gttaccgtga tacttgcaga tgcgaacggc attatctggc aaggggcata cggagaaatg
     2341 gaacacagta aaggtattcc gccgacaaaa gacacgcttt tcggaatctg ctcagtctca
     2401 aagaccttta cggctgtggc tgtgatgaaa cttgtggatc gtggaatggt gtctttggac
     2461 gagcctgtag tgacctacat tccagatttc aaaatggagg atcctcgtta ccgcgatatc
     2521 accgtccgca tgttgctcaa tcatagctca ggcctccctg ggcaatcgat tctcggggga
     2581 atgacgctct ccaaacccta tcccgattat gctgaaggcg tgattcgcgc ccaatggtcg
     2641 cagcacctca aatttgaacc ggggaccaat gctgtctaca caaatgatgg tttttcgatt
     2701 cttgaagtgc tcgtcaagcg cgtaagcggg ctgagatttg tcgattttgt gcaacgcgaa
     2761 atttttgatg tcctcggtat gaattcatcg cgctttcttg tttcgcagct tccagagaat
     2821 tcttacgcag ttccgaatcc ggcggatgat ttttctactg atgcgtatct caatatgtat
     2881 ggcaccggag gtattttctc ttctgccgct gacatggcca aatttgccac atctttttta
     2941 accccgggac gcctgctttc cgcagaatct atcaaggaaa tgggagagga tcagacaaag
     3001 aatctgtttt atcctattgg gcgggatgac ggatttcgct atggccttgg gtgggactcg
     3061 tacagccagc caggcatggc tgcggtaggc atcgatgcat ggcagaaagg cggagacttg
     3121 ggtttttatg gggcaacgct tgtggtgctc cctaaggaag gccttactgc cacggtcctt
     3181 ggcgcgagca atttctcgtc ttcagatgca ggagctattg cggaaagagt gcttcttaag
     3241 gctcttgtag aagaaggaag gctcaaagca atgcccaagc catcgccttt gagcgccgca
     3301 agggcagcac tgcctgattt tagagagcgc gcgatgatcg cgggatacta cgctggaatg
     3361 ccctattcgc ttacccgcgc ggtgttcgac gatgaaggac gcttgaccat cgaagcaaag
     3421 caaggcgaat cgtgggtccc ttcgttcgca atgcttgaaa agcgcgtaga tgggtggtat
     3481 tcaaatgatg attcgccagt gtctgtaaaa ttcgtgcctc acgaaggcaa ggtattctat
     3541 gtaatccgct tcccatcagg atacgagcat tatgtgacct ccgcgccact tgcccaaaag
     3601 ttggattccg gtccagttct gccagaagcc tggaaaaatc gatgcgactc agagtggctt
     3661 ctggctagcg atattgttga tgccgaagat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nacagctcat ggagggtatg gcagaaacct cgggcttgtc attggcggag
     3841 cacaaattgc tcgacgcatc attctatttg ctacccgatt ttgtcattga agcggcacaa
     3901 gcccgcgcgg cctgttcggg cattgcggtt tccagtccca gaaccgccga tggcaagctc
     3961 tactttgccc gcaactggga catgactcag gatgcaatgc ggccttatct ccgttacatg
     4021 gcactcgttg cctttaatcc gacgaagtca gaagtaccta caggatcggg gagcggcgca
     4081 tctggtcctt cgctcgcgtt tgcgaacgta aggccgatag gccaagtgta tgtggaaacg
     4141 ggtatcaacg agcgcggtat ttttgtcgaa ctcaacaacg gttcggcttc tgacccgaac
     4201 acaaaccccg aagcgcgctt ctcagtggca tcgctttttg agttcttaag gacgagcgaa
     4261 accttcgacc agatggtgca aaatatcgtg aacacgccga tggatgcctc gtatatcatc
     4321 caagtggcaa gtgcggagcg cgcagtttcg gtggaaaagc ccacttttga cgctcgagtg
     4381 atcgagcagc atgatggcgc gctctatgcg ctcaataatt ttgctcgacc aacctacgaa
     4441 ccatggaagg gaaagatcgt cgagctgccg gacaatgctt tcgacgacag gcaagtttgc
     4501 cttgatagaa tgttcgcatc gcaagaatgg caaaaggggg tagatctcgc tatggtcaaa
     4561 gcgatgatgg acaaaaccat tcagcaaggc ggaccagcgg tggaaggtga tctctttgga
     4621 acagtgctgc aagtcattgt aatacctgaa gacatgagac tgcttttccg tggctatggc
     4681 tattcggact gggcagacat cgatttactt gaaatctttg gatgataatt tgaaaagcaa
     4741 tatagcaaag cgatgaatgc cgagaggcga tcttgcgctc gaacgcttgc atgcgctcgg
     4801 gcgcgatctc ttcgccagct tcatttcgat ccgctcttgc ttagggctca gccttccatc
     4861 gccctataca gctcaagggg atttattccc tcaagcccaa gtactcgccg taggtttgtt
     4921 gcttatgctt gccttctgaa tgcttcaaga tgattttttg aagcatctct caatctaaca
     4981 aacactgcaa caatgtcagc atagcgcgga tcggcaagaa tgggctgagc aagaaatctc
     5041 tcatacatcg caatgttatc tatctcagca cgaattccag tttccgcagc ctctttcagt
     5101 gtcgtgggaa cgtaaatgta actgctggcg ttgttctccg ggacttttag ggaataggaa
     5161 gtgaaaagca gcttgagcca tgcgatatgt tgtccctctg cttctttaat atttgagtac
     5221 ggagccatat aaccaaattt ttccatgata gcttcatatt ctgcttcagc cacgtattca
     5281 tcttgaatag cataggaaag catggattct atagtaagct gcgtgtcagc ttttgcggct
     5341 gaattgccgt acagcggtag agggttctgt gcaaaacaag cgaaagtact aagaagtcca
     5401 atagcaatta atgaaagcag ttgcttattc atctttgatc tccttgcggt ttgtctactt
     5461 tatagtcgcc taaagtgcga ttgtggttgc aaaaaccgcc gcggatagtg caagatttag
     5521 ttcgcaattt ggagcagata ggtgatgcta catatggcca gattaaaggg cttagaacat
     5581 cagcgtatgg atgctttggc cgggtcatgg ttttgtattc tataaagctc atgattcttg
     5641 tcattatgga tattataaag ctgaagaatg ttgagggaaa gtgaatcagg cttaagagcg
     5701 cgactatata ttttgccttg ttcaatgcta ttgcgcgatc gcgatctatc tacctctttt
     5761 ttttactgac ttcgcattct gagaaagtcg ctcaaaagcc aatattgccg cgccgtcttt
     5821 gacgtgcgat gtccaatagg atctcatctg tgtactttgc ggtttcggga tgaaaggcat
     5881 taatcgcgcc attgcggtcg ggctcggtaa caatgataac tctttcgttt gctgctaatc
     5941 ccttgagtat tccacgagcg gactcatctg gagttattgc ggattcggga gggcctccga
     6001 ctttatcata tatgggcgtt gcaacggtac caggaatgac agtagaaaaa cgaatgttct
     6061 catcccaaaa ctcatagcgc agcgaaccgg taagccctac caaggcggat ttggtggccg
     6121 tgtacatgct ctgataggcc atcggtgaaa aagccgctcc tgaggcggta tttgctatat
     6181 gtccgcctcc ttgtgcgcgc atgatgggca ataccgctcg gataccatat aaagcaccaa
     6241 aaaaattgat atcaaaggca aacttccaat cggcatctgt tgtatcgtcg aaagccttga
     6301 acaacgcaag accggcatta ttaaaaagga agtgaatttg accgcttcca aatttcgccg
     6361 ccttttgaat catctcatag accttttcct gcttggtaac atcggtttga attcccagga
     6421 ctttcccagg gtattcgttc tcaagcctcg cgctttcttc tttcaattta tcagcattta
     6481 tatctgctag aacgacagcc tttgcgccaa atgataggag caattcgcac agtgccaaac
     6541 ctattccaga agctccaccg gttactacgg caaccttgtt ctcataatat tctttcattc
     6601 caaaatcctc cttttaaaat tatgcagatt ccagagatgg atatctgctt tagatatcgg
     6661 ctgctcaaat tggcatggaa atacgcatct acttaagaac acgtcctcgc gcttcggtga
     6721 gaatctcccg tccttgcatc aatatggcag gcttcatttc gataattggc agttctgcaa
     6781 gagcatttat tatgtagaat tgcatggggt tttggtccca agttgactct gtccatttga
     6841 ctttcccact acccaagcag gctgctttcg cttcgttgtg cattggaaaa agaataggct
     6901 gactcaaatc tgccccaggc tcgcctactt tcggaatgta gcgccatcca aatgtattta
     6961 tgtcagtatt catggcattt acctgctcca tatccaatgg tttaagctgg gtcatctcaa
     7021 gttccaaaaa ggtgctgcct tcgaaactca ggcgagtacg gtatgcatcc gcaaggatgt
     7081 gcaggtcttc aatgtcagcg tagatcttcg ggatacccat catgtttcct gttagaatag
     7141 gcgtggtttt gttttcccaa accacaaggg aaaacgatcc ttccaatcga tcgtgctgga
     7201 caaaatactc acaagatata gaagaacagc cccattcgca gcaagaatca cgccgatgat
     7261 cattcccggt accaagccag ggatgagcat cgatgtaccg aataggattt gaaggagatt
     7321 caggcttagc tgtgttatga agagtcttct taatatcggc ggactgggat cgcgtgatac
     7381 ggcctcttgt ctatgacctt ggactaagat tttagtcaga gcaataattc cgcctaagat
     7441 gtttagcaaa ccaatgagaa ttgtcagcag cgatacaagg atcccgggaa ttatgcttga
     7501 gatggcccct aacactacgg ccaaaatccc aagtccaatc atgagccatg tacgaggaaa
     7561 cggaccaatc ggcgtattcc cgaatgcaag catctgaata gcaaagatta ccattaggat
     7621 tcccaattgt gcactgcttg aaaaaggaag catgcccaaa ttcactggga tcagcaccaa
     7681 accgatgatt atcatgaaaa cactcgtgaa tataatcatc gtctgctcga tggaaagctt
     7741 gatgtcgcca ataggaagtc tcgctgcctc tgggtatgtc cgatcaatct ctccgagcac
     7801 gatggcaaga tagaatactg ctgcgccaaa aaatagaatc accacagctg tcattggcgt
     7861 cgtcaataga tcctgcttcc aaataagcag cgcgatggct atggataaaa tatagaccgc
     7921 gctgcatgca agcatcagat gtcgaaaaat tccaccatat ttcacccaac tcctaaattt
     7981 gtctttagcc aacaacatcc ccaaaaagag aagcaagccg cccggaccaa gacagaaaaa
     8041 caacagcaca cggggaaagc gaatcgaaat aggaataaga caggttacca taccaattac
     8101 cgcaaccaac aatcctaaga ctaataggag ctttgacctg ggtatgtcac caaatggggt
     8161 tcttccaagg gttatcgtgt ttaaggcgaa tatgattaga agcaatccat ataaaccgtt
     8221 ttcatagtag ggaagaattc cgttatagat caagaagagc ataaccccag taactagtaa
     8281 gactaccgca gcgataatca gcataacgac ttcgaaaggg agctttgcct ctctggaaat
     8341 catacgcacc gtccttctta ccaggtctcg aatatattag ctcgaatgca gttgcttttc
     8401 ttcgaattca gcaaaactcg gtttacaggt gatgtgtagc atcgcgtttc ctttctgaga
     8461 catggaccgt cgcagatacg ccatctggct gagcgatttg ttgatttgct atcaaaccta
     8521 tattcatttg ccaactataa atctgggatt atcaattgta aagctatctt gcattgacta
     8581 atcaaagatc tgtccaaaaa ggaagcatta actcataaca gattctaagc cttacaaaga
     8641 aagccgtacg ctcggtcttg ctaagagatt gatattcgct gtttcttcta ttaattttgt
     8701 cgccttgata attagcccgc tcatcaaaat ccacatagaa gaatggagcg cctcatagct
     8761 tttgtattat atgagatgcc tactaatgaa tgccatttag cgtcatttaa gcctgaattc
     8821 agcgccgaca actgcggtga catcttttag acggcttgaa gtatcgttaa tcccatagtc
     8881 atccaccatg gtcgagtagc gtggtgtgat ctggaaaacg cccatgcgcg cgcttttgat
     8941 tgctccaatc ttggcgcctg atgaagaagc aatagcttca gctcgctttt ttgcgtcctc
     9001 tgtggcgcgc tccagcagat tagtccgtaa atcagctaac ttggtataca tatattcgag
     9061 agagacagag tctagcgcaa tgccctgggc gataaggtcg cctgcactgc gcgatacgcg
     9121 ctccaatctg tctatatctg tcgatgtcac tttgtaactg cggttggcaa agaaggtaac
     9181 atgctgtatg cgacggcctt gctgatcttc gtcaaaatag atattgctgc cagcgtttat
     9241 ggcactggcg acaatttctt cggctggtat gccttgtgtg gccaaaaatg catcgatctt
     9301 ggggatggct gtctttaaat ctgcatatac agcctgaagg ctattcccgc ggatgttgac
     9361 ggaaaagttc caaacggaaa tgtccgattc gactggctct tttgcgcttc ctgttacgct
     9421 gattgtgtct tgtgcagcgc gaaccttcgt aaaaccattg actacaatga cagccattac
     9481 aactaaagcg actgccataa tagctgcagc ccaaattact tgatggaatc ttctctccat
     9541 aatcttctac ccccacttag agcaaaacct agcagtaatc ccaggtgtta ttctattatt
     9601 ggctttagcg cacaactcct gggtgatttc atctctttgc tccagtatat aaggccacgt
     9661 caattttatc cctattctct tggatcattc aatcaggcgg actgaaagat ctcgatattt
     9721 aatgatgcga tgatttctcc agaatcctgc cacgcaatta agtggacttt gcattttaca
     9781 gcagaccttg caactaccaa attgcgaact taattttaca ctataataaa gtcgtgctat
     9841 act
//
LOCUS       DAOOWY010000066         9748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_6960_length_9748_cov_12.103167, whole genome shotgun sequence.
ACCESSION   DAOOWY010000066 DAOOWY010000000
VERSION     DAOOWY010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6960_length_9748_cov_12.103167"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   1..223
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..23
                     /rpt_unit_seq="cacgcgcccgcgtggggcgcgac"
     gene            complement(468..758)
                     /gene="cas2"
                     /locus_tag="PK105_06050"
     CDS             complement(468..758)
                     /gene="cas2"
                     /locus_tag="PK105_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOU61308.1"
                     /translation="MFVLVSYDVSITTPGGKRRLRRVAKTCLNFGQRVQCSVFECVVE
                     PAQWVALKSQLEAIIDKDHDSLRYYYLGSNWKHRVEHIGAKPSLDLDDPLVV"
     gene            complement(762..1793)
                     /gene="cas1c"
                     /locus_tag="PK105_06055"
     CDS             complement(762..1793)
                     /gene="cas1c"
                     /locus_tag="PK105_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HOU61309.1"
                     /translation="MRKLLNTLYVTSQGSYIHKEGETIIVEREQQKVLQLPVHTLGGI
                     VCFGNVLCSPFLLGFCAEHDISISFLTEYGSFLASVRGPVSGNVLLRREQYRIADNSE
                     KSAKIAANIVTGKLMNSRLVMNRAIRDHGDKIDIATIRRASSSIYRLIEELALAVDLD
                     EIRGIEGMAASEYFAVFNQLIVDQKEDFVFNERNRRPPLDPVNALLSFIYTLLVHDVR
                     SALETVGLDPTVGFLHRDRPGRPGLALDLMEEFRPVIADRLVLSLINRRQVAPNGFKK
                     AESGAVVMDDSTRKTVLVEYQNRKQDEIFHPYIEEKIPLGLLFFVQANLMARFIRGDI
                     DGYPPFFWR"
     gene            complement(1815..2477)
                     /gene="cas4"
                     /locus_tag="PK105_06060"
     CDS             complement(1815..2477)
                     /gene="cas4"
                     /locus_tag="PK105_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOU61310.1"
                     /translation="MYSEDDLVPISALQHILFCERQYALIYIEQVWEENRFTAEGKVL
                     HERVDVERHESRRLFRQEYGMAVRSLERGLIGKCDLVEIYLAPGEIVAQAVPIEFKRG
                     RNKEEDVDRVQLCAQAFCLEEMFGITVADGQLYYLQEHRRTTVNLGGELRAKTMELIA
                     RIQQLQSSGITPPAIYQKRKCDRCSLLEVCMPKSAGSGGKEVDRFVRSQVRAARGEPS
                     QS"
     gene            complement(2482..2760)
                     /locus_tag="PK105_06065"
     CDS             complement(2482..2760)
                     /locus_tag="PK105_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61311.1"
                     /translation="MCSDYRHRGLDIGPTLAQRSVFLFGPRQTREFLRLCFHILIDTM
                     LGYELPSYTSTVERKARESRPRKVDGIDILPWREFLGRLWAGDIVAGG"
     gene            complement(2940..3818)
                     /gene="cas7c"
                     /locus_tag="PK105_06070"
     CDS             complement(2940..3818)
                     /gene="cas7c"
                     /locus_tag="PK105_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOU61312.1"
                     /translation="MDLDKRYDFVLFFDVKDGNPNGDPDAGNLPRVDAETGNGLVTDV
                     CLKRKVRNYIGLLKNEQPPYEIYVKEKAILNNQNARAYQALNIDLSKDEGKRKGGDKI
                     DEARAWMCKNFYDVRTFGAVMSTGINCGQVRGPVQLTFARSVDQIVSSEHSITRMAVA
                     TEAEAVKQGGDNRTMGRKFTVPYGLYRAYGFVSAPLAKQTGFSQEDLDLFWEALQNMF
                     ENDRSAARGLMGARRLIIFGHSSLMGNASAQELFDRVKVERATDPTKPARDFSDYRVT
                     LDGQELEEFKTVVSVQ"
     gene            complement(3843..5588)
                     /gene="cas8c"
                     /locus_tag="PK105_06075"
     CDS             complement(3843..5588)
                     /gene="cas8c"
                     /locus_tag="PK105_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOU61313.1"
                     /translation="MILQALKAYYDRMALDPESAIAPEGWEWKEIPFIVVLGNNGDLL
                     QIEDTREGEGKKKRGKLFLVPQSVKKTSGIAANLLWDTANYVFGIVNTEGMDNEQTSK
                     ALIRASEQKQAFVKRITEELPQTPNLKAIITFLTTVAKDRLESENAWQDIYASNSVVS
                     FRFDEEKELYCQSKEVQEVLEQKLSVKEKKDICLVSGEKDKIAELHIPIKGVYGAQSS
                     GANIVSFNLEPFRSFNKKQGENSPVGESSMFAYTTALNTLLNRQSSQHLLIGDASTVF
                     WSEKRTHFESDFASFFKEPDKDNPEAGIQKIRNLFEAPLTGGYLDDDSDTKFYVLGLA
                     PNAARIAVRFWEVGTISDFAEKIRQHFEDLNIVKPSSEPPYYSIWRILVNIAIQDKSE
                     NIPPNIAGDFMRSILDGTPYPATLFQAVLRRIHSDTENRVKPVRAALIKAYLNRYLRA
                     HPNQKEKEVEMGLDTEQPSIGYQLGRLFAVLEKIQEEANPGLNATIRERYYGAASSSP
                     VTVFGTLMRLKNHHLAKIDNKGRVVNLERLIGEIVGHIGDFPSHLDLHEQGKFALGYY
                     HQRQDLFTKKENDNQ"
     gene            complement(5585..6316)
                     /gene="cas5c"
                     /locus_tag="PK105_06080"
     CDS             complement(5585..6316)
                     /gene="cas5c"
                     /locus_tag="PK105_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOU61314.1"
                     /translation="MKDWCLEVWGEYACFTRPEMKVERVSYDVMTPSAARAIFEAILW
                     KPAIKWHIRQIEVLRPIRWISVRRNEVGKIENSPTQAHMEGRSSDPMGLYIEDCRQQR
                     AGLFLRDVRYRIHAWFEFIPPDKRMYNHPSAEEVWADEEEREQVSRADENEAKYAAMF
                     ERRARKGQCFHRPYLGCREFACDFRLIEGGTEKQPPIDKTDELGWMLYDMDYRDPSDP
                     KPTFFRAKIENGIMNTDQRIVEVRG"
     gene            complement(6329..8566)
                     /gene="cas3"
                     /locus_tag="PK105_06085"
     CDS             complement(6329..8566)
                     /gene="cas3"
                     /locus_tag="PK105_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOU61315.1"
                     /translation="MNNPELIAHVRKNKDGTWANPQPLIDHLEGSAKLASRFAEAFNS
                     ASWAYAAGFTHDIGKAASIWQHYLRVKSGYDDEAHLEIKAGKQDHSSPSAKFVEEQFG
                     KATGRILSYCIAGHHSGLPDWIGSPSSLSFRLQNIKTCSIPEEYRKDPRFSGSFVPPW
                     KFDNRSLDMSLWIRMIFSCIVDADFLDTESYMEPDNKKTRGDYLSLAELLIQFNSYMD
                     KMIKKTIEEENTIVNQARQKILVECRSAAKSKAGFFSLTVPTGGGKTLSSMAFALEHA
                     AKFGKERIIYVIPYTSIIEQNADVFRKVLGEEQVIEHHSNLDENDSSPKSRLAAENWD
                     APIIVTTSVQFFESLFAAKPSRCRKLHNIVNSVVVLDEAQLIPTGYLEPILATMKLLC
                     EHYKVTFVLCTATQPAFEAQDDYPQFPGLPRGSVREIIQDVPRLYRELRRVEVDPVDT
                     NTTRRWEEIAEELKSFERVLCVVSDRRSCRELHALMPKGTYHLSALMCAQHRSDCIAE
                     IKERLKTTEPVRVISTQLVEAGVDIDFPVVYRAMAGLDSIAQSAGRCNREGKLKTNGG
                     LGKVVVFTPQRKPPAGILRKASETTARLLDSGLKDPIDHAAFSQYFSELYWKVNSLDE
                     KGIMKMLSPDMQDCGIQFRSAAEAFHIIDDAMQKTVLVPYGEGEKFIAELKAKGPERW
                     LLRKLQRYSVTIYNDQFYALLNRGSIEEVSPGLFALTCKIEYDDVVGLLIDEMPNDPK
                     SFIAI"
     gene            complement(8761..9228)
                     /locus_tag="PK105_06090"
     CDS             complement(8761..9228)
                     /locus_tag="PK105_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61316.1"
                     /translation="MNAKVVYLIQFRKDYVELFFERQSGAFDYVDMFSGEDALTKAEN
                     MAFSLIEETPRIDLSFQNCFGKLLIYQSDISHPTIRQLCPCTTPEEDILAFFIRYCSF
                     CKAIYIWAELFDFAKKSINQMDIAAYLNGLARQSAILEGKFFFLGSDSLKLSI"
     gene            complement(9347..>9748)
                     /locus_tag="PK105_06095"
     CDS             complement(9347..>9748)
                     /locus_tag="PK105_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOU61317.1"
                     /translation="MELRTFSISRMDNVRAAGAPCFAIPSDDAIESYISSSYGIFKGE
                     VLGIATLRFFGGAARAIRIQQWHKDQIIDPVPDATDPDAIDLSLPVHNWTEILGRALR
                     CGSNCEVLGPPEFRKLWEEEIKRMVEKVGEK"
BASE COUNT         2523 a         2379 c         2042 g         2804 t
ORIGIN      
        1 cacgcgcccg cgtggggcgc gaccacttgc gcgtgacaaa gacaagacca tcgcgctgtt
       61 tcaatccacg cgcccgcgtg gggcgcgaca caccacgctt gagggtggca acgtgccgcc
      121 tgaggtttca atccacgcgc ccgcgtgggg cgcgacgcgc attcaacggg ggttcttgag
      181 gcggttggcg cgtttcaatc cacgcgcccg cgtggggcgc gactatatgc atttcaatta
      241 tatgcactac ataaaaataa ttcaatagaa ttgcgaaaat tcaaaatata gtcggaaaac
      301 tcttaaaaat tccttccctt gattttcaaa gatcgatata ataaagaatt agatataacg
      361 cgaaaaagcc atatttttct gtgtctttac gtttcgcgtg gttttgcact ctcgctggca
      421 caagggcaga aggtagcaca gcattaaaaa catactatat actcgttcta aacgaccaaa
      481 ggatcatcaa gatccagact cggttttgct ccaatatgct ccacccggtg cttccaattc
      541 gatccgagat aataatatcg caaactatca tgatctttat ctattatagc ctcaagctgg
      601 ctttttagag ccacccattg cgcgggctct acaacacatt cgaaaacact acattgtact
      661 ctttgaccaa aattgaggca agttttagct actcggcgca atctcctttt tcctccagga
      721 gttgtaatcg atacatcata actcactagg acaaacatct atcacctcca gaagaatggt
      781 ggatagccgt ctatatctcc gcggatgaat cgtgccataa ggtttgcttg tacaaaaaac
      841 aagagcccaa gcggaatttt ctcttcaatg tatgggtgaa aaatctcatc ttgttttcta
      901 ttttggtatt caacaagcac ggtttttctt gtcgaatcat ccattacgac cgcaccgctc
      961 tctgcttttt tgaaaccatt tggagcaact tgtcttctat ttattaaaga gagcacaaga
     1021 cgatcggcaa taactggacg aaattcttcc atgaggtcaa gagcaagacc aggccttccg
     1081 gggcgatctc gatgaagaaa tccaactgtg ggatcaagcc caacggtttc tagtgctgat
     1141 cgcacatcat gaacaagcag cgtatatata aaagataaaa gagcattcac aggatcgaga
     1201 ggaggccttc tattcctctc attgaaaaca aaatcctcct tctgatcaac aattagctga
     1261 ttgaaaactg caaaatactc ggaagcagcc ataccttcaa tcccgcgtat ttcatctaaa
     1321 tcgacagcaa gagctaactc ttcgataagc ctatatatgg aagaagaagc tctccttatc
     1381 gtagcaatat cgatcttatc accatgatcg cgaatagcgc gattcatgac cagtcgcgaa
     1441 ttcataagct tgcctgttac aatatttgca gcaattttcg ctgacttctc tgagttatct
     1501 gctatgcgat attgctctcg acgaagaagg acattgccag acactggccc tcgtaccgaa
     1561 gcaagaaaac taccatactc tgtaagaaac gatatactta tgtcatgttc tgcacagaat
     1621 ccaagtagaa aaggagaaca aagtacattg ccgaagcata cgattccgcc cagtgtgtgg
     1681 acaggcaact gaaggacctt ttgctgttct cgctctacta tgatggtttc accttctttg
     1741 tgaatatatg aaccctggga agtgacatat aatgtgttga gcagttttct cactcttgcc
     1801 tccttttttt gatattatga ttgactgggc tctccccttg ctgcgcgcac ctgtgagcgt
     1861 acaaagcgat cgacctcctt ccctcctgat ccagcgctct tgggcataca tacttccaaa
     1921 agtgagcatc gatcgcattt ccgcttttga tatatagctg gaggggttat cccagaagat
     1981 tgcaattgct gtattctcgc tatgagctcc atagtctttg ctctcaattc gccaccgaga
     2041 ttcacggttg ttctacgatg ctcctgcaaa taatacagct gaccatctgc taccgtgatc
     2101 ccaaacattt cttctaggca aaaagcttgc gcgcagagct gaacgcggtc cacatcctcc
     2161 tctttgtttc gccctctttt gaactcgatt ggcactgcct gagcaactat ttctcctgga
     2221 gcaaggtaaa tctcaacaag atcgcacttt cctattaagc cacgctctaa cgaacgcaca
     2281 gccataccat attcctgacg gaaaagcctt cgcgattcgt gacgctccac atcaactcgt
     2341 tcgtggagga cctttccttc ggcggtgaaa cggttctcct cccagacctg ctcgatgtaa
     2401 atgagggcat actgacgctc gcagaagagt atgtgctgga gcgcagagat cggaacaaga
     2461 tcatcctcag agtacaagag atcaacctcc tgctacaatg tcgcccgccc agagccggcc
     2521 aaggaattcg cgccatggca gaatatcgat tccgtcgacc ttccggggac gggattccct
     2581 tgcctttctc tccactgtag aggtatagga cggaagttca taaccaagca ttgtatcgat
     2641 gagaatatgg aagcaaagac gtaagaattc cctcgtctgc cgcgggccaa agaggaagac
     2701 cgacctctgt gcaagagtag ggcctatatc caagcccctg tggcgataat cgctacacat
     2761 ccatcactct ccaaagtcat aataatcgcc aatatgattg gcgattatta tgataaacga
     2821 caacagaatt ggcgcttatg ggttgctgct tatgattgca tagaaagctt tctcccgcac
     2881 tccatcaacc ccaccgcaac cgaaacacta actgtaccag cgattgatat ctcctcctat
     2941 cattgcactg acactacagt cttaaactcc tccagttctt gcccgtccag agtcaccctg
     3001 tagtctgaga aatccctggc cggctttgtc gggtcagtcg cgcgctccac cttcacacga
     3061 tcgaagagtt cctgggcgct cgcgttgccc atgagcgagg agtgtccgaa gataatgagt
     3121 ctgcgtgctc ccataagccc ccgcgcggca gaacggtcgt tctcgaacat attctggagt
     3181 gcttcccaga aaagatcgag gtcttcttga gaaaaacctg tttgcttcgc cagaggtgct
     3241 gagacaaagc cataggcgcg atagagccca tatggaactg tgaacttacg acccatggtg
     3301 cgattgtcgc caccctgctt caccgcctct gcttcagtcg ccacggccat acgcgttata
     3361 ctatgttctg atgaaacaat ctgatcgaca gagcgagcga atgtcaattg cacgggccct
     3421 ctgacttgtc cgcaatttat acctgtggac atgactgcgc cgaaagtcct cacatcatag
     3481 aaattcttgc acatccaagc acgtgcttcg tctattttat cgccaccttt ccttttccct
     3541 tcatccttgc tgagatcgat attaagagcc tgataggcac gggcgttttg gttgtttaaa
     3601 atagcctttt cttttacata gatttcgtaa ggcggttgct catttttgag aaggccaatg
     3661 taattcctta cttttcgttt gaggcaaaca tcagtaacaa gtccattgcc tgtttctgca
     3721 tccacgcgtg gaaggttacc agcatcggga tcaccattag ggttgccatc tttgacatcg
     3781 aagaaaagta caaaatcgta gcgtttgtcc aagtccataa taatctcctt tccttgtgtt
     3841 ggttattgat tatcgttctc tttcttggtg aaaagatctt gccgctgatg ataatatccg
     3901 agggcaaatt ttccttgctc atgaagatcg aggtgtgatg ggaaatcacc gatatgaccg
     3961 acgatctcac cgattaatcg ttcgagattt acgacgcggc ctttattgtc tatttttgca
     4021 agatgatggt ttttgagacg cataagcgtt ccaaaaaccg tcacaggcga gctgctggcc
     4081 gcaccataat agcgttctct gattgttgcg ttcaggcctg gattggcttc ctcttgaatt
     4141 ttttcaagaa ccgcaaaaag ccgtccgagt tgatagccaa ttgagggctg ttctgtgtcg
     4201 agtcccattt ctacctcctt ttctttttgg ttcggatgag cacgcagata tctattaaga
     4261 taagccttta ttaaggctgc tcgtactggt tttactctgt tctcagtatc gctatgaatg
     4321 cggcgcaaaa cggcctgaaa aagtgttgca ggataaggcg tgccgtccaa gattgagcgc
     4381 ataaagtccc ctgcgatatt gggaggaata ttctcgcttt tgtcctgaat ggcgatgttc
     4441 acaagaattc gccatataga atagtatgga ggttcagaag atggttttac gatgttcagg
     4501 tcttcaaaat gttggcggat cttctccgca aaatcagaaa tggtacctac ctcccaaaac
     4561 cttaccgcaa ttcgcgccgc attaggcgca aggccgagaa cgtagaactt tgtatcgctg
     4621 tcgtcgtcaa ggtaacctcc tgtcagcggt gcttcaaaga gatttctaat tttctggatt
     4681 cctgcctcgg gattgtcttt atcgggttct ttgaaaaaag aggcaaaatc tgattcgaag
     4741 tgggtccttt tttctgacca gaaaacagta gaggcatcgc caatcagaag atgctgtgat
     4801 gattgtctgt ttagtagtgt attaagtgca gtagtatagg cgaacattga actttctcca
     4861 accggagaat tttccccttg ttttttattg aaagatcgaa aaggctccaa attaaatgag
     4921 acaatattcg ctcctgaact ctgtgcacca tatactcctt tgatggggat atgcaattcc
     4981 gcaattttat ctttctcccc agatacaagg cagatatctt ttttttcttt tacagacagc
     5041 ttttgctcca agacctcctg tacctctttt gattggcaat acagttcttt ttcttcatca
     5101 aagcggaaac ttacaacaga attagaggca taaatatctt gccatgcatt ttcgctttca
     5161 agtctatctt ttgcaaccgt tgtaagaaat gtgatgatgg cttttagatt gggagtctgt
     5221 ggaagttctt ctgttatacg tttgacaaaa gcttgtttct gctcagatgc ccgaattaag
     5281 gctttagaag tttgttcatt atccatgcct tcagtgttca caataccaaa cacgtaattt
     5341 gctgtgtccc atagaagatt tgccgcaata ccagaagtct tttttacgct ctgtggaacc
     5401 aggaacaact ttccacgctt tttcttccct tcaccttctc tcgtatcctc aatctgcaga
     5461 agatctccat tgttgccgag gacaacaatg aatgggattt ccttccattc ccatccctcc
     5521 ggcgctattg cgctttcggg atcaagtgcc atacgatcat agtaagcttt tagagcctgg
     5581 agtatcatcc ccgtacctcc actatgcgtt gatccgtgtt cataatgcca ttttcaatct
     5641 tcgcacggaa aaaggtcggc ttcggatcac taggatccct gtaatccatg tcgtagagca
     5701 tccacccgag ttcatcggtc ttatcgatcg gtggctgttt ttcagttccg ccctcaataa
     5761 ggcggaagtc gcaggcgaat tctctgcaac caaggtacgg gcgatggaaa cattggcctt
     5821 ttcgagcccg ccgctcaaac atagcggcat acttcgcttc attttcatct gctcttgata
     5881 cctgttcgcg ttcttcttca tcagcccaaa cttcctctgc tgacggatgg ttgtacatgc
     5941 gcttatccgg aggaatgaac tcgaaccacg catggatgcg ataacgcaca tcacgcaaaa
     6001 aaagtccggc tctctgttgt cggcaatcct cgatataaag gcccatagga tcgctgcttc
     6061 ttccttccat atgcgcctgc gtggggctat tctcaatttt tcccacctca tttcgccgca
     6121 cagatatcca tctgatcggc cttaatacct cgatctgcct tatgtgccac ttaatggcag
     6181 gcttccagag aatagcttca aatatcgcac gcgcagctga aggagtcatg acatcgtagc
     6241 ttacccgctc aactttcatt tcaggccgtg tgaagcaggc atattctccc catacttcaa
     6301 gacaccagtc tttcatgtcg cctccttttt atatagcgat aaatgatttg gggtcatttg
     6361 gcatctcatc aatgagaagt ccaacaacgt cgtcgtattc tattttgcag gtaagagcaa
     6421 agagaccggg gcttacttcc tcgatagaac ctcgatttag cagagcataa aactggtcat
     6481 tatagatggt tacggaatac ctttggagtt tgcggagaag ccagcgctcc ggccctttgg
     6541 ctttcaattc tgcaatgaac ttttctcctt ccccataagg gacaagaact gtcttttgca
     6601 tggcatcatc tatgatatga aatgcttctg cggcggaacg gaactgtatg ccacaatctt
     6661 gcatatcagg cgagagcatt ttcataatcc ccttttcatc gagagaattt actttccagt
     6721 acaattcgga aaagtactga ctaaaggcag catgatctat gggatcctta agtcctgaat
     6781 ccagaagcct tgcagtcgtc tcgcttgcct tacgcaaaat ccctgctggc ggttttcgtt
     6841 ggggagtgaa gacaacgact ttccctagtc caccatttgt ttttaacttc ccctctctgt
     6901 tgcatctgcc tgccgattgt gcgatggaat cgaggcctgc catagcccta taaacgactg
     6961 ggaaatctat atctactcca gcctccacta attgtgtgct tatcactcga acaggctctg
     7021 ttgttttcag cctttccttg atctcggcga tacagtcgct tcggtgttgg gcgcacatca
     7081 atgcggagag atgataggta ccctttggca taagggcatg aagttccctg cagcttcttc
     7141 tgtctgaaac aacacagagg accctctcaa aggactttag ttcttccgcg atttcttccc
     7201 atcttctggt tgtgtttgta tcaacaggat ctacttcaac ccgccttagc tcgcgataga
     7261 gcctagggac atcttgtata atctctctga cacttcctct ggggagaccg ggaaactgag
     7321 gataatcatc ttgagcctcg aaggctggct gcgtggccgt acataaaacg aaagttacct
     7381 tgtaatgttc acagagaagc ttcatggtcg cgagaattgg ctcaagatat cccgtcggga
     7441 tgagttgcgc ctcatcgagc acaactacgc tgtttacgat attgtgcaat ttgcggcacc
     7501 gacttggttt ggctgcaaaa agcgattcaa aaaactgaac tgaagtagtt acaataatcg
     7561 gagcatccca attttctgct gccaaacgtg attttggact tgaatcattt tcatctaaat
     7621 tggaatggtg ctcaatgacc tgttcctcac caagcacctt tcgaaataca tcagcattct
     7681 gctcaataat gcttgtatat gggatcacat aaataatacg ctctttccca aatttcgcag
     7741 catgttcaag cgcaaaagcc atgctcgaaa gggtcttacc tccgcctgtt ggaactgtta
     7801 aggaaaagaa tcctgccttc gattttgctg ctgaacggca ttcaacaagt attttctgtc
     7861 gtgcttgatt tacaattgta ttttcttctt caatagtctt ttttatcatt ttatccatat
     7921 atgaattgaa ctggatcaat aattcagcta aagacaaata gtcccctctc gtttttttat
     7981 tatctggttc catataggat tcagtatcaa gaaaatctgc atccacaata cacgagaata
     8041 tcatccttat ccaaagcgac atgtcgaggc ttctattgtc aaatttccat ggggggacaa
     8101 aagatccaga aaacctagga tccttcctat attcctcagg aatgctgcaa gtttttatat
     8161 tctgtaatct aaatgacaat gagctcggag aacctatcca gtcaggtaac cctgaatgat
     8221 ggccagctat gcaatatgat aatattcggc ctgtagcttt gccaaattgt tcctcaacga
     8281 attttgcact tggcgaagaa tgatcttgtt tgcctgcttt aatctctagg tgagcttcat
     8341 catcgtagcc gcttttaact cttaaataat gctgccaaat agacgctgct ttgcctatat
     8401 catgtgtaaa accagccgca tatgcccaag aagcagagtt gaatgcttca gcaaatctgg
     8461 aagctagctt tgcacttcct tctagatgat ctatgagtgg ttgtggattt gcccatgtcc
     8521 catccttatt ttttcttaca tgtgcaatta attcggggtt attcattttt cctacgcccc
     8581 tgcgacttca ttatcatcag attttgagcc aggagtttgc tatagagcat cattcgtctc
     8641 cgattttaga tatacatgca aaccattata tcatcttaat ttcctgccgc aaggatttca
     8701 tattacatat aataaaatca cagtgatctc cccaaaatat caaactcacc tttgcaccac
     8761 tcaaatcgat agtttaagcg aatcagaacc cagaaagaaa aactttcctt ctaatatcgc
     8821 actttgcctc gccaagccat tgaggtatgc cgcgatatcc atttgattga tacttttctt
     8881 tgcaaagtca aacagttcag cccagatata gatcgcttta cagaaagagc agtaacgaat
     8941 gaaaaaggcc aatatgtctt cttctggagt agtacaagga catagttgac gtatagtagg
     9001 atgcgagata tcagattggt agattaggag ctttccgaaa caattctgga agctgagatc
     9061 tattcgaggg gtttcttcaa ttagactgaa ggccatattc tcagcttttg taagtgcatc
     9121 ctcgccagag aacatatcaa cataatcaaa agctccagat tgtctctcga aaaacagctc
     9181 cacataatct tttctgaatt ggataagata gacaactttt gcgttcatgg cctgggcggg
     9241 cctcctttta caagccaaat atattattag aagtgggaca ctagttgtcc cgtagctgaa
     9301 gaatttagaa cttatgaatt aaggagttgt tttctcgtta gattcttcat ttctccccta
     9361 ccttctccac catcctcttt atctcttctt cccacagctt tcggaattct ggcggtccga
     9421 gcacttcgca gtttgaaccg cagcggaggg cacggccaag gatttcggtc cagttgtgga
     9481 cgggcaggga gaggtcgatg gcgtcggggt ctgtagcatc gggaactggg tcaattatct
     9541 gatccttgtg ccactgttgg attctgatgg cacgggctgc tcccccaaag aatcgcaagg
     9601 tcgctatgcc gagcacttcg cctttgaaaa tgccatatga ggatgaaata tagctttcga
     9661 ttgcgtcatc gcttggaatt gcaaagcagg gtgcgcccgc ggctctcaca ttgtccattc
     9721 gcgatataga gaatgtcctc agctccat
//
LOCUS       DAOOWY010000067         9734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_6974_length_9734_cov_11.710817, whole genome shotgun sequence.
ACCESSION   DAOOWY010000067 DAOOWY010000000
VERSION     DAOOWY010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6974_length_9734_cov_11.710817"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..638)
                     /locus_tag="PK105_06100"
     CDS             complement(201..638)
                     /locus_tag="PK105_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU61318.1"
                     /translation="MIEVDQLQKYSLFGGISPEQIERIKPLLGTEHFEAGECPQVEGQ
                     PNDKIHFILSGEVEIIKKGLVIARLREGQTFGEMELLDIMPSIATVRAATALETVTIS
                     NRALYDIFKLDLRAFSMMIMNLARDLSRRLRRMDDIASSQGKC"
     gene            complement(670..1623)
                     /locus_tag="PK105_06105"
     CDS             complement(670..1623)
                     /locus_tag="PK105_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61319.1"
                     /translation="MTFEVFLQMFPIALMFSAPIILTALGGLFSERSGIVNIALEGIM
                     MVGAFTGATITVLLENSTPAAAWIGLLGGLLSGVVFSLLHAVASINLKADQVISGTAL
                     NILAGGLTVYLCQIIFHQQRTRAFSYGIKKLSVPGLSKIPVLGRMFFDQVYPTFYIAI
                     AIVLLTWFVVYKTPLGLRMRACGEHPQAAASMGINVSKMRYFGVLISGALGGLGGAVM
                     VLTADIQYTLVSIHGTGFISLASLVFGRWNPFGVLGAGFFFGFSTALSFYAKDIPLLS
                     GLPGEFFYILPYLLTIIALLFFAGKSVGPKAAGQIYESGER"
     gene            complement(1620..2714)
                     /locus_tag="PK105_06110"
     CDS             complement(1620..2714)
                     /locus_tag="PK105_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61320.1"
                     /translation="MPARKEKIVLPLIAVLLGFLLGSLIVILTGRSPVSMYAAIIKGF
                     SGIDIINGQPINTRYIGEFIIQAMPIILTGLSFAFASRTGLFSIGAEGQLMMGSISAT
                     AVAILVQAPKVIHLPMVLLAAILGGALWGAIPGFLKARYNVHEVVVTIMMNYIALHFN
                     NYMILNIFGSVDKVKTVPFPDSALLKSPFLESITRNSRLNWGFIPVLLAVLIFSFIIN
                     KTTFGFSLRAVGHNKEAARYAGMKVNRNIIASMAIAGAFAGLAGGIITVGTFNFGRVI
                     QAAEGYGFDGIAVALVGGNEALGILLAGLLFGGLKAAQPLMQSQGIPREIAGIIQASI
                     VLFVAMKFGIKMVLDKFERKQVPTTEGERV"
     gene            complement(2733..4289)
                     /locus_tag="PK105_06115"
     CDS             complement(2733..4289)
                     /locus_tag="PK105_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61321.1"
                     /translation="MPNIVEMLHITKDFPGIRANDDITLQVEERSIHALLGENGAGKS
                     TLMSILFGLYQPDAGTIRIRGKEVKVSDPNVATALGIGMVHQHFKLVHNFTVTENIIL
                     GMEPRKGILVDMHKAEQKVEEISKLYGLAVDPKAKIDEISVSMQQRVEILKMLYRDAE
                     ILIFDEPTAVLTPQEIRELMQIMRRLVEEGKTILLITHKLREIKEVADVCSILRRGKL
                     VGTVPVADTDEERMAEMMVGRQVSFHVQKTRAKPGDVLLRVEGLNVKNQKGVLGVRNL
                     SLSVRAGEILGLCGIDGNGQTELVHAITGLTKVESGHIFLRDIDITHLSINERSDLGL
                     GHIPEDRQKHGLILDFTLGENFVIHNYYKEPFAHWGILNKKAILDNAQRLIREFDVRS
                     GQGPLTPARAMSGGNQQKAIVAREIDRSPDVFIVVQPTRGLDVGAIEYIHKRIIGERD
                     KNKAILLVSLELDEIMDLSDRIAVIYNGEIVGIVDAATTNENELGLMMSGALHKAHEA
                     TQTGREPAHV"
     gene            complement(4568..5572)
                     /locus_tag="PK105_06120"
     CDS             complement(4568..5572)
                     /locus_tag="PK105_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU61322.1"
                     /translation="MKKALLVLVVTALIAVPAFAQFKVGLVTDVGGIDDRSFNQGTWE
                     GIVRFAQDYKLPKSNYKYLQSSAEADYVPNLSTFADEGLNLIIAPGFLFEQAMTQVAK
                     NYPTRKFLIIDTVVSLPNVVSATFAEHEGSFLVGVAAALKAKADGKNILGFLGGMQFP
                     LIEKFQAGFEQGVKAVYPDCKILVDYAGDFAAPDKGQALAQKQYNAGAYIIYHAAGGT
                     GNGLIKEAKERQQKGDLRWAIGVDKDQYADGIYSGNKSAVLTSMMKRVDVAAYEVSKM
                     TMEGKFPGGQVLVFSLKNKGVGIPDNNPNLSADIVAKVKEYEALIASGKLVVSEIPAK
                     "
     gene            complement(5694..6395)
                     /gene="deoC"
                     /locus_tag="PK105_06125"
     CDS             complement(5694..6395)
                     /gene="deoC"
                     /locus_tag="PK105_06125"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOU61323.1"
                     /translation="MKKKWTSLMLAKVIDHTLLKATATEDQIKALCVEAKEFGFKAVC
                     VNPYWVPLCVRELADSDVLVATVVGFPLGANASLIKAEEARLAVAQGADEIDMVINIG
                     KAKAGDWAAVRDDIAGVVQASRPAIVKAIIETCYLTQEEKIAACEAAISAGATFVKTS
                     TGFGTGGATVEDVSLMKRTIGEVAHVKASGGIKTCEDVVSMLEAGASRIGASAGVAIM
                     RESEALDNKSPEQLS"
     gene            complement(6432..6986)
                     /locus_tag="PK105_06130"
     CDS             complement(6432..6986)
                     /locus_tag="PK105_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61324.1"
                     /translation="MNKTAMLRLKRMHLAPYIQLATSLIGKPRRSGGNMFRHQLDTMA
                     ILIDYGYIDSVLLKASLIHDLLEDAPETNPDSILSIDDESIDVYKLVLEVTRRPVENK
                     AEFLIRIRDYGTPHARVLKSADRISNMISLGYVTDDVFVRRYTDETEQYIYPIAERAD
                     ERMLMELRELVATRREYLRNRIEI"
     gene            complement(7064..9721)
                     /locus_tag="PK105_06135"
     CDS             complement(7064..9721)
                     /locus_tag="PK105_06135"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOU61325.1"
                     /translation="MKSVELAKAYDPKSFEERIYEMWKREGLFKPKIDKNKRPFTIVI
                     PPPNVTGALHLGHALDNCLQDIQVRYRRMKGIPTLWLPGTDHAGIATQNVVERKLRKE
                     GRDRESMGREAFIEETWKVAREHRAFIDNQLARIGSSLDWSRERFTLDEGLSKAVREV
                     FVALYERNLIYRGEYLVNWCPSCGTALSDDEVEHEDEPGSLWYIWYELVDGPCRECPS
                     GRIEIATSRPETLLGDAAVAAHPDDNRYRAIIGKKVHLPLTDRIIPIIEDSYVDPEFG
                     TGLVKITPAHDPNDFLVGQRHGLENINILNPDATLSDAVPEKYRGLSVLDARKAVLAD
                     LKAQGLLKNEEKIVHAIGHCYRCHSAIEPYLSKQWFVRMQPLADKALKAWQDGDVVFF
                     PKKWEHTYRHWLENIRDWCISRQLWWGHRIPVFYCIDCGEMMVCREDPSVCIHCGSSN
                     IYQDEDVLDTWFSSWLWPFSTLGWPEDTEDLRYFYPTSALVTGYDIIFFWVARMIMAG
                     MEFTGQSPFKEVYIHGLIRDKQGRKMSKSLGNGIDPLDIVEEYGADALKFTLAYNCAA
                     GQDILLDKESFKLGSKFANKVWNASRYILMNLENRAFLEDDALTYSDIDWWILHKLNE
                     AAKAAIQAFESWRFNDAAQAVYSYFWNDFCDWYIEASKLSTKSGSESEKDRSTTMLLR
                     VLEESLRLLHPFLPFVTEEIYGMLPNAKGRLIIASYPVWTKDRESPEIESQFKALQEI
                     VTLVRTLRSEFQIAPEISIPLSLEFDNHFSHGQFIQSHIELISLLAGASSVSISKATE
                     KGSVSLVGNGFTVHVGVGGLLDIARLINRLSKESERERAYIEKLRAKLGNRTFHSSAP
                     SDIIEKEKEKLRLSMAKAAKLEQYIEELS"
BASE COUNT         2511 a         2442 c         2138 g         2643 t
ORIGIN      
        1 ttaaaatccc ttgctgcgca agcggcgtgc gggttcgatg cccgtctccg gcaaaagata
       61 ttatttaatt attttataat aatttataaa attgtactat ttattgccta caagatactc
      121 ccttcttccc tattttaaga aggtgctcat ttgtcggtta agaaaagaat tattggcttt
      181 gggattcttt gcatcgaatc tcaacatttt ccttggctgg atgcaatatc atccatccgt
      241 cgcaagcgcc gcgaaagatc gcgcgcgaga ttcattatca tcatcgagaa agctctcaga
      301 tcgagtttga agatatcgta gagggcacga ttagagattg taacggtttc aagagcagtg
      361 gccgcgcgaa ctgttgcgat actcggcatg atatctagaa gctccatctc gccgaaggtt
      421 tgcccttccc ttaggcgggc tatgactagc ccctttttta ttatttcaac ttccccgctt
      481 agaataaaat gaatcttatc gttgggctgc ccttcgacct gtggacattc tccggcttcg
      541 aaatgctctg taccaagcaa aggcttgatc ctttctatct gttccggact gatgcctccg
      601 aagagcgagt atttttgaag ctggtcgacc tcgatcattt cttgcttcct tcagaatatt
      661 atgcttgttt caacgttccc ctgattcgta gatctgtcca gcagccttgg gtcccaccga
      721 tttgcctgca aagaataaaa gagcgatgat ggtaagcaga taaggcagaa tatagaaaaa
      781 ttctccagga agcccagaga gcagcggaat atctttcgca tagaatgaga gggcagtaga
      841 aaacccaaaa aagaatccag ctccgagcac tccgaaggga ttccatctac cgaagaccag
      901 cgaagcgaga gaaataaaac ccgtgccatg aatagacacc aaggtatatt ggatatccgc
      961 cgtcagtacc attacggctc cgccaagtcc gcccaaagca cccgaaatca gaacgccaaa
     1021 atagcgcatt ttggaaacat tgatacccat gctcgcggct gcctgaggat gttcaccaca
     1081 agccctcata cgaagaccaa gcggcgtctt atataccaca aaccatgtca aaagtacaat
     1141 ggcgatggca atgtagaacg tagggtacac ctggtcgaaa aacatccttc caagcacagg
     1201 aatcttgctc aatccgggga cactcaactt ctttatgcca taagaaaatg cacgagtcct
     1261 ctgttgatga aaaatgatct ggcaaaggta gaccgtaagc cctccggcca gaatattcag
     1321 ggctgtacct gaaatgactt gatcagcctt gagattgatg ctcgcaacgg cgtgtagcaa
     1381 gctgaagacc acccctgata gaaggcctcc gagcaatcca atccacgccg ccgcgggagt
     1441 cgaattctcc aaaaggacgg ttatggtagc gccagtaaag gcacctacca tcattattcc
     1501 ttccagagca atattgacga tgccggatct ttcgctgaag agacccccta gggcagtcaa
     1561 tataatgggc gctgaaaaca tgagcgcaat aggaaacatc tgaaggaata cctcgaaggt
     1621 catacgcgtt ccccttccgt tgtgggcacc tgttttcgct cgaatttatc gagcaccatt
     1681 tttatgccaa atttcattgc aacgaataac acaatcgagg cctgaatgat ccctgctatc
     1741 tctctgggta tgccctgaga ctgcatgaga ggctgtgcgg ccttcaaccc tccaaacaga
     1801 agaccagcaa gcaagatccc caatgcctca tttcctccca ctagagccac ggcaattcca
     1861 tcgaaaccat agccttctgc tgcttgaatc acacgaccaa aattgaaagt gccaacagtg
     1921 attattccac ccgcaagacc agcaaaagca ccagctattg ccatagatgc gatgatgttt
     1981 cggttcacct tcatgccagc atagcgagcg gcctccttgt tgtgacctac ggcgcgcaaa
     2041 gagaagccaa aggtagtctt atttatgata aaggagaata tcagaaccgc taatagaact
     2101 ggaatgaatc cccaattgag gcgtgagttc cgagttatgc tctccagaaa tggcgacttg
     2161 agcagagcgg aatcggggaa aggcactgtc tttaccttat ccacgctccc aaaaatgttc
     2221 agaatcatat aattattgaa atgcagagca atgtaattca tcattatggt aactactact
     2281 tcgtgtacgt tgtatcgcgc tttcaaaaag ccgggaatgg caccccaaag cgctcctccc
     2341 agaatagccg caagcaacac catcggcagg tgaatcacct tgggtgcttg cacaagaatc
     2401 gcaactgccg tagccgagat ggaacccatc atcaactgac cttcggcacc aatgctgaaa
     2461 aggccggtgc gcgacgcaaa ggcaaaagat agacccgtta gaattatcgg cattgcctgg
     2521 attatgaatt cacctatata acgcgtgttg atcggctgtc catttatgat atctatgcct
     2581 gaaaatcctt ttattatagc ggcatacata gagacaggag aacgaccggt tagtatgacg
     2641 attaatgatc ccagcaagaa tccaagaagc acagcaataa gtggaagcac tatcttttct
     2701 tttcgagcag gcatttgcct tcctcccttc cctcagacat gcgctggttc tcggccagtc
     2761 tgagtcgctt catgtgcttt atgaagagct cctgacatca taagaccgag ttcattttca
     2821 ttggtcgttg ctgcatcgac gatgccgacg atctctccgt tgtagatgac agcaatgcga
     2881 tcagagagat ccatgatctc atcgagctca agggagacta acaaaatagc cttgttttta
     2941 tccctttctc caatgatacg tttgtgtata tattcgatcg ctcccacatc gagacctctc
     3001 gtcggctgaa ccacaataaa gacatcgggc gaacgatcga tttcgcgagc cacaatggcc
     3061 ttctgctggt ttcctccaga catcgcgcga gccggtgtaa ggggtccctg cccagaacga
     3121 acatcgaatt cccggataag cctctgagcg ttatccagaa ttgccttctt gttcagtatc
     3181 ccccaatgcg cgaaaggttc tttataatag ttgtgaatta cgaaattctc gcccaatgtg
     3241 aagtcgagga tgagcccatg cttctggcga tcttctggta tatgtccaag accaagatca
     3301 gacctttcat ttatagaaag gtgagttata tcgatatctc gaaggaagat atggccagat
     3361 tcaaccttag tcaatccggt tattgcatgt acgagctctg tctggccgtt gccatctata
     3421 ccacagagcc cgaggatttc gccagcgcga accgagagtg atagatttct tacgccaagt
     3481 actccttttt gattcttcac attgagcccc tcgacgcgca gaagtacatc gccaggcttt
     3541 gctcgagttt tttggacatg aaaactgacc tggcggccga ccatcatttc ggccattctc
     3601 tcttcatccg tatctgcgac aggaacggtg ccgacgagct tacctcttct cagaatagag
     3661 catacatcgg cgacttcttt tatttctctt agcttgtgag tgatgaggag aatggtcttg
     3721 ccttcttcga ccagtcgcct cattatctgc atgagttcgc gaatttcctg tggtgtaaga
     3781 acagctgtag gttcatcgaa gatgagaatc tcggcatctc gatagagcat tttgagtatt
     3841 tcgactcgct gctgcatgct caccgaaatc tcatcgatct tcgccttagg atcgaccgca
     3901 agcccataga gcttggagat ttcttctact ttttgttctg ccttatgcat atcgacaaga
     3961 ataccctttc gaggctccat gccgagtatt atgttttcgg taactgtaaa gttatgaacc
     4021 agcttgaaat gctggtgaac cataccaata ccgagagcgg ttgccacatt ggggtcactt
     4081 acctttacct ctttgccgcg aatgcgaatt gttcctgcat caggctgata tagcccaaaa
     4141 agtatgctca tcagcgtaga tttcccagca ccattctctc ccagaagtgc atgaatcgag
     4201 cgctcttcga cttgaagtgt gatgtcgtca tttgctctaa ttccgggaaa atccttggtg
     4261 atatgaagca tctctacgat gttaggcacc tataaccccc ctggattagg atgaagcaaa
     4321 attatatcac agcgcgacta ttctcgctat ataaccagca cacattatac atcggtcaaa
     4381 gaccgatttt gaaatgattt ctttatggaa tcgccaattc aagatgaaac gcatcaaaaa
     4441 agccccctct tttgggggct tttgaaacaa ctatagagga agccgtccac acagagctgc
     4501 tttttcggca attttgagct ttagcagatc gccgattcgg gcgcttcccc ctttttgctt
     4561 agactgctta ttttgcagga atctccgaga cgacaagctt gcctgatgct ataagtgctt
     4621 cgtattcctt gacttttgct acgatgtcag cggagaggtt tgggttatta tcaggaatgc
     4681 caacaccctt attcttcaat gagaaaacaa ggacttgccc gcccgggaat ttcccttcca
     4741 tggtcatctt gctcacttca taggctgcca catcgacacg cttcatcatc gatgtaagga
     4801 ctgcggattt gttgccacta tagatgccat cggcatattg atccttgtcc acgccgattg
     4861 cccagcggag atcgcctttc tgctggcgtt cctttgcttc cttgatgagt ccgttaccgg
     4921 taccgcctgc tgcatgatag attatgtagg ctccggcgtt atactgcttt tgagctaaag
     4981 cttgaccttt atcgggggca gcaaaatccc ctgcatagtc tactaagatc ttgcagtccg
     5041 gatagactgc cttgacaccc tgctcgaatc ctgcctggaa cttttcgata agcgggaact
     5101 gcatgccacc caggaatccg aggatgttct taccatcggc cttagccttt agggctgccg
     5161 ccacacctac caggaaagat ccttcatgct ccgcaaaggt agcggatact acgtttggca
     5221 gagatacgac cgtatcgatt attaagaact tgcgggtagg gtaatttttt gcgacctggg
     5281 tcatcgcttg ctcgaacagg aatccagggg ctatgatgag attgagacct tcatcggcaa
     5341 aagtggatag gttgggaaca taatccgctt ccgcgcttga ctgcagatac ttgtagttgc
     5401 tctttggaag tttgtaatct tgagcaaaac gaacaatacc ttcccatgta ccctggttaa
     5461 aggacctgtc gtctatacca ccaacgtcgg taacgaggcc cactttgaat tgtgcaaatg
     5521 cgggcaccgc gatcaaagcg gttacgacaa gcaccaaaag cgcttttttc atgcttcatc
     5581 ctccttcatt ataggttcga tgctggaaat gcgtagattc tctatcaatc taccgcataa
     5641 aatcaaataa atggtaaagc cgaggcagca gattgtcaat aacaatcttt gcgttatgac
     5701 agctgttctg gcgatttatt atctaaagct tcagactccc tcattattgc aactcccgcg
     5761 gaggctccta tgcgcgaggc cccagcctct agcatcgata caacatcctc gcaagtcttg
     5821 attccgcctg atgcttttac gtgagcgact tcgcctatgg ttctcttcat aaggctgaca
     5881 tcttctacgg tagcaccacc ggtcccaaaa ccggtgctgg ttttgacaaa agttgctcct
     5941 gcggaaatgg ctgcttcgca tgcagcgatt ttttcctcct gggttaggta acaggtctct
     6001 attatagcct ttacaatggc tggtctagat gcctgtacca ctccggctat atcatcccta
     6061 accgcagccc agtctccggc tttagccttg ccaatattga tgaccatgtc gatctcgtcg
     6121 gctccttgag ctacggccaa ccttgcttct tcggctttta tcagactggc gttagcgcca
     6181 agcggaaaac caacgactgt cgcaacgaga acatcggaat ctgcaagctc tctgacacaa
     6241 agcggaaccc agtaaggatt gacacagact gctttgaatc caaactcctt tgcttctaca
     6301 cagagcgctt ttatctgatc ttcggttgcg gtagccttga gaagcgtatg atcgattact
     6361 ttagcaagca ttaggcttgt ccacttcttt ttcatgttac tttccttctg ccctcatcgg
     6421 cgccttcttt attatatctc aattctgttc cgcaaatact ctcgacgagt agcgacgagc
     6481 tctctcaatt ccatcagcat tcgctcatct gcccgttcgg caataggata gatatattgt
     6541 tctgtctcat cggtatatcg cctaacgaat acatcgtctg tcacataacc aagactgatc
     6601 atgttggaaa tgcgatcagc gctcttcagc actcttgcgt gcggtgttcc ataatctcga
     6661 attcgaatta agaattcggc cttgttttcg actggccgtc ttgtaacctc gaggaccaat
     6721 ttatatacat ctatggactc atcatcaatc gaaagaatgg aatcaggatt cgtttccggt
     6781 gcgtcttcga gtagatcatg gattagagaa gcctttagaa gcacagaatc gatatatcca
     6841 taatcgatta ggatggccat agtgtccagt tgatgcctga acatgttgcc tccgcttctc
     6901 ctgggctttc cgattaagga tgtcgctagc tgaatatatg gcgcaagatg catacgcttt
     6961 aggcgcagca tcgctgtctt attcattatc aggcgctcgg cacgaagtgt tgaattccgc
     7021 cctatgatgg gaatgtctac ttgagggata ccctcttctg aactcatgag agctcctcga
     7081 tatactgctc gagctttgca gcctttgcca tagatagcct gagcttttcc ttttcttttt
     7141 cgatgatatc tgagggagca gaggaatgga atgtccgatt gccaagtttt gctctgagct
     7201 tttcgatgta tgctctctct ctttccgact ccttagaaag gcgattaatc agtcttgcga
     7261 tatcgagaag acctcccacc cccacatgga cagtaaagcc attacctacc aaagaaacac
     7321 tccccttttc tgtggccttg cttatcgata ccgaagaagc acctgccaat aggcttatga
     7381 gctcaatatg actctgtatg aattgaccat gcgaaaaatg attatcaaac tcaagagata
     7441 gaggaattga aatctcgggc gcaatctgaa actcacttct aagtgtacgc accaaagtaa
     7501 caatctcttg aagtgcttta aattgtgatt cgatttcagg cgattcgcga tcctttgtcc
     7561 acacgggata ggatgctata atcagccgtc cctttgcatt tggtagcatg ccatagattt
     7621 cttcggttac aaacggaaga aagggatgca aaagcctgag tgactcttcc agtactctaa
     7681 ggagcattgt agtagagcga tctttttctg attcgcttcc cgatttagtc gataattttg
     7741 atgcttcgat gtaccaatcg cagaagtcgt tccaaaaata ggaatagact gcttgagcag
     7801 cgtcattgaa acgccaggat tcgaaagcct gaatggctgc tttcgcggct tcgtttagct
     7861 tatgcaaaat ccaccaatct atgtcgctat aagtgagggc atcatcctca agaaaagcac
     7921 ggttctcgag attcatcaga atatagcgcg aggcattcca aaccttatta gcgaatttac
     7981 tgcctaattt gaaactttct ttatcgagga gaatatcttg gccagcggcg caattatagg
     8041 ccagggtgaa tttcagtgca tctgcgccat attcttccac tatatcgagc ggatctatgc
     8101 cgtttcccag actcttggac atctttcggc cttgtttatc gcgaattagg ccatgaatat
     8161 agacttcctt gaagggactc tgacctgtaa actccatacc ggccatgatc atcctggcta
     8221 cccagaaaaa aatgatatca tatcctgtca ccaaagcgct tgtaggataa aaatatctca
     8281 ggtcttccgt atcctcaggc cagccaagtg tactgaaagg ccaaagccaa cttgaaaacc
     8341 atgtatcgag gacatcttca tcttgatata tgttgctgga gccacagtgt atgcagacag
     8401 aggggtcttc acggcaaacc atcatttcac cgcaatctat gcaatagaaa accggaatgc
     8461 ggtgtcccca ccaaagctgg cgtgagatac accaatccct gatattttcc agccaatggc
     8521 gataggtgtg ttcccacttt ttcgggaaga agacaacatc tccatcctgc caagccttta
     8581 gagccttatc agcaaggggt tgcattctaa caaaccactg cttcgagaga taaggttcga
     8641 tggcgctatg gcatctgtag caatgaccga tcgcatgcac gattttttct tcatttttta
     8701 gaaggccttg tgcttttaga tcagcaagca ctgctttgcg ggcgtcaagt acacttaaac
     8761 cgcgatattt ctcgggcaca gcatcagata gcgtcgcatc tggattgaga atattgatat
     8821 tctcgagtcc atggcgctgc ccaacgagga aatcgttcgg atcatgagct ggtgttattt
     8881 tgacaagacc tgtgccgaat tctggatcga catagctgtc ctcgattatg gggattatac
     8941 gatctgtaag aggcaaatga actttcttgc cgattatagc acgatagcga ttatcatcgg
     9001 gatgggctgc tacagcggca tcgccaagca gcgtctctgg acgagatgtt gcgatctcga
     9061 tcctgccact cggacattcc cgacaaggcc catcgactag ctcgtaccag atgtaccata
     9121 gagaacctgg ttcatcctca tgctccacct cgtcatccga aagagcggtg ccacaggatg
     9181 ggcaccagtt caccaggtat tcgccgcgat atatgagatt tcgttcgtag agcgctacaa
     9241 agacttcgcg cacagctttc gataatcctt catccagagt gaatctttcc ctgctccaat
     9301 caagactaga tccaattctg gcaagctgat tatcgataaa ggctcgatgt tctcttgcca
     9361 ctttccatgt ctcttcgatg aaagcttcgc gccccataga ctcacggtct cgtccttctt
     9421 tacgaagctt gcgttccacc acattctgag tagctatacc cgcatggtct gtaccaggca
     9481 accatagtgt cggaattcct ttcatgcgac gatatctaac ctggatatct tggagacagt
     9541 tatctagtgc atgccccaga tggagagccc ctgttacatt gggaggagga attacaatgg
     9601 taaagggtct tttgttcttg tcgatctttg gcttaaaaag gccttcgcgc ttccacattt
     9661 cgtagatgcg ctcttcgaat gatttggggt cgtaagcttt tgcaagctcg actgatttca
     9721 tgctctcctc cgca
//
LOCUS       DAOOWY010000068         9578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_7099_length_9578_cov_9.186811, whole genome shotgun sequence.
ACCESSION   DAOOWY010000068 DAOOWY010000000
VERSION     DAOOWY010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7099_length_9578_cov_9.186811"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1237
                     /locus_tag="PK105_06140"
     CDS             23..1237
                     /locus_tag="PK105_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HOU61326.1"
                     /translation="MIIKPMIRNNICLNAHPEGCRKEVRLQAARVMNFRERLSKSSIN
                     DSSAKPNHALIIGCSTGYGLASRLVAAFGYGAETVGFSYEKEPSATKPATPGWYANKE
                     FDRLAQEKGLFAASFSQIDAFSHTAKQKAIEIALENKFKYDLVIYSLASSVRVDPDTG
                     VLYRSVIKPIGRQYAGRTIDIFTEKFSEASVQPATEEEITQTIKVMGGEDWQLWIDAL
                     KKKDLLAPEALTLAYSYIGPPFSWPIYRDGTIGQAKLHLEETAKLLEKNYGKDNGLRS
                     FVSINKAVVTRASAVIPIIPLYISTLFKVMKERNIHEDCLDQMMRLFSERLYQKRGTG
                     IPTDEEGRIRIDDLEMGPSVQNEISSRLARIDESNLHELADVKGFRSDFLRAHGFDVP
                     GVDYERDIVSFE"
     gene            1234..2013
                     /locus_tag="PK105_06145"
     CDS             1234..2013
                     /locus_tag="PK105_06145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOU61327.1"
                     /translation="MNILILDSSSETQFLSLKDRRTQHIIEVLKKEVGGRMRAGSLNG
                     LVGWAILESIGNTEIELRFVAEGTAPPLRPLRILIGTVRPIQAARIVKELAILGVESI
                     VFLPTELGEKSYTQSHFYQQKEYLAYAREGAEQSGNPRLPEIGLAWSLDRALIKAEHF
                     ESMDHTNTINPESAGEAKINKCTKLLCHPDPYAKLLSEIILSELPVILAIGTERGWTK
                     HEIDIFLNAGYLPCSLGDRILKTETAAFAAVSILLSRLGYM"
     gene            complement(2037..3842)
                     /gene="pepF"
                     /locus_tag="PK105_06150"
     CDS             complement(2037..3842)
                     /gene="pepF"
                     /locus_tag="PK105_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOU61328.1"
                     /translation="MAKTIPERSNVPAEHRWNLASLFSDDSEWERELVTFNAMILDAE
                     KSRLSLGADRKAFLEILKAFEEYLILEERLGYYADLRVTEDEGNSIARGMLERYVNLS
                     TQGQAAWSWLSPAIQMLDDDFFDSCLKDSAFENYRVFLSKLRRFKPHVLSEAEERLLA
                     LQSEAAQTPKDAFSVLTNVDFDFGTIEIPEGPKPLTQSTFAYFMRLPDRSVRRKAYLQ
                     FYASFEAHRHTLAALYAGSVKLDKYRAKVRKYPSARAMALFPDNVPEAVYDNLVSTVN
                     KNIPALHHYYKLRKELLGLDSLRHYDVYNPLVQEIKIERSYEQAVNIVIEALRPLGEE
                     YVSTLRRGLLGGWVDRYENKGKRSGAFSAGSFLGDPYILMNYKEDVLDDLFTIAHEGG
                     HSMHSWYSARNNPFMCYGYTIFEAEVASTFNEQLVFSYLYENSKDDAEKAYLLANRID
                     NTLGTLFRQTMFAEFEQRVHEIAESGEAITVDVLRKEYRNLLERYFGPEMILENISDL
                     EGLRIPHFYNAFYVYKYATGLSAGIALSERVLKGGKKEREDYLDFLKSGGSRFPIESL
                     KLAGVDMSTPEPVEAACRRFAEDVEALSAILKARL"
     gene            complement(3884..4903)
                     /locus_tag="PK105_06155"
     CDS             complement(3884..4903)
                     /locus_tag="PK105_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU61329.1"
                     /translation="MSKVTIKDIAQRAGVSKTTVSFALNHPDRLSKETYKRIMAIIDE
                     LGYVPNPFARSLTTKRLGAIGLLLPQKIGDIFANPHMTQVISGIGEECERREFSLAIL
                     PLIRGRIIEAARKSYVDGLITIGVGPGHEVVSLLKQNHIPFVTIDGEESASIINIGID
                     QKNAAADLMRHVLKRGHQRLAILALEPDTKPSGSEISSIVVQRRLEGFQAVLKDAGLS
                     ITDTRVFLQNCKGSIQSGYESAYHILSLDQRPTAIVAMSDITAIGAMIAARELGLEIP
                     EDVAVAGFDDIPESVLVSPRLTTIHQPDRDKGAEAARMVINMLNGSSGQHIKLPYQLI
                     VRESA"
     gene            4971..6650
                     /locus_tag="PK105_06160"
     CDS             4971..6650
                     /locus_tag="PK105_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOU61330.1"
                     /translation="MNTMTLTIDGYKEISVPVGSSAEEIFAQAGMEREWGTCEHPFPP
                     LAALVNNELTSLSAPIDADCRIAPIYPDSPMGAEVYRHSLCFLLAMAAKNIAPKRRLI
                     VSMAIGNGYYHYFDDEEPVSPSLLESLCLYMREIVAQNLPIRIVLQSWNVVFEYFKHS
                     NQAETFALMEYMNDPFIMLNECQDYRDLHISPLVPRTGLLSVWELIPYRRGILLRFPH
                     TKKPYEMDPFVDIPVLYDIAEEYEQKAKVLNAGSIGALNKINQLGEIQDFVLMAEALQ
                     NKKLASIADIIAEKSDKTRVILIAGPSSSGKTTTAKKLSVQLRALGFKPIHIELDNYF
                     VDRSQTPLDKDGKPDFECLEALDIEFLNRQLLDLFEGKEIELPVFDFKSGIRKPSGTF
                     ISIKENEILILEGIHGLNERLTPQISADNKLKIYVSALTQLNVDDHNRVRTTDYRLLR
                     RMVRDYNFRGHDARQTLAMWSSVQRGERLYIFPFQGDADIAFNSALDYELGVLKVFVE
                     PLLRTVKPHHVEYTDARRIQAFISRISPISPQYVPSDSILREFIGSSVFKY"
     gene            complement(6704..8164)
                     /locus_tag="PK105_06165"
     CDS             complement(6704..8164)
                     /locus_tag="PK105_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOU61331.1"
                     /translation="MKLNLSLDNLSEAFPEDLTPDQRARAQTIFLKTLSDKTHRFYGG
                     KIQVVPKAGLYGFNWFNVWYTPGVSKISTTIRDDNDQSFALSNRGNLVAVVSDSTRVL
                     GDGDCTPPGGLGVMEGKAFLMKYLGGVDAIALCINSRDGDGKPNPDKIIDFVKMLEPS
                     VGAVNLEDISQPNCFKVLDELREACEIPVWHDDAQGTACVTLAGLLNALELAGKKLNE
                     AKIVLLGAGASNTTIARLILADGGRGENMVIFDSKGGLHRNRRDIEADKRYYRKWELC
                     LQTNPRCIETEEEALKGADVLIALSTPGPDTVKREWIRSMAPKSIVFACANPVPEIWP
                     YAAKEEGAYIVATGRGDFPNQVNNSICFPGLLKGALLVRSRKITDHMAIRCAHSIADF
                     AKSRGISSDNIIATMEETEVFAREAADVAMAAVEEGVARLPDTWDNIYSKAKADILAA
                     RSLTQDLMKEGHIAPIPEEMLQEAFDFALSEVRKKP"
     gene            complement(8343..9329)
                     /locus_tag="PK105_06170"
     CDS             complement(8343..9329)
                     /locus_tag="PK105_06170"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOU61332.1"
                     /translation="MADLRTYLMGLQLQNPIIIGASGLTSMPDGVEKAAHSGVGAIVL
                     KSLFEEQILAVLGQEMAGLDLNSHPDAEAFISRTAWEDSTDKYLKLISESKRKVDTVP
                     IIASINCVGQGRWADFAGRIEEAGADALELNIAYVPFSPQISGQEIEKRVLSTVKEAR
                     SVTKLPIMVKLGNHYSSLPYLVHGLSKEGANAAVIFNRFFRFDFNLSTMRLSGAQPLS
                     SETEYHESLRWTSILYKRVGLEIVSSTGIHSADAAIKCLLAGASAVEVCSVIYKQGWG
                     IIGTMLKDMNILMDSLGYSCMDSLQGKLATINADRPEEYHRLQYIKALTDIY"
     gene            complement(9388..>9578)
                     /locus_tag="PK105_06175"
     CDS             complement(9388..>9578)
                     /locus_tag="PK105_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOU61333.1"
                     /translation="MNAKIRDAQNQRIPYMAIVGQREQESGTVSIRLRTGDQESGIPL
                     EVFAARVLEKIQTKSLDL"
BASE COUNT         2571 a         2336 c         2095 g         2576 t
ORIGIN      
        1 gcatagcttc aggagtaccg gaatgataat aaagccaatg atccgaaaca atatctgcct
       61 caatgcccat cccgagggtt gccgtaagga agtgcgtctg caggcagcgc gcgtaatgaa
      121 ttttcgtgaa aggctctcta aaagtagcat caatgactca agcgcaaaac caaatcatgc
      181 cttgataatt ggctgttcta caggatatgg acttgcatcg agactcgtcg ccgctttcgg
      241 atatggagct gagacggtcg gtttttctta cgagaaagaa ccttctgcta ccaaacctgc
      301 aactccggga tggtatgcaa ataaggaatt cgatagactg gctcaagaaa agggtctttt
      361 cgcagcatct ttctcgcaaa tagacgcctt ttctcacacg gccaaacaga aggccatcga
      421 aattgcgctg gagaataaat tcaaatacga tttggtaata tactcgctag caagctccgt
      481 acgggtcgat cctgatacag gggtgcttta ccgttcggta ataaaaccaa taggaagaca
      541 atacgctggc cgcacaatag atatatttac cgaaaaattc tcggaagcgt cggttcagcc
      601 tgcgacagaa gaagagatca ctcaaaccat aaaggtcatg ggcggtgaag attggcagct
      661 atggatcgat gctctcaaga aaaaggattt gctcgcgccg gaagccttga ccctagcata
      721 ttcctacata ggccctcctt tttcttggcc catatatcgc gatggtacaa tcggtcaagc
      781 caaactccat ctggaagaaa ctgcaaaact gctagaaaag aactatggca aggataacgg
      841 gcttcgatcc tttgtttcca taaataaggc agtcgtaacg agagcaagcg ccgtgattcc
      901 gatcattccg ctatatatct caaccctttt caaggtaatg aaggagcgca atatacatga
      961 agactgcctc gaccagatga tgcgcctttt ctctgaaaga ctataccaaa agagagggac
     1021 aggcatccct acggatgaag aaggcagaat tcgtatcgat gatctcgaga tgggtccttc
     1081 ggttcaaaat gaaatcagtt ctcgcctggc tagaatcgat gaatcgaacc tccatgaact
     1141 ggccgatgtc aaaggcttca gaagcgattt tctaagggca catggcttcg atgttcccgg
     1201 tgtcgactac gagcgcgata tcgtctcctt cgaatgaata tcttaatact ggattcatct
     1261 tccgaaactc agtttctctc tttaaaagac cgcagaactc aacatattat cgaagtcctt
     1321 aaaaaagagg tcggtggcag aatgcgcgct ggcagtctta atggattggt tggctgggca
     1381 atcttagaat ctatcggcaa tacagagata gagcttcgtt ttgtggccga aggaaccgct
     1441 cctccgctaa ggcctcttcg tatactcata ggaacagtaa gaccaattca ggctgcccgt
     1501 atagtaaaag agctagccat tctaggtgtc gagagcattg tattcttgcc gaccgaactg
     1561 ggagaaaaat catacactca atcgcacttc taccaacaaa aggaatattt ggcatacgcg
     1621 cgagaaggag cggaacaatc aggaaatcca aggctaccag aaattgggct cgcctggagc
     1681 ctggatagag ccctgattaa ggcagagcac tttgagagca tggatcacac caatacgata
     1741 aatcccgaat cagccgggga agcgaaaatt aacaaatgta ctaagttgct ctgccaccct
     1801 gacccatatg ctaaacttct tagcgagata attctttcgg aattgccggt tatactggcc
     1861 ataggaacag agcgcggatg gacaaagcac gagatagata tttttctcaa cgccggctat
     1921 ctgccttgct ctctcggaga tcggatattg aaaaccgaga ccgctgcctt tgctgcggtc
     1981 tcaatcttgc tctctcgttt gggatatatg tagagtcccc tattacaggc tttctcttat
     2041 aatcttgcct tcaatatagc agaaagagcc tccacatctt cggcaaagcg cctgcaagcg
     2101 gcttctactg gctcaggagt cgacatgtcg acaccggcaa gctttaagga ttctatcgga
     2161 aatcgactac ctccggattt cagaaaatcg agataatctt cgcgctcctt ttttccgcct
     2221 ttcaaaactc tctcggagag agcaatacct gccgacaagc ctgttgcata cttgtaaaca
     2281 tagaatgcat tatagaaatg aggtatgcga agtccttcaa gatcggagat attttctaag
     2341 atcatttctg gcccgaaata tctctcgagg aggtttcgat actcttttct aagcacatcg
     2401 accgtaattg cttctcccga ctccgcaatc tcatgaactc tctgttcaaa ttcagcaaac
     2461 atagtctgtc gaaaaagtgt tcccagggta ttgtcgattc gatttgcaag cagataagcc
     2521 ttttctgcat catctttact gttctcatag agatacgaga aaaccaattg ctcattgaaa
     2581 gtactggcaa cctcggcttc aaaaatcgta tatccataac acatgaaagg attattgcga
     2641 gcggaatacc aggaatgcat cgaatggcca ccctcatggg cgattgtaaa tagatcatcg
     2701 agtacatctt ctttatagtt cataaggatg taaggatctc ctagaaaact acccgcggaa
     2761 aaagcccctg aacgcttgcc tttattctca tagcgatcca cccagcctcc caatagccct
     2821 cttcgcaggg tactcacata ctcctccccc aaaggcctaa gtgcttcgat tactatgttc
     2881 accgcctgtt catagcttcg ctcgattttg atttcctgga caagaggatt atagacatca
     2941 tagtgcctta gagaatccaa accgagtagt tcctttctta gtttgtaata gtgatgcaaa
     3001 gcaggtatgt tcttatttac ggtagagacc aggttatcat aaacagcctc tggaacatta
     3061 tctgggaaga gtgccatagc cctagcgctt ggatattttc tcactttagc tctatattta
     3121 tccaatttta cagatccggc atacaacgcc gcaagcgtat gccgatgcgc ttcaaaggag
     3181 gcatagaatt gtagataggc ctttcttctt actgatctgt ctggcaaacg cataaagtat
     3241 gcaaaagtgg attgtgtaag cggctttgga ccttccggta tctcgattgt gccaaaatca
     3301 aagtcaacgt tggtcagaac gctgaaggca tcctttggag tctgggctgc ctcgctctga
     3361 agagccaaaa gtcgctcctc agcttcactc agcacatgcg gtttgaagcg acgcaatttc
     3421 gaaagaaaaa ccctataatt ctcaaaagca gagtccttaa gacagctgtc gaaaaaatcg
     3481 tcatctagca tctggatggc aggactgagc caagaccagg cggcttgacc ctgggtagac
     3541 agatttacat agcgctcaag catgcctcta gctatgctat ttccttcatc ttcggttact
     3601 ctgaggtctg cataatatcc aagcctttcc tctagaatta gatattcttc aaaggccttg
     3661 agtatttcga gaaaggcctt cctatctgca ccaagagaga gcctgctctt ttccgcatca
     3721 agaatcattg cattgaaggt cacaagttct ctttcccatt cggaatcgtc agagaagaga
     3781 cttgccagat tccatctatg ctctgcagga acatttgaac gctctggaat tgtctttgcc
     3841 attatttctc ccttttctaa agcaatccaa tattatttat atatcatgcc gattcacgca
     3901 caataagctg atatggcaat tttatgtgtt ggccagaaga tccatttagc atatttatta
     3961 ccatgcgtgc ggcctcggct cccttatctc ggtcaggctg gtggatagtg gtcagacgag
     4021 gagaaaccag cacactctcc ggaatatcat caaatcccgc tacagcaaca tcttccggta
     4081 tctccagacc tagttctctt gcagcaatca tagctcctat cgcggtaata tccgacattg
     4141 caacaatcgc agttggcctc tgatcaagcg aaagtatatg gtaagcgctt tcatagccgc
     4201 tctgtatcga gcctttacag ttttggagaa atacccgagt atccgtaatc gacaatccag
     4261 catcttttaa taccgcttga aatccttcaa ggcgtctctg aacaacaatc gaagaaatct
     4321 cggatccgct cggcttcgta tcgggttcca gggccagtat tgcgagtcgc tgatggcctc
     4381 tcttgagcac atgccgcatc aaatctgctg cagcattctt ctgatcgatt ccaatgttta
     4441 ttattgaagc tgattcttcg ccatcgatgg tcacaaaggg aatgtggttc tgcttaagca
     4501 ggcttacgac ctcatgacct ggcccgacgc caatagtgat gagcccatcg acgtaagatt
     4561 ttcttgccgc ttcgattatt ctacccctga tcagaggcag aatggcgaga gagaattccc
     4621 ttcgctcgca ttcctcgcct ataccgctga taacttgagt catgtgcgga tttgcaaaaa
     4681 tatctccaat cttttgaggc aggagaagac caattgcacc gagtcgcttt gtcgtcaggc
     4741 tgcgtgcaaa ggggttggga acataaccga gttcatcgat tatcgccatt attcttttat
     4801 aagtctcttt tgataaccga tcagggtggt ttagcgcaaa agaaacagtg gtcttcgata
     4861 ctcccgcacg ttgagctata tccttgattg tcactttaga catgaggtga attctacaca
     4921 aaaacaataa gctcgtcaaa gtactcgtaa taacctataa tagactagaa atgaacacta
     4981 tgactttgac tatcgatggt tacaaagaga tttccgttcc agtgggatcg agtgcagagg
     5041 aaatctttgc acaggctggg atggaacgag aatggggaac ttgtgaacat ccttttcctc
     5101 ctctagccgc tcttgtcaac aacgaactca cttctctctc cgcaccgatc gatgcagact
     5161 gcagaattgc tccaatatat cccgattctc ctatgggcgc cgaggtatat cgccattcct
     5221 tgtgttttct cctagctatg gctgcaaaaa atatcgcacc aaagaggcga ctcattgtga
     5281 gcatggcaat agggaatgga tattatcact acttcgatga tgaggaaccc gtctcaccaa
     5341 gccttcttga gtccctgtgc ttgtatatgc gcgaaatagt ggcccaaaat ctgcccatcc
     5401 ggattgtcct gcaatcctgg aatgtcgtct ttgaatattt taagcattca aatcaagcag
     5461 agacttttgc tttgatggaa tatatgaatg atcccttcat catgcttaat gaatgccagg
     5521 attataggga cttgcatatt tcacctcttg tgccgcggac cggacttctc agtgtatggg
     5581 agctgattcc gtatcgtcgc gggatattgc tccgttttcc tcatacaaaa aagccttacg
     5641 aaatggaccc tttcgtagac atacctgtgc tttatgatat agctgaagaa tatgaacaga
     5701 aagccaaagt gctaaatgcc ggatctatcg gtgctctaaa taagataaat caattggggg
     5761 agattcagga ttttgttctg atggccgaag ctctccaaaa taaaaaactc gcctcaattg
     5821 cagacattat cgccgaaaaa tcagacaaga caagggtcat tctgattgcg gggccttcga
     5881 gttcgggcaa aaccacaacc gcaaaaaaac tatcggtgca gctgagggct cttggtttca
     5941 aacctatcca tatcgagctc gacaactatt ttgttgatcg aagtcaaaca cccctcgaca
     6001 aggatggcaa gccggatttc gaatgcctgg aggctctcga tatagaattt ctcaatcgac
     6061 agctcttgga cctattcgag ggtaaagaga tcgaacttcc ggtatttgat ttcaaaagcg
     6121 gcataagaaa gccttccggc actttcatca gcatcaaaga gaatgaaata ctgattctgg
     6181 aaggtattca tggtctaaat gagcgcctta caccacagat atccgcggat aataagctca
     6241 aaatctatgt ctcagcactg acacaactaa atgtggacga tcacaaccgc gtgcgaacga
     6301 cagactatcg tctcttgcgg cgtatggtaa gagattacaa ttttcgcggc catgatgctc
     6361 gccaaacgct ggctatgtgg tcttcggtcc agaggggaga aagactatat atcttcccct
     6421 tccagggtga tgcagatatt gcattcaatt ccgcgctcga ctatgagctc ggagtgctca
     6481 aggtatttgt cgagccgctt ctgcgaacag taaagcccca tcacgtcgaa tatacggatg
     6541 cccgccggat tcaggcgttc atctcgcgga tttcgcctat ctcgcctcag tatgtgccca
     6601 gcgattcaat tctgcgtgaa tttattggat cgagcgtatt caagtattag ggtgccaccg
     6661 ctctcaatta gcctccgatt gcggtctctt tgaaagaagt aacctatggt ttctttctta
     6721 cctcggacag tgcaaaatcg aaggcttcct gcagcatctc ctctggtata ggcgcaatat
     6781 gaccctcttt catcagatct tgcgtcaaag agcgcgcagc taagatatcg gccttggcct
     6841 tggagtagat attatcccag gtatcgggaa gtctggccac accctcttct actgctgcca
     6901 tagccacatc ggctgcctcg cgcgcaaaga cttcggtctc ctccattgtg gcaattatgt
     6961 tgtcggaact tatgccgcgc gattttgcaa aatcggcaat agagtgggcg catcgaatcg
     7021 ccatatggtc ggttatcttt ctggagcgaa caagcaaagc tcctttcaag agaccgggaa
     7081 agcagatgga attattgacc tgatttggaa aatcgcctcg acctgtggca acaatgtaag
     7141 cgccctcttc cttggctgca taaggccaga tctcaggcac cggattggca caggcaaaga
     7201 caatggattt tggagccatt gaccgaatcc attcgcgttt gacagtatct ggtcccggcg
     7261 tcgacagcgc aataagcacg tcggcaccct ttagggcttc ttcctccgtt tcgatgcatc
     7321 ggggattagt ctgaaggcag agctcccatt ttcgatagta tctcttgtcg gcctcgatat
     7381 ctcttcgatt cctatgtaag ccccccttgc tatcgaaaat caccatgttc tctcccctac
     7441 cgccgtcggc aaggattaat ctggcaattg tcgtattaga agcacccgca cccagcaata
     7501 caattttggc ttcatttagc ttcttgccgg caagctcaag tgcatttaga agaccggcaa
     7561 gagtcacaca agcggtgccc tgcgcgtcat cgtgccatac gggaatttcg caggcttccc
     7621 tcagttcatc cagcactttg aagcaatttg gctggctgat atcctcaagg tttacggctc
     7681 ctaccgaggg ttccagcatt ttaacaaaat cgatgatctt atcgggattg ggttttccat
     7741 cgccgtctct ggaattgatg caaagcgcaa ttgcgtctac accacccaaa tacttcatca
     7801 gaaaagcctt tccttccatt actcccaggc ctcccggagg cgtacaatcg ccatcaccca
     7861 atacacgtgt agagtcagac acaacggcca caagattccc tcggttggag agcgcaaagg
     7921 attgatcatt atcatctcga atcgttgtcg atatctttga aacgcctggc gtataccata
     7981 cattgaacca gttgaatcca tagagacctg ccttgggcac gacctggatc tttcccccgt
     8041 aaaaacgatg agtcttatct gaaagcgttt tcagaaaaat cgtttgtgca cgagctcgct
     8101 gatccggtgt caaatcctcc ggaaaagctt ccgatagatt gtctagactg agattgagct
     8161 tcatagtttc cttccttttg ctatttgacc cctttagaac aatttaggtc tttcgtctat
     8221 tgtcaattgt cgccgaacaa ttggtcggct ggatatggtc ttatactatc atgcgctttg
     8281 aggtggtgca ataaaaagag aaagcacaac ctattgcgca atcacccgga ctgacatatc
     8341 tatcagtata tatctgtcaa ggccttgatg tattgaagac gatggtactc ctccggtcta
     8401 tcggcattga tagtggcgag tttcccctga aggctatcca tgcatgaata gccaagggaa
     8461 tccataagga tattcatgtc tttgagcata gtcccaatga ttccccagcc ttgtttatag
     8521 ataacagaac atacttcgac tgcacttgct ccagcaagca agcattttat ggctgcatct
     8581 gcactgtgaa tcccggtgga tgaaacgatc tcaagaccga cccgcttgta taggatcgaa
     8641 gtccatcgca gactctcatg gtattcggtt tcggaactaa gcggttgtgc tccggagagt
     8701 ctcattgtac tcaaattgaa gtcaaaccta aaaaaccggt taaaaataac ggctgcattt
     8761 gcgccctctt tagaaagccc atggactaga tacggtaagc ttgaataatg gttaccgagc
     8821 tttaccataa ttgggagctt tgtgacagat cgtgcttcct taactgtgga gagcaccctt
     8881 ttctcgattt cttgtcctga gatctgtgga gaaaatggca cataggctat attcagctct
     8941 agcgcatctg caccggcttc ttctatgcgt ccagcaaaat ctgcccatct gccttggcca
     9001 acacaattga tgcttgcaat tattgggact gtgtccactt ttctctttga ttcgcttata
     9061 agtttaagat atttatctgt gctatcttcc caagccgtac gagaaatgaa agcctctgca
     9121 tctggatgac tatttagatc gagccctgcc atttcttgtc caagcacagc aagtatttgt
     9181 tcctcaaaga gcgatttgag cacaattgcc ccaacgccag aatgcgctgc cttctctaca
     9241 ccatcaggca tactggtaag tccgcttgca ccaatgatta tgggattttg tagctgaagc
     9301 cccatcaggt atgtccgtaa gtccgccatt gtcagctcct tgacctctta ttgtaccaga
     9361 aaaatagcag ggcaagtaga acaaaaatta taaatctagc gatttggttt ggattttttc
     9421 caaaacgcgt gcagcgaata cctcgagagg aatcccgctc tcttgatctc ctgttctcag
     9481 tcggatcgat accgtgccac tctcttgctc gcgctggcca actatagcca tatagggaat
     9541 cctctgattc tgggcatctc gaatcttagc attcattc
//
LOCUS       DAOOWY010000069         9397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_7270_length_9397_cov_13.444766, whole genome shotgun sequence.
ACCESSION   DAOOWY010000069 DAOOWY010000000
VERSION     DAOOWY010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7270_length_9397_cov_13.444766"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PK105_06180"
     CDS             <1..321
                     /locus_tag="PK105_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61334.1"
                     /translation="IGKMVDGVFVPNKRYELEGELREAKKPGPAARAISERLFCGATR
                     LLDSIGEKLGIREDLRKCFPQDYKKILYLAYFLLLEDHSPLSRFPRWAMIHRHPYGAA
                     KDGA"
     gene            311..607
                     /locus_tag="PK105_06185"
     CDS             311..607
                     /locus_tag="PK105_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61335.1"
                     /translation="MEHEYLAYDISSISSYSELIRFVKYGKNKEHDGLPQINLALLYG
                     ESSRLPVYYRSMPGNISDVKTIKQLIDELGYLSIEKVKLMLQCTLSAVLHFKDD"
     gene            672..1235
                     /locus_tag="PK105_06190"
     CDS             672..1235
                     /locus_tag="PK105_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61336.1"
                     /translation="MHLYYDDERAMEQRARFNLLREGIRIPDHETLYQKYYELTQTPV
                     RRVKLNPKQEAITEVERNYGYFALLSNDSKDPLEALKIYRTKDLVEKAIGNLKERLSS
                     RREGVSSESNLSGKLFVQFVALMYVSYIDKLMHDQGLYKTYTLTQLLDELDVIECYSY
                     PGHIEKTGEMTKKQKELYEAFGVTLPA"
     gene            complement(1439..2245)
                     /locus_tag="PK105_06195"
     CDS             complement(1439..2245)
                     /locus_tag="PK105_06195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU61337.1"
                     /translation="MKEYYENKVAVVTGGASGIGLALCELLLSFGAKAVVLADINAEK
                     LKAESARLESAYPGKVLGIQTDVSKQESINAMIQQAAKFGGGQIDFLFNNAGLGLLKA
                     FDETTDADWKFAFDINFFGALYGIRAVLPIMRAQGGGHIANTASGAAFSPMAYQSMYS
                     ATKSALVGLTGSLRYEYWDENIRFSTVIPGTVATAIYDELGGPPKSAITPEESARGIL
                     NGLAANERIVIVTEADRNGAINAFHPDTAKYTDEILLDIARQRRSGKLGF"
     gene            complement(2336..3121)
                     /locus_tag="PK105_06200"
     CDS             complement(2336..3121)
                     /locus_tag="PK105_06200"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOU61338.1"
                     /translation="MFKFEEGKCYKMPAHFGGSYFDPDAKAIYDDVVTLIFSYTTDGE
                     QLSNYVPEGFELTKPELSIEFQQCRQVEWMAGSYYDLVSVAAPVRFNGQRDRLEGSFS
                     LVVWENKTTPILTGNMMGVPKIYADIEDLHILADTYRTRLSFEGSTFLELEMIRHQPL
                     DAEEVKAMNTDINSLGWRYIPKVGAPGADLSQPILFPMHNEPNSAWLGSGKVKWTKST
                     WDQNPMQFYIINALAELPIIDMKPVILTQGREILTEAQGRVLK"
     gene            complement(3147..4334)
                     /locus_tag="PK105_06205"
     CDS             complement(3147..4334)
                     /locus_tag="PK105_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61339.1"
                     /translation="MISREAKLPFEIVMLIIAAVIILITGAMLFPVYSGTLPYYENGL
                     YGLLLIIFALQTITLGRTPFGDTPRSRLLLVVGLLIAIIGMVTSLIPISISFSRVLLF
                     LCLGPGGLLLFLQMLFIKDKLKSWVKYGGIFRHLIFACSAVYILSMVIALLLWKQNLL
                     TTPMTAVVILFFGTAVFYLATVLGKIDRTYPEAAKNPIGDIKLSIEQTMILFTSIFMI
                     IIGVLLIPVNLGMLPFSSSAQLGILMVIFAIQMLAFGNTPIGPFPRTWPMIGLGILFA
                     VLGAISCIIPGILVSVLTILVGILNILGGIIALTKMLPKGSKQKTEPREPIPPILKKL
                     FATQLTLNLLQILFGMSMLIPGIVPGMVIGVILAANGGVLSYLLSILVTLDKMRSNME
                     GTK"
     gene            complement(4469..5206)
                     /locus_tag="PK105_06210"
     CDS             complement(4469..5206)
                     /locus_tag="PK105_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOU61340.1"
                     /translation="MDKKFHQVFWASALLAVALVVMAIIVVNGFVQVRGAQDTISVTG
                     SAKEPVESDISVWNFSVNIRGNSLQAVYADLKTAMPKIDTFLATQGIPAEEIVASAIN
                     AGSNIYFDEDQQGRRTQHVTYFANCSYRVTSTDIDRLERVSRSAGDLIAQGVALDSAS
                     LEYMYTKLADLRNSLLERATEDAKKRAEAIASSSGAKIGAVKSARMGVFQITPRYSTM
                     VDDYGINDTSSRLKDVTAVVGAEFRLK"
     gene            5663..6628
                     /locus_tag="PK105_06215"
     CDS             5663..6628
                     /locus_tag="PK105_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU61341.1"
                     /translation="MKSKMKTIATTLLMLVLCLGIVGAAPKKIVIGITMQGNQSGFIQ
                     YITAGMWEWQKNNAPDVTLKVVFADDDATKQLTQVETFVAQKVDAIIINPVDKVQSAA
                     AVDIAAQAKIPILTVNTTTVSKNATAHVGSDDVEAGYLQMNRIIKVCGEKARVAYVDA
                     VLGHSAQVGRTEGYKKALSEHPGATLVVHDTGNWSADDSMKLVENWIQRGLKIDAIAC
                     MADCQLIGVVTAVKNANLIGKIVLSGMDCDPLIMQYIKEGIVDSSIWQDGVGQGANAV
                     RLAIDAANGKKVQDFMLPFEVCTKDNFDHYMQLAKEREELAKKYF"
     gene            6700..8217
                     /locus_tag="PK105_06220"
     CDS             6700..8217
                     /locus_tag="PK105_06220"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU61342.1"
                     /translation="MRHELNNNVVLQMRHIAMEFDGVQVLDDVNLTLRSGEVQALVGE
                     NGAGKSTLMKILIGMYQPTFGEIECKGNVVRFRNIKDSQAHGISMIFQEFNQVKHLTV
                     MENIYLGREPLTKAGLIDYKAMFKQSKQLLESMGVEISPNMYVRELTVAKQQLVEIAK
                     ALSNKAEILIMDEPTSALSNKEIEHLMNMIGMLKAAGKSIIFISHKLEEIYRICDYVT
                     VLRDGKHIYSGLLKGLDEKDLIKMMVDREITEMYPKEKVNAKDIVLEVKGLTRKNEFR
                     NISLEVREGEILGLAGLMGAGRTEVIETIMGIRKADSGEILLRGKRIRNRLAADAIKN
                     GILMAPEDRKKNGLILKLTVKDNLLMSALGKCVQAGYIRHNLEKQYAEYYIKELNIRG
                     TLNQICSTLSGGNQQKVVLGRVLNADPDLIILDEPTRGIDVKTKSEIHKLMSELAKRG
                     KAILMVSSEMGEILGMSDRIVVLHEGEQMITLKREEANANIIMQYAMGKKEAVVA"
     gene            8220..9206
                     /locus_tag="PK105_06225"
     CDS             8220..9206
                     /locus_tag="PK105_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61343.1"
                     /translation="MGKIAKWPKKERTIRNIGNYGIMIVFIIIVVLLSIAAPTFMTYS
                     NIITILRQVSCIGIATIGVGILIIMNCIDLSIGSMFALSGITAGLMVSTGKEGLALPA
                     IIGIIVGIATGVVVGLFNGIIIAKGKIPPFIVTMGSMSITRGLALILAHGMPVGSFPD
                     SFTYLGTASIDSNNLVPWSVLIFLLVILLMDFLMKRRPLGRYIYAIGGNEEAAMAAGI
                     DTARIKIKAYLLEGGLVGIGATLLASRLKSAAPALGSGYELDAIAGAIIGGVSFLGGI
                     GNVWGMVLGAVMIGVINNGMDLLGVQAFYKQIVKGTIIIIAVLVDRKRAGRA"
BASE COUNT         2814 a         1896 c         2216 g         2471 t
ORIGIN      
        1 atcggcaaaa tggtcgacgg agtctttgtt cccaataaac gctatgagct cgaaggggag
       61 ctgagggaag caaagaaacc cgggcctgca gctagagcga tttcagagcg ccttttctgt
      121 ggtgcgacta ggctcctcga tagcatcgga gaaaagctcg gtattagaga agatcttcga
      181 aaatgctttc ctcaagatta caagaagatt ctctacctcg cgtattttct cctccttgag
      241 gatcatagtc cattaagtag atttcctagg tgggcaatga tacataggca tccttatggt
      301 gcagcgaagg atggagcatg agtaccttgc ctatgatatc agctcgatct cttcttactc
      361 tgaactcata aggtttgtaa agtatgggaa gaataaggag catgatggct taccacagat
      421 caaccttgcc ttactctacg gagagtcctc acgacttccc gtgtactatc ggagcatgcc
      481 agggaatata agcgatgtaa agaccataaa gcagctcatt gatgagctag gctatctctc
      541 tattgagaaa gtaaagctca tgctccaatg cacgctttcg gctgtactgc acttcaagga
      601 cgattgaatg gccttatgaa cagcataagc ctcgattaga aacagtagag agtgacactc
      661 ggaggatgta tttgcatctc tattacgatg atgagagggc tatggaacaa agggctcgct
      721 ttaatttgct ccgagaaggc attagaatac ctgatcacga gactctctac caaaaatatt
      781 acgagctcac ccagactcct gtacgaagag taaagctcaa tcccaagcaa gaggcgatta
      841 ccgaggttga gcgaaactat ggctactttg cccttctgtc caatgatagc aaagaccctc
      901 tcgaagcact caaaatctat cgcaccaagg atcttgtcga gaaagccata ggaaacttaa
      961 aggagaggct ttcttccagg cgagaaggtg tttcctcaga atcgaatctt tcaggaaagc
     1021 tctttgtaca atttgttgca ctcatgtatg tatcatatat agataagctc atgcatgacc
     1081 aaggccttta taagacctat accctcaccc agcttctcga cgaactcgat gtcatcgagt
     1141 gctatagcta tcctggacat atcgagaaga ctggcgaaat gacgaagaaa caaaaggaac
     1201 tctatgaagc tttcggagtg actctccctg cctaggtata aaaaagcgag aattcaggaa
     1261 ataaaacaaa tcaaagggac agtatccgtt ggaagaagta tggcagaacc tatctttgaa
     1321 gcagagctgg caatggtgca attgctataa gagcttgtat gggaaggagc aaagaggatg
     1381 ctctttgaac agtattacat ggcaccaatg acttcgttcg ccatctctta agagctcttc
     1441 aaaagcccaa cttaccgctc cgtctttgtc tagcaatatc caatagaatt tcatctgtat
     1501 acttagcagt gtcgggatgg aaagcattga ttgcaccatt gcgatcggct tcggtaacaa
     1561 tgacgattct ttcgtttgct gctaatccat tgagtatgcc gcgagcagat tcttctggag
     1621 ttattgctga tttgggaggc cctccgagtt catcatatat ggccgttgca acagtacctg
     1681 gaatgaccgt ggaaaagcga atgttctcat cccaatattc gtagcgtagc gagccggtaa
     1741 gccctaccaa ggcagacttg gtggcgctgt acatactttg ataggccatt ggagaaaaag
     1801 ccgctccgga ggcagtattt gcaatatgtc caccaccttg tgcgcgcatt atgggcaata
     1861 ccgcccggat accatacaaa gcgccgaaaa aattgatatc aaaggcaaac ttccaatccg
     1921 catctgttgt ttcgtcgaaa gccttgagca atccgagacc ggcattgttg aaaaggaaat
     1981 cgatttgccc gcctccaaat ttcgccgcct gttggatcat cgcattgata ctttcctgtt
     2041 tggaaacatc ggtttggatt cccagtactt ttccagggta tgcgctctcg agtctcgcgc
     2101 tttctgcttt caatttctcg gcatttatat ctgctagaac gacagccttt gcgccaaatg
     2161 agagaagtag ttcgcacagt gctagaccta ttcctgaagc tccgccggtt actacggcaa
     2221 ctttgttctc ataatactcc ttcatgcttc aaaccctcct tttggattgg gaagataccc
     2281 ggccataaat atttgctata gatattggct acataaattg gcgtcgatag atgctctact
     2341 taaggacacg tccctgagct tccgtaagaa tttcccgacc ttgagtcaat atgacaggct
     2401 tcatgtcgat aatgggcagc tctgccagag cattgattat gtagaattgc atagggttct
     2461 gatcccatgt tgactttgtc catttgactt tcccactgcc taaccaagct gaatttggct
     2521 cgttgtgcat tgggaaaaga ataggttgac tcaaatctgc tcccggcgcg cctactttcg
     2581 gaatgtaacg ccaacctaat gaatttatgt cggtattcat ggcttttacc tcctccgcat
     2641 ccaatggttg atgcctaatc atttccagtt ccaaaaaggt gctgccttcg aaactgaggc
     2701 gagtacggta cgtatccgca aggatatgca ggtcttcaat gtcagcatag atcttcggga
     2761 ctcccatcat gtttcctgtc aaaatcggcg tggtcttatt ctcccaaacc acgagagaaa
     2821 acgacccttc caatcggtcg cgttggccgt tgaaacgcac cggtgccgca acggaaacca
     2881 gatcgtagta cgacccggcc atccattcga cctgtcggca ttgctgaaat tcaatactca
     2941 actccggttt tgtgagctcg aagccttccg gcacataatt agacaattgc tctccgtctg
     3001 ttgtgtagct gaagatcaat gtgaccacgt cgtcatagat agcctttgcg tcggggtcaa
     3061 agtaagagcc gccaaagtga gctggcatct tgtaacattt tccttcttca aacttaaaca
     3121 tgacattcct cctaaaattg gatagtctat ttggtgcctt ccatgttgct gcgcattttg
     3181 tctagcgtga ctaaaatact tagaagatat gaaagaacgc ccccatttgc agcaaggatc
     3241 acgccaatga ccattcctgg cactatgcca gggataagca tcgacatacc gaataggatt
     3301 tgcaagagat tcaaggtcag ctgtgtcgca aagagctttt tgagtatagg tgggataggc
     3361 tcgcgcggtt cagtcttttg cttagaccct ttgggtaaca ttttagtgag cgcaatgatg
     3421 cctcccaaga tgttgagaat accgacgaga attgtcagta ccgatacaag gattccagga
     3481 attatgcatg agatggctcc taaaaccgca aacaagatcc caagcccaat catgggccag
     3541 gtacgaggga aggggccaat tggcgtattc ccgaatgcaa gcatctgaat cgcaaaaatc
     3601 accattagaa ttcccaattg tgcgctgctt gaaaaaggaa gcatgcccaa atttaccgga
     3661 ataagcagca cgccaatgat tatcatgaaa atactagtga acaggatcat tgtctgctca
     3721 atagaaagct tgatgtcgcc aataggattt tttgctgctt ctgggtatgt tctatctatc
     3781 tttccgagca cagtagcgag gtagaatact gctgtgccaa aaaataaaat cacaaccgct
     3841 gtcattggcg ttgtcaacag attctgcttc caaaggagca gcgcgatgac catagacaaa
     3901 atatagaccg cgctgcatgc aaagatcaga tgtcgaaaaa tccccccata tttcacccaa
     3961 cttttgagct tatctttgat aaacaacatt tgtaaaaata gcagcaagcc gcctggacca
     4021 aggcagagaa acaaaagcac gcgggaaaag ctaatcgaaa tcggaataag actggtcacc
     4081 atgccaatta tcgcaatcaa taaccccaca actaacagga gtcttgatct gggcgtgtcg
     4141 ccaaatggag ttctcccaag ggttatcgtc tgcagggcga atatgattaa gagcaatcca
     4201 tataaaccat tctcgtagta ggggagagtt ccgctataaa ctgggaagag catggccccg
     4261 gtgatcagta tgattaccgc agcgataatc agcataacta tttcgaatgg aagctttgcc
     4321 tctctggaaa tcatatgaac catccttctt gctatgcctc taattagggg ttctaatcct
     4381 ggcaggggtt ctaatcctgt cgcctctctt tttattgacc attattcgct tttggtccag
     4441 gctgcttcct taggaatact atttagtgtt atttcagtct gaattcagcg cctacaactg
     4501 cagtgacatc ttttaaccgg cttgaggtat cgttaattcc ataatcatca accatagtcg
     4561 agtatcgagg cgtaatctgg aagacgccca tccgtgcact tttgaccgcg cctatcttag
     4621 cgcctgatga agaagctatg gcttcggctc tctttttggc gtcctctgtg gcgcgctcca
     4681 gtaagctatt ccgcaaatct gccagtttgg tatacatata ttcaagagag gcagagtcca
     4741 gcgcgacgcc ctgggcgata aggtcgccag cacttcgcga tacgcgctcc agtctgtcta
     4801 tgtccgtcga tgtcactcta taactgcagt tggcgaaata ggtaacatgt tgtgtgcgac
     4861 ggccttgctg atcttcgtca aaatagatat tgctgccagc gtttatggca ctagcgacaa
     4921 tctcttcggc tggtatgccc tgcgtggcca agaatgtgtc aatctttggc atggctgttt
     4981 ttaggtctgc atacaccgcc tggaggctat taccgcggat gttgacagaa aagttccaca
     5041 cggaaatgtc cgattctacc ggttcctttg cgcttcccgt gacgctgatt gtgtcctgcg
     5101 ccccgcgcac ctgcacaaaa ccattaacaa caatgatagc catcacgact agtgcgactg
     5161 ccaaaagggc agaagcccaa aacacttgat gaaatttctt atccataatc ttctacccct
     5221 tctttaagca gacccttgca gcaatctcag ttgccaacag attattggct ttagaataac
     5281 attcttaggc aatttcctat atccacttca aaatataaaa ctatttttat tttatcccta
     5341 ttcttctgct tcctgcaatt agctagattg acaattttaa tcttcaattt tgttgacata
     5401 tatgaaatgc tgttgtataa tccgcaataa gtattgataa atgataaagg gaggtaattg
     5461 tctaaatata gctaattact tgcatagtaa ataatattga gtaaggctgt ttgtgttgat
     5521 gtcatataga cgtttctatg ttttgcaatt ggcaagatcc atatagcggg agaagacata
     5581 aggaggtgag gataagcatt tttataaagc tttgataagc gggatctatt ttggatctcg
     5641 atattgatga ggaggcagat agatgaaaag taaaatgaag acaatagcaa ctacattgct
     5701 aatgttagtc ctatgcttgg gaatcgtagg ggcggctcct aaaaaaattg tgataggaat
     5761 cacgatgcaa ggaaatcaga gcggttttat acagtatata accgctggta tgtgggagtg
     5821 gcagaagaac aatgcgccag atgtaacctt aaaggttgtc tttgcggatg atgatgcaac
     5881 gaagcagctg acacaggtag aaacattcgt ggcacagaaa gtcgatgcaa taatcattaa
     5941 tcctgtagac aaagtgcaaa gcgccgcagc cgtggatatt gcagcacaag caaaaattcc
     6001 aatcttgaca gttaatacaa ccacagtgag caagaatgcc acagcccatg taggttcaga
     6061 tgatgtggaa gcgggctatc tacagatgaa tagaataatc aaggtgtgcg gtgaaaaggc
     6121 aagggtagcg tatgtagatg ccgttcttgg tcattctgct caggttgggc gaacagaagg
     6181 ctataaaaaa gcattaagtg aacatcctgg agctactttg gttgtccatg atacaggaaa
     6241 ttggtctgcg gatgattcga tgaagctagt tgagaactgg atacaaagag gattgaaaat
     6301 tgatgcaata gcatgcatgg ccgactgcca attgatagga gtggtgacag cagtaaagaa
     6361 tgcaaatttg ataggaaaga ttgtgcttag tggaatggat tgcgacccac tgataatgca
     6421 atacataaaa gaaggaatag ttgatagttc aatatggcag gatggcgttg ggcaaggggc
     6481 gaatgcagtg agattggcca tagatgccgc aaatggaaag aaagtccagg actttatgct
     6541 gccatttgaa gtatgcacaa aagacaattt tgaccactat atgcaacttg ccaaagaacg
     6601 cgaagaatta gcgaaaaaat atttttaaaa taccggtctg ggaaggaatt gctcggatag
     6661 cactgaaaat gctatccggg caattagtga gagaataaaa tgaggcatga attgaataat
     6721 aatgttgtat tacagatgcg ccatatcgca atggaattcg atggtgtaca ggtgcttgat
     6781 gatgtgaatt taaccctgcg atctggagaa gtacaagcct tagtgggaga gaatggcgct
     6841 ggcaaatcaa cattgatgaa gatattgata ggaatgtacc agcccacttt cggggagatc
     6901 gaatgtaaag gcaatgtcgt gcggtttcgg aatatcaaag attcccaggc acatggcata
     6961 tcgatgatat ttcaagaatt caatcaggta aagcacctta ccgtaatgga aaacatctat
     7021 ctaggacgcg aacctctgac taaagcaggg ctaatagatt acaaagcaat gttcaaacaa
     7081 tccaagcaat tgcttgaaag catgggtgtc gagattagcc ccaatatgta tgtaagagag
     7141 ctcacagtgg caaagcagca gcttgtagag atagcaaaag ccttatcaaa taaggctgaa
     7201 atcctaatca tggacgaacc gacatctgca ctgagcaata aagaaataga gcatttgatg
     7261 aacatgatag gaatgctcaa ggcagcaggg aaatcgataa tattcatctc tcataaattg
     7321 gaggaaatat ataggatttg cgattatgtc acggtcttga gagatggaaa acatatctat
     7381 tccggtttac taaagggctt agacgaaaaa gacttaataa aaatgatggt agatcgtgaa
     7441 attacggaga tgtatccaaa agaaaaggta aatgccaagg atattgtact ggaggttaaa
     7501 ggtctgacgc gtaagaacga atttcgaaat atcagccttg aggtcagaga aggtgaaatt
     7561 ttagggttgg ccggcttgat gggagctgga aggaccgaag taatagagac gattatgggg
     7621 attcggaagg ccgatagtgg agaaatctta cttagaggaa aaagaattcg gaatagatta
     7681 gctgccgatg cgataaaaaa tggcatcctt atggcaccgg aagatcggaa aaagaatgga
     7741 ttaattctta agctgaccgt aaaggacaat ctactgatgt cggctctcgg aaagtgtgta
     7801 caagcaggct atatcagaca taatctcgaa aagcagtatg ctgaatacta tattaaagaa
     7861 ctcaacatta gaggaacact gaatcaaata tgttcgacgc tttcgggagg caatcaacag
     7921 aaagtagtgc taggtcgagt tctgaatgca gatcctgatc taataattct ggatgagccc
     7981 acaagaggga tagatgtaaa gacaaaaagc gagatacaca aattgatgtc ggagctagca
     8041 aagagaggga aagccatcct tatggtctcc tcggaaatgg gcgaaatatt gggaatgagc
     8101 gatcgaatag ttgtattgca tgaaggagag caaatgatca cactaaaacg agaagaagca
     8161 aatgccaata tcattatgca atatgcgatg ggtaaaaaag aagcagtggt ggcataaaaa
     8221 tgggcaaaat agcaaaatgg ccaaaaaaag agcgaacaat acgcaacatt ggcaactacg
     8281 ggataatgat agtattcatt atcatagtgg tcttgttgtc catagcagca ccgaccttca
     8341 tgacatatag caacataata acgatactca ggcaggtttc atgcatagga atagctacca
     8401 ttggagtcgg aattttaatc atcatgaatt gcattgatct ttcgattgga tcgatgtttg
     8461 cattatctgg cataacagcg ggcctcatgg tatctacggg taaggaagga ttggctttac
     8521 cagctataat tggaataatt gtcggaatcg cgacaggagt tgtggttggg ctttttaatg
     8581 gaataataat tgcaaaaggt aaaatacctc cctttatagt gacaatgggc tcgatgtcga
     8641 taactagagg tttggcgctg atattggctc acggaatgcc tgtaggaagc tttcctgata
     8701 gttttacata tcttggaact gcatcgatcg attcaaataa tctggttcct tggtcggttt
     8761 tgatatttct tttggtgatt ctcttgatgg attttttaat gaaacgccga cctctaggac
     8821 ggtatatata tgcaataggc gggaatgaag aagcggcaat ggctgctgga attgatactg
     8881 caagaataaa aataaaggca tacctcttag aaggtgggct tgtgggcata ggagcaactc
     8941 tattggcttc acgtcttaag tcagcagcgc ctgcattagg aagtggatat gaactcgatg
     9001 caattgccgg tgcaattatt ggcggagtga gttttttagg gggcattggc aatgtatggg
     9061 gaatggtgct tggagccgtt atgataggcg taatcaataa tggtatggat ctcttgggtg
     9121 tgcaggcttt ctataagcaa atcgtcaagg gaacgattat cattattgca gtgcttgttg
     9181 ataggaaaag agctggtagg gcatagagtt ggtcttgaat aagcgctcat gcatcgaagt
     9241 attcctaagg gaggctgtga gcaaataggg tctattgaca gctatttctg tatagcagat
     9301 ataatgcaac caggttgtac gatatgcaac caaaaggaag tagaataaat ctatggctga
     9361 aatacaatcg ttggctcgtg gcttaaagat cctagaa
//
LOCUS       DAOOWY010000070         9259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_7402_length_9259_cov_6.906997, whole genome shotgun sequence.
ACCESSION   DAOOWY010000070 DAOOWY010000000
VERSION     DAOOWY010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9259
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7402_length_9259_cov_6.906997"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..966)
                     /locus_tag="PK105_06230"
     CDS             complement(313..966)
                     /locus_tag="PK105_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61344.1"
                     /translation="MNNFLFFAIFALGVVATIQYFLGVKKNRWMGKNLSSQAEGILKP
                     KDTDYVNIGGAIGYNFAYKLKEPWKNAKGTFTLFPRHSLLYMPISLVVGNCDRFYMNI
                     FTDKKLVGEGHIIEKRHSRRAKIEGLEEMHKEEIQRAGKTFVLCWRSINLRDKLIQTL
                     DAMPDASSLTHFCCYSENKTFFLYLKPKKGQISDNLKAFLVQCPTYFRQENPRESNK"
     gene            complement(959..1891)
                     /locus_tag="PK105_06235"
     CDS             complement(959..1891)
                     /locus_tag="PK105_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOU61345.1"
                     /translation="MRDNIFFLGKGGVGKTTVSSAFAMALARRGSRVLAVSLDPAHNL
                     GDALQTKLNGEPKKVEPMLDALEVDLAYWVDRYLEDSKEELKANYSYNSALNLDSFFG
                     ILKYSPGTEEYAVLWAIEDIRRRLAPQYDFVVFDTPPTALTLRFLSMPAISGLWVAEL
                     TKLRERILRDRQTIVRLNPQSPVAESCVDKEDDKVYGKLKNIRTRLTSLQDLFAKRSY
                     MSIIVNPDELSVSEAIRIKDELSQLGIMLSAVCLNKKGLSRAEWTLSPEFGQLPLLIC
                     DFAPNGISTREDLIHIGIEPFIDLFLGKRYVKNE"
     gene            complement(1901..2182)
                     /locus_tag="PK105_06240"
     CDS             complement(1901..2182)
                     /locus_tag="PK105_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61346.1"
                     /translation="MPKRFGHIIKNIFKTFAQVNREKATGMLDFELKELENIFALLIL
                     GGFVGLPSPPSPIAIELLPYMERELIVLLSRSDLSQDPLGVLASMLEID"
     gene            complement(2443..4137)
                     /locus_tag="PK105_06245"
     CDS             complement(2443..4137)
                     /locus_tag="PK105_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOU61347.1"
                     /translation="MTTLIALVALAIYLIFYFAYGRVIRDKLLKSKEAPEAPSKRLED
                     GVDYVPTNKYVLFGHHFASIAGAGPITGPAMAVAWGWLPGLLWIWLGNILLGAIHDYL
                     ALTASVRYDGRSVQFVAQDLMGKKAGTTFGWFTLFLCVLVVAAFGDIVAGQFAADGRV
                     FFSFVFFCVAAVIAGFAMYRSKLGLGWGSVIGLILIILAFWLGDMLPVKMGKEIYFLI
                     IFVYIVLASTLPVNLLLQPRDYLSSFFLYFGLLVGGIAALISAKPLDSIPAFTSFSAK
                     LIGPAGALQPSPFWPTVPLIIACGALSGFHSLVASGTSAKQLKEENDALFVGYGAMLV
                     EGFLATLVIISIASFGAAALGDKLMATPALGRFVNSFALMVSTNLPFLPMSFMTLFTA
                     VWVSTFALTTLDTTNRLGRYIVQEMAEPLKEKNPSTYKILSNKWVASVVIAFLGIFLA
                     WSGGYNTMWPAFSGANQLLASVVMLTMAIWVKKKLNPKYVMSVLIPAAFLWITVTVAL
                     IWYEIVIVPVFFADATKTMNIITGVIVGVINLLMLILNFMMINSFLKNYKLKPETA"
     gene            4360..5478
                     /locus_tag="PK105_06250"
     CDS             4360..5478
                     /locus_tag="PK105_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOU61348.1"
                     /translation="MEDQRLRRLAQLLVNYSTRVQNGDKVLIENSNLEPDFTRLLIEE
                     VHRAGALAFISLRDRRIERTLFMDAPDEQFELQAEFERARMDKMDVYIGFTSVRNSFA
                     WQDLPASKIELYNSHVWKKVHIDRRIPHTRWVVLRYPSAAMAQNAGMSEDAFEKFYFD
                     VCTMDYSRMSRAMDPLVELLQKSDRVKILGHDTDLEFSVKGLPAIKCDGTMNIPDGEV
                     YTAPVRDSVNGILSYNTPNEKDGFKFENIVFEFKDGKIVRARANDTERINKILDIDEG
                     ARYLGEFSFGINPYITRPILDTLFDEKIAGSLHITPGNSYDDCFNGNRSALHWDIVLL
                     QDEAAGGGEVWIDGKLIRKDGLFTLPELRGLNPEALKG"
     gene            complement(5494..5724)
                     /locus_tag="PK105_06255"
     CDS             complement(5494..5724)
                     /locus_tag="PK105_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOU61349.1"
                     /translation="MADKSNRLPQNAKGKWYVDASCIGCGLCSVTAPDIFILGDDGQA
                     YVARQPQTEEENELAIQAMNDCSAQSIGDDGE"
     gene            complement(5897..6316)
                     /locus_tag="PK105_06260"
     CDS             complement(5897..6316)
                     /locus_tag="PK105_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU61350.1"
                     /translation="MNNLVLYDKSPFDLIESLFNNDDWLMPSFRTPAIDVYEEGDKYT
                     IEADLPGLTEKDIKLEVRNGQLILSTAKREKSEDKSKKGRWIRRERREYQFQRSFTLP
                     EEVDPEKIEAHFKNGVLQVVLPKKPEAAPKSIEVKIN"
     gene            6754..7749
                     /locus_tag="PK105_06265"
     CDS             6754..7749
                     /locus_tag="PK105_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61351.1"
                     /translation="MKRLMIAGLVLILSLTVAFGQQRFGVYTTLDEPLAKALFDEYQK
                     ETGIMLNWQRLSGGEVEARLEAEKANPQASIWVGGVGLNHISAKLKGLTAPYKSKMLE
                     NTPLQFRDPENYWVGLYIGPICFVTNNKVAKEQGIKPPTSWADLLKPEYKGKIRVANP
                     TTSGTAYNVITTLRYVFGGDEEKVFDYLNKLDKNIDQYTKSGAAPGKSVAIGEIPVAI
                     GYAHDNVKLKVEGADVTITFPSEGTGYEIASMSIVKGGPDPVNAKKLYDWVLSAKAQE
                     IIASWYVIPVSKIAKKNPQAFSLSEIKTVDQDVMWDAANKDRLLERWVKEIGSKR"
     gene            7888..>9259
                     /locus_tag="PK105_06270"
     CDS             7888..>9259
                     /locus_tag="PK105_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU61352.1"
                     /translation="MFTKKKIIMLSVVCLIFALGEYWIFSTIKNTFEKNTLKEMKALV
                     KAMTSFAPSNNEALSDWIEELGAIYSDNRFFVIKGVPGEEGSEELGGDDALLELWQLP
                     EYADTASSAAESVSYLEIYKWPKIVHFKGIPYTLMVAPIPDAENSEARATLLAAIDST
                     GYVSFTRLVDALALIFFALFAIGFGIATFSRDPISGYAIVVLAAIVLAFVAYPLLEAV
                     RLTLIKDGRFSFNVWKNILSNRQYLQALWGSIQLGIVTATISTFIGFVYAFAVARTSI
                     KGKKLITTLATMPVISPPFSLTLSIILLFGKNGIVTRQWLGLQNVNIYGLWGLALAQT
                     ISMFPIAFMTMQGVLEAIDSTLEDASLDLNASRWYTFSHVTLPLAAPGLLSSWLLVFT
                     NSLADFANPLLLSGSYRVLSVESYIEAIGMNRLNNGAALSLLLLLPTLTAFLAQRRWV
                     NRKSFVV"
BASE COUNT         2496 a         2064 c         2156 g         2543 t
ORIGIN      
        1 gcattgccat cgaggagtac cgatggcaag cttgactaac attgtttttt ctttctcgct
       61 ataggtgact ttctctagaa gcccgaggtt tactattggc tcttcggatt gtggctcttt
      121 tacttgggcc aaggtctcat cgatcttttt tttcatttca gggtccatag gcgcctcctt
      181 ttacttcctt ttgtctactc ttgccttatt tgcttatgca gctggcatat aatacattgc
      241 gggcgctggt tatcgctatc ctcgcactct ttaggaagct tccttagggc tgcacagatc
      301 aagcatagca tcctatttat ttgattcgcg aggattttcc tggcgaaaat aagtgggaca
      361 ttgcaccaaa aaggctttta gattgtccga tatttggccc tttttgggct tcaagtaaag
      421 aaagaatgtc ttattctctg agtagcagca gaaatgcgtt agcgacgagg catcgggcat
      481 ggcatcgagg gtctggataa gtttgtctct tagatttatc gagcgccagc agagcacaaa
      541 tgtttttcca gcgcgctgga tttcctcttt gtgcatttcc tctaggcctt caattttagc
      601 cctgcgagag tgtctttttt cgatgatatg accttctccc accagctttt tgtctgtaaa
      661 gatattcata taaaagcgat cacagttgcc cacaacaagg gaaatgggca tatataggag
      721 agaatggcga gggaacagcg tgaaagtgcc ctttgcattt ttccatggtt ctttcaactt
      781 ataagcgaaa ttataaccaa tcgccccgcc tatattgaca taatcagtat ctttcggttt
      841 tagaattccc tctgcttgcg atgagaggtt tttccccatc cagcggtttt tctttactcc
      901 aaggaaatac tgaatcgtcg cgacgacccc aagtgcaaat atcgcgaaga ataaaaaatt
      961 attcattctt tacatacctc ttgcctagaa aaagatctat gaatggttcg atgccaatat
     1021 gaatcaagtc ttctctggtg ctaatgccat ttggcgcaaa atcacagatt aatagaggca
     1081 attggccaaa ctcgggcgag agtgtccatt ctgccctcga aaggcctttt ttattcaggc
     1141 atacagcaga gagcataatc cccagctgag acaattcgtc tttgattctt atggcttccg
     1201 acaccgaaag ttcatcgggg tttacaatga tgctcatata actccgcttt gcaaacaaat
     1261 cctgcagcga tgtaagtctt gttcttatat tcttgagctt tccatagacc ttatcatctt
     1321 ctttgtccac gcagctctct gcaaccggcg actgaggatt taagcggacg attgtctgtc
     1381 gatcgcgaag aattcgctca cgcagttttg ttagctctgc tacccaaagg cctgaaatag
     1441 ccggcataga aagaaagcga agcgtaagag cagtcggagg cgtatcgaag accacaaaat
     1501 cgtattgagg cgcaagtctg cggcgaatat cttcgattgc ccacagaacc gcatattctt
     1561 ccgtacctgg tgaatatttt agaatgccaa agaaagaatc aagatttagc gctgaattgt
     1621 aagaatagtt ggctttgagt tcttctttgc tgtcttcgag atatctatcc acccaatatg
     1681 ctagatcgac ttctagcgca tctagcatgg gttcgacttt tttgggctct ccattcaatt
     1741 ttgtctgaag agcatctccc aggttatgcg caggatccag cgataccgca aggactctac
     1801 tgcctcttct tgccagagcc atggcaaagg cactggatac cgtcgtcttg cctacgcctc
     1861 cctttccaag gaagaaaata ttatcgcgca cagagactcc ttaatctatt tcgagcatac
     1921 tagccaatac acccagaggg tcctgagata gatccgacct ggagagaagt acaatcagct
     1981 ctctttccat atagggcaat aattctatgg caataggcga tggtggcgaa ggcaggccta
     2041 caaagcctcc aagaataaga agcgcaaaga tattctccag ctccttgagc tcgaaatcga
     2101 gcatcccggt tgccttttct ctgtttacct gagcaaaggt tttgaatata ttcttgatta
     2161 tgtggccaaa gcgttttggc atttttgctt ccttcgggct catctatata agacttctct
     2221 tggctcaact atgattgcct aactatagcg cgctcagagc aagattgaca agcttagagt
     2281 ttctacttct tatcgcatct caactaattt gttcaatgtg ccatagctca tagctcaaaa
     2341 cagaaaaaga gccgaaaagc acatggcttc cggctcttcg tttttgtcat ccgcccataa
     2401 ttgcaatcca acggaccaaa gtcaggcgga aattaccatt tatcaggctg tctctggctt
     2461 cagcttatag ttctttagga atgagttgat catcatgaag ttgagtatga gcattaagag
     2521 gttgatgact cctacaataa cgccagtaat tatgttcata gtctttgtgg catctgcaaa
     2581 gaatacaggg actatgacga tctcatacca gatgagcgct actgtaacag ttatccaaag
     2641 aaaagcagcc ggaatcaata ccgacatgac atatttgggg ttgagcttct tctttaccca
     2701 gattgccatg gtgagcatta caaccgaggc gaggagctgg ttagctccgg agaaggcggg
     2761 ccacatcgta ttgtatccgc cagaccaagc taggaagatc cccaaaaatg cgatcacaac
     2821 cgacgcaacc cacttgttcg agagtatttt ataggtgctt ggattctttt ccttgagggg
     2881 ctcagccatt tcttggacta tgtagcgacc aagtctattt gtcgtatcga gagtcgttag
     2941 agcaaaagta gacacccata ccgccgtaaa gagcgtcatg aaactcatag gcaagaaggg
     3001 gagatttgtg ctgaccatca atgcaaagga attgacaaat cgcccgagtg caggagtggc
     3061 catgagcttg tctccgagtg cggcggctcc aaaagatgcg atagaaatga ttacaagagt
     3121 agcaaggaat ccttcgacta gcattgcgcc atatccaacg aacagcgcat cattctcttc
     3181 tttgagctgc ttcgcgcttg tgccagaggc cacaagagaa tggaagcccg agagtgcgcc
     3241 gcaagcaatg atgagaggaa cagtaggcca gaagggtgaa ggttgaagag cgcccgcggg
     3301 cccaatgagc ttggcgctaa aggaggtgaa ggctggtatc gaatcgagtg gttttgcact
     3361 tatgagcgct gctatacctc ctacaagcag tccgaaatac aggaagaaag aagacaggta
     3421 atcgcgcggc tgtaagagga ggttaactgg caaagttgaa gctaacacga tgtacacaaa
     3481 gatgatgagg aaatatattt ccttgcccat cttgacgggg agcatgtcgc caagccagaa
     3541 ggcaaggatg atcaatataa gtcctatcac ggaaccccag cccagtccaa gcttcgaacg
     3601 gtacatggca aagcctgcga taactgcggc gacgcagaaa aacacgaagg aaaagaagac
     3661 tcgtccatcg gcggcgaact ggcctgctac aatatcgcca aaggctgcca ctaccagcac
     3721 acagaggaat agtgtaaacc agccaaaggt cgtgcccgcc tttttgccca taaggtcctg
     3781 ggcaacgaat tgcaccgagc ggccgtcgta tctgacagat gccgtcagcg caagataatc
     3841 gtgaattgcg cctaatagta tgttccctag ccaaatccat aatagtcctg gcagccatcc
     3901 ccatgccact gccattgctg gccctgtaat tgggcctgcg cctgcaatcg aagcgaagtg
     3961 gtgaccaaac aggacatatt tgttggtggg cacataatcg actccatctt caagtctctt
     4021 agatggagct tctggcgctt ctttgctttt tagtagcttg tcccggatta cccgaccata
     4081 tgcgaagtag aatatcaggt atatcgcgag ggcaaccaag gcaatgagag ttgtcatgca
     4141 gtcctcctat cagaaaacgg atatttcatt ttaactacag tttcttgatg gcgtcaatgg
     4201 ttttttattc tatatttgtg tactattgag atggatggat cgctttttgg atttcttgaa
     4261 tattggttcc aatatatggc gctattcaat aagacgatat tcaagagagt gtatataaat
     4321 tttttgcaaa atcaaactta ttcgcaaagg aagtaatcga tggaagatca aagactcaga
     4381 agactcgcgc agctcttggt aaattattcg accagagtgc aaaatggcga taaggtgctc
     4441 attgaaaact ccaaccttga accggacttt acacgccttc tcatagaaga ggtgcatagg
     4501 gcgggagcac tcgctttcat ttcattgaga gatagacgga tcgaaagaac actctttatg
     4561 gatgcgcctg acgagcagtt cgaactacaa gcggaattcg agcgcgctcg catggacaag
     4621 atggatgtat atatcggttt tacaagcgtt cgaaattcat ttgcatggca agacttaccg
     4681 gccagcaaga tagagctcta caatagccat gtctggaaaa aagtgcacat cgatcggcga
     4741 ataccgcata cccgctgggt tgtgctgcgc tatcctagcg cagcaatggc acagaatgca
     4801 gggatgagcg aagatgcatt cgaaaaattc tatttcgatg tatgtacaat ggactatagc
     4861 agaatgtctc gcgctatgga tccgcttgtg gagcttcttc agaagagcga tagagtcaag
     4921 atattaggcc atgacaccga tctcgaattt tcggtaaaag gtcttcccgc tatcaaatgt
     4981 gatggaacta tgaacattcc agacggagaa gtctataccg cgccggttcg tgattcggta
     5041 aatgggatac tcagctataa tactccaaac gagaaagatg gctttaagtt cgagaatatc
     5101 gttttcgaat tcaaagatgg aaaaattgtc cgggctcgtg ctaacgatac cgaacggatc
     5161 aataagattc ttgatataga cgaaggagct cgttatcttg gcgagttttc ttttggtatc
     5221 aacccttata ttacccgccc aatcctagat acgcttttcg atgaaaaaat cgcaggatct
     5281 ctgcatataa cacccggaaa ctcgtacgac gattgtttta atggcaatcg ctccgccctg
     5341 cactgggata tagtactttt gcaggatgaa gctgcgggcg gcggagaagt atggatcgat
     5401 ggtaaactca tccgaaagga tggactcttt accttacctg agttgcgagg attaaacccc
     5461 gaagctctca agggttagag gagactagag agcttactct ccatcatctc cgatcgattg
     5521 tgcagagcaa tcgttcatgg cctgaattgc cagttcgttc tcctcttccg tctgaggctg
     5581 acgagcaaca taagcctgcc cgtcgtcgcc tagaataaaa atgtctggtg ccgtaacaga
     5641 gcataggcca cagccaatgc aggatgcgtc gacataccat ttgccttttg cgttttgagg
     5701 aaggcgatta gatttatccg ccataatatc tcctttgccg gcaaagccgg ttatgtaaat
     5761 ctagaattcg ttcagcatga taacacaaga aggctgagag ctgaaagccg tgctataaaa
     5821 aaaccgggcc taaagcccgg tttctattaa ggaagattaa gaaaagcaac aaagaagctc
     5881 tgctaagaag cgcagcttag ttgatcttta cctcgatgct cttgggagca gcttcaggct
     5941 tctttggaag cacgacctgc agcacaccat tcttgaagtg ggcttcgatc ttttcgggat
     6001 ctacctcttc aggaagagta aaactgcgct ggaattggta ctctctgcgc tccctgcgga
     6061 tccaccgacc tttcttggat ttatcttcgc tcttctctcg ttttgcggtg gaaagaataa
     6121 gctggccatt ccggacttcc agtttgatat ctttttcggt taggccggga agatcggcct
     6181 cgatggtgta tttatcgcct tcttcatata cgtctatggc tggagtgcgg aagctcggca
     6241 ttagccaatc gtcgttgttg aaaaggctct cgataagatc aaatgggctt ttgtcataca
     6301 ataccagatt gttcatagtc aacctcctac tatttaggat cagcaggtgt cgatccacac
     6361 aggatctcat agcctgtccg gagccccttt tggcgctcat atataatata agcaaaaaat
     6421 gtgccaaatc caaacaaatt gacaattaaa tataattcat tataatataa caaactgcct
     6481 gcgatatccg gacagcaagc ctgctataga tgtagataaa gagctctcat atcggtttca
     6541 ataagacata atcagaagaa cagttcttgg atttatattt ccgattgata catttgtatt
     6601 gatatgcgcc agtagacttg tataataatg atacagattg aatcattgat cttcttagct
     6661 atttgctgaa tcctatttgc cacaatattt tgccttgcga aaatagaagt catggaatac
     6721 actatatttg taaagtactt ttaggaggtt tccatgaaac gtcttatgat cgcagggctt
     6781 gtgctaattc tcagtctgac tgtggcattt ggacagcaga gattcggcgt atatacaaca
     6841 ctggatgagc ctctcgctaa agcgctattt gatgagtatc agaaagaaac gggaataatg
     6901 ctcaattggc agagactttc cggcggtgaa gtagaggcca gacttgaggc agagaaggca
     6961 aatccccagg cttccatatg ggtgggcgga gtaggactca atcacataag cgccaagctc
     7021 aaaggcttga cagctcctta caaatccaag atgctcgaga atactccttt gcagtttcga
     7081 gatccagaga actattgggt agggctttat atagggccca tctgctttgt aaccaacaat
     7141 aaagtagcta aagaacaagg aattaaaccc cccacgtcct gggctgatct gctaaaacct
     7201 gaatacaagg gtaaaatacg cgtggcaaat ccgacaacat ctggtacggc ctacaatgtc
     7261 ataaccacat tgcgttatgt atttgggggg gatgaagaga aggtctttga ctatctgaat
     7321 aagcttgaca agaatatcga ccagtatacc aaatcgggcg ccgcgcctgg aaagagcgtg
     7381 gctatcggag aaattcctgt agctattggc tatgcgcacg acaatgtcaa gctcaaggtt
     7441 gaaggtgcag atgtcactat caccttcccc tccgaaggaa ccggttatga gatagcctct
     7501 atgtcgatag taaaaggcgg acccgacccg gtaaatgcca aaaaactata tgactgggtg
     7561 ctttctgcta aggcgcagga gattattgca tcatggtatg taattcctgt atcaaagatt
     7621 gctaagaaga atcctcaagc cttcagtcta agcgagatca aaaccgttga tcaggatgtg
     7681 atgtgggatg cagcgaataa ggatcggctt ctcgagcgat gggtcaagga gatcggttcc
     7741 aagcgctgat ttttgatctt atttaggatc acattccgaa atgtgaaaaa aattaatgag
     7801 ccggaccctg aggaatgtta tgttcctcaa gggttcggtt cttcgtattc ttggttttga
     7861 tgatgataaa gagaagagga tctccaagtg tttaccaaga aaaagattat aatgctctct
     7921 gtggtatgcc ttatcttcgc tttaggagaa tattggattt tctctacaat taagaatacc
     7981 ttcgaaaaga acaccctaaa agagatgaag gcgctcgtaa aggccatgac ttcttttgcg
     8041 ccttcgaaca atgaagctct ttctgattgg atagaagagc ttggtgccat ctattcggac
     8101 aaccgcttct ttgtgataaa gggagttcct ggcgaggagg gaagtgaaga actcggcgga
     8161 gatgacgcgc tcttggagtt atggcagctt ccggaatatg cagatacggc gagcagcgct
     8221 gcggaatcgg tctcttacct tgaaatctac aaatggccga aaatcgtcca ttttaagggg
     8281 atcccttaca cgcttatggt cgctcccata cccgatgcag agaatagcga agcgcgggct
     8341 actcttcttg ctgccattga ctctactggt tatgtttctt ttaccaggct tgtagatgct
     8401 cttgccttga ttttctttgc tttgtttgcc attggctttg gcattgcgac attctcgcgt
     8461 gatccgatca gcggttatgc aatagtggta ttagccgcaa tagtgcttgc gttcgtcgct
     8521 tatccgcttt tggaggccgt gcgtcttacc ttgataaagg atggcagatt ctctttcaat
     8581 gtatggaaga atattctttc caatcgccag tatttgcagg cgctttgggg ttcgatccag
     8641 cttggaattg tgaccgcgac gatttcaact ttcataggct ttgtctatgc ttttgcggta
     8701 gcgcgcacaa gcataaaagg caaaaaattg ataaccactc ttgcgacaat gcctgtcata
     8761 tcgccaccat tctctctaac tttgtctatc atcctgttat ttggcaagaa tggtatcgtt
     8821 accaggcaat ggcttggcct tcaaaatgtc aatatatatg gtttgtgggg attggcttta
     8881 gcgcagacaa tcagcatgtt tcctatcgca tttatgacta tgcaaggagt cctggaggct
     8941 attgactcca cattggagga cgcttcgctc gatctcaatg catccagatg gtataccttc
     9001 agccatgtga cattgccgct tgccgccccc ggccttcttt ccagctggct gcttgtgttc
     9061 acaaattccc ttgcggattt tgcaaatccc cttctgctat ctggctccta tagggtgctg
     9121 tctgtagagt catatattga agctataggt atgaataggt tgaataatgg cgcggctctg
     9181 tctcttcttc tgctcttacc cactctgacg gcatttctgg cgcagcgccg ctgggtgaat
     9241 cgcaagtctt ttgtggttg
//
LOCUS       DAOOWY010000071         9190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_7486_length_9190_cov_13.576136, whole genome shotgun sequence.
ACCESSION   DAOOWY010000071 DAOOWY010000000
VERSION     DAOOWY010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7486_length_9190_cov_13.576136"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1096)
                     /locus_tag="PK105_06275"
     CDS             complement(83..1096)
                     /locus_tag="PK105_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOU61353.1"
                     /translation="MKNLSLQKSEKSSPQKDNERIASRQAHSPQKVSWQKPSYDPAIS
                     CFLEEIYAECNRSERLELDPLAIVRRYEKPEDMEVAGLLCSTLAFGSVELIMQACEKA
                     LKPLGEHPAKTLREMRHNEIEQAWAFFQYRFCFPKDMIALMSAIHEALDSYGSLEALF
                     LKCDTISRQNTGKDPGDSVANEQKTHKGKTETDWQENILAASSTFVLKLHELALKAQP
                     EGLRKNLLPDPCKGSAAKRLFLFLRWMIRHDEIDPGCWNSISPARLIVPMDTHMIRTC
                     TERLRFLPRRKSAGTMASLRDALSVTEAFRLYAPEDPVKYDFALTRPGIDPRPGDERF
                     SCL"
     gene            complement(1144..3138)
                     /locus_tag="PK105_06280"
     CDS             complement(1144..3138)
                     /locus_tag="PK105_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU61354.1"
                     /translation="MKRNSIFLLAIAVFSVLSFLAPSWSYAQQTLPKTDVEQLKKIAS
                     EATDIETLIVLATPRSLSQARSRTKASPYLSEPNKAALDAIAKSIGLILYGPKNAPFE
                     IPVLAKGADQKYITCLAGLSDASAGKSPKIFENGSCSLMGELISALPFFRDSRNEIRA
                     GALAAISRLENLGGISTVPPLVRGQIALEAKQYTEAASQFQKALELDALSEKPACGLA
                     RAWIALGEPGKAKQVLDPIISIALAESAKEISAKDGYNEDPSSNKVASSGGGYIETTE
                     AEQNILSADIKSLYGLALYHLNNILDAEPWLKAALKEDASRTDLLVPLAHAALQRRDY
                     ASAWKNLEGASKIASKDRNWLILKSQYALENSRQVDAERFARTAVSQYPQDPVALAQL
                     VRAIQESTDAQRHKEATDLAKKVLDITKNEPPNLTPLEQAWRKQAEDKAFQFLVMENY
                     NNQDWIMAAKYLQEAGGVPLDKEMVATILRKSGNVQEALQFALSWYIQEPTSEKAVEA
                     YLRSLAMATGATLASAAPTRDVPTAILPGMSGLEMRQEEGANLALLDIVLKFIAAPFS
                     KELKSFLYYMSATLQSDENKAIDQLKDALAERADNVEALVSLASIYLNRYNRQSDKSD
                     TINRDKAVLYLEQAKSLRPNDDEIKARINQLEAELGYSSR"
     gene            3284..4447
                     /locus_tag="PK105_06285"
     CDS             3284..4447
                     /locus_tag="PK105_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU61355.1"
                     /translation="MPMIPLTNACPEKTMHGNVSRMKSARIGAFAAPVLISILIVFCL
                     GLWGLQISGIGFSNLGEFPISNRSLRRELRCALEIFYSPQLKNSGYYSIQRMLAAKRI
                     GTILIKDGQKASAARFLSSLADEHDDYEAWYLFAAGAVYESMDSDPIARILYERILKN
                     LPDMMIDGQSIHRISLISLLEGDSPPYKKAHWYGEFIRRFPEADEVGPYHFLLAKEYE
                     KLGLWPQAIEEYRQFLPFFGVSVPGYPDAHDYARKMVEFNDSPKDWTYADLNDLVSRI
                     RKAIAKRSASELKKYMSKAGFFAMSWYKEGGEESNSNVLFNFSNFMTRGPIYVSPAFD
                     PSFNDSEALLRTSGWTGYIPVWYLCFRKVNFPADPNVHGNWEWAGIYFGEKMQ"
     gene            4444..5859
                     /locus_tag="PK105_06290"
     CDS             4444..5859
                     /locus_tag="PK105_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU61356.1"
                     /translation="MRKIKSIGILCMLYAALSFASAKEIEGANSFQEADEYIVGVSSI
                     LEPPEELESTPIEETQESLDFSQFLPEEPSIEELSIEERAKSLQYDIPMPWGHELFEK
                     YRQYYLSNQGRKILLSTMEKAVPFMDYIQSKVTEYGVPQELAFLPVIESSFSPFAVSK
                     SGATGIWQFMKNSINGYGLTITEWVDERRDFMKSTDAAMKKLMDNYKELKDWPLAIAA
                     YNAGLGSLRRAVNTAGPGCDFWTIYNKGLVSRQALDYVPKFLAVASILRYPDLYGIEL
                     PSSGIKWETISLEQQVDIKLLATKAEIPLDTLKLGNAELHYTITPPENNHLLKIPADK
                     SDQVKTILADSNAPLVRYEIYKVKQGDTLTAISKRYGISISMITKVNPGINPDKIRIG
                     QTLMIPLSIQPLQSSGSSSNIANSGANIQTSIHTVQKGDTLYALARRYGTTAKDLANL
                     NGISLNSILRIGQRLKIPVRL"
     gene            complement(5864..7093)
                     /locus_tag="PK105_06295"
     CDS             complement(5864..7093)
                     /locus_tag="PK105_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOU61357.1"
                     /translation="MTELARAFEFLEAQERDIIQLESLLTAYPALAPESEGIGEIEKA
                     SALEAWLKAQGISNIEHFDAPDPRVPSKLRPNLVATIPGRRVEGPLWIMSHLDVVPEG
                     ELSSWESDPWKVVVKDGKLYGRGVEDNQQGIVSSVFAALAFVKQGIIPERTIKLLFIA
                     DEEVGSKFGIQYLLENHSLFGKDDIIVVPDGGSADGTEIEVAEKNICWLKIRTKGKQT
                     HAAMPDKGANALLAACDLALRVHRLEKSTFTARDPLFFPDRTTINPTKKEANVPNINT
                     IPGEDVFYFDMRILPVYPVSRMLEEVMKQAHAIEAEYNVKIELEVIQSNESKATPADA
                     PVVGMLAEAVKQVYGVEARPIGIGGGTVGAYLRNAGLDCVVWSKMNETAHQPNENADI
                     ANIIGDAKVFAALAMAP"
     gene            complement(7140..8114)
                     /gene="galE"
                     /locus_tag="PK105_06300"
     CDS             complement(7140..8114)
                     /gene="galE"
                     /locus_tag="PK105_06300"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOU61358.1"
                     /translation="MKILVIGGAGYIGSHVARMLADNGNKVSIADNLSTGTEENLFSD
                     YDFYKGDILDYPWLVSIMKHGFDAIVHLAAAKAAGESMIKPEKYATQNITGTINILNA
                     ACQAGISKIVFSSSAAIYGEPKYLPIDEAHSKEPTNFYGFTKLEIERLLEWYDKLRGI
                     KFAALRYFNAAGYDIEGRVRGLEKNPANLVPVIMEVAAGMRSKLEIFGTDYPTPDGTG
                     VRDYIHVTDLAQAHCKALNHLADARQSFAVNLGSEKGLSVLEILKAARRITGRPIPAS
                     IVERRPGDPAKLIASSCAAKQLLGWEAKYSDVDTIIATSWRVYEKHLA"
     gene            8244..9104
                     /gene="nadE"
                     /locus_tag="PK105_06305"
     CDS             8244..9104
                     /gene="nadE"
                     /locus_tag="PK105_06305"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOU61359.1"
                     /translation="MPAEINPSQEKERIVEFIRTQIAKYEFEGAVIGISGGIDSAVVG
                     KLLTEALGRERVFALILPERDSSKRTVSDSLLVCRYLGIRYSVKSISPLVRRLGIYRT
                     KPPAFIFPRKIQEDYVRAQWQKTSSPYINDLKSAGGQSNRRNLAYYRTKNRLRMTILY
                     YEAEQRGYAVAGTINRTEFLIGFYVKWGDDSSDIEPIMHLYKTQVFELAREIGLPQRI
                     IEKEPSPDLAPGITDEFAIGLSYTDLDRILKKNELTLPLSDEDPELVEKVQKILAAVP
                     YRKIRNLSLL"
BASE COUNT         2347 a         2193 c         2101 g         2549 t
ORIGIN      
        1 agaggctgca attggcacag gaggccgaaa ttggcgcatt gttgatatgt ttttgttttg
       61 tagcttggct catcgattgg gctcatagac aagagaagcg ctcatcgccg gggcgcggat
      121 ctatacccgg acgtgtcaag gcaaagtcat atttgaccgg atcttcgggt gcataaagtc
      181 gaaaagcctc ggtaacgctt agcgcgtctc gaaggctcgc catggtccca gcgctttttc
      241 tccgcggcaa gaatctaagc cgctctgtgc aagtgcggat catgtgcgta tccattggaa
      301 ctatgaggcg tgcgggacta atgctattcc agcatcctgg atcgatttca tcgtgcctta
      361 tcatccagcg cagaaacaaa aaaagcctct ttgccgctga ccctttgcat ggatcgggca
      421 gaagattctt gcgcaagcct tcgggctgag ctttgagcgc cagctcatgc aattttagaa
      481 caaaagttga tgaggcggcc aaaatgtttt cctgccaatc tgtctcggtc ttgcctttgt
      541 gggttttttg ctcatttgca accgaatcgc cagggtcttt ccctgtgttt tgtcgcgata
      601 ttgtatcgca ttttagaaag agcgcttcca ggcttccata gctatccaat gcctcatgaa
      661 tggccgacat gagggcgatc atatcctttg gaaaacagaa acgatactga aaaaaggccc
      721 acgcttgctc tatctcattg tgtctcatct cgcgcagagt ctttgctgga tgctctccca
      781 gtggcttgag tgctttttcg caggcttgca tgataagctc taccgatcca aatgctagcg
      841 tggaacataa gagtccagcc acttccatat cttccggctt ctcatagcgt ctcacaatgg
      901 caagcggatc gagctcaagg cgctcggatc gattgcattc cgcatatatt tcttcgagaa
      961 aacatgaaat tgctgggtca taggagggct tttgccagct gactttttgg gggctatgtg
     1021 cttgccggct agctattctt tcgttgtcct tttgtgggct ggacttttcg gatttttgta
     1081 gagagaggtt tttcatgatt ttgttttcga cattgtcgcc atatcgatgc tctagctctg
     1141 tctctatcta gacgaataac cgagctctgc ttctagctga tttatgcggg ctttaatctc
     1201 atcatcgttc ggacgcagag atttcgcttg ttcgaggtat agaacagcct tatctcgatt
     1261 tattgtatcg gacttgtcgg attgtcgatt atagcgattc aagtatattg aagcgagcga
     1321 gacaagtgct tccacattgt cagctctttc agcgagcgca tcttttagct gatctatagc
     1381 tttgttttca tcgctctgaa gagtggcaga catataataa aggaaagatt ttagctcttt
     1441 tgaaaaaggc gcggctatga atttcaaaac tatatcgagc agagccaaat tcgcgccttc
     1501 ttcttgtctc atctcaagcc cggacatccc cggcaaaata gccgtaggaa cgtctctggt
     1561 cggtgcggcc gaggctagag tcgcccctgt cgccatagca aggctgcgca aataggcctc
     1621 aacggctttt tcggaagtag gttcttgtat ataccagctc aaagcgaact gtaacgcttc
     1681 ctggacattt cctgactttc tcagaatagt ggctaccatc tccttatcga ggggaacacc
     1741 ccccgcttcc tggagatatt tcgctgccat aatccaatcc tgattattat agttctccat
     1801 tacgagaaac tggaaggcct tgtcctccgc ttgtttcctc catgcttgct caagcggtgt
     1861 tagattagga ggttcattct ttgtaatatc cagcactttt tttgccaaat cggtggcttc
     1921 cttatgtcgc tgcgcatcgg tcgactcctg aatggctcta acaagctgag caagcgcaac
     1981 aggatcctgg gggtactgac tcactgcggt cctggcaaag cgctcggcat cgacttgacg
     2041 cgaattctcc aaagcatatt ggcttttgag gatgagccag tttctgtcct ttgaagcaat
     2101 cttggaagca ccttccagat tcttccaagc cgaggcatag tctcgccttt gcaatgcggc
     2161 atgggctagg ggaacaagaa gatccgtcct ggatgcatct tccttgagtg cggctttgag
     2221 ccagggttcg gcatcgagta tgttattcag atgatataat gcaagtccat ataatgattt
     2281 aatatcggcg ctcaggatat tttgctctgc ttctgttgtt tctatatatc ctcctccgct
     2341 ggaggccact ttgtttgaag aaggatcttc gttatatcca tctttggccg atatttcttt
     2401 agcggattca gccagagcaa tcgatattat gggatcgaga acctgctttg cttttccagg
     2461 ctctcccagc gcgatccatg cacgcgcaag gccacaggct ggtttctctg agagcgcatc
     2521 gagttctagc gctttttgga attgactggc agcttcggtg tattgctttg cttcgagagc
     2581 tatctgacct ctcacaagtg gcggaacggt cgaaatacca cccagattct cgagtctgga
     2641 aatggccgca agcgctcccg ctctaatttc gttgcgagaa tcccgaaaga atggtaaagc
     2701 agagatgagc tcgcccatca gcgaacaact tccattctca aaaatctttg gactctttcc
     2761 tgctgaagcg tcggatagtc cggccaaaca tgtgatatac ttttgatcgg ctccttttgc
     2821 taacacagga atctcgaaag gcgcattttt tggcccatac aaaatgaggc cgatgctctt
     2881 tgcaattgca tccaatgctg ctttgtttgg ttccgataga taaggggaag ccttggtcct
     2941 agagcgcgcc tgagataagg atcgaggagt cgcaagcaca ataagtgtct cgatatctgt
     3001 ggcctcactt gcgattttct taagctgttc tacatcggtc tttggcagag tttgctgtgc
     3061 atagctccaa gaaggcgcta aaaaggacaa tactgaaaac actgctatcg caaggagaaa
     3121 tatagagttg cgtttcatcc aatatctata tcggcacttc atggagaatt attattctag
     3181 cctaaatgat ctcgcactgc aagaaaaaaa atgacgttga tcgatcattt ggtgaattta
     3241 tccgctttat ccgcgcggta tccagaatgg cttgatccat tttgtgccta tgattcctct
     3301 gaccaacgct tgcccagaaa aaacgatgca tggtaatgta tcaagaatga aaagtgccag
     3361 gataggtgca ttcgcggctc cggtcctcat ttcgattttg atcgtctttt gcttggggtt
     3421 atggggtctt cagatttcgg ggatcggttt ttccaatttg ggcgaatttc ctatatcgaa
     3481 tagatctctt cgaagagagc ttcgctgcgc tcttgaaatc ttttattctc ctcagttgaa
     3541 gaatagtggc tattactcga tccagcgcat gcttgcagcc aaacgaatcg gcaccattct
     3601 gatcaaagat gggcagaaag caagtgctgc aagatttctt tcttctctgg cagatgagca
     3661 tgatgattat gaagcttggt atctctttgc ggcaggtgca gtctacgagt ccatggatag
     3721 cgatcctatt gcccgcattc tatatgagag aattctcaag aatctgcccg atatgatgat
     3781 agatggccag tcgatacatc ggatatctct cataagcttg ctcgagggcg atagtccgcc
     3841 gtataaaaaa gcgcattggt acggagaatt cataagacgc tttcccgagg ccgatgaagt
     3901 gggcccttac cattttcttc ttgcaaagga gtacgaaaaa ttagggcttt ggccgcaagc
     3961 tatcgaagaa tatcggcaat tcttaccttt ttttggtgta tctgttcctg gatatccgga
     4021 cgcccacgat tatgcgcgca aaatggtcga attcaacgat agcccaaagg attggacata
     4081 cgccgatcta aacgatcttg taagccgtat tcgcaaggcg attgcaaagc gctcagcctc
     4141 agagctcaaa aaatacatgt ccaaggcggg attcttcgct atgtcctggt ataaggaagg
     4201 aggagaagaa tccaattcta atgtcctttt taattttagc aactttatga ccaggggccc
     4261 catatatgtt tctcctgcct tcgatccatc tttcaacgac tcggaagctc ttctccgcac
     4321 atcaggatgg acaggctaca ttcccgtgtg gtatctgtgc ttccgaaaag tcaactttcc
     4381 agcagatcct aatgtgcatg gcaactggga atgggctggt atctacttcg gagagaaaat
     4441 gcaatgagga aaatcaagtc tatcggaata ttgtgtatgt tatacgccgc actgtccttt
     4501 gcatctgcaa aagaaatcga aggcgctaat tcttttcaag aagccgatga atatattgta
     4561 ggcgtctcat ctatacttga gccgccggaa gaattagaga gcacccccat cgaagaaaca
     4621 caagagtctc tcgattttag ccaatttctg ccagaggagc cgagcatcga agagctcagc
     4681 atagaagaga gagccaaaag ccttcaatat gatattccca tgccttgggg tcacgagctt
     4741 ttcgagaaat atcgtcagta ttacttgagt aatcagggtc gcaaaattct gctttctacc
     4801 atggaaaagg ccgtgccatt tatggattat attcaatcca aggtaacaga atatggcgtt
     4861 cctcaagagc tggcctttct gcctgttata gaatcctcat tctcgccctt tgcggtatcg
     4921 aaaagcggtg caaccggtat ctggcagttc atgaagaatt ctataaatgg ctatggacta
     4981 acaataaccg agtgggtaga tgagcgccgc gattttatga agagcacgga tgcagcaatg
     5041 aaaaagctca tggataatta caaggaactc aaggactggc cgcttgctat agctgcatat
     5101 aacgcaggct taggatcgtt gaggcgcgct gttaataccg caggaccggg ctgtgatttc
     5161 tggacaatct acaataaggg attggtttca cgtcaggctc tggattatgt gcctaaattt
     5221 ctggccgtcg cttcgattct gcgctatccc gatctctatg gaattgagct accatcgtcc
     5281 ggaattaagt gggaaaccat ttctctggag caacaggtgg atataaagct tcttgctaca
     5341 aaggccgaaa ttccgctcga tactcttaag cttggaaatg ctgaattgca ctatacaatc
     5401 acgccgccag agaacaatca tcttctcaag ataccagccg ataagtcaga tcaagtaaag
     5461 accattctag cggactcaaa cgcacctctt gtgagatacg aaatatacaa ggttaagcaa
     5521 ggcgacaccc ttacagccat ttcaaagcgc tatggtatct ccatttcaat gataacaaag
     5581 gtcaatccag gcatcaatcc agataagata agaatagggc aaaccttaat gattccttta
     5641 tccatacagc cccttcagtc ttctggcagc tcaagcaaca ttgctaattc gggcgcaaat
     5701 atacagactt caattcacac tgtacaaaaa ggtgatactc tctacgctct agcgcgtcga
     5761 tacggaacta cggccaagga tttagctaat ctcaatggca tttcattgaa ttcgattttg
     5821 agaatcggcc agagactaaa gataccggtt cgcctgtgat tctctaagga gccattgcta
     5881 gcgccgcaaa gacctttgca tcgcctatga tattcgcaat atccgcattt tcgttgggtt
     5941 gatgcgcagt ttcgttcatc ttggaccata ctacgcaatc taaaccagca ttgcgcagat
     6001 aagcgcctac cgttccacct cctatgccaa ttggtcttgc ttcgactcca tatacctgct
     6061 ttacagcctc agccagcata cctactacgg gagcatctgc tggcgtagcc ttcgattcat
     6121 tcgattgaat cacttcgagt tcgattttta cattgtattc ggcttctata gcatgcgcct
     6181 gcttcataac ctcttcgagc atcctggaca ccggatagac gggcaaaata cgcatatcga
     6241 aatagaaaac gtcctcgccc ggaattgtgt tgatattggg cacatttgct tcttttttgg
     6301 tgggatttat ggtggtgcga tccgggaaga aaagcggatc tctggcggta aaagtggatt
     6361 tctccagcct gtgcacgcgt agagctagat cacaagcggc gagaagtgca ttcgcgcctt
     6421 tgtccggcat tgcggcatgg gtctgtttcc ccttggtcct tatcttgagc cagcaaatat
     6481 tcttctcggc tacttcgatt tctgttccat cggccgagcc gccgtccgga acgactataa
     6541 tatcatcctt tccaaagaga ctatggttct caagaaggta ttgaatgcca aatttggaac
     6601 cgacctcttc atcggctatg aataagagct ttatcgttct ctccgggata ataccctgct
     6661 ttacgaatgc cagcgccgca aatactgatg atacaatgcc ctgctgattg tcctcgacac
     6721 ccctgccata gagcttgcca tctttgacaa ctaccttcca tgggtcgctc tcccatgagc
     6781 ttagttcacc ctccggtacg acatccaggt gagacatgat ccagagtgga ccttctacac
     6841 gccttccagg gatagtcgcc accagatttg gacgtaactt cgaaggaacg cgcggatcag
     6901 gcgcatcgaa atgctcgata ttcgagattc cttgtgcctt gagccacgct tccagagcag
     6961 aagctttctc aatttctcca attccctctg attcgggagc gagcgctgga taagcagtga
     7021 gaagactctc taattggata atatccctct cttgagcctc cagaaactca aatgctcgag
     7081 caagttctgt catgattttc ccctttcctt tccaggcaga tatgcttcta tcgaattgcc
     7141 tatgccaaat gcttttcgta gacgcgccac gacgtggcta taattgtatc tacatcggaa
     7201 tatttcgcct cccatcccaa gagctgtttt gccgcacagc tcgatgcgat cagtttagca
     7261 ggatctcctg gcctccgctc cactatcgaa gccggaattg gccgacccgt gattcgcctt
     7321 gcggctttaa gaatttccag gacggacagg cctttttccg atcccaggtt tacagcaaag
     7381 ctttgccttg catccgccag atgattcaaa gccttacaat gggcttgggc aagatccgtc
     7441 acatggatat aatctcgcac accagtacca tccggggtag gataatcggt gccgaagatt
     7501 tcgagctttg accgcattcc agcagccact tccataatga cgggcacgag attagctgga
     7561 tttttctcta atcctcgcac tctgccttca atatcgtatc cagccgcatt gaaatagcgc
     7621 agcgctgcga atttgatccc tcttagctta tcataccatt cgagaagacg ctcgatttcg
     7681 agcttagtaa aaccatagaa attagttggt tctttggaat gcgcttcgtc tatcggcagg
     7741 tatttcggct caccatatat ggctgccgag gaagaaaata ctattttgga aatacccgct
     7801 tgacatgcag cattgaggat atttatcgtt ccggtaatgt tttgtgtcgc gtatttttct
     7861 ggcttgatca tggattcgcc tgcagccttt gcggcggcaa gatgtactat tgcgtcgaat
     7921 ccatgcttca ttatcgaaac aagccaggga taatccagaa tatcgccctt atagaaatca
     7981 taatcagaaa agagattctc ttctgtgccg gtagaaaggt tatctgcgat gctgacctta
     8041 ttgccattgt cagcaagcat tcttgcaaca tgactcccaa tgtaacctgc tccaccgatc
     8101 actagtatct tcatgcgatt tctctccttg gcgaatctct acagcattta tgctatattt
     8161 gaatatatcg gcggcgtcaa tatggcaaca tattcttcga tgtatccata agtgtatttt
     8221 cgctgatatc aaagaggccc ttcatgcccg cagagatcaa tccatctcaa gaaaaggaaa
     8281 gaatcgttga attcatcagg acgcagatcg ccaagtatga attcgaagga gccgtgatag
     8341 gcataagcgg agggatcgat tccgctgttg tcggtaaact cttgacggaa gcccttggtc
     8401 gggaaagagt ctttgctctc attttaccgg aacgagattc atcaaagaga acagtctcgg
     8461 attccctgtt ggtctgtaga tatctcggaa ttagatattc tgtaaagagc atttctcccc
     8521 tggttagacg tctcggaatc tataggacaa agcccccagc ttttatattt ccaaggaaaa
     8581 ttcaagagga ctatgttaga gcccaatggc aaaagaccag ctctccttat atcaacgatc
     8641 ttaaaagcgc aggcggacaa tccaacagaa gaaatctggc atactataga accaaaaaca
     8701 gattgagaat gacaattctc tattatgaag cagaacagag aggttatgcg gttgccggaa
     8761 ccatcaacag aacagaattt cttattggat tctatgtcaa gtggggagat gattcgagcg
     8821 atatagaacc aataatgcat ctctataaga cacaggtatt cgagctggca agagagatcg
     8881 gcctcccaca gagaatcatc gaaaaagaac ccagccctga tctagcccct ggcataactg
     8941 atgaatttgc cataggccta tcctataccg atttagacag aattctaaag aaaaacgaac
     9001 tcaccctgcc tctctccgac gaagatccgg aacttgtgga aaaagtacag aagattcttg
     9061 ccgcagtacc ctatcggaaa atcagaaacc tgagcctatt gtgatgggat ggattatcat
     9121 gggagtctgg caatagaatg gatgaccaag ccaatctcga tgcgtcctgt cgttagcatc
     9181 ctgtttgagc
//
LOCUS       DAOOWY010000072         9084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_7612_length_9084_cov_22.452210, whole genome shotgun sequence.
ACCESSION   DAOOWY010000072 DAOOWY010000000
VERSION     DAOOWY010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7612_length_9084_cov_22.452210"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PK105_06310"
     CDS             complement(<1..883)
                     /locus_tag="PK105_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61360.1"
                     /translation="MHLQKRVSVALPHHADKTMLFILIMLCAVLAFNAPNIYAQDYPT
                     QEEPKQLPYIAGFEFHLKGVRETAVLRYLDMQIGRRFSSHSELDSFIAEKQRLLQENR
                     VFDEEATIWYEQRNPSDPEAITIIVSAYATWTSLAVPIPKYTEDDGFFLALRYKDFNF
                     LGMLEPLVFSLDHYFLNGNTTIGAEFGYSPHFINAQWQFALASSVDIGQDLDLAFSDT
                     AISLSTEYPLSEVPEYWKLVPSLSYTYTKSSKDHVGIAGLSLAKPIFLLFPGNIELQA
                     SYKQENDAGSWSYLAIAE"
     gene            complement(896..1990)
                     /locus_tag="PK105_06315"
     CDS             complement(896..1990)
                     /locus_tag="PK105_06315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU61361.1"
                     /translation="MRVLHLVTRSDSGGAQCMIATLAALQSQSGHVVAVASGLEGKGL
                     AWHSLPAAIEKLPVKHLKRNIAPWDDLLALSEIRHICMKWKPDILHVHTSKAAALTRI
                     AINYNRIPIIYTMHGYDQLKIANKKLLFIDKMLSPRTSAIVAVSRYDLGRMRADNYHP
                     ELIANGVNAQSNPPSDAPELVLALRLLENLRRQKSLIGLMIARPSSPKRPDLVREAAR
                     LLKGLCSIVWIGGNARSDDPGAFYALGPIPDAGRLIPFCDFVLLPSDHEGLPMTLLEC
                     LAAGKPFIASNVGGIPELIGNGYFTTAPGFAVNNSPEDFASAICSLAHDTKLRNIMGE
                     NGKRLWERFYTAETMAAEYESLYNHYKQIE"
     gene            complement(1987..3156)
                     /locus_tag="PK105_06320"
     CDS             complement(1987..3156)
                     /locus_tag="PK105_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU61362.1"
                     /translation="MKTRDFLLATCLHFLPFLFALAFSFSGYTNIAIFVILFLSFWYL
                     ILNWQIVIRDRWALCVIGFFLAIAAKDGIMFALGKGSFVAFAKSGSRPVVLAGCSAML
                     YAHSREDIERALMDGFWVTFSYLLIIFILMRFRKIPMIYNINTFGMTGIWAAILTGTN
                     RWQNKIKNSRLERLEALAILISVLILVLQDVSFPGGFEGSRTAVFAMITAIFFVLFSE
                     TKYAMVFARLELALVLVFILIGSMVLIPQLNDLLSNRQYLWQAFLRKGLERPLIGWGF
                     TTEADNIRLVSSYMIDTPFYDAFAKTGLGPHNSFLAMFFENGVIALAGYITLLLARIR
                     AMRPPLTMFDVSLLAYLVFMSLDAMAPGGLTVLGYFLGICILEVPERSQEDKENR"
     gene            complement(3153..5285)
                     /locus_tag="PK105_06325"
     CDS             complement(3153..5285)
                     /locus_tag="PK105_06325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU61363.1"
                     /translation="MNRPLITVLTATWNRASTLPRLKASLEAQTYSNFEWLIVDDGST
                     DETIGLIEQWQLDSSLAIILLRRQNGGKHRSVNDGMRAANGRWIFIVDSDDRLPNRAI
                     ENITIHLPSIEAIPNVAGLSGLKADFSGQIIGESFPAEMKFCDAISLTYRYGIRADKA
                     DVYKKEVLASFPFPEFEGERFLTEAVIWYRIANAGYALLLTNEVLYECEYRGDGLSAH
                     SLELRIANPIGTLLFYKEIVGYDLPYRAKLREASNYMRFCKHYRRSSCFDPRDLEQVS
                     HAYLPPKLRIPASIIGNAAYLYDQLTLARSRNTRNRCNVSASMPFDTKNSIRVLHVIN
                     SLSQGGAERFLASLLPRLKAQHIENMVLVLNAESAIYASVLEEAGIPVYKPGPQGFVS
                     DSNKKRSKKRAQESRLYSPSRVFDVLRAVRCLRPDIVHAHLAPSFHWCALASLFAGKP
                     VYITTEHASKNRRMSIPFFKWIEKACYARYASIICVSPSVAQALSEWLGIRSEKLQTI
                     VSGIDIEQFSNSSQPAPEVVRFLGGRTGIAMTARFVPVKDHPVALEILDRLPERYALI
                     LIGDGPGLSHIQDLAAKMRLEQRCLFLGSREDIPNILAACHLYIQTSQQEGLGLAVAE
                     AMAAGLPVVVRKTSGLFDLVDGAGLFFEARDIEQAVAQIISLENRDLREKMVLAEGER
                     IKYLSIERVAYEYGSHYRKILRGEKETK"
     gene            complement(5282..6538)
                     /locus_tag="PK105_06330"
     CDS             complement(5282..6538)
                     /locus_tag="PK105_06330"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU61364.1"
                     /translation="MIFNRNAIEINEPRALYVRGERVKLIRGGADHVSSSLLCMLRTY
                     FPHSIDELYLDEFCERTKSRRSVLFDALQGWFFPYGRKEAIQFRRILQGYDFVFLDQS
                     VYGRLLSDAKACRPDLKTAVFFHNVECEFYAQRIWHAGKIHNALLLPSILKAERESAR
                     ADLVMALTGKDSKKLRRIYGRSADCIIMPVVADRYNRTVADQAQSRKKPYTFTMLFAG
                     SAFPPNIEGIRWFVRKVLPQVQGELFIVGRGFEKYRARLAAERVHVIGAVEEIDLWYY
                     QSDCVVSPIFWGSGIKVKTAESFMLGKTVVGTKESFVGYDPIAANAIVADEEEQFVKA
                     LRRLHLQTLSGSPKENAQTRAYYLRELSLDAQYAKFSAALDKLFANKNSSSVDKRHQH
                     KHMRKSDLMRISRHKRRLSQPPGSLM"
     gene            complement(6528..>7424)
                     /locus_tag="PK105_06335"
     CDS             complement(6528..>7424)
                     /locus_tag="PK105_06335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU61365.1"
                     /translation="AYLLSLTLPTYLQPETKEIIVVDDCSPDTTREVVESLAADDPRI
                     RYIRTDTNRKQPHAKNLGISQAKGQYIYFGDDDSVLLPGSLRRLISTLHEYDASIVGA
                     CAPYLTRLTNDKDLSSILEEISREMGQSESLYKAEIHKRLISPVSLEAHFNARTPQPV
                     EVPFVHASFLIYRELAEKTRFDEHYSGNCYREETDFLIRTHDAGARIIYEPRAVQANL
                     PRSVAKGGAHDGSSNFLIRKLRYFKSALENNWYFLTKNKGPLRRAFGLNISPLHRQAV
                     FAMIILRVIASYPIRKVIGHDL"
     assembly_gap    7425..7524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7525..8554)
                     /locus_tag="PK105_06340"
     CDS             complement(<7525..8554)
                     /locus_tag="PK105_06340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU61366.1"
                     /translation="MEKTLNFEQNSGKFQSAHGLRSRKIIIISPNGLSDAGGVERVML
                     YAYRALSEHGFRVDIIDQKALSGSNIGRFFSFFKTGPLDFLALSFGLSILANRERRSG
                     SFIINNGYSAFFAKSDILFCHGCIRGARLAQYRVRSEQQLSSLSERRAQIDGHKTSRI
                     TRLIGSLKIFLYGPDEIAEMIAGHRSHIILAVSEHSQREWQRYYRLSSSKFKNLPNSV
                     DTEHFQPDLESHKQQSSRQSRIALRILFIGSLTYLKGLDRLGATIEKARQTAKNWEFI
                     IATPSSFNTEKFASKQNVTIRKGVSFAELPDLYRSCDVMYFPSIYEGFEMVTLEALSS
                     GLPVVGTDV"
     gene            complement(8557..>9084)
                     /locus_tag="PK105_06345"
     CDS             complement(8557..>9084)
                     /locus_tag="PK105_06345"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOU61367.1"
                     /translation="IADDLIKVFAGAQFTPAVTSMRITAWIIPIVAFSNALQMQILIP
                     QQKEKSMLVSFSIGIVVTGLVLCFTVKPFGQAGAAMGMLAGEASVLVAHAYFCGRKAI
                     LTIFARKRLFHYLAGGALCGLVAYCISQLSGVMLIARLILAIAISAFFYFIFLLVVKD
                     DFAILIIKGRHKNTG"
BASE COUNT         2495 a         2135 c         1994 g         2360 t
ORIGIN      
        1 tttctgcaat agctagatat gaccaagaac cggcatcatt ttcttgttta tatgaggcct
       61 gcaactctat gtttccggga aagagcagga aaatcggctt tgccaggctt aagccagcaa
      121 ttccaacatg gtctttggag ctttttgtat aggtataaga tagcgaagga acaagtttcc
      181 aatattcggg tacttcagag agaggatact cagtcgaaag actgattgct gtatcgctga
      241 atgccagatc gaggtcttgg ccgatatcga ccgaacttgc aagcgcaaac tgccattgag
      301 catttatgaa gtgaggcgag tacccaaact ccgcaccgat ggtagtattt ccatttagaa
      361 aataatggtc taaggaaaac actagtggct ccagcatgcc gagaaagttg aaatccttat
      421 agcgtaatgc aaggaaaaaa ccatcgtctt cggtgtactt tggtatgggt acggcaagag
      481 aagtccatgt tgcataggca ctcactataa tggttattgc ttcgggatct gatggatttc
      541 gctgttcata ccagattgtc gcctcttcat cgaagactct attctcttga aggagcctct
      601 gcttttccgc tataaaactg tcgagctccg aatggcttga aaatctccgg cctatttgca
      661 tatcgagata tcgaagaact gccgtctctc tgacaccttt tagatgaaat tcgaatcccg
      721 cgatataggg aagctgctta ggttcctctt gtgtcggata gtcttgtgca taaatgtttg
      781 gagcattaaa agccagtacc gcgcatagca taatcaaaat gaatagcatt gttttatcgg
      841 catgatgagg cagtgccaca gacactcttt tctgcaaatg catagctgtt tcttcctatt
      901 cgatctgctt gtagtgatta tatagcgact catattcagc cgccatcgtc tcggcggtat
      961 agaatctctc ccagagcctt ttcccattct cgcccattat gttgcgcagt ttggtgtcat
     1021 gagcaaggct acagatagcc gaagcgaaat cctctgggct attattcaca gcaaatcctg
     1081 gagctgtcgt gaaataacca ttcccaataa gctcaggaat gccgcctaca ttagaggcga
     1141 taaaaggctt gcctgcagct aaacattcga gaagggtcat gggcagacct tcgtggtccg
     1201 atggcaagag cacaaaatcg caaaaaggaa tgagcctgcc cgcatccgga ataggtccga
     1261 gcgcataaaa ggcgcctgga tcatcggatc gagcattgcc acctatccat actatactgc
     1321 aaagaccctt taacaggcga gcagcctcac ggaccaggtc gggtcgcttg ggagaagagg
     1381 gtctggcgat cattagcccg attagcgatt tttgtcttcg caaattctct agaaggcgta
     1441 gtgctaaaac caattcagga gcatcggatg gcgggttgct ttgagcattt acgccatttg
     1501 ctataagctc tggatgataa ttatcggccc gcatgcgccc aagatcatag cgagaaacgg
     1561 caacgatcgc cgatgtcctt ggagagagca ttttatcgat gaaaagaagc tttttgttgg
     1621 caatcttaag ctggtcgtag ccatgcatcg tatagattat cggaatccga ttataattta
     1681 tggcgattct tgtaagggca gctgctttgc tcgtatgaac atgcaggata tcgggcttcc
     1741 atttcatgca gatatgacga atttctgata gggctaacag atcgtcccat ggcgctatgt
     1801 ttcgctttag gtgcttgacc ggcaattttt ctatagcggc cggaagggaa tgccaggcga
     1861 gccctttccc ttcgaggccg gaagcaactg caacaacatg accggactgg ctctgtaagg
     1921 ctgcaagtgt ggcgatcata cattgggcgc cgcctgaatc ggagcgggta actaaatgca
     1981 gaaccctcat cgattttcct tgtcttcttg agatctttcc ggcacctcga gaatgcatat
     2041 ccccaggaaa tatcccagta ctgtcagccc tcctggagcc atcgcatcaa gggacataaa
     2101 gacaagatag gccaaaaggg aaacatcgaa cattgtcaga ggcggtcgca ttgcccttat
     2161 ccgagcaaga agcaatgtga tgtatcctgc aagagcaata acaccattct cgaagaacat
     2221 tgccagaaag ctattatgag gaccaaggcc tgttttggca aaggcatcat agaacggtgt
     2281 atcgatcata taggaactca caaggcggat attgtccgct tccgtagtaa atccccagcc
     2341 aatcaagggc cgttctaggc cttttctcaa aaatgcttgc caaagatact gtcgatttga
     2401 caatagatcg tttaattgag gaattaatac catcgaaccg ataagaataa agacaagaac
     2461 aagcgcaagt tccaatctgg caaataccat tgcatatttt gtttcggaaa aaagtacgaa
     2521 aaaaatcgcc gtaatcatcg caaaaacagc ggtcctagac ccttcaaatc ctccagggaa
     2581 agacacatct tgaaggacca gtatcagcac cgagataaga attgccagag cctccagtct
     2641 ctccagtctg ctgtttttta tcttgttttg ccatctattt gttcccgtaa gaattgcagc
     2701 ccatatcccc gtcattccaa atgtatttat attgtagatc ataggtattt ttctgaatcg
     2761 cataagaata aaaatgatca acaaataaga gaaagtcacc caaaagccat ccataagagc
     2821 tctttcgatg tcctctcttg aatgagcata tagcattgca ctacagcctg ccagcacaac
     2881 aggccgagac cccgacttag caaatgccac gaagctgcct ttccccaaag caaacataat
     2941 accatctttg gccgcaattg ccaggaaaaa gcctatcaca cataaggccc atcgatcgcg
     3001 aatgacaatt tgccagttca agataaggta ccaaaagctt agaaaaagaa ttacaaaaat
     3061 cgcgatatta gtatatccgc taaagctgaa tgcaagggca aaaagaaatg gcaaaaaatg
     3121 caagcatgtc gctaagagaa aatctctagt cttcattttg tttccttttc tccccgcaga
     3181 atcttgcggt aatgtgatcc atattcgtac gcaacacgtt caatcgaaag atattttatt
     3241 ctctcaccct cggccagcac catcttttct cgcaaatctc ggttctcgag cgaaattatt
     3301 tgcgccacag cctgctcaat atcccgagct tcgaaaaaca agcccgcacc atctacaaga
     3361 tcgaaaagac cgctggtttt tcgcacaacc accggaagcc ccgctgccat tgcctccgca
     3421 accgccaggc caagtccctc ttgctgcgaa gtctgaatat ataaatggca tgcagcgaga
     3481 atattcggaa tatcctcacg cgagccaaga aatagacagc gctgctcgag cctcattttt
     3541 gcggctaaat cctgaatgtg cgatagccct ggaccgtcgc ctatcaatat gagagcatag
     3601 cgctctggca acctgtcaag aatctcgagc gcgacaggat gatcctttac gggtacaaat
     3661 cgcgctgtca tagcgattcc ggttcttcct ccaagaaatc ggactacttc cggcgctggt
     3721 tgcgaactat ttgagaattg ctctatatca ataccactaa ctatggtttg gagcttttcg
     3781 gaacgaatcc caagccattc cgaaagcgct tgagccaccg aaggactcac acagataatc
     3841 gaagcatatc gcgcataaca ggccttctcg atccatttga aaaaaggaat ggacatacgc
     3901 cgattcttac tggcatgttc ggttgttata tagacaggct ttccagcaaa gaggcttgca
     3961 agtgcacacc aatggaacga aggagccaga tgagcgtgga cgatatcggg ccgcaggcaa
     4021 cgaacggctc taagcacatc gaagaccctc gagggcgaat agagtcgcga ttcctgtgct
     4081 ctcttcttag atcttttctt gttggaatcg gaaacaaagc cttgcggccc aggcttataa
     4141 actggaattc ctgcttcttc caacacagaa gcatatatcg cagactctgc attgaggaca
     4201 agaaccatat tctcgatatg ctgcgctttt agccttggaa ggagagatgc aagaaagcgc
     4261 tctgctccgc cctgtgacaa ggagtttata acatgcagta ctcgaataga gttttttgta
     4321 tcaaatggca tgctcgccga gacattacac ctgtttcttg tatttcttga gcgagccagc
     4381 gtgagctggt catagagata ggcggcgtta ccaatgatag atgcgggtat tcgcagtttt
     4441 ggaggtagat aagcatgact tacttgttcg agatctcttg ggtcgaagca ggaagagcgc
     4501 cgataatgct tacaaaaacg catatagttt gacgcttctc tcagttttgc tctataaggc
     4561 aagtcatagc cgactatctc cttgtagaaa agcagagttc ctatcggatt ggcaatgcga
     4621 agctccagac tatgagcgga tagtccatcc cctctgtatt cgcattcata caagacttca
     4681 ttagtcagaa gcaaggcgta acctgcattc gctatacgat accagatcac ggcttcggtc
     4741 agaaatcgtt ctccttcgaa ttctggaaaa ggaaaactcg ccagtacttc ttttttgtac
     4801 acatcagcct tatcggctct aattccatag cgatatgtga ggcttatggc atcgcaaaat
     4861 ttcatttcgg caggaaaaga ctcaccgata atctgtccac taaaatctgc cttaagcccg
     4921 cttaatccgg ccacatttgg aattgcctct atagagggta gatgaattgt tatgttttcg
     4981 attgctctat tgggaagcct atcgtccgaa tcgactataa agatccatct accattagct
     5041 gctcgcatac catcgttgac ggaacgatgc ttgcctccat tctgtcgcct aagaagtatg
     5101 attgcgaggg aactatccag ctgccactgc tcaataaggc cgattgtctc atccgttgaa
     5161 ccatcgtcga cgatgagcca ttcgaaattg gaataggtct gggcctcgag cgaagccttt
     5221 agacgcggta gtgtcgaggc tctattccat gttgcagtca gcacggttat tagaggccta
     5281 ttcacataag gctccctgga ggctgggata gcctgcgttt gtgcctgctt attcgcataa
     5341 gatcgctctt gcgcatatgc ttgtgttggt gtcttttatc tacagaagac gaatttttat
     5401 tggcaaacaa cttatctaag gctgctgaaa actttgcgta ttgtgcatcg agcgagagtt
     5461 ctcgaagata ataagcgcga gtctgtgcat tctctttagg tgagccagac aatgtttgca
     5521 aatgtaatct ccgaagggct tttacgaatt gttcctcttc atctgctact atcgcatttg
     5581 ctgcaatagg atcatatcct acgaaagatt cttttgtccc gacaacggtt tttccgagca
     5641 taaaagactc tgcggttttg actttgattc cagatcccca aaagatcgga gatactacgc
     5701 agtctgactg atagtaccag agatctattt cctccacagc cccaattaca tggacacgct
     5761 ccgccgcaag ccgagcacga tatttctcga agcctcttcc aactatgaaa agctccccct
     5821 gcacttgtgg caatactttg cgcacaaacc atcggatgcc ctcgatatta ggcggaaacg
     5881 ccgatccagc aaataacatg gtaaaagtat agggcttctt tctcgattgc gcttgatcag
     5941 ctactgttct gttgtagcga tccgcgacaa caggcattat tatgcaatct gccgaacgcc
     6001 catagatacg ccggagcttc ttactatctt ttccggtcaa ggccattaca agatctgccc
     6061 ttgccgattc tcgttcggct ttaagaatcg atgggagtag cagagcatta tgaattttgc
     6121 ctgcgtgcca aattcgctgc gcatagaatt cgcattctac attatgaaag aataccgcgg
     6181 tcttaagatc tggccgacag gccttggcat cggaaaggag ccttccgtag accgactgat
     6241 ccagaaagac aaaatcatag ccttgcaaaa tcctgcgaaa ttgtatcgct tcttttcttc
     6301 cataggggaa gaaccagccc tgcaatgcat cgaatagaac agaccgacga ctttttgttc
     6361 gctcacagaa ttcatcgaga tagagctcat ctattgaatg cggaaaatat gtcctgagca
     6421 tgcaaagaag agaactcgac acatgatctg cgcctcctct gattagcttg actctttcgc
     6481 cacgaacata tagagcgcga ggttcattta tttcaatagc atttctatta aagatcatga
     6541 ccgattacct tgcgaatagg gtaagatgcg attacccgta gaattatcat cgcaaacacg
     6601 gcctgccgat gtaaaggaga aatatttagg ccaaaagccc ttcgcaaggg ccctttgttt
     6661 tttgtaagaa aataccagtt gttttccaat gcggatttga agtagcgcaa ttttcttatc
     6721 aagaaattgc tcgagccatc atgcgctccg cccttagcaa ccgaccgcgg aagattggcc
     6781 tgaacagcgc gcggttcgta tataatcctt gcgcctgcgt cgtgcgttcg gattagaaaa
     6841 tccgtctctt ctcgatagca atttccacta taatgctcat caaagcgagt cttttccgcc
     6901 aactcgcgat agatgagaaa agaggcatga acaaaaggca cttcaaccgg ctgcggcgtt
     6961 cttgcattaa agtgagcttc gaggcttacg ggagagatga gtctcttatg gatttcagcc
     7021 ttatacaaac tctctgactg acccatctcc ctagaaatct cctcaagtat agaagaaaga
     7081 tctttatcat tggtcagtct tgtgagatag ggcgcacagg cgccgactat cgaagcatca
     7141 tattcgtgta gagtcgaaat aagtcgtctc aaagagccgg gcaaaagaac cgaatcgtca
     7201 tctccaaaat atatatattg ccctttggct tgtgatatac caaggttttt tgcatggggc
     7261 tgctttcggt ttgtatctgt tctgatatag cggattcttg gatcatccgc tgcaaggctt
     7321 tcaaccacct cacgagtagt atccggggaa cagtcatcca ccacgataat ttccttggtc
     7381 tccggctgca ggtatgtggg aagggtaaga gataggaggt aagcnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnccacat ccgtgcccac taccggcaat cccgaagaga
     7561 gggcttccag cgtgaccatt tcgaagcctt cgtatatcga aggaaaatac atgacatcac
     7621 aagaacgata tagatcgggt aattcagcaa agctcacgcc cttgcgaata gtaacatttt
     7681 gtttagaggc aaacttttct gtattgaagg atgaaggagt tgcgattatg aattcccagt
     7741 tctttgcggt ttgtcgcgct ttttcaatag tcgcgccgag cctgtccaag cctttgagat
     7801 atgtaagcga tccaataaag agaatcctaa gcgcaatccg agattgtctc gaagattgtt
     7861 gtttgtggga ttctagatca ggctggaaat gttctgtatc caccgaattt gggaggtttt
     7921 tgaatttgct ggatgaaagg cgataatagc gctgccactc cctctgcgaa tgttctgata
     7981 ccgctaagat gatatgcgag cgatgcccgg caatcatttc ggcaatctca tcgggtccat
     8041 acaagaaaat cttcaaactt ccgatgagcc ttgtaatcct gctggtttta tgaccatcga
     8101 tctgcgccct gcgctcagat aagctcgata gctgttgttc cgagcgcact ctgtactgtg
     8161 ccaagcgcgc acctcggata catccatggc aaaaaagaat atctgatttt gcaaaaaatg
     8221 cggaatagcc attatttatt atgaaagaac ccgatcgacg ctctctatta gcaagtatgg
     8281 acaatccaaa ggatagtgcc aaaaaatcta atggtcctgt cttgaaaaac gagaagaatc
     8341 gaccgatatt gcttccagac aaggcttttt gatcaattat gtcgactcga aagccatgtt
     8401 ccgaaagcgc acgatatgca tatagcataa ctcgctctac tcctcccgcg tcggagagcc
     8461 catttggaga gatgattatt atctttcgag acctaagtcc gtgagcggat tgaaattttc
     8521 cactattttg ctcaaagttc aaggtctttt ccatatctat ccagtattct tatgtcgacc
     8581 ttttatgatc aggatcgcga aatcatcctt gactacgaga agaaatataa aatagaaaaa
     8641 cgcacttata gcaatggcta agatgagcct tgcaataagc attactcctg acaattgact
     8701 tatgcaatag gcaacaagcc cacagagcgc tcctcccgca agataatgga ataatctttt
     8761 tcgagcaaaa atcgtaagaa tcgcctttct cccacagaag tatgcatgcg cgacaaggac
     8821 cgatgcctct cccgccaaca tgcccatggc tgcgccggcc tggccaaagg gctttaccgt
     8881 aaagcacagc acaaggccgg tcactactat cccgatgctg aaagaaacca gcatagactt
     8941 ttctttctgc tgtggtatca atatttgcat ctgaagcgca ttcgagaatg ccactatcgg
     9001 tatgatccac gccgttattc gcatcgaagt tactgcaggt gtaaattgag ctccggcaaa
     9061 tacttttatc aaatcatcgg caat
//
LOCUS       DAOOWY010000073         9029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_7672_length_9029_cov_25.855248, whole genome shotgun sequence.
ACCESSION   DAOOWY010000073 DAOOWY010000000
VERSION     DAOOWY010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7672_length_9029_cov_25.855248"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK105_06350"
     CDS             <1..230
                     /locus_tag="PK105_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61368.1"
                     /translation="KRASPPTRVSAMASIDMMLFYTLCDLLWSTGTAHVFGLLGNGSS
                     KNYDLAHNNFQQSQSMPIVFYEIVSKIATLL"
     gene            325..3708
                     /locus_tag="PK105_06355"
     CDS             325..3708
                     /locus_tag="PK105_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU61369.1"
                     /translation="MLRLSLENDAPQFVRKCAVNVAAVLGFRSVSMEFPILLPDARAS
                     ARKDDLVFLVCAAAKASFAWSITRNGALVELEMRDGEAACDLLHALLEHFSEIEDSMG
                     FKLDSSQAARGCVGSPPSPVCKPGLMESGYHLVAAERGLESLFEREFLVKDKDFDFLP
                     DHIDATIELPDDFDYDELSAACDLAARLGMESLGLELPILGLAGEKHRTGSILRIERA
                     ENAALPAVLLTKENDKRVLSFRGRGNALAAMASALCSTMPGTGDDSLLAEIRDGLVSV
                     CAESGGEGDKVDLVSQEKFVELRGARIDLPWELDECRSLLAREVYPRITSGPGTEIIV
                     VIGEDIEARRVLEAEIRNACESHGAESARIRVIGAFKQGFSWLRDYELPELVACGGVA
                     SIEIGFSAFLPEGRPAWSDEDGTTPRIAPNRPDDPSAWMDAPIRLLQELYPIDDILAD
                     GLGINRDSITFSLRRETTKVPGYTLVARDARDAVVYESAFNPEFSERPYLEDFPGLGK
                     VHPGTGLVRLLKGGGTVWESHFKTDMESIWDAYQSRVLPAAREIAQAVKEQPFFSRLV
                     IEVEASEPDEALPCRHDRLSSLESLHEDLYFVGLDYFQVFGIKTKGIGYDSPGLILPI
                     IRKRSGAPEMHYSILAEGVATRVETVGSVSSPMPTSVFVDELTFDEASGRFSPRFCIR
                     FEDSAVGHSSALIGIERYAWLLSKAMAGLSQIGRAFSSVRFTFDGDEKGALVEALLPE
                     PKAFTGDFDIAELDLPKDRLIDPDVFEVISQRLSRTKGIRQRLVGRSREGRPIWAIEL
                     LPKLPGYVSRTKLIATRPTLYVNGRHHANEVSSTNSSLMFIQRLLSDSAYANLSEHLN
                     VVVLPLENVDGAALHSYLAHDNPEWILHAARYDSLGGEFARDYFNDATAHPEALAFTR
                     LWRDWLPDVVADDHGVPSHEWCQQFSGYTSPWFKGFWMPRALLYGYFWYVTDEAYAAN
                     KLVAERIEEVVADAMGAHDEFALLNAEWRDRFEKYAHAWMPKLFPAEYYKDVIFYWVP
                     YPYKPDYYYASVRFPWITAVSFVSEVADETATGGYLGLCARAHLEEDLAIVKLLSEAV
                     VHMDESVWIEDNVLCARRVRKRPLTYSKPQAPGGNPAPK"
     gene            3805..5421
                     /locus_tag="PK105_06360"
     CDS             3805..5421
                     /locus_tag="PK105_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOU61370.1"
                     /translation="MNRNERLQRRYRIVGILGSLVFHGAILWLPLYSTFTYVENAASP
                     SLRIGLIELSIPEAMREISQLPAKQGKTKANANRPSSSQEGVSQISSKEEVSQISSQQ
                     EVSQIQDVFPMEELQALAQQPVPPLLSNCEKDESPLFFEKIADLPETRQSEVIQTSAI
                     QARAPDPEPSELSFVVLQPEEAKEKEPVVKAAASVEAPVEAPLVSAGPSENTRTTEKA
                     HVVAPAIPSRQYFVPSKSELLAQKRNSLGGFSAQTVEFADLSVAESDLGSAQAASAEG
                     YLSSAAQVAELKAERITLYNKMRYYDMADSGTQPSQQSQPSQQSQLFQPDSPAPPFQK
                     AQQFALELASQNIVADSEIEMMTVPKKEKPEKREPMQTRPQSDSDSGLVPPAALSEKK
                     GLASASEVPTAEEIFRSAVPDKSPPIQGPYIKEADMASSIVEASSYYSERPHFDTTET
                     LSQRIANELSTNKEYPPAALKRKTEGIVKLSLDVAPNGSLIRVKIQTPSGSAVLDEAA
                     LKLVQSIFPLDIKLASAVSLIVPVEYRIRK"
     gene            complement(5428..6537)
                     /locus_tag="PK105_06365"
     CDS             complement(5428..6537)
                     /locus_tag="PK105_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61371.1"
                     /translation="MKKLLSKVQRKMLAVGFMLVLAALTDCSLLSLQNGTSASLFAVD
                     SKNGNVYEIDTEKAQSASIPLVSIKQNATGEMIVNGTKAFIAVGSYNNTSPGLYWFDL
                     SSSSPSVQKIGGNISAQYLCIASNNKGYVSTADYSGVYSNTVYGFNPSNPSAGLGSEV
                     TGFDSGFYPQDLVFADDGNENGYVFVTDNANSKVYRLNAAGTAVDLSFVTSSGGTTGL
                     LPGSYDWDDNGSNDAGVFVANTGGYDTNWNALPGSIDFIPLDASSGNDIVSVQKDLSV
                     ARLTAFDKNHLVGTNYSQTWIIDLTKPENDSGRLAEVKSNGASFGSLDTNAYGGYVYV
                     PDGMQTVYRFDSDGMVTAISVGTSGEMITNVAVRE"
     gene            complement(6561..8546)
                     /locus_tag="PK105_06370"
     CDS             complement(6561..8546)
                     /locus_tag="PK105_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU61372.1"
                     /translation="MYVLRSPVILVAICLFNLLLPTAFPQTVELPSVIVEGVLPMSDL
                     APSAGTTSIDSATIEATAASNLPALLSSVSGVISSPTGANGAQTTISLRGSTSNQVLV
                     LVDGVRITDPATGQTDLSRLDIPLDQIERVEIQRGALSAQYGADAVGGVIHIHTKRKT
                     TTPSVELSIRNNAFVPASVTVGSGLSATTVPFVGLALVDGQSFDISARMNNLSAWGKA
                     QRASNAYPYYDTNGERRQRANAGLLAAAAGFQGSFPLSVGELSTTARVNYRSLGVPGT
                     EDMPTPKAHQEDWNANLSAHYGTDALFAGALALDVNSYAQAGGIEYRESDTSSADIHY
                     SYRTGLDSSFSWLTPWQGELKGGTSFRYDRLESSIVKTNEGSSPQRFTGGAFIEPHIE
                     ICRWTAIPAIRYDLTSDSPSGISASLGLLYDMAKDTVFRSSISSAYRAPSFDDLYWPA
                     SGGAQGNPDLEPETAYSCDFSIAHRDERKSWSLGAFARYVQDVILWQPDPSGTWKPTN
                     YGNALYPGIETEASSNIGTWRISLNYTFLYSFVLSGNLTLADDKRIPYVPVHSASLSA
                     SRSSENFKSSFIFSYKSLRYTTTGNRAYLPAALIADARLEWIFGTNTKLGLSVQNLFD
                     ERYQVVQGYPMPGFSVSAILTMCFQGQKGLRNENSEF"
     regulatory      complement(8701..8893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         2171 a         2225 c         2378 g         2255 t
ORIGIN      
        1 tgaaaagagc gagccctcca acgagagtca gcgccatggc cagtatcgac atgatgctat
       61 tttatacctt atgtgattta ttgtggtcta ctggaacggc tcatgttttt ggcttattgg
      121 ggaatgggtc ttcgaaaaat tatgatttag ctcataataa ttttcagcaa agccaatcaa
      181 tgcctattgt gttttatgaa atagtcagca aaatagcgac cttactttga cagatgagat
      241 gttgaaggcc taaatctact accgtttcat cgaggcagtg tctgagccat ttatgactgt
      301 ggttaagcat gatattctat ttatatgcta aggcttagcc tagagaatga tgcgccacaa
      361 ttcgtgcgaa aatgcgccgt caatgtggcc gcggtgttgg gcttccgatc ggtttccatg
      421 gagtttccca tcctcctgcc tgacgcgaga gcctccgcaa ggaaggatga tctggttttt
      481 cttgtctgtg cggcggctaa agcctcattt gcatggtcta tcacacgtaa cggagcgctc
      541 gtcgaactgg aaatgcgaga tggtgaagca gcttgtgacc ttctccatgc ccttcttgag
      601 catttttccg agatcgagga ctccatgggc ttcaaacttg attcgagcca agctgcgaga
      661 ggttgcgtgg gatcgccccc gtcgcccgtt tgcaagcctg gacttatgga gagcggttat
      721 cacctcgttg cagcagagcg cggccttgag tccctctttg agcgcgagtt tctggtgaag
      781 gataaggatt ttgatttcct tcctgatcat atcgatgcca cgatagagct gcccgacgac
      841 tttgactatg acgagctctc tgccgcctgc gacctcgccg ctcgccttgg aatggaaagc
      901 ttgggacttg agttgcctat tcttggattg gctggagaaa agcatcgaac tggcagtatc
      961 ctgcgcatag aacgggctga gaatgccgct ctgcctgccg tgttgttgac taaagagaat
     1021 gacaagcggg tcctgtcctt ccgcggccga gggaatgcgc tggctgcaat ggcttccgcg
     1081 ctttgcagca caatgcctgg aacgggcgat gactctcttc tagcggaaat acgagatggt
     1141 ttggtttctg tatgtgcgga aagcggaggc gaaggagaca aagtcgatct agtctcgcaa
     1201 gagaagttcg tggagctccg aggagctcgg attgaccttc cctgggagct cgacgaatgt
     1261 cgctccctac ttgcccgcga agtttatccg cgcattacct ctggtccggg tacagagatt
     1321 atcgttgtca taggcgaaga catcgaagca aggcgcgttc tcgaggcaga gattcgcaac
     1381 gcttgcgaaa gccacggcgc agagagtgcg aggatccgtg taatcggcgc cttcaagcaa
     1441 ggtttctcat ggttgaggga ctatgagctc cccgagcttg ttgcatgtgg cggcgtcgcc
     1501 tcaattgaaa taggcttttc tgcattcctg cccgagggca gaccggcgtg gagcgatgaa
     1561 gatggcacga cgccccgcat agcaccgaac cgcccagacg atccatcggc ctggatggac
     1621 gcgccaataa ggctccttca ggaactttat cccattgatg atattcttgc cgatgggctc
     1681 ggaataaata gggattctat aacgtttagt ttgcgcagag agaccactaa agttccaggt
     1741 tatacccttg tcgcgcgcga tgccagggac gccgtggtct atgaatctgc ctttaatccg
     1801 gaattctcgg aaagaccata tcttgaagat ttccctggtc ttgggaaggt ccatccaggt
     1861 acaggtcttg ttcgcctctt gaagggggga ggtactgtct gggagtcgca tttcaagact
     1921 gacatggaga gtatctggga cgcgtatcag tccagggttt tgccagccgc gcgcgagata
     1981 gctcaagctg tgaaggagca gccctttttc tctcgcctcg taatcgaggt cgaagcaagc
     2041 gagcccgacg aggcgcttcc ttgccgtcat gaccgcctat cttcccttga atcgcttcat
     2101 gaagacctct attttgtcgg cctcgactat tttcaggtat ttggaatcaa aacaaaagga
     2161 attggctatg attcgccagg tctcatcctg ccaattatta ggaagcgatc aggtgctcca
     2221 gagatgcatt attcgattct agccgaagga gttgccacgc gagtagagac tgtgggaagt
     2281 gtctctagtc ccatgccaac ttcagtcttt gtggatgagc ttacttttga cgaagctagt
     2341 gggcgtttct cgcctcgctt ttgtatccgg ttcgaagact cagcggtggg acattcatct
     2401 gcattgatag gaattgagag atatgcttgg ctcctgtcaa aagctatggc tggtctttct
     2461 caaattggaa gagcatttag ctcagttcga ttcacttttg atggagatga gaagggagct
     2521 ttggtggagg cgctgctccc agaaccgaaa gcttttaccg gtgatttcga tattgcggaa
     2581 ctcgatcttc cgaaggacag gctcattgat cctgatgtat tcgaagtgat ctctcagcgc
     2641 ctctcaagga caaaagggat tagacagaga cttgttggaa ggagtcgaga aggcaggcct
     2701 atttgggcca tagaactttt gccaaaattg cctggatatg tttcacgcac caagctcatt
     2761 gctacgaggc caactcttta tgtaaatggc cgccatcacg ccaacgaggt ctcgtctaca
     2821 aactcgtcac taatgttcat acaaaggctg ctctcggatt ccgcctatgc aaatctttca
     2881 gaacatctca atgtcgttgt ccttcctttg gagaatgtag atggtgccgc tctccattca
     2941 taccttgcgc atgacaaccc ggaatggata ctacatgcgg cgcggtatga ctcgcttggt
     3001 ggcgagtttg ctcgcgacta tttcaacgat gcgaccgccc accccgaggc tcttgctttt
     3061 acgcggctat ggcgtgattg gctcccagat gttgtggctg acgaccatgg tgtgccgagt
     3121 cacgagtggt gccagcaatt ttctggctac acatcgccct ggttcaaggg cttttggatg
     3181 ccgcgagcct tgttgtatgg ttacttctgg tatgtaactg acgaggctta tgcggcaaac
     3241 aagctcgtag ccgaacgtat cgaagaggtc gtcgccgacg cgatgggagc tcacgacgaa
     3301 tttgccttgc taaatgcgga gtggagagat cgattcgaga agtatgctca tgcatggatg
     3361 cccaagctct tccctgccga atactacaag gatgttatct tctattgggt gccttacccc
     3421 tataaaccag actattatta tgcatccgtg cgatttccat ggattactgc ggtgagcttc
     3481 gtctccgagg tcgcagacga gacagccacc ggaggctatc ttgggctttg cgctcgcgcc
     3541 caccttgagg aagacttggc tatagtcaaa cttctgtccg aagccgtggt tcacatggac
     3601 gagtcagtct ggatcgaaga caatgtcctc tgtgcgcgcc gtgtgaggaa gaggcccctc
     3661 acctattcta aaccacaagc gcctgggggg aatccggcgc caaaatagct cagcaactat
     3721 ttccttttat tggactagat aaagcgatga agcacgacgg ttcggcgcaa aaaaccaaga
     3781 gaaatgacta ggccggagaa aaatgtgaat cggaatgaga gactgcagag acgctaccgg
     3841 attgtaggga ttcttggctc tctcgttttc catggagcaa tcctttggtt gccactttat
     3901 tctactttta cttatgtaga gaatgccgct tccccatcgc taagaattgg gttgatcgag
     3961 ctatctatac ctgaagcgat gagggagatt tcgcagttgc cggcaaagca gggaaaaacg
     4021 aaagccaacg cgaatcgtcc ctcatcgagt caggaaggag tttcccagat atcgagtaag
     4081 gaagaagttt ctcagatatc gagtcagcaa gaagtttccc agatacaaga tgtttttcca
     4141 atggaggagt tgcaagcgct ggcgcagcag cctgtacctc ctcttctaag caactgcgaa
     4201 aaggatgaaa gccccttatt tttcgaaaaa atcgccgatt tacccgaaac caggcaatct
     4261 gaagtcattc agacaagcgc gatacaagct agagctcccg atccggagcc atctgagctt
     4321 tcttttgtcg ttttgcaacc agaagaagcg aaggagaaag agcctgtcgt caaggcagcg
     4381 gcatcggttg aagctccagt tgaagctcct ttagtttctg ctggaccttc ggaaaacaca
     4441 aggacaacag agaaagcaca tgttgttgca cctgcaattc cgtctaggca atactttgtt
     4501 ccctcgaaaa gtgaactatt ggcgcagaag cgtaattcgc ttgggggctt ttcagcgcaa
     4561 actgtcgagt tcgcagatct gtcggtagcc gaatccgatt taggcagtgc gcaggcagct
     4621 tcagctgaag gctacctttc gtcggccgct caggttgcgg agcttaaggc agaaagaatt
     4681 actttataca ataaaatgag atattatgat atggccgatt caggcactca accatctcag
     4741 caatctcagc catctcagca atctcagcta tttcagccag attcgccagc tccccctttt
     4801 cagaaagctc aacagtttgc actggagcta gcgtcgcaga acattgtggc cgactctgaa
     4861 atcgagatga tgacagttcc taagaaagaa aagccagaaa agcgagaacc catgcagaca
     4921 aggccgcaat ctgattcgga ttcagggcta gtgcctccgg cagccctatc ggaaaagaag
     4981 ggattagcaa gcgcttccga agtcccgaca gccgaggaga tattccgtag tgctgtacca
     5041 gataagagtc cgcccatcca gggcccatac ataaaagaag cagatatggc atcttctatt
     5101 gtcgaggcta gctcgtatta ttcagagaga ccacattttg acactacaga gacactttcg
     5161 cagcgaattg ccaatgagtt gtcgaccaat aaggaatatc ctccggcagc ccttaagcga
     5221 aaaaccgaag gtatagttaa gctttcgctc gatgtagccc caaatggcag cctcattaga
     5281 gtaaagattc agactccttc cggaagcgca gtcctggacg aggcggccct caagcttgtt
     5341 cagagcattt tccctttgga catcaagttg gcctctgcgg tatcgctgat agttccagtc
     5401 gaataccgta ttcggaagta gctttcgtca ttcccttaca gctacattgg tgatcatttc
     5461 cccactggta ccgacagaga tcgcagtgac cataccatca gaatcgaatc tatatactgt
     5521 ctgcatgcca tctggcacat agacataccc accgtaggcg ttcgtatcga gcgatccaaa
     5581 agatgcgcca ttgctcttta cttcggctag acgacccgaa tcgttttctg gctttgtcaa
     5641 atcgattatc catgtctgac tgtaattcgt ccccacgaga tgattcttgt cgaaggcagt
     5701 caacctagcc accgaaagat ccttttgcac acttactata tcgtttccgc tggacgcatc
     5761 tagcggaata aagtctatgg aaccggggag agcattccag tttgtatcat agccaccagt
     5821 attggcgaca aagacgcctg catcattgct gccgttgtca tcccaatcgt agctgcccgg
     5881 aagaagacct gtcgtaccgc cggagctagt cacaaacgaa aggtcgaccg ctgtgcccgc
     5941 agcattgagc cggtatactt tgctattggc attgtctgtc acaaagacat acccattttc
     6001 gttgccgtca tctgcgaaga ccaaatcctg aggatagaat cccgagtcaa agccagtaac
     6061 ttcgctgccc aatcccgctg aaggattgct tggattaaag ccgtaaactg tattggaata
     6121 gactccgcta tagtctgctg tacttacata tcccttattg ttcgaggcga tgcaaaggta
     6181 ttgcgcactt atgttgcctc cgattttctg taccgatggg gagctgctcg aaagatcgaa
     6241 ccagtagagg ccggggcttg tgttattgta gcttcctacc gctatgaagg ccttcgtgcc
     6301 atttacgatc atctcgccgg tcgcattctg ttttatgctg acgaggggta tcgaggcact
     6361 ttgggccttt tcggtgtcga tctcatagac atttccattt ttcgagtcta ctgcgaacaa
     6421 acttgccgat gtcccgtttt ggagagacag caagctgcaa tccgtcagcg cggccaagac
     6481 aagcatgaac ccgacagcga gcatttttcg ctgtaccttt gacaagagct ttttcataca
     6541 actcctccca tatatttatc ttagaattcg ctattttcat tccgcaggcc tttttgcccc
     6601 tgaaagcaca tggtcaatat tgcagagacc gaaaagccgg gcatgggata gccctgtact
     6661 acctggtatc gctcgtcgaa gagattttgg accgacagtc ccagctttgt attggtaccg
     6721 aagatccatt cgagccttgc atcggcgatg agcgccgcgg gcagataggc tctgttgcct
     6781 gtagtggtgt atctgaggct tttataggag aaaataaagg aactcttgaa attttcgctc
     6841 gatctgctcg cgctgagaga tgccgagtgg actggcacgt agggtatgcg cttgtcgtct
     6901 gcgagcgtga ggttgcccga caagacaaaa ctgtagagaa atgtgtaatt caaactgatt
     6961 cgccatgtac cgatattgga cgaagcttct gtctcgattc ccggatacag agcatttcca
     7021 taattggtgg gtttccaagt acctgaaggg tcgggttgcc aaaggattac atcctggaca
     7081 tagcgagcga atgcgcctaa agaccatgac ttccgttcgt cgcgatgtgc gatggaaaaa
     7141 tcacaggaat aggcagtttc gggttcaaga tctggattgc cctgtgcacc gcccgatgcg
     7201 ggccagtaca ggtcgtcgaa ggagggagcc ctgtaggcgc tcgagattga gcttcggaag
     7261 acggtatcct ttgccatgtc ataaagaagc cccaagcttg ccgaaattcc cgagggagaa
     7321 tcgcttgtca ggtcgtaacg gatagcggga atggcggtcc atctgcagat ctcgatatga
     7381 ggctcgataa aagctccgcc tgtaaaccgt tgaggagagc ttccttcgtt tgtcttgact
     7441 atactcgact ctagacggtc gtagcggaaa gaagttcccc ccttgagctc tccctgccat
     7501 ggtgtcagcc aagagaatga gctgtctaaa ccggttcgat atgaataatg tatgtctgca
     7561 ctcgaggtat ccgattcgcg atactcgatt cctccagctt gagcatatga gtttacatct
     7621 agagccaggg caccggcgaa aagagcatcc gtgccataat gggctgaaag attggcattc
     7681 caatcctctt gatgggcttt cggagtcggc atatcttccg ttccaggcac tcccaatgag
     7741 cgatagttca ctcttgccgt tgtagatagc tctccgacag acagaggaaa ggaaccctga
     7801 aagccggcag ccgcagctag caaccctgca ttcgctctct ggcgcctctc tccgtttgtg
     7861 tcgtagtaag gataggcatt cgaggcccgc tgtgctttcc cccatgcgga gagattgttc
     7921 attcgagcgg agatatcgaa ggattgtcca tcgacgagag ctaggcctac aaaaggaacg
     7981 gttgtggccg agaggccact gccgactgtc acggaagcag gtacaaacgc attgttccgg
     8041 atggagagct cgaccgaggg agtcgtcgtt tttctttttg tatggatatg gatgactccg
     8101 ccaaccgcat ctgcgccata ttgggctgac agagctccac gctgtatttc gacgcgctcg
     8161 atctgatcga gcggtatatc gaggcgcgac agatcggtct ggcctgtcgc cgggtctgta
     8221 atgcgcacgc catcgacgag cacaagcacc tggttcgagg tggagcctct aagcgagatt
     8281 gtcgtctgtg cgccatttgc gccggtagga gagcttataa cacccgatac tgacgaaagg
     8341 agagcgggaa gattggatgc ggcagtcgct tcgatcgtag cgctgtctat gctcgttgtg
     8401 cctgcggaag gcgcaaggtc gctcatcggc aatacgcctt ccacaattac agagggcagt
     8461 tcgactgttt ggggaaatgc cgtgggcaga agaagattaa agagacagat agccaccaga
     8521 atcacggggg aacgcagaac atacataccg gatctcctcc ttgggacata agcgaattcg
     8581 agggaggaga tgagtcgtat gcctatgcaa aaatgagatg aataagaata gaacgagggc
     8641 gcccgagaag agattgctgc gcatcaagag ttcctcaatc ctcgaagttt tgcccgaaag
     8701 caggtcgggc tcgccccaga gcggtatcct ggcttccgga tcatctgtgc gcacagcgcc
     8761 ttctcacagc aatagctgca atggctgcaa agggtttttg aacctttgcc tgctgggtca
     8821 tccccggtta cagtggcggg accgctgcgg atttgcaccg cattcctcgg ttccctggag
     8881 catttcaata ataagagaca gaatcccagg cgtcaagcgc cgtggtgcta agtcggtgtt
     8941 tctcaatatt ggaatttcta aagactctat cttgctttta gcgaccgacg ggagtcgaac
     9001 ccgcttcacg agcttgggaa gctcgggta
//
LOCUS       DAOOWY010000074         8983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_7727_length_8983_cov_10.246864, whole genome shotgun sequence.
ACCESSION   DAOOWY010000074 DAOOWY010000000
VERSION     DAOOWY010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7727_length_8983_cov_10.246864"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PK105_06375"
     CDS             <1..850
                     /locus_tag="PK105_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61373.1"
                     /translation="GLSIGSVYALMAVGYSLVYSIMNFSNFAHGGVIMLGAYFGFFCL
                     TLFKLPFALAFLLASLGTLVVAIIIERIAYKPLRERKAPFLYFIISAMGTSIMLENLV
                     IATIGPTFRTYPEVFPKVPIEIGNLAIGRLDLIIFVISAVSLTLLMIFIDKTKLGKAI
                     QAASYNIKACALMGINTDIVILTVFGLGGFLAGIAGVFFGMKYTVYPQIGNITTKSFI
                     AAVFGGLGSLPGAVLGSVILGVIETFVAGYISSQFRDLIAFVILIGVLVLRPRGIMGK
                     VTEEKA"
     gene            860..1792
                     /locus_tag="PK105_06380"
     CDS             860..1792
                     /locus_tag="PK105_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61374.1"
                     /translation="MDLTYIQGILMLAGINMITVLGLSLLTGFTGLFSFGHAGFMAIG
                     AYIGTWISGSPASTPAGLGLPFIFSILGGGIAAGLVSYLIGRITLKLKGDYFCIATLG
                     FGEAIRLIFNNVNRFGGARGWPGIPGRSTFLAILIADVIAVLVLANIVNSKHGRNMIA
                     VREEELAAQTAGIDVFRYKMISLVISAIYAGVAGVFLAHYMTFIQPKMFSLTKSTELT
                     IIVIFGGLGSISGSVLGALVLTALPEILRAFEIWRLVFYGAAVILIMVSRPKGLMGGM
                     ELTPSGIRKASAERNALREAKAKLLVEAQDGEEQ"
     gene            1789..2577
                     /locus_tag="PK105_06385"
     CDS             1789..2577
                     /locus_tag="PK105_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61375.1"
                     /translation="MTSSPILHIKELTKNFEGLIAVDNVSFDVPEGGIIGLIGPNGAG
                     KTTIFNLITGIYKPTSGSIEFKGNSLVGLEPFRIAEKGITRTFQNIRLFKNLSVFDNV
                     LTACHRSANYNFAEAVFRLPRFRYQEKELKEKAEQLLDIMGLQCYKDLIANNLPYGLQ
                     RRLEIVRALALDPTLLLLDEPAAGMNPDETEQLMSLIGKIRDDFKLSVLVIEHHMDLV
                     MGVCENIVVLNFGEKIAEGNADEISKNPLVIEAYLGTPEEAANA"
     gene            2570..3292
                     /locus_tag="PK105_06390"
     CDS             2570..3292
                     /locus_tag="PK105_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61376.1"
                     /translation="MLEIKNLHVAYGGIRALKGVSLSVQEGEIVTIIGANGAGKSTLL
                     NTISGFLRPAKGTILYKGKTLPRRPDLIVKAGICQVPEGRLVFANLSVLDNLMLGAYR
                     RNDKKAVRNDLKKVFDIFPRLEERKSQRAGTLSGGEQQMLAMGRALMTNGELILMDEP
                     SLGLAPLLVQTVFEIIEQFRGLGKTILLVEQNAFKALQVADRAYVLEQGRVVKQGLSR
                     NLAQDAAVKEAYLGSRKRNGGG"
     gene            complement(3295..3909)
                     /locus_tag="PK105_06395"
     CDS             complement(3295..3909)
                     /locus_tag="PK105_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU61377.1"
                     /translation="MPDFRTNEPIAIILAAGCSSRMGATKPLVEIEGVPLLLHAARCF
                     QEAGIEEIIVVTGFDSDNVGKLASLHGIRPIFNKDYQKGMFSSICAGLRAVSSNCDSA
                     FILPVDIPFVLPSTVVKLKNAIKDKPIVIPRYEKVSGHPPIIHSALFSLVERWNGKNG
                     LRGFFGHRQADIEYLEVDDYNILSDIDTEADLRELLLKRAELRS"
     gene            complement(3949..5076)
                     /locus_tag="PK105_06400"
     CDS             complement(3949..5076)
                     /locus_tag="PK105_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOU61378.1"
                     /translation="MRELRSWIAERLNNDQTVVISAITHASGSTARGTDALMAVDMNG
                     KMEGTVGGGYIENQAIIAIRNMLEAGYDHRDLFFDLSPEAQQNQITCGGKVSLHLERI
                     DQQSKARSALKSCLEQVESGNPCAFFVARTPGEKYVSAIDERGNVLYPHQQNSCEELI
                     AKSIIESSKKIQTCLDGARTLSGASCFELELPEKGISNIFQVFLLGFRPDPVVHIFGA
                     GHVGKATSEVASLVGFRVIVSDDRPELLTRERFPKANMLRIIDDFADPFTASKGAPAI
                     EIGPQDCAMILTRKPDIDEKMLSCLLRTRAGYIGLIGSKTKRDGIFAALRDEGFSETD
                     LSRVHSPIGLEIGAQTPEEIAISIIAEVIADRSGVLPKLKY"
     gene            complement(5081..7474)
                     /locus_tag="PK105_06405"
     CDS             complement(5081..7474)
                     /locus_tag="PK105_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU61379.1"
                     /translation="MIDNNDPIKPMDSETLKATQKFNVVAHDIAKIDGEGLVLGRPAF
                     ADDLAPANALYVKLVRSPHAFARITSIDPSEALALPGVACVLTWKDCPRVPITRAGQG
                     NPEPSPHDRFILDEYVRYVGDEVAVVAAEDEVTAEKAAMLVKVQYEVLQPILDFEKAL
                     DNPIIIHPEPEIHEMFPIGFEPKRNIAAAYNMEIGNVEQELANSPVYVETTVHTQAQQ
                     HVALESHTAFSYIDIHGRLVIVTSTQNPWHTRRLLALALQMPIRQIRIIKPRIGGGFG
                     GKQHIHIEPYVAMVTMKTGRSARLALTRREVFEATFTRHEMRVKVRLGANSDGKLNAI
                     DMQVLSNTGAYGEHALTVFMVAGSKTLPLYNKVNAVRFGGHVVYTNKVSAGAFRGYGA
                     IQGLTGLESAMDELAHKIGIDPLELRRKNMIHEGETSDVFRIMGEGTEGVAMTIESCK
                     LEECIARGKELIGWDPNRLVWDVAPGKVRAMGMAIAMQGSGLPLIDMGSARIEFQDGG
                     FFKLHIGATDLGTGSDTILAQIAAEELGVDVENIIVYSSDTDNTPFDVGAYASSTTYV
                     SGSAVIKAARSLKKKLIEAVAQKFEVDPAFVLFDNKTFKTLNGGKSLSLYDFSFDTLY
                     HDGERMKTLEATESFTGAKSPPPYLAGFVEIELDKETGKVEVVHYVSVADVGTPINPR
                     LATIQIEGAALQGIGLALYEDVRYSERGHMLSHNLLTYAIPSREDVGKITVELVDSYE
                     PSGPFGAKSAGEIGIDAPPAAIGNAIRNAIGVRLTEYPFTPERVLMAIDRAKAEGAN"
     gene            complement(7467..7922)
                     /locus_tag="PK105_06410"
     CDS             complement(7467..7922)
                     /locus_tag="PK105_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOU61380.1"
                     /translation="MTITLYINGNEKILDCQPGDMLTEVLRKAGYVEVKKGCDTANCG
                     ACTVLIEDKPILSCSYLAVRADGKRITTIQGVAEEAKEFARFLTAEGADQCGFCAPGF
                     ALTVLSMKRELRNPSEAEIRHYLSGNLCRCSGYEGQIRAIKKYLEAVHD"
     gene            complement(7919..8707)
                     /locus_tag="PK105_06415"
     CDS             complement(7919..8707)
                     /locus_tag="PK105_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU61381.1"
                     /translation="MEIREYARPKSMDQAMHLICEEHGTAIGGSAWLRTSTKTVMLGV
                     DLCDLGLEYIREFQDHIEIGAMTTYRELETSPLLDSRFGKIFRTTVSHIVGVQLRNII
                     TVGGTVAGRYGFSDLNTTLCAIGARVVFYPNVEKDLSSFIEKGYASPFLIEKIILPSS
                     ARAAYQQMRISESDFPIINTAVAWTGTVWRIAVGSRPAATRICRNAMKILGDSANPSD
                     EQIEAAAAEASKETQFGSDIRASANYRQKILPVLVKRALLEVRG"
BASE COUNT         2237 a         2075 c         2150 g         2521 t
ORIGIN      
        1 tggcttatcc ataggctctg tctacgctct catggcggtt ggatattcgc ttgtctattc
       61 gataatgaat ttttcgaact ttgctcatgg tggagtaata atgctaggag cctattttgg
      121 attcttttgt cttacgctat tcaagcttcc ttttgcgctg gcattcttgc tcgcttcgtt
      181 gggaaccctt gtagttgcca taattataga acggatagcc tataagccgc tgcgtgagcg
      241 aaaagctccc tttctttact ttattatttc ggctatgggc acatccatca tgcttgaaaa
      301 ccttgtaatt gcgacgattg ggccaacttt tcgcacctat cccgaagtat tccctaaggt
      361 tccaatcgaa attggtaatc tagccattgg aaggctggat ctcataatct ttgtgatttc
      421 cgctgtgagc ctcactctcc taatgatttt tatagacaag acaaagctgg gcaaggcaat
      481 acaagccgcc tcatacaata tcaaggcctg cgccctaatg ggaataaata cagatattgt
      541 tatccttacc gtgtttggcc ttggggggtt tcttgcaggc atagcgggtg tattttttgg
      601 catgaaatat acggtttatc cacagattgg caatattact accaaatcgt tcatcgcggc
      661 tgtgtttggc ggtctaggaa gcctgccggg cgcggtgctt ggctctgtaa ttcttggtgt
      721 gattgagact tttgttgccg gatatatctc atcgcaattt cgagacctaa tagccttcgt
      781 tattctgata ggcgttctgg ttctgagacc gaggggcata atgggtaagg ttactgagga
      841 aaaggcttag ggggtgagca tggatttgac atatattcag ggaattctga tgctcgcagg
      901 cataaatatg attacggttc taggcctaag tcttttgacg ggttttacag ggctattctc
      961 gttcgggcat gctggattca tggccattgg agcctatatt ggaacttgga tcagtggctc
     1021 tccagcatct acgccagcgg gccttgggtt gccttttatc ttttccatat tgggtggggg
     1081 aattgccgcg gggctggtga gctatctgat aggcagaatt acccttaagc tcaaaggcga
     1141 ttatttctgc attgcgacgc ttgggtttgg cgaagcaatc cgccttattt tcaataatgt
     1201 gaatcgtttt ggaggagcca gggggtggcc cgggattcct ggcaggagca cttttcttgc
     1261 catccttatc gcggacgtca ttgcggtgct tgtcctcgca aacattgtaa attcgaagca
     1321 tggtcggaat atgattgctg tgcgagaaga ggaattggcc gcgcaaaccg cgggcataga
     1381 tgtatttcgc tacaagatga tctcacttgt aataagtgca atatatgctg gagtagcggg
     1441 agtttttttg gctcattata tgacgttcat tcaacccaag atgttctctc tcaccaaatc
     1501 taccgaactc acaatcattg tcatattcgg aggacttggc tccatttcag gctcggtctt
     1561 aggagctttg gtgctaacgg cacttcctga gattctgagg gctttcgaga tatggcggct
     1621 cgtattctat ggagcagcag tcattcttat tatggtgagc aggccaaagg gtctaatggg
     1681 tggtatggag cttacgccat cagggattcg caaggcgagt gcagaaagaa acgccctgcg
     1741 cgaggcaaaa gcaaagctcc ttgttgaagc ccaagatgga gaagagcaat gacaagttct
     1801 ccaattctcc atatcaaaga actaacgaag aactttgagg ggctaatcgc agtggataac
     1861 gttagcttcg atgttcccga aggaggcatt atagggctaa taggtcccaa tggcgctgga
     1921 aagacgacta ttttcaactt aataacggga atatataagc caacatctgg ctcaatcgag
     1981 tttaagggca attccttagt gggccttgag ccattccgaa ttgcagaaaa aggcataact
     2041 cggacatttc agaatataag gcttttcaag aacttaagtg tattcgacaa tgtactgaca
     2101 gcctgtcata gaagtgcgaa ttacaatttc gcggaagcag ttttccgcct tcctcgtttc
     2161 cgatatcagg aaaaggagct aaaggaaaaa gcagagcagc ttttagatat tatggggctc
     2221 caatgctata aggaccttat tgcaaacaat ttgccttatg ggctgcaacg aaggttggag
     2281 atcgtaagag cgcttgcgct tgacccgacg cttttattat tggatgaacc agctgcaggt
     2341 atgaaccccg atgaaaccga gcagcttatg agtcttatcg gcaaaatacg agacgatttc
     2401 aaactctcgg ttcttgtaat agagcatcac atggacctgg ttatgggtgt atgtgagaat
     2461 attgttgttc tgaatttcgg cgagaaaatc gccgaaggca atgcggacga aatatcgaag
     2521 aatcctctgg tcatcgaagc ctatctcgga accccggagg aggcagccaa tgcttgagat
     2581 aaagaatctg cacgttgcct atggtggtat tcgagcgcta aaaggcgttt ctctttctgt
     2641 gcaggaaggt gagattgtca caattattgg agctaatggc gcggggaagt cgaccttgct
     2701 gaatacgata tccggatttt tgcgtccggc aaaggggaca attctgtata agggtaagac
     2761 tctccctcgg cgcccagact tgattgtcaa ggccggtatc tgtcaagtgc ctgaagggcg
     2821 cttggtattt gctaatctga gtgtactcga taatctcatg cttggagcct atcgacggaa
     2881 cgacaagaag gctgtccgca acgatctcaa aaaggtcttt gacatttttc ccaggctgga
     2941 agagcgtaaa tctcagagag cagggacact ctctggagga gaacagcaga tgcttgccat
     3001 ggggcgtgcc ctcatgacaa atggagagtt gattcttatg gatgagccat ccttaggcct
     3061 ggctcctctt ctggtccaga ctgtattcga aataatcgag caattccgag ggcttggcaa
     3121 aactatactt cttgtggaac agaatgcatt caaagcactt caagtcgcag accgagctta
     3181 tgtgcttgaa cagggaagag ttgtaaaaca agggctatcc cgcaacctgg ctcaggatgc
     3241 tgcagttaaa gaagcttacc ttggttcgcg taaacgaaat ggaggaggct aaactcaaga
     3301 gcgcaattcg gctcgcttca ggagtagctc tcttaggtca gcttctgtat cgatatcgct
     3361 aagtatatta tagtcatcaa cctccaggta ttcgatatcg gcctgtctat ggccaaagaa
     3421 tcctcttagc ccattctttc cattccacct ttctaccagg gagaacaaag cactatgaat
     3481 tattggcgga tggcctgaga ccttttcata tcgaggaatc actattggtt tatccttaat
     3541 ggcgtttttg agcttgacca ccgtgctcgg caatacgaag ggaatatcta ccggcagaat
     3601 aaaggcggaa tcacaattgg aagaaacggc gcgcaaacca gcgcaaatag aagagaacat
     3661 tcctttttgg taatccttat tgaatatagg ccgtattccg tgcaaactag cgagtttccc
     3721 cacattatcc gagtcaaaac ctgtcactac aattatttcc tcaatccctg cttcctgaaa
     3781 gcaacgagcg gcatgcaaaa gcaagggaac tccctcgatt tccacaagag gtttggttgc
     3841 tcccatacga gaagagcatc ccgctgccag aatgatagca ataggttcgt ttgtcctaaa
     3901 gtcaggcata cagataaaat cctctagtac cgagcgaata cccaagggtt agtatttgag
     3961 cttgggcagc acacctgatc gatccgcaat tacttccgct atgatgctta ttgcaatttc
     4021 ttccggcgtc tgagctccta tttcaagtcc gattggagaa tggactctcg aaagatcagt
     4081 ctctgagaag ccttcatctc gaagcgcagc aaatatgcca tctcgcttgg ttttggaacc
     4141 aataaggcca atgtagcctg ctcttgttcg caaaagacat gataacatct tttcatcgat
     4201 atcgggtttt cgagtgagta tcattgcgca atcctgagga ccaatttcga tagccggcgc
     4261 acctttagat gctgtaaagg ggtctgcaaa atcatctatt atcctgagca tattagcttt
     4321 tggaaatcgt tctcttgtga ggagttccgg tctatcatcg cttacaatta ctcggaaacc
     4381 aaccaggctt gcaacctcgc tcgttgcctt gccaacatgg ccggcgccga agatatggac
     4441 gaccggatct ggtctaaacc caagtaggaa gacctgaaag atattagata tgcctttttc
     4501 aggcaattct aattcgaaac aagaggctcc ggacagagta cgggcgccgt ccaagcaagt
     4561 ctgtattttt ttactgctct caatgatact cttggctatc agctcttcgc atgagttttg
     4621 ttgatgcgga tataagacat ttcctctctc atctattgca gagacatatt tttctccagg
     4681 agtcctcgca acaaaaaaag cgcatggatt gccagattcc acttgctcaa ggcatgattt
     4741 aagggcactc cttgccttac tctgttgatc gattctttcg agatgcagag aaactttgcc
     4801 tccgcaggtg atctgatttt gttgcgcctc aggcgagaga tcaaaaaaga gatctctgtg
     4861 atcatagcca gcttctagca tgtttcgtat cgcaataatc gcttggtttt ctatatatcc
     4921 tccgccgacc gtaccctcca ttttgccatt catatccact gccataagag catcagtacc
     4981 cctcgccgtc gagccgcttg cgtgagtaat cgcggaaata acgactgtct gatcgttatt
     5041 caatcgttcc gcaatccagg atcttagttc gcgcattgtt ttaattggca ccttcagctt
     5101 ttgctctatc gattgccatc agcacgcgct caggggtaaa ggggtattct gtcaagcgaa
     5161 caccgattgc atttcgtata gcattgccga tggcagcagg aggagcatct attcctattt
     5221 ctccagcgga ttttgcgccg aaaggccccg atggctcgta gctgtcgacc agttcgactg
     5281 taatttttcc tacatcttcc ctggaaggaa ttgcataagt caaaaggtta tggctaagca
     5341 tgtggcctct ctccgaatag cgcacatctt cataaagggc aagaccgatg ccctgaagtg
     5401 ccgcaccttc tatttggatg gtagcaagcc tgggattgat tggagttccg acatcggcta
     5461 cggatacgta gtgtacgacc tcgactttgc ctgtttcctt gtccagctct atttcgacaa
     5521 aacctgccag gtagggagga ggggattttg cacctgtaaa ggattcggtt gcctcgaggg
     5581 tcttcattct ttctccatcg tgatagaggg tatcgaagct aaaatcgtag agcgagagcg
     5641 atttgccacc attgagagtc ttaaatgtct tgttatcgaa taggacgaac gcaggatcta
     5701 cttcgaattt ctgagcaacc gcttctatga gttttttctt gaggctacga gcagctttga
     5761 ttacggctga acctgagaca taggttgtac tagatgcata agcacctaca tcaaagggtg
     5821 tattatcggt gtcggaagag taaacgatta tgttttctac gtcgactcca agttcttcag
     5881 ctgcgatctg agcaagaatc gtatcagatc cggtaccaag atcggtagcg cctatgtgga
     5941 gcttaaagaa tcctccatcc tggaattcga ttctcgctga gcccatatcg atgagtggca
     6001 agcctgaacc ctgcatggct attgccatgc ccatcgctcg cacttttcct ggagcaacat
     6061 cccagaccaa acgattcgga tcccagccta tgagttcctt tccgcgagca atgcattcct
     6121 cgagcttgca ggattcgatc gtcatggcga ctccttcggt tccttccccc atgatgcgaa
     6181 atacatcgct ggtttcacct tcgtgaatca tgtttttgcg gcgaagttcc agagggtcga
     6241 tgcctatctt atgtgcgagc tcatccatgg cggactcgag accggtgagc ccttgaattg
     6301 ctccatagcc cctaaatgcg cctgcggaca ccttgtttgt atatacgaca tggccaccaa
     6361 agcgaacggc atttactttg ttgtaaagcg gcaaggtctt tgagccggca accatgaaga
     6421 ctgtcagcgc atgctcgcca taggcaccgg tattagagag cacttgcatg tcgatagcat
     6481 tgagctttcc atcggaattg gcgccgagcc gcactttgac acgcatctcg tggcgggtga
     6541 aggttgcttc aaatacttcg cggcgcgtga gggcaaggcg agcggatcta ccggtcttca
     6601 tagtgaccat tgcgacataa ggttcgatat gaatgtgctg ttttccgcca aatcctccgc
     6661 ctatcctggg ttttataata cgaatctgtc ttattggcat ctggagggct agtgcgagca
     6721 acctgcgcgt atgccagggg ttttgagtag aggtgacgat tacgagcctg ccatggatat
     6781 cgatgtaact aaaggccgta tgagattcaa gtgctacatg ttgttgagct tgagtatgta
     6841 ctgtggtctc gacgtagaca ggtgagttgg ccaattcctg ctctacattt cctatctcca
     6901 tgttatatgc ggcagcgata ttcctcttgg gttcgaatcc tatcggaaac atctcatgaa
     6961 tttcgggttc aggatgtatg atgatcggat tatccagagc tttttcgaaa tccaggatag
     7021 gttgtagaac ttcgtattgc actttgacta gcattgcggc tttttctgcc gtaacttcgt
     7081 cctctgccgc aaccacggca acctcatctc ccacatagcg cacatattca tcgagaatga
     7141 atcgatcatg agggctgggc tctgggttgc cctgtccagc tcttgtgata ggtacccgag
     7201 ggcaatcttt ccatgttaat acacaggcaa cgccaggcaa tgcaagcgct tccgatgggt
     7261 ctatagaagt gattctggca aaagcatgag ggcttctgac aagcttgaca tagagggcat
     7321 tggctggagc aagatcatct gcaaaggcag gtcgaccaag caccaagcct tccccatcaa
     7381 tctttgctat atcgtgagca acaacattga atttttgagt tgctttgagc gtttcactat
     7441 ccataggctt aatcggatca ttattatcaa tcatggactg cctccagata tttctttatc
     7501 gcacgaatct gtccttcgta gcctgaacag cggcatagat tacccgatag gtaatgacgg
     7561 atttctgctt cacttggatt tctgagctct ctcttcattg ataatacggt aagagcgaaa
     7621 ccaggtgcgc agaatccaca ttgatctgcc ccctccgcag ttaaaaatcg agcgaattcc
     7681 tttgcttctt cggcaacgcc ctgtattgtg gttatccgct tgccatcagc gcgcacggct
     7741 agataggagc aagatagtat aggcttgtcc tcgatcagaa cagtgcaagc gccacaattt
     7801 gccgtatcac agcctttctt tacttctaca tatcctgcct tacggagtac ttccgtcagc
     7861 atgtcgccag gctggcagtc caggatcttc tcattaccat ttatgtatag ggttatggtc
     7921 atcctcgcac ctccaaaagc gcacgtttga cgagcacagg aaggattttt tgtcgatagt
     7981 tagcgctggc tctgatatcg ctgccgaact gtgtctcttt actcgcctcc gcggcagctg
     8041 cttctatctg ttcgtcagag ggatttgcgg aatctcccaa tattttcatt gcatttctgc
     8101 aaatgcgagt agctgctggt cgagaaccga ctgcaatgcg ccatactgtt cctgtccaag
     8161 caacggcggt attgattata ggaaaatcac tctcggatat cctcatctgt tgataggcgg
     8221 ctcttgcgga agatggcaga attatctttt ctatcaagaa ggggctggca taccctttct
     8281 cgatgaagct agaaagatcc ttttctacat ttgggtaaaa gacaacacga gctccaattg
     8341 cgcatagtgt ggtgttgaga tcggagaacc catagcgccc agccactgtt cctccgacag
     8401 ttataatgtt tcgtaactga acgcctacaa tatggcttac ggtcgttcta aagatctttc
     8461 caaatctcga atcaagcaag gggcttgttt ctaattccct ataggttgtc attgcgccaa
     8521 tttcgatgtg gtcttggaat tcgcggatat attccaggcc tagatcgcat agatcgacac
     8581 cgagcatgac agtctttgta ctggttcgaa gccacgcgga acccccaatc gcagtaccat
     8641 gttcttcgca aatgagatgc atggcttgat ccatgctctt ggggcgagca tattctcgta
     8701 tttccatctg aagctccttg ataaaaagga tggttcaact atctcctaat ttttttataa
     8761 tagcataggt ttagagaatc agcaaattgg gagtatttat caacaaccta agcttggcta
     8821 tactaaaaac atggcagaat ttgtacactt acacaatcac agcgattatt cccttctcga
     8881 tggtgctgct tcaatccaga aactcataag gaaagctaag gctcttggaa tgccaggtat
     8941 tgccataacc gatcatggca acatgtttgg cgcaatgtct ttt
//
LOCUS       DAOOWY010000075         8880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_7840_length_8880_cov_13.385269, whole genome shotgun sequence.
ACCESSION   DAOOWY010000075 DAOOWY010000000
VERSION     DAOOWY010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7840_length_8880_cov_13.385269"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..356
                     /locus_tag="PK105_06420"
     CDS             75..356
                     /locus_tag="PK105_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOU61382.1"
                     /translation="MRTCITEMSKALEGADAALIEEFFYSLFTSAEADEMAKRWALVK
                     ELAKGTPQRKIAAELGLSLCKITRGSRELKKPGSAFRQLLDKLPASQAT"
     gene            362..961
                     /locus_tag="PK105_06425"
     CDS             362..961
                     /locus_tag="PK105_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61383.1"
                     /translation="MEIPLRLISRKDGSLFYGVPLFFRLIMAAMLILMIIAIGIDGFN
                     TSIVGWIIMILLLLGFVYKEDWIFDPSSRHILSQIGFYPLLKKTSIAFEEVSYLQLAA
                     ISKGTIPGSREEETANRDAFSQMRGQETGEDKKGGMRSFFQRKKLYISLLIITKNEEH
                     YLLDMIPAHRASRLSQTGKALAALIGCPFLDDLYSKSGH"
     gene            complement(979..2841)
                     /locus_tag="PK105_06430"
     CDS             complement(979..2841)
                     /locus_tag="PK105_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61384.1"
                     /translation="MNRKNSRFGFGKISMAIFSLLILKFAMGSGGSGAVYAQGHLAVE
                     LTHPVYMMIETAELRGVISRLSSVKPYTSQQVADFIAILMGHMEAFSPAEQDLIRKYA
                     EEFKIGKAIEESLWRDRSGIAQAGIRVEETTKLDVAGICDLIDGSSSTPFKDVWHFNS
                     RWVPYLKGDPLSWFSFKGEAGFTFDKIERDLYLPYEFTKEWDSNHIWFGTPRTSDGTL
                     DYPTASYDIREDIAAETDDSSVMVRLSRFRRDWGMGSGSFSLSSTARPFVGFETVLRP
                     SKYFAISGLVGSLTNWQKYDNEKSTAIEYYDKNGDGVLDIEDGDYFYYSALSYQKMLG
                     LQRMELFPFDWLTISATSTIVGAKRFELGYFSPLLFSVMYQNQLADIDNLAVQVDGQI
                     VVPRIGKFYASFYADEMEITNLSELFTKARNMFALQGGAKIPLPWWSFATLTAQYSKI
                     EPFVYAHYPTWYPDYRLRVDTSYTQDDENLGYYLPPNSDEFLVKLDMMPAPDWRLSLK
                     YSFIRHGDNPGNPGDSFIFGDVNKWLEYTTALLYMDKSFLHDGIYDYNHIGNISVSWR
                     PASPPMLFGAQIPIELSLGYGISYTWFEDGTGAGRVVPASEWKNIFSMSMKLYL"
     gene            complement(2838..3944)
                     /locus_tag="PK105_06435"
     CDS             complement(2838..3944)
                     /locus_tag="PK105_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU61385.1"
                     /translation="MPILFEPFLGNTLVARGPTNLGLYVFKQSDVARAIVIDSGGDED
                     AGRKILRECERLGVELAMIINTHSNADHCGGNAFLSKRTGCSIAATELEAAFLSYPQL
                     EPSFLSGSFPQKALRNKFLMAQPSRANCLLEPPCALTFDKDGRWQLSKPAQVHGSAAA
                     NTSQGSPINELQSASTFAPWSAPTSASAIIPKNESQSAAQGMPCLQIMSLPGHYFSMI
                     GILTPDNVFFVADSLAGAPILEKYGLFFLYDIEAELATLSMLESSEAEWFVPAHAEPT
                     QDIKSLVDINRAKIFEIADVIVSAVNSEANCAEGATLENVMAAVFSHYDIVLNANQYV
                     LVGSTIRSYLFWLCDQGKLENAFEHQRMIFRIKR"
     assembly_gap    4106..4205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4223..6406)
                     /locus_tag="PK105_06440"
     CDS             complement(4223..6406)
                     /locus_tag="PK105_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOU61386.1"
                     /translation="MDDTIDFDPTRIENLCVDEIKIAERIANELAIRLLQVQAVLDLS
                     AEGCTIPFISRYRKERTGNLDELQVRDCIQKFQSYKNLEERRLEVIKGISAMGKLDAF
                     LHENILRATTLTELEDLWSPFKKKKKTRGMLAQEKGLAPFAELMERTTILEEVVAAAT
                     SFIHRNEEHPELSVESAEDALSGARDILAERLSQDTSLRAHIKRVFIKEGELAVKGIG
                     DEEKRGQSTYQMYWDYREPLSTLKHHRVLAINRGEREGELDVSIEVSDELVEARMLER
                     VRPANAQHKQAVIDGLLRLLLPAVRREIRSDLTESAESHAIEVFSTNLRNLLMQPPLR
                     GTRVLGIDPGIRTGTKCAALDETGKFLDYFVINQETKPEQGKKDVAAAVIKYALSVIA
                     VGNGTGSHEVQKLIAESIAENGLSCRFAVVDEDGASVYSASDLAREEFPNLDLTIRGA
                     ISIGRRLQDPLSELVKIDPKSIGVGLYQHDVNQKQLASQLDDVVGSVVNQVGVNLNTA
                     SYSLLRYVSGINASLARKIVKFRDASGVIRNRYLLKEIPGLGEKTFEQCAGFLKIPES
                     DNALDNTWVHPENYAVASEILSMVKSGKEPQTKERESLKQKYKIGDITLDDIISELKK
                     PNRDPRDDLPPPILQEGVLSLEDLRPGMRVKGKVKNVVDFGAFVDIGIKENALIHVSE
                     MAEHFVKNPMEVLKVGDVKDFTVISIDAVRRRIGLSLKANLKVDS"
     gene            6562..7194
                     /locus_tag="PK105_06445"
     CDS             6562..7194
                     /locus_tag="PK105_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU61387.1"
                     /translation="MGKFSNKVRTFSALEVAKLCGVVNQTAINWIRSGYLKAFNTPGG
                     QYRVYAEDLVSFLKERDMRVPDELKDDLTMPVDSGLAIIVDDDKDLNTILKRLLERRI
                     DDLRVAQAYDGFEAGRIIAERRPAIVILDLNLPGVDGLSLCKKIRSDESFGQPAVIAI
                     TGIGEEDAQPRMMEAGADAFFSKPLNFDALIAKTKELMEARKDLEKKDGN"
     gene            7243..7806
                     /locus_tag="PK105_06450"
     CDS             7243..7806
                     /locus_tag="PK105_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOU61388.1"
                     /translation="MKELYRSDNGRCIIAVGDITNIACDAIVNAANSSLLGGGGVDGA
                     IHSAGGPAILAECRALRNSSLPNGLPPGKAVATTAGQLPARRVIHTVGPIWQGGAYGE
                     DETLASCYRECLALAVHEQLKSIAFPAISTGIYGFPKERAAKIAWNTVQDYLASTPEA
                     ASIIVWFVFFSEIDAHIFLEANGKIKL"
     gene            7803..8855
                     /locus_tag="PK105_06455"
     CDS             7803..8855
                     /locus_tag="PK105_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOU61389.1"
                     /translation="MKAALFTQFREPIEIVEVPDPIAPDDGVVLKVDATGICRSDWHG
                     WQGHDPDIRLPHVPGHELAGTIVEVGRDIKNWKLSERVTMPFVAGCGHCPPCLSSNQQ
                     VCDNQFQPGFSAWGSFAEYVAIRYADMNLVRLPESLDAATAASLGCRFATAFRALEAQ
                     SRLRAGEWIAIFGCGGVGLSAIMIATAMGARIIAIDIQEEKLKKAKEIGADIVINSRE
                     VSDIVAVIRDITHGGVQVSMDALGSPETCFSAISSLAKRGRHVQVGLMLADHKHPRIP
                     MDLVIARELEIYGSHGIQAHRYDVLLGMIEAGKLHPERLIEARLNLRQGVDFLQKMDQ
                     FPGVGISVITSMQDNS"
BASE COUNT         2326 a         2195 c         2001 g         2258 t
ORIGIN      
        1 tttgccattg tgatttttga ccctttcgag ttttcatagt ataattaata aatatgccaa
       61 gaaatcctga actgatgcgc acttgtatta ccgagatgtc caaagctctc gaaggggcag
      121 atgccgcact tatcgaagaa ttcttctatt cgctttttac ctcggccgaa gccgatgaaa
      181 tggccaagcg ctgggctctt gtaaaagaat tggcaaaagg cacaccacag cgcaaaatcg
      241 cagccgaact cggcctatct ctctgcaaga taactcgcgg ttcgagagaa ctaaagaagc
      301 ctggttctgc ttttcgtcag ctgctggaca aattgcctgc gtctcaggca acataaatag
      361 tatggaaata cctcttcgcc ttattagccg caaagacggt tctcttttct atggagtccc
      421 tctctttttt cgtctcataa tggctgcaat gctgatcctg atgataattg ccattgggat
      481 cgatggattc aacaccagca tcgtgggttg gattatcatg atccttctac tacttggctt
      541 cgtatataaa gaagattgga tcttcgaccc ttcgtcaagg catattctga gtcagattgg
      601 attctatcct ctgctaaaaa agaccagcat tgcattcgag gaagtctcat atctccagct
      661 ggctgctata tcgaagggga caattcctgg cagcagagaa gaagaaaccg ctaaccgaga
      721 cgcttttagc cagatgcgtg gccaagaaac tggagaagat aaaaaaggtg gaatgcgctc
      781 ttttttccag agaaagaaac tctatatttc gcttcttatc atcaccaaaa acgaagagca
      841 ttaccttctg gacatgattc ctgcccaccg agcctcacgt ctttcgcaaa ctggaaaagc
      901 actcgcagcg ctcataggct gtccttttct tgatgatcta tatagtaaat ctggccattg
      961 agtgcggagc actccaattc ataaatacag cttcatggac atcgaaaata tgtttttcca
     1021 ttcagatgcg ggaaccactc tgccagcgcc ggttccatcc tcgaaccagg tgtatgaaat
     1081 accatatccc aggctcaact ctataggaat ttgcgcacca aagagcatgg gcgggcttgc
     1141 tggcctccaa gaaacactga tgtttccgat gtggttgtag tcatagatgc cgtcgtggag
     1201 aaaactctta tccatataga gaagtgctgt tgtatactca agccatttgt tcacatcacc
     1261 aaaaataaat gaatcgcctg gattgcctgg attatcccca tgccgaataa aactgtattt
     1321 gagagaaagc cgccagtctg gcgctggcat catgtcgagc ttgaccaaaa attcgtcgga
     1381 gttgggcggt agataatagc caagattttc gtcatcttga gtataggaag tatcgactcg
     1441 caaacggtaa tctggatacc aggttggata atgcgcatac acaaaaggct cgatcttgct
     1501 gtattgcgca gtcagggttg caaaagacca ccacggaaga ggaatctttg caccgccctg
     1561 caaggcgaac atattccgcg ccttggtaaa gagttccgac aaattggtaa tttccatctc
     1621 gtcggcataa aaggatgcat agaatttacc tatccgcggc acaacgatct ggccatccac
     1681 ctggaccgcg agattatcga tatcggcaag ctgattctgg tacatcaccg aaaaaagcaa
     1741 gggcgaaaaa taaccgagct cgaagcgctt agccccgaca atagtgctcg tcgcagaaat
     1801 tgtgagccaa tcgaagggaa aaagttccat gcgctgaagc cccaacattt tctgatagga
     1861 aagagccgag taatagaaat agtcgccatc ttctatatcg agaaccccgt cgccgttttt
     1921 atcataatac tctatagctg tgcttttttc gttgtcatac ttctgccaat tcgtgagcga
     1981 acccacaagt ccagagattg caaaatattt cgaaggcctt agcacagtct cgaaacccac
     2041 aaaaggccgc gctgtactag aaagcgagaa agagcccgaa cccatgcccc aatcccgcct
     2101 gaatctcgaa aggcgcacca taaccgaact atcgtctgtc tccgccgcta tgtcctcgcg
     2161 gatgtcgtag ctcgctgtgg gataatcgag agtgccatct gaagtacgag gagtcccaaa
     2221 ccatatatga ttcgaatccc attcctttgt aaattcataa ggcaaataaa gatcccgctc
     2281 gatcttgtca aatgtaaatc ccgcttcccc tttgaaagaa aaccatgaca gaggatcgcc
     2341 cttgagatac ggtacccagc gggaattgaa gtgccaaaca tccttaaaag gcgtagaaga
     2401 cgagccatcg atgagatcgc aaatgcccgc tacatcgagc ttagtagtct cctctacccg
     2461 aatcccagcc tgagcaatgc cagacctatc cctccataga gactcctcta tggccttgcc
     2521 tattttgaat tcctcagcat acttgcgaat cagatcctgt tctgctggcg aaaaagcttc
     2581 catgtgcccc atcaaaatcg caatgaagtc ggcaacctgc tggctagtat agggctttac
     2641 agaagagagc cttgaaatga ctccgcgaag ctccgcggtt tcaatcatca tatagacagg
     2701 atgtgtaagc tcgaccgcta gatgtccctg tgcatacaca gccccggatc caccgctacc
     2761 catagcaaac tttaaaatca acagcgaaaa tattgccata gatattttgc caaaaccaaa
     2821 cctagaattc ttccggttca tcgctttatc ctaaaaatca ttctttgatg ttcgaaagca
     2881 ttttctagct ttccttgatc acataaccag aaaagatacg agcgaatggt agatccaaca
     2941 agtacatact ggttagcatt aaggactata tcataatgag aaaatacggc agccataaca
     3001 ttttcgagcg tagccccttc tgcacagttt gcctcggaat ttaccgccga gacaatgaca
     3061 tcagcaattt cgaaaatctt agcccgattt atatccacaa gtgattttat atcttgagtc
     3121 ggttctgcat gagctggtac aaaccattca gcctcgctcg actcgagcat cgaaagcgtt
     3181 gcaagttctg cctcaatatc atacaaaaag aaaaggccat atttttcaag aattggtgca
     3241 ccggcaagag aatctgcaac aaaaaacaca ttgtctgggg ttagaattcc aatcatgcta
     3301 aaataatggc ctggcagaga cataatctgc aaacaaggca tgccttgtgc cgcactctgt
     3361 gattcatttt tgggtatgat tgctgacgca cttgtaggag cgctccaagg cgcgaatgtc
     3421 gaagcgctct gcaattcatt tataggtgaa ccctgtgatg tatttgcagc tgcgctccca
     3481 tgtacctgtg cgggcttgga aagctgccac ctgccatctt tgtcgaaggt caaagcacaa
     3541 ggaggctcga gcaagcaatt tgctcgcgaa ggctgtgcca taagaaactt gttcctcagc
     3601 gctttctgtg gaaaactgcc agataaaaac gaaggctcaa gctgaggata gctcaaaaaa
     3661 gccgcctcca gctctgttgc tgcaatgcta caaccagttc gcttagacag gaatgcattt
     3721 ccaccgcagt gatccgcatt ggaatgagtg ttgataatca ttgcgagctc gacaccaagc
     3781 cgctcacatt cgcgcagaat cttccttcct gcatcctcgt cgccaccaga atctataaca
     3841 attgcgcgag cgacatcgct ctgcttgaaa acatagagtc caagattggt tggcccacgc
     3901 gcaacaagag tattgcccaa gaagggttcg aatagaattg gcataggtta ttgttactct
     3961 ttttattgtt cttcagcaag ctttctggct agcagggctc aaaaaaaagc cctcatgcaa
     4021 tgtcggcatc gggggtaggg ttggccgctg gggttggggg attagcggca caaaaaccga
     4081 caccgagcaa tcgcacgaag gcattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnncgtgt accatgcagt agtcagctat caaccttcaa atttgccttg agcgacaacc
     4261 ctatgcgccg ccgcaccgca tcgatgctga taactgtaaa gtcctttaca tcaccaacct
     4321 ttagcacctc cattggattc ttaacaaagt gctccgccat ctcagagaca tggataagtg
     4381 cgttctcttt tatgccgata tcgacaaagg caccaaaatc gacgacattc ttcacttttc
     4441 cttttactct catgcccggc cggagatcct cgaggctcaa cacaccttcc tgcagaattg
     4501 gcggcggaag gtcatctcgt ggatcacgat ttggtttctt tagttcgcta attatgtcat
     4561 ccagagtaat atcaccaatc ttatatttct gcttcaaact ctctctttct ttggtttgcg
     4621 gttccttgcc agacttcacc atggacaaaa tttctgaggc aactgcataa ttttcagggt
     4681 gaacccaagt attatctagc gcgttatcgc tctccggtat cttgagaaag ccggcacact
     4741 gctcgaacgt cttctcgcca agccctggaa tctcctttaa tagataacga ttccgtatca
     4801 cgccgcttgc atctcggaac ttgacaatct tccttgccaa gctcgcgttt atgcccgata
     4861 cataccgcaa tagcgaataa cttgcggtgt ttagattgac tccaacctgg ttcactaccg
     4921 atccaacaac atcgtcgagc tggctcgcca gctgtttctg gttgacatcg tgctgatata
     4981 gccctactcc aatgctcttt ggatcgattt taactagctc cgaaagagga tcctgcagac
     5041 gccgccctat cgagatcgcc cctcggattg taagatcgag atttggaaat tcttctctcg
     5101 caagatcact tgccgaatac acagacgcgc catcctcatc tacgactgca aagcggcatg
     5161 aaagcccatt ttctgcaatc gattccgcta tcagcttttg aacctcatgg cttccagtac
     5221 cattccccac cgctatgact gaaagcgcat attttatcac tgctgccgcg acatccttct
     5281 tgccctgttc gggcttggtc tcctggttta taacaaagta atccaagaac ttaccggttt
     5341 catcgagcgc tgcacatttt gtaccagttc gaattcccgg atcgatgccc aagactcttg
     5401 taccacgcaa gggcggctgc ataagaagat tgcgtagatt ggtgctgaac acctctattg
     5461 catggctttc cgcgctttcg gtgagatccg agcggatctc ccttcgcact gctggcaaaa
     5521 gaaggcgcaa aagcccgtcg attactgctt gcttatgctg tgcattggct ggtcgcacac
     5581 gctctagcat ccgcgcttcg actaattcat cgctcacctc tatcgacaca tcgagctcgc
     5641 cttctcgttc tcctcgattt atcgcaagca ctctatgatg cttgagagtc gagagaggtt
     5701 ctcgatagtc ccagtacatc tgataagtgg attgccctcg cttttcctca tcgcctatac
     5761 cttttaccgc aagctctcct tccttgataa atactcgttt tatgtgggcg cgcaagcttg
     5821 tatcctgcga aagccgttcg gcaaggatat ctcgcgctcc cgaaagcgca tcctcagcgc
     5881 tctccacgga aagctcgggg tgttcttcat ttcgatggat aaaagaagtg gcagcagcaa
     5941 ccacttcctc caggatggtg gttctttcca tcagctccgc aaaaggagct agtccctttt
     6001 cttgagcgag catgccgcgt gttttcttct ttttcttgaa aggtgaccag aggtcctcga
     6061 gttccgtcag agtagtagcg cgcagaatgt tctcatgtag aaaagcatcg agcttaccca
     6121 tagctgagat accttttatt acttcgagcc tgcgctcttc gagatttttg taactttgga
     6181 acttttggat acaatcgcga acttgtaact cgtcaagatt gccagttcgc tccttgcgat
     6241 atcgacttat aaagggaata gtgcatcctt cggctgaaag atccagtacc gcttgtactt
     6301 gcaaaagccg tatagcaagc tcatttgcta ttctctccgc aatcttaatc tcatctacgc
     6361 agagattctc tattctggtt gggtcaaaat ctattgtgtc atccatagcg agcctaaagt
     6421 tatgggaagt tcaaaaaaat atcaaggtca agattccagg catgatgatt cactggatct
     6481 ctaaccatat atggtgtatc cttgtaatga taactctctg cattgagctg aatataccgt
     6541 tatgaattaa agaggcacct gatggggaag ttcagcaata aggttcgaac attttccgcg
     6601 ctcgaagttg caaagctctg tggtgttgtt aatcaaacag cgataaattg gatacgaagt
     6661 ggctatctca aagcctttaa tacgcccgga ggacaatatc gagtttatgc cgaagatctg
     6721 gtttcttttc tcaaagaacg agatatgcgc gttccggatg agcttaaaga cgaccttact
     6781 atgccggtgg atagtggact cgcgataatc gtagatgatg acaaagatct caacacaatt
     6841 cttaaacgac tcctcgagcg aagaatagac gacctgcgcg tcgcacaggc ttacgatggc
     6901 tttgaggcag gccgaatcat cgcggagcgc cgtccggcta tagtcatctt ggatctgaat
     6961 cttcctggtg tagatggcct ttcgctctgc aaaaaaatcc gatcggatga gtcctttggt
     7021 cagccggcgg tgatagctat tacgggaatt ggagaagagg atgcccaacc tcgcatgatg
     7081 gaagctggtg ccgatgcatt cttttctaaa ccgctaaatt tcgatgcttt gatagcaaaa
     7141 acaaaggaac tcatggaagc ccgcaaagat ctagaaaaga aggatggcaa ttgaataaca
     7201 ttatattgaa aaagtccata tcgaagagga tagactccaa ttatgaaaga gctgtataga
     7261 tctgataatg gccgctgcat aattgcagtt ggagatataa ccaatatcgc ttgcgatgca
     7321 attgtcaacg cagcaaactc gagcctcctg ggagggggcg gtgtcgatgg agctatccat
     7381 agcgctggag gcccagcaat cctcgccgag tgtcgtgcgt tgcgaaacag ctccttacca
     7441 aatggattgc ctccgggaaa agcagtggca actaccgccg gtcagcttcc cgcaaggcgc
     7501 gttatccata cggtagggcc gatttggcaa ggaggggcct atggcgaaga cgaaacactg
     7561 gcgtcctgct atcgcgagtg ccttgcgctc gctgtgcacg agcagctcaa gtctattgcc
     7621 tttcctgcca tctcaaccgg tatctatgga ttcccaaaag aacgcgcagc caaaatcgcg
     7681 tggaatacgg tacaggatta tttagcgagt acccctgaag ctgcctccat aatagtatgg
     7741 tttgtattct tttcggagat agatgcgcat atctttcttg aggccaatgg aaaaataaag
     7801 ctatgaaagc cgcactattc acacaattta gagagcctat tgagatagtt gaggtgcctg
     7861 atccaatagc gcctgacgat ggcgtagttc tcaaagtcga tgcaacgggc atctgccgca
     7921 gtgattggca cggctggcag gggcacgatc ctgatataag gcttccacat gtgcccgggc
     7981 atgaactcgc cggcactatt gttgaagtcg gcagagatat caagaattgg aaactcagcg
     8041 agagagtgac tatgcccttt gttgctggct gcggccactg tccaccctgc cttagctcca
     8101 atcagcaggt ctgcgacaac caattccaac cgggattctc ggcgtggggc tccttcgcag
     8161 aatatgtggc tattcgctat gcagatatga atcttgtgcg cttgcctgag agcctcgatg
     8221 cagcaacggc ggcaagcctg ggctgtcgtt ttgctactgc tttccgggcg ctggaagcac
     8281 aatccagact tcgcgcggga gaatggatcg caatctttgg ttgtgggggt gtgggccttt
     8341 cagcgatcat gattgccaca gcgatgggcg ctcgaatcat cgcaatcgat attcaagaag
     8401 agaagcttaa aaaagccaag gaaattggcg cggatattgt aatcaatagc cgagaagtat
     8461 ctgatattgt tgctgtaatt cgcgatatca cacatggcgg cgtacaggtt tctatggacg
     8521 cacttggtag tccagagaca tgctttagcg ccatctcaag ccttgccaag cgcggtcggc
     8581 atgtgcaagt tggcctcatg ctcgctgacc acaaacatcc ccgaattcca atggacctcg
     8641 tgattgcgcg cgagctcgag atctacggga gtcatggcat tcaagcccac cgttatgatg
     8701 tgcttcttgg aatgatcgaa gctggaaaac tccacccaga gcggctcatc gaagctcgcc
     8761 ttaacctacg ccagggcgtt gattttttgc agaagatgga tcaattccca ggcgtgggca
     8821 ttagcgtgat cacatcgatg caagataatt cctagcaaga ttttgacaga attcccaaga
//
LOCUS       DAOOWY010000076         8791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_7952_length_8791_cov_9.415522, whole genome shotgun sequence.
ACCESSION   DAOOWY010000076 DAOOWY010000000
VERSION     DAOOWY010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7952_length_8791_cov_9.415522"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK105_06460"
     CDS             <1..498
                     /locus_tag="PK105_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU61390.1"
                     /translation="AFKELEKDGFVKIIPSIGCLVSSIGLDYIHEIFEIREILECAAA
                     RIVAERGDKSAFEEMLKNHESFELAEKKNIKGSVVSGFEIHTAIFETIGNSRLLDFYK
                     NIQNHIIRIRLFFIYQFDLDKTERMNAEHKMILKTIIAGDPNAAEKAMKEHLRNSQER
                     IKSLL"
     gene            512..1186
                     /locus_tag="PK105_06465"
     CDS             512..1186
                     /locus_tag="PK105_06465"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HOU61391.1"
                     /translation="MVHVLKIAERPDEHFIESFRKLAVATVYEASGRKGFIDCGIKPL
                     AKGMKICGPAFTVQCAPGDNLMLHKALQAAKAGDVLVASMGDGYFYGYFGSLMGTAAM
                     TAKLGGLAIDGCVRDAEELIDMKFPVFCRGVCIRGTTKNGLGTINYPLNFGGTTIYPS
                     DLILGDDDGMVVVRYRDLADVLQKSLDRVANEEKKAEQLRSGISSMELNKLEDVCIAL
                     GMKEEE"
     gene            1191..2240
                     /locus_tag="PK105_06470"
     CDS             1191..2240
                     /locus_tag="PK105_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOU61392.1"
                     /translation="MKICYRDLQNAASEILRHVGENEENAQIASEIMVVNDLRGVSTH
                     GTNLLRMIAKRCEAKMLSLPSRVTVVSDNMATLILDGGDGLGQVAGYKAMNAAIEKAR
                     LYGLGMALIRNTNNVGSLGYYVHKTAMQDMAAIMMTNGNPSMAPYGGAEPFLGSNPIA
                     FAVPSSSGMPIILDMSSSVVARGKIRMAALKNEEIPLDWALDSNGKPTSDPQKALKGC
                     LLPVGGPKGSGLAIIVDIFAGIMSGSSYGPNLKSFHELEGQTGVGACFIAIDISRFMP
                     VDQFKNLVGEYGEVLKSCKLREGFKEILLPGEIELRKEQESKESGIEMPEEVIDSLNE
                     LLRKAGIGFLLRSMN"
     gene            2282..3091
                     /locus_tag="PK105_06475"
     CDS             2282..3091
                     /locus_tag="PK105_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOU61393.1"
                     /translation="MKMRLGCFGYIKDLDLIAAAGFDCAELQVKEIMAMSEEEFKKAK
                     KTLKNCGITAEVFDNPIPLDCRISSPSFDLAYYRDFLEEISDRTAEMGARYIVFGNGK
                     ARSLPSEGDIASATSKFDDFFISLLEITAKRNITVLIEPLAKSLSNIVNSLSDAVEFI
                     KKYKMHNLKTLLDYRWMVEEGWPLTVIEDYELYIKHVHIDNPLSPFPTRIVPRLNDGF
                     DYAPFINTLKNIAYKEIISIEASVFQDFAKEIREGLGLFKANHIEPYRSLS"
     gene            3112..4152
                     /locus_tag="PK105_06480"
     CDS             3112..4152
                     /locus_tag="PK105_06480"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOU61394.1"
                     /translation="MKALVYYGPRDIRYQDVPTPKPRAGEVLVHVRAVSICGSDLAGY
                     KAVSSMRAPGLIMGHEFSGEIAQLGEGVTNLKVGDRVGVVTNLYCGKCQDCKEGRTNV
                     CDYRYIIGTTMKAGSYNGAMSEYVVAPAGKIMPLPDNVSFDEAALVEPLSISLRATKH
                     VANEIPNRTVAVLGVGPIGLMAIMCMKSFGAAKIIAIDLLRNRLELARKCGATDAIVS
                     NEDVPAIIRQMTNNVGIDVVFDAAGVPSTVNMAIEIARNAGSILMVGMASPTLEIEYK
                     HAVSKELKLQASYMYTTEMREGLQMIADGKLNVAQMITSRHTLSEGPKVFEELASGNT
                     KEVKVILFPEQN"
     gene            4186..4932
                     /locus_tag="PK105_06485"
     CDS             4186..4932
                     /locus_tag="PK105_06485"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOU61395.1"
                     /translation="MELEKKVAVVTGSGNGLGMGIAKKMFAEGASLALWDIDFESVEK
                     LAFELDPSGQRVLALKVDITDEAGVQAGVVKTVNRFGRIDVLVNNAGISRHKPLEETT
                     LEIWDAVIKVNLTGTFICCKAVAPIMKKQEFGKIVNIASLGGRTGRPGVGVNYAASKG
                     GVIGLTKLLAKELGPSNIYVNAICPGPILTDQTKQYPREVFDTWNAGRAVPKDGLPED
                     IGDAVVFLASRRSDWVTGISLDVNGGIFIG"
     gene            4957..5325
                     /locus_tag="PK105_06490"
     CDS             4957..5325
                     /locus_tag="PK105_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU61396.1"
                     /translation="MSGLKVVDINKVEPERKTPPRSSWILISEKTVGAQNLALGVNET
                     EPGSMVPEHMHETEEEVMFFLEGEGKFITQDQEIELKPGICVYNPPGALHKIVNTGTT
                     KLKFIWVYSPQLKSHRIPNS"
     gene            5347..6309
                     /gene="iolN"
                     /locus_tag="PK105_06495"
     CDS             5347..6309
                     /gene="iolN"
                     /locus_tag="PK105_06495"
                     /EC_number="3.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF041098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-scyllo-inosose hydrolase"
                     /protein_id="HOU61397.1"
                     /translation="MSEWEFPQEGQMDKPSKIYFQTMTKYDVEERLKTNDLILIPIGS
                     TENHGNAGPYGEDTFIVTRIAELVARKVGCTVAMPMWYGSHPFHHIGQPGTVPLSDDV
                     FTAHIRAMISGFWNAGFRKQIFLSMHGQEYALPSAIQEWGKKYQVPAIVIFLDLPRIM
                     GTDLKDKEHGGPFETPFQHADEAETSISMALFPELCQPEHAENTALKGFLPPGHVDRG
                     GDIYGNPIPGHCIIGGTGIECVTFPEGVLGKAKLADPSKAKKAIENVLNYMVKLHNDI
                     MERFPVGKLPDIKLMTQRDEREVELLLKGPLNGGKHIYTVAWPS"
     gene            6362..7396
                     /gene="dctP"
                     /locus_tag="PK105_06500"
     CDS             6362..7396
                     /gene="dctP"
                     /locus_tag="PK105_06500"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOU61398.1"
                     /translation="MKKLGLMVLLICIALSGAFGQGKVYELKLAHADPTDPAVSRKQA
                     QAIAFASMVNSRSGGRINVKIYGGGALGAEREYIEGIKAGTIEAGLASGPVADFFPPA
                     AITDIPYLFPTSKVAWDVMDGPFGDKLRAMFLKQTGMRCLAFGEVGFRHFTNSVRPIK
                     NPNDLKGLKIRVMETPVYVNMLKAAGANPTPIAWTETYTALQTHVVDGQENPIASIIM
                     AKLYEVQKYITLDGHSYGVDWFIINEKFFQSLPPDLQYIVMDTAQTVAAVGRGFITLQ
                     NAQGLQTLIDAGMQVYSPTDAEMQQFKKVMQPAVIDWLKTKVDPALINELLKAVDDAV
                     VAQKAKEGLK"
     gene            7535..8044
                     /locus_tag="PK105_06505"
     CDS             7535..8044
                     /locus_tag="PK105_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOU61399.1"
                     /translation="MKEQSLFMKITEKLESAVRWIVTILLAIMVVDIITAVFFRYVLQ
                     NSIFWAEELSRYLMIWAGMLGAGLAMKDDAHIGIDFVVRALPDSMQLTVKVIAKLVVE
                     IFLIVVFIESIDYLKTLSIQRSAALEIPMAIPYLSVTAGMIFMAIENILGIIRLFDDA
                     KSKGQEASR"
     gene            8041..>8791
                     /locus_tag="PK105_06510"
     CDS             8041..>8791
                     /locus_tag="PK105_06510"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOU61400.1"
                     /translation="MIPAIIAFAVFLFLGVPVSFSLGSSSLIYLLVEGKPIGLVAQRM
                     FTGIDSFPFMAIPFFVLAGELMNRAGISKRLIDLADVFVGRLRGGLGHVNVLTSMFFA
                     GITGSAVADTSSEGPIIIPAMVAAGYDADYAAAITCASSVIGPIIPPSIPFVTYSLVS
                     ATSVAALFMSGLIPGILLGVSQMIANAIISKKRNYPRSERWPSPKEIGRAIVRGIIPL
                     FMPLIILGGIIGGIFTPTEAAAVAVLYALLIS"
BASE COUNT         2526 a         1740 c         2210 g         2315 t
ORIGIN      
        1 gcctttaagg aactggaaaa ggatggattt gttaagatca taccgagtat aggatgcctc
       61 gtttccagca taggattgga ttatattcat gaaattttcg aaattcgtga aatactagaa
      121 tgcgcagcag ctcgcattgt tgctgagcga ggagacaaat cggccttcga ggagatgctc
      181 aaaaatcatg aatcattcga gctagccgaa aagaaaaata tcaaaggttc tgtggtttct
      241 ggatttgaaa ttcataccgc gatttttgag actatcggaa attctcgact tctggatttc
      301 tataaaaaca tccaaaatca cataatacgc atacgcctat ttttcatcta tcaattcgat
      361 ctcgacaaaa cagagagaat gaatgctgaa cataagatga ttttgaagac gataattgca
      421 ggcgatccca atgctgctga aaaggcaatg aaagagcact taagaaatag ccaggaacgt
      481 atcaaaagtc tgctctaaga aggagggcaa catggttcat gttctaaaaa ttgccgaaag
      541 accagacgaa catttcattg aatcatttag aaaacttgca gttgcaacgg tctatgaggc
      601 ttctggaaga aaaggcttta tagattgtgg tataaaaccg cttgcaaaag ggatgaaaat
      661 ctgcggtcct gcttttacgg ttcagtgtgc tccgggagat aatcttatgc tacataaggc
      721 attacaagca gcaaaagctg gcgatgtgtt agttgcaagc atgggagatg gatattttta
      781 tggatatttt ggttcgctga tggggacagc tgcaatgact gcaaagcttg gaggccttgc
      841 tatagatggc tgcgttcgcg atgcagaaga gcttatagat atgaaatttc cagttttctg
      901 ccgcggagtg tgtattagag ggacgaccaa aaacggcctg ggaacaatca attatccact
      961 caattttggc ggaacgacta tttatcctag cgacctaatc cttggcgatg atgatggtat
     1021 ggtggtagtt cgatatcgag acttggcaga tgtccttcag aaatcgctcg atagggtggc
     1081 gaatgaagag aagaaggcag agcagcttcg aagcggtata tcaagcatgg aactaaataa
     1141 gcttgaagat gtctgtatcg cactcggtat gaaagaggaa gagtaaggct atgaaaatct
     1201 gctatcggga tctacagaat gcagcgtccg aaattctaag gcatgtcgga gagaacgaag
     1261 agaacgcgca aatagcctct gaaataatgg ttgtcaatga tctgagagga gtaagcacac
     1321 acggtacaaa tctacttaga atgatagcaa aacggtgcga agcaaagatg ctcagtcttc
     1381 cttccagagt tactgtcgtt tccgacaata tggcgacttt gatattggat ggcggagatg
     1441 ggcttggcca ggttgctggc tacaaggcta tgaatgctgc tatcgaaaaa gcaaggcttt
     1501 atggtcttgg tatggcgctc ataagaaata ccaataatgt gggttcgctc ggttactatg
     1561 tccataagac tgctatgcag gatatggcag ccatcatgat gacaaatggg aatccttcga
     1621 tggcgccgta tggaggagcc gaaccttttc ttggatcaaa tccgattgcg tttgcggtgc
     1681 cttcttcttc tggaatgcct attatcctag atatgtcgtc ctcggttgtg gctaggggaa
     1741 agatcaggat ggctgccctc aagaatgagg aaataccttt agattgggct ctagatagca
     1801 atggtaagcc aacaagcgat ccacaaaaag ctctcaaagg atgtcttcta cccgtggggg
     1861 gcccgaaagg gtctgggttg gcaatcattg tggatatttt tgctggcata atgtcaggct
     1921 catcctatgg gccaaatttg aagagcttcc atgaattgga aggccaaact ggtgttgggg
     1981 cttgcttcat tgccatcgat atttcgcgct ttatgcctgt ggatcaattt aagaatctag
     2041 tcggagaata tggcgaagtc ttgaaatctt gcaaacttcg cgagggcttc aaagaaatcc
     2101 ttctgccagg agaaatcgaa ctaagaaaag aacaggaatc aaaggaaagc ggtatcgaga
     2161 tgccagaaga ggtaatagat agcctaaatg agctgttacg aaaagcagga attggctttt
     2221 tgctgagatc gatgaattga tgcctaaaaa ggatggaagg ggcgtttaaa aggaaaagat
     2281 gatgaaaatg cgacttggtt gttttgggta tatcaaagat ttggacttga ttgcagcagc
     2341 tggctttgat tgtgccgagt tgcaagtaaa agagataatg gccatgagcg aagaggaatt
     2401 caaaaaagcc aagaaaacgc tgaaaaattg tggaataact gctgaagtat tcgataatcc
     2461 tattccttta gattgccgca tttcatcacc ttcctttgat ttagcttatt atcgagattt
     2521 tctagaagag atctccgatc gaacggctga aatgggagcg cgctatattg ttttcggaaa
     2581 cggtaaagcc cgaagccttc catcagaggg agatattgca tccgctacca gcaaattcga
     2641 tgactttttc ataagtcttc tcgaaataac agcaaagcgg aatatcacgg ttctaataga
     2701 gccgctggct aagagtctat cgaatattgt gaattcgctt tctgatgcag tggaatttat
     2761 aaagaaatat aaaatgcaca atctcaagac tcttttagac tatcgctgga tggtagaaga
     2821 gggttggcct ctcactgtta tcgaagatta tgagctctat atcaagcatg tgcatatcga
     2881 taatcctctt tcgccatttc cgaccaggat tgtaccgcga ctgaatgatg gctttgatta
     2941 tgctcctttt ataaacacgc tcaagaacat tgcctataaa gaaattattt caatcgaggc
     3001 atctgtattt caggactttg ctaaggagat aagggaaggg ctaggcctat ttaaggctaa
     3061 ccatattgag ccatatcgta gcctttcttg attctcgtaa ggaggcattt tatgaaggca
     3121 ttggtatatt acggacctcg cgatattcga tatcaagatg ttccgacgcc aaagccaaga
     3181 gcaggtgaag ttctggtaca tgtcagagct gtctctattt gcggctcgga tcttgcaggc
     3241 tataaggctg tttcctctat gagggcaccg gggctcataa tgggacatga gttttctggt
     3301 gagatagctc aactaggaga aggagtaact aaccttaaag ttggagatcg tgtcggtgtt
     3361 gtcacgaacc tctattgtgg aaagtgccaa gattgcaaag aaggcaggac aaacgtgtgc
     3421 gattaccgat atatcatcgg tacgacaatg aaagccggaa gctataacgg cgcaatgtcg
     3481 gaatatgtcg tcgctcctgc ggggaaaata atgcctttgc cagataatgt ctcattcgac
     3541 gaagctgctc ttgtcgagcc tttgtcgata tcgcttcgag cgaccaaaca tgtagccaat
     3601 gaaataccaa atagaacagt agcggtacta ggagtggggc ctattggttt aatggctatt
     3661 atgtgcatga agagctttgg agccgcaaaa atcattgcaa tcgaccttct tcgaaatcgg
     3721 ctagagcttg ctcgcaaatg tggtgcaacc gacgcaatag tctctaatga ggatgtacca
     3781 gctatcattc ggcagatgac aaacaatgta gggatcgatg ttgtctttga tgccgcagga
     3841 gttccttcga ccgtcaatat ggccatcgaa atagcaagaa atgcgggaag catcctcatg
     3901 gtcggcatgg cctcgcccac gctggaaatt gaatataaac atgcggtctc caaagagctg
     3961 aaactgcaag ccagttatat gtacacgacc gaaatgagag aaggcttgca gatgattgcg
     4021 gatggcaaac tgaatgtggc tcagatgatc acttcgcgac ataccctttc tgaagggcct
     4081 aaggtgttcg aggaacttgc ttcaggcaat accaaagaag taaaggtcat tttatttccg
     4141 gagcaaaatt gagaaagatg cagataagca aagaggctag agaatatgga acttgaaaaa
     4201 aaggttgcag ttgttacagg ttcgggcaat ggtctcggca tgggcatagc aaagaagatg
     4261 tttgctgaag gcgcaagtct ggccttatgg gacatcgact ttgaatctgt agagaaattg
     4321 gcttttgagc tggatccgag cggacagagg gtgctcgccc taaaagtgga catcaccgat
     4381 gaagccggtg ttcaggccgg agtagtcaag actgtgaata gatttggccg catagatgtt
     4441 cttgtcaata atgctggcat ttctcggcac aagcctttgg aagagactac gctggaaatc
     4501 tgggatgcgg ttatcaaggt aaacctaacg ggaactttta tatgctgcaa ggctgtcgct
     4561 cccatcatga agaagcaaga gttcggaaag attgtcaata ttgcttcgct tggaggcagg
     4621 acaggaaggc caggcgtcgg cgtcaactat gctgcttcca agggaggagt aattgggctt
     4681 accaaattgc ttgcaaaaga actcggacca tcgaatatct acgtcaacgc tatctgtcct
     4741 ggtcctatat taacagatca gacaaagcaa tatccaagag aagtattcga tacatggaat
     4801 gcggggagag ccgttcccaa agatggattg ccagaagata ttggcgatgc agtggtattc
     4861 ctggcatcca ggcgttctga ttgggttaca ggtatatctc tggatgtaaa tggcggcatt
     4921 tttattggct aagaaccttt tagtatggag gagagaatga gtggattgaa agtggtggac
     4981 atcaataagg tggagccaga aagaaagacc cctccaagat cttcttggat cctaatttca
     5041 gagaagaccg tgggggcaca aaatttggct ttaggtgtaa acgagactga gcccggctcg
     5101 atggttcctg aacatatgca tgagactgag gaagaggtta tgttttttct ggaaggcgaa
     5161 ggtaaattca taacccaaga tcaggaaata gaattaaaac caggaatatg tgtgtacaac
     5221 ccgcccggag ccctgcataa gattgtgaat acagggacga cgaaactcaa gtttatctgg
     5281 gtctattcac cacagctaaa gagtcacagg attccgaata gttaggaaat gaaaggagaa
     5341 cacaagatgt cagagtggga attccctcaa gaaggccaga tggacaagcc ttcaaaaatc
     5401 tattttcaga ctatgacgaa atacgatgtg gaagaaagat tgaaaacaaa cgatctaatc
     5461 ctcatcccca tagggtcgac tgaaaatcat ggaaatgcgg gaccgtacgg cgaggacacc
     5521 tttattgtaa ccaggatagc ggagctggta gccaggaaag ttggttgtac ggtagccatg
     5581 cctatgtggt atggttctca tccttttcat catattggac agccaggaac cgtacccctt
     5641 tctgacgatg tatttacagc tcatattcgc gcaatgatat ctggattctg gaacgctgga
     5701 ttccgcaaac agatattcct cagcatgcat ggtcaagaat atgcgcttcc atcggcaatt
     5761 caagaatggg gaaagaaata tcaggttccc gcaattgtga tttttcttga tctcccgaga
     5821 atcatgggga cggatctgaa agataaagag cacggtgggc ctttcgagac gccattccaa
     5881 catgccgatg aggcggaaac atcgatttcg atggctttgt ttccagagct ttgccaacca
     5941 gaacatgccg aaaatacagc attgaaaggt tttcttcccc ctggtcatgt cgatagagga
     6001 ggagatatat acggtaatcc catacctggc cactgcataa taggcggaac aggcatcgaa
     6061 tgtgttacgt ttccggaagg cgttctggga aaggcaaaac tcgccgatcc gagcaaagca
     6121 aagaaagcca tcgaaaatgt cttgaattat atggtcaagc tccacaatga catcatggag
     6181 cgtttccctg ttggcaagct accggacatc aagcttatga cacagagaga tgagagggaa
     6241 gtcgaactcc ttctcaaagg accgctaaat ggaggaaagc acatatatac agtagcctgg
     6301 cctagctaga tatttggaag aatgaagcgt ggcttcatca taaaaggaag gaggtattcc
     6361 tatgaaaaaa ttgggattga tggttctcct gatatgcatt gcgctatcag gagcttttgg
     6421 acagggcaag gtgtacgagc taaaactcgc acatgcagac ccaaccgatc ctgcggtctc
     6481 cagaaagcaa gcacaggcta tcgcattcgc aagcatggta aactctagga gcggtggacg
     6541 tatcaatgta aaaatctacg gaggaggcgc tcttggagct gagcgtgaat acatcgaggg
     6601 aatcaaggct ggcaccatag aagctggtct tgcttctggg cctgtagcgg attttttccc
     6661 tcccgctgca atcaccgata ttccttatct tttcccgaca agcaaggtag cctgggatgt
     6721 tatggatggg ccattcggtg acaaacttcg agccatgttc ctcaagcaga ctggcatgcg
     6781 ctgcttggct tttggtgagg taggttttag gcattttaca aatagtgttc gtcctatcaa
     6841 gaatccaaat gatctaaaag ggctgaagat ccgcgttatg gagactccgg tttatgtcaa
     6901 catgcttaag gccgctggcg caaatcctac gccgatagca tggaccgaga cctatacggc
     6961 tctccagact catgttgtgg atggacagga gaacccaatc gcttcgatca tcatggcaaa
     7021 gctctatgaa gtgcagaaat acatcacact cgatggccac agctatggag ttgactggtt
     7081 tattattaat gagaaattct ttcagagctt gccgccagat ctacaataca tagttatgga
     7141 caccgcacag acagttgctg cagtaggtcg tggctttatc acactccaga atgcccaagg
     7201 tctccagact cttatcgatg cggggatgca ggtttactct ccaaccgatg ccgagatgca
     7261 acaattcaag aaggtcatgc agccagcagt catagattgg ctcaagacca aagtcgatcc
     7321 ggcgcttatc aatgaacttc tgaaagccgt agatgatgcc gtagtagctc agaaggcaaa
     7381 agaaggactc aaataagcag tctgtagatt ggatgcgcag gggcgaatcg aacagcgtct
     7441 ctgcgccttt ttgttaatat tctatgaatg ttttttatca ccgcatcctt tctaggatta
     7501 tgtttaattc ttatgggaaa ttaaggggca aaggatgaag gaacaaagtc tatttatgaa
     7561 aatcaccgaa aaattggagt ctgcagttcg atggatcgtc acgattttgc tagccataat
     7621 ggttgtcgac attattaccg cggtattttt taggtatgtc ctgcaaaatt cgattttttg
     7681 ggcagaggaa ttgagcagat atttgatgat ctgggctgga atgcttggag cgggccttgc
     7741 tatgaaggat gatgcccata ttggaattga ttttgtggtt cgggcccttc ccgattctat
     7801 gcaactcaca gtcaaagtga ttgcaaaatt agtcgtggaa atatttttaa tcgtggtttt
     7861 tatcgagtct attgattatc taaagactct ttctattcag cgctcggcgg ccttggaaat
     7921 tccaatggct ataccttatc tttcggttac cgccggtatg atattcatgg caatcgaaaa
     7981 tattttagga ataattcggc tctttgatga tgcaaaatca aagggacagg aggctagtcg
     8041 atgataccag ccattatagc ttttgcagta tttctattct tgggggttcc tgtctccttt
     8101 agcctaggct cttcaagttt gatttatctt ttggtagagg ggaaacctat tgggcttgtg
     8161 gcacaaagaa tgttcaccgg catagattcg tttccattta tggctattcc tttctttgtt
     8221 ttagcaggcg agctcatgaa ccgcgcggga atttccaagc gattgataga tctggcagat
     8281 gtctttgtag gaaggctcag gggtggactt ggacatgtca atgtgcttac ctcgatgttc
     8341 tttgctggga taacgggctc tgccgtggca gatacttctt ccgaaggccc tataatcatt
     8401 ccagccatgg tagcggcagg ctatgatgcc gactatgcag ccgcaataac ttgcgcttct
     8461 tcggtcatag gacctatcat cccgccaagt atcccattcg tcacttattc tctcgtgagt
     8521 gcaacctctg ttgctgccct ttttatgtct ggtcttatac caggcatact ccttggcgta
     8581 agtcagatga ttgcaaacgc aattatatct aagaagagaa attatccacg ttctgagcgt
     8641 tggccaagtc ccaaagaaat aggaagagca attgtccgag ggatcatacc gctttttatg
     8701 ccgctcatca tattgggagg cataatcggt ggtatcttta cccctacgga agcagcggcc
     8761 gtagccgtcc tttatgctct attgataagc c
//
LOCUS       DAOOWY010000077         8594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_8175_length_8594_cov_13.399344, whole genome shotgun sequence.
ACCESSION   DAOOWY010000077 DAOOWY010000000
VERSION     DAOOWY010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8175_length_8594_cov_13.399344"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..2958
                     /locus_tag="PK105_06515"
     CDS             370..2958
                     /locus_tag="PK105_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOU61401.1"
                     /translation="MEAKKKTHPKASVVVAKKPAARRGSSTSIKASARSSALRSTRGI
                     IALIFFIVALFLFISMLLWSYFPTIWRPLVLPGQTLLNIFSWAAYYLPLWLAWAAILV
                     YVPSFCPRMTYLLGTSALPFVVATGFARLSANPGSFFEHHPSMAKVGLSSLYAALGFL
                     LAASLMIVIAGYIKLSEWLRANGFIKTRADKKKNGEEQSFGQRLATFIKDNSLKREER
                     RKRKEEARLARARALAEKDISLNIPDVPPPDPLKSEKLGGFYASGTSRAGSEMVQKDL
                     GATSVPMGSAIGSTGLSLGSRSLGPIAGFDTGGISFTSVPPARPAQSEEEHKRAIVER
                     ILERKAAKPYQVPIDGLLNNYPDGQYWIIDEKTRASASVLKETLAEFGIEAEVTGIRK
                     GPVITMFEILPAHGVKISKITNLSDNIALRLAASSVRIVAPIPGKHAVGIEVPNERRA
                     IVSLRELIESEKFRQSKMEIPVALGKDIAGEVQLIDLTQMPHILIAGATGSGKSVCVN
                     SIILSILYKRTPEEVRLMLIDPKIVELKLYNDIPHLLTPVVTEAKKAFQALQYCICEM
                     ERRYSLLDNLGVRDIRSYNRRIRERNLAQDKVPYIVVIIDEFADLMQTTGKELEATLA
                     RLAAMSRAVGIHLVLATQRPSIDVITGLIKANIPSRIAFMVASKFDSRTIIDMVGAEK
                     LLGRGDMLFSGAQDPFPVRMQGAFVSEEEVERVVAHVKTLGEPEYIDEEIFVDEEESE
                     EPTLFDEEGSDPLFEKALEIVLQQGKASVSYIQRRLKIGFNRAARLVEMMEEKGIVGP
                     AQGSKPRDVLRNPGVFESSSGYEGIRKDASPDIGQIAGTSGSPSINPSLNPSPRRTFR
                     QDDQDN"
     gene            complement(3187..3894)
                     /locus_tag="PK105_06520"
     CDS             complement(3187..3894)
                     /locus_tag="PK105_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61402.1"
                     /translation="MLEIRDLSVYYGGIHALQGISLDVPDGVVVTMLGANGAGKTSTL
                     RTILGLVHPKEGTIKFNGTSLLGKRPYQIVKMGVAMAPEGRRIFPDLSVWENLLMGAY
                     SRTDAEGIKKDLEWVLGLFPRLAERKSQAGGTLSGGEQQMLAVARAVMAHPKLLLLDE
                     PSLGLAPVLITELYRAFAKIHKEGRTILLVEQNARAALSLANYGYVLETGRIVAQGSR
                     EELLCSDLVKRAYLGIA"
     gene            complement(3878..4672)
                     /locus_tag="PK105_06525"
     CDS             complement(3878..4672)
                     /locus_tag="PK105_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61403.1"
                     /translation="MEKGKPILNLDHLSMRFGGLLAVNDFSLTITSGELLGLIGPNGA
                     GKTTIFNMITGYYPPTQGQVIFEGQDITGFPPAKVTLLGIARTFQNIRMCKNMSALDN
                     VLISFHPHLRSSVFGVIFPFFGYNREEQKMRQEGMDLLARVGLTQWANERAGSLPYGL
                     QRQVEIARALATHPKLLLLDEPAAGMNPKEAGGLMEFICQIQEEFNLTVFLIEHVMQV
                     VMGICPRIRVIDFGVSIAEGCPDEIKANKKVIEAYLGEEELSAGNT"
     gene            complement(4672..5709)
                     /locus_tag="PK105_06530"
     CDS             complement(4672..5709)
                     /locus_tag="PK105_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61404.1"
                     /translation="MKRYRNLVLTLIAIALVGVFVWWGDKPNNLDPLIWRILNLGAIY
                     AIAAVGYTVINGIAGQFSLGPHGFMLLGGYMVALLMLPLKQKAVVWLFKGKELIWPFS
                     SFTLPSWGFILALLAGGVLAAVAALIVGIPALKALRGDYLAIATFGFGEILFVLACNL
                     IPLTNGPMGIKGIPHITNLAWSFGALVVVTYAALRIKNSSYGRALRAIREDEIAAESM
                     GINVFRLKMLAFIFSAFCAGVAGGLMGTLIGTVTPDTFKFFMTFYLLIIIVLGGLGSI
                     TGAVVTGFIFAGLFEALRVLDTATVEGMRMVFFAILLIGLMLFFRRGLFGEAEFSWDW
                     LFRGSSKLGRK"
     gene            complement(5706..6599)
                     /locus_tag="PK105_06535"
     CDS             complement(5706..6599)
                     /locus_tag="PK105_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61405.1"
                     /translation="MVFYVQQLFNGITLGSLYALIAIGYTMVYGILRLINFAHGDIFM
                     WAGYWVFFSFFLFHLPWWIGAILAVVLTAGMGMAIERFAYRPLREAPRISLLITAIGA
                     SFLLENLIIVLPWAGGRQKSFPRPEFMSQIFSLGTLRIQALTVWIPLIGLACLIGLLF
                     LIYHTKIGRAMRAIATDIEATEIMGADINKIISYTFLVGSALAAVGGILWGCRYPRLQ
                     WDLGITPGWRAFTAAVVGGIGNVTGAMIGGFALGLFEILLVAFIPNLSGYRDAFIFGI
                     LIIFLLVRPTGILGKPLKEKV"
     gene            complement(6660..7817)
                     /locus_tag="PK105_06540"
     CDS             complement(6660..7817)
                     /locus_tag="PK105_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61406.1"
                     /translation="MRKLVLGLIIVNLLLGTVVFAQEPIKIGVLVEFTGACAAYGQMG
                     RDALELARAKGIVPVQVLGRPIELVYFDARTEPTEATNGATRLINTEKAVAIIGTMCS
                     GPYLAAAEICQASGVPIIGPTTTNPLCTQVGDYCFRACFTDNDQGPLAASIAQKRYKA
                     KTAAVVIDMAQAYCVGLGQFFMDAFKALGGKIVAELYCRSGDVDYTAQLTEIQRKNPD
                     IIYTPNYYTEVAMMARQARDLGMMQPILGADGLYAPELIKIGGDAVEGLNFLTFWSAE
                     VTPNEASKKFVKEFRAKFGMDPDAFGALTVDCYMMIVDAIKRAGSANPAAIKQALKTS
                     DLDVITGHVIMTPKNDPKKDFAFLTVKEGKFSLVETIPVEQAWEIRALIGK"
     gene            8073..8495
                     /locus_tag="PK105_06545"
     CDS             8073..8495
                     /locus_tag="PK105_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61407.1"
                     /translation="MGLRMKDRQSLTRKIVPRYQKARKAEKEKILDEFVKNTGYSRNY
                     AIHILATWGEVHTLVIDEKPIRVVVGVSWTRKKRKENVHYTISPATIDRLLKVERKKL
                     EIKGRSHTKGGVLLKHQIPISTFYDWYERKPGFFTRGS"
BASE COUNT         2304 a         2196 c         2109 g         1985 t
ORIGIN      
        1 cttcgagttc tcgttccagc cctgggatta tgttatcatt gccatgaaga tacacaaggg
       61 gttcgcttcc ctcagatgag tctatgatct tgccgatatc gtcgctgaga gaatagtcaa
      121 tagtaacgac gcgatctttt ctgatttcca ttacaggtcc ttttaaaata gtcaaaatac
      181 tttcatcgca tttagaatgc gcttcctttg agcacgaatc gcgtacaatc gatacataag
      241 gacggtcatg ctctaggcag ggctatagta taatgaatta ttatattgcg cggcaatgga
      301 gcaaggcatc gaaatattcg taactggcac attccaaaat gccgataata attaaggatt
      361 tatctgccca tggaagcaaa aaagaagact cacccaaaag cctcggttgt cgtggccaag
      421 aaacctgctg cacgccgtgg ttcatcaacc tcgataaaag cttctgcaag aagttctgct
      481 cttaggagca ctcgaggaat aatagcgctc atatttttca ttgtagcgct ttttctgttt
      541 atttctatgc ttctatggtc ctattttccg acaatttgga ggcctttggt cttaccgggg
      601 cagacattgc tcaacatatt ttcttgggca gcctactatc tgccattatg gttagcttgg
      661 gctgcgatcc ttgtatatgt gccaagcttc tgtccacgaa tgacctatct attaggcact
      721 tcggcattgc catttgtagt ggcaaccgga ttcgctcgtc tgtctgcgaa tcccggttct
      781 ttttttgaac atcatccgtc gatggcaaaa gtaggattgt cttcgctata tgcagctcta
      841 gggttcttac ttgcggcaag cctgatgatt gtcattgctg gctacattaa gctctcggaa
      901 tggcttagag ctaatggatt tatcaaaaca cgcgccgaca agaagaaaaa cggagaagag
      961 cagagctttg ggcaaagact ggcgactttt atcaaggata attcgctaaa aagagaagag
     1021 cgacgcaagc gcaaagaaga agcgcgcctc gcgcgcgcaa gggcgctggc agaaaaagat
     1081 atctcgctca atattcctga tgtgccaccg ccagaccctc taaaatccga aaagcttgga
     1141 ggattctatg cttcgggcac aagtagggca ggctctgaaa tggtgcagaa agacttaggt
     1201 gcaacctctg tccccatggg atccgctatt ggatccacag gattatcttt aggatcacgc
     1261 tccttaggtc ctattgctgg tttcgacaca ggaggcattt cttttacctc cgtccctcca
     1321 gctcgtcctg cgcaatccga ggaggaacac aagcgcgcta tcgtggagcg aatactagag
     1381 cgtaaggcag cgaaaccata tcaagtacca atcgatggac ttctcaataa ctatcccgat
     1441 gggcagtact ggatcatcga cgaaaagaca cgtgcaagtg ctagtgtgct caaagagact
     1501 ctcgccgagt ttggaataga agccgaagtg acaggcattc gcaaaggacc tgttattaca
     1561 atgttcgaga ttctcccagc gcatggcgtc aagatttcca agattaccaa tttgagcgac
     1621 aatattgcct tgagacttgc agcctcaagc gtacgtatcg tcgcgcctat tccaggcaag
     1681 catgctgtag gaatcgaggt gcccaacgag cggcgtgcaa tagtctctct ccgggagctc
     1741 atcgagagcg agaaattccg ccagagcaaa atggaaatac cggtcgcact gggcaaggat
     1801 atagccggag aggttcagct tatcgacctt acccagatgc ctcatatttt gatcgcgggc
     1861 gccactggct cgggcaaatc tgtctgtgta aactcgatta tcttatcaat tctatataaa
     1921 cgcacgcctg aagaagtaag gcttatgctc atcgatccaa aaatcgtcga actaaagctt
     1981 tacaacgata ttccacatct cttgacacca gttgtcacgg aagcaaaaaa agcctttcag
     2041 gctctgcagt actgcatttg cgagatggaa cggcgctatt ctctgcttga caacctgggt
     2101 gtacgagaca tccgctctta taaccgccga attcgtgagc gcaatctggc ccaggacaaa
     2161 gtgccctaca tcgtagtaat catcgacgag tttgctgact tgatgcagac aacgggcaaa
     2221 gagctcgaag ccacgcttgc tcgtcttgct gccatgtctc gcgctgttgg aatccacctt
     2281 gtactagcta ctcagagacc cagtatcgat gtcataactg gtctcataaa agccaatatc
     2341 ccatcacgca tcgccttcat ggtcgcaagc aaattcgata gccgcactat catcgatatg
     2401 gttggggcag aaaaactcct tggtaggggc gatatgctct tctcaggcgc ccaggatcca
     2461 tttcctgttc gaatgcaagg cgcattcgtc tccgaagaag aagtcgagcg agttgttgct
     2521 cacgttaaga ctcttgggga gccagaatat atcgacgaag aaattttcgt cgatgaagag
     2581 gaatccgaag aacccactct ctttgatgaa gagggttccg atccactttt cgaaaaagca
     2641 ctcgagattg ttcttcagca gggtaaagct tcagttagtt atattcagcg acggctaaag
     2701 atcggcttta atcgtgcggc taggctcgtt gaaatgatgg aagaaaaggg tattgttggc
     2761 cccgcacagg gctccaaacc tcgtgatgta cttcgaaatc ctggagtgtt cgaaagctca
     2821 agtggatatg aagggatacg caaggatgca agcccagata tagggcaaat tgccggcaca
     2881 agtgggagtc cgagcataaa cccaagccta aacccaagcc cacggcgcac attcagacag
     2941 gatgatcagg ataactaagg ataagacacg atacaggtag cgcgcgacgc gtggaattgg
     3001 catgcagagc aagatgatag atcgattcac aatgaagtta tcgcattttt gcaaccaaaa
     3061 taaacctatc gccaactata tggccttttg agatagaaca gggaaagttt gctcgtcact
     3121 ttgcaggatc agagtcggtt ttgagaaaat cactcgttta tgcgagcgta tttctgggat
     3181 agtatactag gcgatgccca agtatgcgcg ttttaccaga tctgaacaga ggagctcctc
     3241 ccgggagccc tgggccacga tcctccctgt ctcgagcaca tagccataat tggcaagaga
     3301 aagggctgcc cgagcattct gctccacgag caggatggtc cgaccttcct tgtggatctt
     3361 cgcaaatgct cggtagagtt ctgtgatcag gaccggagcc aggcctagag agggctcatc
     3421 caagaggaga agtttggggt gggccatgac agcccgcgcc actgcaagca tctgttgctc
     3481 gccgccagat aaagtccctc cagcttggct ctttcgttca gcgagtcggg ggaaaagccc
     3541 taacacccat tccaaatctt tcttaatccc ctccgcatca gttcgggagt aggcccccat
     3601 gagcaggttc tcccacactg agagatcagg gaaaatccgt cgtccttccg gggccatggc
     3661 tacccccatc ttcacgatct ggtacgggcg cttgccaagg agtgaggtcc cattgaactt
     3721 tattgtgccc tccttcggat gcaccaggcc aaggatcgtt cgcagggtac tggtcttgcc
     3781 agcaccattg gccccaagca tggtcaccac taccccatca ggcacgtcca gtgaaatgcc
     3841 ctgtagggcg tgaatcccgc cgtaataaac ggaaaggtca cgtatttcca gcacttagct
     3901 cctcctcccc aaggtaagcc tcaattacct ttttgttggc ctttatctcg tctgggcatc
     3961 cttcggcaat ggagaccccg aagtcaataa ctcgtatgcg agggcagatt cccatcacca
     4021 cttgcatcac atgctcgatg agaaagacag tgagattaaa ttcctcctga atctggcaga
     4081 tgaattccat aagccctccg gcttctttgg gattcattcc cgctgccggc tcatccaaga
     4141 ggagcaattt gggatgtgtg gccagtgccc gggcgatctc cacttgtcgc tgtagaccat
     4201 aggggagaga cccagcccgc tcattcgccc attgtgttag ccccacccgg gcaaggaggt
     4261 ccattccttc ctggcgcatt ttttgctcct cacggttgta accaaagaat ggaaatatga
     4321 ccccgaacac tgaggagcgc aaatgaggat ggaatgatat aagcacgtta tccagcgctg
     4381 acatgttctt acacatacga atgttctgaa aggttctggc aatgccaagc aaggtgacct
     4441 tggccggggg aaaaccggtt atgtcttggc cctcgaagat cacctggcct tgggtaggtg
     4501 ggtaatagcc agtgatcatg ttgaatatgg tggttttgcc ggcaccgttt ggaccaatga
     4561 gcccaagtag ctccccggaa gttatggtca gggaaaagtc gtttaccgct aacaggccac
     4621 caaaccgcat actgagatga tcaagattga ggatcggctt gcctttttcc attatttcct
     4681 ccctagcttg ctagaacctc ggaacaacca atcccaggag aattccgctt cgccaaacag
     4741 cccccgccgg aagaagagca tgagcccaat caggaggatg gcaaagaaca ccatgcgcat
     4801 accctcgacc gtggcggtat ccaagacccg caacgcctca aatagcccgg cgaagataaa
     4861 cccagtcacc accgccccgg taatgctccc caacccacca agcacaatga taatgagtag
     4921 gtaaaaggtc atgaagaact taaatgtgtc aggagtcaca gttccgatga gggtgcccat
     4981 caatcctccc gccactccgg cacagaaggc agaaaagatg aatgccaaca tcttgaggcg
     5041 aaatacattg atccccatag actcggcggc aatttcgtcc tctcgaatgg cgcgtagcgc
     5101 tctcccgtag ctagaatttt tgatacgcaa ggctgcgtat gtcaccacaa ccagcgcccc
     5161 aaatgaccat gccagattag taatgtgggg gatgcccttg atccccatag gaccgttggt
     5221 caagggtatg aggttacagg caaggacaaa caggatttcg ccaaaaccga aggtggcgat
     5281 ggcaagataa tctcctctaa gggccttgag ggccgggatg ccaacgataa gcgcagccac
     5341 agccgccaat actccgccag caagcagggc taggatgaac ccccaagaag gcaaagtgaa
     5401 gctggagaat ggccagatga gctcttttcc tttgaatagc cataccactg ctttttgttt
     5461 caacggaagc atcaacaatg caaccatgta cccgcctagc agcatgaacc cgtgtgggcc
     5521 caaggagaac tgaccggcaa tcccatttat cacggtatat cccactgcag cgatggcgta
     5581 gatggcgcca aggtttagga tccgccatat taagggatca agattgttcg gtttgtctcc
     5641 ccaccagacg aaaactccca ccagggcaat agcaattagg gtcagcacca aatttcggta
     5701 gcgcttcata ccttctcctt aagcggcttg cccagaatcc ctgtgggccg taccaagagg
     5761 aatatgatca ggataccgaa gatgaaagca tcgcgatatc cagaaaggtt aggaataaaa
     5821 gccacgagta ggatttcaaa aagtccaagg gcgaaacctc cgatcatggc tccggttaca
     5881 ttgccgatcc cgcccaccac agcagcggtg aacgctcgcc agccgggagt gattcctaaa
     5941 tcccattgca atctgggata tcggcacccc caaaggatgc cccccaccgc ggccaaggcc
     6001 gatcccacaa ggaaggtgta agagataatt ttgttgatgt ccgcccccat gatctccgtt
     6061 gcctcaatgt cggtagcgat agccctcatc gcacggccaa tctttgtgtg atagatgagg
     6121 aagaggagcc caatcagaca ggctaagccg atcaggggga tccaaactgt gagggcttga
     6181 atacgcaatg tgcccaaaga gaaaatttgg ctcatgaatt ccggcctggg gaatgacttt
     6241 tgtcgtccgc ctgcccatgg aagtacaatg atcaggtttt ccaggaggaa cgatgctcca
     6301 attgcggtga tgaggaggga gattcgggga gcctccctaa gaggccggta ggcgaatcgc
     6361 tcgatcgcca tccccatccc agcggtaaga accaccgcga ggatagcccc gatccaccag
     6421 ggcaggtgaa acaaaaaaaa gctaaagaat acccaataac ctgcccacat aaagatatcg
     6481 ccatgagcaa agttaattaa cctgaggatc ccgtacacca tggtataacc gatagcaata
     6541 agggcataca ggctccccag tgtgattcca ttgaacagtt gctgtacgta gaacaccact
     6601 gtgcctccca ggaatagggg gtgagccaag tagtcaactt tggcccacct caggaaatat
     6661 tatttaccta ttagggctct gatttcccag gcttgttcta cggggatcgt ctccaccaag
     6721 gagaacttgc cttctttgac cgttagaaag gcgaagtcct tctttgggtc atttttaggg
     6781 gtcatgatta catgcccggt tatcacatcg aggtcagagg tcttgagagc ttgctttatg
     6841 gctgcaggat tagctgagcc agccctcttg atggcgtcca cgatcatcat ataacagtcg
     6901 acggtcaggg caccaaaggc atcaggatcc atgccaaatt tggcccggaa ttctttgacg
     6961 aacttcttgc tggcctcatt cggcgtaact tctgcactcc aaaacgtgag gaaattcaag
     7021 ccctctacgg catccccacc gatcttgatc aattcaggag catagagacc atcagcgccg
     7081 aggatgggtt gcatcatgcc caggtctcgt gcttggcggg ccatcatcgc tacctcagta
     7141 tagtagttag gcgtgtagat gatatcaggg tttttcctct ggatctcggt gagctgggcc
     7201 gtgtagtcga catctcccga acggcagtag agctctgcta caatcttgcc acctaaagcc
     7261 ttaaacgcat ccatgaagaa ctgcccaagg ccgacgcagt aggcttgagc catatcgata
     7321 accaccgctg ccgtcttggc cttatatctc ttctgtgcga tgctggcagc taacggacct
     7381 tggtcgttgt cggtgaagca agctcggaaa cagtaatccc ccacctgagt gcacaagggg
     7441 ttggtggtgg tagggccaat gataggtacc ccggatgcct ggcagatctc cgctgcagca
     7501 aggtaaggcc cagagcacat ggttccgatg atagccactg ccttttctgt gttgattaga
     7561 cgggtggcgc cgttggtcgc ttctgtgggc tcggtccggg catcgaaata gacaagctca
     7621 atgggccggc cgagcacctg cacggggaca atccccttcg ctcgagcgag ctccaatgca
     7681 tcccgtccca tctgaccata ggcagcacat gcaccggtga actccacaag caccccaatc
     7741 ttgattggct cttgagcgaa gactacggtt cccaagagca aattcacgat aatcaatcct
     7801 aacacaagtt ttcgcatgat ttctcctcct taaatcttat accgaaatta ccgcaaaagc
     7861 cttcgaagaa ctttgcccat ctccttctca ggcacatcac ctcctttgaa ggcccgaaaa
     7921 agataattaa ttatacatct cgccgctcaa gttagcaaga caatgtattg ctcatcaaaa
     7981 atttagctga aatgaaatta attcctcatc ataaattcta agtctggcag agtgtgacgc
     8041 cttagcaaaa aatacaaggc ggaggcacag caatgggctt acgaatgaag gataggcaat
     8101 cactcactag gaaaatcgta ccgcgctatc aaaaagcaag gaaagcagaa aaagaaaaga
     8161 ttttagatga gtttgtaaag aatactggct atagtcgcaa ctatgccatt catatccttg
     8221 cgacatgggg ggaagtacat actcttgtga tcgatgaaaa gccaatacga gtggtggtag
     8281 gagtttcttg gacacggaaa aagcgcaagg aaaatgtgca ctatacgata agtccagcaa
     8341 cgattgatcg gctcttgaaa gttgaacgaa agaaactcga aatcaaaggt aggtcgcata
     8401 caaaaggagg agtactcctt aagcatcaga ttcccatcag tacgttttat gactggtatg
     8461 agcgaaagcc tggcttcttt accagaggct cttagaaagt cctaatcttg atgagaaggc
     8521 gaagacaaat ctacgccaga gggcagcagg cctccaccaa tcgagctcaa acatctactc
     8581 gatgatgcag tcac
//
LOCUS       DAOOWY010000078         8501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_8278_length_8501_cov_19.432749, whole genome shotgun sequence.
ACCESSION   DAOOWY010000078 DAOOWY010000000
VERSION     DAOOWY010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8278_length_8501_cov_19.432749"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PK105_06550"
     CDS             <1..709
                     /locus_tag="PK105_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOU61408.1"
                     /translation="KTSVPPNLSDSSILAFSLSKADHVGIYLGDDIFIHAASTGAKTG
                     VIRSSLKERSWQQRFLFAGRALPASGFSGFALDWGSGISFAQLDMLLQGDLLAFVRGI
                     SGWTEVSLPVANNLSAGLRAGVQWDRVLGVVRVPIELDLGRISGVSVFAGPAITFGLP
                     TLNGRLYTQGDFFLATGGLRWSSFFYSRGAHRLGSYIELRYDYYVPKPEQVESIRSDL
                     QSCLNISIGFRLRNVFY"
     gene            complement(703..1530)
                     /locus_tag="PK105_06555"
     CDS             complement(703..1530)
                     /locus_tag="PK105_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOU61409.1"
                     /translation="MLTTILLTIGFSAALAFILGVALGFFNEKFKIELDPKISEVRQA
                     LPGANCGACGYPGCDGYSEAVATGRAPTTRCSVGGSATAEVLSKIMGIEAGTVEEKVA
                     VLLCQGAIDKTVNKGEYSGIPTCRAAKLSTGGIKACSWGCQGFGDCTLVCKFDAIEMG
                     EDGLPHIDYDNCTGCGLCVEECPQKILTLIPLTKVGSVVLCSNRTVLKQTVLKNCKVG
                     CIKCDLCVRSCPEGAISMVNGIPVTDYAKCNSCGICVQKCPTKCYKLLEKDVLVVHQ"
     gene            complement(1543..2118)
                     /locus_tag="PK105_06560"
     CDS             complement(1543..2118)
                     /locus_tag="PK105_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOU61410.1"
                     /translation="MDLGKILLLSMLVNNIVVMRFIALCSYIGMTSDLGQSMGMGYAV
                     TFVTVLATAATWPIYHYILLPLDLAFLQILVFILVIASLVQLVEFYLKKNIPGLYAAM
                     GIYLPLITTNCAICAVTFENITNNYNFIQSIVYAIGVSLGYLLAMILLAGIRDRMKTS
                     PVPKFLQGTPILFMATSLIGIAFMGFAGLIK"
     gene            complement(2118..2759)
                     /gene="rsxE"
                     /locus_tag="PK105_06565"
     CDS             complement(2118..2759)
                     /gene="rsxE"
                     /locus_tag="PK105_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOU61411.1"
                     /translation="MKKLYSIFKQGIIFDNPLLMLMIGLCSAIAVTTNVSNGIGMGLA
                     MTFVLLFSEIIISLFRKLIPSMIRIPIFIIVIAAFTTIVDLVMKAYFPDLSKSMGVFI
                     PLIVVNCIIMGRVEAFASKQDLLSVIADALGMGLGYTWVLVGLAAIRELLGNGTFAGI
                     QIMPASYEPILFFILPPGGFFVFSLFISLNIWLKKKMEAAARRKELVSKKEAA"
     gene            complement(2762..3481)
                     /locus_tag="PK105_06570"
     CDS             complement(2762..3481)
                     /locus_tag="PK105_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOU61412.1"
                     /translation="MKKTKLGVILMLYASIACASLAIVYAFTKDSIERQSEIQLQTSL
                     KEIFPQASTFELVEDLTSPEPNIKFEAVYEVKSDAVPLGLAIKASGPSYGGNATLLVG
                     IDLHRSIAGVRIVALNDTPGLGMNASNPTYYVDKVKKVTFLGQFEGKYIADPFEVKKD
                     VEAITAATITSKSLAKIIKTAGDAAIAYMDQKVMMASMEVEKPNGETSIVAPPTASTG
                     FPIGSSEGKSKEKIPPQAGGK"
     gene            complement(3478..4488)
                     /locus_tag="PK105_06575"
     CDS             complement(3478..4488)
                     /locus_tag="PK105_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOU61413.1"
                     /translation="MSDKKIILSSSPHTFSQVDTPRIMISVVTALVPAAAYGVYLYGL
                     AALGVIVTSITSAVLSEFLFRKLIRASTTIGDFSAVVSGFLLALVLPPGTPLWMVALG
                     AAFAIVVAKEFFGGLGANPLNPALIGRAFLLMSFPAAITSWNMPKGLALPADAVSAAT
                     PLNLIKQGNSLADVAKYFGASDIGAFYRQLFMGYRPGSIGESSILLVLLGGLFLLAIG
                     VIQWIVPVSVLASTFVFSWLLGMDPLLSLLTGGIAFGAFFMATDYSTRPMTEKGQAIY
                     GIGIGLIAVLIRKFGGYPEGVTYAILIMNILTPFLNKLRTKKYGFAHPSKPAKPSKEA
                     RK"
     gene            complement(4478..5839)
                     /gene="rsxC"
                     /locus_tag="PK105_06580"
     CDS             complement(4478..5839)
                     /gene="rsxC"
                     /locus_tag="PK105_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOU61414.1"
                     /translation="MSMRTFRGGAHPSERKDRSQHAPIEKVNSVPQVVIPVNQHFGAP
                     IQPLVKVGDFVRVGQKIADAEGRMTVPLHASIAGVVKKIEPRMQSNNADGLCIIIEPK
                     DSPAGESDREFMPPLDPFACSKEEALSRIREAGIIGMGGAGFPAHVKLAPTKPISVVI
                     ANAAECEPYLTIDERTLLEMPDKIIDGLSIVMKIINAAEGVVAIEENKKHTLPLVEKA
                     IQENPQVKAGMKIGIQVLKTKYPQGGEKMLITAITGRQVPSGGLPMDVGCVVSNVGTL
                     CAIADAFREGKPLIERGFTVTGGACRTPKNLVVPVGTVVADLAKTGIIDIDEESLSKV
                     IFGGPMMGVAVPSSEIPVQKNTSGVVLMSVEEALQFEEKTCIRCGRCIRACPMRISPA
                     LLAIAIESGNYSEAEKIGLQDCIECGACSFVCPARRYLVQRFRIGKQVLRALKQKEAQ
                     NVR"
     gene            complement(6155..6877)
                     /gene="jag"
                     /locus_tag="PK105_06585"
     CDS             complement(6155..6877)
                     /gene="jag"
                     /locus_tag="PK105_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOU61415.1"
                     /translation="MIYEFEGKTEREAMELATQELGLDTSSFDVEIIENQSGGLFKKG
                     KVRIRVHTKEATSEDNREKESNEHNKFGEREEARDERHIEPLPMEDFENKIIEWTSEV
                     ISLMGYSAQVSVAFREPNKLGLRIDTEAASILIGKKGRNIDSLQLLANVYAGNLGHSD
                     TKIVLDSENYRLRREEALVRVAYKTAEEVRSTGHSILLEPMNPFERRIIHTTLNDIVD
                     IETKSEGDGLMKQVRVMLKGRN"
     gene            complement(6910..>8501)
                     /gene="yidC"
                     /locus_tag="PK105_06590"
     CDS             complement(6910..>8501)
                     /gene="yidC"
                     /locus_tag="PK105_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOU61416.1"
                     /translation="TIAFSRMFEAKMAGSDEKVPFIFKKVFSFRDGEYLFGLAISIEQ
                     PEGKPIFLGTNGVAYRIDLGPQIGPRFDQMPKNADYRKYIAEIDGKKKKQNLKANTSD
                     TIAPNASWVALSGKYFTFIAIPQAPISAYEIATAQDPLINQTNSLGLLRSTFSGSSTT
                     DTYYFYFGPKTSAELGKYEYADKNGFGLSNLNLEDAMEGSGILGWLETILKFLLNVFY
                     RLIPNYGIAIILVTVLIKLLFYPLTKKGSLATARMSELQPKMQELQAKYKNNPQKLNQ
                     EMAELYKREHYNPMSGCLPMLIQFPLFIAMYNLFNNHFELRGAMFIPGWINDLSLPES
                     LVNFGNFRIPLLGWNDLRALPIIYLFSQLMYGKFTQAPQSTQGNNPQANQMKLMMYGM
                     PIMFFFILYDVPAGLLIYWITNNILTIAQQILINNLMKKRKMAGAAAQAAEGTPAAGA
                     ANAGPRLATGKSSAVDTKAKTVGRVPAKEGFSERVTNWLEKQAGEAEKASGSAYTKKK
                     DQGRTSGSDRSSAPRKNKAKK"
BASE COUNT         2193 a         2088 c         1869 g         2351 t
ORIGIN      
        1 gaaaacctct gtccctccga acttgagcga ttcgagtata ttggcatttt ctctttcgaa
       61 agccgaccat gtgggaattt atctgggaga tgacattttt atccatgctg cgagcactgg
      121 agctaagaca ggagttattc gcagttcact gaaggaaaga agctggcaac agcgatttct
      181 gtttgcgggg cgcgcgcttc ccgcttctgg tttttctggc tttgccttag actgggggag
      241 cggaatcagc tttgctcaac tggatatgct tctacagggc gatcttttgg cctttgtgcg
      301 cggaatctct gggtggacgg aggtttcgct tcccgttgca aacaacctta gcgccggtct
      361 aagggcaggc gttcaatggg atcgtgttct tggtgtggta agagttccta ttgagcttga
      421 ccttggccgg atttctgggg tctcggtgtt tgccggtcca gcaattacat ttggattgcc
      481 aacgctgaat ggcaggctgt acacccaggg agatttcttt ttggctacag gagggttgag
      541 atggtcgtct ttcttctatt caaggggtgc tcatcgactt ggtagctaca ttgaactcag
      601 atacgattat tatgttccta aaccggaaca ggtcgaatca attagatccg atcttcaatc
      661 ttgcctcaat atctcgatag gttttcgctt gcgcaatgta ttttattgat ggactactag
      721 cacatctttc tcaagcaact tgtagcactt tgtcggacac ttctggacac atatgccgca
      781 agagttgcac ttggcataat ctgtcacggg aatgccattc accatcgaaa ttgcgccttc
      841 cggacaagaa cgaacacata gatcgcactt gatgcatccg accttgcagt tcttgagtac
      901 ggtctgcttg agcacagtcc tattcgagca gagcaccacg gaaccgacct ttgtaagcgg
      961 tatgagcgtc aatattttct gtggacactc ctctacacat aggccgcagc cggtgcaatt
     1021 gtcataatcg atatgaggaa gcccatcttc tcccatctcg atggcatcga acttacagac
     1081 gagcgtacag tcaccaaatc cctggcatcc ccagctacag gccttaatac cgcccgtgga
     1141 tagcttggca gccctacagg tgggaatacc gctgtattcg cccttgttta ctgttttatc
     1201 gatcgcgcct tggcacaata gaacagctac cttctcttcg accgttcctg cctcgatgcc
     1261 cataattttc gaaagcactt cagcagtggc tgatccaccg acagaacacc tggttgtggg
     1321 ggcgcgacca gttgccaccg cctcggaata accatcacaa cctggatagc cacatgcccc
     1381 gcagtttgcc ccgggcaaag cctgtcttac ctcgctgatc tttggatcta gttcgatctt
     1441 gaatttttca ttaaagaagc ctaatgccac tccaaggata aaggcgagcg ctgcagaaaa
     1501 tcctattgtc aataaaattg tggtaagcat ctcgatatcc tcttatttta tgagccctgc
     1561 gaaacccata aacgctattc caatcaagga cgttgccata aaaagtatcg gcgttccttg
     1621 taaaaacttc ggtacagggc tcgtcttcat tcgatccctt atgccagcaa ggagaatcat
     1681 cgccaagaga taaccgagcg agacgccaat tgcgtacaca attgattgga taaagttgta
     1741 attattcgtg atattctcga aagtaaccgc gcaaatagca caatttgttg ttataagtgg
     1801 caaatagata cccattgctg cgtacaaacc gggaatgttc ttcttgagat agaattcgac
     1861 gagctgaacc aggcttgcaa tgacaagaat gaataccaga atctgaagaa aagccaaatc
     1921 aagcggcaga agaatatagt ggtaaatagg ccatgtcgct gctgtagcga gcactgttac
     1981 aaaggttact gcataaccca ttcccatgga ttgccccaga tcgcttgtca ttcctatata
     2041 tgaacagagt gcaataaagc gcattaccac gatattgttt accaacatcg acaaaaggag
     2101 aattttgcca agatccatta tgcagcctcc ttcttagaaa ccaattcttt tcttctcgca
     2161 gctgcctcca tcttcttctt gagccatata tttaaggaaa taaacagact gaaaacaaag
     2221 aaacctcctg gtggcaaaat aaagaagaga atcggttcat aggaggcagg cattatctga
     2281 ataccggcaa aagtcccatt cccaagcagc tctcttattg cagcaagacc aacaagaacc
     2341 caggtataac cgaggcccat tcccagcgcg tctgcaatca ccgataataa atcttgctta
     2401 gatgcaaagg cttcgacacg ccccattatt atgcagttga cgacgatcaa tgggatgaaa
     2461 acacccatgc tcttcgaaag atcgggaaag taggctttca tcactaagtc aacgatcgtt
     2521 gtaaatgctg caatgactat gataaaaatc gggattcgaa tcatagatgg aatcagcttg
     2581 cgaaatagag agattattat ctccgaaaac agaagcacaa aagtcatggc caagcccatg
     2641 ccaataccgt tcgagacatt agtcgtcacc gcaatggcag aacatagacc gatcatcagc
     2701 atcaatagag gattatcaaa aatgatccct tgtttgaaaa tagaatacag cttcttcatt
     2761 ctcacttgcc tcctgcttgg ggcgggattt tctctttaga ttttccttcg gatgagccta
     2821 tgggaaatcc tgtggaagcc gtaggaggtg ccactataga tgtttcgcca tttggctttt
     2881 cgacttccat cgaggccatc atcaccttct gatccatata ggctattgcg gcatcgccag
     2941 cggtcttgat gatcttggca agagacttcg atgttattgt ggccgctgta atcgcctcaa
     3001 catccttctt cacctcgaag ggatcagcaa tatattttcc ttcaaattgg cctaagaacg
     3061 tcaccttttt gactttatcc acataataag tcggattaga tgcattcatt ccaaggcccg
     3121 gcgtatcatt gagcgctaca atccttactc ctgctatgct gcgatgcaaa tcaattccaa
     3181 caagcagggt tgcattgcct ccgtagcttg gtcccgaggc ttttatagcc aatcccagtg
     3241 gaaccgcatc gctctttacc tcatatactg cttcaaattt gatattcggc tctggacttg
     3301 taagatcttc gaccagctcg aaggtacttg cttgtggaaa gatctcctta agggaggtct
     3361 gcagttgaat ttcgctttgt ctttcgatgc tatccttggt aaaggcgtag acaatggcaa
     3421 gacttgcaca ggcaatcgag gcatatagca taaggatgac gccaagtttt gtctttttca
     3481 ttttctagcc tccttggaag gcttagcagg ctttgaagga tgcgcaaagc catatttttt
     3541 agttcgcagc ttatttaaaa atggcgtcaa aatattcata atcaatatcg cataggtcac
     3601 tccctcagga tatccgccga attttcttat aagaacagcg atcaggccaa taccaatacc
     3661 atatatggcc tgacccttct ccgtcattgg ccttgtagaa taatccgtcg ccataaagaa
     3721 tgcgccaaat gctatgccac ctgtcaacaa gctcagcaat ggatccattc ctaaaagcca
     3781 agagaacaca aatgtggatg ctaacacaga taccggcact atccactgaa tgactccgat
     3841 ggcaagaaga aaaagcccgc ccaagagcac aagcaaaatc gagctctctc cgatggagcc
     3901 ggggcggtat cccataaaca gttggcgata gaatgccccg atatcgctcg ctccgaaata
     3961 cttggctaca tcagctaatg aattgccctg cttgattaag ttcaatggtg tagccgcact
     4021 gactgcgtct gcgggcaatg caaggccttt cggcatgttc cagctagtaa tcgccgcagg
     4081 gaagctcatg agtagaaaag cgcggccaat caatgcggga tttaagggat ttgcgcccaa
     4141 tccaccaaaa aactcttttg caaccacaat agcaaaggca gcacccagcg caaccatcca
     4201 gagcggagta cctggaggca gaaccagcgc aagtaaaaac ccgcttacaa cagccgaaaa
     4261 atcgccaatc gtggtgctgg ctcttattag ctttcgaaat aggaattcgg acaacacggc
     4321 cgatgtaatc gaagtcacaa tgactccaag cgcagccagg ccatagagat aaacaccata
     4381 ggccgcggct ggcaccaacg ctgttacgac ggagatcata atgcgcggag tatcgacctg
     4441 cgagaaggta tgggggctcg aagataggat aattttctta tctgacattc tgtgcctcct
     4501 tctgtttcag cgcacgaagt acctgtttcc caatcctaaa gcgctggaca agataacgac
     4561 gagctgggca cacaaatgag caggcgccac attcgatgca atcttgaagg ccaatctttt
     4621 cagcttcgct ataattgccg ctttcgatcg caatagcaag taaagcagga ctaatcctca
     4681 tagggcatgc tctaatacaa cgaccacaac gaatacaggt tttttcctcg aactgcaatg
     4741 cttcttcgac actcatgaga accacgcccg aagtgttctt ttgtaccggt atttcgctgc
     4801 tcggtacagc cacacccatc attggtccgc caaagatcac tttggaaagc gattcctcat
     4861 cgatatcgat tattccggtt ttagcaaggt ctgccaccac ggttccaacg ggtactacaa
     4921 ggttctttgg ggtcctgcac gctcctccgg tcacggtaaa tcctcgctct ataagtggct
     4981 tgccttccct aaacgcatct gcaattgcac acaatgtccc aacattagat accacacagc
     5041 cgacatccat agggagacct ccggacggta cctgtctgcc tgtgattgct gtgataagca
     5101 ttttttcgcc gccctgtgga tattttgtct taaggacttg tataccgatt ttcattccgg
     5161 ccttgacctg cggattctct tggatggctt tctctaccag tggaagcgta tgtttcttat
     5221 tttcttctat tgctacgaca ccttcggctg catttatgat tttcatcact atcgaaagac
     5281 catcgatgat tttatccggc atctccaaga gagtacgttc gtcaattgtc aaataaggct
     5341 cacattccgc tgcatttgca atgaccacag agattggctt tgtaggagcg agctttacat
     5401 gagccggaaa gcctgcgcct cccataccaa tgattcctgc ttcgcgaatg cgcgagagcg
     5461 cctcttcctt actgcatgcg aagggatcga gcggaggcat gaattcccta tcggattctc
     5521 ctgctggtga atcctttggc tcaattatga tacatagacc atctgcattg ttagattgca
     5581 ttcgcggctc gattttcttt accacgcctg ctatcgacgc atggagaggt acggtcatgc
     5641 gaccttccgc atctgctatc ttctgcccaa ccctcacaaa atctcccacc ttgactagtg
     5701 gttgaattgg ggcaccaaaa tgctgattga caggaatcac aacctgcggc accgaattaa
     5761 ccttctcgat tggcgcatgc tgcgagcgat cctttctctc ggacggatgt gccccgcccc
     5821 tgaatgtcct cattgacata gtggaaaggt ctcctttgaa gatttgcttt gacctcgcga
     5881 agaatccaat cgtcttcgca ttcaatgtaa aatgatagaa aattataaca aaatcgagct
     5941 atattagcac cagttttcaa ttgtggtcaa ggcaaaaaga gcataagtat tcgctataca
     6001 ataaaaagat aaaaaattat ttaatattgg tccgaaaaat tgagatgaaa catgacaacg
     6061 aaatatctcg gcaaaaaaga tagcgccgcc atgctgtgct gcggcgcctt attcgtatca
     6121 gccagattgt tatgccagat ccacggaatg cttactaatt tcttcccttt agcattacgc
     6181 gtacctgctt cataaggcca tctccttcgc tcttcgtctc tatatcgacg atatcattga
     6241 gggttgtatg gataattcga cgctcgaagg gattcatagg ctcaagcaag atggaatgcc
     6301 cagtactgcg cacttcctct gcggttttgt aggcgactct gactaaggcc tcttcacgcc
     6361 tcaagcggta attctcggaa tcgagcacga tcttggtgtc gctatgccca agattgccag
     6421 catagacgtt tgctaaaagc tgcagcgaat cgatgttcct gccttttttt cctatcagaa
     6481 tcgaggcagc ctcggtatct atgcgcaagc caagtttgtt aggttcccta aatgccacgg
     6541 agacttgagc actatatccc atcaagctaa ttacctcact cgtccactca ataatcttgt
     6601 tctcgaaatc ctccattgga aggggctcta tatgcctctc atcccgggct tcttctcttt
     6661 ccccgaattt attgtgctca ttggattctt tttctcgatt atcctcagag gttgcttctt
     6721 tggtatgaac ccgtatgcgc acctttcctt ttttgaataa gccaccactt tggttttcga
     6781 tgatctccac atcgaatgaa cttgtatcca ggccgagctc ctgagtggct agttccatag
     6841 cctcgcgctc ggtctttcct tcaaactcat atatcatttg gatcgctcct gattgaaaga
     6901 ttcaatggtt tattttttgg ccttgttttt tcttggtgcc gaggatctgt cggaacctga
     6961 ggtgcggcct tgatctttct tctttgtata agcagaaccg cttgcttttt ctgcctcgcc
     7021 agcctgcttt tccagccaat tcgtcactct ctcagaaaaa ccctcttttg ccggcactct
     7081 accaacggtc ttcgcttttg tatctaccgc cgagctcttt cctgtcgcta atcttggacc
     7141 agcattcgct gctccagcgg ctggcgttcc ttcggccgcc tgtgctgctg caccagccat
     7201 cttgcgtttt ttcatcaaat tattgattag gatctgttga gcaatggtca gaatattgtt
     7261 tgtaatccaa taaatcagaa ggccagcggg cacatcgtag agaatgaaaa agaacattat
     7321 aggcatgcca tacatcatca acttcatctg attagcctgc ggattgttgc cttgggtgct
     7381 ttgaggggct tgggtaaact tgccatacat taattggcta aataggtata tgatgggcag
     7441 tgcccttaaa tcgttccatc caagaagagg aattcggaaa ttgccaaagt ttacaaggct
     7501 ctcaggcagg gacagatcat tgatccagcc tggtatgaac attgctcccc tgagctcgaa
     7561 gtgattgttg aaaagattgt acattgcaat gaataagggg aattgaatga gcattggcag
     7621 acatccagac attggattgt agtgttccct cttatatagc tcggccattt cctgattgag
     7681 cttctgtgga ttgttcttat acttggcctg caattcttgc atcttgggct ggagttccga
     7741 cattctcgcc gtagcgagcg accctttctt tgtaagtggg tagaatagca gctttatcag
     7801 cacggtaacg agaatgatgg ctataccata attaggaatt aggcgataaa aaacattgag
     7861 caggaatttc aatatggttt ccaaccatcc aagaatgccc gagccttcca tggcgtcttc
     7921 caaatttagg ttggaaagac caaatccgtt tttgtccgca tactcatatt ttcccaactc
     7981 cgcggatgtc ttgggtccaa aatagaaata gtaagtatcg gtagttgagg aaccagagaa
     8041 ggtgcttctc aaaagcccca gactattggt ctggttaata agcggatcct gggcggttgc
     8101 tatttcgtaa gccgatattg gtgcttgtgg aatagcgata aatgtaaaat attttccgga
     8161 caaggctacc cagcttgcgt ttggcgctat ggtgtctgag gtattggcct tgagattctg
     8221 tttctttttc ttgccatcga tttctgcgat gtattttcga taatcggcat tcttgggcat
     8281 ctgatcgaag cgcggaccaa tctgtggtcc aaggtcgatc ctataagcaa ctccatttgt
     8341 tcccagaaaa atgggtttgc cttcgggctg ttcgatgcta attgcgagcc caaatagata
     8401 ttctccatcg cggaatgaaa agaccttctt gaatataaag gggacctttt cgtcgcttcc
     8461 tgccatcttt gcctcgaaca tcctcgagaa tgcaattgtg t
//
LOCUS       DAOOWY010000079         8451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_8339_length_8451_cov_47.809790, whole genome shotgun sequence.
ACCESSION   DAOOWY010000079 DAOOWY010000000
VERSION     DAOOWY010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8339_length_8451_cov_47.809790"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PK105_06595"
     CDS             <1..836
                     /locus_tag="PK105_06595"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOU61417.1"
                     /translation="IQGEVKRPGTYQLLPGEGLKELIDLYADGFTEKANTSRLTLLHY
                     LSDSSPVGEKLQFDYAKFPDIKLRLYDVITVPSLQELLPVVWFEGAVGVSSTGSSPET
                     SQRVAYNFVPGETVSQAALNNRKLFSAVSDLASGYIIHADGSTTAIDLAKFIYDYDLT
                     NDLPLKANDTIIVPFRQFFVSVSGAVRYPGRYPYIPKRTWEYYIGLAGGFDTDKNSGQ
                     KITIYDVKSQKVAIAGREIQPEDNIVAESNSFTYNMMKVSSIISTVLSFTVLIISLLG
                     L"
     gene            919..1548
                     /locus_tag="PK105_06600"
     CDS             919..1548
                     /locus_tag="PK105_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOU61418.1"
                     /translation="MNYSNPELQLLQSIYKVTDEGNSPALVSQRQLANSAGLSVGLTN
                     ALLKRFIERGWVKLLHIDGRKIKYALTPSGMEEIALRAVEYFTRAERNTELYRKKIDF
                     FIEGIAHEGYEALLLVGLSELDFLFDYACLKHGIGFYKSVKLFMREIDENDLSWAELV
                     VVCADNFEDSEEIKGDSTDIKQYIGLPEEVPLIKFSQLLMNAVVEKGKS"
     gene            1630..2721
                     /locus_tag="PK105_06605"
     CDS             1630..2721
                     /locus_tag="PK105_06605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOU61419.1"
                     /translation="MSYEVALSSAFLSALTMPIILNFAHKNSLYDSLDARKNHNGNIP
                     RLGGVGIFVAFATMMFGLILIQPGTVAKELASRASRLWPLALSGMIMHIIGLLDDIKA
                     QSPRLKLAIQIVAAIMVVASGYRFRGFGFRQDAMQGQLAWFSVILSVGWIVGVTNAVN
                     FIDGIDGLAGSVSFIVAMVYGTLYYNFGDASSSLICLSIAGVIVGFLFFNFPMPNAKI
                     FMGDSGSLFLGFSLAVMPFLGQVSRGESLKLNIGLFPSITILALPVFDALRVMGLRIA
                     EGRNPMSPDRQHVHHLFFDSGYSKLRIDLIFSLVTAGLAFVVLIAADMPQSLGFMFEF
                     IAMGLLMIFFRYVKYVNKESIGNNGRKAG"
     gene            3061..5241
                     /locus_tag="PK105_06610"
     CDS             3061..5241
                     /locus_tag="PK105_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU61420.1"
                     /translation="MKKAICWLLALSIAFIFPAMLTAQEKRVALVLGNGDYQATTKLI
                     NPVNDAADIGEMLAGLGFDVEVRIDADLDTMEKAVLRFGNKLAGSTDSVGFFYYAGHG
                     VQSNGENYLIPVDARLSAESMLRTRAIPLQFVLDSIGEAGNKLNIIVLDACRDNPFSW
                     ARSSARGLAVVGIQPAASIVVYSTSAGKVAQDGTGRNGTFTEELLRHLPTPGLDIMEV
                     LRRTGQDVQAKTAGVQIPAIYSQFFGFMQLVPGETPVSPGPAIGIETGVKAGPAAPIE
                     TSAKAGSSPGSETSAGSSSGSSSGLSPESGGVLDYLPAFFDQAPANSKLTIAKAEELT
                     WDGQWLSAWRILEKADPNNRDPYVLTEKIRTMLDGNTSLDLFQGFSIADMPPDGDWEA
                     ACEAGTLDEEYFDFDPHSAVQALLKRGVQVPPVLATALGDFYYSAYYDYVDNSSLSLD
                     EIRDNALYWYDIANEDFILVELQSILHYAELLMDAGRADEAVAILEDEKEWEPEDTEL
                     RLQLIDAYIATENNSSAFLELDGLIERAASKEDAREFYRRAFDLALEIQDQQALNRYL
                     AAFEKKYPNDWLAGLVRHKVALQMGNYTAAQTIADGLMKQFPLKTELLEGLLSNWLDY
                     LDYNSAIAEAGLAFLDRWVVASQWNPVALGTYCLYRSLYRYMIIQNWPPSYMKNVAIQ
                     NAFTDLNIAEMNLSAARNALSEEGKALLDMIPDFRNELQSAIGY"
     gene            complement(5352..6374)
                     /locus_tag="PK105_06615"
     CDS             complement(5352..6374)
                     /locus_tag="PK105_06615"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU61421.1"
                     /translation="MQPELESMPSKPPLNLIRGILIAYAVGDAMGMPTEFMTRAEISS
                     RFGLVDRLLEPSQSKNHPNLPKASITDDTEQVCTLLEEYSEKDSIDPYITAKRLLRWM
                     RDSNAIEKGYIGPSSRLALEAIERGESPEEAGKKGTTCGGVMRSPAAVLFAAARQRPL
                     ADCVRACLIPTHNTQLAMEPAMAYGYALLAAIYGKNIEEILSEAKKGAEAARSAPDSQ
                     GYPQCGASIMARLLHFKKLASSFSDTDEILDFIYYIYGTTLTSVDVAAAAMCIFLASM
                     DDVWLSIRMGASVGGDTDTIAALAGALSAAYALAQGQSLNIPEAIVSEVLENNKLDFN
                     RLAERL"
     gene            complement(6388..7275)
                     /locus_tag="PK105_06620"
     CDS             complement(6388..7275)
                     /locus_tag="PK105_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU61422.1"
                     /translation="MAETAKTSDAKAADEMTPDEKAFNAKAANKYSFSTAALYPRSAA
                     ESLRLIAGAGFLHAELMPQCFADASESFALKAERSKIHVASVHYPLAMFSMLYNASPG
                     MISEERAFAEGLVRLCAALGAEVLVIHPHDIQTEVQLKSSIEQPIRENILHLAEQCDK
                     AKIVLAVENSPKGAGRTAEGLLEYIESLGNSSIKPMVDTTEACEAGLDPAEFILKVSP
                     VHLHLSDHSGEKKHLPAGEGDTDWQAIKNALKIKAYAGYYTLEPVYRYYLAEPEEKLA
                     LAYDFVSGLFGSTSVTSGG"
     gene            complement(7268..>8451)
                     /locus_tag="PK105_06625"
     CDS             complement(7268..>8451)
                     /locus_tag="PK105_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61423.1"
                     /translation="GAQTKLKVWFAVSGDSGEAFKALLEKYKAANPNVSIEYSYSGNY
                     ADTATKVSAALLSNTAPDVALMAAGPLYTGARDDFFIESKLNDPDFNKDDIYPGVWDY
                     AKYNGRICAVPYGISTQVMYYNKAIIEKAGINLKNPPKTWNEFLQVAKTAQTKGNITG
                     TPDFWGFDVTDVPWLFKTMLNQNENSVISVKGTKISPAFNDDKGVEVANFWKRFVDEK
                     VMPVGQHSNAEKKFLAGNLAFLAATSARISRWSKDPNLKIGAIPMPYFKRPSVALGGN
                     VLVILSQDPKIREAAWNLVKFLASKENQTSFALNTGYLPIRKSGLTLPEAKAAVTENP
                     MYAIAFDQLDISWSYWHFEQMGTMDQLLAQAVDALERGALTPKAALDKAAKELQAEID
                     G"
BASE COUNT         2071 a         1904 c         2167 g         2309 t
ORIGIN      
        1 caatccaggg tgaagtaaaa cgccccggaa cctaccagct tctgcctggt gaggggctta
       61 aggaactaat cgatttatat gcagatggtt ttactgaaaa agccaataca tctcgcctta
      121 ccttattaca ttatctatcc gatagctcgc cagtgggaga aaaattgcag ttcgattatg
      181 ccaaatttcc ggatataaaa ctcaggcttt atgatgtgat aacagttcct tctttgcaag
      241 agcttttgcc tgttgtatgg ttcgaaggcg cagttggagt gagttctacg ggctcatctc
      301 ccgagacgag ccagcgcgta gcatataatt ttgtacctgg tgagacagta tcgcaggctg
      361 cgcttaataa tcgaaagctc ttttcagcgg tgtcggatct tgcatctgga tatatcatac
      421 atgccgatgg ttcgactaca gcgattgatc tagcgaaatt tatctatgac tatgacctta
      481 ctaatgacct tccactgaag gcaaatgaca caataatcgt tcctttccgt cagttctttg
      541 tctctgtatc gggggcggtg aggtaccctg gtcgctatcc atatattccg aaacggacct
      601 gggagtatta cataggactt gctggcggtt tcgatacaga caagaattcg ggtcaaaaga
      661 ttactatcta cgatgtcaaa tcgcaaaaag ttgcgattgc aggccgagag attcagcctg
      721 aggataatat agtcgcagaa agcaattcat ttacttacaa tatgatgaag gtttcttcga
      781 ttatctcgac cgttctttcg tttacagtgc ttataattag cctattgggc ttgtgaagct
      841 tggctgtttc atattcctat taatctaggc ctaattaatg aatcgtctcg tattcttgtc
      901 tcataaatag ttacatagat gaattattcc aatcccgagc tccaactcct ccaatcgatc
      961 tacaaggtga ccgatgaggg caatagtcct gcgctcgtgt ctcagcggca gcttgcgaat
     1021 agtgcagggc tctcggtggg acttacaaac gcgcttctca agcgcttcat cgagcgaggc
     1081 tgggtcaaac tccttcatat cgatgggcga aagataaaat atgcgcttac tccttccggc
     1141 atggaggaga tagcactgag agcggtggag tattttacaa gggcagagcg caatacagag
     1201 ctctatcgaa aaaagataga cttcttcata gaaggaattg cacacgaggg atacgaagcg
     1261 ctgcttctcg tagggctttc ggagctcgat ttcttgttcg actatgcttg cctaaagcat
     1321 ggaataggat tctacaaaag tgtcaaactg ttcatgcgcg aaatagatga aaacgatttg
     1381 agctgggcgg agctggtggt ggtctgtgcc gataatttcg aggatagcga agagatcaaa
     1441 ggcgattcaa cggacataaa acagtatatc ggtctacccg aagaagtgcc gctcataaaa
     1501 ttttcgcagc ttctcatgaa tgcggtagtg gaaaagggca aatcttgaat tgaatcgata
     1561 aaggggtctt gcaaagcagc tacgaaatag tcaataattg actatatcta aaaagaaggg
     1621 attaatgcta tgtcatatga ggtagctctc agttccgcat ttctttcggc attgacgatg
     1681 cctatcatat tgaactttgc gcataagaat agcctctacg attctctaga tgcaagaaag
     1741 aaccataatg gcaacattcc gcgcctggga ggcgttggaa tattcgttgc attcgctact
     1801 atgatgtttg ggctcatctt gatccagccg gggacggttg caaaagaact tgcttcccgt
     1861 gcaagtcggc tttggccgct tgctctgagc ggaatgatta tgcacatcat tgggctattg
     1921 gacgatatca aggcacagtc tcccagacta aagctcgcca tacagatagt agccgcaatc
     1981 atggttgttg ccagtggtta taggttcaga ggcttcgggt ttcgacagga tgcgatgcaa
     2041 ggccagttag cctggttttc cgtgattctg agcgtgggat ggattgtagg ggttacaaac
     2101 gctgtaaatt tcatcgatgg gatagatggg ctggccggca gcgtatcttt tatagtcgcg
     2161 atggtatatg gaactctcta ctataatttt ggagatgcat cgagctcgct tatctgtcta
     2221 agcattgcgg gcgtcattgt aggatttctc tttttcaatt ttccgatgcc aaatgccaag
     2281 atatttatgg gggattcggg aagtctcttt cttgggtttt ctcttgcggt tatgcctttc
     2341 ctagggcaag tgtctagagg ggagtccctg aaattgaaca ttggtctctt tccttcgata
     2401 accattctgg cgcttccagt gttcgatgcc ttgagagtta tgggcctaag gatagcggaa
     2461 ggtaggaacc caatgtcgcc cgataggcag catgtgcatc atctcttttt cgattctggc
     2521 tatagcaaat tgagaattga cttgattttc tctttggtga cggcagggct ggcttttgtg
     2581 gtgctgatcg ctgccgatat gcctcaaagt ctagggttta tgttcgaatt tatcgcgatg
     2641 ggacttctta tgatattttt taggtatgtg aaatatgtga ataaagagag cattgggaat
     2701 aatgggcgca aagcagggta gttatttcca gattcttgct attgtataag gcgtttctat
     2761 attgcttatt tgaacaggta actcaaattc gaataggtaa gcttaatcgg agcttttaga
     2821 aaaagctaaa gaaagattga gaaaaggccg ctgggattca ggttattgct tggatttcag
     2881 tggccttttt tattttccta ggggtgctct tgcggctata ttgctaattt aaaaattagt
     2941 gcacagatat gaaaaatgca ttataatatt ataagatagg ctgaaaaagc tcagaaggac
     3001 aaaaagatta agaagattaa gaaggccaga aagataaaga agattaggaa gattaagaat
     3061 atgaaaaagg caatttgctg gcttcttgct ttatctatcg cgtttatctt tcctgcaatg
     3121 cttacagcgc aggagaaacg cgtcgctctc gtactaggaa atggagatta tcaagctact
     3181 acaaagctca tcaatccggt aaacgatgct gcggacatag gagaaatgct cgccggtctt
     3241 ggcttcgatg tagaagttag aatcgacgcc gatctcgata cgatggaaaa agccgtgtta
     3301 cgctttggca acaagcttgc tggatccact gattccgttg gattcttcta ttatgccggc
     3361 catggtgtgc agtcgaacgg ggaaaattac ttgattccgg tggatgctcg ccttagcgcg
     3421 gaatcgatgc tccggacaag ggcaattcct ctgcaattcg ttctagatag cataggagag
     3481 gctgggaata agttaaatat tatagtgctc gacgcatgca gggataatcc attttcgtgg
     3541 gcgcgatcga gcgctcgagg gcttgcggtg gttggaatcc agcctgcagc aagcatcgtt
     3601 gtgtattcga caagcgctgg gaaggttgcc caggatggaa ctggtcgcaa tggtacgttt
     3661 acagaagagc ttttacggca tttgccaacc cctgggcttg atataatgga agtattgcgg
     3721 cgcacaggcc aggatgttca ggcaaaaacc gctggcgtac agattcccgc gatctatagc
     3781 caattctttg gatttatgca gctggtaccc ggagaaactc cggtaagccc aggtcccgca
     3841 ataggcatag aaacaggtgt taaggcaggc cctgcggctc caatagaaac aagcgccaaa
     3901 gcaggctctt cgccgggctc cgagactagc gctggttcta gttctggatc gagctcagga
     3961 ttgagccctg agtctggcgg agtgctggat tatctcccgg ccttttttga tcaagcgcct
     4021 gcaaatagca agcttaccat tgctaaggct gaagagctca catgggatgg ccaatggctt
     4081 tcggcctgga ggattctcga aaaagcggat cccaataatc gcgatcctta cgttcttaca
     4141 gaaaaaatac gaacaatgct cgatggcaat acctctctcg atttatttca gggcttttcg
     4201 atcgccgata tgccgcccga tggagattgg gaggccgctt gcgaggccgg aactctagac
     4261 gaggaatatt tcgatttcga cccccatagt gcggtgcaag ctctcctaaa gcgcggtgtc
     4321 caagttccgc cagtgctggc gacggcgcta ggcgatttct attacagtgc atactatgat
     4381 tatgtcgaca attcttcttt gagcctcgac gagataaggg acaatgcgct ctattggtat
     4441 gacattgcga acgaggactt cattctggtc gaattgcaga gcattctgca ctatgccgag
     4501 ctccttatgg atgcgggtcg tgcggacgaa gcggttgcaa ttcttgaaga tgaaaaggaa
     4561 tgggagccag aagataccga attgagacta cagctaatag atgcatatat cgcgacagag
     4621 aacaattcaa gcgccttttt ggagctcgat gggctaatag agagggcagc ttcgaaagaa
     4681 gatgcacgcg aattctaccg tagagcattc gatttggcac tcgagataca ggatcagcaa
     4741 gcgctgaatc gatacctagc ggcattcgag aagaaatatc ccaatgactg gcttgctggt
     4801 cttgtgcgtc acaaagtggc tctccagatg ggcaactaca cggctgctca gacaattgcc
     4861 gatggactta tgaagcaatt tcctctcaag accgagcttc tcgagggtct tttgagcaac
     4921 tggcttgatt atttggacta caattccgcg attgccgaag cgggacttgc ctttcttgac
     4981 cgttgggtgg tcgcgtctca gtggaatcct gtagcgctgg gaacttattg tctctatagg
     5041 tctctctatc gctatatgat aatacagaat tggcctcctt cctatatgaa aaatgttgct
     5101 atccagaacg cgtttacaga tctcaatata gctgagatga atttgtcggc agcaagaaat
     5161 gcgctttccg aagaaggcaa ggctcttttg gatatgatac cagatttccg aaatgaactt
     5221 cagtctgcaa ttgggtacta aagttgctaa gcggtcgcgt tcatctagat tttgggtctg
     5281 gataagcaaa ggcgctctac aagtagaggc gcgctacaaa tagacgcgct acaaatagag
     5341 gtgctctaaa atcaaagacg ctctgccaga cgattaaaat cgagcttgtt gttttcgagc
     5401 acttcgctga caattgcctc tgggatattt agagactggc cttgcgccag ggcgtatgca
     5461 gcgcttaagg ctccggcaag agctgctatt gtatcggtat ccccacccac agaggctccc
     5521 attcggatgg aaagccagac atcgtccata gaagccagga aaatacacat tgcggcagct
     5581 gccacatcta ccgaggttag cgtggtccca taaatatagt agatgaagtc caaaatctcg
     5641 tcggtatccg agaaagaaga agctaacttt ttgaaatgca gaagccgcgc catgatcgag
     5701 gcaccacact gagggtagcc ttgagaatct ggcgccgacc gagctgcttc cgcgcccttt
     5761 tttgcctccg acaagatctc ttcgatattc tttccgtaga tagccgcaag gagcgcgtag
     5821 ccataggcca tcgccggctc catggctagc tgggtgttgt gagtgggaat gaggcatgct
     5881 cgcacacaat cggcaagtgg cctttgcctc gctgcggcga atagcacggc tgcagggcta
     5941 cgcattacgc ctccgcaggt tgtgcctttc tttcctgctt cttcgggaga ctcccctcgt
     6001 tcgatagctt cgagtgccaa tctggagcta ggaccgatgt atcccttctc tatggcattg
     6061 ctatctcgca tccagcggag gagtcttttg gcagtgatat aaggatcgat cgaatccttt
     6121 tcggaatatt cttccaaaag agtgcagacc tgctcggtgt cgtcggttat gcttgccttg
     6181 ggcaggtttg ggtggttttt ggattgggaa ggctcaagca gcctatcgac gagtccaaac
     6241 cgactagata tttcggctcg agtcatgaat tcggtgggca tgcccatcgc atcgcctacg
     6301 gcataagcga tgagtatccc gcgaataagg tttagagggg gcttactggg catgctttca
     6361 agctcgggct gcatcactta aacctcctca acctccagaa gtcacagatg tcgaaccgaa
     6421 gagccccgaa acgaagtcgt aggcgagagc gagcttctct tccggctctg caagataata
     6481 tcgatagact ggctcgagag tataataacc agcataagcc tttatcttta aggcattttt
     6541 tatagcttgc cagtctgtat cgccttcgcc ggccggcagg tgcttttttt ctcccgaatg
     6601 atcgctcaag tgtagatgta cggggctcac ctttaggatg aattccgcgg gatcgaggcc
     6661 ggcttcacat gcttcggttg tgtcgaccat aggttttatc gaagaattgc caagcgattc
     6721 gatgtattcc agcaatcctt cggccgtccg tccggcgcct ttcgggctat tctccacagc
     6781 taggacaatc ttcgctttat cgcattgttc cgcaagatga agaatatttt cccgaatagg
     6841 ctgttctatc gagctcttga gctgtacttc ggtttgaatg tcatggggat gaatgaccag
     6901 aacctctgcc cccagtgccg cgcagagtct tacaaggcct tcggcgaatg ctcgttcctc
     6961 gcttatcatt ccggggcttg cattatagag catcgagaac atggcgagtg gataatgcac
     7021 cgaggcgaca tggattttgc tccgctctgc cttgagggca aaggattccg aggcatccgc
     7081 aaagcactga ggcatcaact cggcgtgcag aaagcccgcg cccgctatga gtcgtaggct
     7141 ctctgcagcg cttcttgggt agagcgcggc cgtggaaaaa gaatacttat ttgcggcctt
     7201 tgcattaaag gctttttcat caggtgtcat ttcatcagcg gcctttgcat cagaagtctt
     7261 tgcggtctca gccatctatt tccgcctgca gttcctttgc ggccttgtct agggctgcct
     7321 ttggcgtaag cgcccctctt tctagggcat cgacggcctg cgcaaggagc tgatccattg
     7381 ttcccatctg ctcgaagtgc caataggacc agcttatatc caactgatcg aaggctatgg
     7441 catacattgg attctcggtt accgctgcct tggcctcggg cagagtcagc ccggacttgc
     7501 gtatcggcaa gtatccggtg ttgagcgcaa agctcgtctg attctctttg ctggcaagga
     7561 attttacgag gttccaggca gcttctctta tttttggatc ctggctcaaa ataacgagga
     7621 catttccgcc aagggcgacc gatgggcgct tgaagtatgg catcggtatt gcaccgatct
     7681 ttagattggg gtctttggac cagcggctta tccttgccga agtagcggcc aagaacgcga
     7741 gatttcccgc gaggaatttt ttctctgcgt tggagtgttg gccaaccggc attacctttt
     7801 catcgacgaa tctcttccag aaattggcga cttcgacgcc tttatcgtca ttgaacgctg
     7861 gcgatatctt tgtgcctttt accgagataa cgctgttttc attctgattc agcatggtct
     7921 tgaaaagcca gggtacatct gttacgtcga agccccagaa atcgggcgtt cccgtgatat
     7981 ttccctttgt ctgggcggtt tttgccactt gcagaaattc attccaggtt ttgggcggat
     8041 tcttaaggtt aataccggct ttttcgatta tcgctttgtt gtagtacatc acctgggtag
     8101 agataccata gggaaccgca cagattctgc cattgtattt tgcatagtcc catactcctg
     8161 ggtagatgtc atccttgttg aagtcggggt cattcaactt ggattcgatg aagaaatcgt
     8221 ctcgagcgcc ggtatagaga ggccctgccg ccattagtgc tacatcagga gcggtgtttg
     8281 aaagtagggc ggcgctcact tttgtcgctg tgtccgcata gtttcccgaa taggagtatt
     8341 ctatcgagac atttggattc gctgccttgt atttttcgag gagcgctttg aatgcctctc
     8401 cggaatctcc cgaaaccgcg aaccatacct tcagcttcgt ctgcgcaccc g
//
LOCUS       DAOOWY010000080         8434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_8370_length_8434_cov_21.081752, whole genome shotgun sequence.
ACCESSION   DAOOWY010000080 DAOOWY010000000
VERSION     DAOOWY010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8370_length_8434_cov_21.081752"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /gene="rbsC"
                     /locus_tag="PK105_06630"
     CDS             <1..272
                     /gene="rbsC"
                     /locus_tag="PK105_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HOU61424.1"
                     /translation="SYVMKYITGKPDAGADLLLDSIAAVVIGGASMAGGSGTMGRTIL
                     GALVIAVVQTGLRIIGMQTFMTYILVGSILIIAVILDQVFPNLNR"
     gene            303..1058
                     /locus_tag="PK105_06635"
     CDS             303..1058
                     /locus_tag="PK105_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU61425.1"
                     /translation="MPHILEVKNITKRFGGLTAVDDVGFYVDHGEVVALVGDNGAGKS
                     TLIKVISGVYKPDAGEIFLNGSKIHIESPIDAINYGIETIYQDLALAENLDVPSNIFL
                     GREITKKKAGLIPVIHQEYMLEESRKVLDRLDIEIPSLKSNIRNLSGGQRQAVAISRS
                     IYWNAQVLIMDEPTAALGISEQKKVLELVSSLRKQGIAIIIISHQMYDVFSVADRIIV
                     MRRGIKAGERLVKETTSEEIVSLIVGAETVEKK"
     gene            1061..3208
                     /locus_tag="PK105_06640"
     CDS             1061..3208
                     /locus_tag="PK105_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU61426.1"
                     /translation="MKFFTRTFNSFLIIILLQAVLVIVFVSGRVSKSQEADFHQELKD
                     EALNVYDNFNAWKRVLWGAIVDLNQSQQLRKIADAQPAGDLPPDLIAMIQKAAALANC
                     EFITVRNNYSSKTVAIKLSDDILPAPNVEDLCIDRPHPYIEMLFSNGALYFTGNLRIT
                     TSHDQSIDIFLAKIVNPGLMTQLSFNKKTKSVACFDHYLVGNAALGSDFLDMMENMKF
                     ENAYALINQFFIEGETYGLILQQSGTVKFCRNNHELRKTLFVCTFLSRKDYKARITFI
                     NRSVLLISILVAIFTIAMSVGLSKAVTDPIRQLRNAMVGLKRGERITRLPTPHTEEIA
                     DLFHGFDEMSVQIAQDKQELSLQLNEITHIKDYTDTIFDSIQEKILVINSAFIIEKAN
                     QSFLNYCGKAENMVVGCNIDEVSLALFDEPIHASIRAIITAAKPMDTQIRRSTHGHSF
                     EIKFYPLLDKERDPPSINCIMIIEDVTARLAYEEKIRLAEKLASLSMLSAGVAHEINN
                     PLSSILINIQNLISTEYVPERLEDLKLIEQETKRIARIVHNLLEFASCSTKVERITDV
                     NYSVEKVLRLLGYSIKKESSIIISTELSASLPHAAIREDEFNEILINLIKNAIEAIGS
                     EGQITIKTIYLQEKSMVECSVDDTGQGIPESILPRIFDPFFSTKGDYGNSGLGLSIVY
                     GLVQEFNGEIEVESKEGYGSCIRIRLPVANLSL"
     gene            3233..4630
                     /locus_tag="PK105_06645"
     CDS             3233..4630
                     /locus_tag="PK105_06645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU61427.1"
                     /translation="MTTVLIVDDEEGIRHGLSRFLEREGYAVELAEDSVHAESILKNK
                     KIDCAILDLRLKEPITGLDLLAKIKEEDPEIPVIIITAYGSIESAIEAMKRGASDYIL
                     KPIDNESLLTLIKRNLEIAQLRRDNRYLKNELLQKEYQQNIITKSSVMLEILAKLNSV
                     KDSTASILITGESGVGKEVFARYVHFTSIRNSGPFVSINCAALSEDLLLSELFGHEKG
                     AFTGAIERKIGKFELANGGTLFLDEIGDMSLSTQAKLLRVLEENSFERVGGTKRINVD
                     IRIITATNHKLHELIRDGRFRSDLYYRIATIEIYLPPLRSRKEDIPYLAEHFIQHYAE
                     RYHKQVEGLSPEVLQRWLSYDWPGNIRELQNVVHQSVLLCTGSRIEVDCLLDGLIDAN
                     NGHSQIQIDTEETNGPLHSLKEGSQRVIEKFECQQIQLALSLTKGNKSKAARMLGITR
                     KTLLDKMKKYSLLST"
     gene            4646..5833
                     /locus_tag="PK105_06650"
     CDS             4646..5833
                     /locus_tag="PK105_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU61428.1"
                     /translation="MLTIKGTGCALIDYLYSDIRLDSEVFKRYRSKKSGDGGLEPGKL
                     VFVENLERFADRPFSEILNNLTGGRKPDRANIGGPSMVALIHAAQMLEGHKVQVQFYG
                     ARGNDESGQALMQIVEQVPLDTSDYIVLDGETPFTQVFSDPTYDNGHGERTFINGRGV
                     ADLYNADFLTSSFFDADIVAFGGTALVPEIHDKLASLCRHAQHAGAIVVVNTVYDFLC
                     ERKNPGYCWSLGGEEAAYPWIDMLIVDREEGMKLSGAKNIKEALAFFKAQGTSAAIIT
                     DGARDIHFYSDGRLFRPCEASLAVSEEVTRILKSPDKPQGDTTGCGDNFVGGVLASLA
                     LQSEAGCRRGSFDIIQACEMAIVSGGFACFYLGGTYLERYPGEKQENVMHLLDLYHRQ
                     RQG"
     gene            5838..7040
                     /locus_tag="PK105_06655"
     CDS             5838..7040
                     /locus_tag="PK105_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOU61429.1"
                     /translation="MKYFVQFGAGNIGRSFIGQLFSRAGYEVIFIESALPLVRIINKK
                     REYRIIIKRNDTPDEEIVIRNIQALHIDETQAVRNAILKADYVATSVGASALSAVAPI
                     LADGIEFRFVQRPHKPIDIIIAENIRYGAEYLKNLLSARLPEGFPLDAYVGLVETSIG
                     KMVPIMRKEDIANDPLMLFAEKYNELIVDRNGFKTEIPALSTITPVDNIKAYVDRKLF
                     IHNLGHSAIAYIGYANFANDPCLQYIWQALEKPDIANKAHAAMTQAAVALAKEYPNDF
                     EISSLNAYIEDLFVRFSNKALGDTIYRVGRDLYRKLAHDERIIGAMILAAQHGLPFDA
                     IAQVFSAALRFRATDEEGALFPRDAEFKAYLSSHDIDSMFREVCGLSLQDPVESIVYD
                     RLVQYVKK"
     gene            7079..8272
                     /locus_tag="PK105_06660"
     CDS             7079..8272
                     /locus_tag="PK105_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61430.1"
                     /translation="MPHEKTLIDQALTVGKGVLRLEPNWVPRAFCVPGRRLRLHPNDY
                     YAYGPRRGGIDERWFSSTTKADNGPLTTTYEGLSFIWIDETHHVLLKDAIDTMGNEIL
                     GENIMKKYGGWRAYSKFFDNLGPLPLHLHHTDKMAAHIGQLGKPESYYFPTQLNNHGG
                     AFPYTFFGLNPGTTKDQIKACIADWEKRDNNILALSRAYMLKPGTGWDVPPGILHAPG
                     SLLTYEPQRASDVSAMFQNIVWNAYTAKELLQKDIPEEKRDDLDYYIELLDWELNIDP
                     LFYEHRFMEPKPVKPLEEMRAQGYEEYDIVYKNEYFSAKELTILPGRSALIKDKGPYG
                     AIVVQGHGSFGSLTIESPAMIRFGQMTQDELFVTNNTAKEGVLITNTSSTDALVMLKH
                     FGPAA"
BASE COUNT         2510 a         1691 c         1932 g         2301 t
ORIGIN      
        1 ttagctatgt gatgaaatat attacaggaa aacctgatgc aggggcggac cttcttttgg
       61 atagtattgc ggcagtagtt attggaggag caagcatggc tggaggttcg gggacgatgg
      121 gaagaacaat acttggcgct cttgtcattg cagtggtaca gactggtttg aggattatag
      181 gcatgcagac gttcatgacg tatatccttg tgggttcaat attgattatc gcagtaatat
      241 tggatcaagt tttccctaat ctcaatcgct agatgattga tcataaaaag cagggaggag
      301 ctatgcccca tattcttgaa gtaaaaaata tcactaaacg ctttggtggt ttgactgctg
      361 tagatgatgt aggtttctat gtcgatcatg gggaagtggt cgcacttgtt ggcgataatg
      421 gtgcaggcaa gtctacattg atcaaggtca tttcaggtgt atataaacct gacgctgggg
      481 aaatatttct caatggctcc aaaatccata ttgagtcgcc aatcgatgcg atcaactatg
      541 gtattgagac aatctatcaa gaccttgctc ttgcagaaaa tctcgacgtt ccttcgaata
      601 tctttcttgg tagagaaata acgaaaaaaa aagctggcct tattcctgtt atccatcagg
      661 aatatatgct tgaagagtct cgcaaagttc tcgatcggct tgacattgag attccttcac
      721 ttaaatccaa tatacgcaac ctatctggag gtcagcgaca ggccgttgcg atttctcgat
      781 cgatatactg gaatgctcaa gttcttatta tggatgaacc tacggcagcg cttggtatat
      841 ctgaacagaa aaaagtactt gaattggtga gctcgctgcg aaagcagggc attgcgatta
      901 tcattataag ccatcagatg tatgatgtct tttcagttgc tgatagaata atcgttatga
      961 gacgaggaat taaagccggt gaacgacttg tgaaggaaac gacaagcgaa gaaattgtga
     1021 gcttgattgt tggagccgag acagtcgaaa aaaaatagcg atgaagtttt ttacacgtac
     1081 cttcaattcg tttctcatca ttattctatt gcaggcagtg cttgttattg tgtttgtgtc
     1141 aggacgcgta tctaagagtc aagaggcaga ttttcaccaa gaactaaaag acgaagccct
     1201 caatgtatat gacaatttta atgcatggaa aagagttcta tggggcgcta ttgtcgatct
     1261 caatcaatct caacagctga ggaagatcgc ggatgcgcag cctgcagggg atcttcctcc
     1321 tgatctgatt gcaatgatac aaaaagctgc agctcttgcg aattgtgaat tcatcactgt
     1381 aagaaataac tattcctcca aaacagtcgc gataaagctt tctgatgata tcttgcccgc
     1441 gcccaatgtc gaggatttgt gtattgatag accccatccc tacatagaaa tgctcttttc
     1501 aaatggcgct ctgtatttta cagggaatct acgcattacg acatcccatg atcaaagcat
     1561 cgatatcttc ttagctaaaa tagtaaatcc tggattgatg actcaattga gtttcaataa
     1621 aaaaacaaag tcagtagctt gttttgacca ttatcttgta ggaaatgcag cgcttggatc
     1681 agattttcta gatatgatgg aaaatatgaa atttgagaac gcatatgcgc ttatcaatca
     1741 attttttatt gaaggagaaa cctatgggct tattcttcag caatcgggca cagtaaaatt
     1801 ctgcagaaac aaccatgagt tgagaaaaac tctttttgtc tgtacctttc tttcaagaaa
     1861 agactataaa gcacgcatta cattcatcaa tcgttcagtt ttactgatca gtattcttgt
     1921 tgctattttt actattgcga tgagtgttgg gttgagcaag gcagttacag atccaatacg
     1981 tcagcttaga aacgccatgg ttggtcttaa acgtggagaa aggataacta gactccccac
     2041 gccgcacaca gaggagattg ctgatctttt tcatggcttt gatgaaatgt cggtgcaaat
     2101 tgcacaagac aaacaggaac tttcgcttca actcaatgaa ataactcata tcaaagacta
     2161 tacagatacg atattcgatt caattcaaga gaaaatcctt gttatcaata gtgcattcat
     2221 aatagaaaaa gcaaatcaat cattcctcaa ttactgtggg aaagccgaaa acatggttgt
     2281 tgggtgcaat attgatgagg tttctcttgc gcttttcgat gagccgattc atgcaagcat
     2341 tcgagccatt ataactgctg cgaaacctat ggatacccag atacgacgta gcactcatgg
     2401 ccattctttt gaaatcaaat tctaccctct cctagataag gagcgggatc ctccttcaat
     2461 caattgtatt atgatcatcg aagatgtaac tgcacgcctt gcttatgaag aaaagatccg
     2521 ccttgctgaa aaactcgcga gtcttagtat gctttctgca ggggttgcac atgagatcaa
     2581 taatcccctt agttccatct tgatcaatat tcagaatcta atcagcactg aatacgtgcc
     2641 agagcggctt gaagatctca agcttataga acaggaaacc aagaggattg ctcggattgt
     2701 acataatctt ctagaatttg catcttgttc cacaaaggtt gagcgcatta ctgatgttaa
     2761 ttattccgtt gaaaaagtat tgagactatt aggctactca ataaagaagg aaagctccat
     2821 catcatatcc acggaattaa gcgcatcatt gccccatgcg gcgatacgag aagatgagtt
     2881 caatgaaatc cttatcaacc ttatcaagaa tgctattgaa gctataggtt cagaagggca
     2941 gataaccatt aaaacaatat atctgcagga aaaatctatg gtagaatgca gtgttgatga
     3001 tactggccag ggaatcccgg aatccatttt gccaaggatt ttcgatcctt ttttctcaac
     3061 aaagggagat tatggcaata gtggactagg cctttcaata gtttatgggc tagttcaaga
     3121 attcaatggt gagatcgagg ttgagagcaa agaaggatat ggatcttgta tacgaatacg
     3181 attgcccgtt gcaaatcttt ctctctaggt tttacctaaa agaagggtgt aaatgacaac
     3241 tgtcctaatc gtcgatgatg aagagggcat tcgccatggt ctttctcgct ttcttgagcg
     3301 agagggttat gcagttgagc ttgcagagga tagtgtacac gctgaatcta tattgaagaa
     3361 taagaaaatt gattgtgcaa tactcgatct tcgtcttaaa gagcccataa ctgggctcga
     3421 tttgctcgct aaaatcaagg aagaagatcc agaaataccc gttattatca ttactgcata
     3481 tggaagcata gaatccgcaa ttgaagcaat gaaacgaggg gcgtcagatt acatattaaa
     3541 accgatagac aatgaatcgc tattgactct cataaagaga aatctcgaga ttgcgcagtt
     3601 aagacgagat aatagatatc taaaaaatga attgcttcaa aaagagtatc agcaaaatat
     3661 aataacaaaa agctctgtca tgctagaaat tcttgcaaaa cttaatagtg tcaaagacag
     3721 tacagcttcg atccttatta caggggaaag cggagtaggt aaagaggtct ttgcccgcta
     3781 tgtacatttt acatctattc gcaactctgg gccatttgta agcatcaact gtgcagcgct
     3841 ttcagaagac ctcttactca gcgaactttt tggtcatgag aagggtgcct ttactggcgc
     3901 aatcgaacga aaaattggca aatttgaatt ggcaaatggt ggcacccttt ttctcgatga
     3961 aattggcgac atgtctcttt cgacacaagc aaaactcttg cgagtgctcg aagagaatag
     4021 ctttgagcga gtgggcggaa caaagcgaat caatgttgat attaggataa tcacagcaac
     4081 gaatcataaa ctccatgaac tcattcgaga tggtcgattt agaagcgatc tttattatcg
     4141 cattgcaact attgagatat atttaccgcc attgcgaagc cgcaaagaag atattcccta
     4201 cctcgcagag cattttattc aacattatgc agaacgatat cacaagcagg tagaaggtct
     4261 aagcccagaa gttctacagc gatggttatc ctatgactgg ccaggcaata ttcgcgagct
     4321 acaaaatgtt gtgcatcaat cggtattgct atgcacaggc agcagaatag aagtcgattg
     4381 tttgctagat ggcttgatag atgctaataa cgggcattct caaatacaaa tcgataccga
     4441 agaaacgaat ggtcctcttc actccttaaa agaaggatca cagcgggtga tagaaaaatt
     4501 tgaatgccaa cagattcagc tagctctctc ccttaccaaa ggaaacaaga gcaaagccgc
     4561 gcgtatgcta ggaattacaa gaaaaactct tttagataaa atgaaaaaat attcgctatt
     4621 atcaacatga gctaagaaga attaaatgct gactataaaa ggaactggtt gcgcccttat
     4681 agattatctc tattcggata ttcgattgga ctctgaggtc tttaagaggt atcgatcgaa
     4741 aaagtcaggg gatggcgggc ttgagccagg aaaattggtc tttgttgaga atttggaacg
     4801 gtttgcggat agaccttttt ctgagattct gaacaatctc actgggggga gaaagccaga
     4861 tcgcgccaat ataggaggac cttcaatggt cgcgctcatc catgcagcgc agatgcttga
     4921 aggtcacaag gttcaggtgc aattctatgg cgcgcgtggc aatgatgaaa gcgggcaagc
     4981 cttgatgcag attgttgaac aggtccctct agataccagc gattatatcg tattagacgg
     5041 tgagacgccc tttactcaag tattttctga tcctacttat gacaatggcc atggcgaaag
     5101 aacttttata aatggtagag gagttgccga cctctataat gcggatttcc tgacttcaag
     5161 cttttttgat gctgacattg tggcatttgg cggaaccgcg ttagtgcctg aaattcatga
     5221 caagcttgca tctttatgcc gccacgctca gcatgcgggc gctatcgtag tggtcaacac
     5281 agtgtacgat tttttatgtg agcgaaaaaa tccaggatat tgttggagcc ttggaggtga
     5341 agaagccgca tatccatgga tagatatgct tattgtggat cgcgaggaag gcatgaaact
     5401 cagtggtgca aagaacatca aagaagcact tgcattcttt aaggcgcagg ggactagtgc
     5461 tgcgattatc actgatggcg cgcgggatat ccatttttat tctgatggca ggctttttag
     5521 gccatgtgag gcgtcgcttg cagtatctga agaggttaca agaatattga agagtcccga
     5581 caagcctcag ggagatacta ctggctgtgg ggacaatttt gtgggaggag tgctcgcttc
     5641 tctagccttg cagagcgagg caggctgtag aagaggcagc tttgatataa ttcaagcctg
     5701 tgaaatggcg attgtttcag gcggttttgc ctgcttctat ttgggaggga cctatctcga
     5761 gcgctatcct ggcgaaaagc aagagaacgt catgcatcta ctcgatctct atcataggca
     5821 gcgtcaaggt tagactgatg aagtattttg tacaatttgg cgcaggaaat attggccgct
     5881 cctttattgg ccagcttttc agcagggcgg gctatgaagt aatttttatc gagagtgctt
     5941 tgcctttagt ccgcataatc aataaaaaaa gagaatatcg cattattatc aagagaaatg
     6001 atacacccga tgaagagata gtaatccgca atattcaggc gctccatatc gacgaaacgc
     6061 aagcagtgcg taatgcgatt cttaaggcag attatgtggc aacgagtgtc ggggcctctg
     6121 cgctttcagc ggtggcacct attcttgcgg atggcatcga atttcgtttt gtccagcggc
     6181 ctcataaacc aatcgatatc atcattgctg aaaatattcg ctatggagct gaatatctta
     6241 aaaacctgtt gagtgcgagg ctgcctgaag gtttccctct cgatgcctat gtaggacttg
     6301 tcgaaacctc gattggtaag atggtgccca taatgcgtaa ggaggacatt gcaaacgatc
     6361 ctcttatgct ttttgctgaa aaatataatg agcttattgt cgataggaat ggatttaaga
     6421 ctgagatacc agcgctttcc acaattactc ctgttgataa tatcaaagca tatgtggacc
     6481 gaaaactctt tatccacaac ctcggacatt ccgctattgc ctatattgga tacgctaact
     6541 tcgcgaatga tccctgcctg caatatatct ggcaggcgct tgagaaaccc gatattgcta
     6601 acaaagctca cgcagcaatg acacaggcag cagtagccct tgccaaagaa tatccaaacg
     6661 attttgagat ttcctccctc aatgcatata tcgaggattt gtttgtgcga ttttcaaata
     6721 aggctttggg cgataccatc tatcgggtgg gacgcgacct ctatcgaaag cttgcgcacg
     6781 acgaaaggat tattggggcc atgatacttg cggctcagca tggtctacca ttcgatgcga
     6841 ttgcacaagt gttcagcgca gcacttcgct ttagagctac ggacgaagaa ggtgctctgt
     6901 ttccgcgaga tgccgaattt aaggcctatt tatcttcaca tgatatagac agcatgttca
     6961 gagaagtgtg tgggctttct cttcaagatc ctgtggaaag catagtgtat gatcggcttg
     7021 ttcaatatgt gaaaaaatag agcatgattg aattcgttat attgaaaggg ggcttcaaat
     7081 gccacatgaa aaaacgttga tagatcaggc gcttactgta ggaaaaggtg ttcttaggct
     7141 tgagccaaac tgggtaccgc gcgctttctg tgttccaggc aggaggctta ggttacatcc
     7201 caacgactac tatgcgtatg ggccccgccg cggaggaata gatgaacgat ggttttcgtc
     7261 aacaacaaag gcggacaacg gaccgctaac aacaacctat gaaggtctct cgtttatctg
     7321 gatcgacgaa actcaccatg ttctcctaaa ggatgcgatt gataccatgg gcaatgaaat
     7381 ccttggcgag aatatcatga aaaagtatgg aggatggcga gcttattcaa aatttttcga
     7441 taacctaggt cctttgcccc ttcatctgca tcatacagac aagatggctg ctcacatagg
     7501 acaattaggt aaacctgaat cctattattt tcctacacaa ctcaataatc acggaggtgc
     7561 cttcccctac accttttttg gcctcaaccc aggcacaacc aaggatcaaa taaaggcatg
     7621 cattgcggat tgggaaaagc gcgataacaa tatcctcgcg ctctcccgcg cctatatgct
     7681 caagccaggt actggatggg atgtgcctcc tggaattctg catgcgcctg gctcgctttt
     7741 gacctatgaa cctcaacggg cctcggatgt ctcagcaatg tttcaaaaca tcgtgtggaa
     7801 tgcatatacc gcaaaagagt tgcttcaaaa ggatatccca gaagaaaaaa gagatgacct
     7861 cgattattac atcgaactgc tcgattggga actcaacatt gacccacttt tctatgaaca
     7921 ccgattcatg gagccaaaac ctgtaaaacc cctcgaagaa atgcgcgcgc aaggctatga
     7981 agaatacgat atcgtctata agaatgaata cttctctgcc aaagagctta cgatattgcc
     8041 aggtcgctca gcgcttatca aagataaagg accctatggc gcgattgttg tacaaggcca
     8101 cggcagcttt gggagcctca ctatagagtc accggcgatg attcgctttg gacaaatgac
     8161 tcaggatgag ctatttgtca caaacaatac cgctaaggaa ggagtgctca tcacgaacac
     8221 aagcagcacc gatgcgcttg taatgctcaa gcactttggg ccagcagcat gagagagaaa
     8281 aaagaaaaag atacgcattg aaaagatagc ctgtttggaa ggcaggggga gcattctaag
     8341 caacagaatg gtgtgcgcgg tattcgttta cttctgagac ttgctctggc gaggaaccgg
     8401 taggtactcc actcctcctt gttgttttac tggc
//
LOCUS       DAOOWY010000081         8366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_8452_length_8366_cov_9.636025, whole genome shotgun sequence.
ACCESSION   DAOOWY010000081 DAOOWY010000000
VERSION     DAOOWY010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8452_length_8366_cov_9.636025"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PK105_06665"
     CDS             <1..417
                     /locus_tag="PK105_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61431.1"
                     /translation="SEIVAKEEENSQEAPEAPVAPEAPAAPEAPVAPEAQEAPAALEA
                     PKVTLPTFSYSDTKEGMFRRSLVISIGLFPFSYFYTNFAFDLARYISHGFDTAYAPWP
                     FNTQYGVALTNSEVWTRIGIASGASILLGFLSVIIE"
     gene            505..1065
                     /locus_tag="PK105_06670"
     CDS             505..1065
                     /locus_tag="PK105_06670"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOU61432.1"
                     /translation="MANGSDFRGDYQEFNLDDAIRKIPDFPKPGILFYDITSILANPR
                     AFKYCIDSMFKLYGDTEFDAAAAIESRGFVFAAPFCYERNIPLLLVRKKGKLPGRTIS
                     CAYELEYGTAVLEMHIEDIKPKARFLIIDDLIATGGTINATCEMIKRGGAVPVRAFSV
                     IGLPSLKYQEKVTDVGIDTLIEYSGE"
     gene            1144..2814
                     /gene="gpmI"
                     /locus_tag="PK105_06675"
     CDS             1144..2814
                     /gene="gpmI"
                     /locus_tag="PK105_06675"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOU61433.1"
                     /translation="MLERLKKNPNWKNREGPLVLVIMDGVGYGTYKEGDAVLAARMDH
                     FRELEANCPKTRLKAHGTAVGLPSDDDMGNSEVGHNAIGCGRVFAQGARLVNISIESG
                     IIFEGKVWRELIANIKETGGTLHFIGLFSDGNVHSHIDHLKAMLVQAQREGIKRARIH
                     ILLDGRDVGSQSALEYVLPFETFLEEFRNQGSDYRIASGGGRQYITMDRYGANWQMVK
                     RGWDCHVRGIGRQFASAEEAISTYRKEIPGIIDQDIPEFVIAENGRPIGTIEDGDSVI
                     FFNFRGDRALEITAAFEQDNFDKFDRVRRPKVYYAGMMQYDGDLKVPSNFLVSPPAID
                     RTLGEYLAYTGLKTLAISETQKYGHVTYFFNGNRSGKFSEELEDYVEIPSDRVPFEQR
                     PWMKCAEIADYVINAIENGPYRFIRLNFPNGDMVGHTGVFQAVVCGLEGMDIQLGRIY
                     EAVRKKGGILLITADHGNSDDMYEHDKQTGRVILGPDGKPKAKTSHSLNSVPCVIYDP
                     QYQGEYKKELRQGMGISSLAATCAELLGYIPPDHYDPSVLDMPIENEA"
     gene            2822..3382
                     /gene="cyaB"
                     /locus_tag="PK105_06680"
     CDS             2822..3382
                     /gene="cyaB"
                     /locus_tag="PK105_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOU61434.1"
                     /translation="MQNPEYSHNLEIELKARVQQREEVESRLSSFMHYIGPINKLDEY
                     WEVSNARLGDFGGFRFRVRHELASTTITFKEKTFDGDVEINHEFEFSVNGEPAFKAFI
                     EKMHAHLIYQKQKRGTRWEDGTGLVAEVVEVSSLGLFLEVECIGDSRVTLQQEEAKSR
                     LYRVIDQCGISRSALEPRPYSQLLGY"
     gene            complement(3653..4237)
                     /locus_tag="PK105_06685"
     CDS             complement(3653..4237)
                     /locus_tag="PK105_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOU61435.1"
                     /translation="MKKASVILISILVMTSCATVSDVTTLKRCEFRMQGIKDVVAAGV
                     NISGKKSISELSLLDAGRITLAIKKGSLPVTMTLAVEIRNPNTQTVAVDRVDYAVALD
                     GESLFSGFTTDSVKVPGGNRVAVIPLKVTFDLFSLKEDNTQDAILNLLFNLAGASEIP
                     STLTLQLKPSISIGKAMIPYPGYMEISREFGGRQ"
     gene            complement(4355..4513)
                     /locus_tag="PK105_06690"
     CDS             complement(4355..4513)
                     /locus_tag="PK105_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61436.1"
                     /translation="MRIIYKMNGKGFVGSLVNLPAAYFRGRIIKETSSKIEREIRAYK
                     SWLENKAV"
     gene            complement(4779..5249)
                     /locus_tag="PK105_06695"
     CDS             complement(4779..5249)
                     /locus_tag="PK105_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOU61437.1"
                     /translation="MSLERAKEHLKKWNKDEQVRELDESSATVELAAKALGIEQARIA
                     KTLAFMVDDSAILIVAAGDAKIDNRKYRQLFGTKAKMIDKDLVERYIGHNIGGVCPFG
                     INENVRVYLDVSLKRFTTVYPACGSSNSAIEMTCQELEEIASAEKWIDVCKTEV"
     gene            5506..5652
                     /locus_tag="PK105_06700"
     CDS             5506..5652
                     /locus_tag="PK105_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61438.1"
                     /translation="MRTRLHLFDNLGFHAQPGSGINLEDKAIAGFYLDIVLEHKRGLL
                     HGNG"
     gene            complement(5655..6539)
                     /locus_tag="PK105_06705"
     CDS             complement(5655..6539)
                     /locus_tag="PK105_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOU61439.1"
                     /translation="MNNQSDEKLLNDVLRFQGEEITGSLLYEKLAKECKDEHNAAVLF
                     DMAEAEKRHYAFWKSISGQEVAPSRGRIMFFTLLAKVLGLTFTLKLLEKGEEVTAADY
                     ARVASIHPGAEKMGEEEEQHEDALLAMIDEERLSYVGSIVLGLNDALVELTGTLAGLT
                     FAFQKGKLVAVSGIITGIAAALSMAASNYLASKADNDPKAKKAALYTGGAYLITVILL
                     VLPYLIVPQTGFWIYASLGITLLIAVGIIAGFNFYVSVARSEAFKPRFLEMAGISLGV
                     AFLSFLVGLLVRSVVGAE"
     gene            complement(7075..7431)
                     /locus_tag="PK105_06710"
     CDS             complement(7075..7431)
                     /locus_tag="PK105_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOU61440.1"
                     /translation="MRYCWTTIIVKDLERSLAFYCDIVGLPIVRQLSGAHGNIVFLGD
                     GDTNIELIENPHAAVVTGNRISLGFAVDSLDAMLEFVASKKIDVESGPFRPNEHIRFF
                     YVLDPDGVRVQFSESM"
BASE COUNT         2278 a         1856 c         1877 g         2355 t
ORIGIN      
        1 tcagaaattg tcgctaaaga agaagagaat tctcaagaag cgccagaggc gcctgtagct
       61 ccggaagcgc ctgcagcgcc agaggcgcct gtagctccgg aagcgcaaga agcgcctgca
      121 gcgctagaag cgccaaaagt gactctacca actttttctt atagtgacac taaagaaggc
      181 atgtttaggc gcagccttgt gatctctatt gggctttttc ctttcagcta tttctatact
      241 aattttgctt tcgatttggc tcgctatata tcgcatggct tcgatacggc ttacgcgcct
      301 tggccattca atacccaata tggtgttgcc ctgacaaata gcgaagtatg gacaagaatc
      361 ggtatagctt ccggagcaag catattgctt ggtttcttga gcgttatcat agagtgatat
      421 acaaaaagca ctgtattctg tatactgcag cgtaaccttt ctataaggta gcgccataat
      481 ttccattttt gagagaggct aaatatggcg aatggcagcg attttagagg cgattatcaa
      541 gaattcaacc tcgatgatgc aatccgcaaa attccggatt ttcccaaacc gggcatattg
      601 ttctatgata ttacaagcat ccttgctaac ccaagagcat tcaaatattg tatcgattca
      661 atgtttaagc tatatggaga tactgaattc gatgcggcag ctgctattga atcccgagga
      721 tttgtgtttg ccgcaccctt ctgttatgag cggaatatcc ctctgctttt agttaggaaa
      781 aagggcaaat tgcctggaag gaccatttcc tgcgcctatg agttggaata tggaactgcc
      841 gtgttggaga tgcatattga agatatcaag ccaaaggctc gttttctgat tatcgacgac
      901 ctcatcgcta caggaggcac tattaacgct acctgtgaaa tgattaaacg cggtggggcg
      961 gtgcctgttc gcgcgttctc agtgataggc ttaccatctt tgaaatacca agagaaagtg
     1021 acagatgttg gtatagatac actcatcgag tattctggag aataggtgag taaaagaagc
     1081 cctttgggga aaagagctcc gaatttggtt tgagccaggt aataacagaa aaaggagcaa
     1141 tctatgcttg aacgattaaa aaaaaatcct aattggaaaa atagagaagg tccattagtc
     1201 cttgtgataa tggatggagt aggttacgga acttacaaag aaggtgatgc agtccttgca
     1261 gcgagaatgg atcatttcag ggagcttgaa gccaactgcc ccaaaacgcg gcttaaggct
     1321 catggaactg ccgtaggcct tccctccgat gatgacatgg gaaactcgga agtcggtcac
     1381 aatgcgattg gttgtggtcg agtttttgcc caaggtgcgc gcctcgtcaa tatttctatt
     1441 gaatcaggga taatattcga agggaaagtc tggagagagc ttattgccaa tataaaggag
     1501 accggaggaa cactccactt tattggcctt ttctcggatg gtaatgttca ctcgcatatc
     1561 gatcatttaa aagccatgct cgttcaagca cagagagaag gtatcaaacg agcgcggata
     1621 catatcttgc tcgatgggcg agatgttgga agccaaagtg ctcttgaata tgtccttcct
     1681 tttgagactt ttcttgagga atttagaaat caagggtcgg attatcgcat cgcctctgga
     1741 ggtggaaggc aatacataac gatggatcgt tatggtgcca attggcagat ggtcaagcgc
     1801 ggttgggact gccatgttag aggcatagga aggcaattcg ccagtgcgga agaggcaatc
     1861 tcgacttata gaaaggagat tccaggcatt attgatcaag atattcccga gttcgtcatt
     1921 gccgaaaatg gcagacccat cggtacaatc gaggatggag actcggttat attcttcaat
     1981 ttccggggtg acagggcgct tgagattaca gcagccttcg agcaggataa tttcgacaaa
     2041 ttcgatagag tcaggcgacc aaaggtatat tatgcaggta tgatgcagta cgatggcgac
     2101 ctcaaggttc cttccaattt tctggtttca ccacccgcta tcgacagaac gctcggcgaa
     2161 taccttgctt atacgggctt aaagacactc gctatttcag agacacagaa atatggtcat
     2221 gttacctatt ttttcaatgg caacaggtca gggaaatttt cggaagaact ggaagactat
     2281 gtcgaaatac ctagtgatcg tgtgccattc gagcaaaggc catggatgaa atgcgcagag
     2341 atagccgatt atgtcataaa tgcaatcgaa aatggcccat atagatttat tcgcctcaat
     2401 ttcccaaatg gcgacatggt agggcataca ggggtctttc aagctgtggt gtgtggtctg
     2461 gaaggaatgg atatccagct aggcagaata tatgaggcag tgcgaaaaaa aggcggaatc
     2521 ctcttgataa cggccgacca tggaaattcc gatgatatgt atgagcacga caagcagact
     2581 ggaagagtta ttttaggtcc cgatggcaaa ccaaaggcaa agacaagcca ttcgctcaac
     2641 tctgtccctt gcgtaatata cgacccgcaa tatcaaggcg agtacaaaaa ggagcttcgg
     2701 caaggaatgg gaatatcaag cttggccgca acctgtgctg aacttctggg ttacatcccg
     2761 cctgatcatt atgatccatc ggtgttggac atgcctatag agaatgaggc atagcagaat
     2821 aatgcagaat ccagaatata gccataacct ggagatagaa cttaaggcac gagttcagca
     2881 gagagaggaa gtggaatctc gcctttccag ttttatgcac tatataggac ccatcaataa
     2941 gctagacgaa tattgggaag tatcaaatgc aagattaggg gattttggtg gttttagatt
     3001 tcgcgttcgc catgaattgg ctagtactac tataacattc aaagaaaaga ccttcgatgg
     3061 agacgtagaa atcaaccatg aattcgagtt tagtgtcaat ggtgaaccag cttttaaggc
     3121 cttcatagaa aaaatgcacg cacatctgat ctaccaaaag caaaagagag gtacccgctg
     3181 ggaggatggt acaggcttgg ttgcggaggt agtcgaagta tcttcgctcg gtctttttct
     3241 tgaagttgaa tgcattggcg atagtcgcgt cacattgcag caagaagagg ccaaatcccg
     3301 cttatatagg gttatcgatc aatgtggcat ttcaagaagc gctcttgagc caagaccgta
     3361 ttcgcagttg ctagggtatt gaaagttgaa taaacaagaa ttatcgcgct gacggacttg
     3421 aatccccgac ctaccggatg aaaatcacaa gttgcacaag gtatatataa aatattgcat
     3481 tatatgctca aaactagctt cttactaatt aataactaat gattgtgtcc agtccttgtt
     3541 gatagtgtcc agtcttgttc gcgatttact gctaaaaaga tcattaggaa tcgtctccat
     3601 tacgctactg tatataataa cgcctgaacg agtcctcaag accgtattag atttattgtc
     3661 ttccgccaaa ctcacggctt atttccatat agccggggta tgggatcatc gctttaccaa
     3721 tagaaataga aggctttaac tggagggtta gtgtagaggg tatttcggat gcaccagcta
     3781 aattaaacaa caaattcaga atagcatctt gtgtattgtc ttcttttaaa gaaaaaagat
     3841 cgaatgtcac ttttaatgga atgactgcaa cacgattgcc tccaggaacc tttacagaat
     3901 cggtagtgaa accactaaat aatgattctc catcaagggc tacagcataa tctacacggt
     3961 ctactgctac agtctgggta ttcggatttc ggatttctac agcaagagtc atggttactg
     4021 gcaaagaacc tttcttaatg gctagagtaa ttctaccagc atccaaaagg ctcaactcag
     4081 aaattgattt cttaccacta atattcactc ctgcagcaac aacatcctta atcccctgca
     4141 ttcgaaattc acatctcttc agtgtagtaa catcgcttac ggtagcacaa cttgtcatca
     4201 caagaatact gataagtatg acactagcct ttttcataca acaaacctcc agatatatat
     4261 atatataata tttttcgcgc tcaattttag gcattgtcaa tcgttgagaa tgtaaaatac
     4321 cggattcagg cgctttttat tatagccttt tctttcatac tgccttgttt tccagccaac
     4381 tcttataagc ccttatctcc cgctcaatct tcgagctagt ttctttgatg attcttccgc
     4441 ggaaataggc agcaggcagg ttgacaagtg agccaacgaa gccttttccg ttcatcttat
     4501 agatgattct catgatataa catcttctta ttcgatccga atcttattcg agtaattatt
     4561 atcgatgttc atttcgtccg gtatgttgct tatctgcctc caatgatacg atctgattgt
     4621 tgaagaagta tgcaagcgct ttccaggttt gcttttacat cttctcgcct tgcttgttca
     4681 acctcacgac caacttagtg aagaaatcat ccttcattta tacatgtatt ctattatcta
     4741 tcaaggtgtc gagtccctcc ctcgttgtct tatagtcttc agacttctgt cttacagaca
     4801 tcgatccatt tctctgcgct tgcgatctct tccagctctt ggcatgtcat ctctattgcc
     4861 gaattgctgc tcccgcaggc aggatacaca gtggtaaatc tcttgagaga gacatccaaa
     4921 tacactcgta cattctcatt gatcccgaat ggacatactc cgccgatgtt gtggccgata
     4981 taccgttcga ccagatcttt atcgatcatc tttgcctttg taccgaaaag ttgtcggtat
     5041 tttctattgt cgatttttgc gtctccggca gcgacaatca atatggcaga gtcatcgacc
     5101 ataaaggcca atgttttggc gattctcgct tgctcaatac caagcgcctt agctgccagt
     5161 tccaccgttg cgctcgattc atccagctct cttacctgtt cgtctttgtt ccactttttt
     5221 aggtgttctt ttgccctttc tagactcata gatcagtatc tctcctgatt gcgattttta
     5281 cgccgtcgtc aatgggcagt atcgcgctca tgactcggtc ttcatgtatc aaaaggttgt
     5341 tgagctcctt tatataaggt atttctcaag gtctttcttt atcatctttc cgatttcgct
     5401 atgcgtcacc ttagtgagat catgccggct ctcgaaaaag aagggcatca taatcaagtt
     5461 tagcctgggt ttctggacaa caaagagccc gttgttggag ccattttgcg tacccgtctt
     5521 catctattcg ataatctcgg cttccatgcc cagccgggat ccggaataaa tctcgaggat
     5581 aaggcaattg caggatttta tctcgatata gttcttgaac acaagcgagg tcttttgcat
     5641 ggtaacggtt aagctcactc ggcgccgacg accgacctta cgagaagccc gacaaggaag
     5701 gataagaatg ctacgccaag gctaatgccg gccatctcga ggaaacgtgg cttgaaggcc
     5761 tcgctcctgg ccaccgatac gtaaaagttg aagcctgcaa tgatgccgac ggcgataagg
     5821 agggttatcc cgaggctagc gtagatccag aagccggtct gtggcacgat gagatagggc
     5881 agcaccagaa gtatgaccgt tatgaggtag gctcctcccg tgtagagggc tgcctttttc
     5941 gccttaggat cgttgtcggc cttggaggca agatagttcg aggccgccat ggacagggcg
     6001 gccgctatgc ccgttatgat accgctgacc gcgacgagct tccccttctg gaatgcaaag
     6061 gtcaggcccg ccagagtgcc cgtcagctcc accagtgcat cgttcaagcc aaggacgata
     6121 gatccgacat acgagagccg ctcctcgtcg atcatggcaa gcagagcatc ctcgtgctgt
     6181 tcctcttctt cgcccatttt ttcggcgcca ggatgaatgg aagccacgcg ggcatagtcg
     6241 gccgctgtta cctcttcgcc cttttcaagg agtttgagtg taaaggtcaa accgaggacc
     6301 ttggccagca gggtgaaaaa catgattcgc cctctgctgg gagcgacttc ctgtcccgag
     6361 atgctcttcc agaatgcgta atgtcgtttt tcagcctcgg ccatgtcgaa gaggacagca
     6421 gcattgtgct catccttgca ttcttttgcc agtttttcgt agagaagaga gccggtgatt
     6481 tcctcgccct gaaagcgaag aacatcgttc agtagttttt catcagattg attgttcatg
     6541 acatacactc ctcacaatgt tgcaattaat catacttcat cagccctcaa gactccagag
     6601 tcggaagggt ggcgaaactc cgatcgattg aaaaacctgg tgagagatca cggcctctta
     6661 tacaatttac tttgattccg agccattact ttgtgtcaca tctcgaccat atgatgggtt
     6721 ctgactcaga cctgaatcat aaggataagt tctaagtcgt cagactcttg ctatgcatgc
     6781 actcttgcgt ccgttagctg tttctaaaga gcctatatgt attgatggct ttgtcagttt
     6841 tgatggctct cagtgcttta ctgataatat taccatgcgg agatagcact aattccaata
     6901 caggatcatc gcaattcgta tattctccga aaatcggcgc ccgtagctag gttgttgtgg
     6961 aagtgaggaa gccgttttcc ggggatacgg ggatagcctt gcgccctagc atgaagtcgg
     7021 gctcaggaac tataagtcag gggccaattt gatgtttcta tatgcttttc ctggctacat
     7081 ggattcggag aactgaacac gtaccccgtc tgggtccaac acatagaaaa atcttatatg
     7141 ttcatttggt cgaaaaggac cgctctcgac atctatcttt ttcgaagcga cgaattcgag
     7201 catggcatcc agtgaatcaa ctgcgaatcc aagggagatt ctgtttcctg tcacgacagc
     7261 cgcatgagga ttttcaatga gttcaatgtt tgtatcgcca tctccaagaa acacaatgtt
     7321 accatgggct ccagagagtt gcctaacgat aggcaatcct actatatcac aatagaatgc
     7381 aagggaacgt tccagatctt ttacgataat tgtagtccaa caatatctca ttcttttagt
     7441 cctcccattc caaggatact cggtgctccc acagtagggc aaccatgata catttgaaag
     7501 aggaaagaat ccggctagat tgcatcgcaa gaatctaggc taggccgtac tctcgacact
     7561 ctacctcaag atgggaatca ggatttttct caacaaccgc aggctctctg caaattttgg
     7621 tgcgaacctc aaggctatct ttaagagact catccttggt agaatacctt tttcgattct
     7681 aggcatggct agtgggagaa taggggtata ttctctgaga atactgacta ttcgaatgaa
     7741 aatgtgcacc gcgcactcga ttatttcctc gaatagaatc cagcgctcct tcagcatcta
     7801 cacgaagctc tgacagctca ttataagaga gatacggcct actatagtat gatttgacga
     7861 actactgctt tgagattcga gtgtctcttt ttgcttcatg aagtgcgaat cgatgcatct
     7921 tgataagctc aatggagact attcccaatg atctttttag cagcaaattg cgaaaaagtc
     7981 tggacaaaat ccagcctgta ctcctctcta acgaaaatca catcaaaact tatttcctca
     8041 tctgctttat atctttaatg ctcatgtgcc ttaaggtgac tttcgttatt ccgatttctg
     8101 gaatgatctc aacgccaata gttcttgttt ttcctattaa aagtttatca tctcattcag
     8161 catttgatgg tactcaatag cgcaaaaatg aatcagagtc caatgccaat ccataatcca
     8221 tatatgatcc ccatgttatg aaaaactgtg atttagcaat tctctttgtc tttcattccc
     8281 attgacagtc aaattcggat tatgcgttat cttatgattc cttatgggcg ctgacggatt
     8341 tgaaccgccg acctactggg tgtaaa
//
LOCUS       DAOOWY010000082         8246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_8640_length_8246_cov_6.652912, whole genome shotgun sequence.
ACCESSION   DAOOWY010000082 DAOOWY010000000
VERSION     DAOOWY010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8640_length_8246_cov_6.652912"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2530
                     /locus_tag="PK105_06715"
     CDS             <1..2530
                     /locus_tag="PK105_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU61441.1"
                     /translation="KTSDVQINVKGWKAIHLPHDMTEAPFNSFDERAFLRFGCYAKKL
                     EKEDIEKIVQGLGEERIAANRKPMLFLRFEGVSVFCRVWVNGRLASSHKGPYTPFEVR
                     IDSFIPRSWQEVCSKEAIEKEIDAKTARPIWILVEVDSSEDSAVPPFGGVVDYLAYGG
                     IYRGVSLCASIGARLSELWTVPRPRGDALDGPWLIHIRAEIETLQSGSIDNCFLIARL
                     YQAGELINEGNIFLLDSIINKGENRFSTDFSMSVDRPRLWDIDSPTLYDLELALHSEI
                     GQELDYVHLRIGFRTAEFGPSGFYLNQRRIFLRGLDRHQEYPYIGYAMGPDGQKCDAK
                     ILKQELGCIIVRTSHYPQSPHFLDACDELGLLVFEELPGWQHIGDSKWQEQALRNLED
                     MILRDRNHPSIVLWGVRINESKDNHEFYAKTNELARRLDPYRQTGGVRCHIRSELLED
                     VYTFNDFTYDGKGTMYISEPARVLPKYKKRAPYLITEHTGHMFPTKRFDQEERLVEHA
                     LRHAHVLDNAMGNSRVAGCIGWCAFDYHTHKDFGSGDRICYHGAADAFRIPKYAGFFY
                     GSQVSPSERLVLEPASIFAKGERNASHLLPIYIFTNCDAVDVYRSESFIARFFPDKAH
                     FANLPHPPIVIDDLIGSLIETEAWPQRDFRLFRKLAGKAMALGENGFDIWDKLRMALF
                     MRRQKLGIQDIEELVLRYGMNWGASDEKIRLVGILDGKEVVERSFGADSAAKRLSIEP
                     DALWLKSLDEEEWPSTRIVVKALDQYDNIAPFLFEPYSIDIKGPARLIGPARRSLISG
                     VSAFWISGKAKKGKVSIAVACPRFEEQAVVEFDIDRRSPSSISN"
     gene            2680..3459
                     /locus_tag="PK105_06720"
     CDS             2680..3459
                     /locus_tag="PK105_06720"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOU61442.1"
                     /translation="MGKKAISENESTYTDTDARQILEALFLIEDFFRYGVRCEHEISL
                     EASASAKPSSENRKATLEALKAQVDQCTACGLWQGRSQIVFGEGVLNPSVMVIGEGPG
                     IEEDRQGLPFVGASGQLLDKMLVAIDLSRHSNCYIANIVKCRPPNNREPSPSECDACI
                     GYLRAQISILQPDYILCVGRIAAHSLLRLSEPISKLRGRDFDFQGIPVRVTFHPSALL
                     RDTGLKRPAWEDLKQFRTLMESGVTKQRSAGRATQFSRPEN"
     gene            3463..5577
                     /gene="priA"
                     /locus_tag="PK105_06725"
     CDS             3463..5577
                     /gene="priA"
                     /locus_tag="PK105_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOU61443.1"
                     /translation="MPMSFVRIAYPIPLQETFWYRNDTSTKAEIGMRVEAPLGRRAKA
                     IGYVVEVIDIPASKSETVSRLESEDISPVKAVEPPLTKLGKPDLSADLEKPTLPPDLA
                     KLEPGKIRSINRVIDTEPLFDSEMLKLGLWMASMYHSSLGEALSAMLPSARRERSEIA
                     EQFDEIELSRTPLVLTQGQKAALDGILAEPKGMFYLYGPTGTGKTEVFLQLADAALSI
                     DLGVLYLVPEIALTRQVEQDARARFGERCAIIHSRLTPSRKLAEWRRIGKKEARIVIG
                     ARSAVFAPISDLGLIIIDEEHDASYKSGTTPRYHARQVAMRRARLEGARLVMGSATPS
                     PEAWQACREGQMLRFDLSNRPAGGSFPEMHIIDMRGRHGLISDELADALRDAKKAGRQ
                     SILFLNRRGFAHTLMCQTCGSQLLCKHCSVPLTYHKASNRLNCHYCGYHEIKPRACPS
                     CGSLDLAWASYGTERIEEELHERFPDMSHARLDTDTTNTKGYLEDTLLAFRNGKIDIL
                     VGTQMVAKGLNFPGVRVVGILMADQGLAMPDFRAGERVFSLIVQVAGRAGRHVPDGLV
                     FIQTRKPDSPIIQLASTGNVSGFLDRELQMRQQLEFPPATRLCRFVFRSKSAKSARSH
                     ASKCATLAHRLVCMSAFEGIDILGPSECPLAIVADNHRYQLIFRSDRFADLQRFVQTL
                     QHNIECTSSVYIEIDIDPVQMM"
     gene            5852..7189
                     /locus_tag="PK105_06730"
     CDS             5852..7189
                     /locus_tag="PK105_06730"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOU61444.1"
                     /translation="MVILTLNCGSSSVKYQVYDWEKKDVLANGIVERVTIGGSFITHK
                     VRNRPDYEVEHECPNHVVAIELILKTLVDPEVGVISDLSKIKAVGHRMVHGGSKFAKS
                     SIINGDFLETFESLNELAPLHNPANLMGVEAATTILPNVPQCAIMDTAWHQTMPAQSY
                     MYALPREWYEKYMVRRYGFHGTSFLYNAKRAAVLLGKDPFETNLIIAHIGNGASINAV
                     KNGCSYDTSMGLTPLEGLVMGTRSGDVDPGIIFHIIRKTGMTAAEIEKKLNKESGILG
                     LTTKWADRRDVANAAEKGDPSAILAQQVEAYRIKKYIGSYYAALGHVDAIVFTAGVGE
                     MSPVIRNLAVQGLQEMGIVIDQKKNALAMCRNAEFDITGLGSKVKIFIIPTDEELVMT
                     EDTVALINGTYDVHTNYHYYFENHDYVNRARAEGLQRDLEKKPWLKDIVARIP"
     gene            7554..>8246
                     /locus_tag="PK105_06735"
     CDS             7554..>8246
                     /locus_tag="PK105_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61445.1"
                     /translation="MKKLIVLLLSIAMVGAVFAQDAPAPVLKWSGLLNTGFQYDTSTD
                     SLYLYGQNAGKASRLRLNADYTNGDFGVHFRLQTNDAMTTQFTIAHALVWGNLFNNMA
                     TFKAGLLNDGTWATPYNSYGSLDGAKGVQLQVKPIEGLNVGAFMPLDTTKTVPAGNVP
                     KDMTFAGKYSISGIADVVAGLNLGVAKNEVYGSVNVTAVDNLTAIVEVDTSDISDIAN
                     TLVFDEYASYAMG"
BASE COUNT         2232 a         1911 c         2031 g         2072 t
ORIGIN      
        1 caagacaagc gatgtgcaga taaatgttaa aggctggaaa gcgatacatc tgccccatga
       61 catgaccgaa gcccctttta actctttcga cgaaagagcc tttttgcgct ttggatgcta
      121 tgctaaaaag ctcgaaaagg aagatattga gaagatcgtt cagggcttag gagaggagag
      181 aatagccgcc aatagaaaac caatgctatt cctgcgcttc gaaggcgtaa gtgtattttg
      241 tcgtgtgtgg gtcaatggca gattagctag tagccacaaa gggccctata cgcctttcga
      301 ggtacgcatc gattcattca taccgcgcag ctggcaggaa gtctgctcga aagaggctat
      361 agagaaggag atagacgcca agacagcaag gccaatttgg attcttgttg aagtcgattc
      421 ctctgaagat agtgctgtgc cacccttcgg tggagtagtc gattatcttg cttatggtgg
      481 tatctataga ggagtcagtc tatgtgcaag cataggagct agacttagtg agttgtggac
      541 agtcccgcgc ccaagaggtg atgccctgga tggaccgtgg ctaatacaca tacgcgcaga
      601 aatcgaaacc cttcaatcag ggtctattga taattgtttc ttaattgccc gcctttacca
      661 agctggagaa ctcataaatg agggaaatat atttctcctg gattccataa taaataaagg
      721 ggaaaacagg ttttcgacag atttctccat gagtgtagat agacccagac tctgggatat
      781 agacagcccc actctctacg atctggagct ggctctacat agcgagatag gccaagagct
      841 agattatgtg catctgcgca ttggttttcg gactgcagaa tttgggcctt cgggattcta
      901 cctgaaccag cggaggatat ttctgcgtgg gctcgatcgt caccaggaat atccatatat
      961 tggatacgca atgggtccgg atggccaaaa gtgcgatgcc aagatactaa aacaggaact
     1021 agggtgtata atcgtgcgta caagtcatta tcctcaatca ccccactttc tggatgcttg
     1081 tgatgagctt ggccttttag tcttcgaaga gcttccgggt tggcagcata taggagatag
     1141 caaatggcag gaacaagctc tgagaaacct tgaagatatg atattgcgcg atcgaaatca
     1201 tccgagtatc gttctatggg gagtacgcat caacgaatcc aaggacaatc acgagttcta
     1261 tgccaaaacc aacgagctcg ctcgaaggct ggatccatat cgccagacgg gaggtgttag
     1321 atgtcacatc cgcagcgaac ttctggaaga tgtctatacc ttcaacgact tcacctacga
     1381 tggtaaagga acgatgtata tctcggagcc cgcgagagtg cttccgaaat acaagaagcg
     1441 ggcaccctat cttataaccg aacatacagg acacatgttc ccaacgaagc gtttcgatca
     1501 ggaagagcga ctggtagaac atgcgctgcg ccatgcgcat gttctagaca atgctatggg
     1561 caattcacgc gttgcgggat gcatcggctg gtgcgctttt gactatcata cgcataaaga
     1621 ctttggttcc ggagatagga tctgttacca cggagcagcc gacgccttcc gcattccaaa
     1681 atatgccggc ttcttctatg gtagccaggt ctcgccctca gagcgcctcg tattagagcc
     1741 ggcttcaata tttgccaagg gggagaggaa tgcttctcac cttttgccta tctatatctt
     1801 tacaaattgc gatgccgtag atgtctatag atcagagagt tttattgctc gtttctttcc
     1861 cgataaggct cactttgcga accttccgca tccacccatt gttatcgatg atctaatagg
     1921 atcattgatc gaaactgaag cttggcctca aagagatttc cgccttttcc gaaaactagc
     1981 tggtaaggct atggccttag gcgagaatgg cttcgatatc tgggataagt tgagaatggc
     2041 gctttttatg cgacggcaga agctcggcat tcaagacatt gaagaacttg ttcttcgtta
     2101 tggcatgaat tggggggcat cggacgagaa aatacgcctt gtgggcattc tcgatggcaa
     2161 ggaagttgtc gagcgcagct ttggcgcaga cagcgctgca aaaaggctct ctatagagcc
     2221 agatgctcta tggctgaaaa gccttgatga agaggagtgg cccagcactc gcattgtggt
     2281 aaaagccctc gatcaatacg acaatattgc gccctttcta tttgaacctt actctatcga
     2341 tatcaagggg ccggctcgcc ttattggccc ggcgcggagg tctctaataa gtggagtcag
     2401 cgcattctgg atatctggta aggcaaaaaa gggaaaggtc agtatagccg tcgcttgtcc
     2461 ccgctttgag gagcaggcgg tcgttgagtt cgatatcgac aggcggtcgc cgagctcgat
     2521 atcgaattag aataataagt cctagtatcg aggaccttgc gattccagat gatgatagaa
     2581 gatgataggc gataataggc tataataggc gatgataaga gatgatgaga gatgataggc
     2641 gttcataaaa tttagattga atgagggttc gatttggcca tgggcaaaaa agctattagt
     2701 gaaaatgaaa gcacttacac agataccgac gctcgacaga ttttggaagc cctttttctc
     2761 atagaggatt ttttccgcta tggagtaagg tgcgagcatg agatatctct ggaagcttcc
     2821 gcctcggcca aaccatcgtc ggagaatcgc aaagcgactc tcgaagccct aaaagcgcag
     2881 gtcgatcaat gcaccgcttg tggcctttgg caaggtcgat cgcagatcgt attcggcgag
     2941 ggtgttctaa acccaagcgt aatggttata ggtgaaggac caggaataga agaagaccgg
     3001 cagggcttgc cctttgtagg ggcttcgggc caacttctcg acaagatgct tgtggcaata
     3061 gatctttcgc gacacagcaa ctgctatata gctaacatag taaaatgccg accacctaac
     3121 aatcgcgaac cttcgccttc tgaatgcgat gcctgcatag gctatctgcg agcccaaata
     3181 tcgatattgc agccagatta tattctctgt gttggccgca ttgctgcgca cagtcttctc
     3241 aggctttccg aaccgatttc caaattgcga ggccgcgact tcgatttcca gggaattcca
     3301 gtgcgtgtga cttttcatcc ctcagccctg cttcgtgata cgggtctaaa gcgacccgcc
     3361 tgggaggact tgaaacaatt tcgcacactc atggaaagcg gagtcacaaa acagagaagt
     3421 gcgggccgtg caactcagtt ttccaggccg gagaattagc gaatgcctat gtcctttgta
     3481 cgtatagcct acccaattcc gctccaagag actttttggt accgcaacga tacatcgacg
     3541 aaggccgaaa taggcatgcg cgtagaagct ccactcggaa gaagagccaa ggccatcggt
     3601 tatgtcgtgg aagtaatcga tatccctgct tccaagagtg agactgtctc acgtcttgag
     3661 tctgaggata tatcaccagt aaaggctgtc gagccaccac tgaccaaatt gggcaagccc
     3721 gacctgtcgg ccgatttaga aaagcccaca ctgcctcctg acttagccaa gctcgaacca
     3781 ggcaaaatcc gttctataaa tagggtaatc gataccgagc cactcttcga tagcgaaatg
     3841 ctgaagctag gtctatggat ggcctctatg tatcattctt ccctgggtga agccctctct
     3901 gcaatgctgc catcggctcg tcgcgaacga tcagagatag ccgaacaatt cgacgaaata
     3961 gaattaagca gaactccact tgtattaacc caagggcaaa aggctgctct ggatggcatt
     4021 cttgcggagc ccaagggaat gttctacctc tatggaccaa caggtaccgg caagacagaa
     4081 gtctttctcc agcttgccga cgcagctctc tcgattgatc taggtgtgct atatctcgtg
     4141 cccgagatag ctcttactcg ccaggtcgaa caggacgcgc gcgcccgatt cggagagcga
     4201 tgcgccataa ttcattctcg tttgacccct tcgcgcaaac tagccgaatg gcgcaggata
     4261 ggaaaaaaag aagcgcgcat tgtaatagga gcgcgcagcg ccgtctttgc tcccatctcc
     4321 gatcttggct taatcataat cgacgaagaa catgatgcca gctataaaag cggcacaact
     4381 cctcgatacc acgcaagaca ggtcgcaatg cgccgagcgc gccttgaagg agcacgcctt
     4441 gtaatgggca gcgcaactcc ttctccggag gcctggcaag cttgcagaga aggccagatg
     4501 cttcgctttg atctttcgaa tcgaccggct ggaggatcct tccccgaaat gcacataatc
     4561 gatatgcgag gtaggcatgg tcttatatcc gatgagctag cggatgcctt gcgcgatgcg
     4621 aaaaaagcag gccgccaatc cattcttttt ttgaatcgtc gaggatttgc gcataccttg
     4681 atgtgccaga cctgtggttc gcaattgctc tgcaagcatt gttccgtgcc tctaacctac
     4741 cacaaggctt cgaatcgtct taactgccat tattgcggct accatgagat aaaacctaga
     4801 gcatgtccat cttgcggttc gctcgatcta gcatgggcga gctacggaac agaacgcatc
     4861 gaagaagagt tgcatgagcg atttccggac atgagccatg cgcgcctcga taccgatacc
     4921 acgaatacca aaggctatct ggaagatacg ctgctcgctt ttcgcaacgg caaaatagac
     4981 atccttgttg gcacgcagat ggtcgcaaaa ggcctgaatt tccctggtgt gcgtgtagta
     5041 ggcatcctca tggccgacca agggcttgca atgcccgatt ttcgcgcggg agagcgcgta
     5101 ttttctctga ttgtccaggt agccggaaga gcgggaaggc atgtccccga tggacttgtc
     5161 tttatccaga caaggaaacc cgatagtccg attatccagc tagcctcgac gggcaatgtt
     5221 tctggttttc tcgatcgaga gcttcaaatg cgtcaacagc tcgaatttcc acctgcaacg
     5281 cgcttatgtc gttttgtatt tcgatctaag tccgctaaga gcgctcgaag ccacgcctcc
     5341 aagtgtgcca ccttagccca tcgccttgta tgcatgtcgg catttgaagg catagatatt
     5401 ctcggtcctt ccgagtgtcc tcttgccata gttgcggaca atcataggta ccagctcata
     5461 ttcagatcag atcgttttgc tgaccttcag cgctttgtac agacactgca acacaatatc
     5521 gaatgcactt cttctgtcta tatcgaaatc gatatcgatc cagtgcagat gatgtgataa
     5581 ttcaatttca atagctttgc catagacttg aggcctgggt taggctaatc ctgccgcctt
     5641 ttttcttggg tgctcgacaa tgctgtgcca aaataggtag ttactgtcgg gtgtacgatg
     5701 attttatgca aaggtagcca tgtggcattt tttcgagccc aataatcccc atcgcaataa
     5761 tcctcatcgc ggtgaacagg gtttaatttg ttgcatagtc tgccggacgg tgttatacta
     5821 gccggaataa tatctcctca ggagttctaa aatggttatc ttaacgctca actgcggttc
     5881 atcttcggtt aagtatcagg tctatgattg ggagaaaaag gatgttctcg caaacggaat
     5941 cgtcgagcgt gtcacaattg gcggttcctt cataacccat aaggtccgga acaggccaga
     6001 ctatgaagtc gaacacgaat gtcccaatca tgtggtcgcc attgagctca ttctcaagac
     6061 tctcgtcgac ccagaagtcg gcgtcatttc cgatctttca aagatcaaag cggttggcca
     6121 tcgcatggtg catggtggct cgaaatttgc caaatcctca ataataaatg gagatttcct
     6181 cgaaaccttc gaatctctca atgaactagc gcctcttcat aatccggcta acctcatggg
     6241 tgtagaagcg gccacgacca tactccccaa tgttccacaa tgcgcaatca tggatacagc
     6301 ctggcaccaa accatgccag cccaaagcta tatgtatgct cttccgcgcg aatggtacga
     6361 gaaatacatg gttcgccgat atggattcca tggaacgagc tttctctaca acgctaaacg
     6421 cgccgcggtg ctgcttggca aagacccatt cgaaacgaat ctcataattg ctcatattgg
     6481 caacggcgca tcgatcaacg cggtcaagaa tggttgttct tacgatactt ctatgggatt
     6541 gaccccccta gagggccttg tgatgggcac tagaagcggc gacgtagacc cgggaatcat
     6601 atttcatata attagaaaaa ccggaatgac cgctgccgaa attgagaaaa aactcaacaa
     6661 agaatcgggc atactaggcc ttacgacaaa atgggctgac cgccgcgatg tggcaaatgc
     6721 agcagaaaaa ggcgatccct cggctatctt ggcacagcag gtagaagctt acagaataaa
     6781 aaaatatatt ggatcatact atgctgcgct tggccatgta gacgctattg tttttaccgc
     6841 gggagtggga gagatgagcc ctgtaattcg caatctcgca gttcaaggcc ttcaagagat
     6901 ggggatcgtt atagatcaga agaaaaatgc ccttgccatg tgccgcaacg cagaattcga
     6961 tatcacgggc ctgggctcga aagtcaagat cttcattatc ccaaccgatg aagaattggt
     7021 catgaccgaa gacacggttg cgcttatcaa tgggacttac gatgtccata ctaattatca
     7081 ctattatttc gagaaccacg attatgtaaa cagagcacga gccgaaggct tgcagcgcga
     7141 tctcgagaaa aagccctggc tcaaggatat agtggcgcgt attccataga aaacaggctt
     7201 aaaagagcag ggaaatagtc aatattggct tccattgctc cgataatagt gatataatgg
     7261 cttttaattt gaaaaggagg tgatacagat agttttttat agtgtatcat cccaatatcg
     7321 gggatgtctg tcagattcca aataatgagc atggttcaga tccttgacaa ttgctcatag
     7381 agcgtataat tgcgctatct ggcaaaatag aatcttaata tcttatgaaa aaacttgaat
     7441 aaaataagct tgacattaca aaaagtgccg atatatactt ctgaacgaac ttagagtggg
     7501 gataaccaag ccgttggcaa ggcccccaat ctacttggta aggaggatta gacatgaaga
     7561 agctaatagt tttgcttctc tcgattgcaa tggttggcgc ggtttttgca caggatgcac
     7621 cagcccctgt tctaaaatgg agtggtttgc tgaacactgg tttccagtat gatacttcaa
     7681 ctgatagcct gtatctctat ggacaaaacg caggtaaagc ttcaaggttg agactaaatg
     7741 ctgattatac taatggtgat tttggtgtgc atttccgtct tcaaaccaat gatgcaatga
     7801 ccacgcagtt taccattgct catgctctcg tatggggaaa ccttttcaac aacatggcta
     7861 cgtttaaagc tggtttgctc aacgatggca cttgggctac tccatataac agctatggta
     7921 gcttagatgg ggcaaaaggc gttcagctgc aagtgaaacc gatcgaagga ctcaatgttg
     7981 gtgcgtttat gccattagac acaactaaaa ctgttccggc aggcaatgta cccaaagata
     8041 tgacttttgc tggaaaatat tcgatctctg ggattgctga tgtagtggca ggattaaatc
     8101 ttggcgtagc aaaaaatgag gtatatggtt ctgtaaacgt cacagcagtt gataatttga
     8161 cagcaatagt agaagttgat actagtgata ttagcgatat agccaatacg ctcgtatttg
     8221 atgagtatgc atcttatgca atgggc
//
LOCUS       DAOOWY010000083         7984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_9037_length_7984_cov_11.689747, whole genome shotgun sequence.
ACCESSION   DAOOWY010000083 DAOOWY010000000
VERSION     DAOOWY010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9037_length_7984_cov_11.689747"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK105_06740"
     CDS             <1..180
                     /locus_tag="PK105_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61446.1"
                     /translation="ASRGRNLFTNFITTLLGYLGLFFNDITISRLSKHDKQEFFLKDA
                     SFISNLGYMSILNQS"
     gene            278..1534
                     /locus_tag="PK105_06745"
     CDS             278..1534
                     /locus_tag="PK105_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61447.1"
                     /translation="MSKVYSEFDFTRKNNRPENTFKLFLYACVVIFLIACSDNSQYLP
                     EGFAPFYPKFEAWENCDEGFALICVSSADGAQGFLEWRTEGVYGRIVETSGSRGTRSF
                     LIGDGGMNGLVLNARGKGDSLEISYEPTSQNAYIFQEGASQHRVTANRSKAIKDLKLL
                     AGLSEETQESGRKISMRLQYLVSRESLEEKAFNDFLRRGNSPYKMAEFRRQRLEASPI
                     AKYELPEDSGNLDSEPAKNVLKDDGSGQIRYWIPPIEFYEIQYPVYVGENIISVATQY
                     YLFNGGAHGSASTDFDIIDRRTGKRLGPSDIFVSDWRSTLGPKLKVELIRQSGFWQDS
                     AKSAKVGNDGNSPIEDLRDLGFFESDISPSDKIFLCKTGIGFEYDRYEIAPWYMEEFI
                     ILLSWDELRPFLNPALFADGELLGSI"
     gene            complement(1531..4710)
                     /gene="ileS"
                     /locus_tag="PK105_06750"
     CDS             complement(1531..4710)
                     /gene="ileS"
                     /locus_tag="PK105_06750"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOU61448.1"
                     /translation="MYKPVDPKVNFSQQEESIVQFWQDQNIFQKSVSQREGAPEFSFY
                     DGPPFATGLPHFGHFVPGTIKDIFPRYKTMRGYHVERRFGWDCHGLPVENLIEKELGL
                     NSKTDIERYGIAEFNEACRGSVLRYVKEWRQIVTRLGRWVDFDHDYKTMEPDYMESIW
                     WVVKSLWDKGLIYEGHYILPYCPRCSTVLSNHELSLGGYEDVSDPSITVRFKITGLPP
                     SLDDPSLADLANGSTYFVAWTTTPWTLVSNLALCLGPDIDYVCVIDGSERYILADSKL
                     GDYYKNPESLKLSWKKKGSELAGIRYEPLFPYFAFLTEKGAFVTVLGQHVTTEEGTGI
                     VHTAPGFGEEDYEVLRGSGIPTVCPVDEECRFTEEVPDYKGLFVKDADKSIMNRLKNE
                     GKLIKRDQIIHAYPHCWRCESPLIYRAISSWFVEIEPIKKSMLKANKSVYWVPAHIRD
                     GRFGNWLENARDWAISRNRYWGNPLPIWRCDECGKTICVGSRKELAELGGEVPEDLHK
                     HYLDKVTLPCTCGGAMHRIPEVLDCWFESGAMPYGQNHYPFENKQKFESIFPADFICE
                     GLDQTRGWFYTLTILAAALFDKPAFKACVVNGLVLAEDGKKMSKSLRNYTDPSAVMDK
                     FGADALRLFLMNSAVIKAEDLCYSDEGVRNVLKSVILPLWNAYSFFVTYANIDGFVAE
                     DFDCAEVENPLDIWILSVCEKMIADVTTGFEAFDLQAAIAPTVEFVDSLNNWYIRRSR
                     RRFWKSENDADKKNAYKTLYRVLRRLTLVMAPVIPFVTEAIYRNIKLQSEPESIHLSD
                     WPNAEAKYRNLDLERDMRIVRQAVTMGRSLRVANDLKIRQPLGSIQLISRIAEEREAL
                     QRMEDILREELNVKSVVIHEKEEDLVEYHAKANFRVLGKELGPYMKEAAAKIANLSST
                     EIARLLDGEQILIALSSGEKIALDSSRVDIIREEKQGLKVLNEGSLTVALDTNITSEL
                     LYEGYVRDLIRGIQNARKEKGLDVTDRIILEVSGDDELKVALDRFGQIVAEETLALRI
                     DWKDIDKAMINGFAVEAGDKAWMVNLRKA"
     gene            5001..5876
                     /locus_tag="PK105_06755"
     CDS             5001..5876
                     /locus_tag="PK105_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOU61449.1"
                     /translation="MRTEGIDIDFRFIEDIQDVEGIRRIVIRVSEGNIIVTGSEDSQL
                     RYFIELQGVSEEIDTWSCRFRRAESIAIIEVIANDYVRIGKCNIFIPSHISDVEVHSS
                     RGSMEIREIPANVLAISDRGDISISNAKFVEVSSVNGNIIVDRSEGCSARSINGTIRC
                     SRIAGSVQVESQNSLVLMDQVEKNVAAVCDQGKIIARRVKGRVRLISNKGDIELEVSG
                     AFGGGEVQTYSGDISIQLEYSSIEFRAETLSGRIEANFPTSASGIGPQRCTFRTGEGS
                     RRLYVKSILGDIEIS"
     gene            complement(5959..7908)
                     /locus_tag="PK105_06760"
     CDS             complement(5959..7908)
                     /locus_tag="PK105_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU61450.1"
                     /translation="MANTIPQMYIEIAQRQPNVPVQLSKDAHGDFQPVTYGELLREVS
                     IFALGLDDFGVRRGDNIGIISDNRKEWLIADLAILGIGAVDVPRGCDATAQEIAYILS
                     WSGCSTTILENERQLKKILDVKKGCLASNSMSLIETPYADLRSIILIDPPSSQIAQEA
                     QEAGLNIHLFSEIMEKGLSLYRNDPDFYQRETLKGTRADLATIIYTSGTTGEPKGVML
                     SHGNFLHQTEYFPYLLEVNSGDVFLSVLPIWHSFERAVQYILLQAGCTIAYSKPIGSI
                     LMEDMEVVQPHWFSTVPRIWESVKDGVDKTIRQSGGFSKFMFSMFVGIGKAHAYFKNM
                     LYDQLPKFSPRSRFLDISISIIPFALLSPFRALGNALVFKKIKGKLGKRFIAGVSGGG
                     ALPPSVDRFFEAIGILILEGYGLTETAPVLSVRLRNHPVTGTVGPVHRGTEVRILDER
                     GNVLPAGQKGVIHVRGPQVMLGYYKRPDLTEKVLSKDGWLNTGDLGMFTIHGELKIIG
                     RVKDTIVLRGGENIEPLPIEQRLRESPYIQQAAVVGQDQRYLAALIVPNQEVLTAWAE
                     ENDIPFNDYPSLLEQPEVKELINSEINSYISIKNGFKPFERIYYFALLPSPFEEGREL
                     SAKLDLKRHVINELYADQIKKLFEE"
BASE COUNT         2109 a         1740 c         1871 g         2264 t
ORIGIN      
        1 gccagcagag ggagaaacct tttcacaaat ttcattacta ccctcctcgg atatctaggt
       61 cttttcttta atgatataac catatcaaga ttgagtaaac atgataaaca agaattcttc
      121 ttaaaagatg cctccttcat atctaatcta ggttatatga gtatattaaa tcaatcttaa
      181 ttatatcact tcccggcttt catagcaggg atatttgata taaaatttgt aagaatctct
      241 tctatacaat ataatttcgg cagggattgc agattatatg agtaaagtct atagcgaatt
      301 cgattttacc cgcaagaata ataggccaga gaacactttc aagctctttt tatatgcctg
      361 tgttgtcatt tttctgatag cttgcagcga taattctcaa tacttgccgg agggctttgc
      421 cccattctat ccaaaattcg aggcatggga aaattgcgac gaaggtttcg cattgatctg
      481 tgtttcgagc gcggatgggg cgcagggttt tcttgaatgg aggacggaag gtgtttacgg
      541 acgtattgtg gagaccagtg ggagcagggg tactcgttct ttcttgattg gagatggagg
      601 aatgaatggt ttggtattga atgccagggg aaaaggagac tcgcttgaga tctcctatga
      661 acctacgtct caaaacgctt atatatttca ggaaggagct agtcaacaca gagttaccgc
      721 gaatcgatca aaagccatta aggatcttaa attgctcgct ggtctgtccg aagagactca
      781 agaaagcggt cgtaagattt ccatgcgtct acaatatctc gtttcgcggg aatcgcttga
      841 agaaaaggca ttcaatgatt ttctccgccg agggaattct ccttataaaa tggctgaatt
      901 cagaagacag cggctagagg cttctcctat tgccaaatat gaattacccg aagattctgg
      961 aaatttggat tctgagccag cgaaaaatgt ccttaaggat gacggttcag ggcagattcg
     1021 atattggatt cctccgatag aattctatga gattcagtat cctgtatatg tcggtgagaa
     1081 tataattagt gttgcgacgc agtattattt attcaatgga ggagcacacg gttctgcttc
     1141 gacagatttt gatataatag atcgaaggac tggcaaaaga cttggaccca gtgatatttt
     1201 cgtatccgac tggagatcga ctcttgggcc aaagctaaag gtggagttga tcaggcaaag
     1261 cggcttttgg caggattcgg caaagtctgc caaggttggg aatgatggaa atagccccat
     1321 agaagatctt cgcgatctag gttttttcga atctgatata agcccatccg acaagatatt
     1381 tctgtgcaag acaggaattg gatttgaata cgatcgctac gagatagctc cctggtatat
     1441 ggaagagttc atcatcctgc tttcatggga tgaacttagg ccttttctta atccagcact
     1501 ttttgccgat ggagagctat tgggctcgat ctaggctttt ctcaggttca ccatccaagc
     1561 cttatcgccc gcttctactg caaagccatt tatcatggct ttgtctatat ccttccaatc
     1621 tattcttagg gctaaggttt cttcagctac tatttgacca aagcgatcga gtgcaacctt
     1681 tagttcatca tcgcccgaaa cctctagaat aatcctatcg gtcacatcga ggcctttttc
     1741 tttgcgggca ttctggattc ctcggatcag atcgcgcaca taaccttcgt agagcaattc
     1801 tgaggtgata ttcgtatcga gcgccacggt aaggcttcct tcgtttagga cctttaggcc
     1861 ttgcttttcc tcgcggatta tatctactcg ggagctatcc agagctattt tttcgcccga
     1921 gctcaaggcg attaatatct gctccccatc gaggaggcga gcaatctctg tgctgctcag
     1981 attggcgatc tttgctgccg cttctttcat ataagggcct agctccttgc ctagcacccg
     2041 gaaattggct ttagcgtggt attcgacgag atcttcttct ttttcatgga tgacaacgga
     2101 tttgacattg agttcttcgc gaagaatatc ttccattcgc tgcagagctt ctcgttcttc
     2161 cgcaatcctg ctgatgagtt ggatcgatcc gagaggctga cggattttga gatcgttggc
     2221 tactctgagc gagcgcccca tagtaacggc ttgtcgaacg atgcgcatat cgcgctccag
     2281 atcgaggttt cgatattttg cttctgcatt tggccaatcc gagagatgga tagattcggg
     2341 ctccgactgc agttttatgt tgcgatagat tgcctcggtg acaaatggaa ttacgggcgc
     2401 cataactagg gtgagtcggc gcagaacccg gtagagcgtc ttatatgcat tctttttatc
     2461 tgcatcgttt tctgatttcc agaatctacg ccgcgaacgg cgaatatacc aattgttaag
     2521 gctatccacg aattcgacag ttggagctat tgctgcttgt agatcaaagg cctcgaagcc
     2581 ggttgttaca tcggctatca ttttctcgca tacagatagt atccatatgt caagaggatt
     2641 ttcgacttcc gcgcagtcga aatcttccgc cacaaagcca tcgatatttg cgtatgtgac
     2701 aaagaaacta taagcgttcc aaagtgggag tattacgctc ttaaggacgt ttctgacacc
     2761 ttcgtccgaa taacagaggt cttcggcctt tattacagcg gaattcataa gaaagagtct
     2821 cagtgcatcg gcaccaaatt tgtccatgac tgcggagggg tcggtataat ttcgtaggct
     2881 ttttgacatc tttttgccat cttctgccaa gacaagacca ttgacgacac aagccttaaa
     2941 agcaggcttg tcaaaaagag ctgccgcaag gattgtcaat gtatagaacc agcctcgcgt
     3001 ttggtcgaga ccctcgcaga tgaagtccgc agggaagatg gattcgaatt tttgcttgtt
     3061 ttcgaaggga taatgattct gtccatatgg catcgctccg gattcgaacc agcaatcgag
     3121 gacttcagga attcgatgca tggctccacc gcaggtgcat ggcagtgtaa ctttatcgag
     3181 ataatgcttg tgcaggtctt caggtacttc gccgccgagt tccgcaagtt ctttgcgaga
     3241 acccacgcat atggtctttc cgcattcatc gcatctccag atggggagcg ggtttcccca
     3301 atagcgattt cgacttattg cccagtctcg cgcgttttct agccaatttc cgaatctacc
     3361 atcgcgaata tgggcaggca cccaatagac gctcttgttg gcctttagca tggatttttt
     3421 tataggctcg atctcaacga accagctgga aattgcccgg tagatgagcg ggctttcgca
     3481 gcgccagcaa tgtggatatg cgtggatgat ctggtcgcgc tttatgagtt taccttcgtt
     3541 tttgagccga ttcattatgg atttgtctgc gtctttgacg aataatcctt tgtagtccgg
     3601 aacttcctcg gtaaatctgc attcttcgtc gaccgggcat acggtaggaa taccggaacc
     3661 gcgcaatact tcgtagtcct cttcaccaaa acccggtgct gtatgaacta ttccggttcc
     3721 ttcttcggta gtaacgtgct ggccaagcac cgtgacaaat gcgccttttt cggtcaagaa
     3781 ggcgaaataa gggaataaag gttcgtatcg aattccagct agttcgctgc ctttcttctt
     3841 ccatgaaagc ttgagcgatt caggattctt ataatagtct ccaagtttcg agtcagccag
     3901 gatatatcgc tcactgccat cgattacaca gacataatcg atatctgggc caagacaaag
     3961 agctaaatta gaaaccagtg tccacggggt agttgtccat gcgacgaaat atgtagatcc
     4021 gttcgccagg tctgcgagag aaggatcatc gagcgatggc ggtagacccg ttattttaaa
     4081 cctcactgta atggaggggt cactgacatc ctcgtatccg cctagtgaaa gctcatggtt
     4141 ggataatacc gtagaacagc gcggacaata tgggagaata taatggcctt catatatgag
     4201 tcccttgtcc caaagggatt tgacaaccca ccaaatcgac tccatataat caggttccat
     4261 agttttatag tcatgatcaa aatcgaccca tcggccgagg cgagttacga tctggcgcca
     4321 ttcttttaca taccgaagga cagagcctcg gcaggcctca ttgaattcgg caatgccata
     4381 gcgctcaatg tcggtctttg aattcagacc aagttctttt tcgatcaggt tctctactgg
     4441 taatccgtga cagtcccatc caaagcgcct ttctacatga tagcctcgca ttgttttgta
     4501 tctaggaaaa atatctttga tcgtacctgg aacaaagtgg ccaaagtgag ggagtccggt
     4561 agcaaagggt gggccatcat agaaactgaa ttccggtgcg ccttcacgct gcgaaactga
     4621 cttctggaat atattctgat cttgccaaaa ttgaacgatc gattcttcct gttgagagaa
     4681 gttgaccttg ggatcaacgg gtttgtacac gatatcctcc tcggtaatgt cacgaataat
     4741 gctgtatgtt gcaaatcctt tcaagaggag gaagatagat gcaaagacaa aggaaatcaa
     4801 aaatggtaag gaatcgctaa aaggatcgcg aaatcgaaaa ggcctctagt gttggagtca
     4861 cgagatggga attcgtacac tattccaata tcgatttgct aaaaagagag attgtgatat
     4921 aataataaaa ataatattaa gatctgcatc ttacagaatg gcaggtaatg catggattga
     4981 gaacccggag gcataatttg atgcgcaccg aaggtataga tattgatttc cggtttatag
     5041 aagacattca agatgtggaa ggcatccgac ggatagttat ccgtgtgtca gaaggcaata
     5101 tcattgtaac cggatcagaa gactcgcagc tacgatattt tatagaactt caaggggttt
     5161 cggaagagat cgacacatgg tcttgccggt ttcggcgagc cgagagtatt gcgattatcg
     5221 aagtaatagc taatgactat gttagaattg ggaaatgcaa tatttttata ccttcacata
     5281 tctcagatgt ggaagtgcat tcctccagag gatcgatgga gattcgcgaa atcccggcaa
     5341 atgtccttgc catctccgat cgaggagata tcagcatctc gaatgccaaa tttgtcgaag
     5401 tctcgagcgt caatggcaat atcatcgtag atcgcagcga aggatgttcc gcccggagca
     5461 taaacggtac tatcagatgc tcgagaattg caggttcggt ccaggtggag agtcagaata
     5521 gtcttgtctt gatggatcag gtcgagaaga acgtagcggc ggtatgcgat caaggcaaaa
     5581 taatcgcccg cagagtcaaa ggaagagtca ggctcatctc taataagggc gatatcgaac
     5641 tggaagtcag cggtgcattt ggcggaggag aagtccagac ttattcgggt gacatttcca
     5701 tacagcttga gtatagcagc attgaattca gggctgaaac gctttcgggt cgaatagaag
     5761 caaacttccc cactagtgct tctggaatag gacctcagcg ctgtaccttt cgcacaggag
     5821 aagggtccag acgtctctat gtcaagagta ttctcggcga catcgagatt tcttgagctt
     5881 cttattcttt gggctatcat cggcattatt gacacgaaat ttctgattgg gttgaccagg
     5941 tttctcgtcg atataagatc attcctcgaa gagttttttt atctgatcag catagagctc
     6001 gttgattaca tggcgcttta gatccaattt tgccgacaat tctcgtcctt cctcaaaagg
     6061 agaaggcaga agagcgaagt agtaaatacg ctcaaaaggc ttaaaaccat tctttatgct
     6121 gatatagctg tttatttcgc tgttaatgag ttcttttact tcgggttgtt ccaataacga
     6181 tggataatcg ttaaacggga tatcgttctc ctccgcccat gcggtaagca cctcttgatt
     6241 tggtacaatg agagctgcga gatatctctg gtcttggccc actacagcgg cttgctgaat
     6301 ataggggctc tcgcgcaatc tctgttctat gggtaaaggc tcgatatttt ctcccccgcg
     6361 caatactatc gtgtctttta cacgacctat tatctttagc tcaccatgaa tagtgaacat
     6421 gcctagatcg ccagtgttca accagccatc cttagagagt actttttcgg tcagatcggg
     6481 tcttttgtaa tacccaagca taacttgagg accacgcaca tgaataacgc ccttttgccc
     6541 tgcgggaagc acatttcccc gttcgtctaa aatgcgaact tctgttcctc gatgtactgg
     6601 acctaccgtg ccggtcaccg gatgatttct caacctcacg ctcaataccg gcgcggtttc
     6661 tgttaggcca tatccttcga gtattagtat tccgatggct tcgaagaagc gatcgaccga
     6721 gggcggcaag gctcctccgc cagatacgcc tgcaataaaa cgctttccaa gtttaccctt
     6781 gatttttttg aacacgagcg cattgcccaa ggcgcgaaaa ggactaagca atgcaaaggg
     6841 aattatagat atggaaatat caagaaagcg cgacctggga gagaattttg gcagttggtc
     6901 gtacagcata ttcttgaaat atgcatgggc ttttccaatg cctacaaaca tagagaacat
     6961 gaatttggag aatcccccgc tctggcgaat tgtcttatcc accccatctt tcacggattc
     7021 ccagatccga ggaacggttg aaaaccagtg gggttgtact acctccatat cttccatgag
     7081 aatcgagcct ataggcttgg aataggcgat cgtacatccg gcctgcagga gaatgtactg
     7141 aacagctcgt tcaaatgaat gccaaattgg aagtactgaa aggaacacat cgccactatt
     7201 cacttcgagc agatagggga agtattcggt ttgatggaga aaattgccat gactgagcat
     7261 gacacctttt ggttcgccag ttgtaccaga agtgtagatt atggttgcaa gatcggctct
     7321 tgtacctttt agagtctctc tttgatagaa atcaggatca tttctataga gtgataggcc
     7381 tttttccata atttccgaga atagatgaat attgagccct gcctcttggg cttcctgggc
     7441 gatttgtgac gatggaggat cgatcaaaat tatggacctt aaatcagcat aaggtgtttc
     7501 tatgagagac attgagttgg aagcaaggca gccttttttg acatcgagga ttttctttag
     7561 ttggcgttca ttttcgagaa ttgtagttga acatccactc cagcttagaa tatatgcaat
     7621 ttcctgggct gttgcgtcgc agccgcgtgg aacatcgacc gctccaattc cgagaatagc
     7681 gagatccgca ataagccatt cttttctatt gtcggaaata atacctatat tgtctcctcg
     7741 cctgacacca aaatcatcca atccaagcgc aaagatgctg acttctctta ggagttcacc
     7801 ataggttact ggttgaaaat cgccatgcgc atccttggaa agctgtactg ggacatttgg
     7861 ctgtctttgt gcgatctcaa tatacatctg agggattgta tttgccattg ccaagagcct
     7921 cctttgacaa ttttgacatt attaccataa atgtaaaatt gaaaatggtc aaggtactgc
     7981 gttg
//
LOCUS       DAOOWY010000084         7920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_9129_length_7920_cov_6.513668, whole genome shotgun sequence.
ACCESSION   DAOOWY010000084 DAOOWY010000000
VERSION     DAOOWY010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9129_length_7920_cov_6.513668"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1186
                     /locus_tag="PK105_06765"
     CDS             62..1186
                     /locus_tag="PK105_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU61451.1"
                     /translation="MNTNIHRKSYCVVIGGLNLDIQAFCYSEYQPGDSNPGQIHRSPG
                     GVGRNIAENLLRLGLDVELMTMVGDSSSWDRLISRTEKLGISLAHSPRIKKAPLSVYL
                     CILGRDGDLVGAVADMRAMEKMQIHQLQARKFLLDSAEAIVVDGNIPQDCIEWIAMNY
                     GRLEYEDKQHISCINKGSRDSSASHRPLLIADPVSTKKAEKFISSFGKFDLAKPNINE
                     AAKIAGLEQNASIEAIALSLREKSLLPKELYISQGGQGMSFVDKEIIEHIPLSSSENC
                     PHVINRSGAGDAACAALAWISLLERRIGKNCKDSPLSISPRDKAKIALSSAIYASSSN
                     RPVNPHLDQRNLFETTRICYPETSELINELIDAIQMENHK"
     gene            1183..2085
                     /locus_tag="PK105_06770"
     CDS             1183..2085
                     /locus_tag="PK105_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOU61452.1"
                     /translation="MNKYLDISEEVTDAISVGMPVVALESTIITHGMPWPQNLETALK
                     VENAVREEGAVPATIAIINGKLKVGLTNKELVSLANGSLKPIKASRRDFPALIAFQGN
                     GSTTVAGTMIIAEMAAIHVFATGGIGGVHRGAEKSWDVSADLEELAHTDVAVVCAGAK
                     AILDLPKTMEYLETKGVPVIGYGTSELPAFYTQHSGVRLSLRADTPQEIAMILRAKWE
                     SGLKGGVVIANPIPSQYSMSETTIEMAIQKAIGEAEQKGLNGAEVTPYLLSRVADLTG
                     GDSLEANIALVLNNARLAAKIAKA"
     gene            2226..3005
                     /locus_tag="PK105_06775"
     CDS             2226..3005
                     /locus_tag="PK105_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOU61453.1"
                     /translation="MRIIRCLYEDTIYFGYIEDDLVILSKDLPRIEIHNHEKKVWFER
                     EVAKVPFAKVFLEAPCIPTKAVCVGLNYRDHAEEFGLKIPSSPVLFIKPSTSLLSPGK
                     PIHYPTLSKQVDYEAELVVVMGKSAKNVDAKHAFDYVLGYTCGNDVTARDLQPKDGQW
                     TVAKSFDTFMPLGPWIETDLDPSSLKLRTYLNDEPKQFSSTSNLIFDVPSLIEYISQI
                     MTLNPGDVIMTGTPSGVGPMQKGDHVCVEIEGIGRLENTVE"
     gene            3019..3933
                     /locus_tag="PK105_06780"
     CDS             3019..3933
                     /locus_tag="PK105_06780"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU61454.1"
                     /translation="MALSRILSIFLLIAVGYCARKAKLLDENGIRTVSNLVLTIALPC
                     TIISSFDRSIPFSAARDLIKVAIYAIAIHVISIAISEVLFSRMERTKKKILIYAAVFS
                     NCGFMGFPLAESIFGHSGLMYVSIYVMIFNIFTWSYGLALLSRGEKSDQRTKKIRIRD
                     MLFNPGIIAVAIGFVLWILPFSMPQTITYALALIANCTTPLSMIVVGATLASLGVKGL
                     FSGMEVWIGSLMRLLVLPTIFIILMKILRQDNFPVKVANLLVAMPAAAQTVIFAERHD
                     ADVLLASKIVFISTVLSAVTIPIVAQLL"
     gene            complement(4059..6125)
                     /locus_tag="PK105_06785"
     CDS             complement(4059..6125)
                     /locus_tag="PK105_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU61455.1"
                     /translation="MNSGKIMKDFRRRFKASNPWVWPIYLFLIVFVIFPLVRLFVDSF
                     STADIPLSSLNEVVLTTDLLEEMVSNGEMEGAKAVAHDIAIQLNRSLDTIDRMIQALS
                     RATFSQEELTAKWQDAKDRVAQEAAVMAELQDDPVAALAEAKEATVLIQEIVRLPRQA
                     FSVKFFLDVFKDKLYMKALANSLLLGLATVITTSIIGFTVAYLLVRFDFPGRNLFSFL
                     TTVPIVMPPLVGVLGFIFILGRGGTVNELLYQLAEGLEKSWPFMSNWLYNHLPFNFIY
                     GWHGLLLVETCHLFPFITLNVLDSLSKIDASLEEAAESVGSVGFRRLFDVTIPLTIPG
                     FITGALLVFIGAFADFAAPMVVGLTNFIAPLAYMDIQQFTDRHLFKMGITVGAVMVLL
                     SIVFLLIAKKYISLKDYSTLSYRAVERKPLKGKGAVLTLVFLILLMLICFIPYAGVAL
                     ASFARSWSMSPLPTSWTMVHYEKVLLYAPGYIINTFRFCILAIIMCILIGVPISWIMA
                     RTQMRTRGALDILTMLVLALPGTALGIAYIRAFNTPILLATPLANSWVILPIVLAVRR
                     LPYTVRSSYSSLLVVHHSMEEAAESVGAGGLKTFKDITLPLIWKGVLAGALFSFMTSI
                     QESAATLLLSIPGWETMTMGIFTFYQGGTHGDAAALGSILIVVGVITLFAVNRLSSVA
                     QGGLFG"
     gene            complement(6172..7239)
                     /locus_tag="PK105_06790"
     CDS             complement(6172..7239)
                     /locus_tag="PK105_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61456.1"
                     /translation="MDIKLNGVTKRFKSVEALSRVNLHIRDGELFTLLGPSGCGKTTT
                     LRLIAGFYIPDEGKIMFGDREVQEIPTSKRNIGMVFQNYALWPHMTVYKNIAYGLQFK
                     NVPKSEWPRIINENLIKVGLVGYEKRYPGQLSGGQQQRVALARALAINPDVLLLDEPL
                     SNLDAKIRESVRAEIRKLQQSLRITTVYVTHDQEEALTLSDRIGVMCEGKLIQIGTPH
                     DLYEKPATRFVADFIGTNNLVAGTVESVNEDIIVITELGTMRGFSYSTMKPGDKCTVA
                     FRPENVEVYTAQPSSQDNQIHGKIGFASYMGNTLRYEMEHPAGVVLKADIKNPLQHEE
                     LGWGSDVYFCFPKSAALVIPD"
     gene            complement(7367..>7920)
                     /locus_tag="PK105_06795"
     CDS             complement(7367..>7920)
                     /locus_tag="PK105_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU61457.1"
                     /translation="TYEVILQKYGWDKGWEWLSKLAAYTGQFVAKSGDVPAVVAKGEY
                     ALGFAVPSYMAFENFLHGGDVRFIAPPGAYVTPEPISIIKNCKNPNAAKAFIEFLLTD
                     EGQRLFIDRGLFPIVPELRVEGPPGSTAELAVTFYGGRRSYFEGVVENTYDNSLSQSR
                     EGEVNKYFRDNIFAKLDALKAKY"
BASE COUNT         2151 a         1853 c         1855 g         2061 t
ORIGIN      
        1 taaataagat ttctgatgat aatatagcat atttgcaaat cttggtggag ggcggaacac
       61 cttgaatact aatatacaca ggaagtccta ctgtgttgtt attggaggct tgaatctaga
      121 tattcaggct ttttgctact ctgaatatca gcctggtgac tcaaatcccg ggcaaattca
      181 taggagccca ggaggcgttg gccgcaatat agcagagaac ctattgcgac ttgggttgga
      241 tgtggagctg atgactatgg tcggagattc ttcaagctgg gataggctta tctctcgtac
      301 cgagaagttg ggcataagtc tcgctcacag cccgcgcatc aagaaggcac ctctatctgt
      361 ctatctgtgc attctaggaa gagatgggga ccttgtgggc gccgttgcag acatgcgtgc
      421 catggaaaaa atgcagattc atcagcttca agctcgaaaa tttctgctcg attccgcaga
      481 agcaattgtg gtggatggaa atattcctca ggattgtatc gaatggattg ccatgaatta
      541 tgggcgctta gagtatgaag ataaacagca tatatcttgc ataaataaag gatccagaga
      601 ttcaagtgct tctcatagac ctttactcat tgctgaccct gtttctacaa aaaaggcgga
      661 aaaattcatc tcgagctttg gaaaattcga cctggcaaag ccgaatataa atgaagctgc
      721 taaaatagcc ggcctggagc aaaatgcttc tatagaagcg atagctttgt ctctaagaga
      781 aaagtccctc ttgccaaaag agttgtacat ttctcaagga gggcagggca tgagttttgt
      841 agataaagag attatcgaac atattccatt gagttcctca gagaattgtc cgcatgtaat
      901 caatcgttca ggcgctggcg atgcagcctg cgctgccctg gcctggatct cattgcttga
      961 gagacgaata gggaaaaact gcaaagatag cccattgtcg atttctccca gagacaaggc
     1021 aaaaatagcg ctgagttctg caatctatgc cagctcttcg aatagaccag tcaatcctca
     1081 tctggatcag cgcaatctat tcgaaaccac aagaatctgc tatcctgaaa catcagagct
     1141 gataaatgag cttatcgatg caatccaaat ggagaatcat aaatgaacaa gtatcttgat
     1201 atctcagaag aagttactga tgcaattagt gtaggaatgc ctgtagtcgc attagaatcg
     1261 acgataatca cccacgggat gccctggccg cagaacctcg agactgcgct gaaggtagaa
     1321 aatgcggttc gagaagaagg agctgtccct gctactattg cgattattaa cggcaaacta
     1381 aaagtcggtc taacaaataa agagcttgtt tcgcttgcga atggttctct taagcccatc
     1441 aaagcatcac gaagggactt tccagcgctt atagcctttc aggggaatgg ctcaaccacg
     1501 gttgcgggaa ccatgataat cgctgaaatg gcggcaatcc atgtatttgc gactggtggc
     1561 atcggcggag tacatcgcgg cgcggagaaa tcctgggatg tctcagcgga cttagaggaa
     1621 ttagcccaca ccgacgtggc ggtggtttgt gcaggcgcca aggctatact agaccttcct
     1681 aagaccatgg aatacctaga aaccaaagga gtgccggtga tcggctatgg aacatcggag
     1741 cttcccgcat tctataccca gcattctggc gtcagactct cgctcagggc tgataccccc
     1801 caagagatag caatgatact tcgcgctaaa tgggaatctg gattgaaggg cggagttgtc
     1861 atcgcaaatc ctattccttc tcaatattcc atgagcgaaa ctacaataga gatggctatt
     1921 caaaaagcga taggagaagc cgagcaaaaa gggttgaatg gtgccgaagt tactccatat
     1981 ctgctttcaa gagttgcgga tttgacgggc ggcgattccc tcgaagcgaa cattgcactt
     2041 gtgctgaaca atgctcgtct agcagccaaa atagccaaag catagagcgc aaaacgactt
     2101 tgtacgactt tgtgcaaagc caatcacaaa gaatataaat aattgtattt gataaatgct
     2161 tatgatagga tgaatatcac ttcgtatcca ttaatgaggc ctatgcaacg ttttcggagt
     2221 aaaaaatgag aatcattaga tgcctttatg aagacaccat ctactttggc tacattgaag
     2281 atgatctagt cattctttcg aaggatcttc cgcggatcga gattcacaac catgaaaaga
     2341 aggtttggtt cgaaagagaa gtggccaagg ttccatttgc taaagtattt cttgaagccc
     2401 cttgcatccc gacaaaagcg gtatgcgttg gtctcaacta cagagatcat gccgaagagt
     2461 tcgggctcaa aattccatct tctccggttt tgttcattaa gccatcgacc tcgctcttga
     2521 gccccggaaa gcctatacac tacccaacat tgtcaaaaca ggtagattat gaggccgagc
     2581 ttgtggtagt tatgggaaaa tcggcaaaga acgttgatgc caaacatgcg tttgattacg
     2641 tgctaggtta tacatgcggc aatgacgtga ccgcacggga tctgcagcct aaagatggtc
     2701 aatggaccgt ggctaaatcc ttcgatacct ttatgccatt aggtccttgg atcgagaccg
     2761 acctagatcc ttcatctttg aaactaagaa cctatctcaa tgatgagcct aagcagtttt
     2821 catcgacctc aaacctgatt ttcgatgttc cttccttaat tgagtatatc tctcagatta
     2881 tgactctcaa tccgggagac gtgatcatga ctggcactcc ttccggagta ggccctatgc
     2941 aaaagggaga tcatgtatgc gtcgaaatag aaggtatcgg tcgacttgag aacactgtag
     3001 aataaaaagc gctcaattat ggccttgtcg cgaattctat ccatttttct cttgatagcg
     3061 gttgggtatt gcgctagaaa agcaaagctg ctcgatgaaa atggtattag aacagtctcc
     3121 aatcttgtac tcactatcgc tcttccttgc accataattt cgagtttcga tcggagtatt
     3181 ccgttttctg cagcaagaga cctaattaaa gtagcaatat atgctattgc gattcatgtt
     3241 atttctatag ctatttcgga agtgcttttt tcaaggatgg aaagaaccaa gaagaagatc
     3301 cttatctatg cggcagtgtt ctctaattgt ggattcatgg gttttcctct cgctgagagc
     3361 atttttggtc attctggtct tatgtatgtg tctatctatg tgatgatttt caatattttc
     3421 acatggagtt atggtcttgc actattgtct cgaggtgaga agagtgacca aagaactaag
     3481 aagattcgga taagagatat gcttttcaat ccaggaatca tagcggtagc tataggcttt
     3541 gttctttgga tcttgccttt ttcgatgcct cagacaatca cctatgccct agctcttatt
     3601 gccaactgta caacgccttt atccatgatt gttgtgggag ccacattggc cagtcttggt
     3661 gtgaaaggct tgttttctgg aatggaggta tggataggat ctttgatgag gcttctggtt
     3721 ttgccaacta tttttataat tcttatgaaa atactgagac aggacaattt tccagtaaaa
     3781 gtagccaacc ttttagtggc aatgcctgca gcggctcaaa ccgtcatctt cgcagaacgc
     3841 cacgatgcag atgtcttgct cgcatcaaaa atagtcttta tttctactgt actatccgcg
     3901 gttacaattc ctatagtggc gcaattgctc taagcatgat tgattgttag gtatataatg
     3961 gcataagtta taaaaactaa ggcgaaatcc gacaaatcta acttatccac ggtcgatttt
     4021 gcctttgctc aaaccctctt tggtcaactc ggcttacctt atccgaacag tccaccttgc
     4081 gcaacactac tgagtctgtt aactgcaaat agggtaatca cgcccacgac tataaggata
     4141 gagccaagcg cggctgcatc tccatgggtg cctccctggt aaaaggtaaa tatgcccatg
     4201 gtcatagtct cccaccctgg tatcgacaaa aggagtgtag ctgcgctctc ctgtattgac
     4261 gtcataaaag agaacagcgc tccagccagc actcctttcc agatcagagg aagagtgatg
     4321 tccttgaatg tcttgaggcc tcctgcgcca acagattcgg ccgcctcctc catagaatgg
     4381 tgcactacaa gcagagagct ataggacgat cgcacagtgt aaggaagtcg ccgcacggcc
     4441 agtactatgg ggagtatgac ccagctgttt gctagtggcg ttgctaagag aatcggagta
     4501 ttaaaagccc tgatataggc gatgcccaat gcagtgcccg gcagagccag caccagcatt
     4561 gtcaagatat ccagtgctcc acgagtgcgc atctgagtcc tcgccatgat ccacgatatt
     4621 ggcacgccta tcagaatgca cattattatc gctagtatgc agaatcggaa tgtgttgatt
     4681 atatagccag gtgcgtacag caagaccttc tcataatgga ccatagtcca tgatgtaggc
     4741 agcggcgaca tggaccagct tcgcgcaaac gaggctaacg caactcccgc atacggtata
     4801 aaacagatga gcattagtag aattagaaac accagagtca aaacagcccc tttacccttg
     4861 agcggttttc tttccacggc tctgtaggag agagtgctgt aatctttcag cgaaatgtac
     4921 ttcttggcta tcaacagaaa tacaattgaa agaagcacca ttacggcgcc cactgtgatt
     4981 cccattttaa acagatgcct atcggtgaac tgttggatat ccatgtaggc taatggagca
     5041 atgaagttgg tcagtccaac caccatagga gccgcaaagt ctgcgaaagc ccctatgaac
     5101 acaagaagcg ccccggtaat aaagcccggt atggtcaatg gaatagtcac atcaaagaga
     5161 cgcctaaagc ctacagaccc aacgctctct gcagcttcct ctagtgaggc atctattttg
     5221 ctgagcgagt cgaggacgtt cagcgtgatg aacgggaaca agtgacaggt ttccaccaac
     5281 agaagaccgt gccagccata tataaagtta aatggcaagt gattatatag ccagttggac
     5341 atgaaaggcc atgatttctc caggccttcc gcaagctggt ataggagctc gtttacggta
     5401 cctcctctgc cgagtatgaa gatgaagccc aacacaccta ctagaggagg catgactatg
     5461 ggaacggtag taaggaaact gaagagattt ctaccaggga agtcgaacct aacaagcagg
     5521 taagccacgg taaatccaat gatcgatgtt gttatcaccg tagcaagccc gagcaacagc
     5581 gaattcgcaa gggctttcat gtatagctta tctttgaata catccaggaa gaacttcacg
     5641 gaaaatgcct gacgaggcag cctaacgatc tcctgaatca gaaccgtagc ttccttggct
     5701 tctgctaggg cagccaccgg gtcgtcctgc agttcggcca ttactgctgc ttcctgcgct
     5761 acacggtcct tggcatcctg ccacttggct gttagctcct cctgagagaa cgtggctctg
     5821 ctgagagctt gtatcatgcg gtcaatcgta tctaaagacc gatttagctg gatcgctata
     5881 tcatgggcga ccgctttagc gccttccatc tcaccgttgc tcaccatctc ctctagcaaa
     5941 tcggtagtaa ggacaacttc gtttagcgac gatagcggta tgtctgctgt ggagaacgag
     6001 tcgacaaata atctcacgag ggggaatatg acgaacacaa tcaggaatag atatatcggc
     6061 catacccacg gattggaggc cttgaaccgc cttctgaaat ctttcatgat cttccctgaa
     6121 ttcattaaac aaccacctgc ttttgcatga tcaatgtcag catgtcagtc gtcagtccgg
     6181 aatgacaagc gccgcagact ttgggaaaca gaaatacaca tcactgcccc aaccgagttc
     6241 ctcatgttgt aagggattct tgatatcggc cttgagtaca accccagccg gatgctccat
     6301 ttcgtagcgc agcgtatttc ccatgtagct ggcaaaaccg attttgccgt gtatctgatt
     6361 gtcctgtgaa gacggctgcg cagtgtagac ttccacgttc tcaggccgaa atgccacagt
     6421 gcacttgtcg ccaggcttca ttgtagagta actgaagccc ctcattgtgc ccagctctgt
     6481 gatgactata atgtcctcgt tgacagattc caccgtgcca gccacaagat tgttggtgcc
     6541 gatgaaatca gccacaaagc gcgtcgctgg cttttcgtag aggtcatgtg gtgtacctat
     6601 ttggatcaac ttgccttcgc acattacgcc gatacggtca gagagtgtga gtgcttcttc
     6661 ctgatcgtgt gtaacgtaca cagtagttat tctcaatgac tgttgaagct ttcggatctc
     6721 tgcacgcact gattctcgga tcttggcatc cagattggag agtggttcgt ccaggagcag
     6781 cacatccgga ttgatcgcaa gagcccttgc gagcgccaca cgctgctgct gacctcccga
     6841 cagctgtcct ggatagcgct tttcgtaacc aacaagcccc accttgataa ggttttcgtt
     6901 gattatcctg ggccattcag atttgggcac attcttgaat tggagtccat atgctatatt
     6961 cttgtatact gtcatatggg gccatagagc gtagttttgg aacaccattc caatattacg
     7021 cttggatgtt gggatttcct ggacttcacg gtcgccgaac atgatcttgc cctcgtcggg
     7081 gatgtagaat ccggcgataa ggcgcagggt agttgtctta ccacagccag aaggtccgag
     7141 caaggtaaag agctcgccgt cccttatgtg cagattaact cgactcagcg cttcaacact
     7201 cttaaacctc ttcgtaaccc cattaagctt gatatccatg catttcacct caagttaagc
     7261 gactattccc aaagtcaaac gactactaaa gggagcccat ggacaaggca aactcgccca
     7321 tgggctatca gcaattcatg tattcaaaag aaatcaagca ccagaattag tacttcgcct
     7381 ttaaggcgtc cagttttgca aagatgttgt ctctgaagta cttgttgact tcgccttctc
     7441 tgctctgcga cagtgagtta tcgtaagtgt tctccacgac gccttcaaag tagctcctgc
     7501 gacccccata gaacgtgact gcaagctcgg ccgtagatcc gggagggcct tccaccctca
     7561 attcgggcac gattgggaac aggccacgat cgatgaacaa cctctgaccc tcatcggtga
     7621 gtagaaactc gatgaaagcc ttggcagcat tggggttctt gcagttcttg attatggata
     7681 tgggctcagg cgtaacgtag gctccaggag gtgctatgaa cctaacatct cctccatgca
     7741 ggaaattctc aaacgccatg tagcttggaa ctgcaaaacc cagcgcatat tcgcctttgg
     7801 ccacaactgc gggaacgtcg ccggatttag ctacgaactg ccctgtatag gcggcaagtt
     7861 tggacagcca ctcccagcct ttgtcccagc cgtatttttg cagtatgacc tcataggtag
//
LOCUS       DAOOWY010000085         7833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_9257_length_7833_cov_11.307791, whole genome shotgun sequence.
ACCESSION   DAOOWY010000085 DAOOWY010000000
VERSION     DAOOWY010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9257_length_7833_cov_11.307791"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1635)
                     /gene="murJ"
                     /locus_tag="PK105_06800"
     CDS             complement(64..1635)
                     /gene="murJ"
                     /locus_tag="PK105_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOU61458.1"
                     /translation="MVRNAGKLSLLTFISRVLGLIREMTKARFLGTGIYSDAFTVSFI
                     IPNFLRRLFAEGSVTVAFIPTYKGYLHAQNDSETREFLSASLTILSICVTIVVAIGIA
                     LAPIIVKLFGSDPMETAILTRIMFPFLAFVSFAALLQGILNAHEIFGPSGFAPILFNV
                     CFIVVPWILGDRLGNPARSMAVGVVLGGLAQALVQLPAVLRLSTHIGLMNPIRALRNS
                     GMRKVFALIAPTILGMAAYQVNDLVSTAYASRAGVGVASSLQYSLRLQELILGIFAVS
                     AGTVLLPRLADTVRQGDWAEYSKILGNTMRSLLLLTVPVAVFSMAFTVRIVTLLFKTG
                     DFSDVSVQLTASAFFWHQTGLVFIAMNRLIAPAFYARSNTKTPTFAGIASFAVNIILV
                     LLLAFRFQGAGIAFALSFSSAVNTVLLVWALIKDSIEGIRNELWLSLKYMSKMLIFSA
                     IAIVPAILVDKAVFKAVSQYGSRFVYAGLPLVTSTLVFAAIGIALLVLTKDTVAASIT
                     TALSRRSRGKNASQS"
     assembly_gap    1654..1753
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1996..2811)
                     /locus_tag="PK105_06805"
     CDS             complement(1996..2811)
                     /locus_tag="PK105_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOU61459.1"
                     /translation="MRALSFLATTFILAFILVVIVSCSGQKDNRLSSLPSVIRDQVEK
                     ALSVVQFSDTDRNRVRDAINNDPAGFAVLFSEVREIMKNDTNLLCRVDKQKALSAQYV
                     PMDLVDLDGPFPYIVSKKGMKLRKPAQEALVRMAEMAKKDGIALVVSSAYRSYDYQKI
                     VFEQNVNEMGRAEAERVSAVPGMSQHQLGTVVDFGSISDEFAGSDAERWLASNAGKFG
                     FSLSFPKDMENITGYKWESWHYRYISPPAVELQERFFLGIQQYLIEFLAALPN"
     gene            complement(2980..3759)
                     /locus_tag="PK105_06810"
     CDS             complement(2980..3759)
                     /locus_tag="PK105_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOU61460.1"
                     /translation="MKSDTQLKLAVLIDADNTQPTIIDGLLAEIAKYGVASVKRIYGD
                     WTNPTLRGWKERLLEYAIQPVQQFAYTTGKNSTDSAMIIDAMDLLYTENLDGFCLVSS
                     DSDFTRLAARLREDGKLVLGFGQRKTPKPFLAACDKFIYTEILQANVNETEEGRESSS
                     EKEGGKGSLPQADIKSDTKMKALLASAVDEAADEFGWAYLGEVGTYLANRLPEFDPRN
                     YGFRKLGELIKAVNLFEIDERTNPMVPGKQVYLRLKQRGKN"
     gene            complement(3780..5225)
                     /locus_tag="PK105_06815"
     CDS             complement(3780..5225)
                     /locus_tag="PK105_06815"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOU61461.1"
                     /translation="METTHVSESPGHSLRADAEEIFRAAVDRVMPGRLFSYCLSLEGS
                     VLRLWDGEEPQAYDLSKYDHVVIVAFGKASIPMTASLASILGQRVTEGLVVSKAPESG
                     QSSRLSEDIESLFASRSITSIESSHPVPDKRSVQAGSEMLNLALRVRKWEEAGERTLV
                     FVLVSGGGSALLAVPAPGISLEDKAEVTRLLLGCGANIHEINAVRKHLSSIKGGHLAK
                     AFFPSRIIALVLSDVMGDDLDTIASGPTVPDTSTWRDVKAIFERYALLDRLPKSIAQA
                     VEDGLSGIRSETPKPEDPIFDLCSTMLVGTNYHAIQEAEQRSKELGYNTLVLGSRFSG
                     EAREIGKVFSGIAQDIILHRTPLCAPACIIAGGETTVTLRGKGKGGRNQEMALSVLNE
                     FDHFSRSVRPGLERTLFLSAGTDGNDGPTDAAGAFADVGALENAHRLGLNASEFLANN
                     DSYTFFEEVGGLFVTGPTGTNVCDIQLLIVK"
     gene            complement(5207..5917)
                     /locus_tag="PK105_06820"
     CDS             complement(5207..5917)
                     /locus_tag="PK105_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU61462.1"
                     /translation="MNKFYANICERFGLNAYVMGDYQKAESWFRKLEQSEPDSIRVLR
                     NLGIISLALGRAEEAERYLLREEKLYGPSFYRNSALGDLAYATGKRKEAEKRYRLALK
                     DPECAKGGKAFHMRDFIERRLAMTEDDKRFCDAIKAMELFKKAEELRESSEHIKAIEY
                     FMKSFALDETNWPALNNAASIHLNMLKNPAEAEPLFRQAFELSRSPQVARNLDLCIQA
                     LNQNQKKEGQKKRWRQRT"
     gene            complement(5938..7269)
                     /locus_tag="PK105_06825"
     CDS             complement(5938..7269)
                     /locus_tag="PK105_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HOU61463.1"
                     /translation="MVSGIVALILLLLAVIAIVVLTGKYRINAFLVLIGVSFVFGLLI
                     GLPALDVVVGIKNGFGGTLTSIGIVIVAGTILGTILEKTGAALSMTRAILNMVGKSHA
                     PLAMNIAGYIVSIPVFCDSGYVILNPLNKALAKESGISMTVMAVALSTGLYATHTMVP
                     PTPGPIAAAGTLGADLGKVILLGLIAAIPASLSGLLWATKFAKRYEIEPEIQGTYSEI
                     VKKFGKLPNTLLSFLPIVLPIVLILLKSVAEFPSKPFGNGDFRVFLSFIGDPVTALIL
                     GVLCSLLLVHKGELKKAISDWMGEGIKESAIILVITAAGGSLGAIIKASPITDFIKTN
                     MASMHLGILLPFIISAALKTAQGSSTVAIVTTAGIMAPLLQTLGLDPALTTIAIGAGS
                     MVVSHANDSYFWVVSQFSGMPVNTAYKAYSSATAVEGVVAFLMVLVLSLFV"
     gene            complement(7464..>7833)
                     /locus_tag="PK105_06830"
     CDS             complement(7464..>7833)
                     /locus_tag="PK105_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61464.1"
                     /translation="ESSELAESHSQVGIINGEGIDSYSIKHGKGVDFDEKIELYTPWA
                     EYPDKFSLAYRDKREQYDGFLVSQSLKDGVGLEYQGFYIADAPMLFTAEGVPREWKGT
                     DGYSDHLPVELVLSVVDGTQ"
BASE COUNT         2114 a         1949 c         1747 g         1923 t
ORIGIN      
        1 tgccaactga gctacagggg cgcaaataga gcttggcgaa tatatcactc atgtggggat
       61 gtgtcaagat tgtgatgcgt tttttccgcg gctcctgcga gacaatgcag tcgttattga
      121 agcagcaact gtatcttttg tcaacaccag tagagcaatg ccgatagctg caaatacgag
      181 tgtcgaggtt acaagaggaa gacccgcata tacgaacctc gagccatatt gcgagaccgc
      241 tttgaacaca gccttatcca caagtatcgc tggcacaata gcgatagctg agaatatgag
      301 catttttgac atatatttta gagaaagcca taactcgttc cttataccct cgatgctatc
      361 cttaataagc gcccacacca gcaatactgt atttacagcg ctcgaaaagg aaagcgcaaa
      421 agctattcct gcaccttgga atctaaaggc cagcaataga acaaggatga tatttacggc
      481 aaaactcgct attcccgcaa atgttggcgt cttggtattg cttcgagcat aaaacgctgg
      541 ggcgattagc ctattcatcg caatgaagac gaggccagtt tgatgccaga aaaaagcgct
      601 cgccgtgagc tgaaccgaga catcggaaaa atcgccggtc ttgaagagca gcgttacgat
      661 cctaaccgta aatgccatgg aaaaaaccgc cacggggacg gtgagcaaga gcaaagaccg
      721 catggtattg ccaaggatct ttgaatactc tgcccaatct ccctgtctta cagtatcggc
      781 caggcgaggc aaaagcaccg ttcccgcaga aaccgcaaag atgcccagaa tcaactcttg
      841 aaggcgcagt gaatactgaa ggctcgatgc tactcctaca ccggcacgcg aagcatatgc
      901 ggtgctcaca agatcgttta cctgataagc ggccattcct agaattgttg gcgcaataag
      961 agcaaaaacc ttgcgcatcc cggaattcct aagagcccgg attggattca tgagccctat
     1021 atgagtgctc agcctcaata cggcaggcag ttgaactaag gcttgggcaa ggccgcccaa
     1081 tacaacaccg acagccatgg atctagcggg attaccaaga cgatctccga gaatccacgg
     1141 caccacaata aaacatacat tgaatagaat tggcgcaaat ccggatggcc caaatatttc
     1201 gtgagcattc agaatgcctt gcaaaagagc ggcaaaagaa acaaatgcaa ggaatggaaa
     1261 catgatacgc gtgaggatag ctgtttccat ggggtctgaa ccaaacagct tgactattat
     1321 aggtgcaagg gcaattccta tcgccactac gatcgtcaca cagatgctca aaatcgtgag
     1381 gcttgcggaa aggaattcgc gagtttcgct atcattctgg gcatgaagat aacccttgta
     1441 tgtggggatg aaagctacag ttacggagcc ttcggcaaat aggcggcgaa gaaagttggg
     1501 aataatgaaa gagaccgtaa aagcatccga atagatcccg gttccaagaa agcgcgcctt
     1561 ggtcatctct cgaatgaggc cgagcacacg cgaaataaag gtaaggagcg aaagtttacc
     1621 agcatttcta accatctttg cagaagcctg cgannnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnttgattg ttcgaattct cttgattgtt taaagtcatt taggctagcc
     1801 tacttcaagc gcgcacaaag gtgaagtcct gtgtaatgga ttttgattgc gacgcgcgca
     1861 gtgttcatag cgagcctgtt ggggctgcgc tccagcgaac gccgccgagg aagtgcgctc
     1921 agcagcacgc cttgtatcta caaagaccaa cgaacaagat gctgtgtcag atatccccgg
     1981 acagatcatt ttcgactaat tcggtagggc tgcgaggaat tcgattagat actgctggat
     2041 gccaagaaag aaacgctctt gaagttctac ggcgggcgga gaaatatagc gatagtgcca
     2101 gctctcccat ttatagccgg ttatattctc catatccttt ggaaaggaaa gcgaaaatcc
     2161 gaattttcct gcattcgatg caagccagcg ctcggcatcg ctgcccgcga attcatcact
     2221 tatggatcca aaatccacga cggtgccaag ctggtgctga gacatcccag ggaccgcgga
     2281 tacacgctca gcttctgctc tccccatttc attgacattc tgctcaaata cgatcttctg
     2341 atagtcgtag gagcgatagg cagaggagac aacgagggca atcccgtcct tttttgccat
     2401 ttctgccatg cggacaagcg cttcttgcgc tggcttgcgc aatttcatgc ctttcttaga
     2461 aacgatatat gggaacggtc catctagatc tacaagatcc atgggcacat actgagcgga
     2521 caaggctttc tgcttatcaa cccgacacag aaggtttgta tcatttttca tgatttcgcg
     2581 tacttcgcta aaaagcactg caaagccagc tgggtcatta tttattgcat cccgcacacg
     2641 attccggtcg gtatcgctga attgcaccac ggaaagggct ttttccactt gatcacggat
     2701 aaccgaaggc aaggaagaaa gacgattatc cttctgaccc gaacacgata ctattacaac
     2761 caatataaat gccaatataa aagtagttgc cagaaaagac agggctcgca tgacaaaatc
     2821 ataccatgga tcgagataat tttaaactct ggtcaggatg ccattagaat aagactatcg
     2881 gacgctggct tgagattcta aggagagtac aagatcgggc tgagtcacct tcgcttcatt
     2941 ggctttgtgg cagcctctat tccattagct tcctagatct tagtttttcc ctcgctgttt
     3001 taatctaaga tagacctgct tgcctggcac catggggtta gtacgctcat caatctcgaa
     3061 tagattcaca gccttaatca gctcgcccaa ctttctaaaa ccataatttc gaggatcgaa
     3121 ctccggaagg cgatttgcga gataggttcc cacttcgcca agataagccc atccgaactc
     3181 gtctgctgct tcatccacag cgcttgcaag caaggctttc atctttgtat cgctctttat
     3241 gtcggcctgt ggcaaagaac ctttgccgcc ttccttttcc gacgagcttt cgcgcccttc
     3301 ctctgtttcg ttgacattgg cttgcagaat ttcggtataa ataaatttgt cgcaggccgc
     3361 tagaaaaggc ttgggcgtct tgcgctgacc aaaaccgagt acgagcttac cgtcctcacg
     3421 cagccttgca gcaagcctcg taaaatccga atcgctggac acaaggcaaa aaccatcgag
     3481 attttcggta tacaagagat ccatcgcatc gattatcatc gcactgtctg tagaattctt
     3541 gccggttgta taagcaaact gctgaacggg ctgaatggca tattctagca atctctcttt
     3601 ccagccccta agagtcgggt ttgtccaatc gccatatata cgctttacac tggccacacc
     3661 atatttggca atctccgcga gaaggccatc gataatagtg ggctgagtat tgtctgcatc
     3721 aatgagcacc gctaatttta gttgcgtatc actcttcata aataatctcc aaaagcaatt
     3781 tatttcacta taagtagttg aatgtcacat acattcgtgc cggtcggtcc ggtcacaaaa
     3841 aggccgccaa cctcttcaaa aaaggtgtag gaatcgttat tggccaagaa ctccgaagca
     3901 ttcagcccaa ggcgatgtgc attttcgaga gctccaacgt cggcaaaggc tcctgcagcg
     3961 tcggtagggc catcgttgcc atcggtgcca gccgataaaa agagcgttct ttcaaggcct
     4021 ggacgaacag agcgcgaaaa atgatcaaac tcatttaaca cagagagggc catttcttga
     4081 ttccttccac ccttgccctt gccgcggaga gtaaccgtcg tttctcctcc ggcaatgata
     4141 catgcgggcg cacaaagtgg agtcctatgc agtataatat cctgagcgat tccagaaaaa
     4201 accttgccga tttctcgagc ttcgccagaa aagcgacttc caagcacaag agtattatat
     4261 cccagctctt tgcttctctg ttcagcctcc tgaatcgcat ggtagttggt tcccacaagc
     4321 attgtcgaac ataaatcgaa gattggatct tctggcttcg gagtctctga ccgaatacct
     4381 gaaagtccat cttctacggc ttgagctatg gactttggca gcctgtccaa gagcgcatat
     4441 ctttcgaata tggctttcac atccctccat gtactcgtat ctggaaccgt aggaccagag
     4501 gcaatggtat ccagatcatc gcccatcaca tccgaaagga ctagtgcaat tatccgtgaa
     4561 gggaaaaaag cctttgccag atggccgcct ttaatggaag atagatgctt gcgtactgca
     4621 ttgatctcat gaatgttagc tccacagccc aataggagcc ttgtcacttc cgctttatcc
     4681 tctagcgaaa ttccgggcgc aggtaccgcc aacagagcgc tccctccacc agagaccagt
     4741 acaaatacaa gggtcctttc gcctgcttct tcccattttc gcacccgcaa agcaaggttt
     4801 agcatttcgc ttccggcttg caccgatctt ttatccggaa cggggtgact tgactcgatc
     4861 gaagtgatcg atcgggacgc aaagagactt tcgatatcct cagagagcct cgaactttgt
     4921 ccactttccg gggccttaga gaccacaagc ccttctgtaa ctcgttggcc tagaatcgag
     4981 gccaggcttg cggtcatagg aatcgaagcc tttccaaaag caactatcac aacatgatca
     5041 tatttggata aatcgtaggc ttgaggctct tctccatccc aaaggcgcag aaccgatccc
     5101 tccagcgaaa gacaatagga aaaaagccgt cccggcataa cccgatccac cgctgctcgg
     5161 aagatctcct ccgcatcggc gcgcaaggag tgtccaggac tttcgcttac gtgcgttgtc
     5221 tccatctttt tttctgtcct tcttttttct gattctggtt gagcgcctgg atacagaggt
     5281 ctagatttct agctacctga gggctgcgag agagctcgaa agcctgccga aataagggtt
     5341 ctgcctccgc aggattcttt agcatgttca aatgaatcga agcggcattg ttgagggcgg
     5401 gccaatttgt ctcatccaga gcaaaggatt tcatgaaata ctcaatggct tttatatgtt
     5461 cgctcgattc tctcaattcc tctgctttct tgaaaagctc catggctttt attgcatcgc
     5521 agaatctctt atcatcttcg gtcatcgcga ggcgccgctc tataaaatct ctcatatgga
     5581 aagccttgcc acctttggca cattccggat ctttgagcgc aagccgatat cgcttttctg
     5641 cctcttttct cttgccggtt gcgtatgcaa gatcgccaag cgctgaattg cgatagaaag
     5701 atgggccata gagcttttct tctctcaaaa gatagcgttc tgcttcttcc gctcgcccta
     5761 gcgcaaggga aattattccg aggtttctca aaacgcggat tgaatccggt tccgattgct
     5821 cgagtttccg gaaccagctc tctgcctttt ggtaatcccc cattacatag gcattaaggc
     5881 caaaacgttc gcagatattt gcatagaatt tgttcatcgg cttcctagat tatcccacta
     5941 gacaaacaag gacaatacga gcaccatcaa gaatgcgact actccttcca cggcagttgc
     6001 agagctgtaa gccttatagg ctgtatttac aggcatgccc gagaattggg agaccaccca
     6061 aaaataggaa tcattagcgt gcgaaacaac catcgagccc gcgccgatcg ctatggtcgt
     6121 aagggctgga tctaggccga gcgtctgcaa aagaggcgcc ataatgcctg cagtggtaac
     6181 aatagctacg gtagaagaac cttgggcggt tttgagggca gcggaaatta taaagggcaa
     6241 cagaattccc agatgcatgc tcgccatgtt tgtctttata aaatctgtta tcggacttgc
     6301 cttgataata gcgcccagtg agccgcctgc tgcggtgatt accaggatga tagcggattc
     6361 ctttattccc tcccccatcc agtccgagat agccttcttt agctctcctt tatgaaccag
     6421 aagcaaggaa cacagcacac cgagaataag cgcggttaca ggatcgccaa taaaagacaa
     6481 aaacacgcgg aaatcgccat taccaaatgg cttagaaggg aattccgcaa cactctttaa
     6541 tagaatcaac acaatcggaa gcactattgg caaaaaggaa agaagtgtat ttggtaattt
     6601 gccgaatttt tttactattt ccgaataagt accttggatt tcgggctcga tttcatatct
     6661 cttggcgaac ttggtagccc agagaagtcc ggaaagagag gctggaatag ctgcaataag
     6721 gcccaagaga atgactttcc ccagatccgc tcccaatgtt cctgcagcag cgataggccc
     6781 cggagtgggc ggtaccattg tgtgggtcgc gtatagccct gtagaaagcg caacagccat
     6841 cactgtcatc gaaatcccgg attcctttgc aagtgctttg ttgagtggat tcaaaataac
     6901 atagccggaa tcgcagaaaa ctgggatcga tacaatataa ccagctatat tcatggccag
     6961 aggagcatgc gatttgccta ccatgttcaa aatagccctg gtcatggaaa gcgccgcacc
     7021 ggttttttcc agtattgtgc ccagaatagt gcctgcaact attacaatgc ctatactcgt
     7081 tagagtgccg ccaaatccat tcttgatccc cacaacaaca tccagggctg gtagcccaat
     7141 cagaagtcca aagacaaaag acacaccaat aagtaccaga aaagcgttta ttctgtactt
     7201 cccagttaag accacaatag ctattaccgc taacaacaag agaattaagg cgacaattcc
     7261 agaaaccata tacacctcca aatatatagt ttactttatg atgcttctag ctccgataat
     7321 gacgtctcat tacatcccat agggcgcgat ccggattccg agattaaacg attctaaggc
     7381 tcgaaagctc cagggataca gcgctaatgc ctttccacat aattgtcatg tatcctgatt
     7441 ttccccacag ataaagaaag tcattattgc gtaccatcaa caaccgaaag aacaagctca
     7501 acaggcaaat gatctgaata tccatctgtt cccttccatt cgcgaggaac accctcggca
     7561 gtaaatagca tgggagcatc tgcaatatag aacccctgat attcaagccc aacgccgtcc
     7621 ttgaggcttt ggctgactaa gaagccatcg tattgttcgc gcttatctct ataggcgagg
     7681 ctgaacttgt ccggatactc cgcccatgga gtgtagagct ctattttttc gtcaaaatcg
     7741 acacccttgc catgttttat ggaataagaa tcgattcctt cgccatttat tatgccaact
     7801 tggctatggc tttcggccag ttctgagctt tcg
//
LOCUS       DAOOWY010000086         7763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_9380_length_7763_cov_10.476129, whole genome shotgun sequence.
ACCESSION   DAOOWY010000086 DAOOWY010000000
VERSION     DAOOWY010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9380_length_7763_cov_10.476129"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..1051)
                     /locus_tag="PK105_06835"
     CDS             complement(344..1051)
                     /locus_tag="PK105_06835"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOU61465.1"
                     /translation="MSYCPECGVEIGNAKRCPLCGTLNPKACEKENAVVYSVNDQKNP
                     EEKTLPQEKPTILWEILSVTLAIAILVLGAIDLFESRRFSWSLYAIVSILFLWVELTA
                     FFMLRKQPVLRILLSVLAPPLLFVIIGYISGNQRWALGLAVPIIMLVESLGGLVYLIF
                     EKSKKKGLNLIAYITIVAAMFCIGLEVLIDLFAIGMVVLRWSPVCAIALLSIAIFFLY
                     LHFRVFKNADLHRLFHF"
     gene            complement(1074..2450)
                     /locus_tag="PK105_06840"
     CDS             complement(1074..2450)
                     /locus_tag="PK105_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61466.1"
                     /translation="MNKNRQSTVSHRFVHTRREDNAGEWYALDNAAIIMPAVTDDVST
                     SLFRFEAELDAPIDPEILKKALALTVNRMPYFNVRLRRGFFWYYFEPCKTMPSIYPDL
                     DFPCQGWNINRHGTRMFRILWKRNRISGEFSHALTDGTGGIGFFKSLLANYLILKGVD
                     PGARLGEGEWKDIAVSSGEMSGLAISEEFEDAYQRYFPNGLPFPDLRPHAFHLSKSCL
                     PKGQYRIISGKLLTSEVIAEAHRRNISLTELMSAVYLDALQHIWYATNPPPREPYVSV
                     EIPINLRQLFPSRTFRNFSLFVLISEDMRLGMRDFDELVLRTHYQMKLEYDRMSISRH
                     LCRNAGSARNLAVRLVPLRIKDFFARVFFSKFGESMLSGFISNLGQLKMPPGFVPHIL
                     SFGFIPAPSMVTMTNASMLSWGNELIINFGSLAQSRELEQLFFRRLRSIGLHVSVSCR
                     SDNGISED"
     gene            complement(2458..3153)
                     /locus_tag="PK105_06845"
     CDS             complement(2458..3153)
                     /locus_tag="PK105_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOU61467.1"
                     /translation="MIATLINAAAIILGSLIGLLIRKGIKDEYRKVVFTAAGLTSLAI
                     GIQMVLETDHILSFALALMVGGLLGTAFDVEGAIERLGDGLKKRFARRSEDTFATGFL
                     NASVLFCAGAMTIVGSFNAGTVGDYSLLLTKSVLDGFVSIIFAGAMGIGVAFAALSVF
                     VYQGALTLLSEYIKPYVSDLMLGELTAIGGALVIMIALGLLEIKSFKTGNFLPAMAVI
                     IILVLAMPFITFL"
     gene            3212..3622
                     /locus_tag="PK105_06850"
     CDS             3212..3622
                     /locus_tag="PK105_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61468.1"
                     /translation="MHKSLLSSVALCALSLVGILVFSCASSPPPVPEDASAQKIIQLA
                     QERYDVYDLNGAKYYYQVLLERYGSDPNYMLNAKYEIAFIEYKKGNKDIAIAGFKEII
                     ERYNEPDASSLSPTWKVLSQMMIEKLEGNKNIQD"
     gene            complement(3672..5654)
                     /locus_tag="PK105_06855"
     CDS             complement(3672..5654)
                     /locus_tag="PK105_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU61469.1"
                     /translation="MPFLQLSGIRLSFGARDLILNASLNLQDGSRAALTGPNGAGKST
                     LMKIAVGLVKPDAGEVIATKDTRIAYLPQTGVQFESGTVYEIAEAAFAFDHDLIKEQE
                     EIGRLLDSSHLSEKETKRLIARHHEITEKLEQAEYWNRGERISEVLNGLDFKIKDFGR
                     SASELSGGWQMRLALAKILLEEPDIMLLDEPTNYLDLEARTWLEDFLKAYPGAVLVVS
                     HDRYFLDVTVREVYELFNGCLTRYVGTYSQYEMRRSQELEALFEAWERQQEERQRIED
                     FIRRFRYKDSKAAQVQSRIKMLEKMTPIEIPEGMKRIHFSFPPAPRSPRVVLRIHQLS
                     RSYGELRVIEPFSLEVERGQKLALVGPNGAGKSTLMRIIAKEDNPTSGEAILGANVLV
                     GYFSQESAEIMESTATVKREAESVCPPEMLPKIRNLLGAFLFRDDDIDKPISVLSGGE
                     RSRLALLKLLLKPANLLVLDEPTNHLDLTSKDILLEALSRFEGTVILVSHDRQFLDEL
                     PDRILELSCGNRPLLYYGNYAYYLTKKAEKENAKLEAMQEDNQKPKQRRQSETDSASS
                     VMHQELISDKNWEAEKARKARLRKLKRREEDISTRIEEIQTEKEKLQEELAQPRNYIN
                     GEKTRRILAELEDLDRETEALNEEWLEIAQSLSEEA"
     gene            complement(5667..7172)
                     /locus_tag="PK105_06860"
     CDS             complement(5667..7172)
                     /locus_tag="PK105_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU61470.1"
                     /translation="MVYYSIFVPTKNTSKNLLLIVHSRCMNFEERRDQGISKGVFSIV
                     GLVLILFCILLASCARLEGWGLVIWSVKGTTAKAGSIVPVYLKSNISKTYMIGLPDDS
                     KTKIEVPFATIEFFASKKAAEKRAREFAPYALYYLSAGRDGLPVRDKPSVTGRRVYRL
                     MIGESVKVLNRVEGEAVFTGGKALPGEWYFVQAADGTRGYVFSNTMILYEEKEGVSVP
                     VLTTAPTPSVSLLDMIYAKPWRPAYFQLMLDEDTVDPDLFLLQYGLFADAKNSQVRIE
                     LPGYSKAFSFGSVSQIGDWLVFEGSNLRIKFENESTIVADWSGLEPMLPEEGWTPGGQ
                     AARFVRFDASIPVIISGENSRRDSELKVFFQRVSQLQTNGSSSSATFLSDHAGILTID
                     LKGNFEWSHIDQLPAGFVPEVSLGSGSGSDGNVLKGRIRFGLHLETQLSSYWMGGFSL
                     FMGEEPQRYDFLYRFENEQLIMAKAIQVSLRGTTESIDSRFSPVSFYLISK"
     gene            7173..>7763
                     /locus_tag="PK105_06865"
     CDS             7173..>7763
                     /locus_tag="PK105_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOU61471.1"
                     /translation="MSETPSQKSLELKENYVNSPGIHAAIEMGSTGIRLIIAEIDSKG
                     DLKILDRASKQSRIGRDVFTMGSISRDGMRETIAILDSFSELLAGYDLHPSDIQVMGT
                     SALREASNRDTFMDRINLQTGFRVRIIEDIEENHLMYLAVKKALEDERSYLSRSNAMI
                     LEVGGGTTELMILRKGQMVSSHSLSIGTLRIDEQIRE"
BASE COUNT         2150 a         1866 c         1680 g         2067 t
ORIGIN      
        1 gacctccggg ttatgagccc gacgagctgc caactgctct accctgcggc gcaaaaatta
       61 atctacaata aatcgcaaaa tatgtcaagg gataggaaaa ttttctgata caatggtgag
      121 ttttcactat caggatttgg agatagcggc ccagtttggt ttaggtcagg ttagcctaag
      181 ccaaattagg tcgatcgctg tttgcgcgta aggcgcgccc gcagggttag tttaagtcag
      241 gttagtctaa gccaaattag gctcggttcg gctgcatcat actgacctaa cttagctttg
      301 tttcgtcaaa tccgccttgc ttgccaaatt gaagcatgaa gcatcaaaaa tgaaaaaggc
      361 gatgcaaatc cgcattcttg aaaacgcgaa agtgaaggta taagaagaag attgcgatgg
      421 ataagagagc aatggcgcag acaggagacc atcgcagaac caccatacca atcgcaaata
      481 aatcgattag cacttccagg ccaatgcaaa acatcgccgc cactattgtt atataagcaa
      541 ttagattgag acctttcttc ttgctctttt cgaatataag gtaaaccaag ccacctagag
      601 attctacaag cattatgatt ggaaccgcca gaccgagcgc ccatcgctgg ttaccagaaa
      661 tgtacccaat tattacaaat aataagggtg gtgcaagaac gctcaataag atacgaagaa
      721 cgggctgctt tctcaacatg aaaaatgctg tcaattccac ccatagaaac aaaatggata
      781 caatagcgta gagagaccac gagaatcgcc tggattcaaa aagatcaata gcgccaagca
      841 ccagtattgc tatagccaat gtcacagaga gaatttccca aagaatcgtc ggtttttctt
      901 ggggcaaagt tttttcttcg gggttttttt gatcatttac cgagtagaca actgcatttt
      961 ccttttcgca agcctttggg tttaaggttc cgcagagtgg gcatcgtttg gcgttgccga
     1021 tttctacccc gcattcggga caatacgaca tgattcaccc cccatttttg ccttcaatct
     1081 tcactgattc cattgtcaga tcgacatgaa acactgacat gcagtccgat acttctcaat
     1141 cgtcgaaaaa acaattgctc gagttcgcgc gattgcgcca agcttccaaa attgataata
     1201 agttcattgc cccaacttag cattgaggca tttgtcatgg taaccatgct tggagccggt
     1261 ataaatccaa agctcaatat atgaggaaca aaacctggag gcattttcaa ttggcccaag
     1321 ttagaaatga aaccagaaag catactctcg ccaaatttcg agaaaaagac cctagcgaaa
     1381 aaatccttta ttctaagagg aaccaatcgg acagccagat tccttgcact tccggcatta
     1441 cggcacaagt gtctagatat gctcattcga tcatattcga gcttcatctg atagtgagtc
     1501 cgcaaaacaa gctcatcgaa atctctcata ccgagccgca tatcttcact gatcagcaca
     1561 aagagagaaa aattgcgaaa ggttcgagaa ggaaaaagct ggcgcagatt gataggtatc
     1621 tctactgaga catagggctc acgcggaggg ggattagttg cgtaccatat atgctgcaag
     1681 gcatcgagat agacggctga catgagttcg gtcaagctga tatttcttcg atgagcctca
     1741 gcgatgactt cgctggtaag caacttccct gaaattatgc ggtactggcc tttgggcaag
     1801 caggatttgc tcaaatgaaa agcatgcggt cttagatccg ggaatggcaa tccattggga
     1861 aaataccgct gataagcatc ttcgaattct tcgcttatcg ccaagccact catttcacct
     1921 gaggacacag ctatgtcttt ccactcgcct tctcctagtc gtgcacccgg gtcgacaccc
     1981 ttcaatatga ggtagtttgc aaggagagac ttaaaaaaac ctatgcctcc cgtaccatca
     2041 gtcaaagcat gagaaaactc acccgaaatt ctgttccgct tccaaagaat gcgaaacatg
     2101 cgcgtcccat ggcgatttat attccatccc tgacaaggaa agtccaaatc cggataaatc
     2161 gacggcattg ttttgcaagg ctcgaaataa taccagaaaa atcctcttcg gagccttaca
     2221 ttgaaataag gcatgcgatt cactgtaaga gcaagcgcct ttttcaatat ttctggatct
     2281 atgggcgcat cgagctcggc ttcgaaccta aaaagactcg tgctgacgtc atctgtgact
     2341 gctggcatta tgattgcagc gttgtcgagc gcataccatt cgccagcatt atcttcgcgc
     2401 cgagtgtgga cgaatctatg gcttactgtg ctctggcgat ttttgttcat gtttcgatta
     2461 tagaaatgta ataaatggca ttgcaaggac gagaattata atgacggcca ttgccggaag
     2521 aaaattaccg gttttgaagc ttttgatctc caagagcccc agggctatca tgattacaag
     2581 cgctcctccg atagccgtaa gctcgcccaa cattaggtca ctgacatagg gctttatgta
     2641 ctcggatagt aaagtaagcg ctccctgata cacaaaaaca gaaagagccg caaatgctac
     2701 accaattccc atagccccag cgaatataat tgagacaaaa ccatcaagca cgctctttgt
     2761 caggagaagc gaataatccc ctacggttcc cgcattgaat gaacccacta ttgtcatggc
     2821 gcccgcacag aacaaaaccg aagcattgag aaatcctgtt gcgaaagtgt cttcggacct
     2881 gcgcgcaaag cgttttttaa gaccatctcc tagcctctct atcgcacctt cgacatcgaa
     2941 ggcagtaccc agaagtccac ctaccatcaa tgcaagggca aaagaaagaa tatggtctgt
     3001 ctctagcacc atctgaatac caatagcaag cgatgtaagc cctgccgcgg taaacacaac
     3061 ctttcgatat tcgtctttta tgccttttcg tataagaagc ccaatgagtg aacccagaat
     3121 gatggccgct gcattgatta atgttgcgat catggcttgt cttgacctcg tttggcgagt
     3181 atagcataat tgccacttgt gaggaaatga catgcataaa tcgcttttaa gctctgtagc
     3241 cttatgtgcg ctctcactcg tcggaattct ggtattttct tgtgcctctt cgcctccacc
     3301 ggtgcctgaa gatgcaagtg ctcaaaagat catccagcta gcccaggaac gctacgatgt
     3361 ctatgatctc aatggcgcta agtattatta tcaggtgctg ttggaaagat atggcagcga
     3421 tccaaactat atgctcaatg ccaagtatga gatagctttc attgaataca agaaaggcaa
     3481 taaggatata gcgattgcgg gttttaaaga aataatcgaa cgctataatg agcccgatgc
     3541 atcgagtctt tccccaacat ggaaagtgct ctcccagatg atgatcgaaa agctagaagg
     3601 caataagaat attcaagact aatagctata gaccgcgctt tttccaattc aaaggagctt
     3661 atttcaagcc tttaagcttc ctcggataaa gactgcgcaa tctcgagcca ttcttcattt
     3721 agagcttcgg tttctctatc gaggtcttct aattctgcca ggatgcgccg cgtcttttct
     3781 ccatttatat agtttcgagg ctgagccaat tcttcctgga gtttctcttt ttctgtttga
     3841 atctcctcaa tgcgagtaga aatatcttct tctcgtcgtt ttagcttgcg taatcgcgct
     3901 ttgcgggctt tttccgcttc ccaattctta tccgaaatta gctcttgatg catgacggaa
     3961 gaagcagaat ctgtctcact ttgccggcgc tgtttgggct tctgattatc ttcttgcatc
     4021 gcttctaatt ttgcattctc tttttcggcc ttcttggtca aatagtaggc ataattccca
     4081 taatagagaa gcggtctatt gccgcaagag agttccagaa tccgatcagg aagttcatcg
     4141 aggaattgac gatcatgaga gacaagaatt accgtgcctt caaatctact cagagcttcg
     4201 agcaaaatgt ctttactagt caaatccaga tgattagtcg gctcatctag caccagaagg
     4261 tttgcgggct ttagaagcaa cttgagaaga gccaaccgcg agcgttctcc tccagataac
     4321 actgagattg gcttatcaat atcgtcatcg cggaaaagaa aagctcctag cagattgcga
     4381 atctttggaa gcatttcggg tggacagacg gattcggctt ctctttttac cgttgcggtc
     4441 gattccatga tctccgctga ttcctgtgaa aaataaccca cgagcacatt ggctcctaga
     4501 attgcttctc cgcttgttgg attatcttcc tttgcaatta tgcgcatcag cgtagatttc
     4561 cctgcgccat ttggacctac aagggccaat ttttgtccac gctcaacttc gagagaaaat
     4621 ggttctatta ccctgagttc tccataagac ctggagagct gatgaattcg caagaccaca
     4681 cgaggggatc ttggtgctgg cggaaaagag aagtggatcc gcttcatacc ctccgggatc
     4741 tctataggtg tcatcttttc caacattttt atacggctct gtacctgagc agccttggaa
     4801 tccttgtatc taaaacgccg tatgaaatct tcgattcttt gcctttcttc ttgttggcgc
     4861 tcccaggctt cgaagagtgc ttccaattct tgagatctgc gcatttcata ctggctatag
     4921 gtccccacat agcgtgtaag gcaaccattg aatagctcat atacttcccg tacagtaaca
     4981 tcgagaaagt atctatcatg tgagactact aataccgcac cagggtaggc tttaagaaaa
     5041 tcttccagcc aggttctagc ctctaggtct aaataattgg ttggctcatc cagcagcatg
     5101 atatctggct cttctagcaa tatctttgcc agcgcaagtc gcatctgcca tccacctgat
     5161 agctcagacg cagaacgtcc gaaatccttg atcttgaaat ctagcccatt caaaacttca
     5221 gaaatcctct cccctcgatt ccagtattct gcctgctcga gtttctcagt gatttcatga
     5281 tgccttgcaa taaggcgttt tgtttctttt tcgcttaaat gagaggaatc tagtagtcgc
     5341 cctatttctt cctgttcctt tataagatca tgatcaaacg caaatgcggc ttcggcgatc
     5401 tcatagacag ttcctgattc gaattgaact cctgtctgtg gaaggtaggc tatgcgggta
     5461 tcctttgtgg cgatgacttc ccctgcatca ggcttgacaa gtcccacagc tattttcata
     5521 agtgtcgact tgccagcacc gttgggtccg gtcaatgccg cacgagaccc gtcctgcaga
     5581 ttcaggctcg catttaatat gagatctctg gcaccgaatg agagcctaat tccgctcagc
     5641 tgcagaaagg gcatgctatt acaaccttat ttacttataa gataaaagct tacaggagag
     5701 aaccttgaat cgatactctc cgtagttcct cttaggctca cctgaatagc cttggccatg
     5761 ataagctgtt cattttcgaa gcgatataag aagtcatatc tctggggttc ttcacccata
     5821 aacagtgaaa aaccacccat ccaataggaa gaaagctgag tctctaagtg gaggccaaat
     5881 cgaattctgc cttttaaaac attgccgtct gaccccgagc ccgatcccag cgagacttcg
     5941 gggacaaaac cagcaggaag ctggtcgata tgactccatt cgaagttccc tttgaggtct
     6001 atagtcagaa tccccgcatg gtcagatagg aaggtcgccg aagaagagct gccgtttgtc
     6061 tgaagctgtg agactctctg gaaaaagacc ttcaattccg aatcgcggcg tgagttctct
     6121 cctgagataa taaccggaat cgatgcatcg aagcgcacga aacgagccgc ctgtccacca
     6181 ggagtccagc cttcttcggg gagcataggc tctagaccac tccaatccgc tacaattgtc
     6241 gattcgtttt cgaacttgat tctgagattt gatccttcga atacaagcca atctcctatc
     6301 tggctgactg aaccgaaact aaatgcctta gaatagccag gaagttctat tctaacctga
     6361 gaattctttg cgtccgcaaa aaggccatat tgtagcagga aaaggtcggg atccacggtg
     6421 tcttcatcta gcatgagctg aaaataagcc ggacgccaag gttttgcgta tatcatatcg
     6481 agcaatgaaa cacttggagt cggagccgtt gttagtaccg gcacacttac gccctctttt
     6541 tcctcatata aaatcatggt attgctgaat acatagcctc gggtgccatc ggcagcttgc
     6601 acaaaatacc actctccggg gagcgctttt ccaccggtga atacggcttc gccttccact
     6661 ctgttgagaa ctttcaccga ttcgccaatc atcagccgat agactcttct gccggtcacg
     6721 cttggcttgt cgcgcacagg aagaccatct cgccccgcgc tcaggtaata tagagcataa
     6781 ggtgcaaatt cccttgccct cttttccgcg gcttttttcg aagcaaaaaa ctcaatcgtt
     6841 gcaaatggca cttctatttt tgtcttgcta tcgtccggca agccgatcat ataggttttg
     6901 cttatgtttg atttcaggta aactgggacg atagagcctg ctttggccgt tgttcccttt
     6961 acggaccaga tgaccaaacc ccatccttcc aatctcgcac aactggccag caaaatgcag
     7021 aataaaatga gaacaaggcc aacaatcgaa aaaacccctt tactgatacc ctgatctctt
     7081 ctttcttcga aattcataca gcgagagtgt acaatgagca aaagattctt gctagtgttt
     7141 ttggtcggca caaagatgct ataataaacc atatgtccga gacgccatct caaaaatcgc
     7201 tagaactcaa ggaaaactac gtaaactcgc ctggaatcca tgccgctatt gaaatgggtt
     7261 ccaccggtat acgcctcata atcgccgaaa tcgattcgaa aggagacctg aagatactcg
     7321 atagagcatc caaacaatct cgtattggtc gcgatgtgtt tacaatgggc agcatttctc
     7381 gggatggcat gagagaaacc attgctatac ttgactcatt cagtgagctt ctagcgggtt
     7441 atgatctgca tccttcggat attcaggtaa tgggaacgag cgcgctccga gaagcctcga
     7501 acagagacac ttttatggat cgcataaatc tgcagacagg gttccgtgtt cggatcattg
     7561 aagacatcga agagaatcat cttatgtacc tggcagtaaa aaaggcgctc gaggacgaaa
     7621 gatcctatct tagtcgctcg aatgcgatga ttctggaggt agggggtggc actacagagc
     7681 ttatgattct aagaaagggc caaatggttt ccagccatag ccttagcata ggcactctta
     7741 gaatagatga acagatccgc gaa
//
LOCUS       DAOOWY010000087         7511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_9814_length_7511_cov_8.565048, whole genome shotgun sequence.
ACCESSION   DAOOWY010000087 DAOOWY010000000
VERSION     DAOOWY010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9814_length_7511_cov_8.565048"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1310)
                     /locus_tag="PK105_06870"
     CDS             complement(177..1310)
                     /locus_tag="PK105_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61472.1"
                     /translation="MSQLELKEVTKRFGEVIAVNRLSLQVEKGECFTFLGPSGCGKTT
                     TLRMIAGFEDLDEGELLVEDSLYSSSYRKFYVPPEKREFGMVFQAFAVWPHLTVKENV
                     AFPLRIKHRPSSEIESRVKMALTSTNLMKFKDSYPGRLSGGEQQRIALARAIAINPKV
                     MLLDEPLSNLDAKLREEMRFEIKALQNRFGFTVIYVTHDQAEAMALSDRMLVMNYGVV
                     QQVGKPLDIYNKPANRFVFSFIGLSNFIPVMIKEGDAFIEGTKQIASHNVPRELGPRA
                     VMACRPSDITFMPISEIDEEKGSLGVQALVIRRTYLGDIVDLKVKVGSVDLRIQKNAR
                     SPCPREGESCRIRFNKVLWYPEEYPKESQNMTENQPKTSSEAS"
     gene            complement(1297..3027)
                     /locus_tag="PK105_06875"
     CDS             complement(1297..3027)
                     /locus_tag="PK105_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU61473.1"
                     /translation="MKDLSKSLKKIDVSTVILIFSILILVVIVAVPVLLIFLTAFFEN
                     GKFNIAGVVKVLSDPNTYNALLNSFIIAAGTTLLSTVVGTFFAWLVARTDLPFKNTMK
                     ALFLVPFMLPSFIGALAWKVLLSPRSGYLNKLMISIFGLHKAPIDIMSMGGIIVIESM
                     YLLPFVFLQVEGALERMDPTLEESARISGASLFTITRKITLPLVMPSILAGALLVALY
                     SLSHFGVPSILGTEKGIFTIPTKIYERIYASGGSFSAIRVGTILATILVISAALILKL
                     QDVLLKKGQYQVIAGKSMRPVVLKLRGLKYPLLIISFIYIFITIILPTVTIFLVGGLK
                     TYGLPFQLKYMTFDNYLKIFQWKMTKDAIWNSLYLSLSAALVTMLAGTMISYAIVKMN
                     FKGKFILQFLGVLPYSLPGTVIALGVILTWSGRFGINLYNTAAIIFVAYIARYMAFAI
                     KSSSASLSQVSDSLEEAARACGATHWQTLKDIVLPLIRPGMISAFFLIFLPALRELTT
                     SVLLYGPTTRTIGVAIYTLNEDGETVKAAALAGIALIIIFVGETLIRKFLAKLEQRAV
                     RVKEIENVPA"
     gene            complement(3024..4115)
                     /locus_tag="PK105_06880"
     CDS             complement(3024..4115)
                     /locus_tag="PK105_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61474.1"
                     /translation="MADLKLQQIKKAFGDHIILDRFDLHIRSGECFTIIGPSGCGKTI
                     ILRLIAGFETPDAGEVLIGETKVADTHHALPPEERKIGVVFQDYAVWPHKTVYGNVAY
                     PLEIAGVTKSDIAKRVHDALALVNLDGYEMRFPYQLSGGQQQRVALARALVMRPEILL
                     LDEPLTNLDANLREEMRFEIKEIQKATGATILYVTHDQEVAMALSDRLAVMDEAGRIH
                     QIDTPEIIYTHPIDIFVFKFLGISNIISIKVSEHFASAKIEDKTVELPFVAPSSMEGK
                     EGILGFRPMDVQIERKGNSADFPAIATRVTILGPIVDYLFDFQGIPIRAQALTDDALA
                     ADLILEPGEKVFLSVLEPKWFNSEGVAQQ"
     gene            complement(4220..5206)
                     /locus_tag="PK105_06885"
     CDS             complement(4220..5206)
                     /locus_tag="PK105_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61475.1"
                     /translation="MRKWVFIFLILSILTTPVFAQKKEKLMVYTSMKETLIGDLKKGF
                     EKKYPNVSLDYYSAGAGKIMAKIAAERQSGKLAVDVIWTSEVPDFYAMKKEGILQRYE
                     SPEAKNVVSPLFDPDYEFTPARLGTLGIAYNTNKIKTPPKEWTDLLTPEYKNGFGIAN
                     PALSGTAMVSVAMLVDTFGWDFIQKLRANGAKMGEGSGQVVDDTAAGDLVACIGVDYI
                     TIDKIKLGAPLGFAYPKQMLVIPSPVAIFKGTPNLSAAQKFVDFLLSKDGQAIIASNC
                     TLPIRRDVPIAEGVGLVSPEEAVKRSIKIDYIKLIDEKQAIIDKFISIMQSK"
     gene            complement(5348..5740)
                     /locus_tag="PK105_06890"
     CDS             complement(5348..5740)
                     /locus_tag="PK105_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61476.1"
                     /translation="MIRMLIVVPYRELYDLVQDYARDSEMNEIRLEEAPLRSGAPANS
                     YSGKRQATFAPDWSYCLLDGAFEALNRGDTSSHDAEVVSACERYAEQGTRIYFAQISM
                     ARILPALSTKARAAVYTSLDFIEETLGL"
     gene            complement(5790..7193)
                     /locus_tag="PK105_06895"
     CDS             complement(5790..7193)
                     /locus_tag="PK105_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOU61477.1"
                     /translation="MNFGGESYNLGFISRQVLHPKEGQSGRFSRLLVFFFIVLILAIA
                     FIILMILYNPIASDNIEYAERLIEARAQRASELANAIEAITYQFYSNRILNDLLLDYT
                     ASKETYDVSRWNSIFSYFMEGMAEASPELEDAMFFDSSNINKRPLTMTDSLTRLVWNS
                     AKTQAAEIAEKAGGKPIWTFLQISSPSSTDSSETSNYLICARTIKDVSSGEHLGLLIL
                     LVNPERIATTVAGGYWNSDSSIPKTDLSVLVSEEGKILGCIAPRLIGCSAEEIVPGYI
                     SPWPQADRESGRYIINQSILSRPSRNYLVIWRSVPEKSWQLFTVLPYSKHSFKTVMIM
                     LAITLVVAGFFLMLFFLRVKSRSYMSGQISSEAAEVVGDFAYTKQVLPPLSHNEAKIQ
                     KSLSAREQAILAGLAKGLSNKEIASDLNLQEQTVKNYLSNLYRKLGIHDRIEAVFFAI
                     RAGLNEKEPSDISHEDQ"
BASE COUNT         2125 a         1655 c         1590 g         2141 t
ORIGIN      
        1 tagagagagc tcttttggag agctctctcc ttgagaggct tgcgatgcag gcattctttt
       61 gaagtaataa gctttattgg agtgccctct ctttgaaaac aggcgattca cacatattct
      121 ttcaaggaaa atcaaaatca aagattgctg cttatcttag ctgcgaatct tgctggtcag
      181 cttgcctcgg aactcgtttt gggttgattt tccgtcatat tctggctttc ttttggatat
      241 tcctctggat accagaggac tttgttgaag cgaattcgac aagattctcc ttctcgagga
      301 catggagatc gagcgttctt ttgaattctc agatctacgc tgccaacctt taccttaaga
      361 tctacgatgt ctccaagata agttctcctt attactaaag cctggacacc cagagatccc
      421 ttctcctcgt ctatttctga gattggcatg aaagttatat cagaagggcg acatgccatt
      481 accgctctag gtcctaattc tcttggcaca ttgtgcgatg caatctgctt ggtgccctcg
      541 atgaaagcat ctccttcttt tatcataact ggaatgaaat tcgaaaggcc gatgaatgaa
      601 aaaacaaaac gattagctgg cttgttatag atatcgagcg gcttgcctac ctgctgcacg
      661 actccataat tcatgacgag cattctatct gaaagagcca tagcctctgc ctgatcatga
      721 gtcacataga ttacagtaaa accaaagcgg ttctgcaaag ccttgatttc gaaacgcatc
      781 tcttcacgca atttggcatc taggttggaa agaggctcgt caagaagcat gacttttgga
      841 ttgatcgcaa ttgcccgtgc aagcgcaatt cgttgctgtt cgcctccaga aagacggcct
      901 ggatagctat ctttgaattt catgaggttc gtgctggtaa gcgccatctt gacgcggctt
      961 tctatttcac tcgatggacg atgctttatt cgcaagggaa atgccacatt ttcctttaca
     1021 gtcagatgcg gccatacagc aaaagcctgg aataccatgc caaactctct cttttctggc
     1081 ggtacataaa acttgcggta gctagacgaa tacaatgaat cttctacaag caactctccc
     1141 tcatccaggt cctcaaatcc cgctatcatt cgaagcgtag tcgtcttacc gcagcccgaa
     1201 gggccaagaa aagtaaagca ctcgcctttt tcgacctgta gactaaggcg attcactgct
     1261 ataacttcgc caaatcgctt ggtcacctcc tttaattcaa gctgggacat tttcgatctc
     1321 cttaacgcga accgctctct gctctaactt agccaggaat ttgcgaatca acgtctcacc
     1381 tacaaatatt atgatcagtg ctatacctgc gagagctgct gcctttaccg tctctccgtc
     1441 ttcgttcaag gtatatatgg caacgccaat agtcctagta gtcgggccat agagtagcac
     1501 cgaagtagtc aattctctca gagctggtaa gaaaatcagg aaaaaagcac ttatcattcc
     1561 tggtcgaatt agaggtagaa ctatgtcttt gagagtctgc cagtgtgttg ctccacaagc
     1621 tcttgcagcc tcctcaagcg agtcggatac ttgcgataat gaggctgagc tcgatttgat
     1681 tgcaaatgcc atatatcgag ctatatatgc cacaaagata atggccgcag tattatataa
     1741 atttatgcca aagcgcccac tccaagtaag aattactccc agcgcaatca cagtgccagg
     1801 tagcgaatat ggcagaaccc caaggaattg aaggatgaat ttgcctttga aattcatttt
     1861 gacgatagca taagaaatca ttgtacccgc caacattgtt acaagcgcag cagaaaggga
     1921 aagatatagc gagttccaga tggcatcttt tgtcatcttc cattggaata tcttcagata
     1981 gttatcgaag gtcatatatt tgagctgaaa gggaaggcca taggtcttaa ggccacccac
     2041 caagaaaatc gttactgtgg gaaggataat agttatgaaa atatagatga agcttataat
     2101 tagaagggga tacttgagcc cacgaagctt gagcacgact ggacgcatgc ttttgccggc
     2161 aattacctga tattggcctt tctttagcaa aacatcctgt aacttgagta tcagcgcagc
     2221 agagattaca aggattgtag caagtatggt tcctactcgt attgcactaa aagagcctcc
     2281 agaagcgtaa atcctttcgt atatttttgt gggaatggta aaaataccct tctctgtacc
     2341 aagtatagat ggaactccaa aatgcgaaag tgaatagaga gcaacaagca aagcgcccgc
     2401 cagaatgctt ggcataacta agggcagcgt aatttttctt gttattgtga aaagacttgc
     2461 ccctgaaatt cgtgcagatt cttcgagcgt tggatccatc ctctctaatg ctccttcaac
     2521 ttgaagaaat acaaatggaa ggaggtacat gctttcgatt acaatgatgc cgcccatact
     2581 cataatatcg atgggcgctt tatgcaagcc aaagatagag atcattagct tgttcaagta
     2641 tccagatcgc ggcgatagca agactttcca tgcaagtgca ccaataaacg aaggaagcat
     2701 aaatggcaca agaaaaagcg ctttcattgt attcttgaag ggcaaatcag tgcgcgcaac
     2761 caaccatgca aaaaaggtcc cgactaccgt agagagaagt gtcgtgccag ctgcgattat
     2821 gaatgagttc aagagtgcat tgtaggtgtt tggatcagac agaaccttga cgactccagc
     2881 aatgttgaac tttccatttt caaaaaaggc tgtaagaaat atgagcaata ccggcaccgc
     2941 tacaattacg acgaggatca aaatggagaa aataagtatt acagtagata catctatttt
     3001 ttttagcgat tttgataaat ccttcattgt tgtgccactc cttctgagtt gaaccatttg
     3061 ggttcgagaa ctgaaagaaa caccttctct cctggctcga gaatgagatc agctgcaagc
     3121 gcatcatcgg taagagcctg tgcacggatc ggaataccct ggaaatcaaa gagatagtcg
     3181 acaattggac cgagaatggt tacgcgggtc gctatggcag gaaaatcagc cgaattccct
     3241 ttcctttcaa tttgcacatc cataggtcta aatccaagaa tcccttcctt tccttccatt
     3301 gatgatggcg caacaaaagg aagctccact gtcttatctt ctatttttgc agatgcaaaa
     3361 tgctctgaga ctttgatgga gatgatgttc gaaataccta aaaacttgaa aacgaatata
     3421 tctatgggat gggtatagat tatctcaggt gtatcgatct ggtgtatcct gccagcttca
     3481 tccattacgg cgagtctatc ggatagagcc atcgcgactt cctgatcatg agttacatat
     3541 aggatcgttg ctccagtcgc cttctggatt tctttgattt caaagcgcat ctcctcacgc
     3601 agattcgcat cgagattggt aagcggctca tcgagaagaa gtatctcagg acgcataacc
     3661 aaggcgcgtg caagtgcgac gcgctgctgt tgccctccag aaagttggta aggaaaacgc
     3721 atctcatagc catctaggtt tacaagcgct aatgcatcat gaacacgttt tgctatgtcg
     3781 ctctttgtaa ctccagctat ttccagggga taggcaacgt taccatacac tgttttatgg
     3841 ggccaaactg cgtaatcctg aaagacaacg ccaatcttac gttcttctgg tggtagagca
     3901 tgatgagtat ctgccacttt agtttcgcca ataagaactt cgcctgcatc gggcgtttca
     3961 aaacctgcaa tcagtcgtag tatgatcgtc tttccgcaac cagaaggccc aattattgta
     4021 aagcactcgc cgcttctgat atgaaggtcg aatcgatcca agattatatg gtcgccaaat
     4081 gcctttttga tctgctgcaa tttcagatca gccactctat tctcctttca ataagggaat
     4141 ctaaagaagg ctgtggagcg gaaaaaccca ctccacagcc gaaatatagc ccgctaggaa
     4201 tacggccttt cttcgatagc tatttgctct gcataataga aatgaatttg tctatgattg
     4261 cctgtttttc atctatgagc ttgatataat cgatcttgat agagcgcttg acggcttctt
     4321 caggtgatac aagcccaact ccttcggcta tgggaacatc gcgccttata ggcaatgtgc
     4381 aattgctggc tataattgcc tgaccatctt tggaaagcag aaaatctacg aatttctgtg
     4441 ctgccgagag gttcggtgtt cctttgaaga ttgcaactgg actgggaatg acgagcatct
     4501 gttttggata agcaaaacca agcggagcgc caagttttat tttatcgata gttatgtaat
     4561 ctacgccaat acaagcaaca agatctcctg ctgcggtgtc atcaaccacc tgacccgatc
     4621 cttcgcccat ctttgcgccg ttcgcgcgca atttttgtat gaaatcccat ccaaatgtat
     4681 ctactagcat agcgacagag accattgctg tgcctgaaag agcaggattc gcaattccaa
     4741 acccgttctt gtattctggc gtaagaagat cggtccactc ttttggtggc gtctttatct
     4801 tgttcgtgtt gtaggcaatt ccaagtgttc ccaatctggc gggtgtgaat tcataatcag
     4861 gatcgaaaag aggacttaca acattcttcg cttcaggcga ttcatagcgc tgaaggattc
     4921 cttctttctt catggcatag aagtcaggaa cttcgcttgt ccatattaca tccacggcaa
     4981 gcttgcccga ctggcgctct gcggcgattt tggccatgat tttgccggct cctgctgaat
     5041 aataatcaag agaaacatta ggatatttct tctcgaatcc tttcttgaga tctccaatga
     5101 gcgtctcttt catcgatgta tagaccatca gtttttcttt tttctgagca aagacaggtg
     5161 tcgtcagaat agacaaaatt aagaaaatga atacccattt tctcataata gacctcctat
     5221 ggatcgaatg agattgtgcg cctaaaggcg cttatagaac tacagttttg ggttgtttgg
     5281 gcgaattagc aatgagtact ttggtacttc tttcgaaatt ttgtgattaa tgttacgaat
     5341 tgatctctca taatccgaga gtctcttcaa taaaatcgag actggtgtac acagcagcgc
     5401 gtgccttagt agacaaagca ggtaggatac gcgccatcga tatctgcgca aaataaattc
     5461 tagtaccttg ttcggcgtat ctttcgcaag ctgagacgac ttcagcatcg tgacttgatg
     5521 tatcacctcg attgagagcc tcaaaagctc catcgagaag acaatagctc cagtcgggag
     5581 caaatgtcgc ctgcctctta ccagaataag agttggctgg agcgccagat cttaaaggtg
     5641 cttcttcgag cctgatttca ttcatctccg aatctcttgc ataatcctgt acaagatcgt
     5701 agagctctct atagggaact acaattagca ttctaatcat ttgctattac ctatggtgat
     5761 tgtaaaccca gtacattgct tactggaact tattgatctt catgtgaaat atcgcttggc
     5821 tctttttcat taagtccggc tcttattgca aaaaacacag cctcaattct gtcatgtatg
     5881 ccaagttttc gatagagatt tgaaagataa ttcttgacag tctgctcctg tagattaaga
     5941 tcgctagcaa tttccttgtt gctaagcccc tttgcaagcc ctgcaagaat ggcttgctct
     6001 cttgcgctta aagatttttg gatcttggct tcattatgag agaggggagg aagaacttgt
     6061 tttgtgtatg caaaatctcc tactacctca gctgcttcgc tagaaatttg cccactcata
     6121 tagcttcggg attttacgcg tagaaagaac aacatcagga aaaagcctgc aacaacaaga
     6181 gtaatagcaa gcataatcat gactgttttg aaagagtgtt tagaataggg gagaacagta
     6241 aagagttgcc aggacttttc aggcaccgat ctccaaatga caagataatt acgactagga
     6301 cgggatagaa tagattgatt gataatatat ctgccacttt ctctatcggc ttgaggccag
     6361 ggagatatat atccgggcac gatttcttct gccgagcaac cgatcaatct aggggcaatg
     6421 cacccaagaa tcttcccttc ctcacttaca agtacgctca gatccgtctt tggaatgctg
     6481 gaatcgctat tccaataacc tcctgcaaca gtcgtagcaa tgcgttctgg gttcactaga
     6541 agtatgagaa gaccaaggtg ctccccgctc gaaacatctt tgattgttct tgcacatatc
     6601 aggtaattgg atgtttcaga agaatctgtt gacgaaggtg aggaaatctg aagaaaagtc
     6661 catataggct tccctcctgc tttctctgca atctcggcag cttgggtctt tgctgaattc
     6721 cagactaatc tggtaagaga atctgtcata gtaagagggc gtttattaat gtttgatgaa
     6781 tcgaagaaca tagcatcttc aagctctggt gatgcctcgg ccattccctc cataaaataa
     6841 gaaaaaatgg aattccatct agacacatcg taagtttctt ttgatgctgt ataatccagg
     6901 agcagatcgt tcagtattct gttagaatag aactgataag taatcgcttc gatagcatta
     6961 gccagttctg atgctctctg agctcttgct tcgattagac gttctgcata ttcgatatta
     7021 tcagacgcaa tgggattgta taatatcata agaataatga atgctattgc cagaataaga
     7081 acaataaaga aaaacaccaa caatcgagaa aagcgaccag actgaccttc ctttggatgc
     7141 aaaacttgtc ttgagataaa acctaaattg tatgattctc cgccgaaatt cattgcatat
     7201 tagtataaca tgagagtgcg caatgttaag cagcagcttc caggtaactg agcgagactt
     7261 gtagctaaaa atttagaagc atagttaatc gttgatcgca gatatcttta agagcaaaat
     7321 ctttattctc tagctacatt attcaaagaa atgatcatct attgctatct aatcttgatc
     7381 catatatttt actggttttt atttcttgct atcaaattta taccgagcct aaccacagaa
     7441 tcgatgtgac gagcctagga tagagtcttg agccgaggtc tttcccgctc gctgaatcga
     7501 cctgatcggt a
//
LOCUS       DAOOWY010000088         7489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_9864_length_7489_cov_11.947807, whole genome shotgun sequence.
ACCESSION   DAOOWY010000088 DAOOWY010000000
VERSION     DAOOWY010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9864_length_7489_cov_11.947807"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..1976
                     /locus_tag="PK105_06900"
     CDS             465..1976
                     /locus_tag="PK105_06900"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU61478.1"
                     /translation="MSDNSLLVELTGITKRFPGVLALDSVDMNIVSGEVHGLVGENGA
                     GKSTLIKILTGAYTHDEGKIIFDGKQLTQLTPSKAIELGIACIYQELDLIPHMTVVEN
                     IFLGRELYKTKGLKIIDRKRMLEETESLLKNLKLNFDPTKNTCDLGIGHRQMVEICKA
                     VNSNAKLIIMDEPTSSLSEHETEELFEIIAQLKEKGVTVVYISHRLDEIKRICDRITV
                     LRDGKKVADLNINDASIDNIIHLMVGRDIVNKYPKIKSNRGKEALSVQGLERKGILHN
                     ISFKAYTGEILGITGLVGAGRTEMARAIIGADPIDAGVVKIFGEEMNIRSPRDSIKAA
                     IAFLPEDRRNQGLVLIKDVLFNVSLVKLKQYSHRGFLQLKNIHQDVDQLVSELKIRTP
                     SVNTIVAELSGGNQQKVVIAKWLLTKARIFIFDEPTRGIDVGAKVEVYNLINELIKND
                     AAIIIISSEMDEVMEMADRIIVMHEGEIMGEFAHEEATQEKILYLASGIKISA"
     gene            2003..2995
                     /locus_tag="PK105_06905"
     CDS             2003..2995
                     /locus_tag="PK105_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61479.1"
                     /translation="MSQVKLSSLTIKQSKTRGSLYNLGVLLSLVLLIIIMSILSPRFL
                     TISNLSNVASQASVSAIVSVGMFLAILTSGIDLSVGSVLALSTMVMGIVSVKLNVNPY
                     LSILICLGTGALLGLINGLLLTKLHLPHPFISTLGMQKMARGIALVITAASPISMFPA
                     PIQFLGNNYIGRLPISIVLVVMLYICVHLFLTRTATGRYIYAVGGNKEASRLSGIKVD
                     SVLNLVYMMSGFTAALAGIVLVGRVNAAYPLAGLDYETDAIAAVIIGGASFFGGIGSI
                     GNTIVGVLIIAVLRNGLNLLNVDSNYQTFAIGAVIIIAVAIDVLRQRVMSGSKK"
     gene            3103..4056
                     /locus_tag="PK105_06910"
     CDS             3103..4056
                     /locus_tag="PK105_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU61480.1"
                     /translation="MKKMKRFVSIILMVAFVFAISAPAFAADKRLKVGVVLKTLSSQY
                     WKIVAAGAKTAAQNNNVDLILLGPPTEDAIEQQINMVQDVLSQNIDVLVFSPSQPAAS
                     VNVLLQAKAKKIPVILVDTGMPEGFNDYASFIGTDNLAAGKAGGKALASQLKPGSKVL
                     LIDGAPGNPACNDRIKGAEEVLKASGMIIAAKQPAYSDREKGYTVTQNVLQANPDING
                     VFSANDEMALGALRAMQQAGKRVPIIGVDATEDALKSILAGEMYGSVAQGNYDMGLLA
                     IEKAIELKAGKTIEKRIDSGATLITKENAQDLLDFRMSIKD"
     gene            4177..5196
                     /locus_tag="PK105_06915"
     CDS             4177..5196
                     /locus_tag="PK105_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU61481.1"
                     /translation="MSSILRIGIVGLGRLGKRHAENLSRKVQGASLVAAASPVDLERE
                     WAEKNFGIATYESLDELLDHSDAEIIWLVTPTSLHADQCIQVLEAGKHVFCEKPLALD
                     VESCDRVIEIAREHPKQRIMIGFMRRFDPAYSQVKREIENYGLGRIFRIHSESHDPID
                     PDGFFLKFAPTSGGIFLDCAIHDIDLTRWMLGGLEPKRVTAYGTRVMYPGLESCNDVD
                     TAVATVEFEKDVLATFHVSRTSHKGYQAIMTVTGTEGAINCGCGLSELPLLREKHGET
                     RIQGQTDFFSRFSDAFLYEANAFVASISSGVSVGATLEDAREATRLAIAMRESLEKGT
                     SIKLD"
     gene            5215..6252
                     /locus_tag="PK105_06920"
     CDS             5215..6252
                     /locus_tag="PK105_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU61482.1"
                     /translation="MRTNKRIRIGVIGVGKMGEQHIKNISYLCPGAILVALHDANQDR
                     AKEIAALYGFPQVFDDPDELIASPIVDAVLITAPDKVHADLILACLRERKPVLCEKPL
                     ASDLKDAKLVLDTEAELNQLLVSLGFQRRFDPAHLAVKEAALEGVIGRPILWKGIHRN
                     SHAPYATNPFFLLTNTAGHDIDSARWLLGSEVKEVYARALRSRDDFPPDSRDLFLLEM
                     IMNDERLASAEIYMSADYGYEVSAELVGQNGVLTTARPDLLIVRAQSKRYAPIAQDWT
                     SPFQEAYAAELNAWIESLAGGRIFPGANTWDGYVAIAVAEAANESLIKGTSYPVELPR
                     KPGLYRLDSID"
     gene            6265..7407
                     /locus_tag="PK105_06925"
     CDS             6265..7407
                     /locus_tag="PK105_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU61483.1"
                     /translation="MKKLHYGMVGGGKGAFIGNIHRQAIALDNLARLTAGSFSHDFSN
                     TLEIGMDLEIPSDRLYPTYLEMAQKEAARKDRIDFVSIVTPNYLHFPIAKAFLNAGIN
                     VVCDKPLALRESEARELDELTIKNGLLFAVTYAYTGYPLVKQAREMVKRGDLGDIRFI
                     NAEYPQDWLLKQFESDGHKLKSWRANPSVAGISNCVGDIGTHIEYLVSYITGLKIKSL
                     CARLDTFMEGHELDDNATILLEFEGDAKGVYWTSQTAIGYDNGLKIRIFGSKGSLRWS
                     QEDPNYLVVSKLNSPTEIISRGRDDLYPKAQDYSRIPAGHPEGYIEAFANIYRAFTNA
                     LLKKSLGDIVTEEELDFPNARDGANGVRFVEKCVESSKKGAIWVEF"
BASE COUNT         2221 a         1476 c         1729 g         2063 t
ORIGIN      
        1 gggccttgaa tatctccgag acccctgcga tatccatgat cgaatttcga agcttttcag
       61 cctccgcccc acaggttatg ctgtattggc tgcatagatc atcggtcata atcgaaccct
      121 caacagatcc cattgaaaac tgaatttacg attgctcact taagcaactg tttaagtata
      181 ttgtaatgca tataacagac ttgatcaaga tatagagaga ttattttttt cagagcctca
      241 ttcgcaagga taatgtgcac ctttcatatg gcagaatcgt tttgacgtga tgatttacat
      301 aattatttga caaattggca aagtggtaca ataataagct ttagccgata tgaaaacctt
      361 ttcagaaaag gttttcatat cgatgaatct cgaataggct aagatcttat gttgttgctt
      421 atacaatagg ttttgagact ggatagccta aaataggaga ttttatgagc gacaatagct
      481 tattggttga actcactgga attaccaagc gattccctgg ggtcttggct cttgattctg
      541 ttgatatgaa cattgtgtct ggcgaagtcc atggcttggt aggtgagaat ggagctggta
      601 aatcaacatt gataaagata cttactggag catacacgca tgatgaagga aagataatat
      661 tcgatggaaa acagttgaca caattgactc catctaaagc aatagaactg ggaatagcct
      721 gcatatacca ggaacttgat cttataccgc acatgaccgt cgtagagaat attttccttg
      781 gtcgtgaatt atataagaca aagggcttaa agattattga ccgcaagcgc atgctagaag
      841 agacagaatc tctgcttaag aatctcaaac tgaatttcga tccaacaaag aatacctgtg
      901 atcttggcat cggacatcgc caaatggttg aaatttgtaa ggccgtcaac tccaatgcaa
      961 aactaataat catggatgaa cccacgtcga gcttaagtga gcatgaaact gaagaactct
     1021 tcgagattat tgctcaacta aaggaaaaag gagtaactgt tgtttatata tcgcaccgac
     1081 tagatgagat aaagcgcata tgtgatcgca taactgttct gcgcgatggc aagaaagttg
     1141 cagatttgaa tataaacgat gcttcaatcg ataatatcat tcatctaatg gttggccggg
     1201 atattgtcaa taaatatcca aaaataaaga gcaatcgtgg taaagaagcc ctttcggttc
     1261 aaggtctcga gagaaagggc atattgcaca atatttcctt taaggcttat acaggcgaaa
     1321 tcctgggcat tactggatta gtgggagctg ggcgtacaga gatggctcgg gctatcattg
     1381 gcgcagaccc aatcgatgcc ggggttgtaa agatatttgg agaggaaatg aatattcggt
     1441 ctccaaggga ttcaataaag gctgccattg ctttcttgcc agaagaccgc aggaatcaag
     1501 gccttgttct cataaaagat gtattattca atgtatctct cgtaaaatta aagcaatact
     1561 cgcacagagg atttttgcaa ttaaagaata ttcatcaaga tgtagatcag ctagtttctg
     1621 agctgaaaat tcgcacgccc agcgtcaata caattgtagc tgaactcagt ggaggaaatc
     1681 agcaaaaagt agtcattgca aaatggcttc taaccaaggc taggatcttt atatttgatg
     1741 aacccacacg aggcattgat gtcggggcca aagtggaagt ctacaatttg ataaatgagc
     1801 taataaaaaa tgatgccgcc ataattatta taagctcgga aatggatgaa gttatggaaa
     1861 tggcagaccg cattattgta atgcatgaag gagaaataat gggggaattt gcccacgagg
     1921 aagcaaccca ggaaaaaatt ctatatctgg catctggaat caaaatatca gcataaattt
     1981 actctattga aggagcagct ctatgtccca agttaaattg agttcactca caataaaaca
     2041 aagtaaaaca aggggtagcc tctataattt aggggttctt ctgagtctgg ttttgctgat
     2101 cataatcatg tctatattat cacccaggtt cttgacaatt agtaatctat ccaatgtggc
     2161 ttctcaagct tctgttagtg ccattgtgtc tgtaggcatg tttttggcaa ttcttacatc
     2221 cggcatagat ctttcggttg gctctgttct tgctctttca acaatggtaa tgggaattgt
     2281 cagcgttaaa ttgaacgtaa atccctatct ttcgatcttg atatgtctgg gaacgggagc
     2341 tctattggga ctgatcaatg gactgcttct tacaaaactg catctgcctc atcccttcat
     2401 atcgactctt ggtatgcaga agatggccag gggcatagcg cttgttatca ccgcagcatc
     2461 gccaatcagc atgtttccgg cgccaataca attcttgggt aacaattata taggaagact
     2521 tccaatcagc attgttctgg ttgtgatgct ctatatatgt gtacatcttt ttcttacaag
     2581 aacagcaact ggtcgctata tatatgccgt aggcggtaat aaggaagcca gcagattgag
     2641 tggtatcaag gtggattctg ttcttaacct ggtctatatg atgagcggct tcaccgctgc
     2701 actggcgggg atagtcttag ttggacgagt caacgccgct tatccattgg caggtctcga
     2761 ttatgaaaca gatgcaatcg ccgctgtaat aattggaggg gcaagtttct ttggtggtat
     2821 tggatccatc ggaaatacaa tcgttggcgt cctgataatt gcggtgctga gaaatggtct
     2881 caatttgctc aatgtcgact caaattacca aacatttgcc attggtgcgg tcataattat
     2941 tgctgttgca attgacgttc tacgacaacg agttatgagc ggcagcaaga aatagtcatc
     3001 taagcaaatc ctaatctaaa tgcaacgaaa aggaggtgat aaacgagaaa gaataaacaa
     3061 gaaagtcgct cgaactcaaa ttttatttag gaggtttcag agatgaaaaa gatgaaaagg
     3121 tttgtttcga ttattctgat ggtagcattt gtctttgcaa taagtgcacc agcattcgcc
     3181 gctgacaaac gactgaaagt gggcgtcgta cttaaaacgc tcagcagcca atactggaaa
     3241 atcgttgctg ccggcgccaa gacggctgct cagaataata atgtggatct cattcttctt
     3301 ggaccaccaa ccgaagatgc cattgaacaa cagattaata tggttcagga tgttctatcg
     3361 cagaatattg atgtcttagt attttctcca tctcagcctg ccgcatccgt caatgtgcta
     3421 ttgcaggcaa aggctaagaa aatccctgtt atccttgtcg acacaggtat gccagaaggc
     3481 ttcaatgatt atgcaagttt catcggtact gacaacttgg ccgctggaaa agctggtgga
     3541 aaagcccttg catctcaact caaaccaggc agcaaagtcc tgcttataga tggggctccc
     3601 ggcaatcccg catgcaacga tcgtatcaaa ggggctgagg aagtgctcaa ggcctctgga
     3661 atgattatcg ctgccaaaca gccggcatat agcgatcgag agaagggtta tacggtaacc
     3721 caaaatgttt tgcaagccaa tcccgacatc aatggagtgt tttctgctaa tgatgaaatg
     3781 gctctcggtg cccttcgtgc aatgcagcaa gctggcaaac gtgtcccgat tattggtgtc
     3841 gatgctacag aagatgctct gaaatctatt cttgcgggag aaatgtatgg ttccgtagcc
     3901 caggggaatt acgatatggg gctgctcgca attgaaaaag caatagagct taaagcgggt
     3961 aagacgattg aaaagcgtat cgattccggt gctacgctga taaccaaaga aaatgcacag
     4021 gacctattgg atttccgcat gtccattaaa gattagtagc aatatctcta tatacggata
     4081 ggtcaaacac ctgcaggagg atgtatcttc ttgcaggtgt tcctaatagg tttcgagata
     4141 tcggcaattt gcatgacatt ttgtacggag gtaatagtgt cgagtatcct gcggattggt
     4201 attgttggat tagggcgtct tggcaaacga catgctgaaa atctctctag gaaagtccag
     4261 ggagcttccc ttgtcgctgc cgcaagccct gttgatttag aacgggaatg ggctgaaaag
     4321 aattttggca ttgcaactta tgaaagtcta gatgagctct tagaccatag cgatgccgaa
     4381 ataatctggc ttgttacccc aacttctttg catgccgatc aatgcattca agttcttgaa
     4441 gctggaaagc atgttttttg tgagaaacca ttggcactcg atgttgaatc ctgcgatcgg
     4501 gttattgaaa tagcaagaga gcacccaaaa cagagaatca tgataggatt catgcgtcga
     4561 tttgatcctg cgtacagcca ggtcaaaaga gaaattgaga actatggact tggaagaata
     4621 ttcagaattc atagtgaatc ccatgaccca atagatcctg atggtttttt cttgaaattt
     4681 gcacctactt ctggtggtat ttttcttgat tgcgctatcc atgatataga tctcactcgc
     4741 tggatgctcg gaggtcttga gccaaagcgt gttaccgcat atggaacacg agtaatgtat
     4801 ccggggctcg aaagctgtaa tgatgtcgat accgccgtcg ctactgtcga atttgaaaaa
     4861 gatgtattag cgacatttca tgtttctcgt acatcgcata aaggatacca agcaataatg
     4921 acggtaacag gaaccgaagg agcaataaat tgcggttgtg gccttagcga gctccctctg
     4981 ttacgcgaaa agcatggaga gacaagaatc caaggccaga cagatttctt ttcgcgattc
     5041 agcgatgcct ttttatatga agcaaatgca tttgtagctt ctatttcatc aggagtatca
     5101 gttggtgcta cacttgagga tgcacgagag gcaactcgcc tagccattgc gatgcgagaa
     5161 tcattggaaa agggaacatc cataaagctc gattgaataa aaaggggaaa caacatgagg
     5221 accaataaga gaattaggat cggtgtcatc ggagttggca aaatgggaga acagcatatc
     5281 aagaatattt cctatctctg ccccggagct attcttgtcg ctctccatga tgccaatcaa
     5341 gatcgggcca aggagattgc agccctctat ggatttcctc aagtctttga tgatccagat
     5401 gaactaattg catcgccaat agtagatgcc gttcttatta ctgcacccga caaggtccat
     5461 gcagatctca tactcgcttg tttacgagaa aggaagcctg ttctttgcga aaagcccctg
     5521 gcttccgatc tgaaagatgc aaaattggtc ctagataccg aagcggaatt aaatcagctt
     5581 cttgtctccc ttggcttcca gcgacgcttt gatcctgctc acttagcagt gaaagaagcg
     5641 gcattggagg gtgttattgg gagacctata ttatggaaag gaattcatag gaattcacac
     5701 gctccgtatg ctacaaatcc tttctttctt ctcacgaata ctgcaggtca tgatatcgat
     5761 tcggcgagat ggctattggg cagcgaagtc aaagaagttt atgccagagc tctgcgctcc
     5821 cgcgatgatt ttccgcccga ttcgcgtgac cttttccttc ttgaaatgat tatgaatgat
     5881 gagcgcctgg cttctgcaga gatctatatg tctgctgatt atggctatga agtttctgct
     5941 gagcttgtag ggcagaatgg cgtgcttacc accgcacggc cagatcttct tatagttcgc
     6001 gcacaatcca agcgatacgc gccgattgca caagactgga cttctccgtt ccaggaagct
     6061 tacgctgccg aactaaatgc atggattgaa tctcttgcag gtggtcgtat ctttcctggt
     6121 gcgaacacgt gggatggata tgtagcaatt gcggttgcgg aagctgccaa tgaatccctt
     6181 atcaaaggaa cttcttatcc tgtcgagctt cccaggaagc ccgggctcta tcgactagat
     6241 tctatcgact gatggagtaa aagcatgaaa aaattgcatt atggaatggt gggcggcgga
     6301 aaaggcgcat tcattggaaa tatacataga caagctattg cattggacaa tcttgcaaga
     6361 cttacagcgg ggagtttttc gcatgatttt agcaatacgc ttgaaatagg aatggactta
     6421 gaaattccca gcgatcgact ataccccaca tatctggaga tggctcaaaa agaagccgcg
     6481 cgtaaggatc gtatagactt tgtttcaata gtcacaccaa attaccttca ctttcctatt
     6541 gcaaaagcat tcctcaatgc tggaattaat gtggtttgtg ataagccttt ggcattaaga
     6601 gaatccgaag caagagagct tgatgaatta acaataaaaa acgggttgct ttttgcagtt
     6661 acctatgcat atacaggcta tcctttggta aaacaagcta gggaaatggt caaacgaggc
     6721 gaccttggag atatcagatt tataaatgcc gaatatcccc aagattggct gctaaagcaa
     6781 ttcgaatccg atggacataa gcttaaatca tggcgagcca atccttccgt agctggaata
     6841 tccaattgtg ttggagacat tggcacgcat atagaatacc ttgttagcta tataacaggc
     6901 ttaaaaatca aatctctctg tgcgcgactt gataccttta tggaaggtca tgaactggat
     6961 gacaatgcaa ctatcctgct tgagtttgag ggagatgcta agggcgtata ttggactagc
     7021 caaactgcta ttggctatga taatggattg aaaataagaa tttttggttc aaaaggatca
     7081 ttaaggtggt cacaagagga tccaaattat cttgttgttt ctaaactaaa tagccccaca
     7141 gaaattatct ctcgaggtcg agatgatctt tacccaaaag cgcaagatta ttcgcgaatt
     7201 ccagctggcc atcctgaggg ttatatcgaa gcctttgcga atatttatag agcattcact
     7261 aatgctctgt tgaaaaaaag ccttggagat attgttaccg aggaggaact tgattttcca
     7321 aatgcaagag acggagccaa tggagtacgt tttgtagaaa aatgtgtcga gagttccaaa
     7381 aagggagcaa tttgggttga gttctgaccg aagggagaaa aatggaaaat gattatgatc
     7441 aataccaata gatttgcgct gaaccgcatt gctgcgccgg cgctagggc
//
LOCUS       DAOOWY010000089         7488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_9868_length_7488_cov_8.589399, whole genome shotgun sequence.
ACCESSION   DAOOWY010000089 DAOOWY010000000
VERSION     DAOOWY010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9868_length_7488_cov_8.589399"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PK105_06930"
     CDS             <1..330
                     /locus_tag="PK105_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOU61484.1"
                     /translation="IKEHPRTGYEILREIDFPWPVAEIVLQHHERIDGSGYPQGLKGE
                     DILFASRIIGVADVVEAMTYHRPYREALGKDAALDEIRQNKGILYDPLVVETCVKVFM
                     EKGLTLD"
     gene            complement(419..>1178)
                     /locus_tag="PK105_06935"
     CDS             complement(419..>1178)
                     /locus_tag="PK105_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU61485.1"
                     /translation="RVQILQYGAELIEVDGTYDLAFEESLEYSRSSGMLSRNTGYNPL
                     TIEGKKTASFEIAKDLAKAEDSARKQGINTNLSQDDELQVSGFPAYLAPDHVFVPTGD
                     GVILSGIIRGFEDLLRLGWINRMPIVWAAQAEGSSAIARALATGSFEARPSHTIADSI
                     AVDVPRNGYFTLYKLKKHGGRAVVVSDEEILKAQTYVARLSGLFAEPSSACAFAGFMK
                     VRSELDPKARIVVMLTGNGLKDITSAAKAVGLRT"
     assembly_gap    1179..1188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1189..1638)
                     /locus_tag="PK105_06940"
     CDS             complement(<1189..1638)
                     /locus_tag="PK105_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU61486.1"
                     /translation="MKWFYSCPSCGRTYPIEPGRYLCDSCSEAQKKNEPLRGVLECSW
                     EGDNPSSGSIPLPVEECFFPSIPVGQTPLWAPERLRTELEMPNLYLKDDTCNPSGSYK
                     DRASWLVAAFARKFGIKEIVLASTGNAASSMACIGAASGIAIKVYVPK"
     gene            complement(1666..2877)
                     /locus_tag="PK105_06945"
     CDS             complement(1666..2877)
                     /locus_tag="PK105_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOU61487.1"
                     /translation="METTKILEKSREYREYTAQNLSKIIKVPAFSTTEKDRIQLLKKL
                     CEEAGMEDLWIDGLGSLLGRVGKGPKKLVFDAHIDTVGIGDESQWKTPPFSGLIKDGL
                     VYGRGASDQLGGAASMITAARILRELAYHGDYEIWFSFTTIEEDCDGMCWKYLIEEEK
                     FIPDFVVSTEPTSCRLYRGHRGRMEIQVNIKGISCHGSAPERGDSAAYKAARAALAIE
                     KLNAELLPDDDHFLGKGTITVSQMDVHGPSQCAVPDQAMLYCDRRLTWGETDKSAISQ
                     VEDALKEAGVDDFTVYMPEYRKPAWTGTEYHQELYFPTWKIPENHILVQSGIDAYTAL
                     FGEKPVVDKWTFSTNAVAICGRHNIPVIGFGPGDEDQAHAPNEVTRVDDLEIASAFYI
                     ALPYALSVLGA"
     gene            complement(2881..4239)
                     /gene="ssnA"
                     /locus_tag="PK105_06950"
     CDS             complement(2881..4239)
                     /gene="ssnA"
                     /locus_tag="PK105_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOU61488.1"
                     /translation="MILFRNVRIMELDPPSVSEPMDVAVFEPEENAHGGTIAEIGGSL
                     QTKYPKAKIAGEGGFLSPGLVCGHTHLYSALSRGMLVDIAPSKDFAQLLKNLWWRLDR
                     AIDPEILRASALAGCSDALKAGVTSLVDHHAGPECIADSLSIIRKAYEDTGLRGILCY
                     ETTDRNGTDEARAGIAENVRFAKEVDNIRASGQKPLVDAAIGAHASFTLSNETLEALA
                     EAVQETGRGIHIHLAEDKFDAVDARYRFGKDPVERLEAVGALNSRGIVGHGVWLTPSE
                     IEIMNVRDVFLAHNARSNMNNAVGYNALLPTFKNVVLGTDGMGADMLEEFKFAVFRHR
                     ESQGPWWPGDFLKALHRGNLLIERYFSQDFDPTSPGFGTIKQGAPADLVLWDYDPPTP
                     LVGANIAGHLAFGMSSRSARTVMIAGSIRILDHRPMYDDMAIEADARCQAARLWKRME
                     ER"
     gene            complement(4236..7451)
                     /locus_tag="PK105_06955"
     CDS             complement(4236..7451)
                     /locus_tag="PK105_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU61489.1"
                     /translation="MSKIMKTTALPALFERIAGEYTTKRSIFEIPESTWLDLFEQEAE
                     SAGMPIMASTISIPLGPAAGPHTQIAPNLIAAYLSGARVFELKTVQENDHLDIDKPCI
                     DALDEGYNVEWSTELSLEEARIEYINAWLVINLFARIWSHKPSDFLFNMSVGYTLEGL
                     KSAKMEAFIEGMRRPETGSYWERALEELKSFVESPLFMQAFGSQALEKARSIAEHMPV
                     RPVHSVTLSTMHGCPPEEIERIGRYLIEEKGFDTYIKLNPTLLGYEDTRTILDRLGWK
                     DIVLKSESFEHDLQFDMAISLIHTLSEVAFNKGRRFGIKLSNTLANINNGDYLPGFER
                     YLSGRALFPITINLAAKLAHALPDFGARFSYCGGVSAFNAADLIRAGMGPLTIATDLL
                     KPGGYQRLGHMVKDVLSSLPFAPEKPDLTALDTLAEGVFSKPYYRKDWKEGTASIEGP
                     LPLFDCFAAPCIEACPVGQKVPAYVAATGAGNANRGLSIVTSDNPFPTITGILCDHIC
                     QEHCSRVDYEGAVRIRDMKLSTVRAAVPESENEVRPAWPRKAIGNTAIIGAGPAGLTC
                     AWYLAQHGQKAVIFEKLPIAGGVPSTVIPSFRISREDIASDIDKLEKLSVEFRFGTEI
                     STIEACEALKRDEFDSIVIAHGAYHARNLVLDGDGVKVMHALDFLRSYMEKGLEHFSG
                     VKTVLVVGGGNTACDSVRLASRIPGIEHVYWSYRRSRKEMPADKEELANAIAEATALT
                     VGCDDWASFSSQDLSFLRNSSFEENNQESILLELTLPEALEPGMVRLRRMKPGEKDES
                     GRPSPVPTDETFLLPCDLIITAVGEAPDPTLFSNLGVEVSKNGLPIVDEETMESSIPG
                     IYICGDARRGPSSIIASEADGRAAAYAILKKREIEVAKNDYRAPLWDSGSRSNRGKMI
                     EPLSPDDPSFAEIEAERCLACDSACLRCVEVCPNRANIIIETGRLFDQPAQILHIDRL
                     CNECGNCARFCLWKGEPYKHKPTLFDTLDDLRVSNNAGFAFFGNVKRPSLVFRAKVGD
                     AIAELPFSAWNGVISLSVHRAILALARVIWSEHRYLVEVHE"
BASE COUNT         1881 a         1881 c         1766 g         1950 t
ORIGIN      
        1 atcaaagaac atccccgaac aggttatgaa atactgcgag agatagattt cccatggcct
       61 gttgctgaaa ttgtcctgca acaccatgag aggatagatg gatctggcta tccacagggg
      121 ctcaaggggg aggacatctt gtttgcaagc agaatcatag gcgttgccga tgtcgtcgaa
      181 gctatgactt atcaccgccc gtatagagaa gcacttggaa aggatgcagc gctcgatgag
      241 atcaggcaaa acaaagggat cttgtatgat cctcttgtcg tggaaacctg cgttaaggtt
      301 tttatggaaa aaggcttaac actggattga tttctcctga tcaccactat tcgtatccaa
      361 atgccggtga taggtgcatc tcgaccatgc acacataaca actatttgca cagacaaatt
      421 atgtgcggag gccgacagct ttcgctgcgg atgttatatc tttaagccca ttgcctgtga
      481 gcatcacgac aatgcgagcc tttggatcga gctctgagcg caccttcata aatcccgcaa
      541 aggcgcaggc actcgaaggc tctgcgaaga ggccagatag tcttgcgaca taggtctgag
      601 ccttaaggat ttcctcatcc gagacaacaa cggcccgacc accatgcttc tttagcttgt
      661 atagagtaaa ataaccattt cgcggtacat cgaccgcaat cgaatcggca atagtgtgcg
      721 atggacgcgc ttcgaaactg cccgttgcaa gggctcgggc aattgcgctc gagccttcgg
      781 cctgagctgc ccatactatt ggcattcgat tgatccaacc caggcgcagg aggtcttcga
      841 aaccacggat aattccagat agaattacgc catctcccgt gggtacaaag acatggtcag
      901 gtgcaagata agccggaaag ccggaaactt gaagctcatc atcttggctt agattcgtgt
      961 tgataccctg ttttcttgcg ctatcttcag cctttgcgag gtctttggct atttcgaagc
     1021 tggcggtttt cttgccctct atagtcagtg gattatatcc agtattgcgc gaaagcatac
     1081 cgcttgatct ggagtattcg agcgactctt caaaagcgag gtcgtatgtg ccatcgacct
     1141 cgataagctc tgcgccatac tgcagaatct ggactcgcnn nnnnnnnnct ttggaacata
     1201 gacttttatc gctatgccag atgcggctcc aatgcaggcc atactcgaag cggcatttcc
     1261 ggtcgaagca aggacaattt cctttatgcc gaatttacgc gcaaaggctg ccacaagcca
     1321 gctagctcta tctttatacg agccagaggg attacaggta tcatccttga ggtagagatt
     1381 tggcatttca agctcagtcc gcaatcgctc gggagcccat aatggcgttt ggcctactgg
     1441 aattgatggg aaaaagcatt cttccacggg caggggtatg gagccactag aaggattatc
     1501 accttcccaa gagcattcca gtacgcctcg caagggttca ttttttttct gtgcttctga
     1561 acagctgtcg catagataac gcccgggttc gattggatat gtccttccgc atgaaggaca
     1621 gctataaaac catttcatgt atctactgcc tttaccattg gttttttagg cacctagaac
     1681 cgataacgca tagggcaggg caatatagaa ggccgaggct atctcaagat cgtccacacg
     1741 cgttacctca ttgggagcat gggcttgatc ctcgtctcct gggccgaatc cgatcaccgg
     1801 gatattatgg cgcccacaga tcgcaacggc atttgtcgag aaagtccatt tatcgacgac
     1861 aggcttttcc ccaaaaagcg cggtatatgc gtcgatacca gattgcacaa ggatatgatt
     1921 ctctggaatc ttccaagtgg ggaaatagag ttcctgatga tactccgttc cggtccaggc
     1981 tggctttctg tattcaggca tatagacagt aaagtcatct actccagcct ctttcagagc
     2041 atcctctacc tggcttatag cgctcttgtc agtctctccc caagttagtc gacgatcgca
     2101 atatagcatg gcttgatcgg gcacagcgca ttgacttgga ccatgtacat ccatctggct
     2161 tacagtgatt gtgccctttc caaggaaatg atcatcatcg ggtaagagtt cggcattgag
     2221 tttttcgatt gccagggcag ccctggccgc cttgtatgcg gcactgtcgc cgcgctccgg
     2281 agccgagcca tggcaagata ttcctttaat gttgacctgg atttccatgc gtccacggtg
     2341 ccctcgatat aatctgcagg aggttggctc ggtcgataca acaaaatcgg gaatgaattt
     2401 ctcttcttcg atgagatatt tccagcacat tccatcgcaa tcttcttcga tcgtagtgaa
     2461 tgaaaaccag atttcatagt ccccatggta agccaattct cttaggatgc gcgcagcagt
     2521 gatcatcgaa gcggctccgc ctagctgatc agaggctcct ctcccgtata caaggccatc
     2581 tttgatcagt ccagaaaaag gaggtgtctt ccattgcgat tcatcgccta tgccaactgt
     2641 gtcgatatgt gcatcgaata caagcttttt cgggcctttt cccacgcgac caagaagaga
     2701 tccaagtccg tcgatccaga gatcctccat gccggcctct tcgcagagct ttttcaatag
     2761 ctgaatacga tctttttcgg tggtggaaaa agccggcacc ttgatgattt tgctcagatt
     2821 ctgggcagtg tactcgcgat attcacgaga tttttccaaa atcttggttg tttccattta
     2881 ttacctctct tccattcttt tccagagcct tgcggcttgg cagcgtgcat ctgcttcgat
     2941 agccatgtca tcatacattg gtctatgatc gagaatccgg atcgatcctg caatcatcac
     3001 cgtgcgggct gatctactgg acattccaaa cgcgaggtgt ccggcgatat tcgctccaac
     3061 aagcggagta gggggatcat aatcccagag cacaagatca gctggagcgc cttgctttat
     3121 agtaccaaat ccaggagaag tcggatcgaa gtcctgcgag aaatatcgct ctatgagaag
     3181 atttcctcta tgcaatgcct ttagaaagtc accaggccac catgggcctt ggctttcgcg
     3241 atgccggaaa accgcgaatt tgaattcctc gagcatatcc gcgcccatgc cgtcggttcc
     3301 aagcacgaca ttcttgaatg taggtagcag agcgttatag ccgactgcat tgttcatgtt
     3361 ggaacgagca ttgtgggcaa gaaatacatc tctgacattc attatctcga tttcggaagg
     3421 cgtgagccat acgccatggc cgacaatgcc gcgagagttg agggctccta cagcctcaag
     3481 gcgctcgaca ggatccttac caaagcgata tctggcatca acggcatcga acttgtcttc
     3541 tgctaaatgg atatgaatac ctcggccagt ctcctgtacc gcttcagcaa gcgcttctaa
     3601 agtctcgttg cttagtgtaa agctcgcatg ggcgccgatg gcggcatcga caagaggctt
     3661 ttgcccagat gcgcggatgt tgtctacctc cttggcgaat cttacatttt ccgcgattcc
     3721 agccctcgcc tcgtctgttc cattacggtc agttgtttcg tagcacaata tcccgcgcaa
     3781 acccgtatct tcataggctt ttcgtataat tgaaagacta tcagcaatgc attcaggtcc
     3841 ggcatgatga tccacaaggc ttgtaacacc tgctttgagc gcgtcggaac aacctgcaag
     3901 cgcgctggcg cgcaagattt ccgggtcgat tgcccgatcg agtcgccacc acagattttt
     3961 gagaagctgt gcaaaatcct tagatggtgc aatatctacg agcattccac gcgaaagcgc
     4021 ggagtataga tgagtatgtc cgcatacgag tcctggggaa agaaatcccc cttctcccgc
     4081 tattttagct ttggggtact ttgtttgcag agagccccca atctctgcaa tagtcccccc
     4141 atgtgcattc tcttctggct caaataccgc aacgtccatc ggctcggaaa ccgaaggcgg
     4201 atccagttcc atgattctta cattgcgaaa taagatcatt catgtacctc cacgagatat
     4261 cgatgctccg accaaataac acgggctaag gcaaggattg cgcgatgtac cgatagagaa
     4321 ataacgccat tccaggcgga aaatggtagt tcggcaatag cgtctccgac ttttgcccgg
     4381 aaaaccagcg acggtctttt cacgtttcca aaaaaggcaa aacctgcatt gtttgaaaca
     4441 cggagatcat ctaaggtatc gaaaagagtc ggcttatgct tatatggctc acctttccat
     4501 agacaaaagc gtgcgcagtt accacattca ttgcagaggc ggtcgatatg caggatctgt
     4561 gcgggttgat cgaataatcg tccggtttcg atgattatgt ttgcccggtt gggacagact
     4621 tcgacacatc tcaggcaagc tgaatcgcaa gctaggcaac gttccgcttc tatctctgca
     4681 aatgaagggt catcgggaga tagaggctct atcatcttac cacgatttga acgagaacca
     4741 ctgtcccata aaggagctct atagtcattc ttggctactt ctatttctct cttcttgaga
     4801 atggcatagg ctgccgctct accatccgct tccgaggcaa tgatcgaaga aggcccccta
     4861 cgagcatcgc cgcaaatata gattccggga atcgaagatt ccattgtctc ttcgtctact
     4921 attggaagac catttttcga tacttcgact ccaagatttg aaaagagtgt gggatcagga
     4981 gcttctccca ccgcggtaat aatgaggtcg caaggaagca aaaatgtttc atcagttgga
     5041 accggactag ggcgtccaga ctcatctttc tctcctggtt tcatgcggcg caaccttacc
     5101 atacctggtt ccagtgcttc agggagcgtc aattcgagaa gaatggattc ttgattgttt
     5161 tcttcaaaag atgaatttcg gagaaatgag agatcctgag aagaaaatga agcccaatca
     5221 tcgcaaccaa ctgtgagtgc agtcgcttcg gcgattgcat ttgcaagctc ttctttatct
     5281 gcaggcatct cctttctgct gcgtcgataa gaccagtaga catgttctat accagggatt
     5341 cttgatgcca accgaactga gtcgcatgca gtgttgcctc ctcccacaac gagcacagtt
     5401 ttcactccac taaaatgttc aagccccttt tccatatagg agcggagaaa atcgagtgca
     5461 tgcatgactt ttactccatc tccatccagc accaaattgc gagcatggta tgctccatga
     5521 gcaatgacaa tggaatcgaa ttcatctctt ttgagcgctt cgcaagcttc tattgtagaa
     5581 atctcagtgc cgaatcgaaa ctctacacta agtttttcca gtttatcgat atcggatgca
     5641 atatcttctc gggaaattct gaaagaaggg atgactgtgg aaggtacacc gccagcaata
     5701 ggcagctttt caaagattac ggctttctgc ccatgttggg ctagatacca ggcacaagtg
     5761 agacccgcag gccccgcccc gattatcgcc gtatttccaa tagcttttct tggccacgca
     5821 ggtcgcactt cattttcgga ttctgggact gccgctcgga ccgttgacaa tttcatatcg
     5881 cggattcgca ccgcgccctc ataatcaacg cgggagcaat gttcctgaca gatgtggtcg
     5941 cacagaattc cagtaattgt ggggaatgga ttatccgatg tcacaataga aagacctctg
     6001 tttgcattgc cggctcctgt agcggcgaca taagcaggta ccttttgtcc aacaggacat
     6061 gcttcgatgc aaggcgccgc aaaacaatca aaaagcggca gaggaccttc aatggaagct
     6121 gtgccttctt tccaatcctt tcggtagtaa ggttttgaaa agactccttc ggcgagtgta
     6181 tctagagctg tcaaatccgg tttttcaggc gcaaaaggta ggcttgaaag aacgtctttc
     6241 accatgtgcc ccaagcgctg ataaccgccc ggcttcaaca aatcggttgc aattgtcagt
     6301 gggcccattc ctgctctgat tagatcagcc gcattgaagg ctgagacacc accgcaataa
     6361 gaaaatcgcg ctccaaaatc aggcaaagca tgagcgagtt ttgccgcaag gttgatggtg
     6421 atcgggaaaa gagcccggcc tgagagatat cgctcaaaac caggcagata atctccatta
     6481 ttgatattgg caagagtatt ggagagcttg atgccaaatc tccttccttt attgaacgca
     6541 acttcgctaa gcgtatgtat gagtgatatc gccatatcga attgcagatc atgctcaaaa
     6601 ctctcgctct ttaggacaat atccttccat ccaagcctat caagaattgt tcgtgtatct
     6661 tcatagccta gaagggttgg gttcagcttt atataggtgt cgaagccctt ttcctcaatg
     6721 agatagcgcc ctatgcgctc aatttcctca ggagggcagc catgcatggt cgagagtgtc
     6781 acagaatgta caggccgtac tggcatatgc tctgctatag aacgggcttt ctcgagcgcc
     6841 tgagatccaa aagcctgcat aaagagtgga gattcgacga aagatttgag ctcttctagc
     6901 gcgcgttccc aataagagcc cgtttcaggc ctgcgcatac cctcgataaa agcctccatt
     6961 tttgcgctct tgaggccttc cagagtataa cccacagaca tattgaataa aaaatcgctt
     7021 ggtttatgcg accatattct tgcaaaaaga ttgataacca gccaggcatt tatatattca
     7081 atacgtgctt cttccagaga gagctcagtg gaccattcga cattgtatcc ctcgtcgagc
     7141 gcatcgatac agggtttgtc gatgtcgaga tgatcgttct cctgcaccgt ttttagttcg
     7201 aacactctcg ctcccgaaag atacgcagcg atgaggttgg gagcaatctg tgtatgaggc
     7261 ccagctgctg gtcctagcgg aatgcttata gtggaggcca ttattggcat gcctgcactc
     7321 tctgcttcct gctcgaatag atccagccag gtcgattcag gaatttcgaa tatgctgcgt
     7381 tttgttgtat attcgcccgc aatgcgctca aatagagcgg gcaacgctgt agttttcatt
     7441 atcttgctca tcgatacccc caagtaagaa atgtgccata accaggtt
//
LOCUS       DAOOWY010000090         7279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_10262_length_7279_cov_10.958195, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000090 DAOOWY010000000
VERSION     DAOOWY010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10262_length_7279_cov_10.958195"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1977)
                     /locus_tag="PK105_06960"
     CDS             complement(7..1977)
                     /locus_tag="PK105_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOU61490.1"
                     /translation="MSIIAASNGHFIDDNGRWLILRGVNLGGRSKVPTTPDGRTHFGE
                     GFYNGADVSFVGRPFPLEEADEHFSRLTNWGQKFLRLVVTWEALEHQAPGVYDIEYLN
                     YIEGIVEAAARHGISLFIDPHQDVWSRWTGGDGAPMWTLEAVGFEPSRLHASGAALLH
                     QEMGALYPEMQWFSNHLRLGCATMFTLFFAGNDYAPGVYAQGMPIQDYLQEHYFQAFG
                     LLAKRIASYDNVVGFGSMNEPGEGFIGIRDVRATRSDMLLPGLAPTPWEAMCAGEGLE
                     TSANRVAVKGLRLRSLERVPLGAKSVRAWKEGEVCVWRRVGLWDIENDRPILKQPGWF
                     ASGKSIKAKNRAGKNGAVAGTHSVFNEFYLKPFVERFAEHLANVSGNSKRFMIFVEGY
                     PQGDIPILRPNFFCVNESHWYDSLTLTMKRWTGFLAYNPERQRVVIGSHAVRRYFREA
                     LARIMQHSQEFMSNAPTLLGEFGLPFDLNNRKAYRTGNFKLHEKVLSLYYDALDANLL
                     SATLWNYTADNTHEHGDGWNREDLSVFCSEDGGGRALAGFVRPYAPAVAGDIVLMQFY
                     QRKGRFILEYKPEHSIKAPSELFVPMIQFPRGALISASGATFSEPDYHIAKGTKKASL
                     PAGAPGGSLQSLILKIDAEPDAELCRLVISRL"
     gene            2130..4703
                     /locus_tag="PK105_06965"
     CDS             2130..4703
                     /locus_tag="PK105_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61491.1"
                     /translation="MKKFLSVSFIAMVLLLSATAQVKNGPYADKVIYDVRMDQTIAIK
                     DTAEGKTDVFFTGLDGKTYNGILPSDLEKLSTYAVPSGSWSLLLNNIPNKAPYTFQTK
                     DGRTIFNPLAIQEVRYALNWLIDRKKIVDEILMGAGEPMITAQTPGQPGTYRFNLVPA
                     KLGMTVRGDEKRALSDIDAALRAAANLPENKGKLVKSGQWWTYNKEPITVKFIIRVDD
                     PTGRLLEGRYIADQIEKAGIKVERLEYDRSKASNLAYSSDPADWEWSMYTEGWGAGAT
                     RAWWDVTISQMYSPYYGYMPGGMTEGFWNYENKEIDRIAQKNINGWFLTADEYWSDNI
                     KVQEMALKEAVRIYVCSQIQYYVANKDRFNSRMLYGLGDGLNNWSVRSADVKPNEKNE
                     KILKITQFSARGALFMSAWDPVGVDGFSDVYSAAIIEACTDPSTFESPNAAKDTPLRV
                     KYDLKNVETKIVAGQPGEPPVGQIPVDKAAVIFNSKTKKWESGIEYKDVTGEGTYDYV
                     KNNDLKSYSKLANQQYIYGKWHSGQPITVADIMYATAFAYEWANKDGEDDKYYDAAYA
                     AQYQAYLPVSKGTVLNKDGSFTTYFDFNWPMDKDRVAATGAVSPKAGNPGRQTLVSWE
                     IYEALAKLVAEGSKSGTQYSFSNDPSMTEVDIINLKCVEDIKAKLQDFVAAKYVPDCI
                     KQWVTPEQAVARYNAAIKFIDTYGNAYISNGPFVLSKIDFNTNYVELSAFRDYTYKAD
                     YFPKLFRTTITRIDEVKVPATAARNVDAKIDLQISAVTYPDDTAKPADNKAKVTVTLI
                     KADNTETVYTAKYVKAGSFQAVIPAKDLAALKPGSYTIVVQSVFTTEAPAVQAASLVV
                     F"
     gene            4827..5867
                     /locus_tag="PK105_06970"
     CDS             4827..5867
                     /locus_tag="PK105_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61492.1"
                     /translation="MYKWFAVKRMLKGIFTYVIIIFIMSVLFNTVNEQTMRSSIEEQV
                     RAETMRLKNMQPSALQRFQEQRRQELIRLYRLDRPLMERILFRTWNTITFNFGKSTII
                     KSSRGDREVLAIVGEAIPRSLVLFTVAMAIEIVVGIILGLKKAQKPGGSLDRSTSLIT
                     MIVYGMPTFWLAMILIMFFVYQLRIFPSGGMHTVPPPQGIMYYIDFLWHMTLPLLTLL
                     LIGFWGIAFVVRNIVLSILQEDYIMAARARGIPENKVLFGHTLRTAAPPLITMSVLSL
                     LASIGGAIIFEGIFSWPGLGNLYWIAVQQNDIPVLMGDLAITVGLYQVGLILLDLTYG
                     FMDPRIKVGGKA"
     gene            5864..7273
                     /locus_tag="PK105_06975"
     CDS             5864..7273
                     /locus_tag="PK105_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61493.1"
                     /translation="MKFADFKYGLSEFWSEFKKERSGLVGLAILILSILVVIFEPLIL
                     PWKEANTKWRSIDYWQDNSAGAPPAWTNFFSKQKAAITVRLENPKDEVIEMDGGIKLA
                     TYTFDYDYTADKAPLDVIFHMIGHGDFPVQVAVERPDGQIIELAQRFEQGLSGQDIRI
                     SLDNDGRESVFTFIKSHESEEALKEYSSKSLRTCNIVFNQTREGMATEFKSLKGNYKF
                     IVRALLLVPGYSEVEDPYTVVTGSVSGLLGTDDSKRDIFSGLVAGLKWALIIGLLTSA
                     ISVLIGVMYGIVSAYFGGAVDSVMQFIYQIVNSVPILPVLIVVSAIFKPSIYMLITAM
                     VLFFWTGSVMTVRSMALQIKEETYIEAAKALGAKHSRIIFKHMVPLLIPYSFASMALS
                     VPSAIVYESSVSLLGLGDATIVTWGQILHDAMQGAAILKGIWWWVLPPGLLIAIMGMT
                     FAFLGFSMDKILHPKLKTR"
BASE COUNT         1923 a         1742 c         1753 g         1861 t
ORIGIN      
        1 tttctatcaa agccgcgaga ttaccaagcg gcacaattca gcatccggct cagcatcgat
       61 tttgagtata agagactgaa gggaccctcc aggcgcgcct gcaggtaggc tggctttctt
      121 tgttcctttt gcaatatgat aatcaggctc agagaatgtg gctccagatg cagatatcaa
      181 ggcgccgcga ggaaactgta tcatcggaac aaaaagctca gaaggcgctt tgatagaatg
      241 ttcaggcttg tattcgagga tgaatcggcc ttttctttgg tagaattgca tcaagacaat
      301 gtcacctgca accgctggtg cataggggcg cacaaaacca gcaagcgctc ttccaccacc
      361 gtcttcgctg caaaaaaccg aaaggtcttc tctgttccag ccatcgccat gctcatgagt
      421 gttgtcagcc gtatagttcc aaagcgttgc gcttaagaga tttgcgtcaa gggcatcata
      481 atatagagag agtacctttt catgcagttt aaagtttccg gttcgatatg ctttgcgatt
      541 attcagatcg aatggtaggc caaattcgcc aagaagagtt ggggcattac tcatgaattc
      601 ttgtgaatgc tgcatgatcc gcgccaaggc ctctcggaaa tagcgccgca ccgcatgcga
      661 accaataacg actctttgtc tttcgggatt atatgcaaga aaaccagtcc agcgcttcat
      721 ggtaagcgtc agcgaatcat accaatggct ttcgttgacg cagaaaaagt taggccgaag
      781 tatgggtatg tcgccctgtg gatagccttc tacaaatatc ataaagcgct ttgagttgcc
      841 agatacatta gctaaatgct ccgcgaatcg ctcgacaaat ggcttcaaat aaaattcgtt
      901 gaagactgaa tgagtacctg cgacagcccc attttttccc gctctattct ttgctttaat
      961 tgattttccg gaggcaaacc aacctggctg tttcaatatt ggtctatcgt tttcgatatc
     1021 ccataagccg actctacgcc atacgcagac ttctccttcc ttccaggcac ggacgctctt
     1081 tgcgcccaaa ggaactcttt ccagggagcg caatctcagg ccttttacag ctactctatt
     1141 ggcgctcgtt tctagtcctt ccccggcgca catcgcttcc cagggagttg gcgctagccc
     1201 aggcagaagc atatcacttc gagtagcgcg aacatcgcgg attccgataa atccttcccc
     1261 gggctcattc attgatccaa acccaactac attgtcataa gaagcaattc gcttggcgag
     1321 caggccaaaa gcctggaaat agtgttcctg aaggtaatcc tggattggca ttccttgggc
     1381 gtaaacacca ggagcataat cattccccgc aaagaaaaga gtgaacatcg tagcgcaacc
     1441 aagcctcaag tgattcgaga accattgcat ttcggggtat agcgctccca tctcttgatg
     1501 aagcagtgca gcgccgctcg catgaagcct tgatggctcg aagcccacgg cttcgagcgt
     1561 ccacattgga gctccatctc ctcctgtcca gcgagaccat acatcctgat gtggatctat
     1621 gaagagagat attccatgcc gagcggcggc ttcgactata ccctcaatat aattgagata
     1681 ctcgatatca tagacacctg gtgcttgatg ctcgagcgct tcccatgtca ccacaaggcg
     1741 caagaatttc tgaccccagt ttgttagcct tgaaaaatgc tcgtctgcct cttcgagagg
     1801 aaaaggcctg ccgacaaacg agacatccgc gccattatag aatccttctc caaagtgagt
     1861 tcgcccatcc ggggttgttg gcactttact ccttcccccg aggttcaccc cgcgcagaat
     1921 gagccagcgc ccattatcgt cgataaaatg tccattcgat gcagctatta ttgacattag
     1981 acctttccct tcagtcttac ttgcatgatc aatataagaa tacgcctgtt attacagcgc
     2041 agcaagccgt aaatcatgat tcttctcttt acaactctaa aagctcgttc taaaatggaa
     2101 aaaggcaaac cttaaaaaag gaggtacgca tgaaaaaatt tcttagtgta tcgttcatcg
     2161 ctatggtgct gctgctcagc gcaacagccc aagtgaagaa cggcccgtat gcggacaagg
     2221 ttatctacga tgtgcgaatg gatcagacca ttgccatcaa ggataccgcc gaagggaaaa
     2281 cggatgtctt cttcactggt cttgacggaa agacctataa tggtatcctc ccatctgatc
     2341 tggaaaaact ttctacttat gcggtgccat cggggagctg gtctcttctc ctgaacaaca
     2401 ttccaaacaa agcgccctat acattccaga ccaaagacgg tcggacgatc tttaatcccc
     2461 tagcaattca ggaagtccga tacgcactca attggctcat cgaccgcaaa aagatcgtcg
     2521 atgagatctt gatgggcgca ggcgagccaa tgataacagc ccagacacct ggacagccgg
     2581 gaacatatcg cttcaatctc gtgcctgcta agttaggtat gacggtacgt ggcgatgaga
     2641 aaagagctct gtcagatata gatgccgcat tgagagcggc agctaacctg cccgaaaaca
     2701 agggcaagct cgtaaagtct ggtcagtggt ggacctataa caaggaacct attaccgtca
     2761 aattcataat ccgtgtcgac gatccaacag gtcgacttct cgaaggccga tatattgcgg
     2821 accagatcga gaaagctggg atcaaggtag agcgcctcga atatgatcgc tccaaggcta
     2881 gcaatcttgc ctacagcagc gaccccgcag attgggaatg gtcgatgtac accgaaggct
     2941 ggggtgctgg tgcaactcgg gcttggtggg atgtcaccat cagtcagatg tattcaccct
     3001 actatggcta tatgccaggc gggatgaccg agggcttctg gaactacgag aataaggaaa
     3061 tcgatcgtat tgctcagaag aacataaatg gatggtttct caccgccgat gagtactgga
     3121 gcgacaacat caaggtacaa gaaatggcct tgaaggaagc ggtacgcatc tatgtatgca
     3181 gccagatcca gtactatgtt gccaataagg atcgtttcaa ttcgcggatg ctctatggcc
     3241 taggtgatgg gctcaacaac tggtcagtgc gatctgcgga tgtaaagccc aatgaaaaga
     3301 acgaaaaaat attgaagatc actcagttct cggctcgcgg tgcgctattt atgagtgcat
     3361 gggatcctgt gggtgtcgat ggtttctcgg atgtctactc tgctgccata atcgaggctt
     3421 gtaccgatcc ctccaccttc gaatctccga atgccgccaa ggatacgccg ctacgcgtaa
     3481 aatacgatct caagaatgtc gagaccaaga ttgttgcagg ccagccaggc gagccgcctg
     3541 ttggccagat tcctgtcgac aaagctgcgg taatcttcaa ttctaagaca aagaagtggg
     3601 aaagcggaat cgaatataag gatgtaacag gcgaaggaac atacgattat gtgaagaaca
     3661 atgatctaaa gtcctacagc aaacttgcaa atcagcagta catctatgga aagtggcaca
     3721 gcggtcagcc tataacggta gctgatataa tgtatgcgac tgcctttgcc tacgagtggg
     3781 ctaacaagga tggcgaggac gacaagtact acgatgcagc ttatgcagct caatatcagg
     3841 cctatttgcc tgtttccaaa ggtactgtgc tcaataagga tgggagcttt acgacatatt
     3901 tcgatttcaa ctggccgatg gacaaagatc gtgtagctgc aacaggtgca gtcagcccca
     3961 aggcaggcaa ccccggacgc cagactctag tatcctggga gatctacgaa gctttggcta
     4021 agctcgttgc agaaggctct aaatctggaa cacagtattc attctcaaat gacccttcta
     4081 tgacggaagt ggatatcatc aatttgaaat gcgtcgaaga catcaaagct aagctgcagg
     4141 acttcgttgc tgccaagtat gttcccgact gcatcaagca gtgggttacc ccggagcagg
     4201 ctgttgctcg ctataatgca gccataaaat tcatcgatac ctatgggaat gcttacatct
     4261 ccaatggtcc atttgtcttg agcaaaatcg atttcaatac taactatgtc gagctttccg
     4321 ctttcaggga ttatacatac aaagctgatt atttcccgaa actgttccgc accaccataa
     4381 cgcgaatcga cgaagttaag gtgcctgcga ctgcggcgcg caatgttgac gccaagatcg
     4441 accttcagat atccgccgtg acctatccag acgatactgc caagccagcg gataacaagg
     4501 ccaaagttac cgtaacttta atcaaggcgg acaacacaga aactgtgtat accgcgaagt
     4561 atgtcaaagc aggcagcttc caggcggtga tccctgcgaa agatctcgca gctttgaagc
     4621 caggttccta cactattgtc gtgcagtctg tctttactac agaagcacct gcggttcaag
     4681 ctgcttcgct tgtagtcttc tgatataaat caggggaagg gcgctgtcta aaagaggcgg
     4741 cgccctcctt attttctaaa atgctttttt aatgtaataa tcgaagtgca attctctcaa
     4801 gggatcaaaa ataaataggt ggtcttatgt ataagtggtt tgccgtcaag cgcatgctga
     4861 aaggcatttt tacttatgta ataattattt ttatcatgtc tgtgcttttc aatacggtga
     4921 atgaacagac aatgcgctca agcatcgaag agcaagtgcg agctgagacc atgcgcctca
     4981 agaatatgca gccatctgcc ctccagcgtt ttcaggaaca gcggcgccag gagctgatca
     5041 ggctctatag gctggatcgt ccgctaatgg agcgcattct cttccgcacc tggaatacca
     5101 tcactttcaa ttttggaaag tcgactatca ttaaatcctc tagaggcgat cgagaggtgc
     5161 ttgctatcgt cggagaggcg attccgcgct ctctagtgct ttttacggta gctatggcca
     5221 ttgaaatcgt ggttggcatt attctcgggc ttaaaaaagc tcaaaaacct ggcggttcgc
     5281 tagatagaag cacaagcctc atcaccatga tcgtctatgg catgccgaca ttctggcttg
     5341 cgatgattct cataatgttt tttgtctacc aactaaggat atttccttct gggggcatgc
     5401 atactgtgcc acccccgcaa gggataatgt attatataga ttttctgtgg cacatgacac
     5461 tgcctctctt gaccttgctt ctcataggat tttgggggat agcctttgta gtgaggaata
     5521 ttgtgctgag catcctccag gaggattaca tcatggccgc acgcgcgcgt ggcattccag
     5581 agaacaaagt gctgtttggg cacactttgc gcaccgccgc gcctcctcta attacaatgt
     5641 cagttttgtc tctgcttgcg tcgattgggg gagcaatcat tttcgaaggt attttttcct
     5701 ggccagggtt ggggaatcta tattggatag cggttcagca aaatgacatc cctgttctaa
     5761 tgggagacct tgccatcacc gtgggattat accaggtcgg gcttatattg ctggatctca
     5821 cctatgggtt tatggatccg cgtatcaagg tgggaggcaa ggcatgaaat ttgctgattt
     5881 caagtatgga ctatcggagt tctggtcaga attcaagaaa gagcgttccg gacttgttgg
     5941 ccttgcgata ttgattctct cgattctagt cgtgatcttt gagcctttaa ttttgccatg
     6001 gaaagaagca aatactaagt ggcggagtat agactactgg caggataact cagcgggcgc
     6061 gcctcccgcg tggacaaact tcttctcaaa gcagaaagcc gcaataaccg tccgtctcga
     6121 gaatcccaag gatgaagtaa tcgagatgga tggaggtata aaacttgcca cctacacctt
     6181 cgattatgac tataccgcag acaaagcgcc cctcgatgta atatttcata tgattggaca
     6241 tggggatttt cctgtacaag ttgcggtgga acgacccgat ggacagatca tcgagcttgc
     6301 tcagcgtttc gaacaaggat tgagcggcca ggatatccga atctccttgg ataatgatgg
     6361 gcgcgaatcg gtgttcacct tcatcaagag ccatgaaagc gaagaggctc tcaaagagta
     6421 ttccagcaaa agtctaagaa cctgcaacat agtgttcaat cagacgagag aaggtatggc
     6481 caccgaattc aagtctctca aagggaacta taaatttatt gtgcgagcct tattgctcgt
     6541 tcctggatat tccgaggtcg aggacccata tactgtagta acaggctctg tttcgggatt
     6601 attaggaaca gatgattcaa agcgcgatat cttttccggt ctcgtggctg gactcaaatg
     6661 ggcactgata attgggcttt tgacttctgc tatctcggtg ctgattggag tcatgtatgg
     6721 tatcgtctcg gcatactttg gaggtgccgt agattcggta atgcagttca tctatcagat
     6781 tgtgaattcg gttccaattt tgcctgtact catagtggta tccgctattt tcaagccgag
     6841 catctatatg ttgattactg caatggtgct ctttttctgg acaggctccg tgatgacagt
     6901 cagatctatg gctctccaaa tcaaggagga gacatatatc gaggctgcca aggcgctggg
     6961 tgcaaagcac tccaggatca tatttaagca tatggtgccg cttttgattc catacagctt
     7021 tgcatccatg gccttatctg tgccgagcgc aatagtctat gaatcttcgg tgagcctttt
     7081 aggcctcgga gacgcaacta ttgtcacctg gggtcagatt ctccacgatg caatgcaggg
     7141 agcggctatc ctcaaaggaa tttggtggtg ggtgctgccg cccggactac tgatcgctat
     7201 aatgggcatg acctttgcat tcctgggctt ctcgatggac aagatactcc atccaaaact
     7261 aaagacaagg taacctcga
//
LOCUS       DAOOWY010000091         7189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_10417_length_7189_cov_9.048921, whole genome shotgun sequence.
ACCESSION   DAOOWY010000091 DAOOWY010000000
VERSION     DAOOWY010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10417_length_7189_cov_9.048921"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PK105_06980"
     CDS             <1..1042
                     /locus_tag="PK105_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU61494.1"
                     /translation="DSITPMISVFSPYITRRDMDLVLSRMVEDAVSEDVFNRRFENAV
                     KEQFNLEYVISMRSPYNALQKALQVFGLERGAKIAISALAPAWQKDALEDSGFTPVIL
                     DIDQETFHPKPESISLANPSAIILFDALGKSPSEALLERIDIPIIEDISQTLGMIKVS
                     DEKHLYGHFLIWSLESDSAIATGGGALLCASGKRDAQILRLLDESMPRELKMTDYNAA
                     LGISQMKSYPKMIERRKTIYRRLVGQLLRTRHSYLQQEDSESCPLYAFPIFPETSVKE
                     IIDYAMKHGVESKHAFASCISQRLYESEPEQEAVFPIARSVALRCILFPMHHKLTNQQ
                     VEQIGKVIATLP"
     gene            1052..1906
                     /locus_tag="PK105_06985"
     CDS             1052..1906
                     /locus_tag="PK105_06985"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOU61495.1"
                     /translation="MSESKSTLIFANLNKDDAGHAAQIVQAELEQNGWHTEILAFRGS
                     ISEYPDFDRYSLIVSLGGDGTLLYAARFAGPRGIPILPINLGTLGFIAANRMETWAVT
                     FEHWQKGALDPSRRLMLQSYAYRGKKILHKGIALNDIVVSSESGARMIRMCLSINREC
                     FGYYRADGLIVSTPTGSTAYNLAAGGPALHPEISALIINPICPFTLASRPLVIPASNC
                     IDIEIDETKKAGALLISDGHIVANLEKGDVIRIRKYEKSAFLIVPSGNAFFSALRTKL
                     GWSGDNDA"
     gene            1899..3596
                     /gene="recN"
                     /locus_tag="PK105_06990"
     CDS             1899..3596
                     /gene="recN"
                     /locus_tag="PK105_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOU61496.1"
                     /translation="MLEQLSVRNFAIIESIDIDLSAGMTVFSGETGTGKSLIVDALGF
                     LLGARVDNSIIREGATECSVSGLFSASSNAQVGLWLREKGIEWQINEGILLRRILKQN
                     GRTLAWIQDRQVSRNELLEFTQYLVDIHGQHEHQRLIDSSTHLDMLDAYANLEDELQS
                     YQVLYSEWREATRQLQEVLEQKAKRSQEADYLDFVIRDIASIKPKPNEDNELLTEEKV
                     LSEHEKLFGAVSEASVLLDSGEGGDVLHTLKRIRASIETARSVDIRLGSFSERLSAAY
                     YELEDISESLNKYKAGLRFDPDRLEKIERRLSDFQRLKHKYGPKLDDVLQTYDRAKRR
                     LEALEHAEENAEELEKKEKSLEKEVLNRAYQLSEKRMMAAVSMSSSIREIVKDLGMPE
                     AQVNLRLKRIVDERGGNRATLRGIDEAEFFIAPNPGEPELPLSRIASGGELSRFALAI
                     KAVLASHDEVETLVFDEIDSGIGGQVGVAVGEYLRQLSQYRQVLCVTHLATIAVRADH
                     QGKVDKFVSKGRTGASIRFLSGEDRETEIARMLSGFSESEASRIHAAELLRKAISHN"
     gene            complement(3663..4073)
                     /gene="cdd"
                     /locus_tag="PK105_06995"
     CDS             complement(3663..4073)
                     /gene="cdd"
                     /locus_tag="PK105_06995"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOU61497.1"
                     /translation="MTTEYTYLFKHALKACEQSYAPYSKFRVGAALLCDDDSIITGVN
                     VENRSYGLTICAERNAIAAAIASGKKRFKALVIATPDADYPVSPCGACRQVISEFMHS
                     SAPVIFGNSEDSYISSTVGELLPHDALHELACRG"
     gene            4177..5268
                     /locus_tag="PK105_07000"
     CDS             4177..5268
                     /locus_tag="PK105_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOU61498.1"
                     /translation="MRRRFYGLIIGIAAIIFTLFSIRSTLPEREITSSVFLTFAEALD
                     VSRIPKARSDQIVPFIAESFLGYADPLEELLYVHPFKGRAAIDRLGWITYDRLATDLK
                     LYDLNGGGQQFQAYAYPWFKASWRVLLRADQMGIAVMDKTGLIRWEKEFPTPITAIDA
                     SSLFLAVGTLDGSISVFDPNGWAILSMQNSLQEFQTIYGLALSADSNYLIIVRGISPK
                     KIETYRFTDNSFAKVSESSLMVNTTFQATIAIARDNSHAIMSFGDMIIYYNIKNKYYR
                     ILEAGMAGVELSETQVDFRYFVLGSLKDSLVAILRSDLEGKAKSDVIILNHGLPVHIA
                     PGAFGAIGDEEVIGFVYKDGLELLKGWRP"
     gene            5265..6260
                     /locus_tag="PK105_07005"
     CDS             5265..6260
                     /locus_tag="PK105_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61499.1"
                     /translation="MKGKYPFLISYLWLATLLGALDFKIPEVIDLSGFGTVLGDQAKQ
                     GVSLEYDVRERCYGKIPRSDVQLISLDTTFEAPSSGSIFYSQVEADLPTVFSFPLGGA
                     LAVIHPDRFVSITSGLDPIQVIKSTSRSSKEVQNSFAIKKGDKLTGGDGSGVYPSKNF
                     GVRLFDAKNELWMNTIFLAPWIRDNTSPIMRSAKLIMTGTDKKVFFDIMETNKKEKLI
                     TCPQGNYSLFVDARDIIITSSRYYSAPYCIKVTLDGETVIDASFIAARASGEGISFLG
                     NPPPSRGSIGEGYSGEGYSYHVGQFLILRGKHDLMLQVNDFAGNQTTMRTSITAY"
     gene            6303..7037
                     /locus_tag="PK105_07010"
     CDS             6303..7037
                     /locus_tag="PK105_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOU61500.1"
                     /translation="MSNNRATIADNVTIIKERIWRAAEKAGRSSDKIDLMAVTKFQPI
                     DVIRQAYLAGIRIFGENRVQEASAKFPSLEPELEGASLHMIGTVQKNKINKALTIFDG
                     IQGIDDIETLEAILIRIKAPKRPIKLYLELHTGEISKSGFPNIDELLRGCEVYARYRQ
                     SDPQWMTKALLCGLMTMAPFTQDISLIRRSFKMLSAAKEKIKSSFDFPEFSELSMGMS
                     NDFEIAIEEGSTLVRIGTAIFGERNA"
BASE COUNT         2118 a         1458 c         1707 g         1906 t
ORIGIN      
        1 tgatagtatc acgccgatga tatccgtttt ttccccttat attacccgta gagacatgga
       61 tctcgttctt agcagaatgg tggaggatgc tgtaagcgaa gatgtattta acaggcgctt
      121 tgaaaatgcc gtaaaagagc agtttaatct tgaatatgtg atatcaatga gaagccctta
      181 taacgctctc caaaaggcac ttcaagtttt tggtctggaa agaggtgcaa agatagctat
      241 ttccgcgctt gcaccagcat ggcaaaagga tgccttagag gacagtggat tcacaccggt
      301 aattctggat atcgatcaag aaacatttca tccaaaaccg gaaagcattt ccctggccaa
      361 cccatcggcg attattctgt tcgatgccct tggaaagtcc ccttctgaag ctctgttaga
      421 aagaatcgat atacctatta tagaggacat ttcgcagact ttgggtatga tcaaagtgtc
      481 agatgaaaaa catctatatg ggcactttct gatctggagt ctagagtcag attctgcaat
      541 tgcaactgga ggaggggctc ttctttgtgc gagcggcaag agagacgctc aaatacttcg
      601 attgctggat gaatctatgc caagagagct aaagatgacc gactataatg cggcacttgg
      661 catatctcag atgaaaagct atcctaagat gatagagcga agaaagacaa tctaccggag
      721 attggtaggg caacttttgc ggactcggca ttcatatttg cagcaagagg actctgagtc
      781 ctgtccctta tatgcctttc cgatatttcc tgaaacaagc gttaaagaga ttatagacta
      841 tgcgatgaag catggtgtag aaagcaagca tgcatttgct tcttgcatat cccagagatt
      901 atatgagagc gagcccgaac aagaagcggt attcccaatc gcacggtcgg tagcattgcg
      961 ctgtatttta tttcctatgc atcataaact cacaaaccag caagtagaac agataggcaa
     1021 ggtaattgcc acactaccat aggagattct tatgtcggag agcaaatcta cactaatttt
     1081 cgcaaatttg aacaaagatg atgcaggtca tgcagcacaa attgtgcaag ctgagctgga
     1141 acagaatggc tggcatacag aaatcctggc atttcgaggc tcaatatcgg aataccctga
     1201 ttttgatcgc tatagcctta tcgttagtct tggtggcgat ggcactcttc tatatgctgc
     1261 tagatttgcc ggtcctcgtg gcatacccat acttccaata aacttaggaa ctttaggatt
     1321 tatagccgct aacaggatgg aaacctgggc tgttactttc gaacattggc agaaaggagc
     1381 tctagatcca tcaaggcgct tgatgctgca atcttatgcg tacagaggaa agaagatttt
     1441 gcacaaagga atcgcgctga atgatatagt cgtatcctct gagagcggcg ctagaatgat
     1501 acgcatgtgt ctgtcaataa atagagaatg ctttggttat tacagagccg atggtctcat
     1561 cgtctccaca cctaccgggt ccactgccta taatctggca gcgggcgggc cagccttaca
     1621 tccggagatc agtgccctta taataaaccc aatctgccca tttacgttgg catccaggcc
     1681 tttagttata cccgcttcta attgcataga tattgaaatc gatgagacca agaaagcagg
     1741 agctcttctg atctcggatg gtcatatagt ggcaaatctc gagaagggcg atgtgatccg
     1801 gattcgaaaa tatgagaaga gtgcattctt aattgttcct tcaggcaatg cctttttctc
     1861 tgccctaagg acaaaactgg gttggtcggg agacaacgat gcttgagcag ctttcggtgc
     1921 gtaatttcgc cattattgag agcatagata tagatttatc tgccggaatg acggtgttct
     1981 caggagaaac cggaacaggc aaatcattga tcgtcgatgc tctaggattt ttgcttggcg
     2041 cacgagtcga caattcaata atccgcgaag gagccacaga atgctccgta tcagggcttt
     2101 tctctgcttc gagcaatgct caggtcgggc tctggcttag agaaaaaggc atcgaatggc
     2161 agatcaatga aggaattttg ctgcgtcgaa ttctcaaaca gaatggcaga acactggcat
     2221 ggattcaaga tcgacaggtt tccaggaatg agctcctgga attcactcag taccttgtag
     2281 atatacacgg tcagcatgaa catcaaagac ttattgattc gtctactcat ctggacatgt
     2341 tggatgcata tgctaaccta gaggatgaat tgcaatcgta tcaagtgctg tattcggaat
     2401 ggagagaagc gactcgtcaa ctgcaggaag tactggaaca aaaagcaaag cgctcgcaag
     2461 aagctgatta tctcgatttt gtaattagag acattgcatc gataaagccc aaacccaatg
     2521 aagacaatga gcttttgact gaggaaaagg tactctccga gcacgagaag ctatttggag
     2581 cagtatctga ggcatcagtg cttttggata gcggggaagg tggcgatgtg ctccataccc
     2641 taaaaaggat tcgagcaagt atcgaaactg cgcgctcagt cgacattcgg ttaggatctt
     2701 tctctgaaag gctttccgca gcctactatg agctggaaga catatcagaa tctttgaata
     2761 agtacaaggc tggccttcga tttgatcccg atcgtctgga aaaaatcgaa aggagactat
     2821 ccgattttca acgcttaaag cacaaatatg ggccaaagct cgatgatgtt ctgcagacat
     2881 acgacagggc aaagagacga ctcgaagcgc ttgaacatgc tgaggagaat gctgaagaac
     2941 tggaaaagaa agaaaaatcg ctcgagaaag aagtgttgaa tagagcgtat cagctgtctg
     3001 aaaagcgaat gatggcagcg gtttcaatgt catcttcgat aagagagata gttaaagatc
     3061 taggaatgcc agaagcgcag gtaaaccttc gtctgaagag aattgtggac gagagaggag
     3121 gaaatagggc aacgcttcgc ggcatagatg aagcggaatt cttcattgct ccaaaccctg
     3181 gagaaccgga acttcccttg tctagaatcg cttcaggagg agagttgtcg agattcgctc
     3241 ttgcaatcaa ggctgtatta gcttctcatg atgaagtaga gaccctagtg ttcgatgaaa
     3301 tcgattcagg tataggaggc caagttggtg ttgccgttgg cgaatacctg aggcaattga
     3361 gccaatatcg ccaggttcta tgcgttacac atcttgcgac catcgcggtg cgagctgatc
     3421 accaaggcaa agtcgataaa tttgtatcaa aaggcagaac aggtgcctcc atacgctttc
     3481 tttctggaga agaccgggaa accgagattg cacggatgct atccggattt tcagaaagcg
     3541 aagcctccag aattcatgct gctgaacttt tgagaaaagc catttcacat aattgaatag
     3601 tttaaaaaga gaacatgcga gcctattttc atagattata ggtttcagga acttttcaat
     3661 tcctagcctc gacaggcaag ctcatgaaga gcatcatgag gcaaaagctc accgacagtc
     3721 gaacttatat atgaatcttc gctgttgccg aagattaccg gagctgagga gtgcatgaac
     3781 tctgatatca cctgtcggca cgctccacaa gggctgacag ggtaatctgc atccggcgtt
     3841 gctatgacta gtgctttaaa tcgcttcttg ccagaagcga tagccgcggc gatcgcattg
     3901 cgctcggcgc aaattgtcaa tccatagctg cggttttcaa cattgacgcc ggtaatgatg
     3961 ctatcatcgt cgcagagcaa tgccgcacca accctaaact tagaataagg ggcataactc
     4021 tgttcacagg ctttgagagc atgcttgaat agataagtat attccgttgt catgtcagtt
     4081 aagatccttt tccaataata tatccgaaaa tgaacagaat tgaaatgcgc catgcggtat
     4141 tgactcaaag acttcagtca tgcaccataa aatacaatga gaaggcgatt ttatggcctt
     4201 attattggta tagctgcgat tatctttacg ctcttttcaa taagaagcac tttgccggag
     4261 agagagatca catcaagcgt ttttttgaca tttgctgagg ctctggatgt gtcaagaatt
     4321 cctaaagcaa gaagcgatca gatcgtgccc tttattgcag aatccttctt aggttatgcc
     4381 gatccattag aagagctgct ctatgtccat cctttcaagg gaagagcagc aatagataga
     4441 ctcggttgga tcacatatga tcggcttgct accgatttga aattatatga cctgaatggt
     4501 ggtgggcagc aatttcaagc atatgcttat ccatggttta aggcctcatg gagagtattg
     4561 cttcgagccg atcagatggg catagcggta atggacaaga caggattgat taggtgggag
     4621 aaggaatttc cgacgccgat aacggcgatc gatgcatctt ctctgttcct cgccgttgga
     4681 acacttgacg gatcgatcag cgtattcgat ccaaatggat gggctatcct ttcaatgcaa
     4741 aatagtcttc aagaatttca gacaatctat ggtctagccc tatctgcgga cagcaactat
     4801 ctcattatcg taagaggtat ctcacccaag aaaattgaaa cctacaggtt tacagacaat
     4861 tcctttgcaa aggtatccga gtcttcgttg atggtaaata cgactttcca ggcaaccata
     4921 gcaatagccc gagacaattc acacgcaata atgagctttg gtgatatgat aatatattat
     4981 aatataaaga ataaatatta tagaattctt gaagcaggaa tggcaggtgt agagttatca
     5041 gagactcagg ttgattttag gtattttgta cttggatcct taaaagactc gctcgtggcg
     5101 atcctgagaa gcgatctgga aggaaaagca aaatctgatg ttatcattct taatcatggc
     5161 cttccagtac atattgctcc cggagctttt ggcgcaattg gcgatgaaga agtaatcgga
     5221 tttgtatata aagatggcct ggaactatta aaaggatggc ggccatgaag gggaaatatc
     5281 cttttttaat aagttattta tggctcgcta ccttgcttgg tgcattggac ttcaagatac
     5341 ctgaagttat tgatttatca gggtttggaa cagtccttgg cgatcaagct aagcaagggg
     5401 tttcgttaga atatgatgta agagaaagat gctatggcaa gattccaaga agcgatgttc
     5461 agctgatatc gttagataca acgtttgaag caccttcaag cggttctatt ttttattcgc
     5521 aagttgaagc agatttgcca acagtcttct cttttccatt aggtggagcc ctggctgtta
     5581 ttcatcccga tagatttgtg agcataactt cagggctcga tcctattcag gtcataaaat
     5641 caacttcccg atcatcgaaa gaagtccaga atagctttgc aataaagaaa ggagacaagc
     5701 ttactggagg agatggctcg ggcgtatacc catccaaaaa tttcggtgtt agacttttcg
     5761 acgcaaagaa cgaattatgg atgaatacga tttttcttgc tccctggatc agggataata
     5821 catctccaat catgcgcagc gcaaaactga tcatgactgg cacagataag aaggtattct
     5881 ttgatataat ggagacgaat aaaaaagaaa aacttattac ctgtcctcag ggaaattatt
     5941 cattgtttgt cgatgcgcga gatatcatca tcacaagttc gcgttactat tctgctccct
     6001 attgcatcaa ggttacgctt gatggggaaa ccgtaatcga tgcttcattc atagccgcgc
     6061 gcgcaagcgg agaagggata tcatttctgg gcaatccacc cccctcgcga ggatccatag
     6121 gagaaggtta ttcaggagag ggttattcat accatgttgg tcaattcttg attctcagag
     6181 gcaaacacga tcttatgctt caagtgaatg attttgcggg gaatcagact actatgcgaa
     6241 cttccataac cgcatactag aaaagcttgc cccgatttcg gttcgctgat aggattctag
     6301 atatgtcgaa taatagagcg accattgctg ataatgtgac tatcataaaa gaacggattt
     6361 ggcgcgcggc agaaaaggcc gggaggtctt ccgataaaat cgatctcatg gctgtgacca
     6421 aattccagcc tatcgatgtt attcgacagg cctatttggc aggcattaga atctttggag
     6481 aaaacagagt acaggaagca tctgcgaaat ttccttcttt ggaaccagag ctagaaggtg
     6541 ctagcttgca tatgattggc acagttcaga agaataagat caataaagct ttgacaatct
     6601 tcgatgggat tcaggggata gacgatatag aaactctaga ggctatcctt ataagaatca
     6661 aggccccaaa aagaccaata aagctatatc tggagctgca tacgggagag atttcgaaat
     6721 ccggcttccc taatatagat gagctcctgc gagggtgcga ggtctatgca agatatcgtc
     6781 aatcagatcc tcagtggatg acaaaagcct tgctctgcgg tcttatgacc atggcccctt
     6841 ttacgcagga tatttcattg atccgtagat ccttcaagat gctctccgct gcaaaagaaa
     6901 aaataaagag cagctttgat tttccagaat ttagcgagct ttctatgggc atgagcaatg
     6961 attttgagat tgcgatagag gaaggttcga cactcgtgag gataggaact gctatctttg
     7021 gtgagaggaa cgcataaaat gaataggcgc tgccttttat tgctcattgt ttttctatgc
     7081 ggagacctct tggtttattg ttttccagca caagctcaag aagaaaatcc tggctcagaa
     7141 attgtcgcta aagaagaaga gaattctcaa gaagcgccag aggcgcctg
//
LOCUS       DAOOWY010000092         7181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_10435_length_7181_cov_8.203340, whole genome shotgun sequence.
ACCESSION   DAOOWY010000092 DAOOWY010000000
VERSION     DAOOWY010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10435_length_7181_cov_8.203340"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PK105_07015"
     CDS             <1..424
                     /locus_tag="PK105_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU61501.1"
                     /translation="SVYNTLIMKSFFEELPEELKESARIDGASELTVLLRIVLPLSKP
                     ILLTIGLYYGVHFWNDFFNAMLYINSAKLQPLPILLRNILLSSGMNEYVEVSAFGEAP
                     IYAIKAASVFLAAIPMLVAYPFIQKYFVKGTMLGSIKG"
     gene            473..2029
                     /locus_tag="PK105_07020"
     CDS             473..2029
                     /locus_tag="PK105_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU61502.1"
                     /translation="MRKYTRFSLAILLICVVITSAFGQAAKKPVTIELWYGAAITEAG
                     PPPDDWVFYKIAREQLGIDLKLMALPSNENDQDVKILAAAAANQLPDLFMVRRDPWQV
                     LIKQGLVADVDDMYPLMPHRAKNWFDEDSRAHTTINGKSYGLAFPGSLQKNEGLVIRK
                     DWLDRLGLKVPTNLDEFFDVCYAFTYKDPDGNGKNDTYGFGAFVEYFDQEQYLGRRFD
                     PIMGAFGVAGTWNMTKDNFGLNVKKPEFYDALQFVNKMVDAKVIDPDWPSLKKDDFRA
                     AWKQGKFGIMREQFAALAAAANYAPFDKNFPNGEWAVIPPPKGPKGKSSVGIYDTNFR
                     IYAVSAKAAKAGKKEAIARLLDYMATDEGYKLIGWGVEGVNYSIDKDGNITDKGVPND
                     TKFSSPKGQTVTQLRNMVFYNSEMELISRYPYFKTSNGRTMGPLLYLKTMQSMPWTNV
                     TGAGTIRPHPNNADLKRYMNQGVQEFVLGKQALTKENWQAFIVQMDKLGAAEWERAAR
                     QQMEEAGYLQ"
     gene            2068..3387
                     /locus_tag="PK105_07025"
     CDS             2068..3387
                     /locus_tag="PK105_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOU61503.1"
                     /translation="MLTDMTTLYLQTLASAAATSLANCTPASIKWNYETGVLLFSLSE
                     ASTKAFCGLLDGSIQERLSALILPDGSIQGYCLDEFNLDQINAGKLLLREWEASSPEK
                     KADGEEKDLIAGDPDQHTQHRNTSRGCIQCASHRDSLSYRERLEKAIGTLAQQLMGQP
                     RIASGSYWHKKIYPYQVWLDGLYMFGPFQAHYGILFGQTDMVDDVCQQFLFVRDKLRH
                     RQTGLYFHAIDDSKASRWADQETGCSPHVWARAVGWLAMALVDTLDWIPLAHSKRPYM
                     EEMLMDLLDALVREQRASGLWLQVMDAPEAKSNYEETSASAMFAYTFYKSLGRGLVTN
                     ANRAKIWKKTADSAIAGIITKKVRWECASEPSIQLLQIEPMAELSTFKSTMPRLHLDG
                     ICSVAGLGGSPYRDGSIAYYVGEPIVLDDFKGLGPFMLALTEALHAN"
     gene            complement(3389..4387)
                     /locus_tag="PK105_07030"
     CDS             complement(3389..4387)
                     /locus_tag="PK105_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61504.1"
                     /translation="MNDSAILISIGGVLASAAPIIFAVIGETISERAGVLNLSMNGLI
                     LICAMGSFAVALATDSSILGFLTGAAIGALVSLIVAFASLTLLQSQVAVGFVLAMLCK
                     DLSYFLGSPIMGKVAPRISQVPIPLLERIPILGPIFFRQDMMTYLSFVLIVLAWFFIF
                     KTKPGLILRGIGERPAAAYIRGANVTRLQYLYAVAGGAIAGLAGPIYSLSIKAGWKGT
                     ISGLDGIGWIVLSITIFGGWKPFRAAFGAYLFSFLQWLGLVLQPSLPSIPSQVLQVAP
                     FPLMILTLLFVNIGNAEWVERSLARLPENVRRDIAKVLRALNATPPAALGEPFRKE"
     gene            complement(4384..5445)
                     /locus_tag="PK105_07035"
     CDS             complement(4384..5445)
                     /locus_tag="PK105_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61505.1"
                     /translation="MPSLREKKASGVLFQIAAVLFALLITSIVLLLANVEPFSAYKAI
                     ILGAVGSWDLMSNVLVSWVPLLLATSGLLLTFTVGLWNIGIEGQITLGAIFTTWAMRL
                     LQNSEWNPSLILVIAIFAGMLGGMLWAMLAGALKTFGGVNEIFGGLGLNFVATALNIW
                     LIFGPWKRPGVASMSGTLPFDRSLWMPIATGSSRLTPTTIVMAIVGILIVYVLLENTY
                     FGLKLKAVGKNPRSAYLLGVPTWQHMMASFAICGVFAGLAGAVQVTAVYHRLIPSISS
                     GYGYLGLMVAMLVGYKASLAAPVALFFAALNVGSIQLPIILRLDSSLAGVLQGTLVLC
                     VLLGQGFRAKFLKKKRTMQ"
     gene            complement(5429..6937)
                     /locus_tag="PK105_07040"
     CDS             complement(5429..6937)
                     /locus_tag="PK105_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU61506.1"
                     /translation="MQVEFRGITKCFGPLRANDDISFIIPSSTIQGLLGENGAGKSTL
                     MKILSGYYSSDAGEIYLNGKKVDISCPGDAIDLGIGMLHQDPLDFPPLKVIDNFILGK
                     KGGILPNRGSAIREFEELADRFDFSIDPEAYVDSLTVGERQQLEILRLLWLGARVLIL
                     DEPTTGISLPQKEKLFATLKKLAEQGMTILFVSHKLEEVEQLCDRAIVLRQGKLIGAM
                     DPPFNTKALVEMMFGKEIELEYRAKLSIGQKVLHAKSICLEGTRINIKGVDLEVRAGE
                     VIGLAGMEGSGQTMFLSACAGLTQPVGGSLCLGEEDKDMTGRSYLEFLKHGVAYLPAA
                     RLEQGLICGLTLMEHFVLSEKMPGFFIDRKRSEELTKERIALYNIKGAPDSPIESLSG
                     GNQQRALLALLRDHLELILVEHPTRGLDYESTIYIWGKLKQRCANGTAIIFISSDLEE
                     ILTYSDRVLVFFAGKVTPPLCAEKLNVERLGRLIGGKDWENLEKEAVYAVFA"
BASE COUNT         1988 a         1725 c         1728 g         1740 t
ORIGIN      
        1 aagtgtctat aacacgctca taatgaaaag cttttttgag gaactgccag aagagttgaa
       61 ggagtccgca cgcatagatg gggcttcgga gttgacggtg cttctaagaa tcgttttgcc
      121 gctctcaaaa ccaattctac tcacaattgg cttgtactat ggtgttcact tctggaacga
      181 ttttttcaac gccatgctct atatcaacag tgcaaagctc cagccgctgc cgatcctatt
      241 gcggaatatc ttgctttcct cgggtatgaa tgaatatgtc gaggtcagcg ccttcggaga
      301 agctccgata tatgccataa aggcagccag tgtgtttttg gctgcaattc ccatgttggt
      361 cgcatatcca tttattcaaa agtactttgt aaagggtacc atgctcggaa gcatcaaagg
      421 ataagagcaa aaaataattg agaagcggca ttttgccgca aaggaggttg gtatgagaaa
      481 atatacaagg ttcagcttgg ccattcttct catctgtgtt gtcattacgt ctgcttttgg
      541 tcaagccgca aagaagccgg tgacgatcga gctgtggtat ggggcagcca ttaccgaggc
      601 aggcccgcca ccggatgatt gggtgttcta caagatcgct cgcgaacagt tgggaatcga
      661 tctaaaactc atggctcttc catcgaacga aaacgatcag gatgtaaaga tccttgccgc
      721 agcagcggcc aatcaattgc ccgatctttt catggttcgc cgtgatccat ggcaggttct
      781 tatcaagcaa ggacttgttg ctgatgtcga tgatatgtat ccccttatgc cacaccgagc
      841 caaaaattgg ttcgacgagg atagcagggc gcatacgaca ataaatggca agagctatgg
      901 tctggctttt cctggttcgc ttcagaaaaa cgaagggctt gtgattcgca aggattggct
      961 ggacaggctt gggctcaagg taccgactaa tctggatgag ttcttcgatg tttgctatgc
     1021 ttttacctac aaggatccgg atgggaatgg aaagaacgat acctatggct ttggagcttt
     1081 tgtcgaatac ttcgatcagg aacaatatct tgggcgtcgc tttgatccca taatgggagc
     1141 ctttggagtt gccggaacct ggaacatgac aaaggataat tttggactaa atgtcaaaaa
     1201 gcccgagttt tacgatgctc tgcaattcgt caataagatg gtcgatgcaa aggttatcga
     1261 tccagactgg ccgagtttga aaaaggatga cttccgcgca gcgtggaaac aaggcaagtt
     1321 tggaattatg cgcgagcaat ttgccgcgct tgcagcggca gccaactacg ctcctttcga
     1381 taagaatttc ccgaatggcg agtgggctgt gattcctcct cccaagggtc cgaaaggaaa
     1441 gagctctgta ggcatctatg atactaattt ccgcatctat gcggtctctg caaaggccgc
     1501 taaggcaggt aaaaaggaag ctatcgctcg ccttctcgac tacatggcga ccgatgaagg
     1561 ctacaagctc ataggttggg gagtggaagg agtcaactat tcgatagata aagatggcaa
     1621 tattaccgat aaaggcgtac cgaacgacac gaaattctcc tcgccaaaag gccagactgt
     1681 cactcagctt cgcaacatgg ttttctataa cagcgagatg gagcttatct cgcgttatcc
     1741 atacttcaag accagcaatg gtcgcaccat gggcccattg ctttatctaa agacgatgca
     1801 gagcatgccg tggaccaatg tgacaggcgc tggaacaata aggccacacc ccaacaatgc
     1861 cgacctaaag cgctatatga atcagggggt tcaggagttt gttcttggta aacaagcgct
     1921 gaccaaagag aactggcagg cctttattgt ccaaatggat aaacttggag cagctgagtg
     1981 ggaaagagca gctcgccaac agatggaaga agccggctat ctccaatgaa gagttagctc
     2041 tatagatcta cttagagggt agccgcgatg cttaccgaca tgacaacctt gtatctgcag
     2101 actctagcat cggcagctgc gacttccctt gccaactgca cacccgcctc aatcaaatgg
     2161 aattatgaga cgggtgtgct tctattttcg ctcagtgaag cctccacaaa agcgttctgt
     2221 ggtcttttag acggctctat tcaggaaagg ctctccgcgc tgattcttcc ggatggctcg
     2281 attcaaggct attgtctcga tgaattcaat ttggatcaga tcaatgcggg aaagctgctt
     2341 ctccgtgagt gggaagccag cagcccggag aaaaaggcag atggcgaaga gaaagatctc
     2401 atagctgggg atcctgatca gcacacgcag cacaggaata ccagtcgtgg gtgcattcaa
     2461 tgcgcaagcc atagagatag cctgtcttat cgcgaacgac tggaaaaagc aatcggaact
     2521 ctcgcgcaac agctaatggg gcagcctcgt atcgcttctg gctcatattg gcataaaaag
     2581 atatatccgt atcaagtatg gctcgatggt ctttatatgt ttggaccttt tcaagcgcac
     2641 tatgggatcc tctttggaca aacggatatg gtagatgatg tatgccagca gttcttgttt
     2701 gttcgggata aattgaggca tagacaaact ggactatatt tccatgccat agatgatagt
     2761 aaggcatcta gatgggcaga ccaggaaact ggctgttcgc ctcatgtatg ggctagagcc
     2821 gttggctggc ttgcaatggc gctcgtggat acgctcgatt ggattcctct tgcgcattct
     2881 aagcgacctt acatggaaga gatgcttatg gatcttctcg atgctcttgt gcgagaacag
     2941 cgtgcttctg gcctatggtt gcaggttatg gatgctccag aggctaagag caactatgag
     3001 gagacttcgg cttcggccat gttcgcttat acattctaca agagccttgg aagaggttta
     3061 gtaacgaatg caaatcgagc caaaatatgg aagaaaaccg cagactcagc gatagccggg
     3121 ataataacaa aaaaagtcag atgggaatgc gcttctgaac cttcgataca gcttctacaa
     3181 atagaaccga tggcagaact atcgacattt aagtctacaa tgccgcgcct gcatctcgat
     3241 ggaatatgca gcgttgctgg tcttggtgga tctccatatc gcgatggctc gattgcctac
     3301 tatgtcggag aacctattgt attggacgat tttaaaggcc ttggtccttt tatgcttgca
     3361 ttaaccgaag ctctccacgc caattaaatc attccttcct gaatggctcg ccaagagctg
     3421 caggtggggt ggcattcagg gctctcaaga ccttggctat atctctacgg acattctcgg
     3481 gcaaacgcgc aagcgatcgc tcgacccatt ccgcgttgcc tatattcaca aatagcagtg
     3541 tcaggatcat aagagggaag ggtgccacct gtagcacttg ggatgggata gatggcagac
     3601 tgggctgcaa cacaagacct aaccattgaa ggaaagaaaa aagatatgct ccaaaagctg
     3661 ccctgaatgg cttccagccg ccaaagatcg taatggagag tacaatccag ccgataccat
     3721 cgagccccga aatagtgccc ttccaccctg cctttatgga aagcgaataa atagggcctg
     3781 ccagtccggc aattgctcct cctgccaccg catacagata ttgcaagcgg gtcacgtttg
     3841 cgccgcgaat ataagctgcg gcgggtcgtt ctcctatgcc tcgtagaatg agaccgggct
     3901 tggtcttaaa gataaagaac cacgcaagca caatcagcac aaaagaaaga tatgtcatca
     3961 tatcctggcg aaagaatatg gggccaagaa ttggaattcg ttcgagcaaa ggaataggta
     4021 cctgtgagat acgtggtgct acctttccca tgattgggct gccaagaaaa taagacaaat
     4081 ccttgcatag cattgccaac acaaagccga cagctacctg agactgcaaa agagtcaggc
     4141 ttgcaaaagc cactatgagc gagacaagag ctcctatcgc tgctcctgtc aaaaaaccca
     4201 agatcgagct atcagtcgcc agagccaccg caaaactacc cattgcgcag attagaatca
     4261 acccattcat ggaaagattc agcactcctg cccgttcgga gattgtctca ccaatgacag
     4321 caaagatgat aggcgcagcc gaagcgagca ccccaccgat acttattaga atcgcagaat
     4381 cgttcattgc attgtccttt ttttctttag aaacttagcg cggaaaccct gtccaagaag
     4441 gacgcaaaga acaagcgttc cctgaagcac tccggccaat gaagaatcca acctgaggat
     4501 gataggcaac tgaatagagc cgacattcag agcagcgaag aacaaggcaa ccggagccgc
     4561 gagacttgcc ttatacccta ctaacattgc taccataagc cccaaataac catagcccga
     4621 tgaaatcgaa gggataaggc ggtggtaaac ggcggtaacc tgcactgctc cggcaaggcc
     4681 tgcaaaaaca ccgcagatgg cgaaagacgc catcatgtgc tgccaggtcg gaacacccag
     4741 caggtatgcg ctccttggat tcttgcctac tgcctttagc tttagaccaa aataggtgtt
     4801 ctcaagcaac acatagacaa tcaagatacc gactatagcc atgacaatag ttgtaggcgt
     4861 tagcctagat gaaccagtag cgattggcat ccatagggag cggtcgaagg gcaaagtacc
     4921 tgacatcgaa gcaacaccag gacgtttcca tggtccaaag attagccaga tattgagagc
     4981 tgtcgctaca aagttgagtc caaggcctcc gaaaatctca tttactcctc cgaaagtctt
     5041 gagcgcaccc gcgagcatag cccatagcat tcctcctaac atcccagcaa agatcgcaat
     5101 gaccagaatg agtgaaggat tccattcgga gttctgcaaa agccgcatag cccaagtcgt
     5161 aaatatcgcg ccaagcgtaa tctggccctc tattccaatg ttccacaagc ccaccgtaaa
     5221 ggtcaaaagt agccctgagg ttgccaggag caaaggcacc caggaaacga gaacattgct
     5281 catcaaatcc caggaaccta ctgctcctaa gattatggct ttataggcag aaaagggttc
     5341 gacattagcc agaagaagca cgatagatgt aatcaacagt gcgaataaaa cagctgcaat
     5401 ctgaaataga acgccactgg cctttttttc acgcaaagac ggcataaaca gcttcctttt
     5461 ccaggttttc ccaatcctta ccacctatga gtcggcccaa acgctccaca ttgagctttt
     5521 ctgcacaaag tggcggcgta accttgcctg caaagaacac gaggactcta tcgctatatg
     5581 ttaggatttc ttcgagatcc gaagagatga agattattgc ggttccgtta gcacatctct
     5641 gtttgagctt gccccaaata tagattgtcg attcgtagtc aagacctcgt gttggatgct
     5701 cgaccaatat gagctcgaga tggtctcgca gaagtgcaag aagagctctc tgctgattac
     5761 ccccggaaag cgattcaatt gggctgtcgg gtgctccctt tatgttgtag agagcgatgc
     5821 gctcttttgt aagctcttcc gatcttttgc gatcgatgaa gaaaccgggc attttttccg
     5881 aaagcacaaa atgctccatc agagtaagcc cacagattag tccttgctcc aaccttgccg
     5941 cgggtaggta agcaacgcca tgcttgagaa actccagata agagcggcct gtcatatctt
     6001 tgtcttcttc gccaaggcag agtgagccgc cgacgggttg agtaagcccg gcacaggcac
     6061 taagaaacat agtctggcca gaaccttcca tacctgcaag accgataacc tcgccagccc
     6121 taacttccag atcgactccc tttatattta ttctagttcc ttccaggcag atactttttg
     6181 cgtgcagcac tttttgtcct attgatagct tagccctgta ttcgagttct atctctttac
     6241 cgaacatcat ttcgacaagc gccttggtat tgaaaggcgg atccatagct ccaatcagct
     6301 ttccctgtcg aaggacgata gccctgtcgc aaagctgctc cacttcttcg agcttatgag
     6361 atacaaaaag aatggtcatg ccttgttctg ctagtttttt cagagtggca aagagttttt
     6421 ctttttgtgg caggcttatg ccggttgtgg gctcatcgag aataagcacc cgagcgccta
     6481 accacaaaag acgcaatatc tctaactgct ggcgctcgcc cacggtaaga gaatccacat
     6541 aggcttctgg atctatcgaa aaatcgaagc ggtctgcaag ctcttcgaac tctctaattg
     6601 cagaacctcg gttgggcaga atgcctcctt ttttaccgag aatgaagttg tcgattacct
     6661 tgagaggcgg gaaatccaat ggatcttgat ggagcatacc gattcccaaa tctatggcat
     6721 caccgggaca agagatatcg acttttttgc catttaagta gatttcgccg gcatcagagc
     6781 tgtaatagcc cgagagtatc ttcatgagag tgcttttacc tgccccgttc tctcccagaa
     6841 ggccttgaat ggtagaggag ggaattatga atgaaatatc gtcgtttgct cgcaatgggc
     6901 caaagcactt tgttattcca cgaaactcga cctgcattga atccttcttc tagtctattt
     6961 gatctcgctc atttaagatt gaagcaatga gcgtcaattc aagtctgaag catgtcaaaa
     7021 atagagcgcg gaggcaagcc gaccaaggac cgtcagctta tgcctccgac atactagaga
     7081 aaaagctctc tactatatga aataaagaag acaattactt cgatactgat tgtccttcca
     7141 tgccctgcaa gagctgtggc aggtaccaga tctgctgatc t
//
LOCUS       DAOOWY010000093         7143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_10517_length_7143_cov_8.096642, whole genome shotgun sequence.
ACCESSION   DAOOWY010000093 DAOOWY010000000
VERSION     DAOOWY010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10517_length_7143_cov_8.096642"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PK105_07045"
     CDS             complement(<1..1101)
                     /locus_tag="PK105_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61507.1"
                     /translation="MDEYFDAEPVTKGYDAQIARRILLYLKPYGVYAIIAILALAIAT
                     TGELISPTIIRRAIDDSVVRDWYGLDPSALSMLPGNDSSHEVEIGGRIYVRSSNLAGL
                     TMAERDHLREQGLLDNEASYVFPLDPMRDKKLSVMDGIPKAELSDKWGVIPSSVLRTL
                     KSEDAAILRAADDKSLLRFGFILLVVLLIVLGATFAMTWFTNQISTLIMKDMRLELFH
                     HVMEQSLSYLSRQPVGRLVTRLTSDIEVISQFFSDVLSAFIKDASIMIGSLIVLFLLS
                     WKLALIVLTTMPFVFIAAAIARIKARDAFRNQRYWTSKVNSYLSEHISGIDIVKLFVQ
                     EDKVSENFSKNSKQLLKANIAEMYVYATFRPFV"
     gene            complement(1094..2935)
                     /locus_tag="PK105_07050"
     CDS             complement(1094..2935)
                     /locus_tag="PK105_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61508.1"
                     /translation="MLKEYATLLPHLRSYRYRYVAGILCLIVVDASQVLIPRYIQIAI
                     DRISSGAFALVDIIDPLVHMLLLALLISIGRFFWRYFINIASRRIEAEMRDRLFSHIL
                     SMSGNFFRENTTGDLMARATNDISTIRQATGMGFVSLIDGIFMTAMIIAAMFLNNSAV
                     AAWIILPLPLITALILLFGRIVGRLFKKIQDIYGYLSNIAQESLSGIRIVKSFVKEDY
                     FYNRFSMANTDYKNAIMDLVKTSGFFFPFITFLSGLSTILLIIFGGKASIRNKMTPGS
                     IIAMLSYLEMLVWPLMSAGFTVNIVQRGAASLKRINQILRTAPEIKESSNCIRKTPRG
                     DIDIRRLYYIYPGSQKPALKDISVHIPEGSMLGILGKVGSGKSTLLKILPRMLEPGEG
                     HVFIGNIDIWSYGLRELRSAFGMVPQESFLFSDTIEHNILFSAHNISPERFKAITKIS
                     GIDHDYQLFPDGWETVVGERGITLSGGQKQRIALARALAADPNILLLDDALSAVDAQT
                     EEHILSSLIDERKAKTTIIVSHRISTLRNADYIIVLDSGEIIQQGSHEELMAESEGFY
                     ARIAALQQLEQQVSIDSIKNNISQRETISREKPLGQDTSVSEEKIYG"
     gene            3012..3521
                     /gene="rnhA"
                     /locus_tag="PK105_07055"
     CDS             3012..3521
                     /gene="rnhA"
                     /locus_tag="PK105_07055"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOU61509.1"
                     /translation="MNQQEFLVYTDGGCKGNPGPGGWAYVMHYGDRYREAWGGELYTT
                     NNRMELIAVIKALSFLKSRIENASSSVSEESLEAPSWTRAKINIFTDSMYVKNGITEW
                     LIEWKKRGWKTSAKKPVKNLELWQELDSLCCELKPHFEWIEGHSGNIDNERCDRLVHL
                     AIEDVLCQK"
     gene            complement(3678..5135)
                     /locus_tag="PK105_07060"
     CDS             complement(3678..5135)
                     /locus_tag="PK105_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61510.1"
                     /translation="MRKSIIICLVVLFGLVTSCASVPSIPEWVKKTPSPDAKYTYFVG
                     SSSAKDSATALNDATAALIAGIMQYMGVSISVASSAEARASLSEYEAQITQTVKTEAK
                     GRLSGFEVVEKFIEQDKKSGLYTVHVLARYETKELNKEKARMESLLKEKADAVAIPEQ
                     KGDAAANEGLLIDAIRAYAEAMSAAASSEVENAQIKLARNAKKASELAATLRLAILSP
                     SNIEIQLGGAKVPFITVKLIANRGGREEGVPGAPIAITYPRRLTSGRIGTATQQVFTN
                     QDGIVSFDVPPIELAGKYQVTAQLDFGSIADILGGLPKWALPYIDALESELSGIRASM
                     SYKVISAAKNVPTAIGVYLKSEAQKGSLDPEPFSKSLEEALVNEGFSIFTTKISAESG
                     QPDIDRLRAIAPAEAMRFVLACVELISIGKEDGFFIASVAGSLSAIDLIEGNTLYSGS
                     KGAQGMGLSEAEAISSALRTLGGQVFAKELLAALR"
     gene            complement(5240..5893)
                     /locus_tag="PK105_07065"
     CDS             complement(5240..5893)
                     /locus_tag="PK105_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61511.1"
                     /translation="MKARKAVLVLLVAAVIISCGTKPPLRKGEVLIEDKGTAYGIKTP
                     KWVELAIIGGYRDIEKLPDYKDKVVFIAQFEAQNLQSAQLLAERMQADTEIARYLSTR
                     VKDAFKGANVADADSKNFGAYGERFVMSVGEATFSGFRMEADWWVKVQTYTPENKPDK
                     QIYRVIQLWAIDKNMLQKQFDMLFSQMAGGEPPTPEQKRAMDLVQNTVTKDFFGESD"
     gene            complement(5932..7017)
                     /locus_tag="PK105_07070"
     CDS             complement(5932..7017)
                     /locus_tag="PK105_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61512.1"
                     /translation="MKRAWSFYRYLMGVLLLIGLLIGCATTPQPEAPSLESQRAKEIV
                     QGTSTPEMPGTTAPGDVQSSAEALVESQVMPGETPAPSEPSSVPQASASYYTQPMTAD
                     ESRFFQNYINRLSYLVYYNENSGLDAQLAKAAVSQANRYLIEKEGLSVIDFDQIQKNK
                     KDQISAYQSETGGSIDIITYIAQKLNADIYLEIDAKTSFGGGPGAWTGSAQGSIKIFD
                     ASTAALLGSISFLSPQTFSPVSADAAMMNAISGIIWQSMPKVTEQAKHLMSTMTASRG
                     VRYELILQNTPDAIAISQFERNLAKRVREVERLSYSPGETRFAIYAFMPASKIQDAIY
                     DAASMSGFPNCYLLYMRGRSYTFNTGI"
BASE COUNT         1984 a         1637 c         1506 g         2016 t
ORIGIN      
        1 gacaaaaggt ctgaaggtgg catagacata catctcggca atattggcct ttaacagctg
       61 cttgctgttt ttgctaaaat tttcagaaac cttatcttct tggacaaaga gcttgacgat
      121 atctattcct gatatgtgtt ccgagagata agaatttacc ttgctcgtcc aatagcgttg
      181 atttctgaaa gcatctcgcg ccttaatgcg tgcaattgca gcggcgataa agacaaatgg
      241 cattgtagtc aatacgatta atgctagctt ccaactcaac aagaagagca ctattagaga
      301 ccctatcatg atagaagcat ctttgatgaa agcgctcaac acatcgctaa agaactggct
      361 gatgacttct atatcgctcg ttaatctcgt aaccaagcgc cccacgggtt gcctcgaaag
      421 ataagaaagc gactgctcca tcacatggtg gaatagctcg aggcgcatat ccttcatgat
      481 caatgttgaa atctgattcg tgaaccatgt catagcgaag gttgcgccaa gtacgatcaa
      541 tagcacaacc aacaaaatga atccaaagcg taagagtgat ttatcgtctg ctgctcgaag
      601 aatcgcggca tcttcagatt ttagggtcct caaaaccgaa gatggaatca ccccccattt
      661 gtccgaaagc tctgctttgg ggattccatc cataacagac agctttttat ctcgcattgg
      721 atcaagagga aatacatagc tcgcttcatt atcaaggagg ccttgttcgc ggagatgatc
      781 tctttcggcc attgtaagcc ctgcgagatt agaggagcgc acatagatgc gtccgccaat
      841 ctcaacttcg tgagaactat catttccagg cagcatggaa agcgcagagg gatctaatcc
      901 ataccaatct cgaacaactg aatcatcgat ggcacgtctt ataattgtag gagatataag
      961 ttctccggta gtagctattg ccagggctag aatagctata attgcatata ctccatatgg
     1021 tttgagataa agaagaattc tgcgcgcaat ctgtgcatca tagccctttg tgacaggctc
     1081 tgcatcaaag tattcatcca tagatttttt cctctgacac agaagtatct tgccctaatg
     1141 gtttttctct tgatattgtt tctctttgag aaatattatt cttaatagaa tcgatagaga
     1201 cctgttgctc aagttgctgc aaagccgcaa ttcttgcata gaaaccttcg ctttccgcca
     1261 ttagctcttc atgtgaaccc tgctggatta tctctccgct atcgagcact atgatataat
     1321 ccgcattacg cagggtcgaa attcggtgcg agacgattat ggtagtcttg gcttttctct
     1381 catctatgag agagctaaga atatgctctt cggtttgcgc atccactgcg gataaggcat
     1441 catctagtaa gagaatattt ggatcggccg caagggctct ggccagagca atcctttgct
     1501 tttggcctcc agagagcgtt attcctcttt ctcccacaac tgtctcccac ccatcgggga
     1561 aaagttgata gtcatgatcg attccagaaa tcttagttat ggccttaaag cgttcggggc
     1621 tgatattatg cgcagaaaaa aggatgttat gctcgatggt atctgagaat aggaagctct
     1681 cctggggcac cattccgaaa gcagatcgaa gctcacgtaa tccatatgac catatatcta
     1741 tattgccaat aaatacatgg ccttctccgg gttctagcat tctaggaagg atttttaata
     1801 gtgtgctctt gccagatccc acttttccga gaattcctaa catcgagcct tcaggaatgt
     1861 gaacagaaat gtcctttaag gcgggcttct gcgagcctgg ataaatataa taaagtctcc
     1921 taatatcgat atctccgcgg ggcgtctttc ttatgcaatt ggatgattcc ttgatttcag
     1981 gcgccgttct gagaatttgg tttattctct tcaagcttgc ggcaccccgt tggacgatat
     2041 tcacggtaaa accagcgctc atcagcggcc ataccagcat ttcgagatac gaaagcatag
     2101 cgatgatcga acctggcgtc attttgttcc gaattgaagc tttaccgcca aagattataa
     2161 ggagtatcgt gctcaatccg gagagaaaag tgatgaaagg gaaaaagaag cctgaagtct
     2221 ttacgagatc catgatggca ttcttataat cggtattggc cattgagaat ctgttataaa
     2281 aataatcctc ttttacgaag gattttacaa tgcggatgcc agaaagggat tcctgggcga
     2341 tattcgacaa gtaaccatag atatcctgaa tcttcttgaa tagtctcccg actattcggc
     2401 caaagagaag gatcaaggca gtaatcaaag gcaaaggaag gattatccag gcagcaaccg
     2461 cagaattgtt caagaacatt gcagctataa tcatggctgt catgaaaatg ccatcgataa
     2521 gagatacaaa tcccataccg gttgcttggc gaattgtgct tatatcgttg gttgccctgg
     2581 ccattagatc gcctgtagta ttctccctaa agaagttgcc cgacatcgac aagatatgtg
     2641 agaatagcct gtctctcatc tctgcctcga ttcgtctgct tgcaatattg ataaagtacc
     2701 gccagaagaa tcgtcctata gatatcaaga gcgcaagcaa tagcatatga accaaaggat
     2761 caatgatgtc aacaagagca aaagctccgc tagaaatgcg atctatagct atttgaatat
     2821 atcggggaat gagaacctgg cttgcatcta ctacaataag gcataagatg ccggcaacat
     2881 atcgatatct atagcttctc agatgaggta acagcgttgc gtattcctta agcatatatc
     2941 tttattctaa cgatggcttg gaaactcagc aaggagttgc ttgaatcgga gtcttctgcc
     3001 atattgcatc catgaatcag caagaattcc ttgtatatac tgatggtgga tgcaaaggta
     3061 atcctgggcc tggtggatgg gcttatgtaa tgcactatgg cgaccgatat cgagaagcat
     3121 ggggtggaga gctttatacc acaaataata ggatggaact cattgctgtt atcaaagcgc
     3181 tgtcctttct aaaatcaaga atagaaaatg ctagttcttc ggtttccgaa gaatcactag
     3241 aagccccctc ttggacaaga gctaaaatca atatattcac cgattcgatg tatgtaaaaa
     3301 atggtattac tgaatggctg atagaatgga aaaaacgcgg ctggaaaacc tctgcaaaaa
     3361 agcctgtaaa gaatctcgag ctatggcaag aattggatag cctatgttgc gagctaaagc
     3421 ctcatttcga gtggatagaa ggacattcag gcaacatcga caacgaacgt tgtgacagat
     3481 tggtccatct cgccatagaa gatgtacttt gccagaaata gagactactg ctacgaacct
     3541 tgtgtataaa aagttgaagt aagaaacagg aaatagaacg aataagggga gaacaaggag
     3601 ctaactatgc agactttccc tttctgccct aatcccaaat gcctctataa tatatacaat
     3661 catttttaag agaattctca acgcaaagca gccaatagtt ccttggcaaa aacttgacca
     3721 ccaagtgtcc tgagtgccga ggaaattgcc tctgcctcag aaagccccat accctgagcg
     3781 cccttgctcc ccgaatagag agtatttcct tctatcaaat caattgcact cagagaacct
     3841 gctaccgaag cgataaagaa accatcctct ttcccaatcg aaatcagctc aacgcaagcc
     3901 aaaacaaagc gcatggcttc agcaggagca atagctctta gccgatcaat atcaggctgt
     3961 ccggactctg cagagatctt tgtcgtaaaa atagagaagc cttcatttac gagtgcttct
     4021 tcaaggctct ttgaaaacgg ttcaggatct aggctgccct tttgggcttc gctcttaaga
     4081 tagactccaa tcgctgtagg aacattctta gccgctgaga ttaccttata gctcatagat
     4141 gcccttatgc ccgacaattc gctttctaat gcatctatgt agggcaatgc ccattttggt
     4201 aatcctccca gaatatctgc aatgctacca aaatccaact gagcagtcac ttgatacttg
     4261 ccagctaatt ctattggagg gacatcgaat gaaacgatgc cgtcttggtt ggtgaatact
     4321 tgttgtgtag cggtacctat tcggcctgaa gtcagtcttc gcggataagt tatagcaatc
     4381 ggagcacccg ggactccttc ttctcttcct ccgcgattag ctatgagttt caccgtaata
     4441 aatggaacct ttgcaccgcc cagctgaatt tcaatattcg atggagataa tattgccaat
     4501 ctgagagtgg cggctaattc ggaagctttc tttgcatttc gagcgagctt tatctgtgca
     4561 ttctctacct cagagcttgc agccgccgac atggcttctg cataagcgcg aatagcatct
     4621 atcaggagcc cctcattggc tgcggcatca cccttctgtt caggtatagc aaccgcatcg
     4681 gctttttcct tgagtaatga ttccatccga gctttctctt tatttagctc ctttgtttca
     4741 tatcgagcga gtacatgcac tgtatataac ccactttttt tatcctgttc tataaatttt
     4801 tcgaccacct caaaacccga aagccgacct ttagcctctg ttttgactgt ctgtgttatt
     4861 tgggcttcat attcactgag agaagctctt gcttcggcgc tggaagccac gcttatcgat
     4921 actcccatgt attgcataat acccgcaatt aaagccgcgg ttgcatcatt tagggcagtc
     4981 gcgctatcct ttgccgaaga agaacctaca aaataagtat attttgcatc agggcttggc
     5041 gttttcttta cccattctgg aatgcttggc acactagcgc aactagtcac aagcccgaaa
     5101 agaacaacca gacatataat gatgctcttt ctcatttatc attcctctct gggtaagaaa
     5161 gcctacgata tatttaggag aacctcggca tcatatctta taccatgcac cgaggcctcc
     5221 ccatgaaagg attttctcat caatcagatt cgccaaagaa atctttggtt actgtgttct
     5281 gaactagatc cattgcgcgc ttctgctcag gcgttggagg ctctccccct gccatctgag
     5341 agaatagcat atcaaactgt ttttgaagca tattcttatc gatcgcccat agctgaatta
     5401 cccgataaat ttgcttgtca ggcttatttt ccggagtata ggtctgaacc ttaacccacc
     5461 aatcagcctc catcctaaag ccagagaatg tagcttctcc cacactcatg acaaaacgct
     5521 cgccatatgc gccaaaattc ttggaatccg catctgctac gtttgcacct ttgaatgcat
     5581 ctttcactcg cgtcgataaa tagcgagcaa tctcggtatc agcctgcatt cgctccgcga
     5641 gcaattgtgc actctggaga ttctgagctt caaattgagc tataaaaacc actttatctt
     5701 tataatcggg tagtttttca atatctctgt agccacctat aattgcaagc tctacccatt
     5761 ttggtgtttt tatgccatat gcagtgcctt tatcctcaat cagcacttca ccctttctaa
     5821 gggggggctt agtaccgcat gagatgatca ctgcagcgac tagcaaaacc aataccgcct
     5881 ttcttgcttt catcttttcc tcctaataat gaacaacatt atgaaaatgc actaaattcc
     5941 cgtattaaat gtgtatgatc tgcctctcat gtatagaaga tagcaattag ggaaaccgga
     6001 catcgaagcc gcatcatata tagcatcttg aatcttcgaa gctggcataa aagcatatat
     6061 agcaaatctg gtttcaccgg gtgaatagga gagtctttca acctcacgta ccctttttgc
     6121 caaattcctt tcgaattgag atattgctat cgcatcagga gtgttctgta gtatgagttc
     6181 gtatcgaact cctcgagatg cggtcattgt gctcataaga tgctttgctt gctccgtgac
     6241 ctttggcatc gattgccaga taatccctga tatagcattc atcattgcag catctgcgga
     6301 gacagggctg aatgtctgcg ggctcaaaaa ggatatcgat ccaagaagcg ccgcagtaga
     6361 tgcatcgaaa atctttatac tgccttgtgc tgatccggtc caggctccag gccctcctcc
     6421 aaagcttgtc ttagcatcta tttcgaggta gatatctgca ttgagcttct gtgcaatata
     6481 agtgatgata tcgatagagc cacctgtctc agactgataa gcgcttatct gatctttctt
     6541 attcttctgg atctgatcga aatcgatgac agaaagccct tctttttcga ttaggtatcg
     6601 atttgcctgt gatacagcag cttttgcaag ctgtgcatcc aagccactgt tctcgttgta
     6661 atacactaag tagcttagtc tgttaatata attctgaaaa aatctgcttt catcggcagt
     6721 catcggttga gtataataac ttgcgcttgc ctgtgggaca gaagaaggct ccgatggagc
     6781 cggagtctca ccaggcataa cttggctttc tacaagcgct tctgcggagg attgaacatc
     6841 tccgggagcc gttgtgccgg gcatttctgg tgtcgaagtg ccctgaacaa tctcttttgc
     6901 tctctggctt tccagactag gtgcttctgg ctgaggagtt gttgcgcaac cgataagtag
     6961 accgataagc agcagaacac ccataagata tctataaaag gaccatgctc ttttcatgtt
     7021 cttattatga gatagcattt gtacttttgc aagatgaaaa tgctaaatgg gacaaaagta
     7081 cgaaaggttt ttctctgagc gatgcgcgat ttgaacgcgc gaccttcggc tccggaggcc
     7141 gac
//
LOCUS       DAOOWY010000094         7101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_10600_length_7101_cov_10.730911, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000094 DAOOWY010000000
VERSION     DAOOWY010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10600_length_7101_cov_10.730911"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK105_07075"
     CDS             complement(<1..414)
                     /locus_tag="PK105_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU61513.1"
                     /translation="MSMLLLKNGEVYAPCPLGSRDILIGSDRIISIGSNIDLSDSIPD
                     VETIDASGCIVVPGLIDGHQHFIGGGGEGGFETRTPELQISMNFLNGVTTAIGLLGTD
                     SLTRSLEDLYAKTKAFNTEGMTAFMLTGAYWYPSPS"
     gene            complement(444..1313)
                     /locus_tag="PK105_07080"
     CDS             complement(444..1313)
                     /locus_tag="PK105_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOU61514.1"
                     /translation="MKRMVFDKIRKMLPEMANSYQKIGSYMLDHEQSVAFSSIHSISK
                     SIGVSSATLVRFAKSLGFQGYQEFKHEIQDEIRHRLNPYDKIALSELDILPGEKRLQK
                     LFQNEYNNTRSTFANIKIADLEIMGEGIKKARRVYLSAFGATAYVAHIFKYTLMSSLE
                     KEVYVVSGSVSDYAPVLKSFSSEDVMFLMTFPPYSSEVRHVAKVVKEKGGSLFLFTDS
                     ASCPVYSEADEVIRCITNSLLLTNSFAGLISILHVLVQMVYLDEKESAAMSRQNTILM
                     QESGYLVISNPKE"
     gene            1473..2162
                     /locus_tag="PK105_07085"
     CDS             1473..2162
                     /locus_tag="PK105_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61515.1"
                     /translation="MKRFLLLFAILTVFAIVPAFAQTEAELKAAYENAVKLAASAPKD
                     YTLNWQAARAARSYGDYLVVHKTTSWKETARAAAKEGMKYGEIAFNLNPSGIEGWYYY
                     GLCVGTYSDCVSVLTALTEGLKGKTQMGFENAYKFDKTYDNGGPILALGRFWQVLPGI
                     AGQDRKKAEKLFDEYIALFGSSPKANSDAWYFRGELYKDTKRPDLAKADLEKASSMGN
                     ENAKRLLAEMK"
     gene            complement(2444..2938)
                     /locus_tag="PK105_07090"
     CDS             complement(2444..2938)
                     /locus_tag="PK105_07090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU61516.1"
                     /translation="MNFSIRAIAPIEAETYYSLVNEVKDEGKYLFSTIRIPLDNMKKY
                     LAQHNGSSNPVLGAFDDKGTLLGWIDINISNIEELMHIGTIAMGVRRIHRGQGIGSAL
                     LDACISQARMLGLEKLELEVFASNSVARALYKKKGFFEEGIRLKKRKFEGHYDDLVCM
                     GLFL"
     gene            complement(3003..3989)
                     /locus_tag="PK105_07095"
     CDS             complement(3003..3989)
                     /locus_tag="PK105_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61517.1"
                     /translation="MNKAPTKDHRSKSNIVLGRDFGVLIAAILIAVFFTIASPVFLTA
                     YNFFNLLRQTAQLGIIAMAMTMLIISGEFDLSVGAIYAVTGVLTGILVKNMGLNIWLA
                     AFIGFLAALVLGFVNGTLISITKINSFIGTLSTQMIFRGIAMVLSQGQPISFLQSSLF
                     FDAFGRAEIFGNIPVQILWLFAWTLISFYLLHRTSFGVKVFATGDNRLAADLSGIKTT
                     RVKKTCFIITSAAAGLSSLISLAFLKTVTPTQGSGLELEAIAAGVIGGTSMSGGVGSI
                     LGTFIGTFIMAEVRTGLVLMGTDAYVQDAFVGLVIALAVIINVRFSISKGRS"
     gene            complement(4008..5534)
                     /locus_tag="PK105_07100"
     CDS             complement(4008..5534)
                     /locus_tag="PK105_07100"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU61518.1"
                     /translation="MRHRLLDEKESVLQSLLSVQNISKHFGNIHALRNVSLDISPKKI
                     IGLAGENGAGKSTLVRIICGAIPCDSGEMKYQGRPYKPHDVSDAESLGISVFHQEIPI
                     CPHLSIAANVFLGPTIPSLRIRPDWKFMNNRCIELYKKFLDEDIDPTIPIGECSAAEQ
                     QLALLVRVLSRNAKLVILDEPTTALSAPEVERLFDSIKRLRDEQGIGFIFVSHLLDEL
                     MELSDEVFVLRDGENVGHLYKAEFSAKELSRLIAGRSIDVQHRSAKGFAEAEITLEAR
                     SVSLSKSYEDISFSVHKGEVFGIAGLQGSGRSEVISSLFGLPPPDKGSVIIQGNDLGR
                     ITISKAITAGVGYLPEDRKTKGIFPDLNIAKNIGIVSIAQSRDLTSYNEEEYIELVDT
                     MASKLSIKISNPKDYISTLSGGNQQKAMLARWLALSPSILVLNEPTRGVDIGAKSEIA
                     KIISDMAEAGCTIIIASSEMEELLLMCDRIMVMNRGRARIILEKESLTRENLFFYAMS
                     "
     gene            complement(5584..6531)
                     /locus_tag="PK105_07105"
     CDS             complement(5584..6531)
                     /locus_tag="PK105_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU61519.1"
                     /translation="MKRILSVFIVLLLIAGAIGTVGAQAQKRYKFVVITHATAVPFFV
                     PVRKGAEDAGKLVGADVVYTGPTGFDIQQHVEFVKSAIAQKVDGIAMTMPDPNAFNAV
                     VKEAMSKGIPVIAINADAPESGRLAYIGQGNYEAGRSMGMQIVKLLPNGGDVLLTIHT
                     AGAENLEDRIRGIKDILDAQGGKFKYRVVATGTDMVRAVSLISSALQANPNIKGMFGV
                     EEVTGSAIAQIIERDRLKGKVMAGGFDLVTDELVAIQKGNMQFTIDQQPYLQGFQGVM
                     ELYLLKKFNLTPCDINTGIAPVTAENVNEVMKLAAEGYR"
     gene            complement(6886..>7101)
                     /locus_tag="PK105_07110"
     CDS             complement(6886..>7101)
                     /locus_tag="PK105_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61520.1"
                     /translation="LPGQHCFWPILRSYHIENLIIAPDYRNACAKLLLPCGQEFFDML
                     YCHAIIIIEIANLMQASSMHSLAKLCR"
BASE COUNT         2054 a         1649 c         1378 g         2020 t
ORIGIN      
        1 gcttggagaa gggtaccaat aagctcctgt tagcatgaaa gccgtcatgc cttccgtatt
       61 gaaagccttg gtttttgcat agagatcttc gagcgaccga gttagagagt ctgttcccaa
      121 gagccctata gctgtagtaa caccgtttag aaaattcatt gatatctgaa gttctggagt
      181 tctggtttca aatcccccct cgcctccgcc gcctataaaa tgctggtgcc catctatcaa
      241 tcctggaacg acgatacatc ccgatgcatc tatcgtttcg acatccggaa tcgaatcgga
      301 taaatcgata ttagaaccaa ttgagataat cctgtcgctt ccaatcaaaa tatccctgga
      361 tccaagcgga cagggtgcat atacttctcc atttttcagc aataacatgc tcatcttgtg
      421 ctccataatt tgcattggat ttattattct ttcggattac tgataaccag gtacccgctt
      481 tcttgcatca atatggtgtt ctgcctactc atagccgcgc tttccttttc atctaggtac
      541 accatttgca ccagtacatg taaaatcgat ataagcccgg caaaagaatt ggtcaacaat
      601 agcgaattag tgatacaacg tatgacctca tctgcttccg aatatacagg acatgaagct
      661 gaatcagtaa aaagaaacag cgaaccgccc ttttctttta ctacttttgc cacatgcctt
      721 acttccgacg agtatggagg aaatgtcatt agaaacataa catcttcgct cgaaaaagat
      781 ttgagcaccg gagcgtaatc cgagaccgag cccgaaacta catatacttc tttttctaaa
      841 gaactcatca aagtatattt gaaaatatgt gccacatagg cggttgctcc aaatgcactc
      901 aggtataccc tgcgagcctt ctttatgcct tctcccatta tctccagatc agcgatcttt
      961 atattggcaa aagtgctgcg ggtgttattg tattcattct ggaataactt ttgcagacgt
     1021 ttttcccctg gcaagatatc cagttcgctc agagcaatct tgtcataagg attcaatctg
     1081 tgccttattt catcttgaat ttcatgcttg aattcctgat atccttgaaa ccccaagctt
     1141 tttgcgaatc ggacaagagt cgcgctgctt acgccaatgg acttgcttat cgagtggatt
     1201 gacgaaaaag caacactctg ttcgtgatcc agcatataag agccgatctt ctgatagcta
     1261 ttggccattt caggaagcat tttcctgatc ttatcaaaaa ccatgcgttt cattttctcc
     1321 agatctaatc ttggtaactt ttgtttcggg attatcgcac cattaaattt atatgtcaac
     1381 gtttatcggc aagttttttc aattttttgc agttaaggac tcttgaccgc taactcacag
     1441 caggatagct ttttcacaag gaggcatctt tgatgaaacg ctttttgcta ttattcgcaa
     1501 tcctaactgt cttcgccatt gtccccgctt ttgcacaaac cgaagccgaa cttaaggctg
     1561 cttacgaaaa cgccgtcaaa ctagccgctt ccgcccccaa ggattacacc ttgaattggc
     1621 aagctgcccg cgccgcgcga tcttatggtg attatctcgt tgtacacaaa actaccagct
     1681 ggaaagaaac cgctcgcgcg gcagctaaag aaggcatgaa atatggggag atcgccttta
     1741 atctgaaccc gagcggcatc gaaggatggt actactatgg cctttgcgtg ggaacctact
     1801 cggattgcgt cagtgtactt acagccttga ccgaagggct aaaaggcaag acgcaaatgg
     1861 gctttgagaa tgcctataaa ttcgataaga cctacgataa tggtggtcca attctggcat
     1921 taggtcgctt ttggcaggtc ttgccgggaa ttgcaggcca ggatcgcaag aaagccgaaa
     1981 aactattcga tgagtatatt gccttatttg gatcctctcc taaggccaat agcgatgcct
     2041 ggtatttccg cggtgagctc tacaaagaca ctaagcgccc agacttggcc aaggccgatc
     2101 tggagaaagc atcctcgatg ggcaatgaaa acgcaaagag actgctagcg gagatgaaat
     2161 aatgttaggg gcccagtttt agggcaatat ctctaacaca aaacgaattc cacaataata
     2221 agactggctg gaaagaacta tctgtacttt attagatgcc aggagcccta accattaaat
     2281 cgatttgtat ttcgccgaag atcatagaga agccgctcat tatgccagac aaaagggcat
     2341 tctccaagtt atggaaaagg tcgaatggca ttttgcatca agtatagcag aaggctcgca
     2401 gaactcattt ttcacaaagg tatttatgca gaaggattta tctctataga aacagaccca
     2461 tacagaccaa atcatcgtaa tgtccttcga actttcgctt cttgagacga ataccttctt
     2521 cgaaaaagcc ttttttctta taaagagcgc gggcaactga atttgaggca aaaacctcaa
     2581 gttctaattt ttcgagacca agcattcttg cttgagaaat gcatgcatcg agaagagcac
     2641 ttccaatgcc ttgccctcta tggatccttc gaacacccat agcaatggta ccaatgtgca
     2701 taagctcctc gatattgctt atgtttatgt caatccagcc tagaagtgtt cccttatcat
     2761 caaaagcccc tagcaccgga ttggaagacc cattgtgttg ggcaaggtac ttcttcatat
     2821 tatcgagcgg aatgcgaatg gtcgaaaata ggtatttccc ttcatctttt acttcgttga
     2881 caagggaata ataagtctcc gcttctatcg gagctatagc gcgaatgcta aaattcatga
     2941 ttatcaactg ccgatattaa tcctcaatcg ggttcttttt caagtgttta gcattttgcg
     3001 gctcaggagc gacctttgga aatgctaaat ctcacattga taataacggc caaggcgata
     3061 actaatccta caaaggcatc ctgtacatag gcgtcagtac ccatcaaaac cagccctgtt
     3121 ctgacctcag ccattataaa tgtaccaatg aaggtcccca ggatacttcc aacccctccc
     3181 gacatcgaag ttcctccaat aactcccgcg gctatcgctt ccagttcaag accagagcct
     3241 tgagtcggcg taactgtttt tagaaaagcc agggatataa gcgaagataa acctgctgca
     3301 gcggatgtaa taataaagca tgtcttcttg acgcgagttg tctttattcc tgaaagatcg
     3361 gctgcaagcc ggttatcgcc tgtggcaaat actttcactc caaaagatgt cctgtgtaag
     3421 agataaaatg agatcaatgt ccatgcaaaa agccataaaa tctgcaccgg aatatttcca
     3481 aagatctcag ccctcccaaa ggcatcaaaa aacagagagc tctgtagaaa cgatattggt
     3541 tggccttgag atagcaccat agcaattcct cggaatatca tctgagttga gagagtcccg
     3601 ataaatgagt tgatttttgt aatggaaatg agagtaccat taacgaagcc aaggacaaga
     3661 gcagctagaa atccgataaa tgcagctaac caaatattca aacccatatt ctttaccagt
     3721 attccggtaa ggacccctgt cactgcatat attgctccta cagagagatc aaattcccca
     3781 gaaataatga gcattgtcat ggccatggca attatgccga gctgggcggt ctgtcgcaga
     3841 agattaaaaa aattatatgc agtaagaaag acgggcgaag caattgtaaa aaaaactgca
     3901 atcaatatag cagcaatcag aacaccaaaa tcgcgcccca atactatatt ggatttactt
     3961 ctatgatcct tggttggtgc tttgttcatg agcccttcct tttttattta actcattgca
     4021 taaaagaaaa gattttccct tgtcaaagat tccttttcaa ggataattct tgccctaccc
     4081 cgattcataa ccataattcg atcgcacata agcaaaagct cttccatttc tgacgatgct
     4141 attattatcg tacagcctgc ctctgccata tcggatatga tcttggcaat ctcgctctta
     4201 gccccaatat ctacgcctct ggtaggttca ttgagtacga gaatcgatgg agatagagca
     4261 agccaacgtg caagcatagc cttttgctga tttcctccgc tcaaggtaga tatataatct
     4321 ttaggattag aaatctttat agaaagcttg ctggccatag tatctactag ctctatatac
     4381 tcttcctcat tatagctagt tagatctctt gattgagcaa tactcacaat gccaatattc
     4441 tttgctatat ttaggtctgg gaaaatgccc ttggttttcc tatcttctgg taagtaacca
     4501 acacccgcgg taatcgcttt agatatagtt atcctcccaa gatcattacc ttggataatg
     4561 acagagcctt tatctggcgg cggcaatcca aaaagactcg aaatgacttc ggaccttccc
     4621 gatccctgta agcccgctat accaaagact tcacctttat ggacggaaaa agaaatatct
     4681 tcgtaagatt tcgataatga gacggatctt gcttcaagag taatctctgc ttcggcaaaa
     4741 ccctttgcgc tgcgatgttg cacatcgatt gatcttccag cgataagcct cgataattct
     4801 ttggctgaaa actcggcttt gtagagatgg cctacatttt ctccatctct tagaacaaaa
     4861 acctcatcgg atagctccat aagttcatcg agcaaatgac ttacaaatat gaaaccaatc
     4921 ccttgctcat ctctcagtct ttttatcgaa tcaaaaagtc tttctacttc tggcgcagac
     4981 aatgctgtag ttggctcatc gagaatcaca agctttgcat ttcgagacaa tactctgacc
     5041 agaagagcca attgctgctc ggcagcagaa cattctccaa ttggaatggt cggatcgata
     5101 tcttcgtcca ggaatttttt atacaattct atgcagcgat tattcatgaa tttccaatca
     5161 ggccttattc taagagaagg gatagttggc ccaagaaaca catttgccgc aattgaaaga
     5221 tgtggacaaa taggaatctc ttgatgaaaa acgcttatcc ccagagattc ggcatccgat
     5281 acatcatgag gcttatacgg tctaccttga tatttcattt ctcctgaatc acaaggaata
     5341 gccccgcaaa taattcggac aagcgtcgat ttgcccgcgc cattctcgcc ggccagaccg
     5401 ataatttttt ttggtgaaat atctaaagat acatttctca gagcatgaat attgccaaaa
     5461 tgctttgaga tattttgtac agataataga ctctgcaaaa cactctcctt ttcatcgagg
     5521 agacggtgcc tcaagacacc gtctccagat ggaagagcca atttttatga cgccaaatga
     5581 attctaacga tagccttcgg ctgcaagttt catgacttca tttacatttt cagccgtgac
     5641 aggtgcaata ccggtattta tatcgcatgg agtaagattg aacttcttga gaagatatag
     5701 ctccataaca ccctggaacc cctggagata cggttgctga tcaatagtaa actgcatatt
     5761 acctttttgg attgcaacaa gttcatctgt aacaagatca aaaccaccag ccataacctt
     5821 ccccttgagg cgatctctct cgatgatttg ggcaattgcg ctccctgtta cttcttccac
     5881 tccaaacatg cctttgatgt ttgggtttgc ttgcagggcg ctagaaatga gagagactgc
     5941 acgtaccata tcagtacctg tcgcgaccac tcgatatttg aacttaccgc cttgagcatc
     6001 gagtatatct ttgatgccgc gaatcctgtc ttcaagattc tctgcacccg cagtatgaat
     6061 tgttaaaagc acatctccac catttggcag cagttttacg atctgcattc ccattgatcg
     6121 cccagcttca tagtttcctt ggccaatata ggcgagcctt ccactttctg gggcatctgc
     6181 atttatggca ataacaggta taccttttga catcgcctct ttgactacgg cgttgaatgc
     6241 attcggatcg ggcattgtca tggcaatgcc atcaaccttc tgcgcaattg ctgattttac
     6301 aaattcaaca tgttgctgaa tatcaaaacc ggtcgggcca gtatatacaa catccgctcc
     6361 aacaagcttg cctgcatctt cagcaccttt tctaaccgga acaaaaaatg gaacagcggt
     6421 tgcatgggta ataaccacga atttataccg tttttgagcc tgagcaccga cggtacctat
     6481 tgctccagct ataagcaata gaactatgaa tactgagagt attcttttca taatgtcctc
     6541 ctctcgccct atgattttaa gatccccaaa acaggatcat attaatcaac cataaatcat
     6601 aagcatctaa ttaaatcaca gatgtcagga agacttgtta aatgcctaag aaccttcagt
     6661 ggctgataat ctaaaaacgt tttcagccat gtgacaattt tccttcaaga tgcataatct
     6721 gtcaagaaca aataagtatt tttgcaaaaa atcatcgcat actcggattg ctttcttata
     6781 aatccattat gcaccacttg ttgctttatc aattcatcga tctatcctga tcttgagttt
     6841 tgcctgcttt ttctattcct gatatagctt tgcaagaatc aacaatcatc tgcacaactt
     6901 tgcgagacta tgcatggatg aagcctgcat taggttggca atttcgatta tgattattgc
     6961 atgacaatac agcatatcga agaattcctg cccacatggc aggaggagtt tggcgcaagc
     7021 attacggtaa tcgggcgcaa ttatgagatt ctctatatga tatgaacgca aaatcggcca
     7081 aaaacaatgc tgaccgggga g
//
LOCUS       DAOOWY010000095         7071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_10672_length_7071_cov_11.260262, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000095 DAOOWY010000000
VERSION     DAOOWY010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10672_length_7071_cov_11.260262"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2608)
                     /locus_tag="PK105_07115"
     CDS             complement(<1..2608)
                     /locus_tag="PK105_07115"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HOU61521.1"
                     /translation="MSSHDAWKITEPGFSIDHIDTYETLFALANGNLGLRGDFDEKYP
                     AFHKGTYINGFFEKEPITYGEIAYGYAENHETILNIPNAKYIALFVDGEEFRLDAIEP
                     SRIEDYQRYLDIRNGVLVRIVRVRLSSNVLIEIRSTRLVSFLRESIAAIKYEVCLLET
                     PNRWPFVVRLESCIEGRVFNRRAEGDPRVGSKFQRDAYAIDRLEYDNSEARILITTQL
                     SRLAATAQIRHVFSGPRSMQVETAIEGSTVIHGWNGTVEAGQKFSLVKYMAYQMAALE
                     DAGSCWAEAHQELIRASQDGFEVLFNEQRSFLSEFWERADVVVEGDSKVQQALRFNLF
                     HVLQSTGRKGKTGLAAKGLTGEGYEGHYFWDTEIYVLPFLIYTMPEIARKLLEYRYSI
                     LDKARQRAKIMSEKGALFPWRTIDGEEVSAYYPAGTAQYHINADIIYAMEKYCKASND
                     IEFLYGPVAEVAAETARLWISLGHYSEDKRFRIDEVTGPDEYTVLVDNNCYTNLMAKN
                     NLAFAARVLKEMQENRSEAFEALRKKIGLLDSEIGFWESAAEAMYIPYDKKTGIYAQD
                     DSFFEKERWPFGETPADRYPLLLHYHPLVIYRHQVLKQPDLVLAQFLLSEKFSLAEKK
                     RNFKFYEPLTTGDSSLSHCIQSIMASETGDAKAALDYFMKTVRMDLDDVHGNSRDGIH
                     TAAMGGSWLSVVYGFAGFRDRASPGDEALFDSQAAAVDEAPSADRAVADVKAYYSFNP
                     RLPEGWQRLSFSLQLGSQRLNIDIKKDRVRYARETEFQARVQPSSEASREALAFKHKK
                     ELIVLKPGESVEISLKPVFRAAIFDLDGVVTDTARYHFQAWKKLCEEHGWQVDEALNE
                     KLKGVGRMESLDI"
     gene            complement(2612..3643)
                     /locus_tag="PK105_07120"
     CDS             complement(2612..3643)
                     /locus_tag="PK105_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU61522.1"
                     /translation="MKKQSVSASDVAKMAGVSRTTVSFILNNTQGKHISEATRQRVLK
                     AAEALQYVPDMNAISLAKKAHYSIGFFICYSRAIFSDAYLFRLIEGITLVLNKHHCRL
                     ELHSLRMTRRNYLDLVHSSKLDGLILMNTHTDDPGIKELEEAKIPFVVIGSIEGLSVP
                     QVDIDNVAAAREMVDYLVSLGHRKIAMIAHAPLEFYAAENRLEAYRQVLNENGIPIIS
                     KYIEVADFSEESGYRAMQRLLCLEDRPSAVFAGNDMVAYGAMQAIIDEGLSIPEDISI
                     AGFDDDFLSRYLNPSLTTVAQPSEGLGQAAAKLLIQEIISPRKSGLRERDHIVLPTMV
                     AKRESCRRI"
     gene            complement(3655..4527)
                     /locus_tag="PK105_07125"
     CDS             complement(3655..4527)
                     /locus_tag="PK105_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU61523.1"
                     /translation="MNKKGKISGNSSKKPLSNLLVHLILIVICVAWIIPSLGLFVSSF
                     RPRSEIALSGWWQAFQPPFAFTLENYSYVLTRGNMGRSFVNSLLIAIPSTLMPIFIAA
                     CAAYALAWMRFPLRKTIHLLIVALIVVPLQMTMIPILRLFNKIGLTGSFLGIWLAHTA
                     YGLPFAIYLLRNFIVQLPRSLIESAQIDGASHPIIFLRLVLPTSIPAISSLAIFQFMW
                     VWNDLLVALIYLGGTPNVAPMTVTISNMVNSYGGGWEYLTAAAFISMLLPLIVFFALQ
                     KYFVRGILAGSVKG"
     gene            complement(4520..5500)
                     /locus_tag="PK105_07130"
     CDS             complement(4520..5500)
                     /locus_tag="PK105_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU61524.1"
                     /translation="MHTNYILLAIIALAVIPIFLWVYLLISEWIASHTNRRAASILRP
                     WFWILPAISLLVVFLVYPVINTIVLSFMDSRSENFVGLKNYIYIFTDPNMLIVLRNNL
                     LWLVFFTAACLVLGLVIAVLSDRVRYESVAKAAIFLPMAISFVAAGVIWRFMYEYRPA
                     GSTQIGTVNAILTTLLPNFEPRAWLFDRSTNNAALIVVGIWMWTGFAMVILSAGLKGI
                     SVDVLEAARIDGAGEVAIFFKIILPLMMPTITVVATTLIINVLKVFDIVYVMTNGNLG
                     TEVIANRMYKEMFNYRNYGRASAIATLLLFAIIPVMIMNIKRFNAEEGNE"
     gene            complement(5600..6880)
                     /locus_tag="PK105_07135"
     CDS             complement(5600..6880)
                     /locus_tag="PK105_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61525.1"
                     /translation="MKRKTLILLLAIVSAGVALAAPASQKTINFLGVWGGQEAEVFNA
                     MAKQFEDKTGIKVELEATRDLDAVITTRVEAGNPPDIAGLPGPGKLIELAQAGKLVEL
                     SKILDMKEFDKNYSSGWKSLGTVDGKLYGVFMKAATKGLVWYNPKTFKDKDLPVPTNE
                     WTWDQMMALSKQAIAKGISPWSIGIESGAASGWVGTDWLENIFLRVNGPDKYRDWYEG
                     KLAWTSPEVKKVWEIWGQIVADPKMAYGGSRYINSTNFGNAASPLFTKPPQALLHQQA
                     SFIQGFIMDQFPTLKPVEDFDFVAFPAIDPRYSKAVEAAADVIAVFRDTPEVKAFVNY
                     LTSAEAQAFWAAGTGALATNKNVSLVFYSDPLIRRAADILNKSEIVVFDASDMMKSEM
                     NAAFWSAVVSYVENPKNLDSILAQLEKVRKEVYR"
BASE COUNT         1945 a         1756 c         1485 g         1885 t
ORIGIN      
        1 tgatatcgag cgattccatc ctgcccacgc ctttcagctt ttcgttcagc gcctcatcga
       61 cctgccagcc atgctcttcg caaagcttct tccatgcttg aaaatgatag cgcgccgtat
      121 ccgttactac gccatccaga tcgaagatcg ctgccctaaa gacaggcttg agcgagatct
      181 ccactgattc gccaggcttt agaacaataa gctctttctt atgtttgaaa gcgagcgcct
      241 ctctcgaggc ctcgctacta ggctgaaccc gcgcctggaa ttcagtttct cgcgcatagc
      301 gcaccctgtc tttttttatg tcgatattga gcctctggct cccaagctga agcgaaaatg
      361 agagcctttg ccagccctcg gggagccttg gattgaatga atagtacgct ttaacatctg
      421 caaccgcccg atcagcagaa ggtgcttcat caacagcggc cgcctgacta tcgaaaagcg
      481 cctcatctcc tggagaggct ctatcacgga atcccgcaaa cccatacaca accgaaagcc
      541 agctgcctcc catagctgcg gtatggatgc catctctgct atttccatgc acatcatcga
      601 gatccatgcg cacagtcttc ataaaatagt ccaacgcggc tttagcgtct ccggtttctg
      661 aggccataat gctttgaatg caatgagaga gcgacgaatc gcctgtggta agtggctcgt
      721 aaaacttgaa gtttcttttc ttctctgcaa ggctaaattt ttctgacaat agaaattgcg
      781 ccaacacgag gtcgggctgc ttgagtacct ggtgccgata tattacaaga ggatgataat
      841 gcaggagcag tggatatctg tccgctggcg tctctccaaa aggccagcgt tctttctcaa
      901 agaatgaatc atcttgagca taaataccag tctttttatc atagggtata tacatggctt
      961 cagcggcaga ctcccaaaat ccgatttcgc tatcgagcag ccctatcttt tttctcagcg
     1021 cctcgaaagc ctccgatcta ttctcttgca tttccttcaa gactcgcgct gcgaatgcaa
     1081 ggttgttctt ggccatgaga ttggtatagc aattattatc gacaagaact gtgtattcgt
     1141 caggaccggt tacttcatct attcgaaagc gcttatcttc ggaataatgc ccaagagaga
     1201 tccaaagccg tgcagtctct gcggctactt ccgctactgg tccatacaaa aactctatat
     1261 cgttgctagc cttacagtat ttttccatcg catatatgat atcagcattt atatggtact
     1321 gggccgtgcc agccggataa taggcactta cctcttctcc gtcgattgtg cgccagggaa
     1381 agagagctcc tttttcagac ataatcttag cccgctgtct ggccttatcg agaatagaat
     1441 atctgtattc gaggagcttc cgggcgatct ccggcatcgt atatatgaga aaaggaagaa
     1501 catatatttc ggtatcccag aaataatgac cttcatagcc ttctcccgtc aggccttttg
     1561 cggcaagacc ggtcttgcct tttctgccag tcgactgaag cacatgaaaa agattgaatc
     1621 tgagcgcctg ttgaacctta ctatctccct ctacaaccac atcagcgcgt tcccaaaatt
     1681 ccgaaagaaa gctccgctgc tcgttgaaaa gcacctcgaa gccatcctga gacgctcgaa
     1741 taagctcttg gtgagcctct gcccagcatg agcccgcgtc ctcaagcgca gccatctggt
     1801 atgccatgta ttttacaagc gaaaacttct gcccagcctc gaccgttcca ttccaaccat
     1861 ggattaccgt tgagccttcg attgcggttt caacttgcat gcttcttggt ccagaaaata
     1921 catgtctgat ttgagcggta gccgcaagtc tgctcaactg agtcgtgatg agaattctcg
     1981 cctccgaatt atcatactcg agtctatcga tggcataggc atcgcgctga aatttggagc
     2041 caacacgagg atcgccttca gctctcctgt taaaaaccct tccttctata caactctcta
     2101 gccgcactac aaatggccat ctatttgggg tttctaaaag acaaacctca tacttgatag
     2161 ctgctatgct ctcacgaaga aagctgacaa gacgcgtgga tcttatttcg atgagcacat
     2221 tgcttgaaag cctaactctt actatgcgta caagtactcc attgcgaata tcgagatatc
     2281 gttgatagtc ttcgatccgt gagggctcaa tcgcatcgag tcgaaattcc tcgccatcta
     2341 caaagagcgc aatgtacttg gcattgggaa tattcagaat tgtctcatgg ttttctgcgt
     2401 atccataagc tatttcacca taagttatcg gctctttttc gaaaaaacca ttgatatatg
     2461 tccccttatg gaacgcaggg tacttttcat cgaaatcgcc acgcaggcca agattgccat
     2521 tggcaagcgc aaatagagtc tcgtaagtat ctatatgatc aatactaaaa ccaggttctg
     2581 tgattttcca ggcatcgtgg ctggacatat ctcaaattct cctgcaggat tctcgttttg
     2641 caaccatggt ggggagaacg atatggtctc tctctcgcaa tccagacttc ctcggagata
     2701 ttatctcctg aatgagcagc tttgctgccg cttgccccaa accttcgctc ggttgcgcaa
     2761 ccgttgtcaa agaaggattg agatatctcg ataagaagtc atcgtcaaat cccgcgatag
     2821 agatatcctc aggaatggat aagccttcat ctatgatggc ttgcatagcc ccataggcaa
     2881 ccatgtcatt acctgcaaac accgcagaag gtctatcctc gaggcagagc aatctctgca
     2941 ttgctctgta cccagattcc tcggaaaaat ccgctacttc gatatatttt gaaataatag
     3001 gaattccatt ttcattcaat acttgccggt atgcttccag tctattttcc gcagcataga
     3061 actccaatgg cgcatgcgct atcattgcta tctttctatg gccgagcgag acaaggtaat
     3121 cgaccatctc tctcgcagcc gcaacattat cgatgtcgac ctgaggaaca gaaagaccct
     3181 ctatagagcc tattaccaca aaaggaatct ttgcttcttc caattccttt atgccaggat
     3241 cgtcggtgtg cgtattcata aggataagac catctagctt cgaggaatgt accagatcga
     3301 gatagttcct tcttgtcatt cgaagagaat gtagttccaa cctgcaatga tgtttattca
     3361 agaccaatgt gatgccttcg attagtcgaa agagataggc atcgctgaaa atagctcttg
     3421 aatagcatat aaagaaccct attgaataat gggctttttt ggccagactt atagcgttca
     3481 tatcggggac atattgaaga gcctccgctg cttttaagac acgttgcctt gttgcttcgc
     3541 ttatgtgctt gccctgagtg ttattgagaa tgaatgagac tgttgtccga gaaacgccag
     3601 ccatttttgc aacatccgat gccgaaaccg actgtttttt catatgaatg taccctatcc
     3661 cttgactgat ccggctagta tgccacgaac aaaatacttc tgcagtgcaa aaaaaacgat
     3721 aagcggcaac aacatcgaga taaaggctgc tgctgtcaaa tattcccagc ctccgccata
     3781 agaattaacc atgttgctta ttgttaccgt catgggcgca acgtttgggg ttcctccaag
     3841 atagattaga gcgactaaca agtcattcca aacccacatg aattgaaata tggccaacga
     3901 ggagattgcc ggaatcgacg tgggcaatac gagccttaag aatataattg gatgagaggc
     3961 cccgtctatc tgtgccgatt cgatcagaga tcggggaagc tgaacaataa agtttctcaa
     4021 gagatatatg gcaaaaggca aaccataggc agtgtgagca agccatatcc ccaaaaaact
     4081 gcccgtaagc cctatcttgt tgaataatcg aagaatcgga atcatcgtca tctgcagagg
     4141 aaccacgatc aatgccacaa tcagtagatg aatggtcttt ctcaaaggaa atcgcatcca
     4201 agcaagagca taagcagcac aagcggcgat gaaaatgggc atcagggttg agggaatggc
     4261 tatcagcaaa ctattcacaa aactcctgcc catatttcct cgtgtcagca cataagaata
     4321 gttctcaaga gtaaaagcga aagggggttg aaaggcttgc caccatccgc taagggcaat
     4381 ttcgcttcga ggtctaaatg aactgacaaa aagcccaagg gaaggaatta tccatgccac
     4441 acatattact atgagaatga gatgaacaag gagattgcta agcggctttt ttgatgaatt
     4501 ccctgaaatc tttccttttt tattcattcc cttcctctgc attgaagcgc ttaatattca
     4561 ttatcataac aggaattata gcaaaaagga gcaatgttgc tatggcgctt gctcttccat
     4621 agtttctata attgaacatt tccttataca ttctattggc gatcacttcg gttccaagat
     4681 tgccatttgt catgacatat acgatgtcga agacctttaa tacatttatt attagagtcg
     4741 ttgccaccac tgttatcgtg ggcatcataa gaggaagaat tatcttaaag aaaatcgcaa
     4801 cttcgccagc tccatctatc cttgcagcct caagcacatc tacgcttatc cccttaagcc
     4861 ccgcagagag aattaccata gcaaagcccg tccacatcca gataccaacc acaatgagag
     4921 cggcattgtt tgtagagcga tcgaacagcc atgcccttgg ttcaaaatta ggcaataggg
     4981 tagtcaatat ggcattaacc gtgcctatct gagtactgcc tgcgggtcga tattcataca
     5041 taaaccgcca tatcacgcct gctgcaacga aagaaatggc catagggaga aaaatcgcag
     5101 cttttgctac cgattcatat ctaaccctat cggatagaac cgcaatgaca agaccaagga
     5161 ctagacatgc tgccgtaaaa aacacaagcc agagtaaatt gtttcttagt actatgagca
     5221 tattaggatc agtgaatata tagatatagt tctttagacc cacgaagttt tcggaacgtg
     5281 aatccataaa actcaagacg attgtgttta ttacgggata cacaaggaat acaactagga
     5341 gagatattgc tggaagaatc cagaaccatg gacgtagaat agaagctgct cgcctgttcg
     5401 tatgtgaagc aatccattcg gaaatcaata aatataccca taagaaaatg ggtattaccg
     5461 caagcgcgat aatggccaat aaaatatagt tggtgtgcac gcccatacct ctactcacct
     5521 ggtccgacgc ttcgagaaag atgaaaagca ggggaagcct ttgtcccctg ctttccttta
     5581 catccagtcc aaacgaaatt tatctataca cttctttacg taccttttcg agctgagcca
     5641 gaatcgaatc gagattcttg gggttttcta catagctaac cacagcagac cagaatgcag
     5701 cgttcatctc tgacttcatc atgtccgagg catcaaatac gacaatctcg cttttgttca
     5761 aaatatctgc ggcccttctg attagcggat cagaatagaa caccagtgaa acattcttat
     5821 ttgtggccag cgctcccgtt ccagctgccc agaaagcttg tgcctctgca ctggtcaaat
     5881 agttcacaaa ggcttttact tcaggagtat ccctaaacac agcgattaca tcggcagctg
     5941 cttccaccgc ctttgaatac ctcggatcta tggccggaaa agccacaaaa tcgaagtctt
     6001 ctaccggctt tagcgtcggg aactgatcca taatgaatcc ctggataaaa ctcgcctgct
     6061 ggtgcaaaag cgcttgaggt ggcttagtaa atagcggtga agcggcattt ccgaaattcg
     6121 tcgaattgat atagcgagat ccaccatacg ccatctttgg atccgcaacg atctgacccc
     6181 aaatctccca cactttcttt acttcaggac tggtccatgc caatttgcct tcataccaat
     6241 cgcgatattt atccggtcca tttacgcgca agaaaatgtt ctcgagccag tcggtgccga
     6301 cccaacccga tgcagctccg ctttcgatac caatcgacca aggcgagatg ccctttgcaa
     6361 tcgcttgctt cgagagcgcc atcatctgat cccaggtcca ttcattggtt ggtacaggaa
     6421 ggtccttatc tttgaatgtc tttggattgt accaaacaag gcctttggtt gcagccttca
     6481 taaagacgcc atagagcttg ccgtccaccg tccccaggct tttccatcca gaggaatagt
     6541 tcttgtcgaa ttccttcata tccagaattt tggatagctc gacaagtttt ccggcctgag
     6601 cgagctcgat gagtttgcca ggaccaggaa gacccgcgat atcaggagga ttacctgcct
     6661 ccaccctagt tgtgataact gcatctagat ctcgtgttgc ttctagctcg acctttatgc
     6721 cagtcttatc ttcgaactgt ttcgccatgg cattgaatac ttcggcttcc tgaccacccc
     6781 aaacaccaag gaaattgatt gtcttctggc ttgccggcgc tgccagggct acccctgcgc
     6841 taactattgc cagcaacaga atcaaagtct ttcttttcat gaagaacctc cttgaatcta
     6901 tgcgttacta acgctattta gtaacattat aagcctagtt tccaaaacgc gcaaggaaaa
     6961 tttaagaaat tgagcttatg cgaccttggg tacaagtcag gaatgagttg acgcacaggg
     7021 ctagggaaac cagagggtgg tgaaggtagg taataatgat gattaaataa a
//
LOCUS       DAOOWY010000096         6939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_10969_length_6939_cov_11.081639, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000096 DAOOWY010000000
VERSION     DAOOWY010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10969_length_6939_cov_11.081639"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK105_07140"
     CDS             <1..514
                     /locus_tag="PK105_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61526.1"
                     /translation="SVPPKSQIELLALLGGGLALSELAEDTPLTLREAVIASSEFLTH
                     NLIFRSFEQRIQKALGLDVLYLQSSFIQRWLLDITDQTRENQTNLSRYLIGTELFGGK
                     YITDSAFVHFSLMIEQDPLEKTGFLQLNPKVGLELQSPFGLLQWSMSFGKEGMPLNNQ
                     ELSLSWRINF"
     gene            533..3085
                     /gene="bamA"
                     /locus_tag="PK105_07145"
     CDS             533..3085
                     /gene="bamA"
                     /locus_tag="PK105_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOU61527.1"
                     /translation="MRYSHKHFIVIIMIFCSFQIILAQTSTQELAGQDYSQDWFWNKK
                     IAGFRWDGLHYANRNDLDSMLRDYIGTLFTENIWIEIQAKLYALDWFESIESMALPAG
                     EEEDKIIIKFIVIEKPSINSIRVIGNSSIRTSELLDVASSKTGAIFNEDKAKADSLAM
                     QKLYLQKGFPDARVEYEKTEVEGDSNLIILTFKVTEGTAVVVKKIAFSGISAFSSQTL
                     KGQVTLKEAGLFQKGAFEESKLAESRTALEDYYASKGYIDAKVIDVIKNYSKDEKSQK
                     NYLDITFVIAEGKQWKLGDIFFEGNSIFSTSRLASLISLKQNEPINLKRLLTDKQKID
                     DLYYESGYIFNTITMTEIRDEQGGRISFKISITERDRAHIESISFKGNTKTKDYVIAR
                     ELPLEAGEVFSKAKIIDGLRNLYSLQYFSSIEPEIHQGSVVDLMDLVINVEEMSTAQI
                     QFGLTLSGLGQPSAFPISGFIKFSDLNLGGTGKNLQVNMTLSPDEQSLETSFGQSWLF
                     NKRISQSISFGIEHSVLQTAQDCIAPIFSEEDIPDPFIGLGSGSDEWDGLLSSVPDAY
                     LMDYDDYSFSLGYSMGYVHKIPQGDVGIAGGVSSGLDMSSYDTAKYRPYEKEMRDYNG
                     QWVLKDSIFGRVYLNRLDYWYNPGKGYFLSQRLTYTGILPMERQFYIRSDTRAEFYAT
                     LFSIPFSSSWIFSPVIGIHSGVQALLPQPWNSLAVTKDWVYIDGTFNVRGWKSLYKGH
                     GILVWENWLELHLPIVPQVLSFDGFLDAGAMMTENGWLDMTLDSPVSKGSNALEWNNF
                     AFSIGFGARFMIPQFPFRFYLAKRFVYDGSKVEWKTREGSFDFVLSITQPLY"
     gene            3165..5750
                     /gene="mutS"
                     /locus_tag="PK105_07150"
     CDS             3165..5750
                     /gene="mutS"
                     /locus_tag="PK105_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU61528.1"
                     /translation="MSKNPDSMLDQYRRIKAKHRDEILFFRLGDFYEMFNEDAKEASA
                     ILGLTLTKRQGLPMCGIPYHAAKTYIARLLKAGKKIAICEQKSLSNKRGLLDREVVEV
                     ITPGTALEDDYLEQGTNNYLADICIIDQRLCFAYLDVSTGEFRAFSLTSNKEMITETI
                     RAELYRLAPREILVQQSILDQPKFYQVIRECSAIIEAQPDWVYDLVHSLDLLKKKFGV
                     VSLKGFGFEDNAPEIAAAGNLLEYVQEMIRQDCPHIRALLPYEEREFVLLDEATKRNL
                     ELMRNLSDSSKRDTLIEVIDKTKTAGGARLLRQWLFQPLRIREKIEARLEAISFLYHN
                     QILLADIRKLLYGILDIERLISRLAVDKAHARDLRAIRDSIDLGLDLFTLIESAKAPL
                     ELGPRMSMEQIDACRQVIDIIEKTILDDPSILLTEGNLIRDGYDKELDNLRNLHKNTQ
                     EILEDYLKDEKRQTGIQNLRIRYNKIIGYYIEVTKGNIANVPSHFVRRQSLVGGERYT
                     TDRLAELESKINGAQERIIEIEKNVFLKTRDSLKQFIPALLECASEISRIDCLASLAQ
                     TATEQGFTKPLITEDEDIEIVNGRHPVVEICLPYGDFVPNSIALSRKNNWFVLITGPN
                     MAGKSTVLRQTALIVLLAHMGSFVPAESAKIGLVDKIFCRVGAQDNLARGESTFLVEM
                     HETAFILNTATEKSLVIMDEVGRGTGTLDGVSIAWAVSEYLTSRISCRTLFATHYHEL
                     TTMAIPGIRNMRMVAIEQDGNISFPKRLEDGASSGSYGIHVAKLAGLPKEVVIRASQV
                     ESHFNALEQGLKEEKPKEDSTPSLELFSNEDIVIETLRNIDPDNITPIKALQILSELV
                     EILKQ"
     gene            5981..6496
                     /locus_tag="PK105_07155"
     CDS             5981..6496
                     /locus_tag="PK105_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61529.1"
                     /translation="MDCRNAQTLLAGIIPVFSFRGNAASLIHKFKTGQRISLAHYFAY
                     RISMLDELTADVASSTCLVPVPPRPEKLRSGQLDQVGAIAESLINFGFHYEKMLLRLP
                     GSSQQKMLDRAGRLKNASQSYALLSNKKTIAKIILLDDVCTTGATLEACAKLLMENGN
                     KVVSAIVLAAD"
BASE COUNT         2084 a         1340 c         1560 g         1955 t
ORIGIN      
        1 ttcggtgcct ccaaaaagcc aaatcgagct tcttgctctt cttggtggtg gattggcatt
       61 gtcggaatta gcggaagaca ctcctcttac attgagggaa gccgtaatcg caagttccga
      121 gtttcttaca cataatttga tctttagatc attcgaacaa cgcatacaga aggctttagg
      181 cctcgatgtt ctctacctgc agtcttcctt tatccagcga tggcttctcg atattacgga
      241 ccagacccgt gagaatcaga cgaatttatc tagatatcta attggcacag agctttttgg
      301 aggaaaatat ataaccgatt cagcttttgt gcatttttct cttatgatag agcaggaccc
      361 gcttgagaaa actggatttt tgcaattaaa tccaaaagtt gggcttgaac tgcaatctcc
      421 atttggtctt ttgcaatgga gtatgtcctt tggtaaggaa ggaatgccgc taaataatca
      481 agagctcagc ttgagctggc gcataaattt ttgagtaact acaggagcag caatgagata
      541 ttcgcacaag catttcatcg taatcataat gatattttgt tcttttcaaa taattctcgc
      601 tcagacctca acacaagagc ttgctggtca agattattct caggattggt tttggaataa
      661 gaaaatcgct ggatttagat gggatggatt acactatgcg aaccgcaatg atctagattc
      721 aatgctgcgc gattacatcg gcaccctttt tacagagaat atttggatag agattcaagc
      781 aaaactctat gcacttgatt ggtttgagag catcgaatcg atggcgttgc cggctggaga
      841 ggaagaagat aaaataataa tcaaattcat agtaatcgaa aagccatcta ttaactcaat
      901 aagggttatt ggaaatagtt ctatcaggac ctcggaattg ctcgacgttg cttcttcgaa
      961 aaccggagcg atttttaatg aagataaagc aaaagccgat tcgcttgcga tgcaaaaact
     1021 ctatcttcag aaaggttttc cagatgcaag agtggaatat gaaaagactg aggttgaagg
     1081 agactccaat ttaatcatat tgacatttaa ggtaaccgaa ggaacagcgg ttgttgtaaa
     1141 aaagatcgca ttctcaggta tttccgcatt ttcttctcag actctcaagg gccaggtaac
     1201 gcttaaagaa gctgggcttt ttcagaaagg agcattcgaa gaatcaaagc tggctgaaag
     1261 tcggacagct ttagaggatt attatgcgtc taaaggatat atagatgcca aagttataga
     1321 tgttattaag aattattcaa aagatgaaaa aagtcagaaa aactatttag atatcacttt
     1381 tgttatagcc gaaggcaagc aatggaaatt aggtgatatc ttctttgaag gcaatagcat
     1441 attttctaca tctcgcctag cttcgctaat atctcttaaa cagaacgaac ccataaatct
     1501 aaagcgactt ctcaccgaca agcaaaaaat agacgatcta tattatgaat ctggatacat
     1561 tttcaacact ataacaatga ccgaaattcg agatgagcag gggggaagaa tctcattcaa
     1621 gatctcgata acggaaaggg acagggctca tattgagagt atttccttca agggcaatac
     1681 aaagacaaag gactatgtta ttgcaagaga attaccactc gaagcaggcg aagtattctc
     1741 aaaagccaaa atcatcgatg gattgcgaaa tctatacagc cttcaatact tctcatcgat
     1801 cgagcctgag attcatcaag gaagcgtcgt tgatctgatg gatttagtca taaacgttga
     1861 ggagatgagt acggctcaaa tccagttcgg tcttaccctc tcggggctcg gtcagccatc
     1921 tgctttccct atctcgggat tcataaagtt tagcgatttg aatcttggag gcaccgggaa
     1981 gaaccttcag gtcaatatga ctctttctcc agatgaacag agtcttgaga cttcgttcgg
     2041 tcagagctgg cttttcaata agcgcatatc tcagagcatt agcttcggta tagaacattc
     2101 agtccttcag actgcgcagg attgcattgc tcctattttc tctgaggagg atattccgga
     2161 tccttttatt ggactcggaa gcggatcgga cgaatgggat ggcctgcttt cgtccgttcc
     2221 agatgcatac ttgatggatt acgatgatta ttcttttagt cttgggtatt ccatgggata
     2281 tgttcataaa ataccgcagg gcgatgtagg aattgccggg ggtgtttctt cgggcctgga
     2341 catgtcttcc tacgataccg ccaagtatcg tccatacgaa aaagaaatgc gcgactataa
     2401 tggtcaatgg gtattgaagg attccatttt tggccgtgta tatttgaatc gtctggacta
     2461 ctggtataat cccggaaagg gatacttttt gagccaacgt cttacctata cgggcattct
     2521 tccaatggag cgtcaattct atattagatc cgacacacgc gccgagtttt atgcaacact
     2581 attctctatt cctttttcta gttcctggat attctcacct gttatcggga ttcacagtgg
     2641 agttcaggcg cttttgcctc aaccatggaa ttcgctagca gttacgaaag actgggtata
     2701 tattgacggc actttcaatg tgagaggctg gaaatctcta tacaaagggc acggaatcct
     2761 tgtatgggaa aactggctgg agctccatct tcctattgtt ccgcaggttc tcagctttga
     2821 cggatttctc gacgcaggcg caatgatgac tgagaatggc tggctagata tgactcttga
     2881 ttcgccggtt tccaaaggga gtaatgcctt agaatggaat aattttgctt tttccattgg
     2941 ttttggagct cgatttatga ttcctcagtt tccttttaga ttctatcttg cgaagcgttt
     3001 cgtatatgat ggttccaaag ttgaatggaa gaccagagaa ggttccttcg attttgtttt
     3061 gagcattaca cagcctctat attgattcat ctcaaatttg tatagagcca aagacaataa
     3121 ggctgcttct tgtgctatta gaaccagcct tctataatgg tgccatgtca aaaaatccag
     3181 actcaatgct cgatcaatat cgcagaataa aggcaaagca tcgcgatgaa atactttttt
     3241 ttagattggg cgatttttac gaaatgttca atgaagatgc caaagaagct agtgccattt
     3301 taggtcttac tctcaccaag cggcaaggcc ttcctatgtg tggaattcct tatcatgccg
     3361 ctaaaaccta tatcgctcga ctactcaaag ccgggaaaaa gatagcaatt tgcgaacaga
     3421 aatcgctttc caataagcgc ggtcttttgg atcgtgaagt agtagaagtt attacgccag
     3481 gtactgcctt agaagacgat tatttggaac agggaacaaa taattatctg gcagatatat
     3541 gtataattga tcagaggctt tgttttgctt acctagatgt atccacaggt gaatttagag
     3601 ccttttctct gacttcaaat aaagaaatga taacggagac tattcgagca gaactatatc
     3661 ggctggcacc tcgcgagatc ctggttcaac aatccattct tgatcaaccc aagttttatc
     3721 aggtcatccg tgaatgcagc gcgatcattg aagcccaacc agactgggtg tatgatcttg
     3781 tccactcgct cgacctgctt aagaaaaaat tcggtgttgt atcactgaag ggttttggct
     3841 ttgaagataa tgctcctgaa atcgcggcag caggcaattt acttgaatat gtgcaggaaa
     3901 tgatacgcca agattgtcct catatccgtg ccttgttgcc ctatgaagaa agagaattcg
     3961 tgctcttgga tgaggctaca aagagaaacc tggaattgat gaggaatctt agcgattcta
     4021 gtaagagaga tactcttatc gaagttatag ataaaacgaa aacagcgggg ggagcccgtc
     4081 ttctgcggca atggctcttt caacctctca ggattcgcga aaaaatagag gcgcgcttag
     4141 aggcgatttc ctttttgtat cataatcaaa tattgctggc agatatacga aagctgcttt
     4201 atggaatcct cgatatagag agattgatat cgcgtcttgc tgtcgataag gctcatgcga
     4261 gagatctaag ggctattcgt gattcaatcg atttaggtct ggatcttttt actcttatag
     4321 aatctgctaa agcgccttta gagcttggtc caaggatgag catggagcaa attgacgcat
     4381 gcaggcaagt tatcgatatc atagaaaaga ctatcttgga tgatccatca attctgttga
     4441 ccgaaggaaa tctcataaga gatggctatg acaaggagct agataatctg aggaatctgc
     4501 ataaaaacac tcaggaaatt cttgaagact atcttaaaga tgagaaaagg caaacaggta
     4561 ttcaaaatct acggataaga tataacaaaa taatcggcta ttacattgaa gtgacaaaag
     4621 gaaacattgc taatgttcct tctcacttcg ttcgcagaca atcgttggtt gggggagagc
     4681 gatatactac cgatcgatta gcagaacttg agagcaaaat caatggggca caggagagaa
     4741 taatagaaat tgagaagaat gtttttctca aaacaagaga tagcctgaag caatttatcc
     4801 cagcactact agaatgcgct tcagaaatat caagaataga ctgcttggca tcacttgcac
     4861 agactgcgac agagcaaggc tttacaaaac ccctaattac tgaagatgaa gatatagaga
     4921 tagtaaatgg ccgacatccg gtagtagaaa tatgtctgcc ttatggcgat tttgtaccga
     4981 attcaatcgc cctttctcga aagaataact ggtttgtatt aataacaggt cctaatatgg
     5041 ctggcaaatc taccgtgctt cggcaaacag ccctcattgt gcttcttgct cacatgggca
     5101 gttttgtgcc tgctgaatca gcaaagatag ggctggtaga taagattttt tgtcgtgtcg
     5161 gagcccagga caatctagcc agaggggaga gcacttttct ggttgaaatg cacgaaacag
     5221 catttatcct caatactgcg accgagaaaa gccttgtaat catggatgaa gtgggccgag
     5281 gaacagggac tctggatgga gtttcgatag cttgggctgt aagtgagtat cttacaagta
     5341 gaatcagctg tagaacactc tttgcaacgc attatcatga acttacaacc atggcgattc
     5401 ctggtatccg aaacatgaga atggtagcaa tcgaacagga tggtaacata tcttttccaa
     5461 aaaggctcga ggatggtgct tcaagcggct cttatggtat acatgtcgcc aagcttgctg
     5521 gattgccaaa agaagttgtc ataagagcat cgcaggttga atcacatttt aatgctctag
     5581 agcaaggact aaaagaagaa aaacccaaag aagactctac cccttctttg gagctgttta
     5641 gtaacgagga tattgtcatc gagacattga ggaacataga tccagacaat attactccaa
     5701 ttaaggctct ccaaatcctg tccgaattgg ttgaaatatt gaagcaataa tattaaaaaa
     5761 gaacgaatat ttgataatgt actcgatctt cagaaagact aataatcgga ctctcgatgc
     5821 tatatttccg cagcattgcg tactatgtgg atcaattgtt ggagcggtac cttggtttcc
     5881 tgcaccattg tgtgcctctt gtgaaagaat cctagagttt atcagtggtc cgcgctgcaa
     5941 gcactgcgga agagagctca tcagcgagaa tgaattctgt atggactgcc gcaatgcgca
     6001 gactttgttg gctggcataa ttccagtatt cagttttcgt ggcaatgcag ccagtcttat
     6061 tcataagttt aaaacaggcc agcgcatttc cttggctcac tattttgctt atcgaatttc
     6121 gatgctcgat gaattgacag cagatgttgc tagttctaca tgccttgtac ctgtgccgcc
     6181 aaggcctgaa aagctgagat ctggtcaact tgatcaagta ggtgcgattg cagaatcact
     6241 cataaatttt ggttttcatt atgaaaaaat gcttttaaga ctgccaggaa gcagtcagca
     6301 aaaaatgctc gatcgtgctg gccgcctcaa aaatgcttca caatcttatg cgctcttatc
     6361 aaacaaaaaa accatagcaa agatcatttt gctggatgat gtttgcacga caggcgcaac
     6421 tcttgaagct tgtgcaaaac tattgatgga aaacggaaac aaagttgtga gtgctatagt
     6481 ccttgctgct gattaggctt atcgcgcaag agcgaatctt gacgaatgct aagagaatat
     6541 ttataatgac actataaaaa agtctgagag attctttttt gcggcatgca ggaagagtcg
     6601 tgcaaacggc tcttttttat tccctgtatt ttgggggaaa tagagcggct attgttgaac
     6661 acttaaaaag tgtttaaaga ttgctggcct gcaagtagga aatacgccaa aagcggtttc
     6721 tctctggata tgcgcttccg cttgagctta acaatcgttg acatagaagc acatcgccaa
     6781 aagaaggcaa gataaatagg cctgtctggc tggagtctga acgactatta agcctagcat
     6841 tatgcggaag aaaaaaggcg gatggaaggg gatgatagat aaggaacttc aagaagagat
     6901 cgatgcaaca ctaaaagcag gagggctcga tctattgga
//
LOCUS       DAOOWY010000097         6900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_11044_length_6900_cov_11.724324, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000097 DAOOWY010000000
VERSION     DAOOWY010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11044_length_6900_cov_11.724324"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..4204)
                     /gene="pgk"
                     /locus_tag="PK105_07160"
     CDS             complement(65..4204)
                     /gene="pgk"
                     /locus_tag="PK105_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU61530.1"
                     /translation="MTPDLKHPLLNTWENILAHLDRVCESDNEQTQIQLLIELLINLR
                     LIGTDSAIPDMRIQKSDIDDIYRQVEKKTRSMLSSVKAPSAKFIYYAREVIKKFPVRQ
                     DAEEMIERIRSDAEAFEESRTEDDPLRGFAGDLRKEVHRRLSPRIITHYLNPYIELAV
                     HRNPEPMVNAGYVALPYTFSPDDEDEQFIDLAIDLLKRLQFLWDYEEGDMATVRQNLR
                     NNLDIFERCFLRAEVEGLLGILNPESLSSADKELDAFKRLASVKFFLKESYLESRIDL
                     YDLILLDLGLGRLIFLLANDLTNNYFAEVSPRNIRDALEVVRELLAISSIKGLRIQNV
                     QLRQNELGELRESSVSDFIKLKHSLEAISSELQQYVQSEIIDEMTGSLNQILEDYKVP
                     TSKLSQIKTRFFNNFIRRTQIHVLSEFVEKVSTAVNNELERQQGEGQLYLRFQRLNDK
                     GSFSECIEEKGIDAYIAPTWRKPEAWLRPFLGGKGNSIIDMAQIGLRVPPAFVLSYPL
                     LAAMSRNTNHIRAGIIDKLRELEQRTGTMLGNGDKPLLLSVRSGALTSLPGMMSTIMN
                     IGLVPRVQAALSKEFGTDFVDTLYLRFLENCKSALDLSRQCPRTRYKDDVAQGAAQKT
                     KLLEDQGSGLCLQRKKMAELESFLVTFLGPSFLTDEEEQLFACIELVYSSRTSKTVDL
                     YSKTLASHETIETAVTIQQVVFGNLNENSLSGVVMTRNPITGEDKLFGEFKIRAQGEE
                     IVMGSLDTLPIEEICPEIASQLLECKRNLIEFYHQDLDIEFTVEDGVLYLLQARAARL
                     GSFAQLMAATDFLKRGIITLDKYRENIGSLELAYANIPLPRADFRFRQWVPPLATGIP
                     INGGVVSGTLIITIERLKEAEKRRESVIFFAVNTKPTDFNIFNLSSAIVTVYPGRTSH
                     AAITAMTLNKACIVGCSDIEIDYEHRKVIFHGAGDIALTEGERITIDGNAGAVYRGVA
                     PISDTCMPVSSIHEAILKAQSAGEAAQIVETMISTKMKALRRESSLRKKSLAEAGDLH
                     KRNVLVRVDANIDLSENSEPEVVDMRVSLMIPVIRDLLEAGATPIICSHRGDPGTHSL
                     QGISREQIYETYSLLPIAQKLESLMGSVVKFHHVSIGSSGLLISRKDIVPDRINMLEN
                     LRYATGEKDNDEAFARSLAELSDSIFVNDAFNVCLRRHASIVGVPRFIQTSIAGPTVL
                     QELMILEKVLNKVERPFIAVFSGDEIEAQYGAMASMLPRVDAMAMILPSNTASLAPFS
                     NSYPAKITVIDPKDPGWLNKSYALGALLENARTILWAGPAGLTSRLGKDLEDLPPYIV
                     SLHRAMGNASLTIICSETEKHFAGKTTENIHISTGPRSFLEYLERLSLPGIVELDDSM
                     R"
     gene            4342..5232
                     /locus_tag="PK105_07165"
     CDS             4342..5232
                     /locus_tag="PK105_07165"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOU61531.1"
                     /translation="MRVLNFGSINIDYVYSVDEFVKSGETKAARSLSVFAGGKGLNQS
                     LAMAKAGLRVIHAGKVGKEGEFLIDTLKNGGVDTKLVEISDVPTGHAIIQVDDRGRNC
                     ILLFGGANQDIGEAYIDRSLLDFGSGDMLILQNEISSISSIIAKAKTKGMTIVFNPSP
                     YSSLVPNYPLELVDVFIMNEIEATCLSGEQDPDRAAGLLVRSFPQALIVITLGEDGSM
                     CARKGEIIRQKAYRVRAVDTTAAGDTFSGYFIAGLVEGMEISKALDLAARAASLCITR
                     KGAADSVPWRKELEKWDFKS"
     gene            5241..6857
                     /locus_tag="PK105_07170"
     CDS             5241..6857
                     /locus_tag="PK105_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU61532.1"
                     /translation="MKSYSNSLALCVAFFMVCSIAGVSAQQAEPVQQPQQGEPSQLPQ
                     QPAPSQKDSLALAGEWRGTLKVGASSLRIVFHIVDSGTGYVATMDSPDQGARGIPVSG
                     VRIEGKGVVIEVASVGGSYTGTLDAVGNQLEGFWNQSGISFPLTLSRQPSAEIHTEQK
                     SMPMKEPASLAYRVKEVRFNGGAEEVQLAGTLTLPKVNGPFPAVVLVSGSGPQNRDEE
                     ILGHKPFFIIADYLTQRGIAVLRYDDRGVGASTGDYQAATTFDFAADAQAAIDFLARQ
                     EGIDENNIGIIGHSEGAIIASILAAREEISWDGWASANGAGHEDTCDTCGAAGFNETR
                     ESSRPAFIVLLAGPGVQGYELLLMQNEAIGRASGLSGEQIIEANNLNRKLYDIVIEER
                     DEQLLRKKLTDVFYEEIDLNPVLSPLDKEAQKAQVPQVIAPLLSPWIRTFLELDPADY
                     LRKVKVPVLALNGSKDLQVPCKSNLEAIRSALSEAGNNSSTFVELEGLNHLFQHATTG
                     LPSEYSEIEEDFAPEALQIMGDWILNIISP"
BASE COUNT         1749 a         1570 c         1748 g         1833 t
ORIGIN      
        1 agctatgtca aatataatta ggccaaagac ttctgtcaac gattaatgac gattcatgtc
       61 tttgctatct cattgagtca tcgagctcga caataccagg cagagaaagt cgttcaagat
      121 actccaggaa ggaacggggg cctgttgaga tgtggatatt ttcagttgtt ttccctgcga
      181 aatgcttttc cgtttccgaa cagataatgg tcaaggatgc atttcccatc gctcggtgca
      241 gcgagactat atagggagga agatcctcta agtcctttcc taatctggat gttaaaccag
      301 caggccccgc ccaaagaatg gttcgtgcgt tttcgagcaa agcacccaga gcgtagcttt
      361 tattaagcca gccaggatcc ttcggatcga ttacggttat cttagcgggg taggaattgg
      421 agaatggcgc cagggatgcc gtattactgg gaagaatcat tgccatggca tcgactcttg
      481 gaagcattga tgccattgcg ccgtattgcg cttcaatctc gtcgccactg aataccgcaa
      541 taaatggtcg ctcaacctta ttcagcactt tttcgaggat cataagctcc tgcaagacag
      601 tggggccagc aatactggtc tggataaaac gaggcacacc cacaatgctt gcatgccggc
      661 gcaggcatac attgaatgca tcattgacga atatgctatc gctcagctca gcaagagaac
      721 gagcaaaggc ttcgtcgttg tctttttctc ccgttgcata tcggagattt tccagcatat
      781 tgatccgatc tggaacgata tccttccttg agatcaataa gccggatgag ccaatgctca
      841 catggtgaaa tttgacgaca cttcccataa ggctttccag tttctgtgcg attggcagaa
      901 ggctgtatgt ctcgtaaatt tgttcgcggg atatgccttg caggctatgg gttcccggat
      961 ctccgcggtg tgagcagatg attggcgttg cgccagcctc caagaggtct ctgattacag
     1021 gaatcattag cgaaactcgc atatccacaa cttcgggctc ggagttctcg ctgaggtcga
     1081 tgtttgcgtc aacacgcacg agcacattgc gcttatgcag atctccggct tccgcaagac
     1141 ttttttttcg gagacttgat tctcgccgga gtgccttcat ctttgtagaa atcattgtct
     1201 cgacgatttg ggctgcttct ccggcagact gcgccttaag gatggcttca tggatgctgg
     1261 agacgggcat acatgtgtcg gagatgggag caacacctcg atacactgca cctgcattgc
     1321 catctatggt tattcgttct ccttcggtta aggcaatgtc tccagcacca tgaaagataa
     1381 ccttacggtg ttcataatcg atttcgatgt ccgagcaccc gacaatacag gccttattca
     1441 gcgtcattgc ggtgatggct gcatgcgatg tgcgccccgg atagaccgtt acaattgcac
     1501 ttgagagatt gaagatattg aaatccgtcg gttttgtatt gacagcaaag aaaatgacac
     1561 tttcccggcg cttctccgct tcctttagcc gctcgatagt tatgatgagt gtcccagaga
     1621 caaccccacc gttgatcggt atacccgttg ccaggggtgg aacccattgt ctgaacctaa
     1681 aatcggccct cggtaatgga atattagcgt acgcaagttc taagcttccg atgttttccc
     1741 tgtatttatc gagtgttatg attccacgtt tcaaaaagtc cgtggcagcc atcagttgtg
     1801 caaaggatcc aagtcttgcc gccctcgctt gcaggagata taagacacca tcctcgaccg
     1861 tgaattctat gtcgagatcc tgatgataga attcgatgag attccttttg cattccaaga
     1921 gctgactggc tatctccgga cagatctctt cgatcggtaa agtgtccagg gagcccatga
     1981 cgatttcttc cccttgagct ctgattttaa actcaccaaa gagcttgtcc tcaccggtga
     2041 tggggttgcg agtcatgact acaccactca ggctgttctc attaagattt ccaaaaacga
     2101 cctgctgaat agtcaccgcg gtttcgatag tctcatgtga ggcgagagtc ttggaatata
     2161 ggtcgacagt ctttgaagta cgcgagctgt aaacaagctc gatacaggca aaaagctgtt
     2221 cttcttcatc ggtcaaaaaa gaaggcccca aaaatgtaac caggaaagac tcgagttctg
     2281 ccatcttttt gcgctgtaga caaaggcctg agccctgatc ctcgagtagt ttagtcttct
     2341 gcgcagcgcc ttgagcgacg tcgtctttgt atcttgttct aggacattgc cggctgagat
     2401 ccagagcgct cttgcagttc tccagaaatc gtagatacag agtgtcgacg aaatctgtcc
     2461 caaattcttt ggatagtgcc gcttgtaccc ggggtacgag tccgatattc attattgtcg
     2521 acatcatgcc aggcagcgaa gtgagagctc cagatcgaac agacagaagc aagggtttgt
     2581 ccccattgcc taacatcgtg ccggttcttt gttcgagctc gcgcaactta tcaataattc
     2641 ctgctctgat atgattggtg tttcggctca tagcggcgag aagaggataa ctgagaacaa
     2701 aggctggcgg aacccgaagg ccgatctgcg ccatgtcgat gatactgttt cccttaccgc
     2761 ctagaaaggg gcgcagccaa gcctcgggct tgcgccaggt tggtgcaatg tatgcgtcga
     2821 ttcccttttc ttcgatgcac tcggagaagg aacccttgtc attcagacgc tgaaagcgca
     2881 gatatagttg gccctctccc tgctgtcgct cgagctcatt attgacagcg gtggagacct
     2941 tttcgacaaa ttcgcttagc acatggattt gggtacggcg aataaagttg ttgaaaaacc
     3001 gtgtttttat ctgagagagc ttagaggtcg gaaccttata atcctccaga atctgattga
     3061 ggctccccgt catctcatcg attatctcgg attggacata ttgctgtagt tcggaagaga
     3121 ttgcttcgag actgtgcttg agcttaataa aatcggagac cgacgattct cgcagctctc
     3181 caagttcgtt ttgtcgcaat tgtacatttt ggattctaag accttttata ctagaaattg
     3241 caagcaactc gcgtactacc tcaagcgcat cgcggatatt tctgggactg acttcggcaa
     3301 aatagttgtt ggtaaggtca tttgcaagaa gaaagatgag gcgtccgagc cctaaatcga
     3361 gcagaataag atcatagaga tcgattcgcg attccagata cgattccttc agaaagaatt
     3421 ttacgcttgc aaggcgctta aaagcatcga gctctttatc cgcactagaa agactttccg
     3481 gattcagaat accgaggagc ccttcgacct ctgcgcgaag aaaacagcgt tcgaagatat
     3541 cgaggttatt tcgcaaattc tgtctaactg ttgccatgtc tccttcttca taatcccaga
     3601 gaaattgcag ccgcttgagg agatcgatgg ccagatcgat aaactgttca tcttcatcgt
     3661 cgggcgaaaa tgtatatggg agagcgacgt atcccgcatt taccattggc tctggatttc
     3721 tatgcacggc tagttctata tatggattga ggtagtgagt gataatacgc ggagaaaggc
     3781 ggcgatgcac ttccttgcgc agatcgccgg caaagcccct gagaggatcg tcttcagtac
     3841 gactttcttc gaaggcctca gcatccgaac ggatccgttc gatcatttcc tctgcatcct
     3901 ggcgaacagg gaattttttt atcacctcgc gcgcgtaata gataaatttt gcggaaggag
     3961 ccttgacact ggaaagcatg ctgcgcgttt tcttttcaac ttgtctatag atatcatcta
     4021 tatcgctttt ttggattcgc atgtcaggaa tagcactgtc tgtgccaatg agccttaggt
     4081 ttatgagaag ctcgatgaga agctgtattt gcgtctgttc gttgtcgctt tcgcacactc
     4141 gatcgagatg agcgaggatg ttttcccatg tgttcaatag gggatgtttc agatcaggtg
     4201 tcatagttag agtatttgaa tgattctgac ggattgtcaa tgttcaaagt atgaattaag
     4261 acagggcgcg agatccgata tcagtacacg attcagagtc ttatgctatg atggatccag
     4321 caagtgcaca ggagcacata gatgagagtt ctcaattttg gttcgatcaa tatagattat
     4381 gtctacagtg tagatgaatt cgtcaagtcg ggtgagacaa aagccgcacg ttcgctttcg
     4441 gtttttgcag gtggcaaggg actaaatcaa tctcttgcaa tggctaaggc aggactaagg
     4501 gtaatacacg caggcaaagt tggaaaagaa ggggagtttc tgattgatac gctgaaaaat
     4561 ggtggcgtag acacaaaact tgtagagatt tctgatgtgc ctacgggaca tgcgattatt
     4621 caagtcgatg atcgaggcag gaattgcatt ctgttgtttg gtggggctaa tcaagatata
     4681 ggcgaagctt atattgaccg ttctcttttg gacttcggtt ccggtgatat gcttattctt
     4741 caaaacgaga tatcctccat atccagcata attgccaagg caaagaccaa aggcatgact
     4801 attgtcttca atccatcgcc atattcgagc cttgttccaa attatcctct cgagctagtg
     4861 gatgtcttca tcatgaatga gatcgaagcg acttgtctct cgggggagca ggatccagac
     4921 agggcagccg gacttcttgt gcgcagtttt ccgcaagcct tgattgtgat aacgctagga
     4981 gaagatggct cgatgtgcgc tcgtaaggga gagattatac gacagaaagc ttacagggtg
     5041 cgcgctgtag acacgacggc agctggtgac acgttttctg gctattttat agctggatta
     5101 gttgagggaa tggaaatttc aaaagctctt gatcttgcag ctcgcgctgc ttccctctgc
     5161 attacaagaa agggagccgc tgactctgtg ccatggcgca aagagcttga gaaatgggat
     5221 tttaagagtt agaggggaat atgaaatcgt attctaattc gttagcatta tgtgtggcat
     5281 ttttcatggt gtgcagcata gctggagtat ctgctcagca agccgagcca gttcagcagc
     5341 ctcagcaggg cgaaccatct cagctgcccc aacagcctgc cccatcccag aaggacagcc
     5401 tagctttagc gggcgaatgg agggggacgc tcaaggtcgg tgcatcgagc ttgagaatcg
     5461 tctttcatat cgtggattcg ggaactggct atgtggccac gatggacagc cccgatcagg
     5521 gagcaagggg cattccggtg tcaggggttc gcatagaggg caaaggcgtg gtgatcgagg
     5581 ttgcatctgt tggaggaagc tacacaggca cgttggatgc ggtaggtaat cagctcgaag
     5641 gcttctggaa tcaatctggg atatcctttc ctctcactct ctcgaggcag ccatccgcag
     5701 aaatccatac agaacaaaag tctatgccta tgaaagagcc ggcatcattg gcttaccgag
     5761 taaaggaagt cagattcaat ggaggagccg aagaagtaca acttgccggt acccttacac
     5821 tgcccaaagt aaacggacct ttccccgccg tggtacttgt ttcaggctct ggtccacaaa
     5881 atagagatga ggagattcta gggcataagc cttttttcat aatcgctgat tatctgacgc
     5941 agagaggcat tgcggtactt cgatacgatg atcgtggcgt gggagcttcc acaggtgact
     6001 atcaagcagc aacgacattt gattttgctg ctgatgctca ggcggccatc gattttctcg
     6061 cgcggcaaga aggtatcgat gagaacaata tcgggattat cggacacagt gagggcgcga
     6121 ttatcgcctc gattctcgct gcgcgagaag agattagttg ggatggctgg gcaagcgcca
     6181 atggtgcagg gcacgaagac acttgtgata catgtggggc tgctggattc aatgagactc
     6241 gtgagtcttc aagaccagct ttcatcgtgc tgcttgctgg tccaggagtt caaggttatg
     6301 agctccttct catgcagaat gaggcgattg gccgagcttc aggcttgagc ggagaacaga
     6361 tcatcgaagc aaacaatctc aatcgcaagc tctacgacat tgtaatcgag gagcgagacg
     6421 agcaattgct ccgcaaaaaa ctgactgatg ttttctacga agagatcgac ctgaatccgg
     6481 ttttatctcc cctggacaaa gaagcccaaa aagctcaggt tccccaagta atagcgccac
     6541 tattgtcgcc atggattcgc acttttcttg agttggatcc tgcggattac ctaagaaaag
     6601 tgaaagtgcc agtgcttgcg ctgaatggat cgaaagacct ccaagtgcct tgcaaatcaa
     6661 accttgaggc gattagatcg gctttatcag aggctgggaa caattcctct acttttgtag
     6721 agcttgaagg gctcaatcac ctctttcagc atgcaactac agggctaccc tcggaataca
     6781 gcgaaatcga ggaggatttt gcgcctgaag ccttgcaaat aatgggcgat tggatattga
     6841 atattattag tccctaaaaa gactctagta ggttttctca ataaccctgc ttcgaaagat
//
LOCUS       DAOOWY010000098         6808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_11238_length_6808_cov_10.782319, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000098 DAOOWY010000000
VERSION     DAOOWY010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11238_length_6808_cov_10.782319"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1673)
                     /locus_tag="PK105_07175"
     CDS             complement(6..1673)
                     /locus_tag="PK105_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOU61533.1"
                     /translation="MTDFNKLEQDFDAWNTKVEKANEKHPERKKQFVTGSNAPINRVY
                     TPLDQKDFDYAKQLGFPGEFPFTRGVQPTMYRGRLWTMRQYAGFGTAEESNKRYKYLL
                     EQGQTGLSVAFDLPTQIGYDSDHPLSEGEVGKVGVAIDSLEDMEVLFDGIPLDRVSTS
                     MTINAPASVLLAMYIAMAEKQGVSAEKLNGTIQNDILKEYIARGTYIFPPEPSMRLIT
                     NTFEYCSKNVPNWNTISISGYHIREAGATAVQEVAFTLADGIAYVEAAIKADLDIDEF
                     APRLSFFFNSHNDLFEEVAKFRAARRIWAYIMRERFGAKNPKSWMLRFHTQTAGCTLT
                     AQQPDNNIIRVTIQALAAVLGGTQSLHTNSRDEALALPTEEAVRIALRTQQIIAYESG
                     VAETVDPLAGSYYIESLTDRIEKEALQYLKLIDDMGGAVRAIEQGYIQKEIQDSAYQW
                     QMDVEKGERIIVGLNKFQVEENPPKGLLRVDPAVGERQKEKLSALRARRNNTKVDSAL
                     ASLRSAAKGTDNLMFPILDAVRAYATLGEICGVLREEFGEYQPNVMF"
     gene            complement(1721..2269)
                     /locus_tag="PK105_07180"
     CDS             complement(1721..2269)
                     /locus_tag="PK105_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU61534.1"
                     /translation="MRVEFRLSGSGGQGLLLAGIVLAEAAINEGKNAVQTQSYGPEAR
                     GGSSKAEVVISDSEIDYPKATEPDYLLALSNDAYKSYGVSMKKGIIIADESLNIDPPA
                     KVPVVRLPILETASNKLGRAVVANMVALGVLGGISGVASPETLKEAVRNRVPKGTEEL
                     NTEALEQGLLLAKQAKDQLGLI"
     gene            complement(2248..3114)
                     /locus_tag="PK105_07185"
     CDS             complement(2248..3114)
                     /locus_tag="PK105_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOU61535.1"
                     /translation="MAEFLSYFRRDRLPHIWCPGCGNGTVTGALVRAIDKLGLDKNNT
                     VIVSGIGCSSRSVGYLDFDTLHTTHGRAIAFATGVKLANPKLKVVVITGDGDCAAIGG
                     NHFIHAARRNIDITVIIINNDTYGMTNGQASPMTPRGMFGTTAPYGNIEYNFDLCELA
                     KAAGATYVARATTYHYVLLEDLIAKAIMHKGFSVVEALSQCPTYFGRKNKIGGAVETL
                     NWIKDQTINVKAAASLPQEKREGKILIGELYKSAKAEFTEQYLELIDRLREADPSYIR
                     NEGESGCELNSD"
     gene            complement(3114..4247)
                     /locus_tag="PK105_07190"
     CDS             complement(3114..4247)
                     /locus_tag="PK105_07190"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOU61536.1"
                     /translation="MAEIIKLMQGNEACTMAAIAAGLRFYAGYPITPSTEIAEMCSEE
                     LPKVGGRFIQMEDEIGSISACIGASLTGVKAMTATSGPGFSLMQEAIGYAGLCEIPVV
                     IIDVQRVGPSTGMPTSPAQGDVMQARWGTHGDHPIIALAPGNVRECFELTFRAFELSE
                     KYRVPVIVLTDEVIGHMREKVVLPDPSSYTIPMRPMPESPEGYKPYAADPNGGVPPMA
                     PFGMGYRWHCTGLTHDENGAPTSKPSVAEALTRRLYLKIENRTSELADYFAEQLEDCD
                     LAVVSFGSSAMASLSAVRRARNEGYKVGMLRLKTIWPFPTKTVKSLLAHAKKILVPEM
                     NLGQLALEIERSAGCGQPVIRLGKANGELFHPDEVFAAIKEAL"
     gene            complement(4240..4515)
                     /locus_tag="PK105_07195"
     CDS             complement(4240..4515)
                     /locus_tag="PK105_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU61537.1"
                     /translation="MAGPQKKFFHIVRAKYCKGCSICVALCPKKVLELKNEKVFAARP
                     DDCIGCRMCELRCPDFAIEVHPVEEKSKFEADVNLVGLGLNSEANHG"
     gene            4766..6502
                     /locus_tag="PK105_07200"
     CDS             4766..6502
                     /locus_tag="PK105_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61538.1"
                     /translation="MKYLKIRDLTLRDGQQSQFATRMNNEQVKAVLDDYRQSHFYAME
                     VWGGAVPDSVMRFLGEDPWDRLAIIKEGIGNASKLTALSRGRNLFGYNPYPDSVIEGF
                     CKNAIAAGVDIMRIFDALNDIDNMKSSIRFVKEAGGLADCAVCYTVDPHFRLSDRIKG
                     VFTGKKIPPKIFTVDYYVKKAKELASLGADIFTIKDMAGLITPFMAAELVKALKNELD
                     IPIDLHTHCTPGFGVASLLAAMVNGVDIVDTAILSFSGGPAAPAYEIIRIFADRLGFS
                     VDVDNAAVARIDKRLRQIRSELASFDQYKRLPPELDLSKDSLSPELSKLFDDALEAAV
                     GGKYQKSLDICQQIEKAFFYPEPDEIVRHAQIPGGMYTNMLTQLKEAKLEHLLNEVLR
                     LVPTVRLDAGLPPLVTPTSQIVGVQAVNCVISIHEGKDMYENVSKNFEDLILGQYGKT
                     PWPVDPEFRYKICGTREEVPYDTSKYKPQDNPPIPEAGGVPLALNEKEQLLLELFPSV
                     AAKFLRNKRIAEWQAAHPEGTPSKEQPGLGEQNLAPYFDPDYEPSDGEPPSFWEIAPS
                     WAGWTDSIALGQ"
     gene            complement(6532..>6808)
                     /locus_tag="PK105_07205"
     CDS             complement(6532..>6808)
                     /locus_tag="PK105_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOU61539.1"
                     /translation="GPRAMEESFGSVAHGAGRCMSRKAAARQIQFDALSDQMRAHSVI
                     LLAANKRLACEEAPLAYKNVNDVVETVSGAGLASLVARTKPLLVIKG"
BASE COUNT         1708 a         1725 c         1546 g         1829 t
ORIGIN      
        1 cccctttaga acatcacatt aggctgatat tcgccaaatt cctcgcggag cactccacat
       61 atctctccga gggtagcata ggcacgcact gcatcaagta ttgggaacat gagattatcc
      121 gttcccttag ctgcgcttct caaagaagca agggcagaat ccacttttgt attatttcgg
      181 cgcgctctaa gtgcggagag tttctctttc tggcgctctc cgactgctgg atccacgcgc
      241 aaaaggccct tgggtggatt ctcctcgact tgaaatttgt tgagtcctac gatgattcgc
      301 tcaccctttt cgacatccat ctgccattga tatgcagaat cctgaatctc cttctgaatg
      361 tatccttgct cgatagccct aaccgcccct cccatatcat ctatgagctt aaggtattgt
      421 agggcttcct tttctatgcg atctgtgagg ctctctatat agtatgagcc agccagtgga
      481 tccacggtct ccgctacgcc ggactcgtat gcgattatct gttgggtacg aagggctatt
      541 cgtaccgcct cttcggtggg aagagcaagc gcttcgtcgc gggaattggt atggagggat
      601 tgcgtaccgc ccagcacggc ggccaaagcc tgaatagtta cgcggattat gttgttgtcg
      661 ggctgctggg cggtgagggt gcagccggcg gtttgggtat ggaagcgaag catccagctt
      721 tttggattct tggcaccaaa gcgctcacgc atgatgtaag cccagatcct ccgagctgca
      781 cgaaatttag ccacttcctc gaaaagatca ttgtgcgaat tgaaaaagaa agacaagcga
      841 ggcgcaaatt cgtcgatatc caaatctgct tttatggcag cttcgacata ggctatgcca
      901 tctgccaggg taaatgctac ctcctgcacg gctgtcgcgc ctgcttctcg aatgtgatag
      961 ccagaaatag aaatggtatt ccagtttgga acattcttag agcaatattc gaatgtattt
     1021 gtgatgagcc tcatggaagg ctctggcggg aaaatatagg ttcctctggc tatatattct
     1081 ttcaagatat cattctgaat ggtgccattt agcttctcgg cactgactcc ctgcttctca
     1141 gccattgcaa tatacattgc cagaagcacg gaagcaggag catttattgt catgctggtc
     1201 gagactctgt cgagcgggat gccatcgaac aacacttcca tgtcttctaa cgaatcgatg
     1261 gccacaccca ctttccccac ctctccttca gacaatggat gatctgaatc ataaccaatc
     1321 tgagtaggca aatcgaaagc cacagaaagc cccgtttgac cctgctcaag caaatactta
     1381 tagcgcttat tagattcttc tgcggtacca aaacccgcat actgccgcat agtccataat
     1441 ctgcctctat acatggtcgg ttgaacaccc cgcgtaaagg ggaattcgcc cgggaacccc
     1501 agctgcttag cataatcgaa gtctttttga tcaagtggag tatatactcg attgattggg
     1561 gcattcgatc ctgttacaaa ttgcttcttg cgttcaggat gcttttcatt tgccttttca
     1621 acctttgtat tccatgcatc gaaatcctgt tcaagtttat taaagtcggt catgcttccc
     1681 tcctcgaggt caccaattta tgtcaaatgg atttcatata ttagatgagt ccaagctgat
     1741 cttttgcttg cttagccaac aagagccctt gttcgagcgc ctcagtgttt agctcttcgg
     1801 ttccttttgg cacacgattc cttacggctt cttttagcgt ctctggactt gctactccgg
     1861 agattccacc caatactccg agcgcaacca tgttggccac aactgccctt cccagtttgt
     1921 tagatgcagt ctcaagtatg gggagcctca caaccggcac cttagccggt ggatcgatgt
     1981 tcaagctctc atctgcaatg atgattcctt ttttcatcga gacaccatag cttttatatg
     2041 catcgttgga tagtgcaaga aggtagtcgg gctctgtggc tttaggataa tcgatctcgc
     2101 tatccgagat aacaacctcc gccttgctcg aaccaccacg ggcttccggt ccgtaactct
     2161 gtgtctgaac cgcattcttt ccttcgttga ttgccgcctc tgctaatacg atacctgcaa
     2221 gcaaaagccc ttgtcctcct gagccgctca gtctgaattc aactcgcatc ctgattctcc
     2281 ttcgtttcgg atatagctgg gatcggcttc ccttagtctg tcgatcagtt ctaagtattg
     2341 ctctgtgaac tcagccttcg cgctcttata tagctcgcca ataaggattt tgccttcgcg
     2401 tttttcttgt ggcaatgagg ctgcagcttt tacatttatt gtctggtcct taatccaatt
     2461 gagtgtttca acggcaccac cgatcttatt ctttcggcca aagtaggtcg gacattgtga
     2521 tagtgcctca accaccgaga aacctttatg catgatcgct ttggcaatca ggtcttcgag
     2581 taaaacatag tgatacgtgg tcgctcttgc tacataggtg gcaccagccg ccttagcgag
     2641 ctcgcacaga tcgaaattgt attcgatatt tccataagga gcagttgtcc cgaacatccc
     2701 gcgaggcgtc ataggagagg cttggccatt tgtcatgcca taggtatcgt tattgataat
     2761 aatcactgtt atatcgatat ttcttcgagc ggcatggata aaatgattgc cgccgattgc
     2821 ggcgcaatcg ccatccccag ttattaccac taccttgagc ttaggattcg ccagctttac
     2881 gcccgtagca aaagcaattg cgcgaccgtg cgttgtatga agcgtatcga aatcgagata
     2941 gccaaccgag cggcttgaac atccaatccc agatacaata accgtattgt tcttatcgag
     3001 cccaagttta tcgatcgcac gcacaagggc accggtaaca gtgccatttc cgcaaccagg
     3061 gcaccaaata tgtggaagtc gatctcgacg gaagtaagag agaaattcag ccattagagt
     3121 gcctccttga tcgctgcaaa gacttcatcc ggatgaaaaa gctcgccatt ggcctttcct
     3181 aacctgataa caggttgccc acacccagcc gatctctcaa tttcaagcgc aagttgccca
     3241 agattcatct cgggcaccag gattttcttt gcatgcgcta gcagagactt tacggtctta
     3301 gtggggaaag gccatatagt cttgagcctc agcatgccca ctttatagcc ttcattgcgg
     3361 gcacgcctaa ccgcagaaag agaagccatt gcggaagatc cgaatgacac aacagccaga
     3421 tcacaatcct ctagctgttc tgcaaaatag tctgccaact ctgaagttct attctcaatc
     3481 ttgagataaa gacggcgagt aagtgcttct gcaacggaag gcttactagt tggagcgcca
     3541 ttttcatcgt gagttaggcc tgtgcaatgc caacgataac ccattccaaa aggtgccatg
     3601 ggtggaacac ctccattcgg atcggccgca tatggcttat agccttccgg actctctggc
     3661 attggcctca ttggaattgt atagcttgat ggatcgggca gaacaacttt ttctcgcata
     3721 tgcccaatca cttcatctgt caaaactatt accgggactc gatacttctc cgaaagttcg
     3781 aaagcgcgga atgtaagctc gaaacactcc cttacattgc caggagccaa ggcaattatg
     3841 ggatgatctc cgtgggtgcc ccatctggcc tgcattacat ctccctgagc cgggcttgtt
     3901 ggcatgcccg tagaaggacc aactcgctgt acatcgatga taactacggg aatctcgcaa
     3961 aggcctgcat agcctatcgc ttcctgcatc agagaaaaac caggacccga agtagcggtc
     4021 atggctttta caccggttaa tgaagcaccg atacatgctg aaatcgaacc aatctcatct
     4081 tccatttgaa tgaatcttcc tcccactttt gggagttctt ccgagcacat ctctgctatc
     4141 tccgtggaag gagtaattgg atatccagca tagaatcgta gccccgccgc tatggcagcc
     4201 atggtgcacg cctcatttcc ttgcataagc ttaatgatct cagccatggt tcgcctccga
     4261 attcaagcca agtccaacca gattgacatc tgcttcgaat tttgattttt cttcgacagg
     4321 atgcacctca atggcaaaat cgggacatct gagttcacac attctgcatc cgatgcaatc
     4381 atccggcctg gcagcaaata ccttttcatt ttttagctct agtaccttct ttgggcagag
     4441 agccacacat atgctgcaac ctttgcagta ttttgctctg acgatgtgaa agaatttttt
     4501 ttgaggacca gccatttctt tgctcttgaa caataatcgc cgcactcagt ctaacagacc
     4561 gagcattgcc cctttagatg ctccttcaaa aagaatgaac tttataagga taaatataaa
     4621 caggaacgca ttacggcgtt tttttattct acagtttatt cacgattttg tcaaaatcta
     4681 tcacttgtgc cataaatcat atatattgcc gatacttgtc gcttatggcg atttgagata
     4741 caaatagact tcaggagctt ttgagatgaa atacttaaag attcgcgatt tgacacttcg
     4801 cgatggccaa cagtcacaat ttgccacacg catgaataat gagcaggtca aggccgtgct
     4861 agatgattat cgccagagtc atttttatgc aatggaggta tggggcggag ccgttcctga
     4921 ttctgtaatg agattcctcg gtgaagatcc atgggatagg ctcgctataa tcaaagaagg
     4981 aataggcaat gcctcaaaat tgacagcact ctcacgtggg cgcaatctct ttggatataa
     5041 tccctatcct gattcggtaa tcgaagggtt ttgcaagaat gccattgcgg cgggtgtaga
     5101 tatcatgaga atcttcgatg cgctcaacga tatagacaat atgaagtctt cgataaggtt
     5161 tgtaaaagaa gcgggtggtc tggctgattg cgctgtttgc tacaccgtcg atccgcattt
     5221 tagattgagc gacagaataa aaggcgtttt tactggaaag aaaataccgc ctaagatttt
     5281 cacagtagat tattatgtca agaaagcaaa ggagctggca tctctcggcg ctgacatttt
     5341 tacaatcaaa gatatggcgg gactgattac tcctttcatg gctgcagagc ttgtaaaagc
     5401 gctcaaaaat gagcttgata ttccgatcga tcttcatact cattgtactc caggctttgg
     5461 agtggcaagt ctgcttgcag ccatggtcaa tggagtcgac attgtcgata cggccatcct
     5521 gtctttctct ggagggcctg ctgctccagc ttatgagata atacgcattt ttgcagacag
     5581 gttgggcttc tccgtggatg tcgacaatgc tgctgtcgca aggatagaca agcgtcttag
     5641 acaaatccga tccgaacttg cgtccttcga tcagtacaag agactaccgc ccgaactcga
     5701 tctttccaag gatagcctat cgcccgagct ctccaagctc ttcgatgacg ctcttgaggc
     5761 tgccgttgga ggcaagtatc aaaaatctct cgacatttgc cagcaaatag aaaaggcctt
     5821 cttttaccct gaacccgatg agatcgtacg tcacgcgcag ataccaggcg gcatgtatac
     5881 caacatgctc actcagctca aggaagccaa gctggaacac ttactcaatg aagttcttcg
     5941 tcttgtgcca actgtgcggc tcgatgcagg cctgcccccg cttgtaacac ctaccagtca
     6001 gattgtgggt gtccaggcag tcaactgcgt cataagtatt cacgagggaa aggacatgta
     6061 cgaaaacgta tcgaagaatt tcgaagacct tatcctcggt caatatggaa agacgccatg
     6121 gccagtcgat cctgaattcc gctacaagat ctgtggcaca agagaagaag ttccttatga
     6181 cacaagcaag tacaagcctc aggacaatcc accgattccg gaggcaggtg gagttccgct
     6241 ggcattgaat gaaaaagaac aactgcttct agagctattc ccctcggtgg cggcaaagtt
     6301 cctgaggaac aaaaggattg ctgaatggca agcggcgcat ccagaaggca ccccttctaa
     6361 agaacaaccc gggcttgggg aacagaatct cgctccatac ttcgatcctg attatgagcc
     6421 aagcgatgga gagcctccat ccttctggga aattgcaccg agctgggcag gatggacgga
     6481 ttctatcgcc ttagggcagt agaaagcgcc ttatgctctc cttatgatct tctagccctt
     6541 tataaccaag aggggcttgg tacgtgccac aaggcttgca agaccagcgc cgctaactgt
     6601 ttccacaaca tcgttgacat ttttataggc aagaggagct tcttcgcaag ccaatcgttt
     6661 atttgctgca agcaatatta cggaatgcgc tcgcatttgg tctgaaagcg catcgaactg
     6721 gatctgacgc gcggcagcct tgcggctcat acaccgacct gccccgtgtg caacgcttcc
     6781 aaagctctct tccatggctc taggcccg
//
LOCUS       DAOOWY010000099         6591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_11760_length_6591_cov_13.012699, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000099 DAOOWY010000000
VERSION     DAOOWY010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11760_length_6591_cov_13.012699"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1703
                     /locus_tag="PK105_07210"
     CDS             <1..>1703
                     /locus_tag="PK105_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61540.1"
                     /translation="AIKFGLDSILSDKFQEANKKHEIVSTEYKDLVETPDQYDGVKAY
                     PLKDELTEVVSMWEELIEEGSLICPELVKMERSGADLMRSIAIDADAPMMAPSQYDFG
                     LRCYSQAEIYTEDSRFLDAIVSYKLASAAFENSAAAAEVNKLKENIEKNGYSSYESIQ
                     MFLTMAEEKCKEAEDLWLKGNIDDIAASTALLKEAREYYSEVYSKGSNFKALEAKDMA
                     IAAQEEAIKVNADINAPDEYQNALDIFAEAEKSMEEAEYVIAYSSYVDAATAFEAARD
                     ISQAMVFDAAEAIAEAETKLADARDRASELGLEENIYLIEANLHLDNAKTLEKELKYS
                     AAIFETNEVSNYIGLSDSFVQQEAEIREKARLEQLERDKAAAEAAIADAKVRMTWAEE
                     VDLKSDYPKQYTDASNAMIAAEKAYQIEKYSSAKTLAEEVSATLSDDFQKQVLAAREL
                     EKIKAAEAEEASRLKAVAKQAADAAIADAESRMAWANENQIRAEYAQEYQNASVAMVS
                     AYVAYGNEDYLLSKQKAEEVSVILSNDFQAQVAAERAAKAQLAADKAIADEVMPKAQD
                     R"
     assembly_gap    1704..1803
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1804..2915
                     /locus_tag="PK105_07215"
     CDS             <1804..2915
                     /locus_tag="PK105_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61541.1"
                     /translation="RLAAEAKAKEEAEKEAQAMAIEIQKASNAAEALGIAHSRMSWAV
                     QANLASVYPDEFRDADTSMQAADKAYSEKAYDASIALANDVVRIISDDLMAEVNALRE
                     AEAQLAADKAIADEVMPKAQDRIEWADLNNIKADYPEVYNSAHSAMEAAQKAYQIEKY
                     AAATQLANEVLSTLSSEFEAKVTADRAEKARLAAEAKAKEEAEKEAQALIAQNKKYAE
                     VAISDARSRYDWAASKNAANNYPDLFKEGGNLLADAQTAFNALDYVRAKDLAAQAYWT
                     LMEIGEFAPLPATYKVRLIPERRDCLWRIAEYQFVYNNPYKWPVLYEANKKTFKDPSN
                     PNLIFPDQVLTIPSIKGEVRQGAWDPKKTYQPLPK"
     gene            complement(3029..4354)
                     /locus_tag="PK105_07220"
     CDS             complement(3029..4354)
                     /locus_tag="PK105_07220"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOU61542.1"
                     /translation="MEEAIKNLMDFVDASPTPFQAIDNLIGILEAHGAIKLDERELWQ
                     LEPGVSYYIVRGGSSLVTFRMGLKSPSECGFLLAGAHTDSPGLKIRPEKELQSRGYLR
                     LALESYGSPILSGWLDRPLGLAGALAIQENQEIHQRLYISHEPIAVIPNLAIHLNRDI
                     NKGFEYNLHQQLPALFGLTSHKQKEPEAASEFGKTKEISGAFDSEILVRISQELDVDP
                     KAIISSDLRLYDPEPTRIFDNSLINAPRLDDLAGCAALIEAFISLHAQASTQVACFFD
                     AEEIGSMTTGGAQSSYLRDILARITLASRSLGQDFYRALACSACVSIDGAQAWHPGYP
                     DKFDDFYTPLLGRGIALKSNANMNYATDFTSQQIGSRVASKADIKPQIYMARADIQPG
                     KTIGPITASRTGIPTVDVGHPLLAMHAIRETIAASDHLDMIAFLRAFYS"
     gene            complement(4317..4742)
                     /locus_tag="PK105_07225"
     CDS             complement(4317..4742)
                     /locus_tag="PK105_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU61543.1"
                     /translation="MIYLSIGQLEKMLSVPASTLRFWEQEVPFLMPSRTKSGRRCYSL
                     SEVVLIARLKHLALDRGLGLSYTQRILEYELLYQDQELRANIKQLREELFRLLLECDR
                     WKVSFKGLLSVSALENSTKENSEKELLNGRSDKESDGFR"
     gene            complement(4746..6191)
                     /gene="der"
                     /locus_tag="PK105_07230"
     CDS             complement(4746..6191)
                     /gene="der"
                     /locus_tag="PK105_07230"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOU61544.1"
                     /translation="MSKKKRALESLDTDSRQKAFPSQSKDGKESSPNIRYENLPLVVL
                     AGRPNVGKSTLFNRLLGKRKAITDAQPGVTRDPIEEECELLRTGEKICLVDTGGFKLE
                     REGLDELVVARALAYIQRADLVIFIIDVTQITPEDEEFAVFLRPYAKKLLLVVNKADS
                     PERDALVWEHARWGFGPMLFISAEHNRNIDKLEEAITARLDFSSVRQAEVEAADIRIA
                     IMGKPNTGKSTLLNALVGYERSIVSPEPGTTRDIVKDHFQYEGRGFEVLDTAGIRRKK
                     KVTDNIEYYSVTRSIRIVSQADVVVLLIDAEEGLSDQDKKIAAFAVEAGRPVVFALSK
                     WDKMPNMKNAFEATRDRLRFFFGQMAYAPIVPISAKERKGLDKLMSVVISLYSKANKV
                     IETSHLNQAVAEWVKETPPPASPSNRFKLRYAVQSSVNPQRFIFFVTRPEAVPESYKS
                     FLKNKIRLDFGLDGVPIQLELRASRRNRQRS"
     gene            complement(6201..>6591)
                     /locus_tag="PK105_07235"
     CDS             complement(6201..>6591)
                     /locus_tag="PK105_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOU61545.1"
                     /translation="SRSCDFFSIGTNDLSQYTLAADRGNQKVAYLNNPFDIAVLRLIS
                     MTIESGKNANIGVSLCGEMAADPASAVLLVGLGLRSLSMSASAIPAVKRALMSITMEE
                     ASSLARQALSMNTASQVSALLNSRLNL"
BASE COUNT         1798 a         1528 c         1554 g         1611 t
ORIGIN      
        1 ggcaattaaa ttcggcctag actcaatact ttccgacaaa ttccaagaag caaataaaaa
       61 acatgagatc gtatctacag aatacaagga cctcgtcgaa acaccggatc aatacgatgg
      121 agtaaaggca tatccgctga aggatgaact taccgaagtc gtttcaatgt gggaagaact
      181 aattgaggaa ggcagtctga tatgtccgga attggttaaa atggagagaa gcggggcaga
      241 tctgatgcgc tcaatagcta tagatgccga tgcgccgatg atggccccat cccagtatga
      301 tttcggtctg cggtgttatt ctcaagccga gatatatacc gaggactctc gtttcttgga
      361 tgccattgtt tcctacaaat tagccagtgc cgcattcgag aattcggccg cagcggcgga
      421 agtaaataaa cttaaggaaa acatcgagaa aaatggttat tcgagttatg agagcatcca
      481 gatgttctta accatggccg aggaaaaatg caaagaagca gaggacctat ggctaaaagg
      541 caatatcgac gatattgcgg catctaccgc actcctaaaa gaagccagag aatattattc
      601 cgaagtgtat tccaagggtt caaatttcaa agcgcttgaa gcaaaggaca tggctattgc
      661 agctcaagag gaagccatca aggtaaacgc cgacataaat gctcctgacg aatatcaaaa
      721 cgcccttgat atatttgctg aagcagaaaa aagcatggaa gaggccgagt acgtcattgc
      781 ctattctagc tatgtcgatg cagccacagc atttgaagcc gcacgagaca tttcgcaagc
      841 catggtcttt gatgccgccg aggctatcgc agaagccgag acgaaacttg cggatgcaag
      901 agatagggct tctgagcttg ggctggaaga aaacatttat ctgatagaag ccaatttgca
      961 tctcgataat gcaaaaactc ttgagaagga attgaaatac agcgctgcca tattcgagac
     1021 aaatgaggta tcgaactata taggtctgtc agattccttc gtacagcagg aagccgagat
     1081 acgcgagaag gcgcgcttgg agcaacttga aagagacaaa gctgcggcag aagcggcaat
     1141 tgcggatgca aaagtccgga tgacatgggc agaggaagtc gacctcaaat ccgattatcc
     1201 aaagcaatac acggatgctt ctaatgccat gatagccgca gaaaaggcct atcaaataga
     1261 gaaatatagt tctgcaaaga ctctcgccga agaggtttcc gcaacgctat ccgatgattt
     1321 tcagaagcag gttctcgctg ccagagagct agagaagata aaagctgcgg aagccgaaga
     1381 agcaagccga ttgaaggctg tcgcaaaaca agccgcagat gcagcaattg cagatgccga
     1441 aagcagaatg gcatgggcta atgagaatca gattagggcc gaatatgccc aagagtatca
     1501 gaacgccagc gttgcaatgg ttagcgccta tgtagcttat gggaacgaag attacctgct
     1561 ctcaaaacag aaagcagaag aagtctctgt cattctctcc aatgactttc aagctcaagt
     1621 agcagcggag cgagctgcaa aggcacagct tgctgctgac aaagcgatag ccgacgaggt
     1681 catgccaaag gcccaggatc gcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnncgcggct tgctgctgaa gcaaaggcca aagaggaagc tgaaaaggaa gcccaggcga
     1861 tggcaatcga gatccaaaag gcttcgaatg cggccgaggc tttgggtata gcccacagta
     1921 gaatgtcatg ggcggttcag gccaatctcg cttctgttta tccagatgaa ttccgcgatg
     1981 cagacacttc catgcaagcg gcagacaaag cctatagtga gaaagcttat gacgcctcca
     2041 tagcgcttgc caatgatgtt gtccgtatca tttccgatga tctgatggct gaggtgaacg
     2101 cgctgagaga agccgaagca caacttgctg ctgacaaagc gatagccgac gaggtcatgc
     2161 caaaggccca ggatcgcata gaatgggccg atctaaacaa catcaaagcc gactatcctg
     2221 aagtatacaa ttccgcgcat tctgcgatgg aagccgcaca aaaagcctat caaatagaaa
     2281 aatatgccgc cgctactcag ctagccaatg aggtgttatc aacgctctct tccgaatttg
     2341 aggccaaggt aaccgctgat cgtgccgaaa aggcgcggct tgctgctgaa gcaaaggcca
     2401 aagaggaagc tgaaaaggaa gcccaggcac ttatagctca gaataagaaa tatgcggaag
     2461 tagccatctc tgatgcaaga agccgctatg attgggcggc ctctaagaat gccgctaaca
     2521 actacccgga tctcttcaaa gagggtggaa atctgctggc cgatgctcaa accgcattca
     2581 atgctctcga ttatgtgcgt gcaaaggatc tcgctgcaca ggcttattgg actcttatgg
     2641 aaattggcga atttgctcct ctgccagcaa catataaggt aaggctcatc ccagaacgca
     2701 gagactgcct ctggagaatt gccgagtatc aattcgtcta taataatcca tacaagtggc
     2761 cggtactgta tgaggcgaat aaaaagacct tcaaagatcc ttctaatcca aatctcatat
     2821 tccccgatca ggtgcttaca atcccgagca taaagggaga agtgcgccag ggggcatggg
     2881 atccgaaaaa gacctaccaa ccacttccta agtaagagct taggctaaag aactaggccg
     2941 cccgaaaggg cggccttttt tcatcttttg ggagggcctt tttatgctta aaaatctatg
     3001 gaatctatgg aatctatgga gccgcgagct atgaatagaa tgcccgaaga aaagcaatca
     3061 tgtctaagtg gtccgaggca gcaatggtct ctcttatggc atgcatggcc aatagaggat
     3121 ggcctacgtc cactgtaggg atgcctgtcc tgcttgccgt gatgggacct atcgtctttc
     3181 cgggttgaat atcggcacgg gccatataga tttggggttt tatatctgct ttgctagcta
     3241 ctctactacc aatttgctga gaagtaaaat cggttgcata attcatgttt gcattggatt
     3301 ttaatgcaat acccctgcct aaaagcggcg tataaaagtc gtcgaattta tctggatacc
     3361 caggatgcca tgcttgcgct ccatctatag atacgcaagc tgaacaagct agcgctctat
     3421 aaaaatcctg gccaagacta cgcgaggcaa gcgtaattct ggcaagaata tcgcgcaaat
     3481 atgaggattg agctcctccg gtggtcattg atcctatttc ttcagcgtca aaaaaacatg
     3541 cgacttgtgt cgaagcctga gcatgcaaac tgataaatgc ttctatcaat gcagcacagc
     3601 cagcaagatc atccaatctt ggcgcattga tgagcgaatt atcgaaaatg cgagtaggtt
     3661 cagggtcata gagcctcaga tcggatgaga ttattgcctt agggtctaca tcgagctctt
     3721 gagaaatgcg caccaaaatt tcggaatcaa aagctccaga gatttctttt gtttttccga
     3781 attcactggc tgcttcgggc tctttttgct tgtggctcgt aagcccaaag agagcaggta
     3841 actgctgatg caggttgtat tcaaaaccct tgttgatatc gcgattcaaa tggatagcaa
     3901 gatttgggat gactgctata ggctcatgac tgatatataa acgctggtgt atctcttgat
     3961 tctcttgtat tgctagagca ccagctagtc caagcggtct atcgagccaa cctgacagaa
     4021 ttggtgatcc ataggattcg agcgcaagcc ttagatagcc gcgagattgg agttcctttt
     4081 ccggacgaat cttgaggcca gggctgtcgg tatgagctcc agcgagaaga aagccgcatt
     4141 cagaaggtga tttcagaccc attctgaagg tgactagaga gctacctcct cgaactatat
     4201 agtaggaaac acctggctct agctgccata gttccctctc atccagtttg atagctccat
     4261 gcgcttcaag aatacctata aggttatcta tggcttgaaa aggcgtgggc gatgcatcaa
     4321 cgaaatccat cagattcttt atcgcttctt ccattcaata actccttttc cgaattctcc
     4381 tttgtcgaat tctctaaagc agaaacagac agcagccctt tgaatgagac tttccatcga
     4441 tcgcattcca acaaaaggcg aaacaactct tctcttaact gcttgatgtt tgctctcagc
     4501 tcttgatcct gatagagaag ctcgtactct agtattctct gtgtgtaact aagcccaagt
     4561 cccctatcca gcgccagatg cttgagcctt gcaatgagca ctacttcgga gagcgagtag
     4621 catctgcggc cggattttgt tctactgggc atcagaaaag gcacttcttg ctcccagaat
     4681 cgcaaagtgg atgctggtac cgacaacatc ttttccaact gtccgatgct caggtatatc
     4741 atcgatcagc tccgctgtct gttcctgcgt gaagcacgaa gctccagctg aattggaaca
     4801 ccatcaagac caaaatcgag ccttatcttg tttttgagaa aactcttata ggattccggt
     4861 accgcttctg gccgagttac aaaaaagata aaacgctgcg gatttacgga actctgcacc
     4921 gcgtagcgca atttgaaacg attgcttggg cttgctggag gtggtgtttc cttgacccat
     4981 tccgcgaccg cttggttgag atgcgaagtc tcgatcacct tgtttgcctt agaatataag
     5041 gagatcacca ccgacatgag tttgtcgagc cccttccttt ctttagcgga aatcggcacg
     5101 atgggcgcat atgccatttg tccgaaaaaa aatcgcaagc ggtcgcgggt agcttcgaac
     5161 gcattcttca tgttcggcat cttatcccat ttgctcagcg caaacaccac cggtcggcct
     5221 gcttctacag caaaggctgc aatcttttta tcctggtcgg aaagcccttc ttccgcatct
     5281 atgagtagca cgacgacatc tgcttggctc acgatacgta tggagcgagt aaccgaataa
     5341 tattctatgt tgtctgtgac tttcttcttt cttcgaattc ctgcggtatc gagcacctca
     5401 aagcccctgc cctcatattg aaagtggtct ttcacaatgt cacgggttgt gcctggctca
     5461 ggagagacta tgctccgctc atagccgacc agcgcattga gcaatgtcga ttttccggta
     5521 tttggcttac ccattatggc tatgcgtatg tcggctgctt ctacttccgc ttgccgaacg
     5581 ctcgagaaat ccagacgcgc cgttatggct tcttcaagtt tgtctatgtt tctattgtgt
     5641 tctgccgaga taaacaacat cggcccgaaa ccccagcggg catgctccca taccagggca
     5701 tcgcgctcgg gactatcggc tttgtttacg acaagcaaga gctttttggc atagggcctt
     5761 agaaatacgg caaattcttc atcttccggt gtgatctgcg taacatctat aatgaagatc
     5821 actagatccg ctctctgaat ataggcgaga gctctggcca ctacaagctc atccaagccc
     5881 tcacgctcta gcttgaaacc tccggtgtcg actaaacata tcttctcccc ggttctcaac
     5941 agctcgcatt cctcttcgat aggatctcgt gtaactccag gctgtgcatc ggttattgcc
     6001 ttgcgtttgc cgagtaatct attgaatagc gtggatttac cgacattggg cctgcctgca
     6061 agaacaacaa gaggtaggtt ctcgtagcgg atatttggag aagactcttt tccatccttt
     6121 gattgcgatg ggaaggcttt ttgccgagag tctgtatcga gcgactctaa ggcccgtttc
     6181 tttttgctca taggagctaa ctacagattg agtctggaat tcagcaaggc actcacttgc
     6241 gatgctgtat tcatagataa ggcttgtcgg gccagggaag aggcttcttc cattgtaatg
     6301 gacatcagcg ctcttttgac tgcagggata gctgatgctg acatggatag acttcttaag
     6361 cccaatccta ctaggagcac tgcgctagct ggatcagctg ccatctcgcc acagaggctc
     6421 acaccaatat ttgcattctt accagattct atcgtcatgc tgataaggcg caatactgct
     6481 atatcgaatg gattattcag ataagctact ttttgattac ccctatcggc agccaatgtg
     6541 tattgactta gatcgtttgt tcctatcgag aagaaatcac aagatcttga g
//
LOCUS       DAOOWY010000100         6566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_11819_length_6566_cov_3.858547, whole genome shotgun sequence.
ACCESSION   DAOOWY010000100 DAOOWY010000000
VERSION     DAOOWY010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11819_length_6566_cov_3.858547"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1840)
                     /gene="citF"
                     /locus_tag="PK105_07240"
     CDS             complement(260..1840)
                     /gene="citF"
                     /locus_tag="PK105_07240"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOU61546.1"
                     /translation="MQNDIQWINNALGRKIPLNIDGRLNKPFQDVFSDATQEQRGRKI
                     KERAVQPSAISQRQKPDKLVASWDELFERLDIQDGATISFHHHLRNGDKIVNEVVARL
                     ASRGIMGLTLAPSALFSVHDALLPYIKDGVIAKIEGSMNGAIGAACSHGEIAQTCILR
                     SHGGRQRAIREGDLPIDVAFIAAPCADSFGNANGLYGSSACGPLSYAKPDSEYAKKVV
                     ILTDNLVPYPCSSWSIRGGNVDYVLNVESIGDSSRIVSGTTQLTKSPTQLVIARLAAR
                     FIEAAGLMKDGFSFQAGAGGISLAATVFLAERMRNAGIKASFAHGGATKILVDLLEEG
                     LLDSIIDLQSFDLDAVRSCAKNEKHIESDPFSSYGPHANCCVANMLDIALLGATEIDL
                     NFNVNVVTHSDGILLHGIGGHSDASSGAGCTIITAPSFRKRIPIIKEEVTTITCPGEL
                     IDVLITERGIAINSRRDDLLWSAKKADLPCVDISQLMHDVYQITGVPEKPHFTDHPVA
                     LVEWRDGSLIDTIWQIEE"
     gene            complement(1830..3167)
                     /locus_tag="PK105_07245"
     CDS             complement(1830..3167)
                     /locus_tag="PK105_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOU61547.1"
                     /translation="MSIRDQFEAGFVSKSDCLVKYQATAEPLSIEVHSSVQSLFGNAI
                     RTCAMQMLEEFKVTTGALLVDDDGALDLVIRARIEAVLRSAGYLSQKRLFVDASSGIS
                     WQTGVSTIESEKNASRAENSASLDGGIERPVCSLVKQQARLYMPGDQPHFAINADFYG
                     ADILIFDLEDSVPLARKSEARILVRRFLENSFLFRKSERAVRINPLSTEESVHDIAEI
                     VPAMPDIILLPKCESAMDVERLDTLLTANEEEYGLVPGSIRIMPIIESAKGVLAAYEI
                     ARASQRNSALCFGYEDFSRDIQSKPDIVKVAHVQDDGSQKVNIPSNPVSSIDSLLARQ
                     IIILASRAADIEPLDSSYSDIGNTEGLLNSCYEARALGFTGKSVIHPTQIPYVKKAFL
                     PSDEEVQHAKAVIAAYEESLKAGKGAVAIDGQMIDIPVVEKARRIIEESMHAE"
     gene            complement(3164..5422)
                     /locus_tag="PK105_07250"
     CDS             complement(3164..5422)
                     /locus_tag="PK105_07250"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU61548.1"
                     /translation="MANHIPEDYIERVYAGLIGKNIGIRLGAPVEPAIWTYERIRDVY
                     GEVSSYLKPYRNFAADDDANGPVYFVRPLLEKVEEALKALKLKALKDEELKKNQRNET
                     QNTANNEKGLNQIQIQPSDIAKAWLNYAREGAGMFWWGGYGRSTEHTAYLNLKNGIPA
                     PESGSARRNSKVVAEQIGGQIFIDTWGLVWPGNPQKAAQFAESAASVSHDGEGLNGAR
                     FIAGCIAAAFCEPDISIVMDIGLSLMPPNSAYAAVIQAVKQFYKTNPDNWRLCQEYLI
                     AEWGYDKWPGLCHIIPNAGVCALALLYGRGSFSRTVEIATMCGWDTDCNAGNVGTIAG
                     VLYGLDALPDHYRKPINDSIVLSGLPGALNVLDLPTFARRLAAVGYVLAGEDVPKTIA
                     ESLERGDGEQAIYFDFRLSGSTHGMRVSDPVRFGTCHRTLEYKGAQQGALEFRFERLI
                     REESGTCFFKPFYRRYDFDDERYSPVFSPIARPGQHVEITFCVECWEGQRIGIEGYVR
                     DTWSRKEIITTPTIFIEASKEQASESMEEYNLSFDIPGMHGSSADEIGLKITSFSGKG
                     KRDSGRLFIHRFAIEGKACYEIDLSKQAIEFGSITPFSHEGGTWSIEGSRLQLMTPAK
                     AASYTGGYRVGDQKISTTITPQAGDCHMLVVRAKGAQQAYWGGLDGKGKIALYRQNFG
                     FEKMAEADFDWAYDMYIEIALEAIGKDISLWIDGQKMLEAHDEALKSGMVGCGTLRAS
                     RTLFGPFIVEEL"
     gene            complement(5675..>6566)
                     /locus_tag="PK105_07255"
     CDS             complement(5675..>6566)
                     /locus_tag="PK105_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61549.1"
                     /translation="QKKGAQYGISVTGATNGAEELDSIFDAAFGITSTWMKGSDGHWV
                     YCRVTPNEKEKLEFYVKLYKEGLLDPEYLTKKWDTKEQAFYEGKAGIVPGVAPSSIDV
                     YANKMMKTQNTTIVPLPPPAGKAQGLGPFVDVIKESRGFAIPVTSKVKDSAFAILDFI
                     WSPAGLKIAKLGIPGVHYIEEPNRYVITDRYPEWVNSWFGDSFNGFNPDKPLSRPIIT
                     DAAVESGKLAIKYMMPDKTFLIPEQYITNWDAMTNLYKEYEADIITGKKPISAFDEFV
                     QKWYRAGGTDLTKYAQTVLK"
BASE COUNT         1679 a         1618 c         1387 g         1882 t
ORIGIN      
        1 atgttgttct tggtcccata attggtggtg atggttgcgt ctggatctaa gcgaacaaga
       61 ctggacacaa tcatgactag gccaaagtat ccatgcgctg atgatctaag ccctttgatc
      121 tggccatatg taacatcatt tacctgctcg aaattacgaa cttgacttta cactatcatt
      181 gaaagaggct ctatattgaa gtctgttctt ttaatttttc agcaggtcgt ttaagtaggg
      241 gaatctagct cattactctc tattcttcta tttgccatat agtatcgata agactgccat
      301 cgcgccattc aacaagggca acagggtggt cagtgaaatg aggtttttct gggacaccgg
      361 tgatctgata cacatcgtgc atgagctgcg agatgtccac gcagggtaag tccgctttct
      421 tggcactcca tagaaggtcg tcgcgtctgg aatttatggc tattccgcgc tcggtgatga
      481 gcacatcgat gagctcacca gggcaggtga ttgtagttac ttcttctttg atgatgggga
      541 ttctcttgcg gaaggatggt gcagtgataa ttgtacaccc tgcgccgctg ctggcgtcgc
      601 tatgaccacc aataccatgc aggagtattc catcagaatg ggtgacgaca tttacgttga
      661 aattgagatc gatctcagtt gcaccaagca gtgcaatatc cagcatgttt gctacgcagc
      721 agttggcatg aggaccatat gagctaaatg gatctgattc tatatgcttt tcattttttg
      781 cgcaggagcg cactgcatcc agatcaaagc tttgaagatc aatgatcgag tctaaaagcc
      841 cttcttctag aagatcgact agaatttttg ttgcgcctcc atgagcaaat gacgccttta
      901 tccccgcatt gcgcatgcgt tcagcaagaa aaacggttgc tgcgagcgat atgcctcctg
      961 cacctgcttg aaatgaaaaa ccatctttca taaggcctgc tgcctcaata aatcgcgcag
     1021 caaggcgtgc aatgaccaat tgcgtagggc tttttgtgag ttgtgtagta cctgagacta
     1081 tccgtgaact gtcgccaata ctctcaacgt ttaagacata atcgacattt ccacctcgaa
     1141 ttgaccaaga gctgcaagga tatggcacta gattgtcggt taaaatcaca acctttttgg
     1201 catattctga atcgggtttt gcatagctga gcggtccaca ggctgaggaa ccataaaggc
     1261 catttgcatt gccaaatgaa tcggcgcaag gtgcagcgat aaacgcgaca tcgattggca
     1321 ggtcgccttc tcgaattgct ctttgacggc ctccatgaga acgcaatata catgtttggg
     1381 ctatttcacc atgggagcaa gccgctccaa ttgcgccatt cattgagcct tcgattttag
     1441 caatgactcc atcttttatg tacggcaaga gggcatcgtg aacagaaaag agcgctgaag
     1501 gagctaaagt caatcccatg atacctcgcg atgcaagccg tgcaactact tcattgacta
     1561 ttttatcgcc atttctcaag tggtgatgaa acgagattgt tgcaccatct tgaatatcga
     1621 gtcgttcaaa gagttcatcc cacgatgcta caagcttgtc tggtttttga cgctgtgaaa
     1681 ttgccgacgg ctgtactgct cgctctttga tttttctccc tctttgttcc tgtgtagcat
     1741 ccgaaaatac gtcttgaaat ggcttattca aacggccatc tatattcaat ggtatttttc
     1801 taccgagcgc attgtttatc cattggatgt cattctgcat gcatgctctc ctcaatgatg
     1861 cgccttgctt tttcgaccac aggaatatcg atcatctgtc catcgatagc gactgctcct
     1921 ttgccagctt tgagcgactc ttcgtaagca gcgatgacag ctttcgcatg ctgtacttct
     1981 tcgtctgaag gcaagaatgc cttttttaca tatggtatct gcgttggatg gataactgat
     2041 ttccctgtaa aaccaagtgc acgcgcttcg tagcaggaat tgagtaatcc ctcggtattt
     2101 cctatatcgc tatatgagga atcgagcggc tctatgtccg cagcgcgcga cgcaaggatg
     2161 attatctgcc gcgcaagcag cgaatcgatg gagctgacag gatttgatgg aatattcacc
     2221 ttctgtgagc catcatcctg aacgtgcgca acttttacta tatcaggctt tgattgaata
     2281 tccctcgaaa aatcttcata gccaaaacag agtgcgctat tccgctgcga ggcacgcgca
     2341 atttcatatg ctgcaagcac tcctttggca ctctcaataa ttggcatgat acgaatagag
     2401 cctggaacga gaccatattc ttcttcattg gctgtgagaa gtgtatcaag tcgctctacg
     2461 tccatggcgg actcgcattt tggcaagaga ataatgtccg gcattgcagg cacaatttca
     2521 gcaatatcat gcaccgattc ttcagtagag agcggattga tcctcaccgc gcgctcgctt
     2581 ttcctaaaca agaatgaatt ttctaaaaat cgacgcacaa gaattcgagc ttcggatttt
     2641 cgggctaaag ggaccgagtc ttcgagatcg aatatcagga tatcagcacc atagaaatca
     2701 gcattgatgg cgaaatgggg ctgatcgcct ggcatataga gtcgtgcttg ctgctttaca
     2761 aggctgcaaa ctggccgctc gatgccgcca tccagactcg cgctgttctc agctcgactc
     2821 gcgtttttct cactttcaat agtcgatacg cccgtttgcc acgatatccc agacgatgca
     2881 tcaacaaaca atcgtttttg cgataaatac cctgcgctcc gtagcaccgc ctcaattctc
     2941 gcgcgaatta caagatcgag cgccccatca tcatcaacca aaagcgctcc tgttgtcact
     3001 ttaaactctt cgagcatttg catggcacac gtacgaatcg catttccaaa aagcgactgc
     3061 acacttgaat gtacttctat cgatagcggt tccgctgtag cttgatattt tactaaacaa
     3121 tccgattttg aaacaaaacc agcttcaaat tggtcccgaa tgctcataat tcttctacta
     3181 taaaggggcc aaagagcgtt cgcgaagctc gcagcgtacc acatccaacc attccgctct
     3241 tcagagcctc atcatgtgct tcgagcattt tttgcccatc gatccacaaa gaaatatctt
     3301 tccctatcgc ttcgagcgct atttctatat acatatcata cgcccaatca aaatccgcct
     3361 ctgccatttt ctcaaaccca aaattttggc ggtagagagc tatctttcct ttgccatcga
     3421 gcccgcccca gtatgcctgc tgggcaccct ttgctctgac tacgagcata tggcaatccc
     3481 ccgcctgggg agttatcgtt gtacttattt tttggtcgcc gactctatat cctccggtat
     3541 atgatgcagc ttttgcaggc gtcatgagct gaagcctgct gccttcaatc gaccaggtac
     3601 caccttcgtg agaaaatggt gttatcgatc caaattcgat tgcctgcttt gaaagatcga
     3661 tttcatagca tgcttttcct tcgatggcaa aacgatgaat aaaaagtctg ccagaatcgc
     3721 gttttccttt acctgagaag cttgtgatct taaggccaat ttcatcggcg gatgatccgt
     3781 gcataccagg aatatcaaat gataaattat attcctccat cgattcggat gcctgctctt
     3841 ttgaggcttc aataaaaatt gtgggcgttg tgataatttc tttacgagac cacgtgtcgc
     3901 gcacataacc ttctattcca atgcgctgac cttcccagca ttcaacgcaa aacgtaattt
     3961 cgacatgctg cccaggacga gcaattggcg agaatacagg gctatagcgc tcatcatcaa
     4021 aatcatacct tcgatagaag ggtttaaaaa aacaagttcc actctcctcg cgaatgagtc
     4081 gctcaaatcg aaattccagt gcgccttgct gcgctccttt atattcgaga gtacggtggc
     4141 aggtcccaaa gcgaacagga tctgagactc tcatgccgtg cgtcgatcca gagagacgaa
     4201 aatcgaagta tatcgcctgt tctccatcac ctctttcgag gctctctgca atagtctttg
     4261 gaacatcctc cccagcaagg acatagccaa ctgcagcaag ccttcttgca aacgtgggca
     4321 gatcgagcac attgagcgca ccaggcaatc ctgaaagtac gatgctgtcg ttgatgggtt
     4381 ttcgataatg atctggcaaa gcgtcgagcc cataaaggac tccagcaata gtacctacat
     4441 tgcctgcatt gcagtctgta tcccagccac acatcgttgc aatctcaaca gtacgcgaga
     4501 aactccctcg accatagagc aatgcaagtg cgcatactcc tgcattcggt attatatggc
     4561 aaagccccgg ccatttgtcg tatccccatt cggcaatgag atactcctgg cataggcgcc
     4621 aattatctgg atttgtttta taaaactgtt tgactgcctg tatcacggca gcataggctg
     4681 aatttggtgg catgaggcta agaccaatat ccatcacaat agatatatct ggttcgcaaa
     4741 atgctgcggc gatacagcct gcaataaagc gagcaccatt gaggccttct ccatcatgcg
     4801 aaacagatgc tgcactctca gcgaattgag cggctttttg aggattccct ggccatacga
     4861 gcccccaggt atcaataaaa atttgaccac cgatctgttc agcaaccact ttgctatttc
     4921 tgcgcgccga cccagattct ggcgcaggaa taccattttt aagattgaga tacgcagtat
     4981 gctctgtgct tctgccgtaa ccaccccacc aaaacatgcc agcgccctca cgggcatagt
     5041 taagccaggc tttagctatg tccgatggct gaatctgtat ttgattgagc cctttctcat
     5101 tattcgcagt attttgtgtc tcatttcttt gattcttctt aagttcctca tctttgagag
     5161 ccttcagctt gagagccttc agcgcctctt cgaccttttc aagcagcgga cgaacaaaat
     5221 atactggtcc atttgcatcg tcgtccgctg caaaattccg atatggctta aggtaagagg
     5281 agacttctcc atagacatcg cggatgcgct cataagtcca gattgctggc tcaactggtg
     5341 cgccgagccg aatgccgata tttttcccta ttagccctgc atagacgcgt tctatatagt
     5401 cttctggaat atgattggcc atagattgtt ctttttctcc taaaattatt ggcacaaggg
     5461 aaattttgca aaatctataa aatattcaat cattgcgcgt ctagattgcg catctagatc
     5521 gcgcatgcgc tttcaaattg cccctgtgct tctttcgcta ttactcgatt actcgtgcct
     5581 ctttaactat tacttgcgcc tttatcgcta ttacttcaaa tctgtacttc aatatcgata
     5641 ggctctttcg atggtttaaa tagaaaattt agctctattt caacacagtc tgcgcatact
     5701 ttgtcaaatc cgtacctcct gctcgatacc atttctgaac aaactcatca aatgctgaaa
     5761 taggcttttt gccagttatg atgtctgctt cgtattcttt atagagatta gtcattgcgt
     5821 cccaattcgt tatatattgt tcaggaataa gaaatgtctt atcaggcatc atatatttga
     5881 ttgcgagctt tccagattct accgcagcat cagttataat cggacgcgag agcggcttgt
     5941 cgggattaaa accattaaat gaatcgccaa accaactatt gacccattcg ggatatctgt
     6001 cagtaattac ataacgattt ggctcttcga tataatgcac accggggata ccaagcttcg
     6061 caattttaag gccagcaggg ctccagatga agtctaaaat agcaaaggcc gaatctttca
     6121 cctttgaagt gacagggatt gcaaatcctc gagactcctt aatcacatcg acgaatggcc
     6181 caaggccttg tgccttgcct gcaggaggag gaagcggaac aattgttgta ttttgagtct
     6241 tcatcatttt atttgcatac acatcaatac tcgatggtgc gactccaggc acaatccctg
     6301 cttttccctc ataaaaggct tgctcttttg tatcccattt ttttgtcaga tattcaggat
     6361 caagtaagcc ttctttgtag agctttacgt agaattcaag cttttctttt tcatttggtg
     6421 tcaccctgca atatacccag tggccatcgc tgcctttcat ccaggtagat gtgatcccaa
     6481 aagccgcatc aaaaatagaa tcgagttctt ctgcaccatt tgtggctcct gttacagaga
     6541 tgccatactg tgcacctttc ttctgt
//
LOCUS       DAOOWY010000101         6514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_11928_length_6514_cov_11.942096, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000101 DAOOWY010000000
VERSION     DAOOWY010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11928_length_6514_cov_11.942096"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1171
                     /locus_tag="PK105_07260"
     CDS             245..1171
                     /locus_tag="PK105_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61550.1"
                     /translation="MDLIRFITRRFLRLILTAFIIATIVFFVIRVIPGDPALVIAGID
                     ANPADVQAIRVRLGTDRPLLVQYGDFLWHIARLDFGNSMISGEPVLKLILERFPLTLK
                     LAIFGIAFSIVIAVPLGVLSAVKRWSAWDYFGLVLSQLGMAIPSFWFGIILLLIFAIK
                     IPIFPLFGSETFAHYILPIVSLGFGRAAIILRHTRASMVEELSKEYIITARAKGLTER
                     MVKYKHALKNALLPIITVTSIELGYMLGGAIIIEQVFSMPGLGRLFLSGIYQRDFPII
                     QGGVVFVAILFSSINFIADILYSIANPKIRLN"
     gene            1172..2155
                     /locus_tag="PK105_07265"
     CDS             1172..2155
                     /locus_tag="PK105_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61551.1"
                     /translation="MDRMVSLVSIEDLRVAFSFGNKEATVLRGMNLSVLKNEIHGLVG
                     ESGSGKTVTSTCIMRLLPIPPAKILSGHIYFDGKDLLSLSGEQMRTYRGRRIAMIFQE
                     PAKYLNPAFKIGEQIIEMLMLHMKMHKQSACKRAIELLKLVGLDEKVIDKYPHELSGG
                     MKQRAMIAMAISCDPDFLIADEPTTALDVTLQIQILALLKKLKDMKKLGVLFISHDLH
                     LVSEIADRVSVIYAGRIVESAVKEDLFKNPLHPYTRLLMHSIPDAKKKGSRLATIPGQ
                     VPDAAEIGNGCAFANRCPIAKEGCFADIPGYVEYENEHTVACYYAGKIWEI"
     gene            2143..3129
                     /locus_tag="PK105_07270"
     CDS             2143..3129
                     /locus_tag="PK105_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU61552.1"
                     /translation="MGNLIETKDITKIYRSRGLGGSKHGFKAVDCVSLTIRKQSIFGL
                     VGESGCGKTTFGRALMYLDPPTSGSVVFDDVSLGSLKPKELRRFRNRMQIVFQDPNSS
                     LDPKMSIYDSLSEGMVNLKIPREQRDRKVNNLINLVGISKTYLHRFPHELSGGQKQRI
                     GIARALSVDPDFLVLDEPTSNLDVSIQAQIINLLLDLKNEFGLTYLFISHDLNLVSYM
                     CDEIAVMFKGRIVEKAKTEQLISKPSHSYTQRLLSSIPGGPQRIGVSCLNIEDLDEKT
                     IKERQEHEKSRSACLYYSQCPMGDAECSLSAPSLRELSGGHVVSCHKIRTGK"
     gene            3153..4652
                     /locus_tag="PK105_07275"
     CDS             3153..4652
                     /locus_tag="PK105_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61553.1"
                     /translation="MKRAVIVGMVFFAIISGAGGYLFAQGKSSITFGLSGNPDTLDPQ
                     KTAGTLTFQVVKSLYDTLVEPDTTGKLVPALAESWTVSPDGLTWTFKLRQGVVFHNGQ
                     PLTSRDVKATLDRIRDPSFASPRRNEFTAIKEVRTPDPQTAVIVLGAPYAPILANLAS
                     GWGAILPADLIQSGHNFDSEPIGTGPFKFEKWIRDNKISMVRNEKYWMKGLPKLARVD
                     FQIVPERSVQIQGLMSGAIDAVEYVDPEDLPILQSNPNVTIRKDQTSLILVMAMNCSR
                     EPLNDLRVRQAINYAVDKQLVLDVAYGGGKVGSSFMDTGNAYYTDFSYLYPYNPEKAK
                     QLLKEAGIGNRQFTITVPQNYPLHVKAGELIQQMLEKVGMNVKIQLVDWSTWLARIYT
                     EGNFDLTIIGHTGKLDPDGTLAGYGTDKKYVRWINPTCDAKIKEAATISDFAARKKLY
                     TEALEIMAKEVPFVYLGSSYRYVAMRSNVVDFRITPNLDSYDFRWTELK"
     gene            4659..5483
                     /locus_tag="PK105_07280"
     CDS             4659..5483
                     /locus_tag="PK105_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61554.1"
                     /translation="MFGKSQKILFDLAVFYLLILVVSAIAAPLITPYIPTEQFLSKRF
                     HPPSFDNIMGTDNFGRDILSRIIYGSRSALIVGIVTVTIALAIGMAIGLLAGMGSKSL
                     DNVLMLFMDSLLSFPTILLAITVVSFLGYGIVQVMLALGIISSPVFARLIRAETLAIK
                     TEGYVEAAKALGTSSFKIAFRHIIPNLLGKVAVQCSLTFGQAIVIESSLSYLGVGTQP
                     PDASWGLMLKDARNYLIQAPWMAIYPGLCLALTVLAFNIIGDSLAESLNPRLTGSS"
     gene            5564..6379
                     /locus_tag="PK105_07285"
     CDS             5564..6379
                     /locus_tag="PK105_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HOU61555.1"
                     /translation="MIAGIDIGSTSTKVVVLDNDSISCRMRVRASDAVAAAAGALGKL
                     DTEQGYKIQDFDRVAITGAGASKIHKDIFDIPTIRIEEITAIGHGGKFLSKSDDIIIA
                     NIGTGTPFIEANRNRIAHIGGTGVGGGTIIGLAKALLKTSDFETIMDLASRGDLSHVD
                     LMIQDIAETNISFLAKDVTASNFAKMLENATREDIAIAIINMVYQVIGMLSVFVAQSR
                     NKKNIVVTGNGSNNIIGRNVLAGISEMYGINFEFPEDAEFSTAIGAVLAASSA"
BASE COUNT         1864 a         1266 c         1565 g         1819 t
ORIGIN      
        1 tggtcaacgg aagaagaata ttaagccgcg caccgagtca atgcacctcg aagtagaatg
       61 taatgatttc aaaatcccca tagaaaaaat tttgcttgac aacagccaga atggataata
      121 aattgttaaa tataggttga tttttgccat cagcgtaata tagaagggag catttttatt
      181 gaaatcgagc aatatctata taattaatcg tcatatcgac agatgatatc ctagtgagcc
      241 aatcatggat ttaattcgct tcattacaag aagattcttg agacttatac tgacagcatt
      301 tataattgcc acgatagttt tctttgttat tcgcgtaatt cctggagatc ctgctttggt
      361 tatcgctgga attgatgcaa atcctgctga tgttcaagca atccgggtaa ggttggggac
      421 agatcgccct cttcttgtcc aatatggaga ttttctttgg catatagcgc gccttgattt
      481 cggcaattca atgatctccg gggaacctgt tctcaaactt attctagagc gatttcctct
      541 tactcttaag cttgcaatct ttgggattgc attcagcatt gtaattgcag taccacttgg
      601 tgtcttatct gctgtaaagc gatggtcggc ttgggattac tttgggttgg ttctttcaca
      661 actaggaatg gctattccta gtttttggtt tggcataatt ctgcttttga tttttgcaat
      721 caagatacca attttcccgc tttttggttc ggagaccttt gcacattaca ttctgccaat
      781 agtttcgcta ggatttggtc gtgcagcaat tatcttacgc catacaagag catctatggt
      841 ggaagagttg tccaaggaat acataattac tgcaagggca aaaggcctta ccgagcggat
      901 ggtaaaatac aagcatgctc taaagaacgc tcttttgcca atcattaccg tgacaagtat
      961 tgagctgggc tatatgcttg gaggcgcaat cataatagag caggtttttt cgatgccggg
     1021 tttgggaagg ctcttcctgt ccggaatcta tcagcgcgat ttcccgatca tccaaggagg
     1081 tgtggttttt gtagccatcc tgttctcttc gataaacttt attgctgata ttctttacag
     1141 cattgctaat cctaagataa ggctaaacta gatggataga atggtatctc ttgtatcgat
     1201 agaagacctg agagtcgcat tttcttttgg caataaagaa gccaccgtat tgcgaggcat
     1261 gaatctatct gttttgaaga atgaaatcca tgggctcgtc ggagaatccg gatctggcaa
     1321 gacagtgact tctacctgca taatgagact tttgccgatt cctcccgcta agattctatc
     1381 gggtcacatc tattttgatg ggaaggactt gttgtcttta tcaggggaac agatgcgaac
     1441 ctacagaggc agaagaattg ccatgatatt tcaggagcct gcgaagtatc tcaatccggc
     1501 attcaagatc ggagagcaga ttattgaaat gctcatgctt catatgaaga tgcacaagca
     1561 atctgcttgt aaaagggcta tagagctatt gaagttggta ggcttggacg agaaggttat
     1621 cgataagtat ccgcatgaat tatcaggagg gatgaagcaa agagccatga ttgccatggc
     1681 tatttcgtgt gatcccgatt ttctcatagc tgatgagccc acaacggcgc tcgatgtgac
     1741 attacagatt caaattctcg cattgctgaa aaaactaaag gatatgaaaa aattgggtgt
     1801 cttattcatt tcacacgatc ttcatcttgt aagcgagatt gccgatcggg tttcggttat
     1861 ctatgctgga agaatagtcg aaagtgcagt gaaagaagat ctcttcaaga atccattgca
     1921 tccatatacg cgtttgctta tgcattccat tccggatgct aaaaagaaag gatcaagact
     1981 tgctacaatt ccggggcagg ttcccgatgc ggcagaaata ggcaatggct gtgcatttgc
     2041 aaatcgttgt ccaattgcga aagagggctg ctttgcggat attccagggt atgttgaata
     2101 tgagaatgaa catacggtcg cttgctatta tgcaggtaag atatgggaaa tctgatagaa
     2161 accaaggata taacaaagat atatcgctct cgtgggctag gtggatcgaa acatggtttt
     2221 aaggccgttg attgtgtttc cctaaccata agaaagcaga gtatttttgg gctcgtagga
     2281 gagtcgggat gtggcaaaac gacgtttgga agagcgctta tgtatcttga ccctccaaca
     2341 tccggttcgg tggttttcga tgatgtgtcg cttggatctc tgaagcctaa ggaattgaga
     2401 agatttcgaa atagaatgca aattgttttt caggatccga acagttcgct ggatcccaag
     2461 atgagcatct atgatagtct atcagaaggc atggtgaatc taaaaattcc tagagaacaa
     2521 cgagaccgca aggtcaacaa tctcatcaac cttgtaggga tcagtaaaac atatctgcat
     2581 cggtttccgc acgaactctc tggagggcaa aagcaaagga tcggtatagc tcgcgctctt
     2641 tctgtagatc ccgattttct tgtactggat gagcccacat ccaatttaga cgtatctatt
     2701 caggcgcaga taataaacct attgctggat ttgaagaatg aatttggatt gacttatcta
     2761 ttcattagcc atgatctcaa tcttgtatcc tatatgtgcg atgaaatagc ggtcatgttc
     2821 aagggcagaa tcgtagagaa agcgaaaaca gaacaactta tttcaaaacc aagccactca
     2881 tatacgcaaa ggcttctcag ttcgataccg ggaggtccgc agagaatagg tgtatcctgt
     2941 ctgaacattg aggatctcga cgagaagaca ataaaagagc gccaagagca cgaaaaatct
     3001 cgctcagctt gtctttacta ttcgcaatgt ccaatgggcg atgctgaatg cagcttgtct
     3061 gctccttcat taagggagct ttcaggaggg cacgttgtgt cctgtcataa aatcagaact
     3121 ggcaaataac tcgtatttct aaggagaaaa agatgaaaag agcggtgatt gtgggaatgg
     3181 ttttttttgc cattatatct ggggcagggg gttacctttt cgcacagggg aaaagttcta
     3241 ttacatttgg tctatccggc aatccagata cgcttgatcc tcagaaaaca gcaggaacgc
     3301 tgacattcca agttgtaaag agcctttatg atactttggt agagcccgat acaacgggta
     3361 agcttgtgcc ggcgcttgct gagagttgga cggtttcccc agacggatta acatggacat
     3421 tcaagctaag gcaaggtgtc gtgttccata atggacagcc attaacctct cgggatgtaa
     3481 aggcgacatt ggatcgcata agagatccga gctttgcttc accgcgaaga aacgaattca
     3541 cggcaataaa agaagtgagg acaccggatc cacagacggc tgtgattgtt cttggtgcgc
     3601 cttatgcgcc gattcttgcc aaccttgctt ctggctgggg agcaatcctg cctgctgatc
     3661 ttatccaatc tggtcacaac tttgactctg aacccatcgg aacagggccc ttcaagttcg
     3721 agaagtggat tcgcgataac aagatatcga tggtcaggaa tgaaaaatac tggatgaagg
     3781 gtttacctaa attggcaagg gttgatttcc agatagttcc agaaagatcc gttcagatac
     3841 aaggattgat gtctggtgca atcgatgctg ttgaatatgt cgatccggag gacttgccaa
     3901 tactgcaatc aaatccgaat gtcacgataa gaaaggatca gacctcgttg atcttggtta
     3961 tggcaatgaa ttgctctcgt gaaccactca acgatcttcg cgtgcgccag gccataaatt
     4021 atgcagtcga taagcagctt gtattggatg tagcatatgg cggcggaaag gtcggctctt
     4081 ctttcatgga tacagggaat gcttactata cagatttttc ttacctttat ccttataatc
     4141 ccgagaaagc gaagcaattg ctgaaagaag ccggaatagg caataggcaa ttcactataa
     4201 ctgtgcccca gaactatccg ttacatgtga aagccggaga actaattcaa caaatgctcg
     4261 aaaaagttgg catgaatgtc aagatacagc tcgtggattg gtcaacctgg ctggctagaa
     4321 tctatactga aggcaatttc gatttgacaa ttattgggca taccggcaaa ctcgatccgg
     4381 atgggacgct ggccggatat ggcacagaca agaagtatgt caggtggatc aatccaacct
     4441 gcgatgcaaa aataaaggag gccgccacca tctctgattt cgctgctcgc aagaagctat
     4501 atacagaggc attggagata atggcaaaag aggtaccttt tgtatacctt ggaagttcat
     4561 atcgctatgt agccatgaga tctaatgtag tagactttag gattacccca aacctggatt
     4621 catacgattt tcgatggaca gaattgaagt agaagagtat gttcggaaag tcccagaaga
     4681 ttttgtttga ccttgctgta ttttatctcc tcatattggt tgtatcagca atagcggctc
     4741 cgctgataac accatacatt ccaacggagc agtttctctc taagcgtttc catccgccca
     4801 gctttgataa tattatgggg acggataatt ttgggcgtga tatcctgagc agaatcatat
     4861 atggatctcg gtcggcgctc attgtgggca tcgtaaccgt gacaattgcg cttgcaatag
     4921 gaatggccat agggttgctt gcaggcatgg gctcgaaaag tttggacaat gtgttgatgc
     4981 tattcatgga tagtctactt tcatttccta cgattttgct tgcgatcacc gttgtgtcgt
     5041 ttctgggata tggaattgtt caggtaatgc tcgcgttagg aataatcagt agccctgtat
     5101 ttgcaaggct cataagggcg gaaacgcttg caataaagac cgaaggatac gtggaagctg
     5161 caaaagccct gggtacatcc tcatttaaaa tagctttcag gcatatcatc ccaaatcttc
     5221 tgggaaaagt agctgttcaa tgttctctca cctttggtca ggcgattgtc attgagtctt
     5281 ctttatcgta tctgggggtt ggaacccagc ccccagatgc tagttggggc ttgatgttga
     5341 aggatgcgcg taattacctc attcaagctc catggatggc tatataccca ggtctctgtc
     5401 tggccttgac agttcttgca ttcaatatta ttggtgattc gcttgcagaa tcgcttaatc
     5461 caaggctcac tggttcttct tgagatatgg atagagagag aaggcagtgg caatttgcat
     5521 tttgtagttc atttttagtt tgccgaagga ggaaggagca aagatgatcg ctggcataga
     5581 tattggaagc acaagtacaa aagttgttgt gctcgataat gattccatct cgtgcagaat
     5641 gcgcgtgcgc gcttcggatg ctgttgccgc agcagcaggt gcattgggta agcttgacac
     5701 cgagcaagga tataagattc aagacttcga tagagttgcg ataaccggtg caggtgcatc
     5761 aaagattcat aaagatatat ttgatatacc aacaataaga atagaagaaa ttactgctat
     5821 cgggcatggc ggaaagtttc tttccaagag cgatgatatc atcattgcca atatcggaac
     5881 cgggacacca ttcatcgaag caaatcgaaa tcgaatagct catataggtg ggacaggtgt
     5941 gggcggaggc acgatcattg gcctagcaaa agcattgctg aagacaagcg attttgaaac
     6001 cataatggat cttgcttcta gaggggactt gagccacgta gacctaatga ttcaagatat
     6061 tgcggagaca aacatcagtt tcctcgcaaa agatgtgacg gcctcgaatt tcgcgaagat
     6121 gctggaaaat gcgacaagag aagacatcgc aattgctatt atcaatatgg tgtaccaggt
     6181 aatcggaatg ctttctgtat ttgtggcaca aagcagaaat aaaaagaata ttgtcgtgac
     6241 aggcaatgga agcaacaaca taattggaag aaatgttctt gcgggtatat ctgaaatgta
     6301 tgggatcaat tttgaattcc cagaagatgc agaattttct accgctattg gagcggtact
     6361 ggctgcaagt tcggcatagc caatagagat aatctatatc gacagagaaa aatgcgaatg
     6421 aagttccgct ttaaggataa aggcaaagga gtctaaaaga tgagagattc aatcacggat
     6481 gttcctggta tcagggtagg aaacgctcag gata
//
LOCUS       DAOOWY010000102         6499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_11962_length_6499_cov_8.571850, whole genome shotgun sequence.
ACCESSION   DAOOWY010000102 DAOOWY010000000
VERSION     DAOOWY010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11962_length_6499_cov_8.571850"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1722)
                     /locus_tag="PK105_07290"
     CDS             complement(7..1722)
                     /locus_tag="PK105_07290"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOU61556.1"
                     /translation="MKKETKPQYVIGIDYGTDSCRAVIIDAANGYEISSAMAAYPRWS
                     KGLYCDPGKNQFRQHPMDYIESLEIVMKQIHDKTGQDVLPFVEGIAIDTTGSTPCLIT
                     DTLCPLAMLPEYKDNPDAMFILWKDHTSTEEAFEITELARRWSVDYTMYEGGTYSSEW
                     FWAKLLHIARTDRHVMNAGFSAIEHCDWMPALLTGTNALPKIKRSRCAMGHKAMWHES
                     FGGYPSDDFFAALDMQLVRIKKTLGRDTYTSDTEFGKLSNEWAERLHLRSGIPVAVGA
                     YDAHVGAVGGGVEEKVLVKVMGTSTCDMIVGPRTSSENPETSEHLVKGICGQVDGSII
                     PGMIGYEAGQSAFGDVYAWFKDLLSWPLKEMKSESMESAKELGIPSNTLDDLADSIDS
                     RLIFAIDKAASKIQPEESSVLALDWLNGRRTPYANQKLKGAIIGLSLGTTAPMIYRAL
                     IEATAYGSRAIIERFEQENIHIEKVRAVGGVARKSKLVVQIVADVLGRPIEIIASDQS
                     VALGAAMFASVVAGIYDSVTQAQLAMKPDIETVIQPDFVRTSLYDALYRDYLKLGAFI
                     ENELT"
     gene            complement(1815..2597)
                     /locus_tag="PK105_07295"
     CDS             complement(1815..2597)
                     /locus_tag="PK105_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOU61557.1"
                     /translation="MAHKFRLLETGFHPAVFNMGLDEAILDSVSQGNCLPTLRFYGWI
                     PPAVSLGYFQGLQEEIDIEACKALGIDVVRRITGGGAVFHHHEVTYSIVIPLGHSLAR
                     SGIIDSYRILLAGIVEGLAELGIQSEFAPINDIVSAGKKISGNAQTRKKGCILQHGTI
                     IVEVDIDLMFSILKVPQEKTKGKLIEDAKSRVTSIRNCLSVPDSVSFEELFFSTITAL
                     ERGYSRALDLDYELSGPTDLELQNAERLAREKFSNNQWTAMR"
     gene            complement(2587..2883)
                     /locus_tag="PK105_07300"
     CDS             complement(2587..2883)
                     /locus_tag="PK105_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61558.1"
                     /translation="MKLELIGKPKGCKLLRMTLEVEPSLSTSSRIISLSIRGDFFAIP
                     EEAFDEIQDELRGITIGEFAECFDRLAQEKALEFEGISGEGLMELIQKELNHGS"
     gene            complement(2861..3754)
                     /gene="lipA"
                     /locus_tag="PK105_07305"
     CDS             complement(2861..3754)
                     /gene="lipA"
                     /locus_tag="PK105_07305"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOU61559.1"
                     /translation="MKNSISDPKPSWLKIRLPHGQNWRHVEEVLASHGLHTVCDEAHC
                     PNKGECWGSGTATFMIMGDICTRGCRFCAVRAARFGRPLDPEEPQHLAKAIESLGLSY
                     AVITSVDRDDLSDRGSAHFSACIRAIRDLSPSTRVEVLIPDYIGQELKEVLSAKPDVI
                     AHNIETVRRLQSVRDQRASYNKSLQTLTEAASTGIPTKSSLLLGLGETEGEVLEALKD
                     LRSAGVSLIVMGQYLQPSPNEIPVKEYISPDRFDRYAEIARVLGFDSVLSAPFARTSY
                     HAREAWNASKKEHKNEARTDR"
     gene            3901..4953
                     /gene="mtnA"
                     /locus_tag="PK105_07310"
     CDS             3901..4953
                     /gene="mtnA"
                     /locus_tag="PK105_07310"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOU61560.1"
                     /translation="MQIPLTMKLDDEKGELIILDQSMLPNEVKYLRLSGTEEIWEAIQ
                     TLRVRGAPAIGIAAAYGAYLAVRNAQPSTMEDAIRIFMEARARLATSRPTAVNLFWAL
                     DRMQKRLEKGISQSSVQRLCTALREEADAIRKEDAQVCRCIGEYSLELLERGWGLLTH
                     CNAGVLATSEYGTALAPIYLGQNRGYGFRVFADETRPLLQGARLTAWELMQAGVDVTL
                     ICDNMAASIMKKGWVQAVLVGCDRIAANGDTANKIGTLGLAILAAHFGVPFYVHAPRS
                     TIDMNCATGLEIEIEERAAEEITTKWYEKPMAPEGVKAFNPAFDVTDAELITAIVTEK
                     GVARQPYDRSLRAMMK"
     gene            complement(4959..6293)
                     /locus_tag="PK105_07315"
     CDS             complement(4959..6293)
                     /locus_tag="PK105_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOU61561.1"
                     /translation="MENLERFSILSQAADWDAAAPSPLIEHPNIRFTDAKRPDNAASE
                     ICWDAAYNICKVKRLGGGSVPILKILLSNICSYNCAYCVNRRSSNIKRASFQPEELAR
                     TITDLDARGLISGAFISSGVIGTPDNTMERLVWTAQTLRERYGYQGYLHIKVIPGASP
                     ELVNLAMRYATRVSVNIELPTEESLIQIAPDKSADAILSPMKTICRRLSELEESNRSK
                     KNLQKTRISNRLAEMSAGQTTQLIVGASPESDATILSLAQHLYRNFNVRRVYYSAFRP
                     EGTDPSLPRLSSPPFEREYRLYQADMLFRWYGFEPAELFEAQDFLDEDLDPKTSWALR
                     HLEFFPVELMTSGYEELLRIPGIGPKTAYKILRMRKNGGLEFSSLKKLRIPTQKIFCF
                     ITIKGKRSSEELHGFRHSFEGQALENPETLREILRERKPPSPPAQQEFDFLS"
BASE COUNT         1691 a         1483 c         1528 g         1797 t
ORIGIN      
        1 gggcttttat gtaagctcat tctctataaa ggctccaagc ttgaggtaat ctctatataa
       61 ggcatcatac agcgatgttc taacaaaatc tggctggata actgtttcta tatcgggctt
      121 cattgccaat tgagcttgcg taacagaatc atagattcct gccactaccg atgcaaacat
      181 tgccgcgccc agggctacgg attgatcgct cgctataatc tcaattgggc gaccaagaac
      241 atcggcgaca atctgaacca ccagtttaga ttttcgcgca actccgccta cagcccgaac
      301 cttctctata tgtatgttct cttgctcaaa acgctctatg atagctcgtg aaccataagc
      361 cgtagcttct ataagtgcac gatatatcat tggcgcagta gtgcccaagc ttaggcctat
      421 gattgcccct tttagtttct ggtttgcata aggagtgcgc cttccattta gccaatctag
      481 agcaagaaca ctggattctt ctggttggat ttttgaagcg gccttatcta ttgcgaatat
      541 gagccgagaa tctatgctgt ctgcaagatc atccagagtg tttgaaggaa tgcccagctc
      601 tttcgctgat tccattgatt cggatttcat ctcttttagc ggccaagaga gaagatcctt
      661 gaaccaggca tagacatcgc caaaagcact ttggccagct tcatatccaa tcattccggg
      721 aatgatagaa ccgtcgacct ggccacatat tccttttaca agatgctcgc tggtctctgg
      781 attttccgaa gatgttcttg gtccaactat catatcgcag gtactggtac ccataacctt
      841 cacaagcacc ttttcttcta ctcctccgcc tactgctcct acatgagcat cgtaggctcc
      901 aaccgccacc ggaataccgg atcgaaggtg aagcctttca gcccattcat tcgaaagctt
      961 tccaaattcg gtatcgcttg tgtaggtatc tcttcccaaa gtctttttga ttcgtactag
     1021 ttgcatatcg agagcagcga aaaaatcatc gctcggatag cctccaaaac tctcatgcca
     1081 catcgcctta tgccccattg cacaacgact tcgctttatt tttgggagag cgttcgttcc
     1141 cgtcagtagc gcgggcatcc aatcacaatg ttctattgca gaaaagccag cattcatgac
     1201 atggcggtct gtgcgggcta tatggagaag ttttgcccag aaccattccg aagagtatgt
     1261 gccgccctca tacatcgtat aatcgacgct ccatcttcgc gccagctcgg ttatctcaaa
     1321 ggcttcttct gtcgaagtat gatccttcca taatatgaac atagcgtcag ggttgtcttt
     1381 atactcaggg agcattgcga gagggcagag ggtatcagtt atgagacagg gcgtagagcc
     1441 tgtggtatcg atagcaatgc cttctacgaa tggcaggaca tcttgcccgg tcttatcgtg
     1501 tatctgtttc attacgatct caagtgactc gatataatcc attggatgct gcctgaattg
     1561 attctttcct ggatcgcaat agagtccctt tgaccatctg ggatatgcag ccattgcgct
     1621 ggatatttcg taaccattag cggcatctat tatcacggct cgacacgaat cggttccata
     1681 gtctatgccg atgacatact gaggcttagt ttcttttttc atcgaaatct ccatgcttca
     1741 atttagctat tataccgaaa gatcgatcaa agcaagggta caagatcgga tcaaaagcaa
     1801 gatttgcgtt tgagctatcg cattgctgtc cattgattgt tggagaattt ttctcgggca
     1861 agcctttcgg cattttgtag ctcgagatct gttggtccgg atagctcgta gtcaagatcg
     1921 agcgcccgtg aataacctcg ttcaagtgct gttattgtag agaaaaaaag ctcttcaaag
     1981 ctcactgaat caggcacaga aaggcagttg cgtatgctag ttacacgtga ctttgcatct
     2041 tcgatgagct tacctttggt tttttcctgt gggactttca agatcgagaa cataagatcg
     2101 atatcgacct cgactatgat ggtgccatgt tgaagtatgc accccttttt tctggtttgt
     2161 gcgtttccag agatcttttt tcccgcactt acgatatcat tgataggagc aaattcagac
     2221 tgaatgccga gttccgcaag accctcgact atgccagcaa gcaatatcct atatgaatcg
     2281 attatacccg agcgtgctag agaatgtcct agaggaatta caatactata tgtcacttca
     2341 tgatgatgaa atacggctcc tcctccagtg atgcgtcgaa caacatcaat gcctagagct
     2401 ttgcatgctt ctatatctat ttcttcctgt aaaccttgaa aatatcctaa ggagacggcc
     2461 ggaggaatcc aaccatagaa gcgcagggtt ggaaggcagt tgccctgtga cacggaatcg
     2521 agaatcgctt cgtcgagacc catgttgaat actgcgggat gaaagcccgt ctccaggagt
     2581 ctaaatttat gagccatggt tgagttcctt ctgaatgagt tccataagtc cttctccgct
     2641 aatgccttcg aattctagag ctttctcttg ggcgagtcta tcgaagcatt cggcgaattc
     2701 tccgatagtt attccgcgga gctcatcctg aatttcgtca aaggcctctt ctggaattgc
     2761 aaagaaatca cctcggatgg aaagggaaat gatcctcgag gatgttgata gggaaggctc
     2821 cacctcgagt gtcatccgca aaagcttgca gccttttggt ttaccgatca gttcgagctt
     2881 catttttgtg ctcctttttt gaggcattcc aagcctcgcg ggcatgatag ctcgttctgg
     2941 cgaatggagc ggacagcaca gagtcaaaac ccagaaccct cgctatttct gcatatctgt
     3001 cgaatctatc agggctgata tattctttta caggaatctc atttggcgat ggctgcaagt
     3061 attggcccat cacaattaga gatacacccg ccgacctaag atctttgaga gcctcgagca
     3121 cttctccttc ggtttctcca agaccgagca ataggctgga ttttgtgggg attcctgtag
     3181 acgcggcttc ggttaaggtc tgcaggctct tattatagga agctctttga tcgcgaacag
     3241 actgcaaacg gcgaacggtt tctatattat gcgcaattac atccggtttg gcagatagta
     3301 cttccttgag ctcctgtcca atatagtccg gaattagtac ttctactcga gtagatggac
     3361 taaggtcgcg gattgcgcgg atacaagccg aaaaatgggc agaaccacga tccgacagat
     3421 catctcgatc cacagaagtt atgacagcgt aggagagacc aagcgattct attgcttttg
     3481 caaggtgttg tggttcttca ggatccagtg gtcggccaaa tctggcggcc cttaccgcgc
     3541 agaagcgaca gccgcgagta cagatatctc ccattatcat gaaggtagcc gtaccagaac
     3601 cccagcattc tcctttattg gggcagtgag cttcatcgca tacggtatga aggccatgac
     3661 ttgccaggac ttcttcgaca tggcgccaat tctggccatg cggaagccgt atcttgagcc
     3721 aggatggctt tgggtcggaa attgagtttt tcatgaatac gtattataga cggatctgct
     3781 aaaaaagtaa tgatggatcg tgattgactc cagattatat aaccctatta ttgttagggg
     3841 cgccttctgt gtaaaaggtg ttttttgatt taaaaaggca ggaaaaggag aaatttcgat
     3901 atgcaaatac cgctgacaat gaagctcgat gatgaaaaag gggaattgat tattctcgat
     3961 caatcgatgc ttccaaacga ggtgaagtac ttacggcttt ctggaacaga ggaaatctgg
     4021 gaagcgattc aaactcttcg agttcggggt gctccggcta taggaattgc agcagcatat
     4081 ggagcatatc ttgcagtccg aaatgctcag ccctcaacca tggaagatgc aatacgtata
     4141 ttcatggaag ctcgagcccg tctcgccact tcaaggccta cggcggtcaa tctattctgg
     4201 gctttggata gaatgcagaa acggctggaa aaaggaatat cgcaatcctc ggttcagagg
     4261 ctttgtaccg cgctaagaga ggaagccgat gctattcgca aggaggatgc acaggtgtgc
     4321 agatgtatag gcgaatactc tctggagctc cttgaaagag gatggggact ccttactcat
     4381 tgtaacgccg gagtcctagc cacctcagaa tatggtacag ctcttgctcc aatttatctt
     4441 ggacaaaatc gaggttatgg ttttcgagta ttcgcagacg agacaaggcc attactgcaa
     4501 ggagctcggt tgacggcatg ggaactcatg caagcagggg ttgatgttac ccttatctgc
     4561 gataatatgg cagcaagcat catgaaaaag gggtgggtac aagcagtact tgtaggttgc
     4621 gatcgcattg cggcaaatgg agacacggcg aataagatag gtactcttgg acttgccatt
     4681 cttgctgcac attttggagt tcctttctat gttcatgcgc cgagatcaac aatagatatg
     4741 aattgtgcaa caggacttga aattgaaata gaggaacggg cagccgagga aatcacgaca
     4801 aagtggtatg aaaagcccat ggctccagaa ggtgtcaagg cattcaatcc agccttcgat
     4861 gtaactgatg ccgagcttat tacggccatt gtaactgaaa aaggtgtggc tcgacagcca
     4921 tacgatcgat cattgagagc aatgatgaaa tgatattatc aagatagaaa atcaaactct
     4981 tgctgtgccg gtggagaggg tggctttctt tctctcagta tttcgcgcaa ggtttctgga
     5041 ttttcaaggg cttggccttc gaaagaatgc cgaaatccat gcaattcctc cgatgacctc
     5101 ttgcccttga tagtgataaa gcaaaagatc ttttgggttg gaattcgcag ttttttcagc
     5161 gacgagaatt ccagcccccc attcttgcgc atccgcaaaa tcttgtatgc agttttaggt
     5221 ccaatgccag gaatccgcag aagttcttca tatccggatg tcatcagttc aacaggaaaa
     5281 aactcaaggt gtcgcaacgc ccatgaagtc ttgggatcga gatcctcatc gagaaagtcc
     5341 tgtgcctcga acaactccgc tggctcaaat ccataccagc ggaagagcat gtcggcctgg
     5401 taaagtcgat attcgcgctc aaagggagga gaggagaggc gcggcagaga tggatcggtt
     5461 ccctccggtc tgaatgctga ataatagact ctccgcacat taaaatttct atagagatgt
     5521 tgagcaagcg aaagaatggt ggcatcggat tcagggcttg caccaactat gagctgtgtt
     5581 gtctggcctg cggacatctc tgctagccta ttgcttattc tggtcttctg gaggttcttc
     5641 tttgatcgat ttgattcctc gagctcagaa agccttctgc agatggtctt catgggagat
     5701 agaattgcat ccgccgattt gtctggcgca atctggataa ggctttcttc tgtaggcagc
     5761 tcaatgttga cagacacgcg agtagcataa cgcatagcca gatttacaag ctctggcgaa
     5821 gctcccggta taacctttat gtgcagatat ccttggtatc catagcgctc acgtagagtc
     5881 tgtgcagtcc acacaagcct ttccatggta ttgtctggag taccaatgac tccagaagat
     5941 atgaaagcgc ccgagataag gcctcttgca tctaaatccg taatagtgcg agcaagctct
     6001 tccggttgga atgaagcccg cttgatgttt gaactacgac gattcacaca gtaagcacag
     6061 ttataagaac atatatttga gagcaaaatc ttcagtattg gtacacttcc cccacctaag
     6121 cgtttgactt tgcagatatt gtaggcagca tcccagcaga tttctgatgc cgcattgtct
     6181 gggcgtttgg catcggtaaa ccttatattt gggtgctcta tgaggggact tggggcggct
     6241 gcatcccaat cggctgcctg tgaaaggata ctaaatctct caagattttc catttactaa
     6301 acacaagttt agtagcagta ttattatctg tcaatccttt tttattgctt tattaaaagg
     6361 agtttttcaa tcccagaata gtctcggcta gttgcagata gcagagaaaa ataaaaaagc
     6421 cctcttttga gggcttagtc ttttttccta gcgggagagg ggattgaacc cactattctg
     6481 aaaatcgata aattttaat
//
LOCUS       DAOOWY010000103         6258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_12597_length_6258_cov_16.105110, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000103 DAOOWY010000000
VERSION     DAOOWY010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12597_length_6258_cov_16.105110"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            601..921
                     /locus_tag="PK105_07320"
                     /pseudo
     CDS             601..921
                     /locus_tag="PK105_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(988..2493)
                     /locus_tag="PK105_07325"
     CDS             complement(988..2493)
                     /locus_tag="PK105_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOU61562.1"
                     /translation="MEMIFSGIAQGMAQLLDPVVLALIAAGTIFGIVIGALPGLTSTM
                     GVALIVPITFGMTPVQGLSLLGAIYCSSTYAGAISAILINIPGTPANCCTVLDGFPMT
                     KKGQAGLAIALATVASAIGGIISNVALLFLAPPLADLALRFGAQEYFLLALFGVSVIA
                     SLSERNMLKGFISGTLGLFLSIVGMHPLTGDIRFTLGIPELFNGLPLVVALIGLYSIP
                     EVIANLSEVGSDEGMSFVEVKGVWRQMLEVFKYKMVLLRSTIIGIIVGIVPGAGSSIA
                     GFIAYDDTKKKSKNPETFGTGNPAGVVASETANNAVVGGSLIPSLTLGIPGNAVSAVL
                     IGGLMIHGLKPGPALFTENSGTIYGFILSMFISNIVFIPVGLLIARYGIKIIKTPPSI
                     LGPLVIGLAVIGAYALNMSLVDVWIMLAIGLMGFAMKEFDVPREPLVLGLVLGSMAEG
                     ELARSMALVQGSVPALLLSMVTRPISLIIILLTALSIFKGLHSQLKPKKSA"
     gene            complement(2522..2989)
                     /locus_tag="PK105_07330"
     CDS             complement(2522..2989)
                     /locus_tag="PK105_07330"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOU61563.1"
                     /translation="MAKISRKKEIGFAAGMTFISAVFLVQSYLYPAESSQFPRFIMML
                     QTAFSAVLLVRTMKQPKVVGGRKAKTEGSRKSLISEFKVPLEIFLASSAYLFAIDALG
                     YFVASSIFLAGTMYLFGARKPIVIIGATAGFLLMVYALFVLFIGVRLPQGLLI"
     gene            complement(3009..3980)
                     /locus_tag="PK105_07335"
     CDS             complement(3009..3980)
                     /locus_tag="PK105_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOU61564.1"
                     /translation="MKLTPKSRIAATLILLALLGSAVPTFAQAAFPTKPIVCIVPFSA
                     GGGNDILIRLLSKYANRYLGQTLVVENKPGASGQIGWTALAKSKADGYTLGATSLPSM
                     ILIKAIRPATPFELDDFRYICNIQIDPIIWVVKSDSRFKTAKDFVEQAAAKKLNVAGD
                     GPQSNVQLQHLAATKALGIQTNFISYSGSGPAITSLLGGQVELAATTLSSAIPNIEAG
                     KLRALVVFSEDPIDALPGVPLASAYFGKTIPSVGMAIRGLAAPKDIAADRIAILEKAF
                     RQICESKEFLEEAKGLGIIVKFQGSAEAEAIVKSSITQVEELKELLQ"
     gene            complement(4023..4997)
                     /locus_tag="PK105_07340"
     CDS             complement(4023..4997)
                     /locus_tag="PK105_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61565.1"
                     /translation="MKMTEALMFKGAKTILDDCVVAKKGESLFIVTDANKLHIAQVLF
                     AAALERGLEATIGIIEPREKAGQEPPKLIALGMKSADIVLCPVSKSITHTFAVKEAAQ
                     AGARILVMTDFTDETLIRGGIEADFKAARTVCRGMADAFSKGKSLHITSPGGTDLSVD
                     ITGRRGNALYCIVEPGELSTVPTVEANVSPVEGSARGRIVADASVPYLGIGLLNEPVE
                     AMVEAGYITSIKGGKQATALLRDLESHGDRNCFNIAEIGLGLNPKCRMIGVMLEDEGV
                     VGSAHIGIGTNITLGGVIKAPTHYDLLMWNPTITIDGVKVFEGTKVLI"
     gene            5805..6029
                     /locus_tag="PK105_07345"
     CDS             5805..6029
                     /locus_tag="PK105_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61566.1"
                     /translation="MIIGDGAKWIWALAYELFPHGIQIVDLYHALETVSTVAKKIFGI
                     ESKEGDQKAQSWRELLERGAIDEIIGALTL"
BASE COUNT         1622 a         1523 c         1637 g         1476 t
ORIGIN      
        1 agctcgatat catcgaatgc tatgcccatc ctgggcatag gcttcaggta ggagagatca
       61 ccaataagca gaaatttctc tatgaggcgc tcggagttga gtctccctca ttggtataaa
      121 tgttcgggaa tccggggtgc acatcatgtt ctctttatgg atgaagatat ggagaactat
      181 acatgcacaa ttctctcaag ctgatgcgtg gaggagatac gataaaatgg agtgctgaag
      241 agttcgatat gtcgaagcgg ccgattttct ccttcaccaa aaactattgc ccgaatggta
      301 aaaatctgag taatgcccga tactggcata gtctgtgact atgcgaagaa ttcgccctaa
      361 agaatcgtgc ctttgtgaat aatgcagaat tgctcaattt tctctaattt cagcatctct
      421 cctgcggaaa tttggcaaaa atccgcagca tagtagcgga ttttatgaat tatggatacc
      481 ttcggcgcct gtgaggggaa agggctcatt tcgtctaaga ggatgggcgt ctcaagaagg
      541 catgagcttc ttgagggcga gcgcgttatg aagagcgaag tgtgaaacct cgctagtgag
      601 catggtatgg aggtagcggg gaaacctgaa agtcaggatt tcgttgcggg tggagattat
      661 gctccacttt tcgaaaacgc cgaacctcta cccggcgact tcgtcgatac gacaggtaaa
      721 atattcggaa aacacaaagg cctgcccttt ttttacacaa ttggccagca gagaggcatc
      781 ggcctcagta ccggccccat ggctcgccaa gatcagacag aatcacaagc cagtcccatg
      841 cgcggtctac tgtcgccccg gaaccgaggg cgtcgcctcc gtggtgctcg cggaatgatt
      901 gcgagccctt tgttttattg agccaaatat aaaaaagccg ccggaacttg tccggcggct
      961 tttttcatcg atggcagtcg agagttgtca tgccgatttc tttggtttaa gctgtgagtg
     1021 taagcccttg aagatcgaca gggcagtgag caggatgatg atgagcgaga ttggccgtgt
     1081 gaccatgctc aacagcagag cgggcacgct gccctgaacc aaggccatgg agcgagcaag
     1141 ctcaccttcc gccatacttc ctagcacaag gcccaacaca agaggctctc ggggcacgtc
     1201 gaattccttc atggcgaaac ccatcaaccc gatcgccaac ataatccata catccacaag
     1261 ggacatattg agagcatagg caccgatgac ggcgagtcct atgacgagag gaccgagtat
     1321 cgaaggcggc gtcttaatga tctttatgcc atagcgggcg atgagaagtc ctacaggaat
     1381 aaaaacgatg tttgatatga acatgctcag gataaagccg tatatcgtac cggaattctc
     1441 ggtgaaaagc gcaggaccgg gcttgaggcc gtgaatcatc aaaccaccaa tcagcacggc
     1501 ggagacggcg tttccgggaa ttcccaacgt tagggaagga atgagagacc cgcctaccac
     1561 ggcattgttg gcagtttccg acgcgactac tcctgctgga ttgccagtac cgaaggtttc
     1621 tggattcttg gactttttct ttgtatcatc ataggcgata aaaccagcga tgctcgagcc
     1681 tgctcccggt actatgccaa cgatgatacc gattatcgtc gaacgcagaa gtaccatctt
     1741 atacttgaac acttccagca tttgcctcca cacgcccttc acttccacaa aggacatgcc
     1801 ttcatcgctg ccgacttccg aaagattggc gataacctca ggtatcgagt agaggccaat
     1861 caaggccacg acaagcggca agccgttgaa gagttcggga atgcccaggg taaaacggat
     1921 atcgccggtg agaggatgca tgccgacgat ggaaagaaaa agtcccaagg ttcctgatat
     1981 aaaacccttg agcatgttcc tttctgaaag cgaggcaatg acggagacgc cgaaaagcgc
     2041 tagaaggaag tactcctggg cgccgaatct caaagccaga tcagcaaggg gcggagcaag
     2101 gaatagcagt gcgacattgc tgataatccc gcctatcgcc gaggctactg tcgctagcgc
     2161 tatggcgagc cccgcctggc ctttcttggt catgggaaaa ccgtctagga ccgtgcagca
     2221 gttggctggc gtgcctggaa tattgataag aatggcgctg atcgccccgg cataggtcga
     2281 tgaacaatag atggcaccga gaagcgataa gccctggacg ggcgtcattc cgaaggtgat
     2341 gggcacaatc agggcaacac ccatcgtact cgtaagccca ggcaaggcgc cgatcacgat
     2401 gccgaatata gtgccagccg ctatcaaggc cagcaccaca ggatcgagca gttgagccat
     2461 accctgagca attccactga aaatcatctc cattagaaaa cctccaattg ccccgtgccg
     2521 cctagatcag caggccttgc ggtagacgca ccccgataaa aaggacaaag agcgcatata
     2581 ccatgagaag aaatccggca gtcgctccta tgatgacgat tggcttgcgg gcaccaaaca
     2641 agtacatggt tccggcgagg aaaattgacg aagcgacgaa atatcccaga gcatcaatcg
     2701 cgaaaagata ggccgaggat gcaaggaaaa tctctaatgg gactttgaat tcggagatga
     2761 gactttttcg ggaaccctcg gtcttcgcct tccttccacc tacaaccttg ggctgcttca
     2821 tggtcctgac cagaagaaca gcactaaatg ccgtctgcag catcatgata aacctaggaa
     2881 actgggatga ttccgcaggg tataaatatg actgcaccag gaaaaccgcg gagatgaatg
     2941 tcatccctgc ggcaaaccca atttccttct ttcgggaaat ctttgccata gtagattcct
     3001 ttgctcgtct actggagcag ttctttcagc tcttcgacct gtgtaatcga tgattttacg
     3061 atagcttcgg cttcggcgga accctggaac ttgacgatga tgccgagccc cttcgcctct
     3121 tccagaaatt ccttggattc gcagatttgc ctgaatgcct tttccagtat ggcaatcctg
     3181 tccgcggcga tatccttggg agccgccaga cctcggattg ccatgccaac cgaggggatc
     3241 gtctttccga aataagcgga agcgagcgga actcccggaa gcgcatcgat aggatcttcg
     3301 ctgaagacta caagagccct aagttttcca gcctcgatat tcggtatggc cgaactcaga
     3361 gtcgtggcgg ctagttcgac ctgtccgcca aggagcgacg taatcgccgg accagagccc
     3421 gagtaggaga taaagttcgt ttggatgcca agggccttcg tggcggccag gtgctggagc
     3481 tgcacattgc tctgaggccc atcgcccgct acgttgagtt tcttagccgc cgcctgttct
     3541 acgaaatcct tggctgtctt gaagcggcta tccgacttca ccacccagat gatcggatcg
     3601 atctgtatat tgcagatata cctgaagtcg tcgagctcaa agggggttgc cggcctgatg
     3661 gctttgatca ggatcatgga aggcaagctc gtcgcgccca gggtatagcc gtcggccttg
     3721 gacttggcga gagctgtcca tccaatctgt ccgcttgctc ccggtttgtt ctcgacgacg
     3781 agggtttggc caagataacg gttggcgtat tttgaaagca gcctgatcag gatatcattg
     3841 cctcctccag ccgagaaggg aacgatacat acgatcggtt ttgtggggaa agccgcctgg
     3901 gcgaaggtcg ggacagccga acctaacagc gccagaagaa tcagcgttgc agcaatccgc
     3961 gatttaggcg tgagtttcat ggaaaacctc ctatatgaaa tgcatggcgg cgcgatagcc
     4021 gctcagataa gaacctttgt accttcaaac accttgacgc catcgatcgt tatggtcgga
     4081 ttccacatga gaagatcata gtgagtcggc gccttgatga ctccgcctaa ggtgatgttc
     4141 gtccctatgc cgatatgggc cgaacccacc actccttcgt cctcgagcat tacgccgatc
     4201 atcctgcatt tcggattcaa gcctaagcct atctcagcga tgttgaaaca atttcgatcg
     4261 ccatgactct caagatcccg cagaagagcg gttgcttgct tgccgccctt tatgcttgtt
     4321 atataccccg cctcaaccat cgcttccact ggttcgttga gaagcccgat tccgaggtac
     4381 ggaacgctcg cgtcggcgac gattcttccc ctggctgagc cttccactgg ggatacattc
     4441 gcttctaccg tgggcaccgt cgataattcg ccaggctcta cgatgcagta gagggcattt
     4501 cctcttctgc ccgtgatatc gacagataga tcagtaccgc ccggcgaagt gatatggaga
     4561 gatttgccct tcgaaaaggc atcggccatc ccccggcata cagtcctcgc agccttgaag
     4621 tcggcttcga tgccgcctct gatgagagtt tcgtcggtaa agtcggtcat caccaggatt
     4681 cgtgccccgg cctgggccgc ttctttcact gcgaaggtgt gcgttatgga tttgcttacg
     4741 gggcaaagca cgatatcggc gcttttcatg cccaatgcaa tgagctttgg aggctcctgg
     4801 ccagccttct cacggggctc gatgataccg atcgtagcct cgaggcctcg ttccagcgcc
     4861 gcggcaaaaa gcacttgggc aatgtgaagc ttattcgcgt ccgtgacaat gaagagactc
     4921 tcgcccttct ttgccacgac gcaatcatca agtatcgttt tcgcgccttt gaacataagc
     4981 gcttctgtca ttttcatcgc caagtctccc tctccgcgaa ctgaatttat cgttatttta
     5041 agactaatta ttatataaaa aagattataa tacgataata ctacaaagtc aagaatttta
     5101 ttttgggtgg gtgcacgaca aatttgtgat gatttttcta aggggagatg aaagaaagac
     5161 ttgctttttt ctatctggaa atatattaga tgttcagata attggaggca tctataacga
     5221 atcagattga gtcgttggaa gagtttgtgc agaaaaagaa gatagtattg gaagaaatag
     5281 gaaagcttcc tgcctttagg cctggtagcc tagaacatat gtataggaaa tgcggcaagc
     5341 caaactgcca ctgtgcacag cctggtgcaa aaggccatgg accggtctgg attctcacaa
     5401 ggaaagtaaa aacaagactg tgtcgaagac gatcaagaag gatgcagtgg agttaacgca
     5461 tgcacagatt aaaaactatc atgagtttca ggagctcata cgtgagtatg tggagaagaa
     5521 tgtgaaaatc tgtgacaagc ttctagagca gtcaaggtag acagcctcag taaggctgaa
     5581 aaagagtgtg gcaatggcgt ttatccttgc gataaggaac ttgggctcac cttggggctt
     5641 ctgtcaccag gagtagagat gttgcagagg atgaatgctc ctatgtccct gtgagagagg
     5701 cagtacaaaa aactatgtac cttggacttg atgggacagg gataccgatg cgccatgttt
     5761 aacgggaagc ccatcggact ggctttgaga atgctccttg gcaggtgatc attggtgatg
     5821 gagcgaaatg gatatgggcg cttgcgtatg aactctttcc tcatggcatt cagattgtgg
     5881 atctctatca tgccctagag acagtctcta cagtcgcaaa gaagatattt ggaatagaga
     5941 gtaaggaggg tgatcaaaaa gcccaaagct ggcgggagct tctagaaaga ggagccattg
     6001 atgagatcat cggagcactt actctatagg aaccaggctt aagcgatcgg ggatgcattg
     6061 gacagtcgaa ggtgccaatg acattattgc gctgcgatgc aataggctta gtcaccgtga
     6121 tgaggagtat tgggctcgaa gaaatgttac ttagctacct ttgcacataa ttgtcgtgca
     6181 ctcattttat tttgtcattt aagaatgagt tcactctaaa aaggtctctc aaaaggcttt
     6241 ttggtaagct atttatat
//
LOCUS       DAOOWY010000104         6129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_12935_length_6129_cov_8.835693, whole genome shotgun sequence.
ACCESSION   DAOOWY010000104 DAOOWY010000000
VERSION     DAOOWY010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12935_length_6129_cov_8.835693"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PK105_07350"
     CDS             <1..877
                     /locus_tag="PK105_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOU61567.1"
                     /translation="ICINYGKLNEVAQVKVGSGFIIMLSNPGAYRTQYEIRKLVRSNK
                     REDYASDEIFANFRNIAIGNIAEGVLYRSSSPIDNQLGRAAYVDKLIEEAGIQTIVNL
                     SDSIDAMKTYLAAKDFASPYYAELAKNNKVIFLNMNLAYGSEEFRANVVKGLVFMVEH
                     DGPYLVHCTEGKDRTGFISALLAALMGATKDEIVADYMESYINYYGVEPGTEKYNLIA
                     QDVLDMLKVITGNSDLEKADLEAGARNYLILGGMKPAQIEQLMNKLSKPLSSLESLEF
                     TAFFLARIDEYLMIA"
     gene            complement(846..2060)
                     /locus_tag="PK105_07355"
     CDS             complement(846..2060)
                     /locus_tag="PK105_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU61568.1"
                     /translation="MKNRRQAAYRFLVLLGVVSLFSDLTHEGARSILGPYLLLLGASA
                     STVGFVSGLGEFIGYALRLVTGYISDKTKRYWLITIIGYSINLIVIPALALAPGFGWI
                     YACVLIVLERFGKAIRNPAKTTIVSFAATEVGAGKGFALQEVLDQIGAFAGPLMLFLV
                     LTLKGNDNKISAYQLSFALLAIPAAVALILLLLARKNYPEPQEFEKPNEQTLPQNGLN
                     SAYWYYLVGIGFLALGFADFPLMAFHMTKTELLPDNMVPVLYSVAMGIDALSALYFGR
                     MYDRRGITAIIISSALALFFAPFIFLVGTPGGAIAGIMLWGIGMGAQESILKSAVTTI
                     VPKERRGTAFGIFNAGFGAFWFAGSWIMGLLYERSLLALVLFSMIGQLIALPFFFKTR
                     SILKQSSNIRQS"
     gene            2355..3593
                     /locus_tag="PK105_07360"
     CDS             2355..3593
                     /locus_tag="PK105_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU61569.1"
                     /translation="MKSKKAKAIFFMVFFVTSLIILGAQQKTTLSVLWFNDANESDVF
                     MATMNDYQKAHPNIVLDMQVIPFNDYENKLRMMIAGGNPPDVARLASAHVPLFATSLE
                     PLSGKSEFDELAKSYFDSSLALGMDENGRIMAMPTEATANGMIVNKTYFKNAGIDIDK
                     LSLTWTWDQWVDAMKKVIKANPKAKYGITVDFSTHRFATFLYEAGGRFLSSDGKSMNF
                     NTPESLDALKFFKMLHDEGLAPKSVWMGSENPQELFQSGLVACHVGGSWLINAYNKNI
                     KDFEWAAVRMPKRKINSSVPGGKFIGTFKSSAHKKEALDLIAVFSDKAHNEQYCKDTF
                     NLSARKDANIKYTARSNDFAVFTAELNVTPPYTADDWKSDAVAKVNSYAREQIVEGLL
                     GKQTMEQTAANIQAKGNSYF"
     gene            3734..4627
                     /locus_tag="PK105_07365"
     CDS             3734..4627
                     /locus_tag="PK105_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU61570.1"
                     /translation="MKSKQRSHGTFEQTLAPLLFLTPNLLIFSIFIIVPAIMGLRMSF
                     FDWSILEGSRFIGFQNFKSISHDSMFWLTLKNTLVYVGAVVPLLTVFALALALPLSKE
                     DRGMGVFRVIYYIPTMLSMIVVGISWRWLLGYDLGIINYVISLAGGKPVPWLTNSTMA
                     NICLIFVTLWTRVGYFMMMFVGGLQAIPVTYYEAAKMDGAGRSTIFRRITLPLLSPTI
                     LVVVVLATIEAFKAYELIFVMTQGGPGTSTKFIVQYIYQVAFQEDKMGYGAAMSVILL
                     IIIGIFTALQFSIQKEEYTNE"
     gene            4620..5429
                     /locus_tag="PK105_07370"
     CDS             4620..5429
                     /locus_tag="PK105_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU61571.1"
                     /translation="MNRALKYILLIIVSGIFLFPILWVIISSFKPQSELFTYPLTILP
                     HKATIENYVNAFASGDFITYYANSIFVSVAATLLTITINVMAGYALSKFYFKGRDLIF
                     YIMISTLMIPLQAIMIPIFLLLKELGLLNSLWGIIIPPAATPTGVFLARQYIQTIPNS
                     YIEAARMDGASELRIFLRIIVPLAQPIIATIAIFSFMWRWNDFLWPFIVISSKRKMTL
                     QLALANFVGQFQINWGNLLAMTVVTMIPMIVVFLVLQRYFVQGLSAGGVKA"
     gene            5432..>6129
                     /locus_tag="PK105_07375"
     CDS             5432..>6129
                     /locus_tag="PK105_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61572.1"
                     /translation="MLYVIKNLSSYHLEGSKAIFSGENGQLEVVIYASNLFSIFYVFD
                     ESIMPPEMSIPHSTLLSLNDLDDAISWDCVIENGFQYELKHGSTIILIDKATSRVDVY
                     ENNVLFHGGEIGHKDLVVPQYPLRVQLGDTPSNGRQDIYGKFNFRLEQEDAFFGLGEK
                     TGKLNKRNRLFKLFNRDALGYNAETSDPLYKSVPFFIRTNRVLNNMCGLYFPNLRVDE
                     VNFGVESEFYYDVVL"
BASE COUNT         1755 a         1283 c         1359 g         1732 t
ORIGIN      
        1 tatatgcatc aactatggaa aactgaacga agtggctcaa gtaaaagtag gcagcggctt
       61 tatcattatg ctgagcaatc caggagccta tcgaacgcaa tatgaaattc gcaagctggt
      121 gcgctccaac aaaagagaag attatgcttc cgatgaaata ttcgccaatt tccgcaatat
      181 tgccatagga aatattgcgg agggtgtcct ctatcgcagc tcgagcccta tcgataatca
      241 gcttggaagg gcggcctacg ttgataagct gatcgaagag gcaggtatcc agacgatagt
      301 caacttgtcg gactccatag acgcaatgaa gacctatctt gcggccaagg attttgcatc
      361 gccttattat gcagagcttg caaagaataa taaggttatt ttcctcaata tgaatctggc
      421 ctatggaagt gaagaattca gggcgaacgt agtaaaaggc cttgttttca tggtcgagca
      481 tgatggtcca taccttgtac attgcaccga aggaaaggac cgcacaggct ttatctccgc
      541 gctattagcg gcgctgatgg gtgcaactaa ggatgagatc gtcgccgatt atatggagag
      601 ttacatcaac tattatggag tcgaacctgg taccgagaag tacaatctga ttgctcagga
      661 tgtactagat atgcttaagg tgattacagg aaacagtgat ctcgaaaaag ccgacctgga
      721 ggccggagcg cggaattatc tgattttggg aggtatgaaa cctgcacaga tcgaacagtt
      781 gatgaataag ctctcgaaac ctttaagctc attggaaagt ttggaattca cggcattttt
      841 cttggctagg attgacgaat atttgatgat tgcttaagaa tgcttcttgt tttgaagaag
      901 aagggaagcg cgatcagctg gccaatcata gaaaaaagga caagagcgag caaggatctt
      961 tcgtatagta agcccatgat ccagcttccc gcaaaccaga aagccccgaa ccctgcattg
     1021 aaaatgccaa aggccgtgcc tcgacgttcc ttgggtacaa tcgttgttac ggctgatttt
     1081 agtatagatt cctgagcgcc catgccaata ccccatagca ttatgcctgc aatggcgcca
     1141 ccgggggtcc ccacaagaaa tataaatggc gcaaaaaaca aggcaagagc cgaagaaatg
     1201 attattgccg taattcctct tcgatcatac attctgccaa aatatagcgc cgagagggca
     1261 tcgataccca tagccaccga gtacaggaca gggaccatat tatccggtaa gagttctgtc
     1321 tttgtcatat gaaaggccat gagaggaaaa tcagcaaagc caagggcgag aaatccgatc
     1381 cctacaagat agtaccaata tgctgaattt aggccatttt gaggcaaggt ttgttcattt
     1441 ggcttctcga attcctgcgg ttccggatag ttctttcttg caagcaaaag caagatcaat
     1501 gcaacagctg caggaatagc aagaagggca aaactgagct ggtacgccga aatcttatta
     1561 tcgttccctt ttagtgtgag aacaagaaag agcatcaatg gtcctgcaaa agcaccgatc
     1621 tgatcgagta cttcctggag agcaaagccc ttgcccgctc cgacttctgt ggcagcaaag
     1681 gagacaatcg tcgtctttgc gggatttctt atagcctttc caaagcgctc gagcacaatc
     1741 agcacgcagg catatatcca gccaaagcca ggcgctaacg caagtgcggg tatgactatc
     1801 aaattgattg aatagccaat tattgtaatg agccagtatc gctttgtctt gtcgcttata
     1861 taacccgtta caagacgcag agcatagcca atgaattctc caaggcccga tacgaagccc
     1921 actgtcgatg ctgaagcgcc caatagtagc aagtacggcc ccagaatact ccgtgctcct
     1981 tcgtgtgtca gatcggaaaa gaggctcacg acaccgagca acaccagaaa cctgtaagca
     2041 gcctgtcttc tgtttttcat gttaggttcc ttcttgcata atatcctgac aaaagaacct
     2101 tgaccagctg ctgatcgaag tatcggattc tacaaaattt gtggaaacac aatatagaga
     2161 attcgattat agctcgattt tgtaataagg ctgatcgagc agcctcttat gtattgatga
     2221 caatttggct gcgtggcgat attgacggaa ttttgttagg atgcatctct tttgaacctt
     2281 gacaaatatg aaaagtcgct ttatcatgat aatagtcaaa acgttttgac aataagatta
     2341 tgggaggctc aattatgaaa tccaaaaaag caaaggcgat ttttttcatg gttttctttg
     2401 tgacttcttt gatcatctta ggagcgcaac agaaaacaac tctctctgtg ctgtggttca
     2461 atgatgccaa tgaatccgat gtatttatgg caaccatgaa cgactatcag aaggctcatc
     2521 caaacatcgt cctcgatatg caggttatac ccttcaatga ttatgagaac aagcttcgaa
     2581 tgatgattgc gggtgggaat cctcctgatg ttgcaagatt ggcaagcgca catgttccgc
     2641 tttttgcaac aagtcttgaa cctttgtctg gcaagtccga attcgatgaa ctagcaaaat
     2701 cctattttga ttcttctctg gcgctgggca tggatgaaaa tggtcgaata atggctatgc
     2761 caaccgaagc aacagcgaac gggatgattg taaacaagac ctatttcaaa aatgctggta
     2821 tagatattga taaattgtca cttacatgga catgggatca atgggtagat gccatgaaaa
     2881 aagttatcaa agcaaatcca aaggcaaaat atggcataac agtggatttt tcaacacatc
     2941 gatttgctac cttcctatat gaagctggcg gacgattttt gtcgagcgac gggaagtcga
     3001 tgaatttcaa tacgccggag tctctcgatg cgctaaaatt cttcaaaatg ttgcatgatg
     3061 aaggtcttgc ccccaaatcg gtgtggatgg gctcggagaa tccccaagag cttttccaat
     3121 ctggcttagt agcctgtcat gtgggcggtt catggctcat aaatgcctat aacaagaata
     3181 tcaaagattt tgagtgggca gcggtaagaa tgccaaaacg gaagatcaat tcctccgtac
     3241 ctggtggcaa attcattggt accttcaaga gctcagctca taaaaaagaa gcattggatc
     3301 tcatagcggt cttttctgac aaagcgcaca acgaacaata ctgcaaagat actttcaacc
     3361 tgtccgcacg aaaagatgct aatatcaagt ataccgcaag atccaatgac tttgccgtct
     3421 ttactgcgga actcaacgta acccctccct atactgcgga cgattggaaa agcgacgctg
     3481 tggcgaaggt caactcctat gcgcgagagc agatcgtaga agggttgtta ggaaagcaga
     3541 caatggagca gactgctgcc aatatacagg ccaaaggcaa ctcctacttc tgataaaatg
     3601 ttcgttattt ctaaattgac gtaagaattt atcttacgat agatttttaa ttgtttctgc
     3661 tgcatgcatt gaacaagaat gcatgcagca gcctgttttt tatagaagat caactaatac
     3721 agagaaataa aggatgaaat ctaagcaacg atctcatggc acattcgagc aaacgctagc
     3781 acctctctta tttcttacac cgaatttgct gattttctca atattcatca ttgtcccggc
     3841 tatcatggga cttcggatgt cgttttttga ttggagcatc ttagagggtt ccagatttat
     3901 tggtttccaa aacttcaaga gtatatcaca tgattcgatg ttctggctta ctctaaagaa
     3961 tactcttgtc tatgttggtg ctgtcgttcc tcttctgacg gttttcgctc tagctctagc
     4021 gttgcctctg tccaaggagg atagaggcat gggtgttttt cgtgtcatct attatatacc
     4081 gaccatgcta agcatgattg tagtgggcat ttcctggcga tggctcctgg gctatgatct
     4141 tggcattatc aattatgtaa ttagcctggc gggaggaaag cccgtaccat ggcttactaa
     4201 tagcacaatg gccaatattt gcctcatttt tgtaacgctt tggacaaggg taggatattt
     4261 tatgatgatg ttcgtgggtg gacttcaagc gattcccgta acctattatg aagctgctaa
     4321 aatggatggg gcagggagat cgactatttt ccgacggatc acgcttccac ttttaagtcc
     4381 taccattctt gtcgttgtag tgcttgccac catcgaagcc ttcaaggcat atgagctcat
     4441 ttttgtgatg actcagggcg gacccggaac atccaccaaa ttcattgttc agtatatcta
     4501 tcaggttgcc tttcaagagg acaaaatggg atatggcgct gccatgtcgg taattcttct
     4561 tattataatt ggaatattca cggctcttca gttcagcatc cagaaggagg aatataccaa
     4621 tgaatagagc actcaaatat atactgttga taatagtctc aggtatattt ctattcccta
     4681 ttctatgggt cattatctct tctttcaaac ctcaatcaga gctttttact tatccactga
     4741 ccattctgcc tcacaaggca acgattgaga attatgtcaa tgcatttgca agtggtgatt
     4801 ttatcaccta ttatgcaaat tccatttttg tctctgttgc tgcaacattg cttaccataa
     4861 caataaacgt tatggcaggt tatgctcttt ccaagttcta tttcaaaggt cgagatctga
     4921 ttttctatat catgattagc acgctgatga ttcctttaca ggcgattatg ataccaattt
     4981 tcttgcttct gaaagaatta ggtttgctaa attctctgtg gggaatcatc attcctcctg
     5041 ccgcaactcc gaccggtgta tttcttgccc gtcaatacat tcagacaatt ccaaattcgt
     5101 atatcgaggc ggcacggatg gatggcgcaa gcgaattgcg cattttttta aggatcattg
     5161 taccattagc tcagccgatt atcgcgacaa ttgcaatctt ttctttcatg tggcgatgga
     5221 atgactttct ttggccattc atagttattt catctaagag aaaaatgact ttgcagcttg
     5281 cactagccaa ttttgtgggg caattccaga taaattgggg caatctactt gcaatgaccg
     5341 tagtgacaat gatacctatg attgttgttt ttcttgtctt acagcgatac tttgtccaag
     5401 ggctcagcgc cggcggagta aaggcataaa tatgctatat gtcataaaaa atctcagttc
     5461 atatcatctc gaaggttcta aagccatatt ctcgggcgag aatggtcagc tcgaagtagt
     5521 gatctatgct tccaatttgt tttcgatttt ttatgttttc gatgagtcaa ttatgccgcc
     5581 agagatgagc attcctcatt cgacattgct ttccctaaat gatctagacg atgctatatc
     5641 ctgggattgt gtcattgaaa atggctttca gtatgagcta aagcatggct caacaataat
     5701 tttgatagac aaggcgacga gcagggttga tgtttatgaa aacaacgtgc tctttcacgg
     5761 aggcgagatt ggccataagg acctggtggt tccgcagtat cctcttcgag tgcaactggg
     5821 agatacacca tccaatggaa ggcaggatat atatggcaaa ttcaattttc gccttgaaca
     5881 agaggatgcc ttttttggtt taggagaaaa gaccggaaag ctcaataagc gaaatcgact
     5941 attcaaattg ttcaatagag atgcgctcgg ctacaatgcc gaaacaagcg atccgctcta
     6001 caaatcagtg cctttcttta ttaggaccaa tagagttctc aataatatgt gtggcctcta
     6061 tttcccaaat ctgcgagtcg atgaagtcaa ttttggagtc gaaagcgaat tctattacga
     6121 tgtcgttct
//
LOCUS       DAOOWY010000105         6114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_12980_length_6114_cov_9.975078, whole genome shotgun sequence.
ACCESSION   DAOOWY010000105 DAOOWY010000000
VERSION     DAOOWY010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12980_length_6114_cov_9.975078"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PK105_07380"
     CDS             <1..916
                     /locus_tag="PK105_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61573.1"
                     /translation="TNYLMATLHPLISMTQLSNNWANGLASSKRIAEVLDAEPEVVEL
                     PNASPLPSSIHGEVEFDHVYFHYNGDTALKVLEDICTRAEARKVTAVLGATGSGKSSL
                     VNLIPRFYDARSGKVNIDSNDVRLLRISSILEHIAIVPQETVLFSGTVRDNIRYGKPD
                     ASDEEVLAAAVAAHANDFIMDMPEHYETHIEERGANLSGGQKQRIAIARALITKPSIL
                     ILDDATSAIDVETETKIHAAIAKATMGITLIMVAQRISTVLNADKIIVLDEGRIVAEG
                     THRELLKKSPIYKEIYDSQLGAGVRHGL"
     gene            906..2813
                     /locus_tag="PK105_07385"
     CDS             906..2813
                     /locus_tag="PK105_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61574.1"
                     /translation="MAYETSPSIQDVRTSFQRRGGRPTRYERASDPRKAMKRLFGYLM
                     PFRQPLLLIVFFVLLQSLLGLVGPYLLGSAIDKSIGGKDINGLARNAVLMLIAYFLSN
                     SFNLIANRKMAIISQNALKNLRRDLFVHIQWLPMRFFDTNPAGGLMSRLTNDIDAINQ
                     AVSQNIISLVAGVITMSGILVSMFVLNHWLALTSLIVIPIMYFFSNFVAKYTRKGFRE
                     LQKELGDLNAIAEETISGFKVIKAFRRNDSAIETFKRKNEAVYKAAIYANSYALLLMP
                     LTGVIGSFFVILLASLGGWLALKDLVSIGMIATFINYAQNFTQPLRQISNLYNSIQAA
                     LAGAERVFEIIDMDPEIAEEPRLEEAKTNPETAIRVSEDSIIGSSTAPMVFSLGQFQG
                     NVSFKNVVFGYLAGKPVIKNFDVDISAGLTVALVGPTGAGKTTITNLLMRFYEIDEGQ
                     ILFDGVDIRTIPKEAVRRAIGLVLQDTFLFADSVLENIRFGRLDATDEECIEAAKMAE
                     VDHFIRQLPEGYQTNLSERASNLSQGQRQLLSIARAILADPAILILDEATSSVDTRTE
                     IRIQRALLRLMHGRTSIVIAHRLSTIRDADKIIVIDDGKIVEIGDHQALLAKRGFYHR
                     LYMSQFKGNEI"
     gene            2854..3375
                     /locus_tag="PK105_07390"
     CDS             2854..3375
                     /locus_tag="PK105_07390"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOU61575.1"
                     /translation="MNRQDIPLKDFHYDVARLRDHWFALFAGDFNARVFNAMTISWGS
                     YGHVWDKMVFQVMVRPSRYTYDFMNRYDSFTLNAFTPEYKQALSILGSNSGRNLDKIA
                     LAGLTPMASRKVDAPSFEEAQLVIECRKIYWHDLNEANFLDSDIEEHYPRKDYHRIYL
                     GEIVNVSQQIIKP"
     gene            3527..4279
                     /locus_tag="PK105_07395"
     CDS             3527..4279
                     /locus_tag="PK105_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61576.1"
                     /translation="MNLLEIKNITLLKESRPILNNLSMEIWNGHVHAIIGPNGAGKST
                     LANTIMGLSGYRDFQGDVIFNGESLKDLSIDERARRGITLLFQEPARFEGLSVSRFIL
                     AGAKEKSQKVVEDALRQVGLVPENYINRAVDKTLSGGERKRIELASIYAMKPRLVLMD
                     EPDSGVDIDSVKYIFSIIKDLKQASSTVILITHSPEVLKRADHAFLICAGTLVDKGDV
                     SRMFDYFSGKCVPCPHKGQPDTDRLVPMFAAN"
     gene            4290..5234
                     /locus_tag="PK105_07400"
     CDS             4290..5234
                     /locus_tag="PK105_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOU61577.1"
                     /translation="MAEQSIIDKDILIAELLASINQHKFAPDVAHIEIHGNEVLNRNL
                     VDGLIVESQSLEDGVSVKIRVKRGVTIENPVYFCFGLIPENGVQRIFINTLIEEGACA
                     QFIANCTFPNAINIQHLMNAEIELEKGASLSYFERHVHGPNGGVQIVPVTKVRLSEGA
                     RFSTEFELIKGAAGVIDLDYAAEVGKDATVDMKTRIFGRLEDSIRIKESANLLGESST
                     GVLTSHIAIKDKASAIIENEIIADAPYARGHVDCKEIVQDGARARAIPIVQVNNHLAH
                     VTHEAAIGSVDSKQLETLLSRGLTEDEATDLIIEGLLS"
     gene            complement(5410..>6114)
                     /locus_tag="PK105_07405"
     CDS             complement(5410..>6114)
                     /locus_tag="PK105_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61578.1"
                     /translation="FKIRDVLEEPLIIHKIKASVEERNTMIVRALEEVKLTPPDDFMG
                     RWPHMLSGGQRQRVATARTLILNPMMLVADEPVSMIDLSTRAEVLHMMKEVQKKLGLS
                     YLYITHDLSTARYFTDRIAVMYLGRIVEIGLADDIIDNPLHPYTQALIEAVPEPESGK
                     VKIIKELPIYGEIPSPADIPNGCRFHTRCPYAIEACKTEPEPELMLDGEEHYHACKRA
                     HEVKEEKQKKSRVERS"
BASE COUNT         1686 a         1388 c         1441 g         1599 t
ORIGIN      
        1 tacaaactac ctcatggcca ccctccaccc tcttatttcc atgactcagc tttcaaacaa
       61 ctgggcaaat ggcttggcgt catcaaagcg tattgctgag gtgctagatg ccgagccaga
      121 ggttgtggag ctgccaaatg cctctccgct tccatcaagc attcacggag aggttgaatt
      181 cgatcatgta tatttccatt acaatggaga tacagccctt aaggtgctgg aggatatatg
      241 tacaagagcc gaagcaagaa aggttaccgc cgtactgggt gcgacaggtt cgggaaaatc
      301 cagccttgtc aacctgattc cgcgtttcta tgatgctcgc tcaggaaagg tcaatatcga
      361 ttccaatgac gttagactcc taaggatttc atcgattctc gaacacatag ccatagttcc
      421 tcaggaaact gtgctctttt cgggcacagt gcgcgataac attcgatatg gaaagcccga
      481 tgcaagcgat gaagaggtcc ttgccgccgc agttgcggct catgccaacg actttatcat
      541 ggatatgcca gagcattacg agacgcacat cgaagaacgt ggggctaatc tctctggcgg
      601 acaaaaacag cgtattgcca tagctcgtgc ccttattacc aagccttcga ttctcatttt
      661 ggatgacgca acaagcgcta tagatgtgga aacagagacc aaaattcacg ctgccatagc
      721 gaaggccact atgggaataa ccctcatcat ggtggctcaa cgcatcagta cagtgcttaa
      781 cgcagacaag ataatcgtgc tcgatgaagg acgaatcgtt gcagaaggca ctcatagaga
      841 gcttctaaag aaaagcccaa tctacaaaga aatatacgat tcccagttgg gtgcgggggt
      901 gcgtcatggc ttatgagaca agtccctcga ttcaggatgt gcgcaccagc tttcaaaggc
      961 gaggaggcag gcctacaaga tatgagagag cctcggatcc tcgcaaagcg atgaaaaggc
     1021 tcttcggata tctaatgcca ttcaggcaac ctctgctttt gattgtgttc tttgtgcttc
     1081 tgcaatctct ccttggatta gtcggtcctt atttgctcgg cagcgcaata gacaagagca
     1141 tcggcggcaa agacataaat ggattagctc gaaatgctgt tcttatgctc atcgcttatt
     1201 ttctgagcaa tagctttaat ttgatcgcaa atcgaaaaat ggcgatcata tcccaaaatg
     1261 cccttaaaaa cctacgccgc gatttgtttg tgcatatcca gtggctccca atgcgctttt
     1321 tcgatacgaa ccccgctggc gggctcatga gcaggctaac gaatgacatc gatgccatca
     1381 atcaggctgt atcacaaaat atcatttcac tcgtcgcggg tgttattacg atgtccggca
     1441 ttctggtgag tatgttcgtt ctcaaccatt ggctagcgct tacaagcctc atcgtcatcc
     1501 cgataatgta ttttttcagc aattttgtgg caaaatatac tcgtaaggga ttcagggaat
     1561 tgcagaaaga gcttggtgat ctcaacgcta tagccgagga aaccatttcc ggctttaagg
     1621 tcataaaagc cttccgccga aatgactccg caatcgaaac cttcaaaaga aaaaatgaag
     1681 ctgtatataa agcagctata tatgcaaatt catatgcatt gcttctaatg cctcttacag
     1741 gtgtgatagg tagtttcttt gttatattgc ttgctagcct tggtggctgg ttggcgctca
     1801 aggatcttgt gagcattggc atgattgcta cattcataaa ctatgcacag aattttactc
     1861 agcctctaag gcaaatatca aatctataca actcgataca agccgctcta gctggagcgg
     1921 agcgagtctt cgagataata gatatggatc cagagatagc agaggaacca agactggaag
     1981 aagccaaaac aaatcccgag actgcaataa gagtcagcga agattctata atcggttctt
     2041 ccacggctcc aatggtcttt agtctcggac aatttcaggg caatgtcagc ttcaagaatg
     2101 tggtattcgg ctatttagcc ggcaaacccg taatcaaaaa tttcgatgta gatatttctg
     2161 cagggctgac ggtagcctta gtggggccca caggagccgg caagactacg attactaacc
     2221 tactgatgcg cttctatgag atcgatgaag ggcagatact attcgatggc gtagatatcc
     2281 gaactattcc gaaggaagca gttcggcgag ccataggttt ggttttgcag gatacttttc
     2341 tctttgctga ttcggtactg gagaatattc gctttggccg attggatgcg acagacgagg
     2401 aatgtatcga agctgctaag atggcggaag tcgatcactt tatacgacag cttcccgaag
     2461 gttatcaaac aaatctttcg gagcgtgcaa gcaatttaag ccagggccag cgtcagcttt
     2521 tatccattgc gcgggcaatt ctagcggatc cggcaatact tatattggac gaagccacaa
     2581 gcagcgtcga tacacgcaca gaaattcgca tccaaagggc tctcttgagg ctcatgcacg
     2641 gaagaacaag catagttatc gcccataggc tcagcactat tcgcgatgcc gacaagatca
     2701 ttgtaataga tgatggcaaa attgtggaaa taggagacca tcaagcgctc cttgcaaagc
     2761 gcggctttta tcatcgtctt tacatgagtc aattcaaggg aaatgaaata taatcgaatc
     2821 atatagatat atcaaaacac tggaggaaaa agcatgaatc ggcaggatat accactgaaa
     2881 gattttcact acgatgtagc aagactacgc gaccattggt ttgcgttatt tgcaggtgat
     2941 tttaatgctc gtgtcttcaa cgcgatgaca atttcctggg gcagttatgg gcatgtatgg
     3001 gataagatgg tgtttcaggt tatggtacgt ccctcaagat atacctatga tttcatgaac
     3061 cgatacgaca gctttactct aaacgctttt actccagagt acaaacaagc tctatcgatt
     3121 cttggttcga attccggccg aaacctcgat aagatagcac tggctggtct tactcctatg
     3181 gcttcccgca aggttgatgc cccaagtttc gaggaagctc agcttgtaat agaatgccga
     3241 aaaatctatt ggcatgactt aaatgaagct aatttcctgg atagtgatat agaagagcat
     3301 taccccagaa aggactatca tcggatctat ctaggcgaga ttgtcaatgt tagtcagcag
     3361 ataatcaaac catagggttt gccgctctaa atattcggtt tgctgccaca tacttgattt
     3421 tctttttgga aaggagtatt tttctcaaag gaaaatccta cattaatgat atgaatttta
     3481 ttcagagcat aatgcagctc agccaaagac acgggacggc aaaattatga atcttctcga
     3541 aatcaagaat ataacgcttc tcaaagaatc aagacccatt ttgaacaatc taagcatgga
     3601 gatctggaat ggacatgtcc atgcgatcat tggtcctaat ggagcaggta agtccactct
     3661 ggctaatacc ataatgggac tatccggtta tcgcgatttt caaggtgatg tcatttttaa
     3721 tggtgagtct cttaaggatc tttcgataga tgagcgggca cgaagaggaa ttacattgct
     3781 tttccaagaa cctgcccgct tcgaagggct gtccgtgagc cgattcatac ttgcaggagc
     3841 caaagagaaa tctcagaagg tcgtcgagga tgcgcttcga caggtaggac ttgtaccaga
     3901 aaactatatc aatcgcgccg tggataagac actctcggga ggcgaacgaa agcgcatcga
     3961 actagcttcg atatatgcga tgaagcctcg ccttgtgctt atggatgagc cagattccgg
     4021 tgtagatata gattctgtaa agtatatctt tagcattata aaagatctaa aacaggcaag
     4081 ttccactgtc atcctaatca cccatagccc tgaggtcctg aagcgcgcag atcatgcttt
     4141 tctcatctgt gcaggaactc ttgtcgacaa gggcgacgta tctagaatgt tcgactattt
     4201 cagtggcaag tgtgtgcctt gtccacataa aggccagccc gacacggata ggctagttcc
     4261 tatgtttgcg gcgaattgag aggaaaacca tggcagagca atcaatcata gataaagaca
     4321 ttcttatcgc agagctgctg gcatcgatca atcaacataa attcgctcca gatgtggcac
     4381 atatagaaat ccatggcaat gaggtcttga atcgaaatct cgtcgatggc cttattgtag
     4441 aatctcaatc cctggaggat ggggtcagcg tcaaaattcg agtcaagcga ggtgttacga
     4501 tagagaatcc agtatacttt tgcttcggtc ttattcctga aaatggggta cagcgaatct
     4561 ttatcaatac cttaatcgag gaaggagcct gcgcccaatt cattgcaaac tgcacctttc
     4621 caaatgcgat caacattcaa catcttatga atgcagagat cgagctcgaa aagggcgctt
     4681 ctctatctta tttcgagcgg catgtccatg ggcctaatgg cggggtccaa atcgtaccag
     4741 ttacaaaagt gaggctaagc gaaggcgcca gattttccac cgagttcgag cttatcaagg
     4801 gagctgcggg tgttatcgac ctcgattatg ccgcagaagt gggaaaagat gccacggtgg
     4861 acatgaagac tcgtattttc ggtcggctgg aagacagtat ccgaataaag gaatccgcca
     4921 atcttcttgg cgaatcttcg acaggtgtat tgacaagtca tatcgcaatc aaagataagg
     4981 cttccgcaat catcgaaaac gaaatcatcg cggatgcacc ttatgcccgg ggtcatgtcg
     5041 actgcaagga aatcgtccag gatggcgcgc gcgcccgtgc gattcctatc gtgcaggtga
     5101 acaaccatct tgcccatgtc acgcatgaag cggctattgg ctcggttgac tcaaaacagc
     5161 tcgaaacttt gttgagccgc ggtctcacag aagacgaggc aacggatttg ataatagaag
     5221 ggcttttgag ctaattctgc atggctccgg actcgctctt ggactttctg tacatctgct
     5281 tttatccaag gtttaagccg ctcgattcca aagcgatttc ttaattcagc acccagaaat
     5341 tgaaatggtg ttatataaca tgatcggcta tccatgattc tacaatggca ctacagcctt
     5401 tgttttatct catgatcgct ctaccctgct tttcttttgc ttctcttcct tgacctcatg
     5461 agctcgttta caagcgtgat aatgctcctc tccgtccaac atcagctcgg gctcgggttc
     5521 cgtcttacaa gcttcgatgg catagggaca gcgcgtatga aaacgacagc cgttaggaat
     5581 gtctgctggg ctcgggatct ctccatagat tggaagctct ttaattatct tcaccttgcc
     5641 tgattcaggt tccggaacag cctcgataag agcttgagta tagggatgca aggggttatc
     5701 gatgatatca tcggcaaggc caatttccac tattcgaccc aggtacataa ccgcaatgcg
     5761 atcggtaaaa tagcgtgccg tcgaaagatc gtgggttata tagaggtaag acaggccaag
     5821 ttttttttgt acctccttca tcatgtgaag gacctcggct cgcgtggaaa ggtctatcat
     5881 cgagaccggc tcgtctgcaa ccagcatcat tgggttgagt atgagcgttc gtgccgttgc
     5941 gactcgctgg cgctgtcctc cggaaagcat gtgcggccat cgacccataa aatcatcggg
     6001 cggcgtcagc ttgacctctt caagggctct gacaatcatg gtattgcgct cttcgacgga
     6061 cgccttgatc ttatggatga tgagaggctc ctccagcaca tccctgattt tgaa
//
LOCUS       DAOOWY010000106         6078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_13102_length_6078_cov_10.194587, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000106 DAOOWY010000000
VERSION     DAOOWY010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13102_length_6078_cov_10.194587"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PK105_07410"
     CDS             <1..555
                     /locus_tag="PK105_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61579.1"
                     /translation="LFSCAPKLAQKDLDAANALFAEAQEAKADVYAPDEFMAAQDAKA
                     ALEAELAAQDAKTSGKSYKQANDLIKAFADASTKAKEAASTNKEQVKNEVAQLIVDVD
                     AQYTTVQALAQAAAKDTKKAAKAKLNVKDINAKVAAMNEEIANAKAANDAEDFAAARD
                     KLNAIKATLDELQSTLESAGFAAQ"
     gene            743..1756
                     /locus_tag="PK105_07415"
     CDS             743..1756
                     /locus_tag="PK105_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61580.1"
                     /translation="MPRFAGSYRVLADSPPDMISFEECLVRYWRGNDAILLFYVNPPS
                     VIIGRNQNYWREVAPSCTIPVFRRASGGGAVYHDLGNLNWSMIVPRELHSKEEELCLM
                     AKAISKLGVYTHIGARGEIFVSMRDGETEAKISGTARYFGTHNVLHHGTLLISSNLKT
                     LHACLGGIKVLEDNSIPSVPATPVNLVELKPSLTVCEIISMLSLELAGRDPCEISLSE
                     FWNSRMPHLEIALRDLGVLKGYLASTIDYEDFETFRAHFSSIEWIKDRSPPFSILLSS
                     DNRRIVLKIEGGRVSRILPFEPDDFGAANLAALLQTRFIGLQFDFDTVELIGRVADDE
                     FAW"
     gene            1935..3356
                     /gene="lpdA"
                     /locus_tag="PK105_07420"
     CDS             1935..3356
                     /gene="lpdA"
                     /locus_tag="PK105_07420"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOU61581.1"
                     /translation="MKEREFDFIVIGAGPGGYVAAIRAAQLGLKTAVVEKNLVGGVCL
                     NVGCIPSKSLIHNARLFAEGGKMLQKAGAKIDMSGFDYEPIWKTSRLVAEKLSKGVNF
                     LLKKNKVELIQGTAKLTGASTVEVEGADAAIELRSKAILLATGSRPRIIPGFELDEKR
                     ILSSTGILMKNTLPKRLFILGAGPIGMEFAYIMNAFGVDVTVVELLPRALPLEDEEVS
                     RIIEKEFRNRGVTIYTGANAKSAEIRNNEVVVHVVDEQTKAMDIYADAALVSVGRTPN
                     TDNLGLEQLGIKIDRGYILTGDYHETSCSGIFAVGDITTYPQLAHAASKAGEIVAERV
                     AHLLKGTPNPQERSLDRLHVPSAVYCDPQVASFGLSEASAKEKCIDHQVARFPYRGNG
                     RAVAEDAPEGQVKLVFDRSNGAILGASIIGEGAADLIHEPLLAANAGLRIDDIAALVH
                     AHPTLSETIMEASKVALDRAIHI"
     gene            3436..3786
                     /locus_tag="PK105_07425"
     CDS             3436..3786
                     /locus_tag="PK105_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU61582.1"
                     /translation="MFGKHSSAGFHETSPGNRIKTLCYGEHTLLVEVHLDKDADLLRH
                     KHPYEQTGYLVSGKIVMHIEEKAVELKPGDSWCIPMNAEHKVDVLEDSVVIEVFSPTR
                     EEYIKYKNDNDILQ"
     gene            complement(3840..5300)
                     /locus_tag="PK105_07430"
     CDS             complement(3840..5300)
                     /locus_tag="PK105_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU61583.1"
                     /translation="MNSISQTLGKSAMNIALNYLNKDPEKNIPKIVDLFMKFTFREDM
                     KRQLLSAKEVMNDPNNPYRGLIMRAFYELDPKIRKRFLTNFVFNSGIIGQDKTAVLKV
                     KYNCNIPWTILMDPTSACNMHCKGCWAAEYDKTDSMDYELLDRIIREGKELGIYFYIY
                     SGGEPMLRKDDLIKLAKVHDDCVFLAFTNGTLIDDACAKELAESGNFGLAFSIEGFEN
                     ATDFRRGKGTYQKVVQGMKNMKKYGAPFGFSACYHSKNAEAVGSEEFLDFMIEQGCMF
                     GWIFTYMPLGKDADTSLLATPEQREYMFHWVRKMRDTRPLFLIDFWNDGQYVNGCIAG
                     GRSYLHINAAGDVEPCAFIHYSNVNIKNTSLLDALRSPLFKQYAENQPFNENHLRPCP
                     LLDNSGKLAEMVKAAGAYSTQPIDREDVDNLTAKCVQAAERWAPVAEKLWAGEMPHYL
                     ESKKKSKELQDKNLEVIKKKLKKGKKTKDSNLEAAS"
     gene            complement(5401..5976)
                     /locus_tag="PK105_07435"
     CDS             complement(5401..5976)
                     /locus_tag="PK105_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU61584.1"
                     /translation="MPRIVDHDKRKREIARSALMLFSKQGYHESSFSDIASACRLSRS
                     NIYNYFKNKDEIFHYAVVELLNSITQNIELIAHQDSLTLAQKLQRIYQLFTNDFGDGK
                     NASIILDLSLRLKRKDTKLVATLDNSAVNLRKNIENLFVSENANLPYVQIAVVTTLFF
                     SLIESSITHSLFTEKAFIQQNIAVILQMLGI"
BASE COUNT         1656 a         1306 c         1421 g         1695 t
ORIGIN      
        1 ttgttctcat gcgcgccgaa gcttgctcag aaggatctag atgcagccaa tgcactattc
       61 gccgaagctc aggaagctaa agccgatgtc tatgctccag atgaatttat ggctgcccag
      121 gatgccaaag ccgcactcga agccgagctt gcagctcagg atgccaagac ctctggaaag
      181 tcttataagc aggcaaatga ccttataaaa gcttttgcag atgcaagtac aaaggccaaa
      241 gaagctgcgt ctacaaacaa ggaacaggta aagaacgaag ttgcacagct aattgtggat
      301 gttgatgctc aatacaccac tgttcaggct cttgctcaag ccgcggcgaa agatacaaag
      361 aaggcggcaa aggcaaagct caacgtaaaa gatatcaacg caaaagtcgc tgcgatgaat
      421 gaagagattg ccaatgcaaa agcggcaaat gatgctgaag atttcgcggc tgctcgtgac
      481 aagcttaatg cgatcaaagc cactcttgat gaacttcagt caacccttga atctgccgga
      541 tttgccgctc aataataata aagtatatca agtagtggca cgagctatac tatagaataa
      601 agaagtcggc aacgctttgc tattcaagtc atagctcctg catttcgata aaagaccgct
      661 cctaaagggc ggtcttttgg tttaatggta tcagagacta aaatgcgttg gaattggcaa
      721 aaatttctta tatattggaa acatgcctag atttgctgga tcttatagag tcctggcgga
      781 cagcccgcct gatatgattt ctttcgaaga atgcctcgta agatattgga gaggaaatga
      841 tgctattctc cttttctatg ttaatccccc gagcgtgata ataggaagga atcaaaatta
      901 ctggcgggaa gttgcacctt cttgtacgat cccggttttt cgacgtgcct cgggaggagg
      961 cgcagtatac catgatctcg gcaatttgaa ttggtccatg attgttccga gggaacttca
     1021 ttcgaaggaa gaagagctgt gcctgatggc taaagcgatt tccaagctcg gagtatatac
     1081 acacatagga gcgagaggcg agatctttgt gtcaatgcgc gatggtgaaa cagaagcaaa
     1141 gatctctggt acagctaggt attttggtac tcacaatgtg ttacaccatg gaacattgct
     1201 tatatcttcc aatctgaaaa ctcttcatgc ctgtctagga ggaatcaagg tattagagga
     1261 taattcgatc ccatcggtgc cagcaactcc ggtcaatttg gttgaattaa agccttcgct
     1321 cacggtttgc gagataattt caatgctttc acttgaactt gcaggaaggg atccctgtga
     1381 gataagcctt tccgaatttt ggaatagccg catgccccat ctcgagattg cattgagaga
     1441 tctcggagta ttgaagggat atctagcatc aaccatagac tatgaggatt ttgagacatt
     1501 cagggcacat tttagttcta tagaatggat taaggatcga agcccccctt tttccatact
     1561 cctttcaagc gataacagaa gaatagtcct gaaaatagaa gggggcagag tgtccaggat
     1621 actgcccttc gaaccggatg attttggagc cgcaaatctt gccgcgctcc ttcagacgag
     1681 gtttattggt ctgcaattcg atttcgatac tgtggagttg attggcaggg ttgcggatga
     1741 tgaattcgca tggtgagcca aagagctcat gaaaattccg cattctaggc cagaattctc
     1801 ttcagcctct ccttgacctc aagccatgac cgttttagca ttggtagcaa gattggtccg
     1861 ccttatttgc tgaccttacg aaaatacaaa gatagcccat aaaggcaatc gacggaattc
     1921 caggagcaat aatcatgaaa gagagagaat tcgactttat tgtaatcggt gcgggtccag
     1981 gtggttatgt tgctgcgatt cgtgcggcgc agttaggcct taaaactgcg gttgtggaaa
     2041 aaaatcttgt aggaggagtc tgtctcaatg tgggttgcat tccctcgaaa tcgctgatcc
     2101 acaacgctcg gctttttgcc gaaggtggaa agatgttgca aaaagccggt gcaaagatag
     2161 atatgtctgg ctttgattat gagcccatat ggaagacttc tcgcctagtt gccgaaaagc
     2221 tctctaaggg agtcaatttt ctattaaaga aaaataaagt agaactcatt caaggaacgg
     2281 ccaagctcac tggagcaagc acggtcgagg tagaaggagc tgacgccgct atagagctta
     2341 ggtctaaagc aatacttctt gccaccggtt caagaccgcg aatcatcccg ggtttcgaat
     2401 tagatgaaaa gcgcatcttg tcttctacgg gcatattgat gaagaatact cttcccaaac
     2461 gcctctttat tctgggcgct ggcccgattg gaatggaatt tgcttatata atgaatgctt
     2521 ttggcgtaga cgtgactgtt gtggagctct tgccaagggc actaccctta gaagatgaag
     2581 aagtctcaag aattattgaa aaagaattcc gtaatcgagg tgtgactatt tacactggtg
     2641 caaatgctaa aagtgcggaa atcaggaata atgaggtggt cgttcacgta gtggatgaac
     2701 agaccaaggc catggatatc tatgcggacg cggcccttgt ttcggttggg cgtacgccca
     2761 ataccgataa tcttgggctc gagcagctag gaatcaagat agaccggggc tacattctaa
     2821 cgggcgatta tcatgaaacc tcttgctcgg gtatatttgc ggtaggcgat attacaacct
     2881 atcctcagct ggcgcatgcg gcttccaagg ctggagagat tgttgccgag agagttgcgc
     2941 atcttttaaa gggaactcca aatccgcaag agcgcagtct tgatagactt catgtgccga
     3001 gtgctgtcta ttgcgatcct caagtggctt cttttggtct gtctgaagct agtgcaaagg
     3061 aaaagtgcat tgatcatcaa gtagcaaggt ttccttatcg gggcaatggg cgcgcggtgg
     3121 cagaagatgc tccggaaggc caggtgaagc ttgttttcga tcgatcgaac ggagcgattc
     3181 tcggtgcctc gattataggc gaaggcgcgg ctgacctgat ccatgagcct ctcctggccg
     3241 cgaatgctgg gctcaggatc gatgatatcg cggctcttgt gcatgcccat ccaacactgt
     3301 cggagacgat tatggaagct tcgaaagttg cgctggatag ggctatccat atctagagca
     3361 aagggatttc tatgcttatt gtatatgctt attattgaaa attatcatac gagaaattac
     3421 tatgggagga acatcatgtt tggaaaacat tcaagcgctg gcttccacga gacatcgcct
     3481 ggaaatcgta tcaagactct atgttatggc gaacatactc tactcgtcga agtccatctg
     3541 gataaagatg ccgatcttct aaggcataaa cacccctatg agcagacggg ctatcttgtt
     3601 tcaggcaaga tagttatgca catcgaagaa aaagcagtgg agctcaagcc tggcgatagc
     3661 tggtgcattc ctatgaatgc cgagcacaag gtggatgttc tcgaggatag tgtcgtcatc
     3721 gaggttttta gcccaacgcg agaagaatat ataaaataca agaatgataa tgatattcta
     3781 caatgagcat tagtcattct tttaatagta cctggcttac gctttacggg aagacaggct
     3841 caagaagccg cttccagatt tgaatccttt gttttcttgc ccttcttgag cttcttcttg
     3901 attacttcga gattcttatc ttgtaattct ttactcttct ttttggattc gagatagtgg
     3961 ggcatctcac cagcccagag cttttcagcc acaggcgccc atctttcggc agcttgcaca
     4021 cactttgccg tcagattatc aacatcttcc ctatctatgg gctgcgtcga atatgctcca
     4081 gcagccttta ccatctccgc aagtttccca ctattgtcca aaagagggca tggcctcagg
     4141 tgattttcgt tgaacggttg attctcggca tattgcttga acaagggtga gcgcagtgca
     4201 tccaggagag atgtattctt gatgtttacg ttcgaatagt gaataaacgc gcaaggttcg
     4261 acatctccag cagcgttgat atggaggtag cttcttccac ccgcaatgca tccatttaca
     4321 tattggccat cgttccagaa atcgatgagg aagagcggcc ttgtatcgcg catcttcctg
     4381 acccaatgga acatgtactc gcgctgttct ggagtggcta agagactagt atctgcatct
     4441 ttccccaggg gcatatatgt gaatatccag ccaaacatgc agccttgttc gatcataaaa
     4501 tcgagaaatt cttcgctacc aaccgcttct gcattcttcg aatgatagca tgcgctaaat
     4561 ccaaagggag cgccgtattt tttcatgttc ttcatgcctt ggacaacttt ctgataggta
     4621 ccttttcctc gtctgaaatc agtagcgttt tcaaaacctt cgatgctgaa tgccagtccg
     4681 aagttcccag attcagcaag ttcctttgcg caagcatcat ctatcagggt accgttcgta
     4741 aatgccaaga atacgcagtc atcatgaacc tttgcaagtt ttattaggtc atcctttcta
     4801 agcatcggct ctccgccgga gtagatatag aagtagattc caagctcttt tccttctctg
     4861 atgattcgat cgagaagctc ataatccatg gaatcggttt tgtcatactc tgcggcccag
     4921 caacccttgc agtgcatatt acatgcactt gtgggatcca taaggatagt ccatgggata
     4981 ttgcaattgt atttaacctt cagaacagca gtcttatctt ggccgattat tccggaattg
     5041 aagacaaaat tggttagaaa gcgcttgcga atcttcggat cgagttcgta gaatgccctc
     5101 attatgagac ctcgataggg attgttggga tcattcatca cctcttttgc actcaatagc
     5161 tggcgcttca tatcttcacg gaaggtgaat ttcatgaata gatctacgat ttttgggata
     5221 ttcttttcag gatccttgtt taggtaatta agagcgatgt tcatcgcact tttccccaat
     5281 gtttgtgaga tggaattcat atttccctcc tctttctttc tattgtcagc aagagcactc
     5341 tgtcaatata accacaagga atctccatgt tcgctttcaa caaagcacct tttcctacaa
     5401 tcatattccc agcatttgca ggatcactgc tatattctgc tgaatgaatg ctttttcagt
     5461 gaaaagagaa tgcgtaatgc tgctctctat aagagaaaaa aagagagtcg taactactgc
     5521 aatctggaca taaggcagat ttgcattctc gctaacaaag agattctcga tgtttttgcg
     5581 aagatttaca gcagaattat caagggtggc aacaagcttg gtatcttttc gcttgagtct
     5641 cagcgaaaga tcgaggatga tcgaggcgtt cttcccatcc ccgaagtcgt ttgtaaaaag
     5701 ctggtaaatt cgctggagct tctgagctaa tgtcaagcta tcctgatggg ctatgagctc
     5761 gatattttga gtaatcgagt tgagcaattc aactaccgca tagtgaaaga tctcatcctt
     5821 attcttgaag taattatata tattagagcg gcttagtctg caggccgatg ctatatcgct
     5881 aaagctagat tcatgatagc cttgcttaga aaacagcata agtgcagacc gggcgatttc
     5941 tcgttttctt ttatcatgat ccacgatcct tggcataagc tttgtctcct aaattatact
     6001 ttggtgcaat tatttattct gacagctatg tcaaatataa ttaggccaaa gacttctgtc
     6061 aacgattaat gacgattc
//
LOCUS       DAOOWY010000107         6074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_13118_length_6074_cov_6.000000, whole genome shotgun sequence.
ACCESSION   DAOOWY010000107 DAOOWY010000000
VERSION     DAOOWY010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13118_length_6074_cov_6.000000"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PK105_07440"
     CDS             <1..484
                     /locus_tag="PK105_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOU61585.1"
                     /translation="NGAIKRFIVMAGCDGRQPKRSYYTEVAQKLPKDTVILTAGCAKY
                     RYNKLDLGDIGGIPRVLDAGQCNDSYSLALIALKLKEVFGLDDVNKLPLSFDIAWYEQ
                     KAVLVLLALLYLGFKNIRLGPTIPGFLSPRTVELLVENFGIKGIASPDEDIASMMVGA
                     "
     gene            508..1091
                     /locus_tag="PK105_07445"
                     /pseudo
     CDS             508..1091
                     /locus_tag="PK105_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
     gene            complement(1176..2537)
                     /locus_tag="PK105_07450"
     CDS             complement(1176..2537)
                     /locus_tag="PK105_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU61586.1"
                     /translation="MAQKKIKTRGELSRKTVLLWALIALVLSALTNGYHYSLEGAKGL
                     YVWHPLIILKILLYMGLGPITIAILSLPLEKLAKKWPNIALRWASFVSSLLVSIVSIT
                     LFAFLIIVPRASSLEPARLKLIDPSKGVFPQESFEAPSFSRLENDDIDSTDSTGSGIH
                     AGSDSSADSAIHSHSMLPASIDTPLLKLSFSSDPHWGAETANAGARVRILEELADRRP
                     DAFFMLGDAVETGNNSTQWNFALSDLEAITPDIALRPIMGNHDALFGGQYLYKKAFWP
                     KDFVSDSGSPYYWSISSGPATIVAVDLPWGTEMWDARQKAWLEKTLAAAEPHKPLIVI
                     SHSYFYASGYEDPDFGSPWYDHYQNIPALVPLFEKYGVDLVVSGHNHYQELLSHNGVT
                     YAIVGSMGGIPDPEPSYRSPCSQWIEVGGYGWLDVEVLPGQLTLTFRSETGQERQSVT
                     IAY"
     gene            2708..4225
                     /locus_tag="PK105_07455"
     CDS             2708..4225
                     /locus_tag="PK105_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOU61587.1"
                     /translation="MTRRIGKIAVLIMAIFALTFTGIVSAQDQLPGNVTITFAHTNDM
                     HGRIVEGADVLGFPKIYSAVEQLKASNPNTLLIDAGDTFHGLPVVNIDRGETAIKLMN
                     ELGYVYMTTGNHDYNYGFDRLMELSKMAEFKILAANVYKDGERVFPAYDIREMGGVRV
                     AFFGLATPETAYKADPKGIEGVTFGDPIVEAKLIVAELAGKYDLLVCISHVGIDDSSD
                     PTSITVAKFVPQIDVIIDGHSHSTLADIEKKNTTSTLITSTGSYGAGLGVVDIVVGPD
                     RKVISKTARTITPKNSPDLKANPSIAKVLDDLVKAQDAVLSQVVGRTEIKLEGTREIV
                     RTQQSNLGTLIANSMLYATGADFALMNGGGIRDSIPVGDITMKQIYTVQPFGNFIQSG
                     KVLGKEIDAILENGVGKLPAPDGRFPHVANLTYTLDASKPAGDRVSNIMIGGEPVDPE
                     KEYTIATLNFIFNGGDDYRMLIGKAQNDYPTDAEVFMSYIKYLGTITNDNIVFKK"
     gene            complement(4409..5185)
                     /locus_tag="PK105_07460"
     CDS             complement(4409..5185)
                     /locus_tag="PK105_07460"
                     /inference="COORDINATES: protein motif:HMM:NF022789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HOU61588.1"
                     /translation="MEIALSFVLAGVWISGATLLGEKLGSKRAGLITNLPSSILLSLI
                     FMALTMGPEYAAATTKGIPVGMTIDTLFTFVFILMARKGLSKALTISLIVWAITAYVF
                     IKLIPPLGMAGAIGLYVLVTALCFILAEGVLHIRSVPHKETTFSWKQILLRALFAGTV
                     VAGAVTIAQFAPPYMTGILATFPAVLSSTLVIFTLSQGADFARATGKILILSSSNIII
                     YAWIAGLTFPSLGPWIGTILSFATSVAYVALLGKLTAKIK"
     gene            complement(5216..>6074)
                     /locus_tag="PK105_07465"
     CDS             complement(5216..>6074)
                     /locus_tag="PK105_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61589.1"
                     /translation="GQYTKDRIVDKNGSRIVNGGAYYYGAAAAAAMGLKVAAITRLAE
                     EDFPSFSPLIERGVRVKAIATPKSTCLRLFYPSNNPDERILSVTSIAQPFEPSDIESV
                     SAPIWSIGASIRGEVSLEVLKAIKAKNARIGLDAQGFVRVVRDGQLAYDSDWPEKREV
                     LSLVDVFKADVVEAEILTGTRDLYEAARILARFDVPEFVLTHGGGVVVYAEGSFFEAP
                     FLPKTMEGRSGRGDTCLSSYLSARIFMPPKAATLWAAALTSLKMETLGPFLGTRDDVE
                     QAIRERYSI"
BASE COUNT         1660 a         1397 c         1474 g         1543 t
ORIGIN      
        1 aaatggcgcg ataaagcgat tcattgtcat ggcaggttgc gatggccgtc agcccaagcg
       61 ctcatattat accgaagttg cccaaaaact ccctaaggat accgtaattc tgaccgcggg
      121 ttgcgctaaa taccggtata acaagctaga cctgggcgat attggtggca ttcctagagt
      181 gctcgatgca ggccaatgca atgattctta ttcgctcgcg cttatcgcgt taaagctcaa
      241 ggaagtattt gggctcgacg atgtgaacaa actgccactc tccttcgata ttgcatggta
      301 tgaacaaaaa gccgtgctgg ttcttcttgc tctcttatat ttgggattca agaatattcg
      361 tcttggtccc acaatcccag gattcctttc tccgcgaaca gtagagcttc tagttgaaaa
      421 tttcggaata aaagggattg cctcgcctga cgaggatatt gcttcgatga tggtaggagc
      481 atagagtagt ctagaggagg atgcacaatg gacgaattgc acacaagact ataccaagct
      541 agaggcttaa gaaccgtttc cgcgctccaa aagaacgctt tcgatgctct atatgtggcc
      601 accgctgccg aagctgcaga aaaggtactt gcatttgtgt acgaaggcgc cagggttggt
      661 ttcggaggct caatgacaat caaatcgatg aatatccaag aattggcagc aaaaaaattc
      721 ttcgatctca gctcacctgc gatgtcttta tctcgagcgc aaatgccatc accatggaag
      781 gctttatttt caacgtggac ggaaatggga accgcgttgc tgcacttagc tttggcccga
      841 agaaaaatgt cattgtggcg ggaatgaaca aggttgtcgc aaacctcgat gaggcttacg
      901 agagactaag aacctatgct tcgcctctca ataatattcg gctcgaaaaa cccaacccat
      961 gcgtaacgca gggctattgt gccgattgtg ttcttcctac acggatatgc agaatttatc
     1021 aggtattaag acgcaagcct agcttgagcg atttcaccgt gattctggtt ggggaagatc
     1081 tgggattttg aagggcttta ttggtaacag atgattcttg ctgatcgatg tggagattgg
     1141 cttcaaaatc gataatttgt ctagataatg gtgattcaat atgcgatagt cacgctctgt
     1201 ctctcttggc cagtttcgct gcggaaggtg agcgtcaatt ggccggggag aacctcgaca
     1261 tcgagccagc catagcctcc tacttcgatc cattgagagc agggtgatcg atagcttggt
     1321 tccggatcgg gaattcctcc catggatccg actattgcat aggttactcc attatgcgaa
     1381 agcaactctt gataatggtt atggcccgaa acgaccaaat cgacaccata tttctcgaaa
     1441 agtggtacga gcgcaggtat attttgataa tgatcatacc aaggcgagcc gaaatcgggg
     1501 tcttcataac cagaagcgta aaaataacta tgtgaaatca caataagagg cttgtgcggc
     1561 tcagcggccg caagtgtctt ttcgagccaa gccttttgac gagcatccca catctcggtt
     1621 ccccaaggga gatcaacggc aacgatcgta gcagggccag agcttatgct ccagtaatat
     1681 ggagagcctg aatcacttac gaaatctttt ggccagaaag ccttcttata tagatattga
     1741 ccgccgaaaa gtgcgtcatg attacccata ataggacgca aggcaatatc cggcgttatg
     1801 gcttcgaggt ccgacagcgc aaaattccat tgagttgaat tgttgccggt ctccacagca
     1861 tcgccaagca taaagaatgc atctggcctt cggtctgcga gctcttctaa gatcctaaca
     1921 cgtgcgcctg catttgctgt ttccgctccc caatgcgggt cagaagagaa agacagtttt
     1981 agcagtggcg tatcaatgga agcagggagc atggaatgag aatgaatagc cgaatcggcg
     2041 gaactatcgg aaccggcatg aataccggaa ccagtcgaat cggtagaatc gatatcatca
     2101 ttctcgagtc ttgaaaaaga gggagcctcg aagctctcct gcggaaaaac ccctttagat
     2161 ggatctatga gcttgagcct tgcaggctca agcgaactgg ctctcggcac tataatcaag
     2221 aaggcaaaga gtgtaatcga tactatgctg accagcaaag agcttacaaa agatgcccat
     2281 ctaagtgcga tgtttggcca tttcttagca agtttttcga gcggtaaaga cagtatggca
     2341 atggtaattg gtccaagccc catatagagc aagattttta gtataataag ggggtgccat
     2401 acatagaggc cctttgctcc ttccaggcta taatgatagc catttgtcag agcggagagg
     2461 accaatgcta tcaaagccca gagcaatact gtcttgcgag agagttctcc tctcgttttt
     2521 atcttttttt gcgccattaa aataatatat cagcaatata ttccagcggt aagccattct
     2581 tggcgtcttg actggctttt ttgatgggag aatcgaacaa ttatctcttt ttgcttgtct
     2641 gtaggggaag agctgggtat aataatattg cttcttaaga acacagagat taattaggag
     2701 gttttcaatg acacgaagaa tcggaaaaat tgccgtgctc atcatggcca tttttgcgct
     2761 aacctttacc ggcatcgtgt cggcgcagga tcagctgccc ggcaacgtta cgatcacctt
     2821 tgcgcatacg aatgacatgc atgggcgaat tgtcgaaggt gctgatgtat tagggtttcc
     2881 gaagatctat tcagcggttg agcaactaaa ggcttctaac cccaatacgc ttctcatcga
     2941 tgcgggtgac acattccatg gtttacctgt cgtcaatatc gataggggtg aaacagccat
     3001 aaagctcatg aatgagcttg ggtatgtcta tatgacaact ggcaaccacg attacaacta
     3061 tggcttcgat cgcctgatgg aactctccaa gatggccgaa ttcaaaatcc ttgcggctaa
     3121 tgtctataag gatggagaga gggtatttcc tgcctatgat attcgcgaga tgggcggtgt
     3181 gcgcgtggct ttctttggac ttgcaactcc agagactgcc tacaaagccg atccgaaagg
     3241 catcgagggt gttacatttg gagatccaat cgtagaagca aaactgatag tagccgagct
     3301 tgcggggaaa tatgatctgt tggtctgcat atcgcatgtc ggtatagatg attcgagcga
     3361 tccaacttcg ataacagtag ccaagttcgt gcctcagatc gatgtgatca tcgatggaca
     3421 cagccatagt actcttgcag atatcgaaaa gaagaatacg acaagcactc tcataacaag
     3481 cactggctcc tatggggcag ggctgggcgt agtcgatatc gtagtcggtc cagatcgaaa
     3541 ggtaatctcg aagaccgctc gcacgattac tccaaagaat tcaccagatc taaaggcgaa
     3601 tccgtccatt gcaaaagtgc tggatgatct tgtcaaagca caggatgccg tgctctccca
     3661 ggttgtgggc agaaccgaaa ttaagctcga aggcacgcga gagattgtcc gaacgcaaca
     3721 gagcaatctt ggaaccctca tcgccaattc gatgctctat gccactggtg ccgattttgc
     3781 tttgatgaat ggtggtggta ttcgagattc cattcctgtt ggcgatatca cgatgaagca
     3841 gatatacacc gtccagccat tcggcaactt tatccagagc ggcaaagttc taggcaagga
     3901 gatcgatgct atcctagaga atggtgttgg caagctacca gctccggatg gtcgcttccc
     3961 gcatgtggcc aatctcacct atacgctcga tgcgtcgaaa ccagcaggtg atcgggtttc
     4021 gaatattatg ataggaggcg agccagtaga tcccgagaag gaatacacga tcgcgacgct
     4081 gaactttatc ttcaatggtg gagatgatta tagaatgctc ataggtaagg cccagaacga
     4141 ctatccaact gacgctgaag tctttatgtc ctatatcaaa tatcttggca ccataacaaa
     4201 cgacaatatt gtattcaaga aatagggaaa gcataaagag gcattttacg cattcctatc
     4261 agctgatatg atgtcttgat tggatttagg gaggctatcc gaagatttgg gtggcctccc
     4321 attttatgga tcctgatatc ctgattatct agaatacgat ggaagctggg ctttggcaaa
     4381 tccttctgag cagcgccttt aggctcactt atttgatctt tgcggtaagc ttaccaagca
     4441 gagcaacata ggctaccgag gttgcaaagg aaaggatagt tcctatccat ggtccaagcg
     4501 atggaaaggt caaacctgct atccatgcat atataatgat attgctcgaa gaaaggatga
     4561 gtatcttacc agtggctcgt gcaaagtccg ctccctggct caatgtgaaa ataacgaggg
     4621 tactagaaag cacagctgga aaagttgcta aaatgccagt catatagggc ggcgcaaatt
     4681 gcgctatcgt cacggctccg gccacgaccg ttccggcaaa aagagccctt aaaagaattt
     4741 gcttccagga aaaagtcgtt tccttgtgcg gaactgagcg gatatgcaaa accccctctg
     4801 caagaataaa gcataaggca gtcactagga catatagacc gatcgcgccc gccattccaa
     4861 gaggtggaat caattttata aatacatagg ccgttattgc ccatacaatc aagctgatag
     4921 taagggcttt actcagccct tttcttgcca tcaggataaa aacgaatgta aagagggtat
     4981 cgatggtcat cccgacaggg atacctttag ttgtggcagc cgcatactcc ggccccattg
     5041 taagtgccat aaatatgagc gatagaagga tactggatgg tagattagta atcagccccg
     5101 ctctcttact gccaagcttt tctccaagga gtgtagcccc ggaaatccac acacccgcca
     5161 gtacaaagga aagggcaatt tccaagacag atctggatat aactacacct ccattctata
     5221 tgctatatcg ttctcggata gcttgctcga catcatcgcg ggtgcccaaa aatggcccca
     5281 gagtttccat tttgaggctt gtcagcgccg ctgcccagag ggtagccgct tttggtggca
     5341 taaagatgcg cgcgctaagg taagaggata gacaagtgtc tcctcggcca cttcttcctt
     5401 ccattgtttt tggcagaaat ggtgcttcga agaaagatcc ttcagcatag accaccacgc
     5461 cacctccgtg tgtcagaacg aactccggaa catcaaatct agcgagaata cgggcagctt
     5521 catacagatc tctagttcct gtcaatatct ccgcctctac gacatctgct ttgaatacat
     5581 ctactagaga gaggacttct cttttctcag gccagtccga gtcatatgca agctgaccat
     5641 ctcgtacgac tcgcacaaat ccctgtgcat cgagacctat acgcgcgttt ttggccttga
     5701 tcgcctttag gacctctaga ctcacttcgc ctcggattga agcgccgata gaccagattg
     5761 gtgctgaaac cgattcgata tccgaaggct cgaaaggctg agctatagaa gtaaccgaaa
     5821 gaatgcgctc gtcagggtta ttcgatggat agaatagtct aaggcatgtc gattttggag
     5881 tagcaatggc tttaaccctt acgcctcgtt ctatgagtgg gctaaagctg ggaaaatctt
     5941 cctctgccag gcgtgttatg gctgctacct tcaatcccat ggcagccgct gcggcagcgc
     6001 catagtagta cgctccgcca tttacaatgc gtgaaccatt tttatcaacg attctgtcct
     6061 ttgtgtactg accc
//
LOCUS       DAOOWY010000108         5973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_13430_length_5973_cov_22.584319, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000108 DAOOWY010000000
VERSION     DAOOWY010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13430_length_5973_cov_22.584319"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1670)
                     /gene="hutH"
                     /locus_tag="PK105_07470"
     CDS             complement(84..1670)
                     /gene="hutH"
                     /locus_tag="PK105_07470"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOU61590.1"
                     /translation="MNEVIIDGCSLSISDVVAVARHKLPVRLDPDAELRMQASREVVE
                     FAVKKKQVKYGITTGFGKFCDVIISEEDNAQLQKNLIMSHACGQGEPFAPEIVRAMMV
                     LRANALAVGNSGVRPMIVHRILQLINEDIVPIVPEKGSLGASGDLAPLAHIALVLIGM
                     GEALYKGTRMAGALALAEAHVQPLVLEAKEGLALINGTQAMAALGVLSVYDALFLYRS
                     ATIASALSFQALRGITEALDPRIHELRRQKAQIEVAQDLRRLLEDSRLTSMASGTRVQ
                     DAYALRCIPQVHGACLAAIRHVWEIVSAELNAVTDNPLIFPYVLREGGDIISGGNFHG
                     EPLALALDYLGIGASELANLSERRLERMVNPTLSEGLPAFLTINGGLNSGFMIVQYSA
                     ASLVSENKVLSHPASVDSIPSSANQEDHVSMGTIAARKARQIIDNARRVIAMELLAAC
                     QALDLRAIQLGFPSIVDSLSPATAQIYTIIRTVVRFSEQDRIMYPDIDAVVRLLSDEI
                     LPQTMERWSWNPEPVVAHEQ"
     gene            1740..2606
                     /locus_tag="PK105_07475"
     CDS             1740..2606
                     /locus_tag="PK105_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU61591.1"
                     /translation="METKRFKASFFGVIVAVIWGLTFLSIKVALREFGPMSLPLFRFV
                     IASLLLALIMILTRTRFRIAWKDVPMLSLSAFSGVTLYYYFENNGIMRLTASESSLIV
                     GTIPVVTLLAEMIAFKIRPRRTVMLGIVLSFVGVALIVMRSENASTSVSGYFFMLGAV
                     LSWVAYGFITKPLSGRYPMLTITFWQMVIGALGCMPFALAEQQSWTGFSTSVLLNATF
                     LAVFGSAIGYWLYIIVLDELGPGRSSVFINLIPVVSVTASFVFLGERLSAMQVAGACL
                     AIAGVYLASSGS"
     gene            complement(2614..3561)
                     /locus_tag="PK105_07480"
     CDS             complement(2614..3561)
                     /locus_tag="PK105_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOU61592.1"
                     /translation="MDNQWIFGIDGGGTSTRLRIESLEGKMLYQAESLSMNPRSVGWN
                     GSRKTLDDLFSGMYKSINLSADNCISGFAGVAGIDREADTGMMRSIIREAGNLGFDTL
                     IEVKNDSIPALAGAFGELRGILLIAGTGSIAVGADSVGRIIRSGGWGHILGDEGSAYW
                     VGLRALNAAIRFQDRRGPQTDLLGRALAYFGEKEAFSLIPAIYEDFDKAKIAAFARIV
                     AEEQDRGDETARQIFIEAAEELALLATSIAIRLGEATIGGKIAFSGGFITNNERLWRD
                     TEARILSTLPRHRVVVPQADATTGACILARSIANQSRLY"
     gene            complement(3663..4313)
                     /locus_tag="PK105_07485"
     CDS             complement(3663..4313)
                     /locus_tag="PK105_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU61593.1"
                     /translation="MIDLYIFDEGGVLIRNHMIMGDVAAALGLDMATFLDFIAPDIKS
                     LSRGSIDSAEFWRRFSDRTGIRPSENYLKTLFRPLPDEDTFSLVDELSKKARVVCGTN
                     TIDSHHEINLQLGLYSPFHAVYASHIIGHIKPEPEFWQHILEAEGVPAERTFFTDDSE
                     ENIKAASSLGIQAVHYIDADHLRQALLELGAPIDRLFGEIGAPVHEADAPIVAPPS"
     gene            complement(4310..5188)
                     /locus_tag="PK105_07490"
     CDS             complement(4310..5188)
                     /locus_tag="PK105_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU61594.1"
                     /translation="MKREASAPSTLWNAVRYKTGSLFATIILIMACSAALLPFAFMFS
                     ASLMPGHDVTTIPYRWIPETPLWSNYLRALKGSDGSWIYLRNIANSFIVASSVALSSV
                     ILASLTGYGLAKFHFKGRNIVFMGIMATMMIPFETVMIPLYLTAVKFGLQNRLIGLIL
                     PFLTSAFGVFLMRQFLLGFPDELLDAARIDGCSEFQIYRSIVFRNATPAIATLAILAF
                     RGQWDNLIWPLLIIQKEELKTIPLYIVLFTSELSTDEGAMMAVAVIAALPVFLLFFTM
                     SKYFLSGAGVYTGSKT"
     gene            complement(5185..>5973)
                     /locus_tag="PK105_07495"
     CDS             complement(5185..>5973)
                     /locus_tag="PK105_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU61595.1"
                     /translation="WTSFHNKKLLSLKPPKFIGLQNWISVLSSQDFWNSVRASAVFCV
                     GVFVPVFLGSLLFALLISSRKKGKRFYQMALYSPAILSSVVTASVWLLIFDPRGLANQ
                     FLNTLLGTPGIDHKWLADAAMVQLSTILVYVWKQIGYYTILFGTGIGKIPSSVVEASM
                     IDGANSWQRLTRITLPLLRPTMALVSVMIFISSLKTFSTQYLFNQHGAPLAPINVLTL
                     NIYRTALSDQNLGKASVMSLFLFFILLVLSWLQVKAGGQGEELE"
BASE COUNT         1525 a         1407 c         1468 g         1573 t
ORIGIN      
        1 cgtagaccat aatcgcgctt tgtcaccata agcaatactc cttgaaaaaa gctccaaata
       61 aaaggggcag acggatttta gactcattgt tcatgggcga caactggttc gggattccag
      121 ctccatcttt ccatggtttg gggcaggatc tcatcgctga ggagtcttac cactgcatcg
      181 atatccggat acattattct atcctgttca gaaaaacgaa ccacggttct aattatggta
      241 tatatttgag ccgtggctgg agatagcgaa tctacgatag aaggaaaacc gagctgaatg
      301 gcgcgcagat ccagagcttg gcaagctgca agtaactcca tggcgattac tcgcctggca
      361 ttgtcgatga tctgtcgtgc cttgcgagct gcgatagttc ccatggatac atgatcttcc
      421 tgatttgcgc tcgaaggaat agagtctacg cttgcagggt gagagagtac tttgttctcg
      481 gataccaggc ttgctgcgct gtattgcaca atcataaagc ctgaattaag tccgccattt
      541 atggtcagaa aggcaggcag accttcggaa agggtcggat ttaccattct ctcgaggcgc
      601 cgttctgaga gattagccaa ctccgaagcg ccaatgccta gataatccag ggccaaggcc
      661 aagggttcgc catggaagtt accaccggaa ataatatcgc ccccttctcg gagcacatag
      721 ggaaagatta gaggattgtc cgtcactgcg tttagctcag ccgagactat ttcccataca
      781 tgccggatgg ccgcaaggca ggcaccatga acttggggaa tgcatctaag ggcataggca
      841 tcttgaactc tagtccctga agccatgctg gtaagtcgtg aatcctccag gagtctgcgc
      901 aggtcttgag cgacctcgat ttgggctttt tggcgcctga gttcatggat acgcggatcg
      961 agcgcctctg ttataccgcg caaggcctga aaagaaaggg cggaggcgat cgttgcactt
     1021 cggtatagaa agagggcatc gtagacagat agtacaccca acgcggccat agcctgggtt
     1081 ccattgataa gggcaagtcc ttcttttgct tccaggacga gtggttgcac gtgggcttct
     1141 gctaaagcaa gagccccagc catccttgtg cctttataaa gggcttctcc cattccgatt
     1201 agcacgagtg caatgtgcgc cagcggagct aagtctccgg aagcgccaag agagcctttt
     1261 tcgggcacaa taggcacgat atcttcgttt atgagctgaa gtatgcggtg tacgatcatt
     1321 ggtcttacac cgctattccc caccgcaaga gcgtttgccc taagcaccat catggctctc
     1381 actatctcag gcgcaaatgg ttcgccttgg ccgcaagcat ggctcattat caagtttttt
     1441 tgaagctgcg cattgtcttc ttcagaaata atgacatcgc aaaatttgcc aaaaccagtt
     1501 gttatgccat attttacttg ttttttcttg actgcaaact ccacaacttc tctggatgct
     1561 tgcatacgca attcggcatc cggatcgagt cgaaccggaa gtttgtggcg agcaacagca
     1621 acaacatcgg aaatagaaag cgaacaacca tcgatgatca cttcattcat gtttttgatt
     1681 ataacataag aagtaccacc catggacaga gattgcctat accgatagca tggttcgtca
     1741 tggaaaccaa aaggttcaaa gcatcgtttt ttggcgttat tgttgctgta atttggggtt
     1801 tgacatttct ttcgatcaaa gtagctctgc gcgaatttgg gcccatgagt cttcctctat
     1861 ttcgatttgt aattgcttcg cttttgctgg ccttgataat gatattgacc cggaccaggt
     1921 ttcgaattgc atggaaagat gttccgatgc tctcactgag cgctttttcc ggtgtaactt
     1981 tgtattacta cttcgagaac aatggcataa tgcggctcac tgcgtcagaa tcgtcgctca
     2041 tagtagggac tattcctgtt gtcacgcttc ttgcggagat gattgcattc aagattcggc
     2101 ctcgtcgcac ggtaatgctg ggaatagtac tctcttttgt aggcgtagcg ctaatcgtga
     2161 tgcgatcgga aaatgcttcg acatcggtgt ctggttattt ttttatgcta ggagcagttc
     2221 tttcttgggt ggcctatgga tttataacta agccgctatc cgggcgctat cctatgttga
     2281 caataacgtt ctggcagatg gtgataggtg ccctgggttg catgcctttt gcgcttgccg
     2341 aacagcaatc ctggactggg ttttcaactt cagtactcct aaacgctaca tttctggctg
     2401 tgtttggctc ggctataggt tattggctct atataatcgt gctggatgag ctagggccgg
     2461 gaagatcgag cgtgtttatc aatctcattc cggtagtatc cgtgactgct tcctttgttt
     2521 ttctaggaga gcgcctcagc gccatgcagg ttgcgggagc ctgccttgca attgcgggtg
     2581 tatatttggc atccagtggc tcataagcaa gatctagtaa aggcggcttt gatttgctat
     2641 tgagcgcgcc aagatgcaag ccccggttgt tgcgtcggct tgaggcacca caacgcggtg
     2701 gcgcggcaat gtggagagta ttcgcgcttc cgtatcgcgc cataggcgtt cattgttcgt
     2761 gataaaacct cctgaaaagg ctatctttcc gccgatggtc gcttctccga gtcggatagc
     2821 aatggaagtt gcaagcaagg ctagttcttc tgcggcttcg atgaatattt gccttgctgt
     2881 ttcgtcgcct cgatcttgtt cttctgccac aatcctggca aacgccgcga ttttcgcctt
     2941 gtcgaaatcc tcgtagatgg ctggaataag ggaaaaggcc tctttttcgc caaaataggc
     3001 aagggctctg ccaagcaaat cggtttgagg cccccttcta tcctggaatc gaattgctgc
     3061 atttagagcg cgaagtccga cccagtaagc cgatccctca tctccaagga tatgacccca
     3121 tcctccggat cggatgattc gacctacact atcggctccc accgcaatag atccggttcc
     3181 cgcgatgagg agaatccctc gaagttctcc gaaggctcca gcaagggcgg ggatagaatc
     3241 gttctttact tctatgagag tatcgaaacc cagatttccg gcttctcgaa tgatgcttcg
     3301 catcatgcct gtatctgctt ctctgtcgat gccagctaca ccagcaaaac ctgaaatgca
     3361 gttgtcggcg gagagattga tactcttgta cattcctgaa aaaagatcat cgagcgtttt
     3421 tctggagccg ttccatccga ctgatctggg gttcatgctg agactttcgg cctggtacag
     3481 catcttgcct tccaggcttt ctatcctcag tcttgtcgat gtacctccgc catctatgcc
     3541 aaaaatccat tggttatcca tcagggacct cgcaagtttt tttcttttat gctagggcat
     3601 tatcaagact agactattct aattgcagga gctaatgcat tcaataagga atttcagata
     3661 attcaagatg gaggcgcaac gataggcgca tcggcctcat ggacaggcgc accgatttca
     3721 ccgaaaagtc gatcgatagg cgctccaagc tctaagagcg cttgccttag atggtctgca
     3781 tcgatatagt gaacggcttg aattcccaga ctagaggcgg cctttatatt ctcttcgcta
     3841 tcgtcggtaa agaaagtgcg ctcggcaggt actccttccg cttcgagaat atgttgccag
     3901 aactctggct caggttttat gtgacctata atatgagatg cgtataccgc atggaatgga
     3961 ctatataatc caagctggag attgatttcg tggtgactat cgattgtgtt agttccgcat
     4021 actaccctgg ctttctttga caattcatcg actaatgaga aagtgtcttc atcggggagc
     4081 ggtctgaata gcgtctttag ataattctcg gaaggcctga tccctgtgcg atcagaaaac
     4141 cttcgccaga actcggcact gtctatcgac cctcttgaga gtgattttat atcaggtgca
     4201 ataaaatcga ggaaagtggc catatccaat cccagggcag ccgctacatc gcccataatc
     4261 atatggttcc gtattagaac ccctccttcg tcgaaaatat agaggtcgat catgttttgc
     4321 tcccggtata gactccagcg ccgctcaaga aatacttgga catggtaaag aaaagcagaa
     4381 aaacaggcaa tgccgcaatg accgctacgg ccatcatggc gccctcgtcg gtgctaagct
     4441 ctgatgtgaa gagtactata tagaggggta tggtcttgag ctcttccttt tgtattatta
     4501 gaagaggcca aatcagatta tcccactgtc cgcgaaaggc gagaattgcc aatgtggcaa
     4561 tagccggtgt tgcattgcgg aatactatag atctgtatat ttgaaactcg gagcagccat
     4621 ctatgcgcgc cgcatctagc agttcatccg gaaaacccaa caggaactgg cgcataagga
     4681 atactccgaa agcgctcgta aggaaaggaa gtatgagtcc gatcagtcga ttctgaaggc
     4741 caaatttaac cgcggtcaag taaagcggaa tcattacggt ctcgaaagga atcatcatag
     4801 tcgccataat tcccataaat actatatttc ttcctttgaa atggaattta gccaatccat
     4861 agccagtaag tgaggcgaga atcacggatg ataaagctac cgatgaagct acaatgaagg
     4921 aattggcaat attccgtagg tatatccagc ttccgtcgga tcctttgaga gcacgcagat
     4981 aattcgacca aagtggtgtc tccggaatcc agcgataagg gatggttgta acatcatggc
     5041 caggcatgag agaagcgctg aacatgaaag caaatggaag aagcgctgca gaacatgcca
     5101 ttatgagaat tatggtcgca aagaggctcc cggttttata gcggaccgcg ttccaaagag
     5161 tgctcggagc ggaggcttcg cgtttcattc gagttcctcc ccttgacccc ctgcttttac
     5221 ctggagccat gaaagaacca atagaatgaa gaaaaggaaa aggctcatta cgcttgcctt
     5281 accgagattt tgatctgaga gcgccgtacg gtagatattt agagtcagta cgttgattgg
     5341 cgccagaggg gcaccatgtt gattaaaaag atattgagta gaaaaagtct taaggctcga
     5401 aatgaagatc ataaccgaca ccagcgccat agtgggcctc aataaaggca gggttattcg
     5461 agttaatcgc tgccagctgt ttgccccatc gatcattgag gcctctacaa cgctagaggg
     5521 aatctttccg attcctgttc caaaaagtat ggtatagtat ccaatctgct tccatacata
     5581 gacgaggatt gtcgatagtt gaaccatcgc ggcgtccgca agccacttgt gatcaatccc
     5641 cggcgtgccc aatagggtgt tgagaaattg atttgcaagg ccgcgaggat cgaatatgag
     5701 cagccacacg cttgccgtga cgacagaaga tagaatagcc ggggaataaa gtgccatctg
     5761 gtagaatcgc ttgccttttt tgcggctcga gattaaaagc gcaaacagca aggatcccaa
     5821 aaacaccgga acaaagacgc ccacacagaa cacagccgaa gctcgtacag aattccaaaa
     5881 atcttgcgaa ctcagaaccg atatccaatt ctgtaaaccg atgaattttg gtggtttgag
     5941 tgaaagcaat ttcttgttat gaaagctagt cca
//
LOCUS       DAOOWY010000109         5771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_14058_length_5771_cov_13.796186, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000109 DAOOWY010000000
VERSION     DAOOWY010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14058_length_5771_cov_13.796186"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PK105_07500"
     CDS             <1..243
                     /locus_tag="PK105_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOU61596.1"
                     /translation="AIDLLPRRWQNKIIYWNWEKNKGNEDWMLIPLSERWMPPDIVID
                     IFEKEGFIWGGKWTVWDNMHFEYRPELILGRELVKP"
     gene            573..1808
                     /locus_tag="PK105_07505"
     CDS             573..1808
                     /locus_tag="PK105_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61597.1"
                     /translation="MQRKLLFAVLATMLIFSSAAQEKIIKIGTLFPMTGSVATAGQRC
                     TAAVETVVDIINGNYPNLDLPIAKKAGLLDGYKIVLVKADHQGKPDVAKSEAERLYEQ
                     EKVFAIIGCYNSSASKPASAVAERLQKIFMCGSSSSAELTERGYNYFFRLAPTDRIES
                     YEFVEWFQELNKSRNANIKTASVIYENTEFGKHAADEAKAACAKAGIKIIGDVPFSPG
                     ATNLNSEIQKLKAANADALFGAVLGADYSLMVRTMKQINWKPKIAINYCSGYQDPNIM
                     QQLGKDGWYFMGSAGYTPEFGLRFMKAVAPVEEIYKKKSNVPFDSNSIQEAVAMYVLA
                     LAIEKAGAVDTEKVVQVLRTTTFDSPLSMGGKVQFESPGGQNIKAQSVISQIQNGSYV
                     TVYPYEYKDGDPIYPMPAW"
     gene            2036..2893
                     /locus_tag="PK105_07510"
     CDS             2036..2893
                     /locus_tag="PK105_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61598.1"
                     /translation="MSLFFQILLDGLWSGLLYALISSGLSLIWGVLDVINFAHGEFLM
                     VGMYVSFFLGFLFKMDPLISWVASGAFLFILGVLTYKLVIKRTLKMAMAPLLASFGLS
                     MVLKNIAMNRFTPNFRILSGTIMEGKVISLGSVIIPVPNLVTAIFSLVILGILYWMLT
                     KTRFGWAVQATAMDPEAAQLMGINTEHIFVLVFGLGGACVGIAGGLLPSYLATHPDVG
                     TLFGLIAFIVVALGGFGSIPGALVAAILVGLIESFAGFYIAPVFKYIAVFGVYLVVIL
                     IRPKGIKGW"
     gene            2897..3961
                     /locus_tag="PK105_07515"
     CDS             2897..3961
                     /locus_tag="PK105_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61599.1"
                     /translation="MQKSEYQDCRQKKPKFIPWLAVLLVLALLPIIGIIKGNFLSHVL
                     VMILLNASMAQAWNIIGGYAGQISFGHSVFFGIGAYGAAYAVTTLKITPYPGMIVGAL
                     IACVVALIIGYPTFRLKGHYFAIATFAIVEVFNRLFMIWDTVGGAIGLDYPILKDGWR
                     NFTWSRTKVGYYYGALILFVLIFSIVRTMEHAKFGYYLKAIREGQETAESLGVNSRRY
                     KLLAMALSAAMAAFCGAFFAQYNYRVDPSMVLSLDMSMKFVLITILGGIGTLWGPLLG
                     AAILIPLQEYTRAYLARLGPGIDLIIFGIIIILMMEFQPRGLMGILENLRKPSRALVK
                     RSNTNSVQNNRPKGHLGGKQ"
     gene            3958..4746
                     /locus_tag="PK105_07520"
     CDS             3958..4746
                     /locus_tag="PK105_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61600.1"
                     /translation="MSALLEIRNVTMKFGELVADRDVSFNVETGTICGLIGPNGAGKT
                     TLFNCIAGYYKPSKGEIFFKGNRISGLPPHAITKLGIARTFQVVKPLKEMSVLDNIMV
                     GAFLHHSGASEARKIAQYCLSLCFLEDFSDKPAGELPIGSKKRLEIARALATEPSLLL
                     LDESISGLTSTEIHEMLEMIRRLRDKGITILMVEHIMEAIMPLANTIVVLSHGEKIAE
                     GQPQSIVHNQEVITAYLGEKFVKKMNMQNQIMLTDAGDKKGDTP"
     gene            4743..5459
                     /locus_tag="PK105_07525"
     CDS             4743..5459
                     /locus_tag="PK105_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61601.1"
                     /translation="MSEIILEVKDIHCEYDNVPVLHGVSLRVCKGEIVAMVGSNGAGK
                     TTTLRTIAGLLHPVSGSIRFMGKDISRTEAYALVRNGISYIPEGRRLFGKLTVRQNLE
                     LGAYTTKERSIIQKRLHEVFQLFPILDERQNQTAETLSGGEQQMCAIARGLMSDPALL
                     LLDEISLGLAPSLVEAVFDTIIKIRDQGITILLVEQLVQESLEIADRGYVIQTGRIVH
                     DGPSHELLEMPEIRAAYMGM"
     gene            complement(5583..>5771)
                     /locus_tag="PK105_07530"
     CDS             complement(5583..>5771)
                     /locus_tag="PK105_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61602.1"
                     /translation="CWNPSPLLEYIGQYIQSIQIRLTHQAEKEHYLTKSEIVIFEFLI
                     VFQHWEIGPKKITRANKA"
BASE COUNT         1590 a         1257 c         1363 g         1561 t
ORIGIN      
        1 gccatcgatc ttttgcctcg ccgatggcaa aacaaaatta tttactggaa ttgggaaaag
       61 aataaaggca atgaggattg gatgctcatt ccactgtccg aacgctggat gccccctgat
      121 atagtcatcg atatattcga aaaggaaggc tttatctggg gtggcaaatg gacagtctgg
      181 gataatatgc atttcgagta tcggcctgag ttgatcctgg gaagggagct tgtaaagcca
      241 taattgcagt actttcccta tgcatgaatc agattgatgc atgcatcaga ttggttcata
      301 gatttcattt tccgccacga tttgtgcggg aaaagctgtc caggaagctc tgcgaaaccg
      361 gccagatcca aaatcgggga atcccctatc tctttatcaa ctaggcaact atttcaaaaa
      421 gtatttgaca gcggtgcaag aacgattcta tactacgaaa atcgatcgag cctatttcaa
      481 tatcaattga gcccaccata agaaggttat ttgcaagcag attgcttgcc agatatatga
      541 gggatcagtt ctatttaata ggaggtaaga atatgcaaag gaagctattg tttgcggttc
      601 ttgcgacaat gcttattttc agctcagcag cacaggagaa aatcatcaaa attggaaccc
      661 tgttcccgat gacaggatca gtcgccacag caggacaacg ttgtacggcg gcggtagaga
      721 ctgtcgtcga tatcatcaat gggaattatc cgaatctcga cttgcctatc gccaaaaaag
      781 caggactgct ggacggatat aagattgtgc tcgtcaaagc agaccatcaa ggcaagcccg
      841 atgttgccaa atcggaagca gaacgtctct acgaacagga gaaggtcttt gcgataattg
      901 gttgctacaa tagctccgcc tcgaagcccg ccagcgccgt ggccgaacgg cttcaaaaga
      961 tattcatgtg tggttcctca agttccgctg aacttacaga gcgtggctat aattacttct
     1021 tcagactggc gccgacagac cgaatcgaaa gctatgagtt cgtagagtgg ttccaggagc
     1081 taaacaaatc gcgaaacgca aacataaaga cagcctcggt gatctatgag aacaccgagt
     1141 tcggcaagca tgctgccgat gaagcaaagg ctgcttgtgc caaggcagga atcaagatta
     1201 tcggcgacgt tccattttca ccaggtgcaa cgaacttgaa ttctgagatt caaaaattaa
     1261 aggccgcaaa cgcagatgct cttttcggtg ctgtccttgg cgccgattat tcacttatgg
     1321 tcagaaccat gaaacagatc aactggaaac ctaaaattgc catcaattat tgctccggct
     1381 accaggatcc aaatatcatg cagcagcttg gcaaggatgg atggtacttt atgggttctg
     1441 caggatacac gcccgagttc ggattgcgct tcatgaaagc cgtcgcgcca gtcgaggaga
     1501 tctataagaa gaaatcaaat gtccccttcg attccaattc gatccaggaa gcagtagcga
     1561 tgtatgttct cgcgctggca atcgagaaag caggcgctgt ggataccgaa aaagtcgtac
     1621 aagtactccg tacgacaacc ttcgattcgc ctctctcgat gggtggaaaa gtacaattcg
     1681 aatctcctgg cggccagaat atcaaagccc agagcgttat ttcgcagatc caaaatggca
     1741 gctatgttac tgtctatcca tatgaatata aagatggcga tcccatttac ccaatgccgg
     1801 cttggtaatc attctgaatt atcgtccaag cttgcttcta atcgagaatg atatgatgat
     1861 tgtgcagtat tccgataatt atagaaggag tatagaataa cagggatata actaaaagag
     1921 cggctttgca cctgcagagc cgctcttttg tcatgcaaaa ggcttttccg aaatatgcca
     1981 gagcattcaa aatgagtctc ctttttaaaa tgcagcatgt tcctgaggag gtagaatgag
     2041 cctttttttt cagatattgc tggatgggtt atggagcggt cttctctatg cccttatttc
     2101 atcaggactt tccttgatct ggggcgtact ggatgtcatc aattttgcac atggtgagtt
     2161 tctcatggtt ggaatgtacg tctcgttttt tctggggttt ctcttcaaaa tggatcccct
     2221 aatctcatgg gtcgcatcag gggcatttct tttcattctt ggagtgttga cctacaagct
     2281 tgtgataaaa cgaacactaa agatggcaat ggctccgcta ttggcgagct ttggcctgtc
     2341 aatggtactc aaaaatattg ctatgaatcg atttacgccg aatttccgta ttctctccgg
     2401 aacaattatg gaaggaaagg tgatatctct cggttccgta atcattccag tccccaattt
     2461 ggttaccgcg attttttcct tggtgatact aggaattctt tactggatgc taactaagac
     2521 tagattcggc tgggcggtcc aggctaccgc tatggatccg gaagcagcac agcttatggg
     2581 gatcaatacc gagcacatat tcgtccttgt atttggcctt ggaggagcgt gtgttggaat
     2641 tgcgggaggg ctcctgccat cctatcttgc cactcatccg gatgttggga cgctctttgg
     2701 actcatcgca tttatcgttg tggctctcgg tggctttggg agtattcccg gtgctttggt
     2761 agcagccatt cttgtggggc ttatagaatc ttttgccggt ttctatatcg cacctgtttt
     2821 taaatatatt gcggtgttcg gtgtctattt ggtagtcatt ctgattcgac caaagggcat
     2881 aaagggatgg tgagctatgc aaaagagcga atatcaagat tgtcggcaaa aaaagcctaa
     2941 gttcattcca tggctcgctg ttcttcttgt ccttgccctt ctgcccataa ttgggataat
     3001 aaagggaaat ttcttatctc atgtcctggt aatgattttg ctaaatgctt ccatggctca
     3061 agcatggaac ataattgggg gatatgccgg acagatatcc tttgggcatt cggttttctt
     3121 tggaataggc gcatacggcg cagcttatgc tgtaacaact ctaaagatca ctccttatcc
     3181 tgggatgatt gtgggcgcgc tcattgcgtg tgttgtcgcg ctcataatcg ggtatccaac
     3241 tttcagatta aaagggcact attttgcgat tgccacattt gcgatcgtgg aagttttcaa
     3301 tcgtctattt atgatctggg atactgtagg gggcgcaatc ggcctggatt accctattct
     3361 caaagatgga tggcgaaatt ttacctggtc aagaacaaaa gtggggtact attacggtgc
     3421 ccttattctc tttgtgctta tcttcagtat cgtgcgaacc atggagcatg caaaattcgg
     3481 atattatctc aaagcgattc gagaaggcca ggagacagca gaatccttag gagtcaattc
     3541 tcggcgctat aaacttttag ccatggctct ctctgcagct atggcggctt tctgcggcgc
     3601 attctttgct caatataatt acagagtaga tccttcaatg gtgctctccc tcgatatgtc
     3661 catgaagttt gtgctgataa cgattcttgg aggcatcggc accttgtggg gaccattact
     3721 cggcgctgcc atactgattc ctttgcagga atacacccga gcatatcttg ccaggcttgg
     3781 gccgggtatc gatcttatca tttttggaat catcataata ctcatgatgg aattccaacc
     3841 tcgagggcta atgggaattc ttgaaaatct cagaaagcca agccgtgcct tagtcaagcg
     3901 gagcaatacc aattctgtgc agaacaatcg tcctaaagga cacctaggag gcaaacaatg
     3961 agtgcccttc tcgagatccg caacgtgact atgaaatttg gcgaactcgt ggctgatcga
     4021 gacgtaagct ttaatgtcga aactggcact atctgcgggc ttatagggcc aaacggagca
     4081 ggaaaaacaa cactcttcaa ttgcatcgca ggttactaca agccatccaa aggcgaaatc
     4141 ttcttcaaag gaaaccgaat ctccggcctt cctccacatg caatcacaaa actaggtatt
     4201 gcgagaacct ttcaggtcgt aaagcctctc aaggaaatga gcgtcctgga taacatcatg
     4261 gtaggtgcgt ttttgcatca ttcgggagct tccgaagcac gcaaaatcgc ccagtattgc
     4321 ctttctctct gctttcttga agattttagc gataaaccag ccggagagct tcccataggc
     4381 agcaagaaga gactagagat tgctcgtgcc ctcgcaacag agccctctct tcttttgctc
     4441 gatgaaagta tttctggact tacaagtacc gaaatccacg agatgctcga aatgataaga
     4501 agacttcgag acaagggcat aacaatcctg atggtggagc acatcatgga agcaattatg
     4561 cctcttgcga atactattgt tgtcctaagc catggcgaaa aaatagcgga aggacaacct
     4621 caatcgatag tgcacaatca agaagtgatc accgcctatt tgggcgaaaa attcgtcaag
     4681 aaaatgaata tgcaaaatca aatcatgctc acagacgctg gggataaaaa aggagacacc
     4741 ccatgagcga aatcatccta gaagttaaag atatacattg cgagtatgac aatgtaccgg
     4801 tgctacatgg ggtttcatta agagtatgca aaggagaaat cgttgcaatg gtaggctcga
     4861 atggtgcagg aaaaaccaca actctcagga caatcgctgg tctcttgcat ccagtttctg
     4921 gcagcatccg cttcatggga aaagacataa gcagaaccga agcctatgcc ctggtaagaa
     4981 atggcatctc ctatattcca gaaggccgta ggctctttgg taagctcact gtaagacaga
     5041 acctggagct aggcgcatat actacaaaag agcgctcgat aattcaaaaa cgcctccatg
     5101 aagtatttca gcttttccct attcttgatg aaagacagaa tcagactgca gagacccttt
     5161 cgggcggaga acagcagatg tgtgcaatcg ctcgtggctt aatgtccgat ccagctctcc
     5221 tcctacttga tgaaatatca cttggccttg cgccctcctt ggtagaagct gtattcgata
     5281 ccatcattaa gatacgagac cagggcatca ctatcctcct agtcgaacaa cttgttcaag
     5341 aatccttgga gatagccgat aggggctatg taatccagac aggccggatt gtgcatgatg
     5401 gaccctcgca cgaacttcta gaaatgccag agattagggc agcttatatg gggatgtaaa
     5461 ggattcgagg tcagtggctg gcagaatgtg ggataaccat aaatgcaaaa tactatgaaa
     5521 tccgcttgct ggtgatatta ttgcaaagcc tggcaaagct ctaattggtt ctagaataag
     5581 gattaagcct tattagctcg tgtgattttt ttaggtccaa tttcccaatg ttgaaagacg
     5641 atcagaaatt caaatattac gatttccgat ttggtaagat aatgttcttt ttcagcctgg
     5701 tgcgtaagtc gtatctggat cgattggata tattggccta tgtattcgag taaaggggat
     5761 ggattccaac a
//
LOCUS       DAOOWY010000110         5751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_14128_length_5751_cov_13.848315, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000110 DAOOWY010000000
VERSION     DAOOWY010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14128_length_5751_cov_13.848315"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1938)
                     /locus_tag="PK105_07535"
     CDS             complement(100..1938)
                     /locus_tag="PK105_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61603.1"
                     /translation="MRLVARKHNLDTRYSALVFCMALIFSAVSREANAQENAAWNVVK
                     VKSGLQGNLQVQKPIHAADLFFPGDDEYDRAFSAAIRQGRLPKTNVFPLFGGELGASL
                     EAEIAEPELLSGIVFRASTPTLYYDSPAKQKDLGTSLLEDPSLDVNTFPEILHFWQTL
                     PDVLALGLNLYPSSSIAFKIDISFNYRRDKIIGQSSPFQWDINDLRENLTSAAQFPRR
                     TYISWVGKGYGVAMGRFPSGMGWGMLSGSILNPRASWYDQIRFYFESGQWRFTSMTAT
                     SSAQLSKAEQEIQFRKNSDGSSFWDSVSDHDHASHDMAIKLANWHQIEWKPAPWLELS
                     VAEMSVIGGRMPSLSFVLPTTIWHNTYAAGYSNVGVSLSAAIVPFNGLMLSGELFIDD
                     LKGAREKQSADSKPQSLAWLGTARWSALLFDTISLDTGLEYQHVDRWTYVRWSPYLAM
                     YQRQTLPDGESGQDQSLGAPWGPDYDSIGAYTSMKFKSGAYLRLSYEFIRKGPIYQGM
                     ASQTSVSADFDGDGTIDSAQQKIWVPVYYDYDEYAGDGALEAILSKPDEYRHLVSLMG
                     AVPLAKSIELQLSATLGLYRNFGHVAGASEEAFLLYAGIVVYLPAK"
     gene            complement(1983..3107)
                     /locus_tag="PK105_07540"
     CDS             complement(1983..3107)
                     /locus_tag="PK105_07540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU61604.1"
                     /translation="MSEALKASIYRDLDTPAAIWDSLAQDSRIYAFQTRHWLSVWQNT
                     IGNAHGVVPCIIVFEKGQNTALFPLGKKKVRFGTSSRTMLIWLGYGVSDYAAPIFSKE
                     AQFDMTELFAHLRSIAKKEHCCAVYLDRIPKFWADGTAVLFVQALLALPFGHRLHYSA
                     HAMHLSQDPLLRLNAKERAHLRRAERRLSEAGTLSFIIAKDKSLRKRLTCSMIAFKQA
                     RFRQMKVHDNFVDPAFGDFYIQASQDEHIPLHICALMLNERPLAVHWGLSCLQRLYYL
                     MPAFDIEKYPSLSTGNLLLVKLLQYCAGNNYRLVDFANGDEPYKEKWCDMELALYEGY
                     IPSEPAGWLISLFESALESIKRSTLAPHLKRMAQFLKFSA"
     gene            complement(3104..4681)
                     /locus_tag="PK105_07545"
     CDS             complement(3104..4681)
                     /locus_tag="PK105_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61605.1"
                     /translation="MKKLFLPAIVILLFLIFSGIFTLSAQQLSQSAAQQPGQSAAQQS
                     EELAEQGLLFTLATELPYRAGTYREEIEARGLTPNASISLQYEQTHIGLIAAIDLTSD
                     QVYGPWLADSPTGALGDLNFLIQEAGMRISGGSFNLKAGRLRHFDVVDSPYSLFINAN
                     GIAAPMIDFVYRDSRFFYESRFISLTLDSAMHTTAWPKASYTGFPDRGANFKVFGLSF
                     GRLTIGFEDSSVYSGRAFDLEYFLSPMPQYFTQYVRGTAGRPWYANYDDNYNAGLFVT
                     WKEPGAYDLYAQAFVDDLGMLGLFGWTNNPWQIALALGGRIKTPKGTFGLHVAGATKY
                     TFEPGDMRNASTENQIQSSYGYTYFPETRFEYEWRSGYPTSYKAIAPELNLIGYQYGE
                     NNIALRLDWSKAVNRFDCDAFFEFRLSGSNSPANPWHDLWIDPQVAFTWLDDPVLEKR
                     FTISARAITAREPWQFFAKATGVLALDALELRDPSGVPSTQTEVDQKVQIYSPVAGNT
                     KLLGELTFGVVLRLGIQ"
     gene            complement(4766..>5751)
                     /locus_tag="PK105_07550"
     CDS             complement(4766..>5751)
                     /locus_tag="PK105_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61606.1"
                     /translation="YDALRAQGKLGCFAFDWLVSTIQAVQAWDKNDITPEVGYGFEDQ
                     PGTPTIIVEAMNRFSWHFGAFSVGVTDHAMMSRLNNHFVLTDFFPIGSRHQASVAQTN
                     NSMVFDASWEPFDGLAIAAQLGLDDIDLNVLVEGSDTGSPTIPAYLAAVRYDGEVRTL
                     PYAFYLEAGSTHWLWGNYDGSQAWPTIGDVNYFARFIYRFPRYLGGSILLPLTSPYGP
                     GAAWIQSHGELIIPIGSENDRHPLSFGVGYRFLFLDMNTDANLITTQVYNNSTTPAAP
                     HWQSFFISLPLRLSAGLFSLDIAPSFVGSGGSTGFELDIALSGTIAFATVH"
BASE COUNT         1522 a         1402 c         1437 g         1390 t
ORIGIN      
        1 gtatgtgcca gcctatgcct atgcttgaga caattcctta tagaatgcct aaagtgtaca
       61 caaggttcgt agcagtaaaa aaggttcgct gcagtaaagc tattttgcag gtaaatatac
      121 aacaatccct gcgtataaga gaaaagcctc ttcgctagcg ccagcgacat gcccaaaatt
      181 gcgatataac cccaaggtcg cggatagctg cagttcaatg gactttgcaa ggggcactgc
      241 tcccatgagc gatacaagat ggcgatattc gtcaggcttt gagagtattg cttccagggc
      301 accatctcct gcatattcgt cgtagtcata gtagacaggc acccagattt tttgctgcgc
      361 cgagtctatt gttccgtcac catcgaagtc ggcacttacg ctcgtctggc ttgccattcc
      421 ctggtaaata ggcccttttc ggatgaattc gtaggagagg cgtagatagg cgccgctttt
      481 gaacttcatg cttgtatatg cgcctatgct atcataatca ggaccccagg gagctccgag
      541 cgattggtct tggccagatt ctccatctgg aagcgtttgg cgttggtaca tggccagata
      601 agggctccag cgcacatagg tccagcgatc cacatgttga tactcaagac cagtatcaag
      661 agaaatcgtg tcaaaaagca atgccgacca acgtgccgtt ccaagccagg caagggattg
      721 aggcttggaa tccgcagatt gtttttccct tgcgcccttc agatcatcaa taaaaagctc
      781 tccagatagc attagcccgt taaaaggcac tatggctgcg ctcagcgaga cgcccacatt
      841 ggagtatcca gccgcatagg tgttgtgcca gattgtggtg ggcaaaacaa agctgagaga
      901 gggcattctt ccgccgatca cgctcatctc cgcaaccgaa agctctaacc acggcgctgg
      961 cttccattcg atctgatgcc agttggccag ctttattgcc atatcgtgcg acgcatggtc
     1021 gtggtcgcta actgagtccc aaaaactgct tccatcggaa ttttttctga attgaatttc
     1081 ctgctcagcc ttggagagct gggccgagct tgtcgctgtc atactcgtaa agcgccattg
     1141 accggattcg aaatagaagc gaatttggtc gtaccaggaa gctctcggat tcaagatcga
     1201 accggacagc ataccccagc ccataccaga aggaaatctg cccatggcca caccatagcc
     1261 tttgcccacc cagcttatat aggttctccg cggaaattgc gcagcagatg taaggttttc
     1321 gcgcagatca ttaatatccc attggaatgg cgagctctgg cctattattt tgtcgcgtct
     1381 atagttgaac gaaatatcta ttttgaatgc gatcgaagac gaagggtaca gattcaaacc
     1441 aagcgctagc acatccggca atgtctgcca aaaatgcaag atttccggaa aggtattgac
     1501 atctaggctt ggatcctcaa gaaggcttgt gccgaggtcc ttctgctttg ctggcgaatc
     1561 gtaatatagg gttggcgtgc ttgccctgaa gacaatgccc gaaagaagct caggctccgc
     1621 tatctcggct tccaaggaag ccccaagctc accaccaaag agggggaaaa cattggtctt
     1681 aggtaggcgg ccctggcgta tagcggccga aaaagccctg tcatattcat catcgccagg
     1741 aaagaaaaga tcggcagcat ggatgggctt ctggacttgc agattgccct gtagacctga
     1801 ttttactttg acgacattcc aggcagcgtt ctcttgtgca tttgcttctc gactaacagc
     1861 gctaaagatc agcgccatgc aaaaaaccag ggcagaatac ctagtatcca agttgtgttt
     1921 tcttgctact aaccgcattg tatcccttct ctattacttt attcagtgct tggatctctg
     1981 gatcatgctg agaattttag aaattgtgcc attcttttga ggtggggtgc aagagtgctt
     2041 cgctttatgc tctccaaagc gctttcaaat agggaaataa gccatcccgc tggttcggat
     2101 gggatatagc cttcatatag ggctagctcc atatcgcacc acttctcctt atagggttca
     2161 tcaccattgg caaaatcgac gagccgataa ttatttcccg cgcaatactg caagagcttg
     2221 acaagaagca ggttgcctgt cgacagcgaa ggatatttct caatatcgaa ggcaggcata
     2281 aggtaatata ggcgctgcag acagctaagc ccccaatgca cggctagagg cctttcgttc
     2341 agcatgagcg cgcatatgtg caagggaata tgttcatcct gcgaggcttg gatataaaag
     2401 tcgccaaatg cggggtcaac aaaattatca tggactttca tctgccgaaa gcgcgcttgc
     2461 ttaaaagcga tcatagagca agtaaggcgc tttcgaagcg atttgtcttt ggcgataata
     2521 aaggataaag taccagcctc tgatagacgg cgctcggctc tgcggaggtg cgcacgttct
     2581 ttggcgttga gacgcaataa tgggtcttgc gaaaggtgca ttgcgtgagc gctataatgt
     2641 aggcggtgtc cgaaaggcaa cgcaagcagt gcttgaacaa acagaaccgc cgtgccatca
     2701 gcccagaatt ttggaattcg gtcgagatac accgcgcagc aatgctcctt ttttgcaatg
     2761 ctcctgagat gggcgaagag ctctgtcata tcgaactgtg cctccttcga aaatatcggc
     2821 gcagcataat cagacacgcc atatccgagc catatgagca ttgtgcggga agaagttcca
     2881 aatcgcacct ttttctttcc aagaggaaac agggctgtat tctgaccttt ttcaaagacg
     2941 atgatacaag gcacaactcc atgggcattc ccgatggtgt tctgccatac tgaaagccag
     3001 tggcgagtct gaaaggcgta gatgcgtgaa tcttgtgcta ggctatccca gatcgccgcg
     3061 ggtgtatcga gatctcggta aatcgaggct ttgagcgctt cgctcattgt atcccaagcc
     3121 tcaagacgac tccaaaggtg agttcgccta gaagtttcgt gttacccgca acaggagaat
     3181 agatttgcac tttttgatcg acctctgtct gggttgatgg aacgccgctt gggtcgcgca
     3241 gttcgagcgc atccagagca aggacgccgg tggcctttgc gaaaaactgc cagggttcac
     3301 gtgctgtaat tgctcgggcc gaaatggtaa atcttttttc gagcacagga tcatcgagcc
     3361 aggtgaaggc cacttgtgga tctatccaaa gatcgtgcca ggggttggcg gggctattcg
     3421 agccagacag cctgaattca aagaaggcat cacagtcgaa tctgttgaca gccttgctcc
     3481 aatcgaggcg caaggcaata ttgttctcgc catattggta accgatgaga tttagttccg
     3541 gagcaatcgc cttataactt gtgggataac cagaccgcca ttcgtactca aaacgggttt
     3601 ctggaaaata ggtgtagcca taagagctct gtatttggtt ctctgtggaa gcatttctca
     3661 tatccccggg ctcaaaggtg tattttgtcg caccagctac atgaaggcca aaagtcccct
     3721 taggcgtctt tattcttcca ccgagggcga gcgcaatctg ccaagggttg ttcgtccatc
     3781 caaagagtcc caacatccca aggtcgtcga caaaagcctg cgcatacagg tcataagccc
     3841 cgggctcctt ccaggtgaca aataatcctg cattatagtt atcgtcgtag ttggcatacc
     3901 agggccgacc tgcggtgcct ctgacatatt gggtaaaata ctgaggcatt gggctcagga
     3961 agtattccaa atcgaatgct cggccggaat acaccgagga gtcttcaaag cctatcgtaa
     4021 gcctcccaaa agaaagacca aaaaccttga aattggcccc acgatcggga aaaccggtat
     4081 aggaagcctt aggccacgcc gtcgtgtgca ttgccgaatc gagagtgagt gagataaagc
     4141 gcgattcata aaagaatcgg ctatctctat acacaaagtc gatcattggc gccgcgatac
     4201 cattggcgtt gataaagagc gagtacggtg aatccacaac atcgaaatgg cgaagcctgc
     4261 cggctttgag attaaatgag ccgccggaaa ttctcatacc cgcttcctgg attagaaaat
     4321 taagatctcc aagcgcaccc gttgggctgt ctgccagcca agggccatag acctgatcgc
     4381 tggtgagatc gatggcggct atgaggccaa tatgagtttg ctcatattga agagatatgg
     4441 aggcatttgg tgtaaggcct cgtgcttcga tttcctcgcg atatgtaccc gcgcggtaag
     4501 gcagctctgt ggcaagagta aagagaagac cttgctcggc aagctcttcg gactgttggg
     4561 cagcgctttg gcctggctgc tgggcagcgc tttggctgag ctgttgtgca gacaacgtaa
     4621 aaattcccga gaagataaga aataaaagaa tcacaattgc tggcaaaaat agttttttca
     4681 tctatattcc tacgatccat cattaagtct ataacagttc gatctattca cagcaaagtc
     4741 tactgcatac acaaggttcg tatcactaat gaacagttgc gaatgctatg gtacctgaga
     4801 gcgcaatgtc aagctcgaag cccgtcgaac caccacttcc gacaaaactc ggtgcgatat
     4861 ctaaagaaaa caaacccgcg gaaaggcgca aaggcaggct tatgaagaaa ctctgccaat
     4921 gtggagccgc aggcgttgtg ctgttattat agacttgagt cgtgataagg ttggcatctg
     4981 tattcatgtc gaggaataaa aagcgatagc caactccgaa gcttagagga tgcctatcgt
     5041 tttcgcttcc aattgggata atgagctcgc cgtgactctg tatccaagca gcaccggggc
     5101 cgtaaggact tgtaagaggc aagagaatag accctccaag atatcgtgga aaccggtata
     5161 taaagcgagc aaaataattg acatctccaa tagtaggcca ggcttggctt ccgtcgtagt
     5221 ttccccaaag ccaatgagtg gaaccagcct caagatagaa tgcataaggc aaggttctta
     5281 cttcgccatc ataacgtaca gccgccagat aggctggtat agtaggcgaa cctgtatctg
     5341 aaccctctac gagcacgttc agatcgatat catcaagacc aagctgtgca gctatggcaa
     5401 ggccatcaaa aggctcccag cttgcatcga acaccatgga gttattggtc tgcgccaccg
     5461 atgcttgatg gcgcgagcct atcgggaaaa aatctgtcag cacaaagtgg ttgttgagac
     5521 gagacatcat ggcatgatct gttacgccca cggaaaacgc accaaaatgc caggaaaatc
     5581 gattcattgc ctctacaatt atggtaggcg tgcccggttg gtcctcaaag ccatacccaa
     5641 cctccggcgt gatatcattt ttgtcccatg cctgcactgc ctggattgtg gacacaagcc
     5701 agtcgaaagc aaagcaacca agttttcctt gagctcgtag cgcatcgtaa t
//
LOCUS       DAOOWY010000111         5625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_14543_length_5625_cov_9.732855, whole genome shotgun sequence.
ACCESSION   DAOOWY010000111 DAOOWY010000000
VERSION     DAOOWY010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14543_length_5625_cov_9.732855"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..606
                     /locus_tag="PK105_07555"
     CDS             199..606
                     /locus_tag="PK105_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61607.1"
                     /translation="MESVLYENRLHYYEAIEQARKVDDSGAFIEFTLSAILDTITPQV
                     EHQVKHAVEHQVELSDIQYAVLKSLEYKSLSRKEIFAAIRRNGDSRSFKRIVELLIVT
                     GLVEMTAPDKPNSRLQKYRLTHQGKMALKENMP"
     gene            699..2045
                     /locus_tag="PK105_07560"
     CDS             699..2045
                     /locus_tag="PK105_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61608.1"
                     /translation="MIDPGVELNMEPGQLALQHDWHFLGFSNAPFSVSDEEVLDHIRA
                     IQRAGQIKPSEKLEGHYNLTIEMETGVGKTYTYIKTMYEFNSQPFNARGAGYFSIDKR
                     SGRMIDSTLGNRRERTSDDADAYDLIMKDKERLLDRRERMDENLIAREEIHTVNVLTV
                     VANESYESFAKGLQSEIAEVVADRPRKVEAKLFTDDFGEDTALVIYESLIEYGYVKRG
                     ELTEKFFEDKKNDKIELPQEAANRTADVIKVLDSVYDPNAIKPENARSNTVQVTLDES
                     KFNSRAFQELWSRINQKSYYIVNFDEKELVSKAIAELNAKLRVSKVYFKVEKGEQVRT
                     IESKTQLVEGAAFVRAEVARTPAMPYTVMKASNSVRYDLVGKYSPDWAIAFYEGTVKH
                     IYFVAETKGDMATLELREIETTKAHCAREHFRAISGENVKYEVVDSYDKLWEIVSK"
     gene            2243..3160
                     /locus_tag="PK105_07565"
     CDS             2243..3160
                     /locus_tag="PK105_07565"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF006143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOU61609.1"
                     /translation="MELKDISKVLIVGAGTMGQQIAFQCAAHGYGVTICDVNASALAV
                     GLHRLSAYADELVAGGYMDRQTAEKAVTQIAATTDETDAAHEADLLSESIPEDPALKR
                     KVFARFNELCPSRTIFTTNTSMLIPSVIAESTGRPERFLAFHFHQPVWVGNVADIMPH
                     PGTATEIVKLVRDFARSINQIPMVLRKENHGYVFNAMYGSLNCAAIALVANGVASVQD
                     VDRAWMGIMKMPIGPLGMLDNVGLDTVLHISETSDVDCKDEQNRTNIEFLKKEYIDKG
                     WLGTKTGRGFYTYPNPAYQDPDFLLVDEN"
     gene            complement(3580..3717)
                     /locus_tag="PK105_07570"
     CDS             complement(3580..3717)
                     /locus_tag="PK105_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61610.1"
                     /translation="MTKQVPGNYTIRASLEIPSQEVLRRFSLDSPWRYEGRNIDDEEK
                     T"
     gene            4337..>5625
                     /locus_tag="PK105_07575"
     CDS             4337..>5625
                     /locus_tag="PK105_07575"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOU61611.1"
                     /translation="MNIRTTTSVQGSKTYHYVQLVQSVRDEKTGKPKTIVLYNFGKPE
                     DVDLDAVRKLIAALSTLLPHGELVLRPSVGSATGDDSLGFLEARDFGGIWLLDALWKR
                     LGIHRAVEGLLDDRAYTSPVERLMFAMVSSRILSPGSKLSIEHWVEKNAYIPGLPSVD
                     VHQLYRAMDLLVDSSEQFQHAIFQQVAKTIDLDVDLLFLDTTNTFFEIDYEDLGEEGL
                     RKRGQSKDNHPELPLISIAFAVTRGGIPVRCWVFPGNTSDQNIVEEVKRDLGTWNLGN
                     IIMVEDAGFNSSDNRRILRRCAGHYIIGEKLRTGSSGVAVEALHRKGRYKTLKNGLSI
                     KDVITDEGTAAMRRYIVVRNPESAARDKKIRDDIVAVTRQKLDELTQLSGEAHSKHAC
                     MLRSHSAYGRYIRQDEHGRLFLNTEKIASEELLDGKFL"
BASE COUNT         1520 a         1347 c         1403 g         1355 t
ORIGIN      
        1 cctcaagtat ggcaagattc ctgccttctg tcatgtcacc catactattc ccggatgccc
       61 ctaagtaaag aatgtttacg aagcctacga caagattatg acgtttgatc cttacaatat
      121 cgaggatttt ctcaccgcgc ataatatcat gacgaatgta gccttgcaaa atggaatccg
      181 atctttgcct ggataccaat ggaatcagtg ctgtacgaaa accggctaca ctattacgag
      241 gcgattgagc aggcaagaaa ggtggacgat tctggagctt tcattgaatt cacgctctct
      301 gcgattctcg ataccatcac gccgcaagtc gagcaccaag ttaagcacgc agttgagcac
      361 caagtcgagc tgagtgatat tcaatatgca gttctcaaat cgcttgagta caagagtctt
      421 tcgcgcaaag aaatctttgc cgcaatccga cggaatggcg attcacgctc cttcaaacgc
      481 atcgtcgaac tgctcattgt caccggccta gtcgaaatga ccgcaccaga caaaccaaac
      541 agtcgccttc aaaaatatcg gctcacccac caaggcaaaa tggcactgaa agagaacatg
      601 ccataaaact acaattcaga catcaagcat tccaggccga ggaagcaaac gccgtctgcg
      661 atgtgttcgc aggccaaccc taccacacgc cgacttatat gatagatccc ggtgtcgagc
      721 tcaacatgga gcccgggcag ctcgctctcc agcacgattg gcacttctta ggttttagca
      781 acgcaccttt ttcagtatcc gacgaggaag tactagacca tatccgtgcc attcaacgcg
      841 ctggacaaat caagccctcc gagaaactgg aaggccacta taacctgacc atcgaaatgg
      901 aaaccggtgt tggtaaaacc tacacctaca ttaagacgat gtacgagttc aatagccagc
      961 cttttaacgc acgcggcgct ggctatttct ctatcgataa gagaagtggg cgcatgatcg
     1021 atagtacctt gggcaataga agagagcgca cctctgatga tgccgatgcc tacgacctta
     1081 tcatgaaaga caaggaacgc ctcctcgacc ggcgtgagcg tatggacgaa aacctgattg
     1141 ccagggaaga aatccatact gtcaacgtgc taactgttgt cgccaacgaa agctacgaga
     1201 gctttgccaa aggtctgcag agcgaaattg ccgaggtagt tgctgaccgt ccacgcaagg
     1261 tcgaagcaaa gctgttcacc gatgacttcg gtgaggatac cgcccttgtt atctacgaaa
     1321 gccttatcga atatggttat gttaaacgcg gcgaattaac cgagaaattc tttgaagaca
     1381 agaaaaacga caagatcgaa ttaccgcagg aagccgcgaa ccgtactgcc gatgttatca
     1441 aggtgctgga ttctgtctac gatcccaatg cgatcaagcc agagaatgct cgcagcaaca
     1501 ctgtgcaagt gacgctcgac gaatccaagt tcaatagtcg cgctttccaa gagttgtggt
     1561 cgcgtatcaa tcagaagagt tactatatcg tcaattttga tgaaaaggaa cttgtgagca
     1621 aggctattgc agaacttaac gcgaagctgc gtgtttcaaa ggtctatttc aaggtcgaaa
     1681 aaggcgagca ggtaaggacg atcgaatcca agacgcaact agtagaaggt gctgcctttg
     1741 tacgcgcaga agtagcgcgg acgccagcca tgccctacac ggttatgaag gcgagcaatt
     1801 cggtccgtta tgaccttgtc ggcaaataca gcccggactg ggcaattgca ttttacgaag
     1861 gcaccgtaaa gcacatctat tttgtcgcgg aaaccaaagg cgacatggcc acgttggaac
     1921 tccgtgaaat cgaaacaaca aaggctcatt gtgcccgcga gcatttccgc gccatcagcg
     1981 gcgaaaatgt aaagtacgag gtggtcgata gctacgataa gctttgggaa attgtgagca
     2041 aatagaggct cctggcaagc gaggagttgt tgggaaaggt cctgaggatt atagctcggg
     2101 agtatcgatc cttaatatcg cttggaagca tcgcttgaca cagtaaagcg gcacactaca
     2161 cttgatatca ttggaagcaa gaatttattg acaccatgtc cagtaaacca tgtgtattca
     2221 tcctggcaga aggagcaatt caatggaact gaaagatatc agcaaagtat tgattgttgg
     2281 tgcagggacg atgggccaac agattgcctt ccaatgtgct gctcatggct acggagtcac
     2341 tatctgcgac gttaatgcgt ccgccttggc agtgggtctg catcggctct ccgcctacgc
     2401 cgacgagctc gtggccggcg gctacatgga taggcaaact gccgaaaaag ccgttacgca
     2461 aattgctgcc acgaccgacg agacggatgc ggcccacgaa gcggacctgc tcagcgaatc
     2521 gattccggaa gatcctgccc tcaagagaaa ggtatttgcc cgattcaacg agttgtgccc
     2581 cagcagaacg atttttacca ccaatacatc catgctgatt ccgtcggtta tcgccgaatc
     2641 caccggccgg cccgagcgat tcctcgcgtt tcattttcac caacccgtct gggtcgggaa
     2701 tgtggccgac attatgcccc atcccggaac cgcaacggaa attgtaaagc tggtccgcga
     2761 ttttgcccgc tccatcaatc agatacccat ggtgctccgc aaggaaaatc acgggtatgt
     2821 ttttaacgcc atgtacggct ctctgaattg tgctgcgatt gccttggtcg ccaatggcgt
     2881 cgcgtcggtt caagatgtgg atcgcgcctg gatgggaatt atgaaaatgc ccatcggacc
     2941 ccttggcatg ctggataatg tcggcctgga tacggtgttg cacatctcgg aaacatcgga
     3001 cgtcgactgc aaggacgagc agaaccgcac aaacattgaa ttcttgaaaa aggagtacat
     3061 tgacaaaggt tggctgggca caaaaactgg ccgaggattt tatacctatc ccaaccctgc
     3121 ataccaggat ccagattttc tgctggtcga tgagaactga acgaaatcat attgttgatt
     3181 ctgagttatc ttgttactct tattttgtca ttctgttctg tcattaggtt ccggatcttt
     3241 gtttctttgt tcccaagctc caatcgacca tctatccaaa gatcactgca cgtgccgctg
     3301 ccagcagttt tctagaccat aacaaatgca aggtgctggg gtgaccattc tgtctgacca
     3361 cttcacctca actgcgcgca caggcttttg gttgagtgtg tccatttcta cgagatcgac
     3421 ttcgagatct ttgccttcct tccagcgcgc acaggagaga gaactgtgca ggccttgtgc
     3481 ctgcagccat tgcgccgata tcgatgtctc tgcaaggttc ccaacggata cgagtcgacc
     3541 agggacttca gatgaacgct ctaatcctta tagatacggc tatgtttttt cctcatcgtc
     3601 tatgttgcgg ccttcgtacc gccacggact atctaacgaa aagcgccgca gtacttcttg
     3661 tgagggtatt tcaagcgatg ctcttatagt gtagtttcct ggtacttgtt ttgtcataat
     3721 acctggattc ccgcaccttt ataccaatga aggagagagt gcgagtgctt cgagtggatc
     3781 cttaatatca ttcgagatga gcgcaaagta gccgtagttc ttctctgcta agtcgattgc
     3841 ctcttgcctg ggtatcatga caagccctcg gaaaggatgt gaaggaatat tctcaccata
     3901 cggatgatgg tgtgtttttg cccagcgagg aaaacgctgt aaaggatttt tgtcttcgag
     3961 aataagataa taagcaagag aaatcagttg cttatagatg gcagggaatc actttgacac
     4021 ttgacatgat ggtgctctag aggaggaagg cgaagacctt ctttgtggct ctattgcaag
     4081 ctgtattgga gagggtcaga atagcttttt ggaaagcgaa ttgggcagtc tgtggctgtc
     4141 tgaaagacta aaaagtgagt tcttcagccg gcgttgtaca gtgtttagcc aataaatgac
     4201 caagcgatcg gggcaaattc ttatagtaaa agaaataaga gattgtacgg gtcgtacgaa
     4261 acaattaaaa gtgtaatact agaaatatat aaaagacttc acaaataaag attatagtac
     4321 tatactatgt agtaccatga atatccgaac gactacatcg gtccagggtt ccaaaactta
     4381 ccactatgtg cagcttgttc agagtgttag agatgaaaag accggaaaac ccaaaactat
     4441 cgtcctctac aattttggaa aacctgaaga tgttgacctc gacgccgtca gaaagcttat
     4501 cgccgcgctg tcaacgctcc ttcctcacgg ggaattggta ctacgcccct cggtagggag
     4561 cgccacagga gatgactctc ttggctttct tgaagctcgt gattttggtg gcatctggct
     4621 tcttgacgca ttgtggaaac gcctcgggat acacagggct gtcgaaggcc ttctcgacga
     4681 tcgagcctat acatcacccg tggaacggct catgtttgcc atggtgtcat cccgaatcct
     4741 ttccccaggc tcaaagctct caattgaaca ttgggtagaa aaaaatgcct atattccagg
     4801 gctgcctagc gtggacgtac accagctcta ccgtgccatg gatttgctcg ttgactcatc
     4861 ggagcagttt cagcatgcca ttttccagca agtagcaaag acgatcgatc tcgatgttga
     4921 tctcttgttt ttagatacta cgaatacctt ctttgaaatc gattatgagg atctcggcga
     4981 agaaggattg cggaagcgag gacagagcaa ggacaatcat cctgagttac cgctcatctc
     5041 cattgccttt gcggtaaccc gtgggggaat accggtacgg tgctgggtgt tcccggggaa
     5101 taccagtgac cagaatattg tggaagaagt aaagcgggat cttgggactt ggaatcttgg
     5161 gaacatcatc atggtggaag atgccggatt caattctagc gataaccgaa ggattctgcg
     5221 gcgatgtgca gggcactaca tcattggcga gaagctgcga acggggagtt ctggcgtcgc
     5281 cgtggaagcg cttcaccgca agggtcgcta taaaacgctt aaaaatggcc tttcgatcaa
     5341 ggacgtgatc acggacgaag gtacagctgc catgcgtcga tatatcgtgg tcaggaatcc
     5401 tgaaagcgcg gctcgagaca agaagatccg cgatgatatt gtcgctgtta cgcggcaaaa
     5461 gctcgatgag cttacgcaac tatcgggcga agctcatagt aagcacgcct gtatgctgag
     5521 aagccatagc gcctatggca gatatattcg acaggacgaa cacggaaggc tctttttgaa
     5581 taccgagaag atcgcgtcgg aagagctctt ggatggcaag tttct
//
LOCUS       DAOOWY010000112         5588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_14652_length_5588_cov_29.254473, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000112 DAOOWY010000000
VERSION     DAOOWY010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14652_length_5588_cov_29.254473"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PK105_07580"
     CDS             <1..959
                     /locus_tag="PK105_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61612.1"
                     /translation="MTALANSAGTVIREVSVKGIIRFNQSNEQLSGITLVDADTMRDL
                     LGFASLRDGAPQLSEHEAAFLTDFDPDSLFDNLFASQTILTEGEEQPEIVSGVSSEDM
                     LENAGLGTIQVSSSTTVTAEIVPAWQFLLVRAKKGANTSKLLDELQAFSEQLGDGDRV
                     QDWIAGAGKVARTAVTIRLVFDLLVAIIAVVVVMIMMNVLVISVNERLHEIGTLRAIG
                     AKRHVIRSMILYETTFMALIAGAAGLIIGFILLLIMGKVGIRAPNLFFEALFGGEILK
                     PVISIGAAIRAFLWILAMALAACWYPTRLAVRIEPVVAMRGE"
     gene            962..2269
                     /locus_tag="PK105_07585"
     CDS             962..2269
                     /locus_tag="PK105_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU61613.1"
                     /translation="MHLSNLKLHLKLAARNVRRQPNRTIALGGAVAFSALVMTLISGF
                     VSGMDMAIQDNVTLYSGGHILISGYTPSWSGRLQNRFIDDEIAKTVESAVTDIRTISP
                     LAQARATIVFGTREIQLTLRGIDWTKDELYHDSLLLSSGDWKTLNEQDRSMLMSSQTA
                     DRFGLSIGDQVVVRLTTASGQQNVTDYAVSAIYDDAASGGMTTAFVSFGNLIADLNMK
                     PNEQQQIAIFLKDSTNAERAAKAIAEALTEKGYSIASGTSGSEQATQRRMNSGKVVYS
                     ISTVQELAGEIGSALGSIRWIGYAVFILMLLVSATGIANSYQMVLLERTKEIGMLRCI
                     GFKKKDVFASFISEGILLAGAAAIAGILCALPIGLGISFIPFDPHGDFGAALVKGHLR
                     FVPTLGQLLIILVFIMIVAVVAVFIPARKAAEVVPVEALRITA"
     gene            2311..3228
                     /locus_tag="PK105_07590"
     CDS             2311..3228
                     /locus_tag="PK105_07590"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOU61614.1"
                     /translation="MLRFYEKKTKYFPSKSIRDISLVILGLVLVLCAQAQGSPPPGGP
                     KGGGGPMAGIGLPLGFLEEDKNYKFPDDITKYTAKDFLMLVRRTDIRSSFYNSDMSAT
                     ITTVNVSPTRGTFVTKEQIFRRDKDDAFLMLTLEPESRKGQGMLRVNNNMWRYDPTSR
                     KFTHTTLKDTYENTTVTNNDFRRWQRSIDYSVEKLSTGTLGNYKVIIGELRANNDEVP
                     FPYIKMYVEADRKIVLKVEEYSLSMKLLRTAYYTQYVQIGDSFVPTVQIFQDGLVPEK
                     RSQVTYTNISTKPLPDYYFTKEYLERVSQ"
     gene            3239..4864
                     /locus_tag="PK105_07595"
     CDS             3239..4864
                     /locus_tag="PK105_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61615.1"
                     /translation="MKTRIIKSLLFLIMTTLFLFSKSSIWAQSSSSSSNLKPPEVIDE
                     NELFGGPKDIVTVIDPNVASAGTVQLVEDTKTYPVFLISGSAGAGLYGNYTPLGGSSS
                     DKQTLYGAVDISDLEFDYLPAKNLHFSLSTNLLAVPNQILAASATAYADLRQSDLVRL
                     YVSGSFNYDPSSYLSTNKEYESPTLSLDELFVDTHIEETLFFRLGKQRISWGVGNWFK
                     PSDVLSLSAIDPDDPTAAREGPFAFKVDVPMKLNHLMVYIVPPISENNAASSSAASKY
                     DLVVNGWELSFGAYARADMLARPRAIFSFTGAIGSFDVYGENVLLYGSDRIYARESST
                     PGTYEAYSIENKPFFQSTLGVKYSKSYSGGLSYSIHVQGYYNGMGYADSSIMQNAAAT
                     AAITEAIEDDYPTAYRYSDSSTDAGMWYLAGSASVSGRFGEGNSLTRVTGSVYALANF
                     SDGSLRFNPQLKVQVGDEGGKVSFTISDLTSIGDVGSEYAKNGNITTPALTVSLLNSQ
                     VSLQASVPIKLNADYSVKKVQTKFSILWNAIEF"
     gene            4933..>5588
                     /locus_tag="PK105_07600"
     CDS             4933..>5588
                     /locus_tag="PK105_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU61616.1"
                     /translation="MHGRILIVEDTKELAELYKLYLQNEGFECKLAFDAESALPIAQK
                     EEWDLIVLDLNLPGMDGFQFLEQLRKTKSMPVMILTAREADEDVIFGLGVGADDFVTK
                     PCPPKVLAARIRAHMRRTATLQMRQNGSIVRFGPYELDIEGMYLTKNAEPVSLSAREF
                     NILKELVSNPGRPFTPEDLYTRVWGQEYGDISAIGVYIQRLRKKLEDDPANPYYIQTQ
                     "
BASE COUNT         1474 a         1250 c         1405 g         1459 t
ORIGIN      
        1 tcatgactgc gctggccaat tcagcgggca ctgtaatacg cgaagtcagc gtgaagggca
       61 tcataagatt caatcaaagc aatgagcagc tttcgggtat cactcttgtc gacgcggata
      121 caatgcgcga ccttttgggt tttgcgagct tgcgcgatgg agctcctcag ttgagtgagc
      181 acgaagcagc cttcttaaca gacttcgatc ccgatagcct attcgataat ctattcgcaa
      241 gccagacaat tctgacagag ggcgaagaac aacccgagat tgtttctggg gtttcttcag
      301 aggatatgct ggaaaacgca gggttaggaa caattcaagt ctcgagctcc acaaccgtga
      361 ctgcagagat agtgcccgcg tggcaatttc ttcttgtgcg cgcaaaaaag ggcgctaaca
      421 cttccaagct tttggacgag ctccaggcct tttccgaaca gcttggagat ggcgaccgtg
      481 tgcaagattg gattgcagga gcgggaaagg tcgcgaggac ggcagtaacg attcgacttg
      541 tattcgatct attggtggcg atcattgcag tcgtcgtagt tatgataatg atgaatgtcc
      601 ttgtcatatc cgtcaatgaa cgcctccacg agatcggcac gcttagagcc ataggagcca
      661 agcggcatgt aattcgcagc atgattctgt atgagaccac attcatggcg ctgatcgctg
      721 gtgcagctgg ccttattata ggatttatct tgcttcttat tatgggaaaa gtcggcataa
      781 gagcgccaaa tctctttttc gaagctcttt ttggaggaga aatcttgaag ccagttatat
      841 cgataggagc ggcaattcgc gcgtttcttt ggattctggc tatggccttg gctgcttgct
      901 ggtatccgac aaggctagcg gtgcgaatcg agccagttgt cgcaatgaga ggagaatgaa
      961 tatgcatctt tcaaatctta agctccattt gaagcttgcg gctcgaaacg ttcggagaca
     1021 gcccaatagg acgattgccc ttgggggtgc ggtggccttt agtgcgctag ttatgactct
     1081 tatatctggg tttgtgagcg ggatggatat ggcaattcag gacaatgtga ctctctattc
     1141 aggggggcac atattgattt cgggctatac tcctagctgg agcggcaggc ttcagaatcg
     1201 atttatcgat gatgagattg caaaaactgt agaatctgct gttacagata tcaggacgat
     1261 ttctccactt gctcaggcaa gagctacgat agttttcgga actcgagaaa tacagcttac
     1321 gctccgtggt atcgattgga caaaggatga gctatatcac gattctctcc tcttatcgag
     1381 cggagattgg aaaacgctca acgagcaaga tagaagcatg ctcatgagct ctcagactgc
     1441 agatcgattc ggcctttcga ttggagatca ggtggtcgtg aggctgacaa cggcatccgg
     1501 tcagcagaat gtgacggatt atgctgtttc tgcgatctat gacgatgctg catcgggggg
     1561 gatgacaact gcttttgttt cttttgggaa tctaatagcc gatctcaata tgaaaccaaa
     1621 tgagcagcag caaatagcga tttttctcaa ggacagcacc aatgcagaac gcgcggccaa
     1681 agccattgcc gaagcgctta ccgaaaaagg atatagcatt gcctctggaa cctctggaag
     1741 cgagcaggca actcaacgca ggatgaattc aggaaaggta gtctactcca tctctacagt
     1801 acaagagctc gcgggagaaa taggctccgc tcttggctcc atacgctgga ttgggtatgc
     1861 ggtgttcatt cttatgctgc ttgtaagcgc aacggggatt gcaaattcat atcagatggt
     1921 gctcctcgag cggactaagg aaattggtat gctccgatgc attggtttca agaaaaaaga
     1981 tgtctttgca tcttttatat ccgaaggaat tttacttgcg ggagcggcgg cgattgcagg
     2041 aattctatgc gctctgccaa taggcctggg tattagcttt attcccttcg atccccatgg
     2101 agattttgga gcagccctgg tgaaaggaca tttgcgtttc gtcccgaccc tgggtcagct
     2161 tcttataatc ttggtattta tcatgattgt ggctgttgtt gcagtcttta taccagctcg
     2221 aaaagcagct gaagtagtgc ccgtcgaggc acttaggatt acagcgtaat gcttgcaata
     2281 ttgcggttgc gctaaaatga ggagcgattg atgttgcgat tctacgagaa aaagacgaag
     2341 tacttcccat caaaaagcat tagggatata agccttgtga tcttagggct tgttcttgtt
     2401 ttgtgtgcgc aggctcaggg cagcccacct ccaggcggac cgaaaggtgg cggaggaccc
     2461 atggctggta ttgggctgcc gcttggtttt ttagaagaag ataagaacta taaatttcct
     2521 gatgatataa caaaatatac ggcgaaagac tttcttatgc tcgttcgcag gaccgatatc
     2581 cgttcaagct tttacaacag cgacatgagc gctaccatca cgaccgtaaa cgtgagcccg
     2641 actcggggta cctttgtaac caaggaacag atctttaggc gagataaaga tgatgcattc
     2701 ttgatgctca ccctcgagcc cgaatcccgt aaggggcaag gtatgcttcg agtgaacaac
     2761 aatatgtggc gctacgatcc cacaagccga aagttcactc acacaaccct taaagacacc
     2821 tatgagaaca cgacagtaac aaataacgat ttccgtcgct ggcagcgctc gattgattat
     2881 tctgtggaga agctctcaac gggtactctt ggaaactaca aagtgataat cggagaacta
     2941 agggctaaca atgacgaagt tccattccct tacatcaaga tgtatgtgga agcagaccga
     3001 aagatagtgc ttaaggttga ggaatacagc ctctctatga aacttctccg caccgcctac
     3061 tacactcagt atgtgcaaat aggagattcc tttgtgccta cggtccagat ttttcaggat
     3121 ggacttgtgc ccgaaaagcg ctctcaggtt acctatacca atatttccac taagccgctc
     3181 cccgactatt actttaccaa ggaatacctc gagcgggtga gccagtagga gtagaggaat
     3241 gaaaacaaga ataattaaga gcttgttatt ccttataatg acgactctct ttctttttag
     3301 caaatcaagt atctgggcgc aatcctcaag ctcttcatcc aacttgaagc cgcctgaagt
     3361 gatagatgag aacgaactct tcggtggccc caaggatatt gttacggtga tagatccaaa
     3421 tgtcgcttcg gcgggaactg tgcagcttgt ggaagacacc aagacttatc cagttttctt
     3481 gatcagtgga tcagctggag ctggtctata tggcaactat actccacttg gaggcagttc
     3541 ctcggataaa cagacgctct atggggcggt ggatatttcc gatctcgaat tcgactatct
     3601 tccagctaag aatctgcatt tttccttatc caccaatctt ctagctgtgc ccaaccaaat
     3661 tcttgctgcg tctgccactg cgtatgcaga tttaaggcag agcgaccttg tgcggctcta
     3721 tgtctcgggt tccttcaact acgatccatc gtcgtattta tcgacaaaca aagaatatga
     3781 gtctcctacc ttgagcctgg atgaactctt tgttgataca catatagaag agacactctt
     3841 ctttagattg ggcaagcagc gtataagttg gggagttggg aattggttca aaccctcgga
     3901 tgttctgagc ctatcggcta ttgatccaga tgatccaacc gcagcgcggg aggggccctt
     3961 tgcattcaag gtcgatgtgc cgatgaagct aaatcacctt atggtatata ttgtgccgcc
     4021 aatttcagaa aacaacgcgg cttcgtcttc tgctgcgagc aaatacgacc tcgttgtaaa
     4081 tggatgggag ctcagttttg gtgcatatgc ccgggccgat atgctggcaa gacctagagc
     4141 gatcttcagc tttactggcg cgataggttc ttttgacgta tatggagaaa acgtgctctt
     4201 gtatggttcc gatcgaatct atgctagaga gtcttcgaca cctgggacct acgaagctta
     4261 tagcatagag aacaagcctt tctttcaatc tacattgggc gtcaaatact cgaagagcta
     4321 ttcaggcggc ctatcgtatt cgatacatgt tcagggctac tataatggta tgggttatgc
     4381 agattcgagc ataatgcaga atgccgcagc cacagcagcg ataacagagg cgatagaaga
     4441 tgattatcct actgcttata ggtatagcga tagttcaaca gacgcgggca tgtggtatct
     4501 cgctggatcc gcctcggtat cggggcgctt cggagaaggc aacagtctca cccgtgtaac
     4561 aggctctgtc tatgctcttg ccaatttttc cgatggttcg ctgcgcttta atccgcaact
     4621 taaggtacaa gtaggagatg aaggtggaaa ggtgagcttt acgatttctg atttgacttc
     4681 tattggcgat gttggttctg agtatgctaa aaatggcaat ataacaactc cagctctcac
     4741 cgtaagcctt ctaaacagcc aagtatcgct ccaagcttct gtgcctataa agctcaatgc
     4801 ggattattcg gtcaagaagg tccagacgaa gttcagcatt ctctggaatg caatagagtt
     4861 ttgatgccaa caagtggaga atcaatgcaa gtagcctggc agcgttcacc gggcgcttgc
     4921 gaaggagggg gcatgcatgg aagaatccta atcgtcgaag ataccaaaga gcttgccgag
     4981 ctctataagc tctatcttca gaatgaaggc tttgaatgca agctcgcatt cgacgcagag
     5041 agtgctcttc ccattgccca aaaagaggag tgggacctca tagtgctcga tctaaacctt
     5101 cctggcatgg atggctttca atttcttgag caactcagaa agacaaagtc catgcctgta
     5161 atgattctca ccgcaagaga ggctgatgaa gatgtaattt ttgggcttgg cgtaggagca
     5221 gatgacttcg tgactaaacc ctgtcctcca aaggtccttg ctgcacgcat ccgagctcac
     5281 atgaggcgta cggctaccct tcaaatgcga caaaatggga gtatcgtaag atttgggcct
     5341 tatgagctag atatcgaggg aatgtatcta actaaaaatg ccgagccagt cagcctttct
     5401 gcgcgcgaat tcaacatcct aaaagaactt gtaagcaatc caggcaggcc atttactcct
     5461 gaagacctct atactcgcgt atggggtcag gaatatggtg atataagcgc catcggagtc
     5521 tatattcaga gattgcgcaa gaagcttgag gacgatccag caaatcctta ttatatccag
     5581 acccaata
//
LOCUS       DAOOWY010000113         5548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_14784_length_5548_cov_10.349536, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000113 DAOOWY010000000
VERSION     DAOOWY010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14784_length_5548_cov_10.349536"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..2343
                     /gene="ftsH"
                     /locus_tag="PK105_07605"
     CDS             304..2343
                     /gene="ftsH"
                     /locus_tag="PK105_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU61617.1"
                     /translation="MMPSKGKNNWKLDQQRKTDKNSNNRKKPGMPEGFQFRFSLGYVL
                     IALLAVSFFNYFLFKSDTTLVPYSAFKEKVRSGEIRRVEIDVNYYTGYTEPSPSEPSS
                     PLPPSAKPGVVYKTVPVSDPEFTSLLDEKGVVYSASPRGGSTILSLLLNWILPFAIMI
                     FLWRTIMSRFMGSNSNVLAFGQNKATVVAEGDIKTRFADVAGVDEAKAELVEVVDFLK
                     NPQKYTEIGGKIPKGVLLVGPPGTGKTLLARAVAGEANVPFFKMSGADFVEMFVGVGA
                     ARVRDLFKQAREKAPCIIFIDEIDALGKSRASGFIGGNDEREQTLNQLLVEMDGFDST
                     SGLIILAATNRPDVLDPALLRAGRFDRQVLVDKPDMKGREEILKIHTKNIKMDPSVDL
                     SKIARSTPGFAGADLANVVNEAALLAVRAGRKKVKEEDFQNAIEKVIAGLEKKNRMIN
                     PKEKQIVAVHETGHAITAALTPGADPVRKISIVPRGYGALGYTLQMPLEDRYLITEEE
                     LLGKIDVLLGGRAAEELVFQSISTGASNDIANATEIAKRMITDYGMSDKFKNVALTRR
                     SSGLISPQQASDPFATKEYSEETQRYIDETIASMINERYRHVVNLLTEKRDLLNRISS
                     VLLEKEVIEESEFAELLEGAGVSMPEAIKEPVAAAESVPLPGSTEKVFISPTPHE"
     gene            2661..3638
                     /locus_tag="PK105_07610"
     CDS             2661..3638
                     /locus_tag="PK105_07610"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOU61618.1"
                     /translation="MSIVVRSISAYIPSLRITNEELAMRMDTTDEWIRSHTGIGSRHI
                     APDGLQTSDMATEAAKSALDKANISVQELDYIILATATPDYLGFPATACIVQDKIGAY
                     GVASFDVAAGCTGFIYALNIASSMLEASHGRNALVIGAEGLSRFVNWEDRSTAVLFGD
                     GAGAAILSRIDESSRGCLSFILGAEGSGTKDLFLVQPERTKAFERQEPIIPFIYMDGK
                     KVYDFAVKSITVLIQRLLHKTAYKIEDFTWIVPHQANARIVQAAAKRLSIPLDKFYIN
                     IEEYANTSAASIPIALAEMCEKGLLKKNDLIMLVGFGAGLTYGAAVIRW"
     gene            3746..5026
                     /gene="fabF"
                     /locus_tag="PK105_07615"
     CDS             3746..5026
                     /gene="fabF"
                     /locus_tag="PK105_07615"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU61619.1"
                     /translation="MQNEVAVTGLGIISPIGNSIDEFWKNCVNGATGIGPITHFDASR
                     LESRIAAEVKNFDPSVWIDRKEARKMALFSQYAVAAAAQAWLDAGLPDFVQNETLENK
                     SDIDPNRIAVILGNGIGGLEVFTGSHAKMLQDGPDRIPPMTIPQMIANEAAANIAMHF
                     GVHGPVLTQVTACSSGTDALGEALDLIRAGRADIVIAGGTEAAITEFAVGSFCRLKAL
                     STSYNERPEVASRPFDRDRDGFVIGEGAGILILERLDYAKARNAKIRAIFAGYGASCD
                     AYHLTAPQPEGVFGVKAIEHALRDADLQSADIEYYNAHGTGTDLNDPMETRMLKKAFG
                     SSVQSLKISSTKSMTGHCIAAAGAIEAIACIKALETGMLPPTIHLDNPDIEQGCDLDY
                     IPNTAQHRKIKTAMSASLGFGGHNGIIIFKAVGA"
     gene            5119..5535
                     /gene="fabZ"
                     /locus_tag="PK105_07620"
     CDS             5119..5535
                     /gene="fabZ"
                     /locus_tag="PK105_07620"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOU61620.1"
                     /translation="MNVESYLPHRPPFLFVDDVHIKGDTILAQHTFRDDDWFFKGHFP
                     SFPIVPGVLLIESMAQAGGVGVKMMGIYPKSLFMFAKIKEARFRQPVRPGDTLIIKIK
                     NVRASSVYLHQKGIGKVGDKVAVEAEWISIASGVPE"
BASE COUNT         1541 a         1196 c         1342 g         1469 t
ORIGIN      
        1 gcactctgtc cccaatgggt tttacggcca taaatatgtc ctcctaataa atgctggatg
       61 atttgtggta gagaaccgag ttgaaatata aaaaaacaat cccttctggt caagcatgtc
      121 catctacttt tgaacagatc gagctaaaag ccacatgcct aagccacatt ccgaaatgtg
      181 aaatagaata ttcgccaaat aaaatgaaaa tcattatatt ttcagatagt gaacgtttct
      241 gcttacgaga gatatgcaaa tgctctataa ttaaaatagg ccatcgattt atttgaggag
      301 ataatgatgc catcgaaagg caagaataac tggaaactcg atcaacaaag gaaaactgac
      361 aagaattcaa ataatcggaa gaagcctgga atgccagaag gctttcagtt taggttttcc
      421 ttagggtatg tgctcatagc acttttggct gtctcttttt tcaattactt tctcttcaag
      481 agcgatacca ctcttgttcc ttattcggcc ttcaaggaaa aggttcgaag tggcgagatc
      541 cgtagagtgg aaattgatgt gaactattat accggttata cagagcccag tccttcggaa
      601 ccgtcttctc ctcttccacc atctgcaaaa ccaggtgttg tctataagac tgttccggtc
      661 agcgatcctg aattcacttc gctgcttgac gaaaaaggtg tagtatattc ggcgtctccg
      721 cgtggaggaa gcacgatact ctcgctcttg ctcaattgga tacttccatt tgcaataatg
      781 attttcttat ggcgcactat tatgagtagg tttatgggat ctaactcgaa tgtgctagcc
      841 tttggacaga ataaagccac agttgttgcg gaaggcgaca taaagacgag atttgcggat
      901 gtcgctggag tcgatgaagc taaggctgaa ttggtagaag ttgtcgactt tctcaaaaat
      961 ccacaaaaat atacagaaat cggcgggaag attcccaagg gtgtgctcct tgtagggcct
     1021 ccgggaacag gaaaaacctt gttagccagg gcagttgcgg gcgaggccaa tgtgcctttc
     1081 ttcaagatga gtggtgctga ttttgttgaa atgttcgttg gagtcggtgc cgcccgagtc
     1141 cgcgatctct ttaaacaagc cagagagaag gctccatgca taatttttat cgacgaaata
     1201 gacgcccttg gtaaatcgcg ggcaagcgga tttatagggg gcaacgatga gcgcgagcag
     1261 acacttaatc agctattagt cgagatggat ggttttgatt cgacaagtgg cctcatcatt
     1321 cttgcagcaa caaataggcc tgatgtcctg gatccagctc tcttaagagc cggaagattc
     1381 gatcgtcaag tattagtaga taagcccgac atgaaaggtc gggaggagat tcttaagatt
     1441 catacaaaga atatcaaaat ggatccttcg gtcgatctgt ccaaaatagc tcgcagtact
     1501 ccgggtttcg caggggccga tctcgcgaac gtcgtcaatg aggcggctct attagcggtt
     1561 cgtgccggcc gaaagaaagt caaagaggaa gatttccaaa acgctatcga gaaggttatt
     1621 gccggtctag aaaaaaagaa tcgtatgatt aatccaaagg aaaagcagat agtggcagtg
     1681 catgagacag gccatgccat aacggccgct ttgactccag gtgccgatcc agttaggaaa
     1741 atctccattg tgcctcgagg ctatggagca cttggttaca ccctccagat gcctctcgaa
     1801 gatcgttatc tgatcaccga agaagaatta ctgggcaaga tcgatgtgct tctcggcgga
     1861 cgcgctgcgg aagagctggt attccagtcc atttccaccg gagcttccaa tgatattgcc
     1921 aatgccacgg agatagccaa gagaatgatc accgactatg gcatgtccga caaattcaag
     1981 aatgtggcct tgacgcgaag atcgtcgggg ctaataagcc ctcaacaagc ttccgatccc
     2041 tttgcaacaa aagagtatag cgaggaaaca cagcgataca tcgatgagac tatagcttcg
     2101 atgataaacg agcgatatcg gcatgttgtc aatctgctta cagaaaaaag ggatttgttg
     2161 aatcgcatat cctctgtatt gctcgaaaaa gaagtcatcg aagagtccga attcgctgag
     2221 cttcttgaag gagctggcgt ttctatgcca gaggcaataa aagagcccgt ggctgcagcg
     2281 gagtctgttc ctctgcctgg atcaacagag aaggttttca tttctccgac tccacatgaa
     2341 taagattcca ggagatttgg ctcaattcta agtcgatgag tcttgctggt tctctattcg
     2401 gctctcttcc ctactctatt ctatgtccag ctctcatcat tatttctttt ctagatcttt
     2461 tcatggctcc gtttctattc tactcccagt tctcttccgg gctccttgat taagcgactc
     2521 aaaatatact ctaaccaatc ctgaaaattg gcatataata aaatatattg acatttttac
     2581 tcttattgtc atattgacaa tataatccca ttgtgattga ttaagggcat ctgtcgtagt
     2641 gaataaggag atgtcgctgt atgtccattg ttgtcagatc aatttcagcc tatataccgt
     2701 cccttcgcat cactaacgaa gagcttgcaa tgcgcatgga tacgacggac gagtggatac
     2761 gctctcatac cggtatcggt tctcggcata ttgcgcctga tgggcttcaa acaagcgata
     2821 tggcaacgga agccgcaaag agtgctttag ataaggcgaa tattagcgtg caggagttgg
     2881 attatatcat tcttgcaaca gcaacacccg attatcttgg ctttcctgct acggcctgta
     2941 tcgttcagga taagatcggt gcttatgggg tcgcttcatt cgatgtagcc gccggttgta
     3001 caggtttcat ctatgcgctc aacatagcat caagcatgct cgaagcatcg catggtagaa
     3061 atgctctggt tataggtgcc gaaggcttga gtcgatttgt caactgggaa gatcgctcga
     3121 cagcagtctt gtttggcgat ggggctggtg ctgcgatact ctcgcggatc gatgaatcct
     3181 cccgcggatg cctttctttt attctcgggg ccgaaggaag tggaacaaaa gatctatttc
     3241 ttgttcagcc cgaaagaact aaggcctttg agcgccagga acccatcata ccattcatct
     3301 atatggacgg gaaaaaggtc tatgattttg cggtcaaatc gataactgtc ctaatccaaa
     3361 ggcttcttca taaaaccgct tataaaatcg aagattttac atggattgtt ccacatcagg
     3421 caaacgcgcg catcgttcaa gccgctgcca aacgcttatc gattcctttg gataaattct
     3481 atattaatat cgaagaatat gctaacacct ctgcggcaag catacctatc gctctcgcag
     3541 aaatgtgcga aaagggactc ttgaaaaaaa atgatctcat aatgcttgtt ggctttggtg
     3601 cagggctcac ttatggagcg gcggttattc gatggtgagg ctcttgtctt gcggaatatg
     3661 gctatgatat cgcataatat tggcaaatct ttacttgtat atgaagactt ttgcagcaat
     3721 atggcaatgc aaggagaagg caagaatgca aaatgaagta gcagtgacgg gtcttggaat
     3781 cataagccct atagggaatt cgattgacga gttttggaaa aactgtgtca acggagcaac
     3841 cggcattggt cctatcacac acttcgatgc atcgaggctg gaaagcagga tagccgctga
     3901 agtaaagaat tttgatccat ccgtatggat cgatcgaaaa gaagcacgga aaatggcgct
     3961 tttttctcag tatgcggtcg ccgctgcggc tcaggcttgg ctagatgccg ggctgcctga
     4021 ctttgtccag aatgagactt tggagaataa atccgatatc gaccccaatc gcattgcggt
     4081 catcttgggc aatgggatag gaggcctaga agtatttacg ggatctcatg ccaaaatgct
     4141 gcaagatggg ccggatcgga tacctccaat gaccattccc caaatgattg cgaatgaagc
     4201 tgccgcaaat atagccatgc attttggtgt tcatggtcct gttttgacac aggttacggc
     4261 ttgttcctca ggcaccgatg cgcttggcga ggcactggat ctcattcgtg ccggacgggc
     4321 cgatatcgta attgcaggag gaacagaagc agccatcact gaatttgcgg ttggctcctt
     4381 ttgcaggcta aaggcgctgt cgacttccta taacgagcga ccggaggtag catccaggcc
     4441 tttcgaccgc gatagggatg gttttgtcat aggcgaagga gcaggtattt taattctcga
     4501 gagactcgat tacgccaagg cccgaaacgc gaaaatccga gcaatctttg cgggttatgg
     4561 cgctagttgc gatgcttatc atctaactgc tcctcagccg gaaggtgttt ttggagtaaa
     4621 agccatagaa catgccctaa gagatgctga tttgcaatca gcagatatcg aatactacaa
     4681 cgcacatggc acaggcaccg acctcaatga ccctatggaa accagaatgc taaagaaagc
     4741 ctttggttcc tctgtacaat cgctgaagat cagctcgaca aagagcatga cggggcattg
     4801 tatcgcggct gctggagcga tcgaagctat tgcatgtata aaagcgctag aaacaggcat
     4861 gcttcctcct acgattcact tagataatcc agacatagag cagggctgcg acctggacta
     4921 tattccaaat actgctcagc atcgcaagat taaaaccgcg atgtcggcta gccttggttt
     4981 tggcggacac aatggaatta tcatattcaa ggcagttgga gcataaaacc tgttgcagga
     5041 ttcaaatcag atggctaaga ttgcttagtt tagcaaagga atcagtttgc agaccagata
     5101 aggagagatt gaacggtgat gaatgttgaa tcttatcttc ctcatagacc tccatttctg
     5161 tttgtcgatg atgttcatat caaaggcgat actatactgg ctcaacatac attccgagac
     5221 gatgattggt tttttaaagg gcattttcct tcctttccta ttgtgcccgg agtcttgttg
     5281 atagagagta tggcccaagc gggcggagta ggtgtaaaaa tgatgggcat ctatcccaag
     5341 agcctcttta tgtttgcaaa gataaaagaa gcgcgtttca gacagcccgt gcgccccggt
     5401 gatactctga tcatcaaaat aaagaatgtg cgtgcctctt ctgtctatct tcatcaaaaa
     5461 ggcataggta aggtaggcga taaggtagct gtggaagccg aatggataag catagcttca
     5521 ggagtaccgg aatgataata aagccaat
//
LOCUS       DAOOWY010000114         5503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_14922_length_5503_cov_10.994493, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000114 DAOOWY010000000
VERSION     DAOOWY010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14922_length_5503_cov_10.994493"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PK105_07625"
     CDS             complement(<1..355)
                     /locus_tag="PK105_07625"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF020140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU61621.1"
                     /translation="MNRNSVLRRPFKFKYFNATLYLIAANVIIFALQYIWPLLTYVLA
                     LNPAAVASGWVWQLFSYMFAHANLTHLIVNMLGLYFFGRPVEHSLGSYEFLLYYLGSG
                     LFAGIASFAIYLLSGA"
     gene            complement(536..1012)
                     /locus_tag="PK105_07630"
     CDS             complement(536..1012)
                     /locus_tag="PK105_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61622.1"
                     /translation="MKKFAMVALFAVVSLGMVFAAVDTNTGNVTIQGTVSQVFQLTVP
                     GNYTGSISYNSSTHWDLSKMKVISNVNNWILTVSSQNNGNLVDTATSGKIAYKVTVGS
                     LFNKQQPTTAGIRSSAQTMTPKDGTEYAFAIDIDAPSGMYQAGTYTDIITLTVSGQ"
     gene            complement(1377..2858)
                     /locus_tag="PK105_07635"
     CDS             complement(1377..2858)
                     /locus_tag="PK105_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU61623.1"
                     /translation="MSIVLLSLLFCVSAIFFFVMLYKSANRSESELLGSVSKRIAQQI
                     SESLQEMLTVGEGLRELIIAGSGRNYASLGPLADEALARLPYIDSITIAPGAIVRYFF
                     PESRASASIGHDLLDNPDRMRALVEAVRKKSAVLQGPELSAEGKNLAFLRIPVIEGSE
                     LWGFVSIAFDADKLLSDLDLVSQFPGLSIAVASSDMEKGDKELFWGEESALSGYSAGL
                     KIGSEDFSWTIYVASLYPYRQVALWGAGLLILVLLSCGLAILAGSLEKEGKAPIGSKE
                     ALITIKPFVPKTEPLQESSIEVIKKAEILQEPLAENLVSASLGRPIKETVEGPAQEKI
                     ILRDQEIENEAENKESAAPKEKIASARSVLVVDDSEVNRDLLLRMLTLKGYEARAVSS
                     AEAALESLRVKSFDIMLIDCVMPEMDGYALAQKIRAEDISQQKGLQSALPRPVLIAMS
                     PRHDQEEAEKCAKAGFDSLLVKPFTMTALDQQIRLIFDDKRIG"
     gene            complement(2977..3480)
                     /locus_tag="PK105_07640"
     CDS             complement(2977..3480)
                     /locus_tag="PK105_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61624.1"
                     /translation="MKNKVFLSGLVLLLIAIPVFASGSSTINLDATVSQILSITKCSG
                     FTETLDTTNAGAINGVCLGAVGIKANVQNWEIQIKSDNTWKMVKAAGQPGSDSGYPYE
                     FRFGDTGETIVNSTSEPDGLYTIQKNAPLITEGYFNIYVFYQTAEALGILAGSYTDTI
                     TITLSAT"
     gene            complement(4381..4899)
                     /locus_tag="PK105_07645"
     CDS             complement(4381..4899)
                     /locus_tag="PK105_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61625.1"
                     /translation="MKKWVVVVFALFMILKLNAQASIGITVVVAPPPGASITMDSYIW
                     DILFANDGVTTYQNDNQGTMTIRSSNRKNFRVDFSSQNLGSLKQGTDYYVPYYIKATR
                     ITGGNIGLVGTPAIVAGYVQLTSVQSMEFNKKTPKEGIQFYIGIKIDPISGEFYESGN
                     YTDTLTITFVGL"
     gene            complement(4966..5457)
                     /locus_tag="PK105_07650"
     CDS             complement(4966..5457)
                     /locus_tag="PK105_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61626.1"
                     /translation="MKKPFLLVLIGILAASMAWGATINITANVSQILDVSVDASTWTI
                     PLDNSGTAITNASQGILTVKASKSNYTVTFSSTNSGALKNGTEAIPYKIQVNTSAWTE
                     GVQTNNLSSYTQLTTPQTIVFKKKTPTAGKTFGIDFNIEAYTEYYVDGLYTDTLGITV
                     SAP"
BASE COUNT         1520 a         1293 c         1104 g         1586 t
ORIGIN      
        1 atgctcccga aagcaaatag atagcaaaag acgcaatgcc tgcaaacagt cccgatccaa
       61 ggtaatagag caaaaattca taactaccaa ggctatgctc caccggccta ccgaagaaat
      121 atagccccag catatttaca attaagtgag tcaagtttgc atgagcaaac atgtaggaaa
      181 ataactgcca cacccagccc gaggcaacag cggctggatt tagagcaagc acgtatgtaa
      241 gcaaaggcca aatatattgc agagcaaaga tgatcacatt agcggcgatc aaatacaatg
      301 tcgcattgaa gtatttgaac ttgaaaggac ggcgcagcac agaatttcga ttcatgagtt
      361 aaaagtacag aaaaacgaga aatggaacaa ggacttggac tacagagttg ctgaacggcg
      421 ctataaacaa gcgccaaaag tcaaaacatg gacaaaaagc aaggatagct acttaaaaat
      481 gaaaggccgc ccggccttag cctggacggc cttcctgtca tcactctgtt acggattact
      541 ggccgctgac cgtcaaggta attatatccg tgtaggtccc agcctgatac atgccgcttg
      601 gggcatcaat gtcgatggca aacgcatatt ctgttccatc ttttggtgtc attgtctgtg
      661 cgcttgacct aatacccgcg gttgttggct gttgtttatt gaataaactg ccaacagtca
      721 ctttataagc gattttccca ctagtggctg tgtcaaccaa attgccatta ttctgactgc
      781 taaccgtaag tatccagtta ttgacattgg aaatcacctt catcttggat aaatcccaat
      841 gtgttgaact attatacgag atagagcccg tatagtttcc tggtacggtc agttgaaaaa
      901 cctggctgac agtgccttgg atcgttacat tgccggtatt tgtatccacc gcagcaaaca
      961 ccatccctaa cgacactacc gcaaacagag ctaccattgc aaactttttc attttgcgtc
     1021 tcccgcccgc agggtctatg ccccgcggat acattttgtg ttttttgtgg cttcttccaa
     1081 cggattcagc cactacactt tgatataagt ataatcgaca taaacatctt gtgcaatgag
     1141 tttttctcta tatttaagag cttagaactt ttggtctctc ttactaccat tgataatttt
     1201 tgatttaatc gtgaattagc aatttaatat gtacactgta aattgtagct atttaataga
     1261 atagagaaat tgccactttt gtctaatatc tctataaact taattattgt ttttcaatga
     1321 cgacactaaa gatgcattat gccagataaa gaaagctgtc attggaaagc aagccctcat
     1381 cctattctct tatcatcaaa aataaggcga atttgttggt cgagggccgt catcgtaaag
     1441 ggcttaacta gaagactgtc gaagccagct ttagcacatt tctccgcttc ttcctggtca
     1501 tgtcgcgggc tcatggcgat gagtacaggt ctgggcaagg cactctggag gcccttttgt
     1561 tgggatatat cttcagcccg aattttttgg gccagggcat aaccatccat ttcaggcatg
     1621 acacagtcta tgagcataat atcgaagctc tttaccctga gcgattctag agctgcttct
     1681 gcgcttgaga cagcccttgc ctcataacct ttcaatgtga gcattcgcaa gagaagatcg
     1741 cgatttactt cgctatcatc gacaacaagc accgatctgg cactcgcgat tttttcttta
     1801 ggcgcagcgc tttctttgtt ttcggcctca ttttcgattt cttgatcccg taatataatc
     1861 ttctcctgag caggtccttc aaccgtttct tttattggcc ttcctaacga agcgcttacc
     1921 aagttctctg ccaggggctc ttgcagaatc tcggcctttt taatcacttc tatagaagac
     1981 tcttgcaggg gttctgtttt tggcacgaaa ggctttatgg ttataagtgc ttctttggat
     2041 cctatgggag ctttgccctc tttctctaaa gatcctgcta gtatggcaag cccacagctt
     2101 aatagcacaa gtataagaag tcccgctccc catagcgcca cctgtctata tgggtacaat
     2161 gatgctacat aaatagtcca cgaaaaatct tctgatccga ttttcagccc agccgaataa
     2221 ccggaaagcg cactctcttc gccccaaaat agctccttgt ctcccttttc catatcggaa
     2281 gatgcaacgg caatggacag ccccgggaat tgcgaaacca aatccaaatc cgacaatagc
     2341 ttgtctgcat cgaatgcgat actcacaaaa ccccaaagtt cggagccctc tataacaggt
     2401 attcgcaaaa atgcgaggtt ctttccttct gcggatagct ctggcccctg caaaacggcg
     2461 ctcttctttc gcactgcttc gaccagagca cgcattctat caggattatc gagcaaatcg
     2521 tgaccaatcg aagctgaagc cctactctca ggaaagaaat atcttactat tgcccccgga
     2581 gctatcgtaa tcgagtctat ataaggcaac cgagcaagcg cctcatcggc cagtggtccc
     2641 agcgacgcat agttcctacc gctaccagca atgattaact cgcgtaagcc ttcaccgacg
     2701 gtgagcatct cttgcaggga ctcggagatt tgctgcgcta ttctcttgga gacgcttcca
     2761 agcagttctg attccgatcg gttcgcgctt ttgtatagca tgacaaaaaa gaatatcgca
     2821 gacacgcaaa acaatagcga aagcaagact attgacatct tggctatatt ctttttctgg
     2881 ctgctaataa agctcataat tcaattatat aaaaatatca taaaaaaggc cgcccattaa
     2941 gcgggcagcc ttctataact atgcaggata attctctcag gttgctgaaa gtgtaattgt
     3001 aatagtatcc gtataagaac ccgccaagat accgagcgct tccgctgtct ggtagaatac
     3061 ataaatgttg aaatatccct cagtgatcaa tggcgcattt ttctgtatag tatacaatcc
     3121 atccggttcg ctggtagaat taactatcgt ctctcctgta tcgccaaatc taaattcata
     3181 aggataaccg ctatcgcttc ctggctgtcc tgccgcttta accattttcc atgtattgtc
     3241 ggatttaatc tgaatttccc agttttgtac attggccttt attccgacgg caccgaggca
     3301 aacaccatta attgcacctg cattagtcgt atcaagagtc tcagtaaaac cagagcattt
     3361 agtgatactt agtatctggc tgacggttgc atccaaattg attgtgctag atcctgaagc
     3421 aaagactgga atagcaataa gcaataacac tagtcccgat agaaacactt tatttttcat
     3481 aaactttctc ctttaggcca ataatagcct aaaatttttt cataaattag ccaaatttga
     3541 cattttatca atgatccttt ttgacttata ccaagactcg attaaaatgt ccacatatat
     3601 agctcgatta aaatgtcccc ttttcactca ggctgagcga gtgaccagtt cctccttgtg
     3661 tggtagttgc tcgatttcga catactcgag ttctatagtt tggtagaaag atagacttcc
     3721 ctgagatagc gattggcatc ctcaatcgtc gagataccag ccagcctcag ttctttcacc
     3781 agccgttcct gatacactct atggcttcgt tcaacccgcc ctttcgcctg ttgtatgcca
     3841 taacgctcaa tccagcgccg gaggagtcgc atcgcatcgg acgtggttta ttcttcacag
     3901 aaaaatgaga aggtgatttc tgttggtcat ccaccatatt catcaggcag caggcacttc
     3961 ttcgtctctg ccacaaacgt aggcccaaag tctccatagt accatagtac tcctagcaaa
     4021 gacgcaccgc cgcctccatt atccccccat catagaccct tactgaggcc ttcccttggt
     4081 tacggtgcag caacccttcg tcgccttcct ccagatatcg ccggtagatc cgccaggggc
     4141 attatcatgc ggtttcaaca taggcagaaa ctaatacagg atgagttctc tccttcttca
     4201 taataaaacc cttctagaag taccctatat aaactacttc taggaagctc aacaagaaga
     4261 agttcatcga ctcaatcgcg aatagatgcg ttgttttaga cacatctaaa aagccgccat
     4321 caagcttctg cttgatagcg gcttttattc ctgcctcaat ccatgatctt caataatagt
     4381 tcacagtcca acaaaagtga tagtcaaagt atcagtgtaa tttccagatt cataaaattc
     4441 tcccgaaata ggatctatct ttattccgat atagaactga atgccttctt tgggtgtctt
     4501 tttgttgaat tccatactct gtacgctggt aagttgcaca tatccagcaa caatagcagg
     4561 tgttcccact aaaccaatgt tgccgccggt aatgcgtgtt gctttgatgt aatatggtac
     4621 ataatagtct gtgccttgtt taagacttcc gagattttgt gatgaaaaat cgacacgaaa
     4681 attttttcta ttgcttgagc gaatcgtcat tgttccttga ttatcatttt gataggtagt
     4741 gactccatca tttgcaaaca agatgtccca aatataagag tccatagtaa tagatgctcc
     4801 cggaggggga gcaactacta ctgttatccc tatgctcgcc tgtgcattta gcttgagtat
     4861 catgaatagg gcgaaaacca cgacaaccca ctttttcatt ttatgcacct tctaggtacg
     4921 atcaaagtta gagaaagata gaatcagcga aggaaagtac tttgatcaag gagctgaaac
     4981 tgtgatgcct aaggtgtctg tgtataatcc atcgacgtaa tattcagtgt acgcctcgat
     5041 gttgaagtcg atgccgaatg ttttcccagc ggtaggggtc tttttcttga agactatggt
     5101 ttgcggagta gttaactgcg tatacgagct caggttgttt gtctgtactc cttcggtcca
     5161 tgcgctggtg tttacctgga ttttgtaagg aattgcttcg gttccattct tgagagcacc
     5221 gctatttgtg gaagagaaag tcacggtgta gttcgatttg ctggccttaa cggtgagtat
     5281 gccttgagaa gcatttgtaa tcgctgttcc tgagttgtcc aagggaatag tccaggttga
     5341 cgcatcaacg ctcacatcaa gtatttgcga aacatttgcc gtgatgttga ttgtagcacc
     5401 ccatgccatg ctggcggcaa gaatcccgat aagtaccaag agaaaaggct ttttcataat
     5461 caacctccat agacgattat gataataaaa ttgcatttaa ttt
//
LOCUS       DAOOWY010000115         5488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_14979_length_5488_cov_14.523100, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000115 DAOOWY010000000
VERSION     DAOOWY010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14979_length_5488_cov_14.523100"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..646)
                     /gene="rpsD"
                     /locus_tag="PK105_07655"
     CDS             complement(11..646)
                     /gene="rpsD"
                     /locus_tag="PK105_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOU61627.1"
                     /translation="MARYIGPVCRLCRAENRKLFLKGDRCKSDKCPINKKRLPPGKAP
                     KTRMGKKSEYAVQLREKQALKRNYGLMERQFHNLFDESLRLPGKTGDNLIILLERRLD
                     NVVYRLHFASSRAQARQLVNHGHIAINGKRVDIASSLVKPGDVISVIGQAKKMDSIRH
                     ALEEVQKSGVMPWLEVNADEMTGRYVINPQRQDVTDTLDIKEQLVVELYSK"
     gene            complement(662..1054)
                     /gene="rpsK"
                     /locus_tag="PK105_07660"
     CDS             complement(662..1054)
                     /gene="rpsK"
                     /locus_tag="PK105_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOU61628.1"
                     /translation="MAATTTKTKKRKEKKSVYEGNVYIQATFNNTIVTVTDLNGNAIA
                     WSSSGSHEFRGAKKSTPFAAQTVTEAAIQKAMQAGLREAHVYVKGPGIGRESAIRQLG
                     VMGLKVKSINDVTPIPHNGCRPRKARRI"
     gene            complement(1070..1441)
                     /gene="rpsM"
                     /locus_tag="PK105_07665"
     CDS             complement(1070..1441)
                     /gene="rpsM"
                     /locus_tag="PK105_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOU61629.1"
                     /translation="MARIAGVDLPNKHVDIALTYIYGIGRASAQKICETTGIDANKKI
                     NELSNEEIAELRKVIENDYKVEGRLRTEVALNIKRLMDIGCYRGLRHRRGLPVRGQRT
                     RTNSRTRKGKRKTVANKKKAV"
     gene            complement(1458..1571)
                     /gene="rpmJ"
                     /locus_tag="PK105_07670"
     CDS             complement(1458..1571)
                     /gene="rpmJ"
                     /locus_tag="PK105_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOU61630.1"
                     /translation="MKVRTSVKKICDKCKIIRRNGVVRIVCSNPKHKQKQG"
     gene            complement(1624..2946)
                     /gene="secY"
                     /locus_tag="PK105_07675"
     CDS             complement(1624..2946)
                     /gene="secY"
                     /locus_tag="PK105_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOU61631.1"
                     /translation="MANTLVDIFRVKELKDRILFSLLWLAIFRLGTYLPIPGINASAL
                     QSYFAQQSSTGSGGITDYLNFFAGGAFKNFSLFMMGVMPYISAQIIMQLLIVIFPKLK
                     AIAEEEGGRRKISRWTKIGTIVVAIIQSFSVTVWADRIPNAIVMSNRTLYTIIAILTV
                     TTGTMFLVWIGEQITKRGIGNGISLLIFAGIVARLPNASYELIKSIQRGEINAVYAIV
                     VLAMFVAVVGLVVIEQQGERKIPVNYAKRIVGRRMYGAQNTYIPFKINPSGVIPVIFA
                     SSLLTFPLQIAQSFGVQAKWLRDFSYWLRPSGFWYNLFYVIFIVFFAYFYTQVTLNPQ
                     EISKNIRENGGSIPGIRSDKMEEYLTRVLNRIILPGALYLALIALIPTLVQQMFNFPS
                     ELAYLLGGTSLLILVGVDLDTMAQVEAILKMHHYDGLVRKGRIKSRNL"
     gene            complement(2964..3437)
                     /gene="rplO"
                     /locus_tag="PK105_07680"
     CDS             complement(2964..3437)
                     /gene="rplO"
                     /locus_tag="PK105_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOU61632.1"
                     /translation="MPDFYLHAPKGANKKKRIIGRGNSSGSGGTAGRGNKGQQSRSGG
                     KTYPGFEGGQMPLYRRLPGRGFSNKPFRKEYQIVNLCDLEAKFESGDSVDTLSLFMKG
                     LVKKPELPVKILGKGEIDIALMVSVDAVSSGAKAKIEAAGGKVETPIATGSSADA"
     gene            complement(3437..3619)
                     /gene="rpmD"
                     /locus_tag="PK105_07685"
     CDS             complement(3437..3619)
                     /gene="rpmD"
                     /locus_tag="PK105_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOU61633.1"
                     /translation="MPKIQITLVRSTIGQKPKARETVRCLGLRKIGSSTIQEASPQIL
                     GMVDKVKHLVEIKEVE"
     gene            complement(3622..4104)
                     /gene="rpsE"
                     /locus_tag="PK105_07690"
     CDS             complement(3622..4104)
                     /gene="rpsE"
                     /locus_tag="PK105_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOU61634.1"
                     /translation="MQEGYTEKLIQLNRTAKVVKGGRRFSFSALSVVGDKQGHVGFGF
                     GKANDVTEAIRKSVDRAKHAMVTVPVKNGTLPHEVTGIYKATKVYMKPACPGSGVIAG
                     GPVRAILECAGYIDIISKCIGSRTAVNVVRAVFDGLDKLLDAQEVAKNRGKSLKDMWS
                     "
     gene            complement(4144..4509)
                     /gene="rplR"
                     /locus_tag="PK105_07695"
     CDS             complement(4144..4509)
                     /gene="rplR"
                     /locus_tag="PK105_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOU61635.1"
                     /translation="MSVKELTDKRRKREKRKTHIRKSIFGAPDRPRMTVYRSNLHLYV
                     QVIDDMVGHTLSSVSTMEKQFRDLRPNVQSGNLIGEEIGKRLMEKGITQVVFDRNGYK
                     YHGVVKAIADGVRKAGVKF"
     gene            complement(4520..5062)
                     /gene="rplF"
                     /locus_tag="PK105_07700"
     CDS             complement(4520..5062)
                     /gene="rplF"
                     /locus_tag="PK105_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOU61636.1"
                     /translation="MSRIGMRPVAIPKGVKVTIEPTVFHVEGPKGKLSQEYCSDIKIE
                     IRDGFAVVERKDDSKQSKAYHGLYRSLLDNMITGVSQGFKRSLVISGVGYRAEVQGNF
                     LMLSIGFTNDMIVVIPEGLRVSVDQNGQRIIIEGIDYAKVGKFASEVRSLRKPEPYKG
                     KGIRYEEERIRRKVGKTGVK"
     gene            complement(5076..5474)
                     /gene="rpsH"
                     /locus_tag="PK105_07705"
     CDS             complement(5076..5474)
                     /gene="rpsH"
                     /locus_tag="PK105_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOU61637.1"
                     /translation="MSVSDPIADMLTKIRNANEARHEKVDIPTSKLKLEIVKILKTEG
                     YIKNFKKIQNEGQNFIRIFLKYDEKNDPIIHGLSKVSKPGRRVYSGYREMPRILNGYG
                     IAIVSTSDGVITGRKAVEKQVGGELICTVW"
BASE COUNT         1287 a         1460 c         1172 g         1569 t
ORIGIN      
        1 ccgctcctta ttatttcgag tacaactcga ctaccagctg ttccttgata tccagcgtat
       61 cagtgacatc ttggcgctgt ggattgatga catatcgacc agtcatctcg tccgcattga
      121 cttcgagcca tggcatgacg ccggattttt gaacttcctc tagggcatgg cggatcgaat
      181 ccatcttctt tgcttgtcct ataaccgaga taacatcacc cggctttacg aggcttgaag
      241 ctatatcgac tcgcttccca ttaatagcta tatggccatg attgaccaat tgacgagcct
      301 gcgcgcgtga acttgcaaaa tgtaagcggt aaacaacatt atccaaacgt ctctcaagga
      361 gaataataag gttatcgccc gtcttgccgg ggagcctcag agattcatcg aacaagttgt
      421 ggaactggcg ctccataagg ccgtaattgc gtttcaaggc ctgcttctcg cgtagctgca
      481 ccgcatattc tgacttcttt cccatgcggg tttttggggc ctttccagga ggaagccttt
      541 ttttgttgat aggacattta tcgctcttgc agcgatctcc cttcaaaaag agcttcctgt
      601 tctcggcgcg gcacagtctg catacaggtc caatatatcg agccattttt gtaaccccct
      661 cctagatgcg gcgagccttg cgaggcctac agccgttatg aggaatcggc gtaacatcat
      721 tgatcgactt taccttgagc cccataacac cgagctgtct aatcgcggat tcgcgtccaa
      781 ttccaggtcc tttgacatag acatgcgctt ctcgcagacc agcttgcata gccttctgta
      841 tggcggcttc ggtcacggtc tgtgcggcaa aaggtgtaga tttctttgca ccacggaatt
      901 cgtggcttcc ggagctcgac catgcaatgg cgttgccgtt gaggtctgtc acggttacaa
      961 ttgtattgtt gaaagtcgcc tgaatatata cgttcccttc atagacactc tttttttctt
     1021 ttctcttctt agtttttgtc gtagtcgcag ccacgttcat gctcctctgt taaacagctt
     1081 tcttcttgtt tgcaacggtc tttctcttgc ctttccgcgt acgcgagttc gtcctggtac
     1141 gctgaccgcg aaccggaaga cctctgcgat ggcgtaggcc acgatagcag ccaatatcca
     1201 tgaggcgctt gatattcaaa gctacttcgg ttcttagacg accctcgacc ttatagtcgt
     1261 tttctatgac tttgcgaagc tcagcaatct cttcattgga aagctcattg attttcttat
     1321 tggcatcgat gcctgtggtt tcgcaaatct tttgagcact tgcccgccca ataccataga
     1381 tatacgtaag agcaatatcg acatgtttat tagggagatc gactcccgca attcttgcca
     1441 tgagcttcct cctgctctta gccttgtttc tgtttatgtt taggattact gcagacaatc
     1501 ctaaccacac cattgcgccg tattatcttg catttatcac aaattttctt gacgcttgtt
     1561 ctaaccttca tgggttcccc ctattacaaa acacgcaatt aaatcctatt cgcgcaaaaa
     1621 tttctagagg ttcctcgact tgatacggcc tttgcgtaca agaccgtcat aatggtgcat
     1681 cttgaggata gcctcaacct gtgccatggt atcgagatcg actcccacaa gaatgagcaa
     1741 ggaagtacca ccaagcagat aagccagctc actcgggaag ttgaacatct gttgcaccag
     1801 cgtcggtata agcgcgatta gggctagata cagcgctccg ggaagtatga tgcgattcaa
     1861 gaccctggta aggtattctt ccattttatc gcttcgaatg cccggaatag atcctccgtt
     1921 ttcgcggata ttcttggaga tctcctgcgg gttcagtgta acctgtgtat agaaatatgc
     1981 aaagaagacg ataaaaatga catagaataa attataccaa aacccactag gtctaagcca
     2041 atacgagaaa tccctgagcc actttgcctg aacaccgaaa ctctgtgcaa tctgcaaggg
     2101 aaaagtaaga agactcgaag caaagatgac tggaattacg cccgaaggat tgatcttgaa
     2161 tgggatatag gtgttctgag ccccatacat tctgcgtccg acaattcgct tagcataatt
     2221 tacaggtatc tttctttctc cttgctgttc gatgactacg agccccacaa ccgcaacgaa
     2281 cattgccaac actactatcg cgtatactgc gttaatttct ccccgctgaa tgctttttat
     2341 gagttcgtag gaagcgtttg gaagccttgc cacaatacca gcaaaaatca aaagagagat
     2401 tccatttccg atgccgcgct tggttatctg ttcgcctatc caaacaagga acatagtgcc
     2461 tgtcgtaact gtgagtatgg ctattatcgt atagagcgta cggttggaca tgacgattgc
     2521 attgggtatg cgatctgccc atacggtcac cgagaaactc tgaatgattg caactacgat
     2581 cgttccaatc tttgtccagc gagaaatctt ccgacgacca ccttcttcct cggctattgc
     2641 tttcagcttg ggaaaaatga cgatcaatag ctgcatgatt atctgtgcgc tgatataagg
     2701 cattacgccc atcataaaga gcgaaaaatt cttgaacgcg ccaccagcaa agaaatttag
     2761 gtaatcggtg ataccgccgc taccagtgct ggattgctgc gcgaaatatg attgcagcgc
     2821 gcttgcattg attccgggaa tgggcaagta tgtacccagg cggaaaatcg cgagccatag
     2881 cagggaaaag agaattctat cctttaattc cttgaccctg aagatatcga cgagtgtatt
     2941 agccattcgc tcccgccttc atatcatgca tctgccgaag aacctgtggc tataggtgtc
     3001 tccactttgc caccagcggc ttctatcttt gcctttgcac ccgaagagac cgcatcgacg
     3061 ctcaccatga gcgcgatatc gatctcccct ttgcccaata tctttactgg caactcaggc
     3121 tttttcacga gccctttcat gaaaaggctc agggtatcca cgctatctcc gctctcgaat
     3181 ttggcttcta ggtcgcacag atttacaatc tgatattctt tgcggaatgg cttgtttgag
     3241 aagcctcgac caggcagacg cctgtaaaga ggcatctgtc caccttcaaa accagggtag
     3301 gtcttgccgc cagaacgcga ctgctggcct ttgttgccac gacctgccgt accaccagaa
     3361 cccgaactgt tgcctctacc aataatgcgc ttttttttgt tcgcaccctt tggggcgtgg
     3421 agatagaaat caggcatcac tccacctcct taatctcaac cagatgtttc actttatcca
     3481 ccatgcctag gatctgagga ctggcttcct gtatggtgct cgaacctatc ttccgcagcc
     3541 ccagacagcg gacagtttct cgcgctttag gcttttgacc aattgtgctg cgaaccaatg
     3601 ttatctgtat tttgggcatg attaactcca catgtctttg agcgactttc ctcggttttt
     3661 cgcgacttct tgcgcatcca agagtttatc caggccatcg aataccgcac gcacaacatt
     3721 tacggctgtt ctcgaaccta tacacttcga gatgatatcg atgtagcctg cacactccag
     3781 aatagcacgc acagggcctc cagcaataac accggagccg gggcaggctg gtttcatata
     3841 taccttggtc gccttgtaga tgccggtaac ctcgtggggc agcgtgccgt tcttgacggg
     3901 caccgtaacc atggcatgct ttgctctatc gacgcttttc ctgattgctt cggtgacatc
     3961 atttgccttg ccaaaaccga acccgacatg gccttgcttg tcgccgacca ccgacagtgc
     4021 tgagaaggaa aaccttcgtc ctcctttgac aaccttggca gtcctgttca gctgaataag
     4081 cttttcggtg tagccttcct gcataccgga atcatctttc cttgttcttt ccactgccgc
     4141 cccctagaat ttgacgccag cttttctgac gccgtcggca atcgctttca cgacaccgtg
     4201 gtatttatag ccgtttctat cgaagacaac ctgtgtaata cctttttcca tgagcctttt
     4261 accaatttct tcacctatca ggttaccgct ctggacatta ggtctgaggt cgcgaaattg
     4321 cttttccatc gtagagacgc tcgaaagggt atgaccaacc atatcgtcga taacctgcac
     4381 atagagatgc agattcgaac gatacaccgt cattcgaggc ctatcgggag ccccaaaaat
     4441 gctctttcga atatgggtct tgcgtttttc acgcttgcgt ctcttatctg tcagttcttt
     4501 tacgctcatc ctatctgcct cacttcacgc ctgtcttgcc gacttttctg cggattcgct
     4561 cttcttcata gcgaattcct ttgcctttat atggttcggg cttccgaagc gatcgcactt
     4621 cgcttgcaaa cttgccaacc ttggcatagt ctataccttc gattattatt ctctggccat
     4681 tctgatcgac tgagactctt aagccttcgg gaatcaccac aatcatatca ttcgtaaatc
     4741 cgatactgag cataaggaag ttgccttgca cttctgctcg atatcctaca ccagagatca
     4801 cgagagatcg cttaaagcct tggcttaccc cggttatcat gttgtccagc aagctgcgat
     4861 atagaccatg ataagccttg gactgcttgg aatcatcctt gcgctccact acggcaaatc
     4921 catctcttat ttcgatcttg atatcggagc agtattcctg agagagcttc cctttcggtc
     4981 cttcgacgtg aaaaaccgtc ggctctatag tcacctttac acctttggga attgctacag
     5041 gtcgcattcc aattctcgac atactttacc tcgccttacc agaccgtaca gatgagctcg
     5101 ccgcctacct gcttttccac agcctttcta ccggttatga caccatcgga agtcgagacg
     5161 atggcaatac catagccatt gagaatccgc ggcatttcac gatacccgga atagacgcgt
     5221 cgccctggct tagagacctt gctcaatcca tggattatgg ggtcattctt ctcatcgtat
     5281 ttgagaaaga tcctgataaa attctgcccc tcattctgga tcttcttgaa atttttgata
     5341 tagccctcgg ttttgagaat ctttacgatc tcaagcttga gcttggaagt cgggatatcg
     5401 actttctcat gccgagcttc atttgcgttt cgaatcttgg tcagcatgtc cgcaatggga
     5461 tcagatacac tcattgcacc ctcctacc
//
LOCUS       DAOOWY010000116         5488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_14980_length_5488_cov_8.548132, whole genome shotgun sequence.
ACCESSION   DAOOWY010000116 DAOOWY010000000
VERSION     DAOOWY010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14980_length_5488_cov_8.548132"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..2079)
                     /locus_tag="PK105_07710"
     CDS             complement(4..2079)
                     /locus_tag="PK105_07710"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOU61638.1"
                     /translation="MAYINKLLTENFLYYASYVIMDRAIPELDDGLKPVQRRILHSLF
                     EMDDGKFHKVANVVGHCMKYHPHGDASIASALINLANRGLFLDTQGNFGNPITGDEAS
                     APRYIECKCSPFAKEIFYHPKITRYIDSYDGRNKEPLVFPAKIPVILILGAEGIAVGM
                     STRILPHNPIEVIKAEIAYLQGKAFSLSPDFPAGGTADCSEYADGNGKVRVRATIDSS
                     DPKKIVIKEIPYGSTTESLINSIEEAVRAGHLKISSISDFTTSKVEIELKLARGVYAR
                     DVVDALYAFTECERSISCNCLVIKEGKPVVTTVSETIKYHAARLLDLLRQELELERDE
                     LKNELFSRTLERIFIEEKIYTSIETKRTNDTVIKAVLDGLKPFTKELSRKIEVEDVER
                     LLKIPIRRISLYDIEKAKREMEQIERRISENEMHLRHIVDYAIDILQSYIEMIEKTCP
                     RKTKLRAFKRIEARAVARHDFALKYDAEGGYLGTEVSGEKLLEASKFDKILILRNNGI
                     YTIIPLPDKFFAGQKLLWTAIAKKESLSKTTITLIYSLSGENGAYIKRTIIDSWLTGR
                     DYSLLPETAVILGFSTASEFDFKLEYKPKSRSKKTYEVFHAKNYTVRNRSAQGIKLTD
                     REIIRFEILTTQESDNKRKQEDLDASTAHISKKKPNREARSKKEIPPLETISEGLLKA
                     ALKKQKKEN"
     gene            complement(2133..3938)
                     /locus_tag="PK105_07715"
     CDS             complement(2133..3938)
                     /locus_tag="PK105_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOU61639.1"
                     /translation="MTGNKSTYDESKIKTLSSLEHIRLRTGMYIGRLGDGLSPDDGIY
                     ILLKEIIDNSVDEFIMGAGNQIVINLQGRRMRVRDYGRGIPLGKVIECVSVINTGAKY
                     NDDVFQFSVGLNGVGTKAVNALSSEFRVVSYREGHAFEAKFVRGLRVDSREFETREKT
                     GTLVEFVPDEQIFGEYTFVFEFIEKRLWSYACLNPGLKLSINGKEFQTEKGLQELLST
                     ELGGRALYDIGWYRGDHIEFAFTHTREYGEEHFSFVNGQYTSAGGTHLSAFREGFLKA
                     INEFFQASYHGEDVREGLAAAISIRIKNPIFESQTKNKLGNSDVRPWILQEVKKGVDE
                     ILRRNPQAAKILQEKILTNEKLRTELNIVRKEAKEAAKRIELKIPNLKDCKIHLGDGD
                     LEDQSTIFITEGLSAAGSMVSARNVYTQAIFSLRGKPENMFERPRSAIYKNGELYNLM
                     MALGIENNVENLRFARIVIATDADYDGFHIRNLLLTFFLTFFEELVVQGRVYLLETPL
                     FRVRTKKETIYCYSIHERDDGIKKLGTGSEVTRFKGLGEISPAEFGQFIGKNMRLVKV
                     DIQTLSSVPELLDFFMGKNTPQRRQFIMDNLVLDL"
     gene            4051..5313
                     /gene="larA"
                     /locus_tag="PK105_07720"
     CDS             4051..5313
                     /gene="larA"
                     /locus_tag="PK105_07720"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOU61640.1"
                     /translation="MHFELKYGDGSLPLDIEQSRILSVVLPNKRRSLADARNAVASAL
                     AQPEGTAPLLEMLIDRKPKKLVIIVNDITRPTPYSILMPPLVEVIEKAGIPDENVTIL
                     IATGIHDLHTHEQNLIVYGEEMCRRFRIISHDANDSSMLSHKGKLPSGYDYWLNRIVD
                     EADFLITVGVVMPHYFAGFSGGRKSILPGVVGKETVERNHARMVEIMDNLPPIRENPI
                     SLEMIYAARIAGVDFILNVVVDECESIVEVVAGDLEKAWYKAVDVSQAMYMIPIPEQA
                     DITICSASGFPRDINLYQAQKALDHADRATKRGGTIILVAECRNDYGEKVFERFMTAG
                     YSPEEIMSHIKAHFVIGGHKAYGFAKVAAEKRVIMVTSLSESIVASCFAQKASSLAGA
                     IDMAIAQQSPGAKFIIMPEGSITSPVII"
BASE COUNT         1361 a         1201 c         1220 g         1706 t
ORIGIN      
        1 ttcttaattt tctttcttct gcttttttaa agcagctttg agcaagcctt ctgaaattgt
       61 ctccaaagga ggaatttcct ttttcgatct tgcttcccta tttggctttt tcttggagat
      121 atgtgcggtg gaagcatcga ggtcttcttg cttgcgtttg ttatctgact cctgagtggt
      181 gagtatttca aaacggatta tttctctatc cgtgagcttt attccctgtg cactcctatt
      241 tctcaccgta tagtttttgg catgaaacac ttcataggtt tttttgcttc ttgatttagg
      301 tttgtattcc agtttgaagt caaattcgct agcagttgaa aatcctagaa tcaccgctgt
      361 ctccggtaac agactatagt ctctgccggt aagccagctg tctattatag ttctctttat
      421 atatgccccg ttttcgccgg ataatgaata gatcagcgtt attgtcgttt tagagaggct
      481 ctcctttttc gctatcgcag tccataacaa cttttgtcct gcaaagaact tatccggaag
      541 agggataatt gtatatattc cgttgtttct caaaatgagg attttgtcga atttcgaggc
      601 ttccaagagt ttttcgcccg aaacctctgt tcccagataa cctccttcag cgtcatattt
      661 tagcgcaaaa tcatgccgcg ctacggctct ggcttctatt ctcttgaatg ctctcaattt
      721 ggttttgcgt gggcaggttt tttctatcat ctcgatatag ctctgcagta tatcaattgc
      781 ataatctaca atatggcgca aatgcatttc attctcggat atccttcgtt cgatctgctc
      841 catttctctc ttggcttttt ctatatcata gagagatatc ctgcgaatgg gtatcttgag
      901 gagtcgctcc acatcttcca cttcgatttt tcgagaaagc tcttttgtaa atggctttag
      961 tccgtctagt accgccttga taacggtatc attggtcctt ttggtttcga tcgatgtata
     1021 gattttctct tcgataaaaa tgcgttcgag cgttctagaa aagagttcat tctttaactc
     1081 atctctttct agttcgagct cctggcgcag caggtcaagt agtcttgccg catgatattt
     1141 tatggtctcg gatacagtgg taacgacagg ctttccttct ttgataacca ggcagttgca
     1201 ggagatcgat cgctcgcatt ctgtaaaggc atagagcgcg tctaccacat cccgtgcgta
     1261 tactcctctt gccagcttta gctctatttc gactttgctg gtcgtgaaat cggagatgga
     1321 agagattttg agatgaccag ccctgacagc ttcttcgatg ctgtttatga ggctttcggt
     1381 agtggaaccg taaggaatct cttttataac gatttttttt gggtctgagc tatcaattgt
     1441 agcgcgaact ctaactttcc catttccatc tgcatattcg ctgcaatctg cagttcctcc
     1501 tgccggaaag tccggagaaa ggctaaaggc ctttccctgg agatatgcaa tctcggcctt
     1561 gattacttcg atgggattat gcggcagtat ccttgtgctc attcctaccg caataccttc
     1621 tgctcccaag atcaaaataa caggaatttt tgcaggaaat acgagtggtt ctttatttct
     1681 tccatcatag ctatctatat agcgagtaat cttaggatga tagaaaatct ctttggcaaa
     1741 tggagaacat ttgcattcta tataacgggg ggcggaggct tcgtctccag ttatggggtt
     1801 tccgaaattc ccctgtgtat ctagaaataa gcctctgttc gcaaggttta tgagagcaga
     1861 agcgatagaa gcgtctccat gggggtggta cttcatgcaa tgcccaacaa catttgcgac
     1921 tttatggaat ttgccatcgt ccatttcaaa gagggagtga agaattcgac gctgtactgg
     1981 cttaaggcca tcgtccagct ccgggattgc cctgtccata attacataag aggcatagta
     2041 gaggaagttt tcggttagca acttatttat ataggccact ctattgtcta tcctttcata
     2101 gcttttccgg tagattcctg gttttcaaaa cctcaaagat ctaataccag gttgtccatt
     2161 atgaattgtc gcctctgtgg tgtatttttt cccataaaga aatctaggag ctccggaaca
     2221 gatgataagg tctgaatatc tacctttact aatcgcatgt ttttcccaat gaattgtccg
     2281 aattccgcgg gacttatctc gcctaagcct ttgaaccgtg ttacttctga tcctgtaccg
     2341 agtttcttta ttccgtcgtc tcgttcatga attgaatagc agtatatagt ctcttttttt
     2401 gtccttactc taaaaagggg tgtctcgagc agatagactc gtccctgaac gaccaattct
     2461 tcgaagaagg tcaaaaagaa tgtcagaagg agatttcgta tgtgaaaacc gtcataatca
     2521 gcgtccgtgg cgataacgat gcgagcaaag cgcaaattct cgacattatt ctcgatgcca
     2581 agggccatca tgagattgta gagctctcca ttcttataga tcgcagatct agggcgctcg
     2641 aacatattct ctggtttacc gcgtaaacta aaaattgctt gagtgtatac gttccgggcg
     2701 ctcaccatgc ttccggctgc ggagaggcct tcggtaatga atatcgtcga ttgatcttcc
     2761 aggtctccgt cgccaaggtg tatcttgcag tccttgaggt ttggtatttt gagttcaatt
     2821 ctctttgcgg cttcttttgc ctcttttcga acgatgttca gttcggtgcg cagtttttcg
     2881 ttggtaagaa tcttttcctg gagaattttc gccgcctggg ggttgcgacg cagtatttca
     2941 tctacgcctt ttttgacttc ctgtagtatc catgggcgga catcgctatt gccgagcttg
     3001 tttttggtct gactttcgaa aatcggattc ttgattctaa tggagatagc cgccgcaagg
     3061 ccttcgcgga catcttctcc atgataagat gcctgaaaga actcgtttat tgcctttaga
     3121 aatccttcgc ggaaggccga gaggtgggtt cctccagccg aggtatattg accatttaca
     3181 aaagagaaat gctcttctcc atattccctg gtatgggtga aagcaaattc aatatgatct
     3241 cctctatacc agccaatgtc gtagagagct ctaccaccga gctctgtcga caatagctcc
     3301 tgaagccctt tttcggtttg aaattcttta ccattgatag atagtttaag gccagggtta
     3361 agacaggcat agctccaaag acgcttttcg atgaattcaa atacaaaggt gtattctccg
     3421 aagatttgtt catctgggac gaattctacg agtgttcctg tcttctcgcg agtctcgaat
     3481 tctcgggaat caacgcgcaa gccccgaacg aatttggctt caaaagcatg tccttctcga
     3541 taagaaacaa ccctgaattc gcttgataat gcatttactg cctttgtacc tacaccgttc
     3601 aatccgacag agaattgaaa tacatcgtca ttgtattttg cgccagtgtt tatcaccgat
     3661 acacattcga tgaccttccc taaaggtatg ccgcgtccat aatctcttac gcgcattctt
     3721 ctgccctgga ggttgattac gatttggttg ccggcgccca tgatgaactc gtctactgag
     3781 ttgtcaatta tctccttgag tagaatgtat attccgtcgt cggggctcag gccatcaccc
     3841 agcctcccaa tatacatgcc agttcgcagc cttatgtgtt ccagcgagga caatgttttg
     3901 atttttgatt catcgtatgt cgatttattg ccagtcacgc taggtattct atcatgatgg
     3961 acaatagagg gtatagagta ttttgtcttc gacgattata tgatagacct ttgattatat
     4021 agaaagaaat tcaaatataa ggcggtaatc atgcatttcg agctaaagta tggagatgga
     4081 agtcttccac tggacatcga acaatcaagg attctcagtg ttgtgcttcc caacaaaaga
     4141 agatcacttg ctgatgctcg caatgctgtt gcaagtgcgc ttgcacaacc tgaaggcaca
     4201 gcccctcttt tggagatgtt aatagaccgc aagcccaaga agcttgtaat cattgtcaac
     4261 gacattacga gacccacccc ttattcgatc ttgatgcctc ctcttgtcga agtgatagaa
     4321 aaagctggga ttcccgatga aaatgtgaca attctgattg ctacagggat tcacgatctt
     4381 catactcatg aacagaatct tattgtatat ggcgaagaga tgtgtcgtag attcaggatt
     4441 atttcgcatg atgcaaacga ttcctcaatg ctatcgcaca aaggcaagct gccttcggga
     4501 tacgactatt ggctgaatag gattgtggat gaagcggatt ttcttatcac ggtcggtgtt
     4561 gtcatgccgc attattttgc aggattctca ggcggcagaa agtccatctt gccaggtgtt
     4621 gtcggaaagg agactgtaga aagaaaccat gcgcgtatgg tcgaaatcat ggataatctt
     4681 cctccgatca gggagaatcc gataagcttg gaaatgatct atgcagcccg aattgcgggc
     4741 gtggatttca ttttgaatgt tgtggtggat gagtgcgaga gcatagtcga agtagttgcc
     4801 ggcgatcttg agaaggcctg gtataaggca gtagatgtga gccaagcgat gtatatgata
     4861 cctattcctg agcaggcgga tatcacgatt tgtagtgctt caggatttcc cagggatatc
     4921 aatctatatc aagctcagaa agccctggat catgctgacc gtgccacaaa gaggggaggg
     4981 actatcatac ttgttgcgga gtgtcgcaat gactatggag agaaagtgtt cgagagattc
     5041 atgactgcag gctattcacc agaagagatc atgtcgcaca tcaaagcgca ttttgtcata
     5101 ggtggacata aagcctatgg ttttgcaaaa gtggccgctg agaagagagt aataatggtc
     5161 acctcccttt cagagtcaat cgtggcaagc tgctttgctc aaaaagcttc ttcgcttgct
     5221 ggagcgatag atatggccat tgcccagcaa tcaccggggg ctaagttcat aatcatgccg
     5281 gaaggaagta ttacctcgcc agtcatcata taaaataagg cttatcccca agaaaaataa
     5341 gcaaaaccaa ccgcgcgcca ttagagcggg tgcgcaatgc tcaatgtgca atgtgcaatg
     5401 ctcaatgcgc aatgcgcgat gtgctttcta catgggcttt tatgtaagct cattctctat
     5461 aaaggctcca agcttgaggt aatctcta
//
LOCUS       DAOOWY010000117         5314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_15591_length_5314_cov_9.718388, whole genome shotgun sequence.
ACCESSION   DAOOWY010000117 DAOOWY010000000
VERSION     DAOOWY010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15591_length_5314_cov_9.718388"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PK105_07725"
     CDS             <1..775
                     /locus_tag="PK105_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61641.1"
                     /translation="KLDAVQKVYYLDTVEVEAIRDISLEIHSGDFVSIAGPSGSGKTT
                     ILNLIGCVDKPTSGTVIVNGQRTSDLDDDRLTELRHRSIGFIFQTFNLIPVLNIRENV
                     ELPLLLDGLSENETKAEREKWVDFLIDSVGLKDRMLHKPAELSGGQRQRVAIARALVM
                     KPAIVLADEPTANLDSATGESILRLMRKMNETFGTTFIFSTHDPDIVEEADHIIRLKD
                     GIIVEDFRINEDRSGDTLNFRNSIRATGTKATGTEGSQA"
     gene            772..2274
                     /locus_tag="PK105_07730"
     CDS             772..2274
                     /locus_tag="PK105_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61642.1"
                     /translation="MISARLAWRNICLHRKKSLIIGLIMGFGILILFVGNSLIDTTIS
                     GLRRMFVEGYTGDLMVTGPTEFSTTVLGETAGSEEVLPHIAQIKKYEDFLEARQDVAS
                     ILPILSGQVSFGIGEEELGSGYCFGVNIFDYRAFFPDNLTLVSGEWPEESDEPWVLLS
                     EPTYALLQQSSDIEVKPGTKIIMTALANSAGTVIREVSVKGIIKFNQSNAQLSGITLV
                     DADTMRDLLGFASLRDGAPQLSEHEAAFLTDFDPDSLFDNLFASQTITTEGEGQSELG
                     LEPSKLDVSEDPGQETSQASSTTTVIAEIVPAWQFLLIRAKNGSNTSKLLDDLEAFSE
                     ELGDGDRVQDWIAGAGKVARTAVTIRLVFDLLVAVIAVVVVMIMMNVLVISVNERLHE
                     IGTLRAIGAKRHVVRSMILYETTFMVLIAGAVGLILGYILLLIMGKVGIRAPNLFFEA
                     LFGGEILRPLISIGAAGRAFFWVLAMALVASWYPTRVAVRIEPVVAMRGD"
     gene            2277..3584
                     /locus_tag="PK105_07735"
     CDS             2277..3584
                     /locus_tag="PK105_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61643.1"
                     /translation="MHFSNFKLHLKLAARNLRRQPNRTIALGGAVAFSALVMTLISGF
                     VGGMDKAIQDNVTLYSGGHILISGYTPSWSGRLQNRFIDDEIAKTVESVIPDVRTVSP
                     LAQARATVVFGTREIQLTLRGVDWTKDELYHDSLILSSGDWEALNEQDRSMLMSSQTA
                     DRFGLAIGDQVVVRLTTASGQQNVTDYAVSAIYDDAASGGMTTAFVSFGDLIADLNMK
                     ANEQQQIAIFLKDSTDAERAAKAIAEALTEKGYSIASGTSGSEQASQRRMNSEKAVYS
                     ISTVQELAGEIGSALGSIRWIGYAVFILMLLVSATGIANSYQMVLLERTKEIGMLRCI
                     GFKKKDVFASFIFEGILLAGAAAFAGIICALPIGLGISFIPFDPHGDFGAALVKGHLR
                     FAPTLDQLLIILVFIMIVAIVAVFLPARKAAEVVPVEALRITA"
     gene            3626..4543
                     /locus_tag="PK105_07740"
     CDS             3626..4543
                     /locus_tag="PK105_07740"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOU61644.1"
                     /translation="MLRFYEKRTWFFSSKSIRNISLVLLGFVIVMCAQAQGGPPPGGP
                     KGGGGPMAGIGLPLGFLEADKDYKFPDDITKYTANDFLMLVRRTDIRSSFYNSDMNAT
                     ITTVNVSPTRGTFVTKEQIFRRDKDDAFLMLTLEPESRKGQGMLRVDNNMWRYDPTSR
                     KFTHTTLKDTYENTTVTNNDFRRWQRSIDYSVEKLSVGTLGNYKVIIGELKANNDEVP
                     FPYIKMYIEADRKIVLKVEEYSLSMKLLRTAYYTQYVQIGDSFVPTVQIFQDGLVPEK
                     RSQVTYTNISTKSLPDYYFTKEYLERVSQ"
     gene            4555..>5314
                     /locus_tag="PK105_07745"
     CDS             4555..>5314
                     /locus_tag="PK105_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61645.1"
                     /translation="MKTRILKGLIFLIVMTLFLISTSGIWAQASGTGTSLIPPEVIDE
                     NELFGGPKDIVTVIDPSVASAGTVQLVQDTKAYPVFLIGGSAGAGLYGSYTPLGSSSS
                     DTQTLYGAVDISDLEFDYLPAENLHFSISSNLLAVPNQILAASASAYADLRQSDLVRL
                     YVSGSFNYDPSSYLSTNEENTSPTFSLDELFVDTHIEETVFFRLGKQRISWGVGNWFK
                     PSDILSLSAIDPDDPTAAREGPFAFKVDVPMKLNH"
BASE COUNT         1393 a         1171 c         1317 g         1433 t
ORIGIN      
        1 aaaattggat gcggttcaaa aggtctatta tttggatact gtcgaggtcg aagctatcag
       61 agacatttcg cttgaaatac actctggcga ttttgtaagc attgcgggac cttcaggttc
      121 cggtaagacc accattctca atttgatagg ctgtgtagac aagccaactt caggcactgt
      181 gatagtaaat ggacagcgta caagcgatct cgacgacgat agactcacag agttgcgtca
      241 ccgctctatt ggattcatct ttcagacctt caatcttatt cctgtcctca atatccgcga
      301 gaatgtggaa cttcctcttc ttctcgatgg cctttcagaa aatgagacca aagcagaaag
      361 ggaaaagtgg gtggattttc ttatcgatag cgttggtctt aaggatcgta tgctgcataa
      421 gccagcagag ctctcgggag gccagcgtca acgtgtcgcg attgctcgcg ctctcgttat
      481 gaagcctgca attgtgcttg ctgatgagcc tactgctaat ctggactctg ctacaggcga
      541 aagcatcctg cgcctaatgc gcaagatgaa tgaaaccttt ggcacgacct tcatcttcag
      601 tactcatgat ccggatatag tcgaagaagc cgatcatata atacgtctca aagatggcat
      661 aatcgtggag gacttcagga taaatgaaga tcggtctggt gatacattga atttccgcaa
      721 ttctataaga gctactggca caaaagccac tggcacagaa ggcagtcaag catgatttct
      781 gcacgtcttg catggaggaa tatttgcctt caccgaaaaa aatccctcat tataggtcta
      841 ataatgggat ttggaattct aatactattt gttgggaatt ctctgatcga tacgacgata
      901 tccggcctgc gccgcatgtt tgtggaaggt tacaccggcg acctgatggt aactggtcca
      961 acagaattta gcacaaccgt gcttggcgaa accgcaggca gcgaagaagt gctgcctcat
     1021 attgctcaaa taaagaaata cgaagatttt ttggaagcac gccaggatgt tgcctcgatt
     1081 ctcccgatcc taagtgggca agtatccttt ggaattggtg aggaagaact cggatcgggt
     1141 tactgttttg gagtgaatat ctttgattat cgggcttttt ttcctgataa cctgactctt
     1201 gtgtcgggag agtggcctga agaaagcgat gaaccatggg ttcttctatc cgagcccaca
     1261 tatgcattac ttcaacagag cagcgatatc gaagtcaagc ccgggacaaa gatcatcatg
     1321 actgcattgg cgaattcagc gggcacagta atacgcgaag tcagtgtgaa gggaattatt
     1381 aaattcaatc agagcaatgc gcagctttcc ggcatcaccc ttgtcgatgc agacacaatg
     1441 cgtgatctct tgggttttgc aagtctgcgc gatggagctc ctcagttgag tgagcatgaa
     1501 gcagccttct tgactgactt cgatcccgat agcctattcg ataatctgtt tgcgagccag
     1561 acaattacga cagaaggcga agggcaatcc gagctaggtt tggagccttc aaaattggat
     1621 gtttctgaag atcctggcca agagacatct caggcttcaa gcacaaccac cgtcatcgca
     1681 gagattgtac ctgcctggca gtttcttctt atccgcgcaa aaaatggttc taatacttcc
     1741 aagcttttag acgaccttga agctttttcc gaagagcttg gagatggaga tcgtgtgcag
     1801 gattggattg caggagccgg aaaggtcgcc aggacggcgg taacgattcg gcttgtattc
     1861 gatctattgg ttgcggtcat tgcagtcgtc gtcgtcatga taatgatgaa tgtgcttgtc
     1921 atatccgtca atgagcgcct ccacgagatt ggcacactca gagccatagg agccaaacgg
     1981 catgttgttc gcagcatgat tctatatgag accacattca tggtgcttat cgctggggca
     2041 gttggtctaa ttctagggta tatcttgctc cttattatgg gaaaagtcgg cattagagcg
     2101 ccaaatctct ttttcgaagc tctttttggg ggagaaatcc taagaccgct tatatctatc
     2161 ggagcggctg gtcgtgcgtt cttttgggtt ctagccatgg cattggttgc ttcctggtat
     2221 ccaacaaggg tggcagtgcg aatcgaaccg gttgtcgcaa tgaggggaga ctgaatatgc
     2281 atttttcaaa ttttaaactc catttgaaac ttgcggctcg gaaccttcgt aggcagccca
     2341 atagaacgat tgcgctcgga ggagcggtgg cttttagtgc actcgttatg actctgattt
     2401 cagggtttgt gggcggaatg gataaggcaa ttcaagacaa tgttaccctt tattccggcg
     2461 ggcacatatt gatttcgggc tatacaccta gctggagcgg taggcttcaa aatcgattta
     2521 tagatgatga gatagccaaa actgtagaat ccgttatccc tgatgtcagg acggtttctc
     2581 ctcttgctca ggcaagggct acggtagtct ttggaactag agaaatacag cttacgctcc
     2641 gtggtgtcga ctggacaaaa gatgagctat atcacgattc cctcattcta tcgagcggag
     2701 attgggaagc gctgaatgag caggatagaa gcatgctcat gagctctcag acagcagatc
     2761 gattcggcct tgcaatcggg gatcaggtgg tcgtgaggct cacaacggca tccggtcagc
     2821 aaaatgttac agattatgcc gtttcggcga tctatgacga tgccgcatcg gggggcatga
     2881 caactgcttt tgtttctttt ggggatctaa tagcagatct gaatatgaaa gcaaatgagc
     2941 agcagcagat agcgattttt ctaaaggaca gcaccgatgc agaacgcgcg gccaaagcca
     3001 ttgccgaagc gcttaccgaa aaaggatata gcattgcctc tggaacctcc ggaagtgagc
     3061 aggcaagtca acgccggatg aattccgaaa aggctgtcta ttcgatttcc acagtacagg
     3121 agctcgctgg ggaaataggc tccgctctcg gctccattcg ttggattgga tacgcagtgt
     3181 tcattcttat gctgcttgta agcgctactg gcattgccaa ttcatatcag atggtgctcc
     3241 tcgagcggac taaggaaata ggtatgctac gatgcattgg tttcaagaag aaagatgtat
     3301 ttgcatcatt tattttcgaa ggaatcttgc ttgctggagc agcagcgttt gcaggaatta
     3361 tatgcgccct gccaataggc ctgggtatta gttttattcc cttcgatcca catggagact
     3421 ttggagcagc cctggtaaaa ggacatttgc gatttgctcc aaccttggat cagcttctaa
     3481 taatcttggt gtttatcatg attgtggcta ttgtcgcagt ctttttgcct gcccgaaagg
     3541 cggctgaagt agtgcccgtc gaggcactta ggatcactgc gtaatgctta tgatattgcg
     3601 attgcgcaga aactaggagc gtttgatgtt acgattctac gagaaaagga cttggttttt
     3661 ctcatcaaag agcatcagaa atataagcct tgtactcctt gggtttgtta ttgtcatgtg
     3721 tgcgcaagct caaggtggcc cgcctccggg cggaccaaaa ggtggcggag gacctatggc
     3781 tggcattggg ctcccgctgg gctttctgga agcagataag gactacaaat ttcctgatga
     3841 tataacaaaa tatactgcaa atgactttct aatgctcgtt cgccggacag atatccgttc
     3901 tagcttttat aacagcgata tgaacgctac tatcactacg gtaaatgtga gcccgacgcg
     3961 cggtaccttt gtaaccaagg aacaaatctt caggcgggac aaagatgatg cattcttgat
     4021 gctcaccctg gagccggaat cccgcaaggg acaaggaatg ctgcgcgttg ataataatat
     4081 gtggcgctac gatccaacaa gccgaaaatt cactcacaca accctcaaag acacttatga
     4141 gaatacgaca gttactaata atgattttcg tcgctggcag cgctcgatag attattctgt
     4201 ggaaaagctc tctgtgggca ctcttggaaa ctacaaggta ataatcggag aactcaaggc
     4261 caacaatgat gaagttccat ttccttatat caagatgtat atagaagcgg accgaaagat
     4321 agtgttgaaa gtagaagaat atagcctatc catgaagctc ttgcgcacag cttattatac
     4381 ccaatatgta cagataggag attcctttgt gcctacggtg cagattttcc aggatggcct
     4441 tgttcccgaa aagcgctctc aggttaccta taccaatatc tccacaaaat cgctccctga
     4501 ttattacttt accaaagaat atctcgaacg ggtgagtcag tgaggagcag aggcatgaaa
     4561 acaagaatac taaagggctt gatatttctg atagttatga ctttatttct tattagcaca
     4621 tccggtatct gggctcaagc ctctggcaca ggaactagct tgattcctcc tgaagtcata
     4681 gatgaaaatg agctatttgg cggccctaag gatattgtga cggtaataga tccgagcgta
     4741 gcttcggcgg gaaccgtgca acttgtccaa gataccaagg cttacccggt tttcttgatc
     4801 ggcggatcgg ctggagctgg tttatatggt agttatactc cgcttgggag cagctcctca
     4861 gatacacaga cgctctatgg ggcggtagat atttctgatc ttgaattcga ctatcttccg
     4921 gctgagaacc tgcatttttc catatccagc aatcttcttg cagtaccaaa ccaaattctt
     4981 gctgcgtctg ccagtgcata cgctgatttg cgtcagagcg atctcgtgcg actctatgtc
     5041 tcaggctcat tcaactacga tccatcgtcc tatttatcta ccaacgaaga aaatacctct
     5101 cctaccttta gcctggatga actttttgta gatacacata tagaagagac ggttttcttt
     5161 cgtctaggta agcagcgcat tagttgggga gtaggaaact ggttcaaacc ctctgatatt
     5221 ctgagccttt cggctattga tccagacgac ccaacagcag cgcgcgaggg gccctttgca
     5281 ttcaaggtgg atgtgccgat gaagctaaat cacc
//
LOCUS       DAOOWY010000118         5184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_16139_length_5184_cov_6.597192, whole genome shotgun sequence.
ACCESSION   DAOOWY010000118 DAOOWY010000000
VERSION     DAOOWY010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16139_length_5184_cov_6.597192"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..2475)
                     /locus_tag="PK105_07750"
     CDS             complement(31..2475)
                     /locus_tag="PK105_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOU61646.1"
                     /translation="MVERQKTQKTKDQATTQYAQGGRRLSQSAKELRDKIKRIISQAQ
                     SEHREALTELEGMSILAAMGVQTPRRWIVQSANEFLEKIGAEGAIDAPLQSPFPGSKA
                     VIKVISSKVLHKTEVQGIEIVDNTATAIADSLDRMEARFKNVPRDGFTVNEFIAFEPK
                     LGHEMILGYRFTPDFGPIVTFGPGGIYTEYLASKFKPGMANLFLSPRINDMAVLESLL
                     RNNVIYGLLCAGLRNTRPELEEKSLIEAIQRFIDAADALSAAGISEFEVNPMVLSKTG
                     ELVALDCLATLKEFAENAPANGSAQSSSQNLIQSLVRDSEGFPINFAQHIRPVNQIKH
                     ILEPSSVAIIGVSEKTINNGRIILRNLLENNFDKSRIYIIKPGAQWIDGCQCVPDVQS
                     LPEKIDLFVVVIPALGIPSTLADIARYDKAWSVLLIPGGLEEKQGSESIVADMNRALA
                     EARAEGKGPLINGGNCLGIRSVPGKYNTLFIPEYKLPMPKGKIEPLALISQSGAFAIT
                     RISKHPNINAKYAITIGNQMDLTVGDYLDYLAQDNELHVFAVYSEGFKPLDGQKALEA
                     TKEITMSGRNVILYRGGRTRAGAGAAASHTASIAGDYPVTWQLFSQAGAVVCDSLDEF
                     DDAITLFTLLDGKRAKGRRLAAVSNGGYECVAIADNLGEMVLSHFSERTKTELEVIYK
                     DAHISEIVDIHNPLDLTPMADDDAYERAVRTVLMDPETDLCIVGIIPLTAKLNTLAGG
                     PLSHGEDILRPNSLPERLGRLSSEMDKPFIAIVDSGPLYDPFSAELEKRGIPTFRAAD
                     RALRMLERWRKAHQRI"
     gene            complement(2468..3070)
                     /locus_tag="PK105_07755"
     CDS             complement(2468..3070)
                     /locus_tag="PK105_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOU61647.1"
                     /translation="MAKESEEIMTCDIILCSVGGQGGLSASIIIARAAMAAGFQVKQS
                     EIHGMSQRGGEVLAHLRIADKEIYSPTISKGKADIMFALEPLEALRHLSWISPSNGII
                     VSATAPLLNIPDYPDLDTVLAEIRKIARVRLIDAEAIARKSGNVRTANLVLIGAAMDL
                     LPISPDAIEKEIIELFSRKGESVVQANLKAFEAGRALYHG"
     gene            complement(3043..4686)
                     /locus_tag="PK105_07760"
     CDS             complement(3043..4686)
                     /locus_tag="PK105_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU61648.1"
                     /translation="MSQKVLLSGDEAIGQAAMDSGITAAFAYPGTPATEIMEYLQECI
                     SNTDNDESIGARDFRPVAQWCTNEKTAYESALGVSFAGRRTMVSMKHVGLNVAMDPFV
                     NSALVRINGGLVVIVADDPGMHSSQNEQDSRLLADFARIPCFEPSDQQEAYDMVRAAF
                     DYSEAHEVPVMVRIVTRLAHARGLVQTAPSVPICNRGKTTDRFSWTLLPASARKQWLA
                     LLAKQPAFKVDGEKASRLELRSKELGVVTCGLALAYYKENEGDWTVAHGGEPPSHLHL
                     GRYPIEVSEGESKVRALAAHVSQILVIEEGYPYIEREMRGIFGAPIPILGKMTGELPL
                     AGELSSDSVRIALGLPLVSSSLHSEIELPSRPPQFCQGCPHADSYTALKKALEGEAEF
                     FVFSDIGCYALGAQQPWNIIESCVDMGASVGMARGAGCVGNKRSIAVIGDSTFYHSGL
                     TNLLDAVQHHSPMTLMILDNGTTGMTGAQPTISPSQQLPKLLEGFGVEREHIRVLEAH
                     RRALDANVEIIREELSYDGVSVIIMFRECIEWLKKARRS"
     gene            complement(4775..>5184)
                     /locus_tag="PK105_07765"
     CDS             complement(4775..>5184)
                     /locus_tag="PK105_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOU61649.1"
                     /translation="GYEAPVSATFAHGSRAAAVRIPGYLGKGEARIEFRTGDATANVY
                     YFLSAMLLAGLDGINNRLDPVALGFARETTSARHVFPTGLFDVLAGLKKDHGYLSAAF
                     SEELVETWIAHKEKEASYVYNAPVPQEYELYFD"
BASE COUNT         1280 a         1349 c         1229 g         1326 t
ORIGIN      
        1 aatagccgat atctcttgca ccttgccgct ctatattctt tgatgggctt ttcgccagcg
       61 ctccagcatt cgcaaggctc ggtctgcggc gcgaaaagtt ggaatccctc gtttttcgag
      121 ctccgcggaa aaaggatcgt agagcggacc agaatcgaca atggcaataa aaggcttatc
      181 catttcgctc gataatctgc cgagccgttc gggaagcgaa ttaggtctga ggatatcttc
      241 gccatgagat aatgggccgc ctgcaagcgt attcaactta gcggtgaggg ggattattcc
      301 cacaatgcag agatcggttt cggggtccat aagtactgtt ctaaccgccc tttcataggc
      361 gtcgtcgtcc gccattgggg tgagatcgag tgggttgtgg atatctacta tttccgatat
      421 atgcgcatcc ttataaatca cttcaagctc ggtttttgtc ctttcactaa aatgagataa
      481 aaccatctcc cccagattgt ctgcaatcgc aacgcactca tagccgccgt tggagacggc
      541 tgcgagcctt ctaccttttg ctcttttacc atcgagcagg gtaaataatg tgattgcgtc
      601 gtcaaattcg tccagactgt cgcaaacaac cgcgccggct tgagaaaaga gttgccaagt
      661 aacaggatag tctcccgcaa tgcttgcagt atgactcgct gccgcacccg ctcctgctcg
      721 agtcctgcct cctcggtata gaataacatt gcgaccggac atggtaatct ctttggtcgc
      781 ttcgagtgcc ttctgtccat ccagtggctt aaaaccttca gaatagaccg caaatacatg
      841 gagttcgtta tcttgagcca gataatccag ataatctccg actgttaggt ccatctggtt
      901 gccaatggtt attgcatatt ttgcattgat gttgggatgc ttagagatgc gcgttatggc
      961 aaatgcgcct gactgtgaga tcagcgcaag tggctcgatc ttgcccttgg gcatgggtag
     1021 cttgtattca ggtatgaaga gggtgttgta tttaccggga accgagcgta taccaaggca
     1081 gttgccacca tttatcaggg gtccctttcc ctcggcgcgt gcttccgcca aagcacgatt
     1141 catatccgca actatggatt ctgagccttg cttttcttcc agccctccgg gaattaggag
     1201 aaccgaccat gctttgtcgt atcgcgcaat gtcggcaagc gtgctgggaa taccaagcgc
     1261 tggaataacc acaacaaaga gatctatttt ctcaggcaga gattgaacat ccggaacaca
     1321 ttggcaacca tcgatccatt gagcacctgg ctttatgata tagatcctag atttatcgaa
     1381 gttattttca aggagattgc ggagaataat acgaccattg ttgatcgtct tttcagatac
     1441 gccaatgata gccacgctag aaggctcgag aatgtgcttt atctggttga ctggccgaat
     1501 atgctgagcg aaattgatag gaaaaccctc ggaatcgcgc acaaggctct gaataaggtt
     1561 ctgtgaagaa ctctgcgcag agccatttgc tggcgcgttc tctgcaaact cttttagcgt
     1621 tgccaggcag tccagtgcta caagctcccc ggtttttgaa agcaccattg gattgacctc
     1681 gaattcgctt atgcctgcgg ccgaaagggc atctgcggca tcgataaagc gctggattgc
     1741 ttcgatgaga ctcttttctt ccaactctgg ccttgtattt cttaggccag cacataagag
     1801 cccatatatt acattgtttc ttagaagcga ttcgagcact gccatgtcat tgattcttgg
     1861 agagagaaaa aggtttgcca taccgggctt gaatttcgat gcgagatact cggtataaat
     1921 accaccaggg ccgaaagtca caatagggcc aaaatcaggc gtaaaacgat aaccaagaat
     1981 catctcatga ccaagcttcg gttcgaaagc tatgaactca ttgacagtaa atccatcgcg
     2041 aggcacattc ttgaatcgag cttccattct atcgagcgaa tcggcaattg cggtagccgt
     2101 gttatcgaca atctcgatgc cttgtacttc ggtcttatga agaaccttag aactaatgac
     2161 cttgattaca gctttggagc cggggaaagg gctttgcaga ggagcatcta ttgctccttc
     2221 agctcctatt ttctccagaa actcatttgc cgactgcaca atccaacgcc ggggcgtctg
     2281 tacacccatt gcggccagaa tggacattcc ttcgagctcc gtaagcgctt ccctatgctc
     2341 gctttgggct tgggatatta tgcgctttat cttgtcgcga agctcctttg ccgattgact
     2401 caggcgacgg ccgccttgag catattgagt tgtcgcctga tctttggtct tttgggtctt
     2461 ttgtctttca accatgatat agcgctctcc ctgcttcaaa agcctttaga ttggcttgta
     2521 caaccgattc gccttttctt gaaaagagct cgattatctc cttctcgatg gcatcaggag
     2581 aaatgggcaa aagatccata gcggcaccga tcagtacaag gtttgcagtt cgaacatttc
     2641 ccgatttccg cgctatggct tcagcatcga taagccgtac gcgagcgatt ttgcggattt
     2701 ctgccaacac ggtatccaga tcggggtaat caggaatgtt gagaaggggt gcagtggctg
     2761 agacaataat accattagag ggggaaatcc acgaaagatg acggagcgct tccaggggct
     2821 ctagagcaaa cataatatcc gctttgccct tggaaatcgt gggactatag atctccttgt
     2881 ctgcaatgcg caggtgagcc aaaacttcac ctccgcgctg agacatccca tgtatttcgg
     2941 attgcttaac ctgaaatcca gcggccattg cggcgcgggc tattatgatc gacgcagaaa
     3001 gcccgccctg gcctccgacg ctgcagagaa ttatatcgca ggtcatgatc tcctcgcttt
     3061 ctttagccat tcgatgcatt ctcggaacat tatgattaca gacacaccat cataacttag
     3121 ctcttcgcga atgatctcaa cattagcatc caatgcacga cgatgtgcct cgagaacacg
     3181 gatatgctcc ctttcaacac caaaaccttc gagaagcttt gggagttgtt ggcttggact
     3241 gattgtgggc tgggctccgg tcatgccagt agtgccattg tccaagatca tcaaggtcat
     3301 cggactatgg tgctgcaccg catcgagcaa gtttgtaagg cctgaatgat aaaaagtcga
     3361 atcgcctatt acggctatgg agcgcttgtt tccaacacag cctgcaccgc gcgccatacc
     3421 caccgaagcg cccatgtcta cgcaggactc tatgatattc caaggttgct gtgcaccaag
     3481 agcatagcaa ccgatatccg aaaagacaaa gaattctgcc tctccttcga gagccttctt
     3541 gagcgctgta taggaatcgg catgtggaca accctgacag aattggggtg gacggctagg
     3601 aagctcgatc tctgagtgca ggctggagga gactaaagga agtccaagag ctattcgcac
     3661 cgaatctgag ctcaactcgc ccgctagagg caattctccc gtcattttcc ccaaaatcgg
     3721 tatgggtgct ccgaatatgc ctcgcatttc gcgctcaatg taagggtagc cctcttcgat
     3781 taccagaatt tgcgacacat gcgcagcaag cgctctaacc tttgattcgc cttccgaaac
     3841 ctctattgga tatcggccta agtgtaggtg tgaaggcggc tcgccgccat gagccacggt
     3901 ccagtctccc tcattttcct tataatacgc cagagccagc ccacaggtca ccacaccaag
     3961 ttccttcgac cgcagctcaa ggcggctcgc cttttctcca tctaccttga aggcgggttg
     4021 ttttgctagc aaggccaacc actgctttcg cgccgaagca ggcaataggg tccaagaaaa
     4081 acgatcggtg gtcttgccac ggttgcagat aggcactgaa ggtgctgtct gtaccagacc
     4141 tcgggcatgc gcaagccttg tcactatgcg aaccatcacc ggcacctcat gcgcctccga
     4201 atagtcgaat gctgctcgca ccatatcgta ggcttcctgc tgatccgatg gttcaaaaca
     4261 aggaatgcga gcaaaatctg ccaaaagtct cgagtcttgt tcattttggc tggaatgcat
     4321 acctggatcg tcggccacta tgacaacgag ccccccattt attctaacga gtgcagaatt
     4381 cacaaaagga tccatcgcaa cattgagtcc aacatgcttc atagaaacca tggtgcgcct
     4441 acccgcgaag gatacgccta gtgcggactc gtatgcagtc ttctcattag tgcaccattg
     4501 ggctacaggc cggaagtctc tcgcaccaat gctttcatca ttgtctgtgt tgcttatgca
     4561 ttcctgcaaa tattccatga tctcagttgc cggtgttcct ggatacgcaa aggccgctgt
     4621 aattcccgaa tccatcgcgg cctgtcctat agcttcatct ccagaaagaa ggaccttttg
     4681 tgacatttcc gaactcctta ttcatccagt cagacaacaa tccaagaaaa taaaaaaaag
     4741 cgcaggaaca aacctgcgcc aacaatctcc ctgtctaatc aaagtatagc tcatactcct
     4801 gtggaacagg agcgttatac acatatgagg cttctttttc cttgtgcgct atccaggttt
     4861 cgacaagctc ctcggaaaac gcggccgaaa gataaccatg atccttcttg agcccggcta
     4921 acacatcaaa aagcccagtt gggaagacat gccgagccga agttgtttcg cgcgcaaagc
     4981 ctagagctac gggatcgagt ctgttattta tcccatcgag accggcgaga agcattgcgg
     5041 ataaaaaata atatacattt gccgtggcat cccctgtgcg aaactctatg cgagcttctc
     5101 cctttcctag atagccagga attcgaaccg ctgctgccct cgacccatgg gcaaaggttg
     5161 cgctaaccgg cgcctcataa cctg
//
LOCUS       DAOOWY010000119         5177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_16165_length_5177_cov_7.446505, whole genome shotgun sequence.
ACCESSION   DAOOWY010000119 DAOOWY010000000
VERSION     DAOOWY010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16165_length_5177_cov_7.446505"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..2313
                     /locus_tag="PK105_07770"
     CDS             316..2313
                     /locus_tag="PK105_07770"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOU61650.1"
                     /translation="MGSSLSRKIIESHLVEGSWKPGEEIGIKIDQTLTQDATGTMAYL
                     EFETLNIERARTELSVSYIDHNTLQIGFENADDHAYLTSIAQKYGILLSRAGNGICHQ
                     VHLERFGVPGKTLLGSDSHTPTSGGLSMLAIGAGGLDVALAMAGEPFFFVCPHVVRIF
                     LKGSLGKWVSAKDIALKVLEIFGTKGNVGTIFEYTGPGIITLSVPERATIANMGAECG
                     VTTTIFPSDDQTKAFLDLQGRLEAYIPLSADYDAAYDDSIEIDLDELEPLAAVPHSPG
                     NVKPVREIEGIKVDQVCIGSCTNSSYRDLAIAAQILKGKTIAPDLSLVVAPGSRQVLR
                     SIARDGYLDMLLAAGARLVEPSCSFCIGNGHSPASGAVSLRTSNRNFEGRSGTKDAQV
                     YLVSPETAAYAALLGTIRDPRTLEIDPTSISAHARPLIDDNLILKPLPASLAAAISIR
                     RGPNIGVPPTLHPLSERIDGLVAIKVGDKITTDHIMPAGDRMKYRSNIPAYAHYVFEP
                     IDPNFAVRAQAARDNGDDLIIVAGSSYGQGSSREHAAICPAFLGVRAVLAKSFERIHT
                     ANLINFGILPLLFDDETDYDKIQIGDRFQIENLRWIIGNEQLPLNFIVKDISQGIEIP
                     VHSMLTPRQRKMLLAGGALGLVSSGQFSIHSASTPNLGAQA"
     gene            2379..3524
                     /locus_tag="PK105_07775"
     CDS             2379..3524
                     /locus_tag="PK105_07775"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOU61651.1"
                     /translation="MIKEKLILPNVDLQLEYYDLGIQHRDETDDQVTLDAARAIAHHK
                     VGVKCATITPDAERVKEYNLKKPWPSPNATIRAYLDGTVFRKPILARNIIPAVRSWQK
                     PIIIGRHAYGDIYKAVDFVVPGPGSVSLMYQSSRGGQPLVWKLHDFESQGVVMGMYNI
                     DSSIRSFARACIEYALSEKCDIWFAAKDTISKNYHGHFKELFTEEVAARVADLDAQGI
                     HYRYMLIDDAVAQLMKSAGGMLWACMNYDGDIFSDMIAAGFGSLGMMSSVLVSPSGSF
                     EYEAAHGTVRRHYYEHLKGNPTSTNPIASLFAWTGALKKRGELDRTPEISEFAAQLET
                     TAIELIEAGIVTKDLMNLVNPPAKAFETTESFLDKIAALLTTTRSST"
     gene            3619..4623
                     /locus_tag="PK105_07780"
     CDS             3619..4623
                     /locus_tag="PK105_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOU61652.1"
                     /translation="MMKTDSLTIIDNRTGQSYELPITDGTIRASDFRSIKTDENDFGL
                     MSYDPGFTNTATARSAITYTDGEKGILRYRGYPIEQLAERSTYLETAYLIIFGELPTK
                     KQLDEWTTTINDHVMIHTNIQNLIETFRYDAHPMSIFISAVAALATLYPESRNVTDPE
                     IRRMQAACLIAKTPSIAAFIYRHNMGWPIRYPDEELSFIGNFLKMMFQMKGTHYKPHP
                     VLEKALNVLFILHADHEQNCGTTAMRTIGSSHADPYTALAGAAAALYGPLHGAANEMV
                     MRQLEEIGDPNRVKDYVEKVKRGEIRLMGFGHRVYKNYDPRCRIIKKNSGRGTFGDRH
                     "
     gene            4610..4918
                     /locus_tag="PK105_07785"
     CDS             4610..4918
                     /locus_tag="PK105_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOU61653.1"
                     /translation="MTGTNKLFDVALELERIALQDEYFISRKLYPNVHFYSGLVYSAM
                     GLHSPLFSVMFAVPRTSGWVAHWMELFDDQEQKLARPRQIYVGEQKREYLSLEDRSVQ
                     "
BASE COUNT         1467 a         1169 c         1163 g         1378 t
ORIGIN      
        1 tattacccca gccttccaag ctggagacgt gggttcgact cccatcggtc gcttttcgtt
       61 ctgttaccct ttttgcagat acttttttat aactttcttg taactttctt ataattgccc
      121 cttgtgcttc ttttttctgc cggcgaagag acggaattcc acactcatta aaactgataa
      181 gatcatcaca ctgtcatgac gctatatagg attcaaaatt gaattcaaaa aataataaaa
      241 acaatcttgc aaagtgccgt gaaattatgt atatatatgc atcttggttt tatgtatatg
      301 tagggaggca tggctatggg aagttcacta tcacgcaaga ttattgaatc tcatcttgta
      361 gaaggatcat ggaagcccgg cgaagaaata ggtataaaaa tcgatcagac ccttacccag
      421 gatgcaactg gcacaatggc ataccttgaa tttgagacct tgaatattga gagagcaaga
      481 acagagcttt cagtgagtta tatcgatcat aataccctac aaataggctt tgaaaatgct
      541 gatgatcatg cctacctcac cagtattgct cagaaatatg gcatcttgct ttctcgagca
      601 ggcaatggaa tttgtcatca agttcatctt gaacgctttg gagtaccagg caaaactttg
      661 ctcggctcag atagccacac acccacgtca ggagggctta gtatgctcgc aatcggcgcc
      721 ggaggacttg atgttgcact tgcaatggct ggagagcctt ttttctttgt gtgcccccat
      781 gtggtgcgga tttttctgaa aggtagcctt ggcaagtggg tttctgcaaa ggatattgca
      841 ctcaaagtat tagaaatttt cggtaccaaa ggcaatgtcg gcacaatttt tgaatatact
      901 ggacctggca ttatcactct ttctgttcct gagcgagcaa cgattgccaa catgggcgct
      961 gaatgcggcg tgacgaccac tattttcccg agcgatgacc aaacaaaggc ctttttagat
     1021 ttgcaaggcc gtctagaagc ctatattcca ctcagtgcag attacgatgc agcgtatgat
     1081 gattctattg aaatcgattt ggacgaactt gaaccacttg ccgcagtacc tcattcgccg
     1141 ggcaatgtca agcctgtgag agaaattgag ggaataaaag ttgatcaggt atgcattgga
     1201 tcatgtacaa attcctcgta tcgcgatcta gcgattgctg cgcaaattct aaaaggaaag
     1261 accattgcgc ctgacctgag ccttgttgta gcgccaggat cgcgtcaagt gctccgttcg
     1321 attgctcgag atggctacct cgacatgctg cttgctgcag gagcgcggct tgttgagcca
     1381 tcgtgtagtt tctgtattgg caatggccat tctcccgcaa gcggcgcagt ttctctgcgt
     1441 acttcaaatc gcaattttga gggaagatcg ggaacaaaag atgctcaagt ttacctggta
     1501 agtccagaaa cggctgcgta tgcagcactc ttaggaacaa tcagagatcc tcgaacgctt
     1561 gagattgatc caacatcgat atctgcgcat gcaaggccac tcatcgatga taaccttatc
     1621 cttaagcctt tgcctgcatc gttggctgca gcgatttcaa tacgccgagg accgaatatt
     1681 ggcgtaccac ccacccttca tccactctct gagcgcatcg atggattggt agcgattaaa
     1741 gttggcgata aaattaccac cgatcacatt atgcctgcgg gcgatcgcat gaaataccgc
     1801 agcaatatac cagcctatgc tcattatgtt tttgaaccaa tcgatccaaa ctttgctgtt
     1861 cgagcccaag ctgcacgcga caacggagat gatctcatca tcgtcgctgg ttcttcatat
     1921 ggccaaggct catcgagaga gcatgcagca atatgtcccg cgttccttgg agttcgcgca
     1981 gtacttgcga aatcattcga gcgcattcac acagccaatc tcataaactt tggcattttg
     2041 ccacttcttt ttgatgatga aactgattat gacaagatac agattggtga tagattccaa
     2101 attgaaaatc tccgctggat tattggaaat gagcaacttc cgttgaattt tattgtaaaa
     2161 gatatatcgc aaggcataga gataccggtg catagcatgc tcactcctcg acaaaggaaa
     2221 atgcttctcg caggaggagc gcttggtcta gtttcttctg ggcagttttc aattcattct
     2281 gcatctacac caaatctagg agcacaggca tgaaactgaa agtaaagaat ccgattgtag
     2341 agcttgatgg cgatgaaatg gcgagaattc tttagtccat gataaaagaa aaactcattc
     2401 tccctaatgt ggaccttcag cttgaatatt acgaccttgg tatccagcat cgagatgaaa
     2461 ccgacgacca ggtaacgctc gatgcagccc gtgcaattgc gcaccacaaa gtgggtgtaa
     2521 aatgtgcaac tattacgcct gatgccgaaa gagtcaaaga atataatcta aaaaaaccat
     2581 ggccttcccc caatgctaca attcgagcat atctcgacgg cacagtgttt cgaaaaccta
     2641 ttcttgcaag aaatattatt ccagcggtac gcagttggca aaagccgatc atcattggta
     2701 ggcatgccta tggcgatatc tataaggcag tggattttgt ggtgcctggc ccgggttcgg
     2761 tgagcctcat gtatcaatcg tcaagaggtg gccagcctct tgtttggaag cttcacgatt
     2821 ttgaaagcca gggtgtggtg atgggcatgt acaacatcga tagctcaatt cgctcttttg
     2881 cccgtgcctg catagaatat gcgctctcag aaaaatgcga tatttggttt gctgccaagg
     2941 atactattag caaaaattac catggccact ttaaggagct ttttactgag gaagtcgccg
     3001 cgcgcgtggc cgacctcgat gcccaaggaa tccactatcg ttacatgctc atcgacgatg
     3061 cagttgctca attgatgaaa agcgcaggag gaatgctctg ggcatgcatg aactatgatg
     3121 gcgatatctt ttcagatatg atcgctgcag gatttgggag ccttggcatg atgagctcag
     3181 tccttgtttc tccttctggc tcatttgagt atgaagcagc gcatggtact gtgcggcgac
     3241 attattatga acatttgaaa gggaatccta ccagcactaa ccctatcgct tctctctttg
     3301 cctggacagg ggcgctcaaa aagcgtggag aactcgacag aacacccgaa atatctgaat
     3361 ttgcagcaca gctcgaaact acagcgatcg agctcattga agcaggtatt gtcacaaaag
     3421 acctcatgaa tcttgtgaac cctccagcca aagcttttga aaccaccgaa tcattcctcg
     3481 ataaaattgc tgcattactc accaccacac gaagcagtac ataataattg caacaattat
     3541 ccttgccttt tccagggagg ggctaatttt ttctgtttta atatgaagga tattgaagga
     3601 tattatagaa ggagccacat gatgaagaca gattcattaa caataatcga taatagaaca
     3661 ggccaatcat atgaactacc tattacagat ggaactattc gcgcttcaga ttttcggtcc
     3721 ataaagaccg atgaaaatga ctttggcctc atgtcctatg atccaggatt tacaaatacc
     3781 gcgactgctc gcagcgcaat tacgtatact gatggagaaa aaggtatcct ccgttatcga
     3841 ggctatccaa tagagcagct tgctgagcga tccacctatc tcgagacagc ctatctcatc
     3901 atctttggtg agctaccaac taaaaaacaa ctcgatgaat ggactaccac catcaacgac
     3961 catgtcatga ttcataccaa cattcaaaac ctcatcgaaa catttcgata tgacgctcat
     4021 ccaatgagca tctttattag cgctgttgca gctctcgcta ctctctatcc agaatctcga
     4081 aatgtcaccg atccagaaat tcgacgaatg caagcggcat gccttattgc aaaaacacca
     4141 tcaattgctg cattcatcta tcgccataac atgggatggc cgattcgcta tccagatgag
     4201 gagctttcat tcatcggcaa ttttctcaag atgatgtttc aaatgaaagg cacgcactat
     4261 aaaccacatc cagtgctcga aaaagcgctc aatgtgctgt ttattcttca tgccgatcat
     4321 gaacagaatt gtgggactac tgcaatgcgt accattggtt cctcccatgc cgatccctat
     4381 acagcacttg caggagctgc agcagcgctc tatggcccat tgcatggtgc agcaaacgaa
     4441 atggtaatgc gacaacttga agaaattggc gaccccaacc gagtcaagga ttatgtagaa
     4501 aaagtaaaac gtggcgagat tcgactcatg gggttcgggc atcgagtcta taagaattat
     4561 gatccgcgct gtcgtatcat caaaaaaaac agcggaagag gtactttcgg tgaccggcac
     4621 taataagctc tttgatgttg cgttggagct cgagcgaatt gctctacaag atgaatattt
     4681 tatatcgcgc aagctttatc cgaatgttca tttttattct ggacttgtct atagcgcgat
     4741 gggccttcat tccccgcttt tttcagtgat gttcgcagta ccgcggacct ccggctgggt
     4801 tgctcattgg atggaacttt tcgatgacca ggaacaaaaa cttgctcgac ctcgacaaat
     4861 ctatgtaggc gagcagaaaa gggagtatct ttcgctggaa gatcgaagtg tacagtagag
     4921 aatagagaag cgcgctctat agtgggccaa ttgtaaaagt ccaagcatat tttggaatgg
     4981 cttacaagaa tgggaaaata gggacgtaat ttcgcaggat aaaccatgga gccagtatca
     5041 ccacgtatat aaggctaagg caaaaaagat gactttgagg cgtttccagg aggcacaagc
     5101 gcatcgctct ctccgatacg tctggagggg gtatgataaa atggacatga agttaagaga
     5161 aaaaaggaag ggttcat
//
LOCUS       DAOOWY010000120         5161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_16223_length_5161_cov_12.008813, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000120 DAOOWY010000000
VERSION     DAOOWY010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16223_length_5161_cov_12.008813"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK105_07790"
     CDS             complement(<1..423)
                     /locus_tag="PK105_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU61654.1"
                     /translation="MNKRPYMDIGAIFDDIFEAAKDFGERVKNFSPGFGGPNQEGEES
                     RPGCHGMGGAWFEAHGDDNVDYYPNYSYPPANIYMLPDRSLIFEFALAGFDEKNLSLS
                     FQGDYMVFSARIEAEKPEEGVRYFKRRLRLKDIDKQKYY"
     gene            complement(664..2505)
                     /locus_tag="PK105_07795"
     CDS             complement(664..2505)
                     /locus_tag="PK105_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOU61655.1"
                     /translation="MKRFFMVLCALLAMTFTLSAQSKSVDLTFIETSDIHGSIYPYNF
                     ITAKPAGTSLAQVASLIKEERSVPDAEVVLLENGDSLQGQPTVYYYNFEKTDGPHIWS
                     QAVNFLNYDAVSVGNHDIEAGHAVYDKLYEELQAPVICANAIKPDGTPYFEPYAVIEK
                     AGIKIAILGMITPKIPDWLPPQFWTGMQFEDMVKCAQKWVPIIQQKEKPDLLIGLFHA
                     GVDYSYGNVGRDTPNNENASQLVAEMVPGFDLIFVGHDHQGWSGQGWDPANKAKVDVK
                     DPNGKIVPIYGALNAARNVAVVKMNLTWNDETQSWDKIVRGGLVDMSKYQPDPDFMTK
                     FQPMFDEIKAWVDRPIGKMEGVISSRDSMFGDSAFVDLIHIIQLELTKDPTLGLAPAD
                     VSFAAPLFADAQIPSSPDGTLYVRDMFNLYVYENFLYTMNLTGQQIKDFLEYSYQYWF
                     DTMPNEGNHLIAFQRDKEGKLILDNRTNMPMTATRYYNYDSAAGINYIVDVSKPAGER
                     VSIYCMSDGRAFNPKAKYTVAINSYRASGGGGHLEKGAGLDKDTILSMKLVNGATTKD
                     LRYFLLKWFEKQEETITVWPLGNWEVVPEDLAAIGRANDYPLLYPSK"
     gene            2754..4103
                     /locus_tag="PK105_07800"
     CDS             2754..4103
                     /locus_tag="PK105_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61656.1"
                     /translation="MRSSRYALFFALLFIGAILFGAAGQFASAQSASTQAQPVVSQKQ
                     DIAVFALGYYGWLIPSQALGTIDLKIQEVFAKIGRFNVFGTTQRLSSNGVQEFIDTLR
                     RSKEANFVLPEQYQFGEAFLTKAEFERLTGAFVVVVPVVTNFKVNWDSTSNAWVCNIT
                     TGITFIDAAHDAKVISIESIDTSGSDKKNPNDAVKAAIDAIPAELEYRIRSIPEFQIS
                     TRILEVSGSTVKIQLGSNMGLKKGDEYAIVEIRNVGGIQDTQETGLIMITDVSSQIST
                     GRILYKSGSVGQNTQLKEIAKYGSDLDLYFHMSGKEMLPGLRATIARGFFPFRPYVGI
                     QMPIDLAFNFFSVTAIPVNVAIGGEYMIPLGRLHVAPYVGAGITYLYITEAITGYTNT
                     NYLSHYGFQAGARIGYLINRNMRVFLDAGYDYWASLDSGLFPNYGGLTIGAGVTFKL"
     gene            complement(4110..>5161)
                     /locus_tag="PK105_07805"
     CDS             complement(4110..>5161)
                     /locus_tag="PK105_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOU61657.1"
                     /translation="ILFFCISFLEDTGYIARAAFLTDHFLHAIGLHGQSFMPLMLGFG
                     CSVPAIMATRTLKSPKERIATILAIPFISCGGKLPIYVLLAGTFFPNHESTVVMLVYA
                     TGVVLSILSTYFLRRTVLKGQSMPFVMELPPYRMPTLKGVLWHVWEKTWSYVRKAGTI
                     ILAVSVLMWVITSYPKAKGIDDYALQVRQEIATAHPDFDESLIAANVEILLRQYQLER
                     SIAGNIGKIIEPVIAPIGFNWKIGIALLPGLTAKELVVSTLGVLYGTTVNDETEANSS
                     LRQALRNSPDWSPRIALALLIFILVMPPCFSSLAVIRTEAGDKWLAFQVVYSLVLAWV
                     LSFLASMAGHLLGLG"
BASE COUNT         1382 a         1170 c         1224 g         1385 t
ORIGIN      
        1 atagtacttt tgtttgtcta tatcctttag cctaagtctt ctcttgaagt atcgcactcc
       61 ttcttccggt ttctcagcct cgatcctggc ggaaaagacc atataatctc cctggaaaga
      121 taggctcagg ttcttttcat cgaaacctgc caacgcgaat tcaaatatca ggcttctgtc
      181 aggcaacatg tatatattgg cgggcggata ggagtagttt gggtaataat ctacattatc
      241 gtctccgtga gcctcgaacc atgcaccacc catgccatgg caccccggcc ttgactcttc
      301 gccttcctga tttggacctc cgaagccagg tgagaaattc ttgactcttt cgccaaaatc
      361 tttggccgct tcgaaaatgt catcgaagat agctccaatg tccatgtaag gtctcttatt
      421 catttttcta tctcctttcg ggaatctttt gacgccgcgg cttatgcccc tcggctttgt
      481 gatagcttcc catgcgggca agctcttttc ttgatttcaa tatagaaata aagactcata
      541 aaatcaagaa gaagcatcga tactaaaaag ctatgattca atccataata aaatcaatcc
      601 ataataaaaa ggggctgccg cttaacttgg gcagcccctg cctgagctca aagcagacga
      661 tctctatttc gaaggataga gaagcggata atcattcgct ctgcctattg cggccaggtc
      721 ttctgggacg acttcccagt ttccgagagg ccataccgta atcgtttcct cctgcttctc
      781 gaaccacttt aggaggaaat atcgaaggtc ttttgttgtg gctccgttta cgagcttcat
      841 ggagagaatc gtgtctttat ccagacctgc acctttttcc agatgtccgc cgccgcctga
      901 tgcacgatag gaattgatcg caacagtata tttcgccttg gggttgaaag ctcggccatc
      961 ggacatacaa tagatcgata cacgctcgcc ggctggttta ctgacatcga ctatatagtt
     1021 gattccggca gccgaatcgt agttgtagta gcgagtggca gtcatgggca tattggttcg
     1081 attgtccagt ataagcttgc cctccttgtc tctctggaaa gctattagat ggttaccctc
     1141 gttgggcatg gtatcgaacc agtactggta ggagtattca agaaaatcct tgatctgttg
     1201 tcctgttaga ttcatggtgt ataggaagtt ctcgtagaca tagaggttga acatgtcgcg
     1261 cacatataaa gttccatccg ggctcgaagg aatttgagca tccgcaaaca gtggagcggc
     1321 aaaggaaaca tcggctggag caagcccgag tgtcgggtcc ttagtgagtt cgagctgaat
     1381 tatatggatt agatcgacaa aggccgaatc tccgaacatc gaatcgcgac tcgaaatgac
     1441 accttccatt ttgcctatag gtcggtcgac ccatgctttg atttcatcga acatgggctg
     1501 aaatttggtc ataaagtcag gatctggttg gtacttactc atatcgacaa gaccgccccg
     1561 gacgattttg tcccagcttt gcgtttcatc attccatgtt aggttcatct ttacaacagc
     1621 tacattgcgg gctgcattta gagctccata gataggcact atcttgccat taggatcttt
     1681 tacatcgacc tttgctttat tagccggatc ccagccctga ccagaccagc cttgatgatc
     1741 atgaccaaca aagatcagat cgaaaccagg taccatttcg gctactagct gcgaggcgtt
     1801 ttcattgttc ggagtatcgc gtccaacatt tccataggaa taatccacgc cagcatggaa
     1861 aaggccaata agaaggtcgg gtttttcttt ttgttgaatg atgggcaccc atttctgagc
     1921 gcacttgacc atgtcttcaa attgcatgcc ggtccagaac tgtgggggta gccagtctgg
     1981 gattttgggt gtgatcatgc ccagaattgc tatttttatt ccagcttttt ctataacagc
     2041 atagggttcg aaatagggcg ttccatcggg tttgatggcg tttgcacaga ttaccggcgc
     2101 ttgtagctcc tcatagagtt tgtcatagac tgcatgacca gcctcgatgt catggttgcc
     2161 tacactcaca gcatcgtagt tgagaaaatt caccgcttga gaccagatat gaggaccatc
     2221 ggtcttttca aagttatagt agtagactgt gggctgtccc tgaagagaat caccattttc
     2281 aagaagcacc acctcagcat ctggtaccga acgttcctct ttgatgaggc ttgccacctg
     2341 cgcaagcgat gttccggctg gctttgcggt aatgaaattg tacggataaa tcgagccatg
     2401 aatatcgctt gtctcgatga atgtcagatc gactgatttg ctctgagcgg ataatgtgaa
     2461 agtcatagca agcaatgcac agagcaccat aaagaatctt ttcataagac ctccttatct
     2521 atggtttttt tgagcataat aatgataagc ccatttcaag gagagcgcaa catgattttc
     2581 aagaatgaca ttcaagggtg agcagcatgg tgagcagcac ggtctcctgt aaagagaact
     2641 gtccacttaa ggtgagcagc accaataatt tgtttgactt ctctagagta cttatcctat
     2701 aattcatcat atagtttcgc ttaaaaggat tgcccaaaaa ggagggttat cgtatgagat
     2761 cgtctagata tgctctgttt tttgcattgc tattcattgg agcaatatta tttggtgcag
     2821 ctgggcaatt tgcaagtgcg cagtctgcga gtacacaagc acaacctgtc gtaagccaaa
     2881 aacaagacat tgctgtattt gctttgggct attatggatg gcttattcca tctcaggcat
     2941 tgggtacaat cgatctcaag attcaggaag tgttcgctaa gattggcaga ttcaatgttt
     3001 ttggaacaac acagcgactc tcatcgaacg gggtacaaga atttatcgat acactgcgca
     3061 ggtcaaaaga agccaatttc gtgcttccgg agcagtatca atttggtgag gcctttctta
     3121 caaaagcgga gttcgagcgt cttactggag ctttcgtcgt tgtggtgcct gttgtaacga
     3181 attttaaggt caattgggat tccacttcca acgcgtgggt atgcaacata accactggca
     3241 taactttcat cgatgcagca cacgatgcaa aggttatctc aatcgaatcg atcgatacct
     3301 cgggcagcga caagaagaat cctaatgatg cggttaaggc cgcaatagac gcgattccag
     3361 ctgagctcga atatagaatc agatcaattc ccgagttcca gatcagtacg cgaatattgg
     3421 aggtttcggg ttctacggtc aaaatccaac ttggcagcaa catgggactt aaaaagggcg
     3481 atgaatatgc cattgtagag atccggaatg ttggcggcat ccaggatacg caggaaacag
     3541 gactgataat gatcacagat gtaagcagcc agatttccac agggcgcata ctctataaga
     3601 gcggctcggt cggccagaat acgcagctga aagagattgc aaaatatggt tcagatctcg
     3661 atttgtattt tcacatgagt ggcaaagaga tgcttccggg tttgagagcg accatagcca
     3721 gggggttctt cccattcagg ccctatgtcg gtatacagat gccaatagat ctcgcattca
     3781 atttctttag cgtaacagca ataccagtaa atgtcgcgat tggtggcgaa tacatgattc
     3841 cgctcgggag gcttcatgtg gcaccatatg ttggtgctgg cattacatat ttgtatatta
     3901 cagaagctat tactggctat acgaacacaa attatctatc tcattatggc ttccaggcag
     3961 gtgcaaggat tggatatctg atcaacagga atatgcgtgt atttctagac gctggatatg
     4021 attattgggc atctcttgat tctggcttgt ttccaaatta cggagggcta acgattggtg
     4081 cgggagtgac attcaagctc taacagacct cagcccaatc ctaagaggtg ccctgccatc
     4141 gaggcaagga aggatagcac ccaagccagg acaagcgagt atacaacttg aaaagctagc
     4201 catttgtcac cggcttcggt gcgtattaca gccaaagatg aaaaacatgg tggcataacc
     4261 aggataaaaa tcagaagagc aagggctata cgcggggacc agtctggaga attgcgaaga
     4321 gcttggcgca gcgaagaatt cgcctctgtt tcatcattta ctgtcgtacc atagagtaca
     4381 cctaatgtag agaccacaag ttcctttgcc gttaggcctg gtaggagcgc aattccaatt
     4441 ttccaattga agccgatggg cgcaattacc ggctcgatta tcttgcctat gtttcccgcg
     4501 atactccgtt cgagctgata ttgacgaagg agtatctcaa catttgcagc aatcagcgat
     4561 tcatcaaaat caggatgagc ggtggcaatc tcctggcgca cttgcagcgc atagtcatca
     4621 atccccttgg cttttggata cgatgtaata acccacatga gtacagatac ggcgaggata
     4681 atcgtacccg ctttgcgcac atagctccat gtcttttccc agacatgcca gagcactcct
     4741 tttagcgtgg gcatgcgata gggcggcagc tccatgacaa atggcataga ttggcctttt
     4801 aacaccgttc gcctaagaaa ataggtcgaa agaatgctca aaacaactcc ggtggcgtac
     4861 accagcataa cgacagtcga ttcatgattt gggaaaaaag tcccggccag gagcacatag
     4921 attggcagct tcccgccgca ggaaataaag ggaattgcca gaatcgtggc gattcgctct
     4981 ttggggcttt tgagcgtacg ggtggccatt atagctggca ccgaacagcc aaacccaagc
     5041 atgagcggca tgaacgactg tccatgcagc cctattgcgt ggagaaaatg atcggtcaaa
     5101 aaggcagcgc gagcaatgta gcctgtatct tcaagaaagg agatgcagaa gaaaagaatc
     5161 a
//
LOCUS       DAOOWY010000121         5152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_16267_length_5152_cov_7.534040, whole genome shotgun sequence.
ACCESSION   DAOOWY010000121 DAOOWY010000000
VERSION     DAOOWY010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16267_length_5152_cov_7.534040"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK105_07810"
     CDS             complement(<1..396)
                     /locus_tag="PK105_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU61658.1"
                     /translation="MSETKNPFPDRFYSLQVEEKMKMLETMIRIRTFETHVEELFMNK
                     KIFGFVHLYIGEEAIATGVMANLRVDDYITSTHRGHGHAIAKGANTSRMMAELFGRKT
                     GYCKGKGGSMHIADFSIGMLGANGVVGGGF"
     gene            complement(414..1706)
                     /locus_tag="PK105_07815"
     CDS             complement(414..1706)
                     /locus_tag="PK105_07815"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU61659.1"
                     /translation="MAALLVFGVFIFLIIIGVPIASSLGTAAIGAIMNYDLGMSMISR
                     NFEANIAKFPLVAIPFFILAGVLMDKSKIVDGIARFLILLTGRRRGGLAIAAVIACIF
                     WGAVSGSGPATAAALGLVFITPMIKQGYNKYFAATTIAAGAGLSIIIPPSISFIVYGQ
                     LTSVSVGALFLGGIIPGVLVGISLIGVVYIISRKNNYRGAEESGTPKQIFKAFIDSLW
                     ALFAPVIILGSIYLGIATPTEAAIMGVFYALFVGIFVYHTLPINVIYQALVDTVVSSA
                     VVMFVVSMAGIFSWAAATLGVIDAAANLISSITTNKLIFMFLSMIVMLIAGMFLDAIS
                     ITNVFMPMIMPALLALHIDPLYFGVCLTAALAIGQITPPVAVNLYVTANLIHSNINNE
                     MVKFVLPMVYASVLALILIVLIPQLSLFIPIISKLYIP"
     gene            complement(1706..2221)
                     /locus_tag="PK105_07820"
     CDS             complement(1706..2221)
                     /locus_tag="PK105_07820"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOU61660.1"
                     /translation="MKKKRICIDELMGAIFLAIMAIIAFTNVINRYFFHRGFAFTEEI
                     TVNLFVWITLLGIAIAFRRGANLKMTNLFDTFSPNLKRVALIISTCLGVMIFCFLIYN
                     SAREIYKNITFYHAFSESLGIPTWIYSLGTPVFSIFVIKEIIASGIREIKSLKAPLPS
                     GNSENSKEEAQ"
     gene            complement(2312..3364)
                     /locus_tag="PK105_07825"
     CDS             complement(2312..3364)
                     /locus_tag="PK105_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOU61661.1"
                     /translation="MIYRRFMLLLVALLIAILPVTAFTPKREYTMQVNVTQAVDWGAG
                     AQKFADLVAQKTNGVINIKPYWSSSLLAGKQTNWYQAVADGAIDFATESTMNACTVIR
                     PFNLFSLPFFINTYDNVDKIENGKAGARMIAEMERGGVKFLAWGENGFRQITNSKREI
                     RTPKDVEGLKIRVVGSPIMIDIFKQLGADPINMNWGDAVTAFQQGVVDGQENPKGVLI
                     GNKIWEYHKYMTIWNYLIDPLVFCVNSQVWKSFPPDIQAQIQEAALEAGEWEKALARA
                     YLDGDKSLKIFKEKFKDPRDPENLFVIAPSKGMKITVLTAQEIDAFKEKTKPVYDKWI
                     PTIGEDIYKLALEDMK"
     gene            3661..4938
                     /locus_tag="PK105_07830"
     CDS             3661..4938
                     /locus_tag="PK105_07830"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOU61662.1"
                     /translation="MKKKIVLAYSGGLDTTVIVPWLKEHYDCEVIAVCGDVGQEADFD
                     GIGARALASGASKFIKLDQKEEFVKEYLWALVKAGTPYEKKYLLGTSAARPLLAKGLT
                     EVALAEGADAVAHGCTGKGNDQVRFELGVKAFAPEMEVIAPWRIWDIQSREEEIAYLE
                     ARHIPVPMKKSDSYSRDDNLWHISHEGLDLEDPSNEPYYEGMLKMCVTPEKAPDKPEY
                     VTLGFEQGVPVSINGEKLDPVSIVRKLNKIGGANGVGIVDMVENRVVGMKSRGVYETP
                     GGTLIMEAHDRLEQLCLDKKTLSFKMTVAQRFAEILYEGEWFSPLCTALRAFIDSTQD
                     VISGQVTLKLYKGNMVFAGSKSSYSLYDASLASFTTGPLFSHSDSTGFINLFGLPTKV
                     RARLNERIKKYGQAASPDVVKPGNSGYTAHAGD"
     gene            4945..>5152
                     /locus_tag="PK105_07835"
     CDS             4945..>5152
                     /locus_tag="PK105_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOU61663.1"
                     /translation="MGKLWEAGEDGELDPVIEQFLSSIKADVRLIFEDIECSQAHAIM
                     LGEQGIISLQDASLLVDELAKIRKE"
BASE COUNT         1456 a         1142 c         1184 g         1370 t
ORIGIN      
        1 gaatccaccg cctacaacac catttgctcc aagcatgccg atagagaaat cggcgatatg
       61 catcgagcct cctttgcctt tgcagtagcc agtcttcctg ccgaacaatt cagccatcat
      121 tctgctggta tttgcgcctt ttgcaatggc atggccatgg ccacggtgcg ttgaggtgat
      181 atagtcatcg acgcgcaggt ttgccatgac tccagttgct atagcttctt ctcctatata
      241 gagatggaca aagccgaaga ttttcttatt catgaagagc tcttcgacat gagtttcgaa
      301 tgtccttatg cgaatcattg tttcgagcat cttcatcttt tcttcgacct gtaaagaata
      361 aaagcgatca ggaaagggat tttttgtttc tgacattgag cacctcctca tgctcatggt
      421 atgtatagtt tagagataat gggaataaac agagaaagtt gtggtatcaa cacgatcaga
      481 atgagcgcaa gaacagaggc atacaccata ggaagcacaa atttgaccat ctcgttgttt
      541 atattgctat ggatgagatt agcagtgacg tacagattga ctgccactgg aggtgtaatc
      601 tgaccaattg caagcgctgc cgtaaggcaa acgccaaaat agagtggatc gatatggagc
      661 gcaagcaaag caggcattat cattggcata aatacattag ttattgagat tgcatccagg
      721 aacattccgg caatgagcat gactatcatt gaaaggaaca taaaaattag cttattcgtg
      781 gtgatgctcg atatgagatt ggcggccgca tcgatgacgc cgagcgtagc cgcagcccag
      841 gaaaatatgc cagccatcga gaccacgaac atgaccaccg cagaggaaac tacggtatcg
      901 accaaggcct gataaatcac attgattggc aatgtatgat agacgaatat cccaacaaat
      961 agggcataaa atacgcccat tatggctgct tccgtaggcg tggctattcc caaatatatc
     1021 gaaccgagaa ttattacagg agcaaaaagc gcccaaagcg aatcgataaa agccttaaat
     1081 atttgctttg gagtgccgga ttcttccgct cctcggtaat tgttctttct agatattata
     1141 tagactactc ctataaggga aattcctact aacactccag gaataatgcc acccaagaac
     1201 aaagctccga cagatacact ggtcagctgg ccatagacta taaaagaaat cgaagggggt
     1261 atgatgatcg aaagccccgc acccgcagcg atcgtagttg ctgcaaaata tttattgtag
     1321 ccctgcttta tcatgggagt tataaataca agtccaaggg cagcggcggt cgctggcccc
     1381 gagcccgaga cagctcccca aaaaatgcag gctattaccg cggcaattgc caatccaccg
     1441 cgccgtctcc ctgtgagcaa tatcaaaaag cgtgcaatgc catctactat ttttgatttg
     1501 tccatgagca ccccggcaag tataaaaaat gggattgcga caagagggaa tttggcaata
     1561 ttggcctcga aattccgaga tatcatggac attccaagat catagttcat tattgctccg
     1621 atggccgctg taccaagcga tgaagcgatc ggaaccccta taataataag gaagataaaa
     1681 actccgaata ctaaaagcgc tgccattatt gagcctcctc tttggaattt tctgaattgc
     1741 ctgaaggaag aggggctttg agggatttta tttctctaat accgcttgcg atgatttctt
     1801 ttatgacaaa tatcgaaaac acaggcgtcc caaggctata tatccatgtt ggaataccaa
     1861 gagattccga aaatgcgtga tagaaagtta tatttttgta gatttctcgc gcagaattat
     1921 aaatcagaaa acaaaatatc attactccga ggcatgtgga tattatcaat gccacacgct
     1981 ttagattggg cgagaatgtg tcaaataagt tggtcatctt tagatttgca ccgcgccgaa
     2041 aggcaattgc gattcctaag agcgtgatcc atacaaagag attgacagtg atttcctctg
     2101 taaatgcaaa acctctatga aagaaatatc ggttgataac attggtgaaa gctatgatcg
     2161 ccataatcgc tagaaaaata gcgcccataa gttcatcgat gcatatcctt ttcttcttca
     2221 ttatggttct ccttagagaa aagccggcca tagaggccgg ccaagaatag aaggtactat
     2281 acggtacctg tatgttgcgg cgtaaggcac gttatttcat atcttcgagc gcgagtttat
     2341 agatatcttc gccgattgtg ggtatccact tatcatatac aggttttgtt ttttctttga
     2401 atgcgtctat ctcttgagcg gtaagaacgg tgatcttcat tcccttagag ggagctataa
     2461 caaacagatt ttccggatcc ctgggatctt tgaatttttc tttgaaaatc ttaagtgatt
     2521 tgtctccatc gagataagcg cgagcaagtg ctttctccca ctctccagct tccaaagccg
     2581 cttcttgtat ctgcgcttga atatcgggtg ggaaagactt ccatacctgg gaatttacgc
     2641 agaagacaag cggatctata agataattcc aaatggtcat atacttatgg tattcccata
     2701 tcttattgcc aattaaaaca ccctttggat tttcctgacc atcgacaaca ccttgctgga
     2761 aggcagtaac tgcatctccc cagttcatat tgattggatc tgcgccgagc tgcttgaaga
     2821 tatcgatcat aatcggcgat ccgacaacac gaatcttgag gccttcgacg tcctttggtg
     2881 tccttatttc gcgcttggaa tttgttatct ggcggaaacc attttctccc catgcaagga
     2941 acttgacccc gcccctttcc atttcagcaa tcatgcgagc gccagctttg ccattctcga
     3001 tcttgtcgac attatcgtaa gtattgatga agaaaggcag gctgaataga ttgaaaggac
     3061 gaattacagt gcaggcattc atcgtcgatt cggtggcaaa atctatggct ccatccgcaa
     3121 ctgcttgata ccagttggtc tgtttgccag ccagaagcga cgaactccaa tatggcttga
     3181 tatttattac tccgtttgtc ttttgggcaa ccagatcggc gaacttttgt gcgccggcac
     3241 cccaatctac agcttgggtt acatttacct gcattgtata ctctcgtttg ggagtaaatg
     3301 ctgtgactgg caggattgca ataagaagcg ccacgagtaa aagcataaat ctacgataga
     3361 tcatattgac ctcctgatgg caattttcgc tcccaatttt tattctgtca atgttcaatt
     3421 attcaagaat tctgtatatt tagtatcctt cggaatagat gacatattca tgcatagtat
     3481 attgcataaa attctatttc gttttataat atttccaaga aaatgaactt atgcacactg
     3541 tgcatttttg attcggctca aagatagaca atatatggat tagacaccct cagaaaatag
     3601 agctcaaggg ctttatgctc tgttgtgatt tacatattca ttcaatggag aaatggactt
     3661 atgaaaaaga aaatcgtcct tgcttattcc ggtggactgg atactactgt gatagtaccc
     3721 tggcttaaag aacattatga ctgcgaggtg atagcagtct gtggtgatgt tgggcaggaa
     3781 gctgatttcg atgggatcgg tgctcgagcc cttgcttcgg gagcatcgaa gtttatcaag
     3841 ctcgatcaga aagaagagtt tgtgaaggag tacctctggg cactcgtcaa ggcaggtact
     3901 ccttacgaaa aaaaatacct tctcggtacc agcgctgcgc gcccactcct tgccaaaggt
     3961 ctcactgaag tggcgctcgc cgaaggcgca gatgcagtgg cgcatggctg taccggcaaa
     4021 ggcaacgacc aagtgcgctt cgagctcgga gtcaaggcct ttgctccgga gatggaagta
     4081 attgctccct ggcgcatctg ggatattcaa agtagagaag aagagatcgc atacctcgaa
     4141 gcgcgtcata ttcctgttcc gatgaagaaa tcggattcct atagccgcga tgataatctc
     4201 tggcacatca gccatgaagg cctcgacctc gaagatccat cgaacgagcc atactatgaa
     4261 ggcatgctca agatgtgcgt cacaccagaa aaagctcctg acaagccaga atatgtcacg
     4321 cttggattcg agcagggtgt tcctgtatcg atcaatggcg aaaaactcga tccggtatcg
     4381 attgtccgga agctcaacaa aatcggcgga gcaaacggag tcggtatcgt cgatatggtc
     4441 gagaatcgtg tggtgggcat gaaatcgcgc ggtgtgtacg aaacgccagg cggcactctc
     4501 atcatggaag cgcacgatag gcttgaacag ctgtgcctcg acaaaaagac actctcattc
     4561 aaaatgacgg tggcgcagcg ctttgcagag attctctatg aaggggagtg gttcagtcct
     4621 ctttgtaccg cgctgcgagc ttttatagat tcaacccagg atgtcatttc cggacaggtt
     4681 acgctaaagc tctataaagg gaacatggtt tttgctggct cgaaatcttc ctattcgctc
     4741 tatgatgcaa gtcttgcaag ctttactact gggcctttgt tttcacacag cgattcgaca
     4801 ggcttcatca atctcttcgg tttaccgaca aaggtcaggg cgcgactcaa tgagcgaatc
     4861 aagaagtatg gacaagccgc ctctcccgac gtcgtcaaac caggaaattc gggctatact
     4921 gcccatgcgg gagattgata tcttatgggg aaactgtggg aagcaggcga agatggagag
     4981 ctcgatccgg taatagaaca gttcctttcc agcattaaag cggatgtgcg cctaatattt
     5041 gaagacatag aatgttcgca ggcacacgca atcatgcttg gcgagcaggg gataatttca
     5101 ttgcaggatg cgagcttgct cgtagacgaa ttggcaaaaa ttcgcaaaga gc
//
LOCUS       DAOOWY010000122         5141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_16315_length_5141_cov_10.353519, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000122 DAOOWY010000000
VERSION     DAOOWY010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16315_length_5141_cov_10.353519"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /gene="meaB"
                     /locus_tag="PK105_07840"
     CDS             <1..1155
                     /gene="meaB"
                     /locus_tag="PK105_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOU61664.1"
                     /translation="SSLFMNELVGSCIKGDMRSIARLITLLENQDREAYAALYRLKDH
                     EGVAQVVGITGPPGAGKSTLVDKLISQFRAKGRKVGVLAVDPTSPFSGGAILGDRLRM
                     QGHATDAGVFIRSLATRGSLGGISKATHAAIKVLDAAGYDIILVETVGVGQSEIDIVR
                     VADTVVLISVPGLGDDIQVIKAGIMEIGDIFVVNKADRDGADRLVRELKAMLETQAQL
                     NRGQALSGGPDTLAARDPNFMHHAGLVGGLSIGAEKVGFSIGKESGTPAYFDSGQIPI
                     PPVLKTIAATGVGVAELSEAIIQHFKLLKETGELEKKRLESIKYQLGQFIFDEVAQVL
                     NGSPVSEIRDNLAEQVFFRKLDVYSAGMALFNRILSTKEIKGAAHESPKD"
     gene            1136..1540
                     /gene="mce"
                     /locus_tag="PK105_07845"
     CDS             1136..1540
                     /gene="mce"
                     /locus_tag="PK105_07845"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOU61665.1"
                     /translation="MKALKIDHIGIAVEKIDTALSVWSATLGLPLNGIEEVLDQKVKT
                     AFMPIGDTEIELLESTDPEGPIGKFIAARGQGVHHMAFRVENIDKALSELKAKGVRLI
                     DEEPRYGAGGARIAFIHPKATGGVLVELCERT"
     gene            1610..3154
                     /locus_tag="PK105_07850"
     CDS             1610..3154
                     /locus_tag="PK105_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOU61666.1"
                     /translation="MEEKLKELEERRAKVLAGGGPKRVAAQHAKGKMTARERIEMLLD
                     PGTFVELDAFVEHRATELGMDKIDAPGEGVVTGYGMINGRNVCVFAQDFTVIGGSLGE
                     MHAAKICKVMDLAVKIGCPLIGINDSGGARIQEGVDALSGYGEIFYRNTLASGVIPQI
                     SVIMGPCAGGAVYSPALTDFVFMTEGSSNMFITGPQVIKAVTGEDVSPEELGGARIHS
                     EVSGVAHFSLPDEQSTLEAIRKLLSYLPQNNIEDPPLIDIGDDPTRTDDSIADLMPDS
                     PNKPYDVRDIIIRLVDAGDFFEVQPGYAKNIVTGFARLAGRSIGIIANQPKHLAGVLD
                     INASDKAARFIRFCDAFNVPLLTLSDTAGYLPGVEQEHGGVIRHGAKLLYAYSEASVP
                     KITVILRKAYGGAYIAMCSRHLAADQVFAWPTAEIAVMGPEGAANIIFKKEIEESADP
                     EATRKEKIEEYKRSFANPYKAAARGYVDDVIDPAHTRMRLSNALNTLLGKRQSLPSRK
                     HGNIPV"
     gene            3165..3536
                     /locus_tag="PK105_07855"
     CDS             3165..3536
                     /locus_tag="PK105_07855"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOU61667.1"
                     /translation="MTNPWMIMIVGVLTVFISLFIMIFILLAFPSLFAAIKRREHKEL
                     APLPQIVEVSKKQAQPVGGQPVQEDDSLIAVLAAAVAEASGSRLDSFAITQVRQSPAE
                     SVEGFNTPIWGRVERLSRNER"
     gene            3559..4011
                     /locus_tag="PK105_07860"
     CDS             3559..4011
                     /locus_tag="PK105_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOU61668.1"
                     /translation="MKQYRITVNGKSYDVTVEELSGMRQSPSGAFGAGSASYSAPPAN
                     IQPTANVVSSANTAAPTASEQGSSNAAKAAPANNGSILTIKAPMPGTVISFKVIVGQK
                     VKRGDVLLLLEAMKMENEIVAPQDGTVVALRVPASAAVNTGDPLVDLA"
     gene            4029..>5141
                     /locus_tag="PK105_07865"
     CDS             4029..>5141
                     /locus_tag="PK105_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU61669.1"
                     /translation="MEEFLSHFIASTGFIAITWGQIIMLIVSCVLLYLGIAREFEPLL
                     LVPIAFGMLLANLPMSGITDGPRDGLVGGLIYYLYEGVRLGIYPPLIFLGIGAMTDFG
                     PLIANPWSLLLGAAAQLGIFIPFIAAYYLGFDLKSCASIGIIGGADGPTAIYVTSRLK
                     PELLGPVAIAAYSYMALVPLIQPPIMKGLTTKKERQVKMAQLREVSKSERIVFPIFVT
                     ILIGLLLPAAVPLVGSLMLGNLFRETGVTERLSDTAQHALINILTIFLGVSVGSTASA
                     ERFLNPQTLMIIGLGLFAFSCGTAGGVFFGKIMYWVTRGKVNPLIGSAGVSAVPMAAR
                     VSQVVGSKEDPTNFLLMHAMGPNVAGVIGSAIAAGVF"
BASE COUNT         1304 a         1147 c         1371 g         1319 t
ORIGIN      
        1 agttctctat tcatgaatga acttgtgggc agctgcataa aaggcgatat gcgttcgata
       61 gctcgactta ttactctttt ggagaatcag gaccgagaag cctatgctgc cctttatcgc
      121 ctaaaggatc atgaaggtgt cgctcaggtt gtcggaatta caggcccacc tggtgctggc
      181 aaaagtactt tggtagataa attgatttcg caatttagag ctaagggcag gaaggtaggc
      241 gtcctggctg tcgatcctac aagccctttt tccggaggag cgattttagg cgataggctt
      301 aggatgcaag gccatgcaac cgatgcgggt gtttttattc ggagtctcgc tacgaggggt
      361 agcctgggcg gcatttcgaa ggctactcac gctgctatta aggttctcga tgctgcaggc
      421 tatgacatta tccttgtcga gactgttgga gtcggacagt cagagataga catagtaaga
      481 gttgccgata ccgttgttct tatctcagtg ccggggcttg gagatgatat ccaggttatc
      541 aaggcaggta ttatggagat tggcgacatc tttgtagtga ataaggcgga cagagatggg
      601 gcggataggt tagtgcgcga actaaaggcc atgctcgaaa cccaggctca actgaatcga
      661 ggacaagcgc tttctggggg accggataca cttgcggcgc gcgatcctaa ttttatgcat
      721 catgccggtc ttgtgggagg attgagcatt ggcgctgaaa aggtaggctt ctctataggg
      781 aaggagtccg gtacgccagc ttattttgat tccggacaga ttcccattcc tccggtacta
      841 aaaacaattg ctgcaaccgg agttggtgta gctgagcttt ctgaggccat aattcagcac
      901 ttcaagttgc taaaggaaac aggcgagctc gaaaaaaagc gtctcgaatc tataaaatac
      961 cagctaggcc agtttatttt cgatgaagtg gctcaagtgt tgaatggttc tccagtctca
     1021 gaaattcgcg ataatttggc agagcaggta ttctttcgaa aactggatgt ctacagtgca
     1081 ggaatggcac tttttaatag gattctgtcg acaaaagaga taaaaggagc ggcgcatgaa
     1141 agccctaaag attgatcata ttggcattgc ggtcgaaaaa atcgatacag ccctttcggt
     1201 gtggagtgcc acattaggcc ttcctctgaa tggtatcgaa gaagtgttgg accagaaagt
     1261 aaaaactgct tttatgccaa ttggagatac agagatagag cttcttgaat ctaccgatcc
     1321 agaaggtccg atcggcaaat ttattgctgc cagagggcag ggtgtccatc atatggcctt
     1381 ccgcgtcgaa aatatagaca aggctttatc tgagctcaag gcaaaaggcg taaggctaat
     1441 cgacgaggaa ccccgctatg gtgcaggagg agcaagaatt gccttcattc accccaaagc
     1501 gacgggtggt gtgcttgtcg aactttgcga acgaacgtag aattcactct aaattactgt
     1561 cgtagaaagc agcctcctcg ctggaggaat tacaacaagg atggtcggta tggaagaaaa
     1621 gttaaaggaa ctagaggagc gccgagcgaa agtgcttgcc ggtggtggcc ctaagagggt
     1681 tgcggcgcag catgcaaaag gcaagatgac tgctcgcgag aggatcgaaa tgctgctaga
     1741 tccaggcacc ttcgtggaac tcgatgcctt tgtcgaacac cgagcgaccg aacttggtat
     1801 ggacaagatc gatgctccag gtgaaggagt cgttacaggc tatggaatga tcaatggaag
     1861 aaatgtatgt gtatttgccc aggatttcac ggtgataggt ggaagccttg gtgagatgca
     1921 tgccgctaaa atatgcaagg tcatggatct cgcagtaaaa atcggttgtc cattgatagg
     1981 aataaatgac tcaggtggcg ccagaataca agagggcgta gatgctctat cgggctatgg
     2041 tgagattttt tatcggaaca cgcttgcatc tggagtcatt cctcagattt cggttattat
     2101 ggggccttgt gccggagggg ctgtttattc gcccgctttg acggatttcg ttttcatgac
     2161 agagggatcg agcaatatgt tcataacagg ccctcaggta attaaagcag taactggtga
     2221 agacgtaagt cctgaagaac taggaggcgc tagaatacac tcagaagtct cgggtgtggc
     2281 gcatttcagc cttccggatg aacagtcgac tttggaggcg ataagaaagc tgctgtccta
     2341 tctgcctcaa aacaacatag aagatccccc cttgatcgat atcggagacg atccgactcg
     2401 gacagatgat tcaatcgcag acctaatgcc ggattctcct aacaagccct atgatgtccg
     2461 agacattatc attcgacttg tggacgctgg ggactttttt gaggttcagc ctggatacgc
     2521 aaagaacatt gtgacaggct ttgcgcgcct agccggccga tccataggaa taatagcaaa
     2581 tcagcccaag catctagcgg gtgtgctcga tatcaacgcg tccgataagg ccgcacgctt
     2641 catccggttc tgcgacgcat tcaatgtacc gcttctcact ctttcagata ccgctggtta
     2701 tcttccgggc gtggagcagg agcatggcgg tgtcatcagg cacggagcaa agctcctcta
     2761 tgcttactcg gaagcatctg tgcctaagat aaccgtgatt ctgcgcaaag catatggcgg
     2821 agcctatatc gctatgtgtt cccgtcatct cgctgccgat caagtctttg cctggccaac
     2881 tgcggaaatt gcggtaatgg gtcccgaggg tgctgcgaat atcatcttta agaaagagat
     2941 agaagagtcc gctgatccgg aggcaacccg caaggagaag atagaggaat ataaacgcag
     3001 ctttgcaaac ccttataaag cagcagcgcg aggctatgtc gatgatgtca tcgatccagc
     3061 tcatacaagg atgcgtctaa gcaatgcatt gaatacgctg cttggaaaga ggcagagtct
     3121 gccttcacgc aaacacggga atattcctgt ttgaggggtc tagcatgaca aatccatgga
     3181 tgataatgat tgtcggagtc ctgacggttt ttatatcgct attcatcatg atttttattt
     3241 tgctggcttt tcccagtctc tttgcagcaa taaagcgtag agaacataag gaactggcac
     3301 ccttgcccca gatcgtcgaa gtttcaaaga aacaggctca accagttggc ggccaaccag
     3361 tgcaagaaga cgattcattg attgccgttc ttgcggcggc tgtagccgaa gcgagtggtt
     3421 cgagacttga tagttttgca attacgcagg tgcgtcagtc accggcggag agcgtagaag
     3481 gcttcaatac cccgatatgg ggcagagtag agcgcctttc ccgcaatgaa cgctagaaca
     3541 tttgaggaga aatatattat gaaacagtat agaattacag taaatggcaa atcttatgat
     3601 gtgacagtcg aggagctcag tggaatgcgg cagtctccgt caggtgcatt tggtgcaggc
     3661 tctgcttctt attccgcacc accagcgaat attcagccta cggcaaatgt agtctcatcc
     3721 gcaaacaccg cagctcctac cgcctctgaa cagggttctt ccaatgcagc caaggcagcg
     3781 ccggcgaaca atggctcaat tctaacgata aaagccccga tgcctggaac cgttatttcg
     3841 ttcaaagtaa ttgttggaca aaaagtaaag cgcggcgatg ttcttctcct attagaggcc
     3901 atgaaaatgg agaatgaaat tgttgctcca caggatggta ctgtcgttgc tctccgcgtt
     3961 cccgcaagcg ccgcagtaaa tacaggcgat ccgctcgtcg atctagcata gaccaggagg
     4021 attcctcaat ggaagagttc ttgtctcatt ttattgcttc gacaggtttt atcgcaatta
     4081 catgggggca gatcataatg ctcattgtat cgtgcgtgct tctatatctt ggcattgcgc
     4141 gagaattcga gcctcttctc ctcgtgccta ttgcatttgg catgcttctg gctaatcttc
     4201 cgatgagcgg cattaccgat gggccaaggg acggtctagt tggtggcctt atctattatt
     4261 tgtacgaagg cgtaaggctt gggatatatc cgccgctaat ttttcttggc ataggcgcaa
     4321 tgaccgattt tgggcctcta atcgcaaatc cctggagcct tcttctaggt gcagcggctc
     4381 aattaggcat tttcataccc tttattgcgg cttactattt gggattcgat cttaagtcct
     4441 gcgctagtat tggaatcatc ggaggtgcag atggccctac cgcaatctat gttacttcgc
     4501 gtttgaagcc cgagcttttg ggtcctgttg ctatcgcggc atactcgtac atggcgcttg
     4561 tgccgctgat tcagcctcct atcatgaaag ggctcacgac gaaaaaagag cggcaagtaa
     4621 agatggccca gcttcgagaa gtctccaaat cagagcggat agtgtttcca atatttgtca
     4681 ctattctaat agggcttttg cttcctgccg cagtccctct cgtaggttcg ttgatgcttg
     4741 gcaacctatt cagggagaca ggcgtgacag aaaggctctc cgataccgcg cagcatgcgc
     4801 tcatcaatat tttaacaatt ttcctaggag tgtcggttgg ctcgaccgca agtgcagagc
     4861 gttttctcaa cccccagaca ttgatgataa taggactcgg actttttgct ttttcctgtg
     4921 gaacagcggg aggagtattc tttggaaaga ttatgtactg ggtcacccgt ggaaaggtga
     4981 atcctcttat aggatccgca ggcgtctcgg ccgtgccgat ggctgcccgt gttagccagg
     5041 tggtgggttc taaagaagat ccaaccaatt tcctgctcat gcacgctatg ggtccgaatg
     5101 ttgcaggagt catcggttcc gcgattgccg ctggagtctt t
//
LOCUS       DAOOWY010000123         5118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_16439_length_5118_cov_9.628876, whole genome shotgun sequence.
ACCESSION   DAOOWY010000123 DAOOWY010000000
VERSION     DAOOWY010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16439_length_5118_cov_9.628876"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..2720
                     /locus_tag="PK105_07870"
     CDS             408..2720
                     /locus_tag="PK105_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61670.1"
                     /translation="MNFYHSYRGDTPDERGFGKDIRVIRYILDSLDELSKDGIEVKCA
                     WDFDNAFTLGHILPSFAPDIIERIKMRVIAGVDEIELMSWNNGLLTAHTPEELRYALS
                     WAINAPDGSGVLQTFGSYVPIARPQECMFTGSHIDIYRSVGIEAISLYYSAIPFNAIG
                     SFISELEARKRYNPLTLKNPTTGASIRLIPAINQGDLAEYYFSAARMLKAIRAEQLHE
                     TFRCAHGLDAEGDPERDIEMGIEGDSGGIAQPVDMLVVLDIDADDSFWAGFLPGFSRR
                     FVPSFGGLYSLIASIARLPFVGFERPWEYIKTHPDRGEIVIGQDMADGAFDGYSSWSE
                     KYENYEIWSIIAEARSQWQSAKSIVAVRNGESVLRSATSPKTETYWLDELSELDPSLA
                     RLAHEAIKERLRALSTTHFGLAVPVMNVTRLEIARETANRSLELAKAFYTAVKHKYGV
                     VNAISNLPCIKEPQELRGPKLPQAAHPESRVRIQDSSPHSPHLTLSVSSKGRIRLASG
                     PYTVHINEPWVEYNHKILGSRKIDVISGAERYELHGIIPLRPMRVAIEWSRTATMLSA
                     DLVCFELHVQYPSTPHRCYDHKKARTLGRTWDDRWRQLAPFEIVAFENLPCSTIIHVW
                     KEDFSNVISHYSLDYAGYGRNRFLADINNHLTPKWLAVSDGARGILVAQASQSFSSYA
                     FCPLRQDLRCGVQCVSMFPFGALWGPQYRYPAAVTGLGRRAAILTAEHLHSSAPSWEG
                     KTLDARLLIALYEGNEPQRSLLAEVHECLL"
     gene            2998..4545
                     /locus_tag="PK105_07875"
     CDS             2998..4545
                     /locus_tag="PK105_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU61671.1"
                     /translation="MTKKEHSKPLAKQVPSPEGGPALTSISRASLTTERPVTFKTLIA
                     YGVGDIYGGGAFIIVSLFYMFFMTEVVGLSPALAGLALGIGKVWDAITDPLMGYLSDH
                     TRSRYGRRRVYFLVGIVPIALSYIIMWIRVQASSQVALFFYYLFAYILFDAVITMVMT
                     PYSALAAELSADYKVRNRLSAARLFFSGFSSLLASTVPSMIINAHKEDPAQGYLIMSI
                     VFGILFAIPWIFVFWGTWENPHLASPTVSRDFMKEFASILKNKSFRIHLMMYVFAYSA
                     MDMLMGLFTYYLTYYLQRPNIYSIAMGTLMIVQLFMILVYTKVANTKGKRFAYILGMS
                     IFLAGLYATIVLTSSTPLLVLVLICAVIGLGTAAAVYIPYAILPNVIDVDELMTGQQR
                     AGVYSGAMTLARKVVQGIIVMPLIGFFLQLVGFQSNQAQTPEVLSKFLIFFITGPSIF
                     LILGIIVAMRFELSPKNSEILVKELERIRAGGRLEDVSPEVKTICEKLTGMRYEECWN
                     KKGATEI"
     gene            4926..>5118
                     /locus_tag="PK105_07880"
     CDS             4926..>5118
                     /locus_tag="PK105_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61672.1"
                     /translation="MKEDIEQKEKAQGGTPERLKVGGMFAYGAASVGMGLFYAFNNFS
                     LPLFYRTYTSSDALIGLLAN"
BASE COUNT         1336 a         1141 c         1226 g         1415 t
ORIGIN      
        1 gaatcgattt cttgactgtt gctcaactgt gactcgattg gtttgcttcc agatgaacca
       61 cttgactgtg gggcttgtgg ggctatccaa agaggccgtt ataaggctca ttaccatgag
      121 cacattggta aaaaagtgta gcacctgcgt tcctgcgaaa ttatacaaaa gatatcatta
      181 acttatagct ttattagaga ataatgcagg gcatctttct ctcaagagga cagtcagagc
      241 gaatagcggt agtgaaaaaa agcttgaaac gctatattga aagaaggccg gaaagaggca
      301 tatataggac acagaaagat aagagcttca aaaccggcta atggagattt tgctaaagaa
      361 aaatggtagg cagcaacaca aaaatctttt tttgtcctcg gtttcacgtg aatttctatc
      421 attcttatcg gggagatacg cctgatgagc gcgggtttgg caaggacatt cgcgttattc
      481 gctacattct tgatagcctc gatgagcttt ccaaagatgg aatcgaggtc aagtgcgcat
      541 gggatttcga taacgcgttc accttgggac atattctgcc gagctttgct ccagacataa
      601 tagagcggat taagatgagg gttattgctg gggtcgacga aatcgaactt atgtcctgga
      661 acaatgggct tcttaccgct cacacacctg aggagctaag gtacgctctt tcatgggcaa
      721 ttaatgcgcc cgatggttca ggagtgcttc agacctttgg ttcatatgtg ccgattgcac
      781 ggccgcagga atgtatgttt acgggatcgc acatcgacat ctatcgtagt gtggggatcg
      841 aagctatatc tttatattac agcgcaattc ctttcaatgc gataggttcg tttatttctg
      901 aattggaggc aagaaagcgg tacaatccac tcacgctcaa aaaccctaca acaggagcat
      961 cgataaggct tattcctgcg atcaatcagg gcgatttggc tgagtattat ttcagcgccg
     1021 cccgcatgct caaagcaatt cgagctgagc agttgcatga aacttttcgt tgtgcgcacg
     1081 gcctagatgc tgagggcgat cctgagagag atatcgagat gggcattgaa ggagattctg
     1141 gaggcatagc acagcctgtc gatatgctag tggtgctcga tatcgatgca gacgatagtt
     1201 tttgggcagg ctttttgcca ggtttttctc gtcgctttgt gccatcattt ggagggcttt
     1261 attcgcttat cgcgagcatc gcgcgcttgc catttgttgg ttttgaacga ccatgggaat
     1321 acatcaagac ccatcctgat aggggagaga ttgttatagg ccaggatatg gctgatggtg
     1381 cctttgatgg ttattcatcc tggtctgaaa aatatgagaa ttatgaaatt tggtcgatta
     1441 ttgccgaagc gagatcgcaa tggcagtctg caaaaagcat tgtggcagtt cgaaatggtg
     1501 aatctgtatt gcgttctgca actagcccaa aaaccgaaac atattggctc gatgaactct
     1561 cggagctcga tccatccttg gccaggcttg cgcatgaagc gataaaagag cgacttcggg
     1621 cgctgtctac aacccatttt gggcttgctg ttcctgtaat gaatgtgaca aggcttgaga
     1681 tagctcgaga aacagcgaat cgatccctcg agctcgctaa ggccttctat acggcagtaa
     1741 agcataaata tggtgtagtc aacgcgatat ctaatctacc ctgtattaaa gaacctcaag
     1801 aactccgggg tccgaagttg cctcaagcag cgcatccaga aagcagggtt cgcatacaag
     1861 attcttctcc tcacagtcct catctcacgc tttcggtcag ctcaaagggc agaatccggc
     1921 tagccagcgg accatataca gttcatatca acgagccctg ggttgaatat aatcataaaa
     1981 ttctcggttc gcgcaaaatt gacgttatat ccggtgctga gcgatatgaa ttgcatggaa
     2041 tcatccctct aaggcccatg cgagtggcga tcgaatggag caggactgct actatgctca
     2101 gcgcggatct tgtgtgcttc gagcttcatg tgcaatatcc atcgaccccg caccgatgtt
     2161 atgatcacaa aaaggcaaga acactcggtc gaacatggga cgatcgctgg cgccagcttg
     2221 cgccttttga gattgtcgca tttgaaaatc tgccctgttc taccattatc catgtatgga
     2281 aagaggactt tagtaatgtc atttcgcact attcgctaga ttatgctggc tatggtcgaa
     2341 atcggttcct tgcagatatc aataaccacc ttacgccaaa atggcttgca gtgagcgatg
     2401 gcgcgcgagg gattctagtt gcgcaggctt ctcaatcttt tagcagttat gctttctgtc
     2461 ctctacgcca ggatttacgg tgtggtgtac aatgcgtgag catgttccct tttggtgctc
     2521 tttggggacc acagtaccga tatcctgctg cggtaactgg cttaggaagg cgcgctgcca
     2581 ttctgactgc ggaacatctg cattcctcgg ccccctcatg ggaaggaaag acccttgatg
     2641 cgcgccttct tatcgcactc tatgagggta atgaaccgca gagaagtctt ctagcagaag
     2701 tgcacgaatg cttgctctga taaaggcgaa aatcaaagcg tcatgagatg tatctattct
     2761 ctttcattcc aaagaattca ttccaaaaaa gtcggaagca aaaatgtacg tttgctaatg
     2821 cctgatatca atacaatatt gcagacaaat gagcacggaa gaaaggaaac ccattctttg
     2881 cgatgcatag atgacagcac aagtaagatc cggtaaaatc gatttgacca atctcagtgc
     2941 acataatttg ggaacatgag gcgtaagata ctgccggcaa aagcaaggag gcagagtatg
     3001 acaaaaaaag aacattcgaa gcctctcgcc aagcaagtgc catctccgga gggtggacct
     3061 gctctgactt ctatttcaag agcttcgctc actaccgaaa gacccgttac ctttaagacg
     3121 cttatcgcct atggagtcgg cgatatatat ggaggtggcg cattcataat cgtaagcctt
     3181 ttttatatgt tcttcatgac cgaagtcgta ggcctttctc ctgcccttgc aggccttgcg
     3241 cttggtattg ggaaggtttg ggatgctatc accgatcctc ttatgggcta cctttcagat
     3301 catacacgtt cgcgttatgg ccgcaggcgc gtttatttcc ttgtgggtat tgtgcccatc
     3361 gcgctttctt acataatcat gtggattcgc gttcaagcaa gctcgcaagt cgcgcttttc
     3421 ttttattatc tcttcgccta tatcctcttc gatgcggtga tcacgatggt gatgaccccc
     3481 tattctgcgc tcgctgctga gctatccgct gattataagg tgcgtaatcg cctctctgct
     3541 gcgaggctct ttttctcagg cttttcatcg ctgctggcat cgaccgtgcc gagcatgatc
     3601 atcaatgcac ataaggaaga tcctgcgcaa ggctatctta tcatgtcgat tgtgtttgga
     3661 attctatttg caattccctg gatatttgtt ttttggggaa catgggagaa tccacatctt
     3721 gcaagcccaa ccgtatcgcg agattttatg aaggaatttg cgagcattct taaaaacaaa
     3781 tcctttcgga ttcatcttat gatgtatgtt ttcgcctatt ctgcaatgga tatgttgatg
     3841 gggctcttta cgtattacct tacctattat ttgcaaaggc cgaatattta ttcaatcgca
     3901 atggggacgc tcatgatcgt gcagcttttt atgattctcg tctataccaa ggttgcgaac
     3961 accaagggca agcgctttgc gtatatcctt ggcatgtcga tctttcttgc tggtctctat
     4021 gcgactattg ttcttacatc gtctacgcca ttgctagttc tcgtacttat ctgtgcggtc
     4081 atagggcttg ggactgccgc tgcggtctat attccatatg ccattttgcc caatgtaatc
     4141 gatgtagatg agctaatgac aggtcaacag cgcgctggag tctattctgg cgcaatgact
     4201 cttgcgcgaa aggttgttca aggtatcatc gttatgccgc tcatcggatt cttccttcag
     4261 ctcgttggct ttcagtccaa ccaggcgcag acgccggaag tactcagtaa attcttgata
     4321 ttcttcatca ccgggcctag tattttcctt attttgggca tcatcgtcgc aatgcgcttt
     4381 gagctcagcc caaaaaacag cgagatccta gtcaaagaac ttgaacgaat ccgcgctgga
     4441 gggaggttgg aagatgtcag cccagaagtc aaaaccatct gcgagaagct aaccggcatg
     4501 cgctatgagg agtgttggaa caaaaaaggc gcaactgaga tatgacaata tttcagaagc
     4561 atcctcagga attcgtgcaa acatttgaat tgatagcaat ttcatctgca aatatcgtat
     4621 caacaagtag atttgcatgc tggcttctat attctatatt cctataataa cttatagtat
     4681 ctagagaaaa ggaagagcat gcaaaagtcg gcgaaaaaac ctatcatgga cgaattgctg
     4741 tccaaactaa tgcggcatag cagattcgtt caagccaccc acatcgaatc agattataat
     4801 tcctttgcat aattagtagc tctcgcgata tctcgagcaa aatggatttg acgctggcca
     4861 catcgcgcaa taaactcaag atagaatgag ctaagtggca cgggagataa tatgaggagg
     4921 tagatatgaa ggaggatatt gagcaaaaag aaaaagctca aggagggacg cctgaacgcc
     4981 ttaaagtcgg gggtatgttc gcctatggag cggccagcgt gggtatgggc ttattctatg
     5041 ccttcaacaa tttcagcctt cccctctttt atagaaccta cacttcgagc gacgctctca
     5101 ttggccttct tgcgaaca
//
LOCUS       DAOOWY010000124         5073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_16634_length_5073_cov_9.624552, whole genome shotgun sequence.
ACCESSION   DAOOWY010000124 DAOOWY010000000
VERSION     DAOOWY010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16634_length_5073_cov_9.624552"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PK105_07885"
     CDS             <1..418
                     /locus_tag="PK105_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOU61673.1"
                     /translation="DSQEKLLQLLEDHGYSVTQATLSRDLKYLKVGKLSDGHSGYYYS
                     IPSEEERRESERHYIQDFLRGYVSLDWNQSLVVVRTFSGHSDSVALAIDNMGFDEVIG
                     TISGRDNVVFIALKKGVSGDDFIRSLKAKIPEFEEE"
     gene            568..3153
                     /gene="clpB"
                     /locus_tag="PK105_07890"
     CDS             568..3153
                     /gene="clpB"
                     /locus_tag="PK105_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOU61674.1"
                     /translation="MNYEQFTLKAQEALRNAASIAQKNDHSSIEPEHLLLALLDQEGG
                     VVSSIIERLGVDRTKLEQRLADLLKTLPKAYGETAQVYFSPAMQKVIAKAESESESMK
                     DEFVASEHLFLALLITESRAASLLRSIGVTHEGVLAALKSIRGSQTASDENAEEKYQV
                     LEKYTRDLTALARAGKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIISGDVPDSLKDKKVLALDLGAIVAGSKFRGEFEERLKAVITEITKSEGKIILF
                     IDELHTLVGAGAAEGAMDASNLLKPALARGELRAIGATTLDEYRKHIEKDAALERRFQ
                     PIYCAPPSVEDTIAILRGLKERYEVHHGVRIRDDALIAAAVLSDRYITSRFLPDKAID
                     LVDEAASRLKMEIESQPTELDQIQRRLIQLRMEADALKKETDPASIERRSKLEKEIGE
                     LSEKRDEMMARWQQEKQLIEDIRQLKQQIEEYKIQVDQLVREGQLAKASEIKYGKLVE
                     AQQKLEELSKKVQEEEDKPRLLREEVSDEDIAKVVSLWTGIPVSKMLSSEIQKYVELE
                     SQLGKRVIGQEKALRAVADAIRRNRAGLSDPRRPMGSFLFVGPTGVGKTELAKALAEF
                     LFNDERALTRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLFDEI
                     EKAHPEVFNVLLQLLDDGRLTDGQGRVVDFRNAIIIMTSNIGSEFILTMKSMDEIRDR
                     IDGLLKTYFKPEFLNRIDEIVVFDRLDESMIRNIAKLRLSELADRLAQRNIKIEIQDK
                     AIDYIAKEGYDEQFGARPLKRAIQNLLENPIAKKVLSGEIMEGDTIHVSLSGSELAIS
                     TEHAA"
     gene            complement(3177..4067)
                     /locus_tag="PK105_07895"
     CDS             complement(3177..4067)
                     /locus_tag="PK105_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61675.1"
                     /translation="MAQAVFDEAFMLLYPDLSQAVASKFQLDSVASPGAATQLLEKMT
                     DPKTTDNQSLKIIIASPSVTPVIQEFASKGCFALINLLTPANGFYSVEWDNAWAYRQI
                     GLLAGYRLAVLQKETDKNASAALLFARGIGRSDTELEVFNRAFRESYLLATMGTEQEN
                     SSNQAAIMPENALKVFDVDNLNLPGDRLEQVLSVVEQIEEIKPGVLIFACTSRLALEK
                     TCEIQGIDIIADIRGLGKDIPHKKLFASLGENRSNLLRALLKTVKEIEDGNTEPHSVY
                     LKPSLELSKEARRIQQELKP"
     gene            4270..>5073
                     /locus_tag="PK105_07900"
     CDS             4270..>5073
                     /locus_tag="PK105_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOU61676.1"
                     /translation="MISYKELGLVNSVELFRKAMEGGYAIPAYNFNNMEQMQAIIQAC
                     VETKSPVILQVSSGARKYANATLLRSMAKGAVEYAKELGYPIPIVLHLDHGDSFELCK
                     DCIENGFSSVMIDGSHLPYDENVALTKRVVEFAHAQKDYVSVEGELGVLAGIEDEVSA
                     EKSHYTRPEEVEDFVKKTGVDSLAISIGTSHGRAKFKPEQCTRTSDGVLIPPPLRFDI
                     LEEVERRIPGFPIVLHGSSSVPVEYVRMIEQYGGKLPDSVGIPEDQLRKA"
BASE COUNT         1428 a         1105 c         1257 g         1283 t
ORIGIN      
        1 cgattcgcag gaaaagctgc ttcagctttt ggaagatcat ggctattcgg tgacgcaagc
       61 gacactttct cgcgatttga agtatttaaa ggtcgggaag ctctccgatg ggcattctgg
      121 ctattactat tccattcctt cagaagaaga acgcagagaa tcagagcgtc actatatcca
      181 ggattttttg agaggctatg tttcgctcga ttggaatcag agcctcgtgg ttgtgcgtac
      241 cttttcaggt cattcggatt cggttgccct tgcaatcgat aatatgggct ttgatgaagt
      301 gataggcacg atatcgggac gcgataatgt tgtattcatc gcgctcaaga agggcgtgag
      361 cggcgatgat tttattcgct cactcaaggc aaagattccc gaattcgagg aagaataatc
      421 ggcgcttata tattgcacaa tcattcggtg attcatagtc aactgagcat gggagagcaa
      481 tttttataat ttaatttgcc aaaagcctgt ctgaatttgt atacttaata gagaagaatc
      541 tcttttgaat cacgaaggaa atacactatg aattatgaac aattcacctt aaaagcgcag
      601 gaagcgctcc gcaacgcagc ttccatagct cagaagaatg atcacagctc tatagaacct
      661 gaacatctgc tcctggcctt attagatcag gagggaggag tcgtatcttc gatcatagag
      721 cgcttaggag tagataggac caagcttgag cagaggctcg ctgatctgct taaaacgctg
      781 ccaaaggcat atggcgaaac ggctcaggta tacttttctc ctgccatgca gaaggtcatt
      841 gccaaagccg aatccgaatc cgaatcaatg aaggatgaat tcgtagccag tgagcacctt
      901 ttcttagctc ttttaataac ggaatccaga gcagcctctt tgcttagatc gataggagtc
      961 acgcacgaag gggtgcttgc agccttaaag tcgataaggg gttctcagac cgcttccgat
     1021 gagaatgctg aggagaaata tcaggtctta gaaaaataca cacgcgatct caccgccctt
     1081 gctcgtgctg gcaagcttga tccggtaatc ggtagagatg aagaaattcg cagggtaatg
     1141 caggtccttt ccagaagaac aaagaacaac cccgtgctta ttggagagcc cggagtaggg
     1201 aaaacagcca ttgtggaagg cttggcgcag cgtataatat cgggcgatgt cccggacagc
     1261 ctaaaggaca aaaaggttct cgccctcgat ttaggagcca tagtggccgg aagcaaattt
     1321 cggggcgaat tcgaggaacg gctcaaggcc gtcatcaccg agatcaccaa atctgaaggc
     1381 aagataatac tcttcataga tgagcttcat actctggttg gagccggagc cgcggaagga
     1441 gctatggatg cgtcaaacct cttaaagcca gcccttgcgc gtggggagct tcgcgccata
     1501 ggtgcgacta ctctcgatga gtatcgcaag catatcgaaa aggacgctgc gcttgagcgt
     1561 agatttcagc ctatttattg tgcaccccct agtgtggagg acacaatagc tatattgcga
     1621 ggccttaagg agcgttatga ggtgcaccat ggagtgcgaa ttcgcgatga tgcgcttata
     1681 gccgccgcag ttttatccga tcgctatatc acctctcgat tcctgcctga taaggctata
     1741 gatctagtcg acgaagcagc ctcaagactc aagatggaga tcgagagcca gcctacagaa
     1801 ctagatcaga ttcagcgcag gttgattcaa cttcggatgg aagcagatgc tcttaaaaaa
     1861 gagacagacc ctgcttccat agagagacgg agcaagctcg aaaaagagat aggcgagctc
     1921 tccgaaaaac gcgatgaaat gatggctcgt tggcagcaag aaaagcaatt gattgaggat
     1981 attcgtcaac ttaagcaaca gatcgaggaa tataaaatcc aagtagatca gcttgtgcgc
     2041 gaaggtcaac ttgccaaggc ttctgagatc aaatatggca aactagttga agcgcagcaa
     2101 aagctcgaag aattatcgaa aaaagtccaa gaggaagagg acaagccaag acttctgcgc
     2161 gaagaagtct ccgacgaaga tatagccaaa gtcgtgtctc tctggacggg cattcctgtt
     2221 tccaagatgc tttcgagcga aatacaaaaa tatgtcgagt tagagagcca gttaggaaag
     2281 agggttatcg gtcaagaaaa ggcgctgcga gccgtagccg atgcaatacg ccgtaacaga
     2341 gcggggcttt ctgatccgcg ccgacctatg ggctctttcc tctttgttgg accgacggga
     2401 gttggaaaga cagagcttgc aaaggcgctc gctgaatttc tcttcaatga tgaacgagct
     2461 cttacgcgga tcgatatgag tgaatacatg gaaaagcact cagtgtcgag gctcatcggg
     2521 gctcctcccg gatatgttgg atatgaggaa ggtggtcaac tcaccgaggc ggtgagaagg
     2581 cgcccctatt ctgtagtact attcgatgag atcgaaaagg cccaccccga agtattcaat
     2641 gttcttctac agcttctgga cgatggacgg ctaacagatg gacagggaag ggtcgtagat
     2701 tttcgtaatg ctattatcat catgacaagc aacataggct cggagttcat tctcaccatg
     2761 aaaagcatgg atgagattcg agatcggatc gatggacttc ttaagacata cttcaagccc
     2821 gaattcctaa atcgcatcga tgagatcgtt gttttcgatc gcctcgatga atccatgatc
     2881 cgcaatattg ctaaattgag gctcagtgaa ctcgcagatc ggcttgcgca gagaaatatc
     2941 aagatcgaga tacaagacaa ggccattgat tatatcgcaa aggaaggata cgacgaacaa
     3001 tttggcgcgc gtcctcttaa aagagcaata caaaacttgc ttgaaaaccc cattgccaag
     3061 aaagtccttt ccggggaaat catggaaggc gataccattc atgttagcct ttccggtagc
     3121 gagcttgcca tcagcaccga gcatgccgca tgagaaagca ccttatcgca gagtgcctaa
     3181 ggttttaact cttgctggat gcgccgagcc tctttcgata attcgagact aggttttaaa
     3241 tatacggaat gaggctcggt gtttccatct tctatttctt tcactgtctt taggagcgct
     3301 ctcaggagat tggatctatt ctctccaagc gatgcaaata atttcttatg cggaatatct
     3361 ttccccaggc ctcttatatc ggcaatgata tctattcctt gaatctcaca agttttttcc
     3421 agcgcaagcc tgctagtgca agcaaagatc aaaacgccgg gttttatttc ttctatttgt
     3481 tctaccacag agagcacttg ctccagccta tctccaggaa gattcagatt gtctacatcg
     3541 aatactttaa gagcattttc gggcataatt gcggcttgat ttgaagaatt ttcttgctca
     3601 gtgcccatgg tagccagtag ataactttct ctaaaggctc gattgaatac ttccagctct
     3661 gtatcgctcc ttcctatacc gcgagcaaag agcagggccg ctgaggcatt tttgtctgtc
     3721 tctttttgga gaacagcaag gcgatagccc gctaagagcc caatctgtcg gtatgcccat
     3781 gcattatccc attcgacaga ataaaagcca ttggctggag ttagaaggtt tattaacgca
     3841 aagcagcctt tggaggcgaa ttcttgaatt actggagtta cagatggact ggctatgatg
     3901 attttcaaag actgattatc cgttgttttc ggatcagtca ttttttccaa aagctgtgta
     3961 gcggccccgg ggcttgccac actatccagc tgaaatttac ttgcaacggc ttgggagaga
     4021 tctggataga gaagcataaa ggcttcatca aagaccgcct gtgccatcgg atgtctgcag
     4081 cttgagaata ggaggctgat caggacaatg gtagtataca gcattttgcg agcgggtccc
     4141 tgacggaaag aaatgcagaa gtgcttattt ttgtaccaca tttgataagc ctgttgcata
     4201 atcgcataat tctctatata acgacaataa cctgcagcaa agagaattta taatttttcg
     4261 gaggattata tgatcagtta taaagagctt ggcctggtaa acagtgttga acttttccgc
     4321 aaggctatgg agggtggata cgctatcccg gcttataatt ttaacaacat ggagcagatg
     4381 caagccatca ttcaagcttg cgttgagacg aaaagtcctg tcatacttca ggtatcctct
     4441 ggtgcacgga aatatgcaaa tgccacgcta ttacgatcga tggcaaaagg cgccgtcgaa
     4501 tatgcaaaag agctaggcta tcctattccg attgtattgc acttagatca tggagatagt
     4561 ttcgagttat gcaaggactg catagaaaac ggcttttctt ccgtaatgat agatggttca
     4621 catctccctt atgacgaaaa tgtggctctt acaaaaagag tggttgaatt tgcgcatgct
     4681 cagaaggact atgtcagtgt agaaggagag cttggggtgc ttgcaggtat agaagatgaa
     4741 gtatcggcag aaaaaagcca ttacacccgg cctgaagaag tcgaagactt cgtcaaaaag
     4801 acaggagtcg atagtctggc aatctctatc ggcacaagcc acggtcgcgc gaagttcaag
     4861 ccagagcagt gtactcgcac ttctgatgga gtgctgattc ctcctccgct tcgcttcgac
     4921 atcctcgaag aagtcgaaag gcgaattcct ggattcccaa ttgttcttca tggttcttcc
     4981 tctgttcccg tagagtatgt gcgcatgatc gagcaatatg gtgggaagct tccagactct
     5041 gtgggcattc cagaagatca gcttagaaaa gca
//
LOCUS       DAOOWY010000125         4985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_16995_length_4985_cov_12.491684, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000125 DAOOWY010000000
VERSION     DAOOWY010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16995_length_4985_cov_12.491684"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK105_07905"
     CDS             complement(<1..424)
                     /locus_tag="PK105_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOU61677.1"
                     /translation="MISFIVILSIGLAIAAIVEYKDHCARLKAVPIRILVNGTRGKST
                     VTRLIAAGLAAAGLRVYAKTTGSAARIILPDLSEREIRKHGTAKRRPSIMEHKWFIRE
                     AHRAGAQAIVAECMAIRPETIGVLETRLAHSTIGVITNV"
     gene            complement(497..982)
                     /locus_tag="PK105_07910"
     CDS             complement(497..982)
                     /locus_tag="PK105_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61678.1"
                     /translation="MNTVYLSKDLAMERVSSFLSRWKNSTDQDIDFDLAKSSEIIEDA
                     SELLRKVPREVQKIGCYCLASQRKVVVWLLTDESGHGSGDEERGQNFLQIVLQKPPEF
                     GSVPVEIKYNVMSNSADRNNNVCLCGIEISYGNILILSPPATGEMVISISRIELAKEL
                     S"
     gene            complement(1026..2120)
                     /locus_tag="PK105_07915"
     CDS             complement(1026..2120)
                     /locus_tag="PK105_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOU61679.1"
                     /translation="MRKFFLAWFVLLGLFVSQAQTEDGVAFVMSEVKAGKGVEKVLSL
                     SAFLSSLEGTAFDTPVFFIGDSPVLHVKNAPERSISEETTEKQDSSSTDYTVAIVAGA
                     HANEIAGSLAAYWIIEHCRVSGGKLIVVPRANASAATWSTSKAAAPILHYGARLSNPA
                     HEKSPDPGFFIPFDASESFPSLSGQEVRNLNRQYPGDPTGNYTSQIAYAITRLLVQNH
                     VRTALDLHEASPGSSLAWSIITRPEYLDVAALAVLQIEEETEKSFHLESSRDEFAGYS
                     HWEWGKLGIRAFLVETLNPAQPSDDPTIDQIFNDKAPLAERVFIHLCAIEALVASAYE
                     EIGDSVALNIEGLPSSKEEVQKWLLGIEKN"
     gene            complement(2117..2710)
                     /locus_tag="PK105_07920"
     CDS             complement(2117..2710)
                     /locus_tag="PK105_07920"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOU61680.1"
                     /translation="MIYRYSSTKRFLAIVCFMLLGILPIFAQYSVPSTLVASFERPGF
                     ITSAGQTSDAAIVKVLANTRLKLGLEYDIAAKPETIANAKTLVLVLGVSNKGLGAAGL
                     SIEQEIERVQSIVAEALKNNTRIISIHTGGTARRGEASNQVIELCVPASDVVIVVEGG
                     NNDGFFTSLCEENNVFLVQVPSIAEAGNVLKSLFEAQ"
     gene            complement(2722..4392)
                     /gene="ggt"
                     /locus_tag="PK105_07925"
     CDS             complement(2722..4392)
                     /gene="ggt"
                     /locus_tag="PK105_07925"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOU61681.1"
                     /translation="MAQKNKSVGLGMKHGVRKFLLFLLAAFVFALPIFAADTINAEIT
                     ARNGMVASAQPLASAAGLEILMAGGNAIDAAIAAAFALGVVEPNATGLGGEGMMVIYL
                     AENKTTIAIDYRSMAPLADMSKIKFGSEGHVAVAVPGTVAGLCTALKDYGTKSLAEVM
                     APAIRYARNGFIVSETLAQTIADRFDPISRNEALLQILAPEGLPLQTGDIFKNPDLAV
                     TLEKIAAGGPDVFYKGDIADAIAQDMAKNGGFITKADLAAYRAIKREPVRGTYRGYEI
                     VSAPPPVGGISVIEMLNMLECFDIASEQPLSPRNIHIMAEVMKRGFADNSAYVGDPDF
                     FQIPVTGLLDKEYARSRVQEIDLNKVTTSIKAGTPDEHPSTTHLSVVDRHGNMVALTQ
                     TISGFWGACVAVPGTGIILNNEMQNWSSRGPNSYAPGKRMRTTIAPTIIAKDGNPFVT
                     MGTPGAGRIISTMVILTVNLIDFDMGVQEAIESPRFYARDTEKLLSLESRIPKETQEW
                     LKSIGYSIKEYPPFDLFFGGAQAILVDPKTGIMHGGADPRRDGAVFGF"
     gene            complement(4421..>4985)
                     /locus_tag="PK105_07930"
     CDS             complement(4421..>4985)
                     /locus_tag="PK105_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOU61682.1"
                     /translation="PKGGDSFEGSESRNLNRVYPGKPDGTLTEQLAWAVMTLLKKEKV
                     DIAFDLHEAGPESRLANMIVANPKNLDLGALAVINLELEGINMKLEPSSDVFHGLSHR
                     EWGDETSASAFLIETPNPAQATDKGNPVEDPKHPLEERVAIHLATIEAILDAWNSDCL
                     PDKHIEFAMFPSWQAIKARGVGESLNW"
BASE COUNT         1309 a         1260 c         1070 g         1346 t
ORIGIN      
        1 gcacattagt tatgactcct attgtcgaat gagcaagccg cgtctcgagc acaccgatag
       61 tctctgggcg aatcgccatg cattctgcaa ctatagcctg agccccagcc ctatgtgcct
      121 ctcgtataaa ccatttgtgt tccattatcg aaggccgcct ctttgcagta ccatgttttc
      181 ttatctctct ctcgctcaaa tctggaagaa taatgcgggc tgcagagccg gtggtctttg
      241 catatactcg caaacctgcg gcagctagcc ctgccgcaat aagtcttgtc acagtggact
      301 tgccgcgcgt gccattgaca agaatgcgta tgggcacggc tttgaggcgc gcacaatgat
      361 ctttgtattc tactattgca gcgattgcta aacctatact tagtataaca ataaaagaaa
      421 tcacggcttt atatctgctc tccctctgac tccattgtct catgttacaa ggtagttgtt
      481 ctataggatt agcgccttag gacagctctt tggctagctc gattctcgat atagagatga
      541 ccatctcacc ggtcgcagga ggactcaata ttagaatgtt cccataggat atctcgatac
      601 cacagagaca tacgttatta ttacggtctg ctgaattgct cattacattg tacttaattt
      661 ctaccggcac cgatccaaat tctgggggtt tttgcaatac aatttgaaga aaattttgtc
      721 ctctttcctc atcgccgctc ccatgtccgc tttcatctgt taggagccag actacgactt
      781 tcctctggga agcaaggcaa tagcatccta tcttttgcac ctctctgggc acttttctca
      841 ataactcaga cgcgtcttcg atgatttcgg aactttttgc cagatcaaaa tctatgtctt
      901 ggtcggtact attcttccat cgtgagagaa acgatgaaac tctctccatg gctagatcct
      961 tggagagata aaccgtattc attctatgaa tatattgagc gatattgagc acaaaataca
     1021 gcgctttaat tcttttctat accgagcagc catttttgta cttcttcttt tgacgaaggc
     1081 aagccctcaa tattcaaagc aacggagtct cctatctctt cgtaagccga agccacaaga
     1141 gcctcaatcg cgcagagatg tataaaaacc ctctctgcaa gaggggcctt atcattgaag
     1201 atttgatcga tggtaggatc atcgctcggc tgtgctgggt tcagtgtttc cacaagaaaa
     1261 gctcgaatac ctagctttcc ccattcccaa tgcgaatagc ctgcaaactc atctcttgag
     1321 ctctcaaggt gaaaggattt ttcagtctcc tcttctattt gaagcacggc gagcgctgcc
     1381 acatcgagat attcaggcct tgtaatgata gaccacgcaa gagaagaacc tgggcttgct
     1441 tcgtgcagat ccagcgctgt tcgcacatgg ttctgcacga gtagccgagt gatagcatac
     1501 gcaatttgac ttgtgtagtt gcccgttgga tccccgggat attgacgatt tagatttcgc
     1561 acttcctgac cagataaaga agggaaagat tccgaagcat cgaagggaat aaaaaaacca
     1621 ggatctgggc tcttttcatg ggcaggattg gaaagcctag ctccataatg aagaatgggt
     1681 gcagcagctt tgcttgtcga ccatgttgca gccgaggcat ttgctcgagg cacaacaatg
     1741 agcttgccgc cagacactct gcaatgctcg attatccaat atgccgctag actccccgca
     1801 atttcgtttg catgagcacc ggctactata gcaaccgtat agtctgtcga ggaactatcc
     1861 tgtttttcag tagtttcttc cgaaatagat ctttcaggtg cgttcttcac atgcagtact
     1921 gggctgtctc cgataaaaaa aacaggtgtg tcaaaagcag ttccctctaa tgacgacaaa
     1981 aaagcagaca gggaaagaac cttttcgaca cctttccctg ccttcacttc tgacataaca
     2041 aacgctacgc catcctctgt ttgagcttgc gaaacaaaga ggccgagcaa aacaaaccaa
     2101 gcaagaaaga actttctcat tgcgcttcaa aaagactctt gagcacatta cctgcttcgg
     2161 cgatcgaagg gacttgaaca aggaagacat tgttctcttc gcacaaagaa gtaaaaaaac
     2221 cgtcattgtt gccgccttcg acgacaatta ccacatcaga agcagggaca cagagctcta
     2281 ttacctgatt cgaggcttct cctcggcgcg cggttccacc tgtatgtata gaaatgattc
     2341 gggtattatt tttgagggct tcagcaacga tgctttgcac gcgctcaatt tcctgctcaa
     2401 tgctcaaccc tgcggcacca agacctttgt tcgagacacc aagaacgagc actagcgtct
     2461 ttgcgtttgc tatagtctca ggctttgccg caatatcata ctcaagaccg agcttgagcc
     2521 tcgtgttggc caggacttta acaattgcgg catcggaagt ctgaccagct gatgttataa
     2581 atcctgggcg ctcaaaactc gccacaagag tcgatgggac agaatattgc gcaaaaatag
     2641 gcaatattcc taaaagcata aaacacacta ttgcgagaaa tctctttgta ctagaatatc
     2701 tataaatcat atgccacacc ttcaaaatcc gaagactgca ccatcgcgtc tagggtcagc
     2761 acctccgtgc atgattcccg tcttcggatc tacaagaatt gcctgtgctc cgccaaaaaa
     2821 gaggtcaaag ggcggatatt cctttatgct atagccgatc gatttgagcc attcctgggt
     2881 ttctttggga attctcgatt ccaaagatag gagtttttcg gtatcccgcg catagaatct
     2941 aggggattct atcgcttcct gaacacccat atcgaaatct atgagattca ccgtcaaaat
     3001 gaccatagtg gaaattattc ttcccgctcc aggggtcccc attgtcacaa aaggattccc
     3061 atcctttgct atgatcgtag gggcgatagt agtgcgcatt cgcttcccgg gtgcatagga
     3121 atttggaccg cgcgaagacc aattctgcat ttcattgttg agaatgatcc cagttccagg
     3181 caccgcaaca catgctcccc aaaatcccga aatggtttgc gtgagcgcca ccatgttacc
     3241 atgccgatcg acaacggaga gatgcgttgt ggacgggtgt tcgtcgggtg ttccagcctt
     3301 tattgaagtc gtgactttat tcaaatcgat ttcttggaca cgacttctcg catattcttt
     3361 atcaagaagg cctgttacag gtatctggaa aaaatcagga tcccctacat aggcagaatt
     3421 atctgcaaat cctcgtttca tgacttcagc cataatgtga atattcctgg gagaaagggg
     3481 ctgctcggat gctatatcaa aacattcgag catgttgagc atctctatta cggaaattcc
     3541 acctacggga ggaggcgccg atactatttc ataaccacga tatgtaccac gcacaggctc
     3601 acgcttgatc gctcgatacg cagccaggtc tgcttttgta ataaaaccgc cgtttttcgc
     3661 catatcctgt gcaatagcgt cggctatatc tcctttgtag aaaacgtctg gccctcctgc
     3721 agcgattttt tcgagagtaa cggctaagtc tggatttttg aatatatcgc cagtctgtaa
     3781 aggcaggcct tcaggcgcaa ggatctgtaa aagcgcttcg tttctcgaga taggatcgaa
     3841 ccgatctgca attgtctgcg ctaacgtctc gctcactata aatccattgc gagcatagcg
     3901 aatagcaggt gccatgacct cagccagact ttttgttcca taatccttga gtgcggtgca
     3961 aagacccgct accgtacctg ggacggctac cgccacatga ccttcactgc caaatttgat
     4021 tttcgacata tccgccagag gcgccattga acggtaatcg attgcaatag ttgtcttatt
     4081 ctctgcgaga tatatgacca tcatgccttc gccgccaaga cctgttgcat taggttctac
     4141 aaccccaagg gcaaaggctg ccgcaatggc tgcgtctatt gcattgccac cagccattag
     4201 aatttcaaga ccggcagcgg aagcgagcgg ctgagcggaa gccaccatgc cattgcgtgc
     4261 agtaatctca gcgttaattg tatcagcagc aaatatgggc agagcaaaga cgaaagcagc
     4321 caataaaaac aataagaatt ttcttacacc atgcttcatt cctaagccaa cactcttgtt
     4381 cttctgagcc atatcaaaaa cctcctcaat atcaattagt ttaccagttt agactttcgc
     4441 caacaccccg cgccttgata gcctgccagc taggaaacat tgcaaattct atatgcttgt
     4501 ctggcaaaca atcggaattc catgcatcca gaattgcttc aattgttgcc aaatgtatgg
     4561 ccacacgctc ttcgagagga tgctttggat cttctaccgg attcccttta tcggtcgctt
     4621 gggctggatt cggagtctca ataagaaaag cagatgcact cgtctcgtct ccccattctc
     4681 tatgagaaag cccatggaac acgtcgctcg aaggttccag tttcatatta atcccttcca
     4741 attccaggtt gatcaccgcc aaggcaccaa gatccaggtt cttcggattt gccacaatca
     4801 tattggcaag gcgtgattct ggtccagctt catgcaaatc gaaggctatg tccactttct
     4861 cctttttgag caaagtcata accgcccatg caagttgttc tgttagagtt ccatcaggct
     4921 taccaggata aacacggttg agattgcgag actcggagcc ctcgaaagaa tcacctcctt
     4981 ttgga
//
LOCUS       DAOOWY010000126         4953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_17131_length_4953_cov_11.721723, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000126 DAOOWY010000000
VERSION     DAOOWY010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17131_length_4953_cov_11.721723"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PK105_07935"
     CDS             complement(<1..620)
                     /locus_tag="PK105_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61683.1"
                     /translation="MRKKHVAYFVLLIIVALLFSGCDAMITNVYKTMNLGQPDPEKIA
                     QKNADVIIEESGITSGDGPSQDYVKKIAKDDQAKKAVIEKLDEVVNSPSATTEEKQAS
                     QALIINIELSSIDADIVAKNVNLSLGEFLKAAQDDQEVEPAAIIDKILPAELSNNETK
                     LAEFVDNLSALGGDVDKLAQIIDENDKTAAEGLDIATIAQTAAIIK"
     gene            complement(764..2239)
                     /gene="mtaB"
                     /locus_tag="PK105_07940"
     CDS             complement(764..2239)
                     /gene="mtaB"
                     /locus_tag="PK105_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOU61684.1"
                     /translation="MAMLRVAFQTLGCKLNQLETESIADKFLDAGAEIVPFHEEADLY
                     IVNTCTVTSKAEQKARHDIRSAIHKVRNTTVIATGCYAQMDSEDLSLLDERVIVISGD
                     LKASLLLLPQWLLANWQGHGELFHALLEWRDEILAAAKGSEALSNESGKTNTIDLSLR
                     NRFAFHPERFLMHSRPALKIQDGCNNRCTYCRVCLARGPSVSLPVEDALSRVIDLERA
                     GKAEVVLTGVNLAQYRAGGLDFAGILASMIKETSNIRFRISSYEPENIDEAFLATFAH
                     PRVRPHLHLSVQSGCTETLRRMGRPYSAQDVRFAVQNLRSVRSDPFLAADIIAGFPAE
                     TESEFENTIALLSELDFAWIHAFPFSPRQGTRAADMRPRIPERIACERVDRLLELAKH
                     GKRSYIERWLGHEVEMVIEHEASADKHNGDENRATDYASARIVGTTENYLKAMIEASE
                     RVEQSIMPGRAGDSVKVVLHSKSESALADGAIDVLATISSK"
     gene            complement(2258..2938)
                     /locus_tag="PK105_07945"
     CDS             complement(2258..2938)
                     /locus_tag="PK105_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU61685.1"
                     /translation="MIYQANRIGRIALIGMFFLSIWISGLFGQSPSYSISVPTGMTGS
                     DLLRMSQEGKHNDVALLAPQAIAKNPQDVDAYIGLAWSLNALKEYAKARDIAKQGYSK
                     FKDPRLAQALGEAQYYLGENEAALANLQQYLAKFPEGSKTALTFYLCGELFVRMSKLN
                     HADIAFSTALQYNPSNARWWARLGWVRERTARYIPALKAYEQAISLNPNLEDALNGKS
                     RMLAKIED"
     gene            complement(2935..3288)
                     /locus_tag="PK105_07950"
     CDS             complement(2935..3288)
                     /locus_tag="PK105_07950"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU61686.1"
                     /translation="MAEEVKLSVVDEEEVDTVLGSEIEFEGDIESSKSLMIKGKVSGN
                     INCSAELYITEQAEVRSTIHAATVVIRGKVYGDISADSLIAVLDWGHVEGCLVAPDIY
                     LSPNCVFKGTTVIKS"
     gene            complement(3320..4216)
                     /gene="rsmA"
                     /locus_tag="PK105_07955"
     CDS             complement(3320..4216)
                     /gene="rsmA"
                     /locus_tag="PK105_07955"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOU61687.1"
                     /translation="MSQNEINYDSPASIQAFLVQNGLAVSKRLGQNFLIDRSVRSRIV
                     KALEVEEGMHVWEIGPGIGAMTTLLLDEGVRLSAFEIDYGFVRVLEGLFSANENFSII
                     KGDVLKTLCSQKEPPERIFGNLPYNSAFAIISDILEMEFLPQRMIFTLQKEAAQRMVA
                     GPGTKDYSSLSVLCASVCDVRILFDIGSSAFWPRPRVTSSVVQLIPKKKSMSASERKD
                     FSDFVRAVFSSRRKTLRNTLRFWIKQRSPSLREEEIDTRLRAILEELLIDPSIRAEVL
                     TPDELFAVYRQLHALLASSTSC"
     gene            complement(4253..>4953)
                     /locus_tag="PK105_07960"
     CDS             complement(4253..>4953)
                     /locus_tag="PK105_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOU61688.1"
                     /translation="PGPSLLRSVFMLSCAEIAIEIDRPQSGFAILSLAAILLALFSPS
                     SINSLSSIYSFSAMAGLMAFGSRFLIILRHWLPDWLARAFSASFAAVCGTAPVSLLTF
                     GTFIPAGIFAATAAAPVMLVFMWIALVGGLIAVPLAFVSRITAPLLEALQSVLTWILA
                     SSASFPIFNVGSIPMLRILSCLLVVCFITLIYAIPWIVWCKSQRKIEELQGRMPFLRS
                     KNMYAILNPPDEAQ"
BASE COUNT         1335 a         1242 c         1095 g         1281 t
ORIGIN      
        1 aatttgatta tggccgctgt ctgagcaata gttgcaatat ctagcccttc tgcagctgtc
       61 ttatcatttt catcaattat ttgcgcaagc ttgtcgacat caccgccaag cgctgaaagg
      121 ttgtctacga attcagctag ttttgtttca ttgtttgata gctcagctgg caagatttta
      181 tcaatgatgg ctgcgggttc cacctcctga tcatcttgag ccgcttttaa aaattcccct
      241 aaagagaggt ttacattttt tgcaacaatg tccgcatcga tactagacaa ttcaatgttt
      301 ataataagtg cttgcgatgc ttgcttttct tctgtagttg cactaggaga attgacaact
      361 tcgtcaagtt tttctataac tgcctttttt gcctgatcgt cttttgctat tttcttgaca
      421 tagtcttgcg atggtccatc ccctgatgta atcccagatt cctcaattat gacatcagca
      481 tttttctgag caattttctc tggatccggc tggccaagat tcatggtctt atacacattg
      541 gttatcattg catcacagcc cgaaaagagc aacgcgacta ttatgagtaa cacaaaatat
      601 gcaacatgtt tctttcgcat aaatacctcc tttttgtcat tcatgcgatc tcgacttaac
      661 tccacaactt gacaactcta cgtaatatat tctagctcaa tttggcaata aatcaagaag
      721 caaaaaacgt agagcccgaa taaaactggt cttggaagcc ttattatttt gacgaaattg
      781 tcgccagcac atctattgcc ccgtccgcca atgcgctttc gctcttgcta tgcaacacta
      841 ccttgaccga atctcctgcg cggcctggca ttatactctg ctctaccctc tccgacgcct
      901 cgatcatagc tttaagataa ttctctgtgg taccaacaat tcgcgcagat gcataatcag
      961 tagccctatt ttcatcgcca ttatgtttat cggcactggc ttcatgttcg ataaccatct
     1021 cgacctcatg cccaagccat cgctctatat aagagcgctt cccatgcttg gccagctcaa
     1081 gaagcctgtc aacgcgctcg caggcaattc tttctgggat acgcggtctc atgtctgcag
     1141 cgcgcgtacc ttgtcttggg ctaaaaggaa aagcgtgtat ccaggcaaaa tcgagctcgg
     1201 aaagcagggc tatagtgttc tcaaactcag actccgtctc tgcagggaag ccggctatta
     1261 tgtccgctgc caggaaagga tcgcttcgga ctgaacgcag gttctgtaca gcgaatctaa
     1321 catcttgggc ggagtatggt cttcccatgc gtcttagtgt ctcagtacag cccgattgca
     1381 ccgagagatg caagtggggg cgaactcgcg ggtgggcaaa agtcgccaga aaggcttcat
     1441 cgatgttctc aggctcataa cttgagatcc gaaacctgat attgctcgtc tcttttatca
     1501 tagaagcaag aatgcccgca aaatccagcc ctcccgctcg atactgcgcg aggtttacgc
     1561 ctgtaagaac aacctccgcc ttgccagccc tctcgagatc gatcacgcgg gagagtgcat
     1621 cctcaaccgg caaggatacc gaaggacccc tagcaaggca cacgcggcaa tatgtgcagc
     1681 ggttattgca tccatcttga atcttgagtg caggacgaga atgcataagg aatcgctctg
     1741 ggtggaacgc aaagcgattg cgcaaggaca ggtctattgt attggttttg cccgattcat
     1801 tcgacagcgc ctccgatcct ttggccgcag ctagaatttc atcgcgccat tctagtaaag
     1861 catggaaaag ttcgccgtgg ccttgccaat ttgccaaaag ccattgggga agcaaaagaa
     1921 gcgaggcttt caggtctccc gaaataacaa tgactctttc gtccagcaaa gaaagatcct
     1981 cggaatccat ttgagcataa caacctgtgg ctatcactgt agtgttacga accttgtgta
     2041 tggcggagcg gatgtcatgc cgcgcctttt gttcagcttt ggatgtcaca gtacaggtat
     2101 ttacgatata gagatcggcc tcttcatgaa atggcacaat ctcggcgccc gcatccagga
     2161 atttgtccgc gatggattcg gtttcaagct gattgagctt gcagccaaga gtctgaaatg
     2221 ctacccgtaa cattgccatt cgggaatttc tccgccctca atcctcgatc ttcgccagca
     2281 tgcgcgattt tccgttgagg gcatcctcca ggttaggatt taggcttatg gcttgctcgt
     2341 aggccttgag cgcaggaata tagcgagccg ttctttccct gacccatcca aggcgtgccc
     2401 accatcgagc gttgcttgga ttgtactgca gagccgtgct gaatgctatg tcggcatgat
     2461 ttaatttgct cattcgcaca aaaagttcac cacataaata gaatgtaagg gcagtcttgc
     2521 ttccttctgg gaattttgcc agatattgct gtaaattggc cagtgcggct tcgttttctc
     2581 caagataata ctgagcttcg cctaaggcct gggcaaggcg cggatccttg aacttagaat
     2641 agccttgctt ggcaatatcc ctggcttttg catattcctt taaggcattt agactccaag
     2701 caaggcctat gtatgcatct acatcctgag gattcttcgc gattgcttgt ggagcaagaa
     2761 gagcaacgtc gttatgcttt ccttcttggc tcattcgaag aaggtccgag cccgtcatgc
     2821 cagttggtac acttatcgaa taagatggcg actgaccaaa tagtccggaa atccaaatag
     2881 ataaaaagaa catcccaata agggctatcc gcccaattct attagcctgg tatatcatga
     2941 tttgataacc gttgtccctt tgaaaacgca atttggcgaa agataaatgt caggggcaac
     3001 caggcaccct tctacatgtc cccaatcgag cacggctata agggaatctg cgcttatatc
     3061 accatatacc ttcccgcgga tcacgactgt ggcagcatgg atggtcgatc gcacctcagc
     3121 ctgctcggtg atgtagagct cggcgctaca attgatattc ccactcactt ttcccttgat
     3181 catgagggat ttcgaggatt caatatcacc ctcgaattct atctccgagc cgagcactgt
     3241 atcgacttct tcctcgtcga ccacgctgag ttttacttct tcagccatct tgtcaatata
     3301 ataaagatct aggctaatct cagcaagaag tagaggatgc cagcaaagca tgcaactgtc
     3361 gataaactgc aaaaagctca tccggtgtaa ggacctctgc gcggatggaa ggatcaatta
     3421 gcagctcttc caagattgca cgcaatcgag tatcaatttc ctcttcacgc agagaaggag
     3481 agcgctgctt tatccaaaag cgcaaggtat ttctaagtgt tttcctccga gatgaaaaaa
     3541 ccgcacgcac aaaatcggaa aaatccttgc gttctgaggc ggacatcgat tttttctttg
     3601 gaataagctg aacaacagag ctagtcactc ttggccttgg ccaaaatgcg ctcgaaccaa
     3661 tatcgaaaag aattcgaaca tcgcataccg aggcacagag cacgctgagc gaggaataat
     3721 ccttagtccc cggtccggct accattcgct gtgcagcttc tttttgaagg gtaaaaatca
     3781 tgcgctgagg cagaaattcc atctcaagaa tatccgaaat tatagcaaac gcagaattat
     3841 atggtagatt tccaaatatg cgctcaggtg gctccttctg cgagcatagc gtttttagaa
     3901 catccccctt tataatagaa aaattctcat ttgcagaaaa aagaccttcg agtaccctca
     3961 caaatccata atcgatctca aaagcagaca gtcgcacccc ttcatcgaga agaagtgtgg
     4021 tcattgctcc aataccgggg ccaatctccc atacatgcat gccctcttct acttccaatg
     4081 cttttacgat gcggctgcgc accgagcgat ctatgagaaa attctgtccc aagcgcttac
     4141 tgacggcaag tccattctgc actagaaaag cctgaatcga agcgggagaa tcgtaattta
     4201 tttcattttg gctcatagaa atcgttaagc ccccattcat taagacctta gcttattggg
     4261 cttcgtctgg cggattcaga attgcataca tatttttcga gcgaagaaag ggcatgcgcc
     4321 cttgcagctc ctcaatcttc cgctgggact tgcaccagac aatccatgga atagcataaa
     4381 tcagagtaat aaagcagaca acgagcagac atgacaagat ccttaacatt ggtattgagc
     4441 ctacattgaa aataggaaaa gaagcgcttg atgcaagaat ccacgtaagg acagattgca
     4501 aggcttcaag taatggcgct gttatgcgac ttacaaaggc taaaggaacc gcgattagcc
     4561 ccccaaccag cgcaatccac ataaaaacta gcattactgg tgctgcagcc gtcgccgcaa
     4621 agataccagc aggaataaag gttccaaagg tcaagagcga cacaggagca gttccacaaa
     4681 ccgctgcgaa ggatgcggaa aaggccctgg ccagccaatc cggcagccaa tgcctcagaa
     4741 taatcaaaaa tcgcgaacca aatgccataa gccctgccat ggcgctgaag gaatagatgc
     4801 tcgaaaggga attgatcgag gaggggctaa acagtgcaag caaaatagca gccagagaga
     4861 gaattgcaaa gcctgattgc ggcctatcga tttctatggc aatctccgcg cagctcagca
     4921 taaagacaga tctcaaaagc gagggcccag gcc
//
LOCUS       DAOOWY010000127         4937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_17207_length_4937_cov_10.815444, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000127 DAOOWY010000000
VERSION     DAOOWY010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17207_length_4937_cov_10.815444"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..4352)
                     /locus_tag="PK105_07965"
     CDS             complement(15..4352)
                     /locus_tag="PK105_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61689.1"
                     /translation="MDSVTELSRYTESILKPIRSEISYDGKTIKLDRSAALKILLDVK
                     DSSSPIILSGGAGVGKTAVIKDFYDAFKESHPLFVLKATQFRNISHINQLFKDYGEIT
                     ATDFINEHKDIGKKYLVIDSAEKLAEIEDREIFRIFLSTLLENGWTVIFTVRYSYLDD
                     LRFLLKEIGVSFTSVNIPGLTRAELEQIASDHGIRLPKNERLKELLETPIYLNIYLQN
                     YTSMRDDVNYSDFRNIIWEKHIQDSSHHSGNLHRRREECFLMIARKRANEGNFFVKSE
                     NCDQEALEKLEADEIIQHDPNAGGYFITHDIYEEWALDKIIEQAFIGASDYGNFYQTI
                     GTSLPVRRALRNWLSLKLLMNDESAKRLIEFTVKDSGIDGHWKDEILVAVLLSDYSRI
                     FFERFEDELLEGLDEESSGVVKALGDGFKYENRLLHRILFLLRIACKAIDEDLLRRLG
                     LTRAGAVLLQTVFTKPKGSGWDSTIAFINKNKGKLRFRYMNAVLPVLDDWNLSHKQGE
                     TTKNASQVALFYYEELTKQESFYFSSRDDTKDKLIRAILNGSGEIKPELRAIVNEIVT
                     RKDTSHRGRYYELVQAILSSIISSSVISEHLPEEVIKLAELFWVYEPKEDQYPFSSAR
                     IDIEQYFGLSEGHHDYYPASAFQTPILPLLQTYPQKTVDFILSFTNKSIEYFAKSELG
                     QNEVKTINVVIDDSGNTVRQYICDRIWNLYRGTQVAPPLLESIHMALERWLLEVAKLA
                     DSKITESWCLYLLKNSRSASITAIVASIVLAEPSKLFNVAKLLFRTKDLFFYDTARMQ
                     LDTTAKDLYAMSYDPTGLFMNERLETCDDKHRSNSLENLALQYQLSSTEGEEEEMLRQ
                     RQEIIWKIFDDHYKRLPEKSNETEDDKTWRLCLARMDRRKMKITTEQKDDKVFISFNP
                     EIDPELKKYSEDSLARNSESMKYMPLMLWARNRFERNEDYKKYPQYEDDYKLAISESK
                     KIVEGLEDDKSEGQSFTLFYRSVPSYVCAVLIRDFLDKLDSEEREFCKDVIIKQAAAP
                     LGDAYGYQIGDGIDAAINALPLLLNLFPDDAADIKMILLFTLFDSNPIGAGQQFSDYT
                     IGAIFDVLWNESHADANSIFIGFLRLKPLYDKLLDSYQQEYFRQEKYDLPKSKVLEAF
                     YEKYHEEIARVTNNEINYSEIDVNQIDLDTLVTAFRLLPIEISDEVHKTFMRQVFPIF
                     SKEVFKDRHKRDKEHQFDYMARQTFLEKLAYIVLSTKAEEIESYLRPFLDDFEGSDGA
                     EDFFREFVNAEDRVAQYEQFWIVWKLFYPKIVALAKGKGSKFYSKEIIRNYLLAGDYW
                     REDAREWHTLKDREKSFFKKVSEDIGSSPSVLYSLAKLLTDIGSAFGDDGILWISDMI
                     KNNPGLSSKELEVNTVYYLENLVRSYIFKNHHKVRTTLRLKNQIMIILDFLLEKGSVT
                     AYLLREDIL"
     gene            complement(4437..4832)
                     /locus_tag="PK105_07970"
     CDS             complement(4437..4832)
                     /locus_tag="PK105_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61690.1"
                     /translation="MEEVNWNNFKAKFHDHEQTAFERLCYLLFCKEYGKDTGIFRFNN
                     HAGIETDPIEKDGQVIGWQAKFYDTRLSEHKDELIQSIDTTLARHPEVNKIIFYTNQE
                     FGQGKKKSDPQYKTDIENHARGKGINIEW"
BASE COUNT         1291 a         1159 c          980 g         1507 t
ORIGIN      
        1 ttcgcagatc ttttttataa tatgtcttct cgtagcaaat atgctgtgac ggaacctttt
       61 tccagaagaa aatctaaaat gatcataatt tgatttttta ggcgaagcgt tgttcggact
      121 ttatgatgat ttttaaaaat gtaacttctt acaagatttt ccagataata cacggtattc
      181 acttccagct cttttgaaga caagccagga ttgttcttga tcatatcgct gatccaaagt
      241 atcccatcgt ctccaaaggc actccctatg tcagtcaaaa gttttgccag cgaatacagg
      301 actgaagggc tgctccctat atcctcggat acttttttaa agaatgattt ttcgcgatcc
      361 tttagagtat gccattccct cgcgtcttct ctccagtaat ccccagccaa gagataatta
      421 cgtattatct cctttgaata gaacttggag cctttgcctt tagccagggc cactatcttt
      481 ggataaaata gtttccagac aatccaaaac tgctcatatt gcgcgaccct atcttccgca
      541 ttgacaaatt ccctgaagaa gtcctcggca ccatctgaac cctcgaagtc gtccaaaaat
      601 ggcctaagat acgattctat ttcttcggcc ttggttgata ggacgatata ggcaagcttt
      661 tccagaaatg tttgccttgc catgtaatca aattggtgtt ctttatctct tttatgcctg
      721 tccttgaata cctccttgga aaaaatcgga aaaacctgtc gcatgaatgt tttgtgaacc
      781 tcgtccgata tttctatagg caaaagcctg aatgccgtaa ctagagtatc aagatctatt
      841 tgattgacat caatttctga gtaattaatt tcattattag tgaccctagc aatttcttca
      901 tgatactttt cataaaacgc ctcaagtacc ttggatttag gaagatcgta tttttcttgt
      961 ctaaaatatt cttgctggta agagtccaat agcttatcgt ataacggctt cagccgcagg
     1021 aaccctataa atattgaatt tgcgtcagca tggctttcat tccataatac gtcaaaaatt
     1081 gctcctatgg tgtagtccga aaactgttga cccgcgccga tcggatttga gtcgaacaac
     1141 gtaaaaagaa gtatcatctt tatatcggct gcatcatcag ggaacaaatt cagcaacaaa
     1201 ggcaacgcgt tgatagctgc gtcaatgcca tcgccaatct gatagccgta ggcgtctccg
     1261 agaggcgctg cggcttgctt gatgatcaca tccttgcaaa actctctttc ttccgaatct
     1321 agcttatcca ggaaatcccg aatcaatacg gcgcatacat aagacggaac cgagcgataa
     1381 aacaaagtga aactttggcc ttcacttttg tcatcctcca atccctcaac aatcttcttc
     1441 gattctgaaa tggctagctt gtaatcatct tcgtactgag gatatttttt ataatcttcg
     1501 tttctttcaa atctgttcct cgcccataac atcaatggca tatacttcat tgattccgag
     1561 tttctggcca aggaatcttc gctatacttt ttcagctcag gatcgatctc cggattgaaa
     1621 cttataaaaa ctttgtcgtc tttttgctcc gtcgtgattt tcatctttcg tcggtccatg
     1681 cgcgccagac acagtctcca ggtcttgtcg tcttctgtct cattggactt ttcaggcaac
     1741 cgtttgtagt gatcatcaaa tattttccaa atgatttctt gtctctgcct gagcatttcc
     1801 tcttcttcac cttctgtgga agatagctga tactggaggg caagattttc aagcgagttc
     1861 gagcggtgtt tatcgtcgca ggtctcaagg cgttcgttca taaataggcc tgtcggatcg
     1921 tagctcatgg cataaagatc cttggcggtc gtgtccaact gcatccttgc tgtatcgtaa
     1981 aaaaacaaat ccttggtgcg aaacagcaat ttcgcaacat tgaatagttt tgatggttca
     2041 gcgagaacaa tacttgcgac aattgccgta attgaggctg agcgggaatt cttgagcaga
     2101 tacaaacacc agctttcggt gatttttgaa tctgccaatt ttgcgacttc aagcaaccac
     2161 cgctccagtg ccatgtggat ggattcgagt aaaggaggtg ctacttgagt gccccgatat
     2221 aaattccaga tcctatcgca tatatactgc ctaacggtat tgccggaatc atcgataacc
     2281 acattaatag ttttcacttc attttgaccg agttctgatt tcgcaaaata ctcgatcgat
     2341 ttgtttgtaa aggaaaggat gaagtcgacc gttttctgag gataagtctg cagtaacggc
     2401 aagatggggg tctggaaagc gctcgcgggg taataatcat gatggccttc tgacaaaccg
     2461 aaatattgct cgatgtcaat cctggcgctg gaaaacgggt attgatcctc tttgggctca
     2521 taaacccaaa agagctcagc aagctttatt acttcctcag ggaggtgctc agagatcaca
     2581 gagctactga taatcgacga aaggattgcc tgcacaagct cgtaatatcg gcccctgtgg
     2641 ctggtgtctt ttctggttac gatttcattt acaatggccc ttaattcggg tttgatctcg
     2701 cccgaaccgt taaggattgc ccttataagc ttgtctttcg tatcgtcgcg ggagctgaag
     2761 tagaaacttt cttgtttagt cagctcctca taataaaaca atgctacttg acttgcattt
     2821 ttcgtggtct ctccttgctt atggcttagg ttccaatcat ccagcacagg aagaactgcg
     2881 ttcatatatc tgaaccgtag ctttcccttg tttttgttga taaaggcaat tgttgagtcc
     2941 cagccgcttc cttttggctt agtaaaaacg gtctgcagca agaccgcccc ggcccttgtc
     3001 aacccgaggc gtctcaataa atcttcatct atagctttgc aggcgatccg caagagaaag
     3061 agaatcctgt gcagtaacct gttctcgtat ttgaagccgt caccgagagc cttcacgacc
     3121 cctgaagact cctcatccaa tccctccagc aactcatctt cgaagcgttc aaagaaaatg
     3181 cgggaataat cagataaaag caccgcgacc aatatttcgt ccttccagtg cccatctata
     3241 ccgctgtctt tgacagtgaa ctcgatcaaa cgcttggcgc tctcgtcatt catgagaagc
     3301 ttaagggaga gccaattcct gagtgcccta cggacgggaa gcgatgttcc tattgtctga
     3361 taaaaatttc cataatccga ggcaccaatg aacgcttgtt cgataatttt gtccaaggcc
     3421 cattcttcat aaatatcgtg agtgataaaa tatccaccgg cattggggtc atgctggatg
     3481 atctcatcgg cctctaattt ttcaagtgct tcctgatcgc aattctcaga cttaacgaaa
     3541 aaattaccct cattcgctct cttccgagct atcatcagaa agcattcttc tctccgtctg
     3601 tgaagattcc ccgagtggtg gcttgagtcc tgaatatgct tctcccagat aatgttgcgg
     3661 aaatccgaat agttaacatc atctcgcatg ctcgtgtagt tttgcaggta tatattcaga
     3721 taaatcggtg tctctaagag ttctttaagc ctctcatttt tgggaagtct gatgccgtga
     3781 tcagaagcaa tctgttctag ctccgcccgt gtgagaccag gtatgttgac agaagtaaaa
     3841 ctgaccccga tttccttcaa caggaagcgg agatcgtcca aatagctata tctgaccgta
     3901 aaaatgaccg tccatccgtt ttccaaaagg gtggagagaa atattctgaa aatttcgcga
     3961 tcttcgattt ccgccagctt ctccgctgaa tcaatgacga ggtatttctt tccaatatcc
     4021 ttatgctcgt tgatgaagtc tgtcgcggtg atctctccgt aatctttgaa tagctgattg
     4081 atatgggaaa tgtttctgaa ctgagttgcc ttcaatacaa acagaggatg agattccttg
     4141 aacgcatcat aaaaatcctt gatcacggct gtcttaccga cgccggctcc gccgctaaga
     4201 attatgggag aagagctatc cttcacatcc aagaggatct tcaaagccgc agacctatcc
     4261 agctttattg ttttgccgtc atacgatatt tccgagcgga tcggctttaa aatagattcg
     4321 gtatatctcg atagctcagt gacagaatcc aaaatccctt tcttcaggct gaagaaatgc
     4381 tgagctatgc tagaattctg ctgacagaca aacggagact cgaaaaagct tgatgttcac
     4441 cattcgatat tgatgccttt tcccctggca tgattctcga tatccgtctt gtattgagga
     4501 tcagactttt ttttgccttg gccaaattcc tggttcgtat aaaaaatgat cttgtttacc
     4561 tcgggatgcc tcgccaaggt agtatcgatt gactggatta gctcatcctt atgctctgat
     4621 agcctcgtat catagaactt ggcctgccag ccgattacct ggccatcttt ttctattgga
     4681 tccgtttcaa ttccagcatg attattgaaa cgaaaaatac cggtgtcctt gccgtactcc
     4741 ttacagaaca gtagatagca aagccgctca aaagccgttt gctcgtgatc gtgaaacttc
     4801 gctttgaaat tgttccagtt cacttcttcc attgtccatt ccccttttta caatcccata
     4861 tcccttaaag aaccttattc tatcactctt ttcttggatc ccaaacaagc taactggtta
     4921 tctctccagc ccagagt
//
LOCUS       DAOOWY010000128         4923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_17263_length_4923_cov_17.450698, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000128 DAOOWY010000000
VERSION     DAOOWY010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17263_length_4923_cov_17.450698"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(425..817)
                     /locus_tag="PK105_07975"
     CDS             complement(425..817)
                     /locus_tag="PK105_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61691.1"
                     /translation="MITIITDPCDRSHLAHGMSPVATGSGGPSGLEDSPYTIARENGQ
                     RAESPDQERSSLHSGGRALERQTLPHARTRVPELLLSRISINLAAQSIGSMGALITSI
                     MNPGNHAASCPIGSEPLGEITDTILRRL"
     gene            976..1209
                     /locus_tag="PK105_07980"
     CDS             976..1209
                     /locus_tag="PK105_07980"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOU61692.1"
                     /translation="MRTLSYRITLRKEPEGGYTVLVPALPGCVTYGKTVEEAIEMARD
                     AINGYIESLIDDGEAIPLEEDLIECRLTVEAHA"
     gene            1202..1429
                     /locus_tag="PK105_07985"
     CDS             1202..1429
                     /locus_tag="PK105_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOU61693.1"
                     /translation="MPKLPSLSPQKVIAILEKKGFVLKRVTGSHYIFAHPETKRRVTV
                     PYHSNQDVARGTLIQILEDAGIKREELQDLL"
     gene            1996..2868
                     /locus_tag="PK105_07990"
     CDS             1996..2868
                     /locus_tag="PK105_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61694.1"
                     /translation="MDSDYLLDENGAKIRAGTNGNIALDASENGSIDLRATGLGDRNI
                     RIQGHIASWDQANDFITFESKIKANDGLEVGSIKPLISNHNIDIESAVINLRATGPGN
                     RNIRIQGHITSWDQANDFIAFESKIKANEGLEVRSIRPLESTKDIDIDSGRDGYINVL
                     GKIMGGIEGAEPIKFGTKIIAENGLDAYDGLNIEGKDAYCKISLVKFRENAESTQIQE
                     GLKIELKTPRIIGMKKSIIEGRCNLIITGEKILIESKSTRGRSKQTTYIDLVSALAKI
                     QSDIARLNERVGIE"
     gene            3111..4610
                     /locus_tag="PK105_07995"
     CDS             3111..4610
                     /locus_tag="PK105_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61695.1"
                     /translation="MADVSEVLKKMNLISKDSADSIRSVEEIIGGISAMISYAGVVKS
                     ILDKCGFFPKEDMLAKKLDEILDKFNEVLDLLEIGTKISDDLDKARTIATVMGPVRTS
                     MILLQAYLLDENSSNINTKEIFSDPLNAAVTLAGNAFWRGIYAEELAYDMENYALRHD
                     PCYWTYKAKPPDASGKISAVFDYKQTLPAFLEAITARLAMLVALEPGFRSNEAYRREL
                     LDYSDKLIEVHNRIIQNGIIPLNGPGEYALFCSYTDEKTPSGNVCYVISWRKNGCKYG
                     VVDLYSTTTKVSNYPLPFFLELENPPPNFQALSSDYIILNNDGNVLILIPIGKLDTCD
                     LAYWFGLYGVSELYWDACDSVRFFFEVVFKPKIIIRTIKLWQDLYSELGLPEVWRTIN
                     HLRWLAGYDPLELPDIPCTTISLKESYFIELFKLFKFYQCIRRSLRNIYSIYNIVKIR
                     ASFILAYKYPDPTSINGILKAISVSISMREIASELYVLPPISLSRLYED"
BASE COUNT         1507 a          928 c         1158 g         1330 t
ORIGIN      
        1 aggattgaca gaagaagagg ctgtctccaa tctcaagaaa ggccttgaag agcatatcaa
       61 gttctcctgg agctggtacc caaggggcga aagctctcat ttcttgacat agaggtggaa
      121 cgatttgctc aagctcccag tagtctcggc taaggaatct atcaaggctc tggagcattc
      181 tggaacgaga agagaaatct ggttgtgaca gtgcccaatc acccctgagc tggcgaaggg
      241 caccccgatc tcaatcatga agcaagctag gttggagaga aaggagttta tttcagcgct
      301 gaataggtga ggcgaaacga tgggtaatcc tcctggcagg gcggcattta aaactttttt
      361 cagaatagct cttggctttg ctcatttctc tgcttcggat ttgtcataat agcaatcatg
      421 aaccttagag ccgtctcagg atcgtgtctg ttatttcccc caatggctcg ctgcctatcg
      481 ggcaggatgc ggcatgattg cctggattca tgatggaagt tatcagggcg cccatgctcc
      541 ctatcgattg agcagcaagg ttaattgaga ttcgggataa tagtaattcg ggtactctag
      601 ttctggcgtg aggcaatgtc tgtcgttcca gagctcgccc acccgagtgc aacgaagaac
      661 gctcttgatc tggggactcc gcgcgttgcc cgttctcgcg cgcgatagta taggggctgt
      721 cctcaaggcc ggacggcccc cctgaccccg tggccacggg ggacatgccg tgggcaaggt
      781 gggaacggtc gcatggatcc gtgataatag taatcatgga gccctggttc cgtcttgggg
      841 caatgtctgt cgtttccttg gagctctcac ccctgctata tcgagatatt ggcggggttc
      901 ttgctgcaac gctgacctga gcgatgccgc aatagtcgca agttatattt cgttcgtcat
      961 ggatctatat caaatatgag aaccctgagc tatcgaatca ccctgcgaaa ggaacctgaa
     1021 ggcggctata ccgttcttgt ccctgcactt cccggatgcg taacttatgg aaagacagta
     1081 gaagaggcca ttgagatggc cagggatgca atcaacggct atattgaaag tctgatagat
     1141 gatggcgagg caattccctt agaagaggat ctcatcgagt gcaggctaac agtggaggct
     1201 catgcctaag ctgccatctc tttctccgca aaaagtgata gcgattcttg aaaagaaagg
     1261 cttcgtacta aaaagagtta cggggagcca ttacatcttt gctcacccgg aaacgaagag
     1321 aagagtcaca gttccttacc atagcaacca ggatgtagcc agaggcactc tcatacagat
     1381 tctggaagat gcgggaataa aaagagaaga gctacaagat ctgctttgac taaatgaaat
     1441 tcctactttg atctggttta tctataatgc agcgtggctt tatattcgtg ataggcagcg
     1501 atcagggagt tctggatccg tcatgagaca atgtctgtca tctctagagg gctcgcccaa
     1561 ccgagtgcag cgaagaacgc tcttgatctg gggactccgc gcgcgcttcc cgttttcgcg
     1621 cgcgaggact ggaggggctg tcctcaaggc cggacggccc ccctgacccc gtggccacgg
     1681 ggaacctgcc atgagcgagg ggatgctatt catctcgaat cggaaaagcc tatgcatcta
     1741 aatacttgca gcactttggc agagcgagca tatggctgca atggatacgg ttgcagagaa
     1801 tgtggatatc ctatagaaat agtcgtaata ataggatgtt aattttatat aataaacgat
     1861 ttcgggactc tacaaaacaa cagcgtaacc gaaacaatta aatctacaga aatcaaaata
     1921 tttaacaaat aagaaaggat cgcagtctaa atttaattgt ttaatctgca tatatttaaa
     1981 tgatcggtga tgctaatgga tagcgattat ctcctagatg agaatggcgc aaaaataagg
     2041 gctggtacga atggaaacat tgctctcgac gctagtgaga atggttctat cgatcttagg
     2101 gcaactggtc taggagatcg gaatataaga atccaaggac atatcgcgag ttgggatcaa
     2161 gccaatgact ttataacttt cgaaagcaag attaaggcaa atgatggatt agaagtaggg
     2221 agtataaagc cattaatatc aaatcataat attgatatcg aaagtgcagt aatcaatctt
     2281 agggcaactg gtccaggcaa tcggaatatc agaatccaag ggcatatcac gagttgggat
     2341 caagccaatg atttcatagc tttcgaaagc aaaatcaagg caaatgaagg tctggaagta
     2401 aggagcataa ggccattaga atcaactaag gacatcgata tcgatagtgg tagagatggc
     2461 tatattaatg ttctgggaaa aattatggga ggtattgaag gtgctgaacc tataaaattt
     2521 ggcacaaaaa ttatcgcaga aaatggtcta gatgcttatg atggtttgaa tattgaaggc
     2581 aaggatgcgt actgcaaaat ttcattagtt aaatttcgag aaaatgcaga aagcactcaa
     2641 attcaagaag gattaaaaat tgagctaaaa acaccaagaa ttattggcat gaagaagagc
     2701 atcatcgaag gaagatgcaa tttgattata acaggcgaaa agatacttat cgaatcgaag
     2761 tcaactcgag ggcgatctaa gcaaacaact tatatagatt tggtatctgc tctggctaaa
     2821 attcagtctg acatagcaag attaaatgaa agagtaggca tcgaatagag acaatcgatc
     2881 aaagtctgtt gcttctgacg aataaaacct agagataatc tcaaaatatg taggagacat
     2941 caaagacagg tagtaatcga aggggaaaat agtttgaaga tcaatgtatc aggacataat
     3001 tcaagtgaac tcgttagcta ttccataaag tatcaaaaaa atggatgaat aaaaattggt
     3061 aggtaataga aggtatttta tcaatattat taatcccaag agggatgata ttggctgatg
     3121 taagtgaagt attaaaaaaa atgaatttaa taagcaaaga ttcagcagat tcgatccgta
     3181 gtgttgaaga gattattggc ggaatatccg ccatgatttc ttatgcggga gtagtcaaga
     3241 gcatattaga taagtgtggc ttttttccaa aggaagacat gttagctaag aagctagatg
     3301 agatattgga caaatttaat gaagtattgg atctcctaga aataggaacc aaaatttcgg
     3361 acgacttgga caaagcaagg actatcgcta cggtgatggg ccccgttcgt acatcaatga
     3421 tccttttgca ggcttactta ctagatgaaa atagtagtaa tataaataca aaagaaattt
     3481 ttagtgatcc actgaatgca gccgtgacat tggctggtaa tgctttttgg agaggtattt
     3541 atgcggagga attggcgtat gatatggaaa attatgctct ccgtcatgat ccttgttatt
     3601 ggacgtataa ggctaagcct ccagatgctt ctggcaagat ttctgcagtc tttgactaca
     3661 aacaaactct accggctttc cttgaagcaa ttacggcacg tcttgctatg ctcgtcgcgc
     3721 tagaacctgg ctttagatca aatgaagctt atagaaggga actccttgat tattcagata
     3781 aattaattga ggttcataat cggattattc aaaatggaat tatcccttta aatggacctg
     3841 gtgaatatgc gttattttgt tcatacacag atgaaaaaac tcctagcggt aatgtgtgtt
     3901 atgtgatttc atggagaaaa aatggatgca aatatggtgt cgttgatctt tattctacca
     3961 caacaaaagt atcaaactat ccactgcctt tttttcttga gctcgaaaat cctccaccta
     4021 atttccaagc cctttcatcc gactatatca ttttaaataa tgatggaaat gtcctgattc
     4081 taattccaat aggaaaacta gacacctgcg atttggcgta ttggtttggg ttatatggtg
     4141 tatcggaatt atattgggat gcttgcgatt ctgtccgttt ttttttcgag gtcgtattta
     4201 agccaaaaat tattataagg actattaaac tttggcaaga tctctattca gaactaggac
     4261 tgccagaggt atggcgtacc atcaatcatt taaggtggct tgccggatac gatcctctag
     4321 aactgccaga tattccttgc acaacaattt cacttaagga atcttatttc atagaactat
     4381 ttaaactttt taaattttat caatgcattc gacgatctct gcgcaacata tatagcatct
     4441 ataatatagt aaagattcgg gctagtttca tattagccta taaatatcca gatccaacct
     4501 ccattaacgg aattttgaaa gctatttcgg tcagtatcag tatgagggaa attgcttcag
     4561 agctatatgt cttgcctcct atctcattaa gccgtctata tgaagattga acgaagaatc
     4621 tatttgtgaa acctaaattg ctggtccatg gattgtgata agagtaatta ccgagccctt
     4681 gctgtaggct tgaaagaaaa taaagttagg tgagcagggc agcgatccat atttcccgag
     4741 ggctcgcgca ccttcgtgat aggatgtcgc aaggttgctt ggattcgtga aaccgaaatt
     4801 atgggtcctt gaaccgtgat agcagtaatt caagagcatt tgcttcagtt gaaatagaaa
     4861 taaaaggcta ggtgagcagg gcagcgatcc atatttcccg agggctcgcg cacctgagta
     4921 cag
//
LOCUS       DAOOWY010000129         4919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_17280_length_4919_cov_14.158306, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000129 DAOOWY010000000
VERSION     DAOOWY010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17280_length_4919_cov_14.158306"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1159)
                     /locus_tag="PK105_08000"
     CDS             complement(56..1159)
                     /locus_tag="PK105_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU61696.1"
                     /translation="MPETYVISLIGCGRISSKHIEAALANKERVRLIACCDPILNRAQ
                     RCAQEYRAGLSSGGNIVVYADYHEMLAKEKPNICAIATESGYHPRIAIDCLEAGSHVI
                     CEKPMALSTRDADAMIDAARRHNRKLAVCFQNRFNAPVQRARAAIEAGRFGKMLHGMI
                     QVRWNRDEGYYKQAPWRGTWDLDGGTLMNQCTHGIDLLQWMMGEDAVRVRAVMRRFLR
                     PIEAEDFGAAIVEFASGAVGIIEGSADVFPKNLNETLSLFGEKGTVVIGGLAVNKIET
                     WRFADTQTVGDTEEEVLDPTAKDPPTVYGFGHTALYADFLDALEQDREPLVNGEAGKK
                     ALEIILAIYKSQKTGQPVTLPVEFSTSEMKGTF"
     gene            complement(1501..2700)
                     /locus_tag="PK105_08005"
     CDS             complement(1501..2700)
                     /locus_tag="PK105_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOU61697.1"
                     /translation="MADQNSSVPESISLIDLVATVWRQKWLVIIITAAAAVLSVLYSL
                     SKPNIYTATSTLLPISGSTSALSQYAGLAAMAGVSLPGASSADPTVKIQAILNSRELA
                     TKVINELDLIPKLIKEPEKLKTISPLSAALQTFQKNVFSVSVDTKTSVIKVSAKTKDP
                     ELSARIVNTAIDLLQQDLANRVLTASGKNIVVLEQQAAEQEKKVRELQNKMADYQKKN
                     KLIAPQTQSQQGLDLYRSLIQQKITLEIQISSLENTLSTDNPKISQARAQLAAIQKQI
                     EDFEKTGGGVGPAMTETPKALFEYANMQAQLELATKIYGSLLTSIENLRLQDATEKVF
                     VEVIDPAVPPELKSEPARATMCIVGTLAGGFASLLLAFARQALQKLIADPEVRAKFAK
                     KRETNSL"
     gene            complement(2734..>4919)
                     /locus_tag="PK105_08010"
     CDS             complement(2734..>4919)
                     /locus_tag="PK105_08010"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOU61698.1"
                     /translation="VVSGEYDTALKAHDRVRLFTTATLKVEPTKQGAIELQVSQTTTA
                     SIAPPLGDAGSLQVSPANANAPASAAGQAAPLAQATGPGGATPTAASTSIETDTGLYL
                     EVVYVSGTIRYQGPYARTPTLKLSSVVTTDQILQDTNLDYAELTRRKADGSWEYLTFS
                     PRDVLNKSYDLPLRALDSINFVPVKYLPDKIDFDKFSNAIAIMGTANFPGLYSFSESK
                     MISKVIGPEQLLSTTDIYYAEIERWKEGGRREYFTFSPIAVFQGRQDFQILPRDIIRF
                     LAAGDTGENHDFSRYPDTVLLKGSIRYPGRYAWYEGMQLSDIIQQQDLLIDTETSYAE
                     IRRYTETKDTIINFAPDQVVAKKGDVALSPRDTVMFYPKYYQKPVSIAGEVADPKIIP
                     YYDQMELSSILRSVTLTRAYSDLKAEIKKITGETTVVYLESYLKEQPTSKIILSPGDS
                     ITIRALLPDEHLPVVIVRGEVKQPQTLPFVEGMKLADALTAAGGYDANAYPKGLVLIR
                     KSVATAQQTQVDRLIAQLEAATAAGTSLPTSISSSQASDAASIVNMQVDLAMQKAKLG
                     NLKQLYKEGFGRISLDIPDSLEKLSTSPANIRLERDDLIYIPKIPTYVLVSGEVSSQN
                     VVLYRDGMTVRDAITESGWLSGEADLAKAYIIRASGKLESTQGKGFLFFKPNILNYKL
                     EPGDTVYVPMKSAKVSVAWAYVRDSFVLVSSILTSALTAKTLLGL"
BASE COUNT         1138 a         1226 c         1205 g         1350 t
ORIGIN      
        1 accgcagcct taacaaattg tggccgcgcc cccactacgc tcaatagaga gagcattaga
       61 aggttccttt catctcagac gtggagaact ccacaggtag cgtcactggc tggcccgttt
      121 tttggctctt gtagatcgca agaatgattt cgagcgcttt tttgcctgct tcgccgttta
      181 caagtggctc gcgatcctgt tcgagtgcgt cgagaaaatc ggcatagagc gcagtgtggc
      241 caaagccata cacagtggga gggtcctttg cagtaggatc gagcacttcc tcttcggtat
      301 cgcccacagt ctgtgtatca gcaaaacgcc aggtttcgat tttattgacc gcaagcccgc
      361 caatcactac tgtgcccttc tctccaaaga gcgagagtgt ctcattcagg tttttgggga
      421 atacatcggc gcttccttcg ataataccca ctgcgccgct tgcaaactcg acgatcgccg
      481 caccaaaatc ttctgcttcg atcgggcgca aaaatcgcct catcaccgca cggacccgta
      541 ccgcgtcttc gcccatcatc cactgcaaaa gatcgatgcc atgcgtacat tgattcatca
      601 gcgttccacc atcgagatcc caggtgccgc gccatggagc ttgcttgtag tatccttcat
      661 ccctgttcca tcgtacttga atcatgccat ggagcatctt cccaaaccga cccgcttcga
      721 tcgccgcccg cgcccgctgc actggcgcat tgaacctatt ctgaaaacat accgcgagtt
      781 ttcgattgtg gcgccgagcc gcatcgatca tcgcatctgc atcgcgcgtt gagagtgcca
      841 taggcttttc gcaaatgaca tggctccccg cctcaagaca atcgatcgcg attcgtgggt
      901 gatagcctga ctctgtggca atagcgcaga tattaggctt ttcttttgcc agcatctcgt
      961 gataatcggc gtatactact atgttgccgc cgctgctaag gcctgctcga tattcctgcg
     1021 cgcacctttg cgccctattc agaataggat cgcaacatgc aatgagccgc actcgctctt
     1081 tattagccag agcggcttcg atgtgtttag aactgatacg gccacaacca atcaatgaga
     1141 tgacatacgt ttcaggcatg attttccctt ttgtgaatat gctatctgta aactagtcgt
     1201 aaaactatta tagttatcct ctacaaaatt gctgttatac caaacataat attttataat
     1261 tttttggctt aataagtata tattcccctt ccctctctct atcgcggccg caacacgctg
     1321 atagtcaaga caaggtatgt ctttaaagca ttcatgatgc cttcaagggg catctgccgt
     1381 gcttcaaatt gaatgttcac aaaatgaaca ctcagtttat atacgaagta tcgtcaagat
     1441 gcaagggggt ctttaagaaa tttttaatga aatgaatgcg atacgacttg ataaatttcg
     1501 tcaaagggaa ttcgtttctc gcttttttgc gaatttagcg cggacttctg gatcagcgat
     1561 gagtttttgg agtgcctgtc gcgcaaaggc taaaaggagg ctcgcaaatc ctcccgcgag
     1621 cgtaccaaca atgcacatgg ttgcgcgggc gggctcgctc tttagctcag gagggaccgc
     1681 gggatcgatt acttcgacaa agaccttttc tgtagcgtct tgcagacgca gattctcaat
     1741 actggtcaaa aggcttccat agattttcgt cgcaagctct aactgcgctt gcatatttgc
     1801 atactcgaag agcgccttcg gcgtctctgt catggcaggc ccgacacctc ctcctgtctt
     1861 ttcaaaatct tcaatttgtt tttgtatggc ggcgagctgc gccctggcct gggaaatctt
     1921 tgggttatca gtagaaaggg tgttttcgag actcgaaatt tggatctcga gggtgatttt
     1981 ctgttggatg agcgagcgat acagatcgag cccttgctgg ctttgcgtct gcggtgcgat
     2041 gagtttgttc tttttttgat aatctgccat cttgttctga agttcgcgga ctttcttttc
     2101 ttgttcggca gcctgctgtt ccaatacgac aatgttctta ccgctcgcgg tgagcactcg
     2161 gtttgcaagg tcttgctgta ataagtcgat cgccgtgttg acgatgcggg cggagagctc
     2221 aggatctttt gtcttcgctg acacttttat caccgaggtc ttcgtatcga cggagacgga
     2281 gaaaacgttc ttctgaaatg tctgaagcgc tgcggagagc gggcttatcg tctttagttt
     2341 ctctggttct ttgataagtt ttgggatcaa atcgagctca ttgatcactt tggtggcaag
     2401 ctcacggcta ttgagaatcg cttgaatctt gacagtagga tcagcagagc tcgcgcctgg
     2461 aaggctcacg cccgccattg ccgcaagtcc cgcgtattgc gaaagcgccg aggtagagcc
     2521 tgaaattggc agtaaggtac ttgttgcagt atagatattc ggcttactca gtgagtagag
     2581 tactgaaagc actgctgcgg ctgcggtgat aatgatcacg agccatttct gccgccagac
     2641 agtggcaaca agatcgatca aactaataga ttccggtaca gaactatttt gatcagccac
     2701 acgtatctcc ttccatacta caccttactc atgttaaaga ccaagcaatg tcttagcagt
     2761 gagcgcgctt gtaagaatac tactcaccag cacaaagcta tcgcgcacat aggcccaggc
     2821 cactgagacc tttgcagatt tcatcggcac atacacagta tcgcctggct cgagtttata
     2881 attcaagata ttgggcttaa agaacaagaa acccttgcct tgggtgcttt cgagcttacc
     2941 ggatgcgcgg ataatgtatg cttttgcgag atccgcttcg cccgagagcc agcccgattc
     3001 agtaatcgca tcgcgaaccg tcatgccatc tctatagagc acgacatttt ggctcgatac
     3061 ttcgccggag acaagcacat aggtcggaat cttggggatg taaataagat catcgcgttc
     3121 gagccttata tttgctggcg atgtagaaag tttctcaagc gaatcgggta tgtcgagcga
     3181 gatgcgacca aagccttctt tatagagctg cttcagattg ccaagttttg ccttttgcat
     3241 tgccaaatca acttgcatgt tcacaattga tgcagcgtcg gatgcctggg agctgcttat
     3301 gcttgtcggc aaagaggttc ccgctgcggt ggctgcctca agctgagcga tgagcctgtc
     3361 tacttgagtc tgctgggctg tcgcgactga ttttcggatg agtacgaggc ctttgggata
     3421 ggcattggca tcatagcctc ctgccgcagt gagcgcatct gcgagcttca tgccttcgac
     3481 aaagggaagc gtctgtggct gcttgacctc gcctcgcact atgaccacgg gcagatgctc
     3541 atcaggcaga agcgctcgga tggtgatcga atcccctggt gagaggataa ttttgctcgt
     3601 gggctgctcc ttgagatagc tctcgagata caccaccgtt gtttcgcctg taattttctt
     3661 gatctctgct ttgaggtcgc tataggcacg ggtgagtgtg acgcttcgca gaatagatga
     3721 aagttccatt tggtcgtaat aggggatgat tttgggatcg gccacttctc ctgcgatgct
     3781 cactggcttc tgataatatt tgggatagaa catgacagta tcgcgcggac tcagggcaac
     3841 atcacccttc ttggcaacta cttggtcggg agcaaaattg ataatggtat cttttgtttc
     3901 tgtataccga cgaatctctg catagcttgt ctctgtatcg ataagtagat cttgctgctg
     3961 gattatatcg gagagctgca tcccctcata ccaggcgtag cgaccaggat agcgaatcga
     4021 gcctttgagg agcactgtat caggatacct cgaaaaatca tgattctccc ctgtatcgcc
     4081 ggcagcgaga aagcgaatga tatcccttgg aagtatctga aaatcctggc gcccttggaa
     4141 cactgcaatg ggactgaagg tgaagtattc tctccttccg ccctctttcc atcgctcaat
     4201 ttcagcataa tagatatcag tagtcgagag tagttgctca gggcctatga ccttggatat
     4261 catcttcgac tcgctaaatg aataaaggcc tggaaaatta gcggtcccca taattgcaat
     4321 tgcgttgctg aatttgtcga agtcaatttt atcaggcaaa tacttcacag gtacaaagtt
     4381 gatagaatct agcgctcgca gcggaagatc ataggatttg ttgagaacat cacgcggcga
     4441 gaaggtaagg tattcccagc ttccatcggc ttttcggcgt gtcaattctg cataatcgag
     4501 attggtgtct tggaggattt ggtcagtcgt cactaccgac gatagcttca atgtaggagt
     4561 ccgcgcatac ggcccttggt agcgaattgt gccagataca tatacaactt caaggtaaag
     4621 cccagtatcg gtctcaatgc tagtactcgc tgcagtaggc gttgcaccac ctggtcctgt
     4681 cgcttgagct agtggagcag cctgtccagc cgcgctagcc ggagcattcg cattagctgg
     4741 agatacttgt agagaccctg catctcctag tggtggcgca atgcttgccg tcgtggtttg
     4801 gcttacttgc agctctatag caccctgctt tgtaggctca acctttaaag tagctgtagt
     4861 gaatagccgc accctatcgt gcgccttaag cgccgtgtca tactccccac tcactactt
//
LOCUS       DAOOWY010000130         4908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_17345_length_4908_cov_6.315063, whole genome shotgun sequence.
ACCESSION   DAOOWY010000130 DAOOWY010000000
VERSION     DAOOWY010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17345_length_4908_cov_6.315063"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1213
                     /locus_tag="PK105_08015"
     CDS             167..1213
                     /locus_tag="PK105_08015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU61699.1"
                     /translation="MPEGLRRIKEREKRWYSRLAEGLASFFITVQVELKRLLTWGRTR
                     IVFAIIPQSERPPRKFEIGRFSLLIIVLTVVICVGAAIISIGAYGAKSIRVVSLEAQL
                     SDAYGTIDRMRESSENLISETTKFEEKLGQVIEIAQSRGKPALQRSASEEILKTAGLD
                     DPILGQFSDDPVSRDLLKLDSIAETLDKSLGALDDIAKILASQKDILTEVPNIWPIKG
                     NLGHISMYFGQNENPFSKGQWYLHTGIDISTNRIGDSILATADGKVIDIHYDSSLGNS
                     VTIQHSHGFTTRYGHLRSFAVKKGQRVFQGDVIGYLGNTGKTTGPHLHYEVYLGTSLI
                     DPLRFLNIRKEYHE"
     gene            1206..1544
                     /locus_tag="PK105_08020"
     CDS             1206..1544
                     /locus_tag="PK105_08020"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU61700.1"
                     /translation="MSRRDFSDCMIGEGTRVGGTIDSPGMVRIDGEFSGDIKSGASVI
                     ISKKAVVHAHINARQLVVAGKFSGSAVIEGGVRYAETARIEADCTGKFLIVEAGALVN
                     GQFARIDIED"
     gene            1616..2806
                     /gene="ricT"
                     /locus_tag="PK105_08025"
     CDS             1616..2806
                     /gene="ricT"
                     /locus_tag="PK105_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOU61701.1"
                     /translation="MSVEYNNHDIPEDILDMLGEEEFESPQVLKPIPKDIENLPQPIY
                     RLKTLHSSETFHAYYVSLDNNEHFMTICELEAALAGPRQEIHGVPPSEEAWNCKQGAV
                     SAAAEVLQPEAIQAMAIQGEGIEAEVDQAGIIQAEIIHPKSELIPNRALVIAPTKYGK
                     DLVMVLGVVHDFSMVNADELVAIERPATVDDYRRYLDNMEHEEEAFAKCKECIDSHNL
                     AMKLISAHCLLEDSKILFFFTAESRVDFRDLVKDLVGIFHARIELRQIGVRDEARVTG
                     GAGVCGRILCCHGISDKLNPVSIKMAKDQNLSLNSVKISGPCGRLLCCLSYEHQFYRD
                     ARRELPNEGVRFTYDGILFKVIEVNALLNKIRISGEDGRILDMDAARLKYLDSHWTII
                     EEES"
     gene            complement(2847..4616)
                     /gene="aspS"
                     /locus_tag="PK105_08030"
     CDS             complement(2847..4616)
                     /gene="aspS"
                     /locus_tag="PK105_08030"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOU61702.1"
                     /translation="MQFEQRTVTCGELRAAQIGKTVVLNGWVHRKRDHGGITFINIRD
                     RYGITQVVVDVDSAQELLDIANELKMEYCVSIRGIVRPRPDTMVNPSMPTGEIEIKAE
                     NIQILSRCTSLPFMIDEYTDAKEDMRLTYRYLDLRSSSMQRKIRLRHEAAFAVREFLV
                     GKGFYEIETPTFIRSTPEGARDYLVPSRLYPGKFYALPQSPQLYKQLLMISGFDKYFQ
                     LARCYRDEDSRGDRQPEFTQIDIEMSFVTRDDVLSLVEGMMGHLFKKTLGIELPKQFV
                     RLSYDDAMNRFGTDKPDLRFALELVDFVPFVDDHCSFQAFHSAIKEGGAVKALLVPNK
                     ADYSRRQLEELDAKTKVYKAHGLAWMKVGQSNRLEGGISKFFSEHSRIIEGLGASPGD
                     LILIVADGKKNIACSALGAVRSQLGKDLGLCDPDVFAFAWIIDFPLFEWNEEENRWEA
                     AHHMFTMPQTQYHETLEADPGSVKGDLYDLVLNGYEVASGSIRIHDPELQKRIFRIVG
                     LSDKEAQEKFGFLLEAFKYGPPPHGGIAPGFDRLVMVMAGETSIKEVIAFPKNSFAIS
                     PMDQCPSEVDERQLKELHIRIVD"
BASE COUNT         1293 a         1049 c         1254 g         1312 t
ORIGIN      
        1 attttatttg gctacaatcg cacaagtctg caaatattta tttttatgat tttataccag
       61 tgtctttgtg tcgattcgac ggctcactcg atcggctcga taaagtacta cattaattgc
      121 taaaaatttg cgataattta tcacttaggc aaggagaaag agcaaagtgc cagaaggctt
      181 gcgtagaatc aaagagagag aaaagcgttg gtacagccgt ctggcggagg gtctggcaag
      241 tttttttatt actgtacaag tagagctgaa gcgtttgctg acatggggaa gaactcgcat
      301 agtctttgct attatcccac agagcgaaag accccctcgt aagttcgaga ttggccgttt
      361 ttctcttttg ataattgtct tgactgtagt gatctgcgtt ggggctgcaa ttatttctat
      421 aggggcatat ggagcaaagt ccataagagt agtctctctg gaagctcagc tttccgatgc
      481 ctatggaact atcgatcgga tgcgtgaatc cagtgagaac ctgatctcgg agacaaccaa
      541 attcgaggag aagctcggac aggttataga gattgcacag agtagaggaa agccggcgct
      601 gcagcgcagt gcaagtgaag agatacttaa gaccgctggc ttagatgatc caattctggg
      661 gcagttttcg gatgatccgg tgagtcgaga ccttctcaaa ctcgacagca ttgcggagac
      721 attggacaaa agtctgggag cgctcgacga tatcgcaaag attcttgcta gccaaaaaga
      781 tatactgaca gaggtgccga acatctggcc aatcaagggg aatcttggac atataagcat
      841 gtattttgga cagaacgaga atcctttttc taagggacaa tggtatctgc acacgggtat
      901 cgatatttcg actaaccgaa tcggagattc tatccttgcc acggcagatg gaaaggtcat
      961 cgatatccat tatgattctt ctctaggtaa ttccgtcaca atccagcata gccacggctt
     1021 tacgactcga tatggtcacc tccgaagctt tgcggttaag aagggtcagc gggtctttca
     1081 aggcgatgta atcggatatc taggaaatac aggtaagaca acagggccac atctgcacta
     1141 cgaggtatat ctgggcacaa gtcttataga tcctctgcgt tttctcaata tacgcaagga
     1201 gtaccatgag tagacgtgac ttttctgact gtatgatagg agagggcacc agggtaggag
     1261 ggaccatcga ttctccaggc atggtgcgca tagatggaga attctcgggt gatataaaaa
     1321 gtggcgcttc tgtgataatc tcgaaaaaag ccgttgttca tgctcatata aatgctcggc
     1381 agcttgtggt tgcaggtaaa ttcagcggta gcgcagttat cgaaggtggg gtgcgttatg
     1441 cagagaccgc tagaatcgag gctgattgca cgggtaaatt tcttattgtg gaagcgggag
     1501 ctttagtaaa tgggcagttt gcgcgcatag atattgaaga ttaatgtcga gccgctgtta
     1561 ggaataaggt ccgcgaggac gcgatattag atccgcttta aatcaggagt aaatgatgtc
     1621 tgttgaatat aataatcatg atatcccaga agatatactc gatatgcttg gggaggaaga
     1681 gttcgaaagt cctcaggtat taaagcctat acctaaagac atcgagaacc tgcctcagcc
     1741 tatttaccgg ctaaagaccc tgcattcgag cgagactttt catgcttatt atgtatcttt
     1801 ggataataat gagcatttta tgaccatatg tgaactagag gctgccctgg ctggcccgcg
     1861 ccaggagatt catggagttc cgccttctga agaagcttgg aattgcaagc aaggcgcggt
     1921 cagtgcagct gcggaagtcc ttcaaccgga agccattcag gctatggcca ttcaaggaga
     1981 aggtattgag gcagaggttg atcaagcggg gattattcaa gcagagatta ttcatccaaa
     2041 gagcgagctc atccccaatc gagcgcttgt tattgcaccc actaagtatg gtaaggattt
     2101 ggtgatggtg cttggagttg tgcacgattt ttccatggta aacgcagatg aattggttgc
     2161 catagaaaga cctgctaccg tcgatgatta taggcgctac ttagataata tggagcatga
     2221 agaggaagcg tttgcaaaat gtaaagaatg tatagattca cataatctgg caatgaagct
     2281 tatctcggcg cattgtcttt tggaagacag caagatattg tttttcttta ctgccgaaag
     2341 tcgtgtggat tttcgcgatc ttgtcaagga tttggttgga attttccacg cccgcataga
     2401 actcaggcag attggggttc gagatgaggc tcgcgttaca ggaggtgccg gggtttgtgg
     2461 gcgaattctc tgctgccacg gcatttcaga caagctcaat cctgtatcga taaagatggc
     2521 aaaagatcag aatctttcgc tgaattcggt caagatctct ggtccatgtg gaaggctcct
     2581 ctgctgtctg agctatgagc atcaattcta ccgagatgcc cgtcgggagc taccgaacga
     2641 aggcgttcgc tttacttatg atggaatcct attcaaggtt atcgaggtca atgctcttct
     2701 aaacaagatt cggatctctg gagaggatgg tcggatcctg gatatggatg cggccaggct
     2761 aaagtacctc gatagccatt ggactatcat cgaagaagag agctaaagat agccaaggga
     2821 aagatgacta agcgaagata atattcctaa tccacaattc ttatgtgcag ctccttgagt
     2881 tggcgttcat cgacttcgct cgggcactga tccattgggc ttatcgcaaa tgaattcttg
     2941 gggaaagcga ttacctcttt tattgaggtt tcgccagcca tgaccataac tagcctatcg
     3001 aagccaggcg ctatgccacc atggggcggt ggtccatatt tgaaggcttc caacaaaaag
     3061 ccgaattttt cctgggcttc cttgtcgcta aggccaacta tgcggaagat ccgcttctgc
     3121 agttctgggt catgtatacg aatcgatcct gaggcaactt catagccatt cagaaccaag
     3181 tcatataggt cgccttttac cgatcctgga tcggcttcga gagtctcatg gtactgtgtc
     3241 tgtggcatgg tgaacatgtg atgggctgct tcccagcgat tttcttcttc gttccactcg
     3301 aagagaggaa aatcgattat ccaggcaaaa gcaaatacat cgggatcgca gagacccagg
     3361 tctttgccga gttggcttcg gactgctccg agagcgctgc aagcgatatt cttttttccg
     3421 tctgccacta tcaagatcag atcgccgggg ctagcgccaa gcccttcaat tatacgagaa
     3481 tgttcggaga agaatttgga tatgccaccc tcaagcctgt tactctggcc aaccttcatc
     3541 caggcaaggc catgagcctt gtacactttt gttttggcgt cgagttcttc cagctgccga
     3601 cgggaatagt cggccttatt tggaaccaat aaggccttga ctgcgccgcc ctcctttatg
     3661 gcactgtgga aagcctggaa gctgcaatgg tcatccacaa aaggaacaaa atctacaagc
     3721 tccaaggcaa aacggagatc tggtttatcg gttccaaagc gattcattgc atcgtcgtat
     3781 gataatcgca caaattgttt tgggagttcg attccgagcg tctttttgaa gagatgcccc
     3841 atcatgcctt ccacaaggct aagtacatca tcccttgtga caaagctcat ctcgatatct
     3901 atctgggtaa actcgggctg acggtccccg cgcgagtctt cgtctcgata gcagcgagcg
     3961 agctgaaaat acttatcgaa gccagatatc ataagtagct gcttataaag ctggggagac
     4021 tgaggaaggg catagaattt gcccggatac agccgcgatg gcacaagata gtctcgcgcg
     4081 ccttctggtg tagaacgaat gaaggtaggt gtctctattt cgtagaagcc cttgccaaca
     4141 aggaattcgc gcacggcaaa agccgcttca tgtcgcaggc gaatttttct ctgcattgaa
     4201 ctcgagcgaa ggtcgagata tcgataggta agacgcatgt cttccttggc atcggtatat
     4261 tcatcgatca taaaaggaag agaagtgcag cgcgagagaa tctgaatgtt ctctgctttt
     4321 atctctatct cgccggtggg catggaaggg tttaccatgg tgtccgggcg aggcctaact
     4381 ataccgcgga tcgatacgca gtattccatt ttaagttcat tggctatatc caagagctct
     4441 tgggcagagt ccacatctac tactacctgg gttatcccat agcgatcgcg aatgttgatg
     4501 aatgtgatgc caccatgatc gcgtttgcga tggacccatc cattgagcac tacagtcttt
     4561 ccgatttgag cggcgcgaag ctcgccacaa gtgaccgttc gctgttcgaa ttgcataatg
     4621 cgcctattct ataccggcgc atagacatgg gcaatactga ccatatataa gggtaagggt
     4681 ttggcgaatc tgtacgggaa tcgctttctc ttttgctatg gctaatttcc atgtataata
     4741 aatccgaacc attctttggt atggcaatct ttggattttt tattagagga ggtatacgat
     4801 gaagagattc tttgcattcg gattgattct gctcattttg gtgccggcca gctttgcggc
     4861 gccaaaatca aaagtcaaga tcgcgctgat tgtagaatcc acggtgga
//
LOCUS       DAOOWY010000131         4828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_17651_length_4828_cov_3.566520, whole genome shotgun sequence.
ACCESSION   DAOOWY010000131 DAOOWY010000000
VERSION     DAOOWY010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17651_length_4828_cov_3.566520"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..913)
                     /locus_tag="PK105_08035"
     CDS             complement(104..913)
                     /locus_tag="PK105_08035"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOU61703.1"
                     /translation="MSINIGIFGAGRFASAIAAEAMSMANLDRHVIDDEQFVISWMLH
                     RGEAVPPKPVDVVIDASNGSAVREHLDWALETETQLVIGATGWNIPDIEEKIGEKIGV
                     LISPNFSFAVAMMQRFAVDMAKFSDWYGEGDLIVFEHHHSGKKDAPSGTARSIASTII
                     GASRRYHAWSLSGSDPNILPITSLRAGSEVGIHELVFDAPHERFSIIHQARDRSLFAY
                     GALKGVRWILHRKGTYYMNDMLDDLINREGERDHNHDKRDRQFKRRHCNAV"
     gene            complement(910..2088)
                     /gene="asd"
                     /locus_tag="PK105_08040"
     CDS             complement(910..2088)
                     /gene="asd"
                     /locus_tag="PK105_08040"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOU61704.1"
                     /translation="MASSLQDSTPSQKRIPVTILGATGVVGQRFLRRIANHPWFYPAY
                     LTGSERSVGKRYSDACQWHLFSEAYAGLGDIIVVPSDPEHALAPIVFSALDAKAAREI
                     EPRFAAAGAYVFSNASAFRMEDDVPLIIPEINPEHIGLLQDQKNRRKWKGAIITNPNC
                     TTVMLASSLAPLHTVFGIDAVIMTSMQAISGAGYPGIPALDIMGNVIPYISNEESKVE
                     CESRKILGKLFSTPAGKTVVDSTFSVSATCTRVPIIDGHTLAISVKLRNTATLQDIQN
                     VFSVFVPKTQGYALPSAPRRFIRLSTKIDRPQPLLDVNIDEGMSIFIGRLRECPILDL
                     KFVAMGNNVERGAAGASVLNAEIALALGAIQVPSSYLQEEGRKIREGLLAGKETGGAA
                     "
     regulatory      complement(2203..2387)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(2476..3471)
                     /locus_tag="PK105_08045"
     CDS             complement(2476..3471)
                     /locus_tag="PK105_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61705.1"
                     /translation="MPLIIQKYGGTSLIEASSREIVAKKVTAARAQGEKVVLVISAIG
                     RRGYPYATDTLLDLLTQYEGGADPLTSDLIASCGEIISCCVISSLLNSKGIMAIPMSA
                     YSAGIEVEGPFGDATPVRIDSSRINRILDDGIVPVIAGFQGIDNYGRIYTLGRGGSDT
                     TAVALGIALHADYVDIYKDVPGVAKADPHVILNAQFMSFLDYDSMFRLANHGARVLHD
                     KSAILAKDSGIKIRIRSTFDDSKGTLIAEPEDGYKVPHFIGLTTNTMSDGRLKVTAVM
                     ARGESREEEAKIAWLIETSGFSAVSIDCDDQDAFAFACDPSDGKRFAIALYSLLS"
     gene            complement(3475..4494)
                     /locus_tag="PK105_08050"
     CDS             complement(3475..4494)
                     /locus_tag="PK105_08050"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOU61706.1"
                     /translation="MKSFRVAVVGALGAVGTEMIKTLERRMFPISAMIPIDVPEFAGQ
                     KIMAGSREVVVRGASKGAFKDVDIALFSAGAEASLELAPIAVSEGAVVIDNSSAWRMD
                     PDVPLVVPEVNPEALDGHKGLIANPNCSTIQMVVALKPIHDAYRIKRVVVSTYQAVSG
                     GGTSAINELREQVAQFVRGEPITVKVFPRQILFNAIPHIDVFMENGYTKEEMKMVNET
                     HKILDPNIEVSPTAVRVAVFRGHSESVNIETEKAFSLSEARAMLETAPGIKVMDDTTE
                     LMYPTALDADGQDAVYVGRLRKDPTVAHGLNMWVVSDNLLKGAALNAVQIAETLLKRG
                     FKGRT"
     gene            complement(4481..>4828)
                     /locus_tag="PK105_08055"
     CDS             complement(4481..>4828)
                     /locus_tag="PK105_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU61707.1"
                     /translation="NAISVRLPITNLPVIDREKCTACGLCIPACPGLAITIKSIQGDK
                     ARIRFPWEYLPMPNRGERVAMVDRMGEVRCEGLIISTQNIARNNRTAVVTAEFSKEFV
                     QEIVSMRRLHEEL"
BASE COUNT         1269 a         1276 c          937 g         1346 t
ORIGIN      
        1 tatgctgcca ttttccaata attcgagaaa tagagcccac tgttccttat cgaatccagc
       61 tcgctcggaa aaaacatctt cgagtgttcg agctcttgca tcttcatacg gcattgcaat
      121 ggcgacgctt aaactgtcga tctcttttgt catgattatg gtccctctca ccttctctgt
      181 ttatcaaatc atctagcata tcgttcatat agtaggttcc ctttcgatgc aaaatccatc
      241 taactccctt caaggcacca taagcaaaga gactcctatc tcttgcttga tgtattatgg
      301 agaagcgctc gtgcggcgca tcgaatacca attcgtgaat gcctacttcg ctgcctgctc
      361 taagagatgt tattggcaga atatttggat cagatcccga taaagaccat gcatgatatc
      421 ttcttgaagc acctattata gtgctggcaa tcgatcttgc cgttccgctg ggtgcgtctt
      481 tcttgcccga atggtgatgc tcgaaaacaa tcaaatctcc ctcaccatac caatcagaaa
      541 attttgccat atctaccgca aaacgctgca tcattgcaac tgcaaaggaa aaattaggac
      601 ttatgagaac gccgattttt tcgccgatct tctcttctat atctggtata ttccaaccgg
      661 tcgcgccaat cacaagctga gtctcggtct caagcgccca atccaaatgt tccctaacag
      721 cggaaccatt gcttgcatct attaccacgt ctactggttt tggaggaacc gcttcacctc
      781 tatggagcat ccaagatatc acaaattgct catcatctat gacatgccta tccagattcg
      841 ccatcgacat agcttctgcg gcaatggcac ttgcgaatct tcctgcacca aaaattccaa
      901 tattgatact catgccgctc ctcccgtttc ttttcccgct aagagacctt ctcttatttt
      961 tctcccttct tcctgaagat acgaagacgg aacttgaatt gctcccaatg ctaaagcgat
     1021 ctctgcattc aataccgatg cgccagctgc ccctcgctca acattgtttc ccatagctac
     1081 gaattttaaa tcgaggattg gacattcacg caaacgccct ataaagatgc tcattccttc
     1141 atctatattc acatccagaa gcggctgagg tctgtcaatt ttggttgaaa gccgaataaa
     1201 gcgccgagga gcgcttggaa gagcatatcc ttgtgttttg gggacgaaaa cagaaaagac
     1261 gttctgaata tcctggagcg ttgctgtatt tctaagcttt accgatatgg caagcgtatg
     1321 gccatctatg ataggaactc ttgtgcaagt ggcagaaacc gaaaaagtgc tgtcaacaac
     1381 tgtttttcct gctggggtcg aaaataattt gccaagaatt tttcttgatt cgcattctac
     1441 ctttgattct tcattggaga tgtatggaat gacattgccc ataatatcta gggcaggtat
     1501 gccaggatat ccggcaccgc tgatcgcttg catgcttgtc atgattacgg catcgattcc
     1561 aaaaactgta tggagtggag caagcgatga ggcaagcatt acggtcgtgc agttcggatt
     1621 tgtgataatg gcgcctttcc attttctccg attcttctga tcttgaagca accctatatg
     1681 ctctgggttt atctctggaa tgatgagagg cacatcatct tccattctaa atgcactggc
     1741 atttgaaaaa acataggctc ccgcagctgc aaagcgcggc tcgatctcgc gtgccgcttt
     1801 tgcatctagg gcggaaaaga caattggcgc taatgcatgt tcaggatctg acgggacaac
     1861 aataatgtct ccgagtccgg cataggcctc gctgaaaaga tgccattggc aggcgtcaga
     1921 atatcttttg cctaccgatc gctcgcttcc tgtcagatat gctgggtaga accaggggtg
     1981 gttggcaatt ctcctaagaa aacgctgacc gacaacacct gttgctccca agatcgtaac
     2041 aggaattctc ttctgcgagg gggttgaatc ttgtaggctg ctagccatgt aacctcctcg
     2101 ccgaaggagg tctacgctag cctttattat ttgctgaaaa atgattgcaa aagacgaccg
     2161 aggtactgca atacctatct ttttacaatc aaatagcaga aggctcacgt caggcgctcc
     2221 gcgggaaggt gcttccgcgc gacagccgcc gggtattaac ccagacgtcc aagaacagca
     2281 ccgagagatg gtaactgttc cttcggcgat tctccccttt ccgaaacttt cattggctct
     2341 ctccgcctcc agtccgtact gatgggaatc gctcctccat cggtgaatgg gactctatca
     2401 tagaagcata aaacaatgca ataggttcta taaagaattc cagaaattat tgagctcttc
     2461 ctgcaatctt tcacatcatg acaaaagcga atagagagct atcgcaaatc tctttccatc
     2521 tgaaggatcg caagcaaaag caaatgcgtc ttgatcatca caatcgatgg agactgcaga
     2581 gaatccgctc gtttcgatca accaagctat tttggcctct tcctctctac tctctcctct
     2641 cgccattaca gcggtaacct tgagccttcc atcggacatt gtatttgttg tcaatcctat
     2701 aaaatgtggt accttatagc catcctctgg ctccgctata agagtccctt tcgaatcgtc
     2761 aaaagtagaa cgtattcgaa tcttgatgcc agaatctttg gccaaaatag cagacttgtc
     2821 atgaagcacg cgcgctccat gatttgccaa tcgaaacatt gaatcataat caagaaaaga
     2881 cataaattgg gcatttagga taacatgtgg atctgctttt gcgacaccag gtacatcttt
     2941 gtagatatct acataatcag catggagcgc gattccaaga gcgactgcgg tggtgtcgct
     3001 gcccccacgc ccgagagtat agatacgacc ataattatca atcccttgaa agcctgctat
     3061 aaccggaaca atgccatcat ctaatatcct attgatacgc gaagagtcaa ttcgcacagg
     3121 tgttgcatca ccaaaaggtc cttcaacttc aataccagcg ctatatgcag acattggaat
     3181 agccattatc ccctttgaat tcaataggga agaaataaca cagcatgaga tgatctctcc
     3241 gcatgaagca atcagatcag aggtaagcgg atctgctccg ccctcgtatt gtgtaagcag
     3301 gtcgagcaag gtatcagttg catagggata tccccgacgc cctattgcag aaataaccaa
     3361 tacaactttc tctccctgag ctcgagctgc ggttactttt ttcgctacga tctctctaga
     3421 agaagcttct attagcgaag tcccgccata cttttgaatt atcagtggca ttctttaggt
     3481 tcttcccttg aatcctctct taaggagagt ctcagcaatt tgtaccgcat tcaacgctgc
     3541 acccttcaag agattgtctg atacgaccca catattcaaa ccatgcgcaa ctgtcggatc
     3601 cttccgaaga cgccctacat acaccgcatc ttgcccatct gcatcgagcg ctgtgggata
     3661 cattagttca gtggtatcat ccataacctt tatcccaggt gcagtttcta gcatcgccct
     3721 cgcctcagaa agagaaaatg ctttctctgt ctctatgttc actgattccg aatgtcctct
     3781 aaataccgca acccgcactg ctgtaggact cacttctatg ttcggatcta atatcttatg
     3841 cgtctcattg accatcttca tttcttcctt cgtataccca ttctccataa acacatcgat
     3901 gtgcggaatc gcattaaaca aaatctgtcg tggaaacacc ttcaccgtta ttggctctcc
     3961 cctcacaaac tgcgccacct gttctctaag ctcattgatt gcactcgtcc ctcctcccga
     4021 taccgcttga taggttgata ccactaccct ttttatacga taggcatcat gaataggctt
     4081 caaagccact accatttgaa tcgttgaaca gttgggattc gcaattaatc ctttatgccc
     4141 atccaaagcc tctggattta cctcaggcac caccaacggc acatcaggat ccattcgcca
     4201 tgcactcgaa ttgtcaatca ccactgcacc ttcagataca gcaatcggtg ctagctcaag
     4261 acttgcctct gcacctgcag agaacaatgc gatgtccaca tccttaaatg cacccttgct
     4321 tgctccacgc actaccactt ctcgactccc tgccataatc ttttgccctg caaattccgg
     4381 tacatctata ggaatcattg cagatatcgg aaacattcgc cgttctagag tcttgatcat
     4441 ctctgtacct accgccccca acgcacctac tactgccact ctaaagctct tcatgtaacc
     4501 tcctcatgct cactatttct tgcacaaatt ccttagaaaa ttcagccgtt acaaccgcgg
     4561 ttctattatt ccgagcaata ttttgtgtcg aaattatcag gccttcgcaa cgaacttctc
     4621 ccattctatc caccatcgcc accctctctc ctcgattggg cataggcaag tattcccatg
     4681 gaaagcgaat acgtgcctta tcaccttgaa tgctctttat tgtaatagcg aggccaggac
     4741 atgcagggat acataatcca catgctgtac atttctctct atcaatgact ggaaggttag
     4801 taataggaag cctcacactt atagcatt
//
LOCUS       DAOOWY010000132         4556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_19058_length_4556_cov_8.411908, whole genome shotgun sequence.
ACCESSION   DAOOWY010000132 DAOOWY010000000
VERSION     DAOOWY010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19058_length_4556_cov_8.411908"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PK105_08060"
     CDS             complement(<1..1171)
                     /locus_tag="PK105_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU61708.1"
                     /translation="MRDIFDVAIIGAGVCGANIARKLSQYELKVALLEKEIDVSLGTS
                     KANSGIVHGGFHDHISTLKARLELSGALMFDRLHEELDFPFERCGILVAALHEDEVRV
                     IEQLYLQGVENGVIGIEMCSRERMLELEPKLNPEVVGGLYAPSGGILEPYRFVFSLVE
                     SAEKNGVEIKTQFEVISAVREGDCWRIIAADGREIEARFVVNSAGLYADTISAAFGGE
                     RFEIVPRKGEYYLLDRTTKAKPSRVIFPVPTEVSKGILVIPTVEGTVLIGPTATSARD
                     KEDFATSREQLEHILRTARMMVPSISENDVITSFAGLRASYGNDFYIALSEKTPALVQ
                     VAGIQSPGLTASPAIGEYVKDLLKKAGLRLIEKPSWDPYIRKIPRARDADPFALDS"
     gene            complement(1261..2865)
                     /locus_tag="PK105_08065"
     CDS             complement(1261..2865)
                     /locus_tag="PK105_08065"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOU61709.1"
                     /translation="MKKFVVLLMIALFVFSAQSSFACTIVGVGADATVDRSTIVTHND
                     DSTSADFRLWIIPGQEWPEGSTRDLVIDSHNYGDFGKYPEVKDYGNGMLVSEIPQASE
                     TYAYFHSRYSFINEKGVAMGESTFNFDQSTDLRKKTYKLIFGNNTGLIDCWNAQDIAL
                     ERASTAREAIQIMGDLVEKYGWKDPGETMDVADGKEVWIAEFYGLDLWAAVRIPSNAF
                     FVAANRARIDHIDFNDHENYMYSPNIKSFAIENGLWSEDSGVPFSPAEIYAPYEGLYS
                     TRREWRALSLVAPSLNLDPDAKRFPLWVIPEKKLSVQDVLEICGDYYQGTPYDLSLAP
                     EAGPYGDALNPYHKERSINLFRTCYVMIANIKDRLPDPVKCLVWYGYGAADTTYITPL
                     WPTMKELPEFYRTGSRFEEFRRDSGWWTNSYVQQTARSNYQSAVKEIHNFRNPRLATL
                     YTVTEEIQNTAASLIKDGKEKEAIDLVSSFAYTTAVKWHDDWLKFGDELYGTYMWGYK
                     DMKSQPPSAWWNEIMQKAPRNPVPFK"
     gene            complement(3013..4200)
                     /locus_tag="PK105_08070"
     CDS             complement(3013..4200)
                     /locus_tag="PK105_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HOU61710.1"
                     /translation="MKKNNSMGEKQSFPYFKVICLVIALIFAFFGGREFYELRHFKET
                     FVSAPGLTEVKMLSDYLSSLKGTWMDTPIFVYDSGVPGGNILYMANVHPYEPATSLSA
                     YILMENINVTKGKVFVIPQGNRSGSTTGMLGNAYPKFFHVPTSFGEKTLRIGDRGTNP
                     LDQWPDPFTFVHYPSRQNLAYQDIRNMNRTYPGRENGTLTERASYAIMELIRKENIDL
                     SIDVHEASIMYPVVATYVAHERSFDIAMMAAMMLTATSFDMKCESSPKGLRGLSHREW
                     GDFSDTLAVLMETPEPFIDRVVGPMTEELFTEGKDEFLATAAEHGLTYVPYNLEFGSP
                     LWYRCGRHLSGALEVINQMNQFYPEKEVIVEWPTYEDLKEHDCGYFFHDPDKADPNFV
                     FEI"
BASE COUNT         1237 a         1116 c          983 g         1220 t
ORIGIN      
        1 gagaatctaa tgcgaaagga tcggcatcgc gggcgcgggg gattttgcga atgtacgggt
       61 cccaggaggg tttttcgatg agcctgaggc ctgctttttt taggagatcc ttgacatatt
      121 cgcctatggc ggggcttgcc gtaaggccgg gcgattggat acccgctact tgcactaggg
      181 cgggggtctt ttcggatagc gcaatataga aatcattgcc ataggaagcc ctgagccctg
      241 caaaactggt aataacatcg ttctctgaaa tcgaaggaac catcatgcga gccgtgcgca
      301 gtatatgctc gagctgctcg cggctcgttg caaaatcctc cttgtctcgg gctgaagtgg
      361 ctgtaggacc aataagcacg gtgccttcca cggtgggaat aaccagaatg cccttggaga
      421 cttcggttgg aacaggaaaa atgactcgtg agggtttggc tttcgtggtg cgatcgagaa
      481 gataatactc tcccttgcgc ggtacaattt caaaacgctc accgccgaat gctgcggaaa
      541 ttgtatcggc gtatagacct gcagagttca caacaaagcg agcttctatc tcccgtccat
      601 ccgcggctat gatgcgccag cagtctccct cacgaactgc cgagataacc tcgaactgtg
      661 tcttgatctc taccccattc ttctccgcgg attccaccag cgaaaagaca aaacgatagg
      721 gctctaaaat accaccagaa ggcgcataga ggccgccgac cacttcgggg tttagctttg
      781 gctctagctc gagcatgcgt tctcgagagc acatctcgat tccaataaca ccattctcta
      841 caccctgaag atacagctgt tctatgactc gcacctcatc ctcatgaagc gcggcaacca
      901 gaatcccaca gcgctcgaaa ggaaaatcta gctcttcgtg taggcggtcg aacatcagag
      961 ctccactgag ttctaaacga gcctttagag tacttatgtg atcatgaaaa ccaccatgca
     1021 caataccgga atttgccttg gaagttccaa gcgatacatc gatctctttt tccagcaaag
     1081 ctacttttag ctcatactgc gataacttac gcgcaatgtt agctccgcac acgcctgcgc
     1141 caattatcgc aacatcgaat atatctcgca tttatagttc cctaatagaa acatgaggac
     1201 agctctattt gctgtcctca tgttcatgat tatttacaaa atataagtct tattaggcta
     1261 tcacttgaat ggaacagggt ttctgggagc cttttgcatt atctcgttcc accaggcgct
     1321 cgggggctga gacttcatat ccttatatcc ccacatgtag gttccataca gctcatcgcc
     1381 aaacttgagc cagtcatcat gccatttcac tgcggtagta taagcaaagg aagatacaag
     1441 atcgatcgct tccttctctt ttccgtcctt gatcaaactc gcggccgtat tctgtatttc
     1501 ttcggtaacc gtataaagag tagcaagtct tgggttacgg aaattgtgaa tttcctttac
     1561 cgcgctttgg tagttgcttc tggctgtctg ctggacatat gagtttgtcc accaacctga
     1621 atctcgacgg aattcttcaa aacggcttcc agttctatag aactcaggca attctttcat
     1681 ggttggccag agcggcgtta tataggttgt atcggcagca ccatatccat accacacaag
     1741 acatttcaca gggtcaggta accgatcttt tatatttgcg atcattacat agcaggtcct
     1801 aaagagattg atcgatctct ccttatggta ggggttaagg gcatcgccat aaggaccagc
     1861 ctcaggagcc agagaaagat cataaggtgt cccctgataa tagtcaccgc aaatctctaa
     1921 tacatcttgc acagaaagct ttttttccgg aatgacccag aggggaaaac gctttgcatc
     1981 aggatcgaga tttagagagg gcgctacaag gcttagagct ctccactcgc gtcgggtact
     2041 atagagcccc tcataaggcg catatatctc ggctggtgag aagggaactc cggaatcttc
     2101 agaccagaga ccgttctcta tagcaaaact ctttatgttc ggagaataca tatagttctc
     2161 atgatcatta aaatctatat gatctatgcg tgccctgttc gccgcaacaa agaacgcatt
     2221 ggatggaatg cggaccgctg cccaaaggtc cagcccgtag aattcagcaa tccatacttc
     2281 tttgccatca gccacatcca ttgtctcgcc gggatctttc catccgtatt tttccaccaa
     2341 atctcccatg atttgaatgg cttctcgagc tgtggatgct cgctctagag cgatatcttg
     2401 agcattccaa caatcgataa gacctgtgtt gtttccaaag atcagcttat aggtcttctt
     2461 gcggagatcc gtcgattgat cgaaattgaa tgtgctctcg cccattgcca cgcctttctc
     2521 gttgataaag gaataacgag aatggaaata ggcataggtc tcagaagctt gaggaatctc
     2581 ggagactagc atgccatttc cataatcctt cacttcggga tatttgccaa aatctccata
     2641 gttatgcgag tcgatgacaa gatcgcgagt cgacccttcc ggccattctt gccctggaat
     2701 tatccaaagg cgaaaatctg ccgatgtaga atcgtcgttg tgagttacta ttgtcgaacg
     2761 atctacagtt gcatctgcgc caacgccgac aatagtgcat gcaaacgatg actgcgcact
     2821 aaatacgaat agggctatca tcagcagcac tacaaatttc ttcatatcat actcctttct
     2881 tggggaattc cccatattct cttgtctccg cttatctttt gataagttat cctatctcag
     2941 atagccgcgg tatagagcca aattccaaat atcttcgaca ccatgtcatt cttctattct
     3001 ttttattttc gcttatatct cgaatacgaa attgggatcc gctttatcag gatcgtgaaa
     3061 gaagtaccca caatcatgct cttttagatc ttcgtaagtc ggccactcaa cgatgacttc
     3121 cttttccgga tagaactgat tcatctgatt gatcacttca agagctcctg aaaggtgtct
     3181 tccacagcga taccaaagag gagaaccaaa ctcgaggtta tatggaacat aagtgagtcc
     3241 atgctccgct gcggtagcta agaattcatc cttcccttca gtgaacagct cctctgtcat
     3301 aggccccact actcgatcga taaaaggctc cggagtctcc atgagcacgg caagagtatc
     3361 gctgaaatcg ccccattcgc gatgagacaa tccacgtaga ccttttggag aagactcgca
     3421 tttcatatca aaactggtag ccgtaagcat cattgctgcc atcattgcaa tgtcaaacga
     3481 acgctcgtgt gctacatagg tggctaccac aggatacata atagatgctt cgtgaacgtc
     3541 tatagatagg tcgatatttt ccttacggat aagctccatt attgcatagg aggctcgctc
     3601 cgtgagagta ccattttccc ttcctggata ggtccgattc atattgcgaa tatcctgata
     3661 ggccaaattc tgtctcgatg ggtaatggac aaatgtaaaa ggatcaggcc attgatcgag
     3721 aggattcgtt ccacgatctc cgattcgcag cgttttctct ccgaaagatg tcggaacatg
     3781 aaagaactta ggataagcgt tacccagcat gcctgtagtc gatccacttc tgttgccctg
     3841 aggtattacg aaaaccttcc ccttggttac attgatattc tccatcaaaa tataagccga
     3901 aaggctggta gccggctcat aaggatggac attggccatg taaagaatat tgcctccagg
     3961 tacacctgaa tcgtagacaa atatcggagt atccatccac gttcctttga gactcgaaag
     4021 gtaatcgctc agcatcttta cctcggtcaa accaggagcc gagacaaagg tctctttaaa
     4081 gtgcctgagt tcgtagaatt cccttcctcc gaagaaagca aatatcagtg cgattacaag
     4141 gcatatcacc ttaaaatatg gaaaagattg cttctccccc atgctattat tctttttcat
     4201 aattccatcc tctcatttga tgcccagcac gcgcagaaga aagggaataa tgcagtaggc
     4261 atatacgatg tcatacactc ggcctgtacg tgatgtcctc ttgaataaat tcctaaggag
     4321 gaaaaagcct ataaaagcag caaaaatgaa atagattata tgtattactt gttcaagaaa
     4381 aaccatttgt ttctcctcat ttaaggaaac tgagcttgtt aggaataatc agcatgataa
     4441 gtgccaaacc tccaagcacc aataaaggca gcaatatctg ttttaggaaa gacatataag
     4501 agcctttata acccactgtc tcgatggaga cacgacctac aattctcgag ggagga
//
LOCUS       DAOOWY010000133         4513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_19305_length_4513_cov_13.186182, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000133 DAOOWY010000000
VERSION     DAOOWY010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19305_length_4513_cov_13.186182"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1104)
                     /locus_tag="PK105_08075"
     CDS             complement(100..1104)
                     /locus_tag="PK105_08075"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOU61711.1"
                     /translation="MKKLKKSTASWNKVLPMSPEKSIIAIMILFVTLSSLPSQDIFTQ
                     SAGPGAQEIPGLIPIATFDEGRSYRTVVGSYPILSLKGSWREMGRQYGALLSKELKEF
                     YAAINADLQARGLGQEHIEGVQATFQTYMPEMKQLIEGMSETSGLSFEEHIMLDASFY
                     ILPDLVIEAAQAMASCSGIAVSSPRTADGKLYFARNWDMTQAAMQPYLRYMALVVFNP
                     TDGGLSFANVRPIGQVYVETGINEKGVFVELNNGSASDPNINEGAVFSVASIFDFLRT
                     SETLDEMIQNIVTTKMDASYIIQAASSERAVSVEKPTFDARVIEQQNGALYALNNFAI
                     "
     gene            complement(1242..1889)
                     /locus_tag="PK105_08080"
     CDS             complement(1242..1889)
                     /locus_tag="PK105_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOU61712.1"
                     /translation="MSVITFSREPCSNGTLIAQSVAEKLGYRLATKKLIEEVLLQYGF
                     VDVEEAYDSMPNFWTRFDDRTHQIVIMFDRVIKALAKLGNVVIIGRGAFRVLAPYRDV
                     LNVRIKSPFPYRVATFMSRYNVLDQHDAEVAILEADRIRSAFLEVHYGIKWDSMTNFD
                     LVLDTSKIPPHTAIPMIIKANKAVLSTVCEPRESASSIDVDDILLEATRRVIENN"
     gene            2423..2695
                     /locus_tag="PK105_08085"
     CDS             2423..2695
                     /locus_tag="PK105_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HOU61713.1"
                     /translation="MESKVLGTGQLRFIEVKGRVEGQDYITVTKNEIMHSLNKPDSYI
                     LAIVKFKADGSHTLHYITQPFAKEPDIGKRTRNYDIQMMLEKGTRQ"
     gene            2994..3911
                     /locus_tag="PK105_08090"
     CDS             2994..3911
                     /locus_tag="PK105_08090"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HOU61714.1"
                     /translation="MEYKKPPLNYEAQAKLLLKRGLVADEAILIKHLKSVNYYRLSGY
                     LYTFRIIDDNGNHLDSYYPDTCFDTVWQRYTFDRQLRLIILDGIERIEIALKTKLTQV
                     FSMKYGAFGYLDIKNLPRFSQDRHSIFITKLREETQRSNEDFVQHFFKKYGDYHNDLP
                     LWMATEIMSFGSLLTLLNGMAEQDLKVIAREFNLNVPILDSWILTLSSIRNICAHHGR
                     LWNRELGIKPKIPSGSKYAEWHSPIETGNKRIFGVLTIIRYMLRYIAPQSAWPQRFMN
                     LLSQYPEIPLNFMGFPKNWETHAIWKYHE"
     gene            4116..4274
                     /locus_tag="PK105_08095"
     CDS             4116..4274
                     /locus_tag="PK105_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61715.1"
                     /translation="MIQELATLYRVHPNLVALWKKQLQENAPELFERTQKDKKKEVAQ
                     HKEEELYK"
BASE COUNT         1371 a          940 c          984 g         1218 t
ORIGIN      
        1 cgaaagaacc gtgtgtacca tagaacccca tcactgtcgc tttaggaagg cgaccaattg
       61 ccatcgcaat acggcgggag tcgcttcatt ctattcctct tatattgcaa aattgttgag
      121 cgcatacaag gcaccatttt gctgctcgat aacgcgagca tcgaaggtag gcttttccac
      181 agagacagcg cgttcagagc ttgcagcttg gataatgtat gacgcatcca tttttgtcgt
      241 cacaatgttc tggatcattt cgtcgagcgt ttcgcttgtg cgaaggaaat cgaaaataga
      301 agccaccgaa aataccgcac cctcattgat atttgggtct gaagccgaac cattgttgag
      361 ttcaacaaac acgccctttt catttattcc tgtttcgacg tatacctgcc ctattggtct
      421 cacattagcg aaggacaatc ctccatcagt gggattgaaa actacaagcg ccatatagcg
      481 aagatacggt tgcatagctg cttgggtcat gtcccagtta cgcgcaaaat agagcttccc
      541 gtcggctgtc cgagggctag aaacggcgat tcccgaacac gaagccatag cttgtgccgc
      601 ttctatgacc aagtctggca aaatataaaa agaggcgtcc agcattatgt gctcttcaaa
      661 agataaaccc gaagtctcag acattccttc tataagctgc ttcatttctg gcatgtaggt
      721 ttggaatgta gcttgtacgc cttcgatatg ttcttgtccc aggccacgag cctgcagatc
      781 ggcattgatc gccgcataga attctttgag ctcctttgaa agcagtgctc catattgtcg
      841 acccatttcg cgccaacttc ccttgaggct taaaatagga taagagccaa ccacggttct
      901 atagcttctt ccttcatcaa atgtcgctat aggaattagc ccagggattt cttgagcccc
      961 aggtcccgcc gactgagtga agatatcttg tgaaggtaag gagctcaagg taacgaacaa
     1021 aatcatgatt gctataatgc tcttttcggg ggacattggt agtactttgt tccatgaagc
     1081 cgttgatttt ttcagttttt tcattgtttt ctccttagaa gatcctagaa tactctttaa
     1141 tatgccgatc aattggaaga aggatacatt aaaaactata gcatgtcttt tgcatcaaag
     1201 aaatattcac tgacaaaata aaaagatcgg ccttttgcct tttaattatt ttcgatcact
     1261 ctgcgcgttg cctcgaggag aatgtcgtct acatcgatag aagatgcgga ttctctgggt
     1321 tcgcagactg tgctcaatac ggccttgttg gctttaatga tcattggaat cgcggtgtgc
     1381 ggcgggattt tcgatgtatc gaggacgaga tcgaaattgg tcattgaatc ccactttatt
     1441 ccatagtgaa cttcgagaaa ggccgatctt atcctgtccg cttcgagaat cgccacctcg
     1501 gcatcgtgct gatccaagac attgtaccgg ctcatgaagg tggctaccct atatggaaat
     1561 gggcttttta ttcgcacatt cagcacatcg cggtaaggag ccagcactct aaatgcacct
     1621 ctgcctatga tcactacatt gccaagcttg gcaagtgctt ttattacacg atcgaacata
     1681 atgacgatct gatgagtcct atcgtcgaag cgcgtccaga agttgggcat gctgtcgtaa
     1741 gcttcttcta catccacaaa cccatattgg agcaatactt cttcgatcaa tttctttgtg
     1801 gcgagacgat aaccgagctt ttcggcgaca gattgagcaa taagagtacc gttggaacac
     1861 ggttccctag aaaaagttat aacactcatg gcagacctcc ttctctaatt atagtattgg
     1921 ctaaaagata ggtgcagtca atgaaagata tatacagaaa atgacgataa taaagtcatg
     1981 tgggacactc tgtgtccctc gccaggagta taatcagaaa taaaaaaata gaaggcggag
     2041 gcctccatga gccaaaccga gagaattttc tacatcgatc gcatgataag ggagcatgga
     2101 ggcgtctccg cccgccaaat atgcgataaa ttcgaggtat cttcgcgcca agccaagcgc
     2161 gatataggaa aagaggcttt tgcttgcaat attaaggata aaaggtgcac aggttcacgg
     2221 tattgttcat aattctatcc cggaggacat gaaagctgaa tttctgaaaa catttttacc
     2281 tataaggcgg atattacaaa agaggagcaa ctaaaagaag tagtacaagc aatggctcat
     2341 gcaacgtcta ctttagatat tcttatcaat gatgcagcgg ttcactttga aaatcatcat
     2401 ctgatctgaa gtagggtggg atattgagag caaggttctc ggcacgggtc aattacgctt
     2461 tatcgaagta aaaggaagag tggaaggcca agattatatc accgtcacca agaacgaaat
     2521 catgcactcg ctcaacaagc ctgattccta cattctcgcc attgtgaaat tcaaggccga
     2581 tggaagccac acgctccact atattaccca gccttttgca aaagagcccg acattggcaa
     2641 gcgcacgcgt aactacgata tccagatgat gctggaaaag gggacaaggc aatagtgata
     2701 cacctgctag aagctcaata ctttagagca tttgacgaat aacaaggcca ccgctaagta
     2761 catatagaga aatatattat tgatttattt caaaatagtg tcgatagata gcacttattg
     2821 tgataaaata gcgatgcaca atagcactga attctacact tgttgggaaa ttttaaaact
     2881 atcaaaatct tcattgacag tacaaaggaa atagactata ttagtcatag caaacctgcc
     2941 aggcctctgc gaaagcatgc caaaattggc gggtttttgt ttatgtagcc aaaatggaat
     3001 ataaaaagcc accgctgaac tatgaagctc aagcaaaact tctccttaag cgcggtcttg
     3061 ttgcagatga agccattcta ataaaacatc taaaatctgt aaattattac cgactctctg
     3121 gctatctcta cacattccgt ataatcgatg ataatggcaa ccatcttgat tcctattatc
     3181 cagatacatg ctttgatacc gtatggcagc gatatacctt cgacaggcaa ctgcgcctca
     3241 taattttaga tggaattgaa cgcattgaga ttgcgttgaa gacaaagctc acgcaggttt
     3301 tcagtatgaa gtatggtgca tttggatatc tcgatattaa gaatttgcca aggttttcac
     3361 aagatagaca ctcgatcttt attactaagc tccgtgaaga aactcaaaga tcaaacgaag
     3421 atttcgttca gcatttcttc aaaaaatatg gagactatca taatgatctt cccttatgga
     3481 tggcaacaga aataatgagc tttggatcat tgttgacgct attgaatggt atggcagaac
     3541 aagatttgaa agtcattgcc cgagaattca atctgaatgt gcctattctc gattcttgga
     3601 ttttaacatt gagttcgatt agaaatatct gtgctcatca tggtcgcttg tggaaccgcg
     3661 agttaggtat taaacccaaa ataccttctg gttctaaata tgcagaatgg cattctccta
     3721 ttgaaacagg caataagaga atttttggtg tactcaccat aataagatat atgttgcgct
     3781 atattgctcc tcaaagtgca tggccgcaaa gattcatgaa tcttttgtca caatacccag
     3841 aaattccgct taactttatg ggtttcccaa agaattggga aacccacgcc atttggaaat
     3901 atcacgagta atgctaatgg ttttgtttcc ttatatcggc actataatga gccttagaat
     3961 ttagaccatt gtcgatgaaa aataaggtgg acaggagcct tcttgccaag aatgagaaag
     4021 gaggcataga agttcactat gtgcacacac tacgacaagg aattcaaggc gaaagtcgcc
     4081 cttgaggcgg tcaagggaga atcaagggag agaagatgat tcaggagctc gcaacactct
     4141 atagggtgca tccgaatctt gtagccttat ggaaaaagca gcttcaagag aatgcaccgg
     4201 agctgtttga gagaacccag aaggataaaa agaaggaagt agcacagcac aaagaagagg
     4261 agctatataa gtagattgag caacttccgg tagagaatga gtttttaaaa aaaattacaa
     4321 acagttgtac gggaccgaac cgcaatggta gagccgaagc atcctgagct ttcgattcgt
     4381 cggcaatgtg tgctgtttgg tatctccaaa gggagttact actataaaac gaaagcaaga
     4441 agaacaaagg aggagaatga ggtttgtatg cttgggcata tccttgaggt actaaagtag
     4501 acaccgttct atg
//
LOCUS       DAOOWY010000134         4467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_19550_length_4467_cov_12.394832, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000134 DAOOWY010000000
VERSION     DAOOWY010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19550_length_4467_cov_12.394832"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PK105_08100"
     CDS             <1..760
                     /locus_tag="PK105_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU61716.1"
                     /translation="AHIGLIYDDPIEQLLVCQKAKLAGVSRIVSICNSLVDFKQVYEN
                     LKPAEYVYHAVGVSPSEVNNPGKDWQRFIEEASKLSRVIAIGEIGLDYYHKFGDRRSQ
                     IELFIEQLEIAARLGLPVIIHNREAGKDVLDILKDRLPPAGAVLHCYSENAAYAADIL
                     DLELDIYFSFAGNLTYRNARNLHETVAFLPLDRILLESEAPFMVPAEYRNKRNMPEYL
                     PSTAQFLADFLNIDVEEVAETTYQNACQFFKLAP"
     gene            798..1847
                     /gene="dusB"
                     /locus_tag="PK105_08105"
     CDS             798..1847
                     /gene="dusB"
                     /locus_tag="PK105_08105"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOU61717.1"
                     /translation="MKKAIPFSMNFDSRNVPFFLAPVAGYSDVAFRAICFEMGAALCY
                     TEMISAEALVRGHPKTKLLLARDSSERYFAIQLFGSNPTTLAKAARIAADYKPVVIDL
                     NCGCPVPKIIKAGAGAALLRTPERIGEIVRAMRDQTSVPISIKMRTGWDQDSINYLEV
                     AAIAVQAGVCAITLHGRTRAQGYSGKADWEAIKVLSEQTSVPVFGSGDVLNAGAAISM
                     IRETRCDGVMVARGAIGNPFIFSDLNELDNPKEAEAYSYKPPSLFIENSSCPERTLLG
                     LRNPGIIAKTALHHLEMSARYLGERTACIEFRKHFCAYTRGLPGGSELRAKAVRCSTI
                     DEYKPLIEIIAKAPT"
     gene            complement(1871..3211)
                     /locus_tag="PK105_08110"
     CDS             complement(1871..3211)
                     /locus_tag="PK105_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61718.1"
                     /translation="MRNEKLYRRCAFRIGIGIFALLIFFLCTACANLAPRALVWTDMP
                     ELIIAAQLFNRENDRYIIDVEYKAFPASEIIKTTKPPSLIIARYMLSNLVMKKFASLD
                     ELFSEYYLDPNDMYPALLNAGKLGSKHVLLPVSFDCELIIENKSETARAGTAMLDPET
                     IRQTSQAFTRIENGKISSMGFSPRWDLGFAENWLLAANVGFTPNEKWKPTSVPAISDD
                     ISSWPVLWNKQNLEKCIEGLLSFNSDIGVSQQDDFAFTYFNMPGYQLVLEGKVLYWPM
                     KASEYFKLPHSIREQLRYRFPAVDHKIILTENSRFIGIPRGSKNRKAALEFARWLFVA
                     TNQEKIWKEMESQQLLPDYVGAFDGFSSIMQVNETIFLRYFPEYGQNPLLPAAIPEPA
                     RIPDYWEDFSRDFLHHWLEAVMSNPESRVAIDQGFRSAFEQYMGIMPDWLISNH"
     gene            3282..4370
                     /locus_tag="PK105_08115"
     CDS             3282..4370
                     /locus_tag="PK105_08115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU61719.1"
                     /translation="MQKSFDSIERARINWHSQCLRVRYGEKVWRLGIDAGFTCPHRSV
                     DRLHGGCRFCASDGNVSAYQKSQTFKSKTSVPNTINANVNASNHIDDGSTIEQQIRRA
                     LAFTQRRYKAKAFFLYFQAYTCTNAPVPMLARIYDNAIATFERLYYDAQKQAGMDLQL
                     SNLSKTPLKGIIVSTRPDCFDIAKAELLASYRKQGFEVWVEFGLQSMHEETLALINRG
                     HGIDEFLNAMDIAKKAQLSRTVHLILGLPGESHGSMIETAKFVAQIKPEGLKYHDLHL
                     VKGSAFARSFCSGEIALMHPSRLPALLADCLELMPASTEIMRLSADFRHDETINLHPQ
                     VDKHKLARAVEEELAVRDSFQGQRFEFD"
BASE COUNT         1300 a          994 c          946 g         1227 t
ORIGIN      
        1 cgcccacatc gggcttattt atgatgaccc gattgaacag cttcttgttt gccaaaaagc
       61 taaacttgcc ggggtcagtc gcatagtcag catctgcaac agcctcgtgg acttcaaaca
      121 ggtatatgag aaccttaaac ctgctgaata tgtctaccat gctgtcggtg tatcaccatc
      181 agaagtaaac aatcctggta aggattggca gcgctttatc gaagaagctt caaagctttc
      241 aagagtaatc gcaataggag aaatcggact cgattattat cacaaatttg gtgatagacg
      301 cagtcagata gagctcttta tcgagcagct agaaattgcc gcaaggctag gtctccctgt
      361 cattatccac aatcgagaag ctggtaaaga tgtgctggat atcctaaagg atcgactgcc
      421 acctgcagga gcagttctgc actgctattc ggaaaatgca gcttatgcgg cagacatcct
      481 cgatctcgaa ctggatatct atttttcatt tgcaggtaat ctcacttata ggaatgcgag
      541 aaatctgcac gaaacagttg cattccttcc tctagatcga atattactag aaagtgaagc
      601 gccattcatg gtacctgcag agtatagaaa taagcgcaat atgcctgaat accttccttc
      661 cacagcgcaa ttcctggctg attttttaaa catcgatgtg gaagaagtgg ctgaaacaac
      721 ataccaaaac gcttgccagt tctttaagct tgcgccttag aaattatatt gcttggataa
      781 agagatttga aaagcaattg aagaaagcga tccccttttc gatgaatttc gactcacgga
      841 atgtgccatt ttttcttgcg ccggtggccg gttattctga tgtggctttc agagccatct
      901 gttttgagat gggagctgcg ctctgctata cagaaatgat tagcgcggaa gcactggtcc
      961 gaggtcatcc taaaacaaaa ctccttctgg cccgcgattc ttcggagcga tattttgcta
     1021 tccagctttt cggttccaac cccacaacat tagcaaaggc tgcccgaatc gcagctgatt
     1081 ataaaccagt tgtaatagat ctgaattgtg gctgtcctgt tcccaaaatc atcaaagcag
     1141 gagcgggagc agcattgctc agaactcccg aacgcatagg agaaatcgta cgtgcgatgc
     1201 gcgatcaaac ctccgtcccc atttccataa agatgcgtac tggctgggat caagactcga
     1261 tcaattatct cgaagtcgca gccattgccg tacaagcagg agtatgcgct ataacactac
     1321 atggccgaac acgcgcacag gggtattcag gcaaagcgga ctgggaagca attaaggttc
     1381 tctctgagca aacctctgtc cctgtatttg gttctggcga tgtcctaaat gcaggcgcag
     1441 caatatccat gatccgcgag acacgctgcg atggtgtcat ggtagcaagg ggagctatag
     1501 gtaatccttt cattttctcg gatctgaatg agttggataa tcccaaagaa gccgaggcat
     1561 attcttacaa acctccgtcc ctgttcattg agaattcttc ttgcccagag agaaccttat
     1621 tgggacttag aaacccaggt attatagcta aaactgctct tcaccatctc gaaatgtctg
     1681 ctagatatct cggagagagg acagcatgca tcgagtttcg aaagcacttt tgcgcttaca
     1741 caagaggtct tcctggagga agtgaattac gtgccaaagc ggttcgctgc tctactattg
     1801 acgagtacaa gccccttatt gagataattg ccaaagcgcc tacctaaatt gatcttttca
     1861 aaagataata tcaatgatta gaaatgagcc aatcgggcat tatacccata tattgctcaa
     1921 aagcacttcg aaaaccctga tctattgcta cccttgattc aggattagac atcactgctt
     1981 cgagccaatg atgcaaaaaa tctctcgaga agtcttccca ataatctgga attcgggcag
     2041 gctccggtat ggcagctggc aataatgggt tttgtccata ttcaggaaag tatcgtagga
     2101 atatagtctc atttacttgc attatactgc tgaaaccatc aaaggcgcct acataatcgg
     2161 gcaaaagctg ctgtgattcc atttctttcc atattttctc ctggtttgtt gcgacaaaaa
     2221 gccatctagc gaactctaac gctgctttcc gattttttga gcccctagga attccaataa
     2281 atctcgaatt ttcggttaat atgatcttat ggtctaccgc tggaaagcga tagcgaagct
     2341 gttccctgat tgagtgagga agcttgaagt attcgcttgc cttcataggc caataaagca
     2401 cttttccttc aagaacaagc tgatatccag gcatgttgaa atatgtaaat gcaaagtcat
     2461 cttgttgaga tactcctata tcagaattaa aagataacaa gccttcaata catttttcaa
     2521 gattttgctt gttccataaa accggccatg agctaatatc atctgatatg gcggggacag
     2581 aggtcggctt ccatttttca tttggagtaa aaccgacatt tgctgcaaga agccaattct
     2641 cagcaaaacc cagatcccac cttggagaaa atcccatgct gctaatcttg ccattttcta
     2701 tccgagtaaa agcttgagat gtttggcgaa tcgtctcggg atcaagcatg gctgtacctg
     2761 ctcttgcagt ttcggatttg ttctcaataa tcaattcgca atcaaaagaa acaggtaata
     2821 aaacatgctt tgaacccaat ttacctgcat tgagcaaggc tggatacata tcgtttggat
     2881 caagataata ctctgaaaaa agttcatcca atgaagcgaa ttttttcatc acaagattcg
     2941 aaagcatgta tcttgcaatt atcagggacg gaggtttggt cgtcttaatt atctcggaag
     3001 cagggaacgc tttatattca acatcgatga tataccgatc gttctctcga ttaaaaagtt
     3061 gagccgcgat tatcagttcg ggcatgtctg tccaaacaag agcccttggg gctaaatttg
     3121 cacatgcagt gcatagaaag aatatcaata gagcaaatat gccaatacca atgcgaaatg
     3181 cgcatcgtct atacaatttc tcgttcctca cataatcttc atggatagtg ctatcatagt
     3241 aaagaatcaa ttgcatgcaa agaagaaggt caagctagat tatgcaaaaa tccttcgact
     3301 cgatagaaag agcaagaatc aattggcact ctcaatgcct aagagttcgt tatggtgaaa
     3361 aagtatggag attaggcatc gatgcaggtt tcacttgtcc acaccgcagt gtagatcggt
     3421 tacacggagg gtgccgtttc tgtgcttcag atggaaatgt ctcagcttac cagaaatcgc
     3481 aaaccttcaa gtcgaaaacc tctgtcccca ataccattaa tgccaacgtc aatgcaagca
     3541 atcatatcga tgatggatcc actatcgaac aacagatccg ccgtgcgctt gcctttactc
     3601 aacgacgtta taaggcaaaa gccttctttc tctactttca ggcatacacc tgtactaacg
     3661 cgccagtccc tatgctcgcc agaatatacg acaacgctat agcgacattt gagcgccttt
     3721 actatgatgc tcaaaaacaa gctggcatgg atcttcagtt atctaatttg tctaagacac
     3781 ctttaaaagg aattattgta tcaacaagac cagattgttt tgatatagcg aaggcagaac
     3841 tgctagcatc ctatagaaaa cagggttttg aagtatgggt cgaatttggc cttcaatcaa
     3901 tgcacgagga aacactcgct cttattaatc gaggccatgg tatcgatgaa tttctcaatg
     3961 ccatggatat tgccaaaaaa gctcagctta gtagaaccgt acatttaatt ttaggattac
     4021 cgggagaatc tcacgggtct atgattgaga ctgctaaatt cgttgcccaa ataaaaccag
     4081 agggattgaa atatcatgat ttacatctag taaaaggatc agcattcgcg cgaagtttct
     4141 gctctggcga gattgcattg atgcaccctt ctcgccttcc tgctcttcta gctgactgtc
     4201 ttgagctaat gcctgcatcg actgagatta tgcgtctgtc agctgatttt cgacatgatg
     4261 agacaatcaa tcttcatcca caagtcgaca aacataaact ggctcgagca gttgaggaag
     4321 aacttgcagt tcgtgattca tttcagggac agaggtttga atttgactga aagaagagcc
     4381 atcgatgata gtcatttcag cccttgacaa ttgccatgct ttctcgtatt gtcgattctc
     4441 gccgactggt ctgctgacat tggagcg
//
LOCUS       DAOOWY010000135         4341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_20276_length_4341_cov_20.536164, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000135 DAOOWY010000000
VERSION     DAOOWY010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20276_length_4341_cov_20.536164"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PK105_08120"
     CDS             complement(<1..721)
                     /locus_tag="PK105_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61720.1"
                     /translation="MNQKYEKLKGLLQELFQLDQPDLDFGIYRIMHAKSAEVTKFLDE
                     DLLPQVEAAFAQYKTADKSELEAQYRRAIEQAKELGFSNPEEAPKVKELKEKLDTVAV
                     DTGALESEVYDHLYSFFRRYYSEGDFLSKRVYKPGVYAIPYEGEEVKLYWANYDQYYI
                     KTSEYLRDYAFRLRPENTANPMRVHFKLVDAAEGEHDNVKEAQGKERKFKLAIGNPLS
                     LESGELVIHFTYEPAEEKQKDL"
     gene            complement(746..3811)
                     /locus_tag="PK105_08125"
     CDS             complement(746..3811)
                     /locus_tag="PK105_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOU61721.1"
                     /translation="MFAITPYHAKYFAWDLTRHAPEGLDRLSMSLFDAKVDLNPHQIE
                     AALFALRSPLSKGVILADEVGLGKTIEAGLVICQYWAERKRKILVVCPASIRKQWAME
                     LEEKFNLPAVVLDAKSLRQAQRALKYSKEEEIAGANLASNPNTSTPLFDRPAISIVSF
                     NFAYAMRSEIRAQNWDIVVIDEAHKLRNAYRTQNKMGQAIRWALEGHKKILLTATPLQ
                     NSLMELYGLSILIDDQIFGSSEAFRARYANGSSDLTELRRRLSAFCTRTLRKQVSEYI
                     RYTERHALTQPFRPNDEEQDFYNAVSDFLRRKDSYSLPKAQRHLTSLILRKLLASSTK
                     AIEATLGMLLARLERLRVFVEEEKETTAREMARAQASAKVLSTLIDDPEFADRLLDGE
                     DMESELADSLPETDAASDIDASQALNSAKPAASTALDIRNSIDIAQLDDEIRVLHGLI
                     ESAKQIREDTKSKALLTALELGFSQMKTLGAARKAVIFTESRKTQAYLKSYLDAHGYA
                     GQIVTFNGSNNDPESIAIYTQWLEENRDSGRTSGSRDIDMRTALIEHFRDHATLMVAT
                     EAAAEGINLQFCSLVVNYDLPWNPQRVEQRIGRCHRYGQKYDVVVINFLNERNQADRR
                     VLELLSEKFKLFDGVFGASDEVLGSIESGIDFETRILEIYQECRTTDEIDAAFRALQE
                     ELDAQIQSRLEDARHKLLEYFDEDVHDRLRLRLADAQMQLDRVGRQFWQLTQWALVDG
                     ARFYNEELAFDLEKSPSPQIARGRYHLVRKPSDTKQPEWAYETDRFLYRISHPLGEYV
                     IEKAKNADCPPAEIIFDVTHHPTKLSVVEALKGEAGYLVLQRLVVESYEQEEYLLFSG
                     FTDDGKPLDQETMERLFLCEGKVHGPIIISPHDKDHLALEAERCATATISRSLERNNQ
                     HFLEECDRLDRWADDMVNAAEEALSDTKEQLKALRRQSRQAATLQEQHEIQQKIAKLE
                     RQRHRQQQEIFKVEDEIMTKRDALIDDLEKRLAQKTAAEQLFTIRWVVK"
     gene            3921..4316
                     /locus_tag="PK105_08130"
     CDS             3921..4316
                     /locus_tag="PK105_08130"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOU61722.1"
                     /translation="MERARTAREGVEILTKLTEKYGQAWNSIMFEIGDPQELWIVEIA
                     NKQWVARRYTNCYTVRTNQFQITDDYEICSDDLITYAQAQGWLSRNVPNTQKINFRAV
                     YGTIELYPSDNENFASRPAVETLYNTQVR"
BASE COUNT         1049 a         1076 c         1078 g         1138 t
ORIGIN      
        1 tcaagtcctt ttgtttttct tccgctggtt cataggtaaa atggatgaca agctcgcctg
       61 actcgagcga tagaggattg cctatagcca gcttgaactt tcgctctttg ccctgcgcct
      121 ccttcacatt atcgtgctcg ccctccgcgg catcaacaag cttaaaatgc acgcgcatcg
      181 gattggccgt attctctggg cgcaatctga acgcatagtc ccgcaaatac tcacttgttt
      241 tgatgtaata ttgatcgtaa ttggcccagt agagcttaac ctcttcgcct tcgtacggga
      301 tggcatacac gccaggtttg tagacccgct tggaaagaaa atcaccttcc gaatagtagc
      361 gcctgaaaaa gctatacaga tggtcgtaca cctcgctctc gagcgcaccc gtgtctaccg
      421 caacagtgtc gagcttctct ttgagttctt tgaccttggg cgcttcctcc ggattggaaa
      481 aaccgagttc ttttgcctgc tcgatggcgc gcctatactg agcctcaagc tcgcttttat
      541 cagcagtctt atactgcgca aaggctgcct caacctgcgg caaaaggtcc tcgtccagga
      601 acttcgtcac ttctgcgctc ttggcgtgca tgattcggta gatgccaaag tcgaggtcag
      661 gctgatcgag ctggaaaagt tcctgcagaa gacctttcag tttttcatat ttctgattca
      721 tgttcatatc ctctcatgaa atgggtcatt tcacaaccca acggatggta aaaagctgtt
      781 cagccgcggt tttttgagca aggcgctttt caaggtcatc aataagggcg tcgcgcttag
      841 tcataatctc gtcctccacc ttaaagatct cttgctgctg acggtgccgc tgacgttcaa
      901 gcttggctat cttctgttga atttcgtgct gttcttgcag tgttgcagct tgtcgagact
      961 gtcgcctgag ggctttgagc tgttccttag tgtcggaaag cgcctcctct gcggcattca
     1021 ccatatcgtc cgcccatcgg tcgagtcggt cgcattcttc aagaaaatgt tggttgttcc
     1081 tttccaatga tcgactaatc gtagctgtgg cgcagcgctc tgcttctaga gcaaggtgat
     1141 ccttatcatg tggagaaatg atgattggcc catgtacctt tccttcgcag agaaagagtc
     1201 tttccatggt ctcctgatca agtggcttgc catcgtcggt aaagccagag aaaagcaaat
     1261 attcctcctg ctcatacgat tcgacgacaa gcctctgaag cacgagatag cctgcctcac
     1321 ctttgagcgc ctctaccacg ctcagtttgg tagggtgatg ggtcacatcg aaaatgattt
     1381 cggctggcgg gcaatctgcg ttctttgcct tctcgatgac atactcacca agtggatgag
     1441 aaattctata gagaaaacga tcggtctcat aagcccattc tggctgcttt gtatcgctcg
     1501 gttttcttac gagatggtaa cgacctcggg caatttgcgg agatggcgat ttttcaagat
     1561 caaaagcaag ctcctcattg tagaatcgag cgccatccac gagtgcccac tgtgtcagct
     1621 gccaaaactg cctacctacc ctgtcaagct gcatctgagc atccgccagc cgaagccgaa
     1681 gtctatcgtg gacatcttcg tcaaaatact cgagtaactt gtggcgggca tcctcaaggc
     1741 gcgattgaat ctgtgcatcc aactcttcct gcaaagctcg aaatgctgca tcaatttcat
     1801 cggtggtgcg gcattcctgg tagatttcga ggatgcgcgt ttcaaaatcg atgccagact
     1861 caatactgcc taacacctcg tccgaagcgc caaacacgcc atcgaacagc ttgaatttct
     1921 cagagagcag ctcgagaaca cgcctgtcag cctggttgcg ctcatttaag aaattgataa
     1981 caaccacgtc gtacttttgg ccataacggt gacatcgtcc aatacgttgc tcgacgcgtt
     2041 gaggattcca tggtaaatcg taattgacga caagcgagca gaattgcaag ttgatacctt
     2101 cggccgccgc ttcggtggcc accatcagcg ttgcatgatc gcggaaatgt tcgataagcg
     2161 cggtgcgcat gtcgatgtct cgcgagccac ttgtgcggcc agaatctcga ttctcctcta
     2221 gccattgggt atagatggca atggattcag ggtcgttatt cgagccatta aaggtgacaa
     2281 tctggccggc atagccatgc gcgtccaaat aggatttaag ataagcttgg gtttttctcg
     2341 actcggtgaa gatgacggct ttgcgtgcag ctccgagggt tttcatctgc gaaaaaccca
     2401 attcgagcgc agtaagaagc gctttagact ttgtgtcttc gcgtatctgt ttcgcacttt
     2461 ctatgaggcc atgcagcacc ctgatttcgt catcaagttg cgctatgtcg atactatttc
     2521 tgatatcgag tgcagtgctc gcagcaggtt tagcagaatt gagcgcctgc gatgcgtcga
     2581 tatctgaagc ggcatcagtc tcaggaaggc tgtctgcaag ctcgctttcc atgtcttcac
     2641 catcgagcaa tcgatccgca aactcaggat cgtcgataag cgtgctgaga acctttgcgc
     2701 tggcttgcgc cctagccatt tctctagctg tagtttcctt ctcctcctcg acaaatacgc
     2761 gcaggcgctc tagtcgggca aggagcatgc cgagtgtcgc ttcaatagcc ttggtcgaag
     2821 aagcaagaag tttgcgaagt atcaaggaag taaggtgtcg ctgcgctttt ggcagggaat
     2881 agctgtcctt tcgccgcaaa aagtccgaaa ctgcattata gaaatcttgt tcttcatcgt
     2941 tcggtctaaa aggctgagta agtgcatggc gttcggtata acggatatat tcgctcactt
     3001 gtttacgcaa ggtgcgcgta caaaatgcgg aaagacgacg ccgtagctcg gtgaggtccg
     3061 agcttccgtt ggcatagcga gcgcgaaaag cctcagagct tccgaaaatc tgatcatcga
     3121 taagtataga aagaccatag agttccataa gggagttctg gagcggtgtt gccgtaagaa
     3181 gaatcttctt gtggccttcc agcgcccagc ggatagcctg tcccatttta ttttgcgttc
     3241 gataggcgtt gcgcaattta tgcgcttcgt caatgacgac gatatcccag ttttgagccc
     3301 gtatttcgct gcgcattgca tacgcaaaat tgaacgacac gatactaata gcgggccgat
     3361 cgaatagggg agtagaagta ttgggattag aagctaaatt ggcccccgct atctcttctt
     3421 cctttgagta tttgagcgcg cgctgtgcct gcctcaaaga tttcgcatcg agaaccaccg
     3481 cgggcaggtt gaatttttcc tcgagctcca tagcccactg cttgcggatc gatgctggac
     3541 acaccacaag gattttgcgc ttgcgctcag cccagtattg acagatgaca agcccagcct
     3601 cgatggtctt gccaagccct acttcgtcgg cgagtatcac gcccttggag agcggcgagc
     3661 gcagtgcaaa gagtgctgcc tcgatttggt gagggttgag gtcgaccttg gcatcgaaaa
     3721 gagacataga caggcggtcg agcccttcgg gcgcatggcg ggtcaaatcc caggcaaagt
     3781 atttggcatg ataaggtgtg atggcgaaca tcaggaggaa tcctggattt aattatagac
     3841 tgagaaattt gtaaagcaaa tggctttaat gaagcaatcc aaaggcacta tgaagcattt
     3901 tgcccataga ggctaatatt ttggaaagag cgaggacagc acgtgaagga gttgaaatac
     3961 tcacaaagct cactgaaaaa tatggtcaag cctggaattc catcatgttt gaaattgggg
     4021 atccccaaga gctttggatc gtcgagatcg caaacaaaca atgggtcgcg cggcggtata
     4081 caaactgcta cacggtaagg acaaatcaat tccagatcac cgatgattat gaaatttgct
     4141 ccgatgacct tatcacctat gcacaggcac aagggtggtt gagtaggaat gtcccaaaca
     4201 cccaaaagat aaattttaga gcagtctatg gcacaatcga gctttatcct tcggataatg
     4261 aaaattttgc ttcacggccc gctgtggaga ctctctataa tacgcaagta cggtagatac
     4321 tgtagaggta tcgatattcc c
//
LOCUS       DAOOWY010000136         4316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_20436_length_4316_cov_10.442854, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000136 DAOOWY010000000
VERSION     DAOOWY010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20436_length_4316_cov_10.442854"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PK105_08135"
     CDS             <1..704
                     /locus_tag="PK105_08135"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456497.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOU61723.1"
                     /translation="MVDMAHIAGIVAAGRHPSPIANAHFITTTTHKTLRGPRGGMILV
                     GRDTENDRGIMNAKGDALRRWSELIDSMVMPGIQGGPLMHVIAGKAVAFKEAQRPEFV
                     EYIDHVLSNAKVMAQALTLGGARIVSGGTDNHLMLVDLTPLGINGRDAERWLDEASIT
                     VNKNSIPFDKKSAFVTSGIRIGTPAITTRGMGPSEMEQIASFVMEVLRSKGDPQVIAR
                     IKQEVIGLTSRFPLP"
     gene            712..1950
                     /locus_tag="PK105_08140"
     CDS             712..1950
                     /locus_tag="PK105_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOU61724.1"
                     /translation="MQKIEHSELYEQAGRQAAYRLLSNAEEQGFFSYGILTRQAWEQI
                     VEEQGTVQADCREGKPLSLNSLRTSSPWTEQLKAESSPVMALKERYHLSDVGAMLVVA
                     LRYAPDVEAEEAAARALRGLPPLPLARDGRPAARVARFARGNWYREILDRLRNCATAT
                     FGAIIKEGSAERLGLEAKPPQRWPRFVNSIFPEKALALAAGLGTIGRNGLLVAKKTNN
                     SDIFDALDFQEHLIASDSSKPTTNPPTWSSAVLIGLMLLPFDFDMLKLEKKDIFGGVA
                     PSFFAVCGNCRLCIESCPSRALSFSSLPGFARHLCIQNYSSIDGNLPDFIEKSWADQL
                     YGCDLCLEACTYFEPDQKAHTNRGRISGFFDAESLADLSEEQIQALFKASALDQKWIA
                     PGALKRNAAIVIQKFHKLRN"
     gene            2129..2500
                     /locus_tag="PK105_08145"
     CDS             2129..2500
                     /locus_tag="PK105_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61725.1"
                     /translation="MKKIIAVFAIISIAFFACAPKKTNIDNQDSKSVSNMAPEEIKDV
                     ATWKAAYDDLISAYSSAVAQLNGGNLGAGAKIEELGKRAEALNSVAETIRSGLSGQEQ
                     ADFAQVIEAYREKFSQAVNSN"
     gene            2636..3598
                     /locus_tag="PK105_08150"
     CDS             2636..3598
                     /locus_tag="PK105_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61726.1"
                     /translation="MNIDLINLRSRMISIVREFFISKDYLELDTPALAPYLIPESCLE
                     VFATEYIHPIYGHRPLYLIPSPEIWMKKVIANTNRSVFQICKAWRNAESVSPIHNPEF
                     TMLEYYGVGLSSKDNIEITEELFEALAGTETPSRVRPPFRVMSMKEAWAEFAHADLDA
                     LGDDEAMREACRRRGLLVPQTAAWEDAFHILFLSIVEPSLPKDKPLILDLYPSRIECL
                     AADIPGTRYKERWELYVEGIEIANCFTEMADPEAVDRYMQSQSKKKRESLVPHIIDEA
                     YSSIFKDFPPCSGVALGFERLMMVLCGKKSIEDVMIFPFSSFFG"
     gene            3759..>4316
                     /gene="efp"
                     /locus_tag="PK105_08155"
     CDS             3759..>4316
                     /gene="efp"
                     /locus_tag="PK105_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOU61727.1"
                     /translation="MIRGGDIGIGTCLIINGVPHIVVEREFVNPGKGAAFARVRAKNL
                     KNGYVVTQTIKTADSVEDAQVDLVDCQYQYSDDESFHFMNNETFEQFEVPITAHEDRA
                     PYLKEGETYTIVMWEGEPIDIKIPYKMVFTVAESENYVRGDTVSGATKPVRTETGLVV
                     RVPLFIKQGEKILINTETNEYVERVN"
BASE COUNT         1171 a          929 c         1099 g         1117 t
ORIGIN      
        1 ttatggtcga tatggctcat attgccggca tagtggccgc tggcagacac cccagcccca
       61 ttgcgaatgc tcattttatt acgactacga ctcataagac cttgcgaggt ccgcgaggcg
      121 gtatgatcct ggtgggcaga gatacggaaa atgatcgtgg cattatgaat gctaaaggag
      181 atgcactgcg cagatggtcg gagcttatcg acagcatggt catgccagga attcaaggcg
      241 ggccattgat gcatgtcatt gctggtaaag cggttgcatt taaggaagct cagaggcctg
      301 agtttgtaga gtatatcgat catgtgcttt ccaacgcaaa ggtgatggca caggctctaa
      361 ccttgggagg agctagaatt gtatcgggag gaaccgataa tcacctcatg cttgtcgatc
      421 tgacacctct tggaataaat ggtcgagatg cggagagatg gctcgatgaa gcgagcataa
      481 ctgtgaataa gaattcaatt ccttttgata agaaaagcgc gtttgtgacg agcggaatac
      541 gtattggtac gccagctatt acaactcggg gcatgggacc atcagagatg gagcagatcg
      601 cttcatttgt tatggaagtg cttagatcca aaggcgatcc tcaggttatt gcgcgaatca
      661 agcaggaggt cattggtctc acctcacgct tccctctgcc ataaagctgc aatgcagaaa
      721 atagagcact cagaactcta tgagcaagct ggtaggcaag cagcgtatcg gcttctttcg
      781 aatgcagagg aacaaggttt tttttcttat gggatactta cacgacaggc atgggagcag
      841 attgtagaag agcaaggtac ggttcaagca gactgccgcg aagggaagcc tctaagtcta
      901 aattccttaa ggacttcgtc gccatggacc gagcagttaa aggcagagag cagtccagtc
      961 atggcattaa aggagcgcta tcatctttcg gatgtgggag cgatgttggt ggtagcgctt
     1021 cgttatgcac cggatgtcga ggccgaggag gcggcagccc gcgcgctgcg cgggctgccg
     1081 cctctgccac ttgctcgcga cggccggcca gctgcgaggg tggcaaggtt tgcgcggggc
     1141 aactggtata gagagatctt ggatcggctc cgcaattgtg ctaccgcgac ctttggcgca
     1201 attataaaag agggctctgc ggaaaggctt ggtctcgaag caaagccacc tcagcgatgg
     1261 ccgagatttg tgaattcgat atttccggaa aaggccttag ccctcgcagc tggccttggg
     1321 acaattggac gaaacggcct tcttgtcgct aagaagacaa acaactcgga tatctttgat
     1381 gctcttgatt ttcaagagca cttgatagca agcgactcca gtaagccaac aacgaatccg
     1441 ccaacttggt cttccgctgt tctgatcggt ttgatgcttc ttccattcga ctttgacatg
     1501 ttgaaactcg aaaaaaaaga tatttttgga ggtgtggctc catcattttt tgccgtctgt
     1561 ggcaattgca ggctttgtat cgaatcctgc ccaagcagag ccttaagctt ttcatccctt
     1621 cctggctttg cgcgacatct ctgtatccag aactattcat cgatcgatgg gaatctgcct
     1681 gattttatag aaaagtcctg ggcggatcag ctttatggat gcgatctttg ccttgaagcc
     1741 tgtacatact ttgagccaga tcaaaaggca catacaaatc gtggtcgaat aagcggtttt
     1801 tttgatgctg agtcgttggc agatctcagc gaggagcaaa tacaagcttt gttcaaggct
     1861 tcagctctag atcaaaaatg gattgcacct ggagcgctca agagaaatgc ggcgatagta
     1921 atccagaagt tccataaact taggaattga agcgagctgc atgacaattt tatgcaaaga
     1981 tagccggcag catatcttcg agcccaataa ttcttatcgc ggtgactgag tctattgcag
     2041 cgcagcgcaa taatggcatt ggcgtctttt attgacatat tatgttaaat aatatataat
     2101 gccgctgttt tgtgcaggag tatgtaaaat gaagaaaatc attgcagtgt tcgcaatcat
     2161 atctattgca ttctttgcat gtgctcccaa aaagaccaac attgacaatc aggatagcaa
     2221 aagtgtctcc aatatggctc ctgaggagat caaggatgtg gccacatgga aggctgccta
     2281 cgacgatctt atcagtgcat attcaagcgc ggtcgctcaa ctcaacggcg gcaatctggg
     2341 ggcaggcgca aagatcgaag agctagggaa gcgagctgag gcacttaact cagtagcaga
     2401 gaccattaga tctggcctct ccggtcagga acaagccgat tttgctcagg tcatcgaagc
     2461 ttatagagaa aaattcagcc aagcagtaaa ttctaattga aatggcccat aagatgaaag
     2521 gctgagaaat agtctgccat ggtagtccat ggtagtagca agaggtcgaa tcctaagcct
     2581 gggtctgccc tatgctcatg tctcattgtg ataagggcgg gatagagtag ctaatatgaa
     2641 tatagatctg ataaacctgc gctctcgtat gataagcatt gtccgcgagt ttttcatttc
     2701 gaaggattat ctcgagctag acacgccagc gcttgcgcct tatctgatac ccgaatcttg
     2761 cctggaagtt tttgctacag agtacattca tcccatttat ggccataggc ctctatatct
     2821 cattccttcg ccggaaatat ggatgaagaa agtcatcgct aatacaaata ggagtgtctt
     2881 tcaaatctgt aaggcctggc gcaatgcgga atcggtttcc cctattcata atcctgagtt
     2941 tactatgctt gaatactacg gcgtgggcct ttccagcaaa gacaatatcg aaattacgga
     3001 ggagcttttt gaagcccttg ctggcacaga aactccttct cgagttcgtc cgccctttcg
     3061 agtgatgagc atgaaagaag catgggctga gtttgctcat gcggatctgg atgcgcttgg
     3121 cgatgatgag gccatgcgcg aagcttgtcg acgccgtgga ttattggtac ctcaaactgc
     3181 cgcctgggaa gacgccttcc atatcctttt tctcagcata gtcgaacctt ctcttcctaa
     3241 agataagcca ctcattctag acctttatcc ttcgcgtatc gaatgtcttg cagcggatat
     3301 tcctggcacc agatacaaag agcgatggga gctatatgta gaaggcatcg aaatcgcgaa
     3361 ttgctttacc gaaatggctg atcctgaggc tgtcgatcga tatatgcagt cgcagagcaa
     3421 aaagaagaga gaatcgctag tgcctcatat tatcgatgag gcatatagct cgatttttaa
     3481 agactttcca ccatgttccg gagttgctct tggctttgag cgcttgatga tggttttatg
     3541 tggcaagaaa tcgatcgaag acgtgatgat ttttcctttc tcgtcctttt ttggctgaga
     3601 caaatagaaa aagttatcca tgaaatgatg tagataaaga tataagctat aataaagtcc
     3661 tatgatgaaa acagtcgcag gaggctttag tctttacata gagcaagatt ccaagctagt
     3721 atagtttgat ttaatgcttt tgtatcgagg aaaacattat gattcgtggt ggtgatatcg
     3781 gaatcggaac ttgtctcatt atcaatggcg tgccacatat tgtcgtagaa cgagagtttg
     3841 tcaatccggg gaagggagcc gcttttgctc gcgtgcgcgc gaagaatctc aaaaatggat
     3901 atgtggtgac ccagacaatc aagaccgccg actccgtaga agatgcccaa gtcgatcttg
     3961 tcgattgtca atatcagtat agcgatgatg aatcttttca ttttatgaac aacgagacat
     4021 tcgagcaatt tgaagtgcca atcaccgctc atgaagaccg tgctccttat ctaaaagaag
     4081 gtgaaaccta cacaatcgtc atgtgggaag gcgagcccat agatatcaag atcccatata
     4141 aaatggtttt tacggtagca gagagcgaga attatgtgcg cggcgataca gtatctgggg
     4201 caacaaaacc tgtaaggacc gagacagggc tcgtagtgcg ggtgcccctc ttcataaagc
     4261 aaggcgagaa aatccttatc aatacggaga caaacgagta cgttgagcgc gtcaac
//
LOCUS       DAOOWY010000137         4256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_20816_length_4256_cov_12.103071, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000137 DAOOWY010000000
VERSION     DAOOWY010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20816_length_4256_cov_12.103071"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PK105_08160"
     CDS             <1..649
                     /locus_tag="PK105_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU61728.1"
                     /translation="ENLSKHFGGILHIGNDATLATIAEHELCAPESDDFVCLLIDEGI
                     GSGAFINGAIHYGHNGQFGEVGHMTIVHNGLQCPCGNTGCWDLYGSELALRQALGIAR
                     GGDIPNTEELVALAASPPAWSQEAFSNFIGYLITGIVSIMNAMSPSTLVVNSNVLSAS
                     PAMFETLRAGVTARALAHTGICEIRLSSLAKDAPAIGAAMAAIEGFYEELVVKND"
     gene            complement(971..1222)
                     /locus_tag="PK105_08165"
     CDS             complement(971..1222)
                     /locus_tag="PK105_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61729.1"
                     /translation="MGNRERIWGLGRLYPALGLVPRVGFGDALRCGPAWMEAENGFCG
                     LCVRNPIVLGAFKHLVLGDKVRLAHLCLVACRRGKASAS"
     gene            complement(1233..3503)
                     /locus_tag="PK105_08170"
     CDS             complement(1233..3503)
                     /locus_tag="PK105_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61730.1"
                     /translation="MAKKVDTAPGGYKWSFMRIGGVDQVVLRNGDDIAHIPHLDQKLW
                     AALAMPKKGDGIRPETLDLLDADKDGRIRAPDICAAIDFASAGLTWLDDFFSAGDSIK
                     ISNLKTDALTEAAQKILQIVSKESVPRAQDVSLNDTMIALERFAEEKLNGDGVVSPED
                     VQSITGSEALARAVKAMVELGYARPDIRSKTGLGKEEYERFISDAKAWLEWNAKGNNA
                     ATKPLRDATDAAWSAFVVVKDKIDDWFLRSRLALLTGKREEALGEEERLKALFAGKIE
                     IGSPELLALPIAAPKTSLTLDLESAALNPGWAGALETFRRTTAALWTESASTGATSTK
                     QGTVSAADWEAIKARLAPYGAWLAEKPAGEVGALGKDMLTEIIGTPELATLPALIQED
                     ASKDTLRERMLDVQKLVLLRRDLMRILRNFVNFSDFYLDRKGIFQSGHLFLDARECEL
                     CIDVENPSAHALLASMSNVYLVYCDCTRKDGSKKTIAAGFTAGDADNLFVGRNGVFYD
                     KDGLDWDARITRIIAQPISIRQAFFSPYKLLARSIENIVQRRAATAESSTQAKLNSQA
                     ESAVATVVGEKKPGGISAAASSVATPAGTAMSTGPSGMSKRMDVGTVAAIGVALGSIG
                     TMVTNLLVGFVGMGAWLPIGILVIILLISGPSMLLAYLKLRKRNLGPILDAEGWAVNG
                     RLKINVPFGGALTHLAALPPGVERKMTDPFGEKKRPWGLYIALAVVVVLALLWVFGAV
                     DWILPIAAQFGTVIGR"
BASE COUNT         1043 a         1217 c          983 g         1013 t
ORIGIN      
        1 agagaatctc tcaaaacact ttggcggcat tttgcatata ggcaacgacg cgacactggc
       61 tacaattgcg gagcacgagc tatgcgcccc tgagtcagac gattttgtat gcctgctaat
      121 agatgaagga atcggatcag gagcttttat caacggtgcg atccactatg gacacaacgg
      181 gcagtttggc gaagtgggcc acatgaccat tgtccacaat ggacttcaat gtccgtgcgg
      241 caataccggc tgttgggatc tttatggatc cgaacttgcc ttgagacaag ccctgggcat
      301 tgcgcgaggc ggagatatac ccaacaccga ggaactagta gccctggctg catctcctcc
      361 agcatggagc caagaagcct tctcgaactt catcggatac cttatcactg gaattgtaag
      421 catcatgaac gcgatgtcac cttcgaccct cgtcgtcaac tccaatgtgc taagcgcttc
      481 tcctgcaatg ttcgagaccc ttagggcagg tgttacagcc cgggctctag cccacacagg
      541 aatctgtgag ataagactct cctcccttgc taaggatgct ccggcaattg gagccgcaat
      601 ggcggccatt gagggcttct atgaggaatt agtcgtaaaa aacgattgaa atagggctct
      661 acgatcttta attttagaga tcgaaatatt acgccgtaca aaaagactct caatagggac
      721 tatagtgatt gtcacgaatc aagatggcga ttctatgtac gagtgcatag tgattacacg
      781 acttcggctg cggccagaaa aagcggcgta caacatatta tggcaattaa ctcacaggcg
      841 gcggtgggtg caacgccaca acctagtctg gacaaggccg ccgatgctca attgcagcct
      901 atgagcgtct acggtcgaag taaagccgaa atggaacgtg tgatgcggga agtggcagcg
      961 ccagttccct tcacgatgct gaggccttcc cccgcctaca ggccacgaga caaagatgcg
     1021 caagccgcac cttgtcacca aggacaaggt gcttgaaggc ccccaacact attggattgc
     1081 ggacacaaag accacagaag ccattctcgg cttccatcca cgccggacca cagcgcaagg
     1141 catcgccgaa acctacgcgt ggtacgagtc ccagggcagg atataaccga cctaaccccc
     1201 aaattcgctc tctattgccc acgactcaat gcctagcgcc caataacggt cccaaattga
     1261 gctgcaatcg gcaaaatcca gtctactgcg ccaaaaaccc acagcagcgc cagtacgaca
     1321 accacagcga gcgcaatata cagcccccac ggacgctttt tctctccgaa ggggtcggtc
     1381 atcttccgct ctacccctgg aggcaacgct gcaagatgcg tgagcgcacc accaaacggc
     1441 acattaattt tgagcctgcc attgaccgcc caaccttccg catccagaat tggcccaaga
     1501 tttcgtttcc tcaatttgag atatgcaagc aacatcgatg gtcccgaaat caagagaata
     1561 atcacgagaa tccctatggg cagccaagcc cccatgccta caaagcccac gagcagattt
     1621 gtcaccatag tgccgatgct gcctaaggcc acgccgatgg cagctacagt ccctacatcc
     1681 atccgcttcg acatccccga tggcccagta ctcattgccg tacctgctgg cgtagccacg
     1741 ctcgaagccg cggcggatat tccacccggc tttttctcgc ccacaacagt cgcaaccgcg
     1801 ctttctgctt gactattcaa ttttgcctgg gtggatgact ctgcagtcgc cgcccgcctc
     1861 tgcactatgt tctcaatact ccttgccagc aacttgtaag gagaaaagaa cgcctgccgg
     1921 atcgagatag gctgcgctat gatccgcgta atacgcgcat cccagtcaag gccatcctta
     1981 tcgtagaaga cgccgtttcg tcccacaaaa aggttatccg catccccagc ggtgaacccc
     2041 gccgcaatcg tttttttgct cccgtccttg cgcgtgcaat cgcaatacac caagtagaca
     2101 ttcgacattg aagcaagcaa cgcatgcgcg ctcggatttt ctacatcgat acaaagttca
     2161 cattcgcgtg cgtccaggaa taggtgccct gactgaaaaa tccccttgcg gtcgaggtaa
     2221 aagtccgaga agtttacaaa gttgcgtaag attctcatca agtcccgtcg cagcagcacg
     2281 agcttttgta catcgagcat tctctctcgc agcgtatcct tggacgcatc ttcctgaata
     2341 agcgcaggaa gtgtcgcgag ctccggcgtc cctatgattt cagtcaacat atccttcccc
     2401 aaggctccta cttcacctgc aggtttctca gcgagccacg ccccataggg agccaaccga
     2461 gccttgatcg cttcccaatc tgccgcactc acagtcccct gtttggtact tgttgcccca
     2521 gtcgaagcgc tctctgtcca gagagccgct gtagtccttc taaatgtctc caaagcgcct
     2581 gcccagcccg gattgagtgc cgctgattcc agatcgagag tgaggcttgt ctttggggca
     2641 gcgatcggca aagccagcaa ttcaggcgat cctatctcga ttttccccgc gaagagcgct
     2701 ttgagccgct cctcctcgcc aagtgcttcc tctctcttcc ctgtgagcaa tgcaaggcgc
     2761 gagcgcagaa accaatcgtc gattttgtcc ttcacaacca caaaagcgct ccatgccgca
     2821 tcagttgcat ctcgaagcgg ctttgtggcc gcgttatttc ctttagcgtt ccattcgagc
     2881 caggctttgg catccgaaat aaagcgctca tactcttcct tgccaagccc cgttttactc
     2941 cgtatgtcgg gccgtgcgta tccaagctcg accatcgctt ttactgctcg cgccagcgct
     3001 tcggatcccg ttatcgactg cacgtcctcc ggagaaacta cgccgtcgcc gttgagcttt
     3061 tcttctgcaa atcgctcaag ggcaatcatc gtatcgttca gactaacatc ttgagccctt
     3121 ggcacagatt ctttggagac aatctgtagg atcttctgtg cggcttccgt aagggcgtct
     3181 gttttcaaat tcgagatctt gatggagtca ccagccgaga aaaaatcgtc cagccatgtc
     3241 agacctgcgg acgcaaaatc gatggctgcg cagatatccg gagcacgaat tctgccatcc
     3301 ttgtcggcat ccaaaagatc gagcgtttca ggtcgaatcc catcgccctt ttttggcatg
     3361 gcaagcgccg cccagagctt ttgatcgagg tgaggtatgt gcgcaatatc atcgccgttg
     3421 cgcagtacaa cctgatcgac tccaccaatc cgcatgaatg accatttata gccgccgggc
     3481 gccgtatcaa ccttcttcgc catcgcgtcc tccttccgga ctcttgagaa aaatcttttg
     3541 ttagcgaaag tataaattta tatcatattg tctgtcacta ttgtttcttc gatttttgga
     3601 ggcttttctg gtctcctgta aagagaaata tccacttaat tgagtagcag gattgtgtag
     3661 tggctgtttc atgaacatgc ctccaagggt aagcagacac tcctgtgatt ccttgtgtcc
     3721 actgtacatc gtgtctcctt cccaatgccc tgctcaccca agggatatcc ccttgttcga
     3781 tccaattgta gagtgtcttc tcacagatcc ttaaacccga aggccaggag ccttcttcat
     3841 cgagcttatt gagcaccgca tagggactat agtgatcgtc acgaatcaag gtggcgattc
     3901 tctgtacgag tgcatagtgc ttcttgtact ggttgcgatc actataaaac caagtaccaa
     3961 cagtgcgcca acaattataa ggattagact ttcgatgaaa aatgcagtaa accagtaaaa
     4021 atagcacctt gcttagaaga tgaaatatat ggctcgaatg ggaatccctc taaccatatc
     4081 accttgcgaa ccaatgaatt ggtcattcca tgatacgctg atcgttgttc ctgcgacatg
     4141 cggaaacaca aggtagagat atggcccagg aataggtgac ctgtctaatc gcattgtcaa
     4201 gtttgcgcta cattgcaatg ctgcttaacc agctttgcac aaatctgtca tgagcc
//
LOCUS       DAOOWY010000138         4201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_21198_length_4201_cov_17.168837, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000138 DAOOWY010000000
VERSION     DAOOWY010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21198_length_4201_cov_17.168837"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PK105_08175"
     CDS             <1..661
                     /locus_tag="PK105_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOU61731.1"
                     /translation="RRALLRWCLGHHNVPGMHRMAKESEAIMTCDIILCSVGGQGGLS
                     ASIILARATMASGFQVKQSEIHGMSQRGGEVLAHLRIADKEIYSPTISKGKADIMFAF
                     EPLEALRHLSWISPSNGTIVSATTAVLNISNYPDLEIVLAEIRKIPRARLIDAEAIAR
                     QAGNVRTANLVLIGAAMDLLPISPNAIEKEIIELFSKKGKSIIQANLKAFEAGRALYH
                     G"
     gene            654..3077
                     /locus_tag="PK105_08180"
     CDS             654..3077
                     /locus_tag="PK105_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOU61732.1"
                     /translation="MAETQKTESRMIAQNAKGNRLSQSAKVLRDKVRRIIARAQSEHR
                     EALTELEGMSILAAMGVQTPRYWLVQSAKEFLEKIGAEGAVDAPLQGPFSGSKAVIKV
                     MSSQVLHKTEVQGIEIVDNTAAAIADSLFRMEARFSNVPRDGFSVNEFIAFEPKLGHE
                     MIFGYRFTPDFGPIVSFGPGGIYTEYLASKFKPGSANLFLSPRVTDRAVLESLLENNV
                     IYGLLCAGLRNTTPELDEKSLIEAIQRFIDAADALSAAGISEFEVNPMVLSKTGELVA
                     LDCLVTLREFAAPAPVNASVQGSAQNPIQGLVRDSEGLPINLAQQTRPIDQIAHILEP
                     SNVAIIGVSEKTINNGRIILRNLLENGFDKSRIYVIKPGAQWIDGCKCVTDVQSLPEK
                     VDLFVVVIPALSIPSTLADIARYDKAWSVLLIPGGLEEKEGSESIVAEMNRALAEARA
                     KGKGPLINGGNCLGIRSVPGKYNTLFIPEYKLPMPKGKVEPLAVISQSGAFAITRISK
                     HPNINAKYAITIGNQMDLTVGDYLDYLAQDNEIHVFAVYSEGFKPLDGEKALKATKRI
                     SESGRSVILYRGGRTRAGAGAAASHTASIAGEYPVTWQLFSQAGAIVCESLDEFDDAI
                     TLFTLLDGKRTKGKRLAAVSNGGYECVAIADNLGEMMLSTFSERTKAELEVIFKDARI
                     AEIVDIHNPLDLTPMADDLAYERALRAVLLDPESDLAIVGIIPLTAMLNTLASNPLSH
                     SEDILRPNSLPERLARLASEIDKPFIAVVDSGPLFDPLCAELEKRGIPTFRAADRALR
                     MLERWRKSH"
     gene            complement(3163..4050)
                     /locus_tag="PK105_08185"
     CDS             complement(3163..4050)
                     /locus_tag="PK105_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU61733.1"
                     /translation="MQYDIVMMGHISKDIIIDETGNESKLYGGALLYSSISAARAGAH
                     VLAITKAAPEDFCALDIAKEQNGIEILAIQSPKTTSIRNVFLSEDHERRETSLLSSAE
                     PFLAADIPSNAETKIIDLAGLFVGELPDNLIEELAPRGAIAVDAQGLLREAQPDGRML
                     FRDWKNKHHYMPFVSYFKADAAEAEILTGLADREEAAREIAAWGKHPEGHEPEVMITY
                     NTEVIVLVDGRIYRAPYTPSNLSGRTGRGDTTFAAYLAWRLGHDPQESVRFAAALCSI
                     KMEKPGPFSGTLEDVFARM"
BASE COUNT         1090 a         1016 c         1018 g         1077 t
ORIGIN      
        1 gcgaagagct ttgctacgat ggtgtctcgg tcatcataat gttccgggaa tgcatagaat
       61 ggctaaagaa agcgaggcaa tcatgacctg cgatataatt ctctgcagcg ttgggggcca
      121 gggtggactt tctgcatcga taatcttggc ccgtgccaca atggcctctg gttttcaggt
      181 taagcaatcc gagatacatg gaatgtccca gcgcggaggt gaggtcttgg ctcacttgcg
      241 catcgccgat aaggagatct atagtcccac aatttccaag ggcaaagcgg acattatgtt
      301 tgctttcgag cccctggaag cgcttcgtca tctttcgtgg atctcgccct ctaatggtac
      361 gattgtctca gccactacag ctgtcctcaa cattagcaat taccccgatc tagaaattgt
      421 gttggcagaa atccgcaaaa tccctcgcgc tcggcttatc gatgcagaag ctatagcacg
      481 gcaagcggga aatgtgagaa ctgcaaacct tgttctaatt ggcgccgcaa tggatctcct
      541 tcctatttct ccgaatgcta tcgaaaagga gataatcgag ctattttcaa aaaagggcaa
      601 gtcgatcata caagccaatc tcaaggcttt tgaagcgggg agagcattgt accatggctg
      661 aaacacaaaa gaccgaaagt cgaatgattg ctcaaaacgc taaaggcaat cgcctgagtc
      721 agtcggcaaa agttctaaga gataaggtaa ggcgcataat agcgcgagcc cagagtgagc
      781 acagagaggc gcttaccgag ctcgaaggaa tgtctattct ggctgcaatg ggtgtacaaa
      841 cgccacggta ttggctcgtg cagtcggcaa aggagtttct agagaaaatc ggagctgagg
      901 gagcagtgga tgctcctctg caagggcctt tttctggctc caaggctgta atcaaggtta
      961 tgagttctca ggttcttcat aagaccgaag tacaaggcat cgaaattgtc gacaacacag
     1021 cagcagcaat tgccgattcg ctcttcagga tggaagcgcg ctttagtaat gtccctcgcg
     1081 atggttttag tgtcaatgag ttcatagctt tcgagccgaa gcttggccac gagatgattt
     1141 ttggttatcg ttttacgcct gatttcgggc ctatcgtgag tttcggccct ggcggtatct
     1201 ataccgagta cctcgcatcc aaattcaagc ctggctcggc gaaccttttt ctctctccaa
     1261 gagttacaga tagagctgtg ctcgaatcgc ttctagaaaa caatgtaatc tatggacttt
     1321 tgtgcgctgg cttaagaaat acaacgccag agctagacga aaagagtcta atcgaagcca
     1381 tccagcgctt tatagacgct gcagatgccc tttcagctgc aggtataagc gaatttgaag
     1441 tcaacccaat ggttctttca aaaacaggcg agctcgtcgc actggattgc cttgtcactc
     1501 taagagagtt tgcagcgcct gcacccgtga atgcctctgt gcaaggctct gctcagaatc
     1561 cgatccaggg ccttgtccga gattccgagg gcttgcctat aaatttagct cagcagactc
     1621 ggcctatcga tcaaatagcg cacattctcg agccttcgaa tgtggctata atcggcgtat
     1681 ctgaaaagac aatcaataat ggtcgtataa tcctccgcaa tctacttgaa aatggcttcg
     1741 acaaatcgag aatctatgtg ataaagcccg gtgctcaatg gatcgatggc tgcaaatgcg
     1801 ttacagatgt tcaatctctg cctgagaaag tcgatctctt tgtcgtggta attccagcgc
     1861 ttagtatccc cagcacgctt gctgacattg cgcgatacga caaagcgtgg tcggttcttt
     1921 taatccccgg aggcctagag gaaaaagaag gctcagaatc catagttgcc gagatgaatc
     1981 gcgctctggc cgaagcgcgc gccaaaggaa aaggaccatt gataaatggt ggcaactgtc
     2041 ttggcatacg ttcggttcct ggcaaataca ataccctttt catccctgaa tacaagctgc
     2101 ccatgcctaa aggcaaagta gaaccgcttg ctgtgatctc gcagtcaggt gccttcgcca
     2161 taacgcgcat ttccaagcat cccaacatca atgcaaaata cgcaataacc attggcaatc
     2221 agatggacct aacagtcgga gattatctgg attacctggc tcaagataac gagatccatg
     2281 tgtttgcggt ctattctgag gggttcaagc cgctggacgg agaaaaagcc cttaaagcca
     2341 ccaaaaggat atctgagtct ggtcgcagtg tcattctata tagagggggc agaacgcgag
     2401 ctggagctgg agcggcggcg agccacacag ccagtattgc gggcgaatat cctgttactt
     2461 ggcagctctt ttctcaagct ggcgcgatcg tttgcgaaag tctggatgaa ttcgacgatg
     2521 caattacatt atttacacta ctcgacggca agagaacaaa aggcaaaagg cttgcagctg
     2581 tctccaatgg cggctatgag tgcgttgcaa ttgccgacaa tctgggcgag atgatgttgt
     2641 ctacttttag tgaaaggaca aaggccgagc tcgaagtgat tttcaaggat gcgcgcatag
     2701 cggagatagt cgatatccac aacccactcg atctcacccc aatggcggac gaccttgcct
     2761 acgaacgggc acttagggcg gtacttctgg acccggaaag cgatcttgcc attgttggga
     2821 taatcccgct gactgccatg ttgaatacgc ttgcaagcaa tcccttatct cacagcgaag
     2881 atatccttag gcccaattcg cttcccgagc gacttgcaag gttagcgagc gaaatcgata
     2941 agcctttcat tgccgttgta gactctggtc cgctcttcga tcctctctgc gccgagctcg
     3001 aaaagcgcgg aattccaact tttcgcgctg cggacagagc cttgcgaatg ctggagcgct
     3061 ggcgaaaatc gcattgaaaa acatagagca tcaaaatgaa agaatataag ctattagagt
     3121 tgcattaagg cattaggaat tagatgcaca atcagatgcg cgctacatac gcgcaaacac
     3181 atcttcgagt gttcctgaga aaggtcctgg cttttccatt tttatggaac agagtgctgc
     3241 tgcaaatcgg accgattcct gtggatcgtg gccaagacgc caggcaagat aggcagcaaa
     3301 ggtagtgtcg cctcgtccag ttcttccgga gagattcgaa ggcgtatagg gcgcgcgata
     3361 gattcttcca tccactaaca cgatcacttc ggtattatat gtgatcataa cctcgggttc
     3421 gtggccctct gggtgtttgc cccaagctgc tatttcccgt gcggcttctt ctctgtcggc
     3481 aagacctgtc agtatctccg cttcggcggc atcggcttta aagtagctca caaaaggcat
     3541 gtaatggtgc ttgttcttcc aatccctgaa aagcatcctg ccatcgggct gagcctcacg
     3601 aagaagtcct tgcgcatcga cagcaatagc ccctctgggc gcaagttctt ctatgagatt
     3661 atcaggcaat tcgcctacaa agagacctgc cagatcgatt attttcgtct ctgcattcga
     3721 aggtatgtcc gctgccaaaa atggttctgc cgaagataaa aggcttgtct ctcttcgttc
     3781 atggtcctct gatagaaata cattgcggat cgatgtagtt ttgggactct ggatagctaa
     3841 gatttctatg ccattctgct ccttcgctat atcgagggcg caaaaatctt ctggagcagc
     3901 tttggtaatg gcgagtacat gggctccagc tcgcgccgct gaaatagatg aatacaagag
     3961 cgctccacca tagagcttac tctcattccc tgtctcgtcg attattatat ctttcgaaat
     4021 atgacccatc atcactatgt cgtattgcat acgcatctcc tcttagaaat tatttaagaa
     4081 tctcctcttt gaaattctct aactaatctc ttctttgaga tctcctttac atttgtctag
     4141 caaatttatg aaaacgtttt tagcatcctg caaacgattt cagttgcatt ttgttaaact
     4201 t
//
LOCUS       DAOOWY010000139         4199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_21218_length_4199_cov_10.472490, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000139 DAOOWY010000000
VERSION     DAOOWY010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21218_length_4199_cov_10.472490"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PK105_08190"
     CDS             <1..291
                     /locus_tag="PK105_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61734.1"
                     /translation="GRLPKATVMGFYGTHGSFGLIPWPIAMPEDISFEFPIGRSLDER
                     GTIQIDSDVSGKGGVQPEIRIPRTWQNIAAYASGKDVELEFAKQWLLNQDPR"
     gene            574..1248
                     /locus_tag="PK105_08195"
     CDS             574..1248
                     /locus_tag="PK105_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HOU61735.1"
                     /translation="MIKLIGVVIVIVGFALNLNNIAIILVAGFVTGLVGGMDLIKILE
                     TIGSSFVANRGMAIFIMIMFVTGTLERNGLREMAAKLIAKIKNATAGIVIAAYGVLRL
                     ILAAFNVSLGGTAGFVRPVVMPMAIGAVEAKHGQANEKHVEELKGMSSGMENTTWFFG
                     QVLFIGGGGALLVQSTLKNLGYETDLAELAKVEVPVAIFALIVASIIYYLKDRHLDKT
                     LYGQNQ"
     gene            1261..2205
                     /locus_tag="PK105_08200"
     CDS             1261..2205
                     /locus_tag="PK105_08200"
                     /inference="COORDINATES: protein motif:HMM:NF017931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HOU61736.1"
                     /translation="MQFFIDPKIALGVKLLEVLYIIMGLIVGYNGIKTLFEKDHKARI
                     GTAIFWISLAIILIFGHWLSKLTSGILVVIMAIPAIFGQVKPGPAAPPSKEYTQAQAE
                     KIGYKIFVPTLMMGIMAIIFALFTKISPLVGVGFGVIIAAILLMIFSKDNTPKVFLED
                     SKRMLDAVGPLSMLPMLLAALGAIFTAAGVGNVIANLVSKIIPQGNITIGIIVYALAM
                     PLFTIIMGNAFAAITVITVGIAAPFVLALGADPTIIGMLGLTCGYCGTLCTPMAANFN
                     IVPVAILDMKGKYNVIKNQLPIAAIVLVFQICYMLIAK"
     gene            2292..2900
                     /gene="pcp"
                     /locus_tag="PK105_08205"
     CDS             2292..2900
                     /gene="pcp"
                     /locus_tag="PK105_08205"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HOU61737.1"
                     /translation="MRILLTGFDPFGGEKINPAFEAVKLVQDRIADSDIIKLEIPTVI
                     GKCDKLVAQMVREKKPDVVLSIGQAGGRFDISVERVAVNIIDARIPDNEGNQPFDQQI
                     RKDGENAYFATIPVKAIAERIKSKGLPASVSYTAGTYVCNYIMYCELYMAATEFPKLR
                     CGFIHVPFSTSQAINKPTPVPTMSIENMTLAIEAAIETIIAS"
     gene            2954..3934
                     /locus_tag="PK105_08210"
     CDS             2954..3934
                     /locus_tag="PK105_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61738.1"
                     /translation="MPIPLDEGARIVIREWLRAREGERITIISDELHVAEAMAMREQA
                     QLVGASPIVILVESSSPQCGELFDDRFDAFLNTDAIVGATHNSIMTTRAIRDSVKAGA
                     RFLSIPLSTNDGIPLLQHDFIRMNTNEVNRIGLQIRSLFIDASEAHVTTDLGTDIQFS
                     IKGRLGNVFHGLCDQPGVSTSASFEFSISVVENKTEGVVILDGSMGYIGLVEEPVRIL
                     IDRGRIVSIEQNTSGRRLAKYLSDFQDERMYIIGELGIGLNKMASCAGRSYIEDECSY
                     GTFHIGIGRNISLGGQHYANGHFDLVMHRPTIKVDSHNVMKDGKIEIFEF"
BASE COUNT         1205 a          844 c          935 g         1215 t
ORIGIN      
        1 ggtcgccttc ctaaagcgac agtgatgggg ttctatggta cacacggttc tttcggtctt
       61 attccttggc caattgcgat gccagaagac atttccttcg agtttcctat aggacgctcg
      121 ctcgatgaac gcggtactat tcaaatcgat tcagatgttt ctggcaaagg cggagtgcag
      181 cccgagattc gcatcccacg aacttggcag aatattgctg catatgcgtc aggcaaggat
      241 gtcgagcttg aattcgccaa gcaatggctt ctaaatcaag atcctagata ggcgcgctta
      301 gattatagaa atcggcccaa aataatcttt atagatacac tctcaagcca ttgctcactg
      361 caaagccatt gagatacgac tggccgctat tggtctttgc tccaacgcca ttcgatccta
      421 atttctccat agagtcgagt gtcttagttt gccatgaagc taaattatat ttaatcactt
      481 tttgatgggt tcttaaaatg aatatttaca acggctttta tgtctcatat aatccattaa
      541 atcaaacagc ttatcttgca taaggaggca aatatgataa agctgatagg agttgtaatt
      601 gtcattgttg gcttcgctct gaatctcaac aatatcgcca ttattcttgt tgcaggattt
      661 gtgactggtc tcgttggtgg aatggatttg ataaaaatct tggaaacaat cggaagcagc
      721 ttcgtagcca atcgaggaat ggcaatattc atcatgataa tgttcgtgac aggcacttta
      781 gagcgaaatg gattacgaga aatggccgcc aagcttatcg caaaaatcaa aaatgctacc
      841 gctggcatag ttattgctgc ctatggtgtt ctacggctaa ttcttgcagc attcaatgta
      901 agcctaggag gtacagctgg atttgttagg ccagttgtca tgcctatggc tataggagct
      961 gtcgaggcga aacatggcca ggcaaatgaa aaacatgtag aggaacttaa aggcatgagt
     1021 tctggtatgg agaataccac ctggtttttt ggacaagtcc ttttcattgg tggtggaggc
     1081 gctctccttg ttcagtctac gctgaagaat ctcggatatg agactgactt agcagagctt
     1141 gcaaaggtgg aggtacctgt tgctattttt gcacttatcg tagcatcgat tatttattac
     1201 ctaaaagata ggcaccttga taagactctt tatggccaga atcaataagg aggcgataaa
     1261 atgcaatttt tcatagatcc taagattgcc cttggggtaa agttgcttga ggttctctat
     1321 ataataatgg gactaatagt aggatataat ggaatcaaaa cccttttcga gaaagatcat
     1381 aaagcaagga ttggtactgc aatattctgg atttctttgg caataatatt gatatttggt
     1441 cattggttgt cgaagctaac tagcggcata ctcgtagtta tcatggccat ccctgcaata
     1501 tttggtcagg taaaacctgg cccagctgcc ccaccaagca aagaatatac ccaagctcaa
     1561 gcagagaaaa taggttacaa gatatttgtt ccgaccctca tgatgggaat aatggctatt
     1621 atctttgccc ttttcacaaa aatcagccca ttggtaggcg taggatttgg tgtcattatt
     1681 gcagcgatac ttctcatgat tttctcaaag gataatactc caaaggtttt tcttgaggat
     1741 tccaaacgca tgcttgatgc agttggtcca ctttctatgt tgccaatgct tcttgctgcc
     1801 cttggagcta ttttcactgc cgctggagtt ggtaatgtca tagctaactt ggtaagtaag
     1861 attattccac aagggaatat tactattggc attattgtct atgctttggc tatgccactt
     1921 tttacaatca taatgggcaa tgcttttgct gctattacag ttatcacggt cggtattgct
     1981 gcgccattcg ttcttgctct tggagctgat ccaactatta ttggaatgct tggattgaca
     2041 tgtggctatt gtggtaccct ttgcacgcca atggcggcta acttcaatat agtacctgtt
     2101 gccatccttg acatgaaagg caaatacaat gttatcaaga accagcttcc cattgctgcg
     2161 atagtattgg tgtttcagat ctgctatatg ctaatagcaa agtagatcaa aaagaaaact
     2221 attgcttttt tgttaccgta gaagccctaa aaaccttcta cggtttctcg atgagaaagg
     2281 aggtgcgtgt aatgcgaata ttattgacag gttttgatcc attcggagga gagaagataa
     2341 atccggcttt cgaagctgta aaacttgttc aagacagaat tgcagattct gatatcataa
     2401 agctcgagat ccccactgtc attggcaagt gcgacaagct cgtagcccag atggtcagag
     2461 aaaaaaaacc tgatgtagtg ctctcgattg gtcaagcagg aggccgattc gatatttcgg
     2521 tagaacgtgt tgcagtcaat attatcgatg cgcgtatacc tgacaatgaa ggcaaccagc
     2581 ctttcgatca acaaattcga aaagatggtg aaaatgcata tttcgccacg atacctgtaa
     2641 aagcgatagc agaacgcatc aagtcgaaag gtctacccgc aagtgtatcc tatacggcag
     2701 gcacttatgt ctgcaattac atcatgtact gcgagctcta catggcagca actgagttcc
     2761 caaaattgcg ctgcggcttt attcatgttc ctttttctac atctcaggcg ataaataagc
     2821 ctactccagt accgaccatg tctatcgaga atatgacttt ggcaattgaa gctgcaattg
     2881 aaacaattat tgcctcatga accactctag tcataaatag acataatcat atataagcag
     2941 gacagcaaat aacatgccaa ttccattaga tgaaggagcg agaattgtca tccgtgaatg
     3001 gcttcgagcc cgagaaggcg agaggataac aataatatcc gatgagctgc atgttgcgga
     3061 agctatggct atgcgtgaac aagcccaatt agtaggagca tcacctatag taatcctcgt
     3121 ggaaagctcg agtccccaat gtggcgagct ttttgacgat cgatttgatg cttttctcaa
     3181 cactgatgca attgttggcg caacacacaa ttcaatcatg acgactcggg caatacgcga
     3241 ctcagtcaaa gcgggtgcgc gtttcctatc tatacctttg tccacgaatg atggaattcc
     3301 tttgctacag cacgatttta ttaggatgaa tacaaatgaa gtcaatagga taggcttgca
     3361 aattcgaagc ttattcatcg atgcatctga ggctcacgtg actacggatc tgggtactga
     3421 tatacaattt tcaataaaag gccgtctagg aaatgtcttt catggcttat gtgatcaacc
     3481 aggagtttct acttctgcaa gtttcgaatt ctccatctct gtggtcgaga ataaaacaga
     3541 aggtgtggtt attcttgatg gctcaatggg atacataggt ttggtggagg aaccagtaag
     3601 gatacttatt gatcgaggca gaattgttag tattgaacag aatacttctg gtcgcaggct
     3661 cgcaaaatat ctgtctgact tccaagacga aagaatgtac ataatcggag aacttggaat
     3721 agggctcaat aagatggcaa gctgcgcagg ccgttcctat atcgaagatg aatgctctta
     3781 tggaactttt cacattggca ttggtcgcaa tatatcgctc ggtgggcagc attatgctaa
     3841 tggccatttc gaccttgtta tgcatagacc aacaatcaaa gtcgattccc acaatgttat
     3901 gaaggatgga aagatagaga tttttgaatt ttgaaaatag attggtctta tattgatgga
     3961 gaggcaaatc gctggatctt gggattatcg aagagcgagg attaagcctt gtcgcaaaga
     4021 agtccctctc ctcgctcaga ttatagaaat aggccctaaa taatctctat agatataccc
     4081 ttaagcctgt gctcgccgaa aagccattga gatacgactg gccgctattg ctctttgctc
     4141 caacgccatt cgtaccaagc tcgataaaat aagaagatga tagcggaccg ctggccttc
//
LOCUS       DAOOWY010000140         4048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_22287_length_4048_cov_12.295016, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000140 DAOOWY010000000
VERSION     DAOOWY010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22287_length_4048_cov_12.295016"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..2338)
                     /locus_tag="PK105_08215"
     CDS             complement(317..2338)
                     /locus_tag="PK105_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61739.1"
                     /translation="MRQKALTSLILLIFLISVEVVAGGLRLDFSAHSTGGDENSMSTY
                     SYIKEPRIEETGHTRGLKSGSFNYFKDGSKIEFNETLMLDYGNATNISNSSLSHTLNL
                     TFKGDLGISEMYGSGLYPNNRGLAAWKKIRYETSNYLKTKDGEKIRDFKANEIWVNSA
                     ANMYTRDEESVYSFDYNANAKDAVLETRDYAGWSNRTGSRRIDVEHSTMMTGDFEVTN
                     IFQETMFPGPDVLGDWLPCCFNSTIPSLRQPENPWPSQVIISTLQEYPFQPKIACKGG
                     FKEECGDWQQNETTKEWEKTCKLNPSAIGQSTAEGFKEECGEWQQNKDTKEWEKTCKL
                     NPSSIEGQICKDGFKDCPESGCPGYECIKTFNDDQYDYAPEVEVFGKKLNAIQAIYSY
                     GNLPENESYVLYPVSVRNIGINTLNDVKLKGSLPNEFDIICIYVMNASGSHINLTQGI
                     EWDSTRKSFTYGIDDLTRSAQRKIYLLVTATGNADPDEAKFSVTGNTTEDLIVKDDAS
                     WLPIDPIELDYAHNVIKLQGSEEPVNPIELNKGLVQVNDTTWILALSVKNKDDTILLK
                     DVNLEVDLSGLNTPMLIYEVIDPETVNLIKMPADKILKLHDIDPGKTNQIHIVIPENI
                     TQKFTTIDPSVSGSLNGKQFPPIWGGIFVPAGEKIKELLNKAEDKMRGG"
     gene            complement(2360..3202)
                     /locus_tag="PK105_08220"
     CDS             complement(2360..3202)
                     /locus_tag="PK105_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61740.1"
                     /translation="MNTKKSDFTGIFGSILRIPEISTKIRGTTGIQKSLLEPDENSHT
                     IDDVINEMRGLPRTQYIKDYWDCEDRALFAISRARCKFPCMPIGLAIGYCTSAIEGLH
                     ALVVVWSKDLTKGEFYDPELRETLGFNPEVIIPFPCDGTKRPEGIPYASNLPFLPRGG
                     AFVLDSTYDFSKEKIATAREFLENKGPEECEESNSACSKKTFRKCYRFSDRVLSWYIR
                     SKAEKEMLGAPIGVAFGKWKNQYMGVLLLWNDPSLRPEYWRIDNIRMRSDDARYFRPE
                     IIIA"
     gene            complement(3705..>4048)
                     /locus_tag="PK105_08225"
     CDS             complement(3705..>4048)
                     /locus_tag="PK105_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU61741.1"
                     /translation="MISDRDLIKASVIEDLVEKTDMSADGGEDAWMWDRFVQTINKYY
                     TVSRISLKNILVREAMLPAITAFKKDEVSQCAAVMHKKRIDQMPVITAGGRLTGMLKD
                     RDILRGMVEGR"
BASE COUNT         1035 a          933 c          756 g         1324 t
ORIGIN      
        1 gttgttattg cgagagggcg gtccatgcgt cggcggagga ttgtccggag aggattgatg
       61 tgcctaaacc gcatgaagtc tcttgctcga ctcgaaaaga taggcttctc ctagttcgat
      121 ttgtcctttc cagatacgat tctgatggag attaagtatg aagccaattc aaaagagcca
      181 tctggaaaaa gcagcatgta gccatgccga aaaagtagac ggaatttgtt ttaaaatttt
      241 aatacccgag atcccaaccg ccgggcgata ggcgagagac ctagggagtg gaaataaatt
      301 aaataaaaaa aataaactaa ccccccctca tcttatcttc cgccttgttc aataattctt
      361 tgattttctc cccagcgggc acaaatatac ccccccatat gggaggaaac tgttttccat
      421 ttagtgatcc acttactgat ggatcaatgg ttgtgaattt ctgggtaata ttctcaggaa
      481 tcactatatg gatctgattt gtcttgcctg gatctatatc atgcaatttt aggatcttat
      541 cagctggcat tttgataaga tttactgttt ctggatcgat aacctcatag attaacatag
      601 gggtgttcaa gcctgataaa tcaacctcca agtttacatc tttcagtaaa attgtatcat
      661 ccttattctt aacagataaa gcgagaatcc atgttgtgtc gtttacctgg actagtcctt
      721 tattcaactc tattggattc acgggctcct ctgaaccctg caatttaatt acattatgcg
      781 cataatccaa ctctattgga tcgataggaa gccagcttgc atcgtcctta acaattaagt
      841 cttcggtggt gtttcctgtt accgaaaatt tggcttcatc tggatctgcg tttccggtag
      901 ctgtgacgag tagatagatc ttccgctgtg cacttctggt caggtcatca attccatagg
      961 tgaaagattt gcgagttgaa tcccactcga tcccttgggt taaatttatg tggctaccac
     1021 ttgcattcat cacatatata cagatgatat caaattcgtt cggcaagctt ccctttaatt
     1081 tcacatcgtt tagggtatta atgccgatgt ttctaacgga taccggatag agaacataac
     1141 tttcgttttc cggcagattg ccgtagctgt atatagcttg aatagcatta agcttctttc
     1201 cgaaaacctc gacttctggt gcataatcat attgatcatc gttgaatgtc tttatgcatt
     1261 catagccagg acatccgctt tccgggcaat ccttgaatcc atctttgcat atctgtccct
     1321 ctatcgaaga tggatttaat ttgcatgtct tctcccattc ttttgtatcc ttattctgtt
     1381 gccactcgcc acattcttcc ttgaacccct ccgcggtgct ctgccctatt gcggaaggat
     1441 tcaatttgca tgtcttctcc cattcttttg tagtctcatt ctgctgccag tccccgcatt
     1501 cttccttaaa tccccctttg caggctattt tgggctggaa aggatattcc tgtaaggtgc
     1561 tgattattac ttgtgagggc catggatttt ctggttgcct gagagatggt attgtgctat
     1621 tgaaacagca tgggagccaa tctcccaata catccggtcc agggaacata gtctcctgga
     1681 atatattcgt aacctcgaag tctccagtca tcattgtgga gtgctcaaca tcaatccttc
     1741 ttgaaccggt ccggttgctc catcctgcat aatcccttgt ttccagaact gcatctttag
     1801 cattggcatt gtaatcgaat gaatatacag attcttcgtc acgcgtatac atatttgcgg
     1861 ctgaattgac ccaaatttcg ttggctttga aatctcgtat tttctctccg tctttcgtct
     1921 taaggtagtt gcttgtttcg tatcgaatct tcttccatgc agccaaacct ctattattgg
     1981 gatataggcc gcttccgtac atctctgata ttcctaaatc ccctttgaaa gtcagattga
     2041 gggtgtggct cagagaagaa ttactgatgt tggttgcatt gccataatct agcatgagag
     2101 tttcattaaa ttctattttc gatccatctt tgaaataatt aaatgatcca cttttaagtc
     2161 ccctggtatg gccggtctcc tctatcctgg gctcctttat gtaagaatag gtgctcatcg
     2221 aattctcgtc gcccccggtg ctatgagcgg agaagtcgag ccttaagccg cctgccacca
     2281 cctctacaga aattaaaaaa attaataata ttaatgatgt taatgctttt tgtctcatat
     2341 tcgccccacc gaatttgttt cacgcaatga tgatttcagg cctgaaatac ctggcatcat
     2401 cgcttctcat tcttatattg tctatccgcc aatattcggg cctcaagctt ggatcattcc
     2461 acaggagcaa gactcccatg tattgattct tccacttgcc aaaggcaacg ccaattggcg
     2521 ctccaagcat ttctttctct gctttgctgc ggatatacca agacaagact ctgtcggaaa
     2581 agcgatagca tttcctgaaa gtcttttttg agcatgccga attactttct tcacattcct
     2641 caggtccttt attttcgaga aattctctcg ctgtagctat cttttctttg gaaaaatcat
     2701 atgtgctgtc caaaacaaat gctccacctc ttggcagaaa tggtaggttg cttgcatagg
     2761 gtattccttc tggtcgcttt gtcccatcgc atgggaaagg aattatcacc tcaggattaa
     2821 aacccaaagt ctctctcagc tccggatcat aaaattcgcc cttggtgagg tcttttgacc
     2881 aaacaacaac aagagcgtgt agtccttcaa ttgctgatgt acaatagcct atggcaagcc
     2941 ctatcggcat gcagggaaat ttgcatctgg ctctggatat tgcaaaaaga gctctatctt
     3001 cgcaatccca ataatctttg atgtattgcg ttcttggcag acctctcatc tcatttatga
     3061 catcatcaat tgtatgactg ttttcatcag gctctaagag gctcttctgg attccagtag
     3121 ttcctcttat ctttgttgaa atctctggga tccttaaaat agatccgaag attcccgtaa
     3181 aatcgctctt ttttgtattc atagtattaa tcgtgctctt ttactattag tattttttgt
     3241 ttctataagc agtcttcttt taaaaaagat tatcttactt tgatataaat ttatttcagc
     3301 taataatatt taaaatattt caataaatta tttagaaatt aaatagaagg aaatgaattg
     3361 tgcccaataa taccgctcag tacaccaatg catgcagacg acccttccaa gtgcagttaa
     3421 ttaaacgtga aaccgatctg aaccggctcg aaactatttt gatcgtcgat gccctcattc
     3481 cgttcgttgt aagctgcaag atttcatttg acagtattaa acatattcta ctattagttt
     3541 cttatagata acttcttcca gaaatatttt tctaaaaaat atttagcttt ttatagcttt
     3601 gacgaaggtc tgaatttcgt tgaaaaatgc aataaaattc caaattttag ataaaaagat
     3661 gggtaatttg caggtcaaga tatcttgacc catttagctc ttatttatct cccctccacc
     3721 atccccctca atatatccct gtccttcagc atcccggtca gcctcccccc cgccgtgatc
     3781 acgggcatct gatcgatcct tttcttatgc atcaccgcgg cacactggct cacctcatct
     3841 ttcttgaatg cggttatagc cgggagcatt gcctcgcgaa ccaggatatt cttcaggcta
     3901 atccgggaga cggtatagta cttattgatg gtctgcacaa aacggtccca catccaggca
     3961 tcctctcccc cgtctgcaga catatctgtc ttttccacga gatcctcgat gacgctggcc
     4021 ttgatcaggt cccggtcgga tatcattc
//
LOCUS       DAOOWY010000141         4020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_22509_length_4020_cov_13.148802, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000141 DAOOWY010000000
VERSION     DAOOWY010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22509_length_4020_cov_13.148802"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2350)
                     /locus_tag="PK105_08230"
     CDS             complement(<1..2350)
                     /locus_tag="PK105_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU61742.1"
                     /translation="MSFCSNPILKALILSLILATAAILPLSAVDIDSPLSESSEGAFS
                     GSDPNAIQSPQISEPDAEKAPESKPPEILEPEPSKKLEPEPALPPSLFLPILTEPFDP
                     SGFFFAAEKIRTQASEQKTNLTEDQQKLIEWMLEIAFRFAKNPAGQEAGKSLIKVLSN
                     QKKYGEAAAIAREWIQVFGPNWNIYRSLYDCLMAQNAPQDALALVAEISKVVPAMAKS
                     HPSELSWMEYNARAAMQDYTWASNGPPFIRTRTPDNYITKIYRLYAAAPNVPQNLAYL
                     ALFRAAATEKNYTEALANALPILSTFSATDALRQWITELGRSFYGAGAWKDGIAYFSQ
                     ALNLPVIDSEESNGGRNNQGNADTEADAEIDAEVDAEADANAQTEAEPRAEIAPQEGA
                     PREAASKEVDGSEHIPDSKLTIPPLDRERISSENYWVMAFHLARSYQGLGQNQTAASL
                     FIELVPLSFSEADSDSALWYWLDITMKSIVTTSMILDDLGEEDANALHAERALEISAL
                     SQAAALWKSPSYFEDIASEYMRRLLREGAWNDVVRLCTLMSQKLTGIMRGPLLYISGR
                     LYETTRANVDLAMNSPNLYYETLLRDSGIEEHYRTLAAWRLGIEPPLLANLPVLGADF
                     DLESSIVSLCQDLMDSLTEDSNLKDVLDFIDQSLDYGLETLASEQITALSPYNTESLL
                     WLAMNFIQHEQYYPALRIGREALGRKSFDRPELAYALVYPRAWPEHFSQITEPQGIAE
                     PLAYAIVRSESLFNQRAVSRSGAVGLSQLLPSTAAETAKGLKM"
     gene            complement(2365..2724)
                     /locus_tag="PK105_08235"
     CDS             complement(2365..2724)
                     /locus_tag="PK105_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61743.1"
                     /translation="MKPEIKTINDLLDALNLISTRAWTPKELSRAIEQALSTLPRSVI
                     EIMIGLAGEKIRSSPLRGPLVIATFSSVLLKQYDGTPLTLSEWRELRDLVSDCADELD
                     MEIVTYVMSLIMDYGAL"
     gene            complement(2788..3456)
                     /locus_tag="PK105_08240"
     CDS             complement(2788..3456)
                     /locus_tag="PK105_08240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOU61744.1"
                     /translation="MINLSPKRIPAYGTILFVYATVWADIILAIPEPTAFSAAWFILS
                     VLKNCIRIGFMLYISSQLPIFANNEWKRALLRLPTRREIARALSVTFLSLGVAAIGAI
                     AAWFCALSNPVFEFIPQKSILSLLPFLLLSSVSVGYSEELFFRFLLIDALTEADTALN
                     SAAIVSILIFSLSHYAQGIFGIAFAGVLGALYTSLRFRGYGIHSLALGHALYDAAILA
                     LALS"
     gene            3551..>4020
                     /locus_tag="PK105_08245"
     CDS             3551..>4020
                     /locus_tag="PK105_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61745.1"
                     /translation="MRGFYCSRWFRCAKVRTRIIRRYCLQKVPAGCFAAVGLWIGLGL
                     NGLGLDSLEIGPYFEIGLGSLQLIFAIILNAVALTWLEFIGTIGKCSGGKQSFIIISA
                     PSNLSTRSNKRDVLLWSALSLGLALSAGVLRFQDQEASFIVPAEPATAASAVAS"
BASE COUNT         1048 a          893 c         1037 g         1042 t
ORIGIN      
        1 tcattttgag ccctttggcg gtctccgcgg ctgtgctggg caagagttgg ctcaatccta
       61 ccgctccaga acgcgagacg gcgcgctgat tgaacagact ctctgagcgc acaatagcat
      121 aagcgagtgg ctccgcaatt ccttgcggct cagtgatttg gctgaaatgc tctggccagg
      181 cacgcgggta gaccagggca taagcgagtt ccggtctatc gaaagatttg cgacctagag
      241 cctctctacc aattcgcaac gccggataat attgttcatg ctggatgaaa ttcatggcaa
      301 gccacaaaag cgactctgtg ttataaggag acagtgcggt gatctgttcc gaggctaagg
      361 tttcgaggcc ataatccagg gattggtcta tgaaatcgag cacatccttg agattgctat
      421 cttcggtaag tgagtccatc aggtcttggc aaaggcttac aattgaagat tcgagatcga
      481 agtcggctcc aagcacgggg agattggcaa gtagcggcgg ctcgatgccc aatcgccatg
      541 ccgctaatgt tcgataatgc tcttcaatgc cgctatctcg aaggagtgtc tcgtagtaga
      601 gatttggact attcatggcc agatcgacat tggctctggt tgtttcgtag agtcttcctg
      661 agatatagag caagggtccg cgcataatgc cggtcaattt ctggctcatg agagtacaga
      721 ggcgaactac atcattccaa gccccttctc gaagtaatcg gcgcatgtat tccgatgcga
      781 tatcctcgaa ataggagggg cttttccaaa gagcagcggc ttgtgaaagc gcggagattt
      841 caagggcgcg ttcagcatgc agggcatttg cgtcttcctc tccgagatca tcgagtatca
      901 tgcttgttgt cacaatagat ttcattgtga tatctagcca ataccatagc gcagagtccg
      961 aatcagcttc ggaaaaagag agtggaacga gttcgataaa gaggctcgct gcagtctgat
     1021 tttgtcccag cccttgatac gatcttgcca ggtgaaaggc catgacccag tagttctcgg
     1081 atgaaattcg ttctcggtcg agcgggggaa ttgtgagctt ggagtctggt atatgctctg
     1141 aaccatcgac ttcttttgag gcggcttctc tgggcgcgcc ttcttgggga gcgatctcgg
     1201 ctctgggctc ggcctcagtt tgggcattag catctgcttc agcatctact tcagcatcga
     1261 tctcggcatc tgcctcagta tctgcgtttc cttgattatt tcttccgcca ttggattctt
     1321 cactgtctat tacgggaaga ttcagcgcct gggaaaaata ggcaatacca tctttccagg
     1381 ctcctgcgcc ataaaaactg cgcccaagtt cggttatcca ctgtcgcaag gcgtccgttg
     1441 cggagaaagt agacagaata ggcaaggcat tggccagggc ttcggtataa ttcttttctg
     1501 tagccgctgc tcgaaataag gcaagatatg ccagattctg tggcacattt ggcgctgctg
     1561 cgtatagcct gtagatctta gtaatatagt tatcaggtgt tcgtgttctt atgaaaggag
     1621 gaccattgga agcccaagta taatcctgca ttgctgcgcg ggcgttgtat tccatccagg
     1681 aaagctcaga gggatggctc tttgccatag ctggcacaac tttggatatt tcagcaacaa
     1741 gcgcgagggc atcttgcggc gcattctggg ccattaggca atcgtaaagg ctgcgataga
     1801 tattccagtt ggggccgaat acttgaatcc actctcttgc gatagcagcg gcttcgccat
     1861 acttcttttg attggacaaa accttgatca aggattttcc agcctcttga cctgcggggt
     1921 tctttgcgaa tcgaaatgcg atctccaaca tccattctat gagtttttgc tgatcttcag
     1981 tgaggttagt tttctgctcc gaagcttgtg tacgaatttt ttcggccgca aagaaaaaac
     2041 ccgatggatc aaagggttcg gtcaatatgg gtagaaaaag agatggaggc aaggccggtt
     2101 caggttcaag tttttttgaa ggctcaggct ccaaaatctc tggaggctta gattccggag
     2161 ccttttcggc gtcgggctcg gaaatctgag gagactggat agcattggga tcactaccag
     2221 aaaaagctcc ttccgagctc tcggaaaggg gcgaatctat atcaacagca cttagaggga
     2281 gtatggccgc ggttgccaaa atcaaagaaa gaatcagagc cttaagaatg gggttagaac
     2341 aaaatgacaa atctctaatc ctcttcacag cgcgccatag tccattatga gtgacattac
     2401 gtaggtaacg atttccatat ctagctcatc agcacaatcg gaaaccaggt cgcgcaactc
     2461 tcgccactca gagagggtca agggtgttcc atcatattgc ttgagaagca cgctgctaaa
     2521 agtcgctata accagaggtc cacgcaaggg gctggagcga attttttctc ctgctagtcc
     2581 aatcattatc tcgattaccg atctcgggag agtggacaaa gcctgttcta tcgctctcga
     2641 gagttccttc ggtgtccatg cgcgtgtcga tataagattg agggcgtcta ggaggtcatt
     2701 gatggtttta atctctggtt tcatggcaaa tctcctgtta gtcatcaata ttatcgacaa
     2761 gcctatacat gggctcaacc cggacaatca agaaagagcc aatgccagga ttgcagcatc
     2821 atatagcgca tgtccgagcg caagagagtg tataccataa cctcgaaatc gcaggcttgt
     2881 gtatagagcg cccagtactc cggcgaatgc aatcccgaaa attccttgag cataatgaga
     2941 aagcgaaaag atcagtattg agacgatagc cgcggaattt agggctgtat cggcttctgt
     3001 aagcgcatcg atgagtaaaa aacggaagaa taattcctca ctgtaaccca ctgaaacgct
     3061 cgaaagcagc aaaaatggaa gcaaagataa tatcgacttt tgtggtatga attcgaagac
     3121 aggattggaa agggcacaga accatgcagc tatggctcct atggccgcaa ctccaaggct
     3181 aaggaatgtc acagaaagag ccctggctat ttctcgtcta gttgggagac gtaaaagggc
     3241 tctcttccat tcattgtttg caaaaatcgg cagttgagat gaaatataaa gcataaatcc
     3301 aattcggata caatttttta gtacgctgag gataaaccaa gcagccgaaa atgctgttgg
     3361 ttcaggtata gcaagaataa tatcggccca gacggtggca tatacaaaga gaatcgttcc
     3421 ataggcggga attcgcttgg gggataggtt gatcatgata ttgcttagac attactacga
     3481 atctgattct ctgtcatcgc attttgaagc caaaaatgaa tttctgtgcg tattacaaaa
     3541 gagagagata atgcgcggat tttattgctc aagatggttt agatgcgcta aagtccgaac
     3601 aaggattatt cgacgatatt gcttgcaaaa ggtaccggcc ggttgttttg cagcggtcgg
     3661 attgtggatt gggcttggct tgaatggcct cggactggat agccttgaaa ttgggccgta
     3721 tttcgagatt ggattaggca gtctacagct aatttttgct ataatcttaa atgcagttgc
     3781 gctgacctgg ttggaattca tcggcacaat agggaaatgc tcaggaggaa aacagagctt
     3841 tataatcatt tcagctccat ctaatctatc tactagaagc aacaaaagag acgtattgct
     3901 atggagcgcc ttgtctttag gcctcgcgct cagcgcaggc gtgcttcgat ttcaagatca
     3961 ggaagcgagt tttatagtcc cagcggaacc ggcaacagcg gcctcggcag tggcgtccca
//
LOCUS       DAOOWY010000142         4005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_22624_length_4005_cov_22.643038, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000142 DAOOWY010000000
VERSION     DAOOWY010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22624_length_4005_cov_22.643038"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PK105_08250"
     CDS             <1..1112
                     /locus_tag="PK105_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOU61746.1"
                     /translation="SMGVFAGIAPWNFPAMIPMGWMAPLCIATGNTMVIKAASFCPET
                     SMRIMELWAEAGLPAGVLNLITCSRQEAEIFLRHPDVKGITFVGSTSVGRHIYEVAAA
                     NGKRVQCLTEAKNHALVLKDAALERTARGIINSAFGCAGERCMALPVIVVEEEIADEL
                     VGLLKKQLKDLKVGPAYDKTSQMGPVVNADHKASVVKWIEKGIEEGAELVLDGRNIVV
                     EGYENGFYLGPTILDHVKPEMSIGWKEVFGPVLCVKRVKDFEEGLELMNSSEFGNGSV
                     IYTQSGYYAREFAKRTHGGQVGINVGIPVPLSVFGFTGHKNSFFGDLHTMGTDGVRFY
                     TELKSVTSHWFSEEEAREGKVLNSWDGTISFPDKK"
     gene            complement(1236..2093)
                     /gene="panB"
                     /locus_tag="PK105_08255"
     CDS             complement(1236..2093)
                     /gene="panB"
                     /locus_tag="PK105_08255"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOU61747.1"
                     /translation="MPDEVQKLSIPDLVSMKREGKKISMITAYSYPQGLLVDEAGIDI
                     ILVGDSLGMVELGYNGTTPVTMDEMLAHIKPVTRAVKRAHVVGDMPFMSYNVSKEQAI
                     TNAGILYKEGACDSIKLEGGQEMANTIEAIVRAGIPTFGHIGLTPQTAGMLGGFKVQG
                     KSLDAAKKLIDDAIAVDEAGAFAIVLESIPRQIAKIISQKVKAITIGIGAGMECDGQV
                     LVFHDILGLFKRFTPKFVKVYANAWDYELKAIKDYIDEVREQKFPSDEFTYTMKEDVL
                     NELKKYAGI"
     gene            2443..3720
                     /locus_tag="PK105_08260"
     CDS             2443..3720
                     /locus_tag="PK105_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOU61748.1"
                     /translation="MNTVEKYKNYVNTSFVKAIEPVIVTNASGSKITSEDGKVYTDLY
                     SGISVVNAGHCNPEVAEAAKAQIDKLIHCGTYVYYAPTVADFAEKLAEVAPGRLQKTF
                     FGNGGAEANECGMRMAKQYTKKQEFIALQGSFHGRSLATLSITGNKARKKGGGPYIPG
                     VAFNPAPYCYRCGFDMSYPSCDLYCAKYLERTIQYDTSDNVAAFVAETIMGEGGIIVP
                     PKEFFKEIKKILDQYGILLFLDEVQAGFGRSGKLFAIEHYDVEPDIMTMAKGIAAGFP
                     LSACIARPEIADSFRPGDHLSTFGGNPVSCAAAIANLNYMLREKLPEKAMEKGKIFIE
                     ELKEIQKKTPLIGDVRGSGLMIGVELVRDKRKTPAAEEAAKIKTLMREKGFLIGLGGT
                     FANVLRLQPPLVISSEELSSAANALSECFRELV"
     gene            3717..>4005
                     /locus_tag="PK105_08265"
     CDS             3717..>4005
                     /locus_tag="PK105_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HOU61749.1"
                     /translation="MKISVIGSGAMGSLFGGKLAIAGENVVLYDVYRDHIDTVNKEGL
                     SIEDAETGKITVVYPQASSDPESVKNSDVLLIFVKSTNTESVANQFKSFAAP"
BASE COUNT         1127 a          732 c          990 g         1156 t
ORIGIN      
        1 agagcatggg ggtatttgcg ggcatagctc catggaattt tcctgcgatg ataccaatgg
       61 ggtggatggc acctctatgc attgctacag ggaatacgat ggtaataaag gctgctagtt
      121 tttgtcctga aacttctatg aggataatgg agctatgggc ggaggcaggg ttacctgctg
      181 gggtattgaa tttgattacg tgttcgcggc aggaagctga gatatttctg cggcatccag
      241 atgttaaagg gataactttt gtaggttcta cgagtgtagg gaggcacatc tatgaagtag
      301 cagcggctaa tgggaagcga gtgcaatgtc ttaccgaagc aaagaaccat gctctagtat
      361 tgaaggatgc ggccttagag aggacggctc gagggataat caattcggca tttgggtgtg
      421 caggtgagcg ttgtatggca ttgccagtga tagtggtgga ggaagagata gcggacgagc
      481 tagtagggct tcttaagaag caattgaagg atctcaaggt agggccggcg tacgacaaga
      541 cgagccagat ggggccagta gtaaatgcgg atcacaaggc atcggtagtg aaatggatag
      601 agaaaggaat agaggaagga gcggagctgg tactggatgg tcgaaatatc gtagtagaag
      661 gctatgagaa tggcttttat ctagggccga cgatattgga tcatgttaag ccagagatga
      721 gcataggatg gaaagaagtt tttgggccag tactctgtgt gaagcgagtg aaggatttcg
      781 aggaaggcct agagctcatg aattcgagcg aatttgggaa tggttcggtg atatatacgc
      841 agagtggata ttatgccagg gaatttgcaa agaggacgca tggagggcag gtaggtatca
      901 acgtaggcat tccagtacca ttgagtgtat ttggttttac tgggcataag aattcatttt
      961 ttggggatct gcataccatg gggacagatg gagtgagatt ctacacggag ttgaagagcg
     1021 ttacttctca ctggttctct gaagaggaag ctcgcgaagg caaggttctc aacagttggg
     1081 atggaacaat atctttccca gataagaaat aaaagataaa aatccttttg tccataattc
     1141 aaaaatgcct gtagcgctca tcgagcttta caggcatttc caatttttaa attatcctaa
     1201 ttctcgattg tcttgaagag atttcttttt tactgttaga ttcccgcata ttttttgagt
     1261 tcgttgagca cgtcttcttt cattgtatat gtaaattcat cagatgggaa tttttgttct
     1321 cttacttcat caatataatc ttttatagcc ttcagttcgt aatcccaagc atttgcatac
     1381 actttgacaa actttggtgt gaatcttttg aaaagtccaa ggatatcatg gaagacgagc
     1441 acttggccgt cgcattccat gccagcgcca atacctatgg ttatagcctt aactttctgg
     1501 gagataattt ttgcaatttg tcgaggaatg gattctaaga ctatagcgaa tgcgccagcc
     1561 tcatccacgg ctattgcatc gtctatcagc tttttagcag catcgaggga cttgccttgc
     1621 actttgaaac cgccgagcat gcctgcggtt tgaggagtaa gcccaatgtg accaaaagtt
     1681 ggaatacctg cgcgaacgat tgcctcaatt gtatttgcca tttcctgtcc gccctcgagc
     1741 tttatcgaat cgcaagctcc ttctttatat agaataccag catttgtgat cgcttgttct
     1801 ttggagacat tgtaggacat gaaaggcata tctccgacca catgtgctcg tttcaccgct
     1861 ctagtgaccg gtttgatatg agcgagcatt tcatccatgg ttacaggtgt agttccgttg
     1921 tagccaagtt cgaccatgcc aagactatct ccaaccagaa ttatgtctat tcctgcttca
     1981 tccacaagca atccttgagg atatgaataa gctgtaatca tcgatatctt cttgccttct
     2041 ctcttcatcg agacaagatc ggggatagag agcttttgta cttcatctgg cattattagg
     2101 cctcctttaa ttaagatata taattcatca tatatcgaaa tagccaataa tacaatggta
     2161 aaacgcgctt agctgctatt tattggtctt atttctttca aatcaatact tgacttggct
     2221 accagctatg ttaagataat taagtcgttg ctaaagatgt cgtcgtgcat aatattagat
     2281 attcttagca ttgttcaatt gtttaaagaa atctttataa gaaaggcggg catattgatt
     2341 aggtaagaag catgaacttg ttcgattcga tagcatttga gttatcatta tacttttgat
     2401 cgaaatttcc aaaattcact taaagagggg aggtctctta aaatgaacac tgttgagaag
     2461 tataagaatt atgttaatac ttctttcgtg aaggcgatcg agcctgttat cgttacgaat
     2521 gcatctggca gcaaaataac cagcgaagat ggtaaagtat atacggatct atattcaggt
     2581 atatcggtag tgaacgccgg gcattgcaat ccagaagttg cagaagcggc caaagcacag
     2641 attgataaat taattcattg tggcacctat gtttattatg cacctacagt ggcagacttt
     2701 gccgagaagc ttgcagaagt cgcacctgga agattgcaga agactttttt cggcaatggg
     2761 ggagcggaag ccaacgaatg cggaatgcgc atggcaaagc agtatacaaa aaaacaggaa
     2821 ttcattgctt tgcagggatc ctttcatgga agaagcttgg ccacattgtc cataacaggg
     2881 aataaagcta ggaagaaggg aggcggccca tatattccag gcgttgcctt caacccagct
     2941 ccatattgtt atcgatgtgg tttcgatatg tcgtatcctt cttgtgattt gtattgtgca
     3001 aaatatcttg aacgcacaat acaatatgat actagtgata atgttgcggc atttgttgca
     3061 gagacaatca tgggagaagg tggcataatt gtgccgccaa aagagttttt taaggaaatc
     3121 aagaaaatct tagatcagta tggtatccta cttttccttg atgaagtgca agctggtttt
     3181 ggtcgttccg gcaagctttt tgctattgag cactatgatg tcgaaccaga tatcatgact
     3241 atggcaaaag ggattgcagc aggctttcct ctatctgcgt gtatagcaag gccagaaatt
     3301 gcggattcct tccgaccagg agatcatcta tcgacttttg gtggcaatcc tgtttcttgt
     3361 gcggcagcca ttgcaaatct gaattatatg ctgagagaaa agcttcctga aaaagccatg
     3421 gaaaaaggga agattttcat agaggaattg aaggaaatac agaaaaaaac accgctcatc
     3481 ggggatgttc gtggctccgg gctaatgatt ggcgtagagt tggttcgtga taaaagaaaa
     3541 acacctgcag ccgaagaagc agccaagatc aagactctta tgagggagaa aggattcctt
     3601 atcgggttgg ggggtacatt tgcaaatgtc ttgaggttgc agcctccttt ggtcatttct
     3661 tcggaagagc tttcttctgc ggcaaacgct ctttctgaat gctttagaga attagtatga
     3721 aaatttctgt tattggatcc ggggccatgg gctctctctt tgggggtaag cttgctatag
     3781 cgggagagaa tgtggttctc tatgatgtct atagagacca tatagataca gtgaataaag
     3841 agggtctttc tatagaggat gccgagactg gaaagattac agttgtttac ccacaagcat
     3901 cctctgatcc tgaatccgta aagaattccg atgttctgct tattttcgta aaatcgacaa
     3961 acacagagtc ggtcgccaat caattcaaat cctttgctgc tccgc
//
LOCUS       DAOOWY010000143         3975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_22845_length_3975_cov_10.838265, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000143 DAOOWY010000000
VERSION     DAOOWY010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22845_length_3975_cov_10.838265"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1508
                     /gene="tig"
                     /locus_tag="PK105_08270"
     CDS             153..1508
                     /gene="tig"
                     /locus_tag="PK105_08270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOU61750.1"
                     /translation="MEIAEKRIEELELSRIRMTITVRAQDLRSEYDAMINEYAKQARI
                     DGFRKGHVPISVLERKFGPSLKTDAKARVVEKALETALKDVSRPPLAYEMPELENDPS
                     FELDSDFTFSVSYDAFPVFELPSLDGIEIEIPAVAITQEDIDRELEDIRQRNAIVVEK
                     DGPSEIGDIVIASFVELAEDGSEISGTSRQDFSFELGKNLNIYKFDDEIVGLKAGDEK
                     TIVKTFPDDFEYPEYAGKTVTIKVSVSKVKHQDLPDLDDELAQDVSEKYKTLDDLKEA
                     VRAHLTENLQTRLRKAKEHAIMDELLKKTKISIPRSMLATEIAMRWESLKQQMGVDSE
                     EHLEMLLEMSGKTREQIYNEWGPLAEKALATNILLDKLIEAGSYTSTDEEVDAEIAKE
                     ASHSTMSPAEIKTEYEKHGTIKYIKEDIAIRKFFDDILSSTTVKESGQMSYLDFMKKN
                     D"
     gene            1575..2186
                     /gene="clpP"
                     /locus_tag="PK105_08275"
     CDS             1575..2186
                     /gene="clpP"
                     /locus_tag="PK105_08275"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOU61751.1"
                     /translation="MSEYMSNLVPIVIEQTGIGERSYDIYSRLLKDRIVFIDGEISDV
                     IADLVIAQLLFLESQDPSRDISIYINSPGGLVTAGLAIYDTIQYIKPDVQTICLGQAS
                     SMAALLLACGTKGKRYALPSSRILIHQPWGGVSGQASDISLQAREIIRLKKLTIDYFA
                     LHSGQPIEKVAQDMERDFYMSSQEAVEYGIVDTIMEPRKHGKE"
     gene            2173..3426
                     /gene="clpX"
                     /locus_tag="PK105_08280"
     CDS             2173..3426
                     /gene="clpX"
                     /locus_tag="PK105_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOU61752.1"
                     /translation="MARNKQFDLEQETFCSFCGKSSSYVRKMITGPGVNICDECVRIC
                     SQILDEEEQRVTASFLTEVPKPKEIKEFLDMYVIGQEYAKRVLSVAVYNHYKRLVHKV
                     SAQSPDVEIEKSNVLLIGPTGSGKTLLARTLARKLKVPFAMADATTLTEAGYVGEDVE
                     NILLKLIQNAGGNIQAAERGIVYIDEIDKISRKGENPSITRDVSGEGVQQALLKIIEG
                     TISNVPPQGGRKHPNQEYLRIDTTNILFICGGAFVGLDKIIESRISSHPMGFGADVKA
                     RHEKMLSQLYSELQPDDLIKFGLIPEFVGRLAVKVALDELTKEDLKRIIIEPKNALLK
                     QYKASMQLDNVELEFDDAAIDAIAEKAIVQKTGARGLRAIIEKIMVDIMYSLPSDNNI
                     KKVILTRDAVEGKGSCIFEYRQKTA"
     regulatory      3430..3540
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3527..>3975
                     /locus_tag="PK105_08285"
     CDS             3527..>3975
                     /locus_tag="PK105_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU61753.1"
                     /translation="MMRNKQFIAIFVVIAMASIAVGTVSAQTSTQSNRLNNELIIWTY
                     DSFISEWGPAPLIAQAFQKEAGIKLRFVSKGDGGALLSALFDPINKPSADIIIGLDDK
                     QLEKALKSGLFAPLNLKNLSEIQKDLLLDKTNRLVPFDFSQFALIWDS"
BASE COUNT         1223 a          840 c          922 g          990 t
ORIGIN      
        1 tggatgtcgc gggtccaact cccgtttccc gcttcgacaa cagggcgatt cggaaactgg
       61 ccgaatcgcc ctgttttttt gtgttatagc acgctatata gcatatagcg cgcattttcc
      121 aatttatata gagtaagaac tgagaggatt atatggaaat cgccgaaaaa agaattgaag
      181 agcttgaact ctccagaatt cgcatgacaa ttaccgtacg agcacaggac ctgcgatctg
      241 aatatgatgc aatgataaat gaatatgcaa aacaagctag aatcgatggg tttagaaagg
      301 gacatgttcc tatttctgtg ttggagcgga agtttggccc atcgctcaag accgatgcta
      361 aggcgcgagt cgtagaaaaa gcgctagaga ccgccctcaa ggatgtctct cgaccaccac
      421 tcgcctatga aatgcctgag ctggaaaacg atccaagctt cgagctggat tctgatttta
      481 cttttagcgt tagctatgat gcttttccag tatttgagct tccttcgctt gatggaatag
      541 agatcgaaat accagccgta gccatcacac aggaagatat agatcgcgaa ttagaagaca
      601 taaggcaaag aaatgccatt gtcgtagaga aggatgggcc ttcggaaatc ggcgatattg
      661 tcatcgctag ttttgtcgaa cttgcagaag atggaagcga gatttctggt acttcacgtc
      721 aggacttctc tttcgagctc ggcaagaatt tgaacatcta taaattcgat gatgagattg
      781 tcggcctcaa ggctggagat gaaaaaacca tcgtaaaaac ctttcccgat gattttgagt
      841 atccggagta tgcaggaaaa actgtgacca taaaggtctc agttagcaaa gtgaaacacc
      901 aagaccttcc agatcttgat gatgaactag cccaggatgt ttccgaaaaa tacaagaccc
      961 tagatgacct aaaggaagca gtccgcgctc acctgacaga gaatctgcaa acaaggcttc
     1021 gcaaagcaaa agagcatgca attatggacg aactcctgaa aaaaacgaag atatccatcc
     1081 cccgttcaat gctcgctacc gagatcgcga tgcgatggga atccttaaag caacagatgg
     1141 gagtcgattc cgaagaacat ctggaaatgc ttctagaaat gtccggaaaa actcgtgaac
     1201 agatttacaa cgaatgggga ccgctggcag agaaggccct tgcgacaaac atccttctag
     1261 ataagctgat agaggctggg tcttatacat ctaccgatga agaagtcgat gctgaaatag
     1321 ctaaggaagc aagtcattcc acgatgagtc cagccgagat caaaacagag tatgaaaagc
     1381 atggcacgat aaagtatatc aaagaagata tcgccattag gaaattcttc gatgatatct
     1441 tgtcttcgac aacggtcaaa gaatcaggtc aaatgtccta tttggacttt atgaaaaaaa
     1501 atgattagat tttatttcgg agttcaatat tgaatctcaa ttatggcaaa tcaaaaggta
     1561 ccagagaaat ggcaatgtct gaatatatga gcaatctcgt tccgattgtt atcgagcaaa
     1621 ccggtattgg agagcgatcc tatgacatct attcgcgcct tctcaaagat cgtatcgtat
     1681 tcatcgatgg cgaaatcagt gatgtcattg cggatttggt gattgcacag cttttgtttc
     1741 tggaatctca ggatccctcc agggatattt caatctatat aaacagccct ggtggattgg
     1801 taaccgcagg acttgcaata tacgatacaa ttcaatatat aaaacccgat gtgcagacaa
     1861 tttgtctggg acaagcatcg agtatggctg ctcttttgct cgcctgtggg acaaagggta
     1921 agcgctatgc cctgccctct tcccgtattt tgatacatca accatggggc ggagtctctg
     1981 gtcaagcaag cgatatcagc cttcaagcgc gtgagatcat tcgattgaaa aagcttacaa
     2041 tcgattactt tgcgctccat tctggccagc caattgaaaa agttgcccaa gatatggagc
     2101 gagatttcta tatgagctct caagaagctg tcgaatacgg gatcgtcgat acgatcatgg
     2161 agccgcgaaa gcatggcaag gaataaacaa ttcgatctgg agcaggagac attctgttct
     2221 ttctgtggga aaagttcatc ctatgtccgc aagatgatta cagggcccgg cgtcaatata
     2281 tgcgatgagt gtgttcgtat ttgcagtcaa atcctcgatg aggaagaaca gagagtcacc
     2341 gctagttttc ttacagaagt acccaaacct aaagagatca aagaattcct tgatatgtat
     2401 gtgattggac aggaatatgc caagcgagtc ctttccgtgg ctgtttataa ccattacaaa
     2461 cgactagtac ataaagtatc agcccagtct ccagatgtcg agatcgaaaa gtctaatgta
     2521 ttgctcatag ggccgaccgg atcaggtaag acgctacttg cgcgcacttt agcccgaaaa
     2581 ctcaaagtac ctttcgctat ggctgatgca acgactctta cggaagctgg atatgtcggc
     2641 gaagatgtcg agaatattct cctcaagctt attcagaatg caggtggaaa tattcaggca
     2701 gcagaacgcg gcatcgtgta catagacgag atagataaga tttcacgcaa aggcgagaat
     2761 ccttcgatta cgcgcgatgt atctggagaa ggagtccagc aagctcttct taaaatcatc
     2821 gaaggaacca tctctaacgt acctccgcaa ggaggacgaa aacatccaaa tcaggagtat
     2881 cttcgtatcg atacaaccaa tatcctcttc atctgtggcg gagcctttgt tgggctagac
     2941 aagataatcg aatcgagaat ttccagccac cccatggggt ttggtgcgga tgtaaaagca
     3001 agacatgaaa agatgctaag tcagctctat tcagagttgc aaccggatga tcttatcaaa
     3061 ttcggcctta tccctgagtt tgtcggacgc ctggcggtaa aagttgcact ggatgaactt
     3121 acaaaagaag acctcaagcg tatcatcatc gaaccgaaga atgccttact caagcaatac
     3181 aaagcctcta tgcagctcga taatgttgaa ctggaatttg atgatgctgc aatagatgct
     3241 attgctgaaa aggctattgt tcagaagaca ggagcgcgcg gcttacgcgc tattattgaa
     3301 aaaatcatgg tggatataat gtactcgctt ccaagtgata ataatataaa aaaggtaatc
     3361 ctgacgcgag atgcggtaga aggcaaaggc agctgcatct tcgaatatcg tcaaaaaaca
     3421 gcctaagcgc agaaatcaat ggggtggttt gcttttctcc gttggagaaa gcagcctgag
     3481 aaacacccat cgaacctgat ccggataatg ccggcggagg gataagatga tgcgtaacaa
     3541 acaattcatc gcaattttcg tagtgatagc aatggcttca attgctgtcg ggacagtatc
     3601 agcccaaaca agtactcagt caaatcggct gaataatgag ttgataatct ggacctatga
     3661 ttcattcatt tcagagtggg gaccggctcc tcttattgct caagctttcc agaaagaagc
     3721 tggaataaag cttcgctttg tgtccaaggg agacggtgga gcgctactgt ctgcgctatt
     3781 cgaccccata aacaagccta gcgccgatat aataatagga ctcgatgaca aacaactcga
     3841 gaaagctcta aaaagtggcc tctttgctcc gctcaatcta aaaaacctct cggaaatcca
     3901 gaaggattta ttgctggata aaacaaatag gcttgtacct ttcgatttta gccaatttgc
     3961 gctaatctgg gactc
//
LOCUS       DAOOWY010000144         3973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_22855_length_3973_cov_61.655436, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000144 DAOOWY010000000
VERSION     DAOOWY010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22855_length_3973_cov_61.655436"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PK105_08290"
     CDS             complement(<1..535)
                     /locus_tag="PK105_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61754.1"
                     /translation="MKKPSSGKAGDKVVLRTIPKELGQEPSRMTDSPQQKATSQMQEQ
                     MHVQMPARSAQDYRPILSAKDLSIGYRYALPIVSGICFDFPVPFSARGNATVIYKKRK
                     NILGFVGPNGAGKTTFLKTCIGLLSPLGGELRLLDTDTMSSTFKETRKKLAYIPQNKP
                     EGNSGQLRISVREAVSFG"
     gene            complement(501..1313)
                     /locus_tag="PK105_08295"
     CDS             complement(501..1313)
                     /locus_tag="PK105_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61755.1"
                     /translation="MAIGYSHHKAQSNLSPQAFVRLVRLRRLLSLALICLIAIPLAAQ
                     EQLVIASTSWVQAYAVAAGAKNIITIAPFELQHPPEYEMKPSDLIAVQNASLIVYSSY
                     EKFAKKLAETAQNKNIKIIAVYTDNVPSNIIAESKKIAEALGTTEEHAKWAQSFTSFT
                     NDMRAKIVASLPDKRVAVQAYVKTFAVWLGLDVVGTFGPGEPSPAVVLDLIKKKPAMI
                     LDNYHNPGGKALAESLGVPNVLLINFPGKDGTRTIEDVFLYNEKAILGQSWR"
     gene            complement(1598..2050)
                     /locus_tag="PK105_08300"
     CDS             complement(1598..2050)
                     /locus_tag="PK105_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HOU61756.1"
                     /translation="MENRPIIAVIDGLGGGIGCQLCARIRQAFGQRVEILALGTNSTA
                     TEKMIKAGADRGASGENVMQVSIGFAQFILGPIGIVLPDSLMGEITPTIAQSIMRASG
                     KKILLPVSQPHFALAGVSPVPLSKLIEEAVNLLATELDESLANKPEKQ"
     gene            2154..>3973
                     /locus_tag="PK105_08305"
     CDS             2154..>3973
                     /locus_tag="PK105_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009044 - xylan 1,4-beta-xylosidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 52 protein"
                     /protein_id="HOU61757.1"
                     /translation="MESNRKNIDYYSLHGIWGAYASFVLGRMERGAGVVVGDVRPPER
                     GLFVGYRVGAGETCLLPFSSGRKYGLGVTAYLSGESFQNIDEHYKKARRFNPEEIERQ
                     IYFSGEEWRAKSMRFRIYSFFGEVPDPAIVSLASARSAYRPSILLELSFDNSDGKEAM
                     TGLFGMQGIRRPLSDSTNGALLGMASNDCFGFGIRPAADVEEVMDWSVINATFNCSRP
                     LCRLASEGGLRFKIPAHSKAKYIIALGVYRDGIITSGRRAFAYYSNLFKDLEDVIENA
                     LDQAEESLCKAQRLDDMLESSGLSEDRCFLIAQAAHSYVANTELLRTEAGEPVFIVSE
                     GEYQMMNTLDLVIDQAFWEARFSPWTLRNELESLEEYSSYEDSYGLAFAHDQGVDNCF
                     APRGRSVYEMPGLTGCFSFMSYEETLNWTISACIYSNLAEDWKWALQKRKVLSSCLES
                     LIARDENGDGIMDRDSDRCEGGAEITTYDSLDISLGQARNNLYLAVKAWSAFVCLEAL
                     FKRIDGKETDESRTAAKAAGLAASSIAANMVEPDGFIPAIFESENRSHIIPAIEGLVY
                     PKFCGASKAVAIDGPYRELVCALKRHLENVLVPGLCIDSYS"
BASE COUNT          967 a          899 c          975 g         1132 t
ORIGIN      
        1 tgccaaaaga aaccgcctct cgcaccgaaa tacgcagctg ccccgaattt ccttctggct
       61 tgttttgtgg gatataagcc aattttttac gcgtttcttt gaatgtcgac gacatcgtat
      121 cggtgtcaag aagccgtagt tctccaccta acggagacaa aagtccaata caggtcttga
      181 gaaaggttgt tttgcctgcc ccatttggac ctacaaatcc aaggatattc tttcgtttct
      241 tatagataac tgtcgcattg cctctggctg aaaaaggaac tggaaaatcg aagcatatac
      301 cgctgacaat tggtaaggcg tatcgatatc ctatggagag atcctttgcc gataatattg
      361 gccgataatc ttgtgcgctc cgtgccggca tctgtacatg catctgctcc tgcatctgtg
      421 aagtagcctt ttgttggggg ctatctgtca ttcttgacgg ctcttgacct aattcttttg
      481 ggattgttcg aagcactacc ttatctccag ctttgcccga ggatggcttt ttcattgtag
      541 agaaatacat cctctatcgt gcgggtgcca tcctttcccg ggaaatttat aaggagcaca
      601 tttgggacac cgaggctttc ggctaaagct ttaccgcctg gattgtgata gttatctaag
      661 atcatcgcag gctttttctt tattagatcg agaacaaccg ctggcgatgg ctctccagga
      721 ccaaatgtgc caactacatc gagccctagc cacaccgcaa aggttttgac ataagcttgc
      781 acggcaaccc ttttatctgg cagcgaagcg acaatcttgg ctcgcatgtc gtttgtaaac
      841 gatgtgaatg attgagccca ttttgcatgc tcttcggtgg tacccaaggc ctctgcgatt
      901 tttttcgact ctgcgataat gttcgagggg acattgtcgg tatagacggc tattattttt
      961 atgttcttat tttgggctgt ctctgcgagt ttctttgcga acttttcata actagaatag
     1021 acaatcaatg aggcattttg caccgcaatt aggtcggatg gtttcatttc atattcgggg
     1081 ggatgctgaa gctcgaacgg agcgattgta atgatattct ttgcgcctgc ggccactgca
     1141 tatgcctgca cccatgaggt ggaggcaatg acaagttgtt cctgagcagc aagtggaata
     1201 gcaataaggc agataagtgc gaggcttaga agcctgcgaa gacgcacgag ccgaacaaat
     1261 gcttgcggcg atagattgct ctgtgcctta tgatgagaat acccgatagc catagaggat
     1321 cctttggaaa tgaagataat ttaggcaggc tttgagctca ccttaggaag tgcttcgagc
     1381 acttcgattt tcaatgaatg gccagaaggc gacgcttctg gccgcattca tttaggcgct
     1441 tttagcaatt cagccccaag gtataaattc gcgcagcatt aaattcggcc cgcattcatt
     1501 tagggcaaaa ttcggtatac tctggccttc gggccagtag gttcttgcca gattattcta
     1561 cttacctttc tatcatatct gcaagtacaa atcttttcta ttgcttttct ggcttatttg
     1621 caaggctctc atctagctcg gtggcaagaa ggttcaccgc ttcctcgatc aatttcgaaa
     1681 gaggcacagg agatactccg gcgagcgcga aatgaggctg gctcacgggg agcaatattt
     1741 tcttgccgct cgcgcgcatt atggattgcg ctatagttgg cgtaatctcg cccatcaagg
     1801 aatcgggcaa tacaatgcca atagggccaa gaatgaattg tgcaaagcct atggaaactt
     1861 gcatcacgtt ttctccactt gctcctcgat cggcgccagc tttgatcatt ttttcagttg
     1921 ctgtggagtt ggtgccaaga gccaagattt ctacgcgttg tccgaaggcc tgacggattc
     1981 ttgcacaaag ctggcacccg attcctcctc caagcccatc gattacagca ataatcggtc
     2041 tgttctccat gagagaaaga tagaatcaat cgattgattt tgcaacaata ataaggctat
     2101 cctcttgcat ataggttttc gcaatgacta gcgacctaaa tggaagttta tcgatggagt
     2161 ctaatagaaa aaatatcgat tactactcct tgcatggtat atggggagcc tatgcttcct
     2221 tcgttcttgg gcgtatggag cgcggcgcag gagtagtagt cggagatgtc cgccctcccg
     2281 agagaggatt gtttgttggg tatcgcgttg gagcggggga gacttgtctt cttccttttt
     2341 cttcggggag aaaatatggc ctaggcgtta cagcatatct ctctggcgaa tcattccaaa
     2401 acatcgatga acattacaag aaggccaggc gctttaatcc ggaggagatc gaacggcaaa
     2461 tctacttttc aggcgaagag tggcgcgcga aatctatgag gtttcgaata tactcttttt
     2521 ttggggaagt tcctgatccg gccattgttt ctctagcctc tgctcgttcg gcttataggc
     2581 catctatcct tcttgagctt tcattcgaca atagcgatgg caaggaagcg atgacagggc
     2641 tctttggaat gcaaggcatt cgccgccctt tgtcggattc caccaatggg gctcttcttg
     2701 gtatggcatc taatgattgc tttggttttg ggataaggcc agccgccgat gtggaagagg
     2761 tgatggattg gtccgttatc aatgccacat tcaactgtag ccgtccgctg tgccgtctag
     2821 cctccgaagg aggccttcgc ttcaagatac cagcgcattc caaggctaaa tatattattg
     2881 cgctaggggt ttatcgcgat ggtatcataa cttcgggtcg ccgcgccttt gcttactatt
     2941 caaatctctt taaggatctc gaagatgtca tagagaatgc gctcgaccaa gccgaagaat
     3001 ctctttgtaa agcccaaagg ctcgacgata tgctggaatc ctccggcctc tcggaggata
     3061 gatgttttct cattgcacaa gctgcccata gttatgtagc aaatactgag cttctaagaa
     3121 ctgaagccgg tgagcctgtt tttattgtca gcgaaggcga ataccagatg atgaacactc
     3181 tcgatcttgt catagaccaa gctttctggg aagctcgttt ttcgccttgg actcttcgaa
     3241 atgagcttga gtctctagaa gagtattcct cttatgaaga ttcctatggc cttgcgtttg
     3301 cgcatgacca aggggtagat aattgttttg ctccaagagg cagatccgtc tatgagatgc
     3361 ccggacttac gggttgtttc agctttatga gctacgagga aaccctaaac tggacgatct
     3421 cggcttgtat ctactcgaat cttgctgaag attggaagtg ggccctgcaa aagcgtaagg
     3481 ttcttagttc ttgcttagaa tctctaatcg cccgcgatga aaacggcgat ggaatcatgg
     3541 atagagactc agatcgatgc gagggaggag cggagattac aacctatgat tctctcgata
     3601 tcagtttggg ccaagcacga aacaatctct atctggctgt aaaggcctgg tcggcattcg
     3661 tttgccttga agcccttttc aagagaatcg atggaaaaga aacagatgag tcgcggactg
     3721 ccgcaaaagc agctggcttg gcagcgtcta gcatcgcagc aaacatggta gagcccgatg
     3781 gctttattcc tgctattttc gaaagcgaaa atcgatctca tatcattcct gctatagaag
     3841 gattggtcta tccaaagttc tgcggtgctt ctaaggccgt agctatagat ggcccatata
     3901 gagaattggt gtgtgcctta aagcgccatc tggagaatgt tcttgttccg ggtttatgca
     3961 tagacagcta ttc
//
LOCUS       DAOOWY010000145         3867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_23635_length_3867_cov_17.731112, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000145 DAOOWY010000000
VERSION     DAOOWY010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23635_length_3867_cov_17.731112"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..3444
                     /locus_tag="PK105_08310"
     CDS             175..3444
                     /locus_tag="PK105_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61758.1"
                     /translation="MLNLGMLVLKAIYEFFCNNSLENKLFPLKPACNARASHNCVHNM
                     HSHKICTHKSHNKGFYNLRVIIALFFFCALVQTQAQLIPSIKPVLHFRTDCFDIYAPQ
                     SLEADAKRLAGFADNTYGELCEFLGLGLETKRIPVLLSDIEYSLNGFSTLFPSNRIVL
                     LLSAADPRDQLATFEDELYSVFLHELVHYVTLNERSLAWRVLAWLGGDWIAPEIYMMP
                     QAIVEGTAVWVESRFKENNSGRLNDPAAMEFVRLERARRENRSLWDVSGLEDFYGAGS
                     LPYLYGGLFVDYLSERFGKSIVARLWQSGGRGNIYQGFVGGILVKGILERETGIEFDQ
                     LWQDFLAWVDEESDLEMQGEQGEIDGIGENSAAELPEGQELFSGYIGALGAGGGKVYF
                     VDLERHGLYQLSLVDLEENSERQPDLEKEKEEKSEVAEKQDKQEMLERLNKPKRPERL
                     FAADQMLRNIYCDVNFGGLELDWNRINPQNQLIPARYLYDLSEHKLSYEKDLPQAKIG
                     EALFETHSSSKEEIFLHDSWQDPLTSIEYGLVRLGTAVLPARRLANMEIEVADIPDSA
                     IRWLSQGFRDNSESSDSICFALSLVPDNGLSRLAILEQDNGLWRFAIARKSPLGGVHM
                     PVFADASHIVYKASSENGQSSLLLLDISSFRNEIETPGRFLDSEFLNWISPTEWIESY
                     SLKMKNQSAHLDSTEEAQSQPELSKVEQPRQETNKLAQPEQEFSLEPCSATQKATQKR
                     SMLFPESFETSRIPYAYGTLLGIGFIAMDLTERLSWSAFVGWDWLIARPASSIDLSLS
                     AGAWSFEICAWDQGIFAFPVARISALEGALDWEHTLVPTFRSISAGIHARYGAVQNKY
                     EEQGLFHQSPDYFAWVAGTGVEFDSLSSSYRPPYDPSGISLKGGIEYEGANSLKFGGV
                     SLSGSIYLGESTIAGRIGASIYGAWAPTGGVAFSPATRYLEYAGDHELSVADLPYPAY
                     KEYQACSELSAWYLYGEIKARLFSLELNWIMSNLFAPSLALRRISMISGLRGAGLEID
                     NAPYLLSSAFLQAKFDCAFLSGVVAATHMKFTLEAAWAFQADKAGGEPFCFSVGLSGQ
                     F"
     gene            3635..>3867
                     /locus_tag="PK105_08315"
     CDS             3635..>3867
                     /locus_tag="PK105_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOU61759.1"
                     /translation="METSFSPPLDPCTYDLRIGTVGYEYSEWEGVLYPNGIEKETYLR
                     LYCEHFSTLELDFTFYTMPKAAQMRDILARALGS"
BASE COUNT         1027 a          804 c          949 g         1087 t
ORIGIN      
        1 gtggtgagtg tgtcgtcgat gtccatgaga atataattaa tctttttcgc ttctttgtcg
       61 gtgagagagc ttatgggtat aagactaata ctactaactc tatccatgtc agggccttgg
      121 taaaataaag actagggtat cataaacagg ggcttgctct gcgtcaagga agatgtgcta
      181 aacttgggca tgcttgttct caaggctatc tatgagtttt tctgcaataa cagcttagaa
      241 aataagctct ttcctcttaa gcctgcttgc aatgcgcgtg caagccataa ttgtgtccac
      301 aatatgcata gccataagat atgcacccat aagtctcata ataagggctt ttacaatctg
      361 cgcgtcataa ttgccttatt ctttttttgt gctcttgtac aaacccaggc acagcttatt
      421 ccttctatta aaccagtgct tcattttaga accgattgct tcgacatata tgcaccgcaa
      481 agcctagaag ccgatgcaaa gaggctagcc ggctttgccg ataataccta tggagaacta
      541 tgcgaatttc taggactagg gctagagaca aagcgcattc cggtgctttt aagcgatatc
      601 gagtattcct tgaatggatt ttctaccctt ttcccttcca atagaatcgt gctgctttta
      661 tctgctgcag atccgaggga tcagcttgca acctttgaag atgagctata ttcagtgttt
      721 ttgcatgagc ttgttcatta tgtcacccta aatgagcgga gtcttgcttg gagagtactg
      781 gcatggctcg gaggtgactg gattgctcca gagatataca tgatgccgca ggccatagtc
      841 gaagggaccg ccgtgtgggt ggagagtagg ttcaaagaga ataattccgg taggctgaat
      901 gatccggcgg ccatggaatt tgtcaggctg gagagggcgc gcagggaaaa tcgatcgctt
      961 tgggatgttt ccggcttgga ggatttttat ggagcaggtt cactgccata tctatatggt
     1021 ggtctatttg ttgattattt gagcgaacgt tttgggaaat ccattgttgc aaggctatgg
     1081 caaagcgggg gtcgcggaaa tatctatcaa ggattcgtcg gaggaattct tgtaaaagga
     1141 attctggaac gcgagacggg aatagaattc gaccagctat ggcaggattt tctagcatgg
     1201 gttgacgaag aaagtgattt ggagatgcaa ggagagcagg gcgaaatcga tggaatcgga
     1261 gagaacagtg ccgcagagct acccgagggg caggagcttt tttcaggata tattggggct
     1321 cttggtgctg gagggggaaa agtctatttt gtggacctag agcgccatgg cctttatcag
     1381 ctctcattag tggatttgga agagaactct gaaaggcagc ctgatcttga aaaggagaag
     1441 gaagagaagt ctgaggttgc agaaaagcaa gacaaacaag agatgctaga aaggctgaat
     1501 aagccaaaaa ggccagagag actttttgca gctgatcaaa tgttacgtaa catttattgt
     1561 gacgtaaatt tcggaggact ggaactagac tggaatcgaa tcaatccgca aaaccaactt
     1621 attccagcac gctatctata cgatctctct gagcacaagc tcagctacga aaaagacctt
     1681 ccacaggcta agatcggcga agcattattc gaaacacatt cttcttcaaa agaagagata
     1741 tttctccatg attcatggca ggaccctctt acgtccatcg aatatggcct tgtaaggctg
     1801 ggcactgcgg ttcttcctgc ccgaagactc gccaacatgg agatcgaggt agctgatatt
     1861 cctgattcag ccatacgatg gctttcccaa ggttttcgag ataattccga gtcatccgat
     1921 tctatttgct ttgccttgag cctcgtgcca gataatggat tatcgcggct tgctatttta
     1981 gagcaagata atgggctttg gcgatttgca atagcaagaa aaagtcctct gggtggtgtt
     2041 catatgccag tcttcgcaga tgcttctcac attgtctata aagcatcctc tgaaaatggg
     2101 caatcgagcc ttcttcttct cgatatttca agttttagga atgaaatcga aacgcctggt
     2161 cgatttttgg actctgagtt ccttaattgg atttctccga cagaatggat agagagctat
     2221 tctctgaaaa tgaagaacca gtcagctcat ctagatagca ctgaagaagc acaatctcaa
     2281 ccagagctta gtaaagtcga gcagcctcga caagagacca acaaattggc tcagcccgaa
     2341 caagaattct ctctggaacc ttgcagcgcc acacaaaagg ccacacaaaa gcgatctatg
     2401 ctttttcccg aaagcttcga aacaagtcga atcccctatg cttatggcac attgctcggt
     2461 ataggattca tagcgatgga tcttaccgaa cggctatcct ggtcagcttt tgttggatgg
     2521 gattggctaa tagcaagacc agcttcaagc atcgacttaa gtttgagtgc cggagcttgg
     2581 agcttcgaga tctgtgcgtg ggatcaaggg atctttgctt ttcctgttgc gcgcatatct
     2641 gcccttgagg gggcgctgga ctgggaacat acccttgtgc ccacatttag aagcatttct
     2701 gcagggatcc atgctcgata cggagctgtg caaaataaat atgaagagca aggcttgttt
     2761 catcaatcac cggactattt cgcatgggta gcggggaccg gagtcgagtt cgatagtctt
     2821 tcttcttctt atagaccacc atacgatcct tccggtattt cgctcaaggg cggaatcgaa
     2881 tatgaaggcg ctaattcact gaaatttggt ggtgtttctt tatcaggatc gatctatctg
     2941 ggagaatcga caattgcagg acgaatcggt gcttccatct atggagcctg ggctcccacc
     3001 ggaggagtag ctttttcgcc agcaactcga tatctggaat atgctggaga ccatgagttg
     3061 tcggtagcag atcttccata tcctgcatat aaagaatatc aggcttgctc agaactctct
     3121 gcctggtatc tatatggaga gataaaggcc aggctctttt ctttagagct aaactggatt
     3181 atgagcaatc tctttgctcc ttctttagca ttgagaagaa tatcgatgat ttcaggattg
     3241 cgcggtgcgg gccttgagat cgacaatgca ccttaccttt tgagttcagc ttttctccaa
     3301 gccaagttcg attgtgcttt tctctcaggc gttgtggccg caactcacat gaaattcact
     3361 ttggaggcgg cttgggcttt tcaagctgat aaggcaggtg gagaaccctt ctgcttttct
     3421 gtagggctga gtgggcaatt ttagtttgct tcaaactatc tcgatagaga gtatagaaag
     3481 aatcttgcgg atcatcgcag agaatcagtg tgcttttcat gaaatgtgtc ccattgcaca
     3541 agaaggcttc cttatgccat taccaattct tataaacaag gttcgtagca gtatgttcct
     3601 tgataactac acatctgtat agtataattc aaatatggag acaagctttt ccccaccact
     3661 cgatccttgt acctatgatc tcagaattgg gacagtgggg tatgaatatt ctgaatggga
     3721 gggggtgctg tatcctaacg gaatagaaaa agaaacatat ttgcgcttat attgtgagca
     3781 cttttcgacg cttgagctgg attttacatt ttataccatg cccaaggccg cccaaatgcg
     3841 agatattctc gctcgagctc tagggtc
//
LOCUS       DAOOWY010000146         3843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_23812_length_3843_cov_60.123284, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000146 DAOOWY010000000
VERSION     DAOOWY010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23812_length_3843_cov_60.123284"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PK105_08320"
     CDS             <1..602
                     /locus_tag="PK105_08320"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU61760.1"
                     /translation="VVPCPEDGELAPLGEYGRNKIEAERLCREACAAGLPVVMLRPPT
                     ITGSGLNEPFILSLLSAIQKGKPVTLLGKGTNRFQFIHVDDVVDACLLAAEHPDAAGE
                     AFNIGGKDVPSIAELVHLVASAVGSPSKVRLIPTTPAKIAIALLRPFGAAPLEPEHVA
                     IAICDYVFDWSKAQRMLGWEPRWSVADALIDTYRSIYGG"
     gene            606..3485
                     /locus_tag="PK105_08325"
     CDS             606..3485
                     /locus_tag="PK105_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61761.1"
                     /translation="MSLVDNASKALVSGRIPSPLVRGAAHVVVAILSAKMRIDDAKGS
                     RRATKAALKLPAESTSEHPSGAVPEKLYFVDGYHGGVIFWDWLILPGGGLWRTYNWPL
                     CVRPVIKRALHDSRFKVVLDFDAYTYEWMAKHDSHATSLLRSALEAGALEIVNGTYGQ
                     PFGCAESGESFIRQLELGNRAIEAALGSSPEVFYSQEPTYFAQLPQILSLMGYSGAVF
                     RTQWAAFGTDPAHDEDLVTWVAPDGSEIPTVPRYSFQNYKRQLEHHPGLAAGSLGIGD
                     KPDWAPESFPAFLEAASACGIAHPFISDLKDVNLPEAPLPRATEIVAMKNIVFTRPKD
                     YLRIANPPQEKVSHGPHDTPCTLPWGLEADDVPRSVAAAESALRVAESLDAVLHFFKP
                     EYASAESSLTDAWKSLCIAQQHDLYVCGPWLSLAHRQPMWRVAVDFAEHARKEAERIT
                     LEAMASISTDSSSSSAFASGSICPSSGTSGGIIVFNAASYERRDYVEVKMPKAALPQD
                     KPQGKSELCAYSNDELYSCQLISEDESEICLGFVPKLQGLSLKHFSLMQVPTQHSETE
                     DSISPPFTILPTAKDGLTLCSSSGEKLIEGLCFTCVRDGKPYNSLLSTKTAKWVANGP
                     VLYSLELEGNVADLPFILTLKMYSGVKRIDASVRFDFGDGPTYLGPQMDDYPQGTPYS
                     IHDEAKLCVSFNVPAECICCKSPFWISEVSGERITGAPWIGLESDERKHPGEHKREHL
                     AFINRGNRGYHWNKEEHRLRQVLAWAPREWIYASDDSFTRGKSAFTALSGTRTFEFAI
                     APYADRIEAERHSMNYATPLIAIWQDSSEAEASKSAPTSNTTPSDHIQSSSALAVDSE
                     KIFVTAFLVQNGNIIARLWNASGNEVTSALQSHPNVPLFEKVPTSRSSSAPGSKPAPA
                     SASASRSGQGSKAGSEPNAECLSWRNIDGHKLTLRPWGIHTLKIG"
     gene            complement(3548..>3843)
                     /locus_tag="PK105_08330"
     CDS             complement(3548..>3843)
                     /locus_tag="PK105_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61762.1"
                     /translation="NRNGYDLAANRKYIRCCSHGSIWKEPNDWMMGLKQKERTLVAFW
                     DEETGADMSSTVANIMLEANATSSSSGYLSLSYEFDNAQAAFDEREASESSVF"
BASE COUNT          967 a          982 c          928 g          966 t
ORIGIN      
        1 aagtggtccc ttgccccgag gatggcgagc ttgcacctct aggagaatat ggcaggaaca
       61 aaatcgaagc tgaacgactt tgccgtgaag cttgcgcagc gggtcttcct gtcgtcatgt
      121 tgaggcctcc gactattaca gggtctggcc tcaatgagcc ctttatcttg agccttttat
      181 ccgctatcca gaagggaaaa cccgtgaccc ttcttggtaa aggaacaaat agatttcaat
      241 tcattcatgt cgatgatgtt gtagatgcat gcttgctcgc tgccgagcat ccagatgcag
      301 ctggagaagc attcaatatt ggtggaaagg atgtgccaag catagctgaa ttggttcacc
      361 tagttgcctc tgcggtaggt tcgccatcga aagtgaggct cattcccaca acgccagcaa
      421 agatagccat agccctcttg agacctttcg gtgctgctcc attggagccc gagcatgtcg
      481 ctatagcgat ctgcgattat gttttcgatt ggtctaaagc acagaggatg cttggctggg
      541 aacctcgctg gagtgtagct gatgcgctta tcgacaccta tcggtctatt tatggagggt
      601 aattcatgag tctagtggat aatgcctcta aggctttagt ctcaggccgc attccttcgc
      661 ctctggttcg cggcgcagct catgtagttg tcgcgatcct tagcgctaaa atgcgtatag
      721 atgacgcgaa gggttcaagg cgtgcgacaa aagccgcctt aaaattgcct gctgagtcca
      781 cttcggaaca tccatctgga gctgtgcccg agaaactata ttttgtggat ggatatcatg
      841 gtggcgtgat tttctgggac tggctcattt tgccaggagg aggtctctgg cgaacgtata
      901 actggcctct ctgtgtgcga cctgtcataa agcgtgcgct ccatgattcc agattcaagg
      961 ttgtgctcga tttcgatgct tacacctacg aatggatggc aaaacacgat tcacacgcga
     1021 ctagccttct tcgcagcgcc ctggaggccg gtgcgctcga aatcgtaaat gggacatatg
     1081 gtcaaccatt tggctgtgca gagagcggag agtctttcat caggcagctc gaactaggca
     1141 atagagccat cgaggccgcg ctcggttcat caccagaggt attctattcg caagaaccaa
     1201 cgtattttgc tcagcttccg cagatcctgt ccctgatggg ctattcagga gctgtcttca
     1261 gaacacaatg ggccgctttt ggcacagacc cggcccacga tgaggatctt gttacatggg
     1321 tggctcctga tggcagcgag attcctacgg tacctcgata ttcctttcag aattacaagc
     1381 ggcagctcga gcaccaccct ggtcttgccg caggttccct tgggatagga gataaacccg
     1441 attgggcgcc tgaaagcttc ccggcttttc tggaggctgc ttcggcgtgt ggaattgcac
     1501 acccctttat ttcggatcta aaggacgtaa atcttcccga ggcgccattg cctcgtgcta
     1561 cagagatagt agcaatgaag aatatcgtat tcacgagacc taaagactat cttcgaattg
     1621 ctaatccgcc acaggaaaaa gtctcccatg gcccacatga tactccttgc acactaccct
     1681 ggggcctaga ggccgacgat gttccgagat cagtggccgc agcggaatca gctcttcgcg
     1741 tcgcagaaag tctcgatgcc gtgctccact ttttcaagcc tgaatacgcg agcgcggagt
     1801 catctcttac ggatgcatgg aagtccctat gtattgctca acagcacgac ctctatgtct
     1861 gcggtccctg gctttcgctt gcccataggc agcctatgtg gcgtgttgca gtagactttg
     1921 cggagcacgc tcgcaaagag gctgagcgaa taactttaga agctatggca agcatttcaa
     1981 ctgattcttc tagcagtagc gcttttgctt ctggcagtat ctgcccttct agcggcactt
     2041 ctggtggcat aattgtcttc aatgcggctt catacgaacg ccgcgattat gtcgaggtca
     2101 aaatgccaaa ggcagcacta cctcaagaca aaccacaagg gaaaagcgaa ctttgtgctt
     2161 attccaacga tgaactatat tcatgccagc tcatctcaga agacgaatct gagatatgtc
     2221 ttggttttgt accaaagctg cagggcctct ccctcaagca tttttctctt atgcaagtac
     2281 ctacacagca tagtgaaaca gaagacagca taagtcctcc attcaccata ttgcctacag
     2341 ctaaagatgg acttactttg tgctcttctt ctggagaaaa actaatcgaa ggcctctgct
     2401 tcacctgtgt gcgcgatggc aaaccttaca attcgctctt atctacgaaa acggcaaaat
     2461 gggtcgcgaa cgggcctgtt ctctattcac tcgaactgga aggcaatgtg gctgatctac
     2521 ccttcatcct aactctaaaa atgtattcgg gtgtaaagag aatagatgca agcgttcgct
     2581 tcgatttcgg agatggcccg acatatctgg ggcctcaaat ggatgactac ccacagggca
     2641 cgccctattc gatacatgac gaagcaaaac tctgtgtttc cttcaatgtc cccgctgagt
     2701 gcatctgctg caaatctcca ttctggatat cagaggtctc tggtgagcgg ataacaggtg
     2761 cgccatggat tggcctcgaa tctgatgaga gaaagcatcc cggagaacac aagagagagc
     2821 atcttgcttt catcaataga ggcaataggg gctatcattg gaacaaagaa gagcataggc
     2881 ttcgccaggt cctcgcctgg gctcccagag agtggatcta tgcaagcgac gactccttta
     2941 cacgagggaa atcagccttc acagccttgt cgggcacacg caccttcgag tttgctatcg
     3001 ctccctatgc ggaccggata gaagcagaaa gacactcaat gaactatgct acacccctga
     3061 tagcgatttg gcaggactca agcgaagcag aagcatctaa atctgctccg acaagcaaca
     3121 caacgccctc cgatcatatt cagtcctcca gcgccctcgc ggtagattca gagaaaatat
     3181 ttgtcacagc cttcttggta caaaatggga atatcatcgc aaggctctgg aatgcttccg
     3241 ggaatgaagt gacgagcgcg ctccaaagcc atcctaacgt ccctcttttc gaaaaagtcc
     3301 caacctcaag atcaagctca gcgccaggat caaaaccagc tcctgcatca gcttctgcat
     3361 caagatcagg gcaaggttca aaagcaggct cggaaccaaa cgcagaatgc ttatcgtggc
     3421 gaaatatcga tggccataag cttactctgc gcccatgggg aattcataca cttaagatag
     3481 ggtgaggaag tttattgagc atcaattaaa aaggggcaca atcttgcacg accttagatt
     3541 catcaatcta gaaaactgaa gattccgagg cttcgcgctc atcgaatgca gcttgagcat
     3601 tatcaaactc ataggaaagg gaaaggtatc cactgcttga acttgttgcg tttgcttcga
     3661 gcataatatt cgcaactgta gatgacatat ctgctccagt ctcctcatcc cagaatgcta
     3721 caagtgtcct ctctttttgt ttgagaccca tcatccaatc gtttggttct ttccaaatgc
     3781 ttccatggct gcagcacctg atatactttc gatttgctgc aaggtcatac ccatttcgat
     3841 tcc
//
LOCUS       DAOOWY010000147         3832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_23889_length_3832_cov_9.768599, whole genome shotgun sequence.
ACCESSION   DAOOWY010000147 DAOOWY010000000
VERSION     DAOOWY010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23889_length_3832_cov_9.768599"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PK105_08335"
     CDS             <1..529
                     /locus_tag="PK105_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU61763.1"
                     /translation="VGEQFEKGGVPKDYYEIPEGLPVIRKPGKDITIATYGATLYRAM
                     EAAKILEEKYGLSIEVMDLRFLTPFDYDPLVESVKKTGRLVLASDAVERGSYLHTIAS
                     NVEQFAFDWLDAPIAVVGSRNWITPAAELEQIFFPQPEWIVDAVNERILPLKGHESST
                     IQTTGDLARRYRIGV"
     gene            574..>789
                     /locus_tag="PK105_08340"
     CDS             574..>789
                     /locus_tag="PK105_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU61764.1"
                     /translation="MNEPGELEKIINDAKNEPVLRLEVLAQLAETMGRPAARSGESNN
                     HIHTCYSFSPYTPSGAALAARRAGLDVA"
     assembly_gap    790..799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <800..1943
                     /locus_tag="PK105_08345"
     CDS             <800..1943
                     /locus_tag="PK105_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU61765.1"
                     /translation="DHDSYAAASEMRAACALLGIGVVTGFELRVSLSEAARSFPEKTS
                     MMLTTHKLNNPDSIGIVYMTIQAIPAPAIKEVEAFLAPIRRARYQRSAHMAELANKIL
                     FPLGLPEIDFQKDMVANSKYAEGGTITERHLLAAVSRSILSQVEPGLQLIKWLEEKLN
                     IVLSNSQKRNLSESQNPYLVYDLLGALKSGFLDRIFIQPTEECVDVRDAISFAKKIGA
                     IPAYAYLGDVDESPTGDKKAQKFEDEILDELIPVLKELGFPAITYMPPRNTKEQLLRL
                     QRLCRRYDLMEISGVDINQPRQSFRCPELGLPEFRHLDDATWAMVAHEALSGLDRRFG
                     LFSAENSLAKMPLAERIRRYASIGRAIDIAEPGSLKKQAELSLLEK"
     gene            1946..3301
                     /locus_tag="PK105_08350"
     CDS             1946..3301
                     /locus_tag="PK105_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU61766.1"
                     /translation="MNITINSENLAAIVRGCILSMCDYSCMVQSMGYAPESDRTDQSV
                     SRSSQLKEISFTINKLTAGAWMDDFEAARLIKEAIEPFASDIRESLFQSAPQGRLQES
                     PLGTARDAPQRTPEKAPEEVSKKASKGVVSLSIVTSKGRSIAHYTLIPLAPIDPHIRQ
                     EVNQPGDPSLLPSSARSEVVRGKISLVTGGAQGFGAEIARGLAFSGAAVWIADINLSG
                     ATCFAEKLRAETGTDAIFAVEIDVSKEESVERAFDTVARACGGLDLVVSNAGILRAGS
                     VLEQSADEFRMVNDVNYIGFFNVAQHASRLLRRQWLGAPEWYTDIIQINSKSGLEGSN
                     KNAAYAGSKFGGIGLVQSFALELVDYNIKVNAICPGNFFGGPLWSDPLNGLFVQYLHS
                     GKVPGATTVEDVQGYYESKIPMRRGCYGPDVLKAIYYLVEQVYETGQALPVTGGQVML
                     N"
     gene            3383..>3832
                     /locus_tag="PK105_08355"
     CDS             3383..>3832
                     /locus_tag="PK105_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOU61767.1"
                     /translation="MTEAIAVLDIGMTNKKVVLYSNELALIGEKKKVFPPLMRDGIET
                     HDLKAMEEWFLASLGEFAASFSIGAIAVTTHGATFVCTDEKGNPVTPCIYYTYEPSLE
                     FHERFYERAGKPEDLQERTGTPNFSALINPAKGLFFLKENYPLEFAKA"
BASE COUNT         1001 a          942 c          963 g          916 t
ORIGIN      
        1 tgtgggcgag caattcgaaa aaggcggtgt gcctaaagac tactatgaga ttccagaagg
       61 cctacccgtt attcgcaaac ccggtaaaga tataaccata gcaacctatg gtgcaacctt
      121 atacagagct atggaagccg ccaaaatcct cgaggaaaag tacgggcttt caatagaggt
      181 aatggatctt cgcttcctca cgccattcga ttatgatccg ctcgtagaga gcgtcaaaaa
      241 gacgggccgg ctcgtcctgg cttcggatgc ggtagaacgc ggttcatacc tgcacaccat
      301 tgcttctaat gtagaacagt tcgccttcga ttggctcgat gcgcctatcg ccgtagtagg
      361 gtcgcgcaat tggattacac cagccgcaga gctcgaacaa atcttctttc cgcagcccga
      421 atggattgta gatgcggtaa acgaacgaat cctcccactc aagggtcatg agtcttcgac
      481 tatccagacc actggtgatc ttgctcgccg atatcgaatt ggagtatgag atggtgagga
      541 gtcgtgctgg atgcaagaaa aaggaaataa gcgatgaatg agccaggaga actggaaaag
      601 atcataaatg atgccaaaaa tgagcctgta ttgcgcctcg aggttttggc ccagcttgcg
      661 gaaacaatgg gcagacccgc tgcccgatcc ggcgaatcga acaatcatat tcatacctgc
      721 tacagcttca gcccttatac accttcgggc gcggccttgg cggcgcgcag ggctggcctc
      781 gatgttgcgn nnnnnnnnnc gaccacgact cctacgcggc cgcgagcgaa atgcgcgccg
      841 cctgtgcctt gctaggcatt ggggtcgtaa ccggttttga gctcagggtc tcgctatctg
      901 aagctgcacg gagcttcccc gaaaagacat ccatgatgct cacaacccac aagctcaaca
      961 accctgattc tataggcatc gtctatatga ccatccaggc catacccgcc cccgcaatca
     1021 aagaagtcga agcctttctt gctccgatta ggagggcaag atatcagcgc agcgcgcaca
     1081 tggcagagct ggcaaataaa atcctctttc ccttgggact accggagata gatttccaaa
     1141 aagatatggt tgctaactcg aagtacgcag agggtggcac aataaccgaa cgccatctcc
     1201 tggctgccgt atctcgatcg attctgtcac aggtggaacc tggtctgcag ctcattaaat
     1261 ggctggaaga gaagctgaat attgtccttt ccaattctca gaagcgaaat ctctcggaat
     1321 ctcaaaaccc ctatctcgta tacgatcttt tgggagcctt gaaatccggg ttcttggata
     1381 ggatttttat tcagcctacc gaagaatgtg tcgatgtaag agatgccatc agctttgcaa
     1441 agaaaattgg cgcaatcccc gcgtatgcat atcttggcga tgtcgatgaa tctccgacag
     1501 gcgacaaaaa agctcaaaag ttcgaagacg aaatattgga tgaactaatt cctgtgctaa
     1561 aagaacttgg ctttcctgcg ataacttaca tgccacctcg caacaccaaa gaacagctct
     1621 tgcggcttca gaggctttgt agaagatatg atctcatgga gatatcgggg gtcgacatca
     1681 atcagccacg ccaatcattt cgctgtcctg agctcggact gcctgaattt cgacatttgg
     1741 atgatgctac ctgggcgatg gtagcgcatg aggcgctcag tgggctcgac cggcgctttg
     1801 gtcttttctc tgcagagaat tcccttgcca agatgccgct tgccgaacga atccgccgtt
     1861 atgcatccat tggccgggct atcgacattg cagagcctgg ttcgcttaaa aaacaagcag
     1921 aactctcgct tttggagaaa tagctatgaa tatcacaatc aattctgaaa accttgcagc
     1981 tattgtgcga ggttgtattc tttcaatgtg cgactattcc tgcatggttc aaagcatggg
     2041 ctacgcgccc gagtcagatc gcactgatca gtcagtttct cgcagtagcc agctcaaaga
     2101 gatatctttc actataaata agctcacagc tggcgcatgg atggatgatt tcgaagctgc
     2161 aaggctcata aaagaggcaa tcgagccatt tgcaagcgat attcgagaaa gcctattcca
     2221 aagtgcgccc caaggaaggc ttcaagagtc gccactgggg acagcccgag atgcgcccca
     2281 aagaacgccc gaaaaagcgc ccgaggaagt gtccaaaaaa gcatccaaag gcgtggtgag
     2341 cctttcaatt gtaacttcaa agggccgatc cattgctcac tataccctta ttccgctcgc
     2401 tccgatcgac ccgcacattc gccaggaggt caatcaacca ggagatccaa gccttctccc
     2461 cagcagtgcg cgatcagaag ttgtaagggg caaaatttcg ctggtaactg gcggagcgca
     2521 gggctttgga gctgagatag cccgaggact tgcattctct ggcgcagcgg tctggattgc
     2581 agatatcaat ctatctggcg caacctgctt tgctgaaaaa ctgcgggcag aaactggcac
     2641 agatgcgatt ttcgcggttg aaatcgatgt cagcaaagaa gaatctgtcg aacgagcgtt
     2701 cgatacagtc gcccgcgcgt gcggagggct cgaccttgtt gtgtctaatg caggcatcct
     2761 gagagcagga tctgtgctcg agcagtctgc agacgagttt cgcatggtca acgatgtgaa
     2821 ctatatcggt tttttcaatg tggcacagca tgcatcgcgc cttttacgtc ggcagtggct
     2881 tggagcaccc gaatggtata ccgacatcat tcaaatcaat tccaaatctg gcctagaagg
     2941 atcgaataaa aatgctgcct atgcaggatc gaaattcggc ggtattgggc tcgtacagag
     3001 ctttgcgctt gagcttgtcg attataatat caaggtgaat gcaatctgcc caggaaactt
     3061 tttcggagga ccgctctggt cagacccgct gaatgggctt tttgttcaat atctccattc
     3121 cggcaaagtg cctggcgcta ccacagtcga agatgtacaa ggatattatg aatccaagat
     3181 tccaatgaga agaggctgct acgggcccga tgtgctcaaa gcgatctact accttgttga
     3241 gcaggtatac gagacaggac aggctttgcc tgtaacgggt ggacaggtta tgctcaattg
     3301 atgttttctt gttgcatggt cgcgctctct agtatatttg gatgtttcct ggtccattcg
     3361 tttttaagag aagggaggct ctatgaccga agcgattgcg gtgctcgata tcggaatgac
     3421 aaataaaaaa gtcgtgctct attccaacga acttgcattg atcggagaaa agaaaaaagt
     3481 cttccctcca ctgatgcgag atggcattga aacacatgat ctcaaggcca tggaggaatg
     3541 gtttcttgca tcacttggag aattcgccgc gagcttttcc attggggcaa ttgcggtgac
     3601 cactcatggc gcaacctttg tctgtaccga tgaaaaggga aatcctgtta cgccctgtat
     3661 ctattatacc tatgagccga gccttgaatt tcacgagcgt ttttatgagc gtgctggcaa
     3721 gcctgaggac ctgcaggaaa gaacagggac ccccaatttc tcagcgctca ttaatcccgc
     3781 aaaaggactc tttttcttga aggagaatta tccgctagaa tttgcaaaag ct
//
LOCUS       DAOOWY010000148         3827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_23931_length_3827_cov_8.411983, whole genome shotgun sequence.
ACCESSION   DAOOWY010000148 DAOOWY010000000
VERSION     DAOOWY010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23931_length_3827_cov_8.411983"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1828
                     /locus_tag="PK105_08360"
     CDS             350..1828
                     /locus_tag="PK105_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU61768.1"
                     /translation="MIDLAIDESQRKKNIERCKKRGILLPTFAQMRDPTKISATIKAE
                     LSRIGLWDVHPRNLFRVNWHNEPKEFGGGFGSVNYIEIPRAISGTKARIIGLAGKWFP
                     TGAHKVGAAYACLAPELVTGRFDPTTKKAVWPSTGNYCRGGAYISRLLACPSVAILPE
                     GMSRERFDWLKTMAEEVIATPGSESNVKEIFDKCLELQRTRHDVVIFNQFDQLPNHLW
                     HYAITGPAIEEVFATLNGSAVPASGACAHVCGVVLASGSAGTLGSGSYIKEKFPGAKL
                     AVAEALQCPTILENGFGAHRIEGIGDKHIPWIHNLRDTDAAVGVDDELTIRLIRLFNE
                     PAGQKLLLEVGTDPSIVEKLDLLGISGVGNLIAAIKFAKYYELGEDDIVFTMFTDSME
                     LYQSRLAELTEERGLYDQRQADRDYECLQGLSVDYVFEMTHVDKRRVHNLKYFTWIEQ
                     LGKELPELRAQWSDYRNYWSGLHEQVCALDMLIEDFNAEVLK"
     gene            1825..3186
                     /locus_tag="PK105_08365"
     CDS             1825..3186
                     /locus_tag="PK105_08365"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOU61769.1"
                     /translation="MILLKDCLRVVQLKAEDLCGVDVLVEGNRITQIAKNIAVPCGSR
                     VIDASHLVVAPGLVNTHHHFYQTLSRNLPAVQTAKLFDWLVYLYEIWKGIDEEAVYVS
                     SLLAMAELIKTGCTISTDHHYIYPQGFETDIPTLQFKAASDIGMRFLATRGSMSRSKK
                     DGGLPPDSVVQDEDTILEHCEQTIQKFHDSASDSMRRVALAPCSPFSVSEELMRESAI
                     LARKYGVRLHTHLAETSDEEDYCVQMYGRRPLRLMEDCGFLGADVWYAHGIFFNDEEL
                     ETLAKSKTGIAHCPSSNMRLGSGICRVREMLDIGVPVGLAVDGSASNDSSDMLGEIRQ
                     ALLLQRIRYGATGLTSKEAWQMATKSGAEILGWEQAGSIEVGSLADIACWNVDKLAYA
                     GSLSDPSAALLFCGYDHAAEMVIVNGKIVVDNGRIVCIDEEKLKEDANMVALRLYKKA
                     GIA"
     gene            3250..>3827
                     /locus_tag="PK105_08370"
     CDS             3250..>3827
                     /locus_tag="PK105_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOU61770.1"
                     /translation="MDRNRIKKLIDDLRSKKIAMCKKDFLLTWEKSLPEIQATFLVAD
                     ILRAMRESNISPKVWDSGIAVSNFRDNSTRTRFSFASACDLLGLYVQDLDEGKSQVAH
                     GETVRETATMISFLTEAIGIRDDMYLGEGDRYQREVGTALDDGVKAGVLPSRPTIVNL
                     QSDIDHPTQAMADFLHLVHYFGGLDKLKGKKIT"
BASE COUNT         1007 a          939 c          954 g          927 t
ORIGIN      
        1 ctgcaaaagg acatagagcc caagcaataa aaccaagaga gaagtaataa acatgggaat
       61 ggaatatgga ttcaagaaat agtttgcact attcaagagt tggctcagca tctaaccccc
      121 ttgctcaatc gagctatgcc caatccaaag agatgcccta ctaacttata ttctgcttca
      181 ttcaagagcc attttcaaga cttttgactt taaatacatt tatggcttgc cttgctcgat
      241 aaatatggcg caaacagata aactctcgca gaaagcagct tcgcatgtta taatatattt
      301 tctatcactt gctgataatc ccatataata tcattgtaag gagttcggca tgatcgatct
      361 agccatcgac gaatcccaaa gaaaaaagaa catcgaacgc tgcaagaaaa gaggaattct
      421 gcttccaact tttgcccaga tgcgagatcc cacaaagatt tcggccacaa taaaggccga
      481 actctccagg attggccttt gggatgtaca tccgcgaaat ctctttagag tcaactggca
      541 caacgagccc aaggaatttg gaggtggttt cggatcagtc aactatatag agattcctcg
      601 agcaatctct ggcaccaagg ctcgaatcat cggacttgca ggcaaatggt tccctacggg
      661 tgcacataag gtcggcgcag cctatgcctg cctcgcgccc gagctcgtca cgggacgctt
      721 cgatcccacg accaaaaaag ccgtgtggcc gagcaccggc aactactgcc ggggcggcgc
      781 ctacatctcc cgcctcctcg cttgcccgag cgtcgcaatt ctccctgagg ggatgagccg
      841 cgagcgcttc gattggctta agaccatggc agaagaagtc attgctacac ctggcagcga
      901 atctaatgta aaagagatct tcgacaaatg ccttgagctg caaaggactc gccatgatgt
      961 tgtcatcttc aatcaattcg atcagctacc caaccatctt tggcattatg ctatcaccgg
     1021 cccagccata gaagaagtat tcgctacgct caacggctcg gctgtccctg caagcggagc
     1081 atgcgcccat gtttgcggtg tagtgctggc ttccggctca gcgggaacac tcgggtcggg
     1141 ctcttatatt aaagaaaaat tccccggcgc aaaactcgcg gtagcggagg cgctgcagtg
     1201 ccccacaatt ctagagaatg gttttggggc acatcgcata gaaggaattg gtgacaagca
     1261 tattccctgg atccacaatc ttcgtgacac cgacgcagca gttggagtcg acgatgaact
     1321 tacaattcga ctcattaggc tcttcaacga accggctggc caaaaacttc tcttagaagt
     1381 gggaactgat ccttccatag tcgaaaagtt ggatctgctt ggcatatccg gagtgggaaa
     1441 tctcatcgct gccataaagt tcgcaaagta ctatgaactg ggcgaagatg atatcgtatt
     1501 cacaatgttc accgactcga tggagctcta tcagtcccgt cttgcggagc tgactgagga
     1561 gcggggttta tacgatcagc gccaggccga ccgcgactat gaatgcctgc agggcttatc
     1621 ggtcgattat gtctttgaga tgacgcatgt tgacaagcgg cgtgtgcaca acctcaagta
     1681 tttcacctgg atcgaacagc tcggaaagga acttcccgag cttcgagctc agtggagcga
     1741 ttatcgcaac tattggtccg gtctgcatga gcaagtatgc gcgctcgata tgctcatcga
     1801 agacttcaat gcggaggttc tgaagtgatc ctcttaaagg attgccttcg agtcgttcaa
     1861 ctaaaagctg aagatctatg cggcgtcgat gtactggtgg aaggaaatcg gataactcaa
     1921 attgcaaaaa acatcgcggt tccttgtggt tctcgtgtta tcgacgcttc gcatcttgtt
     1981 gtagcgccgg gcttagtcaa tacacaccat catttctatc agaccctttc gcgcaatctg
     2041 cccgccgtgc agactgccaa gctcttcgat tggcttgttt acctctatga aatatggaaa
     2101 ggtatagacg aagaggcggt ctatgtctcg agccttttgg ccatggcaga attgataaag
     2161 acgggatgca caatctcaac ggatcaccat tacatatatc cacagggttt tgaaacagat
     2221 attcctactc ttcaatttaa ggctgcatcc gatatcggca tgcgctttct cgccacccgc
     2281 ggttctatgt cccgatcgaa gaaggatggt gggcttcctc ctgattctgt agtacaggac
     2341 gaggatacaa tcctagaaca ctgcgaacag acgatccaaa aattccacga ctcagcttct
     2401 gattcgatgc ggcgtgttgc gcttgctcct tgtagccctt tttcggtctc cgaggaactg
     2461 atgagagaat cggcgatcct ggccaggaaa tatggagtga ggttgcacac tcatttggca
     2521 gaaacctccg atgaagagga ttattgtgtc cagatgtatg gccgacgccc tcttaggctc
     2581 atggaagact gtggctttct tggagccgat gtttggtatg ctcatggtat cttttttaat
     2641 gatgaagagt tagagacgct tgccaaatcc aaaacaggta ttgctcattg cccctcgtcc
     2701 aatatgagac tgggctctgg catatgccgc gtaagagaga tgttggacat cggagtacct
     2761 gtggggcttg ctgtggatgg ctctgcttcc aatgatagtt cggacatgct cggcgagata
     2821 agacaggcgc tgctgctgca gcgcatacgc tatggtgcta ccggcctgac ttccaaagaa
     2881 gcctggcaga tggcaactaa aagcggtgct gagatcctgg gttgggagca agctggttcc
     2941 atcgaagtag gatccctggc agatatagct tgctggaatg tcgacaaact tgcatatgca
     3001 ggctcgctat cagacccctc ggctgcactc ctgttctgcg gctatgacca tgccgcagag
     3061 atggttattg tcaatggcaa gatagtggtc gacaatggcc gcatcgtctg catcgacgag
     3121 gagaagctaa aagaagatgc aaatatggtc gctcttcgat tatacaaaaa ggcaggaata
     3181 gcataagaat tatgggagaa gaatgcctcg atcttggaag gagattctct cttaaaggag
     3241 atttttggaa tggatagaaa tagaatcaag aagctcatag atgatcttcg cagcaagaaa
     3301 atcgcaatgt gtaaaaagga tttcttgttg acgtgggaga agtccctccc agaaatccag
     3361 gccaccttcc tcgtggccga tatccttcgc gcaatgagag aaagcaatat ttcgcctaaa
     3421 gtctgggact caggcatcgc ggtgtcgaac ttccgcgaca attcgactcg cactcgcttt
     3481 tcatttgcat ctgcatgcga tctgctcggt ctctatgtgc aggacctcga tgaaggcaaa
     3541 agccaggtcg cccatggcga aacagtgcgc gaaacagcca ccatgatcag cttcctcact
     3601 gaagcaattg gcatacgcga cgacatgtac cttggagaag gcgaccgcta tcagcgcgaa
     3661 gttggtactg cgctcgacga tggcgtcaag gcaggtgtgc tgccttccag gcctacaatc
     3721 gtcaatctac agagcgatat cgaccatccg acacaagcta tggccgattt tttgcacctt
     3781 gtgcattact ttggaggcct cgacaagctc aaaggcaaaa agatcac
//
LOCUS       DAOOWY010000149         3668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_25290_length_3668_cov_9.666759, whole genome shotgun sequence.
ACCESSION   DAOOWY010000149 DAOOWY010000000
VERSION     DAOOWY010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25290_length_3668_cov_9.666759"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1945
                     /locus_tag="PK105_08375"
     CDS             <1..1945
                     /locus_tag="PK105_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61771.1"
                     /translation="RRIASLSTSCGSAVSSRQTGACMNQKLYHLRWIREVWRERQALV
                     WLLLFLTLVSSAVAVAFPLLTKQLFDLLEHAIDTKMSEDVAMAQVKRIALYFAAIGVA
                     GLIAGIFPGVRGALNMVFDYIVRKRYFGEVVRKDIRFFSEFRSGDVITRLTSDISDFP
                     KISWFLCSGIFRAVESASKVTFCIIAMFSLDWRLALASLASLPVMLFVFSRTQAAIYD
                     RVNKNEQAISEINNQLEMSFSGVRVIKAFASEEKYTSFFEDALKKRFNTEMAVIKLET
                     VMHLIYQYIDYVAQIALVFVGGLMVTKRSISIGTFYAFYNYLNMLIYPVLDIPQLFVF
                     GKRAFVNIDRLEEIRQYETGSQKQCATSTQKKYDPSIQNQNKVSAQRQEKSTPKKDIA
                     SDKAFRSLCFENVTLRYEQRRKAALSDISLEILPGEKFLIIGPVGSGKSTLIKTALGL
                     LEPQKGRVMINNKPVRSLDPTEIRGLIGYVPQDPLLFSGSIRENILFGCREGQNLTDE
                     ELFRIIEVSRLTEEIEYFSDGLETKLGQRGTSVSGGQKQRIAIARALAGYPALLLLDD
                     MTASLDTNNEEALWHSLAAMPEFAQAAVIAVSHRLSSIQYVDRVLFLNGGRIAGFGKH
                     TELMEQNGLYREFVAEHVRRPTG"
     gene            2119..3135
                     /locus_tag="PK105_08380"
     CDS             2119..3135
                     /locus_tag="PK105_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61772.1"
                     /translation="MERTIRLLTFILMILVVGSVDAQQRIVLAGRGANMLADAIYLFP
                     DAGKRLVAYSSGDQGLGLFMSEVDPAFKEKVCFDKSAGVEVIASFKPDLVLLKSISRS
                     QYEAALNALGIRQVYLMLETPEQYYEDIATLGKVLGNQVHAAEVISWYADHEKKIMSR
                     TTKLSASQKPKVLLLQLAASGESVWKVPPDSWMQTILAERAGGIPVWKGANLGSGWAT
                     VSVEQIAAWNPDVVCIINYRANSSEAAEAFKKDKRLSSLKAVREGKVYGFPQDFYSWD
                     QPDTRWILGLTWLAKMLHPALFTDISVIGTTEDFFNFMYGFDEAAFHTNIAPKIQGDV
                     GEQF"
     gene            3137..>3668
                     /locus_tag="PK105_08385"
     CDS             3137..>3668
                     /locus_tag="PK105_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOU61773.1"
                     /translation="MKHECPVSAMIDVSSSSDSHLPSNRTYRAISQRSYLWFLLVILL
                     IAIAALSLFIGRYPKPGIMNPALLGTDSIARQIVLNSRLPRILGAILLGLVLGGAGAS
                     LQSIFSNPLIDAGFLGVSQGAAFGAALALVLGLRAYSVIAALAFGMAVLALALSIWLS
                     ERFRYGGQILRLVLAGL"
BASE COUNT          943 a          790 c          911 g         1024 t
ORIGIN      
        1 acgaagaatt gccagcctat cgacttcttg tggatcagca gtttccagca ggcaaacggg
       61 tgcttgtatg aaccagaaat tatatcatct tcgctggata cgtgaggtct ggagagaacg
      121 ccaagctctt gtgtggctct tgctattctt gacacttgta tcgagtgcgg ttgctgtggc
      181 ttttcctctg cttacaaagc aactctttga tttacttgaa catgcaatag atacaaaaat
      241 gagcgaagat gttgcaatgg ctcaagtgaa acgcatagcg ctctattttg cagcaatagg
      301 cgtagcgggg cttattgcgg gcatattccc tggtgtacgc ggagcgctca atatggtttt
      361 cgactacatt gtgcgcaaaa ggtatttcgg cgaagtagtg cgaaaggaca ttcgattttt
      421 cagcgaattc cgatcaggtg atgtcattac gcgcttgaca tctgacattt cagattttcc
      481 caagatctcc tggtttttat gttccggcat ttttcgagcc gtagagtctg caagcaaggt
      541 cacattttgc atcattgcaa tgttcagtct cgattggaga ctcgctctcg catctcttgc
      601 ttccctgcct gtaatgcttt ttgtgtttag ccggacacag gcggcgatct atgatcgtgt
      661 gaataaaaat gagcaagcca tatctgagat aaacaaccaa cttgaaatga gtttttcggg
      721 agtacgtgta ataaaggcct ttgcaagtga agaaaaatac acaagtttct tcgaggatgc
      781 tcttaaaaag cgattcaata cggagatggc tgtcataaag ctggagacag ttatgcacct
      841 catctatcag tatatcgact atgtggcaca gattgctctt gtgttcgtgg gcggattgat
      901 ggtgacaaag cgaagtatct cgataggaac tttttacgct ttctataatt atctcaatat
      961 gctcatttat cctgtgctgg atatcccaca gctctttgtg tttggtaagc gagcatttgt
     1021 caatatagat aggcttgaag agattagaca atatgagact ggatctcaaa agcaatgtgc
     1081 aacatcgact cagaaaaaat atgacccgtc gattcagaat caaaataaag tatctgctca
     1141 gagacaggaa aaatctacac caaaaaaaga tatcgcaagc gataaggctt ttcgctccct
     1201 ttgtttcgaa aatgtcacat tgcgatatga gcaacggcgc aaagcagctc tatcggatat
     1261 cagcctggag atattgccgg gcgaaaaatt ccttataata ggaccggttg gatcaggaaa
     1321 atccaccctt attaagaccg cactaggttt actcgaacct cagaagggac gagtaatgat
     1381 aaataataag ccagtgcgca gcctggatcc gacagagatt cgcggcctta taggatatgt
     1441 gccacaagac cctctcttgt ttagcggctc gatacgagag aatatcctgt ttggttgtcg
     1501 agaagggcag aaccttactg acgaagagct ctttcgtatc atcgaagtat ctcgtctcac
     1561 agaagaaatc gaatattttt cagatggatt agaaaccaag ctcggacagc gcggcacaag
     1621 cgtttcagga gggcagaaac agcgtatagc aatagcccga gcgctcgcag gctatccagc
     1681 tctgctctta ctcgacgata tgaccgcctc cctcgatacc aataacgaag aagccttatg
     1741 gcattcgcta gccgcaatgc cagaattcgc acaggctgcg gttatagcag tatcccatag
     1801 gttatcaagc attcaatatg tcgatcgagt gctctttttg aatggtgggc gcattgctgg
     1861 ttttggcaaa cacacagagc ttatggaaca aaacgggctc tatcgtgaat ttgtggcgga
     1921 gcatgttaga agaccaactg gctaaatcag cttagcaagc ccacaatctc gattttagag
     1981 caagaattat cgtccttatt ctatggttag atggtgtgcg acaattctat gcagaagtag
     2041 ccaggcagca tatcttcgag cccaataatc ctcttatttc ttgactggtc ttgctttggc
     2101 atctacaata atggaattat ggaacgcact attcgactct taacttttat tttaatgatt
     2161 ttggtggttg gtagcgtcga tgctcaacag cgcattgtct tagcagggcg cggagcaaat
     2221 atgcttgcgg atgctatcta tctttttcct gatgcaggga agagacttgt agcttattcc
     2281 agcggtgatc aaggtttggg gctcttcatg tcggaggtag atccagcatt taaggaaaag
     2341 gtttgctttg acaagagtgc gggggtagaa gtgatcgcgt ccttcaaacc tgatcttgtg
     2401 ctcctcaaat cgatatcgag aagtcagtat gaggcagcac tgaatgcgct gggtatccgc
     2461 caggtctatt tgatgctaga gacgcctgag cagtactatg aggatatcgc aacattgggc
     2521 aaggttcttg gaaaccaggt acatgccgca gaagtaatta gctggtatgc tgaccatgag
     2581 aagaaaatta tgagccgcac aaccaagctt tctgcgtccc agaaaccaaa ggtccttttg
     2641 ttgcagcttg ctgcttctgg agaatcggtc tggaaggttc cgcctgattc atggatgcaa
     2701 acaatcctag cggagcgcgc aggtggcatt cctgtatgga aaggagcgaa ccttggctcc
     2761 ggatgggcga ctgtatctgt tgaacagata gcagcttgga atcctgatgt agtgtgtatc
     2821 atcaattatc gtgcaaattc atcagaagca gccgaagctt tcaaaaaaga taagcggctt
     2881 tcttcgctaa aagcggttag ggaagggaag gtatacggtt ttccgcagga tttttattcc
     2941 tgggatcagc cggatacgcg gtggattctg gggttgacat ggcttgcaaa gatgcttcat
     3001 ccggcgttat tcaccgatat ttcggttatt ggaactacag aagacttttt caactttatg
     3061 tatggattcg atgaagcagc tttccacact aatatcgcgc ctaagataca aggcgatgtc
     3121 ggggaacagt tctagcgtga agcatgagtg tcccgtatcg gcaatgatcg atgtttcttc
     3181 gagctcagac tctcatctgc cttctaatag aacttacagg gcgatttcgc aacgttctta
     3241 tctgtggttt cttctcgtaa ttttgctcat cgcgatagcc gcgctttcgc tttttatcgg
     3301 acgctatcca aaacccggca taatgaatcc tgccctgcta ggaacagatt caatcgcgcg
     3361 tcaaatcgtg ctaaattctc gcttgccacg catcctggga gcgatattgc ttggacttgt
     3421 gcttgggggt gcgggagctt cgcttcaatc tatctttagt aatcccctaa tcgatgcggg
     3481 ttttctgggt gtatcgcaag gggcagcttt cggagctgcg ctcgcgctcg tcctagggtt
     3541 gcgcgcgtat tcggtcatcg ctgcgctggc cttcggcatg gcagtgctag cgcttgctct
     3601 ttcgatatgg ctttccgagc gattcagata tggcgggcag attttgaggc ttgtgctcgc
     3661 tgggctag
//
LOCUS       DAOOWY010000150         3627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_25669_length_3627_cov_45.468645, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000150 DAOOWY010000000
VERSION     DAOOWY010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25669_length_3627_cov_45.468645"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..3625)
                     /locus_tag="PK105_08390"
     CDS             complement(53..3625)
                     /locus_tag="PK105_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU61774.1"
                     /translation="MIADLHIHSRYSRATSPKLTLPYIERWARIKGINLVGTGDCTHP
                     AWLRELKEQLEPTEGGFFKLKDSVRKEFDSSEALTAELPDSDVHFVLSGEISTIYNRD
                     GKTRKVHHVVILPDFKAAAAFQARLARIGNISSDGRPILGLDSRDLFALLLDADERSI
                     LIPAHIWTPWFSALGARSGFDSIEECYGDLSSRIGAIETGLSSNPPMNWAVSALDSFA
                     IISNSDAHSPENLGREATIIDMEESFAGLYEALTKRGSTAEEAQSKISGASAGMVSGT
                     EAATGTVTGKVAETPTGTVAGTIEFFPQEGKYHYDGHRACKVFLSPEESTKLDGRCPV
                     CGKPLTPGVMRRVTELADRPVDENAPCPANWEGTNRRPYWSLIPLPELLAELLGTGSG
                     SKKVAAVYRGIVEQAGSEFALLMDKSEEEIKAIQTVGLSGELLALSIGRMRAGKVSIK
                     PGYDGEYGIIHVFAPGERIEARAEQSLFVEEKAPSELFDEGIETRGQFEKRAKEQNKE
                     SPTTSPTTSVPQKDKKEKQNGPNLSNRGHSVKTAFQLNPAQEAAVNHYGGPALVVAGP
                     GTGKTAILALRIARLVEQGVDPSSILAITFTNKAAAELRKRVELTVGAEKAAQINTKT
                     FHSFCLSVLREHAAEASLPQDFVVLNEEERLAFLESACKSDQGSNKREKSSSSLEPSP
                     SNIPGLNLPSEPPTTTSRSRKIKRLASYIEERKRFLLLPGDERPRLGPSSPAGLAELA
                     TELGCPPSNGSNPELELAYARYRDQLKQTHALDFDDLVAGTVRLLAARPALLASYRER
                     FHAIFVDEYQDVNFAQYALIRLLVPDQNPVEHYARELYVIGDPNQAIYGFRGSDKRFI
                     ERFVVDYPGAAVYRLLKSFRCAPGIMAAAGRLVDVDLSGSGKAVALSRSEFPTEASEA
                     EGIAREIDRLIGGTGFFAIDSDTIDTKERPALSSLGECAILVRAATLAPPIEKALIDH
                     GIPYRLIGDKPWWEEEPARSVLIFLRASIRSDSLDTNILKNPVDAVRFAADFINSEKT
                     IKKSKATALTDHESSLERLLAQASLYSDFSSFLDDLALGSPQDGYEENSECVSLMTIH
                     SAKGLEFDYVFVPCLEDGLLPFTIFEDDDYHKERIEEERRLLYVAMTRARIGLYLSWA
                     RSRHLFGRKLSLPPSRFLSDIEELVPLIENKTPRKPKDIQLTLF"
BASE COUNT          817 a          875 c         1001 g          934 t
ORIGIN      
        1 aatgcctgca tcgcaagcct ctcaaggaga gagctctcca aaagagctct ctctaaaaaa
       61 gtgtgagttg aatatccttt ggtttgcggg gtgttttatt ttcgataagc ggcacaagtt
      121 cttcgatatc agaaagaaag cggctgggcg ggagactgag tttgcgtcca aataggtgcc
      181 gcgagcgggc ccaggagaga taaagcccaa tgcgggctct agtcattgcc acatatagaa
      241 gcctgcgttc ttcttcgatt cgttctttgt ggtaatcatc atcctcgaag attgtaaagg
      301 gaagtagtcc atcttcaagg cagggaacga agacatagtc gaactcgagt ccttttgcgg
      361 agtggatggt cattagagag acacactcgc tgttctcttc gtagccatct tgagggcttc
      421 caagggctag atcgtcgagg aaggatgaaa agtcgctata tagcgaggct tgggctagaa
      481 ggcgctccaa cgaagattcg tgatcagtga gggctgtcgc tttggatttc tttatggttt
      541 tctcgctgtt tatgaagtcc gctgcgaagc gaacggcatc tactggattt ttgagtatgt
      601 tggtatccag agaatccgat cggatcgagg ctcgcaagaa aatgagcacc gatctagcgg
      661 gttcttcttc ccaccagggt ttatccccga tcaatcgata gggtatgcca tggtcgatga
      721 gggctttttc gataggagga gcgagcgtag cagcacggac gaggattgcg cactctccga
      781 ggctagagag ggctgggcgc tcttttgtat cgatagtatc ggaatcgatg gcaaaaaagc
      841 cagtgcctcc aattaggcgg tctatctcgc gagctatgcc ttcggcttcc gatgcttctg
      901 ttggaaattc ggatctcgaa agcgcaacgg cttttccaga gccactgaga tcaacgtcta
      961 caagcctacc ggccgcggcc ataattcctg gtgcacagcg gaagcttttc agaagtctat
     1021 agacggcggc acctggatag tcgacgacaa agcgctctat aaatcgcttg tcagagcccc
     1081 gaaaaccata gatggcctgg tttgggtcgc caattacata gagctctctt gcatagtgct
     1141 ctacagggtt ttgatcgggc acaagaaggc gtatcagagc atattgagca aagttgacat
     1201 cctgatattc gtctacaaag atggcgtgga atctttcgcg atatgacgcg agtaaggctg
     1261 gccgagccgc tagaagtcgc accgtgccag caacaagatc gtcgaaatcg agggcgtgcg
     1321 tttgcttgag ctggtcgcga tagcgagcat atgcgagctc aagctcgggg ttggatccat
     1381 tagatggcgg gcagccgagt tcggtagcca attcggcgag gccagccgga gagcttgggc
     1441 caagccgagg tctctcgtcg cctggtagaa ggagaaagcg tttgcgctct tcgatatatg
     1501 aggcaaggcg cttgattttg cgggaacgcg aagttgtagt gggaggctcg gaaggcaaat
     1561 tgagccctgg aatattggaa ggcgatggct ctagcgagct gctagatttt tcgcgcttgt
     1621 tgctgccttg atcggattta caagccgatt ccagaaaagc taatctctct tcttcgttga
     1681 ggactacgaa gtcctgcgga agagaggcct cggctgcatg ttctctcaaa actgaaagac
     1741 aaaaagaatg aaatgttttt gtgttgatct gcgctgcctt ctcagctccg acagtcagtt
     1801 ctacgcgctt tctgagttcg gctgcagcct tgtttgtaaa agtgattgcg agaatcgacg
     1861 atgggtctac gccctgttct acaaggcgag caatacgaag agcaaggata gccgttttgc
     1921 ctgtgccggg gcccgctacg acaagggctg gtcctccata gtgattgacg gcagcttcct
     1981 gtgccgggtt gagctggaag gctgtcttga cggaatggcc acgattagaa agattgggac
     2041 cattttgttt ttcctttttg tctttttgag ggacggaggt tgtaggcgaa gtcgtaggac
     2101 tttctttgtt ttgttccttt gcgcgtttct cgaattgccc tctagtctct attccttcat
     2161 cgaaaagttc tgaaggtgct ttttcttcga cgaaaaggct ctgttccgcc cttgcttcga
     2221 tcctctctcc gggcgcaaaa acgtggatta tcccatattc tccatcgtat ccaggcttta
     2281 tcgagacttt gccagctctc atgcgtccta tggagagcgc taaaagctcg cctgaaagtc
     2341 caacagtttg aattgccttg atttcttctt cgctcttgtc catgagaaga gcaaactcgc
     2401 tgccagcttg ttctacgata ccgcggtaaa cagcggcaac ttttttggag ccgcttccgg
     2461 tgccgagaag ctcagcaagc agctctggca atggtatgag cgaccagtaa ggtcggcgat
     2521 ttgtgccttc ccagttcgca gggcagggag cattttcgtc cactgggcga tcggcaagct
     2581 ctgtgacccg gcgcatgact cccggcgtaa gaggcttgcc acagacaggg caacgcccat
     2641 caagttttgt gctttcttcg ggcgagagaa aaaccttgca agctcggtga ccgtcgtagt
     2701 gatattttcc ttcctggggg aaaaattcta ttgtgcctgc gaccgtccct gtgggcgtct
     2761 ctgcaacttt gcctgtgacg gtccctgtag ccgcctctgt gcccgagacc attcctgcag
     2821 acgctcctga gattttgctt tgcgcttcct ctgcggttga gcctcttttt gtgagcgcct
     2881 cgtaaaggcc agcgaaagat tcttccatgt cgataattgt cgcttcgcgg cccagattct
     2941 ctggcgaatg agcatccgaa ttggagataa tggcaaatga atcgagcgcg ctcaccgccc
     3001 agttcatagg agggttggaa gacaatcctg tctctattgc gccaattcga gagctgagat
     3061 ctccatagca ctcttcaatg gaatcgaagc ctgaacgagc tcccagcgca gagaaccacg
     3121 gtgtccagat atgggcaggt atgagaatcg aacgttcatc cgcatccaga agcaaagcga
     3181 agaggtcgcg cgaatctaga ccgagtatgg gtcgtccgtc ggaagatatg tttcctatcc
     3241 gtgcgagtcg cgcttgaaag gcggccgcag ccttaaaatc cggcaatatt acgacatgat
     3301 ggacttttct ggttttgcca tctctgttgt aaattgtgct tatttcgcca gagagcacaa
     3361 agtgcacatc agaatcgggc agttcagccg tcagcgcctc gctcgagtcg aattctttcc
     3421 taaccgaatc cttgagctta aagaatcccc cttcggtagg ctcgagctgc tccttgagtt
     3481 cgcggagcca agctgggtgc gtgcaatctc ctgtcccaac aagatttatc ccttttatcc
     3541 tggcccagcg ttcaatgtag ggcaaggtca gtttagggct cgtagcgcga gaatatcgag
     3601 agtgtatgtg aaggtccgca attattc
//
LOCUS       DAOOWY010000151         3626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_25676_length_3626_cov_28.190703, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000151 DAOOWY010000000
VERSION     DAOOWY010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25676_length_3626_cov_28.190703"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1006)
                     /locus_tag="PK105_08395"
     CDS             complement(182..1006)
                     /locus_tag="PK105_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOU61775.1"
                     /translation="MGIVPEKKPNPSIKRVIGVISGKGGVGKSMVASLLAQAFAERGL
                     QVAVLDADITGPSIPRLLGIDSFRGESDGEHLYPVENEEGIKVLSINLFNEQEDEPVI
                     WRGPLLAKAIDQFWSDTIWGELDYLIIDFPPGTSDVALTAFQTIPFSGLVVVATPQDY
                     VSMIVRKSINMASMLKAPVLGVVENMRTMICPHCGTEVALFDDGSQQGAQRMGLPILA
                     SLPWRKELAQTRALRWSTLSETVKKDADRLAGEVELALASVSSQPKDAESEIASKE"
     gene            1280..>3626
                     /locus_tag="PK105_08400"
     CDS             1280..>3626
                     /locus_tag="PK105_08400"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOU61776.1"
                     /translation="MTDKTEIPSLFGHDFLGWQVAPPIKSVVKRSGEVVSYDRHKIEK
                     AIEKAFIATNTSILDNQGNSKPILYTDLVEKKIRELIQTRHPNSIPAIEEIQDLVETV
                     LIEQGETAVAKAYILYRARHEAIRDTKKLMLDISATMNGYLARSDWRVNENANVNYSL
                     GGLILHNSGSVTANYWLTQIYPKDIAEAHRNADFHIHDLSMFSGYCAGWSLRDLIREG
                     LGGIPDKISSKPASHLSTLANQMVNFLGILQNEWAGAQAFSSFDTYLAPFVRADSLSD
                     KEIKQSLQSFVFGVNTPSRWGSQAPFTNITLDWTVPEDLRDKPAVVGGKELDFSYGDC
                     KPEMDRINRAFIELMLEGDASGRGFQYPIPTYNVTTDFDWDSPNAKLLFEMTAKYGTP
                     YFQNFINSDLNPADVRSMCCRLRLDKRELRKKGGGLFGADELTGSIGVVTINLPRIGF
                     LSRNEKEFFGRLDDLMEKAKISLVIKRKVIQRLLEAGLFPYTKRYLKTFENHFSTIGI
                     VGMNECLQNFFSPSITTATEEGRDFSLRILQFMKTRLADFQEETGDLFNLEATPAEST
                     SYRLAKHDKERYPDIITSGADEPYYTNSSQLPVGYTSDIFDALDLQEELQTVYTGGTV
                     FHVFLGEAISDWRACSQLVKTIAYSYRIPYFTISPTYSICPIHGYLAGEHFFCPLCAA
                     EERRKMEQKGKKMGEGAQILGTRTEVYSRIVGYYRSVKNWNAGKRAEYKERRVFDPDS
                     KPNERATCNSLAQRDCKAESDSPEQRDDSAESHSPEHYASGT"
BASE COUNT         1028 a          845 c          840 g          913 t
ORIGIN      
        1 cattctcaat ttccacaact acataatatg ttgctctgcc aaaatggcga gaaatggtga
       61 ttccgtcgtc ggatacaaat gcgattttca tactacctgt ttctccttct tataaaagag
      121 tttaccaaac aagagcgaat ttctgagctg attttttgga cgctgcgagg acaaggcatg
      181 attattcctt tgaagcgatc tcgctctctg cgtctttggg ttgagacgaa acagaagcta
      241 acgcaagctc gacttcgcca gcaaggcgat ctgcgtcttt ttttacagtt tcactaagag
      301 tagaccatcg gagcgctcgc gtttgcgcaa gctcttttct ccaaggaaga cttgcgagga
      361 ttggcaaacc catacgctgc gcaccttgct ggcttccatc gtcgaataag gcaacttctg
      421 tgccgcagtg gggacagatc atggtgcgca tgttctccac cacgccaagc accggcgcct
      481 tgagcatcga tgccatattg atcgacttgc gcacgatcat ggaaacataa tcctggggcg
      541 ttgcgacaac gacgaggcca gaaaaaggaa tagtctgaaa cgcggtgagc gcaacatccg
      601 aggttcctgg cggaaaatct ataatgagat agtcgagctc tccccagatc gtatcagacc
      661 agaattggtc gatcgcctta gccagcaatg gtccgcgcca gatcactggt tcgtcctctt
      721 gttcattaaa gagattgatg gagagcacct tgatcccttc ttcattttct acagggtaga
      781 gatgttcgcc atcggactca ccgcgaaagc tatcgatacc caaaaggcgc ggaatgcttg
      841 ggcctgtgat atctgcatcg agcacagcta cctgcaaacc tcgctctgca aatgcctgtg
      901 caagcagcga agcgaccatc gatttgccaa cgccaccctt accacttatt acaccgataa
      961 cccgctttat cgagggatta ggcttttttt cagggacaat gcccataaaa gactccttat
     1021 gcaaaatctt gttgtatggt ttcgagcata tgctaataat accgtagata tcgagaaaaa
     1081 ttcaagcctc cgcattgctt tatcagaaat ctagataaaa cgaatgaatg actatttgat
     1141 tgcagcttca tatacaaggt tcgtaacact aacgtttaat agctgcaact actttttgcc
     1201 tcttgtgctc accaaatgac caatatatat tgtgtctttg gtagcatggt acacaatata
     1261 tagtagaggt gatcgagata tgacagataa gaccgagata cccagtcttt ttgggcatga
     1321 ttttctagga tggcaagtgg ctcccccaat aaagagcgtg gttaagcgct ctggagaagt
     1381 ggtgagttac gataggcata aaatagagaa agccatagaa aaagcattta ttgcaacgaa
     1441 tacatcaatc cttgataatc aggggaatag caagcctatc ctatatacag acctcgtgga
     1501 aaagaagatt agagaactca tccagactcg ccatcctaac tccataccag cgatcgaaga
     1561 aattcaagat ctagtcgaaa ccgttcttat agaacaggga gaaaccgcag tagcaaaggc
     1621 ctacatcctc tacagagccc ggcatgaagc gattcgcgat acaaaaaagc tgatgttgga
     1681 catttcggca actatgaacg gatatctagc tcgatcggac tggcgcgtca atgagaatgc
     1741 caatgtcaat tattctcttg gtggactgat cttacataat tcgggaagtg taaccgcaaa
     1801 ttattggctc acacagatat atcctaagga tatcgctgaa gctcatcgaa atgctgattt
     1861 tcatattcat gatctttcta tgttttcagg ctactgcgca ggctggtctc ttagagatct
     1921 gattcgcgaa gggctgggcg gtattcccga caagatttcc tcaaagccag cgagtcatct
     1981 ttccactcta gccaatcaaa tggtgaattt tcttggtatt cttcagaatg aatgggcagg
     2041 agcacaggcc tttagctcct ttgataccta tctcgcccct tttgtgagag ccgacagcct
     2101 tagcgacaag gaaataaagc agagcctaca gagttttgtc tttggggtta atacgccatc
     2161 acgctggggg agtcaggctc cttttacgaa tatcacgctg gactggaccg taccggaaga
     2221 cctgcgtgac aaaccagccg ttgtaggagg aaaggagctt gatttctctt atggcgattg
     2281 caagccagaa atggatagga tcaacagggc ctttatcgaa ctcatgcttg aaggagatgc
     2341 atcgggcaga ggctttcaat atcccatacc cacatataat gtaacaaccg acttcgactg
     2401 ggactctcca aatgcgaagc tcttgttcga aatgactgct aaatatggca caccctattt
     2461 ccagaatttt atcaattcag atctaaatcc agcagatgta cgcagtatgt gctgtaggct
     2521 caggctcgat aaacgcgaat tgcggaaaaa aggcggtggg ctatttggag ccgatgagct
     2581 tacaggctcg ataggtgttg tgacaatcaa tctgcccaga attggttttc tttcacgaaa
     2641 cgaaaaggaa ttcttcggcc gactcgatga tctgatggaa aaggcaaaga taagtcttgt
     2701 cataaaacgc aaggttattc agcgcttact agaggccggc ctcttccctt atacaaaacg
     2761 ctatctaaag actttcgaaa accactttag cacgattggc attgtgggga tgaacgaatg
     2821 tcttcaaaat ttcttctctc cctctatcac aaccgctacg gaagaaggga gagatttttc
     2881 tcttaggatt ctgcaattca tgaagaccag actagcagat tttcaggaag agacgggcga
     2941 tctattcaac ctggaagcaa cgcctgctga atcgacctcc tataggcttg caaagcacga
     3001 caaggaaaga tatccggaca tcatcacctc tggcgcagat gagccctatt atacgaattc
     3061 ctcacaactc cctgttggat atacctctga tatcttcgat gcactcgacc tccaggagga
     3121 gttgcagaca gtctatacag gtggaacggt cttccatgta tttcttggtg aagccatctc
     3181 tgactggcga gcatgctctc agcttgtaaa gactatagcc tattcatata gaatccctta
     3241 ttttaccatc tctccaacct attccatttg tccgatacat ggctatctcg ccggagaaca
     3301 ctttttttgc ccactatgcg ccgcagaaga gcgtagaaaa atggagcaaa agggcaaaaa
     3361 gatgggcgag ggcgcccaga ttctcgggac aagaaccgaa gtttatagcc gcatagtcgg
     3421 gtattatcgt tcggtgaaga actggaatgc aggcaaaagg gcagaatata aggaaaggcg
     3481 cgtattcgat cccgactcaa aaccgaacga aagagcgacg tgcaatagcc tagcgcagag
     3541 ggattgcaag gcggagtctg atagccctga gcagagggat gactcggcgg agtctcatag
     3601 ccccgagcat tatgcaagcg ggacgc
//
LOCUS       DAOOWY010000152         3617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_25776_length_3617_cov_5.666480, whole genome shotgun sequence.
ACCESSION   DAOOWY010000152 DAOOWY010000000
VERSION     DAOOWY010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25776_length_3617_cov_5.666480"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1072
                     /locus_tag="PK105_08405"
     CDS             110..1072
                     /locus_tag="PK105_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61777.1"
                     /translation="MARYIFRRLLSLIPVLLGVSIIVFFLVRLIPGSALQMYLGTQVE
                     ATPEQMEELRRIFGEDKPIPVLYIEWLGRLLHGDLGYSLRTGRQVLPDILQRLPLSFE
                     LTVFALFLALLIGIPLGIIGAVKKNPILDIVIRITGLLGLSLPQFWLASLMVIAFSSF
                     RGFIPMGNYIPFIQAPLENIRMMLLPSLAIGIGLAAVIMRYMRNSMLEVMQMDYIRTA
                     QAKGLKRKIVISRHALKNAILPVITIAGFNTGYLLGGAIVIEEVFALPGMGRLVLYAI
                     YQRDYPLIQGIVLIIALMFVLINLLTDLLYAAIDPRIRLDQKET"
     gene            1077..1907
                     /locus_tag="PK105_08410"
     CDS             1077..1907
                     /locus_tag="PK105_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61778.1"
                     /translation="MSRIFKNSIAVIGMCIILFYVLVIVVSQFWLPFDPLDMDTINML
                     QPPSFETGHVLGTDEFGRDILSRIMKGAGISFLISISATALGGFIGTFMGIWSGFLGG
                     AWDNWIMRLADILFSFPSLLLAIFVMAVLGEHTYNVIIAISIGYIPQFARIARGSILS
                     IKNNEFIRASFASGASRSHILIHHLLPNIIMPIIVQSSLSLSVAILLESALSFLGLGV
                     QPPYPSWGNMLSSSRKVMILAPWTAIYPGLAIMLLVLGFNLLGDGLRDIMDPRLRNIQ
                     "
     gene            1916..>2301
                     /locus_tag="PK105_08415"
     CDS             1916..>2301
                     /locus_tag="PK105_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU61779.1"
                     /translation="MQDASKAILKIENLKTWFKMNQGTVKAVDGVSFDVHEGEIFGIV
                     GESGSGKSVTNLSVLRLIPVPPGFYAEGKIIFRDKDLLKVSEEEIRMVRGGSISMIFQ
                     DPLTSLNPLLKISLQIEEALQQHHSM"
     assembly_gap    2302..2401
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2427..3428
                     /locus_tag="PK105_08420"
     CDS             2427..3428
                     /locus_tag="PK105_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU61780.1"
                     /translation="MNEIAPLLQVKNLSVHFPVEKGIIFRKTIGWLKAVDDISFEIWP
                     GESVGLVGESGCGKSTTAMAIARLQSVNQGEIVFQGRNLLALSDSELCKARKDFQVIF
                     QDPYSSLDPRMTALDSIAEPMRIQAKRSGSSIGEKEITAHVYEIMDRVGLSRQFANRY
                     PHEFSGGQRQRIGIARALALSPKLVIADEPVSALDVSIQSQLLNLMKDLQGEFGLTYL
                     FIAHNLAVVSYFSDRIIVMYLGTIVEIASSIDLYREPFHPYTKALLSAVPIPDPKLER
                     ERSRILLKGDVPSPISTRMGCPFADRCSQAMDRCTRERPMLLPIGSSDHRVACYLYH"
BASE COUNT          962 a          711 c          784 g         1060 t
ORIGIN      
        1 ctttatttga tgccatatgc taagataaaa catatattat caagcctatt atgctaatca
       61 gaaaataggc agagtttaga agaaaaataa aaggaaaggc ttgagcctaa tggcaaggta
      121 tatatttcgc aggcttttat ctctgattcc agtgcttcta ggtgtctcta tcatagtttt
      181 tttccttgtt cgccttatac caggttcggc ccttcagatg tatcttggta cacaagtcga
      241 agctacgcct gagcagatgg aagaattgag gcgcatattt ggtgaagata aacccatacc
      301 agttctctat attgaatggc ttgggagact tttgcatggc gatcttggat attctttgag
      361 aacagggcgc caagttcttc cggatattct ccagcgcctc cctcttagtt ttgaacttac
      421 agtttttgcg cttttcttgg cgctcctaat cggcattcct ttagggatta tcggagcagt
      481 taagaaaaat cctattctcg atattgttat tcggattact gggttattag gattatcatt
      541 gcctcaattc tggcttgcat cccttatggt gattgcattc tccagctttc ggggattcat
      601 tccgatggga aactatatcc cattcattca agcacctttg gaaaacataa gaatgatgct
      661 cttgccttcg cttgcaattg gcattggtct tgcagccgta atcatgagat acatgcgcaa
      721 tagtatgctc gaagtcatgc agatggacta tatccgcact gcgcaagcaa aaggtctgaa
      781 gcggaagatt gtgatttctc gccacgccct caagaatgcg atattgcctg ttattactat
      841 tgctggcttc aatacaggat atcttttggg aggagcaatt gtcatagaag aagtatttgc
      901 tcttccgggg atgggaagac ttgtattata tgcaatctac caaagagatt atcccctaat
      961 tcaaggcata gtcctcataa ttgcccttat gtttgtgctt atcaatttgc ttaccgacct
     1021 cttgtatgca gcaattgatc cccgaattcg ccttgaccaa aaggagacat gaatcgatgt
     1081 ccagaatctt caagaattcg attgcagtta tcggaatgtg cattatcctt ttctatgttt
     1141 tggtaatagt tgtttcacag ttttggctac catttgatcc attggatatg gatacgatca
     1201 atatgcttca acctccttct tttgaaacag gtcatgtgct tggaacggat gaatttgggc
     1261 gagatatact ttcaagaatt atgaaaggtg ccggcatctc gtttttgatt tctatttctg
     1321 ctaccgcgct tggtggattc atcggaacat ttatgggcat atggtcggga tttcttggag
     1381 gagcttggga caattggata atgagattag ccgatattct attttccttt ccttcgcttt
     1441 tgttggccat attcgtcatg gcagttcttg gagaacatac atataatgtg ataattgcaa
     1501 ttagcatcgg atatatacct caatttgccc gaattgcaag aggttcgatc ctatcgataa
     1561 aaaataatga attcattagg gcttcttttg ccagtggggc aagcagatct catattctta
     1621 tccatcacct gctgcctaat ataattatgc caataatcgt tcagagttct ctatcattga
     1681 gcgtggcaat ccttcttgag tctgcattga gttttctggg tctcggcgtt caaccgccat
     1741 atccttcttg gggaaatatg ctaagcagct cacgcaaagt aatgatactc gctccatgga
     1801 ctgcaatata tccagggctc gccattatgt tgcttgtcct tggtttcaat ttgctaggcg
     1861 acggtctgcg agacataatg gatccaagac tgcggaatat acaatagagg gaatcatgca
     1921 agatgcaagt aaagcgatac taaagataga aaacctcaaa acttggttca agatgaatca
     1981 gggaacagta aaagccgtgg atggagtttc tttcgacgtt catgaagggg agatatttgg
     2041 catagtcggc gaatctggat caggcaaaag tgttaccaat ctttcggtgc tacgccttat
     2101 acccgttcca cccggcttct atgccgaagg aaagataatc tttagagaca aagatctttt
     2161 aaaggtgagc gaggaagaga tcagaatggt ccgcggtggc tcgatctcga tgattttcca
     2221 agaccctctt acttcgttga atcctcttct caagatctct cttcaaatag aggaagctct
     2281 acaacaacat cattcgatga annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nagaatttgc aaaaagggga taattcatga atgaaatagc tccccttctt caggtcaaga
     2461 atctatcggt gcattttcct gtagaaaaag gaattatttt tagaaagaca ataggctggc
     2521 tcaaggctgt cgatgacatc agttttgaaa tctggcctgg tgagtctgta ggacttgtgg
     2581 gagaatcagg ctgtggaaaa tcgacaaccg caatggcgat agcgcgctta cagtctgtga
     2641 atcagggcga gattgtattc caaggtcgaa atctgctggc gctctccgat tccgagcttt
     2701 gtaaggcgcg taaagatttt caggtcattt ttcaagatcc ttattcttca ctcgatcccc
     2761 gcatgactgc tctggattcc attgcggaac caatgagaat acaggcaaag agaagcggga
     2821 gttccatagg cgaaaaggaa ataaccgccc atgtctatga gattatggat cgcgtaggat
     2881 tatcaaggca atttgcaaat cgataccctc atgaattctc tggaggtcag cgccagcgca
     2941 ttggaattgc aagagccctt gctctatctc ctaaactggt aatagctgat gagcctgtaa
     3001 gcgcgcttga tgtatcgata caatcgcagc tcctcaatct aatgaaagat ttgcaaggag
     3061 aattcgggct gacctatttg tttattgccc ataatctagc tgttgtctca tatttcagtg
     3121 atcgcataat tgttatgtat ctcggaacta ttgttgaaat cgcttcctcg atcgacctat
     3181 atagagagcc atttcatccc tataccaaag ccttgctttc cgcggttccc attccagatc
     3241 caaaattgga gcgagagcgt tctagaattc tcttaaaggg agatgtccct tctccaatca
     3301 gcacaagaat gggttgccca tttgcagatc gctgttcgca ggcaatggat agatgcacaa
     3361 gggagcgccc aatgcttttg ccgattggat catcggatca tcgtgtggcc tgttatctat
     3421 atcattgatt tttgcatctt tgaccagaga aaagaaagcc tagctcgaag cgttatttag
     3481 atatggaaag ataagagaaa gataataagg agggcagaga aaaatggcgg atgattgttt
     3541 cattgccata ggcagcgaag atgatattat tgactgctca agaatcgcat gcaattcgga
     3601 aataggcata aaatatg
//
LOCUS       DAOOWY010000153         3554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_26384_length_3554_cov_14.189483, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000153 DAOOWY010000000
VERSION     DAOOWY010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26384_length_3554_cov_14.189483"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PK105_08425"
     CDS             <1..653
                     /locus_tag="PK105_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU61781.1"
                     /translation="NGSGKTTLFNCITGILKPNEGKIIFRGEDITGLKPNIIANKGIH
                     RTFQAGKLVSNLTVLENIMCGVQDPLSKSLKDTMIRLPFTQSKREKEIQEKAERIAEF
                     VGLVDSINRWASDLVWTERQLVQIARALINEPLLLLLDEPASGMGSFEIEKMEEIIQK
                     IREMGITIVVVSHEVKMLMNLSEIVTVLSFGQKIAEDIPEVIQKDPRVLEAYLGTR"
     gene            682..1380
                     /locus_tag="PK105_08430"
     CDS             682..1380
                     /locus_tag="PK105_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61782.1"
                     /translation="MLIVDEISVSYGHIQALKKISIEVYPQEIVVLIGANGAGKTTLL
                     SSILGVRAPSSGKVVFEGKRIDKNSVDKNVRAGLCLVPEGRGVFASMSVLDNLLLGAH
                     HNLANKDKNLARVYEWFPILRKRKDQIAKTLSGGERQMLAIARAMMSDPKLIMIDEPS
                     LGLAPIIVNDIFDILVTLNKEGYTVLLAEQNAFKALKCAHRGYVLETGTISLSGPSSQ
                     LLNDPGVKAAYLGA"
     gene            1383..2267
                     /locus_tag="PK105_08435"
     CDS             1383..2267
                     /locus_tag="PK105_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61783.1"
                     /translation="MQVFIAQILNGLSIGSVYMLLATAFNLLLLVAMIIHFSYPQVVV
                     FCMYIIWLVLKITNNNVFFGILAGVSSAILFNIVSAPLFQKIMKNREEVDINATMVLS
                     MAIGWIITDVLSHAFNNGLPIQFPEEITGSKPLFRVGLISILPGQVLALTIGIIVVAG
                     FFLLLYKTKTGRAFRAIAEDPAGAKLVGIPLVKTGFQSYALSGALGGLTAVLLIMLFH
                     SASPGLGEQVSHKVLAVSIVAGLGNLVGGLVVGLALGILEALVQGYISGNWSNAIAFV
                     VMLVVILVKPRGLFGVKI"
     gene            2277..3182
                     /locus_tag="PK105_08440"
     CDS             2277..3182
                     /locus_tag="PK105_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU61784.1"
                     /translation="MPLSLYYFLSITAVNILMSWALYVPYRVTQLHFITIANMMISGY
                     LAGYLVLHAHVPFVWALLIGFAVGGIIGFIVSLFIGDAPTFSVVIVGFTSIYITRTLV
                     ENLPIIGGTMGMFGLPNIGGNPVAHRWIILCILYGLVILCGFLIYRFDHSSIGRAASA
                     VFVDKELASSLGIDIKKLGMLLQTFSCMLAGGVGVLYGFIYRSFHLDFFSFALIGVFM
                     TVLFVGGYSTQWGPLITAPILYGLPLFFPPEIQSWRIVIYGLLLILVLELKPEGFITR
                     KFVHSLGLSFSQKRGKEIYIKEDRQ"
     gene            3179..>3554
                     /locus_tag="PK105_08445"
     CDS             3179..>3554
                     /locus_tag="PK105_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61785.1"
                     /translation="MKRLVAALLLIIAAVSFGFSQTKVVVDEWKIPFLNCLTGPIAAI
                     GEFLQWGANYAANEINAAGGIAGKPVRVIPVDTGNDPQKGSVEMARIVKDSLVAMGPV
                     PEPVILAAMPIAVENQMMSITAT"
BASE COUNT          962 a          667 c          836 g         1089 t
ORIGIN      
        1 caaatggctc tggaaagacc actcttttca attgcataac agggatactt aagcccaatg
       61 aagggaaaat tatttttagg ggcgaagaca ttacgggtct aaagccaaat attattgcga
      121 ataaagggat ccatcggaca tttcaagcag gcaaattggt ttcgaacctt acagtgctcg
      181 agaatataat gtgtggcgtt caagaccctt tatccaaatc tttgaaggat acaatgattc
      241 gattgccttt tacccaatcc aagagagaaa aggaaatcca agaaaaggct gaaaggatag
      301 ccgagtttgt gggacttgtc gattcaatta atagatgggc tagtgatctt gtttggacag
      361 agcgacagct tgttcaaata gcgcgcgcgc ttatcaatga accgttactg ctcttgcttg
      421 atgaacctgc atcggggatg ggatcgtttg aaatcgaaaa aatggaagag attattcaga
      481 aaatacgaga aatgggaata acgatcgtag tggtgagtca tgaggtaaaa atgttaatga
      541 atctatccga aatcgttacc gtgctcagct ttgggcaaaa aattgccgag gatatcccag
      601 aggttattca aaaggatcct cgagtattgg aggcatacct tggaacaaga tagcaccttg
      661 gctagcgaaa aaaaggcgcc tatgctcatt gtcgacgaaa tctcagtatc atatggacat
      721 atacaagcct taaagaaaat atctatcgaa gtatatccac aagaaattgt tgtattgatt
      781 ggagctaatg gagcaggaaa gacgacgcta ctgtcatcta tactcggtgt aagagcaccg
      841 agttcaggga aagtggtctt tgaaggaaag aggattgaca aaaactctgt ggataaaaat
      901 gtcagggctg gtctttgctt ggtccccgaa ggacgaggag ttttcgcctc gatgtcggta
      961 ttggacaatc tgttgcttgg agctcatcac aaccttgcaa acaaagataa gaaccttgct
     1021 cgtgtttatg aatggttccc tattctaaga aaaagaaagg atcagatagc aaaaacactt
     1081 tcaggtggcg agaggcagat gcttgccata gcgagagcga tgatgtcaga tcccaaattg
     1141 ataatgatag acgagccatc gcttgggctg gcgcctatta ttgtcaatga tatttttgat
     1201 attttggtca ctttgaataa agaaggatat acggtgctat tagctgagca gaacgcattc
     1261 aaagcgctta agtgcgctca tagaggatat gttctcgaaa caggtacgat ttcgctctca
     1321 ggcccctctt ctcagctttt aaatgatccc ggtgtaaaag cagcctatct aggagcatga
     1381 atatgcaagt ttttattgcg caaatattga acggtctctc aattggaagt gtatatatgc
     1441 ttttggctac agcttttaat cttcttcttc ttgtagctat gatcattcat ttttcctatc
     1501 ctcaagttgt tgtattttgt atgtacatta tctggttagt acttaaaatt accaataaca
     1561 atgtattttt tggaatcttg gcgggagttt cttctgcaat attattcaat attgtcagtg
     1621 cgccgctttt tcaaaaaatc atgaaaaaca gagaagaagt cgatattaac gcaacaatgg
     1681 ttctctctat ggccataggg tggataatta ctgatgttct ttcacacgcc ttcaataacg
     1741 gacttccgat ccaatttcca gaagaaatta ctggaagtaa gcctttgttc agagttggct
     1801 tgatcagcat cctgcccggt caggtcttgg cattgactat cggaatcatt gttgtagccg
     1861 gattttttct tctgctctac aaaaccaaga ccggaagggc tttcagagca attgcggaag
     1921 atccggctgg tgcaaaactt gtaggtattc ctttggtaaa aacgggattc caaagctatg
     1981 cgctctcggg ggctttaggc gggctgactg cggtcttact gataatgctt tttcattctg
     2041 catctccggg cctaggagag caggtttcac ataaggtctt ggcagtatct atagtcgcgg
     2101 gtcttggcaa tctggtcggt ggtctcgttg tagggttagc tctcggtatt cttgaagcgc
     2161 ttgtccaggg atatatctct ggaaattggt caaatgctat tgcatttgta gtcatgctcg
     2221 tagtgatttt agtcaaacca agaggcttgt ttggcgttaa gatataggag gagctcatgc
     2281 cactttcgct ctattacttt ctttcaatca cagctgtgaa tatattgatg agttgggctc
     2341 tctatgtgcc atatagagtc acgcagttgc attttattac catcgcgaac atgatgattt
     2401 ctgggtatct tgctggatat ttggttttac atgctcatgt tccttttgta tgggctcttc
     2461 tgataggttt tgcagtaggg ggcatcatag gctttattgt atccttattc atcggcgatg
     2521 ccccaacatt ttccgttgtc attgtaggat ttacttctat ttatattaca agaacacttg
     2581 tagagaattt gccgataatc ggtggaacga tgggaatgtt tggccttccc aatataggcg
     2641 gtaatccggt ggcgcatcga tggataatcc tatgcatctt gtatgggctc gttattctat
     2701 gcggctttct tatctatcga tttgatcatt ccagcattgg gcgcgcagct tctgccgttt
     2761 ttgtagataa agaattggca tcttcgcttg gaatagatat taagaagctt ggtatgctac
     2821 ttcaaacatt cagttgtatg cttgccggcg gagtaggcgt tctatatggc tttatctata
     2881 gaagctttca cctggatttc ttcagttttg ccttgatagg tgtctttatg acggttcttt
     2941 tcgtgggtgg atattccact caatggggtc cgctcattac cgctccaatt ctttatggtt
     3001 tgccgttatt ttttccccca gaaattcaat cttggcgtat agtaatatat ggattgctcc
     3061 ttattcttgt tctagaattg aaaccagaag gcttcataac caggaagttt gttcattcct
     3121 tgggtttatc cttctcgcaa aagcgaggaa aggaaatata tattaaggag gacaggcaat
     3181 gaaaagactc gttgctgcat tgttgctgat tattgcggct gtgagttttg gctttagcca
     3241 aacaaaagtt gtagtggatg aatggaaaat tccatttctc aactgtctaa ctggccctat
     3301 tgcggcaatt ggagaattcc tgcaatgggg ggcaaattac gcggccaatg aaataaatgc
     3361 tgcaggaggt attgcaggaa agcctgtcag agtcattcct gtcgatacag gcaatgatcc
     3421 tcagaaaggc tcagttgaga tggcacgcat tgtcaaggat agtcttgtgg ccatgggacc
     3481 tgttccagag cctgtaatct tggcagccat gccgatagca gtagaaaatc agatgatgtc
     3541 cataactgcg acga
//
LOCUS       DAOOWY010000154         3541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_26512_length_3541_cov_34.518072, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000154 DAOOWY010000000
VERSION     DAOOWY010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26512_length_3541_cov_34.518072"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PK105_08450"
     CDS             <1..573
                     /locus_tag="PK105_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase"
                     /protein_id="HOU61786.1"
                     /translation="LLYGFNRRMEKDQPLSAPNDVRRYFVTPKESGELCLMSALLGEN
                     RDLFFPKLDPNENLLTFSEIAVRYLASRGYEAVECASEDEARSRVAELKAQRKWPVYF
                     FTSDTTGEKDFEEFYTEHETLDMSRFASIGVIKNEPEYEATALTRFEDAIHAMRSRGV
                     WTRQELIDLFNATIPEFAHKETGKFLDGRM"
     gene            573..1256
                     /locus_tag="PK105_08455"
     CDS             573..1256
                     /locus_tag="PK105_08455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU61787.1"
                     /translation="MLQAALIRCFDIIFSALAIVVLFPFMIPIMIALKLTGEHYIFYL
                     QPRVGKGGKDFKVLKFATMLKDSPNLPGGVLTQKNDPRILPMGKFLRKTKINELPQLA
                     NIFIGQMSVVGPRPQARQHYELYSDTVKREIDKVPPGLSGIASIVFRDEEALLDHVDA
                     DRDHFHDTIIAPYKGQLEVWWVQHRTLANYFKIIILTIIVLFAPHSRLYQKWFKELPP
                     VPKELQEVL"
     gene            1370..2644
                     /locus_tag="PK105_08460"
     CDS             1370..2644
                     /locus_tag="PK105_08460"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOU61788.1"
                     /translation="MSDQKLEMEGQPVQGSLEKSKDSDEISLLDLVAVLWKRKWLVIA
                     ITGLVAFGSLAYALGSLLLPPDKSYLPNVYTPKATMLISSGTSSSLSSLLSSSGLSGL
                     AGMAGVSAGGNANQQLAKVLATSNTTLDRLNERFNFAEHYKMKEPKVAEVRKAISNNL
                     SATIDEKSNIFTISFTDKDPAFAKEVVDETVNILSERFAQLSGSQAIQQKALLEKRLA
                     DVDAAISTLEAQVKEFQKKYGVVQVEALATEQITILARLRSELILKEMEIENYKKVAR
                     INDPMLTQLNNERDALLAKIKEIETGTGSGSKVMPSQQQLPTIAFEYAKLERDLAVQT
                     ELFKILTQQYEVAKFNAAGQEPVFQIIEMAEVPDMKSGPARSTICIVATLAGFFVSIL
                     LVFASESISKIRRDPEAVARFKAISKGEEPGI"
     gene            2834..3445
                     /locus_tag="PK105_08465"
     CDS             2834..3445
                     /locus_tag="PK105_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU61789.1"
                     /translation="MKRQITALLIFATTLCVLIGSCSNKIPEVVSVFGVKLDKSSMGL
                     AVGESRKLTATVEPKNATDKGVSWESDNDLAAKVDQDGNVTGISKGRAKITVITDDSG
                     HKATCDVGVVEIPVEKVTLDHDKIELNIDNPYQFSWTIFPDGASIKEVEFSSSDESVV
                     RCADKPLGLIYPTKIGVATITVTTVGTDKDDKQCKAECEVTVK"
BASE COUNT          972 a          890 c          859 g          820 t
ORIGIN      
        1 ctgctctatg gctttaatcg tcgaatggaa aaagaccagc cgctctcagc gccgaacgat
       61 gtgcgccgct attttgtcac gcccaaagaa tcaggcgagc tctgcctcat gtccgcgctc
      121 ctcggtgaga atcgcgatct cttttttccc aagctcgatc caaacgaaaa cctccttacc
      181 tttagtgaga ttgccgtgcg ttaccttgca agccgcggct atgaagccgt ggaatgtgca
      241 agcgaagacg aagcccgctc tcgagtggcg gagctcaaag cacagcgcaa gtggcctgtc
      301 tatttcttta caagcgacac caccggcgaa aaagactttg aggaattcta taccgagcac
      361 gaaaccctcg acatgagccg ctttgcttcg atcggcgtca ttaaaaacga acccgagtac
      421 gaagccacag cgctgactcg ctttgaagac gcgatccatg ccatgcggag ccgtggcgta
      481 tggacccgcc aggagctcat cgatcttttt aatgccacta tcccagagtt tgcacataaa
      541 gagaccggca aattcctgga cgggagaatg tgatgctgca ggccgcgctc attcgttgtt
      601 tcgatataat cttctcggca cttgctatcg tggtgctttt cccatttatg attcccatca
      661 tgattgcact caagctcacc ggggagcact acatctttta cctccagccg cgcgttggca
      721 aaggcggcaa agacttcaag gtccttaagt tcgccaccat gctcaaagat agtcccaatc
      781 tcccgggcgg cgtgctcacc cagaaaaacg atccacgcat cctccccatg ggcaaatttc
      841 tgcgcaagac caagatcaac gagttgcccc agcttgccaa tatctttatt ggccagatga
      901 gcgtggttgg ccctaggccc caggcgcgcc agcactacga gctctattcg gacacggtta
      961 agcgcgagat cgacaaagtc ccgccaggcc tctccggtat cgcctccatc gtcttccgcg
     1021 acgaagaagc cctgctcgac catgtcgatg ctgaccgcga tcacttccac gacaccatta
     1081 tcgcccccta caaaggccag ctcgaagtct ggtgggttca gcatcgcacc cttgctaact
     1141 atttcaaaat catcatcctt accatcatcg tgctctttgc gccccacagc aggctctatc
     1201 agaaatggtt caaggagctg ccccctgtgc ccaaagagct acaagaagtc ttatgaaaaa
     1261 tcctgttaat cctgcatcat cctgccatcc cgccataggt ttttaatgag cgccacgcta
     1321 gtggtgcagg tcgataattt gatatagtag cgaatgcaga gaggaaggaa tgtcagatca
     1381 gaaattagaa atggaaggtc agcctgtgca gggcagccta gagaaaagca aagatagcga
     1441 tgagatcagc ttactcgacc tcgttgcggt gctctggaag cgcaagtggc tcgtcattgc
     1501 cataaccggc cttgtagcct tcggctcgct tgcctatgcg ctgggctcgc tgcttctgcc
     1561 tcccgacaag agctatctgc caaatgtcta cacgcctaag gcaacaatgc tcatttcatc
     1621 ggggacctca agcagccttt cgtcactgct atcatcgtcg ggcctctcgg gccttgcggg
     1681 catggccggt gtttcggcag gaggtaatgc gaatcagcag cttgcaaaag tattggccac
     1741 ctccaacaca acgctcgata ggctcaacga aaggttcaat ttcgccgagc attacaagat
     1801 gaaggagcca aaggtagcag aagtaagaaa agctatctcc aataatctgt cggccactat
     1861 tgatgaaaag tcgaatatct tcacgatctc atttaccgat aaggatccgg cctttgccaa
     1921 agaggtcgtc gatgagaccg tcaatattct ctccgagcgt tttgcgcagc tgagcggtag
     1981 ccaggcaatt cagcagaagg ctcttctgga aaagaggctt gcggatgtcg atgccgctat
     2041 cagcactctc gaggctcagg tcaaagaatt ccagaagaaa tacggcgtcg tccaggtcga
     2101 ggctctcgcc acagagcaaa taacaattct cgcgcgcctt cggtcggagc tcatcttaaa
     2161 ggagatggag atcgaaaatt acaagaaagt cgcgcggatc aacgatccca tgctcaccca
     2221 gctcaacaac gagcgcgatg cgctgctcgc caaaatcaag gaaatcgaga caggcaccgg
     2281 ttcaggctcg aaggttatgc caagccagca gcagctgccc accattgctt ttgaatatgc
     2341 caagctcgag cgcgatcttg cggtgcagac cgagctcttc aagatactca cccaacagta
     2401 cgaagtcgcc aagttcaacg ctgcaggcca ggagccagtc ttccagatca tcgagatggc
     2461 agaggtgcca gacatgaagt cgggcccagc gcgcagcacg atctgcattg tggcgaccct
     2521 tgcggggttc ttcgtgtcta tcctccttgt ctttgcctca gaatctatct cgaaaatccg
     2581 ccgcgaccca gaagcggtag ccagattcaa agcgataagc aaaggcgagg aacctgggat
     2641 atagcttggg taagccaata tcaatgaaca ggcttatgta atattttatt gtgaaaatgg
     2701 aaactctcaa gaagctataa gagctagatt cctaatgctc ttaactcatg cccatttttg
     2761 agaaaaattt atataaaact ccataatagt cgataataaa ataaaattcg catagataat
     2821 tggagggcat gatatgaaaa gacagatcac ggcgctctta atatttgcta ctacattatg
     2881 tgttcttata gggagctgtt cgaataagat accagaagta gtgagcgttt ttggagtgaa
     2941 acttgacaaa agcagcatgg ggctagcagt aggagagtca aggaagctaa ccgcaactgt
     3001 agaaccaaaa aatgctacag ataaaggtgt gagttgggaa tcggataatg atctagcagc
     3061 aaaggtagat caggatggca atgtgactgg aataagtaag ggtagagcaa agataacagt
     3121 gataacagac gatagcggtc ataaagccac atgtgatgta ggtgtagtgg aaatacctgt
     3181 agaaaaagtc actcttgatc atgataagat tgaattgaac atagataatc catatcagtt
     3241 tagttggaca atatttcctg atggggcttc gattaaagaa gtggaattct cctctagtga
     3301 tgaaagcgta gtgagatgtg ccgataagcc attgggtctt atttatccca caaaaattgg
     3361 tgtcgccact attacggtta caaccgtggg tacagacaaa gacgacaagc aatgcaaggc
     3421 cgaatgtgaa gttaccgtaa agtaagagtc ttatcgaata tttttctcta tatgatgtaa
     3481 tccctgtcgc gctagacgat atggatatag tgcgataggt tttctattca ctatcgatct
     3541 c
//
LOCUS       DAOOWY010000155         3461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_27310_length_3461_cov_5.454492, whole genome shotgun sequence.
ACCESSION   DAOOWY010000155 DAOOWY010000000
VERSION     DAOOWY010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27310_length_3461_cov_5.454492"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PK105_08470"
     CDS             complement(<1..1185)
                     /locus_tag="PK105_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU61790.1"
                     /translation="MRENTRLSVAHLVRDPVLLFLVILVFVGLFLFIIFPLCKIFAYS
                     VTDNDGNLSFQAAWDMLKSRSYLQPLKNSMMLGIATGILATIIGYVFAYALTRVDMPL
                     KGFFKTIATIPIISPPFILSLSMIFLFGRNGLITRRLLGIEDANVYGMHSLVVVQTIS
                     FFPIAYLTLTGTLSKLNPAVEDAALNLGASKARIFRTVTLPLSVPGILSSFLLVFIQS
                     MEDFSNPAVISGSFSTLAVEAYRTITGMYDMRGGSLMALLLLIPTLIAFLLQKYWLSG
                     KSFVTVTGKPTVSQQQSKDPKMIWPLFAFCMLVALVILLFYGTVLVGAFVKVWGINFS
                     FTWAHFKYVMTLGWIPLRNSVVLAMASTPIAGLLGMIIAFLVVRKEFPGRRAMEFISM
                     LTF"
     gene            complement(1300..2331)
                     /locus_tag="PK105_08475"
     CDS             complement(1300..2331)
                     /locus_tag="PK105_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61791.1"
                     /translation="MRKIFLSVLVLFCLLPLMSTSAQATPDYKSLTVYTALPESELPT
                     YFSQFEKDTGIKIQYVRLSAGELLARVRAEKNNPQATVWFGGSYDNFVPAISEGLLEA
                     YQSPELVNIPKVYWDVKGYANPFYVGAIGFACNTDWFKKKGLAYPTSWDDLLKPEFKG
                     QISMAHPSTSGTSYTILATIVQMRGEEATWKYFSALNQNIRQYTKSGTTPPMDVGLGE
                     AAIGITFSHDGLKPAFEGYPVALSFPKDGTGYEIGCMALIKNGPSKETSNGKRFIDWM
                     LSKRGQDLFETSKSFRIPVNSQATPPKGSISVDSLKVIDYDPIWAGQNRNRLIEQFNK
                     VVAAASNLK"
     gene            2520..3407
                     /locus_tag="PK105_08480"
     CDS             2520..3407
                     /locus_tag="PK105_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU61792.1"
                     /translation="MQYDITMMGHISKDIIIDESGNESKLYGGALLYSSIAAARAGAH
                     VLAITKAAPEDFCALDIAKEHSGIQIIAIQSPKTTSIRNVFLSADHERRETCLLSRAE
                     SFSAADIPLNAETKIIDLAGLFVGELPDNLIEELAPRGAIAVDAQGLLREAQPNGRML
                     FRDWNNKHRYMPFVSYFKADAAEAEILTGLTDREEAAKKIAAWGRDSEGKEPEVMITY
                     NTEVIVFVDGKIYRAPYTPSNLSGRTGRGDTTFSAYLAWRLSHDPHESVRFAAALCSI
                     KMEKPGPFSGTLEDVFARM"
BASE COUNT          990 a          780 c          834 g          857 t
ORIGIN      
        1 aaaagtcagc atggagataa actccattgc tcgtctgccg gggaattcct tgcgtacaac
       61 caggaaagca atgatcattc caagaagccc agcaatcggc gtagacgcca tggcgagcac
      121 aacggaattt ctcagcggaa tccaaccgag agtcattacg tatttgaagt gcgcccaggt
      181 aaagctaaag tttatgcccc agaccttgac aaaagcgccc acgagaaccg ttccatagaa
      241 caagagaata accagcgcaa ccagcatgca gaatgcaaag aggggccata tcatcttagg
      301 atccttgctc tgctgctgcg atacagtggg ttttcccgtg actgttacaa aagacttgcc
      361 cgatagccaa tatttctgca acagaaaggc gatcagggtc ggtatgagta gcaagagcgc
      421 catcaaagaa ccgcctctca tatcgtacat gcctgtaatg gttctgtacg cctctacagc
      481 aagagtggag aaacttcccg aaatgaccgc tggattgctg aaatcctcca tggattgaat
      541 gaacacaagc aaaaaagagc tgaggatacc cggcaccgaa agcggcaaag ttacagttcg
      601 gaaaattcga gccttagagg caccaagatt cagtgctgca tcttccaccg ctggatttaa
      661 tttcgaaaga gtgccggtca gcgtaaggta ggctatcgga aaaaaagaaa ttgtctggac
      721 aacaacaagg ctatgcattc catagacatt tgcgtcttcg atgcctaaaa gcctgcgagt
      781 tatcagccca tttcgcccaa aaagaaagat catcgataga gaaagaataa aaggcggaga
      841 gattatgggg atagtcgcta ttgtcttgaa aaatcccttc agtggcatat ctacacgagt
      901 cagggcatat gcaaagacat atccaatgat agttgcgaga atacctgtcg caatacccaa
      961 catcatagaa ttcttgagcg gttggagata tgagcgagat tttaacatat cccaagcggc
     1021 ttgaaaactc aaattgccat cattatccgt tacactatat gcaaatatct tgcatagggg
     1081 aaatattata aaaagaaaaa gccctacgaa taccaggatt actagaaata aaagtaccgg
     1141 atcacgcaca agatgcgcta ccgaaaggcg agtgttttct ctcattaaaa tctcaggttc
     1201 ctttggaatg aagattgtgc agtcatatcg cttttgcaca agaggtgcgc aaagaaaatt
     1261 tcgcgcacct ccataaatgc tcaatctacc tgatcaattc tacttcaggt tgcttgccgc
     1321 cgcgactact ttattgaatt gctcgattag gcgatttctg ttctgaccag cccatatggg
     1381 atcatagtcg atgaccttga gactgtcgac cgaaattgag cccttgggtg gagttgcctg
     1441 tgaatttacc ggtattctaa aggatttaga agtctcgaag aggtcttgcc cgcgctttga
     1501 caacatccag tcgataaatc gtttgccatt tgatgtttct tttgatgggc cattctttat
     1561 cagcgccatg cagccaattt cgtaacctgt gccatcttta gggaaggata gggcaacagg
     1621 gtagccttcg aatgccggtt tgaggccatc atgagagaat gtgattccaa tggccgcttc
     1681 tccgaggcct acatccatag gaggagtcgt ccccgatttg gtatactgac gaatattctg
     1741 attgagagcg gaaaagtatt tccaggtggc ttcttcacca cgcatctgga caatagttgc
     1801 aaggattgta taggaagttc ctgaagtgct tgggtgagcc atcgagattt gacccttgaa
     1861 ttcgggcttc aagagatcat cccaagaggt agggtaggcc aagcctttct tcttgaacca
     1921 atcggtgttg catgcaaagc caattgctcc cacatagaag ggatttgcat agccctttac
     1981 gtcccagtac accttcggaa tgtttacaag ttcaggagac tgataggctt cgaggagacc
     2041 ctcggagatt gcgggtacaa agttgtcgta ggaaccgcca aaccagacgg tagcctgggg
     2101 attgttcttt tccgctctta cacgagcaag cagctcgcca gcagagagtc tgacatattg
     2161 gatcttgatt ccagtatctt tttcgaactg gctgaaatat gtcgggagct cggattccgg
     2221 cagagccgta taaacggtta gtgacttgta atctggtgtt gcctgcgcgc ttgttgacat
     2281 caagggcaat aggcagaaca gcactagtac cgatagaaag atcttgcgca tagattgcct
     2341 cctttcaagt tcctcgtcta ttatagaaca atattataga tcaaatttag caaaatgcaa
     2401 ctgaaatcgt ttgcaggatg gcaggatggt aagaactatt ttatatattt gctgtacaga
     2461 ggcaaaggag atccataaga ggagatttta taaagaagaa tcctggaagg agaaagataa
     2521 tgcaatacga tataactatg atgggtcata tttcaaaaga tataataatc gacgagtctg
     2581 ggaatgaaag caagctttat ggtggagcac ttctgtattc atcaatcgct gctgcgcgag
     2641 ccggagccca tgtactcgcc ataactaagg ccgctccgga ggatttttgc gcattagata
     2701 tagccaagga gcacagcggc atacaaatca tagctatcca gagccccaaa acgacatcga
     2761 ttcgcaatgt atttctgtct gcggatcatg aacgaagaga gacctgtctc ttatctcggg
     2821 cagaatcatt ctcggcggct gatatccctt tgaacgcaga gacgaagata atcgatctgg
     2881 caggactatt tgtaggcgaa ttgcctgata atctaataga agagcttgca ccccgtgggg
     2941 ctattgcggt cgatgcccag ggtcttcttc gcgaggctca gcccaatggc aggatgctgt
     3001 ttcgcgattg gaataacaaa catcgctata tgccttttgt gagctacttc aaagccgatg
     3061 ccgcggaagc ggagatcctc acaggtctta ccgacagaga agaagccgca aagaaaatag
     3121 cggcctgggg cagagactca gagggcaagg aaccagaagt catgatcaca tataataccg
     3181 aagtcatcgt ttttgtggat ggaaagatct atcgtgcccc atatacgcct tcgaatctct
     3241 cgggaagaac ggggcggggc gacaccacct tttctgccta tcttgcctgg cgcctcagcc
     3301 acgatccaca tgaatcggtc cgatttgcag cggctctttg ctcaataaaa atggaaaagc
     3361 ccgggccttt ctccggaacg ctcgaagatg tgtttgcgcg tatgtaaata gccgatatct
     3421 cttgcacctt gccgctctat attctttgat gggcttttcg c
//
LOCUS       DAOOWY010000156         3450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_27435_length_3450_cov_18.200884, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000156 DAOOWY010000000
VERSION     DAOOWY010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27435_length_3450_cov_18.200884"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1583
                     /gene="secD"
                     /locus_tag="PK105_08485"
     CDS             3..1583
                     /gene="secD"
                     /locus_tag="PK105_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOU61793.1"
                     /translation="MTYLIISDLKERAAAGDTTDLSNQKNYKVAIDSAKRAYSLAKKP
                     APRTWDAVAILSAFSSERSMFDTIESRYREYVLKLKNVHSNAVQLGLDLAGGMSAVVQ
                     ADLKGLSEKLGHELSQAEKEDAMKRAVEILNSRIDKFGLTEPVIRRQGEDQIYIEIPG
                     TPDPERINSIIMGKGNLAFYIADLEASAAVNSYLSTNPVGIDEETMTVDKPGLVPEGK
                     IVRKVYKKDSYGLDEFTGEYLVLEGKPGLDGSHIVSATVSSDPITGQPETNFVLDKEG
                     GDIFYELTSANVGKTMAVVLDDRVKSYARIQEPIRESVRITGFSAEEAEALALLLRTA
                     ALPISLSVVSQQSIGASLGEDAISQGQNAIIVGILSIFVFMFIYYKWAGINATIAQIL
                     NVYLMLSVLTAFKLTLTLPSIAGFVLTTGMAVDANVIIFERIKEEMKAGKSRAAAIHA
                     GFDKAFWAVMDSNITTIIAALFLAQLGTGPIQGFAVSLAIGNITSLFTSLFVSRLIFD
                     FETDVLKLKTVSISWRIR"
     gene            1583..2833
                     /gene="secF"
                     /locus_tag="PK105_08490"
     CDS             1583..2833
                     /gene="secF"
                     /locus_tag="PK105_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOU61794.1"
                     /translation="MKSIIKFSKFFLPAIIISLSIIAFGLAGFFTKGLNLGVDFQAGI
                     NETIQLAYPAGQVVFEGKGNAELTIGESQLTLVFSGAEAQKRTVLFDYKTYSTLADIK
                     AALEKEQGIRVDLAEGSDSLPSNLLVPTYQGNSLLSLNPVKLYRAPKDDSELYASIDI
                     VRDALSPLGDITVQTINPPSSQRFLIRVRDNGEDPQFMTNVPQTIRNLIEDKVGADRL
                     VVVKADYVGPQYSETLSRQSIWLVLMTLVLILVYSTIRFKIEYAIAAVAAIAHDALVM
                     IAFIVWTRMEFNTTTIAAILTILGYSINDTIVQFDRVREERKLHPSEPFVDVLDRALT
                     LTLGRSIITTLTTMLAVLALYFFTSGSIKDFALALLVGMVSGVYSTIFIASAIVLFLE
                     NKKTKRRAKTIKEKTAPAGALSKQ"
     gene            2929..3000
                     /locus_tag="PK105_08495"
     tRNA            2929..3000
                     /locus_tag="PK105_08495"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2961..2963,aa:Cys,seq:gca)
BASE COUNT          963 a          753 c          825 g          909 t
ORIGIN      
        1 ggatgaccta tctcataatc agcgatctaa aagaaagagc agctgcgggc gatactacgg
       61 atctctctaa ccagaagaac tacaaggttg caatagattc ggcaaaaagg gcttattctt
      121 tagccaagaa accagcaccc aggacatggg atgcggtagc cattttatcg gctttttcca
      181 gcgagcgctc catgttcgat acgatcgaat ctcgctatag agaatacgtg ttgaaactca
      241 agaatgtgca ctcgaatgcg gtgcagcttg gactcgacct tgccggcggt atgagtgcag
      301 ttgtccaggc tgatctcaag ggattgtcgg agaaactagg gcatgagcta tcccaggccg
      361 aaaaagaaga cgccatgaag cgcgcggtgg aaatcctcaa ttcgcgcatc gataaatttg
      421 gccttacaga accagtcatt cgtcgccagg gtgaagatca gatctatatt gaaattccag
      481 ggactccaga tccagagcgc ataaattcga ttattatggg taaagggaac ctagccttct
      541 atattgcgga tttggaagcc tctgcggccg tgaattccta tctttctact aatcccgtag
      601 gcatcgacga agagactatg acagtggata agccaggcct cgttcccgaa ggcaaaatcg
      661 tccgaaaggt ctacaagaaa gacagctatg gtctcgatga attcaccggc gaatatcttg
      721 ttctcgaagg taagcctggt ctggatggca gccatatcgt aagcgcaaca gtctcttccg
      781 atccgataac agggcagcct gagacaaact ttgtgctcga caaagagggc ggagacatat
      841 tctacgagct tacaagtgct aatgtcggca agactatggc cgttgtgctg gatgaccgcg
      901 taaaatccta tgctcgcatc caggaaccaa ttagagaatc ggtacgaata acgggattca
      961 gtgccgagga agccgaagca ctggcgctat tgctgagaac ggccgccctg cccatcagcc
     1021 tttctgtggt aagccagcaa tctatcggcg cttctcttgg agaagacgcc attagtcagg
     1081 gacaaaacgc cataattgtg ggtattttat cgatcttcgt tttcatgttt atttattata
     1141 aatgggctgg aattaatgcg acgatagcac agatactcaa tgtatacctc atgctctcgg
     1201 tcttgacagc cttcaagctg actctcaccc ttccttcgat agcgggtttt gtgctcacta
     1261 ctggaatggc agtagatgcc aatgtcatca tcttcgaacg catcaaggaa gagatgaaag
     1321 caggcaagag tagagcggcg gctatacatg caggtttcga caaagccttc tgggcagtca
     1381 tggactcgaa cataacgacg ataattgcgg cacttttctt ggctcagctt ggaaccggcc
     1441 caattcaggg ttttgcggtt tccttggcaa taggtaacat aacgagtctt tttacctccc
     1501 ttttcgtgtc gaggctgatt tttgatttcg aaactgatgt gctaaagctc aagacagtgt
     1561 ccataagctg gaggatccga taatgaaaag cataattaaa ttcagcaaat tctttttgcc
     1621 cgcgataatt ataagtctat ccattatagc ttttgggctt gccggattct tcactaaagg
     1681 cttgaacctg ggcgtggact ttcaggcagg aataaatgag acgattcagc ttgcctatcc
     1741 agccggacaa gtcgttttcg aaggtaaggg taatgctgaa ctgactatcg gagagagcca
     1801 gctaacgctt gtcttctcag gcgcagaagc gcagaagcgt actgtgctgt tcgattacaa
     1861 gacatactcg acccttgccg acataaaagc tgcactggaa aaagagcagg gaatcagagt
     1921 tgaccttgca gaaggaagcg attctcttcc ttctaatctt cttgtgccta cttaccaggg
     1981 caattctctg ttgagcctaa atcctgtaaa actctaccgc gcgccaaaag atgatagtga
     2041 gctctatgca tcgatcgata tcgttcgcga tgctctcagt ccactgggag atataacagt
     2101 gcagaccata aacccgccat cttctcagag attccttatc agagttcgag ataacggcga
     2161 agacccccag tttatgacaa atgttcctca gactatacgc aatctcatcg aagataaggt
     2221 cggagccgat aggctagttg tggtaaaagc agattatgtc ggacctcagt attccgaaac
     2281 gcttagcaga caatcgatct ggctggtatt gatgaccctc gtccttattc ttgtctattc
     2341 gacaattcga ttcaaaatag aatacgcaat cgctgctgta gcggcgatag ctcacgatgc
     2401 ccttgtgatg attgccttta tagtatggac aaggatggaa ttcaacacca caactatagc
     2461 agcgattttg acaatattgg gctattccat aaacgacacc attgtgcagt tcgacagagt
     2521 gcgagaggaa cgcaagcttc atccttcgga gccatttgtc gatgtgctcg acagggcttt
     2581 gacgctgact ttaggacgct ccattataac gacgcttacc accatgctgg cagtgctcgc
     2641 tctctacttc tttacaagtg gaagtataaa ggattttgcg cttgcgcttc ttgttggcat
     2701 ggttagcggt gtatactcga caatttttat agccagcgct attgttcttt tcttggaaaa
     2761 caaaaaaaca aagcgacgag ctaaaaccat caaagaaaag actgctccag caggcgcttt
     2821 atctaaacaa taggtgtaca cgatgggttt atgcaaagat agttgagcta cattactcag
     2881 aattgttgac aaaagcgggg aactcaagat actatgtgcc ccgcattcgg cgccgtaccc
     2941 aagtggtaag ggagaggtct gcaaaacctt tatacatcgg ttcgattccg atcggcgcct
     3001 aattgagctg tgctgaaaat gccaggagat tttgtattgc cctcgaataa gattcaagac
     3061 ttttgccgat tttttgataa aagaatgctt ctaaggcgcc agcagatgcg aaggtcacgg
     3121 taacctatca gaaaggctca aattttagcg ctcctttccc caatggcatt tatgaagatg
     3181 cattgatcgt tttggttgtc gctatgtgaa gattattaga tgctcctaat gaaagggctg
     3241 tgtacaagct gacgggcagc tattcaacgc agtattggct ctgtttagaa tgaatgttcg
     3301 gtaggtgaat gtggtgattg cgattttgct ggggattaaa gaaaatggag aaaataaaag
     3361 ttttatagcc tattgacatt tttaagaaat gagagtaaat taaatgcaat tttattatca
     3421 taatcgtcta tggaggttga ttatgaaaaa
//
LOCUS       DAOOWY010000157         3442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_27525_length_3442_cov_22.351934, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000157 DAOOWY010000000
VERSION     DAOOWY010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27525_length_3442_cov_22.351934"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1427
                     /locus_tag="PK105_08500"
     CDS             174..1427
                     /locus_tag="PK105_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOU61795.1"
                     /translation="MQKKMKVIALIVVGLFVVSFVAAGPKEDIAATVDRISPDLIAMS
                     DFIHDNPELGNKEYKAVELLTGYLVQNGFKIEKGVAGLETAFIATYENAGGNGPAISF
                     LAEYDALAEVGHACGHNIIGTSCVGAAVALAKNLGSIPARVIVFGCPAEETTSGKLPM
                     AAEGLFKRADIGMQMHPGSGEPTIGSKSLALNLVDFIYEGKASHAAASPELGRSALDG
                     VMLMFMGTEFLREHVKSDIRIHGIVTNGGAAANVVPEKAAARFYVRGLERPYLNTVVE
                     RVYNIARGAAMMSETKLTITEIKQYDNVVNVPSFMDIADEAMKEFGIPNAKKVGPCDP
                     AGGSTDFGTASSQIPALIFGLPVAPADVAGHSREYAIATKSPQGNEALVNASKIMAYL
                     GYRFATEPELLDQVRADWEKVVSGN"
     gene            1755..2357
                     /locus_tag="PK105_08505"
     CDS             1755..2357
                     /locus_tag="PK105_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61796.1"
                     /translation="MNEEPSISPDQQLELIARMLEESRKTFLSTGFSSMVWGGFVSIG
                     TALSYAFESWQWYAAIGLLWALLMLSAFLIVLQHSRHAQHKHLKTTSLHKISNEMWTF
                     VVGVTVLYGIAGYFRPQNISLSYVLCVISLLVGTAYWISSSMTHYLLLRIVSALWIVC
                     SIVVLLVQAFWSPAVVGICAFLCEFVPGIYLYARERKESV"
     gene            2329..2682
                     /locus_tag="PK105_08510"
     CDS             2329..2682
                     /locus_tag="PK105_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOU61797.1"
                     /translation="MQENEKNQYSYQSFDEMLSSRIRLAALALLAGCDEAEFTYIRDV
                     IQTTDGNLASHLRKLEESGYVQYQKRFIDRKPTTYYRITPKGRSALLDYSRQLAELLK
                     NSNNIGGSSNEKENK"
     gene            2663..3268
                     /locus_tag="PK105_08515"
     CDS             2663..3268
                     /locus_tag="PK105_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61798.1"
                     /translation="MKKKTNKILCTTLGQILAVFVFLVSAIMLPAKASSQASQIEKAP
                     VSASHISASRMSLSFSLQEWGNEFGTGFQITSPWFWHEKTAVRLGGSALWKQDANWAT
                     FYALRLGLVGATFMRNEDIRLYGEGGFLFLFPAASFDSDPFAFGGYGHFGFEFFMPKA
                     IASLSSSYFIELGTNGVGAKTDKGQLYLNGFSVSTGLRVYL"
BASE COUNT          889 a          763 c          844 g          946 t
ORIGIN      
        1 tgtccattac gatggacaaa aatctttcga cgggattgac tggattttcg ggatttccat
       61 gatagatagc ggatattttg ctttcctatg tttacagctc gcccatcttg tgaaatagtc
      121 ttttatgcag cagtactaca aaagaataca gaatgctgca aaggaggatc atcatgcaaa
      181 agaaaatgaa ggtcatagcc ctcattgttg tcggtctctt tgtggtttca ttcgttgcag
      241 cagggccaaa agaggatatc gctgctacgg tggatcgaat ttcgcctgat cttatcgcga
      301 tgtccgattt catccatgac aatccggagc tgggcaacaa agaatacaag gctgtagagc
      361 tgcttacggg ctatcttgtg caaaatggct ttaagattga aaaaggtgta gccggccttg
      421 agactgcttt tatcgcgacc tatgaaaacg caggcggaaa cggcccggca atctcctttc
      481 tagctgaata tgatgcgctt gcagaagtag gccatgcctg tggacacaat atcattggca
      541 cttcctgcgt gggagccgct gtcgcccttg cgaagaatct tggatcaatt cccgccagag
      601 tgattgtatt tggatgccct gcagaggaaa caacaagcgg aaagctgccc atggctgctg
      661 aaggcctctt taagcgtgca gatatcggaa tgcaaatgca tcctggctca ggagaaccaa
      721 cgattggatc gaagagcctt gccctcaatc ttgtggattt catctatgaa ggaaaagcaa
      781 gtcacgcagc tgctagccca gaactaggac gtagcgcgct cgatggagtc atgctgatgt
      841 tcatgggtac ggaattcttg cgcgagcacg ttaaatccga tatccgcatc catggcattg
      901 tgaccaatgg cggcgctgca gccaatgtcg tgccagaaaa agctgcagca cgcttctatg
      961 tgcgtggcct tgagaggcca tacctcaata ccgtggtaga gcgggtctac aatattgcgc
     1021 gtggcgctgc gatgatgagc gaaacaaaac tcaccatcac ggagatcaaa caatatgaca
     1081 acgttgtgaa tgtgcctagc tttatggata tcgccgacga agccatgaag gagtttggca
     1141 ttccgaacgc aaagaaggtt ggaccttgcg atcctgcggg cggttcgacc gatttcggca
     1201 cagcatcctc gcaaattccc gcgttgatct ttgggcttcc tgttgctcct gccgatgttg
     1261 caggacattc tagagaatac gccatagcta ccaaaagccc acaaggtaat gaagccctag
     1321 tcaatgcttc caaaattatg gcctaccttg gataccgatt tgcgacggag ccggagcttc
     1381 tagatcaggt ccgcgccgat tgggagaagg tagtctctgg aaactgaggt ttaagcaaac
     1441 atttatcgaa gagaattgca attggataaa gtaacagcta ggctgagctt aggctaattg
     1501 gctccataag ccggcttgtc tcctctggca agagaggatg agccggcttt tttaagtttc
     1561 ttcttaagag ttatttttaa gagttatttg agagtctatt tttctatgct aaagagtccc
     1621 ttaatcatgc aaaattctaa tctcctcaga tcttttaaaa attcataatt tagactttac
     1681 atttcaaagt actttgcgct ataattcata tataaagaaa tactaggagc accgactccc
     1741 aaggagcgtg aatcatgaat gaagaacctt ccataagccc ggatcagcaa ctggagctca
     1801 ttgcgcgcat gctggaagaa tctcgcaaga cctttctatc aacggggttc tcttcgatgg
     1861 tatggggagg ctttgtcagc ataggtaccg ccttgagcta tgcctttgag tcatggcaat
     1921 ggtatgcggc tatagggcta ttatgggctc tccttatgtt gagtgccttt ctgatagtgc
     1981 ttcagcatag caggcatgcg cagcataagc atttgaaaac gacatcgctg cacaaaatct
     2041 ccaatgaaat gtggaccttt gtagttgggg tgacagtact ctatggaata gccggatatt
     2101 ttcgcccaca gaatatttct ctttcctacg tcctttgtgt aatttcgctc cttgtaggca
     2161 ccgcatactg gataagcagc agcatgacgc attatctgct cttgcgaata gtgagcgccc
     2221 tttggattgt ttgcagtatt gtcgttctgt tggtacaggc cttctggtcg ccagcggttg
     2281 tgggaatatg cgccttctta tgcgaattcg taccagggat ctatctctat gcaagagaac
     2341 gaaaagaatc agtatagcta tcaaagcttc gatgaaatgc tctcatctcg aatacggctt
     2401 gccgcgcttg cgttgctggc cggctgcgat gaagcagagt ttacctatat tcgcgatgtg
     2461 atccaaacga cagatggcaa tctagcaagt catttgcgaa agctggaaga aagcggatat
     2521 gtgcagtatc aaaagcgatt tatcgaccgt aaacccacta catactatcg aattactcca
     2581 aaagggaggt ccgccctttt agactatagc cgtcaattgg cagaattatt gaagaattcc
     2641 aataatatag gaggttcatc aaatgaaaaa gaaaacaaat aaaatactgt gcacaacatt
     2701 gggtcagatt ttggctgtat ttgtattcct tgtttctgca attatgctgc cggccaaggc
     2761 atcttctcaa gcttctcaaa ttgagaaagc acccgtcagc gcaagccata tttccgcgag
     2821 ccgtatgtcg ttaagctttt ctcttcagga atggggcaac gaatttggca caggctttca
     2881 gattacaagc ccctggtttt ggcatgaaaa aactgcggtc agacttggtg gctctgcctt
     2941 gtggaagcaa gatgccaatt gggcgacctt ctatgcgctt cgattggggc ttgtgggggc
     3001 gactttcatg agaaatgagg atattcgact ctatggagaa ggcggattct tgtttctctt
     3061 tcctgccgca tccttcgata gcgatccttt tgcttttggc ggctatggac attttggctt
     3121 tgagtttttc atgccaaagg ccatcgcttc tctttcgtct tcttatttta tcgagcttgg
     3181 tacgaatggc gtcggagcaa agaccgataa aggccagtta tatcttaacg gcttttcggt
     3241 gagcaccggt ttgagggtat atctataaag attatttagg gccgatttcc ataatccgag
     3301 cgcgattacc ttgaatcttg catcagaagc cattgcttgg cgaattcgag ttcgacatcg
     3361 atgcccaagg catacgcaat gatgttttgc caagttcgtg ggatgcgaat ctcgggctgc
     3421 actccgcctt tgccagaagc at
//
LOCUS       DAOOWY010000158         3337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_28638_length_3337_cov_25.705972, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000158 DAOOWY010000000
VERSION     DAOOWY010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28638_length_3337_cov_25.705972"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1360)
                     /locus_tag="PK105_08520"
     CDS             complement(113..1360)
                     /locus_tag="PK105_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU61799.1"
                     /translation="MLYLQLAQNHQRDYHGAMIPRVATQLIDRLASQFPAVVITGPRQ
                     SGKTTLVRSRFAEKPYLNLELPDMRQRIIDDPRGTLYPLRAKGAVIDEAQRFPELNSW
                     LQGIIDEDPRAGLWILTGSNQPLLRQSATQSLVGRAAFAKLLPFFTDELAATSLFANA
                     STDECLYRGWYPPLFDRPFIPSDWYAQYVALYLERDLSQLINVKDMREFEKFLALCAG
                     RTGQLLNLSELARDVGISHTTARGWLSILEQSFLVFELAPYSKNFQKRIIKSPKLYFY
                     DCGLAAWLMGIREPRQITTHPLRGALFETMVVADIIKRASAHGSDARFSFWNAPSVGE
                     IDLIIEEGAAITAIEIERGTTFHPEMANTLIKWATIAEPIAQRILIYDGDEKFQFKGI
                     EVVPWRIVGRDEQDNVLTGLRQD"
     gene            2343..>3337
                     /locus_tag="PK105_08525"
     CDS             2343..>3337
                     /locus_tag="PK105_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61800.1"
                     /translation="MKIALISCTKLQRQYECPARELYSESAWFREAYWYAKLVVDKIF
                     ILSSEYGLVPEGMVIIPYDNDLGDIPAAERREWAERVLNELRKVSDLEHDEFIVLAGK
                     SYYENLLPHLVHVELPLGHMRQGEQRAELRRLIKPEMESNNALALHMLFNDLPRFDWK
                     MIDQIPYRNGIYVMFEKGESYHSMDRIVRIGTHRGQDRLLERLRDHFVREDADSSILR
                     KNIGRAFLNMGLNPYLQVWEIDMHYSENIRNFGHLINKELDTKLEARISEYLRKNITF
                     ICIPVEEEAERRRLEEGLIASLNMDPSFGPSSNWLGLNSPMPEIARSGLWNSQHL"
BASE COUNT          916 a          722 c          786 g          913 t
ORIGIN      
        1 gagcaaaacg aatagagccg tttatattta tgcggatgga agtagaggtt gtttggtgca
       61 agcctggctc tgccttcggc ttttttatct tgccctcaaa ccaaaaatcc agtcaatctt
      121 gtcttaatcc cgtcaataca ttatcctgtt catcccgtcc aactatccgc cacggcacta
      181 cttctatccc cttaaactga aacttctcgt cgccatcgta tatcagaatg cgttgagcga
      241 taggttcagc aatcgttgcc cactttatga gcgtgttcgc catttcgggg tggaaggttg
      301 taccgcgctc tatttcgatc gcggtgatcg ctgcgccctc ttcaatgatg aggtcgatct
      361 ctcccacgct gggcgcattc caaaaagaaa agcgtgcatc actgccgtgt gctgaagctc
      421 gctttatgat atcggcaacc accattgtct cgaagagagc cccacgcaaa ggatgggtcg
      481 tgatctggcg tggttctcgg atgcccatga gccatgccgc aagtccacag tcataaaagt
      541 agagctttgg gctttttatg atgcgctttt gaaaattttt agagtacggc gcgagctcaa
      601 acacaaggaa gctttgctcg aggattgaaa gccatccgcg cgcggtggta tgagaaatgc
      661 ccacatctcg cgcaagctcc gagagattga gcaattggcc tgttcgacct gcacagaggg
      721 ccagaaactt ttcaaactcc cgcatatcct taacattgat gagctgtgag aggtctctct
      781 cgaggtagag cgccacatac tgcgcatacc aatcagaagg gataaaaggg cgatcgaaaa
      841 gaggcggata ccatccgcga tagaggcatt catcagtact tgcattcgca aaaagtgaag
      901 tagcggcaag ctcatcagta aaaaaaggca aaagcttcgc aaatgcagcc cttcctacga
      961 gcgactgggt agccgactgt cgcaaaaggg gttgattgct tcctgtaaga atccataatc
     1021 cggctcgagg atcttcgtcg attatccctt gaagccatga attgagttca ggaaagcgct
     1081 gcgcttcatc gatcacggcg cctttggcgc gaagtggata gagtgtgcct cgcggatcat
     1141 cgataatccg ctggcgcata tcaggaagtt cgaggttgag atacggtttt tcagcaaagc
     1201 gcgatcgcac gagcgtcgtt ttgcccgatt gtcgtggccc cgtgatcacc acggcgggga
     1261 attgtgacgc gagcctatcg ataagctgcg ttgctactcg agggatcata gctccatgat
     1321 agtctctttg gtgattttgt gcaagttgca agtacaacat gcaatgtgca tcctgtcatt
     1381 tggtccaaaa aatcttcaat aactatacat atgtatagcc aatctctaca ttttttctaa
     1441 aattagcact atcaatcttg atttcctttt gaagctatac tacgctgtgg aatgttcaca
     1501 aaatgaacat tcttttgggc aagagttacg ctgaaccccg catcttgcat cctgcatccg
     1561 tgcgcgcttg tcctacaaaa cccttgtaat gcttgccagc gtgtttcggc cgcggcaagg
     1621 tgagagggcg tttttcctct tttttaagct tccttgggcg agtgggcttt tgagcccacc
     1681 aagaggaaga gacttcgtgt gctcctattt gtctagtcta tttgcatttg gtcaggctaa
     1741 acgcaatcag atagtgcatt tcgtcaaact tggaattcgt ttctcaaatt tgcggaactt
     1801 ctttttgctc agagcgatgg gcgattcaat gcgcttgcat atttgcgcca gcgagtataa
     1861 tagtaagtac ctttctggag cgaatataca aagttcgatg cccgatattg tcgacatctt
     1921 gtggtttgaa aaaagatacc agaccgcttt ccggcacaaa agcaattttt cttgccggtc
     1981 gctgtttctc tcatagacaa tgtagcgatt gttttgagcc tctttattgc ataaatgcct
     2041 tgtagagatt atcaagacag tggcctgtgc taatcctatc gcgctgattt tttacttttc
     2101 cctgtcctat tttcagaata actctgcaac tactaagcga agtagcaaag agagcgtagg
     2161 caataaaaag aaaagatgct cgatatcttc taacgcataa gatgttatag tagtctccgg
     2221 gagcgagaag acaatcttca acgaaagaat tcgatcaatt atttgcaagg aattactgat
     2281 agcctaacaa tgttttataa ccagggaata aacggattta agtgatatca gaggacggga
     2341 aaatgaaaat tgctttgatc tcatgtacga aactacaaag acaatatgaa tgcccagcaa
     2401 gagagttata ttcagaaagc gcgtggtttc gtgaggccta ttggtatgca aaacttgttg
     2461 tagataaaat atttattctt tcttcagaat atggcctagt gccagagggc atggttatta
     2521 taccttacga caatgactta ggagacatac ctgctgcaga acggcgcgaa tgggcagaga
     2581 gggtgctaaa cgaattacga aaagtttctg acctagagca cgatgagttt attgttttag
     2641 ctggtaaatc ttattatgaa aacttgttgc cgcatttggt tcatgttgag ctccccctcg
     2701 gacacatgcg tcaaggagaa cagagggcag agctaaggag actgattaag cctgaaatgg
     2761 aatcaaataa tgccttggct cttcatatgc tcttcaacga tctgccgcga ttcgattgga
     2821 agatgataga tcagattcct tatcgaaatg gtatatacgt catgttcgaa aaaggtgaaa
     2881 gctaccattc catggataga atagttcgca tcggtaccca tcggggtcaa gaccgtctat
     2941 tggaaaggtt aagagaccac tttgtaagag aggatgccga tagtagcatt ctccgcaaga
     3001 acatcggccg cgcttttttg aatatgggtt tgaatccata tttacaagtg tgggaaattg
     3061 atatgcatta ttctgaaaat ataagaaact ttggacactt aataaataaa gagcttgata
     3121 ccaaactcga ggcaagaatt agcgaatact taaggaagaa cataaccttt atctgtattc
     3181 cggtcgagga agaagctgaa cgacgaaggc tagaagaagg tcttattgca tcacttaaca
     3241 tggatccatc atttggcccc agcagcaatt ggcttggtct gaatagccct atgccggaga
     3301 ttgcacgtag cggtctatgg aatagtcagc atttgca
//
LOCUS       DAOOWY010000159         3327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_28741_length_3327_cov_13.286980, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000159 DAOOWY010000000
VERSION     DAOOWY010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28741_length_3327_cov_13.286980"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1218
                     /locus_tag="PK105_08530"
     CDS             211..1218
                     /locus_tag="PK105_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU61801.1"
                     /translation="MRVLFIGGTGNISSACTNEALRKGFEVFHLNRGNRPEKERREVK
                     VLRADIRQPEAVKRAIGSLCFDSVVQFLGFEPKHIEADIQLFKDITHQYIFISSCSAY
                     KKPSLTPIITEETPLENPFWEYARLKAACEKVLMESGHTHSFPYTIVRPSHTYDNGWI
                     PGCFGSAGYGLAWRMLNGKEIIVPGDGQSLWTLTHASDFAVGLVGLLGNPAALSEAFH
                     ITSDEHLTWDAIHTIIAEELGVSPRIVHVPSDYIARINPERGAGLLGDKAVSVLFDNS
                     KIRRFVPEFRPQITFREGIRRSLAWYDANPQLKMPDSIMNKEMDTILGYWNKGLSVLK
                     M"
     gene            complement(1279..2505)
                     /locus_tag="PK105_08535"
     CDS             complement(1279..2505)
                     /locus_tag="PK105_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU61802.1"
                     /translation="MNIIPRPRYIDRIRPYIGTKLIKAIIGQRRVGKSYILLQLAEYL
                     RNEHPDWFILHIDLEQLEWRHLRTGEDLVREVMSRIPYDAQIGRQVSIALLLDEVQQL
                     KNFEIAVRGFAADDRFDVYISGSNADLLSSDIATLFAGRAIRIEVFSLTYDEFLTFHR
                     LEDNDQALNKFLYCGGLPFLANLPYDDVVFREYLSAVLDSVVLKDVVDRYGIRSPSLL
                     ERILEFIADNIGNPTSARNIANYLRSIRIDASPQSVLDYLGYLTRSFALLRCPIENVG
                     GKKVLEGIAKYYFQDLGLRSVVHNSTSSDISKIIENAVFIRLLADGWKVSTGRIGERE
                     VDFIAEKGQGRCYIQATYLMPDKATKQREFGALAEIEGGWPRFVISMDPIVHDWQGIH
                     HIRLRDFLRDGLLANR"
     gene            2638..3039
                     /locus_tag="PK105_08540"
     CDS             2638..3039
                     /locus_tag="PK105_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61803.1"
                     /translation="MLAYVYSSLIITEIKQYDNVVNVPSFMDIADEAMKEFGIPNAKK
                     VGPWDPAGASTDFGTASSQIPALIFGLPVAPADVAGHSREYAIATKSPEGNEALINAS
                     KIMAYLGYRLAAEPELLAQVRADWEKVVSGN"
BASE COUNT          963 a          754 c          732 g          878 t
ORIGIN      
        1 agcttttctt gagatataca gcatttagct cgagttcctg tagcaattta cgaacacgga
       61 gttttttctc ggcgatttct tgatggacaa attcgaacat caattctctc ctatagtgaa
      121 tttccttgac aaaacaaaag agttcctata aatattacgt tgtaagacgt cagttgtcaa
      181 taaaattttt tatcaaaatg aggaaatgag atgcgtgtac tattcatagg gggaaccggc
      241 aatatttcat cggcttgtac aaacgaagcg ctcagaaagg gcttcgaggt ctttcatctt
      301 aacagaggaa acagaccgga aaaggaaaga cgagaagtaa aagtcttgag agcggacatc
      361 aggcagccag aggccgtaaa aagagccatt ggatcgctgt gcttcgatag cgtcgttcag
      421 ttcctgggat tcgagcccaa acatatcgaa gccgatatac agctttttaa ggatatcacc
      481 catcaatata tattcataag cagctgttca gcgtataaaa aaccctccct aaccccaatt
      541 atcaccgaag aaacacctct cgaaaacccg ttttgggaat atgcacgtct aaaagcagcc
      601 tgcgaaaagg tcttaatgga aagcggacac acgcattcct ttccgtatac gatcgtgcgg
      661 ccttcgcata cttacgacaa tggctggata ccagggtgtt ttggctccgc gggttatggc
      721 cttgcgtgga ggatgctcaa tggcaaagaa ataatagtgc cgggagatgg tcagtctcta
      781 tggactctca cacatgcatc ggactttgcg gtgggccttg ttgggctctt aggaaaccct
      841 gccgcgcttt cggaagcctt tcatattact tcagatgagc atctcacctg ggatgcaata
      901 cacacgatta ttgcagaaga gcttggagtc agcccgagaa tcgttcatgt cccttcagat
      961 tacattgcca gaataaatcc cgagcgcgga gcggggctcc ttggcgacaa ggctgtatca
     1021 gtgctcttcg ataattccaa gatcagacga ttcgtccctg aattccgccc gcagattacc
     1081 tttagagagg gcattcggcg ctcgctagcc tggtacgacg ctaatccaca gttgaaaatg
     1141 ccagattcca taatgaacaa ggagatggac acaattttgg gatattggaa taaagggctc
     1201 tcggtgctaa agatgtaaaa actccaaact gatctgattt ctgggactct cacggtcatc
     1261 gagcatgtaa gagtatattt atcgatttgc caagagtcca tcgcgtagaa aatctctgag
     1321 cctaatatga tgaatcccct gccaatcgtg gacgatagga tccatcgaaa ttacaaagcg
     1381 aggccagccg ccttcaatct ctgcaagcgc tccaaactct ctttgtttag ttgctttgtc
     1441 tggcatgaga tacgtagcct gaatatagca gcgcccttgt cccttttcgg caataaaatc
     1501 gacctcgcgc tcgcctattc gacccgttga gactttccag ccatccgcaa ggagccttat
     1561 aaatacagca ttttcaatga tcttgctgat atctgagctt gtgctgttat ggacgactga
     1621 tcgcaatcct agatcttgaa aataatactt agcgattcct tcaagtacct ttttgccgcc
     1681 cacattttca atagggcatc gcaggagcgc aaacgatcga gtaagatatc ccaagtaatc
     1741 gagcaccgat tgtggcgacg catcaatccg tatacttctt aggtaattgg caatattcct
     1801 cgccgaggtg gggtttccaa tattgtctgc aataaattca agaatccttt ctaggagcga
     1861 cggacttctt ataccataac ggtcaaccac atctttcaat actacagaat cgagcactgc
     1921 gctcaaatat tcgcgaaaca cgacatcgtc atacggcaga tttgcaagaa atggcaagcc
     1981 accacagtac aaaaatttat tgagtgcctg atcattgtct tcaagacgat gaaaagtcaa
     2041 aaactcatca taggtaaggg agaacacctc gatgcggata gcccttcctg caaacaatgt
     2101 tgcaatatcg cttgaaagca gatcagcatt cgatccagaa atatacacat caaaacgatc
     2161 atccgcagca aatccgcgta cagcaatttc aaaattcttc aattgctgta cctcatcaag
     2221 aagaagcgcg atcgaaactt gtcggccaat ttgagcatca taggggatcc tcgacatgac
     2281 ttcgcggaca aggtcttcac ctgttctgag atgccgccat tcgagttgtt ccaaatcgat
     2341 atgcagtatg aaccaatcag gatgctcatt gcgaagatat tctgcaagct gaagaaggat
     2401 ataacttttc cctacacgcc gctgaccaat gatcgccttg ataagttttg taccgatgta
     2461 agggcgaatt ctgtcaatat accttgggcg aggaatgatg ttcattaggg tgcgcctcca
     2521 gtgtccatta tgatggacaa aaccataatt tgcaagctat tgtccattat gatggacaaa
     2581 aatcattcga cggaatttat tggattttcg ggatttccat gatagatagc ggatattttg
     2641 cttgcctatg tttacagctc gctcatcatc acggaaatca aacaatacga taatgtagtg
     2701 aacgtgccaa gctttatgga tatcgccgac gaagccatga aggagtttgg cattccgaac
     2761 gcaaagaaag ttggaccttg ggatccagct ggcgcatcga ccgatttcgg taccgcctca
     2821 tcgcaaattc ccgcattgat ctttgggcta cccgtggctc cagcagatgt agcgggccac
     2881 tcaagggaat acgccatcgc aacaaagagc ccggaaggca atgaagccct tataaacgcc
     2941 tcgaagatca tggcctacct tggttatcgc cttgccgccg aaccagagct tttggctcag
     3001 gttcgcgctg actgggagaa ggtagtttct ggaaattgag gttagagtaa acagctatcg
     3061 aagagaattg cgattagata aaataacaga taggctgagg ttaggctaat tggctccata
     3121 agccggcttg tctcctctgg caagagagga taagccggct tttttaagtt tcttcttaag
     3181 agttatttga gagtctattt ttctatgcga aagagtctct taatcatgca aaattcaaat
     3241 atcctcaggt cttttaaaaa ttcataatta atactttaca tttcaaagta ctttgcgcta
     3301 taattcatat ataaagaaat actagga
//
LOCUS       DAOOWY010000160         3321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_28801_length_3321_cov_31.170851, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000160 DAOOWY010000000
VERSION     DAOOWY010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28801_length_3321_cov_31.170851"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            542..1768
                     /locus_tag="PK105_08545"
     CDS             542..1768
                     /locus_tag="PK105_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HOU61804.1"
                     /translation="MMYLDIWQNKENLSINQNETDQKGKIGKATLDNSSIDTIRLQAP
                     IPPSSPLPMKTQLSDADAAWNSFSAQRMRQVREVIRLKHRSLRTEKAYLAWTHRFLDF
                     VWSKKLVREAEDKKGDYIITADHLRAFLSYLALEVHVSAATQEQALNALLVLFRTILH
                     VDIEGLSSVLRAKKRKRLPVVLSCNEVAALLTALRQPYRLMASLMYGCGLRLEECLSL
                     RVKDIDFDHDTIEVRSGKGGKDRLTVLPGVLKGDLRAYLAELRQAWEQARRRDLPGVF
                     LPEALARKYPSLSKEWSWYWLFPARSPCANTRTGEIGYWHMHPSVIQKKIKEGVLRAG
                     ITKMASAHTLRHSFATHLLEDGYDIRTIQELLGHSNVQTTMIYTHVAMKNKRGVCSPL
                     EHLQDITFRKQSSNAE"
     gene            2182..3084
                     /locus_tag="PK105_08550"
     CDS             2182..3084
                     /locus_tag="PK105_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61805.1"
                     /translation="MLGDIRPPIVRDAKLKNASEFEIEFDEPVCVCREAFSTEPKRIA
                     ACAESSGEKVSITLLPQPVPGEKVILVGTVEDVYGNSTHVQVQFKGYNDRPARIILTE
                     VQAAKNTSKKSPHRDYIEFTVQKGGNLGGMFVQWASSTKIMKYDFIPCEVKAEEIIVL
                     HLAPEGILEEKDETGEDTALSGGIDATAKGRDFWSEAGGLPDSSGAISVYEREGSLPI
                     DGFFYGEGSKSGSLESSKLISIVQELSDAGVWSSDSPPKWENAFLWKPSASRPFIRTS
                     ISESGVSAWKVGESGSQTPGSFSR"
     gene            complement(3077..>3321)
                     /gene="trxA"
                     /locus_tag="PK105_08555"
     CDS             complement(3077..>3321)
                     /gene="trxA"
                     /locus_tag="PK105_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU61806.1"
                     /translation="WAEWCMPCKMIGPSISQIAETYKDKIKVGKLNVDNEAEIASQYG
                     IISIPTLIIFKDGQAIRQKVGAMPRHEIEKLFSDLI"
BASE COUNT          908 a          645 c          831 g          937 t
ORIGIN      
        1 ctaacatatt ctacctcaga aaaccctata agaaaacaat caagaaattc agataaacaa
       61 aagcgcgata agcttcgtgc aaaaattgtt gtgctgtttt gaagcgcgaa taagtttctc
      121 tgcgtcatat ttgatagaag cgtgataagt agtagagcaa gagctcttct ttcgcgatta
      181 cgtttcttac ctgcttcgtt tcaatcgcta tttgctattg gctcagagag cgcttcttta
      241 acagagcaat tagccaaggc acatatccgg tcagcttcat gacacagttg cataaaggaa
      301 aaggaggctt cctgtgtcgt cacactataa taaaagcggt tttatgcagt tcttgattga
      361 aaagtgccat tttggagaca aaaaaactaa atgggcctgc tattgggttg aaaggtttga
      421 gcaattattc cccgagtggg agcaagaccg cgagcgtgct atcgaagcat ttagtgatct
      481 cttatgcaag aagaaatctg atatagtcgt gaattgggca ctccaatcgg tgagagtgtt
      541 tatgatgtac cttgatatat ggcaaaataa ggaaaatctg agcataaacc aaaatgaaac
      601 tgaccaaaag ggcaaaatag gtaaagcaac gctggacaat tcttctattg atacgattag
      661 gcttcaggcg cctattccgc ctagttctcc gttacctatg aagactcagc tttcggacgc
      721 ggatgcagca tggaattctt tctcagcaca gcgtatgaga caagttcgag aagttattcg
      781 gctcaaacat cgctcactgc gcaccgaaaa ggcatatctt gcatggaccc atcgatttct
      841 cgattttgtg tggagcaaga agcttgtaag agaggcagaa gataagaaag gcgactatat
      901 tatcaccgca gatcatctgc gtgcgttttt aagttatctt gcgctggagg tacatgtgag
      961 tgcggcgaca caagagcagg cgcttaatgc gcttcttgtg ctgtttcgta cgattctgca
     1021 tgtcgatatc gaggggcttt cgagcgtatt gagggcgaaa aaacgcaaaa gactacctgt
     1081 agtacttagc tgcaatgagg tggcagcatt gctcacagcg ctgcgtcagc catatcggct
     1141 tatggctagc cttatgtatg ggtgtggttt gaggctcgag gaatgtcttt ccctgagggt
     1201 aaaggatatc gattttgatc atgatacgat cgaagttcga tcgggaaaag gagggaagga
     1261 taggctcacg gtccttccag gggtgctcaa gggcgatttg cgggcatacc ttgcggaatt
     1321 gcggcaagct tgggagcagg ctagaaggcg cgatctacca ggggtgtttc ttcctgaggc
     1381 gcttgcacga aaatatcctt cgctttcgaa ggaatggagt tggtattggc tctttcctgc
     1441 acgaagtcct tgcgcaaata ctcgtaccgg cgaaattggc tattggcaca tgcatccttc
     1501 ggtgattcag aaaaaaatca aagagggggt tttgcgggcg ggtatcacga agatggcttc
     1561 ggcacatacc ttgcggcata gttttgcgac gcatctattg gaggatggct atgatatccg
     1621 cacgattcag gaacttctag ggcattcaaa tgtccagaca acgatgatct atactcatgt
     1681 ggcgatgaag aataaacgcg gcgtgtgcag tcctttagaa catctgcaag atattacctt
     1741 taggaagcaa agctccaacg ctgagtaagg cttttaccgc gatgcaaggt gcgaccgccg
     1801 caacaggttc acatcttagt atatcgcgct tttgtaaggt tttaccttta aagctattgt
     1861 agatttgcct tttggcacag tcaattgtct ttcatttaaa tattgatttt ttctagatat
     1921 gatttttgaa gcaatgtatt ggattgcttg cgaaatatca tagaacgcat cgatttgagc
     1981 tcctagggaa tgacgctaga aaaaggtgct cttttatata tatggagacg aaggaatatg
     2041 tcgcgaataa gaaataaaag gaatagccca catctaagat ggagggcaaa atcctataca
     2101 tctcaattct ggtgcttttt caatttattc ttgatcatgt tcttgcttac agcttgttcg
     2161 cctccatcgc tggagaataa gatgctcggc gatattcgtc ctcctatagt gcgagatgct
     2221 aagctaaaaa atgctagcga atttgaaata gaattcgatg agcctgtatg tgtgtgcaga
     2281 gaggcttttt ccactgagcc taaacgaatt gcggcatgcg cagagagttc aggggaaaag
     2341 gtgagtatta cccttcttcc tcagcctgtt ccaggagaaa aagtgattct tgtgggcact
     2401 gttgaagatg tgtatggaaa tagcactcat gttcaggttc aattcaaggg ctataatgat
     2461 aggcctgcac gaattattct aactgaggta caggctgcta agaatacctc taaaaagtct
     2521 cctcatcgcg attatattga atttactgtc cagaagggtg ggaatctagg cgggatgttc
     2581 gtacaatggg caagttctac aaaaattatg aagtatgact tcataccttg cgaggtaaag
     2641 gcagaggaga ttattgtcct tcatctagcg ccagaaggta ttctagagga aaaggacgaa
     2701 acgggagaag atacagcact ttctggcggc atcgatgcta ctgccaaggg aagagatttt
     2761 tggtcggaag cgggtggttt gcccgattcg agcggagcta tctctgtcta tgagcgagag
     2821 gggagtcttc ccatcgatgg atttttctat ggcgaaggct ccaaaagcgg ttctttggag
     2881 tcttcgaagc taatctcgat agtgcaggag ctttcagatg caggagtatg gagttccgat
     2941 tctcctccga aatgggaaaa tgcatttctg tggaaacctt cggcttctcg gccttttatc
     3001 cgaacatcga tatctgaatc tggtgtaagt gcgtggaaag tcggagaaag tggtagtcaa
     3061 acgcccggtt ctttttctag ataaggtcgc taaataactt ttcgatttcg tgtcgaggca
     3121 ttgcgccgac tttttggcgt attgcttggc catctttgaa gattattagt gttggaatag
     3181 aaattatacc atattgagaa gcgatttcag cttcgttatc tacattgagc tttcccacct
     3241 tgattttatc tttataggtc tctgctattt gggaaataga tggtcctatc attttgcagg
     3301 gcatgcacca ttcggcccaa a
//
LOCUS       DAOOWY010000161         3306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_28978_length_3306_cov_26.918794, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000161 DAOOWY010000000
VERSION     DAOOWY010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28978_length_3306_cov_26.918794"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1177
                     /locus_tag="PK105_08560"
     CDS             71..1177
                     /locus_tag="PK105_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOU61807.1"
                     /translation="MFEFVHEEIAEKKYRVRKLLQELELNAVYLKKSSNFSWITGGGY
                     NIVGMATEIGVAGALITADREFIVCNNIEAPRMEKEEHLAEQGYEIISFPWYDDQEAS
                     IVNSLSGGKFGADHAFPGSLDISSKINPLRYSLTQWEIERYRKQALDTARIAEETALT
                     IKPGDEERAVIGRLSERLWREGMDFVVAFCAADERISLFRHPVATDKKIEKRAMISVN
                     SRKRGLIVSITRFVQFGKVPADLRKRYEDNVYVDTAMMTATIPGRPASEVFEAALHAY
                     AEKGYPEEYKLHHQGGAIGYVGRDYRVTAKTREIIQENQAFTWNPSITGTKSEDTMLA
                     TKNGPIVFSEPSLFPVLTMERNGYVFKRPDIMEL"
     gene            1310..2281
                     /locus_tag="PK105_08565"
     CDS             1310..2281
                     /locus_tag="PK105_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOU61808.1"
                     /translation="MKTKKAVLLISALLLLSTFCLGAQQKEKFPTREIEIMVPWAAGG
                     ATDLMVRIFSPVIQKYLGVPILITNKPGGAAVPGYVEAMTKKPDGYYLVAWATPSITV
                     THMQKTPYDANSFEPIINIVTAPVWFLAPASSPYNNLKDLVEDAKKRPGKINLGNAGA
                     GGGTHMIALAFESAVGVKFNHVPYSGGAPAVTAAVAGQVDAITCGPPEGVPQLAAGQL
                     KCLGVFSEKRLEEFPNYPTAKELGYNFAMGQWRGLAAPKGTDPERIKIIHDAFKAAMS
                     DPEVIKLAQQSGLLFDYRNTEDFKKLIQEEDAYYMKVVKENKLGSKY"
     gene            2447..2956
                     /locus_tag="PK105_08570"
     CDS             2447..2956
                     /locus_tag="PK105_08570"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOU61809.1"
                     /translation="MAKADIVLGIIFAALSIVFYIGTLSFPKFSIGINPKVYPLIIII
                     STFALSVTLMVQGILRLRKEKTPPAAPKTSAEKGSSEPSSPELTLPRGKTALKLIVLA
                     IGMLAYVIGFEPLGYIIVTPLLMALTMYLFGERKPFRIIAVSVLVSVILYGVFRTVFR
                     VPLPRSIIW"
     gene            2970..>3306
                     /locus_tag="PK105_08575"
     CDS             2970..>3306
                     /locus_tag="PK105_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOU61810.1"
                     /translation="MTGIINAILYVLANFHFLIIGVAGGIIVGAIPGLSGSTGIILML
                     PFLIYIDPPAALIMMSGLFCGSMFGGSIASILVSTPGTPSAAATVLDGYPLAKKGEAG
                     KAMGVALVAS"
BASE COUNT          885 a          757 c          848 g          816 t
ORIGIN      
        1 acgtcttaca acgtaagatt tataggaact ctttggtttt gtcaagaaaa ttcgcagtgg
       61 gagagtatcg atgttcgaat ttgtccatga agagatcgcc gagaaaaaat atcgcgttcg
      121 taaattgcta caggaactcg agctaaatgc tgtatatctc aagaaaagct ccaatttttc
      181 ttggattacg ggaggcggct acaatattgt tggaatggct accgagatag gagtcgccgg
      241 ggctctcatt accgctgacc gcgaattcat cgtatgtaat aatatcgaag cgccgcggat
      301 ggagaaagag gagcatcttg ccgagcaggg ttatgagatt atttcctttc cctggtatga
      361 cgatcaggaa gcatcaattg tcaattctct atccggtggc aaattcggtg ccgaccatgc
      421 ttttccaggc tccttagaca tttccagcaa gataaatccc cttcgctatt ccctgacaca
      481 atgggaaatc gagcgatatc gtaagcaagc cctcgacacg gcgcggattg cggaggagac
      541 agcccttaca atcaagcccg gagatgaaga gagggcggtt ataggcagac tctcggaaag
      601 gctatggaga gaaggcatgg atttcgttgt ggctttctgc gctgcggacg agcgtatctc
      661 tctctttcgc catcctgtag ccacggataa aaagatcgaa aagcgggcga tgatcagcgt
      721 caattctaga aagcggggac tcatcgtctc gataacgcgt ttcgttcagt ttgggaaggt
      781 gccagcggat ctcagaaagc gctatgagga caatgtctat gtcgataccg caatgatgac
      841 agccacgatc cctggtcgac ctgcttcgga agtttttgaa gcagccctgc acgcatatgc
      901 ggaaaagggc tatcccgaag aatacaagct ccatcatcaa ggaggagcca ttggctatgt
      961 tggaagagac tacagagtga cggccaaaac tagagagatc atccaggaaa atcaagcctt
     1021 tacctggaat ccatcgataa caggtactaa aagcgaggat accatgctcg caacaaaaaa
     1081 tggccctatt gtcttctcgg agccatcgct tttccctgtg ctcacgatgg agagaaatgg
     1141 ctatgtattt aagaggcccg atattatgga actatgagaa caataacaag gacatcccgc
     1201 cctaaagcag ataatgagtg ttataaaaag aggctatttg caccagaata taaaatattg
     1261 cttgaaaaag gggctctttg ccccgacaac agattaaagg aggtttccaa tgaaaacaaa
     1321 gaaagctgtg cttctgattt ctgcgctcct gcttcttagc accttttgtc ttggagcgca
     1381 gcagaaagaa aaatttccca caagggaaat cgaaatcatg gtgccatggg cggcaggtgg
     1441 ggcaacggat ctcatggttc gcattttttc gccagtgatt cagaaatatc ttggcgtccc
     1501 gattctcatc accaacaagc caggaggggc tgcggttcca ggttatgtag aagccatgac
     1561 gaaaaaaccc gatggctatt acctagtagc ctgggcgaca ccgtccatca ccgtcacgca
     1621 tatgcagaaa accccctacg acgcaaacag cttcgagcca ataatcaaca tagtaacagc
     1681 gccggtgtgg ttcttagctc cagcctcttc tccctacaac aacttaaagg atcttgtgga
     1741 ggatgcaaag aagaggcctg gaaagatcaa tctaggaaat gctggggcag gcggaggcac
     1801 tcatatgatc gcgctggcct tcgaatcggc cgttggtgtc aagttcaacc atgtgccata
     1861 ttcgggcgga gcgccggcag taaccgcggc tgtcgcgggt caagtcgatg cgattacctg
     1921 tgggcctcca gagggtgtgc cgcaacttgc tgctggtcag cttaaatgtc tcggagtttt
     1981 ctctgaaaag cggctcgagg aattcccaaa ttaccctacg gctaaggaac tggggtataa
     2041 ttttgccatg ggacaatgga gagggcttgc ggctcccaag ggaaccgatc ccgaaaggat
     2101 caagataatt cacgatgcct ttaaggcggc aatgtccgat ccggaagtca taaagcttgc
     2161 tcagcaatcg ggcttattgt tcgattaccg caacaccgag gatttcaaaa agcttataca
     2221 ggaagaagac gcctattata tgaaggtagt taaggaaaac aaactgggga gcaagtactg
     2281 agaatacgta ggggcagagc agaatacggc cagccatgac attattgcag cgtgtcaaca
     2341 aatcctggca ttatgaggat ttgagctgcc gcgctggccg ggttggccgg ataattctct
     2401 agacaagcat cttgcacgca tggtcaagcg ggaggatttt ataaggatgg ctaaggcaga
     2461 catagtatta ggaataattt ttgcggctct ttctatagta ttctatattg gaactctttc
     2521 ttttccgaaa ttcagcattg gtataaatcc caaggtttat ccgcttataa tcattatctc
     2581 tacctttgct ctttcggtga cgctgatggt tcaaggtatc cttagactta gaaaagaaaa
     2641 gacgcctccc gcagccccaa aaacctccgc agaaaaaggc tcctcggaac cgagttcccc
     2701 tgagcttact cttccgaggg gtaagacggc gctaaaacta atcgtcttgg caattggtat
     2761 gctggcttat gtaattggtt tcgaaccttt aggctatata atcgtgactc cgctcttgat
     2821 ggcccttacg atgtatcttt ttggcgagcg aaagccattc cgaataattg ccgtatctgt
     2881 gctcgtttcg gtcatactgt acggggtttt caggacagtg ttcagggtac ccctgccgcg
     2941 ttctatcatc tggtgaaagg agaactgcaa tgacgggaat aatcaatgcg attctctatg
     3001 tactagcaaa ctttcatttc ctcataattg gcgtggcagg aggtatcatt gtaggggcga
     3061 ttccgggact atccggctcc acgggcatca ttcttatgtt gccattcttg atatacatag
     3121 acccgcccgc ggcgctaatc atgatgagcg gactcttttg cggttcgatg tttggaggtt
     3181 cgatcgcatc tattcttgtt tcgacgccag gaaccccatc ggcggcagcg acagtgctcg
     3241 acggctatcc gcttgccaag aagggagagg cgggaaaggc tatgggtgtt gccttggtcg
     3301 cctcga
//
LOCUS       DAOOWY010000162         3252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_29602_length_3252_cov_56.364091, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000162 DAOOWY010000000
VERSION     DAOOWY010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29602_length_3252_cov_56.364091"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PK105_08580"
     CDS             <1..602
                     /locus_tag="PK105_08580"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU61811.1"
                     /translation="VVPCPEDGELAPLGEYGRNKIEAENLCREACAAGLPVVMLRPPT
                     ITGSGLDEPFILSLLSAIQKGKPVTLLGKGTNRFQFIHVDDVVDACLLAAEHPDAAGE
                     AFNIGGKDVPSIAELVHLVASAVGSPSKVRLIPTTPAKIAIALLRPFGAAPLEPEHVA
                     IAICDYVFDWSKAQRILGWEPRWSVADALIDTYRAIYGG"
     gene            606..>3252
                     /locus_tag="PK105_08585"
     CDS             606..>3252
                     /locus_tag="PK105_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61812.1"
                     /translation="MSLVDNASKALVSGRIPSPLVRGAAHVVVAILSAKMRIDDAKGS
                     RRATKAASKLPAESTSEHPSVAVPEKLYFVDGYHGGAIFWDWLILPGGGLWRTYNWPL
                     CVRPVIKRALHDSRFKVVLDFDAYTYEWMAKHDSHATSLLRSALEAGALEIVNGTYGQ
                     PFGCAESGESFIRQLELGNRAIEAALGSSPEVFYSQEPTYFAQLPQILSLMGYSGAVF
                     RTQWAAFGTDPAHDEDLVTWVAPDGSEIPTVPRYSFQNYKRQLEHHPGLAAGSLGIGD
                     KPDWAPESFPAFLEAASACGIAHPFISDLKDVNLPEAPLPRATEIVAMKNIVFTRPKD
                     YLRIANPPQEKVSHGPHDTPCTLPWGLEADDVPRSVAAAESALRVAESLDAVLHFFKP
                     EYASAESSLTDAWKSLCIAQQHDLYVCGPWLSLAHRQPMWRVAVGFADHARKEADRIT
                     SEAMASISTGPYSGISASGDIHPSSGISSGISGGIIVFNAASYERRDYVEVKMPKAAL
                     PQDKPQGKSELCAYSNDELYSCQLISEDESEICLGFVPKLQGLSLKHFSLMQVPTQHS
                     ETEDSISPPFTILPTAKDGLTLCSSSGEKLIEGLCFTCVRDGKPYNSLLSTKTAKWVA
                     NGPVLYSLELEGNVADLPFILTLKMYSGVKRIDASVRFDFGDGPTYLGPQMDDYPQGT
                     PYSIHDEAKLCVSFNVPAECICCKSPFWISEVSGERITGAPWIGLESDERKHPGEHKR
                     EHLAFINRGNRGYHWNKEEHRLRQVLAWAPREWIYASDDSFTRGKSAFTALSGTRTFE
                     FAIAPYADRVEAERHSMNFATPFIAIWQDSSGAEASKSAPTSKTKPSDYIKSCSALEL
                     DSEKVFITAFLVQNGNIIARLWNASG"
BASE COUNT          805 a          833 c          805 g          809 t
ORIGIN      
        1 aagtggtccc ttgccccgag gatggcgagc ttgcacctct aggagaatat ggcagaaaca
       61 aaatcgaagc tgaaaatctc tgccgcgagg cttgcgcagc gggtcttcct gtcgtcatgt
      121 tgagacctcc aactattaca gggtctggcc tcgatgagcc ctttatattg agccttttgt
      181 ccgctatcca gaagggaaaa cccgtgaccc ttcttgggaa aggaaccaat agatttcaat
      241 tcattcatgt cgatgatgtt gtagatgcat gcttgctcgc tgccgagcat ccagatgcag
      301 ctggagaagc attcaatatt ggtggaaagg atgtgccaag catagctgaa ttggttcacc
      361 tagttgcctc tgcggtaggt tcgccatcga aagtgaggct cattcccaca acgccagcaa
      421 agatagccat agccctcttg agacctttcg gtgctgctcc attggagccc gagcatgtcg
      481 ctatagcgat ctgcgattat gttttcgatt ggtctaaagc acagaggata cttggctggg
      541 aacctcgctg gagtgtagcc gatgcactta tcgacacata tcgggctatt tatggagggt
      601 agttcatgag tctagtggat aatgcctcta aggctctagt ctcaggccgt attccttcac
      661 ctctggttcg cggcgcagct catgtagtcg tcgcgatcct tagcgccaaa atgcgtatag
      721 atgacgcgaa aggttcaagg cgtgcaacaa aagccgcctc gaaattgcct gccgagtcca
      781 cttcggaaca tccatctgta gctgtgcctg agaaactata ttttgtggat ggatatcatg
      841 gtggcgcgat tttctgggac tggctcattt tgccaggagg aggtctctgg cgaacgtata
      901 actggcctct ctgtgtgcga cctgtcataa agcgtgcgct ccatgattcc agattcaagg
      961 ttgtgctcga tttcgatgct tacacctacg aatggatggc aaaacacgat tcacacgcga
     1021 ctagccttct tcgcagcgcc ctggaggccg gtgcgctcga aatcgtaaat gggacatatg
     1081 gtcaaccatt tggctgtgca gagagcggag agtctttcat caggcagctc gaactaggca
     1141 atagagccat cgaggccgcg ctcggttcat caccagaggt attctattcg caagaaccaa
     1201 cgtattttgc tcagcttccg cagatcctgt ccctgatggg ctattcagga gctgtcttca
     1261 gaacacaatg ggccgctttt ggcacagacc cggcccacga tgaggatctt gttacatggg
     1321 tggctcctga tggcagcgag attcctacgg tacctcgata ttcctttcag aattacaagc
     1381 ggcagctcga gcaccaccct ggtcttgccg caggttccct tgggatagga gataaacccg
     1441 attgggcgcc tgaaagcttc ccggcttttc tggaggctgc ttcggcgtgt ggaattgcac
     1501 acccctttat ttcggatcta aaggacgtaa atcttcccga ggcgccattg cctcgtgcta
     1561 cagagatagt agcaatgaag aatatcgtat tcacgagacc taaagactat cttcgaattg
     1621 ctaatccgcc acaggaaaaa gtctcccatg gcccacatga tactccttgc acactaccct
     1681 ggggcctaga ggccgacgat gttccgagat cagtggccgc agcggaatca gctcttcgcg
     1741 tcgcagaaag tctcgatgcc gtgctccact ttttcaagcc tgaatacgcg agcgcggagt
     1801 catctcttac ggatgcatgg aagtccctat gtattgctca acagcacgac ctctatgtct
     1861 gcggtccctg gctttcgctt gcccataggc agcctatgtg gcgtgttgca gtaggctttg
     1921 cggatcacgc tcgcaaagag gcggatagaa taacctcaga agcaatggca agcatttcaa
     1981 ctggccctta tagcggtatt tctgcttctg gcgatatcca cccttctagc ggcatttcta
     2041 gtggcatttc tggtggtatc atcgtcttca atgcggcttc atacgaacgc cgcgattatg
     2101 tcgaggtcaa aatgccaaag gcagcactac ctcaagacaa accacaaggg aaaagcgaac
     2161 tttgtgctta ttccaacgat gaactatatt catgccagct catctcagaa gacgaatctg
     2221 agatatgtct tggttttgta ccaaagctgc agggcctctc cctcaagcat ttttctctta
     2281 tgcaagtacc tacacagcat agtgaaacag aagacagcat aagtcctcca ttcaccatat
     2341 tgcctacagc taaagatgga cttactttgt gctcttcttc tggagaaaaa ctaatcgaag
     2401 gcctctgctt cacctgtgtg cgcgatggca aaccttacaa ttcgctctta tctacgaaaa
     2461 cggcaaaatg ggtcgcgaac gggcctgttc tctattcact cgaactggaa ggcaatgtgg
     2521 ctgatctacc cttcatccta actctaaaaa tgtattcggg tgtaaagaga atagatgcaa
     2581 gcgttcgctt cgatttcgga gatggcccga catatctggg gcctcaaatg gatgactacc
     2641 cacagggcac gccctattcg atacatgacg aagcaaaact ctgtgtttcc ttcaatgtcc
     2701 ccgctgagtg catctgctgc aaatctccat tctggatatc agaggtctct ggtgagcgga
     2761 taacaggtgc gccatggatt ggcctcgaat ctgatgagag aaagcatccc ggagaacaca
     2821 agagagagca tcttgctttc atcaatagag gcaatagggg ctatcattgg aacaaagaag
     2881 agcataggct tcgccaggtc ctcgcctggg ctcccagaga gtggatctat gcaagcgacg
     2941 actcctttac acgaggaaaa tcagccttca cagccttgtc gggcacacgc accttcgaat
     3001 ttgctatcgc tccctatgcg gaccgagtag aagcagaaag gcactcaatg aactttgcta
     3061 cacccttcat agcgatttgg caagactcaa gcggagcaga agcatctaaa tctgctccga
     3121 caagcaagac aaagccctcc gattatatta agtcctgtag cgctctcgag ttggactcag
     3181 agaaagtctt cattactgcc ttcttggtac aaaatgggaa tatcatcgca aggctctgga
     3241 atgcttccgg ga
//
LOCUS       DAOOWY010000163         3231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_29852_length_3231_cov_9.070844, whole genome shotgun sequence.
ACCESSION   DAOOWY010000163 DAOOWY010000000
VERSION     DAOOWY010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29852_length_3231_cov_9.070844"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1881)
                     /locus_tag="PK105_08590"
     CDS             complement(76..1881)
                     /locus_tag="PK105_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61813.1"
                     /translation="MGNETDETMDLTETGDESESAASSSETNEDASSKSITQPRKGEV
                     VADENKRFLAEIVALRAMERISDAIAKSIVAEVREGKILIVNEMDFAGDEILYLDIRY
                     KLNTLLDLSKKANEECQKFIPHKEERAIKGLGKEAVVTQFAPPATLAAIPALIAGIPK
                     VPVFLGAIGDIVGLFRSDYELKGQTLTVPEDIYIQAIVADRLHRAKLEAYLIDFHSLK
                     ESILTRDLNYLIQQKFEMLACAEAIKNTVIDAKSGQTTALETYVAKLREKLADALLKA
                     DKNVIDILEKKIEDKIKTLDNYQRDRMPDTLINGLESYLASLRNKMAEELLKNDPKKS
                     IEELEFKIKEINEKLKDKTLKEDEVQSLNDQRKLYQTKMVDLVVNCNYNTKEILKVEI
                     DCIEKELEVLHKDRRPTTQINAIEAHIASLNTKLVDSLATNDEEIIDCLNREIDKWSN
                     EIKKIRESMREAQEIVGKCTKVNDAIDVFITSITNVPIGRNYSPYVIACAREYFAGDQ
                     FKYILKLKLITSGADFIIEKPAFWSNDVRTSYLGGGIVSFILAEKNGRIAASGTISRL
                     SALDHRIGDKPKEINWNIRPKYKKSEILSTGEEIQ"
BASE COUNT          830 a          736 c          682 g          983 t
ORIGIN      
        1 gatattaact ataaggaaat tagggattct aagtcaaagg tgcgaaatgt gggtttcatg
       61 cgtgttccag acagattatt gtatttcctc acccgtgctc aagatttcgg actttttata
      121 ttttgggcga atattccaat ttatttcctt tggcttatct ccaattctgt gatccaaagc
      181 tgataagcgg ctaatagtgc ctgaagcagc gattctacca ttcttctcag ctaagataaa
      241 gctaacaatc ccgcctccca ggtacgaagt acgcacatcg ttggaccaga aagccggttt
      301 ttcaattatg aaatccgcgc ccgacgtgat cagctttagc ttcaggatat atttgaactg
      361 atctcccgca aaatactctc tcgcacatgc tattacgtaa ggcgaataat tcctgccaat
      421 tggcacattt gtgatggaag ttataaagac atcaatggca tcattgactt tggtacattt
      481 gcctacaatt tcttgtgctt ccctcatcga ttcacgaatc ttcttgatct cattagacca
      541 tttatctatc tcgcgattta gacaatctat tatctcttca tcgttcgtag ccaggctatc
      601 aactagtttc gtattcaggc tggctatatg tgcctctatg gcattaattt gcgtagtagg
      661 cctccggtct ttgtgcaata cttcgagttc cttttctatg cagtcaattt caacttttaa
      721 aatttcctta gtattatagt tacaattcac aaccaaatca accatcttag tttggtaaag
      781 ctttcgctga tcattaagcg attgcacttc gtcttccttt agcgttttat ctttaagctt
      841 ctcattgatt tcttttatct taaattccaa ttcttctatc gattttttcg gatcattctt
      901 gagaagctct tctgccatct tatttcgcag gcttgcaagg tacgattcaa gaccatttat
      961 cagagtatct ggcatccggt ccctctgata attgtccaga gtttttattt tatcctcgat
     1021 tttcttttcc aaaatatcga taacattctt atccgccttc aacagggcgt cagctaattt
     1081 ttctcgcagt tttgccacat atgtttcaag ggcagttgtc tgcccacttt ttgcatcaat
     1141 gactgtgttc ttgatcgcct ctgcacaggc cagcatctca aatttctgtt ggatcaggta
     1201 attcagatca cgggtcaata tagattcttt gagtgaatgg aaatcgatta gatacgcctc
     1261 tagtttggct ctatgaagac gatccgcgac tatggcctgg atgtagatat cttcagggac
     1321 tgtcaatgtc tgtcccttaa gctcataatc ggagcggaaa aggccgacga tatcaccgat
     1381 agcacccaaa aagacaggaa ccttcggaat ccccgctatg agtgctggga tcgctgccaa
     1441 agtcgccggg ggagcaaact gagtaacgac tgcttcttta ccaaggcctt taatggctct
     1501 ttcctcttta tgcggaatga atttttggca ttcttcattg gctttttttg acagatctaa
     1561 gagggtgttc agcttatagc ggatgtccag atataagatc tcgtctcctg caaaatccat
     1621 ttcattgact attagtatct tgccctcgcg cacctctgca actattgatt tcgcgatagc
     1681 atcgcttatc ctctccattg cacgtaaagc gacaatttcc gctaggaatc tcttattctc
     1741 atcggctaca acttctcctt tgcggggctg ggttatgctt ttggaggaag catcctcgtt
     1801 tgtttcactg ctgctggctg ctgattcact ttcatcgccc gtttctgtca gatccattgt
     1861 ttcatcagtt tcattgccca tcgtttcttc ctccaaccaa gggcctaatt atttcttctc
     1921 acagcgaagc aatattggcc aaagttaata atcatagatt ataaattttt ctgagagccc
     1981 tctggcttat gaaggcagtg tgcccaaagt tctgtaattt caatcgccct ttatccaaca
     2041 tctcacccca cgtccataga tactgcgttc cgcacattcg actcgtgtat ccaactacca
     2101 ggtaatagaa ctgcaaatat caacgagagg aatagcccat atggccaata tgctggcaat
     2161 aggctcggcc catagggtca tgcgacagaa ttctggttaa ggtgtcggga aagcatatac
     2221 acttgttctt gcacatctca tcatggaagc actcgagaaa aaggatcaaa atcgatgtgc
     2281 tgcgaggaaa tcgaagattt ctgagccgcg ggattaacaa gaagctttcc tggatttgcg
     2341 cttaacccat ctgtgcacaa ggcggagcat atccagagta acctcggcct agatttaggt
     2401 ccaggttcaa tttcgagtat agctaagact ctagtaacgg caggctgaac gcgctacgat
     2461 ttccctaatt ataagacgct ctggtgaatt cacttgaact attttgaaac tccatcatat
     2521 tgatggagtt agtaagatcc tcattgccct gatgttatat aattactcgt aagtacgcgc
     2581 agtattcatg tcccccctac ccaaacatat cgtttttgtg tctttgattt tcggcaaact
     2641 gcttggagtt tggccaggcc aagcgggaga gccagagaac cgttgagcgg tacttggagg
     2701 ccctttacga agcaggattg gcttgtcgag agcagatgac cgcaccggga aaaccgtggg
     2761 gatactggtg tgaggaagaa ttgcgacaaa aggtcctgtc gaaaatatcg atatcggaag
     2821 gcgatgcgac gaatagcgac agattcacga caaaatcctt atgtcgcaaa tatatggccg
     2881 aaaaatcgtc cgattcgtta aaggattcga ttaaagagtt cttttctaat agcgacataa
     2941 taggtcagga aatgtatagg ggaatttgga gcgggagtga ccttctgggc ggagatcccg
     3001 agtcgctcta tctttctctc ttttccctga aaaagtgtcg caattcagaa aatgcgcctg
     3061 ttgattgcgg ttaattgcga cagggcgaaa tgtagctatt gtcggaaatg cccaatgata
     3121 gcgaatttga aaatgtcgca tgagatgtcg caatgcagca accgctcgaa aaggctatag
     3181 tcaaacggcg gcggcgcacc ccgcagcaga gctgcggggc atgcgaggcg c
//
LOCUS       DAOOWY010000164         3221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_29956_length_3221_cov_11.411244, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000164 DAOOWY010000000
VERSION     DAOOWY010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29956_length_3221_cov_11.411244"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..675)
                     /locus_tag="PK105_08595"
     CDS             complement(187..675)
                     /locus_tag="PK105_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HOU61814.1"
                     /translation="MKEKIIIVAAILVGAWIGSKLKLPSGYLTGGLILGLIVKGIVGG
                     NVPSSGSVSIISQLLVAYVLVATSNVESLKQRPEVIPVALGYVVALLAFTTGLSFILN
                     RFFHIDLLTAMYSTAPGGISGMAITAVEAGADTAISSAFQVLRIVLVLVATPILAGIL
                     SK"
     gene            complement(698..2260)
                     /gene="citF"
                     /locus_tag="PK105_08600"
     CDS             complement(698..2260)
                     /gene="citF"
                     /locus_tag="PK105_08600"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOU61815.1"
                     /translation="MSTNCVRNSLGRLVPREIRGLGAVEPFSGPYARLDGSFIWTASP
                     LSRRVPTISANKVVSSLRAAIERSGLQNGMTISFHHHLRNGDAIVSLVLSELEKMGYR
                     DLTLAPSSLGDSHDCVADAIKKGLVTRLNTSGVRGEVGKAISNGYLESPAIIRSHGGR
                     ARAIEEGSLKIDVAFLGAPACDRLGNMTGVIGNTACGSLGYAMVDARYASHVIAITDN
                     LVDYPLSPRISIPQYLVDQVVVVDSIGDPSKIATGATRITRDPLQLYIAELAFKVIKA
                     SGYLKNDCSFQTGGGGPSLAVARYVRDYMKSNSIKGSYCIGGITGYMADMLKEGLFRT
                     LFDVQSFDAAVIDSIAHNPNHVEADASWYANPFNKGCLVNDLDVVVLAALDVDIDFNV
                     DVLTGHDGILRGASGGHSDTAAGAKLALITIPSMRNGVPSIRERVQTIVTPGETIDVI
                     VTERGIAVNPRREDLIAKLKDSGLPIKNIHQLKKDIEAITGIPEEISFQNEIVGIVEY
                     RDGTIIDSIRKV"
     gene            complement(2257..3159)
                     /locus_tag="PK105_08605"
     CDS             complement(2257..3159)
                     /locus_tag="PK105_08605"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOU61816.1"
                     /translation="MSKAFQNHSMRSLLYIPGNNPSMLQNCAIYGADAVIMDLEDSIS
                     ISEKDSARNLVKHALRDLDFGDMSIIVRVNARDTPYFEQDIEEIIPASPDAIRLPKVD
                     SASDVLAADELISRLEEINGLPKGKIGIQAMLETARAIINVNSIAASSPRLVGLTLGG
                     QDLAADLGIRATKEGYELIYAKSAVIIAAKAFGLQAFDTVFTDVNDPEGLRTQTELSF
                     ALGFSGKAAIHPSQVSIINSVYTPDPKELAKAIKIVTAAREAEQKGQGVIAVDGRMVD
                     APVVSQALRILELARLAGMEVSEL"
BASE COUNT          804 a          799 c          741 g          877 t
ORIGIN      
        1 ggaggcggtg atcgccgatg tcggcgtgtc gctcttggct gtcatgaatt cgctgcgcac
       61 tatagggcat aaattttcat gaatttgcag cgtccaaaga ccactgacca aaggtcacag
      121 accatggatt ttccccttgt gcatataagg atcttgataa gggtcttgat ggctttctct
      181 ctttaattat tttgacagaa tccctgctaa tataggcgta gcgacaagaa cgagcacaat
      241 tctcagcacc tgaaatgccg aggatattgc cgtatctgct ccggcttcca cggcggtaat
      301 agccattccg cttatgccgc ccggtgctgt gctatacata gctgtcagca agtcgatgtg
      361 gaaaaaccta ttgagaatga aagataaacc agtggtgaat gccaaaagcg ccacaacata
      421 tccaagagcc acaggaatca cttcgggtct ttgttttagc gactccacat ttgatgttgc
      481 cactagaaca tatgccacaa gaagctgcga aattattgag acagatccac tgctgggaac
      541 attgccgcct acaattccct tgacaataag gccaaggatt aaccctcctg taaggtagcc
      601 agaaggcagc ttgagtttgc ttccgatcca agctcctacc agaattgcgg caacaatgat
      661 tatcttttct ttcataatgc ctccggcggc gcttttatca aacttttcgt attgagtcta
      721 taatagtccc atcgcggtat tccactattc cgacgatttc gttttggaag gaaatttcct
      781 cgggaatgcc cgtaatagcc tcgatatctt tcttcagctg atgaatgttc tttataggaa
      841 ggccagaatc ctttagttta gcaatgagat cttctcgtct gggattcacg gcaatacctc
      901 tctcggtaac gatgacatcg atggtttctc caggagttac tatggtctgt actctttccc
      961 gtatggaagg gacaccattt cgcatagaag gaattgtgat cagagcgagc tttgccccag
     1021 cagcggtatc cgaatgacct ccggatgctc cgcgcagaat gccatcgtgg cctgttaaga
     1081 catcaacatt gaaatcgata tcgacatcga gggctgcgag tacgactaca tcgagatcat
     1141 tgacaagaca gcctttatta aatgggttgg cgtaccatga ggcatctgct tccacatggt
     1201 tcggattgtg tgcgatggaa tcgattacgg cagcgtcaaa gctctgtaca tcaaatagtg
     1261 tcctaaagag gccctctttg agcatatctg ccatatagcc cgttattcct cctatgcaat
     1321 agctgccttt tatggagtta gatttcatat aatcccgcac atagcgtgca actgcgagac
     1381 ttggtccgcc tccgcctgtc tggaacgagc agtcattctt caaatagcct gaagccttga
     1441 tgaccttgaa ggcaagctca gctatataca actgtaatgg atccctggtt attctggtgg
     1501 cacctgttgc tattttggaa gggtcgccta tggaatcgac cactaccacc tgatctacga
     1561 gatattgcgg aatgctgatt ctgggggaca gaggatagtc gacaagattg tcggttattg
     1621 caatgacatg gctagcgtat cgagcgtcca ccatggcata gcctagagag ccacatgctg
     1681 tgttgccaat tacgccagtc atgtttccaa gtcggtcgca agctggtgca cccaggaatg
     1741 cgacatcgat tttaaggctg ccttcttcga tagctcgcgc tcgtccgccg tggcttcgta
     1801 ttatcgcagg gctctccaga tagccattcg aaatggcctt gccaacctcg cctcggactc
     1861 cagaggtatt cagtctcgtt accagtccct tctttattgc atccgccaca cagtcgtgtg
     1921 agtctcccag agaagagggc gcaagtgtca ggtctcgata gcccattttc tccagctcgg
     1981 acaatacaag gcttactatt gcatcgccat ttctaagatg gtgatgaaaa gaaattgtca
     2041 taccattctg caaaccggag cgctctattg ctgccctcag gcttgagact actttattgg
     2101 cactgatagt cggcactctt cgagagagcg gagaggccgt ccatataaat gaaccatcta
     2161 gtcttgcata aggtcctgag aatggttcta cagcccccag accgcgaatc tctctaggaa
     2221 cgagcctgcc aagcgagttg cgtacacaat tggtgctcat agttctgaca cctccatccc
     2281 agctaaacgt gcaagttcga gaatccttag cgcttgtgac acaaccggag catctaccat
     2341 gcgtccgtct accgctataa ccccttgccc tttttgttcg gcttctctag cggctgtgac
     2401 gatcttgatt gcctttgcaa gctcttttgg atctggagta tagacagagt taataatgga
     2461 gacctgcgat ggatgtatgg cggcttttcc actaaatcca agagcaaagc tgagctctgt
     2521 ctgggttcgc agaccttcgg gatcattgac atctgtaaag acagtatcga aggcttggag
     2581 cccaaatgct tttgcagcga tgatgacagc gctcttcgcg taaattagct cataaccctc
     2641 ttttgtggca cgtatgccaa gatcggccgc caaatcctgc ccacctagag tcagtcctac
     2701 gagacgtggt gatgaagcag caatcgaatt tacattgata attgcgcgtg cggtttcgag
     2761 catggcttga atgcctattt tgccctttgg cagaccgttt atctcttcca gtctggatat
     2821 caattcatcg gcggcgagca catcagacgc cgaatctact ttaggcaaac gtattgcatc
     2881 tggactagcc gggataatct cttcgatatc ttgctcaaaa tagggagtgt cccttgcatt
     2941 tactcgaact attatgctca tatcgccaaa atctaggtct cgaagagcat gttttacaag
     3001 gttgcgcgct gaatcctttt cagaaatgga aatcgaatct tcgagatcca ttataacggc
     3061 atccgcgcca tagattgcac aattctgcag catactcggg ttattgccag ggatgtagag
     3121 cagagaacgc attgagtgat tctgaaatgc cttactcatt ttgcaacttg tcttctcctt
     3181 tttaccaaag catatacaag tgatacacca gctaacacaa t
//
LOCUS       DAOOWY010000165         3220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_29970_length_3220_cov_12.796840, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000165 DAOOWY010000000
VERSION     DAOOWY010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29970_length_3220_cov_12.796840"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK105_08610"
     CDS             <1..430
                     /locus_tag="PK105_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61817.1"
                     /translation="VLPVAANIIFNSYHWEIPFANDGSTPYQNDQQSVMTVRSGSTWI
                     FHLEFQSTNLGYLKQGEDKIPYFIKVTEKDNSYGGIILPSAIMNGYVQLTSAQKIYFI
                     GKTPKNGSQFYVGIKINALPGEFYESGAYTDTLTVSFFVL"
     gene            complement(510..875)
                     /locus_tag="PK105_08615"
     CDS             complement(510..875)
                     /locus_tag="PK105_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61818.1"
                     /translation="MQKLFPTSSISCKGVDIFRAILSEAASSSWDSDRYTLLLSMTKK
                     KNASAQRKSESKTFGALELLFFGLLIGLIIQWEKHIMRKTFMPWAEINPKQIPSFSIT
                     ELLEKCFLGNLYKVLAKRS"
     gene            856..2070
                     /locus_tag="PK105_08620"
     CDS             856..2070
                     /locus_tag="PK105_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU61819.1"
                     /translation="MGNNFCKLLLTDSGRTYAALKPLAEESLSRLPYIDSITIAPGAI
                     IRYFFPEDRASASIGHDLLDNPERMNTLVNAVRKRKAALQGPDISAEGKTLAFLRIPV
                     FEGEELWGFVSIAFDTDKVLGNLDLPSEFPGLSIALVSSRMDGGEKLVFWGEVRALSG
                     YSAVVEIESEDFPWIVYVASSYPYRRVVAWGAGLLILVLVSCGLFILEEFSEKESKSH
                     GRPKEASMDIKPFVPGSESAQKLSMGVSTKAENEAAQLIEEPARVSLEEKSNCISVLI
                     VDDSEVNRDLLLRMLTLKGYEARAVSSAEASLESLKVKSFDIMLIDCVMPEMDGYALA
                     QKIRAEDTSHQKGLQSALPRPVLIAMSPRHDQEEAERCAKAGFDSLLVKPFTMTALDQ
                     QIRLILDDKRIG"
     gene            2435..2917
                     /locus_tag="PK105_08625"
     CDS             2435..2917
                     /locus_tag="PK105_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61820.1"
                     /translation="MKKYALVALFAVVSLGMVFAVVAVPPTQGNISITGEVKEAFSLL
                     VPPDYKGTIENGDVAETWQIGNVAVTSNVNNWTIKLTSKTGGVLLHSSEKDEIAYTVT
                     LEGLVKDQSLKNEWQSKPQGRTSKGGNSYPFSITFGPSTNYYEAGFYNDVITVTISHS
                     "
     gene            complement(3014..>3220)
                     /locus_tag="PK105_08630"
     CDS             complement(3014..>3220)
                     /locus_tag="PK105_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61821.1"
                     /translation="ERTAQHRISIHELKVQKNGEGNKDLREELHQIYKEHKKAARMKI
                     GAKSWHIYKSKARSKKQEPQQYQR"
BASE COUNT          881 a          666 c          750 g          923 t
ORIGIN      
        1 ggtgctacct gtagcagcta atattatttt caattcttac cattgggaaa ttccattcgc
       61 aaacgatggc tctacacctt atcaaaatga tcagcagagc gtcatgacgg taagatcagg
      121 gagtacttgg atttttcatc ttgaatttca atcgacgaac cttggctacc ttaagcaagg
      181 tgaagacaaa atcccttatt ttatcaaagt gacagagaaa gataatagtt atgggggtat
      241 aatattacca tccgcgatta tgaatggata tgtacagctt acaagtgcac agaaaatata
      301 ttttatcggc aagaccccca agaatggttc tcaattctat gtgggaatca agatcaatgc
      361 cctgccaggt gaattctacg agtctggtgc ttatacagat accctgacgg taagcttttt
      421 tgtattatag aaatagcctc aagtgcgaaa attcgaggtt cgaaaacatc catatgctga
      481 tgtatatagc tccttttttt ggctttccat caagatcttt ttgcaaggac tttgtagagg
      541 ttgcctagaa agcacttttc aaggagttct gttattgaaa aagagggaat ctgttttgga
      601 ttgatttccg cccatggcat gaaggtcttt ctcatgatat gcttttccca ttgaattatg
      661 agcccaatta gcagtccgaa gaagagcaac tccagggcac cgaaggtctt gctttcgctc
      721 ttcctttgcg cacttgcgtt tttcttcttt gtcatgctca aaagaagcgt atatcgatct
      781 gagtcccaac tacttgaggc tgcctctgag agaatagccc tgaaaatatc gacgcctttg
      841 caagagatgc tcgaggtggg gaataatttt tgcaagctgc tccttaccga ttcgggtaga
      901 acatatgctg ctctaaaacc actcgctgag gagtctcttt cgcgcttgcc ctatattgat
      961 tcgattacga tagctccggg cgcaattatc cgatatttct ttccagagga tagagcttcc
     1021 gcttctatag gccatgatct gctcgacaac ccagaaagga tgaatacgct ggtcaatgcg
     1081 gtgcgcaaaa gaaaggcagc actccaggga ccagacattt ccgcagaagg gaagactctc
     1141 gcattcttga ggatacccgt gtttgaaggc gaggaactct ggggctttgt gagtatcgct
     1201 tttgataccg ataaagtctt ggggaacctt gatcttcctt cagaattccc gggactatct
     1261 atcgctcttg tctcttctcg tatggatggc ggagagaagc tagttttttg gggtgaggtg
     1321 cgcgctcttt cgggatattc agcggtggta gaaatcgagt ctgaggattt tccatggatt
     1381 gtctatgtcg catcgagtta tccatatagg cgagtcgtgg catggggagc agggcttctt
     1441 atactagttc ttgtgagttg cggacttttc atactggaag aattttcaga aaaagagagc
     1501 aaatctcatg gacgccctaa agaagcttcc atggatataa agccctttgt gccaggatca
     1561 gaatcagcac aaaagctttc tatgggagtt tctaccaagg ctgagaatga agcagcgcag
     1621 ttgatagaag agccagcgcg cgtctcgctc gaagagaaat caaattgcat atcagtgctc
     1681 atcgtcgacg atagcgaagt aaaccgcgat cttcttttgc gaatgctcac attgaaaggt
     1741 tatgaggcaa gggctgtctc aagtgcagaa gcatctctag aatcgctcaa ggtaaagagc
     1801 ttcgatatta tgctcataga ctgtgttatg cccgaaatgg atggttatgc tttggcccag
     1861 aagattcggg ctgaagacac atctcaccaa aaaggcctcc agagtgcttt gcccagacca
     1921 gtactcatcg ccatgagccc gcgccatgac caggaagaag cggagagatg tgctaaagct
     1981 ggcttcgaca gtcttctagt taagcccttt acgatgacag ccctcgatca acaaattcgc
     2041 cttattcttg atgataagag aataggatga gggcttgcct tccaatgaca gctttcctta
     2101 tctggcagaa tgcatcttta gtgtcgtcat tgaaaaacaa taattaagtt tatagagata
     2161 ttagacaaaa gtggcaattt ctctattcta ttaaatagct acaatttaca gtgtacatat
     2221 taaattgcta attcacgatt aaatcaaaaa ttatcaatgg tagtaagaga gaccaaaagt
     2281 tctaagctct taaatataga gaaaaactca ttgcacaaga tgtttatgtc gattataatt
     2341 atatcaaagt gtagtggcgg aatccgttgg aagaagccac aaaagacaca aaatgtatcc
     2401 gtggggcata gaccctgcgg gcgggagacg caaaatgaaa aagtatgcgt tggtagctct
     2461 gtttgcggta gtgtcgttag ggatggtgtt tgctgtagtg gctgtgcctc ctactcaggg
     2521 caatatttcc attactggcg aggtaaaaga agcctttagc cttttagtac ctcctgatta
     2581 taaaggtact attgaaaatg gagatgtggc cgagacatgg caaattggaa atgttgcagt
     2641 taccagcaac gtcaacaatt ggaccatcaa gttgacgagc aaaaccggag gcgttctgct
     2701 tcatagcagc gaaaaagatg agattgctta cacagtgacg ttagaggggc tcgttaaaga
     2761 ccaaagcctg aagaatgaat ggcagagtaa gcctcaaggc aggacttcaa agggaggtaa
     2821 ttcctatcca ttcagcatca cattcggtcc gtctacaaat tactatgagg ctggcttcta
     2881 taatgatgtg atcaccgtca ccatcagcca tagctgatcc gcagcagagt gatgacagga
     2941 aggccgtcca ggctaaggcc gggcggcctt tcatttttta gcagaaatta ggccttattc
     3001 ttgttttggg cagctatcgc tgatattgtt gaggttcttg ttttttgctc ctagccttgc
     3061 ttttgtaaat gtgccagctt ttggcgccta ttttcattct tgccgccttt ttatgctctt
     3121 tgtatatctg atgaagttcc tcgcgcaagt ccttgttccc ttccccgttt ttctgtactt
     3181 ttaactcatg aatcgaaatt ctgtgctgcg ccgtcctttc
//
LOCUS       DAOOWY010000166         3115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_31311_length_3115_cov_24.319608, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000166 DAOOWY010000000
VERSION     DAOOWY010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31311_length_3115_cov_24.319608"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK105_08635"
     CDS             complement(<1..413)
                     /locus_tag="PK105_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61822.1"
                     /translation="MANQFELIGDDSLFSNDQSDGDDTDNKKANKNEVTCLGKSFPDD
                     EAGRAYFTEELRKKLKDPEFRKIEGFPLGSDEDILALSDPPYYTACPNPWIDEFVRTW
                     EEEKPPKPDGYVYHREPFAADVSEGKNDPIYNAHS"
     gene            complement(453..>1304)
                     /locus_tag="PK105_08640"
     CDS             complement(453..>1304)
                     /locus_tag="PK105_08640"
                     /inference="COORDINATES: protein motif:HMM:NF039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HOU61823.1"
                     /translation="NLINDQYTFTNYLSQAAEILPTDNPWRKKYEKEKEEILNLYRTL
                     PIDELNGSSRELSMRLQELKNEYKKAYIELHSQNRLGRSEDEKKRNLTSDRRFYILQK
                     LTSIELLQNNQFVAWKENLAKLKTCFSLSPEQLDKIPYCQACEFRPVAEPEIHPSAKR
                     QLIELDSKLDEILEAVTHTLITNLSDPIIQQNLELLKPDEHEMINNVVMTREIPEVVD
                     SNFVNALQHALSGLVRIPLRISDLATALKLTEGPTTVDELHQRFEDFLEELTKGKDPN
                     KIRIVME"
     assembly_gap    1305..1404
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1405..>3115)
                     /locus_tag="PK105_08645"
     CDS             complement(<1405..>3115)
                     /locus_tag="PK105_08645"
                     /inference="COORDINATES: protein motif:HMM:NF039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HOU61824.1"
                     /translation="DRFEQVIIARNDVKFVVAERLLKKNAEQQHLVREHLEKFSRFFS
                     SLSERMEEFVSLFPIHPDYIDTFERIKIAEKREVLKTLSLGIREFLEQDVPEDEPGLI
                     AFDAYWKNICEDASFRGMPDVRQVIDISKRIEDRIILGSINQLYKERALRIIHALSVE
                     RLTTGDIYAPVGSTPEELRDRLCLYDKNAAELGGKEPDKDLLTHVETVLREIIKAVNG
                     QFISYNPQNRQYYLDLKKTEDFDENIRIRAESLDNDTLDRYYYQVLRIAMECQDIETW
                     VPHYLIWQHELEWLGRNVTRLGYLFFGAPNERSTAVPPRDFYLYFLQPYDPPSFRDEK
                     KPDEVFFRLKSPDEFFIENLKLFAGANELALTSSAQPKQIYTGKAETFRKNLHEWLTK
                     NIDTAFEVTYRGQSKPMSQWMKGKSIRDLAGIGVDQTINFRDYVNSIASLCLSPYFED
                     QAPNYPHFSIKITTKNRAQAAQEALRAIAQNPKQSFTRQARAVLEGLMLIDNDRIDPS
                     KSPYANYILDKFANKSPGQVINRNEIIERVHDVDFMDPSQSRLEPEWVAVILASLVYS
                     GQIV"
BASE COUNT          775 a          638 c          623 g          979 t
ORIGIN      
        1 taggaatgtg cattgtaaat tgggtcgttt ttcccttcgc tcacatccgc tgcaaaaggc
       61 tcacggtggt acacataccc atctggtttt ggcggttttt cttcttccca tgttcttaca
      121 aactcatcaa tccatggatt aggacacgct gtgtagtagg gtggatctga gagcgccaaa
      181 atatcctcat cagaacctaa cggaaagcct tcgatcttgc gaaattccgg atctttgagc
      241 ttctttcgca gctcttcggt aaaataggca cgccccgctt cgtcgtctgg aaacgatttt
      301 cctaaacaag ttacttcatt cttattcgct tttttattat ctgtatcgtc tccgtcggat
      361 tgatcgttgg aaaaaagtga atcatcaccg ataagctcaa attggttagc catacttgta
      421 ttccttatta tgtttgattc gttatttctt agttattcca tcacaattct gatcttgttt
      481 ggatctttgc cttttgtaag ctcctccaag aaatcctcaa agcgctggtg tagttcgtcg
      541 actgttgtag ggccttctgt aagctttagt gctgttgcaa gatcagaaat gcgaagcggt
      601 atccgaacta agcccgataa agcgtgctgc aatgcattga caaaattgct atccaccact
      661 tctgggatct ctctagtcat tacaacatta ttgatcattt catgctcatc gggtttaaga
      721 agctctagat tctgttgtat tattgggtct gatagatttg ttatcaatgt atgagtcaca
      781 gcctctagaa tttcatcaag cttcgaatcg agctctatca attggcgctt tgcagagggg
      841 tgaatttcgg gttcagcgac aggtctaaac tcgcaagctt ggcaatatgg aattttatca
      901 agctgctctg ggctgagact gaagcaggtc tttaattttg caagattttc cttccatgca
      961 acaaactgat tattctgtag cagctcaatg ctagtgagct tttggagaat gtagaatcgt
     1021 ctgtcacttg ttaggttccg ctttttttcg tcttccgagc gacctaatcg attttgggaa
     1081 tgcaattcga tatatgcttt tttgtactca tttttaagtt cctgcagccg catgcttagt
     1141 tctctcgatg agccattcag ttcatctatt ggtaatgttc gatatagatt gagaatttct
     1201 tctttttctt tttcatattt tttgcgccaa gggttatctg tcggcaggat ttctgcagcc
     1261 tgagaaagat aattggtgaa ggtatattga tcgttgataa gattnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnactatt tggcctgaat agacgaggct tgcaagaatc
     1441 actgcgaccc actcaggctc tagtcttgat tgtgaaggat ccataaagtc aacatcatgc
     1501 accctttcga taatctcgtt tctgtttatg acctggccag gactcttgtt tgcaaattta
     1561 tcgaggatgt agttcgcata gggcgatttt gatggatcaa tgcgatcatt atcgatcagc
     1621 ataaggcctt caagcactgc tcgcgcttgg cgagtaaacg attgtttcgg attttgtgca
     1681 atggcacgca gcgcttcttg tgcagcttgt gcacggttct ttgtggtaat cttaatgcta
     1741 aagtgcggat aattcggcgc ttggtcttca aaataaggcg ataagcacag agaagcgatg
     1801 ctattcacat aatccctaaa gttaattgtc tggtctactc caattccagc aagatcgcga
     1861 atcgacttac ctttcatcca ttggctcatc ggttttgact gaccacgata ggtaacttca
     1921 aatgcggtgt ctatattttt tgtaagccat tcatgcaggt ttttgcgaaa agtttctgcc
     1981 ttgcctgtat atatttgttt tggctgagca gaggatgtta gagcaagttc atttgctcca
     2041 gcaaagagtt tgaggttttc tataaaaaat tcatcagggc ttttgaggcg aaagaacacc
     2101 tcatcgggct ttttttcatc tctaaatgaa ggaggatcat acggctgcag aaaatagagg
     2161 taaaaatcac gcggtggaac agcagtcgaa cgctcattag gagcaccaaa aaagagatag
     2221 ccaagtctgg tcacattgcg gccgagccat tcaagttcat gctgccaaat gaggtaatga
     2281 ggaacccatg tttctatatc ttggcattcc attgctattc tcaagacttg gtaatagtaa
     2341 cggtccaaag tatcattatc gaggctttca gctctaatcc ttatgttttc atcaaagtcc
     2401 tctgttttct taaggtcgag ataatactgc ctattttgtg ggttatagga aataaattgc
     2461 ccattgacgg ccttgataat ctctcgaagc acggtttcaa catgcgtgag gagatcttta
     2521 tcaggctcct ttcctccaag ttctgcagca ttcttatcat agaggcaaag tcgatctcgc
     2581 aattcttcag gcgtactacc aaccggcgca tagatgtcgc cagtagtgag cctttctaca
     2641 gagagcgcat gaataattct taatgccctt tctttataca gttgattgat agaacccaat
     2701 attattctgt cttcaattct cttacttata tcaatcacct gacgtacatc cggcattccg
     2761 cggaatgagg cgtcctcgca tatattcttc cagtaggcat caaaggcaat tagtccaggc
     2821 tcatcttcag gaacatcttg ctcaagaaac tctctaatac cgagcgaaag cgttttaagc
     2881 acttcgcgct tttctgcaat ttttatacgt tcaaaggtat cgatgtagtc tggatgtata
     2941 ggaaaaaggc tcacaaattc ttccattcgt tcacttaaag agctaaaaaa tcgactgaat
     3001 ttttcaagat gttcacgcac aagatgttgc tgctcagcat ttttcttcaa cagacgttca
     3061 gcaacgacaa atttgacatc attgcgcgca ataattacct gctcaaatct atctt
//
LOCUS       DAOOWY010000167         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_31488_length_3101_cov_7.542679, whole genome shotgun sequence.
ACCESSION   DAOOWY010000167 DAOOWY010000000
VERSION     DAOOWY010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31488_length_3101_cov_7.542679"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PK105_08650"
     CDS             complement(<1..501)
                     /locus_tag="PK105_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU61825.1"
                     /translation="MQVSGFRASTNLKRLARDPVLLAVILLVTASLAIFILYPLVEVA
                     ATSLDVGGGRLGLGVYSEVAESAYIRKALYNSLFMSTLTAIVGTIIGFIFAFAVVRAK
                     VPLARFFNTIAVIPIVSPPFIGALAIIMLFGNNGLISWNLLKIQNFPIYGFNGLFIAQ
                     VVTFFPV"
     gene            complement(509..1516)
                     /locus_tag="PK105_08655"
     CDS             complement(509..1516)
                     /locus_tag="PK105_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61826.1"
                     /translation="MKAIRKAMLVLAFVMLLVPAFAKAGQSIVVYSSVDEVNAKKILD
                     AFTAQTGIAVQFVHLSSGPAYARIKAESANPQADVWFGAPSENHIVAKNDKLTQPYKG
                     PNFDKLGKEFKDPEGYWRSFYMNPMAFAVNKEVIARIGAPKPTSWADLLNPAYKGQIQ
                     MPSPQSSGTAYNIVASLVVLMGEDKAFDYMKKLAPNIQTYTSSGTAPSQAVQVGQCAI
                     AIQFTPAFFEAIDKGFPIEVIFPKEGVWFEAPAISILKGARNLEAAQALVDWLSSVEG
                     QKVYTELKTYFYPVIPGVPLGKGMPAFESLKTIDVDVLWAGEQKKRLVERWVNEVLSA
                     K"
     gene            1704..3017
                     /locus_tag="PK105_08660"
     CDS             1704..3017
                     /locus_tag="PK105_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOU61827.1"
                     /translation="MSNQKAQVSQKGSKAAYRWDMVFAGAMLMLCFGTVYAWSYFQVP
                     LMQTFGWNNAQVSLVFSLAIFFLGMAAAVGGVILPNAGPQRIALIGSVLFSLGYLLAA
                     FALSLKSLALLYIGYSIIGGTGLGLGYVTPVATVSKWFPEKKGLATGMVIMGFGFGAL
                     FMSKVIAPVFLRLYDGNLVNSFLTFALLFAAITIPSSLAMKDPPERALGQSKQPNSSN
                     SRRQKQDNAKTAISLSLRQASKDIFSKRFLVVWMILFCNISAGISIIGFQSPMFQELI
                     INRNPSSTAEALASMGATLIAVTSIFNGVGRLLWGIFSDRFGRIRAYRLLLGSEILVF
                     LLLIFTRSPWVFAICACWILFCYGGGFGMMPATISELFGPQKMAIMYGAVLTAWSFGG
                     VIGPQITAYIKDTQPSQAIKISFIVGAVFIAIAFLFSILITDKKK"
BASE COUNT          831 a          708 c          694 g          868 t
ORIGIN      
        1 tacggggaaa aaggtgacta cctgagcaat aaaaagtcca ttgaatccgt aaataggaaa
       61 attctgaatc ttgagaaggt tccagctaat tagcccatta ttaccaaaaa gcataatgat
      121 cgccaaagca ccaataaatg gtggagatac tatgggaatc acggctatag tattgaagaa
      181 tcttgccaat ggaactttgg cacgcacaac cgcaaaggca aaaatgaagc caataattgt
      241 cccaacaatc gccgttagag tactcataaa caggctgttg tataaagcct tgcgaatata
      301 ggctgactct gcaacctcag aatatactcc gagcccaagc ctgccaccgc caacatcgag
      361 gctggttgca gctacttcga ccaaaggata aagtatgaat attgcaagcg atgcggtgac
      421 cagcaagata acggctagta acaccggatc gcgggcgaga cgcttcaaat tagtgctcgc
      481 ccggaatcca gatacctgca tcaattgctc actttgcgct taagacttca ttaacccagc
      541 gctctaccag ccttttcttc tgctcgcccg cccagaggac atcgacatcg atggtcttaa
      601 gagactcgaa agccggcatt cccttaccga gcggcacacc cggaataact ggatagaaat
      661 atgtctttag ctctgtatag accttttgtc cttcaacaga agagagccag tctactaaag
      721 cctgagctgc ttcgagattc ctggcacctt tgagtatgga gattgctggg gcctcgaacc
      781 agacaccttc ctttgggaaa atgacttcta tagggaagcc tttatcgatg gcttcgaaaa
      841 acgctggggt aaattgaatt gctattgcac attgtcctac ttgaacggct tgtgatggtg
      901 ctgtgcccga agatgtatag gtctgaatgt ttggggcaag ctttttcata taatcgaatg
      961 ccttgtcttc gcccataagc acgacgagcg aagcaacgat attatatgcg gttccagaag
     1021 attgaggaga tggcatctgt atttgcccct tgtacgctgg gttgagcaaa tcagcccaac
     1081 tcgtgggttt tggcgctcct atgcgagcaa taacttcctt attcaccgca aaagccatgg
     1141 gattcatgta gaagctacgc cagtatccct ccggatcttt gaactcctta cccagtttgt
     1201 caaaattcgg ccctttgtat ggctgagtga gcttatcatt ctttgccaca atatgatttt
     1261 cgctaggagc accgaaccac acatccgctt gaggatttgc ggattcagcc ttgattctgg
     1321 cataagctgg gccagaagat agatgaacaa actgcaccgc gataccggtc tgggcagtaa
     1381 aggcatccag aatctttttc gcattcacct catctacgct cgaatacact actatggact
     1441 gaccagcctt tgcaaaggcc ggtactaaca acataacaaa tgccaagact agcattgcct
     1501 tgcgaattgc tttcatagga tcgcctccag attaggaata taatctcata ttttataatc
     1561 tttttactgt gtccgcaatg agaaagagcc tatttagaac acgtaagtta ggcttcccca
     1621 tatcacaacc agccatttgc ttggcattga ctggacatga attatggcgc attctggatg
     1681 gcggttgcag aaggaataga atcatgtcaa atcaaaaagc acaagtctca caaaaaggtt
     1741 ctaaggctgc atatagatgg gacatggtct ttgcaggggc tatgttaatg ctctgctttg
     1801 ggacagtcta tgcttggagc tacttccaag tgcctcttat gcagaccttt ggatggaata
     1861 atgctcaggt ttcgcttgtt ttttcgttgg ctatattctt ccttggcatg gctgctgccg
     1921 taggtggggt tatacttcca aatgctggcc cccagagaat tgcactgatt ggaagcgttc
     1981 tttttagtct tggatacctt ttggcagcct ttgcgctttc gctcaaatcc ttagctttat
     2041 tatatatagg atatagtatt ataggaggca cgggacttgg gctcggatat gtaacaccgg
     2101 tggctacggt ttccaaatgg ttccctgaga aaaaggggct tgcaaccggc atggtaataa
     2161 tgggatttgg cttcggtgcg ctttttatga gcaaggtcat agcaccagtt tttctgcgat
     2221 tatatgatgg aaatctcgta aattctttcc ttacttttgc attgcttttt gctgctataa
     2281 caatcccttc atctcttgcc atgaaagacc caccagaaag agctcttgga caatcaaaac
     2341 agcccaattc ctccaattct cgccgacaaa agcaggacaa cgcaaaaaca gcaatttctc
     2401 tatctttaag acaagcctcg aaagatattt tttcaaaaag attccttgtg gtatggatga
     2461 ttctattctg taatatcagt gcaggaatat ccataatcgg ctttcaatca cctatgttcc
     2521 aggagcttat tataaacagg aatccttcct caactgcgga agctctcgcc tcgatgggag
     2581 cgactcttat tgctgtaact tctatcttca atggtgtagg aaggttgcta tgggggattt
     2641 tctcggacag atttggaaga ataagggctt atcgcctttt gcttgggagt gaaatcctgg
     2701 tttttctctt gctaatattt acaaggagcc catgggtatt tgctatatgc gcttgctgga
     2761 tccttttctg ctatggagga ggatttggaa tgatgcccgc aaccatttcc gagctattcg
     2821 gaccacaaaa aatggctatt atgtatggtg ccgtattaac tgcatggtcc tttggcggag
     2881 taattggtcc tcaaattacg gcttatatca aggatactca accatctcag gccatcaaga
     2941 tttccttcat cgtgggcgcc gtgtttatcg ccatagcctt tctattttcg attttgatta
     3001 ccgataaaaa gaaataggcc accttctcca gaaaaggcgg cctatctagc agggagagga
     3061 ctcgaacctc cgacctccgg gttatgagcc cgacgagctg c
//
LOCUS       DAOOWY010000168         3099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_31513_length_3099_cov_13.739488, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000168 DAOOWY010000000
VERSION     DAOOWY010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31513_length_3099_cov_13.739488"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(687..2078)
                     /locus_tag="PK105_08665"
     CDS             complement(687..2078)
                     /locus_tag="PK105_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU61828.1"
                     /translation="MKMIDHNQIANLPSEMYAHLRDDVLLKISPSYGIIQRNDGSERR
                     TISRQTAEALTLLDGRQKISKIINQYIHNSNCSDLEICKIMIQVAEFITDGFIVLDNR
                     EIISKSKIEGDFTAFYPISMHVELTKACNLNCYYCYRDAKADQTKDRLETDKLLEILL
                     FLSKKGLHAVELTGGEPLMHPDFSRIMKFVGEHFSLIGLLTNGTLITESILNDMIPFR
                     DKIAVSISLDSHIPSVHDERRGLDGAFDKTTKAIQLFARNGFTTRVTMALDEYNLRHI
                     EPTMQLSRRLGATMFSYAPILPFGRAKNHLNFDFWASNTDEILETEKRLLREYKGFLR
                     LLPEDVLEPNDLGGCGAGHKSYTMDPRGWIRPCPTFDETQAIFGCFEHQDSSGIFGGE
                     LSMSFANIQPPNPEICQTCEFTYFCMNCSLRGLLGMKWVGEENCSWMNQPRIQKWKDL
                     VRSHEYKGIQQSV"
     gene            2838..>3099
                     /locus_tag="PK105_08670"
     CDS             2838..>3099
                     /locus_tag="PK105_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOU61829.1"
                     /translation="MVGSYSEIIEKVEYKEAVVMTAEEVSQLVQAGEASRLTEVDVVT
                     TATRAVMSGTYAVLSFPVASPGSFLRARRAWINGIEAHVGPCP"
BASE COUNT          929 a          681 c          615 g          874 t
ORIGIN      
        1 tcctccttct ccggagaaga gatgcagccc agagacatca cagccaccgc cgccaagatg
       61 attataataa taaatttatt caaatacatc acctagtacc atactttgat tgttcgagag
      121 caagatcggt ataaagcagt ttcccgccgc aatgcattct cttctcaaaa cggataatat
      181 ccagaaatag ttatccaata gtgacgatag gacaggtttg aatagtgtag agtcccgaaa
      241 ttatttcgcc taccatccaa acttggacgt ctatttcagg atatgaagct caacaagacg
      301 aaaatccgat acattccgca cgcatataag cacaagagag attgcgcaga agatcaaagt
      361 ctctcagcgc cgagtttaac agatcccaag caatagagaa aaaccggcta ggagcccgtc
      421 tatgcgaaag agttgggcgt cctataaaag cattcaacga aagcaaggcc aagagcgtcg
      481 ggaccactct cgcccactat agcttgggag cgggcagaga gttctttaaa agtcaatact
      541 gctgccaagg atgccctttg aagcctattt gcgactggac gtagattgtt cagattgtgt
      601 gaaaattata tgaatgaatc aatctctaaa tcaggatggg cgcgaaatat tttcaggact
      661 ctacactttt gaagttatac ccatgcttat actgattgtt gtattccttt atattcgtgc
      721 gatcgaacca aatctttcca tttttgtatt cgaggttggt tcatccaaga gcagttttcc
      781 tctccaaccc atttcattcc tagtagacca cgaaggctgc aattcatgca gaagtaggta
      841 aattcacatg tttgacatat ctctggattg ggaggttgta tatttgcaaa actcatagaa
      901 agttcgcctc cgaaaatacc agatgagtct tgatgctcga aacaaccgaa tattgcttgc
      961 gtttcatcaa atgttggaca gggacgtatc caacctcgcg ggtccatagt gtacgattta
     1021 tgaccagcgc cacacccacc cagatcattc ggttcaagaa catcttccgg taaaaggcgt
     1081 aaaaatcctt tgtactctct taatagccgc ttctcggttt caagaatctc atctgtattg
     1141 ctggcccaaa aatcaaaatt cagatgattc ttggctctgc caaacggtaa tattggagca
     1201 tagctaaaca ttgtagcacc taatcttcga gataattgca ttgttggctc aatatgtcgc
     1261 aaattatatt cgtccaaggc catggttacg cgtgttgtaa aaccatttct tgcgaataat
     1321 tgtatggctt tagtagtttt atcgaaagca ccatccaaac cgcgtctttc atcgtggact
     1381 gatggaatat ggctatctaa gcttatgctc actgcgattt tatctctaaa cggaatcatg
     1441 tcgttcaaaa tggattctgt tataagggta ccatttgtca ataatccaat tagcgaaaaa
     1501 tgttctccaa cgaacttcat gatccgtgaa aaatcggggt gcataagtgg ttccccacca
     1561 gttaactcaa cagcatgcaa tccctttttc gacagaaata gaagtatctc gagcaattta
     1621 tccgtctcaa gtcgatcctt cgtttgatca gccttagcat cgcgataaca gtaataacaa
     1681 ttgagattac atgcctttgt aagctcaaca tgcatactga tgggatagaa ggctgtgaag
     1741 tctccttcaa tcttggactt tgaaataatc tctctattat ccaatactat aaaaccatcc
     1801 gttataaatt ctgccacttg tatcataatc ttgcatattt ccaagtcgct gcaattagaa
     1861 ttatgtatat attgattgat gattttacta atcttttgcc tgccatctaa tagggttaat
     1921 gcttctgcag tttgcctgga aatagttctt cgctcggatc catcatttct ttgaataatt
     1981 ccataacttg gtgagatctt gagcaaaaca tcatcccgaa gatgtgcata catctcactt
     2041 ggtaaatttg ctatctgatt atggtcaatc attttcatcc gtccaggcca taaaaaggta
     2101 attactgttt cttagtgtca atgtcatcaa gtatctttag caaagaggcg gaatgcgatc
     2161 cccgagctac tatccatctc ccacatattg aacaaagacc gcaccaatta caattatggg
     2221 gtatccttgc ctttagtagc gatcgtcgat cactgggctt ctgtcttaac ataggctcta
     2281 tttgatcgat caattcctct ggcaaaacca ttttaaaacc atgcttctga agtatttcta
     2341 gcatactctc gtctgtacag tcggctgcaa ctgcagagtt tataagttct ttaaagaact
     2401 tttcgaattc ctcgtcactc atttccatat gcatgttaat tcaccgcttt tataatgcat
     2461 attaatggta aactacttaa aagtattgcc aaaggccaaa ggctagatgg tgtagagtcc
     2521 taaaagcttg gcatttatat aatcatgcac tttatgatgg ccataggcaa ggcagaagaa
     2581 acaaagtctt ataaatgatt ctgccaaagt caaatacatt atcgggtaaa aaaccaataa
     2641 catcagctcc aaaatcatta acgcagagat gaaggttagc atcctaacag caaatcgaat
     2701 ttggagttac tggatgaaaa aaaagccatg agcgccaaag aaattcagcc tctaaaaaca
     2761 actctacagg ataggtttcc atagcgaaaa gcgtttctat caaatagcta caagtagatc
     2821 aatccaggat gggataaatg gtaggatctt attcggaaat aatagaaaag gtggagtaca
     2881 aagaggccgt ggtcatgacc gcagaggagg tctcccagct ggtccaggca ggggaggcct
     2941 cccggctcac agaagtggat gtggtcacca ccgcaacccg ggcggtgatg agcgggacct
     3001 atgcagtcct ctcctttccc gtagccagcc cgggatcatt cctgagagct cgccgggcct
     3061 ggatcaatgg aatagaggcg catgtcgggc cctgcccca
//
LOCUS       DAOOWY010000169         3093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_31594_length_3093_cov_10.901251, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000169 DAOOWY010000000
VERSION     DAOOWY010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31594_length_3093_cov_10.901251"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK105_08675"
     CDS             <1..188
                     /locus_tag="PK105_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61830.1"
                     /translation="LKGFATLRRVATPPAAHITNRIGRNQCTPKIMLVIRIKEFCYRI
                     KRLAVMLDSIRDLVTTP"
     gene            complement(203..1324)
                     /gene="mnmA"
                     /locus_tag="PK105_08680"
     CDS             complement(203..1324)
                     /gene="mnmA"
                     /locus_tag="PK105_08680"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOU61831.1"
                     /translation="MNQDITIAVGLSGGVDSSLAACLLKEQGYKVIGLTMKIWKGAYK
                     IQENLKHSCFGPSEEEDIDACQRLCANLGIPYHVIDLADEYELHVLEYFRKEYLAGRT
                     PNPCIVCNRDLKFGFLIDKARQSGILFDMFATGHYARKSTINGLTYIRVARDVDKDQS
                     YFLYGLDSARLSYIIFPLGEMTKKETRQAAHFYGLEAAEKSESQDFISGGNYAPLFEN
                     EKPMPGDIVDLKGNILGRHRGLPFYTIGQRRGIGISLGAEPLYVLGLDVEQNRVIVGN
                     GKGLFSSGLIAKPFRLHNPQSFGQPIVCRVKIRQNHKPAEATAEVDKTGSARIEFMVP
                     QRAVAPGQSAVLYSEEGLVLGGGIIEKALPDIAIDTTES"
     gene            1454..2581
                     /locus_tag="PK105_08685"
     CDS             1454..2581
                     /locus_tag="PK105_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU61832.1"
                     /translation="MQDVFIGFTSITWKMLVMYGIGGILIWLAIKKQYEPMLLLPIGF
                     GSILVNLPLSIAWEHDGIPGFLRILYEAGIANELFPILIFIAVGAMIDFGPLFRNPLM
                     IFFGAAAQFGIFATMIVATLLGFDLKTAASIGIIGAADGPTAIYVANKFAKDYLAPIS
                     VAAYSYMSLVPIIQPPVIRLLTTKQERSIHMPLHEKQSPQWLKILFPIVVTMTSGIVA
                     PISVPLVGSLMFGNLIRESGVVERLSQVAQNELAYLVTLLLGICIGGSMSAEKFLNVD
                     TLLILAMGLVAFIFDTAGGVLFAKLVNLFLSKEKKINPMIGACGISAFPMSARVIQKM
                     ANDEERGNIILMQAIGANVSGQLGSIIAGGLVLALVPLIIG"
     gene            2592..2738
                     /locus_tag="PK105_08690"
     CDS             2592..2738
                     /locus_tag="PK105_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61833.1"
                     /translation="MEFIDNIRNAGTTIQAVAVSIGGLLGVFATLAFFFLMILLADRF
                     SKNR"
     gene            complement(2810..>3093)
                     /locus_tag="PK105_08695"
     CDS             complement(2810..>3093)
                     /locus_tag="PK105_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOU61834.1"
                     /translation="GILIAMPTYEYKMFPPMAYVLDIFERKHIYKRKSLRIGSYGWVG
                     GAKKEFEAKLESLKWSCIEAVEWPGAPDEATGELLKEKGRELARWVKAN"
BASE COUNT          760 a          669 c          696 g          968 t
ORIGIN      
        1 cacttaaagg ttttgccacg ttaaggagag tggctactcc acctgcagcg cacatcacaa
       61 accgcatagg tcgaaaccag tgcaccccca aaattatgct tgttataaga ataaaggaat
      121 tctgttatag aatcaagcgc cttgccgtaa tgcttgattc tattcgagat ttggtaacaa
      181 ctccttgaga gttttgaggt cttcagcttt cagttgtatc gatggcaata tcgggtaatg
      241 ccttttcaat gatgccgccg ccaagcacca ggccttcctc ggaataaagc accgcagatt
      301 gaccaggcgc aaccgctcgt tgaggaacca taaattctat gcgcgcactt cctgtcttat
      361 cgacctccgc ggtagcttct gcgggtttat gattttgcct gatttttact ctgcagacga
      421 tgggctgtcc gaaagactgt ggattgtgca gtctaaaggg tttcgcgatc agtccggatg
      481 aaaaaagccc ctttccattt ccgacaataa cgcgattttg ttctacatcc agaccaagca
      541 cgtaaagggg ttctgcaccc aaagaaattc caatgccacg tctctggcca atggtgtaga
      601 atggtaatcc tcgatgtcga ccaagtatgt tgcctttcaa atccacaatg tcgccaggca
      661 ttggtttttc gttttcaaaa agaggagcat aattccctcc gctaataaaa tcttggcttt
      721 cggatttttc tgctgcttct aatccgtaga aatgcgccgc ttgacgtgtt tccttctttg
      781 tcatctcgcc gagtgggaat ataatgtagg ataagcgcgc ggaatccaga ccatatagga
      841 aatagctctg atctttatcg acatctcgag caactcttat atatgtcagg ccattgattg
      901 tgctctttct tgcgtagtgt cctgtggcaa acatatcgaa tagtatgcca gattgtcttg
      961 ctttatcgat caagaatcca aactttagat cgcgattgca gactatacaa ggattgggcg
     1021 ttctgcctgc aaggtattct ttacgaaagt attctagcac gtgtagttca tactcgtcgg
     1081 cgagatctat tacatggtaa gggatgccca agtttgcgca caaacgctgg catgcatcga
     1141 tatcctcttc ttcgctgggg ccaaaacacg aatgcttgag attttcttgt attttatatg
     1201 cacctttcca tattttcatg gtaaggccaa ttaccttgta tccttgttct tttaacaggc
     1261 atgcggccag agaggagtcc actccacccg aaagaccgac tgcgatagtt atatcctgat
     1321 tcatagcgag cgtactttac taggaatagg tttttttttc cagcatcacc atttgtagct
     1381 aagattcaaa tgtgtgcatt tttcttggag tttatacttg ctttgttgtc catatgcagg
     1441 gtaaagtacc tgcatgcagg atgttttcat aggttttact tccattacat ggaaaatgct
     1501 ggtgatgtat ggcataggtg gtattctcat ctggcttgca attaagaagc agtatgagcc
     1561 aatgcttctg cttcctattg gttttgggtc tatacttgta aatttgccac tttcgattgc
     1621 atgggagcat gatggaatac ctggatttct tcggattctt tacgaggcag gaattgcgaa
     1681 tgaattattt cctattctca tctttatcgc tgttggagcg atgattgatt ttggtcctct
     1741 ttttagaaat cctctcatga tattctttgg agcggctgcg cagtttggaa tatttgcgac
     1801 aatgatagtt gctacgctcc tgggtttcga tctaaagact gcagcctcta ttggtattat
     1861 aggtgcggcc gatgggccga ccgccatcta tgtggccaat aaattcgcaa aggactatct
     1921 agcgcctata tcggtagctg catattcata tatgtctctg gtgcctatta tccagccgcc
     1981 ggtaataagg cttttgacaa caaagcagga acgatctata catatgccac tgcatgaaaa
     2041 gcaatcaccc caatggctca agattctttt tccaattgta gtgaccatga cctcaggtat
     2101 cgttgctccg atctccgtac cacttgtagg ttcgcttatg tttgggaatt tgatccgaga
     2161 gtcgggtgtg gtagaacgac tttctcaggt cgcgcagaat gagctagctt acctggtgac
     2221 gctgctatta ggcatttgta tcggaggctc catgtcggct gaaaaattcc tgaatgttga
     2281 tactcttctc atcttggcga tggggcttgt tgcttttata ttcgatactg caggcggagt
     2341 gctttttgca aaactcgtaa atctgttcct ttcgaaagag aagaaaatca atccaatgat
     2401 aggggcttgt ggcatttcgg cttttccaat gtcagcacga gtaattcaaa agatggctaa
     2461 tgatgaagaa agaggaaaca taatccttat gcaggctata ggcgcaaatg tatcgggtca
     2521 acttggttcg atcatagctg gtgggttggt acttgcgctt gtgcctctaa tcatagggta
     2581 ggagttatag aatggaattt attgacaata ttcgaaatgc aggtactaca atacaggcag
     2641 ttgccgtttc tatcggaggg cttcttggtg tttttgctac acttgcattt ttctttttga
     2701 tgatcttgtt ggcggatcgc ttcagcaaga atcgatgaag acaaaagata gcagggcaat
     2761 tcttctaaaa agtcgatttc ttagatgcct cttcggcttg gggtgactat cagttggctt
     2821 ttacccatcg agcaagctcc cggcctttct cttttaaaag ctcacctgtg gcttcatctg
     2881 gagctcctgg ccattctact gcttcgatac aactccactt gagggattca agcttcgctt
     2941 cgaattcctt tttagctcct cctacccaac catagcttcc gatgcgaagt gatttgcgct
     3001 tgtagatgtg tttgcgctca aagatatcga gtacataagc cattggtggg aacatcttgt
     3061 attcataggt aggcattgca atcaatatac ctt
//
LOCUS       DAOOWY010000170         3090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_31628_length_3090_cov_4.671829, whole genome shotgun sequence.
ACCESSION   DAOOWY010000170 DAOOWY010000000
VERSION     DAOOWY010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31628_length_3090_cov_4.671829"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK105_08700"
     CDS             <1..416
                     /locus_tag="PK105_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU61835.1"
                     /translation="APYHVYVVLGDGEIQEGMVWEATMSAAHYKLDNLIFILDHNGLQ
                     IDGTNDQVMSLGDIIGKFHSFGFECKKVDGHNVDMLVKAISEPVPGKPLFVLAETVKG
                     RGISFMENNVGWHGKPMKTEEYTQAMCELKGTQNV"
     gene            409..1341
                     /locus_tag="PK105_08705"
     CDS             409..1341
                     /locus_tag="PK105_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOU61836.1"
                     /translation="MFESKSLRVAYGEALAELGSIDQKVVVLDADLSYATMTSIFASK
                     FPDRFFNFGLAEANMMGAAAGFAHSGLLPFVSTFALFGVGRAYEQIRNSIAYPNANVK
                     FGLSHSGLSVGEDGASHQSIEDIALMRVLPGMTILVPCDPFETRKAVFAAASLDGPVY
                     IRVARPIVEVITNEDSTFTIGKANIMRKPGDVCIVATGLMVKEALAAAERLSLHGIKA
                     GVINMHTIKPIDAELILEMASQCRAMVTAEEHSIIGGLGSAVAEVLVGKSNIKFARVG
                     IQDKFGRSGKPEDLFKAYGLTADDIAQQCIMLLE"
     gene            1371..1796
                     /locus_tag="PK105_08710"
     CDS             1371..1796
                     /locus_tag="PK105_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61837.1"
                     /translation="MSDLIEYEHEGKGLQRVYENKEWMVGIKNYKPENDISNIDCLER
                     HNETDELFVLLRGTCILLYALEDLINDSLELKAISMELDKVYNIPKGLWHNTITQQET
                     KLILIEAASTSQQNSNVIPLSYDQIARAKELISALQKKL"
     gene            1793..2920
                     /locus_tag="PK105_08715"
     CDS             1793..2920
                     /locus_tag="PK105_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU61838.1"
                     /translation="MKKLRVAIIGIGKIGISHLKAIKAIEPLGYAELAAICSLNPNKI
                     KSLCDDYDIPVWYTSYQEMLKSESLDLVHNCTPNVLHYDVNRGILEAGLYDLSEKPLT
                     IDSQQSGALVALAKNRHLKTAVNFVYRYYPIIMHIKELISKGELGTIYSIHGTYLQDW
                     LLFDHDYDWRVESRYGGPSRALADIGSHWIDIAEFLLDRRVESIAADLATFIPKRIRS
                     PSETIIVDTEDFASVLMRFEGGIHGCLTVSQVSAGRSNGISFEIDGSIASLRWSQEHP
                     ERASLKYREEEELFIDEKGTISLANELEENKEANAGASKQERFLEAQQRMIDNFYRSI
                     VFDDVPIYADFEQGHRIVKIIEAAIESNQKERWIHSRHEGS"
BASE COUNT          907 a          610 c          726 g          847 t
ORIGIN      
        1 gtgcaccata ccatgtctac gtggtgcttg gcgatggaga aattcaagag ggcatggtgt
       61 gggaggcaac tatgtctgca gctcattata agctcgataa tttgatcttt atacttgatc
      121 ataatggctt gcaaatagat ggaaccaacg atcaggttat gtccttgggc gatataattg
      181 gaaaattcca ctcctttggt tttgagtgca aaaaagtcga tggacataac gtcgacatgc
      241 tggtaaaggc gatttccgaa ccggtgcctg gaaagccctt attcgtgctc gcagagacag
      301 tcaagggcag gggaatctca ttcatggaga ataatgttgg ctggcatggc aaaccgatga
      361 agactgagga atacactcaa gcaatgtgcg agctgaaagg aacgcaaaat gtttgagagc
      421 aaatcattga gagtcgccta tggcgaggcg cttgctgagc ttggttcaat cgatcaaaag
      481 gtggtggtgc tggatgcgga tctttcttat gcaacaatga cgagtatttt tgcatctaag
      541 tttcccgatc ggtttttcaa ctttggtctt gctgaagcga atatgatggg ggcagctgcg
      601 gggtttgccc attcagggct tttgcctttt gtaagtactt tcgcgctctt tggtgtagga
      661 agagcatatg agcaaattcg taattccatc gcatatccca atgcaaatgt gaaatttggg
      721 ctctctcact caggactttc agtaggcgaa gatggcgcaa gtcatcagag catcgaagat
      781 atcgcactca tgcgagtact accagggatg acaatccttg ttccttgtga tccttttgaa
      841 actcgcaaag cggttttcgc tgccgcaagc ttggatggac cggtatatat tcgcgttgct
      901 cggccaattg tcgaggtaat aacaaatgaa gacagtactt ttacaatcgg caaggcaaat
      961 attatgcgta agcctggtga tgtctgcatt gtggctacag ggctcatggt gaaagaagca
     1021 ctcgcagctg ctgaaagatt atctcttcat ggcataaaag ctggcgtcat caatatgcac
     1081 accataaagc caatcgatgc tgagttgatt ttagaaatgg cttctcaatg tcgagccatg
     1141 gtgaccgctg aggagcattc gatcatagga gggctagggt cagctgtcgc tgaagttctt
     1201 gtaggcaaat caaatataaa gtttgcccgt gttggtatcc aggataaatt tggacgttct
     1261 ggaaaacctg aagatctttt caaggcctat ggtcttacag cagatgatat cgcgcaacaa
     1321 tgcatcatgt tgttggagta aaaaggaagt aattcaagag gtaattattt atgagtgatt
     1381 taatagaata tgaacatgaa gggaaaggcc tccaaagagt atatgaaaac aaggaatgga
     1441 tggttggaat caagaattat aaaccagaga atgatataag taatatcgat tgtttggaac
     1501 gtcacaatga gaccgacgag ctctttgtct tattgcgcgg tacttgtatc ctgctatacg
     1561 cgctagagga cctaattaat gatagcctcg aactgaaggc tatctccatg gagctagaca
     1621 aagtatacaa tattcctaag gggctttggc acaatactat tacgcaacag gaaaccaaac
     1681 tcatccttat tgaagctgca tctacatcgc aacagaacag caatgtaata ccattatcct
     1741 acgatcagat tgctagagca aaagagctta tatcagcgct ccagaaaaag ctatgaaaaa
     1801 acttcgagtg gcgattatcg gaattggcaa gataggcata tctcatctca aggctatcaa
     1861 agctatcgag cctctcggct acgcagaatt ggcagcgatt tgttctctca atccaaacaa
     1921 gataaagagc ctctgcgacg actacgatat ccctgtttgg tatacttcat accaagaaat
     1981 gcttaaaagc gagagtctcg atctcgtgca taactgtact cccaatgtgc ttcactatga
     2041 tgtaaataga ggtattctcg aagcaggctt atatgatcta tctgaaaagc cgctcaccat
     2101 agattcacag caatcgggag cgcttgtggc tttagcaaag aatcgtcatc taaaaaccgc
     2161 tgtcaatttt gtttatcgat attatccaat cattatgcat ataaaagaat tgatatccaa
     2221 gggcgagctt ggaacaatct attccataca cgggacttat cttcaagatt ggctcttatt
     2281 cgaccatgat tacgactggc gggtcgaatc tcgctatgga ggaccatcga gagcccttgc
     2341 tgatattgga tctcattgga tagatatagc agagttcttg cttgatcgca gagtggaaag
     2401 catcgccgca gaccttgcaa cattcattcc caagcggatt cgctctccct ctgagactat
     2461 aatagtagat actgaggatt ttgcaagtgt cctgatgaga tttgaaggtg gcattcatgg
     2521 ctgcctaact gtctctcagg taagtgctgg tcggtctaat ggcatctctt ttgagataga
     2581 tggtagcata gcatctctgc gttggtctca agagcatcca gaaagagctt cgcttaaata
     2641 tcgagaagag gaagaattat ttatagatga aaaaggaact atatcgcttg cgaatgagtt
     2701 ggaagaaaac aaggaagcaa acgctggtgc ttcaaaacaa gagagatttt tggaagcgca
     2761 acagaggatg atcgataact tctatcgctc aattgtgttt gatgatgttc ctatctatgc
     2821 tgattttgaa caaggccata gaattgtaaa aatcatagaa gctgcaattg agagcaatca
     2881 gaaagaaaga tggattcata gccgccatga aggctcctga cttttttagt taaggataac
     2941 tttagccttc atcaacattt ctatttttcc tgcctggata ttattttctc caatgcttac
     3001 tctctataag cctccgaaaa cctatgcgat aatccaataa gttgctcgac ttcgaggcgc
     3061 atcgattcgg tgaagagccg tgcatccgcg
//
LOCUS       DAOOWY010000171         3058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_32037_length_3058_cov_9.428904, whole genome shotgun sequence.
ACCESSION   DAOOWY010000171 DAOOWY010000000
VERSION     DAOOWY010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32037_length_3058_cov_9.428904"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..586)
                     /locus_tag="PK105_08720"
     CDS             complement(104..586)
                     /locus_tag="PK105_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU61839.1"
                     /translation="MKRMLGTILVAINGSDASICAFKYALALAKAFGSKIIACYVVDT
                     ATIRQLALSRIFVPEESEEYERNLENSGLRYLNFCVELAHQKQMYIETSIRRGSVSGE
                     IVKYSMEIDADIIVLGGNPVDVLYRDAIADSNHEVLKNARCPVLFVKPPIGDDIYKSI
                     "
     gene            complement(583..1215)
                     /locus_tag="PK105_08725"
     CDS             complement(583..1215)
                     /locus_tag="PK105_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOU61840.1"
                     /translation="MAEQMPAPTKTNLIKEKRNLQLALQGYDLLEKKREILVIELMKR
                     MDSLELLEREVADITEKAYAALRKMLLLVGRERALAISSMPVREITLSASRINVSGMR
                     LPSLDVHASSPTLNYSFMNSFAICDETVIEFTELLDKLSTAAGMRSIVWRLAREVRKT
                     QRRVNALDKMVIPRSQSIVKYIDSSLDERERESLFAVKMLKKRQIGQQES"
     gene            complement(1219..2673)
                     /locus_tag="PK105_08730"
     CDS             complement(1219..2673)
                     /locus_tag="PK105_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOU61841.1"
                     /translation="MRGLEYHGIERADGPIIIAERREDIGFSELVRVKDRQGEYRLGR
                     VVELSDKAVAIQLFAENTGISIDDAWVEYLEKPLTFRVGEGIVGRIFNGLGEPIDGYP
                     PILSSDLRDINGLPINPSARVYPRDFIQTGISAIDGMNTLIRGQKLPIFSGNGLPHNR
                     LAAQIVRQAKVLSGESQFAIVFAGMGIKYDVARFFIDSFESSGVLGRVVMFLSLADSP
                     SIERLVTPRTALTAAEYLAYEKNMHVLVVMTDMTNYCEALREVSAARNEVPSRKGYPG
                     YLYSDLASIYERAGRIEGSEGSITQLPILSMPNDDISHPIPDLTGYITEGQIVLEREL
                     SQRGVYPPIAGLPSLSRLMKDGIGEGMTREDHRELAAQLFSAYAYVHTVRDLAAIIGE
                     EELSDRDKIFLNFGDHFESQFLAQGEFENRSIEQTLDLGWKMISLLPESELVRIPPQI
                     LAKYLPRDDRDDQNDRGGHKESHEVAALKEPKEK"
     gene            complement(2670..>3058)
                     /locus_tag="PK105_08735"
     CDS             complement(2670..>3058)
                     /locus_tag="PK105_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOU61842.1"
                     /translation="KERRLSDIVRLIGPDALPDEQRLILLTADMIRNGFLQQSSFDRV
                     DMYCAPQKQTLLLQCILTFHELAETAIKNGAPLPKVSALPIREKIVRLKSSLENDKVE
                     EGRMVIQEIQVAFEQLGVTVQGAVLA"
BASE COUNT          784 a          785 c          679 g          810 t
ORIGIN      
        1 gatcacagaa agaattgatt atttcaaggc aattagaaat tactggatca atcatacgga
       61 ttcttgaaaa caagcaaacc aaagcctatc attcaattca atactatata gacttgtata
      121 tatcatcccc tatgggcggc ttgacaaaga gcacagggca tcgggcattt tttaggactt
      181 cgtgattgga atctgcaatc gcatcgcgat agagcacatc gactgggtta ccgcctagca
      241 ctatgatatc tgcatcgatc tccatcgaat acttgactat ttctccagaa accgagcctc
      301 tgcgaatgga agtctcaata tacatttgtt tctgatgagc aagttcgaca caaaaattta
      361 gatagcgcag gcccgaattt tcgagatttc gctcgtattc ttcactctct tcgggtacga
      421 agattctgga gagcgcaagt tgacggatag tagccgtgtc gaccacatag caggcaataa
      481 tcttcgaacc aaaggccttt gcgagcgcca atgcatattt gaaagcacat atggaggcat
      541 ctgaaccatt gatcgccacc aaaatggtcc ccagcattct cttcatgatt cttgctgtcc
      601 tatttgccgc tttttaagca ttttgactgc aaacaacgat tcgcgttccc gctcgtccag
      661 agaagaatcg atatatttta caatcgattg tgacctaggt atcaccatct tgtccaatgc
      721 gttcactctg cgttgagtct tgcggacttc ccgcgcaagg cgccatacaa tcgatctcat
      781 tcctgccgct gtcgagagct tatcaagaag ctcagtaaac tctatcacag tttcgtcaca
      841 tatagcaaat gaattcataa aagaataatt caaagtgggg ctactggcgt gtacatcgag
      901 cgaaggaagt cgcatcccgc tgacattgat acgtgatgcg ctgagagtaa tttcgcgcac
      961 cggcatagat gaaattgcaa gagccctctc tcgaccaacc aaaaggagca tcttccgtaa
     1021 ggctgcatag gctttttctg ttatatccgc aacttccctt tccaggagct cgagactatc
     1081 catgcgtttc attagctcga tgacaagaat ttccctcttt ttctcgagaa gatcatagcc
     1141 ctgaagcgca agttgcagat ttcgcttctc cttgatgaga ttcgtcttgg tcggcgcagg
     1201 catttgctca gccatgggct atttctcctt tggctctttt agggcagcca cttcatggga
     1261 ttctttatga ccaccccgat cattctgatc atccctatca tcacgcggca gatattttgc
     1321 aagaatctgg ggcggaatac gaacaagctc agattccggg aggagcgata tcatcttcca
     1381 acctaagtcg agcgtctgct ctatactgcg gttctcgaac tcgccttgcg ctaaaaactg
     1441 gctctcaaaa tgatcaccaa aattcaggaa tattttatcc ctgtccgaaa gctcctcttc
     1501 tcctatgatt gcggctagat ctcgcaccgt gtgaacatag gcataggcgg aaaaaagctg
     1561 ggcagcaagt tctcggtgat cctctcgagt cataccctcc cctatgccgt ctttcatcag
     1621 ccgcgaaagg ctaggaagcc ccgcgattgg cggatacacg ccacgctgcg aaagttcccg
     1681 ctctagaacg atctgacctt cggttatata acccgtaagg tccggaatgg ggtgtgatat
     1741 atcgtcgttg ggcatcgaga gaataggaag ctgagttatg gaaccttcgc tgccttcgat
     1801 tcttcccgct ctctcgtaga tcgaagcgag gtctgagtat agatagcctg gataaccctt
     1861 tcttgaaggc acctcgttgc gggctgcgga gacttcacga agagcctcac aatagtttgt
     1921 catatcggtc atgaccacta gaacatgcat gttcttctcg tatgcgagat attcagcggc
     1981 ggtcagcgct gtgcgcggcg ttacaaggcg ttctatcgat ggactgtccg cgagcgaaag
     2041 aaacattaca actcggccta agacgccaga gctctcaaaa gaatctataa agaatcgggc
     2101 aacatcgtat tttattccca taccggcaaa tactattgca aattgacttt ctccgctgag
     2161 tacttttgcc tgcctcacaa tctgggctgc gagcctatta tgaggaaggc cattgccgga
     2221 aaaaatcggc aacttttggc cacggataag ggtattcatg ccatctattg ctgaaattcc
     2281 ggtctggatg aagtccctgg ggtacacgcg agcgctcgga tttatgggca ggccgtttat
     2341 atccctgagg tctgaagaca atataggcgg atagccatct atgggctccc caagaccatt
     2401 gaagattctg ccaacaatgc cttcgccaac acggaaggtc aagggctttt cgaggtattc
     2461 gacccaggca tcgtctatcg atatgccggt attctctgca aagagctgaa tggccactgc
     2521 cttatcagat agctccacaa cccggccaag tcgatattct ccctgcctat ctttgaccct
     2581 aacgagctcg gaaaaaccga tgtcctctct gcgttcagca atgataatcg gcccatctgc
     2641 gcgctctatt ccgtggtatt caagacctct catgctagaa ccgctccttg taccgtaaca
     2701 ccaagctgct caaaggcaac ctgaatttcc tgtattacca tcctgccttc ttctacctta
     2761 tcattctcta gcgaactctt gaggcgaaca atcttctccc gtattggcag cgcggatacc
     2821 ttgggaagag gagcaccatt ctttattgca gtctccgcaa gctcatggaa agtcagaatg
     2881 cattgaagca aaagagtctg cttctgagga gcgcaataca tatcgactct atcaaaactg
     2941 ctctgctgaa ggaaaccgtt tcttatcata tctgcagtaa gcagtatgag tcgctgctca
     3001 tctggcagcg catctgggcc aatgaggcgc acgatatccg aaagccttcg ctcttttt
//
LOCUS       DAOOWY010000172         3040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_32316_length_3040_cov_28.359799, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000172 DAOOWY010000000
VERSION     DAOOWY010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32316_length_3040_cov_28.359799"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="PK105_08740"
     CDS             complement(<1..1357)
                     /locus_tag="PK105_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61843.1"
                     /translation="MKRSLFIQGNMAKAGAAKSFTRAFAVLVLISMIFVISPLAEMSL
                     LAAQTLGGSSISTAVQSGMTSGAAQGSSAGQSGGALLPEATARAQIAMATADYPVTPG
                     DVYSLIYMRASQTDSLRIVVEGDGKANLGFLGKLSSEGMTFRAFKALVESKIEAAYPQ
                     STPSLLIVSTGVFPIKVQGHVQETSIVSAWGLTRLSEVVAGKLLAYSSLRDVQVASSN
                     GKVAVYDLFKAEREGDLGQDPYMRPGDVVELRKADRVVRLEGEVRRSGSYQLKAGEGV
                     GELVGYYGDGLGVSAKSDMVVLTRSASEGKPESESVVFDLSSEELPALLDGDSVRVPS
                     REEYLPIVYVEGAVKPEVMVGQEEKAQEGEEEAKETYGLVRVVYREGQLLSQVVEPLK
                     ERFSEQGDLEHAYIVRGSERIGVDLERLLYQYSKEKDILLMAGDRVVIPYGYASVYVK
                     GE"
     gene            complement(1434..2708)
                     /locus_tag="PK105_08745"
     CDS             complement(1434..2708)
                     /locus_tag="PK105_08745"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOU61844.1"
                     /translation="MSDQKLEMEGQVAQSSQEKNRDNDEISLLDLVAVLWKRKWLVIA
                     ITGLVAFGSLAYSIGSLLMPPDKSYLPNVYTPKATMLISSGTTSSLSSLLSSSGLSGL
                     AGMAGVSAGGNANQQLAKVLATSNTTLDRLNERFNFAEHYKMKEPKVAEVRKAISNNL
                     SATIDEKSNIFTISFTDKDPAFAKEVVDETVNILSERFAQLSGSQAIQQKALLEKRLA
                     DVDAAISTLEAQVKEFQKKYGVVQVEALATEQITILARLRSELILKEMEIENYKKVAR
                     INDPMLTQLNNERDALLAKIKEIETGSGSGSKVMPSQQQLPTIAFEYAKLERDLAVQA
                     ELFKILTQQYEVAKFNAAGQEPVFQILEMAEVPDMKSGPSRSMICIVATLAAFFVSIL
                     LAFASESISKIRRDPEAVARFRAISKGEKPGI"
BASE COUNT          711 a          846 c          686 g          797 t
ORIGIN      
        1 cttcaccctt cacatatacc gacgcatacc catagggtat cactacacga tccccagcca
       61 tgagtaggat atccttctct ttactgtact ggtacaacag cctctcaaga tctaccccta
      121 tccgctcact acccctcact atatatgcat gctctaaatc cccttgctca gaaaacctct
      181 cctttaaggg ctcaactacc tgcgatagta actgcccttc cctatatact acccttacta
      241 atccatatgt ctcctttgct tcttcctcac cctcttgagc cttctcctct tgccctacca
      301 taacttctgg ctttacggct ccttctacat acactatggg caaatactct tccctactcg
      361 gaactcgcac actatcccca tccaagagcg ccggaagctc ttcactgctt aagtcaaaga
      421 ccacactctc actctctggc ttcccttcac tagcactcct agtcaacact accatgtcgc
      481 tctttgcact cacccctagg ccatcgccat agtacccaac tagctcaccc accccctctc
      541 ctgctttaag ctgatagctc ccagacctcc tcacctcgcc ctcaagcctc actacccgat
      601 cagccttcct cagctctacc acatccccag gacgcatgta cggatcctga cctaaatccc
      661 cctctcgttc cgccttgaag agatcgtaaa ccgcaacttt gccattcgat gaggcaacct
      721 gcacatcgcg aagcgaagaa taggcaagaa gcttgccagc aactacctca gagagccttg
      781 tgagacccca tgcacttaca atggaagtct cctggacatg cccttgaacc ttaatgggaa
      841 aaacgcccgt ggagacaatc aaaaggcttg gtgtggattg aggatatgct gcttcgattt
      901 tactctccac aagcgctttg aatgcccgga aagtcatgcc ttctgaagaa agcttgccaa
      961 gaaatccgag attggctttt ccatctcctt ccacaacgat tctgagcgaa tcagtctgag
     1021 aagcgcgcat atagataagc gaatacacat cgcccggcgt taccggatag tccgctgtcg
     1081 ccatggcgat ttgagctcgc gcggtcgctt cgggaagcaa agcacccccc gactgcccag
     1141 cactggatcc ctgggcagcg ccagaagtca tgcccgactg cactgctgtt gaaatactcg
     1201 agccgccgag tgtttgagct gcgagcaacg acatctctgc aagcggagat atgacgaaaa
     1261 tcattgagat cagaaccaga acagcgaatg cgcgggtaaa gctctttgcc gcgcctgctt
     1321 ttgccatgtt tccctgaatg aaaagagatc gtttcatttc tcgtcctttt gccttcttgc
     1381 tttcatagaa agaatatcac gctcatagga atagcccctt gattgcccct ctgctatatc
     1441 ccaggcttct cgcccttgct tattgctctg aatcttgcta ctgcttctgg gtcgcggcgg
     1501 atcttggaaa tcgattctga ggcaaaggca aggagaatcg acacgaagaa tgctgcaagg
     1561 gtcgccacaa tgcagatcat gctgcgcgaa gggcctgact tcatgtctgg tacttccgcc
     1621 atctcaagta tctgaaaaac aggctcctgg cctgcagcat tgaacttagc gacttcgtac
     1681 tgctgggtga gtatcttgaa gagctcagcc tgtactgcaa gatcgcgctc gagcttggca
     1741 tattcaaaag caatagtggg cagctgctgc tgactgggca taaccttcga gcccgaaccg
     1801 gagcctgttt cgatttcctt gatcttagca aggagcgcgt cgcgttcgtt gttgagctga
     1861 gtgagcatgg gatcgttgat ccgcgcgact ttcttgtaat tttcgatctc catctccttt
     1921 aagatgagct ccgaccgaag gcgcgcgaga attgttattt gctctgtggc gagagcctcg
     1981 acctggacga cgccgtattt cttctggaat tctttgacct gagcctcgag agtgctgata
     2041 gcggcatcga catccgcaag cctcttttcc agaagagcct tctgctgaat tgcctggcta
     2101 ccgctcagct gcgcaaaacg ctcggagaga atattgacgg tctcatcgac gacctctttg
     2161 gcaaaggccg gatccttatc ggtaaatgag atcgtgaaga tattcgactt ttcatcaatg
     2221 gtggccgaca gattattgga gatagctttt cttacctctg ctacctttgg ctccttcatc
     2281 ttgtaatgct cggcaaaatt gaacctttca ttgagcctat cgagcgttgt gttggaagta
     2341 gccagtactt ttgcaagctg ctgattcgca ttgcctcctg ctgagactcc cgccatgcct
     2401 gcaaggcccg agagccccga cgatgataac aatgacgaaa ggctgcttgt ggtccccgat
     2461 gaaataagca tcgtcgcctt gggtgtgtag acattcggca gatagctctt gtcgggaggc
     2521 ataagcagcg aacctatcga ataagcgagc gaaccaaagg ctacgaggcc ggttatggca
     2581 attacgagcc acttgcgctt ccagagcacc gcgacaagat cgagcaggct aatctcatcg
     2641 ttgtctctgt ttttctcttg gctactctgt gcgacctgac cttccatttc taatttctga
     2701 tctgacattc cttcctctct gggtttctga ctatattata tcatcgacgc atattcgtgc
     2761 agtcctgttt aacaagaaag cttcgacagg atattcagaa ttttgcatga tgtagggggt
     2821 tttttgaccg gatgagctgg ataatttttt agcaggatga aataggatgt acgggatagt
     2881 ctcttgatag gatgggcagg atgggaaagg atgaacggta tttttcataa gacttcttgt
     2941 agctctttag gcacgggcgg caggtccctg aaccatttgt ggtacagcct gctgtggggc
     3001 gcaaaaagca cgatgatggt gaggatgatg attttgaagt
//
LOCUS       DAOOWY010000173         2971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_33277_length_2971_cov_13.822702, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000173 DAOOWY010000000
VERSION     DAOOWY010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33277_length_2971_cov_13.822702"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1211)
                     /gene="pgsW"
                     /locus_tag="PK105_08750"
     CDS             complement(6..1211)
                     /gene="pgsW"
                     /locus_tag="PK105_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOU61845.1"
                     /translation="MKSSFAKHRQNRAISIRVFIAVISLALGLVCTELLARNEPHPVA
                     KEMKMAAQRMAQAEAVVAKRRLAMGLPIDPALDPYQTGLIGVESSILTTTVGDIVSKR
                     TSTNTAFAALIVRYFYDIGLEPGDRIAVGSSGSFPAILLAVLSACAETRVEPIVITSI
                     GASEYGANIEGFSNAEIMAACRDAGVLPYMPIAISPGGEDDLGISSMYRFEPSDELAR
                     YAHSVAQSLAIPLILASDLEGSFKAHLEAYEKASPIRLFVNIGGADVNFGSGPASLKL
                     KPGLIIPSARTRKTDLGKLHKQAPKDPEKSAQQVTSRSGTMDADSGVDAQAGLIAYYL
                     AKGIPVLHFLNIKGLAVQSGIPVDGDPRAPLPSSIVSTRKKPSWPLIVSLLIAGTALA
                     IRKPLRYYP"
     gene            complement(1220..1651)
                     /gene="pgsC"
                     /locus_tag="PK105_08755"
     CDS             complement(1220..1651)
                     /gene="pgsC"
                     /locus_tag="PK105_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOU61846.1"
                     /translation="MTSISIIVSIVLGFFFGEMTGWLTGGLVAPGYLALYIDQPLRIL
                     MTYMAALLAFFLIKLLAKITILFGRRRFMAFILAGICMGALLDAVVRLIPPSGTDLRA
                     IGYVVPGLIANDIWKQGLWRTLLGSLVITLATRVVLLLILA"
     gene            complement(1648..>2971)
                     /locus_tag="PK105_08760"
     CDS             complement(1648..>2971)
                     /locus_tag="PK105_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61847.1"
                     /translation="ITNVRLDHLDTMGTELESIAEALGESIPDRGKAFVGSEGMGEYI
                     QAVFSRMAAQRGSELIFVPSAEETQALHRDFLYPVHAQNMALALAVCDACGVSRQIAL
                     RGMQSAKPDPGVRSTILTSWRVHKVRVINAFAANDPQSTLEMWHRERGLRYSSDLCYP
                     SDMRNLDELDSGYGLQKNETHEILSEIKEVNSAEQTFRVLVFNHREDRAWRALQIGEI
                     AAGINADAILVYGMNQRLAEKVIYRFYRRLGVGKLTEPKTQQAGLVPELAQSELTTAA
                     LHFASAVSHLQLLPWLQRVSPASPQAQLEPQSAQSQTQIPSKPIKYPIVRSMRQASFD
                     ALCETIESLGGFKESPLKTYVEILCAGNIKGPGMDLSDSLEALLLHRPDVARSAISTE
                     GAQSISSPEGEQSFDSTGVAQPVTSAEGAHSVVSPEKSQSANSTGEKR"
BASE COUNT          766 a          765 c          701 g          739 t
ORIGIN      
        1 tattgctatg gatagtatct aagaggcttt cggatggcaa gtgccgtacc agcaatgaga
       61 agacttacaa taagaggcca actcggcttc ttacgcgtag agacaatact ggagggaaga
      121 ggggccctcg gatcgccatc cacgggtatt ccactttgca ccgcaagacc ctttatattt
      181 aaaaaatgta ggacaggtat acccttagct aaataatagg caattagacc agcttgggca
      241 tctacgccgc tatcagcgtc catggtccct gacctgcttg taacctgttg ggcagatttt
      301 tctgggtctt ttggtgcctg cttatgcaat ttacccaaat ccgttttcct tgttcgagcc
      361 gatggaatga tgagccccgg tttcagcttt agcgaggctg gaccgctacc aaagttgaca
      421 tctgctcccc ctatattgac aaaaagcctt attggcgagg ctttttcata tgcttcgaga
      481 tgggctttga aagagccttc gagatccgaa gctagtataa gagggatagc gaggctttgc
      541 gctacactat gtgcatacct ggctagctcg tcgcttggct caaatctata catggaagat
      601 attcccagat catcctctcc gccaggagag atagcaatag gcatataagg caatactcca
      661 gcatctctac atgctgccat tatctcggca ttgctaaagc cttcgatatt cgctccatat
      721 tcagaagcgc ctatcgaggt gattacgatt ggctctactc ttgtctcagc acaggcagag
      781 agcacagcca aaaggattgc aggaaaagag cccgatgaac ccacggctat cctgtctccc
      841 ggctcaagac caatatcata gaaatatcgc acaataaggg cagcaaaagc cgtatttgtg
      901 ctcgtcctct tagaaactat atcgcctaca gtggttgtca aaatgctcga ttctacgccg
      961 atcaggcctg tttgatatgg gtcgagagca ggatctatgg gtaagcccat agcaaggcgc
     1021 cttttcgcga ctacagcttc tgcctgagcc attcgttggg ctgccatttt catctctttg
     1081 gcaacagggt ggggttcgtt gcgcgcgagc aactctgtgc agacaagacc aagcgcgagc
     1141 gaaatgaccg cgataaaaac gcgaatagaa atcgcgcgat tctgcctgtg ttttgcaaac
     1201 gagcttttca tggaatgact caagccagga tcaagagcag cacaacacgc gttgcaagag
     1261 taattacaag gctgccaagc aaagtcctcc aaagaccctg cttccatata tcatttgcaa
     1321 taaggccagg aaccacatag ccaatcgctc gcagatcggt gcctgaggga ggaattagcc
     1381 ttacaacagc atccagcaaa gcacccatac aaatgccggc tagaatgaag gccataaaac
     1441 gccttcttcc gaataagatg gtgatctttg caagcaactt aatcaaaaag aaggccaaaa
     1501 gagctgccat gtatgtcatg agtattcgca agggctggtc aatataaagt gcgaggtatc
     1561 cgggtgcaac gagtccgcct gtaagccatc ctgtcatttc gccaaagaaa aatccgagca
     1621 ctattgaaac aataatcgat atgcttgtca tcgtttttct cctgtcgaat tagcagattg
     1681 cgatttttca ggcgaaacaa ctgaatgtgc cccctcggct gaggtaactg gttgcgctac
     1741 gccagtcgaa tcaaacgatt gctccccctc aggtgaagaa atcgattgcg ccccttcggt
     1801 cgaaatagcc gatcgtgcaa cgtctggtcg atgcaataat agagcttcga gagaatcact
     1861 caaatccatc cccggtcctt ttatgttacc tgcgcatagt atttccacat aggtcttcaa
     1921 tgggctttcc ttaaaacctc cgaggctttc aattgtctcg catagagcat cgaagcttgc
     1981 ttgtctcatc gagcgaacaa tggggtattt tatgggctta ctggggattt gagtctgcga
     2041 ctgtgctgac tgcggctcaa gctgcgcctg aggtgacgct ggacttactc gctgcaacca
     2101 tggcaataac tgcaaatgcg atacggccga agcaaaatgc agcgcagctg ttgttaactc
     2161 tgactgcgct agctctggta caagcccagc ttgctgagtt ttgggctctg tcaactttcc
     2221 tacgcctagt cgacgataaa atcgatagat aactttttcg gctagccttt gattcatgcc
     2281 atatacaagg attgcatccg catttatgcc cgcagcgatt tcgccaatct gaagggctcg
     2341 ccaagcacga tcttctcgat gattgaaaac aagcacgcgg aaagtctgct ccgcactgtt
     2401 gacctctttt atttccgaga gaatttcatg tgtctcattc ttctgaaggc cataaccgct
     2461 atctaattca tccaagttgc gcatatcgct cgggtagcac aagtcgctcg aataacgcaa
     2521 gccacgttcc ctgtgccaca tctcaagcgt agactgaggg tcgttagccg caaaggcgtt
     2581 gatgactcga accttgtgta ctcgccagct tgtgagaatc gtggatctca cgcctggatc
     2641 tggcttagcg ctttgcatgc cgcgcagcgc aatttggcgc gatacaccgc aagcgtcgca
     2701 caccgcaagc gcaagagcca tgttttgcgc atgcactggg tacaaaaaat ctctgtgcag
     2761 cgcttgcgtt tcttcggcgc tgggcacgaa gataagctca gaaccgcgct gcgcggccat
     2821 acgcgagaaa acagcctgta tatattcgcc cataccttcg ctgcccacaa aggcctttcc
     2881 tctatctgga atagactccc cgagagcctc ggctatagat tcaagctccg tccccattgt
     2941 atccaaatga tcgaggcgca cattagttat g
//
LOCUS       DAOOWY010000174         2896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_34388_length_2896_cov_15.394579, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000174 DAOOWY010000000
VERSION     DAOOWY010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34388_length_2896_cov_15.394579"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PK105_08765"
     CDS             <1..217
                     /locus_tag="PK105_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU61848.1"
                     /translation="AYFWPKIATTTPLRRTIALGVYELKRSYAGLETMNQNQIMAGAV
                     ITIVPIIILFILLQKYILTGFSKVASK"
     gene            214..1320
                     /locus_tag="PK105_08770"
     CDS             214..1320
                     /locus_tag="PK105_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU61849.1"
                     /translation="MIINETKAHPIEALSIDTISNEAFSIKSQKRKPKPYRLRYSNAT
                     FMRCKTAFIFVTLCIVFGIRTVSLTWAQIESAAIVDRYDEVFKRENSEKRLTAWFLKL
                     SLSEESNIDEKSGDSTIIISPDDRVMLIDGGALSCGALVCSYLHALEVDRIDAILLSH
                     PHIDHVGGLISVLQEFPVEMIYMSKLEYSTTTFDSFLSIAADNKVPIIYLQEGSEFDL
                     GSKIHAKVYNPEANIVYYFGYPANSTQFVNDRSLVVKLAFGKSSILFMGDVYTSRELD
                     LIEKYGSELKADVLKVGHHGSDTSSSKSFIRLVCPKTAVIMHDRLASLQVYNNLKKIG
                     SEVYLNCIDGNIRLSIDEEKNLTIITQFDRIVDF"
     gene            1895..2596
                     /locus_tag="PK105_08775"
     CDS             1895..2596
                     /locus_tag="PK105_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOU61850.1"
                     /translation="MRMIEILRDIWGTAKTVLPLAMILVILQVIVLKKPIKNLREFII
                     GILLSIAGLHFFLKGVFMSILPLGDSVGGNIAILDNKLLIVAISLVIGYLGTLVEPAL
                     KTLALEVEEVSVGAIPNKVLIHAVAIGFGLGMGIGVWKIINNISFIKIVIPLIFVIIL
                     LIPFVPKEFVSIAMDSASATTGPVNIPLNMAIAVGLAKSLENVDPLTSGFGIIGLTSI
                     GAVISVMVLGLLTRI"
     gene            2624..>2896
                     /locus_tag="PK105_08780"
     CDS             2624..>2896
                     /locus_tag="PK105_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOU61851.1"
                     /translation="MEVKCIVAIVERGKADAIVEKAKKAGADGATILYGRGTGQPEAL
                     RFFNLHIEASKEVIIILSDTDKYKPIYDAIIEEGNLHEPGTGIIFTF"
BASE COUNT          870 a          591 c          598 g          837 t
ORIGIN      
        1 tgcgtatttc tggcctaaaa tcgccaccac caccccattg cgaaggacca tcgccctggg
       61 cgtctatgaa ctcaagcgct cttatgcagg cctggaaacc atgaatcaaa accaaattat
      121 ggcaggagct gttataacga ttgtcccaat tattatccta ttcattctcc ttcaaaaata
      181 tatccttacc ggattctcta aggtggcttc aaagtgatta tcaatgagac gaaggcacat
      241 cctatagagg ctctttctat agataccatt tctaatgaag ccttttctat aaagtcccaa
      301 aaaagaaaac caaagccata ccgcttaaga tacagcaatg ccacttttat gcgatgcaag
      361 actgcattca tttttgtaac actgtgcatt gtctttggga ttcgaactgt cagccttaca
      421 tgggctcaaa ttgaatctgc tgccattgtg gatcgctatg atgaggtatt taaacgagaa
      481 aatagcgaaa aaaggttgac tgcttggttt ctaaagctat cattatccga agagtcgaat
      541 atcgatgaaa aatctggaga tagtactatt attatctcac cagatgatag agtgatgctt
      601 atagatggcg gagccttatc ctgcggtgcc ttggtatgtt cttatctgca tgctcttgaa
      661 gtcgatagaa tcgatgcaat actactgtcg catcctcata tcgatcacgt aggcgggctt
      721 atatctgtac tgcaagaatt ccctgtcgaa atgatctata tgagcaaact cgaatattcc
      781 accactactt tcgacagttt tttgagcatt gcagctgata ataaggttcc cattatttat
      841 ctccaagaag gctcagagtt cgacctcggc agcaagatac atgcgaaagt atacaatccg
      901 gaagccaata tcgtgtacta tttcggatat cctgccaata gcactcaatt tgtaaacgat
      961 agatctctgg tagtaaaatt ggcttttggt aaatcgagca tactgttcat gggcgatgtg
     1021 tatacatcaa gagaacttga tcttatagag aaatatggat ctgaacttaa ggctgatgtt
     1081 ttgaaagtgg gacaccacgg tagtgataca tcctcctcca agtcttttat tagacttgtt
     1141 tgtccaaaaa ccgcagttat aatgcatgat cgccttgcta gtctccaagt atataacaat
     1201 cttaaaaaaa taggatcaga agtctatctg aattgcatcg atggaaacat aaggctctct
     1261 atcgacgaag agaagaatct gacgataatt acgcagttcg accgaatagt cgacttttaa
     1321 ggcaggaatt ctatactgtc ttcgcaatct taaaataaat tcttgccaaa aatatccata
     1381 ctgctacgaa ccttgtttat gaggattgag aatggcataa ggaagccttc tcatgcacaa
     1441 gcatagccag gcacataatc tgctttcttc ttatatatat acaccacaag tctatgcagc
     1501 atattggacg cattccttgc aaagcacacc gtttctctgc gatgatccgc aaggtgcttt
     1561 ctaagctctc tatcgagata gcaaacctgg ggcggttcaa tagatcgaag caaaccacaa
     1621 ggttcgtagc agtaaagatg tattggcaat ctgtctgagt aatggtatta taataaccat
     1681 caaaaagcgt tgttaacgca tggaataatc agatttgatt agcgtcaaca aagtcgaggg
     1741 ttcaattccc tatatctaca taagctaagt cgatattttt caataaactg cgttttgatt
     1801 gctaaccacg gttcagcctt tagagttaag aaaggtttgc atcccatttt cattacgatc
     1861 atgctcaaca aaatctgggg aggtctatac cattatgaga atgatagaaa ttcttaggga
     1921 catctggggg acagcaaaaa cagttctccc cttagccatg atcttagtga tactccaggt
     1981 cattgtcctc aagaagccca ttaagaatct cagagaattc attattggaa ttctgctgag
     2041 catcgcagga ttgcacttct tcttgaaggg cgtctttatg agtattctac ccttaggaga
     2101 ttctgttggt gggaatattg ctatattgga taacaaattg ctaatagtag ctatatcgct
     2161 tgttatcggt tatctaggaa cccttgtcga gcccgcgctc aaaacacttg cgctcgaagt
     2221 tgaagaagtg tctgtgggag ccataccgaa caaagtgcta attcatgcgg ttgccattgg
     2281 ttttgggctc ggaatgggaa ttggggtgtg gaaaataata aacaatatct cgtttataaa
     2341 aatcgtcatt cctcttatct ttgttatcat tttgctgatt ccttttgttc caaaagagtt
     2401 cgtatcgatt gccatggatt ctgcaagcgc tacgacaggg ccagtcaata ttccgttgaa
     2461 catggctatt gctgtaggtc tggccaaatc gctcgaaaat gtagatccgc tgacatctgg
     2521 ctttggtatc attggcctca cttcgattgg ggcggttatt tcagtgatgg ttctcggact
     2581 tttgacgaga atttgaacaa gcagataaag agggaaagaa ataatggaag tcaaatgcat
     2641 tgttgcaatt gtcgaaaggg gaaaagccga tgccatcgtg gaaaaagcca aaaaagcagg
     2701 agccgacgga gccacgattc tctatggcag aggcactggc caacctgaag ctctaagatt
     2761 cttcaacctt cacatagaag cttctaaaga agtgattata attctcagcg atacagataa
     2821 atacaagcca atctacgatg ctatcatcga agaaggaaac cttcacgaac ctggcacagg
     2881 gataatcttt accttt
//
LOCUS       DAOOWY010000175         2821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_35593_length_2821_cov_14.112075, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000175 DAOOWY010000000
VERSION     DAOOWY010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35593_length_2821_cov_14.112075"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /gene="pheT"
                     /locus_tag="PK105_08785"
     CDS             complement(<1..1330)
                     /gene="pheT"
                     /locus_tag="PK105_08785"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU61852.1"
                     /translation="MPKIEVNEEFFFQLAGQSWSNYEDLEQDLTIAKAELDEWDRIPS
                     DAGERMIKIELNDTNRPDLWTTAGLARQLRIYRTRNIPSYPFFASKGKSIDAAYRVVV
                     EKSVREVRPWIACFVAKGKPISDALLKDMIQTQEKLAWNYGRKRKGVSIGIYRTGLIE
                     WPVRYFGVEPDSVSFVPLQETRSMTLSQILKEHPKGVEYGSILEGKTIHPLLTDSKGS
                     VLSYPPIINSADIGAVQVDDSEVFIEVTGTDYPSVALSASIMACDFYDIGFEIANVRV
                     DYEYDSPFGSSVIFPYYFQNKVTLSLIEANRLLGDSIDLKKAQDALARMGLGAYCSDG
                     LTLHVTPPEYRNDFLHPVDVAEDIMIGLGIENFAPERPSDFTIGRLTPIEVFSRKAKD
                     IMIGLGYQEMIYNYLGSGIDYAEKMQIPVGQLVKIANPMTENYEYVRNSPI"
     gene            complement(1347..>2821)
                     /locus_tag="PK105_08790"
     CDS             complement(1347..>2821)
                     /locus_tag="PK105_08790"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOU61853.1"
                     /translation="RVKQVLGFREGQEQQAFSWLLAKGVLEEAERRTEVFYELTPLGQ
                     EWHEKGIPARRIFSLLKDRGPMSLPEIAQTLGFDQKTVGSAFGSLSKEAVCAIDEARR
                     ARLAKNEFPASLSEIESLLALAASSGGSVSEKALSDSQRALMGQIAKKRGAQDSPFRI
                     TDRVRVLYRVTPIAKEYRKAISTLGMTGEELGAITPEMLEKGTWKKGHFRPYNVQIPP
                     ARVIPGRRNAYVEFLEGVKDKLVSLGFVEFDGNLVETEFWNSDALFMPQFHSARDIHD
                     VYYIEEPTHAKFIEEPYLSNVAAAHENGGNTGSRGWQYQFDRDFTRRLILRSQGTVLS
                     ARQLPKAEVPGKYFGIVRCFRYDRVDATHLSDFYQTEGIVLGRNVNLKTLLGFLEMFA
                     VEVAGAKEVKYVPGYFPFTEPSIEVHIKHPVLGWFELGGSGIFRPEVSSSLGVEVPVL
                     AWGIGIDRMALMALGLNDLRDLFCSDLESVRMRRSQYKIQ"
BASE COUNT          731 a          717 c          616 g          757 t
ORIGIN      
        1 gaataggcga attcctgaca tactcatagt tttcggtcat aggattggct attttcacaa
       61 gctgaccaac gggtatttgc atcttttcag catagtctat acctgatcca agataattat
      121 agatcatctc ctgatagcca agacctatca ttatatcctt tgctttgcgc gagaatactt
      181 cgatgggagt aagtcttcct attgtaaaat ctgaaggtcg ctcgggagcg aaattctcaa
      241 taccaaggcc tatcatgata tcttctgcga catctacggg atggagaaaa tcatttcgat
      301 attcgggtgg agttacatgc aaggtgagcc catcgctaca ataagcgcca agccccatcc
      361 gagctaaggc atcttgcgct tttttcaaat cgatagagtc tccaagcagt cgatttgctt
      421 cgataagaga aagagttact ttgttctgaa aataataggg aaagatcacg gaggatccaa
      481 atggactgtc gtattcataa tcgacccgca catttgctat ttcaaagcca atgtcataaa
      541 aatcgcaagc catgatggaa gccgaaagcg ctaccgaagg gtaatcggta cctgttactt
      601 caatgaagac ctcggagtcg tcaacctgga ctgcaccgat atcagcgcta ttgattattg
      661 gcggatacga tagcacactc cccttagaat ctgtaagcaa aggatgtatg gttttgccct
      721 ctagaatgga tccatactcg acaccctttg gatgttcttt gagaatctga cttaaggtca
      781 tcgatctggt ctcctgaagc ggcacaaagg acaccgaatc tggctctact ccaaaatatc
      841 gaacaggcca ttcgataagc cctgttcgat agattcctat cgaaacccct tttcgttttc
      901 ggccataatt ccaagccagc ttttcctgag tctgaatcat gtcctttagc agagcgtcgc
      961 ttataggctt gccctttgca acgaaacaag caatccatgg acggacttct ctcacggatt
     1021 tttcgacaac aacccgatag gctgcatcga tactttttcc cttagatgca aagaaaggat
     1081 aagaaggaat attccttgtg cggtatattc gcaactgccg agccaatccg gcagttgtcc
     1141 aaagatccgg gcggtttgtg tcatttagct caattttgat catgcgctcg cccgcatccg
     1201 aaggaatcct atcccattca tccaattctg cctttgctat agtgaggtcc tgttcaagat
     1261 cttcataatt gctccagctt tgccctgcaa gttgaaaaaa gaactcttcg ttgacttcga
     1321 tttttggcat ggtttatctc cggacctcat tgtattttgt attgggatcg acgcatcctc
     1381 acgctctcca gatcgctgca gaacaaatct cgcagatcat tgaggccaag cgccataagc
     1441 gccatccgat cgatgccgat accccaggct agaaccggta cttcgacgcc aagagagcta
     1501 gatacctctg gtctaaaaat acctgaaccg cccagttcaa accatccgag cacaggatgc
     1561 tttatatgaa cctcgatcga aggctcggta aaaggaaaat acccaggcac atattttact
     1621 tccttggctc cagcaacctc gacagcaaac atctccaaga atccaagtaa ggtcttaaga
     1681 ttcacatttc tgccaagcac aatgccctcg gtttgataga aatccgacag gtgagtggca
     1741 tctacgcgat cgtatctaaa acagcgcaca ataccaaaat acttgcctgg aacctcagcc
     1801 tttggaagct gccgagcgga taacaccgtc ccctgacttc gaagtatgag ccgccttgta
     1861 aaatcccgat cgaattgata ttgccagcca cggctgcccg tattgcctcc attttcgtgc
     1921 gccgcagcga cattagatag gtatggctcc tcgatgaact ttgcgtgtgt cggctcttca
     1981 atgtaataga catcatgaat atcgcgggct gaatggaact gtggcatgaa aagtgcatcc
     2041 gagttccaga actcggtttc cacaaggtta ccatcgaatt ccacgaatcc tagcgaaact
     2101 agcttgtcct tgacgccttc caagaattcg acataggcgt tccggcggcc tggtatgact
     2161 ctggctggtg gaatctgaac attgtaaggt cggaaatggc ctttcttcca tgtacctttc
     2221 tcgagcatct ccggagtaat agcgcctaat tcctccccag tcatacccag ggtacttatg
     2281 gctttgcggt attctttcgc aataggagtg acacgataaa ggactctgac tctatcggtt
     2341 attctgaaag ggctgtcctg cgcgccgcgc ttctttgcaa tttgccccat tagcgctctt
     2401 tgagaatcgg agagcgcttt ttccgagact gaaccgccac tggaggcagc caacgcaaga
     2461 agtgattcga tttccgagag agacgcggga aattcatttt ttgcgagcct ggcgcggcgc
     2521 gcttcatcta tcgcacatac ggcttctttg gagagcgaac caaaggccga tcctacagtt
     2581 ttctggtcaa aacccagagt ttgagctatt tcgggcaggc tcattggtcc tcgatctttc
     2641 aatagagaaa atattctcct ggctggaata cccttctcgt gccattcttg cccaagcgga
     2701 gtgagctcgt aaaagacttc ggttcttctc tctgcttctt caagcacccc tttggcaaga
     2761 agccaggaaa aagcctgttg ctcttgacct tctctaaatc cgagaacttg tttgactcgc
     2821 t
//
LOCUS       DAOOWY010000176         2820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_35610_length_2820_cov_12.668354, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000176 DAOOWY010000000
VERSION     DAOOWY010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35610_length_2820_cov_12.668354"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK105_08795"
     CDS             <1..278
                     /locus_tag="PK105_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61854.1"
                     /translation="ALRTCSISIASCPLYVEKMNFVIEGEGKAPIAIKAKWNADAFDP
                     DGLQAFRKLHPNGVNLVVSANVDNAYTRTVSGLEVSFCSLAYLRQRV"
     gene            640..1638
                     /locus_tag="PK105_08800"
     CDS             640..1638
                     /locus_tag="PK105_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HOU61855.1"
                     /translation="MNLSTANILVTGADGFIGSHLVEALLDQGAKVRAFVFYNSFNSW
                     GWLDTIPEEKLKQIEVFTGDIRDPHGVQEAMKGIDIVFHLAALIAIPFSYHSPDSYVD
                     TNIKGTLNVLQAARNLGTHRVLVTSTSEVYGTAQYVPIDENHPFHGQSPYSATKIGAD
                     RLAESFYRSFGLPVTIVRPFNTYGPRQSARAVIPTIITQLLSGSTSIRLGSLQPTRDF
                     NYVKDTCAGFIEIAKHDATIGEEINIATQREISIGDLVAELVKQINPHARIVSDNTRI
                     RPEKSEVERLLGSNEKIRRLTGWFPQYSFEDGLAETIAWFSQPENLARYKADIYNL"
     gene            1672..>2820
                     /locus_tag="PK105_08805"
     CDS             1672..>2820
                     /locus_tag="PK105_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HOU61856.1"
                     /translation="MDNTHFEEILQFIRTLYPNENPVPLHAPRFLGREKEYLTQCIET
                     TYVSYVGQFVTDFEEHIKKLTGAHYAVAIANGTAALHMTLLAAGVQPGNEVITQALTF
                     VATAAGIKHALAEPIFIDVEAETLGMSPEALKAFLVENASYKNGVTINKKTGKRIAAV
                     VPMHTFGHPVRLEEISAICDEYGLMLIEDAAESLGSTYKGKHTGTWGRAGILSFNGNK
                     PVTTGGGGMIISNDEALAEKARHLSTTAKRKHPWQFYHDEVGYNLRLPNINAAVGCAQ
                     MEYFDSTIKNKRETAEMYKAFFDNKNIPFFTEPQDCHSNYWLNVMFLNDREERDAFLE
                     YSNANGIQTRPVWQLIPALPPYASCQCDPIPIAKDLEDRIVNIPSSVRI"
BASE COUNT          804 a          617 c          650 g          749 t
ORIGIN      
        1 aagccttgag aacgtgcagt atttcgattg cgagttgccc tctgtacgtc gagaagatga
       61 attttgtaat cgaaggcgag gggaaggctc ctatcgcgat caaagccaaa tggaatgcag
      121 atgctttcga tcctgacggt ttgcaggcgt tcagaaaatt gcacccgaac ggcgtcaacc
      181 tcgtcgtgtc ggcaaatgtc gataacgcgt acacccgcac cgtgagcgga cttgaggtca
      241 gcttctgctc gcttgcatat ttgcgccagc gagtataata gtgagtacct ttctggagtg
      301 aatatactac gattacgacc actatgcggg gcctggtgcg ccccaagcga ttatctcccg
      361 gtacagcaca accattttta tcatcattga ccgccatctt gcctcacata aactccttgc
      421 cactcggtct cagtatgttg cggccgcgat agagaggggg aaggggctag cagactcctc
      481 ttaatgagcc ttaactcaag tgaggatatt tgttttgcaa cattctgctt ttcaaatttt
      541 aaaaagcttg agtatcgata ttaataagat taactaaaag ctatgatgaa tcaccgtaaa
      601 gtttattccg agtaattttg tcaaataata aatgaattta tgaatttatc tacagcaaac
      661 attcttgtta ccggcgccga tggctttatt ggcagccact tggtagaagc ccttctcgat
      721 caaggcgcaa aggttcgagc atttgttttc tataacagct tcaattcctg gggctggctc
      781 gatactatcc cggaggagaa acttaagcaa atagaagtgt tcaccggaga tatccgcgat
      841 ccgcatggtg tccaagaggc aatgaaaggc atagatattg tctttcatct tgctgcgctt
      901 attgctattc catttagtta ccattcccct gatagctatg tggataccaa tatcaagggg
      961 actctgaatg tgcttcaagc agcgcgcaat cttggtactc atcgtgtatt ggtcacttca
     1021 acatccgagg tatatgggac tgctcaatat gtccccatag atgagaatca tcctttccat
     1081 gggcaatcac catactctgc gacaaagatc ggtgcagatc gtttggctga gagcttttat
     1141 agaagctttg ggcttcctgt aacgattgta agacccttta atacttatgg tccgcgacag
     1201 tctgcacgtg cggtcattcc aacaattatt actcagctat tgagcggttc gacatcaata
     1261 cggcttggga gtctccagcc aacgcgggat tttaactatg taaaggatac atgtgctggt
     1321 tttatcgaaa ttgcaaaaca tgatgcaact attggcgagg agatcaacat agcgactcag
     1381 cgcgaaatat ctatcggtga tcttgttgca gagcttgtga agcagataaa tcctcatgca
     1441 aggatcgtat cggataatac gcgcattcgc cctgaaaaga gcgaagtaga aaggcttctt
     1501 ggttcgaatg agaaaattag aagattgaca ggctggtttc cacaatattc attcgaagac
     1561 ggcctcgcag agacgattgc atggttctca cagccggaga atcttgcgag atacaaggcg
     1621 gacatttaca atctttgaat tgattaaaaa gcaaacaaga aggcaatgaa catggataat
     1681 acccactttg aagagatcct tcaatttatt cgcacactgt atccaaacga aaatcccgtg
     1741 ccgctccatg cgccgcgatt tcttggcaga gagaaggaat atctcaccca atgcattgaa
     1801 acgacgtatg tctcatatgt agggcaattt gtaacagatt tcgaggagca tataaaaaag
     1861 cttactggcg ctcactatgc tgttgccatt gcaaatggaa cagctgcatt gcatatgaca
     1921 ctccttgctg caggtgtgca gcctggtaat gaagtgatca cacaagcgtt gacatttgtg
     1981 gctacggccg cgggtataaa gcatgcgctt gcagaaccga tctttatcga tgttgaggcc
     2041 gaaactcttg gtatgagtcc agaagcgctt aaggcattct tagtagaaaa tgcaagctat
     2101 aaaaatggcg tgacaataaa taaaaaaaca ggaaagcgga ttgctgcagt cgtccctatg
     2161 cacacctttg gtcatcctgt acgcctcgag gaaatttcag caatttgcga tgaatacggt
     2221 cttatgctca tagaagatgc agctgaatcg ttaggcagta cttataaagg aaagcatacc
     2281 ggtacgtggg gccgagccgg tatactttcc ttcaatggca ataagcctgt gacaacgggt
     2341 ggtgggggca tgatcatttc aaatgatgag gcacttgcag aaaaagcccg tcatttgagc
     2401 acgaccgcaa aaagaaagca cccatggcaa ttctatcatg atgaagtcgg atataacctg
     2461 cgtcttccta atattaacgc agccgtaggt tgtgcccaaa tggagtattt cgattcaacg
     2521 atcaaaaaca agcgtgagac tgcagagatg tacaaagcct tctttgacaa caagaatatc
     2581 cccttcttta ccgaacctca agattgtcat tccaattatt ggctcaatgt gatgtttctt
     2641 aacgataggg aagagcgcga cgcattcctt gaatattcaa atgccaacgg gatacaaaca
     2701 aggcctgttt ggcagctcat tcctgctttg cctccgtatg cttcatgtca atgtgacccc
     2761 attcctatcg caaaagacct tgaagacaga attgtgaata ttccgagcag tgtgagaatt
//
LOCUS       DAOOWY010000177         2793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_36052_length_2793_cov_8.487217, whole genome shotgun sequence.
ACCESSION   DAOOWY010000177 DAOOWY010000000
VERSION     DAOOWY010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36052_length_2793_cov_8.487217"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1345
                     /locus_tag="PK105_08810"
     CDS             44..1345
                     /locus_tag="PK105_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOU61857.1"
                     /translation="MGAYAFKSWSHMLPAERAKYLEKFAQLLDQEKQRIGEALCREEG
                     KTIKEAVGEPSRGAVETSFFIGEGQRLEGITMPSDRKGVVSVATRVPIGVVAAISPWN
                     FPFLTPVRKVIPALVFGNTVVFKPASDTPYCAVLLMELFEKAGLPPGVVNLVIGKGSE
                     IGDVISSNPLVNGITFTGSTVVGRRINQLASEHFAKVQLEMGGKNPAIVADYKDLNRA
                     AAEIAQAAYAVSGQRCTAVSRVIVLKEHAEELETKIIEKAKSHILGNGMRPDVTMGPI
                     ITKEAGEKIMDYIHGAEKEGAMIKYGGRHLTGGEFDKGFYIEPTLITDVTPSMKVARE
                     EIFGPVLVSIKVDTFEEAIEVANNTEYGLASCLFSDNLKYIYEFQQEIESGMTHVNHG
                     TVTDASMPFGGAKNSGLGPFSKGATNKDFYTNYKVNYVKYI"
     gene            1417..1893
                     /gene="aroQ"
                     /locus_tag="PK105_08815"
     CDS             1417..1893
                     /gene="aroQ"
                     /locus_tag="PK105_08815"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOU61858.1"
                     /translation="MALKSGMLMESNHIAIIHGPNLNTLGQREIGIYGGKTLEEINDE
                     IRLEASKFPVSLDFFQSNVEGFIIDFIHGCRGKIDGIVINAGAHTHYSIALRDAIAAV
                     KLPTIEVHISNIYKREAFRHVSIISPVCIGQICGFSSYSYILGLHALLEVLAEKDK"
     gene            1929..2366
                     /gene="aroQ"
                     /locus_tag="PK105_08820"
     CDS             1929..2366
                     /gene="aroQ"
                     /locus_tag="PK105_08820"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOU61859.1"
                     /translation="MKKFLSISGPNLNLLGQRKTSIYKEASLDEIHSKLKKKAAELGV
                     EVDCVQFNSEGDIIDCMQTVPGKYEGVILNAGALAHYSYALRSAIGTMLVPCVEVFIS
                     NVYAREDFRHKSVLADVCVGVISGFGANSYILALEALVNLVKP"
     gene            2435..>2793
                     /locus_tag="PK105_08825"
     CDS             2435..>2793
                     /locus_tag="PK105_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61860.1"
                     /translation="MNMRWAKRVDRFLDIGQAKEQGFQIVQLPVAEIMRLDDRAFRDV
                     KIDLLESGLSFEVFESPLPEGVNTTERGFNIYAWIEYLKKAIGRISELGCKTLVWGDG
                     QARILPEEGEATVLKEN"
BASE COUNT          837 a          530 c          701 g          725 t
ORIGIN      
        1 acttccctct ttctactaaa aatgacgttg atgaggcaat acaatcggcg catatgcctt
       61 taagagctgg tcgcatatgc tgccagcaga gcgtgcaaag tatcttgaaa aatttgcaca
      121 gcttcttgat caagaaaagc agcgcatcgg agaagcattg tgcagagagg aaggcaaaac
      181 cataaaggaa gctgtgggag aaccttcgcg aggtgcggtg gagacctcat tttttattgg
      241 agaaggccag cgtcttgaag gaattaccat gccgagcgac cgcaagggag tagtttcagt
      301 cgctactcgc gttccgattg gagtggtagc agcgatttcg ccgtggaatt tcccttttct
      361 tacccctgtg cgcaaggtta ttccagcgct tgtattcggc aatacagtag tcttcaagcc
      421 agcttcggat actccctatt gcgctgtgct cttgatggag ctcttcgaaa aagcaggcct
      481 cccgcccggt gtagtaaatc ttgtaatagg aaagggaagc gaaataggcg atgtgatctc
      541 ctccaatcca ctagtaaatg gcataacctt tacaggatca acagtcgttg gacgaagaat
      601 caatcagctt gcctctgagc attttgcaaa ggttcagctt gaaatgggag gaaagaatcc
      661 agcgattgtg gctgattata aagatctcaa tagagcagcg gcggaaatcg cgcaggcagc
      721 ctatgcagta agcgggcagc gctgcacagc ggtaagccgt gttatcgtat tgaaagaaca
      781 tgcagaagaa ctagagacaa agataattga aaaagcaaaa agccatattc tcgggaatgg
      841 aatgcggcca gatgttacca tgggtcccat tattacaaag gaagctggcg aaaagataat
      901 ggattatatt catggagcag agaaagaagg agcgatgatc aaatatggag gtcgccatct
      961 tactggcgga gaattcgata agggattcta tatagagccc acgctcatta ctgatgtcac
     1021 gccctcaatg aaggtagctc gagaagaaat ctttggtcct gtgcttgtca gtattaaagt
     1081 cgataccttc gaggaggcga tcgaagtcgc caataataca gaatatggtc ttgcctcttg
     1141 tctattttcc gataacctaa aatacatcta tgagtttcag caggaaatag aatccggaat
     1201 gactcacgtt aatcatggaa cggttaccga cgcctctatg ccatttggag gcgcaaagaa
     1261 ttctgggctc ggtccatttt ctaaaggtgc tacgaataag gatttctata caaattacaa
     1321 agtcaactat gtgaaatata tctaaacaag atacctcata agcagcataa gcaggcgttg
     1381 cgctacatat tcctcgttta agaataatag agtacaatgg ctcttaaatc aggaatgctt
     1441 atggaatcga atcatattgc gataattcat ggtcctaacc tgaatacgct tggacagaga
     1501 gaaatcggaa tttatggtgg caagactctg gaagagatca atgatgaaat tcgattagag
     1561 gcatcgaaat ttccggtcag cctggatttc tttcaatcga atgtcgaggg attcattatc
     1621 gattttattc atggctgtag agggaaaata gatggaatcg taatcaatgc cggtgcccat
     1681 actcattact cgattgcttt gcgcgatgca atagccgctg tcaaacttcc aacaattgaa
     1741 gtacatatat cgaatatcta caaaagagag gcttttcggc acgtatctat catctcgccg
     1801 gtatgcatcg ggcagatatg cggctttagc tcttatagtt acatacttgg tctccatgca
     1861 ttgctcgagg tccttgccga aaaggacaaa taatattcat cgataagttc agagaggaga
     1921 aaagagaaat gaagaagttc ttatcgattt ctgggccaaa tctaaatctg ctggggcaaa
     1981 gaaagacttc tatttataaa gaggcttcac tggacgaaat tcattctaag ctaaagaaaa
     2041 aggctgcaga actaggtgta gaagtggatt gcgtgcagtt caatagcgaa ggcgatatca
     2101 tagattgcat gcagacagtg cctggcaaat atgagggggt tatcttaaat gcgggagctc
     2161 ttgcacacta cagttatgct ttgcgcagcg caattggaac gatgttagtg ccctgtgtag
     2221 aggtttttat atcgaatgtg tatgcgagag aggattttcg gcataaatcg gtccttgccg
     2281 acgtatgtgt tggagttatc tcgggattcg gtgcaaacag ctatatcctg gcgcttgaag
     2341 cacttgttaa tctggttaag ccttgaagaa agctctgata taagttttag cggaagcctc
     2401 aaattgagga gcggctatga atggggaagc cactatgaat atgagatggg caaaaagagt
     2461 tgatcggttt ttggatatag gacaagccaa agagcaggga tttcagattg tgcaactgcc
     2521 tgtggccgaa ataatgcggc tcgatgatcg tgcatttcga gacgtaaaga ttgatcttct
     2581 tgaaagcgga ttatcatttg aggtatttga atccccgctt ccggaaggtg tcaatactac
     2641 cgagagaggc tttaatatat atgcgtggat cgaatatctg aagaaggcaa ttggaagaat
     2701 ttctgaacta ggatgcaaga cattggtttg gggagatggc caggcgagga ttcttccaga
     2761 agaaggcgaa gccactgtcc taaaagaaaa ttt
//
LOCUS       DAOOWY010000178         2780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_36277_length_2780_cov_10.656881, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000178 DAOOWY010000000
VERSION     DAOOWY010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36277_length_2780_cov_10.656881"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..1458
                     /locus_tag="PK105_08830"
     CDS             427..1458
                     /locus_tag="PK105_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61861.1"
                     /translation="MSIYDQRDRPAYERFIINIGYASNDYILRWWTEKHDQLLAVEIN
                     TWQWVWYWKITDCIIAITPPNVIEQWKLDDPLCSRFAWYNIIMYFAAARAQQLHLTDL
                     IRFPVWKTCPLCGQPFSEDSLPFPLIDRLGIDHLEFCAPCLRDTILARGSDKVTKQDI
                     LEFASKLTDVLGLVPPQGFGSGTRDLDGLDYTTRIALLKLLMYKPSTKSIKNFFGSWL
                     NVLTEAGVLEDGTRKRSRGIQTIARDGHVCLSLGEKTIDDWLYAHHITHEKEPKYPEY
                     NFRADFKVGNLFIEFFGLVGDPDYDKRIAQKKRLCLENNIELLAIYPKDLAASDGLSN
                     ILTPLLQLA"
     gene            1867..2013
                     /locus_tag="PK105_08835"
     CDS             1867..2013
                     /locus_tag="PK105_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61862.1"
                     /translation="MIANTHKLKIISFTDKETDKVDAEKLARIIKMQVLSGEQQVHPI
                     PLTV"
     gene            2039..2560
                     /locus_tag="PK105_08840"
     CDS             2039..2560
                     /locus_tag="PK105_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU61863.1"
                     /translation="MEKLTSDILIAAVPFIHEIDILTSMKGISVIAAAAVMSDIISVG
                     HFQDSKHFAYHLRSTLGVQSLNEKMIIKRTNKAERKMTMVFLSQCAYHCRDADQNLSR
                     WYDKLTEYKKKGLVRMGLCRRVITQLYQMLKKDEYCYHMDTTNHDTKMTVYRSFLAKQ
                     GVRIEGDAYKIAS"
BASE COUNT          781 a          639 c          618 g          742 t
ORIGIN      
        1 cggtaggctt cgacgatttc aagaagtgta cgaccggttt tcttttcatg gagctttcct
       61 aagaacataa gggcctccta tgaggagagc atagcacacc tctgaactca atacaaccca
      121 ttacgtatca tttcttcaaa tatttttaaa aaatattccc ttcagattgt gctgaaagga
      181 attatttccc ctagtccctt atgtcaactg taaaagtacg gataaaggtc ctctgtgaag
      241 gtgagtttgg tattaccact attatagggc ctttatgagt atcaattgct tgaattgatt
      301 cagctgcttg tcatggttta tgctaggata ctgatgcaca aactacgcca gcctcgcttc
      361 acaggttaag cgtaggttat gcgcgttgaa aatttttgtt actctatatc ggagcgataa
      421 aggtccatga gtatatacga tcaaagagat aggcctgcct atgagcgttt tatcatcaat
      481 attggctatg ctagcaacga ttacatttta cgttggtgga ccgaaaagca tgatcagcta
      541 cttgctgttg aaatcaacac ttggcagtgg gtttggtact ggaagatcac cgactgtatc
      601 attgctatca ctccgccaaa cgtgatcgaa caatggaagc tcgatgatcc gctctgctcg
      661 agattcgctt ggtacaatat catcatgtac ttcgccgcag ctagagcgca acaacttcat
      721 ctcaccgacc tgattcgatt ccccgtttgg aaaacctgtc ccctttgcgg acagcctttc
      781 tccgaagatt ctcttccgtt tcctctcatt gatcgacttg gtattgatca tctagaattt
      841 tgtgcgccct gtctccgaga cacgatactt gcacgaggca gcgacaaagt aaccaagcaa
      901 gacatcctgg aattcgccag taagctaact gatgttctcg gcctagtacc accccaggga
      961 tttggatcag gaacgcgtga cctcgatgga ctcgattaca ccacgagaat cgcactactt
     1021 aagctcctta tgtataagcc ttcaacaaaa tcaattaaga attttttcgg atcttggtta
     1081 aacgtcctta ctgaggcagg cgtcctcgaa gatggtacca gaaagagatc tcgcggaatt
     1141 cagacaattg cccgagatgg ccatgtctgc ctttcgttgg gtgaaaagac tatagatgac
     1201 tggctctatg cccaccacat cactcatgaa aaagaaccaa agtacccaga atacaatttc
     1261 agagcggact tcaaagtcgg caaccttttc atcgaatttt ttggtctagt aggtgacccg
     1321 gattatgata aaaggattgc ccagaaaaaa cgactttgcc tcgagaataa catcgaacta
     1381 cttgcaatct acccaaagga tcttgcggct tcagatggcc tctccaatat actcacaccg
     1441 ctgcttcaac tcgcataata tgtgattgat gttgttgtgc ttggtaaacg acctactaca
     1501 ctccaggaat agcttcatct cgcgttgtta gcattgaaat gcacagcgac agcacacagt
     1561 ttatccagta gtcttctctt ggattgaatg taacagaagt tatgataatt aagatgaggt
     1621 tttcggtata ctatggcatc cttcgacgaa ggaggttggc agctgataat tgaactggga
     1681 cggaagatcc cgtgtaagcg aatagcagcc ggcaaccgca tcaacttaat gaggatgccc
     1741 agacaggggg cgtaccccga gtagacgttt gattgaaaag gcttcgttcg aagagataca
     1801 cgggaggtac catgagattt ttaggaatcg acctgcatac ggattgcgat cgcagttgcg
     1861 gaagtcgtga ttgcgaacac ccacaagctc aagataatca gctttacgga taaagaaacc
     1921 gataaggtag acgctgagaa gcttgccagg atcatcaaaa tgcaggtgct ctccggggaa
     1981 cagcaggtac atcctattcc tcttactgtt tgacagtctt gaacacttgg aaatgagtat
     2041 ggaaaaattg acttcagata ttctcattgc tgctgtgccc tttatacatg agatcgatat
     2101 tcttacgagt atgaagggta tatcggtgat agctgcagcc gcagtcatgt cggacatcat
     2161 ctccgtaggc catttccagg attcgaagca ttttgcctac catctccgga gtacgctggg
     2221 cgttcagagt ttgaacgaaa aaatgatcat caagcggacc aacaaggctg aacgaaaaat
     2281 gacaatggtg tttctctccc agtgtgccta tcattgccgt gatgcagacc aaaacctttc
     2341 tcgctggtac gacaagttaa cggagtacaa gaagaaaggg cttgtcagaa tgggactctg
     2401 cagaagggtg atcacccaac tctatcagat gttaaagaaa gacgaatatt gctatcatat
     2461 ggacacgacg aatcatgata ccaagatgac agtgtaccgc tcctttcttg ccaaacaagg
     2521 cgtcaggata gaaggagatg catataaaat tgcatcttga caattctcat agacgtttct
     2581 taccaccaga tgcttacctg cttcatcctt ccggtcctgg tatcgtgcaa gatgtgcctc
     2641 tatttctgct tcaagtgctg cgtgcaacaa tcatcgtgct cctgcaagcg gtatgatctg
     2701 ccccccaaaa aggtaacaca tcagtggcca ctgcacccct ttctggtgac actactggag
     2761 gggccatgaa caaagatccg
//
LOCUS       DAOOWY010000179         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_36375_length_2774_cov_13.956969, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000179 DAOOWY010000000
VERSION     DAOOWY010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36375_length_2774_cov_13.956969"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..2250)
                     /locus_tag="PK105_08845"
     CDS             complement(136..2250)
                     /locus_tag="PK105_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOU61864.1"
                     /translation="MDSSKTPGGSLRAPIRNIGILAHIDAGKTSITERILHVTGVREK
                     AGNVDDGTTTTDYLTVERQHGITIKTAAVRFVHKGVLFNLLDTPGHVDFSAEVDRVLR
                     VLDGAVIILCAVSGVQVRTGFIATGCRAHHIPRIYFINKMDRRGADFFSAVKEIAGEL
                     GEPVLPLQIPTFEDYQWNGIVDLIDMSWYPALSNEEENESENKGDKIATASSEHHFPI
                     EKAPVTEEMRRLALESREKLLDRVSMYDDSLMEKLISGRTVSEKEIRSALIAPIWKQE
                     VTPILCGSSFSDISSRLLMDAIIDYLPDPYSRGCPPAKDMKTGKEIRLEAKPDAPFSA
                     YIFKTAFDADLNTLAWIRLWSGQLREGMKVMAMPFKKIAQMKHLYGINGAEIEKIDSA
                     AAGEIVGACLSYMEAGSTLCETHLQLVYESFKNPEPLVSIALEPTSQQDLPRLRNALK
                     RFSLEDAGLLIDEEKETGRITIAGQGELHLDIVLERLQEEYGLRVRAGNPQVPKVERL
                     MKPVHIEEEFTGDFGGEKLSVTLGISLENQKDKNENKVIFAQGLRLLANFEAALHRAA
                     ETSLAVGPSGGWPIIGARLIIERITAPMGGDRRAETAVEVATSAILRKAVSLAGSYIL
                     VPIVQVSVEVPDAWFGAALASLQARGARIEAVEDEGVVKSIIAYAPMENFFGYTTSLR
                     SITEGRGIYQAKFDHYGNMNEY"
     gene            complement(2414..>2774)
                     /gene="rpsG"
                     /locus_tag="PK105_08850"
     CDS             complement(2414..>2774)
                     /gene="rpsG"
                     /locus_tag="PK105_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOU61865.1"
                     /translation="VCTGIMYDALDLLQQRSGQPALDAFTKALENVKPSIEVKSRRVG
                     GATYQVPIEIRESRREALAMRWIIQAARARTGKSMSECLADEFFDAFNNTGAAIKKKD
                     DMHKMAEANKAFAHYRW"
BASE COUNT          702 a          690 c          622 g          760 t
ORIGIN      
        1 ttacttgttt gagcgttcgg attataagca aagaattgag attgcgcaat agccctatag
       61 aagaaaagct ctttttttct ttcctgtaaa atatatgtgc ctatatgtca tgaaatatgg
      121 atatagaaat ccatctcaat attcgttcat attgccataa tgatcgaatt ttgcttggta
      181 aatgccgcgc ccttccgtaa tggatctcaa agaggtggta tatccaaaga agttttccat
      241 aggtgcatag gcaataatgg atttgacgac tccctcatcc tccacagctt ctatgcgggc
      301 tccccgagcc tgcaatgaag ccagggcagc tccaaaccac gcatccggta cttcgacaga
      361 aacctggaca attggtacaa gtatatagct accagcaagg ctcactgcct tgcgcagaat
      421 cgcagatgtc gcaacttcca ctgcggtttc tgctcgtcta tcgccgccca tgggagctgt
      481 aatccgttca ataatcagtc tagccccaat aattggccag ccaccggatg gtccaacggc
      541 taaagaagtc tctgctgccc gatgcagagc tgcctcgaag ttagccaaga ggcgcaatcc
      601 ctgtgcaaaa atgaccttgt tttcgttctt gtctttctgg ttttccaatg aaattcctag
      661 ggtcaccgaa agtttttcgc caccaaaatc tcctgtgaat tcttcttcga tatgaacagg
      721 tttcatgagt cgctcgacct taggaacctg gggatttccc gcccttacac ggagtccgta
      781 ttcttcttgc agacgctcga gtacgatgtc aaggtgtaat tctccttgac ctgctattgt
      841 tattcgaccg gtttcctttt cttcatcgat caatagacct gcatcttcaa ggctaaatcg
      901 cttaagggca ttacgcagtc ttggaagatc ttgctgggaa gttggttcca gcgctatgga
      961 taccagaggt tcgggatttt tgaaagattc atagacgagc tgaagatgag tctcacagag
     1021 agtactacct gcttccatat aggacagaca tgcgcctact atttcccccg cagccgctga
     1081 atcgatcttc tcgatttctg ctccgtttat cccatagagg tgcttcatct gagcaatctt
     1141 tttgaagggc attgccataa ctttcatgcc ctcgcgaagc tggcccgacc atagacgaat
     1201 ccatgccagg gtgttgagat cggcatcaaa ggctgtcttg aaaatataag ctgaaaatgg
     1261 agcgtcaggt ttagcttcca gtctgatctc tttcccggtt ttcatatcct tcgcaggcgg
     1321 acagccacga gaataaggat caggaagata atcgattatc gcatccataa gaagccggct
     1381 ggaaatgtcg gaaaaggaag aaccgcatag aattggtgtc acttcctgct tccagatggg
     1441 tgcaataagc gcggatcgga tttccttttc agagacggtt ctcccgctta tcagtttttc
     1501 catcagggaa tcatcataca tgctgacacg atcgagaagc ttttctcgag attctagggc
     1561 aaggcggcgc atctcctcag taacaggagc tttttctata ggaaaatgat gttcgcttga
     1621 tgcggtcgca atcttatcgc ctttgttttc tgactcgttc tcttcttcgt tcgacaaggc
     1681 aggataccag gacatatcga taagatccac aatcccattc cattgataat cttcgaaggt
     1741 cggtatctgg aggggcaaaa caggttcacc aagttcacct gctatctctt ttacagcgct
     1801 gaaaaaatcg gctccccgcc tgtccatctt gtttatgaaa tagattcgcg ggatatggtg
     1861 agctcggcag ccagtagcta taaagccagt cctaacctga acaccggata ccgcacaaag
     1921 aatgatcaca gcaccatcaa gaacacggag caccctgtct acctcagcgc taaaatccac
     1981 atggcctgga gtatccaaca gattaaagag gactccttta tggacaaagc gcacagccgc
     2041 tgttttaatt gttattccgt gttggcgttc aacggtcaaa tagtcagtag tcgttgtgcc
     2101 atcgtcgaca tttccggcct tttcacgcac tcctgtgaca tgcagaatac gctcggtaat
     2161 cgatgtcttg ccggcatcta tatgggcgag aatacctata tttctgatag gcgccctgag
     2221 gctcccccca ggcgtctttg atgaatccat tgcaatatca atatatcaga gctattacaa
     2281 agataaaagc attactgtaa ggatgggcac acgcttccta tggcaaatat tagctcccta
     2341 ccagcggtag tgagcaaaag ctttattcgc ttaacggctg agcttcgctc aacctatagc
     2401 ctgcatttgc ccattaccat cggtagtggg caaatgcttt attagcttca gccattttgt
     2461 gcatgtcgtc tttcttcttg attgcggctc cagtgttgtt aaaggcatcg aaaaactcat
     2521 cggccaaaca ttcggacatg gacttaccag ttctggcgcg ggcggcctgg attatccagc
     2581 gcatcgcaag agcctctcga cgcgattcgc gtatctcgat cggaacctga taagtagcac
     2641 cacccacacg tcgggacttg acttctatag agggttttac attttcgagc gctttggtaa
     2701 aagcatccag agccggctgt ccggatctct gctgaaggag atctagggca tcatacatta
     2761 tgccggtgca gaca
//
LOCUS       DAOOWY010000180         2757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_36693_length_2757_cov_11.443745, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000180 DAOOWY010000000
VERSION     DAOOWY010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36693_length_2757_cov_11.443745"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..388
                     /locus_tag="PK105_08855"
     CDS             221..388
                     /locus_tag="PK105_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61866.1"
                     /translation="MASKVNIQILATSDLHGWFAPWDFATDTKDTKGSLAYLATLIDK
                     HRAHLIEQANA"
     gene            complement(618..1919)
                     /locus_tag="PK105_08860"
     CDS             complement(618..1919)
                     /locus_tag="PK105_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU61867.1"
                     /translation="MQLYDRLYASSFSHKISHPFVHIMFGARQTGKSTLIRSMIPPES
                     LVFDLSNPSQRLQFAANPGRFIDMYRALPSSESPRFIFLDEVQSVPELFDAVQFLYDE
                     EKRSAPDERRTRFILCGSSARRLRRTGTNLLPGRSILHHLYPLTSIEYQPFIPPGFPV
                     ETLYGSRSLVPIFKDEPRLVSPFPKRTLEDRMIYGELPAMAIPYAERGTNADPRRELL
                     RSYVAAYIEEELHRETQIREWGPFLRFLSLAAYESGGIMNFAGIAKESGLSAPTVKAH
                     YQLLEDMFLGFMVPAFSGSSRKTALSTPRFFFIDLGLRNAAAGLSLTPDTLLANPGPL
                     FEQWVGIELYKRLSYLGDSQLSYFRTAGGAEVDFIIEQSGILYPIEVKWTENPTTKDA
                     RHLESFLQDHRDRTSHGYIVCRAPYPLAVSNTVTAIPWWLL"
     gene            2349..>2757
                     /locus_tag="PK105_08865"
     CDS             2349..>2757
                     /locus_tag="PK105_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61868.1"
                     /translation="MPKYLFTPMRIGKMEVSNRLVVPAMVMNFCNNDGTATEKYIAYH
                     EAKAKGGWGLIITEDYAVDPRGKGFIGIPGLWNDDQIESHSKLPERVHRYGSKIVAQI
                     YHAGRQTNHLVIGAQPVAPSPIPCPANQEIPHEL"
BASE COUNT          750 a          592 c          745 g          670 t
ORIGIN      
        1 ttaaccaagc tttttcctca tgcactggta tcgtcgttga ggatatccgc tgtgcaacca
       61 aggatgatat tacccttcgc aatctagaaa cgtttagacg gctcgatgaa gattcattga
      121 gcctttctga aagtacgggc gcagctgtca tataattggc ggattaggat agaaatcttt
      181 agaaaacctg tttttctgct cgcatgtttt gacggttgag gtggctagca aggtcaatat
      241 tcagattctc gccaccagtg accttcatgg ttggtttgca ccatgggatt ttgctactga
      301 cacaaaagat accaaaggca gcctggctta cttagcgacc ttgatcgata agcatcgtgc
      361 tcatttgatc gaacaggcga atgcataaat catgtccgga aatgtccaca tgaaatcgac
      421 aggcgaaaga tatctgcctg catggactat catcgaacgt gagggcgcta aaattggaat
      481 catcggcgtc atgtatatgg gacttgatga agagaacaac gtgagggttc tcggtgtggt
      541 cgacttgttc ttcgaaaata ccgatactgt ctggaagatc gtgtccaaga gctcgaggga
      601 cgagccttgt ggctcagtca gagtagccac cagggaattg ctgttactgt attgctcact
      661 gcgaggggat agggagcgcg gcacacaatg tagccatggg aagtacgatc gcgatggtcc
      721 tggaggaagc tttccaggtg gcgcgcgtct ttggtggtgg gattttctgt ccattttact
      781 tcaatgggat agaggatacc tgattgctca atgataaaat cgacctccgc tccgccggca
      841 gtacgaaaat acgaaagctg cgaatcacca agatacgaga ggcgcttata gagctcaata
      901 ccgacccatt gctcaaagag agggcctggg ttggccaaaa gcgtatctgg tgtcagcgag
      961 agacctgccg cggcattgcg aaggcctaaa tcgataaaga agaatcgagg ggtggacagc
     1021 gcggtcttgc gggagctccc tgaaaatgca ggtaccatga agccaagaaa catatcctca
     1081 aggagttgat agtgcgcttt gacggtgggg gctgagaggc cagattcctt tgcgatgccc
     1141 gcaaagttca taatgcctcc tgattcgtaa gccgcgagag atagaaagcg caaaaatggg
     1201 ccccattcac gaatctgcgt ttcgcgatgg agctcttctt cgatatacgc tgcaacatag
     1261 gaccgcaaaa gctcgcgccg tggatcagcg ttcgtaccac gctctgcata ggggatggcc
     1321 atggcaggga gttctccata tatcatccga tcttcaagcg ttcttttagg aaaaggggat
     1381 acgagccgtg gttcatcctt gaaaatgggc acaaggcttc gagatccata gagcgtttct
     1441 actggaaaac ctggtgggat aaatggctgg tattcgatac tggtgagagg atagagatga
     1501 tggagtatgc ttctacctgg gagcagattg gtaccggtgc gccgtaatcg tcgtgccgag
     1561 ctgccacaga ggataaagcg tgtacggcgc tcgtcgggcg cactgcgctt ttcttcatcg
     1621 tagagaaact gaaccgcatc gaaaagctcg ggcacgcttt gcacttcatc gagaaatata
     1681 aagcgagggc tttctgatga gggtagcgct cgatacatat cgatgaatcg gccagggttt
     1741 gcagcaaact gtagccgctg ggatggattg gacaagtcaa agacaaggct ttcgggtgga
     1801 atcatactgc ggatgagggt cgactttccc gtctggcgtg caccgaacat aatatggaca
     1861 aagggatgcg aaattttgtg ggaaaatgag cttgcataaa gccgatcata gagctgcata
     1921 caatgagtat ggtgcaaact cctcgaaaag taaagtggca ctttagattg tttgagtaaa
     1981 acgaaataaa acattctgta attcctgcaa aatgctgcta tctcctatgt tttaggtcaa
     2041 gttaaaaagt acgggcaagt tgaaattgcc cgtatccgac aaggggcgaa ccaatgtcgg
     2101 ccccatcacc ataaaggatg tcgagccatt tctctgacat tcattgatac ccgacacctt
     2161 gtgggttccc catgaggtgc cggtagtttt ggcaaaaaga aagagaaaaa gttaaggcca
     2221 agagagagcg ctcacattgc ttccatgatt tcttatcgat ataattttat aagtgcattg
     2281 tcttatacaa tgccttattt cttttgctga tggcaaagcg aaagcttgcc gagaaaggga
     2341 ggtaaaatat gccgaagtac ctttttacgc ctatgcgcat tggcaaaatg gaggtaagca
     2401 accgattggt cgtaccagcc atggtgatga atttctgcaa caacgatggc acagcaaccg
     2461 aaaagtatat cgcgtaccat gaggcaaaag cgaaaggtgg atggggactc atcatcactg
     2521 aggattatgc agttgatcca cgaggaaagg ggtttattgg aattcccggg ctgtggaatg
     2581 atgatcagat tgagagccat tccaaattgc ccgagcgtgt tcatcggtat ggcagcaaga
     2641 tagtagcgca gatatatcat gcgggcagac agactaatca tcttgtaatt ggtgcacagc
     2701 cggtagctcc ttcgccgatc ccatgccccg cgaatcagga gattcctcat gagctca
//
LOCUS       DAOOWY010000181         2754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_36751_length_2754_cov_9.365691, whole genome shotgun sequence.
ACCESSION   DAOOWY010000181 DAOOWY010000000
VERSION     DAOOWY010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36751_length_2754_cov_9.365691"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..1244
                     /locus_tag="PK105_08870"
     CDS             429..1244
                     /locus_tag="PK105_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOU61869.1"
                     /translation="MRTKMPLNIRKFGRKKKELDRDSVQLILEVNKEQNLGARRLEKI
                     IEFKYGKHIPHNAIHRVLLAHGRANVNRNKAKRRKPWVRWERDHSLTLVHLDWHDSDI
                     NGKKVCAVLDDSSRRILAGGEFDEETSDNSIRLLQEALDNFEWLTSISQVMTDHGTQF
                     YANKRDKNGNADCGFENFLKRNKIKHILAPVKRPQVNGKFEKWNDTYEKNRIRFDNFD
                     EFMNWYNTVRFHESLDTKWYLQTPDMAFWARLPEGCKLGMFLKYMETELNGGN"
     gene            1519..2646
                     /locus_tag="PK105_08875"
     CDS             1519..2646
                     /locus_tag="PK105_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOU61870.1"
                     /translation="MQSLEPVINYLVDPNSGLSQVITEFLNAVMQYEAYLQSGALPYE
                     RCPQRKAHRNGTRPRSLKTRVGEIVLDKPQFREIPFESSIFERYCRVEQALLMTIAES
                     YIQGVSTRKMEELVKSFGLNRLSASEVSRLCKILDEKVEEFLKRPIEVKIRYLVVDAT
                     YFKVRCGVQYKNRALLIVVGIREDGLREILGASIAESEDSGYWMTLFRSLKDRGLDGV
                     ELVISDAHKGIQKAVESSFVGASWQYCHVHFFRAVLDSIPNKDKKEIADKLKEASDDE
                     MEMQELAKELRDRGYDPAAETIDRFRFDLWNYKAYPKAHWKRIHTTNIVERINKEFKR
                     RSRPVGAFPSDQSLMRIAGCIMININEEWITGKKYLTMDEE"
BASE COUNT          869 a          566 c          681 g          638 t
ORIGIN      
        1 ccctgggctg cagcacatgc acatagtgcc gcaagcgata taataaagac tgcagacaca
       61 cgagatactc ccgccgcctt cacagatgac atcgatcccg aagatgatat ttccctcaag
      121 tcactcacct caattctcct aaatcccaaa tcaagcgaat gataagctgc ttgttttcgt
      181 cacttgtcga agtgacgcgc tcaatgaata taaatattat gcattcactc ctgttgtgta
      241 tagtcccgaa acctttgact ttatataccc ttcaatgggg catggggaag agaaataagg
      301 tcgtaaagct aacagagagg aagatccaat acatcattcg ggccaaaaag agaggcgaga
      361 gcaccaaaag aatcgctgta gacatgaaac tcagcgaatc aacggtgaag cgagtatgga
      421 tgcactagtt gcgaaccaag atgcccctaa acattagaaa attcggaaga aagaaaaagg
      481 agttggaccg cgattccgtg cagctgatac ttgaagtcaa caaagagcaa aatcttggcg
      541 caaggcgtct ggaaaagatc atcgaattca agtacggaaa gcatatacct cataatgcca
      601 tccatagagt tctattggct catggtcgag ccaatgtgaa ccgcaacaag gcgaagcgca
      661 gaaagccctg ggtcagatgg gaacgagatc acagcttgac cctggtgcat ctggattggc
      721 atgacagtga catcaatggg aagaaggttt gcgccgtcct cgacgacagc tcacggcgca
      781 tccttgcagg cggtgaattc gatgaagaga cctcggataa cagcatccgt ctcctccagg
      841 aggctctcga taatttcgag tggctgacgt ccatcagcca ggtaatgaca gatcacggga
      901 ctcagttcta tgccaacaag agagacaaaa atggtaacgc cgattgcgga tttgagaact
      961 tcctcaaaag aaataaaatt aagcacatat tagctccagt caaacgcccg caagtcaatg
     1021 gcaaatttga aaaatggaat gatacttacg agaaaaacag aattaggttt gataattttg
     1081 acgaattcat gaattggtac aacacggtca ggttccatga atcactcgat acgaaatggt
     1141 atctgcagac gccagatatg gcattttggg caaggctgcc tgaagggtgt aagctgggaa
     1201 tgttcttgaa atacatggag actgaattaa atggtggaaa ctgaatataa cttccggtcc
     1261 ggagtatata aagtcaagac attcgggact atacagtgtt cgattcagag gcggaaagtc
     1321 tccccatttc atgttcttat cccatagcaa aacattctaa tgggcagtgt ctcgatcttg
     1381 atgtaaaata gtagtaataa tcctggcgat gtatagtatg ctactatgcc gataggagca
     1441 ttctgatcat ccgcaggatt tataataagc cctgtatcca gacttgagtt atcgaaaaac
     1501 caaggaggat acaaggccat gcaatcatta gagccggtaa taaattatct tgtcgatccg
     1561 aatagcggac ttagtcaggt cataaccgag ttcctgaacg cggttatgca gtatgaggca
     1621 tatctgcaga gcggtgcact cccttatgag cgctgccctc agagaaaagc gcatcgaaac
     1681 ggcactagac caagatccct caagacccgc gttggcgaaa tagtccttga taagcctcag
     1741 ttcagagaaa tcccatttga atcgagtata tttgaaaggt attgtcgcgt ggagcaggct
     1801 ctgctgatga caatcgcaga atcgtatatt caaggcgttt caacccgtaa aatggaggaa
     1861 ttagtcaaaa gcttcggttt gaatagatta tccgcatctg aagtctctcg gctatgcaag
     1921 attctagacg aaaaggtcga agagtttctt aagcgcccga ttgaagttaa gattcgctat
     1981 ctcgtggtcg atgctaccta tttcaaggtg agatgtggtg tccaatataa aaacagggct
     2041 ctcctgattg tagtagggat tcgcgaggac ggcctcaggg agattttggg cgcgagcata
     2101 gcggaatcag aggatagcgg ctattggatg acgctattca gaagcctcaa agaccgggga
     2161 ttagatggtg tggaactggt gatatctgat gcgcacaagg gaattcaaaa agcagtggaa
     2221 tcatcgtttg ttggagcctc ctggcagtat tgccatgttc actttttcag ggcggtcctg
     2281 gacagcattc ccaacaagga caaaaaggag atcgccgata agctaaaaga agcatcagat
     2341 gacgagatgg agatgcagga actggcaaaa gaactaagag atcgggggta cgatcctgca
     2401 gctgagacaa tcgataggtt cagattcgat ctgtggaatt ataaggcata tccgaaggcg
     2461 cattggaaga ggatacacac cacaaacatc gttgagcgta tcaataagga attcaaacgc
     2521 agaagcaggc ctgtcggagc attcccgagc gaccaatcac tgatgagaat tgcaggatgt
     2581 attatgataa atataaacga agagtggatc acgggtaaaa agtatttaac gatggacgag
     2641 gagtaatgat attaggatac agggccgcta tgaaattaca gcaaattcgg cacactgcca
     2701 taacttttga caaatttcat gtaatgaagc ttatgaatga ggcagtcgat cagg
//
LOCUS       DAOOWY010000182         2736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_37079_length_2736_cov_10.853040, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000182 DAOOWY010000000
VERSION     DAOOWY010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37079_length_2736_cov_10.853040"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PK105_08880"
     CDS             <1..406
                     /locus_tag="PK105_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOU61871.1"
                     /translation="YTSGDLYTMERLISAFPDKIVFSGYDELLLCGKALGAFGAIGST
                     YNLFAHIAKEIWKNVDLGHLAQARAGQAVLNSAIQELTTLGLYQALKEIISISGIDTG
                     CCLPPFSPLSQEQKEKADALGRKYEDMKLIRL"
     gene            576..1364
                     /locus_tag="PK105_08885"
     CDS             576..1364
                     /locus_tag="PK105_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOU61872.1"
                     /translation="MAKVNSVRKALAILDLLSDGKKRSLSDIARELCLAKSTVSNLLE
                     TLTTERILERNRESGLYSLGIRLIELGYCTQTGYDLVRIAAPFLRGLNQEFDETVHLV
                     LLDRDEVLYIDCIESHRRLRTYSVIGVRAPLHCTSVGKAILAFLSDSEIRGIISRKGL
                     LSFTSHTITTEERLWNEITIIREHGYAIDDMEHEDHLRCVGAPIFNVEGKVFASVSIS
                     GPAERNTLKRLESMAPALLEATTEISHRLGYRNKDQSRAGSQII"
     gene            1385..2314
                     /gene="dapA"
                     /locus_tag="PK105_08890"
     CDS             1385..2314
                     /gene="dapA"
                     /locus_tag="PK105_08890"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOU61873.1"
                     /translation="MAEYEKERYGKILIPLLTSFCEDGRVDFERTAALAEFIIDQKKG
                     DSLVLAGSTGEFYAMSFDERVRLFETVKNKVGDRIPIIAGIGAASTIETIQLAKIAEK
                     IGFETVMIVSPYYTKPNQEELYCHFMKVATSVSLNILLYNIPIYTGVNIDPSTISRLA
                     EQPNIVGIKEEAELNPKQITAFLNCTPENFVIYNGDDTMILEAYAQGGEKRIGGVVSG
                     SSHIIGHLIRSMIENFLTGKVQAAAEMQQRIYPFLKSMGQNNRINAAPLLKEALRLYG
                     FDAGYPRAPLICGTPNEIENIQLAMMQLDLLRK"
     gene            2443..>2736
                     /locus_tag="PK105_08895"
     CDS             2443..>2736
                     /locus_tag="PK105_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU61874.1"
                     /translation="MKKTIVAMMVLLLLASSTAFAQQKPIKLVFTSVSVPTDAHTQAM
                     YVFEEEVERLSGGQIQVDVYDSGKLFTQQAEQDAIRKGTVDMVYSSASWLAEFV"
BASE COUNT          815 a          582 c          600 g          739 t
ORIGIN      
        1 atatacatct ggcgatctct ataccatgga acgacttatc tctgcttttc cagacaagat
       61 cgtattttct ggctacgatg agcttttgct ttgtggtaaa gcgcttggag cctttggcgc
      121 gataggttcg acatacaacc tgttcgcaca tatagctaaa gaaatctgga aaaatgtgga
      181 tcttggtcat cttgctcaag cgcgcgctgg ccaagccgta ctcaacagcg ccatccaaga
      241 gcttacaacc cttggcctct atcaagccct gaaggaaata atatccatat caggcattga
      301 cacaggatgc tgcctgccgc ccttctcgcc gctatcacaa gagcaaaaag aaaaagccga
      361 tgcgcttggc cggaaatacg aagatatgaa attgataagg ctttgagcaa tagttgtatt
      421 ttttctatat gcttggcatg cagttgtgca ccgaatttat tgtgtacaga aagttattct
      481 ttgcatgaat tcttcttgac aacatctaat tcccatgaga caattcatat ataagaacat
      541 tgttcttata tatgaacaat tctgtagaga tttgaatggc aaaagtaaat tctgttcgaa
      601 aagcactcgc aatcctggat cttctttcgg atggaaaaaa acggagcctg tcagacatcg
      661 cgcgcgagct atgtcttgca aaaagcacag tctccaacct gcttgaaaca cttactactg
      721 aaagaatact tgagcgaaat cgcgaatcag gcctgtattc gcttggcatt agacttattg
      781 aactgggtta ttgtacacag actgggtacg atcttgtgcg aattgcggct ccatttctgc
      841 gcggtctcaa ccaggaattt gatgaaacag tacaccttgt tttgctcgat cgtgatgaag
      901 tgctctatat cgattgtatc gaatcgcatc gccgacttcg aacctattct gttattggtg
      961 ttcgcgcgcc gctccattgc acatctgttg gcaaggctat tctcgctttt ttatcagatt
     1021 ccgaaatacg gggtattatc tcaaggaaag gcttgctgtc cttcacatcc catacaataa
     1081 cgaccgagga gcgcctctgg aacgaaatta caattatccg tgaacatggt tacgcaatcg
     1141 atgatatgga acacgaggat catctgcgct gcgtaggagc ccccatcttc aatgtggaag
     1201 gtaaggtctt tgcatcggtc agcatatcgg gtcctgccga aagaaatacg ctaaagcgac
     1261 ttgaatcgat ggctccagcc cttttggagg caacaacgga gatatcgcat cgtcttggat
     1321 atcgaaacaa ggatcaaagc cgcgcaggca gccaaataat ataatccgat ttaaggaggt
     1381 ttttatggca gaatatgaaa aagaacgcta tggaaagata ttgatacctc tgcttacatc
     1441 cttctgcgaa gatggcagag tagactttga aagaaccgct gctcttgccg aattcattat
     1501 tgatcaaaag aaaggtgata gccttgtcct cgcaggatca acaggcgaat tttatgctat
     1561 gtcgtttgac gaaagggtaa ggttattcga aacagtcaaa aacaaagtcg gtgatcgaat
     1621 tcccataata gcgggcatcg gagcagcttc tacaatcgaa acaattcagt tggcaaaaat
     1681 agccgaaaaa atcggttttg aaacggttat gattgtatct ccatattata caaaaccaaa
     1741 tcaagaggag ctatattgcc atttcatgaa ggtagcgaca tcggtatctc tcaacatact
     1801 cctatacaac attcctatct atacgggtgt taatattgat ccatctacaa taagccgtct
     1861 tgcagaacag ccaaatatag tcggaatcaa agaggaagct gagctgaatc caaaacagat
     1921 cacggctttt ttgaattgca cacctgaaaa ttttgtcatc tacaatggcg atgatactat
     1981 gatccttgaa gcttatgccc agggtggcga aaaacgcata ggtggcgttg tttcaggatc
     2041 atctcatatc ataggacatc ttataagatc tatgatcgaa aattttctta cagggaaagt
     2101 ccaagcagca gcagaaatgc agcagagaat ctatccattc ctgaagagca tggggcagaa
     2161 taataggatt aatgctgctc ccctcttgaa agaagcgctt cgactttatg gattcgatgc
     2221 tggatatcct cgagcaccac tcatatgcgg aactccaaat gagattgaaa atatccaatt
     2281 ggcaatgatg caactagatc tcttgaggaa atgaagaaac cagacctaaa catcgccaat
     2341 tggactgata aaggcaatat ctattgccgt atagataata gctaggcatt tagccgtctt
     2401 ttgtacggct ggagatagag gatgcatata aggaggtatt ttatgaagaa aaccattgtt
     2461 gcaatgatgg tattgctcct acttgcaagc tcaacagcct tcgcccaaca aaaacctatt
     2521 aagcttgtct ttacttcagt cagtgtgcca acagatgcgc atacacaggc aatgtacgtt
     2581 ttcgaggagg aagttgagag actttctggc ggacaaatcc aggtcgatgt ctacgactct
     2641 ggcaagctct ttacccagca ggccgagcag gatgctattc gtaagggaac agtcgatatg
     2701 gtctatagtt ctgcatcgtg gcttgcagaa tttgtt
//
LOCUS       DAOOWY010000183         2706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_37610_length_2706_cov_9.858167, whole genome shotgun sequence.
ACCESSION   DAOOWY010000183 DAOOWY010000000
VERSION     DAOOWY010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37610_length_2706_cov_9.858167"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..319
                     /gene="tnpB"
                     /locus_tag="PK105_08900"
     CDS             152..319
                     /gene="tnpB"
                     /locus_tag="PK105_08900"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HOU61875.1"
                     /translation="MHLKKNRKIYLVMGFTDMRKQINGLAQIAEAKKPKKLLSGNYFL
                     FYQRWREWFYL"
     gene            complement(573..1724)
                     /locus_tag="PK105_08905"
     CDS             complement(573..1724)
                     /locus_tag="PK105_08905"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU61876.1"
                     /translation="MSLLKKKILILMMEVGMGHKIPALAIKEELERKYPDRFDISVLD
                     FARECGALGVDRQLKKGWDIALSFPFSVRIGYRFIELIKNNFFYIELLCRDFIKKGMD
                     YIANTKPDAVLATHTLCLYIACKARRTFGGNYKVLCCVVDPFDGYSLWANLDTDLFFV
                     ATEQSKKRLIDHGVNASKILVTGFPMRKSFFVFMKNKDNIVHELGLDPAKCTILISAG
                     SRGISNVYSFAECVKKKGIPFNIIAVTGKNQHLKEHLDTLASIKNETNLVSLGYVDTM
                     NELMAASDVIAGKAGASTFMEALFLKKPMIFTGWTAYNDWYIIDFALKHNIGWYCPTL
                     RSFLTIISKLSSEPHMVHEYSERIEALHFTSGTETIADIVACEFGNERL"
     gene            complement(2485..>2706)
                     /locus_tag="PK105_08910"
     CDS             complement(2485..>2706)
                     /locus_tag="PK105_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HOU61877.1"
                     /translation="TRLEVILKLPNRAAVAVRQAFDQLERQLGSELFRTMFRSIALDN
                     GVEFSLVYDLERAVLTKDTRTTLYFAHTL"
BASE COUNT          781 a          584 c          617 g          724 t
ORIGIN      
        1 ttttcaaaga agtcgttgga gctccctctt atgttatgct atctgataaa gaaaccttgc
       61 ttgcaaggaa aacttcgtaa ccatcaaacg cacactgtcc ctcaaataga aaaaatctga
      121 ccatcctatc ttccaaaagg aatacaggag gatgcacctc aagaagaatc gcaagatcta
      181 tttagtcatg ggtttcactg atatgcgaaa gcagatcaat ggccttgccc aaattgcgga
      241 agcgaagaaa cccaagaaac tgctctcggg gaactacttc ctcttttatc agagatggag
      301 agaatggttt tatctctgaa aacgacatag gagtttttgt acaaactgtt gttagcgcgc
      361 ttttcgagag agaaaagttt gaaagaatcg gagcagaggc acagcgcaca ctgtgccgca
      421 cctgggagag cattatggaa gacattgcca cgaggtatcg aactatttta gagaaatagc
      481 ttcgaaaatg aggctcgtgc catcgagaaa gagattagaa ttgaaataga aatgagcaga
      541 catcgatatg ataaggagcg atgaagccct atctatagtc tctcgtttcc aaattcgcaa
      601 gcaacgatat ctgcgatggt ctctgtgcct gaggtaaaat ggagcgcctc gattcgctcg
      661 ctatactcgt gcaccatatg aggctcgctc gagagcttgc tgataatggt gagaaaagat
      721 cgaagagtgg ggcagtacca tccaatattg tgtttcaatg caaagtcgat gatgtaccag
      781 tcattgtagg cggtccatcc cgtgaaaatc atcggctttt tcagaaagag cgcttccatg
      841 aaggtcgatg cgcctgcctt tcctgcgatg acatcggaag ccgccataag ttcgttcatg
      901 gtatcaacgt atccaagaga aaccaggttt gtctcatttt tgatggatgc aagagtatca
      961 aggtgttcct tgaggtgctg atttttccct gtgacagcaa tgatattgaa aggaataccc
     1021 ttctttttta cgcactctgc aaacgaatag acatttgaaa tacctcgaga accagcagaa
     1081 atgaggattg tacactttgc agggtcgaga ccaagttcat gaacaatatt gtctttgttt
     1141 ttcatgaata caaagaagct cttgcgcatt gggaatcctg tgacgagtat cttcgatgca
     1201 tttacgccat gatcgataag gcgttttttc gattgttcag tcgcgacgaa aaagagatcg
     1261 gtgtcaaggt ttgcccagag actgtaacca tcaaagggat cgaccacaca gcacaaaacc
     1321 ttgtaattcc cgccaaaggt gcgcctcgct ttacacgcga tatacagaca aagtgtgtga
     1381 gttgcaagga ctgcatcagg cttagtgttt gcaatataat ccattccctt cttgatgaaa
     1441 tctctacaga gaagctcgat ataaaagaaa ttatttttga tcaactcaat aaagcgatat
     1501 cctattcgaa cgctaaaagg aaaagagaga gcgatatccc atcctttctt aagttggcgg
     1561 tctactccta gggcaccgca ctctctcgca aagtcgagaa cgctgatatc aaatctatcc
     1621 ggatattttc tttccaattc ttcctttatt gcgagggcag gaattttatg gcccattcca
     1681 acttccatca tcaatatcag aattttcttt ttcaagagag acatgagagc ccccattttt
     1741 tctggatcaa taggcaccta ttgcgataat gatgccgcca ttgacagcta gaagataatt
     1801 catcacatgt caaaagggaa tagataggac tgatcgtctt ctctttgaga ttatcttgat
     1861 gattgctttg agattcttcg attcagcaac aaaatcgaga tcatcgccag gcacgatcaa
     1921 cacagggtgc caagaaaaat gaagataccc attcctcata gagggcatcg agcccttcga
     1981 gatagctatc agagatcgac ggctcaaact ctctttcctt ctgagatgga tcgatcttgg
     2041 actactgagt gaggatcctc gctgagcccg cgatgcatct gggctcgata cggcaaaaaa
     2101 caatataaaa tctgacttta ctatggaggt ccatgcgggc gaatatagaa tttatctatt
     2161 tccttctgtg cccacgccta ggtaggctac actgactttt gggttggaag agagttctga
     2221 ggtattgcct tgcaaatcaa ctttgcccaa ttcgagaatg tatcctctgg aagccagctt
     2281 cagggctttt cgagcgtttt gctcaacgct aaggatggct acttttcctc ttggtgaatt
     2341 ttctggatca tatcgatcat atcgaagatc ctattgatga ggattggcgc aaggccaaga
     2401 gaaggttcgt cgaggaggag taagccagct gctgacacaa gtctcctgta aagagcaatg
     2461 tcgactaaat tgattcttag gtttttataa ggtatgagcg aaatacagag tcgtccgtgt
     2521 gtctttggtc aacaccgcgc gttcaaggtc gtagacaagg gagaattcga caccattgtc
     2581 cagggcaatg gagcgaaaca ttgtccgaaa aagctcgctg cccagctgcc gttcaagctg
     2641 gtcaaaggcc tgtctaaccg caaccgcggc cctatttgga agtttaagta tcacttcaag
     2701 ccgggt
//
LOCUS       DAOOWY010000184         2682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_38054_length_2682_cov_10.708413, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000184 DAOOWY010000000
VERSION     DAOOWY010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38054_length_2682_cov_10.708413"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1241
                     /locus_tag="PK105_08915"
     CDS             261..1241
                     /locus_tag="PK105_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOU61878.1"
                     /translation="MKKLSLLVLTVVLLGLVATVGAAPAWKPTKTIELVAPANPGGGW
                     DMVCRVIQKALTEEKLVDKPVIITNKPGGGGSVGWTYLKGKKGQGEYLAATSSLLILN
                     NLLGTSDLTYKDFTPIACMQAEWEAIVVREDSPWKTVNDLMEAIKANPGAVPIAVGPS
                     LGNDDHLVFLQLARTYGVNPAKVKFIVYPGAGGEEVPALLGGHVKAMVIGLAEVLEQH
                     RAKKLRVIGVSSPERLELLPDVATIKEQGIDIVFPHWRGIIGPPGLTPEQVAYWDEVF
                     TKMVQTKTWKEQLENLLWDDYYQNSSDHAKFLEEQTKVFDELLTAVGLKK"
     gene            1330..1806
                     /locus_tag="PK105_08920"
     CDS             1330..1806
                     /locus_tag="PK105_08920"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOU61879.1"
                     /translation="MTTNILTGIISIVLGLVYSITALRLPRMAMGDRLGPKIFPYFVG
                     IVTIITGIALILQDRNPEKRSKKAEFGFKEHKNLWLNIVILTIAGILFGFFIDELGYM
                     IATALFIFSVSMLINKGKLVQNIVIAVLFPVVTYFVFGVALKLSLPRGIIETMLPF"
     gene            1818..>2682
                     /locus_tag="PK105_08925"
     CDS             1818..>2682
                     /locus_tag="PK105_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOU61880.1"
                     /translation="MQSILTNLSIGLQTAFTLTNILWVFIGGTLGTIIGMLPGLGPAT
                     GVAILIPITFGMNPATALITMAAVYYGAMFGGSRASIMLNIPGDASAIVSCFDGYPMT
                     KKGKAGTALAISAIASFIGGTIGMIFLIFLSQPVASAALKFGPAEMFSLMAFALVATI
                     TLSEGNLLKGLFSIVVGLMLSTIGIDPLAGVVRFTYGVSSLNEGIDFLVIIIGVYAVT
                     EVYKNLKSIDRVYDFDSKSIGKVKVSKEEFKQCIVPMLRSTPLGFIIGVLPGMGGTIA
                     TFMSYAMEKNLS"
BASE COUNT          730 a          601 c          630 g          721 t
ORIGIN      
        1 tcttgcgcat ggtcttctcc aaccgcatag acaaaagtat ccaccgcaga aatgagcttt
       61 ataaggcctt ccttgcgcat gatcctgacc ttgcttgtcg gatatggaca gatcatctta
      121 acgagtcctt cctgatctgg cgcgaaaaat caggcaattc ggaagtgttg aaggattttc
      181 aattcttttg aagatttaac cgaccaggcc attggccgac ggtagatcat aaatttcaag
      241 tttttaagga ggagaccgaa atgaaaaaat tgtctctatt agtgctgact gttgttcttc
      301 tgggacttgt cgcaacagtt ggcgctgcac ctgcttggaa gccgactaag actatcgagc
      361 tcgttgctcc ggccaacccg ggtggcggat gggatatggt ctgtcgcgta attcagaaag
      421 cgctcaccga ggaaaaactc gtcgataagc cggtaatcat tacgaataag ccgggcggtg
      481 gtgggtccgt tggttggact tatctaaaag gcaaaaaagg ccagggcgaa tatctagccg
      541 cgacctcgag tttgctcatt ttaaacaatc ttctgggcac aagcgatcta acatataagg
      601 acttcactcc aatcgcctgc atgcaagcgg aatgggaggc catcgtagtg cgggaggact
      661 ccccctggaa aacggtaaat gatctcatgg aagcgataaa agccaatcct ggcgcggttc
      721 ctatagccgt cggtccttcg ctgggcaacg acgatcacct tgttttcctg cagcttgcaa
      781 gaacctatgg tgtcaacccc gcaaaagtaa aattcatcgt atatccgggt gctggcggcg
      841 aggaagtccc agccctactt gggggccatg taaaagccat ggtcattggc cttgcggaag
      901 tgctcgaaca gcatagagcc aagaaactcc gcgtaatagg agtgagttcg cccgaaagat
      961 tggaactttt gcccgatgtg gcaacgataa aagaacaggg aatcgacatt gtatttccac
     1021 actggagagg cattataggc cctccaggat tgactcctga acaagttgcc tattgggatg
     1081 aagtgttcac aaagatggta cagacaaaaa cctggaaaga gcaactcgag aacttgcttt
     1141 gggatgatta ctaccagaat tcctcagacc atgccaaatt cctagaagaa caaacaaagg
     1201 ttttcgatga gctattgacc gcagtaggtt tgaagaaata atccaacaat tatgaagagc
     1261 cggtgctatg ccaagactcg catgcgccgg cctttttatt agacataatt tctagggcgg
     1321 aggtcttgaa tgacaacaaa tattcttacg ggaatcatca gcattgttct aggcttggtg
     1381 tattccataa cggccttgcg cctgcctcga atggctatgg gagacaggct aggccctaaa
     1441 atattccctt atttcgtcgg tatagttacg ataataaccg gcattgcctt gattttgcag
     1501 gatagaaatc cagaaaagcg ctcgaagaaa gccgaatttg gttttaaaga acataaaaat
     1561 ctatggttaa acatagtcat tttgacaatt gctggcatcc tctttggatt tttcatagat
     1621 gaattgggct atatgatagc tactgcgttg ttcatcttct cagtttcaat gctgataaac
     1681 aaaggaaagt tggtacaaaa catagtgatc gccgtccttt ttcccgtggt gacttacttt
     1741 gtatttgggg ttgcacttaa actgagcctt cctcgcggta tcattgaaac gatgcttcca
     1801 ttttaggaga tacacccatg caatccattt taacaaatct atctatcggg cttcagaccg
     1861 cctttacact tacaaatatt ctttgggttt tcatcggagg cacattgggc accataatag
     1921 gcatgctacc cggcttagga cctgcaacag gtgtagctat tctaataccc attacctttg
     1981 gcatgaaccc agccacggct ctcataacca tggcagcggt atattatgga gctatgttcg
     2041 gaggctccag ggcatcgatt atgttgaaca tacctggaga tgcttccgct attgtctctt
     2101 gcttcgatgg ttatccaatg accaaaaaag gcaaagccgg tacagcgctt gccatctccg
     2161 caatcgcttc gttcatcggt ggaacgattg gcatgatatt tctcattttt cttagccaac
     2221 cagttgcttc cgcggcgcta aagtttggac cagcggagat gttctcgctc atggcctttg
     2281 ccctggtggc aaccatcacc ttatcagaag ggaacctttt gaaaggactc ttttcgatag
     2341 tggtagggct tatgctctcg accataggaa tagatccact tgctggagtc gtgcgtttta
     2401 cctatggagt ttcgagtctt aatgaaggta tcgattttct tgtaatcatc atcggagtct
     2461 acgccgtcac cgaagtatat aaaaacttga aatcaatcga tagagtatat gactttgatt
     2521 ccaaaagcat tggcaaggta aaagtgagca aggaagaatt caaacaatgc attgtgccta
     2581 tgctccgaag tacaccacta ggatttataa tcggagttct tcctggcatg ggcggtacaa
     2641 tcgccacatt catgtcctat gccatggaaa agaatcttag ca
//
LOCUS       DAOOWY010000185         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_38235_length_2672_cov_12.560183, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000185 DAOOWY010000000
VERSION     DAOOWY010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38235_length_2672_cov_12.560183"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..767
                     /locus_tag="PK105_08930"
     CDS             279..767
                     /locus_tag="PK105_08930"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOU61881.1"
                     /translation="MPVWLKKTCKFLVDCIELYIPILSFSTLFVSFVLQIFARKFFKP
                     LLWPEELSLICFVWTALLGGLYSKRMGTQVAFTMLYDAAKPKTKRIMRIVGNALLLVA
                     FLIILVPSWDYVQFMQYKKSDALGIPMNIAYFPFVVYLVDMIIRLSIDIAKDFGTLRG
                     TK"
     gene            764..2062
                     /locus_tag="PK105_08935"
     CDS             764..2062
                     /locus_tag="PK105_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU61882.1"
                     /translation="MNLPLIVFILSFVAVFLLGIPLAPGMMLSSAFYFLLSSNPAASL
                     DMVAMQFLTNLNASFVLIAVPLFVFMAEIMNSGKVTEMIFSFSSALVGKRKGALAHVN
                     IINSIIFSGMTGSALADASGPGNMELQAMKQQGYDDAYTCAITAASATIGPIIPPSIP
                     MVVYAMLSSTSIGYLFLGGIIPGLLVGIALLIYNAFISNKRNYPQGKQFPARQFWKFA
                     LQALPALFTVVILLGGIYTGVVTPTEAGALAAFYGILISVSVYRALGWKEFKQCLINA
                     VRTTGTLSLLVGSSFAFSYIVSIEQIPNVIATFLLGLTQNKYVMLVILNIALLILGCF
                     LDTTPIQLIFTPIIIPILSQLGIDLVHFGVVMVLNLMIGLSTPPYGMLLFTVSGLTGT
                     PLKDIIREILPMVLIIIIVLFLITYIPDLVLFIPKLSGYK"
     gene            2070..>2672
                     /locus_tag="PK105_08940"
     CDS             2070..>2672
                     /locus_tag="PK105_08940"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOU61883.1"
                     /translation="MKQTIPDGVYPTLITPYEEDGSIDWNALEALIEWHIARGVAGFF
                     AVCQSSEMFHLTLRERLELARMSIKFAQGRVPVLVSGHVADTLYDQIEEAKMMADTGA
                     DALILVTNRLAGRYESDDILKRNLEAFLTAFQNDILLGFYECPAPYKRLLNADLVQFI
                     VKTRRFGFLKDTSCRVGDMQAKIEAAKGSNFKLFNANAATL"
BASE COUNT          693 a          607 c          573 g          799 t
ORIGIN      
        1 cgaaaaggaa atcctggatt tcttccgcca gcaaggtctt gttgtcatag aaaatcctga
       61 tcgcgatgca tttgcagctt acgcaaagaa tttctatctc acagaaggca aagatatctc
      121 aaaagattgg gacctcactc tctatgatga aatacaaaaa ctgaaataat acctagagct
      181 tgcctgcagc agagatctgc tgcaggcagc cttgattcaa taagtatgtc gatatcgtat
      241 cgatgctttt tgaaagggta tttgaaggag gaggcattat gccggtttgg ctgaaaaaga
      301 catgcaaatt tcttgtagac tgcattgagc tctatatacc tattctcagt tttagtacgc
      361 ttttcgtctc tttcgttctg cagatattcg cacgtaaatt tttcaagccg cttttatggc
      421 cagaagaatt gagtttgatt tgctttgtat ggactgctct actgggtggt ctttactcga
      481 agagaatggg cactcaggtc gcattcacaa tgctctatga tgctgccaaa ccaaagacaa
      541 agcgcattat gcgcattgtg ggcaatgctc tgcttcttgt agctttttta atcatcctag
      601 ttccttcatg ggattatgtg cagttcatgc aatataagaa atcagatgca ctgggcattc
      661 ccatgaatat cgcgtatttc ccatttgtcg tgtacctagt cgatatgatt atcaggttga
      721 gcattgatat cgcaaaagat ttcggcacgc tcagagggac taaatgaacc tcccacttat
      781 tgttttcata ttgtcgtttg tagccgtatt cctgcttggt attccccttg cgccaggcat
      841 gatgttgtct agcgcatttt attttctcct ttcgagcaac cctgctgcgt cgctagatat
      901 ggtcgctatg cagtttctta ccaacttgaa tgcgagcttt gtcctgattg cggtaccact
      961 ttttgtcttc atggccgaga taatgaactc aggcaaagta actgaaatga tcttctcatt
     1021 ttctagcgcg ctcgtgggta agcgaaaagg cgctcttgca cacgtcaaca ttatcaactc
     1081 gatcattttc tctggaatga caggttcagc gcttgccgat gcctcaggtc caggcaatat
     1141 ggaattacag gcaatgaaac aacaaggcta tgatgatgct tatacctgtg ccatcaccgc
     1201 agctagcgcg accattggac caattatccc gccgagcatc cctatggttg tatatgctat
     1261 gctctccagt acatcgatag gatacctatt cctgggaggg ataattccag gactactcgt
     1321 tggcatagct ctcctgatct acaacgcatt tatatcgaat aaaaggaatt atcctcaagg
     1381 aaaacaattt ccagctaggc aattctggaa atttgctttg caagctttgc ctgcactttt
     1441 caccgtagta attttgctgg gaggaatata tacgggcgtt gttactccca cagaagcagg
     1501 ggctttggca gccttctatg gaatactcat atccgtgtcc gtataccgag cgcttggttg
     1561 gaaggaattc aagcaatgcc ttatcaatgc ggtgcgcacc acaggaacgc tttcgcttct
     1621 tgtcggaagc tcctttgcct tttcttatat cgtcagtatc gaacaaatac ccaatgttat
     1681 cgccacattc ttacttgggt taacccagaa taaatatgtc atgctcgtaa tcctcaacat
     1741 cgccttgctt attctgggtt gctttctaga taccacgccc atacaactca tattcacccc
     1801 tatcataatc cctattctca gccagttagg gatagacctc gtccattttg gcgttgtcat
     1861 ggtgctaaat ctcatgattg gactttcgac tccaccatat ggcatgctac tctttacggt
     1921 atcgggactt acaggaactc cgctaaagga tataatccga gaaatattgc ctatggtttt
     1981 gattatcatt attgttttgt tcttaataac ctatatccct gatcttgtac tttttattcc
     2041 caagttatca gggtataaat gaggccacaa tgaaacagac tattcccgac ggtgtatatc
     2101 caacccttat tacgccctat gaggaagatg gtagcatcga ctggaatgca cttgaggcgc
     2161 tcattgagtg gcatattgca cgcggagtag caggattctt tgcagtctgt caatcgagcg
     2221 agatgtttca tctgacgctg cgtgaacgac tcgaattggc aagaatgtcg atcaagtttg
     2281 cgcaaggtcg agtgcctgtc cttgtctctg ggcatgtagc cgatacactc tatgatcaaa
     2341 tcgaagaagc caagatgatg gccgatacag gcgctgatgc attgattcta gtgacaaatc
     2401 gcctggcagg ccgctatgaa agtgatgata ttctcaagag aaatctcgag gcattcttga
     2461 ccgccttcca aaacgacatc ctccttggct tctatgagtg ccctgctcca tacaaacgac
     2521 ttctaaatgc ggatcttgtg caattcattg tcaaaacaag gcgatttggt ttcttgaaag
     2581 atacaagctg tcgcgttgga gatatgcagg ccaaaattga agcggcaaaa ggcagcaact
     2641 tcaagctttt caatgcaaat gctgcgactc tg
//
LOCUS       DAOOWY010000186         2670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_38261_length_2670_cov_24.077629, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000186 DAOOWY010000000
VERSION     DAOOWY010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38261_length_2670_cov_24.077629"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2670)
                     /locus_tag="PK105_08945"
     CDS             complement(<1..>2670)
                     /locus_tag="PK105_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61884.1"
                     /translation="RLLPRILPPADQRIQNFIDQYLADLGLARIPQIPPNTLVLDHCG
                     MARELSLPPDEHKHASPTLTSYRVRNGVLHNPKSDRRTTEGVFHIAEGGLPIPLDKKA
                     VPKLCFAKLLEAALNPPLEIMELPFTAKENEKAHTFVSVLLRPIVRPEIPEYCEERSM
                     EIRFFAPGSLVANIDFVESIFGNSGDPFIPENDAAIDPVHWTGTTGCIILATHLTELT
                     KKEIGLPPWEQATERQRRDGMCWREPTEKYNDGKPFKIAARDESGIIITIIADNYFGY
                     SKKEIKSHISYSANLLGLAEEEHAGGALVFPSYNLGTRFVPDTNLKSKGHNLNDVYSI
                     MRSRIDMRQEGYGIDLTFPNIIYLPEDAYISLEDQMAHWVNDGIEESLRILPGNVYIN
                     PTGYQIRMEQHSTSGAWRLIGTSAEGLLCHKPCTVSGGGKSEIAKSIQDAIVYMPIVI
                     ADFDKDMQEARKIIEKDYVNRFRDQSENLGKDTRPILSPKRSLGSVIKLLSPSPAYTD
                     EYNEWLRSIPERIKSLVFLVKRFYRPDWGLDWMSHFSVDAVNGTTGNILKFEGKPIMG
                     SYLRVGKNEKGLNRFFKLRQDFMPAFKLQWEDDITASIIVPVNQLENLPDWASKHLSL
                     KFAKNCEARFFQRPDDAIIRGYDKQTEKDLARYDNFLSNFEPLTRADASRIIENTIHF
                     SEYTEPMRKFIEESEKDPDFEYFVASNCFRLVDGVPSKNPRYLQLDPNYTDPMARYLA
                     ELSPRLYRRIPADEPLPQPIGAVLPGRRNNPPDRQAGIRSLAVYGPIHYQELPELFMD
                     FVCSLTGKSPSTTGAGSEGALTKGPFNALVPTTDLNNALLGFILTGYAGFTTAAGYIG
                     HKYKVDHDISLMMPELWSRLSPEERDPEFLKKN"
BASE COUNT          634 a          581 c          719 g          736 t
ORIGIN      
        1 cattcttttt taaaaattct gggtcgcgct cttctgggct cagccgagac cacagctcag
       61 gcatcataag agatatatca tgatctactt tgtatttatg accgatatag ccggccgcag
      121 tagtaaaacc cgcatagccg gtaagaatga agcccaagag ggcgttattg agatcggtgg
      181 taggcacaag ggcgttgaag ggtccttttg taagagctcc ctcgcttccc gccccagtgg
      241 tggacgggct cttaccggtt agagagcata cgaaatccat gaatagctcc ggtaattcct
      301 ggtaatgaat cggaccatat acagcgaggg atcgtatacc tgcttgccga tcgggtgggt
      361 tgttcctgcg tcctggcaag acagcgccaa ttggttgagg aagaggttca tcagcaggga
      421 tgcgcctata tagccttgga ctaagctctg cgagatatcg tgccattgga tccgtataat
      481 ttggatctaa ttgcagataa cgcggattct tggaaggcac gccatcaacg agtctaaaac
      541 aattcgaggc tacaaagtat tcgaagtctg ggtctttttc gctctcttct atgaattttc
      601 gcatgggttc ggtgtattcg gagaaatgta ttgtgttttc gataatgcga gaggcgtcgg
      661 cgcgggtgag aggttcaaaa ttcgacagga aattgtcgta tcgcgctaga tctttttcgg
      721 tctgtttgtc atagcctcga ataatggcat cgtcaggccg ttgaaagaat cgcgcttcgc
      781 aattcttggc aaatttcagg ctcagatgtt tggaggccca atcaggcaaa ttttccagtt
      841 ggttgacagg cacgattatc gaagcagtaa tatcatcttc ccattgaagt ttgaaggctg
      901 gcatgaagtc ctgcctaagc ttgaagaagc ggtttaggcc tttttcattt tttcctacgc
      961 ggagatagga tcccattatg ggtttgcctt cgaatttcaa gatatttcct gtagtaccat
     1021 tgaccgcatc caccgagaaa tgcgacatcc agtcgagacc ccaatcgggc ctgtagaatc
     1081 gctttactaa aaacacgagg ctctttatgc gttccggaat ggaacgcaac cattcattgt
     1141 attcgtcggt atatgcaggc gaaggggaga gtagctttat gaccgaaccg agggagcgct
     1201 ttggagagag tatcggcctt gtgtctttcc caagattctc cgattgatcg cggaaacgat
     1261 tgacatagtc tttttcgatt atttttctgg cttcctgcat atccttatcg aaatccgcaa
     1321 tgactatagg catgtacacg atagcatcct ggatcgattt tgctatctca gacttgcctc
     1381 cgcccgatac cgtgcagggc ttatgacaga gaagtccttc tgcggaagtg ccaataagtc
     1441 gccatgcacc ggatgttgaa tgttgctcca tgcgaatctg atagcccgtt gggtttatgt
     1501 agacattgcc cggaagaata cgcagagatt cttcaatacc atcgttgacc caatgtgcca
     1561 tctggtcttc cagagaaata taggcatcct cgggtagata gatgatattt ggaaaggtaa
     1621 gatcgatgcc atagccttct tgtctcatgt cgatgcggct gcgcattatg gaataaacgt
     1681 cattgagatt gtggcctttg gattttagat tggtgtcggg cacgaagcgc gtgccaaggt
     1741 tataggaagg aaagacaaga gctcctcccg catgttcttc ttcggcgagg cctagaaggt
     1801 tagcagaata agagatatga gactttattt cttttttgga gtagccgaaa tagttgtccg
     1861 caattatcgt aattatgatc ccggattcat cgcgagcagc tattttgaag ggtttgccat
     1921 cattgtactt ttcggttggt tcgcgccagc acattccgtc acggcgttgg cgttcggtag
     1981 cttgctccca tgggggtagt ccaatctctt tctttgtaag ctcggtgagg tgcgtagcta
     2041 ggattatgca gcctgttgtg cctgtccagt gaactggatc tatggccgca tcgttttcgg
     2101 ggataaatgg atctccagaa tttccaaata tgctctcaac aaagtcgata ttggcgacta
     2161 gcgaaccagg ggcaaaaaag cggatctcca tcgatctctc ttcgcagtat tcgggaattt
     2221 caggacgaac gataggccgc aacaatactg atacgaaagt atgtgccttt tcgttctctt
     2281 ttgcagtaaa gggcagttcc ataatctcga gtggagggtt cagagcggcc tctaagagct
     2341 tagcaaagca gagcttaggc actgccttct tgtcgagtgg aattgggaga ccgccttccg
     2401 ctatatggaa gactccctcg gtggttcgtc gatcgctctt ggggttgtgg aggacgccgt
     2461 tgcggacacg gtacgaggtg agggtagggg acgcatgttt gtgctcatcg ggcgggagag
     2521 agagctcgcg agccatacca cagtgatcga gaaccagtgt gtttgggggt atctgcggaa
     2581 ttcgggcaag gcccaggtct gccaggtatt gatcgatgaa gttttggatg cgctgatcag
     2641 ctggtggaag gattctaggc aacagcctgt
//
LOCUS       DAOOWY010000187         2582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_39952_length_2582_cov_7.166601, whole genome shotgun sequence.
ACCESSION   DAOOWY010000187 DAOOWY010000000
VERSION     DAOOWY010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39952_length_2582_cov_7.166601"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PK105_08950"
     CDS             <1..969
                     /locus_tag="PK105_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61885.1"
                     /translation="CGHSFSSQTFSLDYFAKKRLDYRQIERLVSSSMSQRALSRHFKV
                     SLGTINNRIMRLSHQTLAMHSLLRPRASHREPVCIDGFVSFDRSQYFPNNITISLTSH
                     SQFILSLSHATLRRSGRCTDSQKRRKTILYRSVTFEKAALERSFSELLDQLYRERCTI
                     YSSPLLLITDEKCEYARALRVHPSRFSIHHLTVNSKVPRTFHNPLFASNYLDRELRKD
                     LADHRRETVCFPRNVANMLNRLVVYLGWHNYEKHYRIAKHIILTHAEVAGIERNAICK
                     ARESQFKERAFLSRIGLSLLERRLWLRSFSTPLKRKAEYVPFYAYA"
     gene            complement(1080..2270)
                     /locus_tag="PK105_08955"
     CDS             complement(1080..2270)
                     /locus_tag="PK105_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61886.1"
                     /translation="MHEKMLIDQALNAGKGIFRLEPNWVPRSFCIPGRRLKLHPNDYY
                     AYGPHRGGIDERWFASTTKADNGPLTTTYEGLSFIWVDETHHILLKDAIEIWGNEILG
                     DKIMKKYGGWRAYSKFFDNLGPLPFHLHHTDKMAAHIGQLGKPESYYFPAQLNNHGGA
                     FPYTFFGLNPGTTKDQIKACIADWEKRDNNILALSRAYLLQLGTGWDVPPGILHAPGS
                     LLTYEPQRASDVSAMFQNIVWNTYTAKELLQNNIPKDKRDELDYYIELLDWDLNTDPL
                     FFEHRFMEPKPVKPLEEMRGQGYEEYDIVYKSEYFSAKELTILPGRSVLIKDKGPYGA
                     IVIEGHGTFGNLSIESPAMIRFGQITQDELFVTDKAAKEGVLITNTSNTNALVMLKHF
                     GPAA"
     gene            complement(2300..>2582)
                     /locus_tag="PK105_08960"
     CDS             complement(2300..>2582)
                     /locus_tag="PK105_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOU61887.1"
                     /translation="LAHDERIIGAMILGAKHDLSFDAIAQVFNAALRFRATDEEGALF
                     PRDADFIAYISSHDIESILKEVCGLSLKDHVESIVYHRLSSLCEKIEHD"
BASE COUNT          669 a          618 c          549 g          746 t
ORIGIN      
        1 tgtggtcata gcttttcttc acaaacattc agtttagatt actttgccaa gaaaaggctc
       61 gactatcgac aaattgagcg tcttgtgtct tcgagcatga gccagcgcgc actctccaga
      121 cacttcaagg tctctttggg aactatcaat aatcgcatca tgagacttag ccaccaaact
      181 cttgccatgc attctctctt acgtcctcgt gcctctcaca gagaacctgt ctgtattgat
      241 ggctttgtca gttttgatcg ctctcagtac ttcccaaata atattaccat atcccttact
      301 agccatagcc agttcattct aagtctcagc catgccacat tacgccgctc aggccgctgc
      361 accgacagcc aaaagcgcag aaaaactata ctctatcgct cagtcacctt tgaaaaggct
      421 gctctcgaac gttctttctc agagctccta gatcagttat atagggaacg ctgtactatt
      481 tactcttctc cgctgctcct catcacagat gaaaagtgtg agtatgctcg tgcgctacgt
      541 gtacacccaa gtcgcttctc tatacatcat ctcaccgtaa attccaaagt cccacgcacc
      601 ttccacaatc ctctctttgc ctcaaactat ctcgacagag agcttagaaa ggatcttgcg
      661 gaccatcgtc gcgagacagt gtgctttccc aggaatgtcg cgaatatgtt gaatcgcctt
      721 gtcgtatact taggctggca caattatgaa aaacattaca ggattgctaa gcatatcatt
      781 ctgacgcatg ctgaagtagc tggtattgaa agaaatgcga tttgcaaggc tagagaatca
      841 cagttcaaag agcgtgcatt cctaagcaga ataggattgt cgcttcttga aagaaggcta
      901 tggcttagga gtttctcaac tccacttaag agaaaagctg agtatgtgcc tttctatgct
      961 tatgcatgaa gaacactcct agcagattgc taaagcaata tacacaaggt tcgtagcagt
     1021 attcttgcct tcctaatagg ctatcttttc aaggcctgcc tttttctttt ttgcctatat
     1081 catgccgctg gcccaaagtg cttaagcata acaagcgcat ttgtattgct tgtgttcgtg
     1141 atgagcacac cttccttagc ggctttgtct gtgacaaata gttcgtcctg cgttatttgt
     1201 ccaaagcgaa tcatcgccgg agattcgata gacaagttcc caaaggtgcc atggccttcg
     1261 atgacaatcg caccataggg ccccttatct ttgattagca cagaacggcc tggtaatatg
     1321 gtcagctctt ttgcggagaa gtattcgctc ttatagacga tatcgtattc ctcgtagccc
     1381 tgtccgcgca tttcttcaag gggctttaca ggctttggtt ccatgaatct atgctcaaag
     1441 aagagtgggt cagtgttgag atcccaatcg agaagctcga tataatagtc gagctcatct
     1501 cttttatctt taggaatatt attttgcagc aattccttcg cggtatatgt gttccacacg
     1561 atattttgaa acattgctga gacatccgac gctctctgag gttcataggt caaaagtgaa
     1621 ccaggagcat gcaaaatccc aggaggcaca tcccaccctg ttcctagctg aagcaaatag
     1681 gctcgcgaaa gtgcaaggat attgttatca cgcttttccc aatctgcaat gcaggctttt
     1741 atttgatcct tggttgtacc cgggttgagg ccaaagaaag tataggggaa ggcacctccg
     1801 tgattattga gctgtgcagg aaaataatag gactcaggtt tacctaattg tcctatgtga
     1861 gcagccatct tgtctgtatg gtgcagatga aagggcaaag ggcccagatt atcgaagaat
     1921 tttgaataag ctcgccatcc gccatatttt ttcatgatct tgtcgccaag gatttcattg
     1981 ccccaaattt caatagcatc cttaaggagt atatggtggg tttcatcgac ccagataaat
     2041 gagagacctt cgtaggttgt tgtaagaggc ccattgtccg cttttgttgt tgacgcaaac
     2101 catcgctcgt cgatgccacc tcggtgcggg ccataggcgt aatagtcatt tggatgcagc
     2161 ttgagccttc tgcctggaat gcagaaagag cgcggcaccc agtttggctc aagtctaaag
     2221 atgcccttgc ctgcattgag ggcttgatct atcaacattt tttcgtgcaa aattatagcc
     2281 tcctatcaaa atagcgaact caatcgtgct ctatcttttc acatagtgag gaaagccgat
     2341 gatacacaat gctttccaca tggtccttaa gagaaagccc gcatacttct ttgaggatac
     2401 tttctatatc atgtgatgat atgtatgcaa taaaatcggc atctcgcgga aacagtgcac
     2461 cttcttcgtc cgtagctctg aagcgaagtg ctgcgttgaa cacttgtgca attgcatcaa
     2521 atgataggtc gtgctttgct ccaaggatca tagccccaat aatcctttcg tcgtgcgcta
     2581 gc
//
LOCUS       DAOOWY010000188         2527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_41126_length_2527_cov_17.764968, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000188 DAOOWY010000000
VERSION     DAOOWY010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41126_length_2527_cov_17.764968"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PK105_08965"
     CDS             complement(<1..958)
                     /locus_tag="PK105_08965"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOU61888.1"
                     /translation="MWNYRKIDEAIPQNKYFLFDLAHDPEWSSPVIPSALGLTPDQRQ
                     RLQIQKEASGDSTPFIFIEGLAKEMTTWHFPHHFIDFETSVPALPFTRGHSPYETVAF
                     QFSMHNVYQDGSIRHAAQFLDATTGEHPNYKFLEHLRACLEWNDGTIFRYAAHENTVL
                     ANIRKSLLEDPDPPKERNYLIAFIESLICYEDGNGKRITGSRCMSDMFDLVKRYFYHP
                     MMKGSNSIKQVLPAIIASSRFLREKYSQPIYGTEKIPSLNIKNHIWIDERMDPYKTLP
                     PFFADLDPEELSQLSPSLEGFDEIHEGGAAMIAYCKLQFSEIP"
     gene            complement(1463..1894)
                     /locus_tag="PK105_08970"
     CDS             complement(1463..1894)
                     /locus_tag="PK105_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61889.1"
                     /translation="MGKYFTKSDFRMALECETQLYYAARKDEYADQHLDNSFLEALAD
                     GGFQIGALSKFLFVDDPVASKITCHDLSQDGGLAETATRMSQVNAIISEASFCYEDFF
                     ARADMIRKIESKLELYEVKAKSYNGNKSKFWKKSTHEISSE"
     gene            complement(2178..>2527)
                     /locus_tag="PK105_08975"
     CDS             complement(2178..>2527)
                     /locus_tag="PK105_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU61890.1"
                     /translation="LERMTEQRINFRSTLMDSAYDAKSIFQFVQDRGRTPIIDHNPRR
                     KPSREAFDEATKEHYKKRTVVERANSHLKDWLLPDKVLVRGSKKVSFELMYGVICLAA
                     IKILEKSILPSLI"
BASE COUNT          698 a          539 c          566 g          724 t
ORIGIN      
        1 caggaatctc gctaaattga agcttgcaat atgcaatcat cgccgctcct ccctcatgga
       61 tttcgtcaaa gccctcgagt gaaggcgaaa gttgggataa ctcctcagga tccaaatcgg
      121 caaaaaaggg cggaagagtc ttgtagggat ccatgcgctc atcaatccag atatggtttt
      181 tgatattgag gcttggaatc ttctctgtgc catagattgg ctgggaatat ttttctctaa
      241 ggaaccggct tgaggcaatg attgcaggaa ggacctgttt tatggagttt gaccctttca
      301 tcatgggatg ataaaaatac cgcttgacaa gatcaaacat atccgacatg catcgagacc
      361 ctgtgatcct tttgccattc ccgtcctcat aacagataag gctctcgata aaagctatga
      421 gataatttct ttccttagga ggatcagggt cttcgaggag gctctttcgt atatttgcta
      481 gaaccgtgtt ttcatgtgca gcatatcgaa atatcgtacc atcattccat tccaagcatg
      541 ccctgaggtg ttccaggaat ttgtaattgg gatgctcgcc ggtggtagca tctagaaact
      601 gtgcagcatg cctaatgctt ccgtcttgat agacattatg catgctgaac tggaaggcta
      661 ccgtctcata tggactatgt ccccgagtaa aggggagtgc tggtactgag gtctcaaaat
      721 cgatgaaatg gtggggaaag tgccatgttg tcatctcctt tgcaagacct tcaataaata
      781 tgaatggtgt cgaatcgcct gaggcctcct tttgaatctg aagcctttga cgctgatctg
      841 gtgtgagtcc taatgctgaa ggtataaccg gtgaagacca ttctggatcg tgcgcaaggt
      901 caaagaggaa atatttgttt tggggaattg cctcgtctat tttcctatag ttccacaaat
      961 ccaagatcaa ttgatggcca aaagcattgg gctttatacc gttctgttca tgccagcact
     1021 catgaaagcc cgattttaat gagtggacaa tgccagttcc ttccctatca tctcgaaatg
     1081 gacaacgctt gcatttgcta ccggtaggtg tagagatttt ttcgccttga agatggtgga
     1141 atgcgagaag atgaagatag tcatcaaagg aaacaatgcc aaatgcgggg gaagcgatag
     1201 gctgggtgag cagccattct acttgctcgg atacatcaac gggtgtaaca agaagagtgc
     1261 ctagatcggc ccgaacaagg ccatgtttga tttaaatcct ctcaattgaa ttctttccaa
     1321 gaatcttaaa acactggttg aggccataaa tatccgcttt ccttgtcctg tcggcaagga
     1381 ccaaaaatgg atgaatagtg agctctggat acctcttgcg aataacccat gtctggaaag
     1441 cagcatcata aagatagggt ttctattcgc tgcttatttc atgggtactc tttttccaaa
     1501 atttactttt gttgccatta taggatttgg ccttgacttc gtacaattcc aattttgatt
     1561 cgatttttct tatcatatct gcgcgagcaa agaaatcttc atagcaaaag gaagcctcag
     1621 agatgattgc attgacttgg gacattctcg tggcagtttc tgcaagtcca ccatcctgag
     1681 acaggtcgtg gcatgtgatt ttagatgcta caggatcatc gacaaaaaga aatttggaca
     1741 aggcaccaat ctgaaaccct ccatcagcca gcgcctcaag aaaggaattg tcgaggtgct
     1801 gatctgcata ctcgtctttc cttgctgcat aatagagctg tgtttcacat tcgagagcca
     1861 tgcgaaaatc cgattttgta aaatattttc ccatacggcc tgccatgtta ggaccgtgat
     1921 accgcaccca tattccctgc aaaatcatgc ctaaataatc ttataatctc gagcctcttt
     1981 caaaagtcca agaatattct gacttgaatt gtagtaataa gaaatagaga aaggattttg
     2041 caggataaat catggaacaa ggatcaacgg gtacaaaaga gtaagaaaaa gagccttaag
     2101 tgagcttttt acgaggtatg agcctatagc tctcttcgat gggtcatgag agcttttatg
     2161 atctaagata ttcgtcttta gatgaggctg ggtagaatag acttctcaag aatctttatt
     2221 gcagcaaggc agattactcc atacatgagc tcaaaactca ccttcttact ccctcgaacg
     2281 agcactttgt ccggcaagag ccaatcctta aggtgggaat tcgctcgctc taccactgtg
     2341 cgcttcttat agtgttcctt ggtcgcttca tcaaaagcct ctcgtgaagg ctttcttctt
     2401 ggattatgat cgatgattgg agtcctacct ctatcctgca caaactgaaa gatactcttc
     2461 gcatcatagg cgctgtccat aagagtagag cgaaagttga tcctttgttc agtcatacgc
     2521 tcaagag
//
LOCUS       DAOOWY010000189         2516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_41342_length_2516_cov_6.523771, whole genome shotgun sequence.
ACCESSION   DAOOWY010000189 DAOOWY010000000
VERSION     DAOOWY010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41342_length_2516_cov_6.523771"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1982)
                     /locus_tag="PK105_08980"
     CDS             complement(105..1982)
                     /locus_tag="PK105_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61891.1"
                     /translation="MKKFYLIVLQRDRENLPERLRKLGVAHVEELQGIGEKYKNLERE
                     KAEAESVYFILQNYIDKKAKSDKADASISLKYEDCKYVRDIINEISTAKQRLDLLEDR
                     TIDLKREIERVASWGEVSMATLKRVADGAGFSLRFFDAPLKEIAVLPKETEYIRIAAP
                     KGRYRIGLIVREGGKMPDLPPSFIEFLPPEIPLSQMRQEIASMESQKQEIIGEFRNKS
                     VNLSLIKKYLHMLDSNIILERLRSGMPQHESFAYMKGYVPARDCEKFKKIAAKYGWAI
                     AFDDPIEEDQPPTLVENPPAVRIIQPVFEFLGTVPNYWEYDISSWFLIFFSIFFAMIF
                     GDASYGAIIVLATIASIIAGKRKGKPIGDTQKLFLLLGSTTIIWGALTATWFGISFDN
                     LPKLLQDISLPLINGQSPDSENNIKVLCFIIGLVQLSIAHFKNIKRDFPNLKFLSQIG
                     SLLLLEGMFNAGLNLVIDSTRFPIRSWALLCIAIGFFLVFIFGNWNGKLGSSLLESLK
                     TIIPTFLGTISVFADIVSYIRLWAVGLAGLAISQTVNGMASSIMGMALGFPLEFILKL
                     LISILFLIITHLLNFMLTVLSVLVHGIRLNMLEFSGHLGMEWSGYKYKPLKEDEDFAA
                     S"
     gene            complement(1991..>2516)
                     /locus_tag="PK105_08985"
     CDS             complement(1991..>2516)
                     /locus_tag="PK105_08985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOU61892.1"
                     /translation="KKQQLQVEVRSVQTRMEELQAQKTTLEQEAYSWIAVFTEQSAAR
                     TQEDEPLLKVSYINTETGNIAGVAIPVFLGVEFDHKSYDLYATPLWVDAALMLLERLV
                     RTQLELEIVSKQKELLSQELRITTQRVNLFEKVKIPETIADIRKIRIYLGDQETAQVV
                     RGKIAKRKVEGVER"
BASE COUNT          698 a          571 c          502 g          745 t
ORIGIN      
        1 ccgcaattcc tgcgcctatt cctgaccctg ttgctgatag gccaagcaca catgcagcac
       61 caatcaatgc aatattcata gatcactctc ctaaaggtag attgctaaga agctgcaaaa
      121 tcttcatcct cttttagcgg tttatattta tagccggacc attccatacc tagatgtccg
      181 gaaaactcaa gcatattgag tcgtatgcca tgaacgagta ccgaaagcac tgtaagcata
      241 aaattgagca agtgggttat aataagaaac aagattgaaa taagaagttt caggataaat
      301 tctaacggaa atccaagcgc cattcccata atgctggagg ccatgccatt gaccgtttgc
      361 gaaattgcta gtcctgctag tcctacggcc caaagcctaa tataagacac aatgtcagca
      421 aaaacgctga ttgtgccaag aaaagttggt atgatggtct ttaagctttc tagcaaacta
      481 gatccaagct ttccattcca gtttccaaat ataaacacca aaaagaaacc tatcgctata
      541 cataataatg cccagctccg aatggggaag cgcgttgaat ctatgactaa attcagccca
      601 gcattgaaca taccttctag taacaataaa gaacctattt gcgagagaaa ttttaaattc
      661 ggaaaatcac gcttgatgtt cttaaaatga gcgatggata gctgaactag cccaataata
      721 aagcaaagga ccttgatgtt gttttcagaa tccggcgatt gaccatttat aagaggaaga
      781 gaaatatcct gaagtaattt gggaagattg tcgaagctta taccgaacca tgtcgcagtt
      841 agtgcacccc agatgatagt tgtactcccc aagagaagaa ataacttttg tgtatcgccg
      901 atcggctttc cttttctttt tccagcaatg atcgaagcaa ttgtggctag aacgatgata
      961 gctccatagc tagcatctcc aaatatcatt gcaaaaaaaa tcgagaaaaa tattaaaaac
     1021 cagctcgata tgtcatattc ccaataatta ggcacggttc caagaaactc gaatacaggc
     1081 tggattattc gcactgctgg tgggttttcc acaagagttg gaggttggtc ttcttcgatc
     1141 ggatcatcga aggcaatagc ccaaccatat tttgcggcta ttttcttgaa tttttcgcaa
     1201 tcgcgagcag ggacatatcc cttcatatag gcaaaactct cgtgttgcgg catgccggat
     1261 cgcaatcgtt ctaggatgat attggaatca agcatatgta gatatttttt aataagagac
     1321 aagtttactg atttgttccg aaattctcca atgatctcct gcttctggct ttccatcgaa
     1381 gcaatctctt gccgcatctg cgaaagaggg atttccggtg gcaaaaattc tataaaggat
     1441 gggggcaaat ctggcatttt ccccccttcg cggacaatga ggcctatcct gtatctgcct
     1501 ttcggagcgg caatccttat atactcggtc tcttttggca gcacagcgat ctcttttagc
     1561 ggggcatcga agaaacgaag agagaaaccc gcgccatccg cgactcgctt aagtgttgcc
     1621 atcgaaactt ctccccaact cgctactctt tctatttctc tttttaggtc gatagtgcga
     1681 tcttctaata aatccagtct ttgcttagct gtggagattt catttattat atctctgaca
     1741 tatttgcaat cttcatattt cagcgatata gaagcatctg ccttgtcgga cttagccttt
     1801 ttgtcgatat aattctgaag aataaaatag accgattcag cctcagcctt ttctcgctcc
     1861 agatttttgt atttttctcc gataccttga agctcctcta catgggcaac accgagtttt
     1921 ctaagtcgtt cgggtagatt ttctctatct ctctggagca cgatgaggta gaattttttc
     1981 ataggtacaa tcatcgttcg actccttcta cctttctttt tgcgatcttg cccctaacca
     2041 cttgtgcagt ctcttgatcg cccaaataaa tgcgaatctt tcgtatgtcg gcgatggtct
     2101 caggaatttt cactttttcg aagaggttta cgcgctgagt tgttatgcgt agctcctgcg
     2161 aaagtagctc tttttgcttt gagactatct ctagctccaa ttgtgtacga acaagccgct
     2221 ccaaaagcat caatgcagca tcaacccaga gaggagtcgc atacaagtca taactcttat
     2281 ggtcaaattc tacaccaagg aatactggaa tagcgactcc tgcaatgttg ccagtctcag
     2341 tgttaatata gctaaccttt agaagaggtt catcctcttg ggtcctcgca gcgctttgtt
     2401 ctgtgaacac tgctatccac gagtatgctt cttgctcgag cgttgttttc tgtgcttgaa
     2461 gctcctccat gcgagtctgg acgctgcgca cttcgacttg aagctgctgt tttttg
//
LOCUS       DAOOWY010000190         2499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_41732_length_2499_cov_9.831424, whole genome shotgun sequence.
ACCESSION   DAOOWY010000190 DAOOWY010000000
VERSION     DAOOWY010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41732_length_2499_cov_9.831424"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1272
                     /gene="gguB"
                     /locus_tag="PK105_08990"
     CDS             94..1272
                     /gene="gguB"
                     /locus_tag="PK105_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU61893.1"
                     /translation="MSEFMTVVKKNVREYGMYIALAAIMAVFTILSKGIFISSRNIAN
                     LLNQTGYIAVLAVGMTLVIVIRHIDLSVGFLSGFLGAIAAIALTQWNWSLLPTILFVL
                     ALGVVAGLFTALPVASLGIPAFVASLAGWLIYRGALMLVTAGTGTIIIPNEAFNAIGN
                     GYIPDILASETFLPGVHKTTLLLGAIAIVFAIFSQIADRKRKIQYKFEVSSSGLFAAQ
                     LIFVSVILGFITWILAGYNGLSWTIVIMLAVVLVYDFITRRTVLGRHIYAVGGNPEAA
                     ELSGIRVKKITFVVFASMGFLAALSGILFTARLKSATPQAGTLFEMDAIASAYIGGVS
                     AAGGVGSISGSLIGALVYMSLMNGMNLLGTDISLQYIIRGLVLLLAVIFDVMTRKQKA
                     "
     gene            1377..>2499
                     /locus_tag="PK105_08995"
     CDS             1377..>2499
                     /locus_tag="PK105_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61894.1"
                     /translation="MKKFLFGALAVALLMAGCGGGGKSSVIKAGWLGSLTGDQAVWGE
                     CELNTVKMLFEEYNAAGGIEVGGKKYTLEVIGYDNKGDAQESVNATKRLTGQDKVVGI
                     IGPNASGCAIPIAPVLEKAKVPDIATVATNPKVTVQDGKVKPYNFRVCFIDPYQGAVA
                     AGFAYDRLGARTAAVLYDVSDDYSQGLREFFKINFEKKGGTIVADESFNSGDVDFRPQ
                     LSKIKAQNPDVIFMPYFFKEVSLSANQARELGIKATLLGGDGWPSDQLISMGGKAVEG
                     SYFVNHLDYADPAVQDFKNRYREKYGKEAELNGFLAHDAVLLLVEGLKKAGKPNGEAL
                     AKALEGIDVQGITGRIHISPETHNPEGKDAAILKIVNGEY"
BASE COUNT          635 a          541 c          642 g          681 t
ORIGIN      
        1 agcgaatggg acgattactg gcgagctcga tgcaaaatcg gcaacgcaag aagctatcat
       61 gcgccttgcg acacagtact gaggaggaag cagatgagtg agtttatgac tgtggtaaaa
      121 aagaatgttc gcgaatacgg catgtatatc gcccttgcag caattatggc ggtattcacc
      181 attttatcta agggtatatt catatcatcg cgaaatatcg ctaacctcct caatcaaaca
      241 ggatatatag ccgtattggc agtcggcatg actttggtta ttgtaattcg gcatatagac
      301 ctttctgttg gctttctatc agggttttta ggggcaattg ctgccatagc tttgactcaa
      361 tggaattggt cgctattgcc aacaattctg ttcgtgcttg cgttgggtgt ggtagcaggg
      421 ctttttacag cactccctgt ggcgagtctt ggtatccccg ccttcgttgc atctcttgca
      481 gggtggctta tctaccgagg cgcattgatg ttggtcaccg caggtacagg aactatcatc
      541 ataccaaacg aggccttcaa tgctatcggc aacggctata tcccggacat tctggctagt
      601 gagacttttc tgcctggggt tcacaagaca acccttctcc tgggggccat cgccatcgtg
      661 tttgcgattt ttagccagat cgccgatcga aagcgcaaga tccagtacaa attcgaggtc
      721 tcctcttccg gcttattcgc agcccagctc atctttgttt cggtgattct tggattcata
      781 acatggattt tggcaggata taacggctta tcctggacta ttgtgataat gttggcggtt
      841 gtgctggtat acgatttcat cacgcggcgt acggtgcttg gtcgtcatat ttatgccgtt
      901 ggcggaaatc cggaggcggc cgagttatcg ggtatccgcg tcaaaaaaat aacattcgtc
      961 gtatttgcat cgatgggctt cttggctgct ctttccggca ttctattcac cgccaggctc
     1021 aaatcagcga ctccgcaggc aggcactctc ttcgaaatgg atgcaattgc ttctgcatat
     1081 atcggtggcg tatctgcagc cggaggagtt ggttctataa gtggctctct aatcggtgct
     1141 cttgtctata tgtcactcat gaacggcatg aatctgctgg gaacggatat ttctctacag
     1201 tatataatac gtggcttggt tctattattg gccgttatct tcgatgttat gacgcgaaaa
     1261 cagaaagcct agatccagaa ccctgattat attttgcgtt ttcatacttg tgagtacttc
     1321 aatcagcagg tataattatc agaattctat cgcataccca aaggaggtgc tcgagtatga
     1381 agaagtttct atttggagct ctggctgttg ctcttttgat ggcgggctgt ggaggcggag
     1441 gcaaatcgtc cgttataaag gctggttggc tcggttcgct gaccggagat caagccgtat
     1501 ggggcgagtg cgaactcaat accgtaaaaa tgttattcga ggagtataat gcagcgggcg
     1561 gcatcgaagt aggaggcaag aagtacacgc tcgaagtaat aggttatgac aataagggcg
     1621 atgcccaaga atccgttaat gctacaaagc gcctaacagg tcaggacaaa gtagtaggaa
     1681 taattggtcc gaatgcttcg ggctgtgcta ttcctatagc gccagtcctc gaaaaggcaa
     1741 aagtgcccga tatagcgaca gttgctacca atccaaaggt cacggtccag gatggcaagg
     1801 taaagccata taacttccgt gtctgcttta tagatccata tcaaggtgct gtagcagctg
     1861 gttttgccta tgatcgcttg ggagcgcgga ctgctgcggt tctctacgat gtaagcgatg
     1921 attattcaca aggccttcga gagttcttca aaattaactt tgagaaaaag ggtggcacaa
     1981 ttgttgctga cgaatcgttc aactccggcg atgttgactt tagacctcag ctttcaaaga
     2041 ttaaagccca aaacccagat gtcatcttta tgccctattt ctttaaagaa gtatcgcttt
     2101 cggctaatca ggctagagag ctcggcatta aagcaacatt gcttggtggc gacgggtggc
     2161 cttcagacca gctaatctcg atgggcggga aagcggtcga agggagctat tttgtaaatc
     2221 acctcgatta tgctgatcct gcagtccagg atttcaaaaa tcggtataga gagaagtatg
     2281 gaaaggaagc cgagctcaat ggtttcttag cgcatgatgc ggttctgctc ttagtggaag
     2341 ggctaaaaaa agctggcaag ccaaatggtg aagctctagc aaaagccctc gaaggcatcg
     2401 atgttcaagg cattaccggg cgaattcaca taagccctga aacacacaac ccggaaggca
     2461 aggatgctgc aatcctgaag atagtcaatg gagagtata
//
LOCUS       DAOOWY010000191         2475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_42294_length_2475_cov_3.889256, whole genome shotgun sequence.
ACCESSION   DAOOWY010000191 DAOOWY010000000
VERSION     DAOOWY010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42294_length_2475_cov_3.889256"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PK105_09000"
     CDS             <1..924
                     /locus_tag="PK105_09000"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOU61895.1"
                     /translation="FLSVYPHMGLIRDNSKGQIVFNSHFFQMEFSDCETYWDSVGNPF
                     GLLILGGKIQLPPMFDREALIVDANGNISIQTRSIKDQPVMIGTQVYHHGQNCEFYSR
                     PDIARTPSQPGIDLVIVGKRLLGFVAGGDTEIPEGGFIIHISGSSPPDDLMLSYPNKQ
                     GERFAIQVGPAMIIGGEKLRKFHAPMYMSSGVPFPPTVYPSDWDHGRAARMAIGVRSG
                     KPSVVWAAGPDKTSDLWERDSAGASLAEFAEICEDLEFDDAVNLDGGGSAQICLDGMR
                     CFHVSDRDPISGEEAERPIPLALSLPFRLGF"
     gene            1026..2054
                     /locus_tag="PK105_09005"
     CDS             1026..2054
                     /locus_tag="PK105_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU61896.1"
                     /translation="MGNQKISIHEVARLAGVSKSTVSRVISEKGGSVSQETARLVQTA
                     IDQLGYRKNIIAAGFRTQKTYMVLLMIPDVANPFWSEIARSVQDSLEPEGYSVVIGNT
                     DFAEEREARYYELARMSRFDGVILNSVTDNIELIKNLGVPSVLIGERAAAQHIDTVGT
                     DTLQATKIGMEYLYETGHRKIALATSNQGSERYLSLRARAYKDFLYEKSLPIDSSLLF
                     SVNLNEEGGKELVKQLLTSHDWKNRVDSLFCGNDIVAISAMNELRKFGVIAGVDLSII
                     GMDDIPASSQTFPPLTTVRKPRELIGVAAAKLLLRRIEDPHSPIEKHLFPGELVIRNS
                     VIDRRKEK"
     gene            2051..>2475
                     /locus_tag="PK105_09010"
     CDS             2051..>2475
                     /locus_tag="PK105_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU61897.1"
                     /translation="MKSRADILVQPAIFQSRAIGAFIGLAIGDAVGDLGRDQEYRNRY
                     GLISRLFEDGKSTDDTEFGVLTARALLDAHGALTAQKTAEAWRRYILARGGAKKRAGR
                     PLYGAIENLSRGMEPPQSGKFNVMNIDDGAAMRAIPHGIL"
BASE COUNT          649 a          552 c          622 g          652 t
ORIGIN      
        1 ttcctctccg tgtatcctca tatgggatta atccgggata acagcaaggg gcaaattgtc
       61 ttcaactcac atttcttcca gatggagttc agcgactgtg agacatattg ggattctgtt
      121 ggcaatcctt tcggtcttct tattctaggg ggcaaaattc aactccctcc aatgttcgac
      181 cgagaagccc ttattgtaga cgcgaacgga aacatctcga ttcagacacg atctatcaaa
      241 gaccagccgg ttatgatagg cacacaagtg taccaccacg gccagaactg cgaattctac
      301 tctcggcccg atattgcgag gactcctagc caaccgggta tcgaccttgt tatcgtcggg
      361 aaaaggctcc ttggctttgt tgctggcggc gatactgaga tccccgaagg tggttttatc
      421 atccacatct cgggctcttc gcctccagat gacctcatgc tctcttatcc taataagcag
      481 ggtgagcgtt ttgcaatcca ggttgggcca gcgatgatca ttggtgggga aaaattaagg
      541 aaattccatg ccccgatgta tatgagttca ggagttcctt tcccaccgac agtctatccg
      601 tccgactggg atcatgggcg cgctgcgcgc atggcgatag gtgtgcggag tggaaagccc
      661 tctgttgtct gggccgctgg acctgacaaa actagcgatc tctgggagcg cgatagtgcc
      721 ggtgcttcgc ttgctgaatt cgcggaaatc tgcgaggatt tggaattcga tgacgcagtc
      781 aatcttgacg gaggtggctc cgcgcagatt tgtctggatg gaatgcgctg cttccatgtt
      841 tctgatcgtg atccgatttc gggagaggag gctgagcggc ctatcccgct cgcgctttcc
      901 ttgccgttta ggctaggctt ttgaatcgat aacgcttcgc aagtctccgt aaagggaacg
      961 ttcccaaaat aatcattgca tattttgaaa acctgtagta taatgctttt gggaacgttc
     1021 ccactatggg taatcaaaag atcagcattc atgaggttgc acgcctcgca ggagtctcaa
     1081 agagcacagt ttctcgagta atatctgaaa aaggtggctc tgtcagtcaa gaaacagccc
     1141 ggctagtgca aacagctatc gatcagcttg gatatcggaa aaatatcata gcagcaggct
     1201 ttagaacaca aaagacatat atggtacttc taatgattcc agatgtagca aatccattct
     1261 ggtccgaaat agcacgatca gttcaagatt cgcttgagcc agagggctat tcagtggtga
     1321 tcggaaatac tgattttgcc gaggaacgag aagcacgcta ttacgagctt gctcgaatgt
     1381 ctcgatttga tggtgttatc ttgaatagtg ttaccgacaa tattgaactt atcaagaatc
     1441 ttggagtgcc atcagtgctc attggagagc gagctgcagc acagcatatc gatacagtag
     1501 gaactgatac actccaggct acgaaaattg gcatggaata tctctatgaa acaggccata
     1561 gaaaaattgc cctcgcaaca agcaatcagg gcagcgagcg gtatctttcc ttaagagcgc
     1621 gagcatataa agattttctt tatgaaaagt cgcttcctat cgattcctct ttattattct
     1681 ctgtaaattt gaatgaagaa ggcggtaaag agcttgtgaa gcaattgctc acaagccatg
     1741 attggaagaa tagggttgat agcttgttct gtggcaatga tattgtcgca atttctgcaa
     1801 tgaatgagct tagaaaattt ggtgttatcg ctggagtgga tctttctatc attggaatgg
     1861 acgatatccc cgcatcttcc cagacctttc ctccactaac tactgtgcgt aagccgcgtg
     1921 agctgatcgg tgttgctgca gcaaaattac tattaaggcg tattgaagac ccacatagcc
     1981 caatagaaaa acatcttttt cctggtgaac ttgtcatccg gaatagtgtc attgatagaa
     2041 ggaaggaaaa atgaaatctc gagcagacat tcttgtacag ccagcaatat ttcaatcaag
     2101 agctattggc gcttttattg gacttgcgat tggtgatgca gttggtgatc ttgggcgaga
     2161 tcaggaatat cgcaaccgat atggacttat ctctcggctt tttgaagatg gtaaaagcac
     2221 cgatgatacc gaattcggcg tgctcactgc gcgtgcgttg ctcgatgctc acggtgcgct
     2281 cacggcgcag aaaactgcgg aggcgtggcg tcgttacatt ctcgcgcgag gtggtgcaaa
     2341 gaaaagagca ggccggccgc tctacggtgc aatagaaaat ctcagtcgag gcatggaacc
     2401 gccacaatcc ggcaaattca atgtgatgaa tatcgatgat ggtgcagcga tgcgagcaat
     2461 accacatggc attct
//
LOCUS       DAOOWY010000192         2398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_44134_length_2398_cov_24.772941, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000192 DAOOWY010000000
VERSION     DAOOWY010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44134_length_2398_cov_24.772941"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PK105_09015"
     CDS             <1..635
                     /locus_tag="PK105_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61898.1"
                     /translation="MNYSNPELQLLQSIYKATEEGDSYASVSQRQLASSAGLSIGLTN
                     ILLKRFIERGWVKFLHIDGKKIKYALTPSGMEEIAMRAVEYFARAERNTELYRKKIDI
                     FIEGIVRERYKALLLIGLSELDFLFDYACLRYKIGFYKNIKVFMREKDEQDSSWDGLV
                     VVCADNFEDSEEMKGQVGVDIKQYMGIPKEIPLIRFSQILMNIAVKNTSS"
     gene            716..1798
                     /locus_tag="PK105_09020"
     CDS             716..1798
                     /locus_tag="PK105_09020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOU61899.1"
                     /translation="MSYEVAISSAFLSAFTMPIILNFAHKNKLFDSSDARKNHNGNIP
                     RLGGVGMFFAFATMMFALILIQPGTVAKELASRASRLWPIGLAGTLMCIIGLLDDIKS
                     QPAKLKLVIQILAAILVVASGYRFRGFGFHQNAMQGDLAWLSVIISLGWIVGVANAIN
                     FIDGVDGLAGSISFASSLAYGIFYYRVGDQSSSLICLCIAGVIVGFLLFNLPMPRAKI
                     FMGDSGSLFLGFSLAVMPFLGQVPGIDSSKAGLGLIPSITMLGLPVLDALRVMGLRIS
                     QGKNPMSPDRQHLHHLFFDSNHGALRVDITFFLVSAGLALAVLASSVMPRSLGYMIEF
                     IALGLLMLFFRYGRYVNIEHSANAER"
     gene            2178..>2398
                     /locus_tag="PK105_09025"
     CDS             2178..>2398
                     /locus_tag="PK105_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU61900.1"
                     /translation="MKKIICWLLALSIAFIFPAMLTAQERRVALVIGNGDYQAATKLI
                     NPINDAADIGEVLAGLGFDVEVRIDADLDT"
BASE COUNT          673 a          455 c          594 g          676 t
ORIGIN      
        1 agatgaatta ttctaatccg gaactccagc tcctccagtc gatatataag gcgacagaag
       61 aaggggatag ctacgcctcc gtatctcagc ggcagttggc tagcagcgct gggttatcta
      121 ttggcttgac gaatatactc ttgaagcgct tcatagaaag aggctgggtc aaattcctcc
      181 atatcgatgg gaaaaagatc aaatatgcgc ttaccccttc tggaatggaa gagatagcca
      241 tgcgagccgt ggaatacttt gccagggcgg aacgaaatac agagctatat cggaaaaaga
      301 tcgatatctt tatagaagga attgtacgcg agagatataa ggcgttgctc ttaattgggc
      361 tttcggagct cgattttttg ttcgactatg cttgccttag atataagatc ggattctaca
      421 agaatataaa ggtattcatg cgagaaaaag atgagcaaga ttccagttgg gatgggctag
      481 tggtggtctg tgccgataat ttcgaagata gcgaagagat gaaaggccaa gtgggggtag
      541 atataaaaca gtatatgggc atacccaaag aaataccgct catcagattt tcgcagattc
      601 tcatgaatat tgcagtgaaa aatactagtt cataaagcaa accgataaaa gggcttgcaa
      661 agtacacaca aaataatcaa taatagtatt atcaaaaaaa gaaggatcga atgcgatgtc
      721 atacgaagtg gctataagtt ccgcatttct atcggcattc acaatgccta ttatcttgaa
      781 ctttgcgcat aagaataagc ttttcgattc ttctgatgca agaaagaacc ataatggaaa
      841 cattccgcgt ctggggggtg ttggaatgtt ttttgccttt gcaactatga tgttcgcgct
      901 cattttgatt cagcccggga cggttgcaaa agagcttgct tctcgcgcaa gtcggctttg
      961 gccaattggt ttggcgggaa cactcatgtg cataatcggg cttctagatg acattaagtc
     1021 acagccagca aaactcaaac tcgttataca gattctggcg gcaatattgg tagttgcaag
     1081 tggatataga tttagaggtt ttgggtttca tcagaatgcc atgcaagggg atcttgcatg
     1141 gctgtcagtg ataattagcc tgggatggat tgtgggagtt gcaaatgcga taaacttcat
     1201 agatggcgta gacggtctag ccggaagtat atcttttgct tcttcgcttg cctatggaat
     1261 tttctattat cgagttgggg atcaatctag ttcgcttatc tgtctatgca tcgcgggtgt
     1321 aattgtagga tttctattgt tcaatttacc catgcccagg gctaagatat tcatgggaga
     1381 ttctgggagc ctctttcttg gattctctct ggcggttatg cctttcctgg gacaggtgcc
     1441 ggggatagac tcttcaaagg ctggcttggg cttgattcca tcgattacca tgcttggact
     1501 tcctgtctta gatgcgctgc gggtcatggg attacggata tctcagggca agaacccaat
     1561 gtctccagac cgccagcatt tgcatcatct cttttttgat tcaaaccatg gcgcattaag
     1621 agtcgacatc actttcttcc tcgtatcggc tgggctcgct cttgcggtat tggccagctc
     1681 agttatgccc agaagcctgg ggtatatgat cgagtttatc gctttagggc ttcttatgtt
     1741 attttttaga tatggcagat atgtgaatat tgagcactct gcaaatgccg agcgctgagg
     1801 aagccgaaat tttcgggatt cctgtgtctt ggtcttgtat aaagtctgtt tatattgctt
     1861 gtcctaatag accgaatcag gctcgaatag acaagatcgg atttcaatag gcagctcaag
     1921 agaagttatt ttcagtaggt ttacataagg tggagagaag gccgccggaa ttcaagcaaa
     1981 accagaattc cggcggcctt tttttgcttc ggcgaaggta ctatgcaaag gttctatgaa
     2041 aaaggtgcag cggagctaaa tactgcaaaa tataatgcgc aaaggtagaa aaagcattat
     2101 aattcaagat attggctgaa aaagctcaga agaacaagag gattaagaag gccagaaaga
     2161 ttaagaagat taagaatatg aaaaagatta tttgctggct tctcgccctg tctatcgcgt
     2221 ttatctttcc tgcaatgctt acagcgcagg agaggcgcgt cgctctcgta ataggaaatg
     2281 gagattatca agctgctaca aagctcatca atccgataaa cgatgctgcg gatataggag
     2341 aagtgctcgc cggtcttggc ttcgatgtag aagttagaat cgacgccgat ctcgatac
//
LOCUS       DAOOWY010000193         2317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_46239_length_2317_cov_7.835544, whole genome shotgun sequence.
ACCESSION   DAOOWY010000193 DAOOWY010000000
VERSION     DAOOWY010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46239_length_2317_cov_7.835544"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="PK105_09030"
     CDS             <1..1451
                     /locus_tag="PK105_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU61901.1"
                     /translation="AIEDGIIVIDKNGKIEEVNPSMETMLGIKRHDLLGKDFLSIFSL
                     VSSGKEDPEKAEATIKYLLLTPTKVADEDIAFKEPEQQLNVVSSPILDRFGVRKGNVL
                     IFRDITERKKMEEELRKYKESLEELVAARTEQLKKSEEKYKALVDHALVGIGIHQNAQ
                     MVFANKQFASMLRFSEEEIIGLPILQLIHPDERELIMSRAYDRYSGKEVIDTYEMRMV
                     RKDGSFMPAFISNATIEINGARATLITVVDTTETKLRKELEQVNQELERFTYSVSHDL
                     RAPLRSIEGFSRALKEDYEDRLDREGQDYLQRIRAACQRMAAMIDAILQLSRIGRYEM
                     HKEKVNLSAIAQDIASDLETTSPERKVEFSIVEGVFAAGDPMLLRMVLENLIGNAWKF
                     TQEHELGRIEFGVMQQNSKRIYYVRDNGLGFDMKYATKIFDPFQRLDNAKAFSGTGIG
                     LASVKRIIHRHGGEIWAESAVGKGSTFYFTLE"
     gene            1484..>2317
                     /locus_tag="PK105_09035"
     CDS             1484..>2317
                     /locus_tag="PK105_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU61902.1"
                     /translation="MNKSLRVLLIEDSEDDTLLILRELRKGGYFPVHERAETESAMRS
                     ALSSQQWDIILSDYNLPEFCADEALSIFRESGIEAPFIIVSGAIGEEKAVQLMKAGAN
                     DYIMKNNLRRLIPAIERELKESEDRKSRRLAEDSYRKSDFVVNASRDIMALINKSGIC
                     ETVNSSLCKIFGKEEQKEIVGHPVSDVWGNEVYEQKIKPYLERSQNGEEITTEDWFDM
                     EGMGTQCFEINYIPYRDNDGVVTHTIMAVHNITNRKIAEEELDKSYRNLQKTLEETVN
                     AL"
BASE COUNT          742 a          425 c          568 g          582 t
ORIGIN      
        1 aagcaatcga agatgggatc attgttatag ataaaaatgg caaaatagaa gaagttaacc
       61 catccatgga aaccatgctg ggcatcaaga ggcacgatct tcttggtaaa gattttctta
      121 gtatattttc tctagtttcg agcggtaaag aggatcctga aaaagcggaa gctacgataa
      181 aatacttgtt gctcacgcct acaaaggtcg ctgatgaaga catagcattc aaggaacctg
      241 aacagcaact aaacgttgta tcttcgccga ttctggatcg ctttggtgtg agaaaaggta
      301 atgtacttat ttttcgggat ataacagaaa gaaaaaaaat ggaagaggaa ttgagaaagt
      361 ataaggaatc gctcgaagaa ctagttgcag ctcggacaga acagctcaag aaatcggaag
      421 aaaaatacaa agcacttgtg gatcatgctt tggtcggtat tggcattcat cagaacgccc
      481 agatggtctt tgcaaataag cagtttgcat ccatgctgag attctccgaa gaagaaataa
      541 taggtctgcc catcttacaa ctgatacatc cagacgaaag agaacttatt atgtccagag
      601 cctacgatag atatagcggc aaagaagtta tcgacactta tgagatgcga atggtcagaa
      661 aggacggcag ttttatgcct gccttcataa gcaatgcaac gatcgaaatc aatggagcca
      721 gggcgacgct tatcaccgtt gtagatacaa cagaaacgaa gcttcgaaaa gagctggagc
      781 aagtaaacca ggaactcgag agattcacat attctgtctc ccatgatctg cgtgctccgc
      841 ttaggagcat agagggtttc agtcgggctc tgaaagaaga ttatgaagat cgtctagatc
      901 gagaaggaca ggattatctt cagcgcataa gagcggcatg tcaacgaatg gcggctatga
      961 ttgatgctat ccttcagctc tctcgcatcg gaagatatga gatgcataaa gaaaaagtga
     1021 atctcagcgc catagcacag gatatcgctt cagatttgga aacgacatct cccgaacgaa
     1081 aggtggaatt ctcgatagta gaaggtgtct ttgctgctgg agatccaatg cttctgagga
     1141 tggttttgga gaatctgatt ggcaatgcat ggaaattcac acaagaacat gagcttggca
     1201 gaattgaatt tggcgtgatg cagcaaaatt ccaagaggat ctattatgtg cgcgacaatg
     1261 gattgggttt tgacatgaag tatgcgacta agattttcga tccatttcaa cgcctcgata
     1321 atgcgaaagc tttttccggt acaggaattg gccttgcttc ggtaaagcgc attattcacc
     1381 gccatggcgg agagatttgg gctgagagtg ctgtaggcaa aggcagcact ttctatttta
     1441 cattagagta atgacaattt cagatcagca gatggagaat tgtatgaaca agtcattgcg
     1501 agtgcttttg atagaagatt ctgaagatga tacccttttg atcctgcgcg aattgcgtaa
     1561 gggtggctat tttccagtgc atgagcgcgc agagacagaa tccgcaatga gatcagctct
     1621 ctcttctcag caatgggata taatcctctc ggattataac ttgccggaat tctgtgccga
     1681 cgaagcgcta tccattttta gggaaagtgg catagaagcg ccttttataa ttgtttccgg
     1741 agctatcggg gaagaaaaag cggttcaact tatgaaggcg ggtgccaatg attatatcat
     1801 gaaaaataat ctcaggcgcc ttattcccgc aattgagcgc gaacttaagg aatccgagga
     1861 tagaaaatcg cgaagattgg ctgaggattc ttatagaaaa tctgattttg tcgtcaatgc
     1921 ctcgcgcgat attatggcct tgatcaataa atccggaatc tgcgagactg tcaatagcag
     1981 tctttgcaag atttttggga aagaagaaca aaaagaaatt gtcggccatc cagtaagtga
     2041 tgtgtggggc aatgaggtct atgagcaaaa aataaaaccc tacctggaac gcagtcagaa
     2101 tggagaggag attactactg aagattggtt tgatatggaa ggaatgggca ctcaatgttt
     2161 cgaaatcaac tatattcctt atagggacaa cgacggagtt gttacgcata ccataatggc
     2221 agttcacaac atcacaaatc gaaaaatagc ggaagaagag ctagataaga gctatcggaa
     2281 tctgcaaaaa acattagagg agaccgttaa tgcgctt
//
LOCUS       DAOOWY010000194         2313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_46359_length_2313_cov_10.786980, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000194 DAOOWY010000000
VERSION     DAOOWY010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46359_length_2313_cov_10.786980"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..167
                     /locus_tag="PK105_09040"
     CDS             9..167
                     /locus_tag="PK105_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOU61903.1"
                     /translation="MDKQKYYVPADKFDQAKVKAAFRNGILKITVPPKDVVDIPEGIK
                     IEIIKEGE"
     gene            complement(340..729)
                     /locus_tag="PK105_09045"
     CDS             complement(340..729)
                     /locus_tag="PK105_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOU61904.1"
                     /translation="MKELICITCPMGCHLSVDRDELGNMKVTGNRCIRGEQYAREEVL
                     NPKRVVTFTCAALLPENYSSAAESEIVPRVPVRTTNAFPKEMISELLCVLRKQSIRLP
                     VMCGDIVIKDALGTDIDVIVTRTITLP"
     gene            complement(726..1976)
                     /locus_tag="PK105_09050"
     CDS             complement(726..1976)
                     /locus_tag="PK105_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU61905.1"
                     /translation="MKQLIFDAVVIGGGAAGMAAAIELDSRGNSVLVLDREESLGGIL
                     MQCIHNGFGLIEFDEELTGPEFAQRFEEKLQTRNIQVLLKTTVLDIREDDKHKVIYAV
                     SAKKGMMRIEARAIVLAMGCRERNRGNIRIPGARPAGIYTAGLAQRLVNIEGYIPGKD
                     VAIIGSGDIGLIMARRMSWSGCKVHAVIEILPYPSGLTRNIVQCLNDFNIPLYLSHMV
                     TNIYGKDRVEGIEIAPIEAGALMSEKAFRIACDTVLLSVGLVPENELSRDAGIEIHPA
                     TNGPWVDSMLMTTMPGVFACGNVLHVHDLVDYVVEEARRAGANAAAWLSGYRAEREIR
                     VKSGSNIRYIMPMRVNPERENRLYMRALVVKNDARLDVRLDGTEVKSRKLAHVQPSEM
                     LSLTLKPEDLAAARRDSTLEIALV"
     gene            complement(1973..>2313)
                     /locus_tag="PK105_09055"
     CDS             complement(1973..>2313)
                     /locus_tag="PK105_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOU61906.1"
                     /translation="IEKPSWDPYIRKIPRARDADPFALDSLIAQDPAYGNIVCRCECV
                     SEAEIVAAIRAGHTTLDGIKYYTRAQMGRCQGGFCTYKIIRILMRETGMSYDQITKHG
                     GNSVILKGSL"
BASE COUNT          596 a          587 c          529 g          601 t
ORIGIN      
        1 taaaggatat agacaaacaa aagtactatg tccccgctga caaattcgat caagctaagg
       61 taaaggcagc ctttcgaaat ggaatattaa agataacggt tccacccaag gatgtagtcg
      121 atatacctga aggtatcaag atcgaaataa taaaagaagg cgaataacat tggtagcctg
      181 aaaccgtgga caagaaataa cctttgatag caatcgaata gcctttgaga gaagcgcgat
      241 cctcacagac cgcgcttgtt ttgtctactt gttttgtcta ttcgaacaaa ctgctacctc
      301 ttgaaggctg cctcgataaa tccaaagcga gcaactcttt tatggcagcg taattgttct
      361 ggttactatt acatcgatat ctgtgcctag cgcgtcttta atgacaatat ccccacacat
      421 gacgggtaag cgaatgctct gtttccgaag cacgcaaagc aattccgata tcatttcttt
      481 tggaaatgcg ttcgttgttc taacagggac acgaggtaca atctcagatt cggcagctga
      541 agagtaattt tcgggcaata acgctgcaca ggtaaaggtc actactcgct tgggattcag
      601 gacttcctct cgggcatact gttccccgcg tatgcagcga ttgccagtaa ccttcatatt
      661 accgagctca tctctatcga cagagagatg gcagcccatt ggacaggtaa tacaaatcag
      721 ttctttcata caagtgcgat ctctagggtt gaatctcgtc ttgctgctgc aagatcttct
      781 ggctttagtg tgaggctgag catctcagat ggttgcacgt gggcgagctt ccgcgatttt
      841 acttctgtac catcgagacg cacatcgagc cgcgcatcat ttttgacaac gagagcgcgc
      901 atataaagcc tattctcacg ctctgggttg acacgcattg gcatgatata gcgaatattc
      961 gaaccgctct ttacgcgaat ttcacgctct gccctataac ccgaaagcca agcagcagca
     1021 ttagcgcctg ctcttcgcgc ttcttccact acataatcga caagatcatg aacgtgcaaa
     1081 acattgccgc aggcaaatac gcctggcata gtcgtcatga gcatactatc gacccaaggt
     1141 ccattagtag ccggatggat ctcgattcct gcatcccgtg agagctcgtt ttccggtaca
     1201 aggccgacgg ataagagcac ggtatcgcat gcaatccgaa aggccttctc gctcataagc
     1261 gctccagctt cgattggagc aatctcgata ccctcaaccc ggtccttgcc atatatattg
     1321 gtcaccatat gagaaagata tagtggtata ttgaaatcat ttagacattg aacaatgttt
     1381 cgtgtgagcc cacttggata aggaagaatt tcgatcaccg catgcacctt gcagcccgac
     1441 cagctcatac ggcgagccat tataaggcca atgtcgccag aaccgatgat tgctacatct
     1501 ttgcccggaa tatagccttc gatattgacg aggcgctggg caagacctgc ggtgtagata
     1561 cccgctggcc gtgccccggg aatgcgaatg ttccctcgat tccgctccct gcagcccata
     1621 gccagaacga ttgcccgcgc ttcgatgcgc atcatgcctt tctttgccga aacagcatag
     1681 atcaccttgt gcttgtcatc ttcacggata tcgagcactg tggtcttgag caagacctga
     1741 atatttctcg tttgtaattt ttcctcgaat cgctgggcaa attcagggcc tgtaagctct
     1801 tcgtcgaatt cgattagtcc aaatccatta tgaatgcatt gcatcaggat tccaccaaga
     1861 ctttcctccc gatctagaac gagtacggaa ttcccccggg aatctagttc gattgcagcg
     1921 gccattccag cagctcctcc gcctattaca accgcatcga agataagttg cttcataggc
     1981 tccccttgag aatgacagaa ttgccaccat gcttggtaat ctggtcgtag ctcattcctg
     2041 tctcgcgcat gagtattcgg attatcttat atgtacagaa acctccctga catcgcccca
     2101 tttgagcgcg agtatagtat ttaatgccat ctagggtagt atgccccgct cggattgccg
     2161 cgactatctc ggcttcgctt acacattcgc agcgacagac aatgttgccg tatgcggggt
     2221 cctgcgcaat gagagaatct aatgcgaaag gatcggcatc gcgggcgcgg gggattttgc
     2281 gaatgtatgg gtcccaggag ggtttttcga tga
//
LOCUS       DAOOWY010000195         2309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_46470_length_2309_cov_14.543035, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000195 DAOOWY010000000
VERSION     DAOOWY010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46470_length_2309_cov_14.543035"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..867
                     /locus_tag="PK105_09060"
     CDS             190..867
                     /locus_tag="PK105_09060"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU61907.1"
                     /translation="MRLTRFFDIVLSFIAIVILLPFMIPIMIILKLTGEHHIFYRQPR
                     VGKGGKDFYVFKFATMLKDSPNMPGGVLTQKNDPRILPFGRFLRKTKINELPQLVNIL
                     IGQMSFVGPRPQARKHYELYPAEVREAIDKFRPGLTGLASMIFRNESEILDKVGGDRD
                     HFHDTVIAPYKGELELWYSHHCGWRSYFLLIALTIWVLFRPDDSKAWRHFYHDIPKPP
                     KELEPYL"
     gene            951..>2309
                     /locus_tag="PK105_09065"
     CDS             951..>2309
                     /locus_tag="PK105_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOU61908.1"
                     /translation="MTKNKGSFFIAIFILGFAALCNIAAQSTDQAALLQQMQFGGPAG
                     TGSGLTGAAPTVAPQQPTVNQLSTTAAQPTPSATAQALGTQAAAAQTAQLEALPLSTI
                     EKMFALMASNLGTPAQNLTQFGYSLFEAPSAPSLASIGDDYILGPGDSLVLYLWGDPV
                     DIKELSSSYLLTVDRNGAIFLPPVGQVSVWGQDLGSVKSVLKAMLDRRYKKLEMNLTL
                     STLRQFPVFVSGYAASPGTVLANGADTVLSVLSRAGGILKTGSLRSVMLTRQNKDGGV
                     QKIEIDFYQSLLEGKPLDLRVREGDSIFIPGIGPVVALAGELKRPGIYELKGAPTMAQ
                     VLSYAGGMLPSSRSSSVTLLRFDAEGKILQTADVTDKAFAEFAAGDGDFVYFGRVSDL
                     LLGQAQISGAVKYPGRYEVRSFKTLKELLEKAQLLPETNLFYGRVYRTDQSGRDKSFA
                     FSP"
BASE COUNT          591 a          576 c          576 g          566 t
ORIGIN      
        1 atctttttaa tgccactatc ccagagtttg cacataaaga gaccggcaaa ttcctcgatg
       61 agagaatgtg aatcgaaggg tatcaaagga atcaatcttt atgtataaat gaggcaacaa
      121 aatcaaagat aagtacatga aaaaattgtt gtaaaggggg cttcttcaaa aatgactatt
      181 gaggcgcaaa tgcgtctgac acgatttttt gatatcgtgc tcagcttcat cgccattgtg
      241 atcttactcc ccttcatgat tcctattatg atcattctca agctgacagg ggagcatcac
      301 atcttctatc gtcaacctag agtcggcaaa ggtgggaaag atttctatgt gttcaaattc
      361 gcgaccatgc tgaaggatag ccccaatatg ccaggaggag tgctcaccca gaaaaacgat
      421 ccccgaattc ttcctttcgg tcgctttctt cgaaaaacga aaatcaacga attaccacag
      481 cttgtcaata tcctcatagg tcagatgagt tttgtaggtc ctcggcctca agcgagaaag
      541 cattatgagc tctatccagc tgaagtgcgg gaagcaatcg acaaatttcg gccaggactg
      601 acaggactcg cctcgatgat ctttcgcaat gaatccgaga tactggacaa agtgggcgga
      661 gacagagatc atttccacga taccgtgatc gccccctaca aaggcgaact tgagctttgg
      721 tatagccatc attgcggctg gcgttcttac ttcctgctta tcgcgctcac tatctgggtg
      781 ctctttcgcc cagacgactc aaaagcctgg agacatttct atcacgatat tccgaaacct
      841 ccaaaggaat tggagcctta tctctaagaa taaagtagct ttctactcat ttgttgagca
      901 gcagattatt aatcttgaaa aaatagagta aaattcatac ggagcgaata atgacaaaga
      961 acaaaggatc cttttttata gcaattttta tccttggatt cgccgcgctc tgcaatatcg
     1021 ctgctcaatc cacagatcaa gcagcgcttc tgcagcaaat gcagtttgga ggcccagcag
     1081 gaactggaag tggtcttacg ggcgctgccc cgacagtggc gcctcaacag cccacggtca
     1141 atcaactgag cacaacagcc gctcagccaa ctcctagcgc tacagcgcag gctctaggaa
     1201 cgcaggctgc tgcagcccaa acagcgcagt tagaggctct gccgctctcg accatcgaga
     1261 agatgtttgc actgatggcc tctaatcttg gcacaccagc acagaacctt acacagtttg
     1321 gctattcgct ctttgaggca ccaagcgcgc cttcgctcgc ttcgattggc gacgactata
     1381 tccttggtcc tggcgatagc cttgtgctct atctctgggg agatccagtc gatatcaagg
     1441 aactctcgtc gagctacctg ctcactgtgg acagaaatgg tgcaattttc ctgccccccg
     1501 tgggacaggt gtcggtgtgg ggtcaggacc tcggttcggt caagagcgtg ctcaaagcaa
     1561 tgctcgatcg acgttacaaa aagcttgaga tgaatctcac gctctctacg ctgcggcaat
     1621 tccctgtctt tgtctcaggg tatgcggcaa gtccaggtac agtgcttgcc aatggagctg
     1681 atacagtgct cagtgtgctc tcaagggcag gtggcatttt gaagacgggc tctctgcgct
     1741 cggtgatgct gacgcgtcag aataaggatg gaggcgttca gaaaatcgag atcgatttct
     1801 accagagtct ccttgaggga aagccgctcg acctacgagt gcgcgagggc gattcaattt
     1861 tcatcccagg gatcgggcct gtggttgcac ttgcgggcga gcttaaaagg ccgggcatct
     1921 acgagctcaa aggggcgccc accatggcac aggtgctctc atatgcggga ggtatgcttc
     1981 cttcgtctcg ctcaagcagt gtgacactcc tgcgctttga tgcagagggc aagattctac
     2041 aaaccgcgga cgtgacagat aaggcatttg cagagtttgc agcaggcgat ggggactttg
     2101 tctattttgg gagagtaagc gatcttctct tggggcaggc tcagatctcg ggcgcagtga
     2161 agtatccggg tcgctatgaa gtgcgctcgt ttaagacact gaaggagctt ctcgaaaagg
     2221 cgcaactctt accagaaacc aatctattct atggccgcgt ctatcgcacg gatcaatcag
     2281 gccgcgacaa gtcgttcgca ttctcgcca
//
LOCUS       DAOOWY010000196         2306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_46568_length_2306_cov_32.828521, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000196 DAOOWY010000000
VERSION     DAOOWY010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46568_length_2306_cov_32.828521"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK105_09070"
     CDS             complement(<1..210)
                     /locus_tag="PK105_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61909.1"
                     /translation="MPTKEEEILNAFASAENMETDPFLMDLEELFNSLESKGSSKEKG
                     IEKARSIGERALSAAKDLVDSLTTQI"
     gene            complement(493..2040)
                     /locus_tag="PK105_09075"
     CDS             complement(493..2040)
                     /locus_tag="PK105_09075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU61910.1"
                     /translation="MKSGDTVGTYLLEQRLGKGVTASTWLAKPAQSEENGLDEDNSAS
                     AALSAQACVLKIFDIAETSSWKPLDQFRREADILRTLNHPRIPKYIESFETTTEEKFL
                     FVLVMEYIEGDTVEAYIASGKKFTEKQIEELLAELADILAYLGSLRPPIVHRDINPRN
                     LIIRPDGHIVLVDFSGVQDAIRTALYPGATLVGTAGYIPMEQVSGRATHRSDLYGAAA
                     TTVFMLTGRNPSELPMRNLKIDLSGLLYMSPRLSFVLDNWLDPDPDRRTLSAIKAAAV
                     LREKIVLPPETRTSIDARTEATSAENNQNGAGPSWSGAFPRDSFLGEILHNLEERTEQ
                     RVYPSELPSNSQLIIQDLAPGLYIKMPKRSNSSMRGGTIFPIAWMGFVFFWTTMTLRM
                     HAPFFFSLFSIPFWIAGFSMIKSFLGPSMTETELFLTSDGLLRKSSGFLSNSAQQCRL
                     SDIREAKVHKSSVRINKRYLKELAIKLKGGTISFGLGLSDPELYYLEKRFNEELILLK
                     QLPAGES"
BASE COUNT          611 a          515 c          504 g          676 t
ORIGIN      
        1 aatttgcgtt gttaggctat caactagatc ctttgctgca gaaagagccc tttctccaat
       61 gcttctggct ttttctattc ccttctcttt gcttgaaccc ttgctctcta atgaattaaa
      121 taattcttca agatccatta aaaatggatc tgtctccata ttctcagcgc ttgcaaaagc
      181 atttaatatt tcttcttctt ttgttggcac attagtagcg ccactctttt ttaggctgct
      241 ggcagaatat tctttgactt cgggagcttg agatgagaaa attgagcaac cggcaaggaa
      301 aaccaaaaag atcgccaata tggcgaatac ctttttcatt ttagcctcct ctcatattct
      361 tctaagataa tagactatat tcaattctag cgcaaaaaaa acataagtca agaaaaatta
      421 gctacctttg cacaaaattg tcgtgtaccc tatatgggct agcttcgata atgaataaga
      481 gccaatacag acttaacttt ctcctgccgg cagctgcttg aggaggataa gctcttcgtt
      541 gaaacgcttt tcaaggtaat atagctcagg gtcggacagc ccaaggccaa aactgatggt
      601 gccacccttt agctttattg caagttcttt gaggtatcgc ttatttatcc gcacagagct
      661 cttatgtacc tttgcttccc gaatatcaga gagtcggcat tgctgcgcgg aattcgatag
      721 aaatccagag ctttttcgaa ggagcccatc cgatgtcaaa aagagttcag tctcagtcat
      781 agatggccct aggaaggatt ttatcattga aaaaccggca atccaaaatg gtatgctgaa
      841 aagagaaaag aaaaaaggcg catgcatgcg caaggtcatc gtggtccaga aaaaaacaaa
      901 acccatccat gcgataggaa agatagtgcc ccctcgcatg ctgctattgc ttctttttgg
      961 catttttata tagagcccag gtgcaagatc ctgaattata agctggctat tcgaaggtaa
     1021 ttccgaaggg tagactctct gttcggtcct ctcttccaga ttgtgcagga tctctcctaa
     1081 aaaggaatcc cgaggaaagg ctccgctcca agaaggtcca gctccatttt ggttgttttc
     1141 ggccgaggtt gcttcagttc ttgcatctat gcttgtcctg gtctccgggg ggaggacaat
     1201 cttttctctc aaaactgctg ccgccttgat tgcagaaaga gttcgacggt ctggatccgg
     1261 gtcgagccag ttatccaaca caaaagagag tctcggcgac atatacagaa gtcccgatag
     1321 atctattttg agattccgca ttggcaattc tgaaggattt cgccctgtga gcatgaaaac
     1381 agttgttgcg gcggcaccat agaggtcgga acgatgggtg gctcgacccg atacctgctc
     1441 cattggtata taaccggcgg tgcctaccag tgtggcacca ggataaaggg ctgtgcgaat
     1501 ggcatcctga accccggaaa agtcgacgag tactatgtgt ccatccggcc taattattag
     1561 atttcgagga ttgatgtctc tatgcacaat tggaggacgc agactgccca gataagccag
     1621 tatatccgca agctccgcaa gcaactcttc gatttgcttt tcggtgaatt tcttgcccga
     1681 ggctatatag gcttcgaccg tatctccttc tatgtattcc ataacaagca cgaaaaggaa
     1741 tttctcttct gtggtcgttt cgaaactctc tatgtatttt gggattctcg gatgatttag
     1801 ggtgcggaga atgtcggctt cgcgtctaaa ttgatccagg ggcttccagg aactggtctc
     1861 ggctatatcg aatatcttga gcacacaagc ttgggcagaa agggcagcgg aagcagaatt
     1921 gtcctcgtcg agcccatttt cttcggactg tgccggtttg gcaagccaag tagatgccgt
     1981 tacacctttg ccgagccttt gctcgagcaa atatgtgcct acagtatccc cagatttcac
     2041 tctttttcct tatagccttt caaatcgata ggcaaaataa cttgcttctt gaggtaataa
     2101 atgctcacgt aattccgagt attcggcatt gccttgttct ccataagaaa gataattcaa
     2161 atacagactt tcgtcatcta tcctagggtc ggatttagcc caaaaaggac ttgagttttt
     2221 tcggtttagg ctgtatgata tattcgttac tgatttatcg ataattctat atttagattt
     2281 cctaatatta tttaagagga gctaca
//
LOCUS       DAOOWY010000197         2263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_47783_length_2263_cov_6.592844, whole genome shotgun sequence.
ACCESSION   DAOOWY010000197 DAOOWY010000000
VERSION     DAOOWY010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47783_length_2263_cov_6.592844"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..2089)
                     /locus_tag="PK105_09080"
     CDS             complement(296..2089)
                     /locus_tag="PK105_09080"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOU61911.1"
                     /translation="MKAKWNGNPKSLMRTVVIVSILVASILMPLGALEQTAYQEGFAI
                     GSSQASEIQQNVATMKSTAQLRGTDFSRVLALAQENETLLATILPEKLEWMHGVADGS
                     RIPYDDILIYNTADRLMTGFVGECTTFIAQGNVLASGEGSLIAKNRDLGPNVISEVSK
                     EIGHEYGPNEIYKAAYIDIPQAPRTYSFVGSRSAGRWGYGMGINEFQVIVSDNDAPTR
                     VELAFTKGLHDNDYVRLVLERARTAREGVEILTKLTEKYGQAWNSIMFEIGDPQELWI
                     VEITNTQWVARRYTNCYTVRTNQFQITDDYELCSDDLITYAQAQGWLSKDVSKTQKIN
                     FRAVYGTIELYPSDNENFASRPAVETLYNTQVRYDRGMELLSKISGNISPKTIMPCTR
                     DHYDTYTLPSGKVLQLDQVPYYSTDYVYERQEWVAEYPTKDTVEVSIYPRSICHHAFE
                     GSTASTAILVARPDVENELGLMLHAYCQPCDSLFVPFYVGVSSVDSRYSTPEVGSKFL
                     QISKISFGCYKTYHEGIRKIFDPYEATLFEEMPEMEKQYMNLKVSGKSMDAQMLLDTF
                     SMQHWQEAIELTDNALESMTKLTVESSAWSR"
BASE COUNT          594 a          500 c          491 g          678 t
ORIGIN      
        1 cctactataa tgacggtagc catgttcatt tcagcattca tgacgaacaa tatggcgagt
       61 caccagaaat gccaagcccg cttacctact tcgagccttc ggtggcagaa gaactagagg
      121 ctatgaatgt tttatggtga attgagtgtc ttccttaggg aagtaatgta atatcggtga
      181 gtgcctgcaa ggcagaattg ctattgcgca ttttttcgcg tttttctcgc acaaagggtt
      241 ataaggatat ccactcaaga aaggctgcct atgccatgag gcagcccttc tttgcttatc
      301 tgctccaggc tgaagattct acggtgagtt ttgtcattga ttctaatgcg ttatctgtaa
      361 gttcaattgc ctcttgccaa tgctgcatgg agaaagtatc aagcagcatt tgagcatcca
      421 tcgattttcc cgatacttta agattcatat actgcttctc catttcgggc atttcttcaa
      481 aaagggttgc ttcataaggg tcaaatattt tccttatacc ctcatgatac gtcttatagc
      541 atccaaagga tatttttgag atttgaagga actttgaacc tacctcaggg gtagaatacc
      601 ttgaatctac cgaggatacc cctacataga aaggcacaaa aaggctatca catggctgac
      661 aatatgcatg gagcatgaga ccaagctcat tctcaacatc aggtcgggca accaagattg
      721 cggtagaagc ggtactgcct tcaaatgcat gatggcagat tgatcgtggg tatattgaca
      781 cctcgactgt atctttggtg gggtattcag ccacccactc ctgcctttca tagacatagt
      841 cagtactgta atagggcacc tggtcaagct gtaatacctt cccgcttgga agtgtatagg
      901 tatcataatg gtctctggta catggcataa tggtttttgg tgatatgttc cctgaaattt
      961 tactgagaag ttccatacct cggtcatacc gcacttgtgt attatagaga gtctctacag
     1021 ctggacgtga ggcaaaattt tcattatcag aaggataaag ctcgattgtg ccatagactg
     1081 ctctaaaatt gatcttttgg gtctttgaga catccttact caaccatcct tgtgcctgcg
     1141 cataagtgat aaggtcatcg gagcaaagtt cataatcatc ggtgatctgg aattgatttg
     1201 tccttacagt gtagcaattt gtataccgcc gcgcgaccca ttgcgtgttt gtgatctcga
     1261 cgatccaaag ctcttgggga tcaccaattt cgaacatgat agaattccag gcttgcccat
     1321 atttttcagt gagctttgtt agtatttcaa caccttcgcg tgcagtcctc gccctttcca
     1381 aaacgagccg cacataatcg ttatcatgaa ggcctttggt aaaggccaat tcgactcttg
     1441 tgggagcatc attatcagaa actataacct gaaattcatt gattcccatg ccatatcccc
     1501 agcggccggc tgaacgggaa ccaacaaaag agtaggtgcg tggagcctga ggaatatcga
     1561 tatatgcagc cttgtagatt tcattcgggc catattcatg ccctatttct ttcgagacct
     1621 ctgagatgac attaggacct agatcgcgat ttttcgcaat aaggctccct tctccagatg
     1681 ccagtacatt gccttgggct atgaaggtag tgcattcccc gacaaatccc gtcataagcc
     1741 tatctgcggt gttgtagatg aggatgtcat cataaggtat acgtgaacca tctgcgacac
     1801 catgcatcca ttcgagcttc tctggcaaga ttgtggcgag gagcgtctca ttttcctgtg
     1861 cgagcgccaa tactctcgaa aagtcggtac ctctcagctg ggcagttgac ttcattgttg
     1921 caacattttg ttggatttcg ctcgcttgag aagagcctat cgcaaagcct tcttgatatg
     1981 ctgtttgctc aagcgcaccg agtggcatga gtatcgatgc taccaatatc gacacaatga
     2041 cgacggtacg catcaatgac tttggattgc cattccattt tgctttcata attgcctcct
     2101 agaaaaatat tagcctagaa attttctcaa tgcaagtttg ggcgatatgg tagcttggct
     2161 tagcaaaaga ttagtctcaa taggcaaaat atagcgcagt gtctatatgc tatattgctt
     2221 tattaaagac atttctttta cagatttctt gttctataat caa
//
LOCUS       DAOOWY010000198         2236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_48526_length_2236_cov_23.493810, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000198 DAOOWY010000000
VERSION     DAOOWY010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48526_length_2236_cov_23.493810"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK105_09085"
     CDS             complement(<1..268)
                     /locus_tag="PK105_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61912.1"
                     /translation="MIRKIISGVLGVVIALAVGALIMLLQGYEPLATYGALAQFSIVG
                     LGPFTTTLKNAVPLVLTGLSAAIAFASGPVNLGQPGQFAFGALAA"
     gene            complement(265..1929)
                     /locus_tag="PK105_09090"
     CDS             complement(265..1929)
                     /locus_tag="PK105_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61913.1"
                     /translation="MRTQLPAPSLIKKMLNAKSKPNIKDRPSLLSLSLISTILNRKWQ
                     LMDDFISLERIVKVYPPNVLAVNNVSVGIRKGEIHAIIGENGAGKSTLMKILYGLIPF
                     DSGTVRLAGNPVHFKKPADAIAMGIGMVHQEIELISQYAVWENVVLGAEPIQGAIGKL
                     NVKQAIELVRAKIDEFQFNLDPEAIVDRISVAARQKVEILKLLYRNVSVLILDEPTSV
                     LTPQEIPQLFAELKRLRDKGTTILFISHRLDEVLELSDRITVMRKGQKIATVVAAETN
                     KAELAQMMVGRQVIFTSKRTTHKAGECVFETKNLNLVRRDGFKLLDDISFSVHAGEIV
                     GIAGVEGNGQLELVNCVMGLAEPSSGHIEVSNKEITQLPILERRKLISFVPQDRAKMG
                     ASISASILENVIMTHHRLNPDFSKWRGLVLNYQTARAFVESLVERFSVQMASYQNPFR
                     SLSGGNQQKAILGRELLLPTPFFLMDQPTRGLDVGSIEYVHDQILSMRKENRAILVIS
                     ADLEELFLIADRILVMHRGRIVADLVTDQTSIEEVGRYMLEGKLTA"
BASE COUNT          543 a          578 c          546 g          569 t
ORIGIN      
        1 tcgccgcaag agctccgaat gcgaactgcc cgggctgtcc taggttgaca ggcccagagg
       61 caaaagcgat tgccgcggac aatccggtca gtacaagcgg cacggcgttc ttgagggtag
      121 tagtaaatgg cccaagcccg acaatagaga attgcgcaag agctccgtat gttgccaaag
      181 gctcataacc ctgcagaagc atgattagag cacctaccgc tagcgcgatt acaacaccaa
      241 ggacaccact gattattttg cgtatcatgc cgtaagcttc ccttctagca tatagcgccc
      301 tacttcttcg atcgaggtct gatcggtaac gagatccgcc acgatccttc cgcgatgcat
      361 cacgagtatt cgatccgcaa tcaaaaaaag ttcttcgaga tccgccgaga tgacaagaat
      421 ggctcgattt tccttgcgca tagagagaat ctgatcatgc acatactcga tcgacccgac
      481 atctagccct cgcgttggct gatccatcaa gaagaaagga gtgggcagca ataattcgcg
      541 gccaaggatc gccttttgct gatttccccc cgatagcgat cgaaatgggt tctgatatga
      601 tgccatctgc acagaaaatc gctctacaag agattccaca aaagcccgcg ccgtttggta
      661 gttgagcaca agacccctcc atttcgaaaa atccggattg agccgatggt gcgtcataat
      721 gacattttcc aatatcgacg cagaaatgct cgcgcccatc ttggctcgat cttgaggcac
      781 aaatgaaatc aatttgcgcc gttctaggat ggggagttgg gtgatctcct tgttgctgac
      841 ttctatatgc ccgctgcttg gctcggctaa tcccatgaca cagttgacca gttccaactg
      901 accattgcct tccacacctg caatgccgac aatttcaccc gcatgaaccg aaaacgaaat
      961 atcatcaaga agcttaaaac catcgcgtcg cacaaggttc agattcttcg tttcgaacac
     1021 gcactcgccc gccttatggg tcgtacgctt tgaggtaaaa atgacctggc ggccaaccat
     1081 catttgggcg agctctgcct tgtttgtttc cgcagcgacc accgtggcga ttttttggcc
     1141 tttgcgcata acggttatgc gatcgcttag ttcgagcacc tcgtcgaggc gatgggaaat
     1201 gaagagaatc gtcgtgccct tgtcgcgcaa gcgctttagc tctgcaaaaa gctgcggaat
     1261 ttcctgagga gtaagcaccg atgttggttc gtcgagtatg agaaccgaga catttcggta
     1321 gagcagtttg aggatttcga ccttctggcg agcggctaca gagatgcggt caacaatggc
     1381 ctctggatcg aggttgaact gaaattcgtc gatttttgct cgcacgagct cgatggcctg
     1441 tttgacattg agcttaccta tcgcgccctg aatgggctcc gcgccgagca cgacattttc
     1501 ccagaccgca tactgagaga tgagctcgat ttcctgatga accatcccga ttcccattgc
     1561 tatcgcatcg gcgggcttct tgaagtgaac gggattgcca gcgagtcgga cagtaccaga
     1621 atcgaagggt attagtccat aaaggatctt catcaaggtg cttttgcctg cgccattctc
     1681 tcctattatc gcatgtatct cgccttttcg aattccgacg gagacattgt tcaccgcgag
     1741 gacattgggc ggatatactt tgacaatacg ttcgagcgaa ataaaatcat ccatgagttg
     1801 ccactttcga ttcagaatcg ttgaaatcaa tgatagagat aataagcttg gtctgtcttt
     1861 tatattgggc ttgctcttgg cattgagcat tttcttgatt aaagaaggcg ctggaagctg
     1921 agtgcgcata gcgcttcgcc agcgggcgcc actgttgatt ttgcgattca acgctagcgg
     1981 cgcccatggt aatcttatgg cttttccctg gcagagctca tctttttcgg acaagcgcca
     2041 atattattct gacaggtcat ggaacagctt cgaatcctag acgatcatca ctgccagacg
     2101 ttctcgccat tgtatagagg aaccttcagc tttccatcta tgatctgctg gcgcgccgta
     2161 ttgactttgt cgacgatttt cttgggaaga acctgctgtt ttgcatcgaa gacgaggtct
     2221 acaccaccgc ttgcaa
//
LOCUS       DAOOWY010000199         2213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_49204_length_2213_cov_60.629750, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000199 DAOOWY010000000
VERSION     DAOOWY010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49204_length_2213_cov_60.629750"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="PK105_09095"
     CDS             <1..1601
                     /locus_tag="PK105_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU61914.1"
                     /translation="IVVYSTSAGKVAQDGTGRNGTFTEELLRHLPTPGLDIMEVLRRT
                     GQDVQAKTASAQIPAIYSQFFGFLQLVPGEAPAMAGPAAPMETSAKASPAAGSVTSAG
                     SSSGSSSESSGVLDYLPAFFDEAPANSKLTIAKAEELTWDGQWLSAWRILEKTDPNSR
                     DPYMLAEKIRTMLNGNTSLDLFQGFSIANMPPDGDWEAACEAGTLDEDYFDFDPHSAV
                     QALLKRGVKVPPVLAAALGDFYYSAYYDYDDSSFLSLDEIRDNALHWYDIANENFVLV
                     ELESIMHYAELLMDAGRADEAVSILEDETEWEPEDIELRLQLIDAYIATGDDSSAFSE
                     LDWLIGKAASNEDAREFYRRAFELALKIQDQQALNRYLAAFEKKYPNDWLAGLVRHRV
                     AVQTGNYMAAQTIADGLMKQFPLNTELLEGLLSNWLDNLNYNSTIAEAGLAFLDRWVV
                     ASQWNPVALGTYCLYRSLYRYLIIQNWPPSYMKNMAIQNALTDLNVAEMNLSVARNTL
                     SEEGKALLDMIPDFRNELQAEMGY"
     gene            complement(1725..>2213)
                     /locus_tag="PK105_09100"
     CDS             complement(1725..>2213)
                     /locus_tag="PK105_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU61915.1"
                     /translation="AMAYGYALHAAICGKDIEGILSEAKKGAEAAQAAPDSQVYPQCG
                     ASVVARLLHFKELASSFSSPDEVLDFIYYVYGTTLASVDVATAAMCIFLSAKDDVWLS
                     IRMGTSVGGDTDTIAALAGALSAAYALSHGQSLNIPKAIVSEVLENNKLDFDRLAERL
                     YL"
BASE COUNT          578 a          511 c          579 g          545 t
ORIGIN      
        1 gcatcgttgt gtattcgaca agcgctggga aggttgccca ggatggaact ggtcgaaatg
       61 gcacatttac agaagagctt ttacggcatt tgccaacccc tgggctcgat ataatggaag
      121 tattgcggcg cacaggccag gatgtgcagg caaaaaccgc cagcgcgcag attccggcga
      181 tctacagcca attctttgga tttttgcagt tggtgccagg agaagctccg gcaatggcag
      241 gtcctgcggc tccaatggaa acaagcgcca aggcgagtcc tgctgcaggc tccgtaacca
      301 gcgctggttc tagttctgga tcgagctctg agtcaagtgg agtgctggat tatctaccgg
      361 ctttttttga tgaagcgcct gcgaatagca agcttaccat tgctaaggct gaagagctaa
      421 catgggatgg tcaatggctc tcggcctgga ggattctcga aaaaactgac ccaaatagtc
      481 gcgaccctta catgcttgct gaaaaaatac gcacaatgct caatggcaac acatcgctcg
      541 atttgtttca gggtttttcg atagccaata tgccacccga tggagattgg gaggctgctt
      601 gtgaggccgg aactctagat gaggattatt tcgatttcga tccccatagt gcagtacaag
      661 ctctcttaaa gcgaggtgtc aaagttccgc cagtgctggc ggcagcgctc ggcgatttct
      721 attacagtgc ctactatgat tatgacgata gttcttttct gagcctcgat gagataaggg
      781 acaatgctct ccactggtat gatatcgcaa acgaaaactt cgttcttgtc gagttggaga
      841 gtattatgca ctatgccgag cttcttatgg acgcaggccg ggcagatgaa gctgtttcaa
      901 ttcttgaaga tgaaacagaa tgggagcccg aagacatcga attgaggcta cagctcatag
      961 atgcatatat cgcgacgggg gacgattcga gcgccttttc tgagctcgat tggctcatag
     1021 ggaaggcagc ttcgaatgag gatgcacgcg aattctaccg cagagcattc gaattggctc
     1081 tcaaaataca agaccagcag gcactgaatc gatacctagc agcattcgag aagaaatatc
     1141 ccaatgactg gctcgctggg cttgtacgtc acagagtagc tgtccagaca ggcaattata
     1201 tggctgctca aactattgcc gacggactca tgaagcaatt tcctctcaat actgagcttc
     1261 ttgagggtct tttgagcaac tggctagata atttgaacta caattctacg attgccgagg
     1321 cggggcttgc ctttcttgac cgttgggttg tggcgtctca gtggaatcct gtagcgctgg
     1381 ggacttattg tctatatagg tctctatatc gctatctaat tattcagaac tggccgcctt
     1441 cttatatgaa aaacatggcc atacagaatg cgcttaccga tctcaatgta gctgagatga
     1501 acctttcggt cgcaagaaat acgctttccg aagaaggtaa ggctctttta gatatgatac
     1561 cagatttccg aaatgaactt caggctgaga tggggtacta aagttgctag gcggtcgcgt
     1621 tcatctagat tttaagtctg gataagcaaa agcacgctac aaatagtggt gcgctacaag
     1681 tagaggtgct ctatgagcag aggcgcacta caagtagaag tgctttataa gtaaagacgc
     1741 tctgcaagac gatcaaaatc gagcttgttg ttttcgagca cttcgctgac aattgccttt
     1801 ggtatattga gagactgacc atgagataag gcgtaagcag cgctgagcgc tccggcaaga
     1861 gcggctattg tatcggtatc gccgcccaca gaggttccca ttcggatgga gagccagaca
     1921 tcgtccttag cagacaggaa aatgcacata gcagccgtag ctacgtccac tgatgctagt
     1981 gtggttccat acacatagta gataaagtct aaaacctcgt cggggctgga aaaagaagag
     2041 gccaattcct tgaaatgtaa aagccgcgcc acaacagaag cgccacactg aggatagact
     2101 tgagagtctg gcgctgcctg agccgcttcc gcgccctttt tggcctccga caagatccct
     2161 tcgatatcct ttccgcagat agccgcatgg agcgcgtagc cataggccat cgc
//
LOCUS       DAOOWY010000200         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_49773_length_2195_cov_9.692056, whole genome shotgun sequence.
ACCESSION   DAOOWY010000200 DAOOWY010000000
VERSION     DAOOWY010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49773_length_2195_cov_9.692056"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /gene="gguA"
                     /locus_tag="PK105_09105"
     CDS             complement(<1..1462)
                     /gene="gguA"
                     /locus_tag="PK105_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU61916.1"
                     /translation="MNDNILEMRHITKTFPGVRALNDVSFAVRRGEIHCLVGENGAGK
                     STLMKVLSGLYPHNEFEGEIILNGKKVEFNGIHDSEEAGVGIIYQELALVPEMTVYEN
                     LMLGHEIRRGMTIDVHEMIRKAEDLLKRVRLSINPTAKIKDLGIGKKQLVVIAKALSR
                     NVSLLILDEPTATLNEDDCENLLDIIRMLKMQGVTSIMISHKLKEVLSIAETITVLRD
                     GQTICTLDRRMDDVNEQVLIRHMVGRSIENIYPPRISKPEETISIEVKNWNAYDPKLG
                     RKVLNNVNFYVRKGEVVGLAGLVGSGRTELARSLFGNPDKYEISGDLILKGEKRRFSH
                     PSQAIKAGLAYATEDRKANGLILIKSIQENISLANLQEVSKNGLINEELDKQTALSFV
                     ESLSIKTPGIHQLVMNLSGGNQQKVSVAKWLFVGPDVLIFDEPTRGIDVGAKYEIYNI
                     INDLVAKGMSILMISSELPEILGMSDRIYVVANGTIT"
     gene            complement(1564..>2195)
                     /locus_tag="PK105_09110"
     CDS             complement(1564..>2195)
                     /locus_tag="PK105_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU61917.1"
                     /translation="LYAGAASDNNSFIFFEGAWSALQPKIADGTYIVRNSDKAAALAK
                     KAKLTRDEAAQIIGQVTTNWNFSDAKNKAEANLTAAPKEAKGTVYICAPNDGTARAIA
                     DAFAADKDVKTYYITGQDAEIASIQYIIDGKQSMTVLKDVRTLVKDAISAATAYMKGQ
                     TPPVTAYYNNGKKDVPAKPTAIVTVTKENVKKEIIDSGYWPADKFTGLK"
BASE COUNT          507 a          542 c          486 g          660 t
ORIGIN      
        1 cagtaatcgt cccattcgct acgacataga tacgatcgct catgcccagt atttcgggca
       61 attcggacga tatcatcaaa atactcatac cctttgccac cagatcgttg ataatgttat
      121 atatttcgta ttttgcacca acatcgatac ccctggttgg ctcatcgaat atgagcacat
      181 cgggtccaac aaatagccat tttgcaacgg atactttctg ctgatttccg ccagagagat
      241 tcataacaag ctggtgaatt cccggcgttt ttatcgagag ggattccaca aagcttaagg
      301 cggtctgctt atcgagttct tcgttgatca ggccattttt cgatacctct tgtaaattag
      361 caagagaaat attttcctgg atcgatttga taagtataag gccgttggct ttccggtctt
      421 ccgtagcata ggccaatccc gccttaatgg cttggctggg atgagagaag cgccgcttct
      481 ctccctttag aatgaggtca cctgaaatct catatttatc aggattgccg aagagacttc
      541 tcgccaattc ggttcttccg gagcctacaa ggccagctag cccgacaacc tcccctttgc
      601 gaacatagaa attgacattg ttcagcactt ttcgtccaag cttcggatcg taagcattcc
      661 aattctttac ttcaattgaa attgtctctt caggtttgga tatgcgagga ggatagatgt
      721 tctctatgga acgcccaacc atgtgcctaa tgagaacttg ctcgttgacg tcatccatgc
      781 ggcgatccag ggtgcatatg gtctgaccat cgcggagaac tgttatcgtt tcagcaatgg
      841 agagcacctc ctttaactta tgagaaatca tgatggaagt gacgccttgc atcttcagca
      901 ttcttattat gtctagcaaa ttttcacagt catcttcgtt caatgtggct gtcggttcat
      961 cgaggataag aagcgataca tttcggctta gtgcttttgc aatcacgaca agctgttttt
     1021 tgccgatacc taggtcctta atcttagcgg tcgggttgat ggaaagtcta accctcttga
     1081 gaagatcttc tgctttcctt atcatttcgt gcacatcgat ggtcatacca cgtctgattt
     1141 catgccctag catgaggttc tcatagacgg tcatctcggg caccagagct agctcttgat
     1201 agataatgcc tacgcctgcc tcctcgctat cgtggatacc attgaactct actttctttc
     1261 cattgaggat tatctcgcct tcgaattcat tgtgcggata taaaccgctc agaaccttca
     1321 taagtgtgga ttttccagcc ccattttccc ctaccaaaca gtggatctct ccgcggcgaa
     1381 ctgcaaagct gacatcattc aatgctctaa cacctggaaa ggtctttgtt atatgccgca
     1441 tttcgagaat attgtcattc ataatctgta ccgcctggat tgatcttact acaaacgaga
     1501 gccgctcgta atgagcggct ctcctgatcg tactcaaatg ggcatgctca cataccccat
     1561 tgatcacttg agaccagtga acttgtctgc aggccaatag ccagaatcga tgatctcttt
     1621 ctttacgttc tctttggtca ccgtaacaat ggcagtcggc ttagccggta catctttttt
     1681 gccattgttg taataggcag taaccggcgg ggtctgtccc ttcatgtatg cggtagctgc
     1741 actgatcgca tcctttacta gcgtgcgcac atccttgagg acagtcatgg attgtttgcc
     1801 gtcgatgatg tactgaatgg aagctatctc ggcatcctgg ccagtgatgt aataggtctt
     1861 gacatcctta tctgcggcaa aggcatccgc tattgcacgg gcggtgccat cgttaggagc
     1921 gcagatatac acggtccctt tggcttcttt aggagccgct gtcagatttg cttcggcctt
     1981 gttcttggca tcgctgaagt tccagttggt cgtgacttga ccaatgatct gagctgcttc
     2041 gtcgcgagtg agcttggctt tctttgcaag agccgcagcc ttgtccgaat tcctcacgat
     2101 atatgtgcca tcggctattt tgggctggag ggcgctccat gctccttcaa agaatatgaa
     2161 agagttattg tcacttgctg ctcctgcata cagat
//
LOCUS       DAOOWY010000201         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_51551_length_2141_cov_22.019655, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000201 DAOOWY010000000
VERSION     DAOOWY010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51551_length_2141_cov_22.019655"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PK105_09115"
     CDS             <1..383
                     /locus_tag="PK105_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61918.1"
                     /translation="DSLDEDLDPKTSWALRHLDQFPVELATSDYEQILRVPGIGPATA
                     RRILNLRRAGRLNYSSLRKLRMHLQKAFWFITINGKILPEEMQNCRSINIAYALEHSE
                     ILHELLKEKTSPPAPIQPELDFSP"
     gene            459..1361
                     /locus_tag="PK105_09120"
     CDS             459..1361
                     /locus_tag="PK105_09120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU61919.1"
                     /translation="MKKSVGFWKRTIIGVIIILIVLPTIGAILLAKDNIRLQKEILSI
                     SPNGDDVITVGTNSYDAASIVRKRPLLSYQLLYPELCANFMGFNDDLVDSKTVFLTFD
                     DGPSSSTPAILDVLKNHGIKASFFVNGKSSIFATSWLRRTAQEGHTIGMHSYTHKYTL
                     IYDSVENFIEDLNKNYLYIKNNTGISPTILRFPGGSINTYNLEIYQSLCAEVLRRGFI
                     YCDWNVSSGDALKEATTDSIVDNVINGVSLCWGPSFVLMHDNGNNELVQALPKIIDKL
                     KAKGYSFAAFDNTVRPPMFVYPEE"
     gene            1373..1975
                     /locus_tag="PK105_09125"
     CDS             1373..1975
                     /locus_tag="PK105_09125"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU61920.1"
                     /translation="MKEKNNLTKAFDELLHGKSYELENEQPSIDAAHDPDPQPSAAQL
                     ASLAASTASPARQSSEATITADMVIRGTICSASNINVFGTVFGDVSCENDMIVSGKIE
                     GNISARSLQLLSGAIIGDIEARSIVEIVQGSTVNGNISAERIAVDSRVIGNLNAAALV
                     RLNANAVIDGNIKSGSLAVQEGCQLKGNVDIHKAEEGEST"
     gene            complement(2003..2137)
                     /locus_tag="PK105_09130"
     CDS             complement(2003..2137)
                     /locus_tag="PK105_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61921.1"
                     /translation="MAPKGVKVFNPAFDVTDSKLITAIITEKGVLKPPYRDSLRKIME
                     "
BASE COUNT          630 a          477 c          453 g          581 t
ORIGIN      
        1 aagactcctt agatgaagat ttagatccga agacttcgtg ggctctgcga catctagacc
       61 aattcccagt agagcttgca acttcagact atgagcagat cctgcgagtc ccaggaattg
      121 gccccgctac tgcgcgcaga atactaaatc ttcgtagggc tggaagattg aactactcct
      181 ctctgcgtaa acttcgcatg catttgcaaa aggcgttttg gttcataacg ataaacggaa
      241 aaatcttgcc cgaggagatg cagaattgtc gaagcataaa tatagcttat gctcttgaac
      301 attccgaaat tctacatgaa cttctcaaag aaaaaaccag ccctccagcg ccgattcaac
      361 ctgaattaga cttttcaccc taatgtataa atctgattga tgtaaatctg attgacttaa
      421 accctaccct gaagcaaaat aagacaaggt cttataaaat gaaaaaaagc gtcgggttct
      481 ggaagcgcac cataatagga gtaataatta ttctcatcgt tttgcccacg attggagcga
      541 tactccttgc aaaagataat atccgtctgc aaaaagagat cctatctata tctcccaatg
      601 gagatgatgt gatcaccgta ggcacaaata gctatgatgc ggcttcaatc gtaagaaaaa
      661 gaccgcttct gtcttatcaa ctcctatatc ccgagctttg cgcaaatttt atgggattca
      721 atgacgacct tgtcgattca aagactgttt tcttgacttt tgatgatgga ccctcttctt
      781 caactcctgc gattctcgat gtgctaaaga accatggcat aaaagcttca ttcttcgtaa
      841 acggaaaatc gagcatattt gctacctcat ggcttcgacg aacagcacaa gaaggccata
      901 caattgggat gcatagctat actcacaaat atacgcttat ctatgattca gtggaaaatt
      961 tcatagaaga tctcaataaa aattatcttt atatcaaaaa caacactggc ataagcccaa
     1021 cgattcttcg gttcccagga ggaagcatca atacttataa tttggaaatc taccaatcgc
     1081 tctgtgcgga agtgcttcga cgaggcttca tatattgtga ttggaatgtg agttcaggcg
     1141 atgcgctgaa agaagcgaca acagattcca ttgtcgataa tgttatcaat ggagtcagcc
     1201 tctgctgggg accttccttc gttctgatgc acgacaatgg aaacaatgag cttgtacagg
     1261 ctttgcctaa gattatagac aaactgaaag ccaagggata ttcctttgcc gcatttgata
     1321 atacggtgcg gccaccaatg ttcgtctatc ctgaagaata aggaggaaaa caatgaaaga
     1381 gaagaataac ttgacaaaag cctttgacga gcttttgcat ggcaaatctt acgagctaga
     1441 aaacgaacag ccttcgatcg atgcggctca tgatcctgac ccacaaccct ccgctgcaca
     1501 acttgcgagc ctcgctgctt cgaccgcttc gcctgcccga caatcctctg aggctacaat
     1561 tactgcggat atggtgatcc gaggtactat ttgcagtgct tccaatatca atgtatttgg
     1621 aaccgtgttc ggcgatgtct cctgcgagaa cgatatgata gtctccggca aaatcgaagg
     1681 aaacatctct gcccgctccc ttcagcttct ttcaggtgca atcataggag atatagaagc
     1741 tcgatctatc gtcgaaatag tccagggctc taccgtcaac gggaatattt ccgccgaacg
     1801 gattgccgtg gatagtcgag tgattgggaa tctaaatgct gcagcactcg ttcggctcaa
     1861 tgcaaatgct gtaatcgatg gaaatatcaa atcgggttct cttgctgttc aggaaggatg
     1921 tcaattgaaa gggaatgtcg atattcataa ggcggaggag ggagagtcta cctgataggc
     1981 tgatttcttt ccttaataat tcttattcca taatttttcg aagcgagtcc ctatagggcg
     2041 gctttagcac acctttttcc gtgattattg cggttataag cttcgaatcc gttacatcga
     2101 atgcaggatt gaatacttta acaccctttg gagccattgg t
//
LOCUS       DAOOWY010000202         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_51554_length_2141_cov_9.054171, whole genome shotgun sequence.
ACCESSION   DAOOWY010000202 DAOOWY010000000
VERSION     DAOOWY010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51554_length_2141_cov_9.054171"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PK105_09135"
     CDS             <1..589
                     /locus_tag="PK105_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61922.1"
                     /translation="PRAGSVNLDSRPLISYSARERSQLIAFLPQLEKLAFNYRVMDFV
                     LMGRTPRMEVLALPGLEDEEAALQALSLCKLDSFSMRNIGELSGGEFQLVRIARCLAQ
                     GASILILDEPASMLDPAHSRHIADVLASLADSGITIIYTTHDIELGLFLGGEALILSG
                     GMIQWSGPAEGLHNTEILTRTYGISFSNKEIPSAF"
     gene            848..1276
                     /locus_tag="PK105_09140"
     CDS             848..1276
                     /locus_tag="PK105_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61923.1"
                     /translation="MKRSIAISIIVCLVVLSAKAQVITFGLGDAELEAGLNELNISAK
                     VNMQVFQTEVSMVWGQPTASITFALSQGLTPGEVYLAAALAHLSSKPLNIVVELYQKN
                     KQKGWGALAKDLGIKPGSKEFKQLKEQLAGSSKKLKSQKK"
     gene            complement(1447..>2141)
                     /locus_tag="PK105_09145"
     CDS             complement(1447..>2141)
                     /locus_tag="PK105_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOU61924.1"
                     /translation="HLVHYFGGLDKLKGKKITMSWAYSPSYGKPLSVPQGIIGLASRF
                     GMDIVLAYPKGYDLIPDVVELAKKQAIDSGGSFTYVDSMDEAFKDANIVYPKSWAPYD
                     VMLRRVPLLHAGDKEGLKELEREALANNAKFKDWECTEEKMKLTRSAAPNSSQALYMH
                     CLPADITGVSCAAGEVAASVFDRYRDATYREASFKPYIIAAMILLGKFERPAELLESL
                     LTRGRQRTYGNF"
BASE COUNT          520 a          502 c          536 g          583 t
ORIGIN      
        1 tccgcgcgcc ggctccgtca acctcgatag tcgcccgctt atttcctatt cggctagaga
       61 gcgctcgcaa ttgatagcat ttttgccaca actcgaaaaa ctcgctttca attatcgagt
      121 aatggatttt gtattgatgg gtcggacgcc tcgcatggaa gttctggcct tgcctggatt
      181 ggaggatgaa gaagccgcat tgcaggcgct ttctctttgc aagctcgatt cctttagcat
      241 gcgcaatatt ggtgagcttt ccggtggcga gtttcagctt gtgcggatcg cgcgttgcct
      301 tgctcagggg gcttctattc taattctgga tgagccagca agcatgctgg atccagcgca
      361 ttcgcggcat atcgctgatg tgctcgcatc gctcgccgat tcaggtataa caataatcta
      421 tacaacgcac gatatcgaat tgggcctttt tcttggaggg gaagcgctga tactttcggg
      481 aggcatgatt cagtggagtg ggcctgctga gggcttacac aatactgaga ttctcacccg
      541 gacttacggt atctcttttt caaacaaaga gataccgagt gcattttgag gcatatggag
      601 cagctatgct tgatattatg atgaatcgaa tctataaacc tgtgcgcacg caagggcagg
      661 atcactctgc tttttgcgtc tcgagatacc gaattcaatc aggcagtcgt gctccatcat
      721 tatctcatcg aagaggctca agcccggtct aaactatgac atactaatgg gcgcaactat
      781 cggcatttat tatagttcat attgttggtg cgcttttgcc atagtatatt tgaaaaaggg
      841 gtgttctatg aagcgatcga ttgcaatttc cattatcgta tgtttagtag tactgagcgc
      901 taaagcgcaa gtcatcactt ttggtctcgg tgatgctgag cttgaagctg gcttgaatga
      961 gctcaatata agtgcaaaag tcaatatgca ggtctttcag acagaagtct ccatggtctg
     1021 gggacagccc acagcgagca tcactttcgc gttatcgcaa gggcttacgc ctggcgaagt
     1081 atatcttgct gcagcactgg cccatctttc tagtaagccg cttaacattg tggttgagct
     1141 gtatcaaaag aataaacaaa aaggctgggg cgctcttgca aaggatcttg gcataaagcc
     1201 aggttccaag gaattcaaac agctgaagga acaacttgca ggctctagca agaagctgaa
     1261 atcgcaaaaa aagtgatggg actttatata gggtagtgaa aactcaaaaa gcggcgccat
     1321 tctaggcagc ctccttcgct ttctttgcat tttagggtca cttgtagtat cgctctctgt
     1381 atcactgtga gaaaagcaag tagactctag ataagctcgg tgctctagta ttcttagacc
     1441 ctagaatcag aagttgccat aagtcctctg tctgcctcta gtcaagaggc tctcgagtag
     1501 ctcagccggc cgctcaaatt tacctaaaag aatcatcgcg gcgatgatat agggcttaaa
     1561 gctcgcttcg cgataggtcg catcgcggta gcgatcgaac acgctagcgg cgacttcgcc
     1621 ggcagcgcag gagacacccg ttatatctgc gggcaaacaa tgcatataga gcgcctgtga
     1681 ggaatttggt gccgcgctcc ttgtgagctt catcttttct tcggtgcatt cccagtcctt
     1741 gaattttgca ttgttggcga gagcttctct ttcgagttct ttcaagcctt ctttatctcc
     1801 agcatggaga aggggcacgc ggcggagcat aacatcgtat ggcgcccagc ttttgggata
     1861 cacaatattg gcatctttga atgcttcgtc catcgaatcg acatatgtaa aactgccgcc
     1921 cgagtctatc gcctgctttt tagccagttc gacgacatca gggattagat cgtatccctt
     1981 tggatatgca agcacaatgt ccatgccgaa gcggcttgca agaccaatga tgccctgtgg
     2041 cacggacagc ggcttgccat aagaggggct gtaagcccag ctcatcgtga tctttttgcc
     2101 tttgagcttg tcgaggcctc caaagtaatg cacaaggtgc a
//
LOCUS       DAOOWY010000203         2109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_52581_length_2109_cov_9.224927, whole genome shotgun sequence.
ACCESSION   DAOOWY010000203 DAOOWY010000000
VERSION     DAOOWY010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52581_length_2109_cov_9.224927"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2109
                     /locus_tag="PK105_09150"
     CDS             <1..>2109
                     /locus_tag="PK105_09150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU61925.1"
                     /translation="YEGEEVKLYWANYDQYYIKTSEYLRDYAFRLRPENTVNPMRVHF
                     KLVDAAEGEHDNVKEVQGKERKFKLALDRPVSIESGELVIHFTYEPAQEKQKDLNAAT
                     EAAILALDDPAVVTWIELLGRKYRRADGTESDNTWLRIHLDRYTARNTFDYFIHKDLK
                     AFLSRELDFYIKNEVMHLDDIESESAPKVEQYLSKIKVIRTIAGKIIDFLAQLEDFQK
                     KLWLKKKFVTETSWCVRVGIVPKEFYPEIAANEAQWEEWKALHGLEGEVFASKGGDLF
                     TSQAVPGTVEFLSAYPTLMIDTCHFSAGFTARLLDALDDIDEKTDGVLVHSENFQALN
                     LMQRRYREKIKCVYIDPPYNTDSGDFLYKDDYQHSTWLSMMNDRLCIVAPILRPDTLA
                     FIHIDEREQTRLDMLIQQLFGADNLLGPFVWIARVGKAVTERVLQVKHEYVMCARVSD
                     KGRLELVKTDVANCRYKDERGGYDREQLRQWGGQHDRREDRPTLYFPIPTPFGIDVFP
                     RRPDGSDGCWRASREQVAKMLEAGDLDFVQDSKTAEITVYRKIREGTVTISAPSNILD
                     NCGTSTTATKEIKGLFFDKTFNTAKPIGLAKRIFQLIPDNLNAAILDYFAGSGTTGHA
                     VINLNREDGGKRKFILVEMADYFDTVLLPRLKKVTFTPEWKDGKPKRLATTEEAERSP
                     RVFKVIRLESYEDTLNNLEL"
BASE COUNT          589 a          459 c          549 g          512 t
ORIGIN      
        1 cgtacgaagg cgaagaggtc aagctctatt gggccaatta cgatcaatac tatattaaaa
       61 cgagcgagta tttgcgggac tatgcgttca ggttgcgtcc agagaatacg gttaatccga
      121 tgcgcgtgca ttttaagctc gtcgatgccg cggagggcga gcacgataat gtaaaagaag
      181 tgcagggcaa agaacgaaag ttcaaactgg ctctggacag gcctgtatcg atcgagtccg
      241 gcgagcttgt catccatttt acctacgaac cagctcaaga aaagcaaaag gacttgaacg
      301 ccgccaccga agcggcgatt ctggcattgg atgatcccgc agttgttaca tggatagagc
      361 tgctcgggcg caagtatcgc cgcgcggatg gtacggagtc cgacaatacc tggttgcgca
      421 tccatctcga ccgctacacc gcgcgcaaca cctttgacta tttcattcac aaggacctca
      481 aggctttcct tagccgcgag ctcgatttct atatcaagaa cgaagtgatg cacctggacg
      541 atatcgagag cgagagtgcg cccaaagtag agcagtatct ttccaagatc aaggtaatcc
      601 gcaccatcgc aggaaaaatt atcgattttc ttgcgcagct tgaggacttt cagaagaagc
      661 tgtggctcaa aaagaaattc gttaccgaaa cgagctggtg tgtgcgcgtc ggtattgtcc
      721 ccaaagaatt ctatcctgaa attgctgcga acgaggcgca gtgggaagaa tggaaggctc
      781 tgcatggtct ggaaggagag gtctttgcgt cgaaaggtgg cgatttattc acatcgcaag
      841 ctgtgcctgg cacggttgaa ttcttgtcgg cctatccaac tttgatgatc gacacgtgcc
      901 atttcagcgc cggttttacc gcacgccttc tcgatgcgtt ggacgacatc gatgagaaaa
      961 ctgatggcgt cctcgtccac agcgagaact ttcaagcgct gaacctgatg cagcgcaggt
     1021 atcgcgagaa gataaagtgc gtatatatcg atccaccgta caatacagat agtggtgatt
     1081 ttctttataa agatgattat cagcattcta catggttaag tatgatgaat gacagattgt
     1141 gtatagtggc tcctattttg agacctgata cccttgcttt cattcatata gacgaaagag
     1201 agcagacaag gctcgatatg ctaattcaac agttgttcgg ggcagataat ttattgggtc
     1261 cttttgtatg gattgcaaga gtaggaaagg cagtaacaga aagagtactt caggtcaaac
     1321 atgagtacgt aatgtgcgct cgtgtttcag ataagggaag gttggaactt gtaaaaactg
     1381 atgttgcaaa ctgtcgctat aaagacgaaa ggggcggata tgaccgtgag caattgcggc
     1441 aatggggtgg ccagcatgat cgaagagagg accgtccaac gctctatttc cctatcccaa
     1501 ctccctttgg tattgatgtt tttccaagga ggccagatgg atcagatggc tgttggcgtg
     1561 ctagtcgtga gcaggtggca aaaatgcttg aagcgggtga tttggatttt gtccaagaca
     1621 gtaaaacagc cgagataaca gtttatcgta aaatacgtga aggtacagtt accatatcgg
     1681 ccccttcaaa catactagat aattgtggga catcaactac tgcgacaaag gaaattaagg
     1741 gtttattttt cgataaaacg tttaatacag caaaaccaat agggttagct aaacgaattt
     1801 ttcagttaat tccagacaat ctgaatgcag cgatattaga ctactttgct ggttccggca
     1861 ctaccggcca cgcggtcatc aatctcaacc gtgaagatgg gggcaaacgc aagttcatcc
     1921 tggtcgagat ggcggactat ttcgatacgg tgctgctgcc tcgacttaag aaggtaacct
     1981 ttaccccgga gtggaaagat ggcaagccaa agcgtctcgc caccacagaa gaagctgagc
     2041 gcagcccgcg cgtgttcaag gtgatccgtc tcgaatcgta tgaggacacg ctcaacaact
     2101 tggaattgc
//
LOCUS       DAOOWY010000204         2096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_53024_length_2096_cov_11.494855, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000204 DAOOWY010000000
VERSION     DAOOWY010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53024_length_2096_cov_11.494855"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PK105_09155"
     CDS             <1..488
                     /locus_tag="PK105_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61926.1"
                     /translation="KESIREGSLWDLLEVRCRAHPRMLDGLRRLAEKKEWLERLDSAR
                     KSTFFCTSAEGSLRPEVVRYARRIDRISLQGNVLITDDSHMEASGYDRVLYWKPPFGP
                     YPAELAETYPFNAEVPEQADSSAFEQADKILQELIRNNPEARYCLRLKREGLEKTLPE
                     I"
     gene            complement(608..1591)
                     /locus_tag="PK105_09160"
     CDS             complement(608..1591)
                     /locus_tag="PK105_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61927.1"
                     /translation="MEMRKMFGIFLLLAIYIYTGAMACAIEYPQFKSSVSTTGITVNF
                     DTLYQKADKINMVHDIKDHDSKHIIWAYKDTGNWAIVKDDGALVIATPSAFSLDVKGA
                     LNRDLYRAKNLITSIGGRISEEPSVYYVDDPYLYRFITYDFGNDFYLKLSIPNCTLQK
                     AMLSVSGSDYTEIATSDNELKVPGQHYLIDGIEVSGCDGTYCSVGMGAGGIKPRGISC
                     VPQSCESNSMTGLEHTSVSVEPVDITNKISPGIHTISATDIGSQHTMTMEAVTSSSKD
                     EMLLFPDDYSWVINETRSKPMAELYALIMPSQASNSSTSELTSLANSTDSS"
     gene            complement(1792..2019)
                     /locus_tag="PK105_09165"
     CDS             complement(1792..2019)
                     /locus_tag="PK105_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61928.1"
                     /translation="MDFRAESSLSSHPPLLASLVAENVIKGGMIPKVEACVRVVRDGV
                     EKAHIIDGRKHHAILLELLTDQGVGTMIRRN"
BASE COUNT          569 a          499 c          476 g          552 t
ORIGIN      
        1 tgaaagagag cattcgcgag ggctcattat gggatttatt ggaggtgcgc tgccgggcgc
       61 atccgcgcat gctggatggc ctgcgaaggc tggccgaaaa gaaagaatgg ctggagaggc
      121 tggactcggc tcgcaagtcc accttcttct gcaccagcgc cgagggctca ttgcgacccg
      181 aggtggtgcg ctacgcccgg cgcatcgacc gaatatcttt gcaggggaat gttctgatca
      241 ccgatgactc acatatggag gccagcggat acgatcgagt gctttattgg aagccaccct
      301 tcggccccta tcctgccgag ctggccgaga cctatccctt caatgccgag gtgccggagc
      361 aggccgatag ctcggctttc gagcaggcgg acaagatcct gcaagagctg atccggaaca
      421 atccagaggc caggtattgc ctaaggctga agcgcgaagg attggaaaaa acactgccag
      481 agatttagat cccatttttt tttgccaaag gtatctgaaa agcccacata gcggcgaaag
      541 aatcaataag agtgaggcaa aaaagaaaga tcaaggtagc tatctgtttc tgctacctga
      601 aattaaccta gctggaatca gtcgaatttg ccaaacttgt tagctcagat gtactggaat
      661 ttgaagcctg gctgggcata attagagcat agagctcagc cataggcttg gagcgggttt
      721 catttattac ccagctgtaa tcatctggga ataacagcat ttcatccttt gaggatgatg
      781 tcacagcctc catagtcatt gtatgctggc taccaatatc ggttgctgag atcgtatgaa
      841 taccgggcga aattttattt gttatatcaa caggttccac acttacacta gtatgttcca
      901 aaccagtcat tgaattgctc tcgcatgatt gaggaacaca ggaaatgcca cgtggtttaa
      961 tgccaccggc acccatgcca acgctgcaat aagttccatc acaaccggag acttctatac
     1021 catctattaa ataatgctgt ccaggtactt tgagctcgtt atctgatgtg gcaatttccg
     1081 tataatctga accagataca cttaacatcg ctttctgcag agtgcaatta gggatgctca
     1141 attttaagta gaaatcattt ccaaagtcat aggttatgaa gcgatacaga tatggatcat
     1201 cgacataata aaccgatggc tcttccgata tacgaccacc tatagatgta atcaagtttt
     1261 ttgctctgta taaatcgcga tttaatgccc ccttgacatc taaactaaaa gccgatggag
     1321 ttgcaatgac caaggcacca tcatctttta caatagccca gttgccagtg tctttataag
     1381 cccaaataat atgcttgcta tcatggtctt ttatatcgtg gaccatattt attttatctg
     1441 ctttttgata gagagtatcg aaatttacag ttattcctgt tgtcgatact gacgatttga
     1501 attgcgggta ctcaattgcg caagccatag cgcctgtata gatatatatt gctagcaata
     1561 aaaatattcc aaacattttc ctcatttcca taacctccta aactaagaac ccacctatta
     1621 tcataaatac ttagctagac tcccaaaaaa ttgcagaaaa ctgatgagca tataatttaa
     1681 aataggttaa ttgatgcctg aaatgcttta ccggatcact ttgtctcgaa ggtgaccatg
     1741 caatccgcta ggcaactggc atcggtgagc aaacatcttt ttctgcgagc tttaattccg
     1801 tcggatcata gtgcccacgc cctggtctgt gagcagctcc agaagtatgg cgtgatgctt
     1861 tctgccatct atgatatggg ccttttccac accgtcgcgc acgacgcgaa cgcaggcttc
     1921 caccttggga atcattccgc ccttgatgac attctccgcc accaagctcg cgagcaatgg
     1981 cgggtggctg gagaggctgg actccgcacg aaagtccaca ttcttttata caagctccga
     2041 aggctccctg cggcctgagg tggtacgcta cgcccggcgc atagaccgaa tatctt
//
LOCUS       DAOOWY010000205         2093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_53127_length_2093_cov_14.795388, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000205 DAOOWY010000000
VERSION     DAOOWY010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53127_length_2093_cov_14.795388"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..404
                     /locus_tag="PK105_09170"
     CDS             183..404
                     /locus_tag="PK105_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61929.1"
                     /translation="MAMRSFSLYSDAPNDIKELLLNGKRIDDFLPPPYQLVKRVPKVK
                     VTIALNQQSLEFFKKAEVTASQTLDPGEG"
     gene            761..>2093
                     /locus_tag="PK105_09175"
     CDS             761..>2093
                     /locus_tag="PK105_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU61930.1"
                     /translation="MPRGTQIINGVEYVFETKAKWNPDKKYGTHSRRYIGKMVDGVFV
                     PNKRYELEEELKEAKKPGHVPVTISERLFCGATMLLDSIGEKLGIREDLEKCFPQDYK
                     KILSLAYFLLLEDHSPLSRFPRWTMLHRHPYGKIISSPRSSELFAALDEDSQQRFFHL
                     QMQRRMEHEYLAYDITSISSYSELIRFVKYGKNKEHDALPQINLALLYGESSRLPVYY
                     RSLPGNISDVTTVKRLIDELAYLSIEKVKLVLDRGFYSEENVNGLLSHHYKFLISLKT
                     SLRLVQTMLDEVRESLPTRQYYNSNFRLYCTSRTIAWPYEEYKPRLGAVESGTRRMYL
                     HLYYDDERAMEERTAFNILLDSLEAELKEGIRNPEHETLYQKYYEVTQTPVRGVTLNS
                     KQKAIDKVERNYGYFGLLSNDIKDPLEALRIYRTKDLVEKAIANLKERLSAR"
BASE COUNT          611 a          457 c          466 g          559 t
ORIGIN      
        1 aatccgtagg tcgctggttc aagtccagca gggcccaaat tcaaattgct tttatgtttg
       61 tttttatttg cagtttccac tttccttctt ctttcagccg gtattaagat tacggtagca
      121 agggcgaaaa cgatatttcg acaggattat taggattttg caggatttgc aggataataa
      181 ctatggcgat gcgatctttt tctttatata gcgatgcacc gaacgatata aaggaattgc
      241 tcttaaatgg gaagaggatc gacgattttc ttccgccacc ttatcaatta gtgaagcgag
      301 taccgaaggt caaagtgaca attgccttga atcagcagag tttagagttt ttcaagaaag
      361 ctgaggtaac agcaagccaa acattggacc caggcgaagg ataagactct attggaacaa
      421 tataccccgc aacttttatg gaaaactatc atagctattt ttgcgtaact ggatctatgc
      481 atagagggca cctggtcgcc atcataaagg atcgaaaatc gattcatcat tacgccttag
      541 ttacctaata cctaacagca tcgtccactt ttctgagata ttccttcaga gcccttaatt
      601 gtcctaagca cggcggtact ttttcgttct gcaacaccgt gtcgtagatc ttgaattccc
      661 gcgaaattaa acctaggaat agttatttct tggaattaga ataagataat ctcttggcag
      721 attatagtgc tagctataat atataactag tgaggtaagt atgccaaggg gaacacaaat
      781 tatcaatggg gtcgaatatg tctttgaaac aaaggcaaaa tggaatccgg ataaaaaata
      841 tggtacgcat agcagacgat atatcggcaa aatggtcgac ggagtctttg ttccaaataa
      901 acgctatgag ctcgaagagg agctaaaaga agcaaaaaag ccagggcatg tacccgtaac
      961 aatttcagag cgccttttct gtggtgcgac tatgctcctc gatagcatcg gagaaaagct
     1021 cggtattaga gaagatcttg agaaatgctt tcctcaggac tacaagaaga ttctctccct
     1081 tgcctatttt ctcctgcttg aggatcatag cccattaagt aggtttccta gatggacgat
     1141 gctgcatcga catccctatg gaaagattat ctcctcaccg cgttcgagcg agctttttgc
     1201 agctcttgat gaggatagcc aacagcgctt ttttcatctt caaatgcagc gaagaatgga
     1261 acatgagtac cttgcctatg atatcacctc gatctcttcc tactctgagc tcataaggtt
     1321 cgtgaagtat gggaagaata aggagcatga tgccctcccg cagatcaacc tcgccctcct
     1381 ttacggagag tcctcacgac ttcctgtgta ctatcgcagc ctaccaggaa atatcagcga
     1441 tgtaacaacc gtaaaacggc ttatcgatga gctagcttat ctctccattg agaaggtgaa
     1501 gctcgtactc gatcgaggct tctacagtga agagaatgtg aatggactac tctcgcacca
     1561 ctataagttt ctcatttcgc tgaagacatc cctgcgcctt gtgcaaacga tgcttgatga
     1621 agtgcgtgaa tctctgccca caagacagta ctataattca aactttaggc tgtactgtac
     1681 ctcgaggacg attgcatggc cctatgagga gtataagcct cgattagggg cagtggagag
     1741 tggtactcgc cggatgtatc tccatctcta ttacgacgat gagcgggcta tggaagaaag
     1801 gacagccttt aatatactgc ttgatagcct cgaagcagag ctcaaagaag gcattcggaa
     1861 tcctgagcat gagacactct accaaaagta ttacgaggtt acccagactc ctgtacgagg
     1921 agtaacgctc aattccaagc agaaggcgat cgataaggtt gagcgaaact atggctactt
     1981 tggcctcctc tccaatgata tcaaagaccc tctcgaagca ctcagaatct atcgcacgaa
     2041 ggatcttgtc gagaaagcga tagccaacct taaggagagg ctctctgcta gga
//
LOCUS       DAOOWY010000206         2055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_54469_length_2055_cov_11.437500, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000206 DAOOWY010000000
VERSION     DAOOWY010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54469_length_2055_cov_11.437500"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PK105_09180"
     CDS             <1..1037
                     /locus_tag="PK105_09180"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOU61931.1"
                     /translation="AKVKVGVAGYGVIGQRLADGVALQQDMELVGVADVAVTLSVRAL
                     REKGMPYKFFLAAPEKREEFEKANIPLSGSLEDLIQQVDVMLDATSAGVGAKNRLLYE
                     KYSKKGIFQGGEKNSVADVFFHGYANYEKGIGAQFLKLTSCNTTGLIRAVDCIDRKVG
                     LEKVAITIIRRVADPGDYHRGLTNALQIDHAPSHQALDLMTIMPHVDATGILVHTPVT
                     HGHIITVVATPKKSVTPENVIEFFNEHPRIRVVSIAEGFLGNASLFRYARDLGNPRGD
                     MYEIAAWKDSVVMSGKDVMFAINIPQEAVVIPENIDAVRACMRMQLDRIEATDLTNNY
                     LGIGKWRQLS"
     gene            1083..>2055
                     /locus_tag="PK105_09185"
     CDS             1083..>2055
                     /locus_tag="PK105_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU61932.1"
                     /translation="MEGPRLKIKPFTELELTGKTVIFRPDINSPIDPKTKRIVNTNRI
                     EKTVPTLNSLLEQGAKVALIAHQGDTLDYQNLIPLAEHAEILSRLTGRRVSYIDDVCG
                     PAAQAAVKALLPGEAIILGNLRYLTEEISTFETVVKLTPQEMTSAWLVRSLAPLGDYY
                     VNDAFAAAHRNSPSMVAFQELLPSAGGFQLMDEYTALKSVKDSPRRPCVYILGGAKIS
                     DAFDMMRKVLTDGSADYILTAGITGIVMHIGRGVDFGPIVQKFLIDHGLDVFIPEAKA
                     LLREFGERYVLPVDLAYDENAGLSQANASDIANASAFPEQKRAPTSTS"
BASE COUNT          543 a          517 c          513 g          482 t
ORIGIN      
        1 tggcaaaagt caaggttggt gtagcaggat atggtgtgat cggtcaacgc cttgcagatg
       61 gcgttgcatt gcagcaagac atggagctag ttggtgttgc cgatgttgcg gttacgctgt
      121 cggtgcgagc attgcgggaa aaaggcatgc cctacaaatt ctttcttgcg gctcccgaaa
      181 aacgcgagga attcgaaaag gcaaatattc ccctttcagg aagccttgag gatcttattc
      241 agcaggtcga tgtcatgctc gatgcgacaa gcgcgggcgt tggagccaag aatcgccttc
      301 tctacgaaaa atacagcaaa aagggcattt tccagggcgg cgagaagaat tcggttgccg
      361 atgtgttctt ccatggttat gcaaactatg aaaaaggcat aggcgcccaa tttctcaaat
      421 tgacgagctg caatactaca ggcctaatcc gcgctgtcga ctgcatagat cgcaaggttg
      481 gcctcgaaaa agtcgcaatt accattatcc gccgtgtcgc cgatccaggc gattaccacc
      541 gcggccttac gaatgcccta caaattgatc acgccccaag tcaccaggct ctcgacctca
      601 tgactatcat gccgcatgtc gatgcaacgg gcatcctcgt acatactccc gtgacgcacg
      661 gccacatcat cacggttgta gcaacgccta aaaaatctgt tacgcccgag aatgtcatcg
      721 aattcttcaa cgaacaccct cgaatccgcg ttgtatctat tgcagagggt ttccttggca
      781 atgcgagcct cttccgctat gcgcgcgatc ttggtaatcc gcgcggtgat atgtatgaga
      841 tagccgcttg gaaagattcg gttgttatga gtggaaagga cgttatgttt gccataaata
      901 ttcctcagga agccgttgta attccagaaa atatagatgc tgtgcgcgct tgcatgcgca
      961 tgcagctcga ccgaatagaa gctacagacc tgaccaacaa ttatctcggc atcggcaagt
     1021 ggcgacaact tagctgacca aaactcagac cgggctaaga taggataaag gagaagagct
     1081 atatggaagg acctcgactt aagataaaac catttactga actcgaacta accggaaaga
     1141 ccgttatctt cagaccagat atcaattctc caatcgatcc aaagacaaag cgcattgtca
     1201 atacaaatcg aatagagaaa acagttccga ccctcaattc tctactagag cagggggcca
     1261 aggttgccct gatcgcacac caaggagaca cgctcgatta ccagaatcta atcccgctag
     1321 ccgagcatgc tgagatcctt tcaaggctca caggtcggcg ggtttcatat atcgatgatg
     1381 tctgcgggcc ggcggcacag gcagctgtaa aagcgctatt gccaggtgag gcgataatcc
     1441 ttggaaactt gcgctatctc acggaggaaa taagcacctt tgaaacagtg gtcaaactca
     1501 ctccacaaga gatgaccagt gcatggctcg tgcgcagcct tgctcctctg ggcgattact
     1561 atgtaaacga tgcattcgct gcggctcacc gtaattcgcc atctatggta gctttccagg
     1621 aactcctgcc gagtgcggga ggattccagc tcatggatga gtacacggcc ctcaaatccg
     1681 ttaaggatag tccgcgccgc ccatgtgtct acatactcgg tggcgcaaaa atatctgatg
     1741 cattcgatat gatgcggaag gttttgaccg atggaagtgc agattatatt ctaaccgcag
     1801 ggatcacggg cattgttatg cacatcgggc gcggagtcga ttttggcccc attgtccaaa
     1861 aatttctgat cgatcatgga ttggatgtct tcattcccga agcaaaggcc ttgctccgag
     1921 aatttggcga gcgctatgtg cttcctgtcg acctcgccta tgatgaaaat gccggtttgt
     1981 ctcaggctaa tgcatcggat attgcaaacg cttcagcttt tccggagcaa aagcgcgctc
     2041 ccacatcgac atcga
//
LOCUS       DAOOWY010000207         2042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_54947_length_2042_cov_11.448918, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000207 DAOOWY010000000
VERSION     DAOOWY010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54947_length_2042_cov_11.448918"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1744
                     /gene="malQ"
                     /locus_tag="PK105_09190"
     CDS             170..1744
                     /gene="malQ"
                     /locus_tag="PK105_09190"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOU61933.1"
                     /translation="MKIPRSGGVLLHPISLGSPWPCGTMGEEARFFVDFCVKSGLGWW
                     QVLPLGPTAFGDSPYQSSSAFAGNPYLIDPQELWAKGLLSSKEIESYRWKKGNKSRPS
                     SASSEAESSILPSLPEDLEYVDYGRLFTSNARLMDAAWLRYQAKARDEEAAAFDEFCK
                     ANMFWLDDYALFAAIKEKEQHRPWYEWPKEFKSRDPNALARFEKGNKELISRLKLTQF
                     LFFEQWIALKRYANRKGLKLIGDVPIFAAWDSADVWAHRELFQLDDMGRPIRVAGVPP
                     DYFTATGQLWGNPLYDWQAHERDAYAWWIERVRHLLKLVDALRIDHFRGFEAYWAVPF
                     EEPTAKHGRWERGPGHRLFDALKCALGELPIIAEDLGFITPEVVELRDSLGFPGMRIL
                     QFAFELEEDNPDYPHNYPQHCIAYTGTHDNDTARGWLTHAPIAAKKRALSYMHAKPST
                     FHQDMVRTVWASPAMLAIAPMQDFLGLGTEARMNFPGTTSGWWRWRMNREDLSPALAR
                     QIQRLSEIYFRTDASD"
     gene            1763..1942
                     /locus_tag="PK105_09195"
     CDS             1763..1942
                     /locus_tag="PK105_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOU61934.1"
                     /translation="MYGDSMTGIQLFDGDIVVFIPGEIHGDGIYVIRIMDDLYVKRVE
                     FDPINRKIRTMHDNP"
BASE COUNT          545 a          456 c          519 g          522 t
ORIGIN      
        1 tagcgtacaa gtctggggga cttggggtcg ccagttcgag tctggcttgc ccgaagagga
       61 gcaagttgtt gcaatgcaat gacttgctct tttttttctt atacacaagg ttcgtagcag
      121 tattgacaga ataccctttg tagcatacat catgtaaatc ggtttagtta tgaaaatacc
      181 aagaagcggc ggcgtgctcc tccatcctat ttccctgggc agcccatggc cttgcgggac
      241 catgggtgag gaggcgcgtt tctttgtgga cttttgcgta aaatcgggtc tcggatggtg
      301 gcaggttctt ccattaggcc ctacggcatt cggagattcg ccctaccaaa gttcatcggc
      361 atttgcggga aatccttact tgatcgatcc acaagagctc tgggctaagg gattgttatc
      421 tagcaaggaa attgaatcct atcgctggaa aaaagggaac aaaagccggc cttcttctgc
      481 cagctctgaa gcagagagca gtatcttgcc aagcttgcct gaagatctag aatatgtgga
      541 ttatgggcgg ctttttacat cgaatgcaag gctcatggat gcagcctggt tacgctatca
      601 agccaaagcc agggatgaag aagcggcagc cttcgatgaa ttctgtaaag caaatatgtt
      661 ctggctcgat gattatgcac tttttgcagc aatcaaagaa aaagaacaac accgaccatg
      721 gtatgaatgg ccaaaagaat tcaagtctag agatcctaat gctcttgctc gattcgaaaa
      781 aggcaataaa gagcttattt cccgcttaaa actcacccag ttcctattct ttgagcagtg
      841 gatagcgctg aagcgatatg ccaacagaaa gggtttgaaa ctgataggcg atgttcccat
      901 ctttgccgct tgggattcag cggatgtttg ggcgcatcgc gagctcttcc aactcgatga
      961 tatgggtaga ccaatcaggg tagcgggtgt tcctcccgat tattttacag caactggtca
     1021 gctatgggga aatccgcttt atgattggca agcgcacgag cgagatgctt atgcatggtg
     1081 gatagaacgc gtgcggcatt tacttaagct cgtggatgcc cttagaatag atcactttcg
     1141 aggattcgag gcatattggg ctgttccatt tgaagaaccc actgcgaagc atgggcgatg
     1201 ggaaagaggc ccagggcata gactcttcga tgcgctcaaa tgcgcactag gagaattgcc
     1261 cataattgcg gaggatttgg ggttcatcac ccctgaggtt gtcgaactgc gcgatagtct
     1321 gggttttcca ggcatgagaa ttttgcagtt tgcctttgag ttggaagaag acaatccgga
     1381 ttatccccat aattatcctc aacactgcat tgcctacacc ggcactcacg acaacgacac
     1441 cgccagaggc tggctaactc atgcacctat cgccgcaaaa aaacgcgcgc tttcgtatat
     1501 gcacgcaaaa cccagcacat tccaccagga catggtacgt actgtgtggg caagcccggc
     1561 tatgctcgct atcgctccaa tgcaggattt tcttggcctt ggtacagaag cgcgcatgaa
     1621 ttttccagga accacgagcg gctggtggcg ttggcgcatg aacagggaag atctaagccc
     1681 agcgctcgcc cggcagatac aaaggctttc cgaaatctat ttcagaacgg acgcctcgga
     1741 ctgaaaaagc agagacagcg cagtgtacgg agactcaatg acaggcattc agcttttcga
     1801 tggagatatt gtcgtcttta taccaggtga gatacacgga gatggaatct acgttattag
     1861 aattatggac gacctctatg taaaacgtgt cgagtttgat cctatcaata gaaaaatcag
     1921 gactatgcat gataatccat gatatcccga ccgtatcgaa tcggctgacg gacagagtta
     1981 attatatttt gctcttattg attagaaaat gattttttca ttggcaatga aatatgataa
     2041 tg
//
LOCUS       DAOOWY010000208         2013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_56055_length_2013_cov_14.868744, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000208 DAOOWY010000000
VERSION     DAOOWY010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56055_length_2013_cov_14.868744"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PK105_09200"
     CDS             <1..840
                     /locus_tag="PK105_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU61935.1"
                     /translation="LAAMPIAVENQMMSITATTSYEYAVQFFPWTVSWFPPTLDRLPP
                     LVAGWAKQFSDLKQVVQFIEPYGPWPGMADAHSKALTSLNIKELKAIEVPTDAVTFGP
                     LVVRALDQKPDGIILVCNAEKAAKIIKELKSRGWTKMNHILVFSSADDAQLYSTGGAD
                     LNGVQVYNYLNLEVSNPRWDKFKAAFAKDHNGQMPFSLSPNYYDALYMIKEAIEKTGV
                     TGDPKKLKEERKKIADYCANVKGFKGLLYEWDMKDGVPTNKPSYIFEIQNGVKQFVLE
                     VRP"
     gene            complement(946..2004)
                     /locus_tag="PK105_09205"
     CDS             complement(946..2004)
                     /locus_tag="PK105_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOU61936.1"
                     /translation="MYDSWCFFAHPNSSNQAQTFERQKYPIGCSSFSAWGEATRDGTF
                     IFGKNMDNLNLPGILEGRMLVVCDPDNGLGHVNVSHPGMIAIDGGINENGIEMMTQYS
                     PSIYETMRGCGIAVLSRLILQNASRVDDALNILTVYPRCTGINYHVGDAKAQRAIVIE
                     TNAKQIAFRRPYKQDILWTTNHYNCYPGWMGYQGENLVEGQKLAFCLKDVKSIESWQE
                     SLKDKSNPYLSATGRFRQYEKLLSELYGEITMESGIEILSDRHNPDTGELRDWETAPK
                     ARNDGNTIAFWAAPTTFAEKVKFYKSNLETSIVAHTGNLWSMIATPLNGDFRIAMSDF
                     PAQRGPYVHFNLFEELNR"
BASE COUNT          530 a          452 c          450 g          581 t
ORIGIN      
        1 ttggcagcca tgccgatagc agtagaaaat cagatgatgt ccataactgc gacgacttcc
       61 tatgaatatg ccgtacagtt tttcccatgg actgtttcat ggtttccgcc gacactggat
      121 agattgccac cattggtcgc tggctgggca aagcaattca gcgatctaaa gcaggttgtg
      181 cagtttatcg agccctatgg tccatggcca ggcatggcag atgcacatag caaagcgctc
      241 accagtctta atattaagga attgaaagca atcgaggttc ctactgacgc tgtcaccttt
      301 ggcccacttg tggtaagggc tctcgatcag aaacctgatg gaataattct cgtttgcaat
      361 gcagagaaag ctgcaaagat catcaaggaa ctaaagagcc gcggctggac aaagatgaac
      421 catatcttgg ttttctccag tgctgatgat gcacagctat actctacagg cggagcagat
      481 ttgaatggtg ttcaggtcta taactacctc aacctagaag tatccaatcc tcgctgggac
      541 aaattcaaag cagcttttgc gaaagatcac aatggccaga tgcccttctc gctatcgccg
      601 aattactatg acgcactcta tatgatcaag gaagcgatcg agaagacagg agtcacagga
      661 gatccaaaga agctcaaaga agagcgcaag aagattgccg attactgtgc aaatgtaaaa
      721 ggctttaagg gtctactcta tgaatgggac atgaaggatg gagtgcctac caataagcct
      781 tcttatatat tcgaaattca gaatggggta aagcaattcg tacttgaggt gaggccataa
      841 gaaaagtagt ttataagata gtgtgaatcg atcaagaagg ggcgccgcgc atgcgcggcg
      901 ccccttttgt aagattatgg ctttttcaga atctctccgc ttttatcacc tattcaattc
      961 ctcgaagaga ttaaaatgca catatggtcc cctctgggca gggaaatcgc tcattgcaat
     1021 tctaaagtca ccattcagcg gagtggcaat catgctccat aaattccccg tatgggctac
     1081 aatagatgtt tcaagattgc ttttatagaa cttgaccttt tcggcaaagg tggtaggggc
     1141 agcccaaaaa gcaatagtat tgccatcatt tcgcgctttg ggagctgtct cccaatctcg
     1201 tagttcgccg gtatctggat tatggcgatc gctcagtatt tctataccgc tttccatggt
     1261 gatttcccca tagagttcgc taagcaattt ttcgtattgc ctaaaccttc ccgttgcgga
     1321 aaggtaagga tttgatttgt ctttgaggct ctcttgccat gattcgatac tctttacgtc
     1381 cttgaggcag aatgcgagct tttgcccttc tacgagattc tctccttgat atcccatcca
     1441 acctggatag cagttgtaat gatttgttgt ccataagata tcttgcttat aaggtcttct
     1501 aaaggctatt tgctttgcgt ttgtttcgat cacaatggct ctttgagcct ttgcatctcc
     1561 tacatggtag ttaattcctg tacatcttgg atatactgtg aggatattca gagcatcgtc
     1621 tactctgctt gcattctgca atatcaagcg tgagagcaca gcaatgccac atcctcgcat
     1681 tgtttcatag atggaggggc tgtattgggt catcatttca atgccgttct cgtttatgcc
     1741 tccatctatc gcaatcattc cagggtgtga gacatttaca tgccctaatc cattgtcagg
     1801 atcgcatacc accaacattc ttccttcaag tatgcccggc agatttaggt tgtccatgtt
     1861 tttcccgaat atgaatgttc cgtctcgagt agcttctccc caagcagaga aacttgagca
     1921 tccaatagga tatttttgcc tctcgaaggt ttgagcttgg ttgctgctat ttgggtgtgc
     1981 gaaaaagcac caagaatcat acatagtatt cca
//
LOCUS       DAOOWY010000209         2011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_56136_length_2011_cov_24.412065, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000209 DAOOWY010000000
VERSION     DAOOWY010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56136_length_2011_cov_24.412065"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK105_09210"
     CDS             complement(<1..406)
                     /locus_tag="PK105_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61937.1"
                     /translation="MIKLKKSATLANDARPGLLDRCILTILVLFIVLTSLPSQGILTS
                     STGNGLEEIRPTIASSTEKLDVVGSVDRSPPEIPGLIPIASFDEGRSYRTAVGSYSVL
                     SLKGSWREMGRQYGALLSKELKEFYAAINADLQ"
     gene            complement(509..1156)
                     /locus_tag="PK105_09215"
     CDS             complement(509..1156)
                     /locus_tag="PK105_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOU61938.1"
                     /translation="MSVITFSREPCSNGTLIAQSVAKKLGYRLATKKSIEEVLLQYGF
                     VDVAEAYDSMPNFWTRFDDRTHQIVTMFDRVTKALAKLGNVVIIGRGAFRVLAPYRDV
                     LNVRIKSPFPYRVATFMNRYKVSNQHDAEVAILEADRIRSAFLEVHYGIKWDSMTNFD
                     LVIDTSKIPHSSAIPMIIEANKAVMKAICEPRESASSIEVDDILLEATRKVIENC"
     gene            1314..>2011
                     /locus_tag="PK105_09220"
     CDS             1314..>2011
                     /locus_tag="PK105_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOU61939.1"
                     /translation="MSQTERIFYIDRMIREHGGVSARQICDKFEVSSRQAKRDIEYLR
                     DRLDAPIVWSHRRLRYEYSETWNGLRFADETSFFAFAFLRAILDRYAYVPVVSEETTK
                     LLKEKIGGRYISISDKVRYELPDMETIDGEAAFTMCQALLNNSALDISYIDAKGESSQ
                     RRIIPLRLINYAGKWYCIAFDSKSMELRTFSISRMDNVRAAGGLSASAESSRALSKGK
                     QSSENLPKGAPNFP"
BASE COUNT          566 a          497 c          454 g          494 t
ORIGIN      
        1 cctgcagatc ggcattgatc gccgcataga attctttgag ctcctttgaa agcagcgctc
       61 catattgtcg acccatttcg cgccaacttc ccttgaggct caaaacagaa taagagccaa
      121 ccgcggttct atagcttctt ccttcatcaa atgatgctat cggaattagc ccagggattt
      181 ccgggggcga cctatcgaca gagccaacaa catccagctt ttccgtcgag gaagctatgg
      241 tggggcggat ttcctcaagc ccgttacccg tcgaggaagt caaaataccc tgtgaaggta
      301 aagaggtcag cacaatgaac aagaccaaaa tcgttaagat acagcgatca agaagccccg
      361 gtcgcgcgtc attcgctaag gttgcagatt ttttcagctt tatcattatt ttctccctga
      421 aacaccgacc aattagagag acaaatcaga aaactatatc ctggcttttc tgtcaaagac
      481 atactcgcaa aaaatcccta aatccatctt aacaattctc gatcactttg cgcgtcgcct
      541 ctaagagaat gtcgtctacc tcgatggaag atgcagattc tctgggttcg cagatagctt
      601 tcataacagc cttattagcc tcgatgatca ttggtatggc cgagctatgc ggaattttgg
      661 acgtatcgat gacgagatcg aaattggtca ttgaatccca ctttatgcca tagtgaacct
      721 caagaaaggc cgatcttatt ctgtctgcct cgagtatagc cacctctgcg tcgtgctgat
      781 ttgagacctt gtaacgattc atgaatgtgg ccaccctata cggaaatggg ctcttgattc
      841 gcacattcag cacatcgcgg taaggagcaa gcaccctaaa tgcacctctg ccaataatca
      901 ctacattgcc cagcttggcc aacgcttttg taacgcgatc gaacatggtg acaatctgat
      961 gagtcctatc atcgaagcgt gtccagaaat tgggcatgct atcgtaggct tccgccacat
     1021 cgacaaaccc atattgaagc agtacttctt cgatcgattt cttggtggca agacgatagc
     1081 cgagtttttt agctacagat tgagcaataa gagtaccgtt agaacaaggc tccctagaaa
     1141 aggttataac gctcatcgga tacctccttc tctaattata gtattggcta aaagataagc
     1201 gaagtcaatg aaagatatgt acagaaaatg acgataataa agtcatatgg gacactctgt
     1261 gtccctcgcc aggagtataa tcagaaataa aaaaatagaa ggcggaggcc tccatgagcc
     1321 aaaccgagag aattttctac atcgatcgca tgataaggga gcatggaggc gtctccgccc
     1381 gccaaatatg cgataaattc gaggtatctt cgcgccaagc caagcgcgat atagaatatt
     1441 tgcgcgatag gctcgacgca cccattgtgt ggtcgcatcg cagactgcgc tatgaatatt
     1501 cagaaacatg gaatggcctt cgctttgcag acgagacatc gttctttgct tttgcgttcc
     1561 tgagagccat tttggaccgc tatgcctatg tcccagttgt ctcggaagag acaaccaaac
     1621 tcctcaagga aaagataggc gggcgctaca tctcaatatc ggataaagtg cgctatgagc
     1681 ttcccgacat ggaaaccatc gacggagaag cagcctttac gatgtgccag gccctgctca
     1741 acaacagcgc gctcgatatt tcctacatcg atgcaaaagg cgaatcgagc caacgaagaa
     1801 ttatcccctt gaggctcatc aactatgccg gcaagtggta ttgcatcgca ttcgattcga
     1861 aatccatgga gctgaggaca ttttctatat ctcgaatgga caatgtccga gctgcgggcg
     1921 gattgtctgc aagcgccgag tcatcaagag cgctgtctaa aggcaagcaa tccagcgaga
     1981 atttgcctaa gggcgcgccc aactttccta t
//
LOCUS       DAOOWY010000210         2009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_56212_length_2009_cov_7.411464, whole genome shotgun sequence.
ACCESSION   DAOOWY010000210 DAOOWY010000000
VERSION     DAOOWY010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56212_length_2009_cov_7.411464"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1168
                     /locus_tag="PK105_09225"
     CDS             26..1168
                     /locus_tag="PK105_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU61940.1"
                     /translation="MSAKKKFSTFLSRYAVVIIFLFVTIVATPPSGLSIRYILQELIT
                     RLGRNSFLVLALLLPIYAGMGLNFGMTLGAMAGQIGLIFAVNYSIAGWQGLVFALLVG
                     LPISVLLGWICGLVMNRAKGREMVTGYILAFFMNGIYQFFVMYMLGSVVPMGNKAIVL
                     SRGYGIRNTLNLESVRQSLDNILVLRFAGFTFPVVTFIVIAVLCVFIIWFKKTKLGQD
                     MRAVGQDMAVAESAGIPVERTRIIAIIISTVLACAGQIIFLQNMGNLATYNAHDQTGF
                     FAVASILVGGASVTSANIANVFIGVMLLHAMFVVSPLAGQNLFGSAMIGEYFRQFIGY
                     GVIALSLVLYAWRSKKAAAEARISLRGANNNHKDKIEVTTKNGGQA"
     gene            1165..1692
                     /locus_tag="PK105_09230"
     CDS             1165..1692
                     /locus_tag="PK105_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61941.1"
                     /translation="MNEPTGEKSIGNAKNTKKRRLIIRSGLLVVYVLLIAFMIHSGRR
                     HTILIDNKDAHDDSYIGIDGMEVSIDNKEPLEYYRGDRDKAVVQGQKHTIKVEIFDDG
                     RKIEESFTVPLWTDMVIIYVPRLVNGIEPWLEPFTIAEQIEESQESWSPDDEMSFESM
                     GSPQLEGVENTTPTP"
     gene            1829..>2009
                     /locus_tag="PK105_09235"
     CDS             1829..>2009
                     /locus_tag="PK105_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOU61942.1"
                     /translation="MRKLVAVILVLAFLVVGADVFAQAKAKFHIGIATETVSQAEDDL
                     RGAEQLIKEYGSVKDG"
BASE COUNT          530 a          418 c          508 g          553 t
ORIGIN      
        1 tcacgcgcaa gatgaaggtg tcaaaatgag cgcaaagaaa aaattctcga catttctatc
       61 acgatatgct gtagtcatta tttttctttt tgtaacgata gtggcaactc cgccatcagg
      121 gctatctatt aggtatatat tacaggagct cattacaagg ctagggagaa attccttcct
      181 cgtattggca ctactattgc cgatatacgc cggtatgggg ctcaatttcg gcatgaccct
      241 gggtgctatg gcaggccaga tagggcttat atttgcagtg aactacagca tagctggatg
      301 gcaggggctt gtgtttgctc ttcttgtggg cttgcctatc tcggtgctgc ttggctggat
      361 ctgcggtctt gtcatgaaca gagcaaaagg acgcgagatg gtaacaggct acatccttgc
      421 gttttttatg aacgggatct atcagttctt tgttatgtac atgctgggct cggtcgtacc
      481 gatggggaat aaggctattg tcctgtcgcg cggttatggc attcgcaata ccctaaatct
      541 tgaatcagtg cgtcaatcgc tggacaatat ccttgtattg cgcttcgcag gatttacttt
      601 cccagtggtg actttcattg taatagccgt actttgtgtt tttataattt ggtttaagaa
      661 gactaagctg ggacaagaca tgcgagcggt gggtcaggat atggcagtgg ccgaatcagc
      721 tggcatccct gttgaaagga cgcgtattat agcgattatc atctcgacgg tgcttgcttg
      781 tgccggacaa attatcttcc ttcaaaatat ggggaacctt gctacctata atgcacacga
      841 ccagacaggt ttctttgccg ttgcttctat attggttggc ggtgcatcgg ttactagcgc
      901 aaatatcgct aacgttttca taggtgttat gctcttacat gctatgtttg ttgtatcgcc
      961 acttgctggg cagaatcttt ttggctcggc catgattgga gaatatttcc gccaattcat
     1021 cggttatggt gtgatagcgc tttcgctcgt gctctatgct tggagatcta agaaggctgc
     1081 cgcagaggct cgaatcagtc tacgtggagc gaacaataat cacaaagaca agatcgaggt
     1141 cacgacgaaa aatggagggc aggcatgaat gaaccaacgg gcgagaaaag tatcggtaat
     1201 gcaaagaata caaagaaacg tcgcctcata attcgttcgg gccttctcgt ggtttatgtg
     1261 cttctcatcg cttttatgat ccactctggc aggcgacaca ccatactcat cgacaacaag
     1321 gatgcgcatg atgactccta cattggcata gatggaatgg aggtgtcgat cgataataaa
     1381 gagcccctgg aatattatcg cggagatcgc gataaagcag tggtccaggg gcaaaagcac
     1441 acaatcaagg ttgagatatt cgacgacggg cgcaaaatcg aagagtcttt tactgtccct
     1501 ctttggaccg atatggtaat aatttatgta cctaggctgg ttaatggaat tgagccatgg
     1561 ctagagccat ttaccatagc tgaacaaatt gaggaatctc aagaatcgtg gtcgcccgat
     1621 gatgaaatga gctttgaaag catgggtagc ccacagctag aaggcgtaga aaacactact
     1681 ccgactccat aaaaaattcc aaaaacgagc tctcaataat tcatggctca tggagtcgtg
     1741 aattttcgct ccaaatgcga agaggcttga tttgtatttt caaggtgtag actaattctg
     1801 catcttcaat atcaaataag gaggacgtat gagaaagctt gtagcagtta ttctagtact
     1861 agcttttcta gtggtgggtg ccgatgtatt cgcccaggcc aaggccaaat tccatatcgg
     1921 catagctacc gagacagtct ctcaggctga ggacgacctt cgcggagcgg aacagctcat
     1981 aaaggagtac ggaagcgtca aagacggtg
//
LOCUS       DAOOWY010000211         1979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_57366_length_1979_cov_2.867983, whole genome shotgun sequence.
ACCESSION   DAOOWY010000211 DAOOWY010000000
VERSION     DAOOWY010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57366_length_1979_cov_2.867983"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="PK105_09240"
     CDS             complement(<1..1252)
                     /locus_tag="PK105_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61943.1"
                     /translation="MAQKDSELDFFFGLFKRKKKAIGLSVGLSCLQSLSLAPIALFVN
                     RAIGNAIPEGDMGALIGCFAGIALLALASGAIQLLNRRISIGAIKASIADLRVALVKA
                     QLHGSRKYYTKEDLDLLHSRIVQDTFRVDSMAGALFTQAIPGALISLGLIFVLLRLNL
                     LLGIICICALPVFAAGALLISKRLKGLIRVFHQDYSQYSKGIKFMLESNELIRISTAE
                     LIEEKEQRATIERLKDSHGRALLFSSVFSIGQQQLLIVGAALILLVGGYFVMRGNFSV
                     GQLVAFYAALGMLQGYVGSVVNAVPTLVEGHESLRVLQAIIDTARQEAQEGQEDRASQ
                     ESQKYQESQKSLESQTSQASQEGSHKGLDHEIGKSIEFAHVSFKYPITNHGAEPRIKR
                     GADTDAGAEPKEDALKDEAPPLLSD"
     gene            1365..>1979
                     /locus_tag="PK105_09245"
     CDS             1365..>1979
                     /locus_tag="PK105_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61944.1"
                     /translation="MSTYYYHAFDMQIQSDFELSLPSIEEGGSTSHILEIIEGNVPLP
                     AELPFVLGSIRYGMEGNCIIFDIPWSARYRIEGSSRIVVEPIPNVGIEWIDLYITNFL
                     LTIVLSMRGIATLHGSAISRDGKGIIFLGKKGAGKSTIAAKMALSGYEMLCDDAIPVS
                     PDGMIFPGIPYPKLLSDAFSQLIGQPSNESRLFDGVNKYRVELPS"
BASE COUNT          510 a          503 c          459 g          507 t
ORIGIN      
        1 tatcgctgag gagcggaggc gcctcgtctt ttagcgcatc ttctttgggc tcggctcccg
       61 catctgtgtc agcgccccgt tttatgcgag gctcggcgcc atgatttgta attggatact
      121 taaaagagac atgcgcaaac tcgatggatt tgccgatctc gtggtcgagg cccttgtgcg
      181 agccctcttg gctagcctgg cttgtttggc tttctaggct tttttggctt tcttggtatt
      241 tttgactttc ctgacttgcc cgatcttctt ggccttcctg agcttcctgg cgcgcggtat
      301 cgataatggc ttgtagcacg cgcagcgatt catggccttc aacgagggtc ggaacagcat
      361 tcaccaccga ccccacatag ccctgcagca tgccgagcgc cgcatagaac gccaccagct
      421 gacccacaga aaagtttccc cgcatgacaa aatacccacc taccagtaag atgagcgccg
      481 cccccactat gagcagctgc tgctgcccaa tactgaaaac cgaagagaat agcagcgccc
      541 tgccatgcga atccttgagc cgctctatgg tagcgcgctg ctctttttct tcgatcaatt
      601 cagcggtcga tattcttatt agctcgttgc tttcgagcat gaactttatg cccttgctgt
      661 actgcgaata atcctgatga aataccctta taagcccttt taggcgcttg cttatcaaaa
      721 gagcccctgc cgcaaagaca ggcaatgcgc aaatgcaaat aatgccgagc aacaaattca
      781 gccttagcaa gacaaaaatc agccccaagg atatcaaagc gcctggtatt gcttgggtga
      841 agagcgcccc tgccatagaa tcgactcgaa aggtatcttg gactatcctc gaatgcaaca
      901 agtctaaatc ttcctttgta taatactttc tggaaccgtg gagctgagcc tttacgagcg
      961 ccacgcgcag gtcagcaatg cttgccttga ttgcgccaat cgagatgcgc cgattgagaa
     1021 gctgtattgc accgctcgcc aaagcaagaa gcgcaattcc agcaaagcag cctataagcg
     1081 cccccatatc gccttccgga atggcgtttc caattgcgcg gttaacaaaa agcgcaatcg
     1141 gcgccaagga cagcgactgc aagcacgaaa gcccaaccga aagtcctatt gccttttttt
     1201 ttcttttaaa gagtccaaag aaaaaatcca attccgaatc tttttgtgcc atttggttct
     1261 gactgattat gaatgaggcg cgttaaaact gcaagaagca aaaattgtgc tcaacatatt
     1321 gagccataat gccgaaaaaa gtaatataaa ttagggatat tggtgtgagc acgtactatt
     1381 accatgcatt cgatatgcag atacaatctg actttgaact ttctcttcct tctatagagg
     1441 aagggggatc cacgagccat atcctcgaaa taattgaagg aaatgtacca ttgcctgcgg
     1501 agcttccttt tgttcttgga agcattcgat atggaatgga aggaaactgt attatattcg
     1561 atattccttg gtccgcgcgc tatagaatag aaggaagctc tagaatcgtg gtagagccca
     1621 ttccaaatgt cggaatcgaa tggatagatc tatatattac aaacttcttg ctcacgatag
     1681 tcttgtcaat gcgaggcatt gctactctcc atgggagtgc gatatcgaga gacggcaaag
     1741 gcataatctt tcttggcaag aaaggcgccg gcaagtcgac catagccgca aaaatggcat
     1801 tatctggcta tgaaatgctc tgcgatgacg ccattccggt tagcccagac ggaatgattt
     1861 tcccagggat tccctatcct aagcttctct cggatgcctt tagtcaactg ataggccagc
     1921 cgtccaacga aagccgactc ttcgatgggg ttaacaagta tcgcgtggag ctgccttcg
//
LOCUS       DAOOWY010000212         1972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_57650_length_1972_cov_9.884194, whole genome shotgun sequence.
ACCESSION   DAOOWY010000212 DAOOWY010000000
VERSION     DAOOWY010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57650_length_1972_cov_9.884194"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /gene="trmD"
                     /locus_tag="PK105_09250"
     CDS             complement(<1..754)
                     /gene="trmD"
                     /locus_tag="PK105_09250"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOU61945.1"
                     /translation="MRIKVISLFPEIIEAYFSASIMKRAVEKGLISYELVNIRDYAFD
                     RHRKCDDEVFGGGPGMLMKPEPLDRALAAAGSPEMRTIYVTPSGRLFNQVMALDFAHE
                     ENLILLCGRYEGIDQRIIDTRVTDLVSIGDYVLSSGEIAAMVVIDAVYRLIPGVISHE
                     SLSEESFSSGLLEYPQYTRPAKYGNLIVPEVLVNGNHAAIDRWRLKASLVKTLAYRPE
                     LLQNIDLTSELGRLLKEVIDEGGGHGPHTADSS"
     gene            complement(751..1278)
                     /gene="rimM"
                     /locus_tag="PK105_09255"
     CDS             complement(751..1278)
                     /gene="rimM"
                     /locus_tag="PK105_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOU61946.1"
                     /translation="MHERQEPEYLAVARLGAVRGLTGELRLFSYSGEYSHIEDAQEFL
                     LGGREGLRDAKPIRVLRLQKASWGVSIVFEGFETPEKARRLVGRDIFLPRSKASPKKS
                     GEYYIADLVGMTAIADGEEIGVITAVIEGAESDLLEIERDFKKKILVPFRNEFVGKID
                     EERGELEIISPWILE"
     gene            complement(1294..1527)
                     /locus_tag="PK105_09260"
     CDS             complement(1294..1527)
                     /locus_tag="PK105_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOU61947.1"
                     /translation="MERDLVEYIVKSLVDHPESVDIKVVEGEKSTILELRVAESDIGK
                     VIGKNGRIARAIRTILSASASSKSKRVILEILD"
     gene            complement(1542..1793)
                     /gene="rpsP"
                     /locus_tag="PK105_09265"
     CDS             complement(1542..1793)
                     /gene="rpsP"
                     /locus_tag="PK105_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOU61948.1"
                     /translation="MSVRIRLKRFGAKARPYYRIVVMDSRSARDSKTIDEIGYYHPIE
                     AEDKQISINEAKVREWLAKGAQPSDTVRSLLNKKGIQLK"
BASE COUNT          503 a          523 c          369 g          577 t
ORIGIN      
        1 cgcttgaatc tgctgtatga ggtccatgtc ctcccccttc gtctatcact tctttgagca
       61 atctgcctaa ttcactggtc agatcgatat tctgcaaaag ttccggtctg tatgcgagcg
      121 ttttgacaag actcgctttg agtcgccatc ggtctatcgc cgcatggttg ccgtttacca
      181 acacttccgg gacaattaaa ttaccatatt tggcgggcct tgtatactgg ggatactcta
      241 gaaggccact tgaaaaactc tcttccgaaa gagattcatg cgaaataaca ccaggtataa
      301 ggcgatacac cgcatcgatc acaaccatcg ctgctatctc tccgctcgaa agcacataat
      361 ctcctatcga aaccaaatcc gtaactcttg tatcgattat gcgctgatct attccctcat
      421 atcggccgca gagtaaaata agattttcct catgtgcaaa atccaacgcc atcacctgat
      481 tgaaaagcct gcctgacgga gtcacataga tcgtcctcat ttccggagat cccgccgccg
      541 caagagctct atccaaaggc tctggcttca tcagcatgcc tggtccacca ccaaacactt
      601 catcgtcaca tttgcggtga cggtcaaaag catagtctct tatattgact agctcatagg
      661 atatcagccc tttttccacc gcgcgtttca ttatagaagc agagaaataa gcttcaataa
      721 tttctgggaa aagagaaata accttgattc tcattccaga atccatgggc taatgatctc
      781 gagttcgccc ctctcttcgt cgattttccc gacgaactca tttcggaagg gaaccaggat
      841 ctttttttta aagtctctct caatttccaa gagatcgctc tctgctccct ctattaccgc
      901 ggtgatcacc ccaatctctt ctccgtcggc tattgcggtc attccgacaa ggtcggcaat
      961 atagtattct ccggattttt tgggactggc ctttgatcta ggcagaaaaa tatccctccc
     1021 taccaatcgg cgcgcttttt cgggcgtctc aaatccctcg aaaactatgc ttactcccca
     1081 gcttgccttt tgtagacgca aaactcttat gggcttggca tctcttagcc cttctctacc
     1141 acccaagaga aattcttgtg cgtcttctat atgcgaatat tctcccgaat agctgaatag
     1201 cctgagctcg cccgttagac ctcttacggc accaagtctt gccactgcca gatattcagg
     1261 ttcctgcctt tcatgcatga taaaaaatca atctcaatca agaatctcca agatcactcg
     1321 ttttgacttg gacgatgcgg atgcggaaag aatagtacga atcgcacgag ctattctgcc
     1381 attcttgcct atgacttttc ctatatcgct ctccgcaacc cttagctcga gaattgtgga
     1441 tttctctccc tcgactacct tgatatcgac ggattcagga tggtcaacaa gcgatttaac
     1501 aatatattca acaagatcgc gttccatagt gttctcacca ctcattttag ttgaatgcct
     1561 tttttgttca aaagagaccg aacggtgtcc gatggttggg caccttttgc aagccattct
     1621 cttactttgg cttcatttat gcttatctgc ttgtcctcag cttctattgg atgatagtat
     1681 ccaatctcat caatggtctt ggaatcgcga gctgagcggg aatccattac aacaatacga
     1741 taatatggtc tagccttagc gccaaacctt ttgagcctta tacgtacgct caccctaatc
     1801 ctcctgaata caaaaaatgc aatgtgggct tcgccttatc cgcacatccg ttcctctacg
     1861 aagcccgata tatggaacat gtgcggaaga tttatatact atgaatgggc aaagagtcaa
     1921 cgcagtacct tgaccatttt caattttaca tttatggtaa taatgtcaaa at
//
LOCUS       DAOOWY010000213         1959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_58223_length_1959_cov_10.392857, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000213 DAOOWY010000000
VERSION     DAOOWY010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58223_length_1959_cov_10.392857"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /gene="lnt"
                     /locus_tag="PK105_09270"
     CDS             complement(<1..1684)
                     /gene="lnt"
                     /locus_tag="PK105_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOU61949.1"
                     /translation="MNELVKGLYRQNMPRLGNLKNNSTPFWIRLLWAFLAAIFAALAQ
                     PNEIFLYGNWFIGIFCLVPLYMALVDTEKLGEASLIGALFGGLYHALTSYWLFFYKDF
                     AFWTLGTTTIAYAVIYGVALMYGCFLLHHTDGCRPLVFALAWAALEFAKSTGFLGYPW
                     GLLAYSFTGLPIMLQTADLWGVYGISAFLAFCSSATSELILQKTRKMDSVRSCIRWPE
                     ITAATWALILLLYGALIMSRPFPIKGTLRLLLCQQNTDPWTSGENEALYANIRVAKQA
                     LKSIQEEKSAKPDLIVFSETSLRRPYKEFKSWFEENPPEEPLLPFLRIADIPLLTGLP
                     VVWNWETYEASNSVGIIYPTGRMGETYAKIHPVPFAEAIPFWEFSWFRSFFQNVIGLE
                     SGWVMGREEVIFALSSEDKYHSFDEMGSLPTIRFATPICFEDAFSELCRNFIHRGADL
                     LINLTNDSWSRTESAQIQHYAVARLRAIETRRALVRSTNSGVTCVVGADGKNIAELAQ
                     FITTSMVVDVPIYESHFTFYLIFGDWFAWACTAFVGLLFIWAILDTKRKNYVI"
BASE COUNT          597 a          453 c          414 g          495 t
ORIGIN      
        1 gtattacata atttttcctt tttgtatcta gaatcgccca gataaaaagc agcccaacga
       61 aagcagtaca agcccaggca aaccagtctc caaaaatcag atagaaagta aaatgagatt
      121 cataaatagg aacatccacc accatagaag tggtaataaa ctgagccaat tcagcaatat
      181 tctttccgtc tgcgcctacc acacaggtga ctccagagtt agtcgagcgc acaagtgctc
      241 gccttgtctc tattgctcta agccgtgcga cagcataatg ctgaatctga gcactctcag
      301 ttcgagacca tgaatcgttt gtcagattta tcaacaaatc cgcgcctcga tgaatgaaat
      361 ttctacatag ttcggaaaag gcatcctcga agcatatagg cgtagcaaat ctaatggtag
      421 gcaagctgcc catttcatca aaactatgat atttatcttc ggagctcagt gcaaagatta
      481 cttcttctct tcccattacc cagccgcttt caagtccgat tacattttga aaaaaggagc
      541 gaaaccaaga gaattcccag aaaggaatgg cttctgcgaa aggtacagga tgtattttcg
      601 cataagtttc acccattcta cctgtcgggt aaataatacc tactgaattg gatgcctcat
      661 atgtttccca attccagacc accggaagac cggtcagcaa tggaatgtca gcgatccgaa
      721 ggaagggcaa tagaggttct tcgggaggat tctcctcaaa ccaggatttg aactccttgt
      781 aaggccgcct caagctagtt tcgctgaata ctatgagatc gggcttggcg cttttttctt
      841 cctgaattga tttcaaagcc tgtttagcta ctcttatatt cgcgtatagc gcttcattct
      901 ctccagatgt ccatggatct gtattctgtt ggcagagcag aagccgcaat gttcccttga
      961 taggaaaagg ccgagacatt atcaaagcac catagagaag taatatcaaa gcccatgtag
     1021 cggcggttat ttcaggccag cgaatacaac tccttacgga gtccattttt cttgttttct
     1081 gcaagataag ttctgaggtt gccgatgaac aaaaagccaa gaaggcagaa atgccgtaga
     1141 caccccagag gtcggccgtc tgcagcataa tcggaagccc agtaaatgag taagccaaaa
     1201 gtccccaagg atatcccaaa aagccagtcg attttgcaaa ctcgagagct gcccatgcca
     1261 gagcgaatac aagaggcctg cagccatctg tgtgatgcaa gagaaaacac ccatacataa
     1321 gggctactcc atatattacc gcataagcga tagttgtagt ccccagggtc caaaatgcaa
     1381 agtccttata gaaaaagagc caatagcttg tcagtgcatg atagagtcct ccaaatagag
     1441 caccaatcag gcttgcttct ccgagtttct ctgtatcaac aagtgccata tagagtggca
     1501 caaggcaaaa gatgccgata aaccaattcc catacaagaa tatctcgttg ggctgagcaa
     1561 gtgcggcaaa tatggcggct aaaaatgccc atagcaagcg aatccaaaaa ggcgttgaat
     1621 tatttttaag atttccgagc cttggcatat tctgtcggta cagtcctttg actagctcat
     1681 tcataatgaa attagtataa gccaaagatc tctttggcta aagagcaagg gcaaattctt
     1741 tccattgact gttggagcag aactgcatta aattaaagac catgccaaat gcggtctttg
     1801 caggaagctt tgatcctccg acatcaggcc atctcgatgt gatcgagagt gcccttagca
     1861 tattcgacgg gctttatgtc gtcttggcca tcaaccccga aaaagatgga ttgttcacta
     1921 ttgatgagcg tatcgaaatg ctcaatgcca tgaacagat
//
LOCUS       DAOOWY010000214         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_58966_length_1941_cov_38.674973, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000214 DAOOWY010000000
VERSION     DAOOWY010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58966_length_1941_cov_38.674973"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PK105_09275"
     CDS             <1..659
                     /locus_tag="PK105_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOU61950.1"
                     /translation="EVASHIKELIRDEKLKPGEKLPNELELCGLFSVSRPTIREAVKI
                     LISQGIIEIRRGKGTYVTEVPGIAQDPLGLDFVTTPNLRLDIIEARLLIEPGAARLAA
                     QKADSSDIAKIAASVKAMEEVVYRHKVEINAELDFHRSIAEATRNPVIMRIIPIIMES
                     IEKTYHDAPRTSEDHRHALEEHKVVYEAIAAHDPDRSFDAMQRHLEASYKRTLARQVP
                     "
     gene            complement(707..>1941)
                     /locus_tag="PK105_09280"
     CDS             complement(707..>1941)
                     /locus_tag="PK105_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOU61951.1"
                     /translation="SAAATVLDGYPLAKKGEAGKALGVALVASTLGGILSTLCLIFLA
                     PQLAKIALKFGPPEYFALMIFGLTIMASVSSASLTKGFLSGFFGLLLSMVGLNPTLGT
                     ERFTFGVTRLLTGFSMMPVLIGLFAVSEVFAQLELLSEGKIVQLSKKHKIGSIMPTKK
                     EMKGLWKVILGSSVLGTIIGIIPATGGSIASFMAYNEAKRFSKDPDSFGTGNLAGIAA
                     PESANNATTGGAMIPLLTLGIPGDVVTSVMLGALMIIGVQPGPLLFSQSPQIVTDLFV
                     GFMMAQFIMLALGYISIRASNLILRVPLGILYPTILCLCLLGAYSLGNSVYDVMLALI
                     FGIVGYFIKKYGFSAPSIILGLILGPIAEQELSRALIISHGDWTTLVRSPLAILFYVF
                     AIASIIYSFRSFKKGKTK"
BASE COUNT          579 a          471 c          465 g          426 t
ORIGIN      
        1 aggaagtcgc ttctcatatc aaggaactca tccgcgatga gaaactgaaa cctggcgaga
       61 agcttcccaa cgaactcgaa ctatgtggcc ttttttctgt ttcgcgcccc accataagag
      121 aagcggttaa aatcctcatc tcacagggga tcatcgagat acgccgagga aaaggcactt
      181 atgtaacaga ggtgccaggc attgcgcaag acccactagg tttggacttt gttaccaccc
      241 ccaatctccg tctcgatatt atcgaggcac gccttctaat agaaccaggt gcggcccgcc
      301 ttgccgccca gaaagccgac agcagtgata tcgccaagat agcggcaagc gtaaaagcca
      361 tggaggaggt cgtctatcgg cataaagtag agataaacgc cgagctcgac ttccatagga
      421 gcattgcgga agctacgcga aatcctgtaa taatgcgaat cattcctatt atcatggaat
      481 cgatcgagaa gacctaccac gacgcaccac ggactagcga agatcatcgc cacgctttgg
      541 aagaacataa agtagtctat gaggctatcg ctgcccatga tccagataga tctttcgacg
      601 ccatgcagcg tcatctcgaa gccagctaca agcgtacctt ggcgaggcaa gtaccttaga
      661 ggccttttag agccaaactc tcaatctaaa tatcgatttg ttgattctac ttggttttgc
      721 ctttcttgaa gcttctaaaa gagtagatta tcgaggcaat tgcgaagaca tagaacaaaa
      781 tcgcaagagg cgaacgcaca agggtagtcc aatccccatg ggatataatg agcgccctgc
      841 ttagttcttg ttctgcaata ggtccgagaa tgagccccaa tataatcgat ggtgcagaaa
      901 atccatattt cttaataaaa tagccgacaa ttccgaatat aagcgcaagc atgacatcgt
      961 agacgctgtt gcccagagaa taagctccca gaagacatag acatagaatc gtgggataga
     1021 ggatacccag aggtactctc aggatgagat tggaggcgcg aatgcttatg taacccaaag
     1081 ccagcatgat gaattgtgcc atcatgaatc cgacaaacag atcggtgaca atttgaggcg
     1141 actgcgagaa aagaaggggt cctggttgca ctccaatgat cataagagcg cccagcatga
     1201 cagaggttac gacatctcca ggaattccta aggtgagaag aggtatcatc gctccaccag
     1261 tagtcgcatt gttagccgat tctggcgctg ctatcccggc aagatttccc gtaccaaagg
     1321 aatcgggatc cttcgagaat cgttttgctt cgttgtaagc cataaaagag gcaatagaac
     1381 caccggtagc aggaattatc cctattatag tccctaggac cgaagaccct agaatcacct
     1441 tccagagacc cttcatttcc tttttagtcg gcatgatgga tccgattttg tgcttttttg
     1501 aaagctggac aatctttcct tcgcttaaaa gttccagctg ggcaaagact tcggataccg
     1561 caaagagacc aatgagcaca ggcatcatgg aaaagcccgt cagaagtcgt gttacgccaa
     1621 aagtaaagcg ttcggtaccc aaggtggggt tcaagccgac catcgaaagg agaagtccaa
     1681 aaaagcccga tagaaaaccc ttagtcaggg acgctgagga gacactagcc attattgtga
     1741 gaccgaaaat cataagcgca aaatattcag ggggtccgaa tttaagggca atctttgcaa
     1801 gctggggcgc aagaaagatt aggcagagcg tggataagat gccacccaac gtcgaagcaa
     1861 ccaaggcaac acccaaagcc tttcctgcct ctcccttctt ggcaagtgga tagccatcga
     1921 gcactgttgc tgccgcagat g
//
LOCUS       DAOOWY010000215         1934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_59281_length_1934_cov_6.846195, whole genome shotgun sequence.
ACCESSION   DAOOWY010000215 DAOOWY010000000
VERSION     DAOOWY010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59281_length_1934_cov_6.846195"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PK105_09285"
     CDS             complement(<1..252)
                     /locus_tag="PK105_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU61952.1"
                     /translation="MEGEKQRAPRNKTLTMNIVEKEKYSKRLLIPDGSPLFPSKIINK
                     TIYSDLFNIIDYLPKSFVDLLVIDPPYNLDKDFNGIRFSK"
     gene            399..>1934
                     /locus_tag="PK105_09290"
     CDS             399..>1934
                     /locus_tag="PK105_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU61953.1"
                     /translation="MLYFEKFNLAELPELGAAKYGLRPSLSMVVGSFYTYMDLERLSR
                     GVAQALALKGVKKGDRVALLAENSPHWVIAWFGIVRAGAVVVPILNDFMPKQIANIIQ
                     HSGAKIVFGSKKLQAKLADMSPKPDIWDVSESLEPSTDYVELPAVEADDLAMIIYTSG
                     TTGISKGVMLSHRNILSNALACKSIITLRRTDRLLSILPLAHTYEFTIGMVIPLLYGA
                     HIFYLDRPPSASALLPALKAVKPTIMLSVPLVIEKVYQSGIAPELNGMKLYKNKLMRP
                     LILRIAGMKLKKTFGGHLRFFGIGGAPLAAEVEEFLKKAHFPYAIGYGLTETAPLLAG
                     CAPKVTFLRSTGPALKGVEVRIADPKTITGEGEIQARGPNIFKGYWRDEVRTKEAFTE
                     DGWFRTGDLGSIDNKGRLFVRGRLKTMILGASGENIYPEEIESILNQMPEIEESLVVE
                     EEEGLTALVCLKSEVLDNIGARLQDHLDAVGDLSSRVGQAIAQAEKSVTGSVNQALTD
                     TEKA"
BASE COUNT          522 a          406 c          458 g          548 t
ORIGIN      
        1 tttggaaaat cttattccat tgaaatcttt atccaaatta tagggagggt caataacaag
       61 caaatcaaca aatgactttg gcagatagtc aataatattg aataggtcag aatagattgt
      121 cttatttatt attttacttg gaaataatgg gcttccatca ggaataagca gccttttact
      181 atatttttct ttttctacaa tattcattgt gagagtctta ttccttgggg ctcgctgttt
      241 ttctccttcc atatgactac ttcttcttat tataagaata aagcttacct tagagcaagg
      301 cttgtcacta tgtgattgaa taaaaagcga gccctctatc ccaatactct ttttcacagt
      361 aaactttgtt attcttgtat ttaatataaa gaggaaatat gctttatttt gaaaaattca
      421 atcttgcgga gctgcccgaa ttaggcgcag ccaaatatgg tttgaggcct tctctatcga
      481 tggtagtcgg ctcgttctat acctatatgg atttagaacg attgagcagg ggagttgcgc
      541 aagctctggc gttgaagggc gtaaagaagg gcgaccgcgt ggctctattg gccgaaaatt
      601 ctccccattg ggtaattgct tggtttggga tagtcagagc gggagccgtg gttgttccaa
      661 tcttgaacga tttcatgccg aagcagattg ccaatatcat tcaacattca ggcgcaaaga
      721 tcgtttttgg atccaagaaa ttgcaggcaa agctcgccga tatgtcaccc aagccagaca
      781 tctgggatgt ttccgagagt cttgagccat ccaccgatta tgtcgaattg ccggctgtcg
      841 aagcggatga tcttgcaatg atcatatata cctcaggcac aacaggtatt tcaaaaggcg
      901 ttatgctaag tcatcgcaat attctgtcca acgcgcttgc atgcaagagc ataataactc
      961 tacgaaggac agatcgcctt ctctcgattc ttccgcttgc tcacacctat gagtttacaa
     1021 ttggcatggt aattcctctc ttgtatggag ctcatatttt ctatcttgac aggcctcctt
     1081 cagcatcggc tctacttcca gcgttaaagg ccgtaaagcc tacaattatg ttgtctgtac
     1141 cgcttgtaat agagaaagtg taccagagtg gcattgctcc cgagctaaat gggatgaaat
     1201 tgtacaagaa caagctcatg cggcctttga tactccgcat tgcgggaatg aaattaaaga
     1261 agacttttgg tggtcatctg cgcttttttg gaattggcgg agcgcctctg gcggcggagg
     1321 tcgaagaatt cctcaagaaa gctcatttcc cttatgcgat aggctatgga cttactgaga
     1381 ccgctcctct tcttgcgggt tgcgctccta aagtgacttt tttgcgttcc acgggtccag
     1441 cgctcaaggg agtagaggtg cgcatagccg atcctaagac cattactgga gaaggcgaaa
     1501 tccaggcaag gggccccaat atcttcaaag gctactggag agatgaggtt cggacaaaag
     1561 aagctttcac tgaagatgga tggttccgca cgggagatct aggatcgatc gataataaag
     1621 gtcggctttt cgtacgcgga cgactcaaga caatgatctt aggcgcatcg ggcgagaata
     1681 tctatcccga agagatcgaa tcgatcctca atcagatgcc tgagatagag gaatcccttg
     1741 tggttgaaga ggaagaggga cttaccgcgc tagtatgcct taaaagcgag gtcctggata
     1801 atattggtgc gcgtttgcag gatcatctcg atgccgttgg agatctgagt tcgcgtgttg
     1861 gacaagctat cgctcaagct gaaaaatccg ttacaggcag cgtaaaccaa gccttgacag
     1921 atacggagaa agct
//
LOCUS       DAOOWY010000216         1922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_59817_length_1922_cov_11.554365, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000216 DAOOWY010000000
VERSION     DAOOWY010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59817_length_1922_cov_11.554365"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PK105_09295"
     CDS             <1..338
                     /locus_tag="PK105_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61954.1"
                     /translation="NYAAGLGVRLFENGKFEPSIYTELENPQKVFSSSDIAAKALNSL
                     HVGAQVRLLRFITVRGGINKGWYSMGAGIDLSFLELDAAIFTEELGPYPGDKGRSGLS
                     MQVGIRFGR"
     gene            560..1726
                     /locus_tag="PK105_09300"
     CDS             560..1726
                     /locus_tag="PK105_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61955.1"
                     /translation="MNLRLNTIAAIILFVVFSPITLIAQTNLTNLLPKNARSMALGGS
                     SLVFAEGCDALWGNPAGLSRKKSLTLFDSSTWAYIMPTPLNIQNLAAILNKEKSSEEI
                     TAILDDFVAENGIGGGEYFQFGWIDDGIGLGITSISDAVATGSDLANSVLDARSQINA
                     VIGMGWLTHLGPFEARLGVNVRGFYRIETSSDGWNFGPISEALISNADIYSVIQTDKL
                     MGGLGFAIDAGGTLAFGPFSVGLMVRDLADRFAMKESSIKEIADKYMVPMGGLDYYAI
                     SPIYTAGFSLALDQDSLLPVTLFVEADDPLSLFSLLPSNIQEIPSKMHFGLEIGILKF
                     STLRVGFNQGCFSAGVGIHLLFLDLNAAMFTEPLDIAGSKTKRSGLVLQGAIKF"
BASE COUNT          502 a          394 c          500 g          526 t
ORIGIN      
        1 ctaactatgc tgcagggctt ggtgtaaggc tattcgaaaa tggaaagttc gagccgagca
       61 tatatacaga gctggaaaac ccgcagaagg tcttttccag cagtgacatc gcggcaaagg
      121 cactcaactc gctacatgta ggtgcacaag tgcgactttt gcgcttcatt actgtgcgag
      181 gcgggataaa taagggatgg tatagcatgg gagccggcat agatctctcg tttttggagc
      241 tggatgctgc aatcttcacg gaagagctag gaccttaccc aggggataag ggaaggagtg
      301 gtctttctat gcaagttggg atcagatttg gtcgctgata atcgatatta ataggtgtta
      361 ttgttgattg agattattac ttgaaaaggt agtcaaaggg gcatagaagg ccggaggaga
      421 ctttaagaga agacttaaaa aggattggcc gccagcatct aaatgctggc ggcctttttt
      481 cttgatttat gtgtatttta tggcaatttc gttccgatac tttaagagaa gatatgattt
      541 catacaagag gaatgatcat tgaatctgcg cctaaatacc atagcagcca ttattctctt
      601 tgtagttttc agcccgatta ctctaattgc tcagacgaat ttgaccaatc ttcttcctaa
      661 gaatgcgcgc tctatggcac tgggaggttc atcgcttgta tttgccgagg gctgtgatgc
      721 tctatggggg aacccggctg gactatcgcg caaaaaatca ttgacgctct tcgattcgtc
      781 tacgtgggct tacataatgc caacgccgct caatattcag aatctggcag caatcctaaa
      841 caaagagaag agctcagaag agataacagc tattctggat gattttgtcg cagaaaatgg
      901 aataggaggc ggcgaatact ttcaatttgg ctggatcgac gatggcatag gtctgggaat
      961 tacctcgatt tcggatgcag tagcaacagg tagcgacctt gcgaattctg tcttagatgc
     1021 caggagccag ataaatgctg tgattgggat gggttggctc acacatctgg ggccttttga
     1081 ggcaaggctt ggggttaatg tgcgcggctt ctataggata gagacaagct cggatggatg
     1141 gaactttggg cctatttcgg aagccctaat atcgaacgcc gatatttatt ccgttatcca
     1201 aaccgataag ctgatggggg gattgggctt tgcaatagat gctggcggaa ctttagcctt
     1261 tgggcctttc tccgtgggtc tcatggtgag ggatttggca gacagattcg ccatgaagga
     1321 atccagcata aaagagattg ccgacaaata tatggttccc atgggagggc tcgactacta
     1381 tgcaatctcg ccaatctata ccgctgggtt ttccctagcc cttgatcagg attctctttt
     1441 gccagtgaca ctatttgtcg aggccgatga cccattatcg ttattctcgt tattgccgag
     1501 caatatccaa gaaataccct caaaaatgca ttttggcctt gagataggta tcttgaaatt
     1561 ctccactctg cgagttggat tcaatcaagg ttgcttctct gccggagttg gcatccatct
     1621 gcttttcctt gatttgaatg ccgcaatgtt taccgagcct cttgatattg caggcagcaa
     1681 aacaaaacgc tctggtcttg tgctgcaggg agcaatcaag ttctgactaa aaaagaattc
     1741 agcaatttgt ggggtagagt acaaatctgt gcaatagcga tcaagcaaat gcggaagctt
     1801 gacatttcat gcggttttcg ctagtgttac aacccgcgaa aggcgcacga ggtgcacaag
     1861 aagcttacgg gccgctagct cagttggaag agcaacgccc ttttaaggcg tgggtcccca
     1921 gt
//
LOCUS       DAOOWY010000217         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_59906_length_1920_cov_7.999464, whole genome shotgun sequence.
ACCESSION   DAOOWY010000217 DAOOWY010000000
VERSION     DAOOWY010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59906_length_1920_cov_7.999464"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..976)
                     /locus_tag="PK105_09305"
     CDS             complement(146..976)
                     /locus_tag="PK105_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61956.1"
                     /translation="MRVIEQFVQSKTGIQESCEDGYVITDCFAAVIDGVTNKNAVQLG
                     LDAPGKIAMELIKKGIGDLSPILSAHDAFKAINELIYDSYCNLGLVEAMKKNPSQRFS
                     ASCVIYNSVRRELWFLGDCMALANGTIYQFSKESDRLLSDLRSFLIYIQLSQGITEEQ
                     LLERDLCRQQIHSFLVSQTFLQNSSQNCPFAYYVLDGISEEAPRHAVVISVDEKTTEI
                     VLASDGYPTIYPTLEQTEAYLAHILEIDPLCYREFRSTKGLVKGNISFDDRAYVRLAI
                     "
     gene            1055..1801
                     /locus_tag="PK105_09310"
     CDS             1055..1801
                     /locus_tag="PK105_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOU61957.1"
                     /translation="MRICIFENALDLGSHSAMVVSMHIRRALQEKGECSIILATGTSQ
                     LTMLDALVKDHDIDWTKVEVFHLDEYVGIPETHKASFRKYLRERFADRVPGLRSFHYI
                     KADADDLSKEIARLSDLISSKNIDVACIGIGENGHLAFNDPPANLDTKEPYLIVRLDE
                     ACRRQQVGEGWFDDLTEVPMQAISMSIPQILKSECIICTVPDKRKAEAVRMAVTAPID
                     PIHPCASLRMHKNCYLMLDHSAASLICPLS"
BASE COUNT          551 a          400 c          422 g          547 t
ORIGIN      
        1 tgcagcggtt gcggatggca agatgggaga agctagcgca ttgtctacag tgctgctcgt
       61 tgtagtattc attattgtat acctgatgaa tacttttcag ggaaataaaa tggcggatag
      121 cttcaaaatg ggctaacccg atttactata ttgctagacg tacataagcc ctatcatcaa
      181 aagagatatt cccctttact aagcccttag tgctgcgaaa ttcacggtag caaagggggt
      241 cgatttcaag aatatgcgca agataggctt ccgtctgttc aagtgtagga taaatagtag
      301 gatatccatc gcttgctagg actatctctg tggtcttttc atcaacagaa ataactactg
      361 catgtcttgg tgcttcttcg cttatgccat cgagaacata ataggcaaaa gggcaatttt
      421 gcgatgagtt ctgaaggaaa gtctggctga ctagaaagct atgtatctgt tggcgacaca
      481 gatcgcgttc caaaagctgt tcttctgtta ttccctgaga gagctgtata tagataagaa
      541 aggagcgcaa atcactaaga agccgatctg attccttaga aaactggtat atggttccat
      601 tggctaaggc catgcaatcc cctagaaacc aaagctcacg ccgcactgaa ttgtagatta
      661 cacaagatgc agaaaaacgc tgggaaggat tcttcttcat agcttccact aaacccaagt
      721 tgcagtaact atcatagatc aattcattga tggccttaaa agcatcatgt gcagatagta
      781 ttggcgaaag atctccgatg cccttcttta tcagctccat ggcgattttc cctggcgcat
      841 ctaagcccaa ctgcacggca ttcttattgg ttactccatc tattactgcc gcgaaacaat
      901 cggttatgac atatccatcc tcgcaacttt cctgtattcc ggtttttgat tggacaaatt
      961 gttctattac tcgcatcttt agttccttat attccttcct tctgcattta tagtaaatta
     1021 caataacaga acaaaactca ggaggaaagt gctaatgaga atctgtatct ttgaaaatgc
     1081 tcttgatctg ggtagtcatt cagcgatggt tgtctccatg catattcgca gggctctgca
     1141 agaaaaggga gagtgttcta tcattctagc aactggcaca agccaattaa ccatgcttga
     1201 tgcccttgta aaggatcatg atatcgattg gaccaaggta gaggtttttc atctggatga
     1261 gtatgtaggc atacccgaga cacacaaggc aagtttccgc aagtatttgc gagagagatt
     1321 tgcggataga gtgcctggat tgcgatcttt ccactacatt aaggccgatg cagatgattt
     1381 gtcaaaagaa atagctagac tttctgattt aataagctca aagaacatcg atgtcgcgtg
     1441 cattggcatt ggagaaaatg ggcatcttgc atttaacgat cctccagcaa atctcgatac
     1501 gaaagagcct tatctgatag tccggctaga tgaagcatgc aggcggcaac aggttggaga
     1561 aggatggttc gatgatttaa cagaggtccc catgcaagct atttcgatga gcattcccca
     1621 gatactcaag agtgaatgca taatctgcac agttccggat aaaagaaagg ccgaagctgt
     1681 gcgtatggcg gtaaccgctc cgatcgatcc aattcatccc tgtgcatcgc tcagaatgca
     1741 taagaattgc tatttgatgc tagatcattc tgcagcttct cttatttgtc ccttgtcttg
     1801 agcatatcgt tatgatcacc aataacatga attcctatgg taccctaata gatctgcagg
     1861 tcaatggata tggaggaatc gattttagcg caaaagatct tactgtcgaa caggttgtct
//
LOCUS       DAOOWY010000218         1903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_60615_length_1903_cov_23.175325, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000218 DAOOWY010000000
VERSION     DAOOWY010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60615_length_1903_cov_23.175325"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PK105_09315"
     CDS             complement(<1..1273)
                     /locus_tag="PK105_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61958.1"
                     /translation="MKNQNANKAGLWFDIAPLIELTDRPSLTTTDNVSADDLAWYYSR
                     PSFITMGFQLHTGPLFAAMSIELQQDVGELFLGGSLTNLLVTHDWSEPCFSNNYPSVG
                     FIEGRDQGWFFSLGRRAINMGPGEYSLTLSSQNPWYDHFLGELSAPLDPGSLTYDFLA
                     VNVQNRSNNGKYLFMHRIQAQFPIFSIGISEYNLVTRSPLDFQDIGPFLVYHHLFASG
                     SNVMIQLDAQVMLLDNVRLYAEGLMDDFQLSTEGTSSNPNAFGFHAGLEWAIQPNSED
                     PYHRPRFFRKDYTTRIGASALEGALVASLEWYWASTYLYRRISSASNQAYFTRYFLQS
                     QYFGTRIIEPWFAYPLGPDRMMFSGGLRYEKGKVAASLRGSFFILGAESEVQTYAKPY
                     AKDWMGPQSPVTYNAQAKIALEYLASPSTLLW"
     gene            complement(1442..>1903)
                     /locus_tag="PK105_09320"
     CDS             complement(1442..>1903)
                     /locus_tag="PK105_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61959.1"
                     /translation="NYVVAIRMFSNSFGISYTEDFMGYQYGCDAIVYNGNIGYKQYGK
                     WYVSMNLFYMVHGTHDKWTLWTDVSDASLYVSTPSTQHNTGNNGDLGAETARNTASNT
                     IIVGLSGGYTVIENLNLYGEADYIFIENPGNISSNAPIYDIQLTVGVSYSL"
BASE COUNT          560 a          420 c          435 g          488 t
ORIGIN      
        1 accagaggag agttgaggga gaagcgaggt attctaaggc gatcttagct tgtgcgttat
       61 atgtgacagg gctttggggt cccatccaat ctttagcgta gggtttggca tatgtctgca
      121 cttcggattc tgcgccgagg ataaagaagc ttccgcgcag cgatgccgcg actttacctt
      181 tttcatacct gaggccaccg gagaacatca ttctgtctgg accgagagga tatgcaaacc
      241 agggttcaat aatgcgcgtg ccaaaatact gagattgaag aaaataccgc gtaaaatatg
      301 cttgatttga ggcacttgaa atgcgtcgat acaggtaagt cgaagcccaa taccattcta
      361 gagatgctac aagggctccc tcgagggctg atgctccaat tctagttgta taatcttttc
      421 tgaaaaatcg aggtctatgg taaggatctt ctgaatttgg ctggattgcc cactcgaggc
      481 cagcatggaa tccgaaggca ttggggttgg agctagttcc ctcagtgctc aattgaaaat
      541 catccatgag gccttcagca tatagccgga cattgtcgag gagcattacc tgtgcatcta
      601 gctgtatcat gacattcgag ccagaagcaa agaggtgatg ataaactaaa aatggtccaa
      661 tatcctgaaa atccaatggg cttcgcgtga cgaggttgta ttcggaaatt ccaatggaaa
      721 aaataggaaa ttgtgcctga attcgatgca taaagagata ttttccatta ttcgaacggt
      781 tctgaacatt gacagcaagg aaatcgtaag tcagagagcc cggatcaaga ggcgcagaaa
      841 gttctccaag aaaatggtca taccatggat tctggctcga cagtgttagg gagtactctc
      901 caggtcccat gttgattgct ctcctgccta gcgaaaagaa ccatccctga tctctgccct
      961 ctataaatcc tacgcttgga taattattgg agaaacaagg ctcagaccaa tcatgagtga
     1021 caagaagatt ggtcaaggaa ccaccaagga atagctcacc tacatcctgc tgcaactcga
     1081 tcgacatcgc agcaaagagc gggccagtgt ggagctggaa tcccatggta atgaaagaag
     1141 gcctcgaata gtaccaagca agatcatcag cactgacatt gtcagtggtg gtcaaagatg
     1201 gcctatcggt caactctatt agaggagcga tatcgaacca caatccagcc ttatttgcgt
     1261 tctggttctt caaagctgcc gactccttac tgcacacagg ggaaatgaag aaacacgcaa
     1321 aaaccaagct aaagaaaaaa ctaaagaaaa gagtagattg ttttgtaagc caccttttca
     1381 tttgcaagac tccaattaga cctttctagc atgattagac ccctctaagc ctgcagatag
     1441 atcacagcga ataagataca ccaacggtca actgaatatc gtatataggc gcattgctac
     1501 ttatgtttcc tggattctcg ataaaaatgt aatctgcctc cccatataag tttagattct
     1561 cgattacagt atatccgcca ctaagcccga caattatggt attgcttgct gtatttcgcg
     1621 cagtctccgc gcccaaatct ccattgttgc ctgtgttgtg ctgggtgctt ggtgtcgaga
     1681 catatagact cgcgtcggaa acatcagtcc aaagcgtcca cttgtcgtgg gttccatgta
     1741 ccatataaaa gagattcatt gagacatacc atttgccgta ctgcttgtat ccgatattgc
     1801 cattgtagac aattgcatcg catccgtatt ggtaacccat aaaatcttct gtataggaaa
     1861 ttccgaagct atttgaaaac atacggatgg caacaacata att
//
LOCUS       DAOOWY010000219         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_61068_length_1894_cov_9.701468, whole genome shotgun sequence.
ACCESSION   DAOOWY010000219 DAOOWY010000000
VERSION     DAOOWY010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61068_length_1894_cov_9.701468"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK105_09325"
     CDS             <1..233
                     /locus_tag="PK105_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61960.1"
                     /translation="DFVHYRDLIAHAYLDSLEEVAGSSRREAASPQALSMPNDILFLS
                     ILKKISYDGVITLPNYADCETLISYKEAWNKL"
     gene            complement(237..1364)
                     /locus_tag="PK105_09330"
     CDS             complement(237..1364)
                     /locus_tag="PK105_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU61961.1"
                     /translation="MKIIIIGNGIAANSAATVIREHDRESNITMLSDENQFFYARPRL
                     TEFISGKSAFEKILIHDEAWYKKNNIELLKGLSVSSIDTEHQVLYGSFGSLSYDELLI
                     ASGASPSFPPFYRPQLEKVYALRTKEQADHIIATAQHSKSAAIIGGGLLGIDTAFALV
                     ERGMDTTIIEFYGWLLPKQLDQDSAAILRDLLSQKRLKFLFGKQTASISNEKNGLTLA
                     FADGTSILADMAIISAGVRSNIAFLKGTKVATGRGITIDHQMRTNVPHIYAAGDCSEF
                     EGKMYGVWSAAKEQGEIAGLAMIGQDVDYQGSVIGARIKAAGIDVASIGDFSMTPSTT
                     VEVFHEKDIFKKYFREGNKLKGAILIGDTSDYFKLQKEIAK"
     gene            1552..>1894
                     /locus_tag="PK105_09335"
     CDS             1552..>1894
                     /locus_tag="PK105_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOU61962.1"
                     /translation="MNRAREKTFIRIAAIVMAFTLPMMIIAAEDLPILTGKVIEVQKY
                     GNLTMDLKPKDLYDVGFELGDILNVTVGDVSLKIPFCTSYSDVDTGSLVVRDDQKNNL
                     LVVAINMGNFAT"
BASE COUNT          501 a          405 c          425 g          563 t
ORIGIN      
        1 aagacttcgt ccattataga gacttgattg cccacgccta tctggattct cttgaagagg
       61 ttgctggatc gagcagaaga gaagcagcat ccccgcaagc tttgagcatg ccgaatgata
      121 ttctttttct atctattttg aaaaaaatat cttacgacgg ggttattacc ttgcccaatt
      181 atgctgattg tgaaacgctc atatcataca aagaggcatg gaacaagttg tagaccttac
      241 tttgcaattt ctttctgtaa tttgaaataa tctgatgtgt cgcctatcag gattgcgcct
      301 ttgagcttat tgccttcgcg gaagtatttt ttgaaaatgt ctttttcatg gaaaacttcc
      361 acagtggttg aaggcgtcat cgagaaatcg cctatggaag cgacatcaat gcccgctgct
      421 ttgattctgg cgccgatgac acttccttga taatcgacat cctggccaat catcgcaaga
      481 cctgcaattt ctccttgctc ttttgctgcc gaccatacac catacatctt gccttcaaac
      541 tcagaacagt cgccggcagc ataaatatgg ggaacatttg ttctcatttg atgatctatg
      601 gtgatgccgc gacctgtcgc taccttcgtg cctttcaaga atgcgatatt ggatctgacc
      661 ccagcggaga tgatcgccat gtcggccaaa atacttgtgc catcagcaaa agcaagggtc
      721 aatccgtttt tctcattcga gatggaagct gtctgcttcc caaacagaaa ttttagcctc
      781 ttttgagaga gcaaatctct tagaatggct gctgaatctt gatcgagttg cttcggcaga
      841 agccagccat aaaattcgat aattgttgtg tccatgccgc gttcgacaag cgcaaatgcg
      901 gtatcgattc ccagaaggcc tcctccgatg attgcggcac ttttgctatg ttgcgctgtg
      961 gcaatgatgt ggtctgcttg ctccttggtc cggagcgcgt agactttttc gagctgcggt
     1021 ctataaaaag gaggaaatga aggagatgcg cccgaggcta tgagcaactc gtcgtagctg
     1081 agggagccaa aagagccata aagtacttga tgctcagtgt cgatagagga aacgcttaga
     1141 cctttcaaga gctcaatatt gttcttttta taccatgctt catcatggat tagtattttt
     1201 tcaaaagccg attttccaga tataaattcc gtaagcctcg gtctcgcata gaaaaattga
     1261 ttctcatctg aaagcatggt aatgttgctt tctctatcgt gttctcgaat tactgttgcc
     1321 gcagaattcg ctgctatgcc attcccgatg atgattattt tcattgaatc cgatcctttt
     1381 ctatcaatat agagcaattg atcgaaatgg ccaagcagct ttcggaagaa ttgacattcg
     1441 atctgaacaa aagcttgtat gaggatttat cgggcttgag caggccaaaa tatgctaata
     1501 tactttgtat gagccaataa aagctaaatt tacttttgat ggaggtagcg tatgaacagg
     1561 gcaagagaga agacttttat tcgtatagcc gcgattgtca tggcatttac cttgcctatg
     1621 atgataatcg ctgcggaaga tttgcctatt ctgacgggga aggtcattga agtccagaaa
     1681 tatggcaacc ttactatgga tctcaaaccc aaagacctct acgatgttgg cttcgagctt
     1741 ggggatatct tgaatgtcac ggtaggagat gtctcgctca agattccttt ttgtacatct
     1801 tatagcgatg tcgatacggg cagccttgtg gtgcgcgatg atcagaagaa taacttgcta
     1861 gttgtcgcaa tcaatatggg caatttcgca acaa
//
LOCUS       DAOOWY010000220         1865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_62420_length_1865_cov_12.395580, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000220 DAOOWY010000000
VERSION     DAOOWY010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62420_length_1865_cov_12.395580"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..131)
                     /locus_tag="PK105_09340"
     tRNA            complement(58..131)
                     /locus_tag="PK105_09340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95..97),aa:Arg,seq:cct)
     gene            251..1492
                     /locus_tag="PK105_09345"
     CDS             251..1492
                     /locus_tag="PK105_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOU61963.1"
                     /translation="MLPKMKLWHKYALGIAMGAILYLVLPPSMIHGTPMISFLSELAL
                     KIGGYALILTLTVGIPISVFRLSEAHRFTKIFSQSLLLSVVSLVVAAGIGIAVVLVFK
                     PVPLPLVSQSGTVQTLDPLELILAIFPTSIFDVFSVSAKWFLPIGLFALFFGLALSHD
                     PVMSRPILPVLDVISRASYLINFFISEILGILLIPISLYLFLNLRNTGIPSEYRSVLL
                     FVLILTVFLIFVLFPLVYRILGGKSNPYILLYAMSGPLLTAAASGSIFISSATALRHV
                     SESLGIKRDTNAVVFPLVLVGGRVGSVIIVACSFIAMFLSYSRNNPTAGQIILLLALL
                     PLSVTVGASALRSDIIVMLSLVCVLFGQGFQNGASLLVPIALPLSICATILDTAWLMF
                     SLSIIAELNGQRSVKKARNFI"
     gene            1558..>1865
                     /locus_tag="PK105_09350"
     CDS             1558..>1865
                     /locus_tag="PK105_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU61964.1"
                     /translation="MDLKEILKPELISLDLKGRNKEEIIRELVDLASNSGKVLDKEET
                     IQSVLEREYRMSTGMKHGIAIPHGKTTAVHELVACVGITKEEVDFDALDRKGCRIFIM
                     T"
BASE COUNT          456 a          414 c          409 g          586 t
ORIGIN      
        1 taacaaaact caagtaacaa tgccaaatca caagcaatga tatgttgtaa ttccttatgg
       61 cccagggccg attcgaacgg ccgacctact gcttaggagg cagtcgctct atcctgctga
      121 gctactgggt cgcacattga aaagaacatt acttgaaaat aataggatct gtcaatgcaa
      181 agctaagcgc agtcggcatt agagaattcc agaaatctaa tcaatattga cctgtcaatc
      241 caatagagtt atgcttccca agatgaagct atggcataaa tacgcccttg gtatcgccat
      301 gggagcgatt ctctatctgg tcctgcctcc aagtatgatc catggcacgc ctatgatttc
      361 ttttctttca gagctggctt tgaagatcgg aggctatgcg ctcattctga ctctcacagt
      421 gggtattcct atttctgttt tcaggttgtc ggaagctcat cgcttcacaa agatcttttc
      481 tcagtctctg ctcctttcgg ttgtcagttt ggtagtagca gctggtatcg gcatagccgt
      541 agtcttggtt ttcaagcctg tgccattgcc attagtgtcc caaagtggaa ccgtacagac
      601 actcgatcca ttggaactta ttctagccat attccctacg agtatattcg acgtcttctc
      661 ggtttctgca aaatggttcc taccaattgg gctatttgct ctattctttg gtttagccct
      721 ctctcatgat cctgtgatgt cgaggccaat attgccggtg ctagatgtga tatctagagc
      781 atcttacctt ataaatttct ttatctcgga aatattggga attctactca ttcccatatc
      841 tttatatctc tttctaaatc ttagaaatac agggattcca tctgagtata gaagtgtcct
      901 tttatttgtg ctgattctga cggttttctt gattttcgtg cttttcccgc ttgtctatcg
      961 aattcttgga ggaaaatcca acccttatat acttctctat gcgatgtcgg ggccgcttct
     1021 cacagccgcg gcctcgggga gtatctttat ctcttcggcc acggctctgc gtcatgtatc
     1081 agagagctta ggaataaagc gcgatactaa tgcggttgta ttccccctcg ttcttgtcgg
     1141 tggtcgggta ggcagtgtaa ttattgttgc atgctcgttt atagcaatgt ttttgtctta
     1201 ttccaggaat aatcccactg ccggccagat aatcctattg ctcgcgcttc tgcctctttc
     1261 ggttactgtg ggagcttctg ctctacgtag cgacattatc gtaatgctct ctcttgtttg
     1321 tgtgcttttt ggacagggtt ttcaaaatgg agctagtctt ttggtaccga tcgctcttcc
     1381 gctctcaatc tgtgcgacaa ttcttgatac tgcttggttg atgttctcct tatcgattat
     1441 tgctgaactg aatggtcagc gcagcgtcaa gaaagcacgg aatttcatat gacctccgac
     1501 agacttgtct tgcggggtat acttgacggc atcaacagca caaattagga tagaataatg
     1561 gaccttaagg aaatattgaa gcctgaactc atttctttgg atctcaaagg caggaacaaa
     1621 gaagagatca tcagagaact tgtagatctt gcatcaaaca gtggaaaggt actggataaa
     1681 gaagaaacga ttcaaagtgt cctggaacgc gaatatcgta tgagcactgg catgaaacat
     1741 ggtattgcca ttcctcatgg taagacaaca gctgtacacg aattggttgc ctgtgtcggc
     1801 ataactaagg aagaagtgga ctttgacgcc ctcgatcgaa aggggtgtcg tattttcatc
     1861 atgac
//
LOCUS       DAOOWY010000221         1854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_62916_length_1854_cov_4.717621, whole genome shotgun sequence.
ACCESSION   DAOOWY010000221 DAOOWY010000000
VERSION     DAOOWY010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62916_length_1854_cov_4.717621"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1652)
                     /locus_tag="PK105_09355"
     CDS             complement(102..1652)
                     /locus_tag="PK105_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU61965.1"
                     /translation="MTTVEDKIYSLEAKGITKRFPGVLACDSIDLSIGENEILAIVGE
                     NGAGKTTLMNILMGLYQPDEGEIRINGRPVRFRTPNDAFAAGIGMVHQHFMLVPNMTV
                     AENVALGSRDFHRLLRLDLVGAKKRIIELSERFGLPVQPDSYVWQLSVGEQQRVELIK
                     TLCFGARFLILDEPTSALTPQETEELIVLLKRMAQEMSIIFISHKLAEVTALSDKVVI
                     LRHGRVVFSGTTSEQTTSGLAALMTGHEVILPKNESREECGDVCFELENVYAKSDRGY
                     MALDGVSFSIRQGEILGLAGVSGNGQKELAEAIAGLRKIESGRIRLFGKDLTNMRPRE
                     RISYGLGYIPEDRNSEGIVPSFSVKENLILKDFASSDFANGPFLKLKNGEQNAIELKE
                     KFDIRCPAVTTPAGALSGGNIQKVILARELARNPKVLIAVYPTRGLDMGAEEYIHKKL
                     LEMRSRGVAILLISEELEEIMNLSDRIAVMYKGKILRILTSKEASLRSIGLLMAGVTE
                     HMAMEAEQ"
BASE COUNT          458 a          506 c          402 g          488 t
ORIGIN      
        1 gctagctccg agaatgccaa gcacgatcgc ggcaatcgcc atggctacaa ctgcggcagc
       61 cagagaaatc agcacaatgt aaacaacgcg aagagatgat ttcactgttc cgcctccatt
      121 gccatatgct cggtgactcc tgccatcaac aagcctatac tgcgcaagga tgcctcttta
      181 gaggtaagaa ttcgaagtat tttcccttta tacattactg caattctatc cgataggttc
      241 attatttcct cgagctcttc ggaaatgaga agaatcgcaa caccccggct ccgcatttct
      301 aacagcttct tatggatgta ttcctcggct cccatatcga gccctcttgt gggatatacc
      361 gctatgagga cttttggatt cctggccaat tctctggcaa ggatcacttt ttgaatatta
      421 cccccggata aagcccccgc tggagtggtc acggccggac atcgaatatc gaatttctcc
      481 tttagctcga tggcattttg ctctccgttc tttagcttca gaaatggacc attagcaaaa
      541 tcggacgaag caaaatcctt gagaatcaga ttttccttta cggaaaatga tgggactatg
      601 ccctccgagt tcctatcctc cggaatataa ccaaggccat agctaatacg ttctcgcggc
      661 cgcatattgg tcaaatcttt cccaaaaagc ctgatacgac ccgattcgat ttttctgagg
      721 ccggctatcg cctccgcgag ctccttctgg ccattaccag acacaccagc aagcccaagt
      781 atttcaccct gccgaatgga aaaactcact ccatcgagtg ccatatagcc ccgatcggac
      841 ttggcataga cattctccaa ctcgaaacat acatctccac attcttcgcg gctctcgttc
      901 ttgggtagaa tcacctcatg tcccgtcata agcgccgcaa ggcctgatgt tgtctgctcc
      961 gatgttgttc ccgaaaatac gactctaccg tggcgaagaa taaccacctt atccgaaaga
     1021 gcagtaacct ccgcaagctt atggctaatg aaaattatcg acatctcctg ggccattcgt
     1081 ttgagaagaa cgataagctc ctctgtttcc tggggcgtaa gtgcgcttgt gggttcatct
     1141 agaatgagaa atcgggctcc aaaacaaaga gtcttgataa gttctactcg ctgttgttcg
     1201 ccgaccgaaa gctgccagac ataggaatcc ggctgaacag gcaggccaaa gcgctcggat
     1261 agctctatga tgcgcttctt ggctccaacg aggtcgagcc ttaagagtct atggaaatct
     1321 ctggagccaa gcgccacgtt ttccgccaca gtcatgttcg gcaccagcat aaaatgctgg
     1381 tgcaccatgc cgatcccggc cgcaaaggca tcattaggtg tgcgaaagcg cacgggtctc
     1441 ccatttatgc gaatctcgcc ttcatccggc tgatacagcc ccattagaat attcatgaga
     1501 gtagtcttgc ctgcgccatt ctctccgact atggcaagta tctcgttttc tccgatgctg
     1561 agatcgatgg aatcgcaggc aagaactccg gggaaacgct tggtaatacc ttttgcttct
     1621 agagaataga tcttgtcttc taccgtggtc attcacaacc cctggaataa gctttctcta
     1681 cggcttgcgc atgacaatcc aaagcgaccg ttcgaactat tttacgtctt tccacggaat
     1741 ggtcaatttt ccactggtta tgtcggcctt agctttctct gcggcttttc tgacttctgc
     1801 gctaagaatt ggccctactt tgtcgttgaa ctgatagaca aagccaccat tggt
//
LOCUS       DAOOWY010000222         1842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_63511_length_1842_cov_18.293229, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000222 DAOOWY010000000
VERSION     DAOOWY010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63511_length_1842_cov_18.293229"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PK105_09360"
     CDS             <1..470
                     /locus_tag="PK105_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61966.1"
                     /translation="EMIQNIVTTKMDASYIIQAASAERAVSVEKPTFDARVIEQQNGA
                     LYALNNFARPTYEPWKGKIVELPDNAYDDRQKCLDALFASPEWQKGVNLDMIKSMMDR
                     TIEKGGPVVEGALFGTVLQVIAIPEDMRLLFRSYGYSDWADIDLNTLFQKNKY"
     gene            534..>1842
                     /locus_tag="PK105_09365"
     CDS             534..>1842
                     /locus_tag="PK105_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU61967.1"
                     /translation="MKRLSFIFMAILCAALSPMPLLAQSGGSGNLDSNLEAAIAELSF
                     PDEGDYSELGWGASFFAMHDFLSRTYAFGDWKQIPWDSMASYFGQKIMAAQAEKDRNS
                     YRIAMVEYLKCIPDGHVKITGPLDDLKERFIGGSYGLGLGELDDGSIVVAAVKPEGPA
                     ALAGICPGAHIAAWNGVPIQDALVAADTRWFKNAATKDDLALERLQALTRAPVGTQAV
                     IEFFAATAEEQPQERDATAEESPKTRIATLTARSDNFADLALFDLAPIPTMEELRKNV
                     EWRILDENVGYLRIYHVIHFDDYAIYPTEVTDEVVNALDAFCAAGVNSLILDLRGNRG
                     GSDQVAADISGHFAEEPSLFERTAWYNASSGHFDYAHSDLFAQTFELGDGPLWVEPCQ
                     PHFSGKIAVLVNPATISSGEGLAMAIGRLPKATVMGFYGTHGSF"
BASE COUNT          462 a          400 c          498 g          482 t
ORIGIN      
        1 acgaaatgat ccagaacatt gtcacgacaa aaatggatgc gtcatacatt atccaagctg
       61 caagcgctga acgcgctgtc tctgtggaaa agcctacctt cgatgctcgc gttatcgagc
      121 agcaaaatgg tgcattgtat gctctaaata acttcgctcg ccctacctac gagccctgga
      181 aggggaaaat cgtcgaactt cctgacaatg cgtacgatga tcggcaaaag tgtctggacg
      241 cactctttgc gtcgccggaa tggcaaaagg gcgtcaattt agatatgatc aaatcaatga
      301 tggacagaac catagaaaaa ggtgggcctg tagtagaagg tgcgctcttt ggtaccgtgc
      361 ttcaggttat cgcaattccg gaagacatgc gattgctctt tcggagttat ggatactcag
      421 attgggctga tatcgatctg aatacgctct ttcagaaaaa taagtattga ccatattagt
      481 taactcgtaa aagtgattaa gattagacaa ttttgcaata taagaggaat agaatgaagc
      541 gactctcatt catctttatg gctattttgt gtgccgcgct aagcccaatg ccgctattag
      601 cccagagtgg aggatcggga aatctggatt cgaatctgga ggctgcaatt gccgaactct
      661 cttttcccga tgagggagat tatagcgagt taggttgggg agcaagcttt tttgctatgc
      721 atgatttttt atctcgaacg tatgcttttg gcgattggaa acaaattcca tgggactcaa
      781 tggcctccta tttcgggcaa aaaattatgg cggcgcaggc tgaaaaggat cggaattcgt
      841 atcgcattgc gatggtcgaa tacctgaagt gcattcccga cggccatgtg aagattacag
      901 gcccgctcga cgatttgaag gagcgcttta ttggtggcag ctatggactt gggcttggtg
      961 agctcgatga tgggtctata gtggttgctg cagtcaagcc ggagggccct gcagctctgg
     1021 ctgggatatg tccaggggcg catattgctg catggaatgg cgtaccgatt caggatgctc
     1081 tggttgcggc ggatacgcgt tggtttaaga atgcggcaac aaaggatgac ctcgctctcg
     1141 agcgattaca agcgcttacc cgcgcgcctg ttggtacgca ggcggttatt gaattctttg
     1201 cagcgacagc tgaggagcag ccacaagaaa gagacgccac ggcagaagaa tcgccaaaaa
     1261 ccaggatcgc tacgttgact gcgcgcagcg acaattttgc cgatcttgca cttttcgacc
     1321 tagcgccaat ccctaccatg gaagagcttc gcaagaatgt ggagtggcga atcctggatg
     1381 aaaatgttgg ttacttgcga atttaccacg tgatccactt tgacgactat gcgatatatc
     1441 caacagaagt gaccgatgaa gtagtgaacg ctttagatgc attctgtgca gcaggggtga
     1501 attcgctcat tttagatttg cgcggtaatc gtggcggttc cgaccaagtt gcagcagata
     1561 tatctggcca ttttgccgaa gagccttcgc ttttcgaacg aactgcttgg tataatgcga
     1621 gttcggggca tttcgattat gcgcattccg atctttttgc tcagacattc gagctaggtg
     1681 atgggccttt atgggtggag ccttgccaac ctcatttttc gggcaagatc gcggtgctgg
     1741 ttaacccagc gaccataagt tcaggcgaag gtcttgctat ggcaatcggt cgccttccta
     1801 aagcgacagt gatggggttc tatggtacac acggttcttt cg
//
LOCUS       DAOOWY010000223         1817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_64726_length_1817_cov_9.635641, whole genome shotgun sequence.
ACCESSION   DAOOWY010000223 DAOOWY010000000
VERSION     DAOOWY010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64726_length_1817_cov_9.635641"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="PK105_09370"
     CDS             complement(<1..1597)
                     /locus_tag="PK105_09370"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU61968.1"
                     /translation="MINGSSLLSIEHVSKDFYGNIVLHDVSFDLHKGEILGLVGENGA
                     GKTTLMHILFSMPVIAETGGFQGNIKIEGTEVHFASPFDALDAGLGMVHQEFSLIPGF
                     TTAENIVLNREPMHPTILNDVFGDRMSVLLRDKMRERAEKAINKLGVNLDPDMLVSEM
                     PVGHKQFTEIAREIDREQTRILILDEPTAVLTESEAEVLLQTLRRLASEGIGIIFISH
                     RLQEVLSIADRIIVLRDGHLVKDTPNQNINLRDVASWMVGRSIAGEEDKYQLRSFSNI
                     ALSVRNLWVDMPGETVRDVSFDVHKGEIFGIGGLAGQGKLGIPNGIMGLFPAGGEVIL
                     DGKPVPLNNPAMALAMGMGFVSEDRRGVGLLLDESLEWNIAFTAIQIHNQCLKKVFAG
                     LFTQRDEKAMEELCQEYIKLLDIKCTSTKQKAKELSGGNQQKICLAKAFAIKPQLLFV
                     SEPTRGIDVGAKKLVLDALNHYNRDFETTIVMVSSELIELRSICDRIAIVDEGRIAGI
                     LPPTADLSEFGLLMSGAAMEEAVN"
BASE COUNT          473 a          467 c          385 g          492 t
ORIGIN      
        1 cattgacggc ttcctccata gctgcgcccg acataagcag gccaaattca gagagatctg
       61 ccgtcggcgg caggatcccc gcaatccttc cttcatcgac aatagcaatg cgatcgcaaa
      121 tcgaacgcaa ttcgatgagc tcgctagaga ccataactat ggtggtctca aaatcgcgat
      181 tatagtgatt taatgcgtct aagactaatt ttttagcgcc gacatctatt cctcgtgtcg
      241 gctccgatac aaataagagc tgaggcttga ttgcaaaagc ctttgccagg cagatcttct
      301 gctgattgcc tccggaaagt tccttagctt tctgttttgt actggtgcat ttaatgtcca
      361 agagctttat gtattcttga catagctctt ccatagcctt ttcgtcgcgc tgtgtaaaga
      421 gcccggcaaa tactttctta aggcattgat tgtgtatctg tatagcagta aaagctatgt
      481 tccattcgag gctttcatcg agcaataggc caacgccgcg cctgtcttcc gaaacaaaac
      541 ccatgcccat ggcaagcgcc atagcagggt tgttgagtgg tactggcttt ccgtcgagta
      601 tgacttctcc tccggcgggg aataacccca tgattccatt ggggattccg agttttccct
      661 gaccagccag gcctccgatt ccaaaaatct cccccttatg gacatcgaag gaaacatccc
      721 ggacagtctc ccccggcata tcaacccaga ggtttcgtac actaagagct atattggaaa
      781 agctcctgag ttggtatttg tcttcctctc ctgcgatgct gcgacctacc atccaggatg
      841 caacgtctcg taaattgata ttctgatttg gcgtatcttt tacaagatga ccatctcgca
      901 atactataat acgatccgca atagagagaa cttcttgcag tctgtgggaa ataaagataa
      961 tcccgatccc ttccgaagca aggcgcctga gtgtctgcaa cagtacttct gcctccgatt
     1021 cggttagcac cgccgtaggt tcatccagaa tgagtatgcg agtctgttct cgatcgattt
     1081 ctctcgctat ctccgtaaac tgcttgtgac ctaccggcat ctccgacaca agcatatctg
     1141 gatcgagatt gacaccaagt ttatttattg ctttttcggc acgctcacgc attttatcgc
     1201 gcaatagaac gctcatgcgg tcgccaaata catcatttag aatagtcggg tgcatcggct
     1261 cgcggttcag cactatgttc tcagctgtcg taaaaccagg aataaggctg aattcctgat
     1321 gaaccatacc caacccagca tccaaggcat cgaatggact ggcaaaatga acctccgtcc
     1381 cttcgatctt tatgttgccc tgaaaaccgc cagtctcggc tatgaccggc atgctaaaaa
     1441 gaatgtgcat tagagttgtc ttgccagcgc cattttctcc aacaagacca agaatttctc
     1501 ctttatgcag gtcaaaactc acatcatgca gtacgatatt cccatagaaa tctttcgata
     1561 catgctcgat ggatagaaga gagcttccat ttatcataat aattccatta gaaatgggtt
     1621 tataagaaat ggcccgcatt ccgacaacgc ctgaatacgg gcctaaataa gagcttgttc
     1681 ggcgtctcaa gctaaaaatc agaaccacgc gcggaaagaa ttcggcgcat ggctcttctc
     1741 aactatttat gcatctggat attgtaatac ttatccggca ctttgacctg agtggtaggc
     1801 aagaagcctt tgccaaa
//
LOCUS       DAOOWY010000224         1808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_65147_length_1808_cov_26.812892, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000224 DAOOWY010000000
VERSION     DAOOWY010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65147_length_1808_cov_26.812892"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..281
                     /locus_tag="PK105_09375"
     CDS             45..281
                     /locus_tag="PK105_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61969.1"
                     /translation="MCEKNIIAVALAACICISALLSTAGACSADTVKFGLGASVETYW
                     HSNTTDFVDESDWVVGFEDGTCSITSSDLPSPKG"
     gene            441..758
                     /locus_tag="PK105_09380"
     CDS             441..758
                     /locus_tag="PK105_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61970.1"
                     /translation="MNLCFLENPYQVRPGLNHIIAIRMFSNTFGTSYTEDFMGYQYGC
                     DAIVYNGNIGYKQYGKWYVSMNLFYMVHGTHDKWTLWTDVSDESLNVSTPSTQHNTGK
                     SGE"
     gene            981..1286
                     /locus_tag="PK105_09385"
     CDS             981..1286
                     /locus_tag="PK105_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61971.1"
                     /translation="MMFYGGLRYEKGKVAASLRGSILILGAESEVQTYAIPYAKDWMG
                     PQSPVTYNAQAKIALEYLASPSTLLWSCFSIEGAIENTAILNFSLGLVWRLGLGSLE"
     gene            complement(1310..1768)
                     /locus_tag="PK105_09390"
     CDS             complement(1310..1768)
                     /locus_tag="PK105_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61972.1"
                     /translation="MKLRKSNYLIDSVLLLVLLLSACSLFPNLSFEDIKGEWELDSEI
                     VFNTYHLSSIHLSILEDDESGKKWWDLGWESDDRIDWFSSSDSEDALSEFEGNVYKGH
                     YVYWQEGEQVSHNITVTFTLSNDGRLTAEFVGDGPLDGLILEQLQKIETE"
BASE COUNT          494 a          422 c          376 g          516 t
ORIGIN      
        1 ctgcaaaata gctggtcttt caatcttatc tttcaaagag gaatttgtgt gaaaagaaca
       61 tcatcgccgt tgcactagca gcatgtatct gtatctctgc ccttctttcc accgctgggg
      121 catgcagtgc cgatactgtc aagtttgggt taggcgctag cgtcgaaacc tactggcata
      181 gcaatactac tgattttgtt gacgaatccg actgggtagt cggttttgag gatggaacat
      241 gctcaataac aagctcggat ttgccctcac cgaagggata atgtatcaat aggctgacaa
      301 tacccttgat ttgcgtatac tgaatcaagc cgcgatctac cacgattact acattcgcgg
      361 caattcgaat tccatcctct ctagagagct caattattcg cctatatcct atgttaatct
      421 ctatggacag atggtggtag atgaatttgt gcttcctgga gaacccctac caggtgagac
      481 cagggctcaa tcatattatt gctatccgta tgttttcaaa caccttcgga acttcctata
      541 cagaagattt tatgggttac caatacggat gcgatgcaat tgtctacaat ggcaatatcg
      601 gatacaagca gtacggcaaa tggtatgtct caatgaatct cttttatatg gtacatggaa
      661 cccacgacaa gtggacgctt tggactgatg tttccgacga gagccttaat gtctcgacac
      721 caagcaccca gcacaacaca ggcaaaagtg gagaatagac ctcgattttt cagaaaagat
      781 tatacaacta gaattggagc atcagccctc gagggagccc ttgtagcatc tctagaatgg
      841 tattgggctt cgacttacct gtatcgacgc atttcaagtg cctcaaatca agcatatttt
      901 acacggtatt ttcttcaatc tcagtatttt ggcacgcgca ttattgaacc ctgtttgcat
      961 atcctctcgg tccagacaga atgatgttct atggtggcct caggtatgaa aaaggtaaag
     1021 tcgcggcatc gctgcgcgga agcatattga tcctcggcgc agaatcagaa gtgcagacat
     1081 atgccatacc ctacgccaaa gattggatgg gaccccaaag ccccgtcaca tataatgcac
     1141 aagctaagat cgccttagaa tacctcgctt ctccctcaac tctcctctgg tcttgttttt
     1201 cgattgaagg cgccatagaa aacacagcta tcttgaattt ctccctagga ctcgtatggc
     1261 ggcttggtct tgggtcgctc gagtagaaaa atacccttat cctgtgtggc tattcggttt
     1321 cgattttctg caattgctcc agaataagac catcaagtgg cccatcgcca acgaattcgg
     1381 cagtcaaacg gccgtcattt gagagagtga aggttacagt aatattatgt gaaacctgct
     1441 cgccttcttg ccagtataca tagtggcctt tatatacatt tccttcgaat tcactcaagg
     1501 cgtcttctga atcagagctg ctaaaccagt cgatccggtc atcgctttcc catccgaggt
     1561 cccaccattt tttgccagat tcatcatctt caagtatgct tagatggatc gaagaaagat
     1621 ggtaagtatt gaagactatt tcggaatcta gttcccactc gcctttgata tcttcaaagc
     1681 tcagattggg aaatagtgaa caagctgata acaacagtac aaggagaagc acactgtcga
     1741 tcaaataatt agattttcta agtttcataa cgacctccca tttccaatta gatttcgatt
     1801 tctatttt
//
LOCUS       DAOOWY010000225         1808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_65148_length_1808_cov_10.843126, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000225 DAOOWY010000000
VERSION     DAOOWY010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65148_length_1808_cov_10.843126"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..>1808
                     /gene="nuoF"
                     /locus_tag="PK105_09395"
     CDS             188..>1808
                     /gene="nuoF"
                     /locus_tag="PK105_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOU61973.1"
                     /translation="MAYKNFILVCGGTGCESSRSDEIYRNLIKEAESLGVSRDVQVVK
                     TGCFGFCEKGPIVKVLPSESFYVQVKPEDAHDIIAEQVIKGREVTRLLYRKDKKTETT
                     KLEEIEFYQKQFRIVLRNCGVINPENIDEYIARDGYKALERVLFEMTPEEVIAELKAS
                     GLRGRGGAGFPTGMKWEAARKAPGDIKYIVCNADEGDPGAYMDRSTIEGDPHSILEAM
                     VIAGKTVEANFGYIYIRAEYPLAIERLKIAIAQAKEYGLLGKDILGSGFDFDIEIRLG
                     AGAFVCGEETALLQSIEGKRGMPRPKPPFPAMKGLWGKPTVINNVETLANVPVVLTRG
                     ANWFSSIGTDKSKGTKVFALTGKINNSGLIEVPMGTTLREIIFDIGGGIRNGKKFKGV
                     QTGGPSGGIIVEKDLDKPISYETLTALGSMMGSGGMIVMDEDDCVVDVSKFYMTFCVD
                     ESCGKCAPCRIGTKQMQMLLDKITRGQGEEADLAKLEQIGKAMTKASLCLLGGSAANP
                     TLSTIRHFRDEYLEHIHDKKCRAGKCKDLVVY"
BASE COUNT          524 a          392 c          479 g          413 t
ORIGIN      
        1 tatgctatgt agaacccacg gtcgaagtga ttatgcccga tatgccccct gtaatatatg
       61 gcaagatgac gagcgatgtg gcggaagaac tcgtggttaa gcacctgatc gaacacagtc
      121 tgctcgataa tcatatattc gacaagccag ccatcgacat tgcaaagcac tgagacggga
      181 ggatggtatg gcatacaaga acttcatttt agtctgtggt ggaacgggtt gtgaatcttc
      241 tcggagcgac gagatatacc gaaatctcat caaggaagcc gagtctctgg gtgtgagccg
      301 tgatgtgcag gttgtcaaaa caggatgctt tggattctgt gagaaaggcc caatcgtaaa
      361 agtgctaccc agcgaatcat tctatgttca agtcaaaccg gaagatgctc atgacataat
      421 tgcagagcag gttataaaag gccgtgaagt cactcgtctt ctctatcgaa aggacaagaa
      481 gaccgaaaca accaaactag aagagatcga attctaccag aagcaattta gaatagtcct
      541 tagaaactgc ggggtcataa atccagaaaa tatcgatgag tatattgccc gagatggtta
      601 taaggcactc gagagagtcc tttttgaaat gacgccagaa gaggtaatcg ctgagcttaa
      661 agcatctggg cttcgcggaa gaggtggagc agggttccca acgggtatga aatgggaagc
      721 tgctcgcaag gctcccggtg atatcaaata tatcgtctgc aatgcggacg aaggagatcc
      781 cggcgcctat atggatcgtt ccacgatcga aggcgatcct cactctattc tcgaggctat
      841 ggtgattgcg gggaaaacag tagaagcgaa cttcggatac atctacattc gagcagaata
      901 cccgcttgcc attgagcgac ttaaaattgc tatagcccag gcaaaggaat atgggcttct
      961 gggaaaggat attttgggct ccggattcga tttcgatata gaaatccgtc tgggtgcagg
     1021 tgcttttgtc tgtggtgagg agactgcgct tcttcagtct atcgaaggaa aacgcggaat
     1081 gcccaggcca aagcctccgt tccctgctat gaaaggactt tggggcaaac ccacggttat
     1141 caacaatgtc gagaccctgg ctaatgtgcc ggtggttctc acgcgcggtg ctaattggtt
     1201 ctcttccata ggaaccgaca agtccaaagg cacaaaggta tttgctctaa cgggcaagat
     1261 aaacaactct ggactcattg aagtacctat gggaacaacc ttgcgtgaga taatcttcga
     1321 cataggcgga ggcattagaa acggcaagaa gttcaagggc gttcaaacag gaggaccctc
     1381 tggcggtatc attgtggaaa aagacctcga taagccaatt tcctacgaaa ccctgaccgc
     1441 tcttggttcg atgatgggct caggcggtat gattgtcatg gatgaagacg actgcgttgt
     1501 cgatgtatcc aaattctata tgacattttg cgtcgacgaa tcctgcggaa aatgcgctcc
     1561 ctgtcgtata gggacaaagc agatgcagat gctgttggac aaaataacca gggggcaagg
     1621 cgaagaggcc gacctagcca agctcgaaca gataggtaaa gccatgacaa aggcatcact
     1681 atgcttgctg ggaggttcgg cagctaatcc gacgctatcg acgataagac atttccgcga
     1741 tgaatatctt gaacatatcc acgataaaaa atgccgcgca ggcaaatgca aggaccttgt
     1801 ggtctacg
//
LOCUS       DAOOWY010000226         1784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_66389_length_1784_cov_9.134760, whole genome shotgun sequence.
ACCESSION   DAOOWY010000226 DAOOWY010000000
VERSION     DAOOWY010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66389_length_1784_cov_9.134760"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PK105_09400"
     CDS             complement(<1..988)
                     /locus_tag="PK105_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU61974.1"
                     /translation="MSTELHESKPSIISNFLFSTLSTFLTILIPLLTYPYVARILGPE
                     NLGRTGIASSFTNYFIAAAQIGLPVYGVRLVAQRRSDKKALERAVSELVQLSIASSFL
                     AILAFSIAVMAIPKYRSEWNLFLVFGLTIMTSAMNMDWLFQGMERFRYIGVRNAILQV
                     LFVVSLFLFVRKASDYIVYAFLFFAVGFATMIINYAVARRNIKLGFKDLSPLKHIGSI
                     SVITLFSFLVTAYTNLDFLFLSLFSRIEEAGFYSISIRIARIVIAFMATLSTVLMPRL
                     STLAETDKEESTRLLQNSFSAIALFVIPAAFGLVAIADDLIKVFAGAQFTPAV"
     gene            complement(985..>1784)
                     /locus_tag="PK105_09405"
     CDS             complement(985..>1784)
                     /locus_tag="PK105_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61975.1"
                     /translation="RIFSLIILIIVAAGIVYADSKPSLKDVLPGLTNDEMRNLLASNL
                     LIEDYAKDGSSRFLPSDQTAMTLGESLADRKDGNKYETAYLINDIRFSGNDKLMFFNK
                     LAQINTLSGIMYYSETRGKDTILFDDVYIVDAPGSKTPQQPRFFSQIPERLSLFVHIK
                     DVNFGSIWFSILLFEKEDVFFFLIENVQPMGIGPIRAFDKGDFRIAFSFKEIQEGLLV
                     SSVSYFNPRRLASTFVDVFSAAEKRIRAISSWIAAQAIEAQNTPGHR"
BASE COUNT          588 a          376 c          364 g          456 t
ORIGIN      
        1 ttactgcagg tgtaaattga gctccggcaa atacttttat caaatcatcg gcaattgcta
       61 caagtccgaa agctgccgga attacaaata atgctattgc cgagaaagaa ttctgcaaaa
      121 gccttgtgct ctcttcttta tccgtttctg caagcgtcga aaggcggggc atgagcaccg
      181 tgctaagcgt tgccataaat gcaatgacaa ttctggcaat ccgaatagag atgctataga
      241 atcctgcctc ctcaatccgt gaaaacaagc tcaaaaacaa gaaatctagg tttgtatagg
      301 ctgttacaag aaaggaaaaa agcgtaatca ctgaaattga accaatatgc ttaagagggg
      361 aaaggtcttt gaatccaagc ttgatgtttc tacgagcgac agcataattt attatcattg
      421 tagcaaatcc aacagcgaag aacaaaaacg cataaacgat atagtccgaa gccttgcgta
      481 caaagagaaa gagcgaaacc acgaatagaa cttggagaat tgcattgcga acgccgatat
      541 acctgaagcg ttccatacct tgaaatagcc aatccatatt catggcggaa gtcattatag
      601 tcaatccgaa tactaaaaaa agattccatt cgcttctata ttttggaatg gccattacag
      661 cgatagaaaa agccaatata gccaaaaacg aagaagctat cgaaagctga acaagctccg
      721 aaacagctct ctcgagtgct tttttgtcgg atctacgctg agccacgagc ctcacgccgt
      781 agacaggaag cccaatttgc gctgctgcaa tgaaataatt tgtaaatgag gaagcaattc
      841 ctgtgcggcc gagattttcg ggcccaagaa ttctcgctac atagggatag gtgagaaggg
      901 gaatgagtat ggtcaagaaa gtactaagag tcgaaaaaag aaaattgctt attatcgaag
      961 gcttagattc atgcaactca gtactcatcg atgccccgga gtattctgag cctcgatagc
     1021 ttgtgctgct atccaactgc taatagccct aattctcttt tctgctgcgg agaaaacatc
     1081 cacaaaagtg gaagccaatc gtcttggatt gaaataactc acactgctaa caagaagacc
     1141 ttcctgaatc tccttgaaag aaaacgcgat tcgaaaatct cctttatcaa aggcgcgtat
     1201 agggcctatt cccatgggct gcacattttc gatcaggaaa aagaaaacat cttctttctc
     1261 gaacagcaaa atggaaaacc agatcgatcc aaaattgaca tcttttatat gtacaaaaag
     1321 actcagccgc tcaggaattt gagaaaagaa tcttggctgc tggggagttt tcgatcccgg
     1381 agcatcgacg atatagacat catcgaataa gatagtgtcc ttgcctcttg tttcagaata
     1441 atacataatt cctgatagag tattgatttg tgcgagctta ttaaaaaaca tgagcttatc
     1501 attaccagaa aaacggatat cattgattag atatgcggtc tcatatttat ttccatcttt
     1561 tcgatctgct agactttccc ccagggtcat ggctgtctga tcggagggca aaaaccgaga
     1621 ggaaccatcc ttcgcataat cttctataag aagattactt gccagaagat tccgcatctc
     1681 atcgtttgta agcccgggca aaacatcctt tagcgaaggt ttgctatctg catacactat
     1741 cccagctgca acaataataa gaattataag ggaaaatatt ctga
//
LOCUS       DAOOWY010000227         1782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_66498_length_1782_cov_15.338159, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000227 DAOOWY010000000
VERSION     DAOOWY010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66498_length_1782_cov_15.338159"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1316
                     /locus_tag="PK105_09410"
     CDS             270..1316
                     /locus_tag="PK105_09410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU61976.1"
                     /translation="MPEGLRRIKEREKRWYGRLAEGLASSFIALQVALRRLLAWGRTR
                     VVFAMIPQSERPSRKFEIRRFSLLAIVLTIVICIGAAIISIGAYSTKSVKVISLEAQL
                     SDAYRSIDRLRESSENLLSETAKFEEKLGQVIEIAQSKGKPAVQQSTSEEILKVAGLD
                     DPILGQLSNDPVSRDLLKLNNIADTLDKSLGALDDIAKMLASQKDIMTEVPNIWPIKG
                     NLGHITMYFGQNENPFSTGQWYLHTGIDISTNRIGDTVLATADGKVIDIHYDSSLGNS
                     ITIQHSHGFTTRYGHLRSFAVKKGQRVSQGDVIGYLGNTGKTTGPHLHYEVYLGTSLI
                     DPLRFLSIRKEYHE"
     gene            1309..1647
                     /locus_tag="PK105_09415"
     CDS             1309..1647
                     /locus_tag="PK105_09415"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU61977.1"
                     /translation="MSRRDFSDCMIGEGAKVDGTIDAPGMVRIDGEFSGDIKSAASVI
                     ISKNAIVHAHINARQLLVAGIFTGSAIIEGGVRYAETARIEADCTGRFFVVEAGALVN
                     GQFSRIDAED"
BASE COUNT          510 a          344 c          451 g          477 t
ORIGIN      
        1 atgttgcggt tgaaaccggc aatttagtta ttcgtttata atatagcaaa tttttagcca
       61 aaggtgaatt cgggtaatgt tcgttttttg cttggctaca aacgcagaag tccgcgaata
      121 tctctttttg tgcgaatatc tctttttctg atcccaaata aaatgtcttt ttgttgattt
      181 gatagctaac tcgatcagct cgataaagta ctacattaat tgctcaaaat ttgcgataat
      241 ttataattag ggcaaggaga aaaagcaaag tgccagaagg cttgcgtaga atcaaagaga
      301 gagaaaagcg ttggtatggt cgtctggcgg agggtctagc aagttctttt attgctctac
      361 aagttgcact aaggcgcctg cttgcgtggg gaaggactcg cgtagttttt gctatgatcc
      421 cacaaagcga aagaccctct cggaagttcg agattcgccg tttttctctt ttggcaatag
      481 tcctgactat agtgatctgt attggagccg caataatttc aataggggcc tatagtacaa
      541 aatcagtaaa agtaatctct ctggaagcgc aactttccga tgcatataga agtatcgatc
      601 gattgcgcga atctagcgag aacctactat cggagacagc taaattcgag gaaaagcttg
      661 ggcaggtaat agagattgcg cagagcaaag gaaagcccgc agttcagcag agtacaagcg
      721 aagagatact caaggttgca ggcttggatg atccaatttt ggggcagttg tccaatgatc
      781 cagtgagccg cgatctctta aagctcaaca atatcgcaga taccttggac aagagcctag
      841 gagcgctaga tgatatcgca aagatgcttg cgagccaaaa ggacataatg acggaggtgc
      901 caaacatctg gccaatcaag ggcaatcttg ggcacataac catgtatttt ggacagaatg
      961 agaatccttt ttctacggga caatggtatt tgcacacggg tatcgatatt tcaactaacc
     1021 gaattggaga tacggtcctt gccacggcag acggcaaggt catcgacatc cattatgatt
     1081 cttctttggg caattccatc acgatccagc atagccatgg ttttacgaca cgatatgggc
     1141 acctccgaag ctttgctgtt aagaaaggcc agcgggtctc tcaaggcgat gtaatcggct
     1201 atcttggaaa tactgggaaa acaacagggc cgcatcttca ctacgaggta tatctgggta
     1261 caagccttat cgatcctctg cgttttctga gtatacgcaa ggagtaccat gagtagacgt
     1321 gatttttcag actgtatgat aggagagggc gctaaggtag atggaactat cgatgctcca
     1381 ggcatggtgc gcattgatgg agaattctcg ggtgatataa aaagcgctgc ttctgtgata
     1441 atctcgaaaa atgccatagt tcatgctcat ataaatgctc ggcagctctt ggtcgcaggc
     1501 atattcaccg gtagcgcaat tatcgaaggg ggagtgcgct atgcagagac cgctagaatc
     1561 gaagcggact gcacgggcag attttttgtc gtggaagcag gagctctagt aaatgggcag
     1621 ttttcgcgca tagatgctga ggattaatac tgaggagcta cataggcata aggtctgtga
     1681 ggctgcaatg ttgcgatatg ctttatatca ggagtaaata atgtctgttg aatataatga
     1741 tcatgagcta cctgaagata tactcgatat ccttggggag ga
//
LOCUS       DAOOWY010000228         1721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_69893_length_1721_cov_2.819328, whole genome shotgun sequence.
ACCESSION   DAOOWY010000228 DAOOWY010000000
VERSION     DAOOWY010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69893_length_1721_cov_2.819328"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..>1721)
                     /locus_tag="PK105_09420"
     CDS             complement(82..>1721)
                     /locus_tag="PK105_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOU61978.1"
                     /translation="FSVTIGKDGLAYLKDIGGGDEIPSELFSLKLISSKEELEQQIQA
                     NENECQICAGKSPDRYVILKFMSSDTISIECCGIGLRLDMHKSRYDFIQEMARGVFII
                     QSCAKDHVYELDLRYAVHAVDAPWGRVGNEYIRIDIFEGYATIREYALIPQESDAKKT
                     QFIGFSRWNQNYTGTELETRYSEARDLASYILWSAFVRPEGKLTRTSVYSSKNWMTNI
                     WSWDNCFCALAMVENMPNFALDQLLVFADNQHEAGVYPDFINDQFSLFSFCKPPIHGW
                     ALLEMIKRNSTILQSSIERIEYLYKSISDSTNFWLKYRRLPSGAIIYRHGNDSGWDNA
                     TLFMEGGPVESPDLYAYLSKQCDCLAEISRLLGHGIDEAENWTFLAESLANSMLDRLC
                     DDSGFFARRGLEGEKIEHRGSFLLRMPIVAYTRLPEKVIKKLILDISDENRFLTRFGL
                     ASEALDSPFIEPQGYWRGPIWAPVNYLVFDALRHLGEMSLANRIAQRFVDLCSEEGMA
                     ENFDPIDGKGLDDPAFSWTSAVFLLFLKESISKSIEN"
BASE COUNT          496 a          413 c          323 g          489 t
ORIGIN      
        1 gcaaaagcaa taaatccgct cgaattgcgg tcttgttcat ggtattagat tcctctatgt
       61 agggcttgat cgactctagt gctaattctc tatgctcttt gatatactct ctttgagaaa
      121 aagaagaaag accgcagatg tccaagagaa agcaggatcg tccaatccct taccatcgat
      181 agggtcgaaa ttttccgcca ttccttcctc tgaacataga tcgacaaacc tttgcgcgat
      241 tctgttcgct aggctcattt ctccaagatg ccttagcgca tcgaatacaa gataattgac
      301 tggcgcccat atcggacctc tccagtatcc ctgcggctct ataaaaggag aatccaatgc
      361 ttcgctagcc aaaccaaaac gtgtcaagaa tctattctca tcggaaatat ctaaaatgag
      421 cttcttgatt accttttctg gcaagcgcgt gtacgcaact ataggcatgc gaagaagaaa
      481 agagcctcta tgttctattt tttctccttc taatcctctt cgtgcaaaga atccactatc
      541 atcacaaagg cgatccagca tagaattagc aagcgattct gccagaaatg tccaattctc
      601 tgcctcatcg ataccatgcc ccagaagacg cgaaatctca gcaagacagt cgcactgttt
      661 agaaaggtat gcatataggt caggagattc gacaggtcct ccctccataa acagcgtcgc
      721 attatcccag cctgaatcat tgccatgccg gtagataatg gccccagaag gcaaccttcg
      781 atattttagc cagaaatttg tcgagtcaga aatcgactta taaagatatt cgatcctttc
      841 aatcgaggac tgaagtattg ttgaatttct ctttatcatt tccagcagag cccatccatg
      901 aatgggaggc ttgcaaaagc tgaataatga gaattgatcg ttgataaaat caggatagac
      961 tcctgcttca tgttggttat ctgcgaaaac caataattgg tctaaggcaa agtttggcat
     1021 gttttcaacc atggcaagtg cgcaaaagca attatcccaa ctccaaatat tcgtcatcca
     1081 attcttcgag ctatatacag aggtgcgcgt gagctttccc tcaggccgaa caaaagcaga
     1141 ccaaagaata tacgatgcca aatccctagc ttcgctatat cgagtttcta gctctgtccc
     1201 agtgtaattc tgattccatc tggaaaatcc aataaactgc gttttcttag cgtccgattc
     1261 ctgaggaatt aaggcatatt ctcgaattgt ggcatatcct tcgaagatat ctatccggat
     1321 atattcattt ccaaccctcc cccatggagc atcgacagca tgaactgcat atctcaaatc
     1381 gagttcgtag acatgatctt ttgcgcaact ttggattatg aatactcctc tggccatctc
     1441 ttgaatgaaa tcataacgtg atttatgcat gtctagcctt agacctatgc cacaacattc
     1501 aatactgatg gtatcagaag acatgaattt cagaataaca tagcgatctg gcgattttcc
     1561 ggcgcaaatt tggcattcat tctcattagc ctggatctgt tgctcgagct cttctttgct
     1621 cgagataagc ttcaatgaga acagttcact cggaatttca tctccccctc cgatatcctt
     1681 caaatatgca aggccatctt ttcctattgt tacagagaaa c
//
LOCUS       DAOOWY010000229         1690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_71644_length_1690_cov_17.686850, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000229 DAOOWY010000000
VERSION     DAOOWY010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71644_length_1690_cov_17.686850"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PK105_09425"
     CDS             <1..977
                     /locus_tag="PK105_09425"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOU61979.1"
                     /translation="DVKVKSADGYEKTFDLFKAERYGDLSQDPFLRPGDEIRISSLTN
                     LVTIQGEVKRPGTYQLLPGEGLKELVSVYADGFTEKANTSRLTLLHYLSESSPVGEKL
                     QFDYTKFPDMELRLYDVVTVPSLQELLPVAWFEGAVGLSSTGASPETSQRSAYTFVPG
                     ETVSQAALANRKLFSAVSDLASAYIIHTDGTQTAVNLAKFIYDYDLTNDIQLRANDII
                     IVPFRQFFVTVSGAVQFPGRYPYIPNRTWEYYIGLAGGFDTDKNSGQKITIYDVKSQK
                     VAIAGREIQPEDNIVAESNSFTYNMMKVSSIISTVLSFTVLIISLLGL"
     gene            1060..>1690
                     /locus_tag="PK105_09430"
     CDS             1060..>1690
                     /locus_tag="PK105_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61980.1"
                     /translation="MNYSNPELQLLQSIYRATEEGNSFASVSQRQLANSAGLSIGLTN
                     ILMKRFIERGWVKLLHIDGRKIKYALTPSGMEEIALRAVEYFARAERNIELYRKKIDF
                     FIEGIAHEGYEALLLVGLSELDFLFDYACLKQGMGFYKNMKVFMREIDAHDLAWEGVV
                     VVCSDNFEDDKDLQDDLPGDIKQHLGLPKEVPLIRFSQLLMNVVVKKSRF"
BASE COUNT          451 a          377 c          405 g          457 t
ORIGIN      
        1 gcgatgtcaa ggtcaaatct gccgatggct acgagaaaac ctttgatctt ttcaaagcgg
       61 agcgctatgg agatctttcg caggatccat tcctgaggcc tggcgatgag atcaggattt
      121 ctagccttac caatctggtg acaatccagg gtgaagtaaa acgccccgga acctaccagc
      181 ttctgcctgg tgagggtctc aaagagcttg tcagcgtata tgcggatggc tttaccgaga
      241 aagccaatac ctcgcgcctc acattgttgc attatctttc cgaaagctcg cccgtgggcg
      301 agaaactgca gttcgattac accaaatttc cagatatgga gctgaggctc tatgatgtag
      361 tcaccgttcc atccttgcag gagcttcttc ctgtggcatg gtttgagggc gcagtgggct
      421 tgagtagcac tggtgcatca cctgaaacca gtcagcgctc ggcttacact tttgtgcctg
      481 gcgaaaccgt gtcgcaagca gctctcgcaa accgcaagct attctcagct gtatccgacc
      541 tggcctccgc atatattata catacagatg gcacacagac agcggtcaat cttgcaaaat
      601 tcatctacga ttatgatttg acaaatgata tccaattgcg agctaacgac atcataattg
      661 tccccttccg ccagttcttt gtgaccgtat cgggtgccgt gcagtttcca ggccgctatc
      721 cctatattcc gaaccgcacc tgggagtact atatcgggct cgcaggcggc tttgataccg
      781 ataagaactc aggtcaaaag atcacaatct acgatgtcaa atcgcaaaaa gttgcgattg
      841 caggccgaga gattcagcct gaggataata tagtcgcaga aagcaattca tttacttaca
      901 atatgatgaa ggtttcttcg attatctcga ccgttctttc gtttacagtg cttataatta
      961 gcctattggg cttgtgaagc ttggctgttt catattccta ttaatctagg cctaattaat
     1021 gaatcgtctc gtattcttgt ctcataaaca gttacataga tgaattattc taatccagaa
     1081 ctccagcttc tacagtctat ctatagagct acagaagagg ggaatagttt cgcctccgtg
     1141 tctcaacggc agcttgctaa tagcgcaggg ctatctattg gattaacaaa tatccttatg
     1201 aagcgcttca tagagcgagg ctgggtcaaa ctccttcata tcgatgggcg aaagataaaa
     1261 tatgcgctta ctccttccgg catggaggaa atagcactga gagcggtgga gtattttgca
     1321 agggcagagc gcaatataga gctctatcga aaaaagatag acttcttcat agaaggaatt
     1381 gcgcacgagg ggtacgaggc gctgcttctc gtagggcttt cggagctcga ttttttattc
     1441 gactatgcgt gccttaagca aggaatggga ttctataaga atatgaaagt attcatgcga
     1501 gaaatcgatg ctcatgactt ggcttgggag ggagtggttg ttgtctgttc ggataacttt
     1561 gaagatgata aagacttgca ggatgatttg ccaggagaca taaagcaaca tctaggccta
     1621 ccaaaagagg tgccgctcat cagattttcg caacttctca tgaacgtggt ggtgaaaaag
     1681 agcagatttt
//
LOCUS       DAOOWY010000230         1690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_71656_length_1690_cov_3.924159, whole genome shotgun sequence.
ACCESSION   DAOOWY010000230 DAOOWY010000000
VERSION     DAOOWY010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71656_length_1690_cov_3.924159"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PK105_09435"
     CDS             complement(<1..691)
                     /locus_tag="PK105_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61981.1"
                     /translation="MQERSHSAFLLAAKPLLVDIMNRLEPNFDYVSVFAADDRGLSYI
                     ALPGETRTSEPRWVQRGFVFRAQRDGKVAEYACSSLPCYVSGPSGLASEAIKSPFLSE
                     PAAIGPPSSIATLAASIIQERLEILLADPTLIKFPKIPDDSATAEYRGEIEEDPFVAD
                     PESILSRLVALRERLTDGDIVVMAQARYEGVDVSRLFVSPNRSLIQSFMWSQAYLFGL
                     ARRGEISKMSYR"
     gene            complement(725..1327)
                     /locus_tag="PK105_09440"
     CDS             complement(725..1327)
                     /locus_tag="PK105_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU61982.1"
                     /translation="MLVPESFELTNIRLRRPRLSDAPAIFEYGSDPEVARLADWPILT
                     CLEPLLETLHRREVLWNEGIEYYWIITPKKPDKESDRAIGAISCRVAENVAENVAENG
                     AEIGYLLNRKYWGMGYATEAAKAVVDWVLSQPEISQVWATCDIENASSIRVLEKIGMH
                     REKILRNYSIRPQISCHPRDSYLFVRKGNTNTAKRKPYRI"
     gene            complement(1456..>1690)
                     /gene="glnQ"
                     /locus_tag="PK105_09445"
     CDS             complement(1456..>1690)
                     /gene="glnQ"
                     /locus_tag="PK105_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="similar to ATP-binding component of ABC
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HOU61983.1"
                     /translation="TSALDPEMIKEVLDVMTDLANEGMTMLVVSHEMGFARAAANRII
                     FMDAGQIVEEAPPEMFFTSPREERTRRFLEHIL"
BASE COUNT          444 a          400 c          394 g          452 t
ORIGIN      
        1 ggcggtaaga catcttgctg atttctccac gccttgccaa gccaaagaga taagcctgtg
       61 accacatgaa gctctggatg agtgaacgat taggtgaaac aaagagcctg gaaacatcga
      121 caccttcata gcgagcttga gccatgacca cgatgtcgcc gtctgtgagt ctttcgcgca
      181 aagccacaag gcgcgagaga atcgattcgg ggtcggcaac gaatggatct tcttcgattt
      241 ctccgcgata ttccgctgta gcggaatcat cggggatctt tgggaatttg attagagttg
      301 gatcggccaa tagaatttcg aggcgctcct gaattatgga agcagctaag gtggcaatag
      361 agctaggggg accgatagcc gcaggctcag ataagaatgg agacttaatc gcttcagaag
      421 ccagcccaga aggcccagaa acgtagcatg gaaggctgga acatgcatat tccgcaacct
      481 ttccatctcg ttgagctcta aaaacaaagc ctcgctgcac ccatcttggt tcgcttgtgc
      541 gtgtctcgcc gggtaaggca atatatgaaa ggccgcgatc atccgcagcg aacacgctca
      601 cataatcgaa attgggctcg agccgattca taatatcaac taatagcggc ttagccgcaa
      661 gcaaaaaagc gctatgcgat cgttcttgca taatactcct ctgtctgtca acaaaaatat
      721 ggatttaaat tctatatggt ttccgctttg ctgtatttgt atttcctttt ctaacaaata
      781 ggtaggaatc tcttggatgg cacgaaatct gaggccttat actgtaattc cgcaatattt
      841 tttctctgtg catgccaatt ttctccagca cacgaataga ggaagcattt tcgatatcgc
      901 aagtcgccca gacttgggat atctcaggct gagacaatac ccaatcgact acagctttag
      961 cggcttcggt tgcatagccc atgccccagt attttctatt gagtagataa cctatttccg
     1021 ctccattttc agctacattt tcagctacat tttcagcgac tcgacaggag attgcaccaa
     1081 tcgctctatc tgattcttta tctggctttt taggggtaat aatccaatag tattcgatgc
     1141 cttcattcca gagaacttct cgtctgtgca gtgtttcgag aagtggctct aggcaggtga
     1201 ggataggcca atcggcaagt ctcgccactt ctgggtcgct gccatactcg aaaatggctg
     1261 gtgcatcgct caatcgtggt cgccttagcc gaatgttggt cagttcgaag ctttctggta
     1321 caagcatctt ttcgcttcat tataccatta atggtacgaa ccttgtaaac gacaatgaag
     1381 ctgaaaggcg gaaaaaagag ctgataagaa atatagaaat cgaaggaaat aatatcgaaa
     1441 ttttctagtg gcttctcaca gaatatgctc caaaaacctg cgcgtgcgct cttcgcgtgg
     1501 cgatgtaaag aacatttctg gcggtgcttc ttccactatt tgaccagcat ccataaatat
     1561 gatgcgattt gcggcggcgc gcgcaaatcc catctcgtgc gataccacaa gcattgtcat
     1621 gccttcgttg gctaaatcgg tcattacatc gagcacttcc ttgatcatct ccgggtcgag
     1681 ggcgctggtt
//
LOCUS       DAOOWY010000231         1669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_72877_length_1669_cov_4.455390, whole genome shotgun sequence.
ACCESSION   DAOOWY010000231 DAOOWY010000000
VERSION     DAOOWY010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72877_length_1669_cov_4.455390"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PK105_09450"
     CDS             <1..461
                     /locus_tag="PK105_09450"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOU61984.1"
                     /translation="GVVFLLIGCSNVFNWALVVEQIPQSIALFIGKTITNKYLLLLLI
                     NIFLLIVGCFMEGTAAIIILSPLLLQITQPFGISPIMLGTLIVLNLMIGLVTPPVGLC
                     LFIACNIAKRPIEAVSKALVPFLIASIITLLLVTYFEPLSMWLPRLLGYA"
     gene            538..>1669
                     /locus_tag="PK105_09455"
     CDS             538..>1669
                     /locus_tag="PK105_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOU61985.1"
                     /translation="MAEPQKFANFINGQWKEPQGGNYYPDINPADYSDIVGYFPLSTK
                     NDVDEAIQAAHIAFKSWSHMLPAERAKYLEKFAQLLDQEKQRIGEALCREEGKTIKEA
                     VGEPARGAVETSFFIGEGQRLEGITMPSDRKGVVSVATRVPIGVVAAISPWNFPFLTP
                     VRKVIPALVFGNTVVFKPASDTPYCAVLLMELFEKAGLPPGVVNLVIGKGSAIGDAIS
                     SNPLVKGITFTGSTVVGRRINQLASEHFAKVQLEMGGKNPAIVADYKDLDRAAAEIAS
                     AAYAVSGQRCTAVSRVIVLKKHAEELEAKIIEKAKGYVLGNGMRPEVTMGPIITKEAG
                     EKIMEYIHGAEKEGATIKYGGRHLTGGEFDKGFYIEPTLITDV"
BASE COUNT          455 a          352 c          418 g          444 t
ORIGIN      
        1 cgggtgtcgt atttcttctt attggctgtt ccaatgtgtt taactgggct ctcgtagtcg
       61 aacagattcc tcaaagcata gccttattca ttggaaagac aataacaaat aagtatcttc
      121 ttctgttact tataaatata tttcttttga ttgtgggttg ctttatggaa ggaactgccg
      181 cgatcatcat tctatcgcct ctattgcttc agattactca gccattcgga ataagcccta
      241 taatgttggg aacacttatt gtactaaacc tgatgattgg attggtgacg cctcctgtcg
      301 ggctatgtct ttttattgct tgcaatattg caaagaggcc tatcgaggct gtcagcaagg
      361 cgcttgtgcc tttcttgatt gcatcgataa tcacactctt attagttaca tactttgagc
      421 cgctttcaat gtggttacct cggttgcttg gctatgcgtg attaggaatc tacagatggc
      481 ggattccaag gatagcgcat tcaaaagaga gcgcattatt tgaaaggaga attggatatg
      541 gccgaaccac agaaattcgc caattttata aatggccaat ggaaggagcc gcagggggga
      601 aattattatc ccgatataaa tcccgcggat tatagcgata tcgtgggata cttccccctg
      661 tctacaaaaa atgatgtcga tgaggcaata caagcagcgc atatcgcttt taagagttgg
      721 tcgcacatgc ttcctgctga gcgcgcaaag taccttgaaa aatttgcaca gcttcttgat
      781 caagaaaagc agcgaatcgg agaagctttg tgtcgtgagg aaggcaaaac gataaaggaa
      841 gccgtaggag aacctgcgcg aggtgcggtt gagacttcat tttttatcgg agaaggccag
      901 cgacttgaag gcatcactat gcccagcgac cgcaagggcg tagtttcagt cgctactcgc
      961 gttccgattg gtgtggtcgc tgcgatttcg ccatggaatt tcccctttct tacccctgtg
     1021 cgcaaggtta ttccagcgct tgtattcggc aatacagtcg tctttaagcc agcttcggat
     1081 acgccatatt gcgcggtgct tctcatggag ctcttcgaaa aagctgggtt gccacctggt
     1141 gtggtcaatc tcgtcatagg aaaggggagc gcgattggcg atgccatttc ctccaatccg
     1201 cttgtaaaag gtataacctt tacaggctca acagtcgttg gacgaagaat caatcagctt
     1261 gcctctgagc attttgcaaa ggtccagctt gaaatgggag gaaagaatcc agcgattgtg
     1321 gcggattata aagatctcga tagagcagca gcagaaatag cctcagcagc ctatgcagtg
     1381 agtggacagc gctgcacagc agtgagccgt gtcattgtgc taaagaagca tgcagaagag
     1441 ctagaagcga agatcatcga aaaagcaaaa ggctatgtgc tcgggaatgg aatgcgacca
     1501 gaagtgacca tgggccccat tatcacaaag gaagctggcg aaaaaataat ggagtatatc
     1561 catggcgctg aaaaagaagg agcgacaatc aaatatggag gccgtcatct tactggtggt
     1621 gaattcgaca aaggcttcta tattgagccc actttgatca cagatgtca
//
LOCUS       DAOOWY010000232         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_73053_length_1666_cov_11.617629, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000232 DAOOWY010000000
VERSION     DAOOWY010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73053_length_1666_cov_11.617629"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /gene="hisF"
                     /locus_tag="PK105_09460"
     CDS             complement(<1..726)
                     /gene="hisF"
                     /locus_tag="PK105_09460"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOU61986.1"
                     /translation="MSGLAVRIIPCLDFKDGRVVKGTQFQNLVDSGDPLERALVYEKQ
                     GADELTFLDISATLEGRKTMAQEVVHIRQALSIPITVGGGIGSIEDAGRLLEAGADRV
                     SVNSAAVRNPMIIDELASRFGVQCVVVAIDAAQNATMPSGYEVKIDAGSRKTGLDAIA
                     WAQEATDRGAGEILLTSIERDGTSLGYECKLIGLVAHAVLVPVVASGGASKPEHFLEG
                     YLSGASALLAAGIFHRGEVSIREI"
     gene            complement(723..1421)
                     /gene="hisH"
                     /locus_tag="PK105_09465"
     CDS             complement(723..1421)
                     /gene="hisH"
                     /locus_tag="PK105_09465"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOU61987.1"
                     /translation="MGERTEVVIIETGVANLASVKAFAARLGLKPIVSDQIVDVLEAP
                     AVILPGVGAFGPAMERLSLLRLDEALRRRVEKGLPTAGICLGMQLFFEASEESPGIQG
                     LGIFKSNVKKLVGGLALPQLGWNRIEPESTARLIESGWVYFANSYRVGEIERTSKVSI
                     KGPVDSFMAQKIFDQVAIATAIYGEPFIAALEAWKDTRPTLLLCQFHPELSGPFGTQL
                     FSHWLNRNRIRRNV"
     gene            complement(1424..>1666)
                     /locus_tag="PK105_09470"
     CDS             complement(1424..>1666)
                     /locus_tag="PK105_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61988.1"
                     /translation="PSIGSLSTCNIEHFFQSFTTNARCTLHLDILRGENDHHKAEAVF
                     KAMALACRAALTPQPIFSRNTSGSTKGEASIDLEHA"
BASE COUNT          383 a          464 c          410 g          409 t
ORIGIN      
        1 tatctctcga atcgagactt caccgcgatg gaaaatccca gcggcgagca gagcagatgc
       61 gccacttaaa tatccctcca gaaaatgctc tggcttcgaa gctccccccg aagcaaccac
      121 cggcacaagc acagcgtgcg cgacaaggcc tatgagcttg cattcatagc caaggcttgt
      181 gccatctctc tctatcgaag tgagaagaat ctcgcccgcg cctcggtcag tagcctcctg
      241 agcccaggca attgcatcga gaccggtttt acgagaacca gcatcgatct taacttcata
      301 tcccgaaggc atggtagcat tctgcgctgc atcgatggct acaaccacgc actggacgcc
      361 aaagcgcgaa gcaagctcat cgataatcat aggattgcgc accgctgcgc tgttcacaga
      421 gacccggtct gctcctgctt caaggaggcg gcccgcatcc tcgatgcttc cgatgccgcc
      481 tcccactgtg atgggaatcg agagcgcctg tcgtatatgc accacttcct gcgccatagt
      541 ctttctgccc tccagagtgg cgctgatatc gagaaaggtg agctcgtccg ctccctgttt
      601 ttcgtacacg agagcgcgtt caagggggtc tcctgaatcg acaaggtttt gaaactgtgt
      661 tcctttgacg acgcgtccat ccttaaaatc gaggcatgga attattctca ctgcaagtcc
      721 gctcatacat ttctccgaat cctgtttcga ttgagccaat gcgaaaaaag ctgtgtacca
      781 aagggtccgg aaagctctgg atgaaactgg cagagaagaa gtgtgggcct tgtgtctttc
      841 caggcttcca gcgcagcgat gaatggttcc ccatagatgg cagtcgcgat ggccacctga
      901 tcaaaaattt tttgcgccat gaaggaatct acaggccctt ttatggatac cttgctagtg
      961 cgctcaattt ctcccacccg gtagctattc gcaaaataga cccagccaga ttcgataagg
     1021 cgcgcagtgc tttcaggttc aatcctattc catccaagct gaggcaaggc taggcctcct
     1081 actagttttt ttacgttgct cttgaaaata ccaagccctt gaattccagg cgattcttcg
     1141 ctcgcttcga aaaagagctg catgccgaga catatgcccg ctgttggaag ccctttctcg
     1201 acacgcctgc gaagagcctc gtcaagcctc aggagagata gcctttccat tgctggtcca
     1261 aaagctccta ctcccggaag gatgacagca ggagcttcga gtacatcgac tatctgatcg
     1321 ctcactatgg gtttgaggcc taaccgcgct gcaaatgctt ttactgaggc aagatttgct
     1381 acccctgtct caataatgac gacctctgtt ctttctccca tgattatgcg tgctccaaat
     1441 cgatgctggc ttctcccttg gtgcttccgc tcgtgtttct gctgaaaatc ggttggggtg
     1501 tgagcgcggc cctgcaggca agcgccatgg ccttgaagac tgcttctgcc ttgtggtggt
     1561 cgttttctcc gcgaagaatg tcaaggtgaa gcgtacatcg tgcgttcgtt gtaaagctct
     1621 gaaaaaagtg ctcaatattg caggtcgaaa gcgagcctat agatgg
//
LOCUS       DAOOWY010000233         1665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_73111_length_1665_cov_37.375776, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000233 DAOOWY010000000
VERSION     DAOOWY010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73111_length_1665_cov_37.375776"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PK105_09475"
     CDS             <1..1235
                     /locus_tag="PK105_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOU61989.1"
                     /translation="SAAATVLDGYPLAKKGEAGKAMGVALVASTLGGIFSTICLIFLA
                     PQLAKIALKFGPPEYFALMIFGLTIMASVSSASLTKGFLSGFFGLLLSMVGLNPTLGT
                     ERFTFGITRLLTGFSMMPVLIGLFAVSEVFAQSELLSEGKIVQLSKKHKIGSIMPTKK
                     EMKGLWKVILGSSVLGTIIGIIPATGGSIASFMAYNEAKRFSKDPDSFGTGNLAGIAA
                     PESANNATTGGAMIPLLTLGIPGDVVTSVMLGALMIIGVQPGPLLFSQSPQIVTDLFV
                     GFMMAQFIMLALGYLSIRASNLILRVPLGILYPTILCLCLLGAYSLGNSVYDVMLALI
                     FGIVGYFIKKYGFSAPSIILGLILGPIAEQELSRALIISHGDWTVLVRSPLAILFYVF
                     AVASIIYSFRSFKKSKAK"
     gene            complement(1351..>1665)
                     /locus_tag="PK105_09480"
     CDS             complement(1351..>1665)
                     /locus_tag="PK105_09480"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOU61990.1"
                     /translation="YRHKVEINAELDFHRSIAEATRNPVIMRIIPIIMESIEKTYHDA
                     PRTSEDHRHALEEHKVVYEAIAAHDPDRSFDAMQCHLEASYKRTLARQVPPAKDKEDK
                     PS"
BASE COUNT          371 a          389 c          384 g          521 t
ORIGIN      
        1 catctgcggc agcaacagtg ctcgatggct atccacttgc caagaaggga gaggcgggaa
       61 aggctatggg tgttgccttg gtcgcctcga ccttgggcgg catcttctcc acgatttgcc
      121 taatctttct cgcgccacag ttagcgaaaa ttgcccttaa attcgggcca cctgaatatt
      181 ttgcactaat gattttcggc ctcacaataa tggctagcgt ctcttcggcg tctctgacta
      241 agggttttct atcgggcttt tttggacttc tactttcgat ggtcggctta aaccccacct
      301 tgggcaccga gcgctttact tttggtataa caaggcttct cacgggcttt tccatgatgc
      361 ctgtgctcat cggtctcttt gcagtttccg aagtctttgc ccagtcggaa cttctaagcg
      421 aaggaaagat tgtccagctt tcaaaaaagc acaaaatcgg atccatcatg ccgactaaaa
      481 aggaaatgaa gggtctctgg aaggtgattc tagggtcttc ggtcctaggg actataatag
      541 ggataattcc tgctaccggt ggttctattg cctcttttat ggcttacaac gaagcaaaac
      601 gattctcgaa ggatcccgat tcctttggta cgggaaatct tgccgggata gcagcgccag
      661 aatcggctaa caatgcgact actggtggag cgatgatacc tcttctcacc ttaggaattc
      721 ctggagatgt cgtaacctct gtcatgctgg gcgctcttat gatcattgga gtgcaaccag
      781 gaccccttct tttctcgcag tcgcctcaaa ttgtcaccga tctgtttgtc ggattcatga
      841 tggcacaatt catcatgctg gctttgggtt atctcagcat tcgcgcttcc aatctaatct
      901 tgagagtacc tcttggtatt ctctacccta cgattttatg tctatgtctt ttgggagctt
      961 attctctggg caacagcgtc tacgatgtca tgcttgctct tatattcggc attgtcggct
     1021 attttattaa gaaatatgga ttctctgcac cgtccattat attggggctc attctcggac
     1081 ctattgcaga acaagagctc agcagggcgc tcattatatc tcatggggat tggactgtcc
     1141 ttgtgcgttc gccgcttgcg attttgttct atgtctttgc agttgcctcg ataatctact
     1201 cttttagaag cttcaaaaag agcaaggcca agtagatatc aacaaatcga tattaagatt
     1261 gaaagtttgg ctccaagagg ccagatgggt ctaaacggct gtttggcagt gggcgagtct
     1321 ttactagctc gatttatttt tttatctttt ttagctcggc ttatcttctt tatctttagc
     1381 aggaggtact tgcctcgcca aggtacgctt atagctggct tcgagatgac actgcatggc
     1441 atcaaaagat ctatctggat catgagcagc gatagcctcg tagactactt tatgttcttc
     1501 caaagcatgc cgatgatctt cgctcgtccg cggtgcgtcg tggtaggtct tttcaatcga
     1561 ttccataata ataggaatga ttcgcattat tacaggattt cgagtagctt ccgcaatgct
     1621 cctatggaag tcgagctctg cgtttatctc tactttatgc cgata
//
LOCUS       DAOOWY010000234         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_73378_length_1661_cov_6.990037, whole genome shotgun sequence.
ACCESSION   DAOOWY010000234 DAOOWY010000000
VERSION     DAOOWY010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73378_length_1661_cov_6.990037"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PK105_09485"
     CDS             complement(<1..376)
                     /locus_tag="PK105_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU61991.1"
                     /translation="MLFVEGFSLFDVFYLADPIDIANTIRQVVGLISIERSIDRKALV
                     DALLSREEVMSTAIGNGIAIPHVRLFDSMSLQEDIVAVAYLFEPVDWVAPDGKPVHTL
                     FFVLAADETRHLQILAEIAQLAS"
     gene            complement(565..>1661)
                     /locus_tag="PK105_09490"
     CDS             complement(565..>1661)
                     /locus_tag="PK105_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61992.1"
                     /translation="PTLSASSTLSWGWDLDTGITGFKNANTANISVLFPVENASKKGD
                     TGWWGEIAVNNLYFEISDEQLTFNDPLLFTDWNDLDGDGTRDSGEYATLSAKITNSVW
                     ALSVSTWNKFDFANADALYDGDVAVTIDADTAGTSLSYTANGLTAAVTATSKGDWIAN
                     AANEYALGANASYKLSDALTVSGGIAYDAFDASKQFGGTASVAYSAAPLTVSIATDLF
                     YVPTPSSFDADALLTVGYAVMEGLDAEADAYYSTGDDDVEAKVAVDYEVDPIEAGISL
                     GVYDPISAIAYDLEIYAGYTYAIDPATKLYGYAKYTSDFTGKGSLAPYVSLTNTSVTN
                     TTLKLEYNPDDADVLAGKYGKIIASAKISL"
BASE COUNT          467 a          406 c          368 g          420 t
ORIGIN      
        1 cggaagcaag ctgagctatc tccgcaagaa tctgcaaatg ccttgtctcg tctgcagcaa
       61 gcacaaaaaa gagcgtatgt acaggctttc catcaggagc tacccagtcc acaggctcga
      121 ataggtatgc gaccgcgact atgtcttctt gaagactcat gctgtcgaac aatctgacat
      181 gcggaatcgc aattccattc ccaattgccg tgctcatcac ctcttcgcgg gagagaaggg
      241 catctaccag agccttgcga tcgatgcttc tctcaataga aatcaaccct acaacctgcc
      301 taattgtatt agctatatcg ataggatcgg ctaggtaaaa gacatcaaag agagaaaagc
      361 cctctacaaa cagcatgata ttctcgcgtt ttgcttgttt tgcaaattca taagaatgcc
      421 atggctaagc acaattttta ctaccataca ttcaaaatag cctttttcgg cctttttcat
      481 cgcattgctt atcacaatag tatttcccaa aaaaggccgc cccgagggcg gcctttgtct
      541 tgagctttat ccaattctag aggattatag agaaatcttg gcagaggcta tgatttttcc
      601 atacttgccc gctagcacat cggcatcatc cggattgtac tcgagtttga gagtagtatt
      661 ggtaacagaa gtattggtca gactaacata tggtgccaat gaaccttttc ctgtgaagtc
      721 gctcgtatat ttagcataac catagagctt cgtcgctgga tcaatagcat aggtgtatcc
      781 agcatagatt tccaagtcat aggcaatggc gctaatggga tcataaacac caagcgatat
      841 accggcttcg atgggatcta cttcataatc aacagctact ttagcttcga catcgtcgtc
      901 tccggtgctg taataagcgt cagcttcagc atccaggcct tccattacag cgtaaccaac
      961 agtgagaagc gcatcggcat cgaagctact tggagttgga acatagaaaa gatcagttgc
     1021 gatagataca gtgagcggag ctgcggagta ggcaacagaa gcggtcccac cgaactgctt
     1081 gcttgcatcg aaggcatcat aagcaatgcc accactcact gtgagcgcat cgctcagttt
     1141 ataggaagca tttgctccaa gagcatactc gttcgcagca ttcgcaatcc aatcgccttt
     1201 agaagtagca gtgacagcgg cggtgaggcc attcgcagta taggaaagag aggtgccggc
     1261 ggtatcagca tcgattgtga cagcgacatc gccgtcatag agcgcatccg catttgcaaa
     1321 atcgaatttg ttccaggtgg aaacgctcag agcccacacg ctattggtga tcttggcaga
     1381 tagagtggca tactcaccag aatctctagt cccatcgcca tctagatcat tccagtcggt
     1441 gaacaaaagt ggatcattga aagtcagttg ttcatcactg atttcaaagt atagattatt
     1501 taccgcaatc tctccccacc agcctgtatc acctttctta gaagcattct caacaggaaa
     1561 aagcacggaa atattcgcag tatttgcatt tttgaaacca gtaatccccg tatccaaatc
     1621 ccagccccag ctgagtgtgg aactcgctga gagggtcgga g
//
LOCUS       DAOOWY010000235         1655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_73763_length_1655_cov_20.131875, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000235 DAOOWY010000000
VERSION     DAOOWY010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73763_length_1655_cov_20.131875"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..481)
                     /gene="bfr"
                     /locus_tag="PK105_09495"
     CDS             complement(14..481)
                     /gene="bfr"
                     /locus_tag="PK105_09495"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOU61993.1"
                     /translation="MKGDPKVIDVLNSLLAEELTAINQYMVHSEMCDDWGYEKLHKSI
                     EKRAIDEMKHAEKLIGRILFLEGTPIVSKYNEIHIGADVSEMYKNDHLAEADAIKSYN
                     SAIALCGEARDYATRDILEAILKDEDAHIDNIEAIQDEIEQMGIQVFLSMQLG"
     gene            complement(742..1389)
                     /locus_tag="PK105_09500"
     CDS             complement(742..1389)
                     /locus_tag="PK105_09500"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOU61994.1"
                     /translation="MEQQAVRMPLLGDSFPQVSLQTTQGMLNLPGDLKGSWFILFSHP
                     ADFTPVCTSEFVAFQKLIPEFEKLGVKLIGMSIDQITSHIKWLEWIKEKLGVEITFPL
                     AAANDSIAMTLGMLHPGKGTNTVRAVYIVDPNGIIRLILYYPQEIGRFIPEVLRAVKV
                     LQISDKNHVSMPANWPDNELIGDHVIVPPPKTVAEAADRLQHYEGFDWWFCHKSL"
BASE COUNT          467 a          373 c          335 g          480 t
ORIGIN      
        1 aatatcgcaa gagctagcct agttgcatgg aaaggaaaac ctgtatgccc atctgctcta
       61 tttcatcctg tatggcttca atattatcga tatgggcatc ttcgtccttg agaatcgctt
      121 ctagaatatc gcgcgtagca taatcccgag cttctccaca aagggcaatg gcagaattat
      181 agctctttat cgcatctgct tccgccaaat gatcgttttt atacatctcg gatacatctg
      241 caccaatatg gatttcgttg tacttactga caattggcgt gccttccaaa aaaagaatac
      301 gaccaatcag cttctctgca tgtttcatct catcgatagc tcgtttctca atggacttat
      361 gaagcttctc atatccccag tcatcacaca tctcggaatg aaccatgtat tgattgatag
      421 cggtcaattc ttctgctaga agggaattga gcacatcgat tactttggga tctcctttca
      481 ttgttgtacc tcctccaaat ttccattatt tgagcttgtc ctttaaaagt atcatataca
      541 gccaatcaga ggccgtaaat agtgagaagc gtgatatgca ccctcagaat taataaagcg
      601 aggttagctt tttcgcaaaa ttatcgcgga tccttaaggc agagcgcata ctcgagcact
      661 ctgcctaaca tgcctaacaa tagcacgatt gatactggac cttcctaata catgcgggct
      721 tattgaccct tcgacgattt tctatagcga tttgtggcag aaccaccaat caaaaccttc
      781 ataatgctga agacgatccg cagcctcggc aactgtcttt ggtggcggaa caatcacgtg
      841 gtcgccaatt agctcattat ctggccaatt tgccggcata ctgacatgat tcttatccga
      901 aatctgtaat acctttaccg ctcgaagaac ctcaggtata aagcgtccta tttcttgtgg
      961 ataatagagt ataagacgaa taatgccatt tgggtctaca atgtagaccg cccgcacggt
     1021 attggtgcct ttccccgggt gaagcatacc tagagtcatt gcgatagaat cgtttgctgc
     1081 ggcgagaggg aaagttatct cgacaccgag tttttccttt atccactcca gccatttgat
     1141 atggctcgtt atctgatcta tcgacattcc aatcagcttg acgccgagct tttcgaattc
     1201 tggaattagt ttttgaaaag ccacaaattc acttgtgcac acaggcgtaa agtcagcagg
     1261 atgactgaaa agaatgaacc acgaaccttt gagatcgcca ggtagattta gcattccttg
     1321 cgtagtctga agactgactt gaggaaaaga atcccctaaa agcggcatac gtactgcttg
     1381 ctgttccata gcaaactcct tttaagacaa gatttctgca ttgtgatgca ccctactctc
     1441 tttgtaagaa taggcacaag aatctaaagc aagaatagtg ccaattaact gcaaaagaat
     1501 aataaagctg cttgaatatc taagtcctta tcggtcaatg ctttttcaga ttgctctcat
     1561 tgactgatgg gtggatggac aggataaaag gagagagttg tcttgacagc accgaaatat
     1621 cggtattaaa tgactgtatg tcgttggata ctgat
//
LOCUS       DAOOWY010000236         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_73824_length_1654_cov_14.281426, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000236 DAOOWY010000000
VERSION     DAOOWY010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73824_length_1654_cov_14.281426"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1654)
                     /locus_tag="PK105_09505"
     CDS             complement(<1..>1654)
                     /locus_tag="PK105_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOU61995.1"
                     /translation="MDLYAHEPLGFDGILVHIEADIRNGIPAVEIVGLASTSVREARE
                     RARIAVRNAGFEFPQDRILVNLSPADLPKEGSAYDLPIALKILEKSGQIGDLPICAMA
                     MGELTLEGEIRPVRGILPAVRSAASHGIRICILPFENAQEARILKAVQVWPIRHLAES
                     NRILIEIGKGKTPQGDVQEGQFSQGDLPIGQEKQIPPRSPSLWLDLNESRETYSDFRG
                     DERLMRALVIAAAGGHNMFLAGPPGAGKTMAARRFPSILPDLSEQEALDTASIYSLWG
                     SLWTCSGRRPPFRAPHHSASMEGILGGAKPLRPGEVSLAHHGVLFLDECPEFRRDVLQ
                     GLREPVERGYVDIVRAGRVLRFPSDFQLIMAANPCPCGNLGMRGKTCICSPEEIRRYW
                     KKLGGPLLDRIDMRIAIQPPKASRLIESRAVDVDLLRDKVKSAREIQNMRFKRTNDRM
                     SKKETENMGSSRSMMFSEANQILTCNNAHIPAGIVNDICHIAGSAEKAFLSGMANYSL
                     SARAGHSILRLARTIADIDGENEITESAIEEAIEYRQFGDGDA"
BASE COUNT          412 a          432 c          375 g          435 t
ORIGIN      
        1 gcatctccat cgccaaactg tcgatactcg attgcttctt ctattgcaga ctctgtaatt
       61 tcgttctctc catctatgtc ggctatggta cgtgcgagtc taagaatgct atgcccagcg
      121 cgtgccgaaa ggctataatt agccatccct gatagaaatg ctttttcagc cgatcctgct
      181 atatgacaaa tatcatttac aatccctgca gggatatgag cattgttaca agtgagaatt
      241 tgatttgctt ctgaaaacat catgcttctt gaagacccca tgttttccgt ttctttctta
      301 ctcatcctat cgtttgtcct tttaaaccgc atgttctgga tttcgcgcgc agattttact
      361 ttatctcgaa gcaaatctac atctaccgct cgagattcga taaggcgaga agctttcgga
      421 ggctggattg cgatgcgcat gtcgattcta tccaggagag gtcctccgag ctttttccaa
      481 tatcgcctaa tctcttcggg agaacatatg caggtttttc cgcgcatacc caaattgcca
      541 caaggacaag gatttgcagc cattatgagc tgaaaatctg aaggaaagcg aagcactcgg
      601 ccggctcgca caatatcgac ataaccacgc tcaacaggct ctcggagtcc ctgcaagaca
      661 tcccgtctaa actctgggca ttcatcgaga aaaagcacac catgatgagc cagactgacc
      721 tctcccggcc tcaggggttt cgcgcctccg agtattcctt ccatgctggc ggagtggtga
      781 ggagctctaa atggcggcct ccttcccgag caggtccaaa ggctgcccca aagagaatag
      841 atggaagcgg tatcgagagc ttcttgctcc gaaagatctg gaagaatcga aggaaagcga
      901 cgggcagcca tggtcttccc cgcaccaggc ggtccagcga gaaacatatt atgccctcct
      961 gcggccgcaa tgacgagcgc acgcatcaat ctctcatcac cgcgaaaatc gctatatgtt
     1021 tccctgcttt cattcagatc gagccacaag gagggagacc ttggcgggat ttgcttttcc
     1081 tgtccaattg gcaaatctcc ttgtgaaaat tgcccttctt gcacatctcc ttgtggcgtt
     1141 tttccctttc caatttctat aaggattcta tttgattcag ctagatgacg aataggccac
     1201 acctgaacgg ccttcaaaat ccgtgcttct tgggcattct caaaggggag gatacaaatt
     1261 cgaataccat gagaagcagc agagcgaaca gcgggcaaga ttcctcttac gggccgaatt
     1321 tctccttcca gcgtcaattc tcccattgcc atcgcacata tgggcaagtc ccctatctga
     1381 ccagattttt cgagtatttt taaggcaatg ggtaaatcat aggctgagcc ctctttgggc
     1441 aaatcggcag gagagagatt tacaagaata cgatcctgag ggaattcgaa gcctgcattc
     1501 ctaactgcga ttctagcgcg ctcgcgtgct tcgcgcacag atgtcgatgc gagaccaact
     1561 atttcgacgg cgggtatgcc gtttctaatg tcagcctcga tatgaactaa gattccatcg
     1621 aatccaagcg gttcatgtgc atagagatcc atgc
//
LOCUS       DAOOWY010000237         1550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_80816_length_1550_cov_4.911706, whole genome shotgun sequence.
ACCESSION   DAOOWY010000237 DAOOWY010000000
VERSION     DAOOWY010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80816_length_1550_cov_4.911706"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PK105_09510"
     CDS             <1..313
                     /locus_tag="PK105_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOU61996.1"
                     /translation="FLSRLGFSEEIQKDVKFLIRYHMMPAALPILPVQKTEELIKDPR
                     FPVLLELFRCDEFSSFKDAERYYDACNMYQSIRRNLRNPYRKADGSKVYASRRSGSHF
                     S"
     gene            complement(308..1522)
                     /locus_tag="PK105_09515"
     CDS             complement(308..1522)
                     /locus_tag="PK105_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU61997.1"
                     /translation="MKRKLFVPIVITLVSLLFIGIFIYSIQRHIEGRNFQSVNNALSS
                     LIEIQASALYNGCFSHNQIYNLVIEGELADANALVQKATDSFSLIDLTEIVEGKPPAD
                     GFNVILDGELLRIECPIRNGDETATVPNVVGVAIIQAQRLLNTVSAKDFRIDLAHGRK
                     TIYGIPISANFTGHRLLDSIIIALLIGFIAVFIFQHYYRKANTFLDTRGLESIIYLFE
                     QTERISASHSRNVALLALFVGKKLGFKGRRLKDLYIAALLHDIGKIGIPNNILTKNGK
                     LDSCEFVQMRQHPTISARILRSFKEFERLSPIVLYHHEREDGSGYPEGLIGKEIPLES
                     KIIAVVDVFEALVGERPYRDPICPLSAFQKMKSMPLDQKIVNLLMDHYGELVAFKPPR
                     WTLSYSPWILAS"
BASE COUNT          450 a          345 c          320 g          435 t
ORIGIN      
        1 atttctttct cgtttaggct tctccgaaga aatccaaaaa gatgtcaaat tccttatcag
       61 atatcacatg atgccagccg cactaccgat acttcctgtt caaaagacag aggagctgat
      121 aaaagatccc cgcttcccag ttttgctaga gctatttcgc tgtgatgaat tctcaagttt
      181 caaggatgca gaacgatact acgacgcatg caatatgtat caatcaattc gacgcaatct
      241 gagaaatcca tatagaaagg ccgatggatc caaagtttat gcttcaaggc gctcgggttc
      301 gcacttttca tgaggcgagt atccatgggc tataagacaa agtccatcgc ggtggcttaa
      361 aagcaactaa ctctccatag tggtccataa gcagatttac tatcttttgg tcgagtggca
      421 ttgatttcat cttctggaat gcgcttagag gacatattgg atctctataa gggcgctctc
      481 ctacgagggc ctcgaacaca tcgactactg cgattatctt tgattccaag ggaatctcct
      541 tgcctatcaa gccctcagga tatcccgaac catcttctct ctcgtggtga tagagcacta
      601 tggggctaag tcgctcgaac tccttgaaac tcctgagaat acgcgctgaa atcgtagggt
      661 gctgtctcat ttgaacaaac tcacatgaat cgagcttacc gttcttggta agaatgttgt
      721 ttggtatgcc tattttgccg atatcatgga gaagtgcggc aatgtaaagg tccttgagcc
      781 ttctgccctt aaaaccgagt ttcttcccga cgaaaagagc aagcagggca acattcctag
      841 aatggctcgc agagattctt tcggtctgct cgaagagata gattatggat tcaagcccgc
      901 gagtatccag aaaagtgtta gctttcctat aataatgctg aaaaatgaaa accgcgataa
      961 atcctatcag tagtgctatg atgatggaat ccaataagcg atgccctgtg aaattggcgg
     1021 atattggaat tccataaatg gtttttctgc catgcgctaa atcgattctg aaatccttag
     1081 ctgagacagt attcaatagt ctctgggcct ggataatcgc cacgccgacc acatttggaa
     1141 cagtagccgt ttcgtcgcca ttgcggattg ggcattctat gcgcaatagt tctccatcga
     1201 gaatgacatt aaaaccatct gctggtggct tgccttctac aatttctgtc agatctatca
     1261 aggaaaaaga atccgttgct ttctgtacaa gggcatttgc gtccgcaagt tccccttcta
     1321 taactaagtt atagatctgg ttatgtgaaa aacaaccatt gtaaagcgca gacgcttgta
     1381 tctcgatgag actcgaaagc gcattattga cagattggaa gtttcgacct tcaatatgcc
     1441 tctgaataga atagataaat attccgataa aaagcaaact taccagtgta atcacaatcg
     1501 gaacaaacaa ctttcttttc atacaacatt tcctaataat aactaacaaa
//
LOCUS       DAOOWY010000238         1544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_81273_length_1544_cov_11.297515, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000238 DAOOWY010000000
VERSION     DAOOWY010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81273_length_1544_cov_11.297515"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PK105_09520"
     CDS             complement(<1..298)
                     /locus_tag="PK105_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU61998.1"
                     /translation="MIIGERMNDPNVMYLVDPEDKAKALNKLIGSAPLPNSLDREALF
                     DALLSREDMMSTAIGNGIAIPHVRLFDSMSLQEDIVAVAYLFEPVDWVAPDGKPV"
     gene            complement(447..>1544)
                     /locus_tag="PK105_09525"
     CDS             complement(447..>1544)
                     /locus_tag="PK105_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU61999.1"
                     /translation="PTLSASSTLSWGWDLDTGYTGFKNKLSATVTIPFAVEDAKKAGE
                     TGWWGEIAVTNITFKLTDDVLNASVLGFTDTDDDGNPASLSAKITNGIWAVSVDSYSA
                     LGYNNAKAIKDGDVNTTALIDANKFGTSVSYNDGTLGFGATAASKDDWTANAANEYAL
                     GANASYKVSDALSLSGAAAYDAFDATKDFAGTAKVSFAQGPLSVSVASDLQLVSAKSK
                     FDADALLKISYTVIEGLKTGLDGYYSTLDKDVEAKASVDYAVKPAEAGVAFSLIDPIK
                     TTPPSYELYGYGGYTLAIDPATSLYVRADYKNDLAGAQKLYPYVILTNKSIANTSLNI
                     EYNAGGTDYKGKQDVLAKNYGTLVVWAKITL"
BASE COUNT          384 a          403 c          356 g          401 t
ORIGIN      
        1 ggacaggctt cccatcggga gctacccagt ccacaggctc gaataggtat gcgaccgcga
       61 ctatgtcttc ttgaagactc atgctgtcga acaatctgac atgcggaatc gcaatcccat
      121 tcccaattgc tgtgctcatc atatcttctc gggagagaag ggcatcaaac agagcctcac
      181 gatcgaggct gttaggtaga ggcgcagatc ctataagctt gttgagtgct tttgccttat
      241 cttcaggatc aacaaggtac ataacattgg gatcattcat tcgctcgcca ataatcataa
      301 catcctcaga ttttcacaat tcaagaactg cccgcacatg gataacgaaa agcattcttt
      361 ttctcattag tctggtcatc aaaaaaaggc cgtccaaaac ggacagcctt ccaaaatctg
      421 ttttttcgtt ctacgctcta gccaaattag agagtaatct tcgcccagac aaccagcgtt
      481 ccgtaattct tagcgagcac gtcttgcttg cccttataat cggttccgcc agcgttgtat
      541 tcgatattaa gtgaggtatt tgcgatcgat ttattggtga gaatcacata aggatagagc
      601 ttctgcgcgc cagcaagatc gtttttgtaa tccgcgcgga catataggct tgtggctgga
      661 tctatggcca gcgtgtagcc gccatacccg tacagctcat agcttggagg cgttgtctta
      721 atcgggtcaa tcaaggagaa cgcgacacct gcttcggccg gtttcactgc atagtcaaca
      781 cttgcttttg cttcaacatc cttatctagt gtgctataat atccatccaa accagtctta
      841 agcccttcaa tgacagtgta gctgatcttg agcagcgcat ctgcgtcaaa cttgctcttc
      901 gcgctcacca gctgcaaatc gcttgccacg cttacagaga gaggaccctg cgcaaagctg
      961 accttggcgg ttcccgcgaa atcctttgta gcatcgaacg catcataggc cgcggcaccg
     1021 ctgagagaca gtgcatcgga aaccttatag gatgcgttgg ctccgagcgc atactcgttg
     1081 gccgcgttcg cagtccagtc atctttggat gcagcggtcg ccccaaagcc gagcgtgcca
     1141 tcattataag aaacgctcgt tccaaattta tttgcatcga tcagagcagt agtattgaca
     1201 tcgccatctt tgatagcttt tgcgttgttg tatccgaggg cactgtagga gtccactgaa
     1261 accgcccata tgccattggt gatcttggcg ctgagggatg cgggattgcc atcgtcatca
     1321 gtatcagtga atccaaggac agaagcattc aacacatcat cagtgagctt gaaggtgata
     1381 ttggtgaccg caatctcgcc ccaccagcca gtctcaccgg ctttctttgc atcctcaacg
     1441 gcaaaaggaa tcgttactgt cgcagataat ttattcttaa aaccagtata gcccgtatcc
     1501 aaatcccagc cccagctgag tgtggaactc gctgagaggg tcgg
//
LOCUS       DAOOWY010000239         1489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_85514_length_1489_cov_7.281729, whole genome shotgun sequence.
ACCESSION   DAOOWY010000239 DAOOWY010000000
VERSION     DAOOWY010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85514_length_1489_cov_7.281729"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PK105_09530"
     CDS             <1..491
                     /locus_tag="PK105_09530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU62000.1"
                     /translation="FSHIMAGAKIGKNCSLGQNVNVASNAVLGNGVKVQNNVSIYDGV
                     VIEDEVFCGPSMVFTNVINPRAFIERKHEYKKTIVRRGASIGANATIICGVTLGAYCF
                     VGAGSVVNKDVPDYALVYGNPARQHGWVCACGVKLNQELTCPECGKKYRQAEKGIERA
                     NE"
     gene            484..>1489
                     /locus_tag="PK105_09535"
     CDS             484..>1489
                     /locus_tag="PK105_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOU62001.1"
                     /translation="MSSQELLSKIEKNEAIVGIIGLGYVGLPLAVSFAKKGIRVLGIE
                     KSQKKVSLVNAGQNYIQDADNAVVNACVRKGTLSATTDALRIRECDALIICVPTPLDH
                     FKKPDMSFIESACIDIGRNMKKGVFISLESTTYPTTTEDIVKPIVERESGFKEGEDFW
                     LCFSPERVDPGNKDYKTENTPKVVGGLGEEAQKIALALYGKAIQKLYPVSSPRVAEMV
                     KILENTYRLVNISLINELALLAGKMNINIWEVIQAAKSKPYGFQAFYPGPGIGGHCIP
                     LDPFYLEYIAKGFNFDLTMIDAAGRINNLQPYRMMNKISYALNRQGKPMNGSTILFLG
                     V"
BASE COUNT          402 a          331 c          392 g          364 t
ORIGIN      
        1 atttttcgca tatcatggca ggtgcaaaga tagggaagaa ctgctccctg ggtcagaatg
       61 tcaatgtggc cagcaatgca gtgcttggca atggcgtaaa ggtacagaat aacgtctcca
      121 tctatgatgg tgttgtcatc gaggatgagg tattctgcgg cccttctatg gtctttacga
      181 atgtcatcaa tccccgcgct tttatcgagc ggaagcacga atataaaaag acgattgtac
      241 ggcgtggcgc ttctataggc gccaatgcaa cgatcatctg tggcgttacg ttaggcgcgt
      301 attgctttgt tggtgcaggg agtgtggtca ataaagatgt gccagactat gcgcttgtct
      361 atggcaatcc tgcgcgccag catggttggg tatgcgcctg tggcgtaaaa cttaatcaag
      421 aattgacttg tccagaatgc ggcaagaagt atcggcaagc tgagaaagga atcgagagag
      481 cgaatgagta gtcaagaatt attgagcaaa attgaaaaga acgaagcgat tgtggggatt
      541 atcggccttg gctatgttgg ccttccgctc gccgtgagtt ttgcaaaaaa agggattcgc
      601 gttctcggaa ttgaaaaaag ccaaaagaag gtttcgcttg tcaatgcagg gcaaaattat
      661 atccaagatg cggacaatgc agtagtcaac gcatgtgtac gcaaaggaac actttcagcc
      721 accaccgatg ccttgcgtat tcgcgagtgc gatgccctca tcatctgcgt accgacgccg
      781 ctcgatcatt tcaaaaagcc tgatatgagc tttatcgagt cagcctgtat cgatattggt
      841 cggaatatga aaaagggcgt attcatcagc ctcgaaagca ccacgtaccc taccacaacc
      901 gaagatatcg taaagcccat tgtggagcgt gagtcgggct ttaaagaggg cgaagacttc
      961 tggctctgct ttagcccgga gcgcgttgac ccaggcaata aggactataa aacagagaat
     1021 acccccaagg ttgtgggtgg tcttggagaa gaagcacaaa agatcgcgct cgcgctgtac
     1081 gggaaagcga tccaaaagct ctatcctgtg agttcaccgc gggtcgcaga aatggtgaag
     1141 atcctcgaga atacctatcg gcttgtgaac ataagcctca tcaacgagct cgcgctgcta
     1201 gcggggaaga tgaacatcaa tatctgggaa gtgatccaag cggccaagtc caagccctat
     1261 ggctttcagg ccttctatcc cggtcctggt attggcggcc actgcattcc gcttgatcca
     1321 ttctacctcg aatatatcgc aaaaggattc aattttgacc ttacaatgat cgatgcggca
     1381 ggtcgaatca ataatttgca gccgtatcgt atgatgaaca agatttccta tgcactcaat
     1441 cgtcagggca agcctatgaa tggttctacg attcttttct tgggtgtgg
//
LOCUS       DAOOWY010000240         1474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_86789_length_1474_cov_7.491896, whole genome shotgun sequence.
ACCESSION   DAOOWY010000240 DAOOWY010000000
VERSION     DAOOWY010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86789_length_1474_cov_7.491896"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..>1474)
                     /locus_tag="PK105_09540"
     CDS             complement(30..>1474)
                     /locus_tag="PK105_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOU62002.1"
                     /translation="SRAPDGEIDAGRGFWPGLCTSVKHSSRFASFCLLSKSDYKHELD
                     IRFPFCLVDNDETEGRLVLAPAWWWHSNTYALMRNEKKFKERDKRIDKSIRFIYSPFA
                     PDTMTEILAGLAILECATGKAAIESLVDNEENRRLFGYKILELVSKLRSQSSQESKAE
                     NEEKKKNKSAQRPYMQNLDIHNTNISDIHNLGALILAKQNDPLPFEVHVEGFENSKRQ
                     QIVLKPCTARTSYREMLLWFSVTTLLPVLLTELDKMDKLAGSDEILSGLLRCPERENK
                     EFEKAENSQQYRALLREPPEIEWDNMGGFLVGHSKLEDLIDRIETGGIRSWDELHREY
                     HRLSLEYEEDARAFAWAVLGYLSLDSERASAINVAIAKGDTSNLGEQWPKPSFSELIS
                     SLEAAKQLSCAIAQRVYATRAKDWTNPFRKTTFRNENEQLAVHGRIEDNSAIKDVSHE
                     MESMVVEIDRLISRLLKSDPKASHPQDGAL"
BASE COUNT          355 a          353 c          337 g          429 t
ORIGIN      
        1 caattgcgag tatggcgagt atggcactat tacaatgccc catcttgtgg atgagaggct
       61 tttggatcgc tttttaggag cctgctaatc agtctgtcta tctccaccac catggactcc
      121 atttcatggg agacatcctt tatagcacta ttatcttcta ttcggccatg tactgcgagt
      181 tgttcatttt catttcggaa ggttgtcttt ctgaaaggat tagtccaatc ttttgcacgg
      241 gtagcataga cgcgctgggc gatagcgcaa gatagttgct ttgcggcttc gaggctagag
      301 ataagctcag aaaaagaggg ctttggccac tgttctccca aattcgatgt atctccttta
      361 gcaatggcca cattgatggc actagccctt tcagaatcaa ggcttaagta gccaagaaca
      421 gcccaggcaa aagctcgagc atcctcttca tattccaagg agagtctatg atactcacga
      481 tgaagctcat cccagctacg gataccccct gtctctatgc gatcgattag atcttcaagc
      541 ttggaatggc ccacaagaaa acctcccatg ttatcccatt cgatttctgg aggttctcta
      601 agcaaagccc tatactgctg gctattctcc gccttctcga attccttgtt ttctcgttct
      661 ggacatcgca atagcccgct caaaatctca tctgatccgg caagcttatc catcttgtct
      721 aattctgtta aaaggacggg caggagagtt gtcacagaaa accagagcaa catttcacga
      781 taggaagttc ttgctgtgca tggctttaga acgatttgtt gtcttttgga attctcgaag
      841 ccctctacat ggacttcaaa aggcagcggg tcattttgtt tggcaaggat gagagcgccc
      901 aggttgtgaa tatctgagat atttgtgtta tgaatatcaa gattctgcat ataaggcctt
      961 tgtgcggact tgttcttctt cttttcttcg ttttctgcct ttgattcttg gctcgactgc
     1021 gatctgagct tgctcacaag ttcgaggatt ttgtagccga atagccttcg attttcctcg
     1081 ttgtcgacca gcgattctat ggctgctttt ccggtggcgc attcaagaat cgcgaggcct
     1141 gcaagaattt cggtcattgt gtctggcgca aaaggagaat agataaagcg gatgctctta
     1201 tcgattctct tatcgcgctc cttgaatttc ttttcatttc tcatgagcgc ataggtattg
     1261 gaatgccacc accaggcggg agccaataca agcctgccct cggtctcatc gttatccacg
     1321 aggcagaagg gaaaacgaat atcgagctcg tgcttatagt cactcttcga aagaaggcaa
     1381 aacgaagcaa agcgcgatga gtgtttcacg gaagtgcaaa gccccggcca aaaacctcgc
     1441 cctgcatcga tttctccatc gggtgcgcgc gaat
//
LOCUS       DAOOWY010000241         1430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_90727_length_1430_cov_6.163636, whole genome shotgun sequence.
ACCESSION   DAOOWY010000241 DAOOWY010000000
VERSION     DAOOWY010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90727_length_1430_cov_6.163636"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PK105_09545"
     CDS             complement(<1..813)
                     /locus_tag="PK105_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOU62003.1"
                     /translation="MPEIKELQYWIDGGWQRSTTDKYMDCYDPSTGEVIARAPKCTSE
                     EVEATIASAKKAYPAWSNTPPNKRVQVLFRMKALLDKHLDELTLMVAKENGKVLDEAM
                     GDVLKVTEVVEFACGITHLMKGPALMNCSVGYDTTQYMESIGVFAGIAPWNFPAMIPM
                     GWMAPLCIATGNTMIIKAASFCPETSMRIMELWAEAGLPPGVLNLITCSRNESEIFLR
                     HPDVKGISFVGSTSVGRHIYEVAAANGKRVQCLTEAKNHALVLKDAALERTAR"
     gene            1238..>1430
                     /locus_tag="PK105_09550"
     CDS             1238..>1430
                     /locus_tag="PK105_09550"
                     /inference="COORDINATES: protein motif:HMM:NF015884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOU62004.1"
                     /translation="MKLSQWNGDLGGIDYVSLIVIDIDGRMRAVSLPSSFADEAVLKK
                     GIGFDASNFGYAKVHASDMV"
BASE COUNT          337 a          347 c          315 g          431 t
ORIGIN      
        1 tcgagccgtc ctctctaagg ccgcatcctt caataccaga gcatggttct ttgcctcggt
       61 aaggcattgc actcgcttcc cattagccgc cgctacttcg tagatatgcc tgcccacact
      121 cgtggaacct acaaagctga tcccttttac atcgggatgg cgcaagaata tctctgattc
      181 attcctggag cacgtaatta ggttcagtac tcctggcggc agccctgctt ccgcccagag
      241 ctccattatc ctcatagagg tctcgggaca gaaactcgct gccttgatga tcatcgtgtt
      301 gccagtcgcg atgcataagg gagccatcca gcccataggt atcattgcag gaaaattcca
      361 gggcgctatg cctgcaaata ccccaatgct ctccatatac tgtgttgtgt catatcccac
      421 cgaacaattc atgagcgcag gccctttcat taaatgcgtt ataccgcaag caaactccac
      481 aacctctgtc actttaagga catcgcccat agcttcgtcg agtaccttcc cgttctcttt
      541 tgctaccatg agcgtaagct catcaagatg cttgtccagc agcgccttca tcctaaaaag
      601 cacctgtact cgtttgtttg gcggcgtgtt cgaccacgca ggataggcct tctttgctga
      661 ggcaatggtg gcctctactt cttcggatgt acatttcggc gctctcgcga tcacttctcc
      721 ggtcgaagga tcatagcaat ccatatattt atctgtggta gatctctgcc atccgccatc
      781 tatccaatat tgaagttctt ttatctcggg catgcctctt tactcctttc tgcttgtatt
      841 cttgtaacag accatactat tgtcagtctg atatagatag atatcgaatg tcaatgctca
      901 gttattattg gttttataat tggtttttgt cgcttccttg cttaaaattt aatctggctt
      961 aatctgactt aatctggctc aatctgtttc gatctggctc gaccatttgg agattcttgt
     1021 attatcttcc gaagatgttt catttattga tactatcgtt tcgtaacttg caggatgaat
     1081 gatctcggtt tacaatatgg agacgcggaa aggtcatcat aaaatttgtc ttgatttata
     1141 gactcagcga ggtcaattat tagagaagat agcctgagag taatggcacg atgagtcgct
     1201 cactaagccg cgcgcgcggc acagcgagga gttttgcatg aaattatctc aatggaatgg
     1261 cgatttgggg ggtatcgact atgtatcgct catagttatc gatatcgacg gacggatgag
     1321 agcagtgagt cttccatctt catttgctga tgaggcggtg ctcaaaaagg ggataggatt
     1381 cgatgcctct aattttggat atgcaaaagt gcatgcatcg gacatggtag
//
LOCUS       DAOOWY010000242         1361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_97649_length_1361_cov_37.206738, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000242 DAOOWY010000000
VERSION     DAOOWY010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97649_length_1361_cov_37.206738"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PK105_09555"
     CDS             complement(<1..521)
                     /locus_tag="PK105_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU62005.1"
                     /translation="MENRPRFFRKDYTTRIGASALEGALVASLEWYWASTYLYRRISS
                     ASNQAYFTRYFLQSQYFGTRIIEPWFAYPLGPDRMMFSGGIRYEKGKVAASLGGSFLI
                     LGAESEVQTYAKPYAKDWMGPQSPVTYNAQAKIALEYLASSSTLLWSCFSIEGTIENT
                     AILNFSLGLVWRLG"
     gene            complement(511..1029)
                     /locus_tag="PK105_09560"
     CDS             complement(511..1029)
                     /locus_tag="PK105_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU62006.1"
                     /translation="MLKNKLGFALTEGIMYQSADNTLDLRILNPAAIYHDYYIRGNSN
                     SILSGELNYSPISYVNLYGQMVVDEFVLPGEPLLGEPRLNHIIAIPMFSNAFEISYTE
                     DFMGYQYGCDAIVYNGNIGYKQFGKWYVSMNLFYIVHGTHDKWTLWADVSSASLYVST
                     PSTQHNIGNNGE"
     gene            complement(1011..1223)
                     /locus_tag="PK105_09565"
     CDS             complement(1011..1223)
                     /locus_tag="PK105_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU62007.1"
                     /translation="MLENIDIARLSGGLLTTYQYASSELNSSADTVKFGLGASIETYW
                     HSNTTDFLDESDWVVGFEDGTCSRTS"
BASE COUNT          380 a          288 c          323 g          370 t
ORIGIN      
        1 ccaagccgcc atacgagtcc tagggagaaa ttcaagatag ctgtgttttc tatggtgcct
       61 tcaatcgaaa aacaagacca gaggagagtt gaggaagaag caaggtattc taaggcgatc
      121 ttagcttgtg cgttatatgt gacggggctt tggggtccca tccaatcttt ggcatagggt
      181 ttggcatatg tctgcacttc ggattctgcg ccgaggatca agaagcttcc gcccagcgat
      241 gccgcgactt tacctttttc atacctgata ccaccggaga acatcattct gtctggaccg
      301 agaggatatg caaaccaggg ttcaataatg cgcgtgccaa aatactgaga ttgaagaaaa
      361 taccgcgtaa aatatgcttg atttgaggca cttgaaatgc gtcgatacag gtaagtcgaa
      421 gcccaatacc attctagaga tgctacaagg gctccctcga gggctgatgc tccaattcta
      481 gttgtataat cttttctgaa aaatcgaggt ctattctcca ttgttgccta tgttgtgctg
      541 ggtgcttggt gtcgagacat atagactcgc gctggaaaca tcagcccaaa gcgtccactt
      601 gtcgtgggtt ccatgtacta tataaaagag attcattgag acataccatt tgccgaactg
      661 cttgtatccg atattgccat tgtagacaat tgcatcgcat ccgtattggt agcccataaa
      721 atcttctgta taggaaattt cgaaggcatt tgaaaacata gggatagcaa taatatgatt
      781 gagcctcggt tcacctagta ggggttctcc aggaagcaca aattcatcta ccaccatctg
      841 cccatagaga ttgacatagg atataggcga ataattgagc tctccagaga ggatggaatt
      901 cgaattgccg cgaatgtagt aatcgtggta gatcgcggct ggattcagta tacgcaaatc
      961 aagggtattg tcagccgatt gatacattat tccttcggtg agggcaaatc ctagcttgtt
     1021 cttgagcatg ttccatcctc aaaaccgact acccagtcgg attcgtcaag aaaatcagta
     1081 gtattgctat gccagtaggt ttcgatgcta gcgcctaacc caaacttgac agtatctgca
     1141 ctactgttta gctctgaaga cgcatattga tatgtggtca acaaaccacc cgataacctg
     1201 gctatatcta tattttcgag catgttcaaa agctcatcgg cactccatgg cccagtggtg
     1261 gaaggaaggg caagcacttg gcttatatag agtggcttat atagagtggc ttatatagag
     1321 tgcggttata gcttcataga catcggaatc tatgggatag a
//
LOCUS       DAOOWY010000243         1343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_99593_length_1343_cov_15.980590, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000243 DAOOWY010000000
VERSION     DAOOWY010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99593_length_1343_cov_15.980590"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..872)
                     /gene="msrA"
                     /locus_tag="PK105_09570"
                     /pseudo
     CDS             complement(381..872)
                     /gene="msrA"
                     /locus_tag="PK105_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882079.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
     gene            1155..>1343
                     /locus_tag="PK105_09575"
     CDS             1155..>1343
                     /locus_tag="PK105_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU62008.1"
                     /translation="MKSIADVLGSLHIIAIPTLHPESSRHSHIMKRCDRSNRNNWKAR
                     FSISNALRANPSHPDGPRR"
BASE COUNT          378 a          279 c          303 g          383 t
ORIGIN      
        1 ttaaagagcg ctacgatccg caaaaggtgc attgggaaaa gtggtgaagg aaaacatatt
       61 tgccctggag actattgata attgattatc ctattatcat cttattatgg cataggtgat
      121 tactagtata gatctgttta tgacgatctc atctttccaa attcccaaaa agatacatag
      181 cgcttattgg gacgcgcata ttcaaaataa tacggccatt tacctatact tccaaataca
      241 atttaacatt gaaagaatcc ttaaactata tcgaaggaat cagcttagtg ccaaaactta
      301 aaaatgactc tttgggagtt gcgattgcat atactattgc tcttaattaa aagagaatat
      361 cagagcatct tttagcggct tcactccaaa ggcttagctg caaggctcaa gtctacatgg
      421 cagtagccgc caggattctt acgtaggtaa ttctggtgat attcttcggc tgggtagaag
      481 ggcatggttg gcacaagttc tgtaacgatg ggctttgagt atttcttgct cgcggagagc
      541 tcggcaatga ttttttcagc aatgacgcgc tgctcgtcgt cctgatagaa gattacagat
      601 cgatattgag tgccgaaatc agcgccctgt cggttgagct gggtcggatc gtgcatgcga
      661 aaaaagtggc gcaggagttg gtcatagctt atcttggcgg ggtcgaactc gatacggagc
      721 gattcggcat ggttgctggt accactgcag acttgctcgt aagtaggatt tggggttgtg
      781 cctccgcagt accccacggt agtcgagagt acgccaggca aacggcggaa atactcttca
      841 gtaccccaga agcaacctgc agcaaagatg gcgacctgcc tcttgtctac atcggtttgc
      901 atgggcgcgc tttgagatga attcctggat atattcacgt tttctcctct cttttattct
      961 aaagatggat agtgtgtttc aagactttag atggatttac aaaatatcct cagctcgatt
     1021 tagaaaatac caatagtggc tattccaaag gtcattcaga gatgtaattc tctaatgcaa
     1081 agcggggagg aaccttgcat aattgagtaa ttagaagacc caatacccaa tatagaagga
     1141 aaaaagtctc tataatgaaa agtatagcag atgttctggg ttcattacat ataattgcta
     1201 ttcccacttt acatccagag tcatcccggc attctcatat catgaagcgc tgcgatcgct
     1261 ccaatcgcaa taattggaag gctcggttct ctatttcaaa tgctcttcga gccaatcctt
     1321 cacatcctga tggacctcgt cgc
//
LOCUS       DAOOWY010000244         1338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_100163_length_1338_cov_21.349961, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000244 DAOOWY010000000
VERSION     DAOOWY010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100163_length_1338_cov_21.349961"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..946
                     /locus_tag="PK105_09580"
     CDS             53..946
                     /locus_tag="PK105_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU62009.1"
                     /translation="MDTIKSKYAHLVGEIVAHVPKEARVSELDVYQEGISQILPFIDA
                     IIADHLEDGSRIEGLDNLKNLYEHAKNGESTLLLMEHYSNFDLPVFHYLLRKEGDEGK
                     QIASNLVAIAGIKLNEENPAVNAFTRAYTRIVIYPSRSMQIIRERLTDPIELYQEMKR
                     SISINRAAMRALDRARHNGKMILVFPAGTRYRPWDPSSKRGVREIASYVKSFSKFGLI
                     SINGNILRLNPSGAMEDDILQKDRVIYNISPVHDNREFLNSVRYDLRFFEDKKQAIVD
                     TIMDLLEDMHYEVENRRFIDR"
     gene            complement(933..>1338)
                     /locus_tag="PK105_09585"
     CDS             complement(933..>1338)
                     /locus_tag="PK105_09585"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU62010.1"
                     /translation="GLYFFGRPVEHSLGSYEFLLYYLGSGLFAGIASFAIYLLSGAWN
                     TYLLGASGAVFSLLLAFAVLYPRAMVYVYGIIPLRAPVMVIGYTLIEILSSFLGAASS
                     VAHLTHLAGFAAGAVYFPIRLGVNPFKRLSDR"
BASE COUNT          422 a          328 c          285 g          303 t
ORIGIN      
        1 gataaattga cacctcttga taaaacacct atagtctaga atcaaggatg ccatggacac
       61 aatcaagagc aaatacgctc atctagtggg cgaaatagtt gcccatgtcc caaaggaagc
      121 tcgtgtctct gagctcgatg tctatcagga aggcataagt cagattctcc ctttcatcga
      181 tgcgattatt gcggaccatc tggaagatgg ctcgaggatc gagggtttgg ataacctcaa
      241 aaacctatac gaacatgcca agaatggaga atccacacta ctcttaatgg agcattattc
      301 gaacttcgac ctgcctgtat ttcattatct cttgcgaaaa gagggagacg aaggcaaaca
      361 aatcgcttca aacctagtgg caattgctgg aatcaaactt aacgaggaaa atccagccgt
      421 caacgccttt acgcgagcat atactcgaat agtgatctat ccttcgcgat ctatgcagat
      481 tataagggaa agactcaccg acccgatcga gctataccaa gaaatgaaac gaagtatatc
      541 gataaatcgc gccgcaatga gagccctcga tcgcgctaga cacaatggca aaatgatcct
      601 tgttttccct gctggaacgc gctatcggcc atgggaccct tcctctaagc gcggcgtccg
      661 cgaaatagcc tcttatgtca agtctttttc caaatttggc cttatatcaa tcaatggcaa
      721 cattctcaga ctcaatcctt ctggcgctat ggaagacgat attctgcaga aggatcgggt
      781 aatctacaac ataagccctg tccacgataa cagagaattc ttaaattctg ttcgatacga
      841 tcttcgattt ttcgaagata aaaaacaggc cattgtagac accataatgg acctcctcga
      901 agacatgcat tatgaggtgg agaaccgcag gtttatcgat cgctgagcct tttgaaggga
      961 tttaccccta agcgaatagg gaaatagacc gctcctgccg caaaaccagc cagatgagtg
     1021 agatgtgcta cggagctcgc cgctcccagg aaagacgata atatctcgat caatgtatat
     1081 cctataacca tgacaggagc tctaagagga attatgccat acacatatac catcgccctc
     1141 gggtatagca ccgcaaatgc aagcaatagg gaaaaaaccg cccccgatgc gccaagcaga
     1201 tatgtattcc atgcgcctga aagaagataa atcgcaaaag aggcaatgcc tgcaaacagt
     1261 cccgatccaa ggtaatagag cagaaattca taactaccaa ggctatgctc caccggccta
     1321 ccgaagaaat atagcccc
//
LOCUS       DAOOWY010000245         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_118537_length_1195_cov_12.071053, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000245 DAOOWY010000000
VERSION     DAOOWY010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118537_length_1195_cov_12.071053"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PK105_09590"
     CDS             <1..1078
                     /locus_tag="PK105_09590"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU62011.1"
                     /translation="LLHEIHYAPRDLSHPYWRYEHPQEWNEIREAMPAATVSETGRPS
                     LPSDLEARIHAGWLGQLAGGAFGTAIEGYHSEQLHKVYGRIDEYITEPETTNDDVVYE
                     LAFLDALEKNGRNMTSCDIALEWIRQIPFGWSAEWIALHNLADGIFPPESGSWHNPYS
                     NWIGAQMRGMVCGLVSPGWPMEAARLAYLDGVVSHAENGVYGEIYAAVLTSLAFNLSN
                     PRTLIINAAEFIPAKSEYAAVLTKCFETVRSCAEPEAAWQELDIYFERYNWIHAYPNI
                     AAVITALWFGNCDMTESFRILAHAGLDVDCNAGLVGTVIGIINGISDKWGKPLNDTLE
                     TYLAGKEHLSIQALANTTARLATL"
BASE COUNT          311 a          309 c          279 g          296 t
ORIGIN      
        1 gcttctccat gaaatccact atgcgcctag agacctctcc catccatatt ggcgctatga
       61 acatccacaa gaatggaatg agattcgcga agcaatgcct gccgctaccg tctccgagac
      121 cggtcgccct tctctaccca gcgatttgga agccagaata catgctggct ggcttggcca
      181 acttgcaggt ggcgcattcg gtaccgcaat cgaaggttat catagcgagc aattgcataa
      241 ggtctatgga cgcatagacg agtacataac agaaccagaa accacaaacg acgatgtggt
      301 ctatgagctc gcctttctcg atgcgctgga aaaaaatgga agaaatatga catcgtgcga
      361 cattgcgctc gagtggatac gccagattcc ctttggatgg tctgccgaat ggatagcatt
      421 gcacaacttg gccgatggca tattccctcc agaatccggc tcttggcaca acccctactc
      481 gaattggata ggagcgcaga tgcgtggcat ggtatgtggc cttgtgagcc ctggctggcc
      541 catggaagct gctcgccttg cctatctcga tggcgtagta tcgcacgcag aaaatggagt
      601 ctatggtgag atctatgcgg cagttttgac ttctctcgca ttcaacctat cgaatccaag
      661 gactcttatt atcaatgccg ccgaatttat acctgcaaag agtgaatatg ctgccgtgct
      721 tacaaaatgt ttcgagactg tccgctcatg cgcagagcct gaagcagcct ggcaggaact
      781 cgatatatat ttcgaacgct ataactggat tcatgcttac cccaatattg cagctgtcat
      841 caccgcgcta tggtttggga attgtgacat gaccgaatcc ttccgcatcc ttgctcatgc
      901 aggcctcgat gtggactgca atgcaggtct tgtggggact gtcatcggaa taataaacgg
      961 catatcggat aaatggggaa aacccttgaa tgatacactc gaaacttatt tagccggcaa
     1021 agagcacctc agcatacaag cgcttgcaaa tacaacggct cgccttgcga ctctataaag
     1081 cgctatagag ccctatactt atctttcatt cggtactcgg ccgtacgcag cgcctaaaag
     1141 acccatattt attcgagatt attgacacta tatgcaattc gccatattct cctca
//
LOCUS       DAOOWY010000246         1109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_132880_length_1109_cov_11.717268, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000246 DAOOWY010000000
VERSION     DAOOWY010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132880_length_1109_cov_11.717268"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1060
                     /locus_tag="PK105_09595"
     CDS             59..1060
                     /locus_tag="PK105_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU62012.1"
                     /translation="MTSIKDIARLAGVSPSTVSRVLNEREYVRDEVRQRVLSIVKEKG
                     YVQNNVARSMVLKRSFTIGVVIPHLFNMFQRQLFASMEFFLEQCGYKTQFFFIRWSEA
                     SEQAFLRRLKSETLDGIIFIHELDSPAVYQHIQERGLPVALCTFEKEQFDFPAVHIEE
                     EASAALAIEYLIQKGHSNIGLITGGHFSFGKQREEGYRQALKAHGIPCNPELIVRVPS
                     YNPEAGRSGMITLLNRRSPMTAVFAATDELAIGAIKSLYDSGLRVPEDISVIGLDDID
                     ISAFTTPGLTTVHQPIFEMGKKSADLVCDWIARGSMQGKVELFSTTIVERESVRALA"
BASE COUNT          294 a          258 c          277 g          280 t
ORIGIN      
        1 gcacagacaa aagctctgtc tctagatgaa aatcttttca gaggctatga taaaatatat
       61 gaccagtatc aaagatatcg ccaggcttgc tggtgtttcg ccttctaccg tttcgcgcgt
      121 ccttaatgag agggaatatg tgcgcgacga agtgcgtcaa cgcgtgctca gcatcgtcaa
      181 agaaaaagga tatgtgcaga acaatgtagc gcggtcgatg gtactcaaga gaagctttac
      241 aattggcgtt gttatccctc atctgttcaa tatgtttcag agacagctct ttgcatcgat
      301 ggagtttttc cttgagcaat gtggttataa gactcagttc tttttcattc gatggtctga
      361 ggccagcgaa caagcttttc tgcgccgact aaaaagcgag acgctagatg gcataatatt
      421 tattcatgag ctagacagcc cggcggtcta tcagcatatc caagagagag gcttgcctgt
      481 cgcgctatgc acattcgaaa aggagcaatt cgatttccct gcggttcaca tagaagaaga
      541 agcctccgct gccctggcga tcgaatacct tatacagaaa ggacacagca atatcgggct
      601 cattacagga ggacacttta gtttcggaaa gcagcgagaa gaaggctatc gccaagcgct
      661 caaggcgcat ggtattcctt gcaacccaga actcattgtt cgcgttcctt cctacaaccc
      721 cgaggctggc cgctcgggta tgataacact cttgaacaga agatcgccta tgactgcggt
      781 gtttgctgca acagatgagc ttgcaatagg cgcgataaaa tcgctttatg attcaggcct
      841 tagagtgccc gaagacatca gtgtaattgg tctcgatgat atcgatattt cagcctttac
      901 gactcctggc ctgaccacgg tccatcagcc gatcttcgaa atgggcaaga agagcgccga
      961 tcttgtatgc gattggattg ccagagggag catgcaagga aaagtagagc ttttctcaac
     1021 aaccatcgta gagcgcgaat cagtacgggc attggcttag agtgatttta atcctctgga
     1081 gatcggcgcc cttgcggccc ttccgatgg
//
LOCUS       DAOOWY010000247         1101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_134396_length_1101_cov_5.302103, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000247 DAOOWY010000000
VERSION     DAOOWY010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134396_length_1101_cov_5.302103"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PK105_09600"
     CDS             complement(<1..1054)
                     /locus_tag="PK105_09600"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOU62013.1"
                     /translation="MPIIYIEGAISGDIVGISTGDQTVAQTNYAVLRTSYREGITVST
                     LLRPLKDKILASANMQQAFIIRKGQETQIMINLEKLLYGTDLNGDVALKPEDKIVIPY
                     GSMYVFVTGEVTKSSWVGITGLTRLRDAVTPLLNRYSSIRDVKVKSADGYEKTFDLFK
                     AERYGDLSQDPFLRPGDEIIISSLTNLVTIQGEVKRPGTYQLLPGEGLKELVSVYADG
                     FTEKANTSRLTLLHYLSESSPVGEKIQFDYTKFPDMELRLYDIVAVPSLQELLPVAWF
                     EGAVGLSSTGASPETSQRLAYTFVPGETVSQAAHANRKLFSAVSDLASAYIIHVDGSQ
                     TAVNLAKFIYDYDLTND"
BASE COUNT          282 a          256 c          245 g          318 t
ORIGIN      
        1 tatcatttgt caaatcataa tcgtagatga attttgcaag attgaccgct gtctgcgatc
       61 catctacatg tatgatatat gcggaggcca gatcggatac agctgaaaag agttttcggt
      121 ttgcatgagc tgcttgggac actgtttctc caggcacaaa ggtgtatgcc agtctctggc
      181 tggtttcagg tgatgctcca gtgctactaa gtcctacggc tccctcaaac catgctacag
      241 gcaagagctc ctgcaatgaa ggaacagcca ctatatcgta gagccttagt tccatgtccg
      301 gaaatttggt ataatcgaat tggatttttt cgcccacggg cgagctttcg gagagataat
      361 gcaacagcgt gaggcgcgaa gtattggctt tttcggtaaa gccatctgca tatacgctca
      421 caagctcttt gagaccctcg ccaggtagaa gctgataagt tcctgggcgt tttacttctc
      481 cttgaattgt cacaagattc gtaaggctag aaattatgat ctcatcgccg ggcctcaaga
      541 agggatcctg cgaaagatcc ccatagcgct ctgccttgaa aagatcaaag gttttttcgt
      601 agccatcggc agatttgacc ttgacatcgc gaatagaaga atatcggtta agaagcggtg
      661 taacggcatc tcgcaagcgt gttagacctg ttatgcctac ccagctcgac ttggtcactt
      721 ctccagttac gaagacatac atcgagccat aggggattac gattttgtct tctggcttga
      781 gcgcgacatc tccatttaag tcagtaccat aaagtagttt ctcaagattt atcattattt
      841 gtgtttcttg gcctttgcgg attataaagg cctgctgcat attagccgat gcaagaatct
      901 tatctttcaa aggtcgcaaa agagtcgata cggttattcc ttctcgatac gatgttctaa
      961 gaacagcata gttcgtttgg gcaactgtct ggtcccccgt cgatattcct acaatgtcgc
     1021 cggaaatagc tccttctata taaataatgg gcaaatactc ttccctactc gctaccctta
     1081 tactgtcccc atcctgtaaa c
//
LOCUS       DAOOWY010000248         1043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2403
            NODE_146284_length_1043_cov_21.863360, whole genome shotgun
            sequence.
ACCESSION   DAOOWY010000248 DAOOWY010000000
VERSION     DAOOWY010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864478
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:02:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,922
            CDSs (total)                      :: 1,911
            Genes (coding)                    :: 1,902
            CDSs (with protein)               :: 1,902
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146284_length_1043_cov_21.863360"
                     /isolate="AMR_MDS_2403"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PK105_09605"
     CDS             complement(<1..547)
                     /locus_tag="PK105_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU62014.1"
                     /translation="MHTIIRTESITKEFSSVRVLNDISIEICAGEVFGIIGENGAGKS
                     TFIKVLSGLYQPTSGKIWFDDKLINMRSPADAKRIGISLIPQEFSLVSDLCVYENIFL
                     GEELVGKHGILDIKAMVNRTRQLLTDLEVSTIDPYAHIQALSVAQKQMVEIAKAIAFD
                     SRLLIMDEPTTMLTSKEIEILF"
     gene            complement(547..>1043)
                     /locus_tag="PK105_09610"
     CDS             complement(547..>1043)
                     /locus_tag="PK105_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU62015.1"
                     /translation="FDRCGVKFALEVHPTEIAFDYYTAGRLLKEFGNRPTLGFNFDPS
                     HLIWQGISPHLFIRDYASRIYHVHMKDAAVTLDGKAGILGSHMAFGDTRRGWNFRSLG
                     HGDVDFENIIRELNAAGYQGPLSVEWEDSGMDREYGAKEALDFVREVDFVPSMIAFDA
                     AMKK"
BASE COUNT          277 a          247 c          232 g          287 t
ORIGIN      
        1 caaatagtat ctctatctcc ttgctggtga gcatggttgt aggttcatcc attataagaa
       61 ggcgagaatc aaaagcgata gctttggcta tctcgaccat ctgtttctgc gctactgaaa
      121 gagcctgaat gtgtgcatat ggatcgattg tggagacttc caaatcggtg aggagctggc
      181 gcgtcctatt gaccattgct ttgatatcca aaattccatg ttttcctaca agttcttcac
      241 cgaggaatat gttttcatat acgcaaagat ctgaaacaag gctgaattcc tggggaatca
      301 ggcttatacc gattcttttt gcatcggcag ggctcctcat atttattagc ttgtcatcga
      361 accaaatctt gccagatgtt ggttggtata gtcccgaaag aaccttgatg aaagtagatt
      421 tccctgcccc attttcgcca attatgccaa agacctcccc tgcgcagatt tcgatagaga
      481 tatcgttaag gacccgaact gaggagaatt ccttggtaat gctttcggtt cttataattg
      541 tatgcattat ttcttcatcg ctgcatcgaa ggcaatcatc gagggtacaa aatctacctc
      601 tcgcacaaaa tcaagagctt cttttgcacc gtattcgcga tccatgccag aatcttccca
      661 ttccaccgag agcggccctt gatagccagc agcattgagt tcacgaataa tgttttcgaa
      721 atccacatct ccatggccta gagagcggaa attccatcca cgacgcgtat ctccgaaagc
      781 catatgagag ccaaggatgc ccgccttacc atcgagtgtc actgcagcgt ctttcatgtg
      841 cacatggtag attctgcttg catagtcgcg gataaaaaga tgcggcgaga taccctgcca
      901 gatgagatgt gagggatcga agttaaatcc aagagttggc cggttgccga attccttgag
      961 caagcgacct gcagtgtagt aatcgaatgc tatctcagtt ggatgcacct cgagtgcaaa
     1021 cttgacacca caacgatcga act
//
